Lcy12g015980 (gene) Sponge gourd (P93075) v1

BLAST of Lcy12g015980 vs. ExPASy Swiss-Prot
Match: Q9LYE3 (Protein ANTI-SILENCING 1 OS=Arabidopsis thaliana OX=3702 GN=ASI1 PE=4 SV=2)

HSP 1 Score: 428.3 bits (1100), Expect = 1.4e-118
Identity = 273/659 (41.43%), Postives = 381/659 (57.81%), Query Frame = 0

Query: 1   MVDTEEVENVEFQWGKRKSIGGKKKDVTFYESFTYDGVEYFLYDSVYLYKEGEP---EPY 60
           M ++   E +EF+WGK+K +GGKKKDV FYESFTYDG EY LYD V +    EP   EP+
Sbjct: 1   MEESVASEGLEFKWGKKKGVGGKKKDVQFYESFTYDGDEYRLYDCVLVGNASEPDSTEPF 60

Query: 61  IGKILKIWQNHDK--TKKVKILWFFRPCEILNYL-GAEDTRDNELFLASGDGVGLANVNP 120
           IG I+KIW++ +K   KKVK+LWFF+P EI  YL G  +   NE+FLASG+G+GLAN N 
Sbjct: 61  IGMIIKIWEHANKHIPKKVKLLWFFKPSEIAPYLEGVPNVLANEVFLASGEGLGLANTNQ 120

Query: 121 LEVIAGKCSVLCISNDSRNSRPSDEALKKADFVFCRTFDVGKQEVCNEICEKIAGVEVKF 180
           LE I GKCSVLCIS D RN +PSDE    ADFVFCR FDVG  +V + I +KIAGV+VKF
Sbjct: 121 LEAIGGKCSVLCISKDKRNPQPSDEKFNSADFVFCRAFDVGSCKVVDTIDDKIAGVDVKF 180

Query: 181 LLNKVDTSKDVKRTDKDGKDASGIATVNTESEDPSGQNISNGELTLKPDESSVKKST--- 240
           + N+  + K+         D +G     ++S  P+G        +++  E S  +S+   
Sbjct: 181 IFNRACSEKEATAVQNIEADVNG----KSDSLKPNGPLARGASGSVRKIEDSAFESSDCK 240

Query: 241 -------EENVDLKVSLPIEKSS-NEEKSGACASDGG---NGLANTSSKQENILDDKVSP 300
                  EE       L I+KS+  EE+S   +   G   NG    S  ++ +   K  P
Sbjct: 241 ENSNGCKEEKEKGHYQLAIKKSTLAEERSNKDSGSRGNHYNGKDQESEVKKQLTKQKSMP 300

Query: 301 KLKIDSNETPGKDVKAL----AKDDEGRVKN--------------PRDSAEVEHRPAKKA 360
             +  SN       + +     K  E  VK                ++S+ ++ RP KK 
Sbjct: 301 GEERYSNSFEASGSRTIHSISKKAQENDVKKQLTKQKSMPAGERYSQESSGLDDRPLKKQ 360

Query: 361 KLDSSVQLSHGKTKSDIQKLGLN--RNNGDTLASSPKVLVSE--------DASRTKHVKD 420
           KLD SV +  G   + +Q +  +  ++ G       KV + E          +R   V  
Sbjct: 361 KLDGSVTVRDGWDTTILQNITSDGKKDTGSFKRPRDKVTIEEVPPEKRSFVKNRDLVVSV 420

Query: 421 SQGTKDSLIKKPKLDEKPTKVSNGKNLKVSSQISSVTDCKIDGQIVEVTRRPDADRSRWF 480
           S+G     + +  + +KP   S G+    +    S  D + + Q+ EV RRPDA +S+WF
Sbjct: 421 SEGKTTKTVTEKGISKKP---SFGR----AEDKMSADDNERNYQVTEVCRRPDAGKSKWF 480

Query: 481 KGLPWEERMKNAHEQGTLVLIQNLDPGYTSGEVEDIVWHAFNESCTAKMIQRTAISMPHI 540
           + LPWEE M+ A ++GT+VL+QNLDP YTS EVEDIV+ A N+ C A+MI+RT++++PHI
Sbjct: 481 RSLPWEESMREAEKKGTVVLLQNLDPTYTSDEVEDIVYSALNQQCEARMIERTSVTIPHI 540

Query: 541 GQAYVVFKTKEAAEKVVRKLDEGCLLLANGSALVGCFATPHLPSKKQTFFGHHSIDRLRH 600
           G+A V+FKT+E AE+V+R+LDEGCLLL++G  LV  FA    P K   F GH  + +   
Sbjct: 541 GEALVIFKTREVAERVIRRLDEGCLLLSSGRPLVASFAKITPPGKPSLFSGHIKLHKT-- 600

Query: 601 QMQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSELACKKLFKQQEEELRKLKTKLK 612
           Q +REM++AV+TSH SQPN +E+DMAMEWCL Q R E A + + K+Q EE++ L+   K
Sbjct: 601 QTRREMRDAVATSHSSQPNNLEFDMAMEWCLHQARHEQASESVSKRQLEEMKSLRINFK 646

BLAST of Lcy12g015980 vs. ExPASy TrEMBL
Match: A0A6J1C0I4 (protein ANTI-SILENCING 1 OS=Momordica charantia OX=3673 GN=LOC111007160 PE=4 SV=1)

HSP 1 Score: 1015.0 bits (2623), Expect = 1.3e-292
Identity = 515/615 (83.74%), Postives = 559/615 (90.89%), Query Frame = 0

Query: 1   MVDTEEVENVEFQWGKRKSIGGKKKDVTFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK 60
           MV+ E+VEN+EF+WGKRK+IGGKKKDVTFYES TYDGVEYFLYDSVYLYKEGEPEPYIGK
Sbjct: 1   MVEPEQVENIEFRWGKRKAIGGKKKDVTFYESCTYDGVEYFLYDSVYLYKEGEPEPYIGK 60

Query: 61  ILKIWQNHDKTKKVKILWFFRPCEILNYLGAEDTRDNELFLASGDGVGLANVNPLEVIAG 120
           ILK+WQN DKTKKVKILWFFRPCEILNYLGAEDT DNELFLASG+GVGLANVNPLEVIAG
Sbjct: 61  ILKMWQNPDKTKKVKILWFFRPCEILNYLGAEDTPDNELFLASGEGVGLANVNPLEVIAG 120

Query: 121 KCSVLCISNDSRNSRPSDEALKKADFVFCRTFDVGKQEVCNEICEKIAGVEVKFLLNKVD 180
           KC+VLCISNDSRN RPSDE LKKADFVF RTFDVGKQEVCN++C KIAG+EVKFLLN+VD
Sbjct: 121 KCNVLCISNDSRNPRPSDEVLKKADFVFSRTFDVGKQEVCNDMCGKIAGIEVKFLLNRVD 180

Query: 181 TSKDVKRTDKDGKDASGIATVNTESEDPSGQNISNGELTLKPDESSVKKSTEENVDLKVS 240
           +SKDV RTDKDGKDASG   VNTE +DPS QN  NGELTL+  ESSV+KSTEENVDLKVS
Sbjct: 181 SSKDVNRTDKDGKDASGGTIVNTEFDDPSQQNTPNGELTLEKHESSVEKSTEENVDLKVS 240

Query: 241 LPIEKSSNEEKSGACASDGGNGLANTSSKQENILDDKVSPKLKIDSNETPGKDVKALAKD 300
           LP +KSSNEEKS ACASDGG+G+ANTSSK+ENIL DKVSP+LKID NE  GKDVKALAKD
Sbjct: 241 LPTQKSSNEEKSTACASDGGHGMANTSSKEENILGDKVSPRLKIDPNEIAGKDVKALAKD 300

Query: 301 --DEGRVKNPRDSAEVEHRPAKKAKLDSSVQLSHGKTKSDIQKLGLNRNNGDTLASSPKV 360
              E RV++P+ SAEVEHRPAK+AKLD+S+Q S GK K+++QK GLNRNN DT AS+PK 
Sbjct: 301 VEFEDRVRSPKGSAEVEHRPAKRAKLDTSIQSSPGKAKNNVQKFGLNRNNSDTQASAPKS 360

Query: 361 LVSEDASRTKHVKDSQGTKDSLIKKPKLDEKPTKVSNGKNLKVSSQISSVTDCKIDGQIV 420
           +VSEDAS+TK+VKD  GTKD LIKKPKLDEKP KV NG N+K  SQISS    KIDGQ V
Sbjct: 361 IVSEDASKTKNVKDYHGTKDGLIKKPKLDEKPPKVFNGTNIKSPSQISSDKSAKIDGQTV 420

Query: 421 EVTRRPDADRSRWFKGLPWEERMKNAHEQGTLVLIQNLDPGYTSGEVEDIVWHAFNESCT 480
           EVTRRPDADRSRWFKGLPWEERMK AHEQGTLVLIQNLDP YTSGEVEDIVWHAF+ESCT
Sbjct: 421 EVTRRPDADRSRWFKGLPWEERMKTAHEQGTLVLIQNLDPAYTSGEVEDIVWHAFSESCT 480

Query: 481 AKMIQRTAISMPHIGQAYVVFKTKEAAEKVVRKLDEGCLLLANGSALVGCFATPHLPSKK 540
           AKM+QRTA SMPHIGQAYVVFKTKEAAEKVVRKLDEGCL+L+NG ALVG FATPH P+KK
Sbjct: 481 AKMVQRTAYSMPHIGQAYVVFKTKEAAEKVVRKLDEGCLVLSNGRALVGSFATPHFPAKK 540

Query: 541 QTFFGHHSIDRLRHQMQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSELACKKLFKQ 600
           QTFFGHHSID+LRHQMQREMK+AVSTSHCSQPNTVEYDMAMEWCLL+ERSELACKKLFKQ
Sbjct: 541 QTFFGHHSIDKLRHQMQREMKDAVSTSHCSQPNTVEYDMAMEWCLLEERSELACKKLFKQ 600

Query: 601 QEEELRKLKTKLKSR 614
           QEEELRKLK+KL SR
Sbjct: 601 QEEELRKLKSKLISR 615

BLAST of Lcy12g015980 vs. ExPASy TrEMBL
Match: A0A6J1J1L4 (protein ANTI-SILENCING 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481035 PE=4 SV=1)

HSP 1 Score: 1012.3 bits (2616), Expect = 8.6e-292
Identity = 528/613 (86.13%), Postives = 552/613 (90.05%), Query Frame = 0

Query: 1   MVDTEEVENVEFQWGKRKSIGGKKKDVTFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK 60
           MV+TE+ E VEFQWGKRK IGGKKKDV+FYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK
Sbjct: 1   MVETEQEEKVEFQWGKRKGIGGKKKDVSFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK 60

Query: 61  ILKIWQNHDKTKKVKILWFFRPCEILNYLGAEDTRDNELFLASGDGVGLANVNPLEVIAG 120
           ILKIWQN DKTKKVKILWFFRPCEILNYLGAEDTRDNELFLASGDGVGLANVN LEVIAG
Sbjct: 61  ILKIWQNPDKTKKVKILWFFRPCEILNYLGAEDTRDNELFLASGDGVGLANVNSLEVIAG 120

Query: 121 KCSVLCISNDSRNSRPSDEALKKADFVFCRTFDVGKQEVCNEICEKIAGVEVKFLLNKVD 180
           KCSVLC SNDSRN RPSDEALKKADFVFCRTFDVGK EVCNEICEKIAGVEVKFLLNKVD
Sbjct: 121 KCSVLCYSNDSRNPRPSDEALKKADFVFCRTFDVGKLEVCNEICEKIAGVEVKFLLNKVD 180

Query: 181 TSKDVKRTDKDGKDASGIATVNTESEDPSGQNISNGELTLKPDESSVKKSTEENVDLKVS 240
           TSK VKR +KDGKD    A VNTESED   +N SNGE TLK  +S+V+ ST+ENVDLK S
Sbjct: 181 TSKYVKRAEKDGKD----AIVNTESEDLFERNTSNGESTLKTKDSTVEMSTKENVDLKAS 240

Query: 241 LPIEKSSNEEKSGACASDGGNGLANTSSKQENILDDKVSPKLKIDSNETPGKDVKALAKD 300
           L IEKSSNEEKSG C SDGGNG+ANTSSK +NILDDKVS KLKID N+TPGKD  AL KD
Sbjct: 241 LSIEKSSNEEKSGVCVSDGGNGMANTSSKHKNILDDKVSSKLKIDYNKTPGKD--ALPKD 300

Query: 301 DEGRVKNPRDSAEVEHRPAKKAKLDSSVQLSHGKTKSDIQKLGLNRNNGDTLASSPKVLV 360
            EGRVK+PRDS+EVEHRP KKAKLDS VQLS GKTKSDIQK GLNR+NGDTLASSPK+ V
Sbjct: 301 VEGRVKSPRDSSEVEHRPEKKAKLDSFVQLSLGKTKSDIQKPGLNRSNGDTLASSPKIHV 360

Query: 361 SEDASRTKHVKDSQGTKDSLIKKPKLDEKPTKVSNGKNLKVSSQISSVTDCKIDGQIVEV 420
           SE+ASRTK+VKDS GTKDSLIKKPKLDEKPTKVS GKNLK      SV DCKIDGQIVEV
Sbjct: 361 SENASRTKNVKDSHGTKDSLIKKPKLDEKPTKVSIGKNLK-----PSVADCKIDGQIVEV 420

Query: 421 TRRPDADRSRWFKGLPWEERMKNAHEQGTLVLIQNLDPGYTSGEVEDIVWHAFNESCTAK 480
           TRRPDADRSRWFKGLPWEERMKNAHEQ TLVLIQN DP YTSGEVEDIVWHAFNESCTAK
Sbjct: 421 TRRPDADRSRWFKGLPWEERMKNAHEQRTLVLIQNFDPAYTSGEVEDIVWHAFNESCTAK 480

Query: 481 MIQRTAISMPHIGQAYVVFKTKEAAEKVVRKLDEGCLLLANGSALVGCFATPHLPSKKQT 540
           MIQRTA SMPHIGQAY+VFKTKEAAEKVVRKLDEGCLLLANG+AL G FATPHL  KKQT
Sbjct: 481 MIQRTANSMPHIGQAYIVFKTKEAAEKVVRKLDEGCLLLANGNALFGSFATPHLQLKKQT 540

Query: 541 FFGHHSIDRLRHQMQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSELACKKLFKQQE 600
           +FGHHSID+LRH MQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERS+L  KKLFKQQE
Sbjct: 541 YFGHHSIDKLRHLMQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSQLTWKKLFKQQE 600

Query: 601 EELRKLKTKLKSR 614
           EELRK K+KLK R
Sbjct: 601 EELRKFKSKLKFR 602

BLAST of Lcy12g015980 vs. ExPASy TrEMBL
Match: A0A6J1FI62 (protein ANTI-SILENCING 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445984 PE=4 SV=1)

HSP 1 Score: 1010.0 bits (2610), Expect = 4.3e-291
Identity = 526/613 (85.81%), Postives = 551/613 (89.89%), Query Frame = 0

Query: 1   MVDTEEVENVEFQWGKRKSIGGKKKDVTFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK 60
           MV+TE+ E VEFQWGKRK IGGKKKDV+FYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK
Sbjct: 1   MVETEQEEKVEFQWGKRKGIGGKKKDVSFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK 60

Query: 61  ILKIWQNHDKTKKVKILWFFRPCEILNYLGAEDTRDNELFLASGDGVGLANVNPLEVIAG 120
           ILKIWQN DKTKKVKILWFFRPCEILNYLGAEDTRDNELFLASGDGVGLANVN LEVIAG
Sbjct: 61  ILKIWQNPDKTKKVKILWFFRPCEILNYLGAEDTRDNELFLASGDGVGLANVNSLEVIAG 120

Query: 121 KCSVLCISNDSRNSRPSDEALKKADFVFCRTFDVGKQEVCNEICEKIAGVEVKFLLNKVD 180
           KCSVLC SNDSRN RPSDEALKKADFVFCRTFDVGK EVCNEICEKIAGVEVKFLLNKVD
Sbjct: 121 KCSVLCYSNDSRNPRPSDEALKKADFVFCRTFDVGKLEVCNEICEKIAGVEVKFLLNKVD 180

Query: 181 TSKDVKRTDKDGKDASGIATVNTESEDPSGQNISNGELTLKPDESSVKKSTEENVDLKVS 240
           TSKDVKR +KD       A VNTESED SG N SNGE TLK ++S+V+KSTE+NVDLK S
Sbjct: 181 TSKDVKRAEKD-------AIVNTESEDLSGWNTSNGESTLKTNDSTVEKSTEDNVDLKAS 240

Query: 241 LPIEKSSNEEKSGACASDGGNGLANTSSKQENILDDKVSPKLKIDSNETPGKDVKALAKD 300
           L IEKSSNEEKSG C S+GGNG+ANTSSK +NILDDKVS KLKID N+TPGKD  AL KD
Sbjct: 241 LSIEKSSNEEKSGVCVSNGGNGMANTSSKHKNILDDKVSSKLKIDYNKTPGKD--ALPKD 300

Query: 301 DEGRVKNPRDSAEVEHRPAKKAKLDSSVQLSHGKTKSDIQKLGLNRNNGDTLASSPKVLV 360
            EGRVK+PRDS+EVEHRPAKKAKLDS V LS GKTK+D QK GLNR+NGD  ASSPK+ V
Sbjct: 301 VEGRVKSPRDSSEVEHRPAKKAKLDSFVHLSLGKTKNDNQKPGLNRSNGDMWASSPKIHV 360

Query: 361 SEDASRTKHVKDSQGTKDSLIKKPKLDEKPTKVSNGKNLKVSSQISSVTDCKIDGQIVEV 420
           SEDAS TK+VKDS GTKDSLIKKPKLDEKPTKVS GKNLK      SV DCKIDGQIVEV
Sbjct: 361 SEDASMTKNVKDSHGTKDSLIKKPKLDEKPTKVSIGKNLK-----PSVADCKIDGQIVEV 420

Query: 421 TRRPDADRSRWFKGLPWEERMKNAHEQGTLVLIQNLDPGYTSGEVEDIVWHAFNESCTAK 480
           TRRPDADRSRWFKGLPWEERMKNAHEQGTLVLIQN DP YTSGEVEDIVWHAFNESCTAK
Sbjct: 421 TRRPDADRSRWFKGLPWEERMKNAHEQGTLVLIQNFDPAYTSGEVEDIVWHAFNESCTAK 480

Query: 481 MIQRTAISMPHIGQAYVVFKTKEAAEKVVRKLDEGCLLLANGSALVGCFATPHLPSKKQT 540
           MIQRTA SMPHIG+AY+VFKTKEAAEKVVRKLDEGCLLLANG+ALVG FATPHL  KKQT
Sbjct: 481 MIQRTANSMPHIGRAYIVFKTKEAAEKVVRKLDEGCLLLANGNALVGSFATPHLQLKKQT 540

Query: 541 FFGHHSIDRLRHQMQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSELACKKLFKQQE 600
           +FGHHSID+LRH MQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSEL  KKLFKQQE
Sbjct: 541 YFGHHSIDKLRHLMQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSELTWKKLFKQQE 599

Query: 601 EELRKLKTKLKSR 614
           EELRK K+KLK R
Sbjct: 601 EELRKFKSKLKFR 599

BLAST of Lcy12g015980 vs. ExPASy TrEMBL
Match: A0A6J1FAD3 (protein ANTI-SILENCING 1-like OS=Cucurbita moschata OX=3662 GN=LOC111443544 PE=4 SV=1)

HSP 1 Score: 988.4 bits (2554), Expect = 1.3e-284
Identity = 512/613 (83.52%), Postives = 548/613 (89.40%), Query Frame = 0

Query: 1   MVDTEEVENVEFQWGKRKSIGGKKKDVTFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK 60
           MV+ E++EN+EFQWGKRK IGGKKKDVTFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK
Sbjct: 1   MVEIEQLENIEFQWGKRKGIGGKKKDVTFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK 60

Query: 61  ILKIWQNHDKTKKVKILWFFRPCEILNYLGAEDTRDNELFLASGDGVGLANVNPLEVIAG 120
           ILKIWQN DKTKKVKILWFFR CEI NYLGAEDTR++ELFLASGDGVGLAN+NPLE IAG
Sbjct: 61  ILKIWQNPDKTKKVKILWFFRSCEIRNYLGAEDTREDELFLASGDGVGLANINPLEAIAG 120

Query: 121 KCSVLCISNDSRNSRPSDEALKKADFVFCRTFDVGKQEVCNEICEKIAGVEVKFLLNKVD 180
           KC+V+CIS+DSRN RPSDE L+KADFVF RTFDVGKQEVCNE+C KIAGVEVKFLLN++D
Sbjct: 121 KCNVICISDDSRNPRPSDEELEKADFVFFRTFDVGKQEVCNEMCAKIAGVEVKFLLNRLD 180

Query: 181 TSKDVKRTDKDGKDASGIATVNTESEDPSGQNISNGELTLKPDESSVKKSTEENVDLKVS 240
           TSKDV RTD DGKDAS IA VNTESEDPSG+N S+GELTL+  E+S K  T+ NV+L+  
Sbjct: 181 TSKDVTRTDTDGKDASVIAIVNTESEDPSGRNTSSGELTLETTENSFKTPTKANVELEE- 240

Query: 241 LPIEKSSNEEKSGACASDGGNGLANTSSKQENILDDKVSPKLKIDSNETPGKDVKALAKD 300
            PIEKSSN E+SG C  DGGNG+A TSSKQENILDDKVS K +IDSNE  GK V+A AKD
Sbjct: 241 -PIEKSSNGERSGVCVIDGGNGMARTSSKQENILDDKVSSKPEIDSNEIHGKGVEARAKD 300

Query: 301 DEGRVKNPRDSAEVEHRPAKKAKLDSSVQLSHGKTKSDIQKLGLNRNNGDTLASSPKVLV 360
            +GRVK+PR SAE EHR AKKAKLDSSVQLSHGKTKSDI+ L LN  NGDTLAS PKVLV
Sbjct: 301 ADGRVKSPRASAEDEHRSAKKAKLDSSVQLSHGKTKSDIEGLALNCKNGDTLASPPKVLV 360

Query: 361 SEDASRTKHVKDSQGTKDSLIKKPKLDEKPTKVSNGKNLKVSSQISSVTDCKIDGQIVEV 420
           SE        KD   TKDSLIKKPKLDEKPTKVSNGKNLK SSQISSVTDCKIDG+IVEV
Sbjct: 361 SE-------TKDYHETKDSLIKKPKLDEKPTKVSNGKNLKASSQISSVTDCKIDGEIVEV 420

Query: 421 TRRPDADRSRWFKGLPWEERMKNAHEQGTLVLIQNLDPGYTSGEVEDIVWHAFNESCTAK 480
           TRRPDADRSRWFKGLPWEER+K+A+EQGTLVLIQNLDP YTSGEVEDIVWHAFNESCTAK
Sbjct: 421 TRRPDADRSRWFKGLPWEERIKDAYEQGTLVLIQNLDPAYTSGEVEDIVWHAFNESCTAK 480

Query: 481 MIQRTAISMPHIGQAYVVFKTKEAAEKVVRKLDEGCLLLANGSALVGCFATPHLPSKKQT 540
           MIQRTAISMPHIGQA+VVFKTKEAAEKVVRKLDEGCLLLANG ALVG FA P LPSKKQ 
Sbjct: 481 MIQRTAISMPHIGQAHVVFKTKEAAEKVVRKLDEGCLLLANGRALVGSFAIPQLPSKKQI 540

Query: 541 FFGHHSIDRLRHQMQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSELACKKLFKQQE 600
           FFGHHSID+LRH MQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSE AC+KLFKQQE
Sbjct: 541 FFGHHSIDKLRHLMQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSERACRKLFKQQE 600

Query: 601 EELRKLKTKLKSR 614
           EELR+LK KLKSR
Sbjct: 601 EELRELKGKLKSR 604

BLAST of Lcy12g015980 vs. ExPASy TrEMBL
Match: A0A1S3CS61 (uncharacterized protein LOC103503724 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503724 PE=4 SV=1)

HSP 1 Score: 984.9 bits (2545), Expect = 1.5e-283
Identity = 509/615 (82.76%), Postives = 552/615 (89.76%), Query Frame = 0

Query: 1   MVDTEEVENVEFQWGKRKSIGGKKKDVTFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK 60
           MV+TE+VENVEFQWGK+K IGGKKKDVTFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK
Sbjct: 1   MVETEQVENVEFQWGKKKGIGGKKKDVTFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK 60

Query: 61  ILKIWQNHDKTKKVKILWFFRPCEILNYLGAEDTRDNELFLASGDGVGLANVNPLEVIAG 120
           +LKIWQN DKTKKVKILWFFR CEILNYLG  DTR+NELFLASGDG+GLANVN LEV+AG
Sbjct: 61  LLKIWQNPDKTKKVKILWFFRSCEILNYLGGVDTRENELFLASGDGIGLANVNSLEVLAG 120

Query: 121 KCSVLCISNDSRNSRPSDEALKKADFVFCRTFDVGKQEVCNEICEKIAGVEVKFLLNKVD 180
           KC+VLCISNDSRN +PSDEALKKADFVFCRTFDVGKQEVC+EIC+KIAGVEVK LLNK D
Sbjct: 121 KCNVLCISNDSRNPQPSDEALKKADFVFCRTFDVGKQEVCSEICDKIAGVEVKLLLNKAD 180

Query: 181 TSKDVKRTDKDGKDASGIATVNTESEDPSGQNISNGELTLKPDESSVKKSTEENVDLKVS 240
           TSKDVKRTDKDGKDASGIA V TE EDPSG++ISNG+LT+  ++SS++K+T+ENVDLK S
Sbjct: 181 TSKDVKRTDKDGKDASGIAIVKTELEDPSGRDISNGKLTVTTNDSSLEKATKENVDLKGS 240

Query: 241 LPIEKSSNEEKSGACASDGGNGLANTS--SKQENILDDKVSPKLKIDSNETPGKDVKALA 300
             IEKSSNEEKS A A + G G+  TS  SK ENIL DKV P++KIDSNE PG      A
Sbjct: 241 --IEKSSNEEKSSAHAIEAGIGMGKTSSISKHENILGDKVPPRIKIDSNEKPGN-----A 300

Query: 301 KDDEGRVKNPRDSAEVEHRPAKKAKLDSSVQLSHGKTKSDIQKLGLNRNNGDTLASSPKV 360
           KD EGRVK+PR+SAEVEHRP KKAKLDSSVQLS G TK+DI+KLG++ NNGDTLASSPKV
Sbjct: 301 KDVEGRVKSPRESAEVEHRPVKKAKLDSSVQLSPGMTKNDIEKLGIDHNNGDTLASSPKV 360

Query: 361 LVSEDASRTKHVKDSQGTKDSLIKKPKLDEKPTKVSNGKNLKVSSQISSVTDCKIDGQIV 420
           LVSEDASR K+VKDS+ TKDS +KKPKLDEKPTKVSNGKNLK SS         IDG++V
Sbjct: 361 LVSEDASRAKNVKDSRETKDSFLKKPKLDEKPTKVSNGKNLKPSS--------LIDGEVV 420

Query: 421 EVTRRPDADRSRWFKGLPWEERMKNAHEQGTLVLIQNLDPGYTSGEVEDIVWHAFNESCT 480
           EVTRRPDADRSRWFKGLPWEER+K+AHEQGTLVLIQNLDP YTSGEVEDIVWHAFNESCT
Sbjct: 421 EVTRRPDADRSRWFKGLPWEERIKDAHEQGTLVLIQNLDPSYTSGEVEDIVWHAFNESCT 480

Query: 481 AKMIQRTAISMPHIGQAYVVFKTKEAAEKVVRKLDEGCLLLANGSALVGCFATPHLPSKK 540
           AKMIQRTA SMPHIGQAYVVFKTKEAAEKVVRKL EGCLLLA+GS LVG F TPHL SKK
Sbjct: 481 AKMIQRTATSMPHIGQAYVVFKTKEAAEKVVRKLHEGCLLLADGSVLVGSFETPHLSSKK 540

Query: 541 QTFFGHHSIDRLRHQMQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSELACKKLFKQ 600
           QTFFGHH ID+LRHQMQREMKEAVSTSHCSQPNT+EYDMAMEWCLLQERSEL CK+LFKQ
Sbjct: 541 QTFFGHHCIDKLRHQMQREMKEAVSTSHCSQPNTIEYDMAMEWCLLQERSELVCKQLFKQ 600

Query: 601 QEEELRKLKTKLKSR 614
           QEEELRKLK+KLKSR
Sbjct: 601 QEEELRKLKSKLKSR 600

BLAST of Lcy12g015980 vs. NCBI nr
Match: XP_038898911.1 (protein ANTI-SILENCING 1 [Benincasa hispida] >XP_038898912.1 protein ANTI-SILENCING 1 [Benincasa hispida])

HSP 1 Score: 1028.5 bits (2658), Expect = 2.4e-296
Identity = 527/613 (85.97%), Postives = 562/613 (91.68%), Query Frame = 0

Query: 1   MVDTEEVENVEFQWGKRKSIGGKKKDVTFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK 60
           MV+TE+VENVEFQWGK+K IGGKKKDVTFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK
Sbjct: 1   MVETEQVENVEFQWGKKKGIGGKKKDVTFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK 60

Query: 61  ILKIWQNHDKTKKVKILWFFRPCEILNYLGAEDTRDNELFLASGDGVGLANVNPLEVIAG 120
           ++KIWQN DKTKKVKILWFFR CEILNYLGAEDTRDNELFLASGDGVGLANVN LEVIAG
Sbjct: 61  LVKIWQNPDKTKKVKILWFFRSCEILNYLGAEDTRDNELFLASGDGVGLANVNSLEVIAG 120

Query: 121 KCSVLCISNDSRNSRPSDEALKKADFVFCRTFDVGKQEVCNEICEKIAGVEVKFLLNKVD 180
           KC+VLCISNDSRN +PSDEALKKADFVFCRTFDVGK E+CNEIC+KIAGVEVK LLNK+D
Sbjct: 121 KCNVLCISNDSRNPQPSDEALKKADFVFCRTFDVGKLELCNEICDKIAGVEVKLLLNKLD 180

Query: 181 TSKDVKRTDKDGKDASGIATVNTESEDPSGQNISNGELTLKPDESSVKKSTEENVDLKVS 240
           +SKDVKRTDKDGKDASG   VNTE EDPS ++ SNGELTL  +++S +KST+ENVDLK  
Sbjct: 181 SSKDVKRTDKDGKDASGTVIVNTELEDPSARDTSNGELTLTTNDNSPEKSTKENVDLKG- 240

Query: 241 LPIEKSSNEEKSGACASDGGNGLANTSSKQENILDDKVSPKLKIDSNETPGKDVKALAKD 300
            PIEKS NEEKSGA A +GGNG+A T +KQENI+DDK  PKLKID+ E P KDVKALAKD
Sbjct: 241 -PIEKSFNEEKSGAHAIEGGNGMAKTCTKQENIVDDKAPPKLKIDTKEKPSKDVKALAKD 300

Query: 301 DEGRVKNPRDSAEVEHRPAKKAKLDSSVQLSHGKTKSDIQKLGLNRNNGDTLASSPKVLV 360
            EGRVK+PR SAE EHRP KKAKLD SVQLSHGKTKSDIQKLGLNRNNGDTLASSPKVLV
Sbjct: 301 VEGRVKSPRGSAEDEHRPVKKAKLDGSVQLSHGKTKSDIQKLGLNRNNGDTLASSPKVLV 360

Query: 361 SEDASRTKHVKDSQGTKDSLIKKPKLDEKPTKVSNGKNLKVSSQISSVTDCKIDGQIVEV 420
           SEDASR K+VKDS  TKDSLIKKPKLDEKPTK+SNGKNLK SSQ        IDG++VEV
Sbjct: 361 SEDASRGKNVKDSHETKDSLIKKPKLDEKPTKISNGKNLKASSQ--------IDGEVVEV 420

Query: 421 TRRPDADRSRWFKGLPWEERMKNAHEQGTLVLIQNLDPGYTSGEVEDIVWHAFNESCTAK 480
           TRRPDADRSRWFKGLPWEER+K+AHEQGTLVLIQNLDP YTSGEVEDIVWHAFNESCTAK
Sbjct: 421 TRRPDADRSRWFKGLPWEERIKDAHEQGTLVLIQNLDPAYTSGEVEDIVWHAFNESCTAK 480

Query: 481 MIQRTAISMPHIGQAYVVFKTKEAAEKVVRKLDEGCLLLANGSALVGCFATPHLPSKKQT 540
           MIQRTAISMPHIGQAYVVFKTKEAAEKVVRKLDEGCLLL+NGS LVG FA+PHL SKKQT
Sbjct: 481 MIQRTAISMPHIGQAYVVFKTKEAAEKVVRKLDEGCLLLSNGSVLVGSFASPHLLSKKQT 540

Query: 541 FFGHHSIDRLRHQMQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSELACKKLFKQQE 600
           FFGHHSID+LRHQMQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSEL CKKLFKQQE
Sbjct: 541 FFGHHSIDKLRHQMQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSELVCKKLFKQQE 600

Query: 601 EELRKLKTKLKSR 614
           EELRKL++KLKSR
Sbjct: 601 EELRKLQSKLKSR 603

BLAST of Lcy12g015980 vs. NCBI nr
Match: XP_023524125.1 (protein ANTI-SILENCING 1 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023524126.1 protein ANTI-SILENCING 1 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023524127.1 protein ANTI-SILENCING 1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1015.4 bits (2624), Expect = 2.1e-292
Identity = 528/613 (86.13%), Postives = 554/613 (90.38%), Query Frame = 0

Query: 1   MVDTEEVENVEFQWGKRKSIGGKKKDVTFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK 60
           MV+TE+ E VEFQWGKRK IGGKKKDV+FYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK
Sbjct: 1   MVETEQEEKVEFQWGKRKGIGGKKKDVSFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK 60

Query: 61  ILKIWQNHDKTKKVKILWFFRPCEILNYLGAEDTRDNELFLASGDGVGLANVNPLEVIAG 120
           ILKIWQN DKTKKVKILWFFRPCEILNYLGAEDTRDNELFLASGDGVGLANVN LEVIAG
Sbjct: 61  ILKIWQNPDKTKKVKILWFFRPCEILNYLGAEDTRDNELFLASGDGVGLANVNSLEVIAG 120

Query: 121 KCSVLCISNDSRNSRPSDEALKKADFVFCRTFDVGKQEVCNEICEKIAGVEVKFLLNKVD 180
           KCSVLC SNDSRN +PSDEALKKADFVFCRTFDVGK EVCNEICEKIAGVEVKFLLNKVD
Sbjct: 121 KCSVLCSSNDSRNPQPSDEALKKADFVFCRTFDVGKLEVCNEICEKIAGVEVKFLLNKVD 180

Query: 181 TSKDVKRTDKDGKDASGIATVNTESEDPSGQNISNGELTLKPDESSVKKSTEENVDLKVS 240
           TSKDVKRT+KDGKD    A VNTESED   +N SNGE TLK ++S+V+ ST+ENVDLK S
Sbjct: 181 TSKDVKRTEKDGKD----AIVNTESEDLFERNTSNGESTLKTNDSTVEMSTKENVDLKAS 240

Query: 241 LPIEKSSNEEKSGACASDGGNGLANTSSKQENILDDKVSPKLKIDSNETPGKDVKALAKD 300
           L IEKSSNEEKSG C S+GGNG+AN SSK +NILDDKVS KLKID N  P KD  AL KD
Sbjct: 241 LSIEKSSNEEKSGVCVSNGGNGMANISSKHKNILDDKVSSKLKIDYNNKPSKD--ALPKD 300

Query: 301 DEGRVKNPRDSAEVEHRPAKKAKLDSSVQLSHGKTKSDIQKLGLNRNNGDTLASSPKVLV 360
            EGRVK+PRDS+E+EHRPAKKAKLDS VQLS GKTK+DIQK GLNR+NGDTLASSPK+ V
Sbjct: 301 VEGRVKSPRDSSEIEHRPAKKAKLDSFVQLSLGKTKNDIQKPGLNRSNGDTLASSPKIHV 360

Query: 361 SEDASRTKHVKDSQGTKDSLIKKPKLDEKPTKVSNGKNLKVSSQISSVTDCKIDGQIVEV 420
           SEDASRTK+VKDS GTKDS+IKKPKLDEKPTKVS GKNLK      SV DCKIDGQIVEV
Sbjct: 361 SEDASRTKNVKDSHGTKDSIIKKPKLDEKPTKVSIGKNLK-----PSVADCKIDGQIVEV 420

Query: 421 TRRPDADRSRWFKGLPWEERMKNAHEQGTLVLIQNLDPGYTSGEVEDIVWHAFNESCTAK 480
           TRRPDADRSRWFKGLPWEERMKNAHEQGTLVLIQN DP YTSGEVEDIVWHAFNESCTAK
Sbjct: 421 TRRPDADRSRWFKGLPWEERMKNAHEQGTLVLIQNFDPAYTSGEVEDIVWHAFNESCTAK 480

Query: 481 MIQRTAISMPHIGQAYVVFKTKEAAEKVVRKLDEGCLLLANGSALVGCFATPHLPSKKQT 540
           MIQRTA SMPHIGQAYVVFKTKEAAEKVVRKLDEGCLLLANG+ALVG FATPHL  KKQT
Sbjct: 481 MIQRTANSMPHIGQAYVVFKTKEAAEKVVRKLDEGCLLLANGNALVGSFATPHLQLKKQT 540

Query: 541 FFGHHSIDRLRHQMQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSELACKKLFKQQE 600
           +FGHHSID+LRH MQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSEL  KKLFKQQE
Sbjct: 541 YFGHHSIDKLRHLMQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSELTWKKLFKQQE 600

Query: 601 EELRKLKTKLKSR 614
           EELRK K+KLK R
Sbjct: 601 EELRKFKSKLKLR 602

BLAST of Lcy12g015980 vs. NCBI nr
Match: XP_022135099.1 (protein ANTI-SILENCING 1 [Momordica charantia])

HSP 1 Score: 1015.0 bits (2623), Expect = 2.7e-292
Identity = 515/615 (83.74%), Postives = 559/615 (90.89%), Query Frame = 0

Query: 1   MVDTEEVENVEFQWGKRKSIGGKKKDVTFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK 60
           MV+ E+VEN+EF+WGKRK+IGGKKKDVTFYES TYDGVEYFLYDSVYLYKEGEPEPYIGK
Sbjct: 1   MVEPEQVENIEFRWGKRKAIGGKKKDVTFYESCTYDGVEYFLYDSVYLYKEGEPEPYIGK 60

Query: 61  ILKIWQNHDKTKKVKILWFFRPCEILNYLGAEDTRDNELFLASGDGVGLANVNPLEVIAG 120
           ILK+WQN DKTKKVKILWFFRPCEILNYLGAEDT DNELFLASG+GVGLANVNPLEVIAG
Sbjct: 61  ILKMWQNPDKTKKVKILWFFRPCEILNYLGAEDTPDNELFLASGEGVGLANVNPLEVIAG 120

Query: 121 KCSVLCISNDSRNSRPSDEALKKADFVFCRTFDVGKQEVCNEICEKIAGVEVKFLLNKVD 180
           KC+VLCISNDSRN RPSDE LKKADFVF RTFDVGKQEVCN++C KIAG+EVKFLLN+VD
Sbjct: 121 KCNVLCISNDSRNPRPSDEVLKKADFVFSRTFDVGKQEVCNDMCGKIAGIEVKFLLNRVD 180

Query: 181 TSKDVKRTDKDGKDASGIATVNTESEDPSGQNISNGELTLKPDESSVKKSTEENVDLKVS 240
           +SKDV RTDKDGKDASG   VNTE +DPS QN  NGELTL+  ESSV+KSTEENVDLKVS
Sbjct: 181 SSKDVNRTDKDGKDASGGTIVNTEFDDPSQQNTPNGELTLEKHESSVEKSTEENVDLKVS 240

Query: 241 LPIEKSSNEEKSGACASDGGNGLANTSSKQENILDDKVSPKLKIDSNETPGKDVKALAKD 300
           LP +KSSNEEKS ACASDGG+G+ANTSSK+ENIL DKVSP+LKID NE  GKDVKALAKD
Sbjct: 241 LPTQKSSNEEKSTACASDGGHGMANTSSKEENILGDKVSPRLKIDPNEIAGKDVKALAKD 300

Query: 301 --DEGRVKNPRDSAEVEHRPAKKAKLDSSVQLSHGKTKSDIQKLGLNRNNGDTLASSPKV 360
              E RV++P+ SAEVEHRPAK+AKLD+S+Q S GK K+++QK GLNRNN DT AS+PK 
Sbjct: 301 VEFEDRVRSPKGSAEVEHRPAKRAKLDTSIQSSPGKAKNNVQKFGLNRNNSDTQASAPKS 360

Query: 361 LVSEDASRTKHVKDSQGTKDSLIKKPKLDEKPTKVSNGKNLKVSSQISSVTDCKIDGQIV 420
           +VSEDAS+TK+VKD  GTKD LIKKPKLDEKP KV NG N+K  SQISS    KIDGQ V
Sbjct: 361 IVSEDASKTKNVKDYHGTKDGLIKKPKLDEKPPKVFNGTNIKSPSQISSDKSAKIDGQTV 420

Query: 421 EVTRRPDADRSRWFKGLPWEERMKNAHEQGTLVLIQNLDPGYTSGEVEDIVWHAFNESCT 480
           EVTRRPDADRSRWFKGLPWEERMK AHEQGTLVLIQNLDP YTSGEVEDIVWHAF+ESCT
Sbjct: 421 EVTRRPDADRSRWFKGLPWEERMKTAHEQGTLVLIQNLDPAYTSGEVEDIVWHAFSESCT 480

Query: 481 AKMIQRTAISMPHIGQAYVVFKTKEAAEKVVRKLDEGCLLLANGSALVGCFATPHLPSKK 540
           AKM+QRTA SMPHIGQAYVVFKTKEAAEKVVRKLDEGCL+L+NG ALVG FATPH P+KK
Sbjct: 481 AKMVQRTAYSMPHIGQAYVVFKTKEAAEKVVRKLDEGCLVLSNGRALVGSFATPHFPAKK 540

Query: 541 QTFFGHHSIDRLRHQMQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSELACKKLFKQ 600
           QTFFGHHSID+LRHQMQREMK+AVSTSHCSQPNTVEYDMAMEWCLL+ERSELACKKLFKQ
Sbjct: 541 QTFFGHHSIDKLRHQMQREMKDAVSTSHCSQPNTVEYDMAMEWCLLEERSELACKKLFKQ 600

Query: 601 QEEELRKLKTKLKSR 614
           QEEELRKLK+KL SR
Sbjct: 601 QEEELRKLKSKLISR 615

BLAST of Lcy12g015980 vs. NCBI nr
Match: XP_022982080.1 (protein ANTI-SILENCING 1-like isoform X1 [Cucurbita maxima] >XP_022982081.1 protein ANTI-SILENCING 1-like isoform X1 [Cucurbita maxima] >XP_022982082.1 protein ANTI-SILENCING 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1012.3 bits (2616), Expect = 1.8e-291
Identity = 528/613 (86.13%), Postives = 552/613 (90.05%), Query Frame = 0

Query: 1   MVDTEEVENVEFQWGKRKSIGGKKKDVTFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK 60
           MV+TE+ E VEFQWGKRK IGGKKKDV+FYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK
Sbjct: 1   MVETEQEEKVEFQWGKRKGIGGKKKDVSFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK 60

Query: 61  ILKIWQNHDKTKKVKILWFFRPCEILNYLGAEDTRDNELFLASGDGVGLANVNPLEVIAG 120
           ILKIWQN DKTKKVKILWFFRPCEILNYLGAEDTRDNELFLASGDGVGLANVN LEVIAG
Sbjct: 61  ILKIWQNPDKTKKVKILWFFRPCEILNYLGAEDTRDNELFLASGDGVGLANVNSLEVIAG 120

Query: 121 KCSVLCISNDSRNSRPSDEALKKADFVFCRTFDVGKQEVCNEICEKIAGVEVKFLLNKVD 180
           KCSVLC SNDSRN RPSDEALKKADFVFCRTFDVGK EVCNEICEKIAGVEVKFLLNKVD
Sbjct: 121 KCSVLCYSNDSRNPRPSDEALKKADFVFCRTFDVGKLEVCNEICEKIAGVEVKFLLNKVD 180

Query: 181 TSKDVKRTDKDGKDASGIATVNTESEDPSGQNISNGELTLKPDESSVKKSTEENVDLKVS 240
           TSK VKR +KDGKD    A VNTESED   +N SNGE TLK  +S+V+ ST+ENVDLK S
Sbjct: 181 TSKYVKRAEKDGKD----AIVNTESEDLFERNTSNGESTLKTKDSTVEMSTKENVDLKAS 240

Query: 241 LPIEKSSNEEKSGACASDGGNGLANTSSKQENILDDKVSPKLKIDSNETPGKDVKALAKD 300
           L IEKSSNEEKSG C SDGGNG+ANTSSK +NILDDKVS KLKID N+TPGKD  AL KD
Sbjct: 241 LSIEKSSNEEKSGVCVSDGGNGMANTSSKHKNILDDKVSSKLKIDYNKTPGKD--ALPKD 300

Query: 301 DEGRVKNPRDSAEVEHRPAKKAKLDSSVQLSHGKTKSDIQKLGLNRNNGDTLASSPKVLV 360
            EGRVK+PRDS+EVEHRP KKAKLDS VQLS GKTKSDIQK GLNR+NGDTLASSPK+ V
Sbjct: 301 VEGRVKSPRDSSEVEHRPEKKAKLDSFVQLSLGKTKSDIQKPGLNRSNGDTLASSPKIHV 360

Query: 361 SEDASRTKHVKDSQGTKDSLIKKPKLDEKPTKVSNGKNLKVSSQISSVTDCKIDGQIVEV 420
           SE+ASRTK+VKDS GTKDSLIKKPKLDEKPTKVS GKNLK      SV DCKIDGQIVEV
Sbjct: 361 SENASRTKNVKDSHGTKDSLIKKPKLDEKPTKVSIGKNLK-----PSVADCKIDGQIVEV 420

Query: 421 TRRPDADRSRWFKGLPWEERMKNAHEQGTLVLIQNLDPGYTSGEVEDIVWHAFNESCTAK 480
           TRRPDADRSRWFKGLPWEERMKNAHEQ TLVLIQN DP YTSGEVEDIVWHAFNESCTAK
Sbjct: 421 TRRPDADRSRWFKGLPWEERMKNAHEQRTLVLIQNFDPAYTSGEVEDIVWHAFNESCTAK 480

Query: 481 MIQRTAISMPHIGQAYVVFKTKEAAEKVVRKLDEGCLLLANGSALVGCFATPHLPSKKQT 540
           MIQRTA SMPHIGQAY+VFKTKEAAEKVVRKLDEGCLLLANG+AL G FATPHL  KKQT
Sbjct: 481 MIQRTANSMPHIGQAYIVFKTKEAAEKVVRKLDEGCLLLANGNALFGSFATPHLQLKKQT 540

Query: 541 FFGHHSIDRLRHQMQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSELACKKLFKQQE 600
           +FGHHSID+LRH MQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERS+L  KKLFKQQE
Sbjct: 541 YFGHHSIDKLRHLMQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSQLTWKKLFKQQE 600

Query: 601 EELRKLKTKLKSR 614
           EELRK K+KLK R
Sbjct: 601 EELRKFKSKLKFR 602

BLAST of Lcy12g015980 vs. NCBI nr
Match: XP_022940335.1 (protein ANTI-SILENCING 1 isoform X1 [Cucurbita moschata] >XP_022940336.1 protein ANTI-SILENCING 1 isoform X1 [Cucurbita moschata] >XP_022940337.1 protein ANTI-SILENCING 1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1010.0 bits (2610), Expect = 8.8e-291
Identity = 526/613 (85.81%), Postives = 551/613 (89.89%), Query Frame = 0

Query: 1   MVDTEEVENVEFQWGKRKSIGGKKKDVTFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK 60
           MV+TE+ E VEFQWGKRK IGGKKKDV+FYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK
Sbjct: 1   MVETEQEEKVEFQWGKRKGIGGKKKDVSFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGK 60

Query: 61  ILKIWQNHDKTKKVKILWFFRPCEILNYLGAEDTRDNELFLASGDGVGLANVNPLEVIAG 120
           ILKIWQN DKTKKVKILWFFRPCEILNYLGAEDTRDNELFLASGDGVGLANVN LEVIAG
Sbjct: 61  ILKIWQNPDKTKKVKILWFFRPCEILNYLGAEDTRDNELFLASGDGVGLANVNSLEVIAG 120

Query: 121 KCSVLCISNDSRNSRPSDEALKKADFVFCRTFDVGKQEVCNEICEKIAGVEVKFLLNKVD 180
           KCSVLC SNDSRN RPSDEALKKADFVFCRTFDVGK EVCNEICEKIAGVEVKFLLNKVD
Sbjct: 121 KCSVLCYSNDSRNPRPSDEALKKADFVFCRTFDVGKLEVCNEICEKIAGVEVKFLLNKVD 180

Query: 181 TSKDVKRTDKDGKDASGIATVNTESEDPSGQNISNGELTLKPDESSVKKSTEENVDLKVS 240
           TSKDVKR +KD       A VNTESED SG N SNGE TLK ++S+V+KSTE+NVDLK S
Sbjct: 181 TSKDVKRAEKD-------AIVNTESEDLSGWNTSNGESTLKTNDSTVEKSTEDNVDLKAS 240

Query: 241 LPIEKSSNEEKSGACASDGGNGLANTSSKQENILDDKVSPKLKIDSNETPGKDVKALAKD 300
           L IEKSSNEEKSG C S+GGNG+ANTSSK +NILDDKVS KLKID N+TPGKD  AL KD
Sbjct: 241 LSIEKSSNEEKSGVCVSNGGNGMANTSSKHKNILDDKVSSKLKIDYNKTPGKD--ALPKD 300

Query: 301 DEGRVKNPRDSAEVEHRPAKKAKLDSSVQLSHGKTKSDIQKLGLNRNNGDTLASSPKVLV 360
            EGRVK+PRDS+EVEHRPAKKAKLDS V LS GKTK+D QK GLNR+NGD  ASSPK+ V
Sbjct: 301 VEGRVKSPRDSSEVEHRPAKKAKLDSFVHLSLGKTKNDNQKPGLNRSNGDMWASSPKIHV 360

Query: 361 SEDASRTKHVKDSQGTKDSLIKKPKLDEKPTKVSNGKNLKVSSQISSVTDCKIDGQIVEV 420
           SEDAS TK+VKDS GTKDSLIKKPKLDEKPTKVS GKNLK      SV DCKIDGQIVEV
Sbjct: 361 SEDASMTKNVKDSHGTKDSLIKKPKLDEKPTKVSIGKNLK-----PSVADCKIDGQIVEV 420

Query: 421 TRRPDADRSRWFKGLPWEERMKNAHEQGTLVLIQNLDPGYTSGEVEDIVWHAFNESCTAK 480
           TRRPDADRSRWFKGLPWEERMKNAHEQGTLVLIQN DP YTSGEVEDIVWHAFNESCTAK
Sbjct: 421 TRRPDADRSRWFKGLPWEERMKNAHEQGTLVLIQNFDPAYTSGEVEDIVWHAFNESCTAK 480

Query: 481 MIQRTAISMPHIGQAYVVFKTKEAAEKVVRKLDEGCLLLANGSALVGCFATPHLPSKKQT 540
           MIQRTA SMPHIG+AY+VFKTKEAAEKVVRKLDEGCLLLANG+ALVG FATPHL  KKQT
Sbjct: 481 MIQRTANSMPHIGRAYIVFKTKEAAEKVVRKLDEGCLLLANGNALVGSFATPHLQLKKQT 540

Query: 541 FFGHHSIDRLRHQMQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSELACKKLFKQQE 600
           +FGHHSID+LRH MQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSEL  KKLFKQQE
Sbjct: 541 YFGHHSIDKLRHLMQREMKEAVSTSHCSQPNTVEYDMAMEWCLLQERSELTWKKLFKQQE 599

Query: 601 EELRKLKTKLKSR 614
           EELRK K+KLK R
Sbjct: 601 EELRKFKSKLKFR 599

BLAST of Lcy12g015980 vs. TAIR 10
Match: AT5G11470.1 (bromo-adjacent homology (BAH) domain-containing protein )

HSP 1 Score: 397.9 bits (1021), Expect = 1.5e-110
Identity = 274/705 (38.87%), Postives = 379/705 (53.76%), Query Frame = 0

Query: 1   MVDTEEVENVEFQWGKRKSIGGKKKDVTFYESFTYDGVEYFLYDSVYLYKEGEP---EPY 60
           M ++   E +EF+WGK+K +GGKKKDV FYESFTYDG EY LYD V +    EP   EP+
Sbjct: 1   MEESVASEGLEFKWGKKKGVGGKKKDVQFYESFTYDGDEYRLYDCVLVGNASEPDSTEPF 60

Query: 61  IGKILKIWQNHDK--TKKVKILWFFRPCEILNYL-GAEDTRDNELFLASGDGVGLANVNP 120
           IG I+KIW++ +K   KKVK+LWFF+P EI  YL G  +   NE+FLASG+G+GLAN N 
Sbjct: 61  IGMIIKIWEHANKHIPKKVKLLWFFKPSEIAPYLEGVPNVLANEVFLASGEGLGLANTNQ 120

Query: 121 LEVIAGKCSVLCISNDSRNSRPSDEALKKADFVFCRTFDVGKQEVCNEICEKIAGVEVKF 180
           LE I GKCSVLCIS D RN +PSDE    ADFVFCR FDVG  +V + I +KIAGV+VKF
Sbjct: 121 LEAIGGKCSVLCISKDKRNPQPSDEKFNSADFVFCRAFDVGSCKVVDTIDDKIAGVDVKF 180

Query: 181 LLNKVDTSKDVKRTDKDGKDASGIATVNTESEDPSGQNISNGELTLKPDESSVKKST--- 240
           + N+  + K+         D +G     ++S  P+G        +++  E S  +S+   
Sbjct: 181 IFNRACSEKEATAVQNIEADVNG----KSDSLKPNGPLARGASGSVRKIEDSAFESSDCK 240

Query: 241 -------EENVDLKVSLPIEKSS-NEEKSGACASDGG---NGLANTSSKQENILDDKVSP 300
                  EE       L I+KS+  EE+S   +   G   NG    S  ++ +   K  P
Sbjct: 241 ENSNGCKEEKEKGHYQLAIKKSTLAEERSNKDSGSRGNHYNGKDQESEVKKQLTKQKSMP 300

Query: 301 KLKIDSNETPGKDVKAL----AKDDEGRVKN--------------PRDSAEVEHRPAKKA 360
             +  SN       + +     K  E  VK                ++S+ ++ RP KK 
Sbjct: 301 GEERYSNSFEASGSRTIHSISKKAQENDVKKQLTKQKSMPAGERYSQESSGLDDRPLKKQ 360

Query: 361 KLDSSVQLSHGKTKSDIQKLGLN--RNNGDTLASSPKVLVSE--------DASRTKHVKD 420
           KLD SV +  G   + +Q +  +  ++ G       KV + E          +R   V  
Sbjct: 361 KLDGSVTVRDGWDTTILQNITSDGKKDTGSFKRPRDKVTIEEVPPEKRSFVKNRDLVVSV 420

Query: 421 SQGTKDSLIKKPKLDEKPTKVSNGKNLKVSSQISSVTDCKIDGQIVEVTRRPDA------ 480
           S+G     + +  + +KP   S G+    +    S  D + + Q+ EV RRPDA      
Sbjct: 421 SEGKTTKTVTEKGISKKP---SFGR----AEDKMSADDNERNYQVTEVCRRPDARVVKSL 480

Query: 481 DRSRWF------KGL----------------------------------PWEERMKNAHE 540
           D   WF      KG                                   PWEE M+ A +
Sbjct: 481 DARMWFCKNTILKGAVLFYSPSHCMLYSCSDVLSFFSSYYVTTMLGNIEPWEESMREAEK 540

Query: 541 QGTLVLIQNLDPGYTSGEVEDIVWHAFNESCTAKMIQRTAISMPHIGQAYVVFKTKEAAE 600
           +GT+VL+QNLDP YTS EVEDIV+ A N+ C A+MI+RT++++PHIG+A V+FKT+E AE
Sbjct: 541 KGTVVLLQNLDPTYTSDEVEDIVYSALNQQCEARMIERTSVTIPHIGEALVIFKTREVAE 600

Query: 601 KVVRKLDEGCLLLANGSALVGCFATPHLPSKKQTFFGHHSIDRLRHQMQREMKEAVSTSH 612
           +V+R+LDEGCLLL++G  LV  FA    P K   F GH  + +   Q +REM++AV+TSH
Sbjct: 601 RVIRRLDEGCLLLSSGRPLVASFAKITPPGKPSLFSGHIKLHKT--QTRREMRDAVATSH 660

BLAST of Lcy12g015980 vs. TAIR 10
Match: AT3G15605.4 (nucleic acid binding )

HSP 1 Score: 181.0 bits (458), Expect = 2.8e-45
Identity = 151/544 (27.76%), Postives = 239/544 (43.93%), Query Frame = 0

Query: 8   ENVEFQWGKRKSIGGKKKDVTFYESFTYDGVEYFLYDSVYLYKEGEPEPYIGKILKIWQN 67
           +N +F+WG ++ +G K   V FYESFT +G+EY L+D  Y Y+                 
Sbjct: 11  DNPDFKWGAKRGVGRKDNKVRFYESFTLEGIEYRLFDCAYFYR----------------- 70

Query: 68  HDKTKKVKILWFFRPCEILNYLGAEDTRDNELFLASGDGVGLANVNPLEVIAGKCSVLCI 127
                               +LG  + + +ELFLA GD  G++N+N +E I GKC+V+C 
Sbjct: 71  --------------------FLGEYEPQWDELFLACGDEKGVSNINDVETIMGKCNVVCT 130

Query: 128 SNDSRNSRPSDEALKKADFVFCRTFDVGKQEVCNEICEKIAGVEVKFLLNKVDTSKDVKR 187
           S+D RN RP  + L++A ++F RTFD   + +  +  + IAG+ V  L N     + VKR
Sbjct: 131 SDDRRNPRPGTKELRRAKYIFSRTFDTRLRIISEDFADAIAGIGVDKLFNMRRDKQPVKR 190

Query: 188 TDKDGKDASGIATVNTESEDPSGQNISNGELTLKPDESSVKKSTEENVDLKVSLPIEKSS 247
            +      S  A +   S   S           +PD  S K    +N D K+        
Sbjct: 191 LN------SSAAAITRASPVKS----------FRPDLGSTKLGKHDNRDAKL-------- 250

Query: 248 NEEKSGACASDGGNGLANTSS-KQENILDDKVSPKLKIDSNETPGKDVKALAKDDEGRVK 307
                          ++ TSS K+   L+D+                             
Sbjct: 251 ---------------MSRTSSLKKVTFLEDRA---------------------------- 310

Query: 308 NPRDSAEVEHRPAKKAKLDSSVQLSHGKTKSDIQKLGLNRNNGDTLASSPKVLVSEDASR 367
              D   V+  P    +  +  Q+   KT          R  G+  AS   V  ++ + +
Sbjct: 311 ---DHVHVKRNPPVNTETTTRGQIPILKT----------RAFGELYASGSSV-NAKPSKK 370

Query: 368 TKHVKDSQGTKDSLIKKPKLDEKPTKVSNGKNLKVSSQISSVTDCKIDGQIVEVTRRPDA 427
            K + ++  T DS  + P+  EK       K +K             +  +VE     + 
Sbjct: 371 RKLILNTPETDDSDDQGPQSGEK-------KLIK-------------NPPLVEKAPSQNI 416

Query: 428 DRSRWFKGLPWEERMKNAHEQGTLVLIQNLDPGYTSGEVEDIVWHAFNESCTAKMIQRTA 487
           ++  W+K LP+E+ +K A E+G ++LI+NL+P YTS EVE +   AF E   AKMI  + 
Sbjct: 431 EKKSWYKKLPFEDELKPAIEKGRVLLIENLEPSYTSLEVEFLFRQAFKEGVDAKMIPSSP 416

Query: 488 ISMPHIGQAYVVFKTKEAAEKVVRKLDEGCLLLANGSALVGCFATPHLPSKKQTFFGH-H 547
           +S PH G+A V+F T +AA+  + +L+E CL+L+   AL G    P    + ++F GH +
Sbjct: 491 MSSPHSGRALVIFGTTKAADSAMSRLNEDCLMLSGQRALTGSKNVPVEIGRCRSFTGHFN 416

Query: 548 SIDR 550
            +DR
Sbjct: 551 MVDR 416

BLAST of Lcy12g015980 vs. TAIR 10
Match: AT3G15605.2 (nucleic acid binding )

HSP 1 Score: 124.0 bits (310), Expect = 4.1e-28
Identity = 117/432 (27.08%), Postives = 187/432 (43.29%), Query Frame = 0

Query: 120 GKCSVLCISNDSRNSRPSDEALKKADFVFCRTFDVGKQEVCNEICEKIAGVEVKFLLNKV 179
           GKC+V+C S+D RN RP  + L++A ++F RTFD   + +  +  + IAG+ V  L N  
Sbjct: 2   GKCNVVCTSDDRRNPRPGTKELRRAKYIFSRTFDTRLRIISEDFADAIAGIGVDKLFNMR 61

Query: 180 DTSKDVKRTDKDGKDASGIATVNTESEDPSGQNISNGELTLKPDESSVKKSTEENVDLKV 239
              + VKR +      S  A +   S   S           +PD  S K    +N D K+
Sbjct: 62  RDKQPVKRLN------SSAAAITRASPVKS----------FRPDLGSTKLGKHDNRDAKL 121

Query: 240 SLPIEKSSNEEKSGACASDGGNGLANTSS-KQENILDDKVSPKLKIDSNETPGKDVKALA 299
                                  ++ TSS K+   L+D+                     
Sbjct: 122 -----------------------MSRTSSLKKVTFLEDRA-------------------- 181

Query: 300 KDDEGRVKNPRDSAEVEHRPAKKAKLDSSVQLSHGKTKSDIQKLGLNRNNGDTLASSPKV 359
                      D   V+  P    +  +  Q+   KT          R  G+  AS   V
Sbjct: 182 -----------DHVHVKRNPPVNTETTTRGQIPILKT----------RAFGELYASGSSV 241

Query: 360 LVSEDASRTKHVKDSQGTKDSLIKKPKLDEKPTKVSNGKNLKVSSQISSVTDCKIDGQIV 419
             ++ + + K + ++  T DS  + P+  EK       K +K             +  +V
Sbjct: 242 -NAKPSKKRKLILNTPETDDSDDQGPQSGEK-------KLIK-------------NPPLV 301

Query: 420 EVTRRPDADRSRWFKGLPWEERMKNAHEQGTLVLIQNLDPGYTSGEVEDIVWHAFNESCT 479
           E     + ++  W+K LP+E+ +K A E+G ++LI+NL+P YTS EVE +   AF E   
Sbjct: 302 EKAPSQNIEKKSWYKKLPFEDELKPAIEKGRVLLIENLEPSYTSLEVEFLFRQAFKEGVD 332

Query: 480 AKMIQRTAISMPHIGQAYVVFKTKEAAEKVVRKLDEGCLLLANGSALVGCFATPHLPSKK 539
           AKMI  + +S PH G+A V+F T +AA+  + +L+E CL+L+   AL G    P    + 
Sbjct: 362 AKMIPSSPMSSPHSGRALVIFGTTKAADSAMSRLNEDCLMLSGQRALTGSKNVPVEIGRC 332

Query: 540 QTFFGH-HSIDR 550
           ++F GH + +DR
Sbjct: 422 RSFTGHFNMVDR 332

BLAST of Lcy12g015980 vs. TAIR 10
Match: AT3G15605.3 (nucleic acid binding )

HSP 1 Score: 124.0 bits (310), Expect = 4.1e-28
Identity = 117/432 (27.08%), Postives = 187/432 (43.29%), Query Frame = 0

Query: 120 GKCSVLCISNDSRNSRPSDEALKKADFVFCRTFDVGKQEVCNEICEKIAGVEVKFLLNKV 179
           GKC+V+C S+D RN RP  + L++A ++F RTFD   + +  +  + IAG+ V  L N  
Sbjct: 2   GKCNVVCTSDDRRNPRPGTKELRRAKYIFSRTFDTRLRIISEDFADAIAGIGVDKLFNMR 61

Query: 180 DTSKDVKRTDKDGKDASGIATVNTESEDPSGQNISNGELTLKPDESSVKKSTEENVDLKV 239
              + VKR +      S  A +   S   S           +PD  S K    +N D K+
Sbjct: 62  RDKQPVKRLN------SSAAAITRASPVKS----------FRPDLGSTKLGKHDNRDAKL 121

Query: 240 SLPIEKSSNEEKSGACASDGGNGLANTSS-KQENILDDKVSPKLKIDSNETPGKDVKALA 299
                                  ++ TSS K+   L+D+                     
Sbjct: 122 -----------------------MSRTSSLKKVTFLEDRA-------------------- 181

Query: 300 KDDEGRVKNPRDSAEVEHRPAKKAKLDSSVQLSHGKTKSDIQKLGLNRNNGDTLASSPKV 359
                      D   V+  P    +  +  Q+   KT          R  G+  AS   V
Sbjct: 182 -----------DHVHVKRNPPVNTETTTRGQIPILKT----------RAFGELYASGSSV 241

Query: 360 LVSEDASRTKHVKDSQGTKDSLIKKPKLDEKPTKVSNGKNLKVSSQISSVTDCKIDGQIV 419
             ++ + + K + ++  T DS  + P+  EK       K +K             +  +V
Sbjct: 242 -NAKPSKKRKLILNTPETDDSDDQGPQSGEK-------KLIK-------------NPPLV 301

Query: 420 EVTRRPDADRSRWFKGLPWEERMKNAHEQGTLVLIQNLDPGYTSGEVEDIVWHAFNESCT 479
           E     + ++  W+K LP+E+ +K A E+G ++LI+NL+P YTS EVE +   AF E   
Sbjct: 302 EKAPSQNIEKKSWYKKLPFEDELKPAIEKGRVLLIENLEPSYTSLEVEFLFRQAFKEGVD 332

Query: 480 AKMIQRTAISMPHIGQAYVVFKTKEAAEKVVRKLDEGCLLLANGSALVGCFATPHLPSKK 539
           AKMI  + +S PH G+A V+F T +AA+  + +L+E CL+L+   AL G    P    + 
Sbjct: 362 AKMIPSSPMSSPHSGRALVIFGTTKAADSAMSRLNEDCLMLSGQRALTGSKNVPVEIGRC 332

Query: 540 QTFFGH-HSIDR 550
           ++F GH + +DR
Sbjct: 422 RSFTGHFNMVDR 332

BLAST of Lcy12g015980 vs. TAIR 10
Match: AT3G15605.1 (nucleic acid binding )

HSP 1 Score: 119.0 bits (297), Expect = 1.3e-26
Identity = 117/434 (26.96%), Postives = 187/434 (43.09%), Query Frame = 0

Query: 120 GKCSVLCISNDSRNSRPSDEALKKADFVFCRTFDVGKQEVCNEICEKIAGVEVKFLLNKV 179
           GKC+V+C S+D RN RP  + L++A ++F RTFD   + +  +  + IAG+ V  L N  
Sbjct: 2   GKCNVVCTSDDRRNPRPGTKELRRAKYIFSRTFDTRLRIISEDFADAIAGIGVDKLFNMR 61

Query: 180 DTSKDVKRTDKDGKDASGIATVNTESEDPSGQNISNGELTLKPDESSVKKSTEENVDLKV 239
              + VKR +      S  A +   S   S           +PD  S K    +N D K+
Sbjct: 62  RDKQPVKRLN------SSAAAITRASPVKS----------FRPDLGSTKLGKHDNRDAKL 121

Query: 240 SLPIEKSSNEEKSGACASDGGNGLANTSS-KQENILDDKVSPKLKIDSNETPGKDVKALA 299
                                  ++ TSS K+   L+D+                     
Sbjct: 122 -----------------------MSRTSSLKKVTFLEDRA-------------------- 181

Query: 300 KDDEGRVKNPRDSAEVEHRPAKKAKLDSSVQLSHGKTKSDIQKLGLNRNNGDTLASSPKV 359
                      D   V+  P    +  +  Q+   KT          R  G+  AS   V
Sbjct: 182 -----------DHVHVKRNPPVNTETTTRGQIPILKT----------RAFGELYASGSSV 241

Query: 360 LVSEDASRTKHVKDSQGTKDSLIKKPKLDEKPTKVSNGKNLKVSSQISSVTDCKIDGQIV 419
             ++ + + K + ++  T DS  + P+  EK       K +K             +  +V
Sbjct: 242 -NAKPSKKRKLILNTPETDDSDDQGPQSGEK-------KLIK-------------NPPLV 301

Query: 420 EVTRRPDADRSRWFKGLPWEERMKNAHEQGTLVLIQNLDPGYTSGEVEDIVWHAFNESCT 479
           E     + ++  W+K LP+E+ +K A E+G ++LI+NL+P YTS EVE +   AF E   
Sbjct: 302 EKAPSQNIEKKSWYKKLPFEDELKPAIEKGRVLLIENLEPSYTSLEVEFLFRQAFKEGVD 334

Query: 480 AKMIQRTAISMPHIG--QAYVVFKTKEAAEKVVRKLDEGCLLLANGSALVGCFATPHLPS 539
           AKMI  + +S PH G  +A V+F T +AA+  + +L+E CL+L+   AL G    P    
Sbjct: 362 AKMIPSSPMSSPHSGICRALVIFGTTKAADSAMSRLNEDCLMLSGQRALTGSKNVPVEIG 334

Query: 540 KKQTFFGH-HSIDR 550
           + ++F GH + +DR
Sbjct: 422 RCRSFTGHFNMVDR 334