Lcy12g013550 (gene) Sponge gourd (P93075) v1

BLAST of Lcy12g013550 vs. ExPASy Swiss-Prot
Match: O65709 (Long-chain-alcohol oxidase FAO4A OS=Arabidopsis thaliana OX=3702 GN=FAO4A PE=3 SV=2)

HSP 1 Score: 866.3 bits (2237), Expect = 2.7e-250
Identity = 438/744 (58.87%), Postives = 560/744 (75.27%), Query Frame = 0

Query: 60  MESLAALCDTFLPS-----VAAADRSAAVFFETSASTADTPERVGEMIKERVRHPKKWLA 119
           MESL A+CDTF+ S     V   D   A +F  SAS   TP+RV  ++ ER+ HPKKW+ 
Sbjct: 1   MESLVAICDTFISSIDDSGVGHVDDCVAGYFSASASQTGTPDRVARLMSERLHHPKKWIL 60

Query: 120 RLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFLLRMFFR 179
           R  LW+LSTW G+ ++CG +S + +FPYF+RF ++ ++RRE+I+++WS SYF LLRM FR
Sbjct: 61  RAGLWLLSTWIGSLVLCGWRSFTGEFPYFRRFCRLPEKRREEILLNWSSSYFSLLRMLFR 120

Query: 180 TVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKENGRSREVEEDE 239
           T+K+   L FFTQVD+K  N++W AI Y G  P             L E  + ++     
Sbjct: 121 TIKLISALVFFTQVDEKGRNLAWKAIGYNGPSPD-----HSDHEVELNEEKKKKK----P 180

Query: 240 EELYGPLFKGIVNLEHQQVAAAEALRKFGFSVTLLSPRTKASS---PSMIIKCDAVVVGS 299
           EE++GPL+ GIV+L+  + A  + L   GF+V+     T  SS   P M I+CDAVVVGS
Sbjct: 181 EEIFGPLYNGIVDLKSPREAVEKKLAGRGFAVSNQKRNTNGSSISDPVMKIQCDAVVVGS 240

Query: 300 GSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDNMNVLVL 359
           GSGGGV AGVLA AGYKVLV+E G+Y AR+ LSLLEGQAM++MYLS GL+AT + NV++L
Sbjct: 241 GSGGGVAAGVLAKAGYKVLVIESGNYYARSKLSLLEGQAMDDMYLSGGLLATSDTNVVIL 300

Query: 360 AGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGVQSEIDN 419
           AGSTVGGGST+NWSASI+TP HV +EW+++ +LE+F S LY+EAM+VVCK+MGVQ     
Sbjct: 301 AGSTVGGGSTINWSASIKTPEHVMKEWAEKSKLEMFGSDLYREAMDVVCKRMGVQCGFVE 360

Query: 420 EGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGN 479
           EGFNN VLRKGCE+LG  V NIPRN+PSDHYCG+CCLGCK G+K+GT+ETWLVDLV S N
Sbjct: 361 EGFNNEVLRKGCEKLGLPVKNIPRNAPSDHYCGFCCLGCKKGQKQGTSETWLVDLVESDN 420

Query: 480 GVILPGCEAIDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVTVVACGALSTPP 539
           G+ILPGC+A ++  + + G ++ +A GVAF FG     +++++VES+VT+VACGAL TP 
Sbjct: 421 GLILPGCQATEVMYDCEQG-KKKKATGVAFAFG-----EEIYVVESRVTIVACGALRTPH 480

Query: 540 LLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAMCNIGSEPD 599
           LL++SGL+N NIG+NL LHPV MAWG FP E DK WPE+ KKSYEGGIMTAM ++  E +
Sbjct: 481 LLKRSGLKNSNIGRNLCLHPVVMAWGWFP-EEDK-WPEKKKKSYEGGIMTAMSSVVIE-E 540

Query: 600 QEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDKGSGTIKSP 659
               YG  VIQTPALHPG+FS ++PW S  + K RM KFSRTAH+FAL RDKG+GTI S 
Sbjct: 541 THSSYGEMVIQTPALHPGMFSGIIPWTSSKDFKTRMLKFSRTAHIFALLRDKGTGTIDSK 600

Query: 660 SSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYEKFVKE 719
           +   YN++D DEE+L+ GLE+ LKILAAAGAEEIGTHH++GRS+NV+  S  E E+FV+E
Sbjct: 601 TYIDYNLNDEDEESLKNGLERVLKILAAAGAEEIGTHHSEGRSLNVRTASSLEIERFVRE 660

Query: 720 ESSRPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIADSSVFPTALGVN 779
           ESS+PLKDL   ICSAHQMGSCRMGIR ++S V   GETWEVE LF+AD+SVFPTALGVN
Sbjct: 661 ESSKPLKDLSGQICSAHQMGSCRMGIRPEESAVRPTGETWEVERLFVADTSVFPTALGVN 720

Query: 780 PMVTVQAIAYCTAQSVLETLKRRR 796
           PMVTVQ+IAYC   +V++ LK+++
Sbjct: 721 PMVTVQSIAYCIGLNVVDVLKKKK 726

BLAST of Lcy12g013550 vs. ExPASy Swiss-Prot
Match: B5WWZ8 (Long-chain-alcohol oxidase FAO1 OS=Lotus japonicus OX=34305 GN=FAO1 PE=1 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 8.3e-159
Identity = 315/765 (41.18%), Postives = 456/765 (59.61%), Query Frame = 0

Query: 49  NSNNSSLSAAEMESLAALCDTFLPSVA-----------AADRSAAVFFETSASTADTPER 108
           N      S++EMESLA++C+  LP +             +      F+ TSAS    P  
Sbjct: 16  NKFRQGFSSSEMESLASICEVILPPLPMDALKIKKHDDVSIEDVEFFWNTSASHYPIPHE 75

Query: 109 VGEMIKERVRHPKKWLARLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQI 168
           V E++ +R       L R+ LW+L+T  GT+++CG    S K+P+   FS +S ++RE++
Sbjct: 76  VAEILSKRSLIEAVILIRVVLWLLATRLGTFLLCGLLCFSEKWPFINNFSSMSLKKRERV 135

Query: 169 VVSWSLSYFFL-LRMFFRTVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQS 228
           +  W  + F   +R+ F  +K+ CL A+FT VD+  +N +W AI Y         +    
Sbjct: 136 MQWWLKNRFITPIRLAFAYLKVLCLFAYFTWVDENGDNPAWKAIGY---------EVSAD 195

Query: 229 EASNLKENGRSREVEEDEEELYGPLFKGIVNLEHQ-QVAAAEALRKFGFSVTLLSPRTKA 288
           E      NGR  E             KGIV   H+      ++L + G +VTL      +
Sbjct: 196 EKLPNSSNGRLLE-------------KGIVETMHETNSTLQQSLTEKGLNVTL-----DS 255

Query: 289 SSPSMIIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMY 348
            +  + +KCDA+VVGSG GGGV A VL+ AG+KV+VLEKG+Y A  + S LEG +M+++Y
Sbjct: 256 RNNILKVKCDAIVVGSGCGGGVAASVLSGAGHKVVVLEKGNYFAPRDYSSLEGPSMDQLY 315

Query: 349 LSSGLVATDNMNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEA 408
            + G++A+ +  +L+LAGSTVGGGS VNWSA I+TP  V +EWS+ + L LFS+S Y  A
Sbjct: 316 ETGGILASVDSRILLLAGSTVGGGSAVNWSACIKTPHEVLKEWSENHNLSLFSTSEYLSA 375

Query: 409 MEVVCKKMGVQSEIDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRK 468
           ME VC+++GV     +EGF N VLRKGC+ LG  V  +PRNS  +H+CG C  GC  G K
Sbjct: 376 METVCERIGVTESCTHEGFQNQVLRKGCQNLGLKVDYVPRNSSGNHFCGSCGYGCPKGEK 435

Query: 469 KGTAETWLVDLVRSGNGVILPGCEAID-IRQEKKDGSERNRAKGVAFEFGCGSGAKQMFL 528
           +GT +TWLVD V +G  VI+ GC+A   + +  ++GS R +           S       
Sbjct: 436 QGTQDTWLVDAVENG-AVIITGCKAERFLLENNRNGSGRKKKCLGVLAKALSSRVTMKLQ 495

Query: 529 VESKVTVVACGALSTPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKS 588
           +E+KVT+ A GAL TPPL+  SGL+N NIGKNLHLHPV M WG+FP  T     E   K 
Sbjct: 496 IEAKVTISAGGALLTPPLMISSGLKNKNIGKNLHLHPVLMTWGYFPESTS----ELKGKI 555

Query: 589 YEGGIMTAMCNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTA 648
           +EGGI+T++  + S          A+I+TP+L PG F+ L PW SG + K+RM  + RTA
Sbjct: 556 FEGGIITSVHKVPSRDSNSYSDTRAIIETPSLGPGSFASLCPWESGLDFKERMLNYPRTA 615

Query: 649 HVFALARDKGSGTIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRS 708
           H+  + RDK  G + +    SY +   D+EN++ GL++AL+IL AAGA E+GTH + G+ 
Sbjct: 616 HLITIIRDKACGQVTTEGRVSYKLSAFDKENMKCGLQQALRILKAAGAVEVGTHRSDGQR 675

Query: 709 INVKKVSYREYEKFVKEESSRPLKDLMTP------ICSAHQMGSCRMGIRAKDSVVNQRG 768
           +   +VS  E  +F+  +S  P++  ++P        SAHQMGSCRMG+  K+  V++ G
Sbjct: 676 VKCGEVSENEMAEFI--DSVCPMEGALSPGENWNIYTSAHQMGSCRMGMNEKEGAVDENG 735

Query: 769 ETWEVEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSVLETLKR 794
           E+WE +GLF+ D+S+ PTA+GVNPM+T+Q+ AYC +  V++ LKR
Sbjct: 736 ESWEAQGLFVCDASLLPTAVGVNPMITIQSTAYCISNRVVDYLKR 746

BLAST of Lcy12g013550 vs. ExPASy Swiss-Prot
Match: B5WWZ9 (Long-chain-alcohol oxidase FAO2 OS=Lotus japonicus OX=34305 GN=FAO2 PE=2 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 3.7e-151
Identity = 303/761 (39.82%), Postives = 452/761 (59.40%), Query Frame = 0

Query: 55  LSAAEMESLAALCDTFLPS--------VAAADRSAAVFFETSASTADTPERVGEMIKERV 114
           LS+++M  +AA+C+   PS         ++ D++ + F+  S S A  P+   E++ +  
Sbjct: 27  LSSSQMHVIAAICEALFPSQPLDSQNNQSSVDKALSAFYTASGSQAPLPDEAAELLFKFN 86

Query: 115 RHPKKWLARLS--LWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLS 174
           R   + L+ +S  L +LS   GT ++CG   L  ++P+  +FS++   +RE+I+  WS  
Sbjct: 87  RSFPEALSLVSWVLLILSFRLGTLLLCGTLCLDWRWPFIHKFSEIPLEKREEILKRWSRE 146

Query: 175 YFFL-LRMFFRTVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKE 234
             ++ LR+ F   K+ C    F++ D   +N  W AI Y       +  TR+     L +
Sbjct: 147 KCWIPLRLVFVLTKLVCFYNLFSRADVNGHNPIWKAIGY-------QVDTRE----KLTQ 206

Query: 235 NGRSREVEEDEEELYGPLFKGIVNLEHQ-QVAAAEALRKFGFSVTLLSPRTKASSPSMII 294
             R             PL +G++   ++      ++L + G  VT              I
Sbjct: 207 KKR-------------PLQEGLIETMYETDSTLIQSLTEKGLEVT-----EDLEQNMYKI 266

Query: 295 KCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVA 354
           KCDAV+VGSG GGGV A VLAN+G+KV++LEKG Y    + S LEG +M E+Y S G++ 
Sbjct: 267 KCDAVIVGSGCGGGVAAAVLANSGHKVIILEKGEYFVSHDYSSLEGPSMNELYESGGILP 326

Query: 355 TDNMNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKK 414
           + +  +++LAGST+GG S +NWSA I+TP  V REWS+++++ LF+S  Y+ AM+ VC++
Sbjct: 327 SLDGKMMILAGSTLGGXSAINWSACIRTPDSVLREWSEKHKIPLFASPDYQSAMDTVCRR 386

Query: 415 MGVQSEIDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETW 474
           +GV    + E F N +LR+GC ++G  V  +  NS +DHYCG CC GC+ G KKGT  TW
Sbjct: 387 IGVTENCNKESFQNQILRQGCAKIGFKVEPVAINSSADHYCGSCCYGCRTGDKKGTESTW 446

Query: 475 LVDLVRSGNGVILPGCEAIDIRQEKKD---GSERNRAKGVAFEFGCGSGAKQMFLVESKV 534
           LVD V +G  VIL GC+A  +    KD   G++R    GV       S   +   +ESKV
Sbjct: 447 LVDAVGNG-AVILTGCKAEKLNFTLKDGDNGTKRKTCSGVIASATWRSKVTKKLQIESKV 506

Query: 535 TVVACGALSTPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGI 594
           T+ ACG+LSTPPL+  SGL+NPNIGKNLHLHP   AWG+FP +           +YEGGI
Sbjct: 507 TISACGSLSTPPLMISSGLKNPNIGKNLHLHPCQFAWGYFPEDMTNF----SGNNYEGGI 566

Query: 595 MTAMCNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFAL 654
           +T++  +  E          +I+ PAL PG FS L+PW+SG ++K+RM K++RTA++FAL
Sbjct: 567 ITSIHKVFEE--DSTSTPRIIIEAPALGPGSFSALVPWVSGLDVKERMVKYARTANLFAL 626

Query: 655 ARDKGSGTIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKK 714
            RD GSG +K+    SY +D +D E+LQ GL KAL+IL AAGA E+GT+ + G+ I  + 
Sbjct: 627 VRDHGSGEVKAEGRISYKLDKIDRESLQTGLRKALRILVAAGAVEVGTYRSDGQRIKCRG 686

Query: 715 VSYREYEKF------VKEESSRPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWEV 774
           +   + E+F      V   SSR   ++ T   SAHQM SCRM    ++  V++ GE+WE 
Sbjct: 687 IKESDLEEFLDSVRVVGGPSSR--NEVWTVFTSAHQMTSCRMSATEEEGAVDENGESWEA 746

Query: 775 EGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSVLETLKRR 795
           +GL++ D SV P+A+GVNPM+T+Q+ AYC A ++ E+LK++
Sbjct: 747 KGLYVCDGSVLPSAVGVNPMITIQSTAYCIASNIAESLKKQ 749

BLAST of Lcy12g013550 vs. ExPASy Swiss-Prot
Match: Q94BP3 (Long-chain-alcohol oxidase FAO4B OS=Arabidopsis thaliana OX=3702 GN=FAO4B PE=2 SV=2)

HSP 1 Score: 525.0 bits (1351), Expect = 1.5e-147
Identity = 305/755 (40.40%), Postives = 441/755 (58.41%), Query Frame = 0

Query: 52  NSSLSAAEMESLAALCDTFLPSVAAADRSAAVFFETSASTADT-PERVGEMIKERVRHPK 111
           N   S ++++SLA +C TFLP    +++ A   F  ++ST     + V EMI +  R   
Sbjct: 24  NHGFSPSQIQSLAVICQTFLPPETTSEQQAVNSFHVASSTQPPFTDEVAEMIVKNGRSEA 83

Query: 112 KWLARLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFLL- 171
             + R+ L +LS  +GT ++CG+  L   +P+  +FS++   +RE I+ +WS    FLL 
Sbjct: 84  VKVLRIILMILSFRFGTLLLCGSLCLDKSWPFVLKFSQLPLDKREAILRNWSRQSGFLLP 143

Query: 172 -RMFFRTVKIFCLLAFFTQVDDKMNNISWNAIDY--PGTDPQFKAKTRQSEASNLKENGR 231
            R+ F   K + L  FF+Q D+ + N +  AI Y   GT+                   R
Sbjct: 144 FRITFFLAKFYTLFYFFSQTDENLKNPALEAIGYCIDGTE-------------------R 203

Query: 232 SREVEEDEEELYGPLFKGIVNLEHQ-QVAAAEALRKFGFSVTLLSPRTKASSPSMIIKCD 291
           S   + + +E   PL KGI+   H+  V   ++L + G  V               I+CD
Sbjct: 204 SSNKKSEADEKRRPLEKGIIETMHESDVTITQSLTEKGVHVA-----RDDGDNVYRIRCD 263

Query: 292 AVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDN 351
           AVVVGSGSGGGV A  LA AG KVLVLEKG+Y    + S LE  +M E+Y   GL+ T +
Sbjct: 264 AVVVGSGSGGGVAAANLAKAGLKVLVLEKGNYFTAHDYSGLEVPSMLELYEKGGLLTTVD 323

Query: 352 MNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGV 411
              ++LAGS VGGG+ VNWSASI+TP HV +EWS+  +++ F S  Y+ AM+ V  ++GV
Sbjct: 324 GKFMLLAGSAVGGGTAVNWSASIRTPDHVLQEWSEGSKIKFFGSQEYQSAMDEVTIRIGV 383

Query: 412 QSEIDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVD 471
                  GF N VLRKGCE LG  V ++PRNSP DHYCG C  GC+ G K GT +TWLVD
Sbjct: 384 TERCVKHGFQNQVLRKGCERLGLQVESVPRNSPEDHYCGLCGYGCRAGAKNGTDQTWLVD 443

Query: 472 LVRSGNGVILPGCEAIDIRQEKKDGSERNRAKGVAFEFGCGSGAK--QMFLVESKVTVVA 531
            V +G  VIL G +A          S   R K     F    G K  + F++E++VTV +
Sbjct: 444 AVENG-AVILTGIKAERFVLVDNTSSSNERKKRCVGVFASSVGGKIGKKFIIEARVTVSS 503

Query: 532 CGALSTPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAM 591
            G+L TPPL+  SGL+NPNIG+NL LHPV M WG+FP E D    E   K YEGGI+T++
Sbjct: 504 AGSLLTPPLMLSSGLKNPNIGRNLKLHPVLMTWGYFP-EKDS---EFSGKMYEGGIITSV 563

Query: 592 CNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDK 651
            ++    D E G   A+++ P + P  ++ L PW+SG ++K+RM K+ RTAH+FAL RD 
Sbjct: 564 HHMN---DTESGC-KAILENPLIGPASYAGLSPWVSGPDLKERMIKYGRTAHLFALVRDL 623

Query: 652 GSGTIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYR 711
           GSG +   +  +Y     D ENL+ GL +AL++  AAGA E+GT+ + G+ +  + ++  
Sbjct: 624 GSGEVMMENEVTYRTTKKDRENLRAGLRQALRVSVAAGAVEVGTYRSDGQKMKCEAITKE 683

Query: 712 EYEKFVKEESS----RPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIA 771
             E+F+ E  +        +  T   SAHQMGSCRMG+ A++  +++ GE+WE EGLF+ 
Sbjct: 684 AMEEFLDEVDAVGGVGTKGEYWTTYFSAHQMGSCRMGVTAEEGALDENGESWEAEGLFVC 743

Query: 772 DSSVFPTALGVNPMVTVQAIAYCTAQSVLETLKRR 795
           D S+ P+A+GVNPM+T+Q+ AYC +  ++++L+ +
Sbjct: 744 DGSILPSAVGVNPMITIQSTAYCISSKIVDSLQNK 745

BLAST of Lcy12g013550 vs. ExPASy Swiss-Prot
Match: Q9LW56 (Long-chain-alcohol oxidase FAO3 OS=Arabidopsis thaliana OX=3702 GN=FAO3 PE=1 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 7.3e-147
Identity = 307/759 (40.45%), Postives = 447/759 (58.89%), Query Frame = 0

Query: 55  LSAAEMESLAALCDTFLPSVAAADRSAA-------------VFFETSASTADTPERVGEM 114
           ++ AEMESLA+ C+  LPSV       +              F+ TS S      +  E+
Sbjct: 15  ITVAEMESLASFCEAVLPSVQPPPEELSGEGDNHRNKEALRSFYSTSGSKTPVLRQSIEL 74

Query: 115 IKERVRHPKKWLARLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSW 174
           + +R         RL L++L+T  GT ++CG + L +++P+ ++FS++S  +RE+++   
Sbjct: 75  VTKRGTIEAYIATRLILFLLATRLGTLLICGTECLVSRWPFVEKFSELSLEKRERVLQKQ 134

Query: 175 SLSYFFL-LRMFFRTVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASN 234
             ++    +R  F  +K+  L  FF++V+    N +W AI Y   +P    + + SE  N
Sbjct: 135 FKNWILTPIRAAFVYIKVAFLFCFFSRVNPNGENPAWEAIGY-RVNPD---ENKPSETHN 194

Query: 235 LKENGRSREVEEDEEELYGPLFKGIV-NLEHQQVAAAEALRKFGFSVTLLSPRTKASSPS 294
            +                 PL KGIV  +E  +    E+L   G    L          +
Sbjct: 195 ER-----------------PLEKGIVETMEETEQTLLESLAHKGLEAVL-----DTEHDA 254

Query: 295 MIIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSG 354
           + IKCD VVVGSGSGGGV A VLA +G KV+VLEKGSY   +     EG  ++++Y + G
Sbjct: 255 IRIKCDVVVVGSGSGGGVAASVLAKSGLKVVVLEKGSYFTPSEHRPFEGPGLDKLYENGG 314

Query: 355 LVATDNMNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVV 414
           ++ + + + +VLAG+TVGGGS VNWSA I+TP  V +EWS++  + LF +  Y  AMEVV
Sbjct: 315 ILPSVDGSFMVLAGATVGGGSAVNWSACIKTPKSVLQEWSEDQNIPLFGTKEYLTAMEVV 374

Query: 415 CKKMGVQSEIDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTA 474
            K+MGV  + + E F N +LRKGCE LG +V N+PRNS   HYCG C  GC+ G KKG+ 
Sbjct: 375 WKRMGVTEKCELESFQNQILRKGCENLGFNVENVPRNSSESHYCGSCGYGCRQGDKKGSD 434

Query: 475 ETWLVDLVRSGNGVILPGCEAIDIRQEK---KDGSERNRAKGVAFEFGCGSGAKQMFLVE 534
            TWLVD V  G  VIL GC+A     EK     G ++ +  GV  +   G+ AK M  +E
Sbjct: 435 RTWLVDAVGHG-AVILTGCKAERFILEKNGSNKGGKQMKCLGVMAKSLNGNIAK-MLKIE 494

Query: 535 SKVTVVACGALSTPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYE 594
           +KVTV A GAL TPPL+  SGLRN NIGKNLHLHPV MAWG+FP + + S       SYE
Sbjct: 495 AKVTVSAGGALLTPPLMISSGLRNRNIGKNLHLHPVLMAWGYFP-DKESSNISFKGNSYE 554

Query: 595 GGIMTAMCNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHV 654
           GGI+T++  + SE  +      A+I+TP L PG FS+L PW SG ++K+RM ++SRTA +
Sbjct: 555 GGIITSVSKVLSEDSEV----RAIIETPQLGPGSFSVLTPWTSGLDMKKRMARYSRTASL 614

Query: 655 FALARDKGSGTIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSIN 714
             + RD+GSG +K+    +Y +D  D +NL+ GL ++L+IL AAGAEE+GTH + G+ + 
Sbjct: 615 ITIVRDRGSGEVKTEGRINYTVDKTDRDNLKAGLRESLRILIAAGAEEVGTHRSDGQRLI 674

Query: 715 VKKVSYREYEKFV----KEESSRPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWE 774
            K V+    ++F+     EE ++ + +      SAHQMGSCR+G   K+  ++  GE+WE
Sbjct: 675 CKGVNENSIQEFLDSVSTEEGAKGMTEKWNVYSSAHQMGSCRIGENEKEGAIDLNGESWE 734

Query: 775 VEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSVLETL 792
            E LF+ D+S  P+A+GVNPM+TV + AYC +  + +++
Sbjct: 735 AEKLFVCDASALPSAVGVNPMITVMSTAYCISTRIAKSM 740

BLAST of Lcy12g013550 vs. ExPASy TrEMBL
Match: A0A6J1FFC0 (Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111443434 PE=3 SV=1)

HSP 1 Score: 1387.9 bits (3591), Expect = 0.0e+00
Identity = 699/795 (87.92%), Postives = 737/795 (92.70%), Query Frame = 0

Query: 1   MGYFDATAAASCGGDGEAKRQLDHCVIDLHGGADRLHDGAPAGISQHANSNNSSLSAAEM 60
           MGYF   AAASCGGDGE+K QL  CVI+L+GGAD LHDG PAGISQHAN  N SLSAAEM
Sbjct: 12  MGYFG--AAASCGGDGESKPQLHQCVIELNGGADSLHDGGPAGISQHANF-NYSLSAAEM 71

Query: 61  ESLAALCDTFLPSVAAADRSAAVFFETSASTADTPERVGEMIKERVRHPKKWLARLSLWM 120
           ESLAALCDTFLPSVAA D SA+ FFETSAS A TPERVG MIKERVRHPKKWL R+SLWM
Sbjct: 72  ESLAALCDTFLPSVAATDGSASEFFETSASAAGTPERVGGMIKERVRHPKKWLMRVSLWM 131

Query: 121 LSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFLLRMFFRTVKIFC 180
           LSTWYGTYI+CGN SLSN FPYFQRFSKVSQ++REQ+VVSWSLSYF LLRMFFRT+KI C
Sbjct: 132 LSTWYGTYILCGNGSLSNHFPYFQRFSKVSQKKREQVVVSWSLSYFLLLRMFFRTIKILC 191

Query: 181 LLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKENGRSREVEEDEEELYGP 240
            LAFFTQVD++MNNISW AIDYPG+DPQFKAK RQSE  NLKE  RSRE+ E+EEELYGP
Sbjct: 192 HLAFFTQVDEEMNNISWKAIDYPGSDPQFKAKARQSEEWNLKEKVRSREIVEEEEELYGP 251

Query: 241 LFKGIVNLEHQQVAAAEALRKFGFSVTLLSPRTKASSPSMIIKCDAVVVGSGSGGGVVAG 300
           LFKGIVNLEHQQVA   ALRKFGF VTLLSP  K+ SPSMIIKCDAVVVGSGSGGGVVAG
Sbjct: 252 LFKGIVNLEHQQVAVG-ALRKFGFPVTLLSPSIKSPSPSMIIKCDAVVVGSGSGGGVVAG 311

Query: 301 VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDNMNVLVLAGSTVGGGS 360
           VLA+AGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGLVATDNMNVL+LAGSTVGGGS
Sbjct: 312 VLASAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNVLILAGSTVGGGS 371

Query: 361 TVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGVQSEIDNEGFNNAVLR 420
           TVNWSASIQTPSHVTREWSDEYELELF S+LYKEA+EVVCK+MGVQSEIDNEGFNNAVLR
Sbjct: 372 TVNWSASIQTPSHVTREWSDEYELELFGSNLYKEALEVVCKRMGVQSEIDNEGFNNAVLR 431

Query: 421 KGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGVILPGCEA 480
           KGCEELG   +NIPRNS SDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNG ILPGCEA
Sbjct: 432 KGCEELGCKAINIPRNSASDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA 491

Query: 481 IDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVTVVACGALSTPPLLRKSGLRN 540
           I I QE KDG +RNRAKGVAFEFGCGSGAKQM LVESKVT+VACGAL TP LL++SGLRN
Sbjct: 492 IRITQE-KDGRKRNRAKGVAFEFGCGSGAKQMCLVESKVTIVACGALCTPQLLKRSGLRN 551

Query: 541 PNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAMCNIGSEPDQEPGYGGAV 600
           PNIGKNLHLHPVTMAWG+FPAETD+SWPE+DKKSYEGGIMTAMCNIGS PDQEPGYGGAV
Sbjct: 552 PNIGKNLHLHPVTMAWGYFPAETDESWPEKDKKSYEGGIMTAMCNIGSSPDQEPGYGGAV 611

Query: 601 IQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDKGSGTIKSPSSFSYNMDD 660
           IQTPALHPGLFSILMPW+SGTNIKQRMCKFSRTAHVFALARDKGSGT++S SS SY MDD
Sbjct: 612 IQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFALARDKGSGTVESSSSLSYIMDD 671

Query: 661 VDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYEKFVKEESSRPLKDL 720
            DE+NLQKGLEKALKIL AAGAEEIGTHHNKGRSINVKKVSYREYE+FVKEESSRPLKDL
Sbjct: 672 GDEKNLQKGLEKALKILGAAGAEEIGTHHNKGRSINVKKVSYREYERFVKEESSRPLKDL 731

Query: 721 MTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA 780
           +TPICSAHQMGSCRMGI AKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA
Sbjct: 732 LTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA 791

Query: 781 YCTAQSVLETLKRRR 796
           YCTA+SVLETLKR+R
Sbjct: 792 YCTAKSVLETLKRKR 801

BLAST of Lcy12g013550 vs. ExPASy TrEMBL
Match: A0A6J1IPF0 (Long-chain-alcohol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111477017 PE=3 SV=1)

HSP 1 Score: 1370.9 bits (3547), Expect = 0.0e+00
Identity = 692/795 (87.04%), Postives = 732/795 (92.08%), Query Frame = 0

Query: 1   MGYFDATAAASCGGDGEAKRQLDHCVIDLHGGADRLHDGAPAGISQHANSNNSSLSAAEM 60
           MGYF   AA+S GGDGE+K QL  CVI+L+G AD LHDG PAGISQHANS N SLSAAEM
Sbjct: 12  MGYFG--AASSYGGDGESKPQLHQCVIELNGCADSLHDGGPAGISQHANS-NYSLSAAEM 71

Query: 61  ESLAALCDTFLPSVAAADRSAAVFFETSASTADTPERVGEMIKERVRHPKKWLARLSLWM 120
           ESLAALC+TFLPSVAA D SA+ FFETSAS A T ERVG MIKERVRHPKKWL R+SLWM
Sbjct: 72  ESLAALCETFLPSVAATDGSASEFFETSASAAGTSERVGGMIKERVRHPKKWLMRMSLWM 131

Query: 121 LSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFLLRMFFRTVKIFC 180
           LSTWYGTYI+CGN SLSN FPYFQRFSKVSQ++REQ+VVSWSLSYF LLRMFFRT+KI  
Sbjct: 132 LSTWYGTYILCGNGSLSNHFPYFQRFSKVSQKKREQVVVSWSLSYFLLLRMFFRTIKILS 191

Query: 181 LLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKENGRSREVEEDEEELYGP 240
            LAFFTQVD++MNNISW AIDYPG+DPQFKAK RQSE  NLKE  RSRE+EE+EEELYGP
Sbjct: 192 HLAFFTQVDEEMNNISWKAIDYPGSDPQFKAKARQSEEWNLKEKVRSREIEEEEEELYGP 251

Query: 241 LFKGIVNLEHQQVAAAEALRKFGFSVTLLSPRTKASSPSMIIKCDAVVVGSGSGGGVVAG 300
           LFKGIVNLEH QV A  ALRKFGF VTLLSP TK+ SPSMIIKCDAVVVGSGSGGGVV G
Sbjct: 252 LFKGIVNLEH-QVVAVGALRKFGFPVTLLSPSTKSPSPSMIIKCDAVVVGSGSGGGVVGG 311

Query: 301 VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDNMNVLVLAGSTVGGGS 360
           VLA+AGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGLVATDNMNVL+LAGSTVGGGS
Sbjct: 312 VLASAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNVLILAGSTVGGGS 371

Query: 361 TVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGVQSEIDNEGFNNAVLR 420
           TVNWSASIQTPSHVTREWSDEYELELF S+LYKEA+EVVCK+MGVQSEIDNEGFNNA+LR
Sbjct: 372 TVNWSASIQTPSHVTREWSDEYELELFRSNLYKEALEVVCKRMGVQSEIDNEGFNNAILR 431

Query: 421 KGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGVILPGCEA 480
           KGCEELG   +NIPRNS SDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNG ILPGCEA
Sbjct: 432 KGCEELGCKAINIPRNSASDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA 491

Query: 481 IDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVTVVACGALSTPPLLRKSGLRN 540
           I I QE KDG ERNRAKGVAFEF CGS AKQM LVESKVT+VACGAL TPPLL++SGLRN
Sbjct: 492 IRITQE-KDGRERNRAKGVAFEFECGSEAKQMCLVESKVTIVACGALCTPPLLKRSGLRN 551

Query: 541 PNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAMCNIGSEPDQEPGYGGAV 600
           PNIGKNLHLHPV MAWG+FPAETD+SWPE+DKKSYEGGIMTAMCNIGS PDQEPGYGGAV
Sbjct: 552 PNIGKNLHLHPVAMAWGYFPAETDESWPEKDKKSYEGGIMTAMCNIGSSPDQEPGYGGAV 611

Query: 601 IQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDKGSGTIKSPSSFSYNMDD 660
           IQTPALHPGLFSILMPW+SGTNIKQRMCKFSRTAHVFALARDKGSGT++S SS SY MDD
Sbjct: 612 IQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFALARDKGSGTVESSSSLSYIMDD 671

Query: 661 VDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYEKFVKEESSRPLKDL 720
            DE+NLQKGLEKALKIL AAGAEEIGTHHNKGRSINVKKVSYREYE+FVKEESSRPLKDL
Sbjct: 672 GDEKNLQKGLEKALKILGAAGAEEIGTHHNKGRSINVKKVSYREYERFVKEESSRPLKDL 731

Query: 721 MTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA 780
           +TPICSAHQMGSCRMGI AKDSVVNQRGETWEVEGLF+ADSSVFPTALGVNPMVTVQAIA
Sbjct: 732 LTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFVADSSVFPTALGVNPMVTVQAIA 791

Query: 781 YCTAQSVLETLKRRR 796
           YCTAQSVLETLKR+R
Sbjct: 792 YCTAQSVLETLKRKR 801

BLAST of Lcy12g013550 vs. ExPASy TrEMBL
Match: A0A6J1FQT3 (Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111446403 PE=3 SV=1)

HSP 1 Score: 1366.3 bits (3535), Expect = 0.0e+00
Identity = 699/811 (86.19%), Postives = 738/811 (91.00%), Query Frame = 0

Query: 1   MGYFDATAAA--------SC----GGDGEAKRQLDHCVIDLHGGADRLHDGAPAGISQHA 60
           MGYF A AAA        SC    GGD EAKR+LDH VI+       LHDGAPA I +HA
Sbjct: 32  MGYFGAAAAAEEVEVEVESCCGGGGGDDEAKRKLDHYVIE-------LHDGAPAEILEHA 91

Query: 61  NSNNS-SLSAAEMESLAALCDTFLPSVAAADRSAAVFFETSASTADTPERVGEMIKERVR 120
           NSNN+ SLSAAEMESLAALCDTFLPSV  AD SAA+FFETSA+ A TPE+VG+MIKERV+
Sbjct: 92  NSNNNCSLSAAEMESLAALCDTFLPSV-TADGSAAMFFETSAAAAGTPEKVGKMIKERVK 151

Query: 121 HPKKWLARLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFF 180
           HPKKWL R+SLWMLSTWYGTYI+CGN+SLS+ FPYFQ F K+SQ +REQIVVSWSLSYFF
Sbjct: 152 HPKKWLVRVSLWMLSTWYGTYILCGNRSLSHNFPYFQTFPKLSQTKREQIVVSWSLSYFF 211

Query: 181 LLRMFFRTVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKENGRS 240
           LLRM+FRT+KIFCLL FFTQVDDK NNISW AIDYPG DPQFKAKT+Q EA        S
Sbjct: 212 LLRMWFRTIKIFCLLTFFTQVDDKTNNISWQAIDYPGADPQFKAKTKQLEA--------S 271

Query: 241 REVEEDEEELYGPLFKGIVNLEHQQV---AAAEALRKFGFSVTLLSPRTKASSPSMIIKC 300
           REVEEDEEELYGPLFKGIV+L++QQV   AAAEALRKFGF VTLLSP  KASSPSMIIKC
Sbjct: 272 REVEEDEEELYGPLFKGIVHLDNQQVAAAAAAEALRKFGFPVTLLSPMIKASSPSMIIKC 331

Query: 301 DAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATD 360
           DAVVVGSGSGGGVVAGVLANAGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGLVATD
Sbjct: 332 DAVVVGSGSGGGVVAGVLANAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATD 391

Query: 361 NMNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMG 420
           NMNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSD+YELELFSS+LYKEAMEVVCKKMG
Sbjct: 392 NMNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDKYELELFSSNLYKEAMEVVCKKMG 451

Query: 421 VQSEIDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLV 480
           VQSEIDNEGFNNAVLRKGCEELG H +NIPRNS SDHYCGWCCLGCK+GRKKGTAETWLV
Sbjct: 452 VQSEIDNEGFNNAVLRKGCEELGYHAINIPRNSTSDHYCGWCCLGCKDGRKKGTAETWLV 511

Query: 481 DLVRSGNGVILPGCEAIDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVTVVAC 540
           DLVRSGNGVILPGCEAIDIR+EKKDGSERNRAKG+AFEFGCGSG KQ+ LVESKVT+VAC
Sbjct: 512 DLVRSGNGVILPGCEAIDIRKEKKDGSERNRAKGIAFEFGCGSGVKQVCLVESKVTIVAC 571

Query: 541 GALSTPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAMC 600
           GALSTPPLL +SGLRNPNIGKNLHLHPVTMAWGHFPAETDK WPEE KKSYEGGIMTAMC
Sbjct: 572 GALSTPPLLLRSGLRNPNIGKNLHLHPVTMAWGHFPAETDKPWPEEHKKSYEGGIMTAMC 631

Query: 601 NIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDKG 660
           NI SEPDQEPG GGAVIQTPALHPGLFSILMPW+SGTNIKQRM KFSRTAHVF LARDKG
Sbjct: 632 NIRSEPDQEPGSGGAVIQTPALHPGLFSILMPWLSGTNIKQRMRKFSRTAHVFVLARDKG 691

Query: 661 SGTIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYRE 720
           SGT+KSP+  SYNM++VDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYRE
Sbjct: 692 SGTVKSPNCISYNMEEVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYRE 751

Query: 721 YEKFVKEESSRPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIADSSVF 780
           YEKFVKEESSR LKDL TPICSAHQMGSCRMGI A+DSVV+QRGETWEVEGLFIADSSVF
Sbjct: 752 YEKFVKEESSRALKDLKTPICSAHQMGSCRMGIGARDSVVDQRGETWEVEGLFIADSSVF 811

Query: 781 PTALGVNPMVTVQAIAYCTAQSVLETLKRRR 796
           PTALGVNPMVTVQAIAYCTAQSVLETLKR+R
Sbjct: 812 PTALGVNPMVTVQAIAYCTAQSVLETLKRKR 826

BLAST of Lcy12g013550 vs. ExPASy TrEMBL
Match: A0A1S3BAP1 (Long-chain-alcohol oxidase OS=Cucumis melo OX=3656 GN=LOC103487628 PE=3 SV=1)

HSP 1 Score: 1349.7 bits (3492), Expect = 0.0e+00
Identity = 687/815 (84.29%), Postives = 727/815 (89.20%), Query Frame = 0

Query: 1   MGYFDATAAASCGGDGEAKRQ-LDHCVIDLHGGADRLHDGAPAGISQHANSNN------S 60
           MGYF A A    G DGE   Q   HCVI+LHGGAD  HDGA   ISQHANSNN       
Sbjct: 12  MGYFCAAANGGGGDDGETNHQPHHHCVIELHGGADSFHDGASVEISQHANSNNDYYSSAG 71

Query: 61  SLSAAEMESLAALCDTFLPSVA---AADRSAAVFFETSASTADTPERVGEMIKERVRHPK 120
            LSAAEMESLAALCDTFLPSVA   AADRS AVFFETSAS   TPERVGEMI ERVRHPK
Sbjct: 72  HLSAAEMESLAALCDTFLPSVAPATAADRSTAVFFETSASAVGTPERVGEMIMERVRHPK 131

Query: 121 KWLARLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFLLR 180
           KWL R+SLWMLSTWYGTYI+CGN+SLSN FPYF+RFSKVSQ++REQI +SWSLSYFFLLR
Sbjct: 132 KWLVRVSLWMLSTWYGTYILCGNESLSNHFPYFKRFSKVSQKKREQIALSWSLSYFFLLR 191

Query: 181 MFFRTVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKE-NGRSRE 240
           MFFRT+KI CLLAFFTQVD++MNNISW AIDYPG+DPQFK        SNLKE + +SRE
Sbjct: 192 MFFRTIKILCLLAFFTQVDEEMNNISWKAIDYPGSDPQFK------PPSNLKEKDSKSRE 251

Query: 241 V-------EEDEEELYGPLFKGIVNLEHQQ--VAAAEALRKFGFSVTLLSPRTKASSPSM 300
           V       EE+ EELYGPLFKGIVNL+HQQ    A  ALRKFGF VTLLSP TK  +PSM
Sbjct: 252 VDEEGDEEEEEAEELYGPLFKGIVNLQHQQQIQVAVGALRKFGFPVTLLSPTTKPPNPSM 311

Query: 301 IIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGL 360
           IIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEG AMEEMY SSGL
Sbjct: 312 IIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEMYQSSGL 371

Query: 361 VATDNMNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVC 420
           VATDNMNVL+LAGSTVGGGSTVNWSASI+TPSHVT+EWSDEYELELF S+LYKEA++VVC
Sbjct: 372 VATDNMNVLILAGSTVGGGSTVNWSASIKTPSHVTKEWSDEYELELFRSNLYKEAIDVVC 431

Query: 421 KKMGVQSEIDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAE 480
           KKMGVQS+IDNEGFNNAVLRKGCEELG H +NIPRNS SDHYCGWCCLGC++GRKKGTAE
Sbjct: 432 KKMGVQSKIDNEGFNNAVLRKGCEELGGHAINIPRNSTSDHYCGWCCLGCRDGRKKGTAE 491

Query: 481 TWLVDLVRSGNGVILPGCEAIDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVT 540
           TWLVDLVRSGNGVILPGCEAI IRQEKK G ER  AKGVAFEF CGS AKQM LVESKVT
Sbjct: 492 TWLVDLVRSGNGVILPGCEAIKIRQEKKQGRERKMAKGVAFEFWCGSAAKQMCLVESKVT 551

Query: 541 VVACGALSTPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIM 600
           +VACGAL+TPPLL++SGLRNPNIGKNLHLHPVTMAWG+FPAETDKSWPE+DKKSYEGGIM
Sbjct: 552 IVACGALNTPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETDKSWPEKDKKSYEGGIM 611

Query: 601 TAMCNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALA 660
           TAMCNIG EP+QEPGYGGAVIQTPALHPGLFSILMPW+SGTNIKQRMCKFSRTAHVF LA
Sbjct: 612 TAMCNIGPEPNQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFVLA 671

Query: 661 RDKGSGTIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKV 720
           RDKGSGT+KSPSS SY MD+VDE+NLQKGLEKALKILAAAGA EIGTHHNKGRSINVKKV
Sbjct: 672 RDKGSGTVKSPSSLSYKMDNVDEQNLQKGLEKALKILAAAGAVEIGTHHNKGRSINVKKV 731

Query: 721 SYREYEKFVKEESSRPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIAD 780
           SYREYEKFVKEESSRPLKDL+TPICSAHQMGSCRMG  AKDSVVNQRGETWEVEGLFIAD
Sbjct: 732 SYREYEKFVKEESSRPLKDLLTPICSAHQMGSCRMGSGAKDSVVNQRGETWEVEGLFIAD 791

Query: 781 SSVFPTALGVNPMVTVQAIAYCTAQSVLETLKRRR 796
           SSVFPTALGVNPMVTVQAIAYCTAQSVLETLKR+R
Sbjct: 792 SSVFPTALGVNPMVTVQAIAYCTAQSVLETLKRKR 820

BLAST of Lcy12g013550 vs. ExPASy TrEMBL
Match: A0A6J1CNU3 (Long-chain-alcohol oxidase OS=Momordica charantia OX=3673 GN=LOC111012719 PE=3 SV=1)

HSP 1 Score: 1324.7 bits (3427), Expect = 0.0e+00
Identity = 662/809 (81.83%), Postives = 718/809 (88.75%), Query Frame = 0

Query: 1   MGYFDATAAASCGGDGEAKRQLDHCVIDLHGGADRLHDGAPAGISQHAN-SNNSSLSAAE 60
           MGY  ATAAAS GGDG+ ++   HC+++LHGGAD + +G+P    QHA  S+N+SL+AAE
Sbjct: 1   MGYCGATAAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAE 60

Query: 61  MESLAALCDTFLPSVAAAD-----------RSAAVFFETSASTADTPERVGEMIKERVRH 120
           MESLAALCDTFLPSVAAA             + A FF+TSAS A TPERVG MI ERVRH
Sbjct: 61  MESLAALCDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRH 120

Query: 121 PKKWLARLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFL 180
           PKKW+ RLSLWMLSTWYGTYI+CGN SLSN FPYFQ FSK+S  +RE+I +SWSLS+F L
Sbjct: 121 PKKWMVRLSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLL 180

Query: 181 LRMFFRTVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKENGRSR 240
            RMFFRT+KI  LLAFF+QVD+KMNNISW AIDYPG DPQFK +TRQSEASNL ENGRSR
Sbjct: 181 HRMFFRTIKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSR 240

Query: 241 EVEEDEEELYGPLFKGIVNLEHQQVAAAEALRKFGFSVTLLSPRTKASSPSMIIKCDAVV 300
                EEELYGPLF+GIVN++ ++  AAEALRKFGF V  L PRTK +SPSM+IKCDAVV
Sbjct: 241 -----EEELYGPLFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVV 300

Query: 301 VGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDNMNV 360
           VGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGL+ATDNMNV
Sbjct: 301 VGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNV 360

Query: 361 LVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGVQSE 420
           LVLAGSTVGGGSTVNWSAS QTP+HVTREWSD YEL+LFSS+LYKEAMEVVCKKMGVQSE
Sbjct: 361 LVLAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSE 420

Query: 421 IDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVDLVR 480
           I++EGFNNAVLRKGCEELG H +NIPRNSP+DHYCGWCCLGCKNGRKKGTAETWLVDLVR
Sbjct: 421 IESEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVR 480

Query: 481 SGNGVILPGCEAIDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVTVVACGALS 540
           SGNG ILPGCEA+ I  EKKDGSER RAKG+ FEFGC SGAKQM LVESKVT+VACGALS
Sbjct: 481 SGNGAILPGCEAVRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALS 540

Query: 541 TPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAET--DKSWPEEDKKSYEGGIMTAMCNI 600
           TPPLL++SGLRNPNIGK+LHLHPVTMAWGHFPAET  DK+WPEE KKSYEGGIMTAMCNI
Sbjct: 541 TPPLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNI 600

Query: 601 GSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDKGSG 660
           G+E D EPGYGGAVIQTPALHPGLFSILMPW+SGTN K RMCKFSRTAHVFALARDKGSG
Sbjct: 601 GTEADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSG 660

Query: 661 TIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYE 720
           T++S SS SY MD  DEENLQKG+EKAL+ILAAAGAEEIGTHHNKGRSI VKKVSYREYE
Sbjct: 661 TVRSSSSISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYE 720

Query: 721 KFVKEESSRPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIADSSVFPT 780
           KFVKEESSRPL DL TPICSAHQMGSCRMGI  KDSVVNQRGETWE+EGLFIADSSVFPT
Sbjct: 721 KFVKEESSRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPT 780

Query: 781 ALGVNPMVTVQAIAYCTAQSVLETLKRRR 796
           ALGVNPMVTVQAIAYCTAQSVL+TLKR+R
Sbjct: 781 ALGVNPMVTVQAIAYCTAQSVLDTLKRKR 804

BLAST of Lcy12g013550 vs. NCBI nr
Match: XP_038896170.1 (long-chain-alcohol oxidase FAO4A [Benincasa hispida])

HSP 1 Score: 1389.0 bits (3594), Expect = 0.0e+00
Identity = 700/809 (86.53%), Postives = 742/809 (91.72%), Query Frame = 0

Query: 1   MGYFDATAAASCGG----DGEAKRQLDHCVIDLHGGADRLHDGAPAGISQHANSN--NSS 60
           MGYFD   AAS GG    DG++ +Q   CVI+LHGG D  HDGA  G SQHANS+  +S 
Sbjct: 12  MGYFD--TAASGGGSDIDDGQSNQQCQQCVIELHGGTDSFHDGASVGFSQHANSDDLSSH 71

Query: 61  LSAAEMESLAALCDTFLPSV---AAADRSAAVFFETSASTADTPERVGEMIKERVRHPKK 120
           LSAAEMESLAALCDTFLPSV   AAADRS  +FFETSAS A TPERVGEMIKERVRHPKK
Sbjct: 72  LSAAEMESLAALCDTFLPSVAATAAADRSVTLFFETSASAAGTPERVGEMIKERVRHPKK 131

Query: 121 WLARLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFLLRM 180
           WL R++LWMLSTWYGTYI+CGN+SLSN FPYF+RFSKVSQ++REQI +SWSLSYFFLLRM
Sbjct: 132 WLVRMTLWMLSTWYGTYILCGNESLSNHFPYFERFSKVSQKKREQIAISWSLSYFFLLRM 191

Query: 181 FFRTVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKENG-RSREV 240
           FFRT+KI CLLAFFTQVD++ NNISW AIDYPG+DPQFKAKT+ SEASNLKE G +SRE+
Sbjct: 192 FFRTIKILCLLAFFTQVDEERNNISWKAIDYPGSDPQFKAKTKHSEASNLKEKGSKSREI 251

Query: 241 --EEDEEELYGPLFKGIVNLEHQQ--VAAAEALRKFGFSVTLLSPRTKASSPSMIIKCDA 300
             E+DEEELYGPLFKGIVNL+HQQ    A  ALRKFGF VTLLS  TK  +PSMIIKCDA
Sbjct: 252 DQEDDEEELYGPLFKGIVNLQHQQQVQVAVGALRKFGFPVTLLSLSTKPPNPSMIIKCDA 311

Query: 301 VVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDNM 360
           VVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEG AMEEMY +SGLVATDNM
Sbjct: 312 VVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEMYQASGLVATDNM 371

Query: 361 NVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGVQ 420
           NVL+LAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELF S+LYKEAMEVVCKKMGVQ
Sbjct: 372 NVLILAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKEAMEVVCKKMGVQ 431

Query: 421 SEIDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVDL 480
           SEIDNEGFNNAVLRKGCEELG H MNIPRNS SDHYCGWCCLGCKNGRKKGTAETWLVDL
Sbjct: 432 SEIDNEGFNNAVLRKGCEELGCHAMNIPRNSTSDHYCGWCCLGCKNGRKKGTAETWLVDL 491

Query: 481 VRSGNGVILPGCEAIDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVTVVACGA 540
           VRSGNG ILPGCEAI IRQEK+DGS+ NRAKG+ FEFGCGS AKQM LVESKVT+VACGA
Sbjct: 492 VRSGNGAILPGCEAIKIRQEKRDGSKCNRAKGIVFEFGCGSAAKQMCLVESKVTIVACGA 551

Query: 541 LSTPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAMCNI 600
           L+TPPLL++SGLRNPNIGKNLHLHPVTMAWG+FPAETDKSWPE+DKKSYEGGIMTAMCNI
Sbjct: 552 LNTPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETDKSWPEKDKKSYEGGIMTAMCNI 611

Query: 601 GSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDKGSG 660
           GSEPDQEPGYGGAVIQTPALHPGLFSILMPW+SGTNIK RMCKFSRTAHVFALARDKGSG
Sbjct: 612 GSEPDQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKHRMCKFSRTAHVFALARDKGSG 671

Query: 661 TIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYE 720
           T+KS SS SY MDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYE
Sbjct: 672 TVKSSSSLSYKMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYE 731

Query: 721 KFVKEESSRPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIADSSVFPT 780
           KFVKEESSRPLKDL+TPICSAHQMGSCRMGI AKDSVVNQRGETWEVEGLFIADSSVFPT
Sbjct: 732 KFVKEESSRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPT 791

Query: 781 ALGVNPMVTVQAIAYCTAQSVLETLKRRR 796
           ALGVNPMVTVQAIAYCTAQS+LETLKR+R
Sbjct: 792 ALGVNPMVTVQAIAYCTAQSILETLKRKR 818

BLAST of Lcy12g013550 vs. NCBI nr
Match: XP_022937010.1 (long-chain-alcohol oxidase FAO4A-like [Cucurbita moschata])

HSP 1 Score: 1387.9 bits (3591), Expect = 0.0e+00
Identity = 699/795 (87.92%), Postives = 737/795 (92.70%), Query Frame = 0

Query: 1   MGYFDATAAASCGGDGEAKRQLDHCVIDLHGGADRLHDGAPAGISQHANSNNSSLSAAEM 60
           MGYF   AAASCGGDGE+K QL  CVI+L+GGAD LHDG PAGISQHAN  N SLSAAEM
Sbjct: 12  MGYFG--AAASCGGDGESKPQLHQCVIELNGGADSLHDGGPAGISQHANF-NYSLSAAEM 71

Query: 61  ESLAALCDTFLPSVAAADRSAAVFFETSASTADTPERVGEMIKERVRHPKKWLARLSLWM 120
           ESLAALCDTFLPSVAA D SA+ FFETSAS A TPERVG MIKERVRHPKKWL R+SLWM
Sbjct: 72  ESLAALCDTFLPSVAATDGSASEFFETSASAAGTPERVGGMIKERVRHPKKWLMRVSLWM 131

Query: 121 LSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFLLRMFFRTVKIFC 180
           LSTWYGTYI+CGN SLSN FPYFQRFSKVSQ++REQ+VVSWSLSYF LLRMFFRT+KI C
Sbjct: 132 LSTWYGTYILCGNGSLSNHFPYFQRFSKVSQKKREQVVVSWSLSYFLLLRMFFRTIKILC 191

Query: 181 LLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKENGRSREVEEDEEELYGP 240
            LAFFTQVD++MNNISW AIDYPG+DPQFKAK RQSE  NLKE  RSRE+ E+EEELYGP
Sbjct: 192 HLAFFTQVDEEMNNISWKAIDYPGSDPQFKAKARQSEEWNLKEKVRSREIVEEEEELYGP 251

Query: 241 LFKGIVNLEHQQVAAAEALRKFGFSVTLLSPRTKASSPSMIIKCDAVVVGSGSGGGVVAG 300
           LFKGIVNLEHQQVA   ALRKFGF VTLLSP  K+ SPSMIIKCDAVVVGSGSGGGVVAG
Sbjct: 252 LFKGIVNLEHQQVAVG-ALRKFGFPVTLLSPSIKSPSPSMIIKCDAVVVGSGSGGGVVAG 311

Query: 301 VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDNMNVLVLAGSTVGGGS 360
           VLA+AGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGLVATDNMNVL+LAGSTVGGGS
Sbjct: 312 VLASAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNVLILAGSTVGGGS 371

Query: 361 TVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGVQSEIDNEGFNNAVLR 420
           TVNWSASIQTPSHVTREWSDEYELELF S+LYKEA+EVVCK+MGVQSEIDNEGFNNAVLR
Sbjct: 372 TVNWSASIQTPSHVTREWSDEYELELFGSNLYKEALEVVCKRMGVQSEIDNEGFNNAVLR 431

Query: 421 KGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGVILPGCEA 480
           KGCEELG   +NIPRNS SDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNG ILPGCEA
Sbjct: 432 KGCEELGCKAINIPRNSASDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA 491

Query: 481 IDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVTVVACGALSTPPLLRKSGLRN 540
           I I QE KDG +RNRAKGVAFEFGCGSGAKQM LVESKVT+VACGAL TP LL++SGLRN
Sbjct: 492 IRITQE-KDGRKRNRAKGVAFEFGCGSGAKQMCLVESKVTIVACGALCTPQLLKRSGLRN 551

Query: 541 PNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAMCNIGSEPDQEPGYGGAV 600
           PNIGKNLHLHPVTMAWG+FPAETD+SWPE+DKKSYEGGIMTAMCNIGS PDQEPGYGGAV
Sbjct: 552 PNIGKNLHLHPVTMAWGYFPAETDESWPEKDKKSYEGGIMTAMCNIGSSPDQEPGYGGAV 611

Query: 601 IQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDKGSGTIKSPSSFSYNMDD 660
           IQTPALHPGLFSILMPW+SGTNIKQRMCKFSRTAHVFALARDKGSGT++S SS SY MDD
Sbjct: 612 IQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFALARDKGSGTVESSSSLSYIMDD 671

Query: 661 VDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYEKFVKEESSRPLKDL 720
            DE+NLQKGLEKALKIL AAGAEEIGTHHNKGRSINVKKVSYREYE+FVKEESSRPLKDL
Sbjct: 672 GDEKNLQKGLEKALKILGAAGAEEIGTHHNKGRSINVKKVSYREYERFVKEESSRPLKDL 731

Query: 721 MTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA 780
           +TPICSAHQMGSCRMGI AKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA
Sbjct: 732 LTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA 791

Query: 781 YCTAQSVLETLKRRR 796
           YCTA+SVLETLKR+R
Sbjct: 792 YCTAKSVLETLKRKR 801

BLAST of Lcy12g013550 vs. NCBI nr
Match: XP_023535226.1 (long-chain-alcohol oxidase FAO4A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1385.2 bits (3584), Expect = 0.0e+00
Identity = 702/795 (88.30%), Postives = 737/795 (92.70%), Query Frame = 0

Query: 1   MGYFDATAAASCGGDGEAKRQLDHCVIDLHGGADRLHDGAPAGISQHANSNNSSLSAAEM 60
           MGYF   AAAS GG+GE+K QL  CVIDLHGGAD  +DG PAGISQHANS N SLSAAEM
Sbjct: 12  MGYFG--AAASYGGNGESKPQLHQCVIDLHGGAD--NDGGPAGISQHANS-NYSLSAAEM 71

Query: 61  ESLAALCDTFLPSVAAADRSAAVFFETSASTADTPERVGEMIKERVRHPKKWLARLSLWM 120
           ESLAALCDTFLPSVAA D SA+ +FETSAS A TPERVG MIKERVRHPKKWL R+SLWM
Sbjct: 72  ESLAALCDTFLPSVAATDGSASEYFETSASAAGTPERVGGMIKERVRHPKKWLMRVSLWM 131

Query: 121 LSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFLLRMFFRTVKIFC 180
           LSTWYGTYI+CGN SLSN FPYFQRFSKVSQR REQ+VVSWSLSYF LLRMF+RTVKI C
Sbjct: 132 LSTWYGTYILCGNGSLSNHFPYFQRFSKVSQREREQVVVSWSLSYFLLLRMFYRTVKILC 191

Query: 181 LLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKENGRSREVEEDEEELYGP 240
            LAFFTQVD++MNNISW AIDYPG+DPQFKAK RQSE  NLKE  RSRE+EE+EEELYGP
Sbjct: 192 HLAFFTQVDEEMNNISWKAIDYPGSDPQFKAKARQSEEWNLKEKVRSREIEEEEEELYGP 251

Query: 241 LFKGIVNLEHQQVAAAEALRKFGFSVTLLSPRTKASSPSMIIKCDAVVVGSGSGGGVVAG 300
           LFKGIVNLEHQQVA   ALRKFGF VTLLSP TK+ SPSMIIKCDAVVVGSGSGGGVVAG
Sbjct: 252 LFKGIVNLEHQQVAVG-ALRKFGFPVTLLSPSTKSPSPSMIIKCDAVVVGSGSGGGVVAG 311

Query: 301 VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDNMNVLVLAGSTVGGGS 360
           VLA+AGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGLVATDNMNVL+LAGSTVGGGS
Sbjct: 312 VLASAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNVLILAGSTVGGGS 371

Query: 361 TVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGVQSEIDNEGFNNAVLR 420
           TVNWSASIQTPSHVTREWSDEYELELF S+LYKEA+EVVCK+MGVQSEI+NEGFNNAVLR
Sbjct: 372 TVNWSASIQTPSHVTREWSDEYELELFRSNLYKEALEVVCKRMGVQSEIENEGFNNAVLR 431

Query: 421 KGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGVILPGCEA 480
           KGCEELG   MNIPRNS SDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNG ILPGCEA
Sbjct: 432 KGCEELGCKAMNIPRNSASDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA 491

Query: 481 IDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVTVVACGALSTPPLLRKSGLRN 540
           I I QE KDG ERNRAKGVAFEF CGSGAKQM LVESKVT+VACGAL TPPLL++SGLRN
Sbjct: 492 IRITQE-KDGRERNRAKGVAFEFECGSGAKQMCLVESKVTIVACGALCTPPLLKRSGLRN 551

Query: 541 PNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAMCNIGSEPDQEPGYGGAV 600
           PNIGKNLHLHPVTMAWG+FPAETD+SWPE+DKKSYEGGIMTAMCNIGS PDQEPGYGGAV
Sbjct: 552 PNIGKNLHLHPVTMAWGYFPAETDESWPEKDKKSYEGGIMTAMCNIGSSPDQEPGYGGAV 611

Query: 601 IQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDKGSGTIKSPSSFSYNMDD 660
           IQTPALHPGLFSILMPW+SGTNIKQRMCKFSRTAHVFALARDKGSGT++S SS SY MDD
Sbjct: 612 IQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFALARDKGSGTVESSSSLSYIMDD 671

Query: 661 VDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYEKFVKEESSRPLKDL 720
            DE+NLQKGLEKALKIL AAGAEEIGTHHNKGRSINVKKVSYREYE+FVKEESSRPLKDL
Sbjct: 672 GDEKNLQKGLEKALKILGAAGAEEIGTHHNKGRSINVKKVSYREYERFVKEESSRPLKDL 731

Query: 721 MTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA 780
           +TPICSAHQMGSCRMGI AKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA
Sbjct: 732 LTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA 791

Query: 781 YCTAQSVLETLKRRR 796
           YCTAQSVLETLKR+R
Sbjct: 792 YCTAQSVLETLKRKR 799

BLAST of Lcy12g013550 vs. NCBI nr
Match: KAG6591858.1 (Long-chain-alcohol oxidase FAO4A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1380.2 bits (3571), Expect = 0.0e+00
Identity = 694/795 (87.30%), Postives = 735/795 (92.45%), Query Frame = 0

Query: 1   MGYFDATAAASCGGDGEAKRQLDHCVIDLHGGADRLHDGAPAGISQHANSNNSSLSAAEM 60
           MGYF AT  AS GGDGE+K QL  CVI+L+GGAD  +DG PAGISQHANS N SLSAAEM
Sbjct: 12  MGYFGAT--ASYGGDGESKPQLHQCVIELNGGADSRYDGGPAGISQHANS-NYSLSAAEM 71

Query: 61  ESLAALCDTFLPSVAAADRSAAVFFETSASTADTPERVGEMIKERVRHPKKWLARLSLWM 120
           ESLAALCDTFLPSVAA D SA+ FFETSAS A TPERVG MIKERVRHPKKWL R+SLWM
Sbjct: 72  ESLAALCDTFLPSVAATDGSASEFFETSASAAGTPERVGGMIKERVRHPKKWLMRVSLWM 131

Query: 121 LSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFLLRMFFRTVKIFC 180
           LSTWYGTYI+CGN SLSN FPYFQRFSKVSQ++REQ+VVSWSLSYF LL+MFFRT+KI C
Sbjct: 132 LSTWYGTYILCGNGSLSNHFPYFQRFSKVSQKKREQVVVSWSLSYFLLLKMFFRTIKILC 191

Query: 181 LLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKENGRSREVEEDEEELYGP 240
            LAFFTQVD++MNNISW AIDYPG+DPQFKAK RQSE  NLKE  RSRE+EE+EEELYGP
Sbjct: 192 HLAFFTQVDEEMNNISWKAIDYPGSDPQFKAKARQSEEWNLKEKVRSREIEEEEEELYGP 251

Query: 241 LFKGIVNLEHQQVAAAEALRKFGFSVTLLSPRTKASSPSMIIKCDAVVVGSGSGGGVVAG 300
           LFKGIVNLEHQQVA   ALRKFGF VTLLSP  K+ SPSMIIKCDAVVVGSGSGGGVVAG
Sbjct: 252 LFKGIVNLEHQQVAVG-ALRKFGFPVTLLSPSIKSPSPSMIIKCDAVVVGSGSGGGVVAG 311

Query: 301 VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDNMNVLVLAGSTVGGGS 360
           VLA+AGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGLVATDNMNVL+LAGSTVGGGS
Sbjct: 312 VLASAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNVLILAGSTVGGGS 371

Query: 361 TVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGVQSEIDNEGFNNAVLR 420
           TVNWSASIQTPSHVTREWS EYELELF S+LYKEA+EVVCK+MGVQSE+DNEGFNNA+LR
Sbjct: 372 TVNWSASIQTPSHVTREWSGEYELELFGSNLYKEALEVVCKRMGVQSEMDNEGFNNAILR 431

Query: 421 KGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGVILPGCEA 480
           KGCEELG   MNIPRNS SDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNG ILPGCEA
Sbjct: 432 KGCEELGCKAMNIPRNSASDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA 491

Query: 481 IDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVTVVACGALSTPPLLRKSGLRN 540
           I I QE KDG +RNRAKGVAFEFGCGSGAKQM LVESKVT+VACGAL TPPLL++SGL N
Sbjct: 492 IRITQE-KDGRKRNRAKGVAFEFGCGSGAKQMCLVESKVTIVACGALCTPPLLKRSGLTN 551

Query: 541 PNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAMCNIGSEPDQEPGYGGAV 600
           PNIGKNLHLHPV MAWG+FPAETD+SWPE+DKKSYEGGIMTAMCNIGS PDQEPGYGGAV
Sbjct: 552 PNIGKNLHLHPVAMAWGYFPAETDESWPEKDKKSYEGGIMTAMCNIGSSPDQEPGYGGAV 611

Query: 601 IQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDKGSGTIKSPSSFSYNMDD 660
           IQTPALHPGLFSILMPW+SGTNIKQ+MCKFSRTAHVFALARDKGSGT++S SS SY MDD
Sbjct: 612 IQTPALHPGLFSILMPWVSGTNIKQKMCKFSRTAHVFALARDKGSGTVESSSSLSYIMDD 671

Query: 661 VDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYEKFVKEESSRPLKDL 720
            DE+NLQKGLEKALKIL AAGAEEIGTHHNKGRSINVKKVSYREYE+FVKEESSRPLKDL
Sbjct: 672 GDEKNLQKGLEKALKILGAAGAEEIGTHHNKGRSINVKKVSYREYERFVKEESSRPLKDL 731

Query: 721 MTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA 780
           +TPICSAHQMGSCRMGI AKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA
Sbjct: 732 LTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA 791

Query: 781 YCTAQSVLETLKRRR 796
           YCTAQSVLETLKR+R
Sbjct: 792 YCTAQSVLETLKRKR 801

BLAST of Lcy12g013550 vs. NCBI nr
Match: XP_023523874.1 (long-chain-alcohol oxidase FAO4A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1377.8 bits (3565), Expect = 0.0e+00
Identity = 703/809 (86.90%), Postives = 741/809 (91.59%), Query Frame = 0

Query: 1   MGYFDATAAA-------SC---GGDGEAKRQLDHCVIDLHGGADRLHDGAPAGISQHANS 60
           MGYF A AAA       SC   GGD EAKR+LDH VI+       LHDGAPA I +HANS
Sbjct: 1   MGYFGAAAAAAEEVEVESCGGGGGDDEAKRKLDHYVIE-------LHDGAPAEILEHANS 60

Query: 61  NNS-SLSAAEMESLAALCDTFLPSVA-AADRSAAVFFETSASTADTPERVGEMIKERVRH 120
           NN+ SLSAAEMESLAALCDTFLPSV   AD SAA+FFETSAS A TPE+VG+MIKERV+H
Sbjct: 61  NNNCSLSAAEMESLAALCDTFLPSVVNTADGSAAMFFETSASAAGTPEKVGKMIKERVKH 120

Query: 121 PKKWLARLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFL 180
           PKKWL R+SLWMLSTWYGTYI+CGN+SLS+ FPYFQ F K+SQ +REQIVVSWSLSYFFL
Sbjct: 121 PKKWLVRVSLWMLSTWYGTYILCGNRSLSHHFPYFQTFPKLSQTKREQIVVSWSLSYFFL 180

Query: 181 LRMFFRTVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKENGRSR 240
           LRM+FRT+KIFCLL FFTQVDDK NNISW AIDYPG DPQFKAKT+Q EASN KEN RSR
Sbjct: 181 LRMWFRTIKIFCLLTFFTQVDDKTNNISWQAIDYPGADPQFKAKTKQLEASNQKENDRSR 240

Query: 241 EVEEDEEELYGPLFKGIVNLEHQQ--VAAAEALRKFGFSVTLLSPRTKASSPSMIIKCDA 300
           EVEED+EELYGPLFKGIVNL++QQ   AAAEALRKFGF VTLLSP  KASSPSMIIKCDA
Sbjct: 241 EVEEDKEELYGPLFKGIVNLDNQQAAAAAAEALRKFGFPVTLLSPMIKASSPSMIIKCDA 300

Query: 301 VVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDNM 360
           VVVGSGSGGGVVAGVLANAGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGLVATDNM
Sbjct: 301 VVVGSGSGGGVVAGVLANAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNM 360

Query: 361 NVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGVQ 420
           NVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSS+LYKEAMEVVCKKMGVQ
Sbjct: 361 NVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSNLYKEAMEVVCKKMGVQ 420

Query: 421 SEIDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVDL 480
           SEI NEGFNNAVLRKGCEELG H +NIPRNS SDHYCGWCCLGCK+GRKKGTAETWLVDL
Sbjct: 421 SEIHNEGFNNAVLRKGCEELGYHAINIPRNSTSDHYCGWCCLGCKDGRKKGTAETWLVDL 480

Query: 481 VRSGNGVILPGCEAIDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVTVVACGA 540
           VRSGNGVILPGCEAI+IR+EK DGSERNRAKGVAFEFGCGSG KQ+ LVESKVT+VACGA
Sbjct: 481 VRSGNGVILPGCEAINIRKEKNDGSERNRAKGVAFEFGCGSGVKQVCLVESKVTIVACGA 540

Query: 541 LSTPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAMCNI 600
           LSTPPLL +SGLRNPNIGKNLHLHPVTMAWGHFPAETDK WPEE KKSYEGGIMTAMCNI
Sbjct: 541 LSTPPLLLRSGLRNPNIGKNLHLHPVTMAWGHFPAETDKPWPEEHKKSYEGGIMTAMCNI 600

Query: 601 GSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDKGSG 660
            SEPDQEPG GGAVIQTPALHPGLFSILMPW+SG+NIKQRM KFSRTAHVF LARDKGSG
Sbjct: 601 RSEPDQEPGSGGAVIQTPALHPGLFSILMPWLSGSNIKQRMRKFSRTAHVFVLARDKGSG 660

Query: 661 TIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYE 720
           T+KSP+  SYNM++VDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYE
Sbjct: 661 TVKSPNCISYNMEEVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYE 720

Query: 721 KFVKEESSRPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIADSSVFPT 780
           KFVKEESSR LKDL TPICSAHQMGSCRMGI A+DSVV+QRGETWEVEGLFIADSSVFPT
Sbjct: 721 KFVKEESSRALKDLKTPICSAHQMGSCRMGIGARDSVVDQRGETWEVEGLFIADSSVFPT 780

Query: 781 ALGVNPMVTVQAIAYCTAQSVLETLKRRR 796
           ALGVNPMVTVQAIAYCTAQSVLETLKR+R
Sbjct: 781 ALGVNPMVTVQAIAYCTAQSVLETLKRKR 802

BLAST of Lcy12g013550 vs. TAIR 10
Match: AT4G19380.1 (Long-chain fatty alcohol dehydrogenase family protein )

HSP 1 Score: 866.3 bits (2237), Expect = 1.9e-251
Identity = 438/744 (58.87%), Postives = 560/744 (75.27%), Query Frame = 0

Query: 60  MESLAALCDTFLPS-----VAAADRSAAVFFETSASTADTPERVGEMIKERVRHPKKWLA 119
           MESL A+CDTF+ S     V   D   A +F  SAS   TP+RV  ++ ER+ HPKKW+ 
Sbjct: 1   MESLVAICDTFISSIDDSGVGHVDDCVAGYFSASASQTGTPDRVARLMSERLHHPKKWIL 60

Query: 120 RLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFLLRMFFR 179
           R  LW+LSTW G+ ++CG +S + +FPYF+RF ++ ++RRE+I+++WS SYF LLRM FR
Sbjct: 61  RAGLWLLSTWIGSLVLCGWRSFTGEFPYFRRFCRLPEKRREEILLNWSSSYFSLLRMLFR 120

Query: 180 TVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKENGRSREVEEDE 239
           T+K+   L FFTQVD+K  N++W AI Y G  P             L E  + ++     
Sbjct: 121 TIKLISALVFFTQVDEKGRNLAWKAIGYNGPSPD-----HSDHEVELNEEKKKKK----P 180

Query: 240 EELYGPLFKGIVNLEHQQVAAAEALRKFGFSVTLLSPRTKASS---PSMIIKCDAVVVGS 299
           EE++GPL+ GIV+L+  + A  + L   GF+V+     T  SS   P M I+CDAVVVGS
Sbjct: 181 EEIFGPLYNGIVDLKSPREAVEKKLAGRGFAVSNQKRNTNGSSISDPVMKIQCDAVVVGS 240

Query: 300 GSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDNMNVLVL 359
           GSGGGV AGVLA AGYKVLV+E G+Y AR+ LSLLEGQAM++MYLS GL+AT + NV++L
Sbjct: 241 GSGGGVAAGVLAKAGYKVLVIESGNYYARSKLSLLEGQAMDDMYLSGGLLATSDTNVVIL 300

Query: 360 AGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGVQSEIDN 419
           AGSTVGGGST+NWSASI+TP HV +EW+++ +LE+F S LY+EAM+VVCK+MGVQ     
Sbjct: 301 AGSTVGGGSTINWSASIKTPEHVMKEWAEKSKLEMFGSDLYREAMDVVCKRMGVQCGFVE 360

Query: 420 EGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGN 479
           EGFNN VLRKGCE+LG  V NIPRN+PSDHYCG+CCLGCK G+K+GT+ETWLVDLV S N
Sbjct: 361 EGFNNEVLRKGCEKLGLPVKNIPRNAPSDHYCGFCCLGCKKGQKQGTSETWLVDLVESDN 420

Query: 480 GVILPGCEAIDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVTVVACGALSTPP 539
           G+ILPGC+A ++  + + G ++ +A GVAF FG     +++++VES+VT+VACGAL TP 
Sbjct: 421 GLILPGCQATEVMYDCEQG-KKKKATGVAFAFG-----EEIYVVESRVTIVACGALRTPH 480

Query: 540 LLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAMCNIGSEPD 599
           LL++SGL+N NIG+NL LHPV MAWG FP E DK WPE+ KKSYEGGIMTAM ++  E +
Sbjct: 481 LLKRSGLKNSNIGRNLCLHPVVMAWGWFP-EEDK-WPEKKKKSYEGGIMTAMSSVVIE-E 540

Query: 600 QEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDKGSGTIKSP 659
               YG  VIQTPALHPG+FS ++PW S  + K RM KFSRTAH+FAL RDKG+GTI S 
Sbjct: 541 THSSYGEMVIQTPALHPGMFSGIIPWTSSKDFKTRMLKFSRTAHIFALLRDKGTGTIDSK 600

Query: 660 SSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYEKFVKE 719
           +   YN++D DEE+L+ GLE+ LKILAAAGAEEIGTHH++GRS+NV+  S  E E+FV+E
Sbjct: 601 TYIDYNLNDEDEESLKNGLERVLKILAAAGAEEIGTHHSEGRSLNVRTASSLEIERFVRE 660

Query: 720 ESSRPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIADSSVFPTALGVN 779
           ESS+PLKDL   ICSAHQMGSCRMGIR ++S V   GETWEVE LF+AD+SVFPTALGVN
Sbjct: 661 ESSKPLKDLSGQICSAHQMGSCRMGIRPEESAVRPTGETWEVERLFVADTSVFPTALGVN 720

Query: 780 PMVTVQAIAYCTAQSVLETLKRRR 796
           PMVTVQ+IAYC   +V++ LK+++
Sbjct: 721 PMVTVQSIAYCIGLNVVDVLKKKK 726

BLAST of Lcy12g013550 vs. TAIR 10
Match: AT4G28570.1 (Long-chain fatty alcohol dehydrogenase family protein )

HSP 1 Score: 525.0 bits (1351), Expect = 1.0e-148
Identity = 305/755 (40.40%), Postives = 441/755 (58.41%), Query Frame = 0

Query: 52  NSSLSAAEMESLAALCDTFLPSVAAADRSAAVFFETSASTADT-PERVGEMIKERVRHPK 111
           N   S ++++SLA +C TFLP    +++ A   F  ++ST     + V EMI +  R   
Sbjct: 24  NHGFSPSQIQSLAVICQTFLPPETTSEQQAVNSFHVASSTQPPFTDEVAEMIVKNGRSEA 83

Query: 112 KWLARLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFLL- 171
             + R+ L +LS  +GT ++CG+  L   +P+  +FS++   +RE I+ +WS    FLL 
Sbjct: 84  VKVLRIILMILSFRFGTLLLCGSLCLDKSWPFVLKFSQLPLDKREAILRNWSRQSGFLLP 143

Query: 172 -RMFFRTVKIFCLLAFFTQVDDKMNNISWNAIDY--PGTDPQFKAKTRQSEASNLKENGR 231
            R+ F   K + L  FF+Q D+ + N +  AI Y   GT+                   R
Sbjct: 144 FRITFFLAKFYTLFYFFSQTDENLKNPALEAIGYCIDGTE-------------------R 203

Query: 232 SREVEEDEEELYGPLFKGIVNLEHQ-QVAAAEALRKFGFSVTLLSPRTKASSPSMIIKCD 291
           S   + + +E   PL KGI+   H+  V   ++L + G  V               I+CD
Sbjct: 204 SSNKKSEADEKRRPLEKGIIETMHESDVTITQSLTEKGVHVA-----RDDGDNVYRIRCD 263

Query: 292 AVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDN 351
           AVVVGSGSGGGV A  LA AG KVLVLEKG+Y    + S LE  +M E+Y   GL+ T +
Sbjct: 264 AVVVGSGSGGGVAAANLAKAGLKVLVLEKGNYFTAHDYSGLEVPSMLELYEKGGLLTTVD 323

Query: 352 MNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGV 411
              ++LAGS VGGG+ VNWSASI+TP HV +EWS+  +++ F S  Y+ AM+ V  ++GV
Sbjct: 324 GKFMLLAGSAVGGGTAVNWSASIRTPDHVLQEWSEGSKIKFFGSQEYQSAMDEVTIRIGV 383

Query: 412 QSEIDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVD 471
                  GF N VLRKGCE LG  V ++PRNSP DHYCG C  GC+ G K GT +TWLVD
Sbjct: 384 TERCVKHGFQNQVLRKGCERLGLQVESVPRNSPEDHYCGLCGYGCRAGAKNGTDQTWLVD 443

Query: 472 LVRSGNGVILPGCEAIDIRQEKKDGSERNRAKGVAFEFGCGSGAK--QMFLVESKVTVVA 531
            V +G  VIL G +A          S   R K     F    G K  + F++E++VTV +
Sbjct: 444 AVENG-AVILTGIKAERFVLVDNTSSSNERKKRCVGVFASSVGGKIGKKFIIEARVTVSS 503

Query: 532 CGALSTPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAM 591
            G+L TPPL+  SGL+NPNIG+NL LHPV M WG+FP E D    E   K YEGGI+T++
Sbjct: 504 AGSLLTPPLMLSSGLKNPNIGRNLKLHPVLMTWGYFP-EKDS---EFSGKMYEGGIITSV 563

Query: 592 CNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDK 651
            ++    D E G   A+++ P + P  ++ L PW+SG ++K+RM K+ RTAH+FAL RD 
Sbjct: 564 HHMN---DTESGC-KAILENPLIGPASYAGLSPWVSGPDLKERMIKYGRTAHLFALVRDL 623

Query: 652 GSGTIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYR 711
           GSG +   +  +Y     D ENL+ GL +AL++  AAGA E+GT+ + G+ +  + ++  
Sbjct: 624 GSGEVMMENEVTYRTTKKDRENLRAGLRQALRVSVAAGAVEVGTYRSDGQKMKCEAITKE 683

Query: 712 EYEKFVKEESS----RPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIA 771
             E+F+ E  +        +  T   SAHQMGSCRMG+ A++  +++ GE+WE EGLF+ 
Sbjct: 684 AMEEFLDEVDAVGGVGTKGEYWTTYFSAHQMGSCRMGVTAEEGALDENGESWEAEGLFVC 743

Query: 772 DSSVFPTALGVNPMVTVQAIAYCTAQSVLETLKRR 795
           D S+ P+A+GVNPM+T+Q+ AYC +  ++++L+ +
Sbjct: 744 DGSILPSAVGVNPMITIQSTAYCISSKIVDSLQNK 745

BLAST of Lcy12g013550 vs. TAIR 10
Match: AT3G23410.1 (fatty alcohol oxidase 3 )

HSP 1 Score: 522.7 bits (1345), Expect = 5.2e-148
Identity = 307/759 (40.45%), Postives = 447/759 (58.89%), Query Frame = 0

Query: 55  LSAAEMESLAALCDTFLPSVAAADRSAA-------------VFFETSASTADTPERVGEM 114
           ++ AEMESLA+ C+  LPSV       +              F+ TS S      +  E+
Sbjct: 15  ITVAEMESLASFCEAVLPSVQPPPEELSGEGDNHRNKEALRSFYSTSGSKTPVLRQSIEL 74

Query: 115 IKERVRHPKKWLARLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSW 174
           + +R         RL L++L+T  GT ++CG + L +++P+ ++FS++S  +RE+++   
Sbjct: 75  VTKRGTIEAYIATRLILFLLATRLGTLLICGTECLVSRWPFVEKFSELSLEKRERVLQKQ 134

Query: 175 SLSYFFL-LRMFFRTVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASN 234
             ++    +R  F  +K+  L  FF++V+    N +W AI Y   +P    + + SE  N
Sbjct: 135 FKNWILTPIRAAFVYIKVAFLFCFFSRVNPNGENPAWEAIGY-RVNPD---ENKPSETHN 194

Query: 235 LKENGRSREVEEDEEELYGPLFKGIV-NLEHQQVAAAEALRKFGFSVTLLSPRTKASSPS 294
            +                 PL KGIV  +E  +    E+L   G    L          +
Sbjct: 195 ER-----------------PLEKGIVETMEETEQTLLESLAHKGLEAVL-----DTEHDA 254

Query: 295 MIIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSG 354
           + IKCD VVVGSGSGGGV A VLA +G KV+VLEKGSY   +     EG  ++++Y + G
Sbjct: 255 IRIKCDVVVVGSGSGGGVAASVLAKSGLKVVVLEKGSYFTPSEHRPFEGPGLDKLYENGG 314

Query: 355 LVATDNMNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVV 414
           ++ + + + +VLAG+TVGGGS VNWSA I+TP  V +EWS++  + LF +  Y  AMEVV
Sbjct: 315 ILPSVDGSFMVLAGATVGGGSAVNWSACIKTPKSVLQEWSEDQNIPLFGTKEYLTAMEVV 374

Query: 415 CKKMGVQSEIDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTA 474
            K+MGV  + + E F N +LRKGCE LG +V N+PRNS   HYCG C  GC+ G KKG+ 
Sbjct: 375 WKRMGVTEKCELESFQNQILRKGCENLGFNVENVPRNSSESHYCGSCGYGCRQGDKKGSD 434

Query: 475 ETWLVDLVRSGNGVILPGCEAIDIRQEK---KDGSERNRAKGVAFEFGCGSGAKQMFLVE 534
            TWLVD V  G  VIL GC+A     EK     G ++ +  GV  +   G+ AK M  +E
Sbjct: 435 RTWLVDAVGHG-AVILTGCKAERFILEKNGSNKGGKQMKCLGVMAKSLNGNIAK-MLKIE 494

Query: 535 SKVTVVACGALSTPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYE 594
           +KVTV A GAL TPPL+  SGLRN NIGKNLHLHPV MAWG+FP + + S       SYE
Sbjct: 495 AKVTVSAGGALLTPPLMISSGLRNRNIGKNLHLHPVLMAWGYFP-DKESSNISFKGNSYE 554

Query: 595 GGIMTAMCNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHV 654
           GGI+T++  + SE  +      A+I+TP L PG FS+L PW SG ++K+RM ++SRTA +
Sbjct: 555 GGIITSVSKVLSEDSEV----RAIIETPQLGPGSFSVLTPWTSGLDMKKRMARYSRTASL 614

Query: 655 FALARDKGSGTIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSIN 714
             + RD+GSG +K+    +Y +D  D +NL+ GL ++L+IL AAGAEE+GTH + G+ + 
Sbjct: 615 ITIVRDRGSGEVKTEGRINYTVDKTDRDNLKAGLRESLRILIAAGAEEVGTHRSDGQRLI 674

Query: 715 VKKVSYREYEKFV----KEESSRPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWE 774
            K V+    ++F+     EE ++ + +      SAHQMGSCR+G   K+  ++  GE+WE
Sbjct: 675 CKGVNENSIQEFLDSVSTEEGAKGMTEKWNVYSSAHQMGSCRIGENEKEGAIDLNGESWE 734

Query: 775 VEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSVLETL 792
            E LF+ D+S  P+A+GVNPM+TV + AYC +  + +++
Sbjct: 735 AEKLFVCDASALPSAVGVNPMITVMSTAYCISTRIAKSM 740

BLAST of Lcy12g013550 vs. TAIR 10
Match: AT1G03990.1 (Long-chain fatty alcohol dehydrogenase family protein )

HSP 1 Score: 496.5 bits (1277), Expect = 4.0e-140
Identity = 286/772 (37.05%), Postives = 446/772 (57.77%), Query Frame = 0

Query: 50  SNNSSLSAAEMESLAALCDTFLPSVAAAD----------RSAAV--FFETSASTADT-PE 109
           S +   S +++++L+++CD  +P V              R+ A+  FF++S+S +   P+
Sbjct: 23  SFSHGFSKSDLQALSSICDAIMPPVPLESLNLEMKLKVLRNDALLSFFKSSSSESHVRPD 82

Query: 110 RVGEMIKERVRHPKKWLARLSLWMLSTWYGTYIMCGNKSLSNK-FPYFQRFSKVSQRRRE 169
            V E++  +       + R+ L +L+   GT ++CG   L  K +P+  +FS++S  +RE
Sbjct: 83  EVAELLATKAIPLTVLVVRIVLRILTFRLGTLLLCGLVCLDKKHWPFLLKFSEMSLEKRE 142

Query: 170 QIVVSWSLSYFF-LLRMFFRTVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTR 229
           +++  W+  ++  L R+ F  +K   L  +FT  ++   N  W+AI+Y            
Sbjct: 143 KVLQRWNTQWYNPLARIGFMMIKAIFLFYYFTWTNENSENPVWDAINY------------ 202

Query: 230 QSEASNLKENGRSREVEEDEEELYGPLFKGIV-NLEHQQVAAAEALRKFGFSVTLLSPRT 289
              +  + EN       ED E+   PL +GI+   +  ++   + +   G  VT      
Sbjct: 203 ---SVEIGEN-------EDMEQKERPLDEGIIETAKEDEMTIKQRMINKGLKVT-----E 262

Query: 290 KASSPSMIIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEE 349
                +  I+CDAVVVGSG GGGV A +LA +G +V+V+EKG+Y A  + S LEG +M E
Sbjct: 263 DRERDTYKIECDAVVVGSGCGGGVAAAILAKSGLRVVVIEKGNYFAPRDYSALEGPSMFE 322

Query: 350 MYLSSGLVATDNMNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYK 409
           ++ S+ L+ T +     +AGSTVGGGS VNW+AS++TP  +  EWS    + ++SS  YK
Sbjct: 323 LFESNSLMMTHDGRFRFMAGSTVGGGSVVNWAASLKTPDAIIEEWSVHRGISIYSSEKYK 382

Query: 410 EAMEVVCKKMGVQSEIDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNG 469
            AM +VCK++GV  +I  EGF N +LRKGCE+LG  V  +PRNS   HYCG C  GC  G
Sbjct: 383 AAMGIVCKRLGVTEKIIREGFQNQILRKGCEKLGLDVTIVPRNSTEKHYCGSCSYGCPTG 442

Query: 470 RKKGTAETWLVDLVRSGNGVILPGCEAIDI------RQEKKDGSERNRAKGVAFEFGCGS 529
            K+GT  TWLVD V + N VIL  C+A  +        ++++   R R  GVA       
Sbjct: 443 EKRGTDSTWLVDAV-NNNAVILTQCKAEKLILADNDANKREESGRRKRCLGVAASL--SH 502

Query: 530 GAKQMFLVESKVTVVACGALSTPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSW 589
             ++   + +KVT+VACG+L TP LL  SGL+N NI + LH+HP+ MAWG+FP +     
Sbjct: 503 QTRKKLQINAKVTIVACGSLKTPGLLASSGLKNSNISRGLHIHPIMMAWGYFPEKNS--- 562

Query: 590 PEEDKKSYEGGIMTAMCNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRM 649
            E +  ++EG I+T++  +       P      ++TPA+ PG F+ L PW+SG+++K+RM
Sbjct: 563 -ELEGAAHEGEIVTSLHYVHPMDSTTP---NITLETPAIGPGTFAALTPWVSGSDMKERM 622

Query: 650 CKFSRTAHVFALARDKGSGTIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGT 709
            K++RTAH+FA+ RD+G G +K      Y +   DEENL  GL++AL+IL AAGA E+GT
Sbjct: 623 AKYARTAHIFAMVRDEGVGEVKG-DIVKYRLTKADEENLTIGLKQALRILVAAGAAEVGT 682

Query: 710 HHNKGRSINVKKVSYREYEKFVKEESSRP----LKDLMTPICSAHQMGSCRMGIRAKDSV 769
           + + G+ +    +  ++ E F+   ++ P    +    T   +AHQ+G CRMG   K+  
Sbjct: 683 YRSDGQRMKCDGIKQKDLEAFLDTVNAPPGVVSMSKHWTQSFTAHQIGCCRMGATEKEGA 742

Query: 770 VNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSVLETLKRRR 796
           ++ +GE+WE E L++ D+SV PTALGVNPM+TVQ+ AYC +  + E +K+R+
Sbjct: 743 IDGKGESWEAEDLYVCDASVLPTALGVNPMITVQSTAYCISNRIAELMKKRK 756