Lcy12g013940 (gene) Sponge gourd (P93075) v1

BLAST of Lcy12g013940 vs. ExPASy Swiss-Prot
Match: Q93YU8 (Nitrate regulatory gene2 protein OS=Arabidopsis thaliana OX=3702 GN=NRG2 PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 3.3e-17
Identity = 171/651 (26.27%), Postives = 266/651 (40.86%), Query Frame = 0

Query: 1   MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSLTDIGEAIRKFVD-EE 60
           MGC  SK+D    V  C++R+ L+K A   R+ LAAAH  Y +SL   G A+  F   E 
Sbjct: 1   MGCAASKLDNEDAVRRCKDRRRLMKEAVYARHHLAAAHADYCRSLRITGSALSSFASGEP 60

Query: 61  IVISGADSSSTHGSPVLTLPSDEGKGKRKKPKSGEKHINSS-SSSSILHSVSISHEPSPH 120
           + +S    +    +P   L S++   K   P+       SS    S   SV+ S +PS  
Sbjct: 61  LSVSDQTPAVFLHTPPPPL-SEQSPAKFVPPRFSPSPAPSSVYPPSTSPSVASSKQPSV- 120

Query: 121 EDEIDGSHLHLSSGSDSESEHHSS---GHIQIEDSP---VQDEGYSHPPYAYPPRDWSPP 180
                     +S+ S+   +        HI  E SP    + E  +  P  YP       
Sbjct: 121 ----------MSTSSNRRRKQQPKPRLPHILSESSPSSSPRSERSNFMPNLYPS------ 180

Query: 181 TNTYAYYMQRSTTPAKTVMYDGPETHTASDGQWPDPSYSYPQYPQYENGGFYGFSMGSQP 240
               AY  Q ST       Y    +H +S   W +    YP  P               P
Sbjct: 181 ----AY--QNST-------YSATPSHASSVWNWEN---FYPPSP---------------P 240

Query: 241 DYNLYNQQPRRPATPPPPPSPPKVSAWDFMNVFDGYDNVYQDYNAGNRYGYGSIQSSPDS 300
           D   +N    R A      S  + +  D   V   YD  + D     +  + S+++  + 
Sbjct: 241 DSEFFN----RKAQEKKHNSDNRFNDEDTETVRSEYD--FFDTRKQKQKQFESMRNQVEE 300

Query: 301 NEVREREGI--PELED-------ETEPEALKEIKERKKLNVETMNTNMNSGEGT-SKFVT 360
               ERE +   E ED        +   A +E ++  + ++  + T    G    S  + 
Sbjct: 301 ETETEREEVQCSEWEDHDHYSTTSSSDAAEEEEEDDDRESISEVGTRSEFGSTVRSNSMR 360

Query: 361 PHSSEDSTKSVPLPNSGSSPISKEKGIDNSPDTNVSKKSEQEEPVEKKELSFEIEETSTL 420
            H  + S    P+P       +++   D + D  +S  S +                   
Sbjct: 361 RHHQQPS----PMPQVYGG--AEQSKYDKADDATISSGSYR----------------GGG 420

Query: 421 DIESSKKSNLATFTAYGTRDLQEVVNEIKDEFEAASNHGKEVAMLLEVGRLPYRSKIAVL 480
           DI   K            RDL+E+++ IK+ F+ A+  G++V+ +LE+GR       + L
Sbjct: 421 DIADMK-------MVVRHRDLKEIIDAIKENFDKAAASGEQVSQMLELGRAELDRSFSQL 480

Query: 481 KVILSRIQYLVA--PSSASSQPPLIWLDPKTVKRAKAYSGSGSPGNELDLKSGSLSSTLE 540
           K  +     L++   S+ +S+PPL       VK     +    P +     S SL STL+
Sbjct: 481 KKTVIHSSSLLSNLSSTWTSKPPL------AVKYRIDTTALDQPNS-----SKSLCSTLD 540

Query: 541 KLYVWEKKLYKEVKDEERLRVIYEKLCKKLKRLDDHGADSTKIDATHASIRKLSTKIDIC 600
           +L  WEKKLY+E+K  E  ++ +EK   +L+  +  G D  K+D T ASI +L + I + 
Sbjct: 541 RLLAWEKKLYEEIKAREGFKIEHEKKLSQLQSQEYKGEDEAKLDKTKASITRLQSLIIVT 556

Query: 601 IKAVDAISSRIHKLRDEELQPQLTELIHG--YSNASVLDYSFLLKSIVAMI 630
            +AV   S+ I +LRD +L PQL EL HG  Y   S+  Y     SIV  +
Sbjct: 601 SQAVTTTSTAIIRLRDTDLVPQLVELCHGFMYMWKSMHQYHETQNSIVEQV 556

BLAST of Lcy12g013940 vs. ExPASy Swiss-Prot
Match: A0A178VBJ0 (Protein ALTERED PHOSPHATE STARVATION RESPONSE 1 OS=Arabidopsis thaliana OX=3702 GN=APSR1 PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 2.0e-14
Identity = 96/368 (26.09%), Postives = 148/368 (40.22%), Query Frame = 0

Query: 244 PATPPPPPSPPKVSAWDFMNVFDGYDNVYQDYNAGNRYGYGSIQSSPDSNEVREREGIPE 303
           P  PPPPP PP  S WDF + F                    I   P S+E        E
Sbjct: 106 PPPPPPPPPPPPSSTWDFWDPF--------------------IPPPPSSSE-------EE 165

Query: 304 LEDETEPEALKEIKERKKLNVETMNTNMNSGEGTSKFVTPHSSEDSTKSVPLPNSGSSPI 363
            E+ET                 T  T   +G G+   VT       T + P  +S  S  
Sbjct: 166 WEEET-----------------TTATRTATGTGSDAAVT----TAPTTATPQASSVVSGF 225

Query: 364 SKEKGIDNSPDTNVSKKSEQEEPVEKKELSFEIEETSTLDIESSKKSNLATFTAYGTRDL 423
           SK                                +T T    ++  S LA   +   +DL
Sbjct: 226 SK--------------------------------DTMT---TTTTGSELAVVVSRNGKDL 285

Query: 424 QEVVNEIKDEFEAASNHGKEVAMLLEVGRLPYRSKIAVLKVILSRIQYLVAPSSASSQPP 483
            E++ E+ + F  A++ G  ++ LLE+      S         S   Y  +    +  P 
Sbjct: 286 MEIIKEVDEYFLKAADSGAPLSSLLEIS----TSITDFSGHSKSGKMYSSSNYECNLNPT 345

Query: 484 LIWLDPKTVKRAKAYSGSGS--PGNELDLKSGSLSSTLEKLYVWEKKLYKEVKDEERLRV 543
             W       +   Y  +G    GN +    GS SST+++LY WEKKLY+EVK  E +++
Sbjct: 346 SFWTRGFAPSKLSEYRNAGGVIGGNCI---VGSHSSTVDRLYAWEKKLYQEVKYAESIKM 383

Query: 544 IYEKLCKKLKRLDDHGADSTKIDATHASIRKLSTKIDICIKAVDAISSRIHKLRDEELQP 603
            +EK  ++++RL+   A+  K +     + KL +++ +  +A+ + S+ I KLR+ EL P
Sbjct: 406 DHEKKVEQVRRLEMKRAEYVKTEKAKKDVEKLESQLSVSSQAIQSASNEIIKLRETELYP 383

Query: 604 QLTELIHG 610
           QL EL+ G
Sbjct: 466 QLVELVKG 383

HSP 2 Score: 44.7 bits (104), Expect = 4.7e-03
Identity = 19/59 (32.20%), Postives = 34/59 (57.63%), Query Frame = 0

Query: 1  MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSLTDIGEAIRKFVDEE 60
          MGC  S++D  ++V+ C+ RK  +K   + R  L+ +H  Y +SL  +G ++  F  +E
Sbjct: 1  MGCCQSRIDSKEIVSRCKARKRYLKHLVKARQTLSVSHALYLRSLRAVGSSLVHFSSKE 59

BLAST of Lcy12g013940 vs. ExPASy Swiss-Prot
Match: Q9AQW1 (Protein ROLLING AND ERECT LEAF 2 OS=Oryza sativa subsp. japonica OX=39947 GN=REL2 PE=2 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 1.8e-10
Identity = 151/615 (24.55%), Postives = 228/615 (37.07%), Query Frame = 0

Query: 1   MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSLTDIGEAIRKFVDEEI 60
           MGC  SKV++   V  C+ER+  +K A   R  LA+AH  Y +SL     A+ +F     
Sbjct: 1   MGCTASKVEQEDTVRRCKERRRHMKEAVASRQQLASAHADYLRSLRLTAAALSRFAQGHP 60

Query: 61  VISGADSSSTHGSPVLTLPSDEGKGKRKKPKSGEKHINSSSSSSILHSVSISHEPSPHED 120
            +    + S H +PVL   +         P       +S++SSS+         P P   
Sbjct: 61  SL----AVSHHTAPVLLTTAAPALAPTPTPPPP----SSTASSSL---------PPP--- 120

Query: 121 EIDGSHLHLSSGSDSESEHHSSGHIQIEDSPVQDEGYSHPPYAYPPRDWSPPTNTYAYYM 180
                                        +P+  +    PP   PP    PPT ++    
Sbjct: 121 -----------------------------TPLLPKHQQAPP---PP----PPTQSH---- 180

Query: 181 QRSTTPAKTVMYDGPE----THTASDGQWPDPSYSYPQYPQYENGGFYGFSMGSQPDYNL 240
           Q     A      GP      H  SD     P+ S                         
Sbjct: 181 QPPPPVAVRAPRGGPRRLKVPHILSDSSVASPARS------------------------- 240

Query: 241 YNQQPRRPATPPPPPSPPKVSAWDFMNVFDGYDNVYQDYNAGNRYGYGSIQSSPDSNEVR 300
                R+P    P  S    SAWD       ++N Y      + +         ++N +R
Sbjct: 241 ---SFRKPVVGTPSSS----SAWD-------WENFYPPSPPDSEFFDRRKADLEEANRLR 300

Query: 301 EREGIPELEDETEPEALKEIKERKKLNVETMNTNMNSG---EGTSKFVTPHSSEDSTKSV 360
           E E   +      P  LKE ++    + +     M+ G   +    + +  +SE  ++  
Sbjct: 301 ELEEEEKARGYLHPHHLKE-EDEVDDDDDEREEEMHCGGWEDDDDHYASTTTSETRSEEG 360

Query: 361 PLPNSGSSPISKEKGIDNSPDTNVSKKSEQEEPVEKKELSFEIEETSTLDIESSKKSNLA 420
            + N        E G     +   +  SE         L    E +   D  S+  +   
Sbjct: 361 EMGN------RSECGFAARSEYGGTAPSEYAAAPLPLPLRRRDERSEAGDSSSTVTAAAE 420

Query: 421 TFTAYGTRDLQEVVNEIKDEFEAASNHGKEVAMLLEVGRLPYRSKIAVLKVILSRIQYLV 480
                  R L E+V  I++ F  A+  G  V+ LLE  R         LK  +     L+
Sbjct: 421 MRMVIRHRTLAEIVAAIEEYFVKAAEAGNGVSELLEASRAQLDRNFRQLKKTVYHSNSLL 480

Query: 481 APSSA--SSQPPLIWLDPKTVKRAKAYSGSGSPGNELDLKSGSLSSTLEKLYVWEKKLYK 540
           +  S+  +S+PPL          A  Y    +      ++  S  STLE+L  WEKKLY+
Sbjct: 481 SSLSSTWTSKPPL----------AVRYKLDTNALEMESMEGKSHGSTLERLLAWEKKLYQ 499

Query: 541 EVKDEERLRVIYEKLCKKLKRLDDHGADSTKIDATHASIRKLSTKIDICIKAVDAISSRI 600
           EVK  E +++ +EK    L+ L+  G DSTK+D T ASI KL + I +  +A    SS I
Sbjct: 541 EVKARESVKIEHEKKLSTLQSLEYRGRDSTKLDKTKASINKLQSLIIVTSQAATTTSSAI 499

Query: 601 HKLRDEELQPQLTEL 607
            ++RD EL PQL EL
Sbjct: 601 VRVRDNELAPQLVEL 499

BLAST of Lcy12g013940 vs. ExPASy TrEMBL
Match: A0A6J1IPD0 (nitrate regulatory gene2 protein-like OS=Cucurbita maxima OX=3661 GN=LOC111477012 PE=4 SV=1)

HSP 1 Score: 1017.3 bits (2629), Expect = 2.8e-293
Identity = 538/627 (85.81%), Postives = 569/627 (90.75%), Query Frame = 0

Query: 1   MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSLTDIGEAIRKFVDEEI 60
           MGCGGSKVDELQLV+LCRERKELIKAASRHRYALAAAHVTYFQSL DIGEAI KFVDEEI
Sbjct: 1   MGCGGSKVDELQLVSLCRERKELIKAASRHRYALAAAHVTYFQSLKDIGEAIHKFVDEEI 60

Query: 61  VISGADSSSTHGSPVLTLPSDEGKGKRKKPKSGEKHINSSSSSSILHSVSISHEPSPHED 120
           VISGA+SSS+HGSPVLTLPSDEGKGKRKKP SGE HI+SSSSS ILHS+SIS   SPHED
Sbjct: 61  VISGAESSSSHGSPVLTLPSDEGKGKRKKPTSGENHISSSSSSPILHSLSISQ--SPHED 120

Query: 121 EIDGSHLHLSSGSDSESEHHSSGHIQIEDSPVQDEGYSHPPYAYPPRDWSPP-------- 180
           EIDG HLHLSSGS+SESEH+SSGHIQIEDSP+ DEGYSHPPYAYPPRDWS P        
Sbjct: 121 EIDG-HLHLSSGSESESEHNSSGHIQIEDSPLHDEGYSHPPYAYPPRDWSSPTNSHPPYA 180

Query: 181 ---------TNTYAYYMQRSTTPAKTVMYDGPETHTASDGQWPDPSYSYPQYPQYENGGF 240
                    TNTYAYYMQRSTTPA TVMY+GPETHTASDGQWPDPS++YP Y QY NGGF
Sbjct: 181 YLPRDWGSSTNTYAYYMQRSTTPATTVMYNGPETHTASDGQWPDPSHNYPPYQQYGNGGF 240

Query: 241 YGFSMGSQPDYNLYNQQPRRPATPPPPPSPPKVSAWDFMNVFDGYDNVYQDYNAGNRYGY 300
           YGF MGS PDY+L+NQQPRRPATPPPPPSPPKVSAWDFMNVFD YDN YQ YN+ N+YGY
Sbjct: 241 YGFPMGSPPDYSLHNQQPRRPATPPPPPSPPKVSAWDFMNVFDDYDNGYQVYNSANKYGY 300

Query: 301 GSIQSSPDSNEVREREGIPELEDETEPEALKEIKERKKLNVETMNTNMNSGEGTSKFVTP 360
           GSIQSSPDSNEVREREGIPELEDETEPEALKEIKERKKLNVE +N NMNSGEGTSKFV  
Sbjct: 301 GSIQSSPDSNEVREREGIPELEDETEPEALKEIKERKKLNVEAINKNMNSGEGTSKFVPQ 360

Query: 361 HSSEDSTKSVPLPNSGSSPISKEKGIDNSPDTNVSKKSEQEEPVEKKELSFEIEETSTLD 420
            S+EDS+KSVPLPNSGSS +SKEKGI+NSPDT V KKSEQEE V KKE+SFEIEETSTLD
Sbjct: 361 QSNEDSSKSVPLPNSGSSTVSKEKGINNSPDTIVLKKSEQEESVGKKEVSFEIEETSTLD 420

Query: 421 IESSKKSNLATFTAYGTRDLQEVVNEIKDEFEAASNHGKEVAMLLEVGRLPYRSKIAVLK 480
           +ESSKKSNL  FTA+GTRDLQEVV+EIK EFEAAS++GKEVAMLLEVGRLPYRSKI VLK
Sbjct: 421 VESSKKSNLTAFTAFGTRDLQEVVSEIKVEFEAASSYGKEVAMLLEVGRLPYRSKITVLK 480

Query: 481 VILSRIQYLVAPSSASSQPPLIWLDPKTVKRAKAYSGSGSPGNELDLKSGSLSSTLEKLY 540
           +ILSRIQYLVAPSSASS+PPLIWLDPKTVK AKAYSGS SPGNELDLKSGSLSSTLEKLY
Sbjct: 481 IILSRIQYLVAPSSASSRPPLIWLDPKTVKMAKAYSGSSSPGNELDLKSGSLSSTLEKLY 540

Query: 541 VWEKKLYKEVKDEERLRVIYEKLCKKLKRLDDHGADSTKIDATHASIRKLSTKIDICIKA 600
            WEKKLYKEVKDEERLRVIYEKLCKKLKRLDDHGADSTKIDA HASIR+LSTKID+CIKA
Sbjct: 541 AWEKKLYKEVKDEERLRVIYEKLCKKLKRLDDHGADSTKIDAVHASIRRLSTKIDVCIKA 600

Query: 601 VDAISSRIHKLRDEELQPQLTELIHGY 611
            DAISS IHKLRDEELQPQLTELIHG+
Sbjct: 601 ADAISSSIHKLRDEELQPQLTELIHGW 624

BLAST of Lcy12g013940 vs. ExPASy TrEMBL
Match: A0A6J1F875 (nitrate regulatory gene2 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111443211 PE=4 SV=1)

HSP 1 Score: 1016.5 bits (2627), Expect = 4.8e-293
Identity = 539/627 (85.96%), Postives = 569/627 (90.75%), Query Frame = 0

Query: 1   MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSLTDIGEAIRKFVDEEI 60
           MGCGGSKVDELQLV+LCRERKELIKAASRHRYALAAAHVTYFQSL DIGEAI KFVDEEI
Sbjct: 1   MGCGGSKVDELQLVSLCRERKELIKAASRHRYALAAAHVTYFQSLKDIGEAIHKFVDEEI 60

Query: 61  VISGADSSSTHGSPVLTLPSDEGKGKRKKPKSGEKHINSSSSSSILHSVSISHEPSPHED 120
           VISGA+SSS+HGSPVLTLPSDEGKGKRKKP SGE HI+SSSSS ILHS+SIS   SPHED
Sbjct: 61  VISGAESSSSHGSPVLTLPSDEGKGKRKKPTSGENHISSSSSSPILHSLSISQ--SPHED 120

Query: 121 EIDGSHLHLSSGSDSESEHHSSGHIQIEDSPVQDEGYSHPPYAYPPRDWSPPT------- 180
           EIDG HLHLSSGS+SESEH+SSGHIQIEDSP+ DEGYSHPPYAYPPRDWS PT       
Sbjct: 121 EIDG-HLHLSSGSESESEHNSSGHIQIEDSPLHDEGYSHPPYAYPPRDWSSPTNSHPPYA 180

Query: 181 ----------NTYAYYMQRSTTPAKTVMYDGPETHTASDGQWPDPSYSYPQYPQYENGGF 240
                     NTYAYYMQRSTTPA TVMY+GPETHTASDGQWPDPS++YP Y QY NGGF
Sbjct: 181 YLPRDWSSSMNTYAYYMQRSTTPATTVMYNGPETHTASDGQWPDPSHNYPPYQQYGNGGF 240

Query: 241 YGFSMGSQPDYNLYNQQPRRPATPPPPPSPPKVSAWDFMNVFDGYDNVYQDYNAGNRYGY 300
           YGF MGS PDY+L+NQQPRRPATPPPPPSPP VSAWDFMNVFD YDN YQ YN+ N+YGY
Sbjct: 241 YGFPMGSPPDYSLHNQQPRRPATPPPPPSPPTVSAWDFMNVFDDYDNGYQVYNSANKYGY 300

Query: 301 GSIQSSPDSNEVREREGIPELEDETEPEALKEIKERKKLNVETMNTNMNSGEGTSKFVTP 360
           GSIQSSPDSNEVREREGIPELEDETEPEALKEIKERKKLNVE +  NMNSGEGTSKFV  
Sbjct: 301 GSIQSSPDSNEVREREGIPELEDETEPEALKEIKERKKLNVEAIKKNMNSGEGTSKFVPQ 360

Query: 361 HSSEDSTKSVPLPNSGSSPISKEKGIDNSPDTNVSKKSEQEEPVEKKELSFEIEETSTLD 420
            SSEDS+KSVPLPNSGSS +SKEKGI+NSPDT VSKKSEQEE V KKE+SFEIEETSTLD
Sbjct: 361 QSSEDSSKSVPLPNSGSSTVSKEKGINNSPDTIVSKKSEQEESVGKKEVSFEIEETSTLD 420

Query: 421 IESSKKSNLATFTAYGTRDLQEVVNEIKDEFEAASNHGKEVAMLLEVGRLPYRSKIAVLK 480
           +ESSKKSNL  FTA+GTRDLQEVV+EIK EFEAAS++GKEVAMLLEVGRLPYRSKIAVLK
Sbjct: 421 VESSKKSNLTAFTAFGTRDLQEVVSEIKVEFEAASSYGKEVAMLLEVGRLPYRSKIAVLK 480

Query: 481 VILSRIQYLVAPSSASSQPPLIWLDPKTVKRAKAYSGSGSPGNELDLKSGSLSSTLEKLY 540
           VILSRIQYLVAPSSASS+PPLIWLDPKTVK AKAYSGS SPGNELDLKSGSLSSTLEKLY
Sbjct: 481 VILSRIQYLVAPSSASSRPPLIWLDPKTVKMAKAYSGSSSPGNELDLKSGSLSSTLEKLY 540

Query: 541 VWEKKLYKEVKDEERLRVIYEKLCKKLKRLDDHGADSTKIDATHASIRKLSTKIDICIKA 600
            WEKKLYKEVKDEERLRVIYEKLCKKLKRLDDHGADS+KIDA HASIR+LSTKID+CIKA
Sbjct: 541 AWEKKLYKEVKDEERLRVIYEKLCKKLKRLDDHGADSSKIDAVHASIRRLSTKIDVCIKA 600

Query: 601 VDAISSRIHKLRDEELQPQLTELIHGY 611
            DAISS IHKLRDEELQPQLTELIHG+
Sbjct: 601 ADAISSSIHKLRDEELQPQLTELIHGW 624

BLAST of Lcy12g013940 vs. ExPASy TrEMBL
Match: A0A0A0L369 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G361900 PE=4 SV=1)

HSP 1 Score: 1006.5 bits (2601), Expect = 4.9e-290
Identity = 541/686 (78.86%), Postives = 574/686 (83.67%), Query Frame = 0

Query: 1   MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSLTDIGEAIRKFVDEEI 60
           MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSL DIGEAIRKFVDEEI
Sbjct: 1   MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSLKDIGEAIRKFVDEEI 60

Query: 61  VISGADSSSTHGSPVLTLPSDEGKGKRKKPKSGEKHINSSSSSSILHSVSISHEPSPHED 120
           VISGA+SSS+HGSPVLTLPSDEGKGKRKKPKSGEKHINSSSSSS+LHSVSISHE SPHED
Sbjct: 61  VISGAESSSSHGSPVLTLPSDEGKGKRKKPKSGEKHINSSSSSSVLHSVSISHEHSPHED 120

Query: 121 EIDGSHLHLSSGSDSESEHHSSGHIQIEDSPVQDEGYSHPPY------------------ 180
           EIDGSHLHLSSGS+SE EH+SSGHI IEDSPV DEGYSHPPY                  
Sbjct: 121 EIDGSHLHLSSGSESEYEHNSSGHIHIEDSPVHDEGYSHPPYAYPPKDIPAQDEGYSHLP 180

Query: 181 ----------------------------------------------------------AY 240
                                                                     AY
Sbjct: 181 YAYPPRDVPIQDEGYSHPPYAYPPRDIPVRDEGYSYPPFAFPPRDIPLQAEGYSHPPHAY 240

Query: 241 PPRDWSPPTNTYAYYMQRSTTPAKTVMYDGPETHTASDGQWPDPSYSYPQYPQYENGGFY 300
           PPRDWS  TNTYAY+MQ+STTPA TVMY+GPETHTASDGQWP PSYSYP Y QY NGGFY
Sbjct: 241 PPRDWS-STNTYAYFMQKSTTPATTVMYNGPETHTASDGQWPGPSYSYPPYAQYGNGGFY 300

Query: 301 GFSMGSQPDYNLYNQQPRRPATPPPPPSPPKVSAWDFMNVFDGYDNVYQDYNAGNRYGYG 360
           GFSMGS PDYN++NQQP+RPATPPPPPSPPKVSAWDFMNVFDGYDN YQ+YN+ NRYGYG
Sbjct: 301 GFSMGSPPDYNMHNQQPKRPATPPPPPSPPKVSAWDFMNVFDGYDNGYQEYNSANRYGYG 360

Query: 361 SIQSSPDSNEVREREGIPELEDETEPEALKEIKERKKLNVETMNTNMNSGEGTSKFVTPH 420
           SIQSSPDSNEVREREGIP+LEDETEPEALKEIKERKKL VE +N N+NSGEGTSKFV P 
Sbjct: 361 SIQSSPDSNEVREREGIPQLEDETEPEALKEIKERKKLKVEGINKNLNSGEGTSKFVPPE 420

Query: 421 SSEDSTKSVPLPNSGSSPISKEKGIDNSPDTNVSKKSEQEEPVEKKELSFEIEETSTLDI 480
           S ED +KSVPLPNS +S + KEKGI+NSPDT VSKKSE EEP+EKKE+SFEIEETSTLDI
Sbjct: 421 SGEDISKSVPLPNSVTSTVLKEKGINNSPDTIVSKKSEHEEPMEKKEVSFEIEETSTLDI 480

Query: 481 ESSKKSNLATFTAYGTRDLQEVVNEIKDEFEAASNHGKEVAMLLEVGRLPYRSKIAVLKV 540
           ESSKKSNLATF A+GTRDLQEVV+EIK+EFEAAS+ GKEVAMLLEVGRLPYRSKI VLKV
Sbjct: 481 ESSKKSNLATFAAFGTRDLQEVVSEIKNEFEAASSCGKEVAMLLEVGRLPYRSKITVLKV 540

Query: 541 ILSRIQYLVAPSSASSQPPLIWLDPKTVKRAKAYSGSGSPGNELDLKSGSLSSTLEKLYV 600
           ILSRIQYLVAPSS SSQPPLI LDPKTVK AKAY+GS SPGN+ D+KSGSLSSTLEKLYV
Sbjct: 541 ILSRIQYLVAPSSVSSQPPLIRLDPKTVKMAKAYAGSSSPGNDFDMKSGSLSSTLEKLYV 600

Query: 601 WEKKLYKEVKDEERLRVIYEKLCKKLKRLDDHGADSTKIDATHASIRKLSTKIDICIKAV 611
           WEKKLYKEVKDEERLRVIYEKLCKKLKRLD+HGADSTKIDATHASIRKLSTKID+CIKA 
Sbjct: 601 WEKKLYKEVKDEERLRVIYEKLCKKLKRLDEHGADSTKIDATHASIRKLSTKIDVCIKAA 660

BLAST of Lcy12g013940 vs. ExPASy TrEMBL
Match: A0A1S3B9R2 (uncharacterized protein LOC103487594 OS=Cucumis melo OX=3656 GN=LOC103487594 PE=4 SV=1)

HSP 1 Score: 997.7 bits (2578), Expect = 2.3e-287
Identity = 538/687 (78.31%), Postives = 570/687 (82.97%), Query Frame = 0

Query: 1   MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSLTDIGEAIRKFVDEEI 60
           MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSL DIGEAIRKFVDEEI
Sbjct: 1   MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSLKDIGEAIRKFVDEEI 60

Query: 61  VISGADSSSTHGSPVLTLPSDEGKGKRKKPKSGEKHINSSSSSSILHSVSISHEPSPHED 120
           VISGA+SSS+HGSPVLTLPSDEGKGKRKKPKSGEKHINSSSSSS+LHSVSISHE SPHED
Sbjct: 61  VISGAESSSSHGSPVLTLPSDEGKGKRKKPKSGEKHINSSSSSSVLHSVSISHEHSPHED 120

Query: 121 EIDGSHLHLSSGSDSESEHHSSGHIQIEDSPVQDEGYSHPPYAY---------------- 180
           EIDGSHLHLSSGS+SE E +SSGHI IEDSPV DEGYSHPPYAY                
Sbjct: 121 EIDGSHLHLSSGSESEYEQNSSGHIHIEDSPVHDEGYSHPPYAYPPKDIPAQDEGYSHLP 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 YAYPPRDVSIQDEGYSHPPYAYPPRDIPVRDEGYRYAPYAYPPRDIPLEVDGSSHPPPFA 240

Query: 241 -PPRDWSPPTNTYAYYMQRSTTPAKTVMYDGPETHTASDGQWPDPSYSYPQYPQYENGGF 300
            PPRDWS  TNTYAY+MQ+STTPA TVMY+GPETHTASDGQWP PSYSYP Y QY NGGF
Sbjct: 241 SPPRDWS-STNTYAYFMQKSTTPATTVMYNGPETHTASDGQWPGPSYSYPPYAQYGNGGF 300

Query: 301 YGFSMGSQPDYNLYNQQPRRPATPPPPPSPPKVSAWDFMNVFDGYDNVYQDYNAGNRYGY 360
           YGFSMGS PDYN++NQQP+RPATPPPPPSPPKVSAWDFMNVFDGYDN YQ+YN+  RYGY
Sbjct: 301 YGFSMGSPPDYNMHNQQPKRPATPPPPPSPPKVSAWDFMNVFDGYDNGYQEYNSAIRYGY 360

Query: 361 GSIQSSPDSNEVREREGIPELEDETEPEALKEIKERKKLNVETMNTNMNSGEGTSKFVTP 420
           GSIQSSPDSNEVREREGIP+LEDETEPEALKEIKERKKL VE  N NMNSGEGTSKFV P
Sbjct: 361 GSIQSSPDSNEVREREGIPQLEDETEPEALKEIKERKKLKVEGTNKNMNSGEGTSKFVPP 420

Query: 421 HSSEDSTKSVPLPNSGSSPISKEKGIDNSPDTNVSKKSEQEEPVEKKELSFEIEETSTLD 480
            S E+ +KSVPLPNSG+S + KEKGI+NSPDT VSK SE EEP+EKKE+SFEIEETSTLD
Sbjct: 421 ESGEEISKSVPLPNSGTSTVLKEKGINNSPDTIVSKTSEHEEPMEKKEVSFEIEETSTLD 480

Query: 481 IESSKKSNLATFTAYGTRDLQEVVNEIKDEFEAASNHGKEVAMLLEVGRLPYRSKIAVLK 540
           +ESSKK+NLATF A+GTRDLQEVV+EIK+EFEAAS+ GKEVAMLLEVGRLPYRSKI VLK
Sbjct: 481 VESSKKTNLATFAAFGTRDLQEVVSEIKNEFEAASSCGKEVAMLLEVGRLPYRSKITVLK 540

Query: 541 VILSRIQYLVAPSSASSQPPLIWLDPKTVKRAKAYSGSGSPGNELDLKSGSLSSTLEKLY 600
           VILSRIQYLVAPSSASSQPPLI LDPKTVK AK Y+GS SPGNE DLKSGSLSSTLEKLY
Sbjct: 541 VILSRIQYLVAPSSASSQPPLIRLDPKTVKMAKEYAGSSSPGNEFDLKSGSLSSTLEKLY 600

Query: 601 VWEKKLYKEVKDEERLRVIYEKLCKKLKRLDDHGADSTKIDATHASIRKLSTKIDICIKA 611
           VWEKKLYKEVKDEERLR+IYEKLCKKLKRLDDHGADSTKIDATHASIRKLSTKID+CIKA
Sbjct: 601 VWEKKLYKEVKDEERLRIIYEKLCKKLKRLDDHGADSTKIDATHASIRKLSTKIDVCIKA 660

BLAST of Lcy12g013940 vs. ExPASy TrEMBL
Match: A0A6J1BUL6 (uncharacterized protein LOC111004881 OS=Momordica charantia OX=3673 GN=LOC111004881 PE=4 SV=1)

HSP 1 Score: 989.6 bits (2557), Expect = 6.2e-285
Identity = 519/612 (84.80%), Postives = 555/612 (90.69%), Query Frame = 0

Query: 1   MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSLTDIGEAIRKFVDEEI 60
           MGCGGSKVDELQLV LCRERKELIKAASRHRYALAAAHVTYFQSL DIGEAIRKFVDEEI
Sbjct: 1   MGCGGSKVDELQLVNLCRERKELIKAASRHRYALAAAHVTYFQSLKDIGEAIRKFVDEEI 60

Query: 61  VISGADSSSTHGSPVLTLPSDEGKGKRKKPKSGEKHINSSSSSSILHSVSISHEPSPHED 120
           VISG DSSS+HGSPVLTLPSDEGKGKRKKPKSGEKH NSSSS+SI HSVSISHE SP ED
Sbjct: 61  VISGVDSSSSHGSPVLTLPSDEGKGKRKKPKSGEKHGNSSSSTSISHSVSISHEHSPQED 120

Query: 121 EIDGSHLHLSSGSDSESEHHSSGHIQIEDSPVQDEGY--SHPPYAYPPRDWSPPTNTYAY 180
           EI+GSHLH+SSGS++ES H+SSGHI IEDSP +D GY  SHPPYAYPP DW+PP NTYAY
Sbjct: 121 EIEGSHLHISSGSETESLHNSSGHIHIEDSPEEDGGYSHSHPPYAYPPSDWNPPVNTYAY 180

Query: 181 YMQRSTTPAKTVMYDGPETHTASDGQWPDPSYSYPQYPQYENGGFYGFSMGSQPDYNLYN 240
           YMQRSTTPA TV YDGPETHTASDG WPDPSYSY  YPQY NGGF+G+SMGS P+YN YN
Sbjct: 181 YMQRSTTPATTVTYDGPETHTASDGPWPDPSYSYSAYPQYGNGGFFGYSMGSPPNYNPYN 240

Query: 241 QQPRRPATPPPPPSPPKVSAWDFMNVFDGYDNVYQDYNAGNRYGYGSIQSSPDSNEVRER 300
           QQP RPA PPPPPSPPKVS WDF+NVF+GYD  Y DYN+ NRYGYGSIQSSPDSNEVRER
Sbjct: 241 QQPSRPAPPPPPPSPPKVSTWDFINVFEGYDYGYPDYNSQNRYGYGSIQSSPDSNEVRER 300

Query: 301 EGIPELEDETEPEALKEIKERKKLNVETMNTNMNSGEGTSKFVTPHSSEDSTKSVPLPNS 360
           EGIPELEDETEPE      ERKKLNVET++ NMNSGEGTS FV P SSEDS+ SVPL NS
Sbjct: 301 EGIPELEDETEPET----HERKKLNVETVDKNMNSGEGTSNFVPPRSSEDSSNSVPLQNS 360

Query: 361 GSSPISKEKGIDNSPDTNVSKKSEQEEPVEKKELSFEIEETSTLDIESSKKSNLATFTAY 420
           GSS +SKE+GI+NSPDT VSKK E+EEPVEKKE+SFEIEETSTLDIESSKKSNLATF A+
Sbjct: 361 GSSVLSKEQGINNSPDTIVSKKLEEEEPVEKKEVSFEIEETSTLDIESSKKSNLATFIAH 420

Query: 421 GTRDLQEVVNEIKDEFEAASNHGKEVAMLLEVGRLPYRSKIAVLKVILSRIQYLVAPSSA 480
           GTRD+QEVVNEIKDEFEAAS++GKEVAMLLEVG LPYRSKI VLKV+LSRI YLVAPS+A
Sbjct: 421 GTRDIQEVVNEIKDEFEAASSYGKEVAMLLEVGSLPYRSKITVLKVVLSRILYLVAPSAA 480

Query: 481 SSQPPLIWLDPKTVKRAKAYSGSGSPGNELDLKSGSLSSTLEKLYVWEKKLYKEVKDEER 540
           SSQPPL+WLD KTVK AKAYSGSGS GNE +LKSG+LS+TLEKLY WEKKLYKEVKDEE+
Sbjct: 481 SSQPPLVWLDSKTVKMAKAYSGSGSSGNEFNLKSGNLSTTLEKLYAWEKKLYKEVKDEEK 540

Query: 541 LRVIYEKLCKKLKRLDDHGADSTKIDATHASIRKLSTKIDICIKAVDAISSRIHKLRDEE 600
           LRVIYEK+CKKLKRLDD GADS KIDATHASIRKLSTKIDICIKA DAISSRIHKLRDEE
Sbjct: 541 LRVIYEKMCKKLKRLDDRGADSNKIDATHASIRKLSTKIDICIKAADAISSRIHKLRDEE 600

Query: 601 LQPQLTELIHGY 611
           LQPQLTELIHG+
Sbjct: 601 LQPQLTELIHGW 608

BLAST of Lcy12g013940 vs. NCBI nr
Match: XP_022976699.1 (nitrate regulatory gene2 protein-like [Cucurbita maxima] >XP_022976700.1 nitrate regulatory gene2 protein-like [Cucurbita maxima])

HSP 1 Score: 1017.3 bits (2629), Expect = 5.8e-293
Identity = 538/627 (85.81%), Postives = 569/627 (90.75%), Query Frame = 0

Query: 1   MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSLTDIGEAIRKFVDEEI 60
           MGCGGSKVDELQLV+LCRERKELIKAASRHRYALAAAHVTYFQSL DIGEAI KFVDEEI
Sbjct: 1   MGCGGSKVDELQLVSLCRERKELIKAASRHRYALAAAHVTYFQSLKDIGEAIHKFVDEEI 60

Query: 61  VISGADSSSTHGSPVLTLPSDEGKGKRKKPKSGEKHINSSSSSSILHSVSISHEPSPHED 120
           VISGA+SSS+HGSPVLTLPSDEGKGKRKKP SGE HI+SSSSS ILHS+SIS   SPHED
Sbjct: 61  VISGAESSSSHGSPVLTLPSDEGKGKRKKPTSGENHISSSSSSPILHSLSISQ--SPHED 120

Query: 121 EIDGSHLHLSSGSDSESEHHSSGHIQIEDSPVQDEGYSHPPYAYPPRDWSPP-------- 180
           EIDG HLHLSSGS+SESEH+SSGHIQIEDSP+ DEGYSHPPYAYPPRDWS P        
Sbjct: 121 EIDG-HLHLSSGSESESEHNSSGHIQIEDSPLHDEGYSHPPYAYPPRDWSSPTNSHPPYA 180

Query: 181 ---------TNTYAYYMQRSTTPAKTVMYDGPETHTASDGQWPDPSYSYPQYPQYENGGF 240
                    TNTYAYYMQRSTTPA TVMY+GPETHTASDGQWPDPS++YP Y QY NGGF
Sbjct: 181 YLPRDWGSSTNTYAYYMQRSTTPATTVMYNGPETHTASDGQWPDPSHNYPPYQQYGNGGF 240

Query: 241 YGFSMGSQPDYNLYNQQPRRPATPPPPPSPPKVSAWDFMNVFDGYDNVYQDYNAGNRYGY 300
           YGF MGS PDY+L+NQQPRRPATPPPPPSPPKVSAWDFMNVFD YDN YQ YN+ N+YGY
Sbjct: 241 YGFPMGSPPDYSLHNQQPRRPATPPPPPSPPKVSAWDFMNVFDDYDNGYQVYNSANKYGY 300

Query: 301 GSIQSSPDSNEVREREGIPELEDETEPEALKEIKERKKLNVETMNTNMNSGEGTSKFVTP 360
           GSIQSSPDSNEVREREGIPELEDETEPEALKEIKERKKLNVE +N NMNSGEGTSKFV  
Sbjct: 301 GSIQSSPDSNEVREREGIPELEDETEPEALKEIKERKKLNVEAINKNMNSGEGTSKFVPQ 360

Query: 361 HSSEDSTKSVPLPNSGSSPISKEKGIDNSPDTNVSKKSEQEEPVEKKELSFEIEETSTLD 420
            S+EDS+KSVPLPNSGSS +SKEKGI+NSPDT V KKSEQEE V KKE+SFEIEETSTLD
Sbjct: 361 QSNEDSSKSVPLPNSGSSTVSKEKGINNSPDTIVLKKSEQEESVGKKEVSFEIEETSTLD 420

Query: 421 IESSKKSNLATFTAYGTRDLQEVVNEIKDEFEAASNHGKEVAMLLEVGRLPYRSKIAVLK 480
           +ESSKKSNL  FTA+GTRDLQEVV+EIK EFEAAS++GKEVAMLLEVGRLPYRSKI VLK
Sbjct: 421 VESSKKSNLTAFTAFGTRDLQEVVSEIKVEFEAASSYGKEVAMLLEVGRLPYRSKITVLK 480

Query: 481 VILSRIQYLVAPSSASSQPPLIWLDPKTVKRAKAYSGSGSPGNELDLKSGSLSSTLEKLY 540
           +ILSRIQYLVAPSSASS+PPLIWLDPKTVK AKAYSGS SPGNELDLKSGSLSSTLEKLY
Sbjct: 481 IILSRIQYLVAPSSASSRPPLIWLDPKTVKMAKAYSGSSSPGNELDLKSGSLSSTLEKLY 540

Query: 541 VWEKKLYKEVKDEERLRVIYEKLCKKLKRLDDHGADSTKIDATHASIRKLSTKIDICIKA 600
            WEKKLYKEVKDEERLRVIYEKLCKKLKRLDDHGADSTKIDA HASIR+LSTKID+CIKA
Sbjct: 541 AWEKKLYKEVKDEERLRVIYEKLCKKLKRLDDHGADSTKIDAVHASIRRLSTKIDVCIKA 600

Query: 601 VDAISSRIHKLRDEELQPQLTELIHGY 611
            DAISS IHKLRDEELQPQLTELIHG+
Sbjct: 601 ADAISSSIHKLRDEELQPQLTELIHGW 624

BLAST of Lcy12g013940 vs. NCBI nr
Match: XP_022936696.1 (nitrate regulatory gene2 protein-like [Cucurbita moschata] >XP_022936697.1 nitrate regulatory gene2 protein-like [Cucurbita moschata])

HSP 1 Score: 1016.5 bits (2627), Expect = 9.8e-293
Identity = 539/627 (85.96%), Postives = 569/627 (90.75%), Query Frame = 0

Query: 1   MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSLTDIGEAIRKFVDEEI 60
           MGCGGSKVDELQLV+LCRERKELIKAASRHRYALAAAHVTYFQSL DIGEAI KFVDEEI
Sbjct: 1   MGCGGSKVDELQLVSLCRERKELIKAASRHRYALAAAHVTYFQSLKDIGEAIHKFVDEEI 60

Query: 61  VISGADSSSTHGSPVLTLPSDEGKGKRKKPKSGEKHINSSSSSSILHSVSISHEPSPHED 120
           VISGA+SSS+HGSPVLTLPSDEGKGKRKKP SGE HI+SSSSS ILHS+SIS   SPHED
Sbjct: 61  VISGAESSSSHGSPVLTLPSDEGKGKRKKPTSGENHISSSSSSPILHSLSISQ--SPHED 120

Query: 121 EIDGSHLHLSSGSDSESEHHSSGHIQIEDSPVQDEGYSHPPYAYPPRDWSPPT------- 180
           EIDG HLHLSSGS+SESEH+SSGHIQIEDSP+ DEGYSHPPYAYPPRDWS PT       
Sbjct: 121 EIDG-HLHLSSGSESESEHNSSGHIQIEDSPLHDEGYSHPPYAYPPRDWSSPTNSHPPYA 180

Query: 181 ----------NTYAYYMQRSTTPAKTVMYDGPETHTASDGQWPDPSYSYPQYPQYENGGF 240
                     NTYAYYMQRSTTPA TVMY+GPETHTASDGQWPDPS++YP Y QY NGGF
Sbjct: 181 YLPRDWSSSMNTYAYYMQRSTTPATTVMYNGPETHTASDGQWPDPSHNYPPYQQYGNGGF 240

Query: 241 YGFSMGSQPDYNLYNQQPRRPATPPPPPSPPKVSAWDFMNVFDGYDNVYQDYNAGNRYGY 300
           YGF MGS PDY+L+NQQPRRPATPPPPPSPP VSAWDFMNVFD YDN YQ YN+ N+YGY
Sbjct: 241 YGFPMGSPPDYSLHNQQPRRPATPPPPPSPPTVSAWDFMNVFDDYDNGYQVYNSANKYGY 300

Query: 301 GSIQSSPDSNEVREREGIPELEDETEPEALKEIKERKKLNVETMNTNMNSGEGTSKFVTP 360
           GSIQSSPDSNEVREREGIPELEDETEPEALKEIKERKKLNVE +  NMNSGEGTSKFV  
Sbjct: 301 GSIQSSPDSNEVREREGIPELEDETEPEALKEIKERKKLNVEAIKKNMNSGEGTSKFVPQ 360

Query: 361 HSSEDSTKSVPLPNSGSSPISKEKGIDNSPDTNVSKKSEQEEPVEKKELSFEIEETSTLD 420
            SSEDS+KSVPLPNSGSS +SKEKGI+NSPDT VSKKSEQEE V KKE+SFEIEETSTLD
Sbjct: 361 QSSEDSSKSVPLPNSGSSTVSKEKGINNSPDTIVSKKSEQEESVGKKEVSFEIEETSTLD 420

Query: 421 IESSKKSNLATFTAYGTRDLQEVVNEIKDEFEAASNHGKEVAMLLEVGRLPYRSKIAVLK 480
           +ESSKKSNL  FTA+GTRDLQEVV+EIK EFEAAS++GKEVAMLLEVGRLPYRSKIAVLK
Sbjct: 421 VESSKKSNLTAFTAFGTRDLQEVVSEIKVEFEAASSYGKEVAMLLEVGRLPYRSKIAVLK 480

Query: 481 VILSRIQYLVAPSSASSQPPLIWLDPKTVKRAKAYSGSGSPGNELDLKSGSLSSTLEKLY 540
           VILSRIQYLVAPSSASS+PPLIWLDPKTVK AKAYSGS SPGNELDLKSGSLSSTLEKLY
Sbjct: 481 VILSRIQYLVAPSSASSRPPLIWLDPKTVKMAKAYSGSSSPGNELDLKSGSLSSTLEKLY 540

Query: 541 VWEKKLYKEVKDEERLRVIYEKLCKKLKRLDDHGADSTKIDATHASIRKLSTKIDICIKA 600
            WEKKLYKEVKDEERLRVIYEKLCKKLKRLDDHGADS+KIDA HASIR+LSTKID+CIKA
Sbjct: 541 AWEKKLYKEVKDEERLRVIYEKLCKKLKRLDDHGADSSKIDAVHASIRRLSTKIDVCIKA 600

Query: 601 VDAISSRIHKLRDEELQPQLTELIHGY 611
            DAISS IHKLRDEELQPQLTELIHG+
Sbjct: 601 ADAISSSIHKLRDEELQPQLTELIHGW 624

BLAST of Lcy12g013940 vs. NCBI nr
Match: XP_023535799.1 (nitrate regulatory gene2 protein [Cucurbita pepo subsp. pepo] >XP_023535800.1 nitrate regulatory gene2 protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1013.8 bits (2620), Expect = 6.4e-292
Identity = 539/627 (85.96%), Postives = 569/627 (90.75%), Query Frame = 0

Query: 1   MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSLTDIGEAIRKFVDEEI 60
           MGCGGSKVDELQLV+LCRERKELIKAASRHRYALAAAHVTYFQSL DIGEAI KFVDEEI
Sbjct: 1   MGCGGSKVDELQLVSLCRERKELIKAASRHRYALAAAHVTYFQSLKDIGEAIHKFVDEEI 60

Query: 61  VISGADSSSTHGSPVLTLPSDEGKGKRKKPKSGEKHINSSSSSSILHSVSISHEPSPHED 120
           VISGA+SSS+HGSPVLTLPSDEGKGKRKKP SGE HI+SSSSS ILHS+SIS   SPHED
Sbjct: 61  VISGAESSSSHGSPVLTLPSDEGKGKRKKPTSGENHISSSSSSPILHSLSISQ--SPHED 120

Query: 121 EIDGSHLHLSSGSDSESEHHSSGHIQIEDSPVQDEGYSHPPYAYPPRDWSPP-------- 180
           EI+G HLHLSSGS+SESEH+SSGHIQIEDSP+ DEGYSHPPYAYPPRDWS P        
Sbjct: 121 EING-HLHLSSGSESESEHNSSGHIQIEDSPLHDEGYSHPPYAYPPRDWSSPMNSHPPFA 180

Query: 181 ---------TNTYAYYMQRSTTPAKTVMYDGPETHTASDGQWPDPSYSYPQYPQYENGGF 240
                    TNTYAYYMQRSTTPA TVMY+GPETHTASDGQWPDPS++YP Y QY NGGF
Sbjct: 181 YPPRDWSSSTNTYAYYMQRSTTPATTVMYNGPETHTASDGQWPDPSHNYPPYQQYGNGGF 240

Query: 241 YGFSMGSQPDYNLYNQQPRRPATPPPPPSPPKVSAWDFMNVFDGYDNVYQDYNAGNRYGY 300
           YGF MGS PDY+L+NQQPRR AT PPPPSPPKVSAWDFMNVFD YDN YQ YN+GN+YGY
Sbjct: 241 YGFPMGSPPDYSLHNQQPRRTATSPPPPSPPKVSAWDFMNVFDDYDNGYQVYNSGNKYGY 300

Query: 301 GSIQSSPDSNEVREREGIPELEDETEPEALKEIKERKKLNVETMNTNMNSGEGTSKFVTP 360
           GSIQSSPDSNEVREREGIPELEDETEPEALKEIKERKKLNVE +  NMNSGEGTSKFV  
Sbjct: 301 GSIQSSPDSNEVREREGIPELEDETEPEALKEIKERKKLNVEAIKKNMNSGEGTSKFVPQ 360

Query: 361 HSSEDSTKSVPLPNSGSSPISKEKGIDNSPDTNVSKKSEQEEPVEKKELSFEIEETSTLD 420
            SSEDS+KSVPLPNSGSS +SKEKGI+NSPDT VSKKSEQEE V KKE+SFEIEETSTLD
Sbjct: 361 QSSEDSSKSVPLPNSGSSTVSKEKGINNSPDTIVSKKSEQEESVGKKEVSFEIEETSTLD 420

Query: 421 IESSKKSNLATFTAYGTRDLQEVVNEIKDEFEAASNHGKEVAMLLEVGRLPYRSKIAVLK 480
           +ESSKKSNL  FTA+GTRDLQEVV+EIK EFEAAS++GKEVAMLLEVGRLPYRSKIAVLK
Sbjct: 421 VESSKKSNLTAFTAFGTRDLQEVVSEIKVEFEAASSYGKEVAMLLEVGRLPYRSKIAVLK 480

Query: 481 VILSRIQYLVAPSSASSQPPLIWLDPKTVKRAKAYSGSGSPGNELDLKSGSLSSTLEKLY 540
           VILSRIQYLVAPSSASS+PPLIWLDPKTVK AKAYSGSGSPGNELDLKSGSLSSTLEKLY
Sbjct: 481 VILSRIQYLVAPSSASSRPPLIWLDPKTVKMAKAYSGSGSPGNELDLKSGSLSSTLEKLY 540

Query: 541 VWEKKLYKEVKDEERLRVIYEKLCKKLKRLDDHGADSTKIDATHASIRKLSTKIDICIKA 600
            WEKKLYKEVKDEERLRVIYEKLCKKLKRLDD GADSTKIDA HASIR+LSTKID+CIKA
Sbjct: 541 AWEKKLYKEVKDEERLRVIYEKLCKKLKRLDDQGADSTKIDAVHASIRRLSTKIDVCIKA 600

Query: 601 VDAISSRIHKLRDEELQPQLTELIHGY 611
            DAISS IHKLRDEELQPQLTELIHG+
Sbjct: 601 ADAISSSIHKLRDEELQPQLTELIHGW 624

BLAST of Lcy12g013940 vs. NCBI nr
Match: XP_038899197.1 (nitrate regulatory gene2 protein [Benincasa hispida] >XP_038899198.1 nitrate regulatory gene2 protein [Benincasa hispida] >XP_038899199.1 nitrate regulatory gene2 protein [Benincasa hispida])

HSP 1 Score: 1010.7 bits (2612), Expect = 5.4e-291
Identity = 550/687 (80.06%), Postives = 576/687 (83.84%), Query Frame = 0

Query: 1   MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSLTDIGEAIRKFVDEEI 60
           MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSL DIGEAIRKFVDEEI
Sbjct: 1   MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSLKDIGEAIRKFVDEEI 60

Query: 61  VISGADSSSTHGSPVLTLPSDEGKGKRKKPKSGEKHINSSSSSSILHSVSISHEPSPHED 120
           VISGA+SSS+HGSPVLTLPSDEGKGKRKKPKSGEKHINSSSSSS+LHSVSISHEPSPHED
Sbjct: 61  VISGAESSSSHGSPVLTLPSDEGKGKRKKPKSGEKHINSSSSSSVLHSVSISHEPSPHED 120

Query: 121 EIDGSHLHLSSGSDSESEHHSSGHIQIEDS------------------------------ 180
           EIDGSHLHLSSGS+SESEH+SSGHI IEDS                              
Sbjct: 121 EIDGSHLHLSSGSESESEHNSSGHIHIEDSPVHDEVHSHPPYAYPPRDTPFQEEVYSHPT 180

Query: 181 ---------------------------PVQDEGYSH--------------------PPYA 240
                                      PVQDEGYSH                    PPYA
Sbjct: 181 YAYPPRNVPVQDEGHSHPSYAYPPRDIPVQDEGYSHPPFAYPPRDIPVQVEGYSHPPPYA 240

Query: 241 YPPRDWSPPTNTYAYYMQRSTTPAKTVMYDGPETHTASDGQWPDPSYSYPQYPQYENGGF 300
           YPPRDWS  TNTYAYYMQ+STTPA TVMYDGPETHTASDGQ P PSYSYP Y QY NGGF
Sbjct: 241 YPPRDWS-STNTYAYYMQKSTTPATTVMYDGPETHTASDGQLPGPSYSYPPYMQYGNGGF 300

Query: 301 YGFSMGSQPDYNLYNQQPRRPATPPPPPSPPKVSAWDFMNVFDGYDNVYQDYNAGNRYGY 360
           YGFSMGS PDY++ +QQPRRPATPPPPPSPPKVSAWDFMNVFDGYDN YQDYN+GNRYGY
Sbjct: 301 YGFSMGSPPDYSMPSQQPRRPATPPPPPSPPKVSAWDFMNVFDGYDNGYQDYNSGNRYGY 360

Query: 361 GSIQSSPDSNEVREREGIPELEDETEPEALKEIKERKKLNVETMNTNMNSGEGTSKFVTP 420
           GSIQSSPDSNEVREREGIPELEDETEPEAL+EIKERKKL VE MN N+N GEGTSKFV P
Sbjct: 361 GSIQSSPDSNEVREREGIPELEDETEPEALEEIKERKKLKVEAMNKNLNLGEGTSKFVPP 420

Query: 421 HSSEDSTKSVPLPNSGSSPISKEKGIDNSPDTNVSKKSEQEEPVEKKELSFEIEETSTLD 480
            SS DS+K VPLPNS SS +SK+KGI+NSPDT VSKKSEQ EPVEKKE+SFEIEETSTLD
Sbjct: 421 ESSGDSSKPVPLPNSDSSTVSKQKGINNSPDTLVSKKSEQ-EPVEKKEVSFEIEETSTLD 480

Query: 481 IESSKKSNLATFTAYGTRDLQEVVNEIKDEFEAASNHGKEVAMLLEVGRLPYRSKIAVLK 540
           IESSKKSNLATF A+GTRDLQEVV+EIKDEFEAAS +GKEVAMLLEVGRLPYRSKI VLK
Sbjct: 481 IESSKKSNLATFAAFGTRDLQEVVSEIKDEFEAASGYGKEVAMLLEVGRLPYRSKITVLK 540

Query: 541 VILSRIQYLVAPSSASSQPPLIWLDPKTVKRAKAYSGSGSPGNELDLKSGSLSSTLEKLY 600
           VILSRIQYLVAPSSASSQPPLIWLDPKTVK AKAY+G  SPGNE DLKSGSLSSTLEKLY
Sbjct: 541 VILSRIQYLVAPSSASSQPPLIWLDPKTVKMAKAYTGLSSPGNEFDLKSGSLSSTLEKLY 600

Query: 601 VWEKKLYKEVKDEERLRVIYEKLCKKLKRLDDHGADSTKIDATHASIRKLSTKIDICIKA 611
           VWEKKLYKEVKDEERLRVIYEKLCKKLKRLD+HGADS+KIDATHASIRKLSTKID+CIKA
Sbjct: 601 VWEKKLYKEVKDEERLRVIYEKLCKKLKRLDEHGADSSKIDATHASIRKLSTKIDVCIKA 660

BLAST of Lcy12g013940 vs. NCBI nr
Match: XP_011653788.1 (nitrate regulatory gene2 protein [Cucumis sativus] >XP_011653789.1 nitrate regulatory gene2 protein [Cucumis sativus] >XP_011653790.1 nitrate regulatory gene2 protein [Cucumis sativus] >XP_011653791.1 nitrate regulatory gene2 protein [Cucumis sativus] >KGN54556.1 hypothetical protein Csa_012610 [Cucumis sativus])

HSP 1 Score: 1006.5 bits (2601), Expect = 1.0e-289
Identity = 541/686 (78.86%), Postives = 574/686 (83.67%), Query Frame = 0

Query: 1   MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSLTDIGEAIRKFVDEEI 60
           MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSL DIGEAIRKFVDEEI
Sbjct: 1   MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSLKDIGEAIRKFVDEEI 60

Query: 61  VISGADSSSTHGSPVLTLPSDEGKGKRKKPKSGEKHINSSSSSSILHSVSISHEPSPHED 120
           VISGA+SSS+HGSPVLTLPSDEGKGKRKKPKSGEKHINSSSSSS+LHSVSISHE SPHED
Sbjct: 61  VISGAESSSSHGSPVLTLPSDEGKGKRKKPKSGEKHINSSSSSSVLHSVSISHEHSPHED 120

Query: 121 EIDGSHLHLSSGSDSESEHHSSGHIQIEDSPVQDEGYSHPPY------------------ 180
           EIDGSHLHLSSGS+SE EH+SSGHI IEDSPV DEGYSHPPY                  
Sbjct: 121 EIDGSHLHLSSGSESEYEHNSSGHIHIEDSPVHDEGYSHPPYAYPPKDIPAQDEGYSHLP 180

Query: 181 ----------------------------------------------------------AY 240
                                                                     AY
Sbjct: 181 YAYPPRDVPIQDEGYSHPPYAYPPRDIPVRDEGYSYPPFAFPPRDIPLQAEGYSHPPHAY 240

Query: 241 PPRDWSPPTNTYAYYMQRSTTPAKTVMYDGPETHTASDGQWPDPSYSYPQYPQYENGGFY 300
           PPRDWS  TNTYAY+MQ+STTPA TVMY+GPETHTASDGQWP PSYSYP Y QY NGGFY
Sbjct: 241 PPRDWS-STNTYAYFMQKSTTPATTVMYNGPETHTASDGQWPGPSYSYPPYAQYGNGGFY 300

Query: 301 GFSMGSQPDYNLYNQQPRRPATPPPPPSPPKVSAWDFMNVFDGYDNVYQDYNAGNRYGYG 360
           GFSMGS PDYN++NQQP+RPATPPPPPSPPKVSAWDFMNVFDGYDN YQ+YN+ NRYGYG
Sbjct: 301 GFSMGSPPDYNMHNQQPKRPATPPPPPSPPKVSAWDFMNVFDGYDNGYQEYNSANRYGYG 360

Query: 361 SIQSSPDSNEVREREGIPELEDETEPEALKEIKERKKLNVETMNTNMNSGEGTSKFVTPH 420
           SIQSSPDSNEVREREGIP+LEDETEPEALKEIKERKKL VE +N N+NSGEGTSKFV P 
Sbjct: 361 SIQSSPDSNEVREREGIPQLEDETEPEALKEIKERKKLKVEGINKNLNSGEGTSKFVPPE 420

Query: 421 SSEDSTKSVPLPNSGSSPISKEKGIDNSPDTNVSKKSEQEEPVEKKELSFEIEETSTLDI 480
           S ED +KSVPLPNS +S + KEKGI+NSPDT VSKKSE EEP+EKKE+SFEIEETSTLDI
Sbjct: 421 SGEDISKSVPLPNSVTSTVLKEKGINNSPDTIVSKKSEHEEPMEKKEVSFEIEETSTLDI 480

Query: 481 ESSKKSNLATFTAYGTRDLQEVVNEIKDEFEAASNHGKEVAMLLEVGRLPYRSKIAVLKV 540
           ESSKKSNLATF A+GTRDLQEVV+EIK+EFEAAS+ GKEVAMLLEVGRLPYRSKI VLKV
Sbjct: 481 ESSKKSNLATFAAFGTRDLQEVVSEIKNEFEAASSCGKEVAMLLEVGRLPYRSKITVLKV 540

Query: 541 ILSRIQYLVAPSSASSQPPLIWLDPKTVKRAKAYSGSGSPGNELDLKSGSLSSTLEKLYV 600
           ILSRIQYLVAPSS SSQPPLI LDPKTVK AKAY+GS SPGN+ D+KSGSLSSTLEKLYV
Sbjct: 541 ILSRIQYLVAPSSVSSQPPLIRLDPKTVKMAKAYAGSSSPGNDFDMKSGSLSSTLEKLYV 600

Query: 601 WEKKLYKEVKDEERLRVIYEKLCKKLKRLDDHGADSTKIDATHASIRKLSTKIDICIKAV 611
           WEKKLYKEVKDEERLRVIYEKLCKKLKRLD+HGADSTKIDATHASIRKLSTKID+CIKA 
Sbjct: 601 WEKKLYKEVKDEERLRVIYEKLCKKLKRLDEHGADSTKIDATHASIRKLSTKIDVCIKAA 660

BLAST of Lcy12g013940 vs. TAIR 10
Match: AT1G21740.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 464.5 bits (1194), Expect = 1.3e-130
Identity = 332/714 (46.50%), Postives = 428/714 (59.94%), Query Frame = 0

Query: 1   MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSLTDIGEAIRKFVDEEI 60
           MGCGGSKVD+  LV LCRERK+LIKAAS HR ALAAAH++YFQSL D+G++I++FVDEE+
Sbjct: 1   MGCGGSKVDDQPLVILCRERKQLIKAASHHRCALAAAHLSYFQSLCDVGDSIKRFVDEEL 60

Query: 61  VISGADSSSTHGSPVLTLPSDEGKGKRKKPKSGEKHINSSSSSSILHSVSISHEPSPHED 120
           V+ G  SSS+  SPVLTLPSDEGK          KH  SSSS+S+ HSV    +    ED
Sbjct: 61  VLVGTSSSSSPDSPVLTLPSDEGK--------PHKHKISSSSTSVSHSVIEDDDEGEGED 120

Query: 121 EIDGSHLHLSSGS--DSESEHHSS---GHIQIEDSPVQDE-------------------- 180
           +    HLHLSSGS  DS SE  S    GHI IE SP   E                    
Sbjct: 121 Q----HLHLSSGSELDSGSESGSDDSLGHIHIETSPKVKEKETRLPENYQPGYQSGYQSG 180

Query: 181 -------GYSHPP-----YAYPPRDW-------------SPPTNTYAYYMQRSTTPAKTV 240
                  GY + P     Y YP   W             +P  N   Y+M++S  P++ V
Sbjct: 181 YQPGFTPGYQYQPGYSAGYQYPVEGWGFMGENPNLNPYPNPNQNPGMYFMKKSVQPSRPV 240

Query: 241 MYDGPETHTASDGQW-PDPSYSYPQ-YPQYENGGFYGFSMGSQPDYNLYNQQPRRPATP- 300
           ++  PE H   +GQW P+    Y   YP   N G++G           Y +Q R P +P 
Sbjct: 241 VFQ-PENHRVENGQWLPENGVGYSNYYPGNANTGYFG-----------YPEQRREPPSPV 300

Query: 301 ---PPPPSPPKVSAWDFMNVFDGYDNVYQDYNAGNRYGYG-----------SIQSSPDSN 360
              P PPSPP++S+WDF+NVFD YD  Y     G   G G           S  SSPDS 
Sbjct: 301 RPTPAPPSPPRISSWDFLNVFDTYD--YNRAGGGESSGAGAGFFPAMGGGKSNSSSPDSR 360

Query: 361 EVREREGIPELEDETEPE-------------ALKEIKER----KKLNVETMNTNMNSGEG 420
           EVREREGIPELE+ETE E              ++++KE+     +++   +    +SGEG
Sbjct: 361 EVREREGIPELEEETEQEVIFGQTFKHMKRKGIEKVKEQHRQENEIHERKIKKRGDSGEG 420

Query: 421 TSKF--VTPHSSEDSTKSVPLPNSGSSPISKE-------KGIDNSPD----TNVSKKS-- 480
           TS+   V   ++E S  S  + +  SS    E       +G  +S D      V+ KS  
Sbjct: 421 TSRAVPVVERATESSFGSKTVSSFSSSEEESEFHHVNDGEGKSSSNDLGGHETVATKSVG 480

Query: 481 -EQEEPVEKKELSFEIEE--TSTLDIESSKKSNLATFTAYGTRDLQEVVNEIKDEFEAAS 540
             +EE V KK +SFE++E  T++ D+ESSK S+L+  + + TRDL+EVV EIK EFE AS
Sbjct: 481 EVEEEYVRKKGVSFELDENVTTSFDVESSKISSLSALSVHATRDLREVVKEIKSEFEVAS 540

Query: 541 NHGKEVAMLLEVGRLPYRSKIAVLKVILSRIQYLVAPSSAS--SQP-PLIWLDPKTVKRA 600
           +HGKEVA+LLEV +LPY+ K + LKVI SRI YLVAPS+ S  SQP P I L  + +K A
Sbjct: 541 SHGKEVAVLLEVSKLPYQQKSSGLKVIFSRIMYLVAPSTVSSRSQPQPSIRLTSRILKIA 600

Query: 601 KAYSGSGSPGNELDLKSGSLSSTLEKLYVWEKKLYKEVKDEERLRVIYEKLCKKLKRLDD 610
           K+Y+G     +  +  +G+LS+TLE+LY WEKKLYKEVKDEE+LRV+YE+ C+ LK+LD 
Sbjct: 601 KSYNGQ----DVREGLTGNLSATLEQLYAWEKKLYKEVKDEEKLRVVYEEKCRTLKKLDS 660

BLAST of Lcy12g013940 vs. TAIR 10
Match: AT1G77500.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 461.1 bits (1185), Expect = 1.5e-129
Identity = 322/679 (47.42%), Postives = 402/679 (59.20%), Query Frame = 0

Query: 1   MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSLTDIGEAIRKFVDEEI 60
           MGCGGSKVD   +V LCRERKEL+KAAS HR ALA AH+TYFQSL+D+GEAI++FVD+E 
Sbjct: 1   MGCGGSKVDNQPIVILCRERKELLKAASYHRSALAVAHLTYFQSLSDVGEAIQRFVDDE- 60

Query: 61  VISGADSSSTHGSPVLTLPSDEGKGKRKKPKSGEKHINSSSSSSILHSVSISHEPSPHED 120
           V++G  SSS+  SPVLTLPSDEGK  +       K I+ SS++SI HSV         ED
Sbjct: 61  VVAGFSSSSSPDSPVLTLPSDEGKPTK------HKRISPSSTTSISHSV------IEEED 120

Query: 121 EIDGSHLHLSSGSDSESEHHSSGHIQIEDSPVQDEGYSHPPYAYPPRDWSPPT------- 180
             D SHLHLSSGS+SESE  S  HIQI  +P Q+      P  Y P +++PP        
Sbjct: 121 TDDDSHLHLSSGSESESEVGSKDHIQITSTPEQERSTESFPSGYHPTNYAPPVYPPGYPP 180

Query: 181 -----------------------------NTYAYYMQRSTTPAKTVMYDGPETHTASD-G 240
                                        N   YYM++S   ++  ++  PE H   +  
Sbjct: 181 GYPFSYPTGYSSGNYQPGYPNYTGENPYGNRGMYYMKKSAPQSRPFIFQ-PENHRVEENA 240

Query: 241 QWPDPSYSYPQYPQYENGGFYGFSMGSQPDYNLYNQQPRRPATPPPPPSPPKVSAWDFMN 300
           QWP  S        + N G                   RR  +P PPPSPP VS WDF+N
Sbjct: 241 QWPSDS-------GFRNTGV-----------------QRRSPSPLPPPSPPTVSTWDFLN 300

Query: 301 VFDGYDNVYQDYNAGNRY--GYGSIQSSPDSNEVREREGIPELEDETEPEALKEIKER-K 360
           VFD YD       A   Y  G  SI SSPDS EVREREGIPELE+ TE E +K++  R K
Sbjct: 301 VFDTYDYSNARSRASGYYPMGMASISSSPDSKEVREREGIPELEEVTEQEVIKQVYRRPK 360

Query: 361 KLNVETM-------------NTNMNSGEG-----------TSKFVTPHSSEDSTKSVPLP 420
           +  +E +               N+N  E             S+ ++  S  D        
Sbjct: 361 RPGLEKVKEHRDEHKHNVFPERNINKREVPMPEQVTESSLDSETISSFSGSDVESEFHYV 420

Query: 421 NSGSSPISKEKGIDNSPDTNVSKKSEQEEPVEKKELSFEIEE---TSTLDIESSKKSNLA 480
           N G    S    I +   T  S++ E E+   KK +SFE+EE   TS+ D+ESSK S+L+
Sbjct: 421 NGGEGKSSSISSISHGAGTKSSREVE-EQYGRKKGVSFELEETTSTSSFDVESSKISSLS 480

Query: 481 TFTAYGTRDLQEVVNEIKDEFEAASNHGKEVAMLLEVGRLPYRSKIAVLKVILSRIQYLV 540
           + + + TRDL+EVV EIK EFE AS+ GKEVA+LLEVG+LPY+ K   +KVILSRI YLV
Sbjct: 481 SLSIHATRDLREVVKEIKSEFEIASSCGKEVALLLEVGKLPYQHKNNGVKVILSRIMYLV 540

Query: 541 APS--SASSQPPL-IWLDPKTVKRAKAYSGSGSPGNELDLKSGSLSSTLEKLYVWEKKLY 600
           APS  S+ SQP L I L  +T K AK+Y+G    G      +G+LSSTLEKLY WEKKLY
Sbjct: 541 APSTRSSHSQPRLSIRLTSRTRKMAKSYNGQDVNGG----FNGNLSSTLEKLYAWEKKLY 600

Query: 601 KEVKDEERLRVIYEKLCKKLKRLDDHGADSTKIDATHASIRKLSTKIDICIKAVDAISSR 610
           KEVKDEE+LR IYE+ C++LK++D HGA+S KIDAT A+IRKL TKID+CI++VD+ISSR
Sbjct: 601 KEVKDEEKLRAIYEEKCRRLKKMDSHGAESIKIDATRAAIRKLLTKIDVCIRSVDSISSR 636

BLAST of Lcy12g013940 vs. TAIR 10
Match: AT4G35240.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 237.7 bits (605), Expect = 2.7e-62
Identity = 217/637 (34.07%), Postives = 300/637 (47.10%), Query Frame = 0

Query: 1   MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSLTDIGEAIRKFVDEEI 60
           MGC  SK+D+L  V LCRER   ++AA   RYALA +HV Y  SL +IG ++  F++   
Sbjct: 1   MGCTSSKLDDLPAVALCRERCAFLEAAIHQRYALAESHVAYTHSLREIGHSLHLFINHHH 60

Query: 61  VISGADSSSTHGSPVLTLP-------SDEGKGKRKKPKSGEKH--INSSSSSSILHSVSI 120
               +  ++   SP L LP        DE     KK K    H   N + S S   S  +
Sbjct: 61  RFVASGGANVGDSPRLNLPPQRKGDLDDEATNSPKKQKLASSHHNHNHAHSGSGSDSGHL 120

Query: 121 SHEPSPHEDEIDGSHLHLSSGSDSESEHHSSGHIQI-EDSPV-----QDEGYSHPPYAYP 180
             +    EDE D   L L S       HH  G+  I E +P+     Q  GY + PY  P
Sbjct: 121 EFDSDSDEDEEDDDDLDLDSLHHHSPPHHHLGNFPIPESAPMGGYMEQQPGYIN-PYPNP 180

Query: 181 PRDWSPPTNTYAY----YMQRSTTPAKTVMYDGPETHTASDGQWPDPSYSYPQYPQYENG 240
                P +   +Y    YM+  + P   V    P   +       + S SYP  PQ    
Sbjct: 181 DMMGHPYSGGGSYMHMNYMKNKSMPPSVVYEQRPT--SPQRVYIGESSSSYPYPPQNSYF 240

Query: 241 GFYGFSMGSQPDYNLYNQQPRRPAT---PPPPPSPPKVSAWDFMNVFDGYDNVYQDYNAG 300
           G+     G  P Y   +      A    PPPPPSPP+ + WDF+N FD Y          
Sbjct: 241 GYSNPVPGPGPGYYGSSSASTTAAATKPPPPPPSPPRSNGWDFLNPFDTY---------- 300

Query: 301 NRYGYGSIQSSPDSNEVREREGIPELE-DETEPEALKEIKERKKLNVETMNTNMNSGEGT 360
               Y     S DS E+RE EGIP+LE D++  E +KE+  + K            G   
Sbjct: 301 ----YPPYTPSRDSRELREEEGIPDLEDDDSHYEVVKEVYGKPKFAA--------GGGHQ 360

Query: 361 SKFVTPHSSEDSTKSVPLPNSGSSPISKEKGIDNSPDTNVSKKSEQEEPVEKKELSFEIE 420
                 H   + + S PL  SG+S      G  +  D + + +S     VEK+ + +E+ 
Sbjct: 361 PNPAAVHMMREESPSPPLDKSGAS----TSGGGDVGDAS-AYQSRPSVSVEKEGMEYEVH 420

Query: 421 ETSTLDIESSKKSNLATFT-----AYGTRDLQEVVNEIKDEFEAASNHGKEVAMLLEVGR 480
                 +E  ++ + AT T       G R + EV  EI+++F  A+  G E+A LLEVG+
Sbjct: 421 VVEKKVVEDEERRSNATATRGGGGGGGPRAVPEVAKEIENQFVKAAESGSEIAKLLEVGK 480

Query: 481 LPYRSKIAVLKVILSRIQYLVAPSSASSQPPLIWLDPKTVKRAKAYSGSGSPGNELDLKS 540
            PY  K A  K++      L + S  +S      + P T    +          EL  +S
Sbjct: 481 HPYGRKHAASKMLHGVTPSLPSTSGGTSSSAAAAVVPPTYADIE---------EELASRS 540

Query: 541 GSLSSTLEKLYVWEKKLYKEVKDEERLRVIYEKLCKKLKRLDDHGADSTKIDATHASIRK 600
            +LSSTL KL++WEKKLY EVK EE+LR+ +EK  +KLKRLD  GA++ K+D T   +R 
Sbjct: 541 RNLSSTLHKLHLWEKKLYHEVKAEEKLRLAHEKKLRKLKRLDQRGAEAIKVDKTRKLVRD 598

Query: 601 LSTKIDICIKAVDAISSRIHKLRDEELQPQLTELIHG 610
           +STKI I I+ VD IS  I+K+RDE+L PQL  LI G
Sbjct: 601 MSTKIRIAIQVVDKISVTINKIRDEDLWPQLNALIQG 598

BLAST of Lcy12g013940 vs. TAIR 10
Match: AT4G35240.2 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 237.7 bits (605), Expect = 2.7e-62
Identity = 217/637 (34.07%), Postives = 300/637 (47.10%), Query Frame = 0

Query: 1   MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSLTDIGEAIRKFVDEEI 60
           MGC  SK+D+L  V LCRER   ++AA   RYALA +HV Y  SL +IG ++  F++   
Sbjct: 1   MGCTSSKLDDLPAVALCRERCAFLEAAIHQRYALAESHVAYTHSLREIGHSLHLFINHHH 60

Query: 61  VISGADSSSTHGSPVLTLP-------SDEGKGKRKKPKSGEKH--INSSSSSSILHSVSI 120
               +  ++   SP L LP        DE     KK K    H   N + S S   S  +
Sbjct: 61  RFVASGGANVGDSPRLNLPPQRKGDLDDEATNSPKKQKLASSHHNHNHAHSGSGSDSGHL 120

Query: 121 SHEPSPHEDEIDGSHLHLSSGSDSESEHHSSGHIQI-EDSPV-----QDEGYSHPPYAYP 180
             +    EDE D   L L S       HH  G+  I E +P+     Q  GY + PY  P
Sbjct: 121 EFDSDSDEDEEDDDDLDLDSLHHHSPPHHHLGNFPIPESAPMGGYMEQQPGYIN-PYPNP 180

Query: 181 PRDWSPPTNTYAY----YMQRSTTPAKTVMYDGPETHTASDGQWPDPSYSYPQYPQYENG 240
                P +   +Y    YM+  + P   V    P   +       + S SYP  PQ    
Sbjct: 181 DMMGHPYSGGGSYMHMNYMKNKSMPPSVVYEQRPT--SPQRVYIGESSSSYPYPPQNSYF 240

Query: 241 GFYGFSMGSQPDYNLYNQQPRRPAT---PPPPPSPPKVSAWDFMNVFDGYDNVYQDYNAG 300
           G+     G  P Y   +      A    PPPPPSPP+ + WDF+N FD Y          
Sbjct: 241 GYSNPVPGPGPGYYGSSSASTTAAATKPPPPPPSPPRSNGWDFLNPFDTY---------- 300

Query: 301 NRYGYGSIQSSPDSNEVREREGIPELE-DETEPEALKEIKERKKLNVETMNTNMNSGEGT 360
               Y     S DS E+RE EGIP+LE D++  E +KE+  + K            G   
Sbjct: 301 ----YPPYTPSRDSRELREEEGIPDLEDDDSHYEVVKEVYGKPKFAA--------GGGHQ 360

Query: 361 SKFVTPHSSEDSTKSVPLPNSGSSPISKEKGIDNSPDTNVSKKSEQEEPVEKKELSFEIE 420
                 H   + + S PL  SG+S      G  +  D + + +S     VEK+ + +E+ 
Sbjct: 361 PNPAAVHMMREESPSPPLDKSGAS----TSGGGDVGDAS-AYQSRPSVSVEKEGMEYEVH 420

Query: 421 ETSTLDIESSKKSNLATFT-----AYGTRDLQEVVNEIKDEFEAASNHGKEVAMLLEVGR 480
                 +E  ++ + AT T       G R + EV  EI+++F  A+  G E+A LLEVG+
Sbjct: 421 VVEKKVVEDEERRSNATATRGGGGGGGPRAVPEVAKEIENQFVKAAESGSEIAKLLEVGK 480

Query: 481 LPYRSKIAVLKVILSRIQYLVAPSSASSQPPLIWLDPKTVKRAKAYSGSGSPGNELDLKS 540
            PY  K A  K++      L + S  +S      + P T    +          EL  +S
Sbjct: 481 HPYGRKHAASKMLHGVTPSLPSTSGGTSSSAAAAVVPPTYADIE---------EELASRS 540

Query: 541 GSLSSTLEKLYVWEKKLYKEVKDEERLRVIYEKLCKKLKRLDDHGADSTKIDATHASIRK 600
            +LSSTL KL++WEKKLY EVK EE+LR+ +EK  +KLKRLD  GA++ K+D T   +R 
Sbjct: 541 RNLSSTLHKLHLWEKKLYHEVKAEEKLRLAHEKKLRKLKRLDQRGAEAIKVDKTRKLVRD 598

Query: 601 LSTKIDICIKAVDAISSRIHKLRDEELQPQLTELIHG 610
           +STKI I I+ VD IS  I+K+RDE+L PQL  LI G
Sbjct: 601 MSTKIRIAIQVVDKISVTINKIRDEDLWPQLNALIQG 598

BLAST of Lcy12g013940 vs. TAIR 10
Match: AT2G17110.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 209.5 bits (532), Expect = 7.8e-54
Identity = 208/629 (33.07%), Postives = 283/629 (44.99%), Query Frame = 0

Query: 1   MGCGGSKVDELQLVTLCRERKELIKAASRHRYALAAAHVTYFQSLTDIGEAIRKFVDEEI 60
           MGC  SK+D+L  V LCR+R   ++AA   RYAL+ AHV+Y QSL  I  ++ +F++   
Sbjct: 1   MGCSTSKLDDLPAVALCRDRCSFLEAAIHQRYALSEAHVSYTQSLKAISHSLHQFINHHH 60

Query: 61  VISGADSSSTHGSPVLTLPSDEGKGKRKKPK--SGEKHINSSSSSSILHSVSISHEPSPH 120
             + +DS                  K+ KPK  SG  H++  S S            S  
Sbjct: 61  RYNDSDSP-----------------KKAKPKMDSGSGHLDFDSDSD-----------SDD 120

Query: 121 EDEIDGSHLHLSSGSDSESEHHSSGHIQIEDSPVQDEGYSHPPYAYPPRDWSPPTNTYAY 180
           +D+ID  H        S   HH   H++ +DS                   +P +  +  
Sbjct: 121 DDDIDSLH--------SSPLHH---HLEDDDS-------------------NPKSYLHMN 180

Query: 181 YMQRSTTPAKTVMYDGPETHTASDGQWPDPSYSYPQYPQYENGGFYG-FSMGSQPDYNLY 240
           YM+ S  P                     PS  Y Q P       +G  S  S  +YN Y
Sbjct: 181 YMKNSYMP---------------------PSLVYEQRPSSPQRVHFGESSSSSTSEYNPY 240

Query: 241 NQQPRRPATPPPPPSPPKVSAWDFMNVFDGYDNVYQDYNAGNRYGYGSIQSSPDSNEVRE 300
                    PPPPPSPP+   WDF++ FD Y              Y     S D+ E+R+
Sbjct: 241 LNSNYGSKLPPPPPSPPREKVWDFLDPFDTY--------------YTPYTPSRDTRELRD 300

Query: 301 REGIPELEDETEPEALKEIKERKKLNVETMNTNMNSGEGTSKFVTPHSSEDSTKSVPLPN 360
             G+P+LE+  +   +KE+                   G  KFV   S E+     PL N
Sbjct: 301 ELGVPDLEE--DDVVVKEV------------------HGKQKFVAAVSVEE-----PLGN 360

Query: 361 SGSSPI------SKEKGIDNSPDTNVSKKSEQEEP--VEKKELSFEIEETSTLDIESSKK 420
           SG+S         K       P  +V K+  + E   VEKK     I E S  D     K
Sbjct: 361 SGASTSGGGGGGGKASLYQTRPSVSVEKEEMEHEVHIVEKK-----IVEDSGGDEVRKSK 420

Query: 421 SNLATFTAYGTRDLQEVVNEIKDEFEAASNHGKEVAMLLEVGRLPYRSKIAVLKVILSRI 480
           + +A       R + EV  EI+ +F  A+  G E+A++LEVG+ PY  K    K +    
Sbjct: 421 AAVARGGGGVRRGVPEVAKEIEAQFLRAAESGNEIAVMLEVGKHPYGRKNVSSKKLYEGT 480

Query: 481 QYLVAPSSASSQPPLIWLDPKTVKRAKAYSGSGSPG-------NELDLKSGSLSSTLEKL 540
                 SSA S          T K+AKA + S            EL LKS +LSSTL KL
Sbjct: 481 PSPSVVSSAQS---------STSKKAKAEASSSVTAPTYADIEAELALKSRNLSSTLHKL 497

Query: 541 YVWEKKLYKEVKDEERLRVIYEKLCKKLKRLDDHGADSTKIDATHASIRKLSTKIDICIK 600
           ++WEKKLY EVK EE++RV +EK  +KLKR+D+ GA++ K+D+T   +R LSTKI I I+
Sbjct: 541 HLWEKKLYDEVKAEEKMRVNHEKKLRKLKRMDERGAENQKVDSTRKLVRSLSTKIRIAIQ 497

Query: 601 AVDAISSRIHKLRDEELQPQLTELIHGYS 612
            VD IS  I+K+RDEEL  QL ELI G S
Sbjct: 601 VVDKISVTINKIRDEELWLQLNELIQGLS 497