Homology
BLAST of Lcy12g013550 vs. ExPASy Swiss-Prot
Match:
O65709 (Long-chain-alcohol oxidase FAO4A OS=Arabidopsis thaliana OX=3702 GN=FAO4A PE=3 SV=2)
HSP 1 Score: 866.3 bits (2237), Expect = 2.7e-250
Identity = 438/744 (58.87%), Postives = 560/744 (75.27%), Query Frame = 0
Query: 60 MESLAALCDTFLPS-----VAAADRSAAVFFETSASTADTPERVGEMIKERVRHPKKWLA 119
MESL A+CDTF+ S V D A +F SAS TP+RV ++ ER+ HPKKW+
Sbjct: 1 MESLVAICDTFISSIDDSGVGHVDDCVAGYFSASASQTGTPDRVARLMSERLHHPKKWIL 60
Query: 120 RLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFLLRMFFR 179
R LW+LSTW G+ ++CG +S + +FPYF+RF ++ ++RRE+I+++WS SYF LLRM FR
Sbjct: 61 RAGLWLLSTWIGSLVLCGWRSFTGEFPYFRRFCRLPEKRREEILLNWSSSYFSLLRMLFR 120
Query: 180 TVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKENGRSREVEEDE 239
T+K+ L FFTQVD+K N++W AI Y G P L E + ++
Sbjct: 121 TIKLISALVFFTQVDEKGRNLAWKAIGYNGPSPD-----HSDHEVELNEEKKKKK----P 180
Query: 240 EELYGPLFKGIVNLEHQQVAAAEALRKFGFSVTLLSPRTKASS---PSMIIKCDAVVVGS 299
EE++GPL+ GIV+L+ + A + L GF+V+ T SS P M I+CDAVVVGS
Sbjct: 181 EEIFGPLYNGIVDLKSPREAVEKKLAGRGFAVSNQKRNTNGSSISDPVMKIQCDAVVVGS 240
Query: 300 GSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDNMNVLVL 359
GSGGGV AGVLA AGYKVLV+E G+Y AR+ LSLLEGQAM++MYLS GL+AT + NV++L
Sbjct: 241 GSGGGVAAGVLAKAGYKVLVIESGNYYARSKLSLLEGQAMDDMYLSGGLLATSDTNVVIL 300
Query: 360 AGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGVQSEIDN 419
AGSTVGGGST+NWSASI+TP HV +EW+++ +LE+F S LY+EAM+VVCK+MGVQ
Sbjct: 301 AGSTVGGGSTINWSASIKTPEHVMKEWAEKSKLEMFGSDLYREAMDVVCKRMGVQCGFVE 360
Query: 420 EGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGN 479
EGFNN VLRKGCE+LG V NIPRN+PSDHYCG+CCLGCK G+K+GT+ETWLVDLV S N
Sbjct: 361 EGFNNEVLRKGCEKLGLPVKNIPRNAPSDHYCGFCCLGCKKGQKQGTSETWLVDLVESDN 420
Query: 480 GVILPGCEAIDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVTVVACGALSTPP 539
G+ILPGC+A ++ + + G ++ +A GVAF FG +++++VES+VT+VACGAL TP
Sbjct: 421 GLILPGCQATEVMYDCEQG-KKKKATGVAFAFG-----EEIYVVESRVTIVACGALRTPH 480
Query: 540 LLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAMCNIGSEPD 599
LL++SGL+N NIG+NL LHPV MAWG FP E DK WPE+ KKSYEGGIMTAM ++ E +
Sbjct: 481 LLKRSGLKNSNIGRNLCLHPVVMAWGWFP-EEDK-WPEKKKKSYEGGIMTAMSSVVIE-E 540
Query: 600 QEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDKGSGTIKSP 659
YG VIQTPALHPG+FS ++PW S + K RM KFSRTAH+FAL RDKG+GTI S
Sbjct: 541 THSSYGEMVIQTPALHPGMFSGIIPWTSSKDFKTRMLKFSRTAHIFALLRDKGTGTIDSK 600
Query: 660 SSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYEKFVKE 719
+ YN++D DEE+L+ GLE+ LKILAAAGAEEIGTHH++GRS+NV+ S E E+FV+E
Sbjct: 601 TYIDYNLNDEDEESLKNGLERVLKILAAAGAEEIGTHHSEGRSLNVRTASSLEIERFVRE 660
Query: 720 ESSRPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIADSSVFPTALGVN 779
ESS+PLKDL ICSAHQMGSCRMGIR ++S V GETWEVE LF+AD+SVFPTALGVN
Sbjct: 661 ESSKPLKDLSGQICSAHQMGSCRMGIRPEESAVRPTGETWEVERLFVADTSVFPTALGVN 720
Query: 780 PMVTVQAIAYCTAQSVLETLKRRR 796
PMVTVQ+IAYC +V++ LK+++
Sbjct: 721 PMVTVQSIAYCIGLNVVDVLKKKK 726
BLAST of Lcy12g013550 vs. ExPASy Swiss-Prot
Match:
B5WWZ8 (Long-chain-alcohol oxidase FAO1 OS=Lotus japonicus OX=34305 GN=FAO1 PE=1 SV=1)
HSP 1 Score: 562.4 bits (1448), Expect = 8.3e-159
Identity = 315/765 (41.18%), Postives = 456/765 (59.61%), Query Frame = 0
Query: 49 NSNNSSLSAAEMESLAALCDTFLPSVA-----------AADRSAAVFFETSASTADTPER 108
N S++EMESLA++C+ LP + + F+ TSAS P
Sbjct: 16 NKFRQGFSSSEMESLASICEVILPPLPMDALKIKKHDDVSIEDVEFFWNTSASHYPIPHE 75
Query: 109 VGEMIKERVRHPKKWLARLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQI 168
V E++ +R L R+ LW+L+T GT+++CG S K+P+ FS +S ++RE++
Sbjct: 76 VAEILSKRSLIEAVILIRVVLWLLATRLGTFLLCGLLCFSEKWPFINNFSSMSLKKRERV 135
Query: 169 VVSWSLSYFFL-LRMFFRTVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQS 228
+ W + F +R+ F +K+ CL A+FT VD+ +N +W AI Y +
Sbjct: 136 MQWWLKNRFITPIRLAFAYLKVLCLFAYFTWVDENGDNPAWKAIGY---------EVSAD 195
Query: 229 EASNLKENGRSREVEEDEEELYGPLFKGIVNLEHQ-QVAAAEALRKFGFSVTLLSPRTKA 288
E NGR E KGIV H+ ++L + G +VTL +
Sbjct: 196 EKLPNSSNGRLLE-------------KGIVETMHETNSTLQQSLTEKGLNVTL-----DS 255
Query: 289 SSPSMIIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMY 348
+ + +KCDA+VVGSG GGGV A VL+ AG+KV+VLEKG+Y A + S LEG +M+++Y
Sbjct: 256 RNNILKVKCDAIVVGSGCGGGVAASVLSGAGHKVVVLEKGNYFAPRDYSSLEGPSMDQLY 315
Query: 349 LSSGLVATDNMNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEA 408
+ G++A+ + +L+LAGSTVGGGS VNWSA I+TP V +EWS+ + L LFS+S Y A
Sbjct: 316 ETGGILASVDSRILLLAGSTVGGGSAVNWSACIKTPHEVLKEWSENHNLSLFSTSEYLSA 375
Query: 409 MEVVCKKMGVQSEIDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRK 468
ME VC+++GV +EGF N VLRKGC+ LG V +PRNS +H+CG C GC G K
Sbjct: 376 METVCERIGVTESCTHEGFQNQVLRKGCQNLGLKVDYVPRNSSGNHFCGSCGYGCPKGEK 435
Query: 469 KGTAETWLVDLVRSGNGVILPGCEAID-IRQEKKDGSERNRAKGVAFEFGCGSGAKQMFL 528
+GT +TWLVD V +G VI+ GC+A + + ++GS R + S
Sbjct: 436 QGTQDTWLVDAVENG-AVIITGCKAERFLLENNRNGSGRKKKCLGVLAKALSSRVTMKLQ 495
Query: 529 VESKVTVVACGALSTPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKS 588
+E+KVT+ A GAL TPPL+ SGL+N NIGKNLHLHPV M WG+FP T E K
Sbjct: 496 IEAKVTISAGGALLTPPLMISSGLKNKNIGKNLHLHPVLMTWGYFPESTS----ELKGKI 555
Query: 589 YEGGIMTAMCNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTA 648
+EGGI+T++ + S A+I+TP+L PG F+ L PW SG + K+RM + RTA
Sbjct: 556 FEGGIITSVHKVPSRDSNSYSDTRAIIETPSLGPGSFASLCPWESGLDFKERMLNYPRTA 615
Query: 649 HVFALARDKGSGTIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRS 708
H+ + RDK G + + SY + D+EN++ GL++AL+IL AAGA E+GTH + G+
Sbjct: 616 HLITIIRDKACGQVTTEGRVSYKLSAFDKENMKCGLQQALRILKAAGAVEVGTHRSDGQR 675
Query: 709 INVKKVSYREYEKFVKEESSRPLKDLMTP------ICSAHQMGSCRMGIRAKDSVVNQRG 768
+ +VS E +F+ +S P++ ++P SAHQMGSCRMG+ K+ V++ G
Sbjct: 676 VKCGEVSENEMAEFI--DSVCPMEGALSPGENWNIYTSAHQMGSCRMGMNEKEGAVDENG 735
Query: 769 ETWEVEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSVLETLKR 794
E+WE +GLF+ D+S+ PTA+GVNPM+T+Q+ AYC + V++ LKR
Sbjct: 736 ESWEAQGLFVCDASLLPTAVGVNPMITIQSTAYCISNRVVDYLKR 746
BLAST of Lcy12g013550 vs. ExPASy Swiss-Prot
Match:
B5WWZ9 (Long-chain-alcohol oxidase FAO2 OS=Lotus japonicus OX=34305 GN=FAO2 PE=2 SV=1)
HSP 1 Score: 537.0 bits (1382), Expect = 3.7e-151
Identity = 303/761 (39.82%), Postives = 452/761 (59.40%), Query Frame = 0
Query: 55 LSAAEMESLAALCDTFLPS--------VAAADRSAAVFFETSASTADTPERVGEMIKERV 114
LS+++M +AA+C+ PS ++ D++ + F+ S S A P+ E++ +
Sbjct: 27 LSSSQMHVIAAICEALFPSQPLDSQNNQSSVDKALSAFYTASGSQAPLPDEAAELLFKFN 86
Query: 115 RHPKKWLARLS--LWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLS 174
R + L+ +S L +LS GT ++CG L ++P+ +FS++ +RE+I+ WS
Sbjct: 87 RSFPEALSLVSWVLLILSFRLGTLLLCGTLCLDWRWPFIHKFSEIPLEKREEILKRWSRE 146
Query: 175 YFFL-LRMFFRTVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKE 234
++ LR+ F K+ C F++ D +N W AI Y + TR+ L +
Sbjct: 147 KCWIPLRLVFVLTKLVCFYNLFSRADVNGHNPIWKAIGY-------QVDTRE----KLTQ 206
Query: 235 NGRSREVEEDEEELYGPLFKGIVNLEHQ-QVAAAEALRKFGFSVTLLSPRTKASSPSMII 294
R PL +G++ ++ ++L + G VT I
Sbjct: 207 KKR-------------PLQEGLIETMYETDSTLIQSLTEKGLEVT-----EDLEQNMYKI 266
Query: 295 KCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVA 354
KCDAV+VGSG GGGV A VLAN+G+KV++LEKG Y + S LEG +M E+Y S G++
Sbjct: 267 KCDAVIVGSGCGGGVAAAVLANSGHKVIILEKGEYFVSHDYSSLEGPSMNELYESGGILP 326
Query: 355 TDNMNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKK 414
+ + +++LAGST+GG S +NWSA I+TP V REWS+++++ LF+S Y+ AM+ VC++
Sbjct: 327 SLDGKMMILAGSTLGGXSAINWSACIRTPDSVLREWSEKHKIPLFASPDYQSAMDTVCRR 386
Query: 415 MGVQSEIDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETW 474
+GV + E F N +LR+GC ++G V + NS +DHYCG CC GC+ G KKGT TW
Sbjct: 387 IGVTENCNKESFQNQILRQGCAKIGFKVEPVAINSSADHYCGSCCYGCRTGDKKGTESTW 446
Query: 475 LVDLVRSGNGVILPGCEAIDIRQEKKD---GSERNRAKGVAFEFGCGSGAKQMFLVESKV 534
LVD V +G VIL GC+A + KD G++R GV S + +ESKV
Sbjct: 447 LVDAVGNG-AVILTGCKAEKLNFTLKDGDNGTKRKTCSGVIASATWRSKVTKKLQIESKV 506
Query: 535 TVVACGALSTPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGI 594
T+ ACG+LSTPPL+ SGL+NPNIGKNLHLHP AWG+FP + +YEGGI
Sbjct: 507 TISACGSLSTPPLMISSGLKNPNIGKNLHLHPCQFAWGYFPEDMTNF----SGNNYEGGI 566
Query: 595 MTAMCNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFAL 654
+T++ + E +I+ PAL PG FS L+PW+SG ++K+RM K++RTA++FAL
Sbjct: 567 ITSIHKVFEE--DSTSTPRIIIEAPALGPGSFSALVPWVSGLDVKERMVKYARTANLFAL 626
Query: 655 ARDKGSGTIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKK 714
RD GSG +K+ SY +D +D E+LQ GL KAL+IL AAGA E+GT+ + G+ I +
Sbjct: 627 VRDHGSGEVKAEGRISYKLDKIDRESLQTGLRKALRILVAAGAVEVGTYRSDGQRIKCRG 686
Query: 715 VSYREYEKF------VKEESSRPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWEV 774
+ + E+F V SSR ++ T SAHQM SCRM ++ V++ GE+WE
Sbjct: 687 IKESDLEEFLDSVRVVGGPSSR--NEVWTVFTSAHQMTSCRMSATEEEGAVDENGESWEA 746
Query: 775 EGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSVLETLKRR 795
+GL++ D SV P+A+GVNPM+T+Q+ AYC A ++ E+LK++
Sbjct: 747 KGLYVCDGSVLPSAVGVNPMITIQSTAYCIASNIAESLKKQ 749
BLAST of Lcy12g013550 vs. ExPASy Swiss-Prot
Match:
Q94BP3 (Long-chain-alcohol oxidase FAO4B OS=Arabidopsis thaliana OX=3702 GN=FAO4B PE=2 SV=2)
HSP 1 Score: 525.0 bits (1351), Expect = 1.5e-147
Identity = 305/755 (40.40%), Postives = 441/755 (58.41%), Query Frame = 0
Query: 52 NSSLSAAEMESLAALCDTFLPSVAAADRSAAVFFETSASTADT-PERVGEMIKERVRHPK 111
N S ++++SLA +C TFLP +++ A F ++ST + V EMI + R
Sbjct: 24 NHGFSPSQIQSLAVICQTFLPPETTSEQQAVNSFHVASSTQPPFTDEVAEMIVKNGRSEA 83
Query: 112 KWLARLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFLL- 171
+ R+ L +LS +GT ++CG+ L +P+ +FS++ +RE I+ +WS FLL
Sbjct: 84 VKVLRIILMILSFRFGTLLLCGSLCLDKSWPFVLKFSQLPLDKREAILRNWSRQSGFLLP 143
Query: 172 -RMFFRTVKIFCLLAFFTQVDDKMNNISWNAIDY--PGTDPQFKAKTRQSEASNLKENGR 231
R+ F K + L FF+Q D+ + N + AI Y GT+ R
Sbjct: 144 FRITFFLAKFYTLFYFFSQTDENLKNPALEAIGYCIDGTE-------------------R 203
Query: 232 SREVEEDEEELYGPLFKGIVNLEHQ-QVAAAEALRKFGFSVTLLSPRTKASSPSMIIKCD 291
S + + +E PL KGI+ H+ V ++L + G V I+CD
Sbjct: 204 SSNKKSEADEKRRPLEKGIIETMHESDVTITQSLTEKGVHVA-----RDDGDNVYRIRCD 263
Query: 292 AVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDN 351
AVVVGSGSGGGV A LA AG KVLVLEKG+Y + S LE +M E+Y GL+ T +
Sbjct: 264 AVVVGSGSGGGVAAANLAKAGLKVLVLEKGNYFTAHDYSGLEVPSMLELYEKGGLLTTVD 323
Query: 352 MNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGV 411
++LAGS VGGG+ VNWSASI+TP HV +EWS+ +++ F S Y+ AM+ V ++GV
Sbjct: 324 GKFMLLAGSAVGGGTAVNWSASIRTPDHVLQEWSEGSKIKFFGSQEYQSAMDEVTIRIGV 383
Query: 412 QSEIDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVD 471
GF N VLRKGCE LG V ++PRNSP DHYCG C GC+ G K GT +TWLVD
Sbjct: 384 TERCVKHGFQNQVLRKGCERLGLQVESVPRNSPEDHYCGLCGYGCRAGAKNGTDQTWLVD 443
Query: 472 LVRSGNGVILPGCEAIDIRQEKKDGSERNRAKGVAFEFGCGSGAK--QMFLVESKVTVVA 531
V +G VIL G +A S R K F G K + F++E++VTV +
Sbjct: 444 AVENG-AVILTGIKAERFVLVDNTSSSNERKKRCVGVFASSVGGKIGKKFIIEARVTVSS 503
Query: 532 CGALSTPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAM 591
G+L TPPL+ SGL+NPNIG+NL LHPV M WG+FP E D E K YEGGI+T++
Sbjct: 504 AGSLLTPPLMLSSGLKNPNIGRNLKLHPVLMTWGYFP-EKDS---EFSGKMYEGGIITSV 563
Query: 592 CNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDK 651
++ D E G A+++ P + P ++ L PW+SG ++K+RM K+ RTAH+FAL RD
Sbjct: 564 HHMN---DTESGC-KAILENPLIGPASYAGLSPWVSGPDLKERMIKYGRTAHLFALVRDL 623
Query: 652 GSGTIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYR 711
GSG + + +Y D ENL+ GL +AL++ AAGA E+GT+ + G+ + + ++
Sbjct: 624 GSGEVMMENEVTYRTTKKDRENLRAGLRQALRVSVAAGAVEVGTYRSDGQKMKCEAITKE 683
Query: 712 EYEKFVKEESS----RPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIA 771
E+F+ E + + T SAHQMGSCRMG+ A++ +++ GE+WE EGLF+
Sbjct: 684 AMEEFLDEVDAVGGVGTKGEYWTTYFSAHQMGSCRMGVTAEEGALDENGESWEAEGLFVC 743
Query: 772 DSSVFPTALGVNPMVTVQAIAYCTAQSVLETLKRR 795
D S+ P+A+GVNPM+T+Q+ AYC + ++++L+ +
Sbjct: 744 DGSILPSAVGVNPMITIQSTAYCISSKIVDSLQNK 745
BLAST of Lcy12g013550 vs. ExPASy Swiss-Prot
Match:
Q9LW56 (Long-chain-alcohol oxidase FAO3 OS=Arabidopsis thaliana OX=3702 GN=FAO3 PE=1 SV=1)
HSP 1 Score: 522.7 bits (1345), Expect = 7.3e-147
Identity = 307/759 (40.45%), Postives = 447/759 (58.89%), Query Frame = 0
Query: 55 LSAAEMESLAALCDTFLPSVAAADRSAA-------------VFFETSASTADTPERVGEM 114
++ AEMESLA+ C+ LPSV + F+ TS S + E+
Sbjct: 15 ITVAEMESLASFCEAVLPSVQPPPEELSGEGDNHRNKEALRSFYSTSGSKTPVLRQSIEL 74
Query: 115 IKERVRHPKKWLARLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSW 174
+ +R RL L++L+T GT ++CG + L +++P+ ++FS++S +RE+++
Sbjct: 75 VTKRGTIEAYIATRLILFLLATRLGTLLICGTECLVSRWPFVEKFSELSLEKRERVLQKQ 134
Query: 175 SLSYFFL-LRMFFRTVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASN 234
++ +R F +K+ L FF++V+ N +W AI Y +P + + SE N
Sbjct: 135 FKNWILTPIRAAFVYIKVAFLFCFFSRVNPNGENPAWEAIGY-RVNPD---ENKPSETHN 194
Query: 235 LKENGRSREVEEDEEELYGPLFKGIV-NLEHQQVAAAEALRKFGFSVTLLSPRTKASSPS 294
+ PL KGIV +E + E+L G L +
Sbjct: 195 ER-----------------PLEKGIVETMEETEQTLLESLAHKGLEAVL-----DTEHDA 254
Query: 295 MIIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSG 354
+ IKCD VVVGSGSGGGV A VLA +G KV+VLEKGSY + EG ++++Y + G
Sbjct: 255 IRIKCDVVVVGSGSGGGVAASVLAKSGLKVVVLEKGSYFTPSEHRPFEGPGLDKLYENGG 314
Query: 355 LVATDNMNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVV 414
++ + + + +VLAG+TVGGGS VNWSA I+TP V +EWS++ + LF + Y AMEVV
Sbjct: 315 ILPSVDGSFMVLAGATVGGGSAVNWSACIKTPKSVLQEWSEDQNIPLFGTKEYLTAMEVV 374
Query: 415 CKKMGVQSEIDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTA 474
K+MGV + + E F N +LRKGCE LG +V N+PRNS HYCG C GC+ G KKG+
Sbjct: 375 WKRMGVTEKCELESFQNQILRKGCENLGFNVENVPRNSSESHYCGSCGYGCRQGDKKGSD 434
Query: 475 ETWLVDLVRSGNGVILPGCEAIDIRQEK---KDGSERNRAKGVAFEFGCGSGAKQMFLVE 534
TWLVD V G VIL GC+A EK G ++ + GV + G+ AK M +E
Sbjct: 435 RTWLVDAVGHG-AVILTGCKAERFILEKNGSNKGGKQMKCLGVMAKSLNGNIAK-MLKIE 494
Query: 535 SKVTVVACGALSTPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYE 594
+KVTV A GAL TPPL+ SGLRN NIGKNLHLHPV MAWG+FP + + S SYE
Sbjct: 495 AKVTVSAGGALLTPPLMISSGLRNRNIGKNLHLHPVLMAWGYFP-DKESSNISFKGNSYE 554
Query: 595 GGIMTAMCNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHV 654
GGI+T++ + SE + A+I+TP L PG FS+L PW SG ++K+RM ++SRTA +
Sbjct: 555 GGIITSVSKVLSEDSEV----RAIIETPQLGPGSFSVLTPWTSGLDMKKRMARYSRTASL 614
Query: 655 FALARDKGSGTIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSIN 714
+ RD+GSG +K+ +Y +D D +NL+ GL ++L+IL AAGAEE+GTH + G+ +
Sbjct: 615 ITIVRDRGSGEVKTEGRINYTVDKTDRDNLKAGLRESLRILIAAGAEEVGTHRSDGQRLI 674
Query: 715 VKKVSYREYEKFV----KEESSRPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWE 774
K V+ ++F+ EE ++ + + SAHQMGSCR+G K+ ++ GE+WE
Sbjct: 675 CKGVNENSIQEFLDSVSTEEGAKGMTEKWNVYSSAHQMGSCRIGENEKEGAIDLNGESWE 734
Query: 775 VEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSVLETL 792
E LF+ D+S P+A+GVNPM+TV + AYC + + +++
Sbjct: 735 AEKLFVCDASALPSAVGVNPMITVMSTAYCISTRIAKSM 740
BLAST of Lcy12g013550 vs. ExPASy TrEMBL
Match:
A0A6J1FFC0 (Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111443434 PE=3 SV=1)
HSP 1 Score: 1387.9 bits (3591), Expect = 0.0e+00
Identity = 699/795 (87.92%), Postives = 737/795 (92.70%), Query Frame = 0
Query: 1 MGYFDATAAASCGGDGEAKRQLDHCVIDLHGGADRLHDGAPAGISQHANSNNSSLSAAEM 60
MGYF AAASCGGDGE+K QL CVI+L+GGAD LHDG PAGISQHAN N SLSAAEM
Sbjct: 12 MGYFG--AAASCGGDGESKPQLHQCVIELNGGADSLHDGGPAGISQHANF-NYSLSAAEM 71
Query: 61 ESLAALCDTFLPSVAAADRSAAVFFETSASTADTPERVGEMIKERVRHPKKWLARLSLWM 120
ESLAALCDTFLPSVAA D SA+ FFETSAS A TPERVG MIKERVRHPKKWL R+SLWM
Sbjct: 72 ESLAALCDTFLPSVAATDGSASEFFETSASAAGTPERVGGMIKERVRHPKKWLMRVSLWM 131
Query: 121 LSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFLLRMFFRTVKIFC 180
LSTWYGTYI+CGN SLSN FPYFQRFSKVSQ++REQ+VVSWSLSYF LLRMFFRT+KI C
Sbjct: 132 LSTWYGTYILCGNGSLSNHFPYFQRFSKVSQKKREQVVVSWSLSYFLLLRMFFRTIKILC 191
Query: 181 LLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKENGRSREVEEDEEELYGP 240
LAFFTQVD++MNNISW AIDYPG+DPQFKAK RQSE NLKE RSRE+ E+EEELYGP
Sbjct: 192 HLAFFTQVDEEMNNISWKAIDYPGSDPQFKAKARQSEEWNLKEKVRSREIVEEEEELYGP 251
Query: 241 LFKGIVNLEHQQVAAAEALRKFGFSVTLLSPRTKASSPSMIIKCDAVVVGSGSGGGVVAG 300
LFKGIVNLEHQQVA ALRKFGF VTLLSP K+ SPSMIIKCDAVVVGSGSGGGVVAG
Sbjct: 252 LFKGIVNLEHQQVAVG-ALRKFGFPVTLLSPSIKSPSPSMIIKCDAVVVGSGSGGGVVAG 311
Query: 301 VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDNMNVLVLAGSTVGGGS 360
VLA+AGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGLVATDNMNVL+LAGSTVGGGS
Sbjct: 312 VLASAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNVLILAGSTVGGGS 371
Query: 361 TVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGVQSEIDNEGFNNAVLR 420
TVNWSASIQTPSHVTREWSDEYELELF S+LYKEA+EVVCK+MGVQSEIDNEGFNNAVLR
Sbjct: 372 TVNWSASIQTPSHVTREWSDEYELELFGSNLYKEALEVVCKRMGVQSEIDNEGFNNAVLR 431
Query: 421 KGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGVILPGCEA 480
KGCEELG +NIPRNS SDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNG ILPGCEA
Sbjct: 432 KGCEELGCKAINIPRNSASDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA 491
Query: 481 IDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVTVVACGALSTPPLLRKSGLRN 540
I I QE KDG +RNRAKGVAFEFGCGSGAKQM LVESKVT+VACGAL TP LL++SGLRN
Sbjct: 492 IRITQE-KDGRKRNRAKGVAFEFGCGSGAKQMCLVESKVTIVACGALCTPQLLKRSGLRN 551
Query: 541 PNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAMCNIGSEPDQEPGYGGAV 600
PNIGKNLHLHPVTMAWG+FPAETD+SWPE+DKKSYEGGIMTAMCNIGS PDQEPGYGGAV
Sbjct: 552 PNIGKNLHLHPVTMAWGYFPAETDESWPEKDKKSYEGGIMTAMCNIGSSPDQEPGYGGAV 611
Query: 601 IQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDKGSGTIKSPSSFSYNMDD 660
IQTPALHPGLFSILMPW+SGTNIKQRMCKFSRTAHVFALARDKGSGT++S SS SY MDD
Sbjct: 612 IQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFALARDKGSGTVESSSSLSYIMDD 671
Query: 661 VDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYEKFVKEESSRPLKDL 720
DE+NLQKGLEKALKIL AAGAEEIGTHHNKGRSINVKKVSYREYE+FVKEESSRPLKDL
Sbjct: 672 GDEKNLQKGLEKALKILGAAGAEEIGTHHNKGRSINVKKVSYREYERFVKEESSRPLKDL 731
Query: 721 MTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA 780
+TPICSAHQMGSCRMGI AKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA
Sbjct: 732 LTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA 791
Query: 781 YCTAQSVLETLKRRR 796
YCTA+SVLETLKR+R
Sbjct: 792 YCTAKSVLETLKRKR 801
BLAST of Lcy12g013550 vs. ExPASy TrEMBL
Match:
A0A6J1IPF0 (Long-chain-alcohol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111477017 PE=3 SV=1)
HSP 1 Score: 1370.9 bits (3547), Expect = 0.0e+00
Identity = 692/795 (87.04%), Postives = 732/795 (92.08%), Query Frame = 0
Query: 1 MGYFDATAAASCGGDGEAKRQLDHCVIDLHGGADRLHDGAPAGISQHANSNNSSLSAAEM 60
MGYF AA+S GGDGE+K QL CVI+L+G AD LHDG PAGISQHANS N SLSAAEM
Sbjct: 12 MGYFG--AASSYGGDGESKPQLHQCVIELNGCADSLHDGGPAGISQHANS-NYSLSAAEM 71
Query: 61 ESLAALCDTFLPSVAAADRSAAVFFETSASTADTPERVGEMIKERVRHPKKWLARLSLWM 120
ESLAALC+TFLPSVAA D SA+ FFETSAS A T ERVG MIKERVRHPKKWL R+SLWM
Sbjct: 72 ESLAALCETFLPSVAATDGSASEFFETSASAAGTSERVGGMIKERVRHPKKWLMRMSLWM 131
Query: 121 LSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFLLRMFFRTVKIFC 180
LSTWYGTYI+CGN SLSN FPYFQRFSKVSQ++REQ+VVSWSLSYF LLRMFFRT+KI
Sbjct: 132 LSTWYGTYILCGNGSLSNHFPYFQRFSKVSQKKREQVVVSWSLSYFLLLRMFFRTIKILS 191
Query: 181 LLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKENGRSREVEEDEEELYGP 240
LAFFTQVD++MNNISW AIDYPG+DPQFKAK RQSE NLKE RSRE+EE+EEELYGP
Sbjct: 192 HLAFFTQVDEEMNNISWKAIDYPGSDPQFKAKARQSEEWNLKEKVRSREIEEEEEELYGP 251
Query: 241 LFKGIVNLEHQQVAAAEALRKFGFSVTLLSPRTKASSPSMIIKCDAVVVGSGSGGGVVAG 300
LFKGIVNLEH QV A ALRKFGF VTLLSP TK+ SPSMIIKCDAVVVGSGSGGGVV G
Sbjct: 252 LFKGIVNLEH-QVVAVGALRKFGFPVTLLSPSTKSPSPSMIIKCDAVVVGSGSGGGVVGG 311
Query: 301 VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDNMNVLVLAGSTVGGGS 360
VLA+AGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGLVATDNMNVL+LAGSTVGGGS
Sbjct: 312 VLASAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNVLILAGSTVGGGS 371
Query: 361 TVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGVQSEIDNEGFNNAVLR 420
TVNWSASIQTPSHVTREWSDEYELELF S+LYKEA+EVVCK+MGVQSEIDNEGFNNA+LR
Sbjct: 372 TVNWSASIQTPSHVTREWSDEYELELFRSNLYKEALEVVCKRMGVQSEIDNEGFNNAILR 431
Query: 421 KGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGVILPGCEA 480
KGCEELG +NIPRNS SDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNG ILPGCEA
Sbjct: 432 KGCEELGCKAINIPRNSASDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA 491
Query: 481 IDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVTVVACGALSTPPLLRKSGLRN 540
I I QE KDG ERNRAKGVAFEF CGS AKQM LVESKVT+VACGAL TPPLL++SGLRN
Sbjct: 492 IRITQE-KDGRERNRAKGVAFEFECGSEAKQMCLVESKVTIVACGALCTPPLLKRSGLRN 551
Query: 541 PNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAMCNIGSEPDQEPGYGGAV 600
PNIGKNLHLHPV MAWG+FPAETD+SWPE+DKKSYEGGIMTAMCNIGS PDQEPGYGGAV
Sbjct: 552 PNIGKNLHLHPVAMAWGYFPAETDESWPEKDKKSYEGGIMTAMCNIGSSPDQEPGYGGAV 611
Query: 601 IQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDKGSGTIKSPSSFSYNMDD 660
IQTPALHPGLFSILMPW+SGTNIKQRMCKFSRTAHVFALARDKGSGT++S SS SY MDD
Sbjct: 612 IQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFALARDKGSGTVESSSSLSYIMDD 671
Query: 661 VDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYEKFVKEESSRPLKDL 720
DE+NLQKGLEKALKIL AAGAEEIGTHHNKGRSINVKKVSYREYE+FVKEESSRPLKDL
Sbjct: 672 GDEKNLQKGLEKALKILGAAGAEEIGTHHNKGRSINVKKVSYREYERFVKEESSRPLKDL 731
Query: 721 MTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA 780
+TPICSAHQMGSCRMGI AKDSVVNQRGETWEVEGLF+ADSSVFPTALGVNPMVTVQAIA
Sbjct: 732 LTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFVADSSVFPTALGVNPMVTVQAIA 791
Query: 781 YCTAQSVLETLKRRR 796
YCTAQSVLETLKR+R
Sbjct: 792 YCTAQSVLETLKRKR 801
BLAST of Lcy12g013550 vs. ExPASy TrEMBL
Match:
A0A6J1FQT3 (Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111446403 PE=3 SV=1)
HSP 1 Score: 1366.3 bits (3535), Expect = 0.0e+00
Identity = 699/811 (86.19%), Postives = 738/811 (91.00%), Query Frame = 0
Query: 1 MGYFDATAAA--------SC----GGDGEAKRQLDHCVIDLHGGADRLHDGAPAGISQHA 60
MGYF A AAA SC GGD EAKR+LDH VI+ LHDGAPA I +HA
Sbjct: 32 MGYFGAAAAAEEVEVEVESCCGGGGGDDEAKRKLDHYVIE-------LHDGAPAEILEHA 91
Query: 61 NSNNS-SLSAAEMESLAALCDTFLPSVAAADRSAAVFFETSASTADTPERVGEMIKERVR 120
NSNN+ SLSAAEMESLAALCDTFLPSV AD SAA+FFETSA+ A TPE+VG+MIKERV+
Sbjct: 92 NSNNNCSLSAAEMESLAALCDTFLPSV-TADGSAAMFFETSAAAAGTPEKVGKMIKERVK 151
Query: 121 HPKKWLARLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFF 180
HPKKWL R+SLWMLSTWYGTYI+CGN+SLS+ FPYFQ F K+SQ +REQIVVSWSLSYFF
Sbjct: 152 HPKKWLVRVSLWMLSTWYGTYILCGNRSLSHNFPYFQTFPKLSQTKREQIVVSWSLSYFF 211
Query: 181 LLRMFFRTVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKENGRS 240
LLRM+FRT+KIFCLL FFTQVDDK NNISW AIDYPG DPQFKAKT+Q EA S
Sbjct: 212 LLRMWFRTIKIFCLLTFFTQVDDKTNNISWQAIDYPGADPQFKAKTKQLEA--------S 271
Query: 241 REVEEDEEELYGPLFKGIVNLEHQQV---AAAEALRKFGFSVTLLSPRTKASSPSMIIKC 300
REVEEDEEELYGPLFKGIV+L++QQV AAAEALRKFGF VTLLSP KASSPSMIIKC
Sbjct: 272 REVEEDEEELYGPLFKGIVHLDNQQVAAAAAAEALRKFGFPVTLLSPMIKASSPSMIIKC 331
Query: 301 DAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATD 360
DAVVVGSGSGGGVVAGVLANAGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGLVATD
Sbjct: 332 DAVVVGSGSGGGVVAGVLANAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATD 391
Query: 361 NMNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMG 420
NMNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSD+YELELFSS+LYKEAMEVVCKKMG
Sbjct: 392 NMNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDKYELELFSSNLYKEAMEVVCKKMG 451
Query: 421 VQSEIDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLV 480
VQSEIDNEGFNNAVLRKGCEELG H +NIPRNS SDHYCGWCCLGCK+GRKKGTAETWLV
Sbjct: 452 VQSEIDNEGFNNAVLRKGCEELGYHAINIPRNSTSDHYCGWCCLGCKDGRKKGTAETWLV 511
Query: 481 DLVRSGNGVILPGCEAIDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVTVVAC 540
DLVRSGNGVILPGCEAIDIR+EKKDGSERNRAKG+AFEFGCGSG KQ+ LVESKVT+VAC
Sbjct: 512 DLVRSGNGVILPGCEAIDIRKEKKDGSERNRAKGIAFEFGCGSGVKQVCLVESKVTIVAC 571
Query: 541 GALSTPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAMC 600
GALSTPPLL +SGLRNPNIGKNLHLHPVTMAWGHFPAETDK WPEE KKSYEGGIMTAMC
Sbjct: 572 GALSTPPLLLRSGLRNPNIGKNLHLHPVTMAWGHFPAETDKPWPEEHKKSYEGGIMTAMC 631
Query: 601 NIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDKG 660
NI SEPDQEPG GGAVIQTPALHPGLFSILMPW+SGTNIKQRM KFSRTAHVF LARDKG
Sbjct: 632 NIRSEPDQEPGSGGAVIQTPALHPGLFSILMPWLSGTNIKQRMRKFSRTAHVFVLARDKG 691
Query: 661 SGTIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYRE 720
SGT+KSP+ SYNM++VDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYRE
Sbjct: 692 SGTVKSPNCISYNMEEVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYRE 751
Query: 721 YEKFVKEESSRPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIADSSVF 780
YEKFVKEESSR LKDL TPICSAHQMGSCRMGI A+DSVV+QRGETWEVEGLFIADSSVF
Sbjct: 752 YEKFVKEESSRALKDLKTPICSAHQMGSCRMGIGARDSVVDQRGETWEVEGLFIADSSVF 811
Query: 781 PTALGVNPMVTVQAIAYCTAQSVLETLKRRR 796
PTALGVNPMVTVQAIAYCTAQSVLETLKR+R
Sbjct: 812 PTALGVNPMVTVQAIAYCTAQSVLETLKRKR 826
BLAST of Lcy12g013550 vs. ExPASy TrEMBL
Match:
A0A1S3BAP1 (Long-chain-alcohol oxidase OS=Cucumis melo OX=3656 GN=LOC103487628 PE=3 SV=1)
HSP 1 Score: 1349.7 bits (3492), Expect = 0.0e+00
Identity = 687/815 (84.29%), Postives = 727/815 (89.20%), Query Frame = 0
Query: 1 MGYFDATAAASCGGDGEAKRQ-LDHCVIDLHGGADRLHDGAPAGISQHANSNN------S 60
MGYF A A G DGE Q HCVI+LHGGAD HDGA ISQHANSNN
Sbjct: 12 MGYFCAAANGGGGDDGETNHQPHHHCVIELHGGADSFHDGASVEISQHANSNNDYYSSAG 71
Query: 61 SLSAAEMESLAALCDTFLPSVA---AADRSAAVFFETSASTADTPERVGEMIKERVRHPK 120
LSAAEMESLAALCDTFLPSVA AADRS AVFFETSAS TPERVGEMI ERVRHPK
Sbjct: 72 HLSAAEMESLAALCDTFLPSVAPATAADRSTAVFFETSASAVGTPERVGEMIMERVRHPK 131
Query: 121 KWLARLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFLLR 180
KWL R+SLWMLSTWYGTYI+CGN+SLSN FPYF+RFSKVSQ++REQI +SWSLSYFFLLR
Sbjct: 132 KWLVRVSLWMLSTWYGTYILCGNESLSNHFPYFKRFSKVSQKKREQIALSWSLSYFFLLR 191
Query: 181 MFFRTVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKE-NGRSRE 240
MFFRT+KI CLLAFFTQVD++MNNISW AIDYPG+DPQFK SNLKE + +SRE
Sbjct: 192 MFFRTIKILCLLAFFTQVDEEMNNISWKAIDYPGSDPQFK------PPSNLKEKDSKSRE 251
Query: 241 V-------EEDEEELYGPLFKGIVNLEHQQ--VAAAEALRKFGFSVTLLSPRTKASSPSM 300
V EE+ EELYGPLFKGIVNL+HQQ A ALRKFGF VTLLSP TK +PSM
Sbjct: 252 VDEEGDEEEEEAEELYGPLFKGIVNLQHQQQIQVAVGALRKFGFPVTLLSPTTKPPNPSM 311
Query: 301 IIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGL 360
IIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEG AMEEMY SSGL
Sbjct: 312 IIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEMYQSSGL 371
Query: 361 VATDNMNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVC 420
VATDNMNVL+LAGSTVGGGSTVNWSASI+TPSHVT+EWSDEYELELF S+LYKEA++VVC
Sbjct: 372 VATDNMNVLILAGSTVGGGSTVNWSASIKTPSHVTKEWSDEYELELFRSNLYKEAIDVVC 431
Query: 421 KKMGVQSEIDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAE 480
KKMGVQS+IDNEGFNNAVLRKGCEELG H +NIPRNS SDHYCGWCCLGC++GRKKGTAE
Sbjct: 432 KKMGVQSKIDNEGFNNAVLRKGCEELGGHAINIPRNSTSDHYCGWCCLGCRDGRKKGTAE 491
Query: 481 TWLVDLVRSGNGVILPGCEAIDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVT 540
TWLVDLVRSGNGVILPGCEAI IRQEKK G ER AKGVAFEF CGS AKQM LVESKVT
Sbjct: 492 TWLVDLVRSGNGVILPGCEAIKIRQEKKQGRERKMAKGVAFEFWCGSAAKQMCLVESKVT 551
Query: 541 VVACGALSTPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIM 600
+VACGAL+TPPLL++SGLRNPNIGKNLHLHPVTMAWG+FPAETDKSWPE+DKKSYEGGIM
Sbjct: 552 IVACGALNTPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETDKSWPEKDKKSYEGGIM 611
Query: 601 TAMCNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALA 660
TAMCNIG EP+QEPGYGGAVIQTPALHPGLFSILMPW+SGTNIKQRMCKFSRTAHVF LA
Sbjct: 612 TAMCNIGPEPNQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFVLA 671
Query: 661 RDKGSGTIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKV 720
RDKGSGT+KSPSS SY MD+VDE+NLQKGLEKALKILAAAGA EIGTHHNKGRSINVKKV
Sbjct: 672 RDKGSGTVKSPSSLSYKMDNVDEQNLQKGLEKALKILAAAGAVEIGTHHNKGRSINVKKV 731
Query: 721 SYREYEKFVKEESSRPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIAD 780
SYREYEKFVKEESSRPLKDL+TPICSAHQMGSCRMG AKDSVVNQRGETWEVEGLFIAD
Sbjct: 732 SYREYEKFVKEESSRPLKDLLTPICSAHQMGSCRMGSGAKDSVVNQRGETWEVEGLFIAD 791
Query: 781 SSVFPTALGVNPMVTVQAIAYCTAQSVLETLKRRR 796
SSVFPTALGVNPMVTVQAIAYCTAQSVLETLKR+R
Sbjct: 792 SSVFPTALGVNPMVTVQAIAYCTAQSVLETLKRKR 820
BLAST of Lcy12g013550 vs. ExPASy TrEMBL
Match:
A0A6J1CNU3 (Long-chain-alcohol oxidase OS=Momordica charantia OX=3673 GN=LOC111012719 PE=3 SV=1)
HSP 1 Score: 1324.7 bits (3427), Expect = 0.0e+00
Identity = 662/809 (81.83%), Postives = 718/809 (88.75%), Query Frame = 0
Query: 1 MGYFDATAAASCGGDGEAKRQLDHCVIDLHGGADRLHDGAPAGISQHAN-SNNSSLSAAE 60
MGY ATAAAS GGDG+ ++ HC+++LHGGAD + +G+P QHA S+N+SL+AAE
Sbjct: 1 MGYCGATAAASSGGDGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNSLTAAE 60
Query: 61 MESLAALCDTFLPSVAAAD-----------RSAAVFFETSASTADTPERVGEMIKERVRH 120
MESLAALCDTFLPSVAAA + A FF+TSAS A TPERVG MI ERVRH
Sbjct: 61 MESLAALCDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMISERVRH 120
Query: 121 PKKWLARLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFL 180
PKKW+ RLSLWMLSTWYGTYI+CGN SLSN FPYFQ FSK+S +RE+I +SWSLS+F L
Sbjct: 121 PKKWMVRLSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSLSFFLL 180
Query: 181 LRMFFRTVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKENGRSR 240
RMFFRT+KI LLAFF+QVD+KMNNISW AIDYPG DPQFK +TRQSEASNL ENGRSR
Sbjct: 181 HRMFFRTIKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNENGRSR 240
Query: 241 EVEEDEEELYGPLFKGIVNLEHQQVAAAEALRKFGFSVTLLSPRTKASSPSMIIKCDAVV 300
EEELYGPLF+GIVN++ ++ AAEALRKFGF V L PRTK +SPSM+IKCDAVV
Sbjct: 241 -----EEELYGPLFRGIVNIDQRRGVAAEALRKFGFPVAFLPPRTKTASPSMMIKCDAVV 300
Query: 301 VGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDNMNV 360
VGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGL+ATDNMNV
Sbjct: 301 VGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLLATDNMNV 360
Query: 361 LVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGVQSE 420
LVLAGSTVGGGSTVNWSAS QTP+HVTREWSD YEL+LFSS+LYKEAMEVVCKKMGVQSE
Sbjct: 361 LVLAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEVVCKKMGVQSE 420
Query: 421 IDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVDLVR 480
I++EGFNNAVLRKGCEELG H +NIPRNSP+DHYCGWCCLGCKNGRKKGTAETWLVDLVR
Sbjct: 421 IESEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGTAETWLVDLVR 480
Query: 481 SGNGVILPGCEAIDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVTVVACGALS 540
SGNG ILPGCEA+ I EKKDGSER RAKG+ FEFGC SGAKQM LVESKVT+VACGALS
Sbjct: 481 SGNGAILPGCEAVRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESKVTIVACGALS 540
Query: 541 TPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAET--DKSWPEEDKKSYEGGIMTAMCNI 600
TPPLL++SGLRNPNIGK+LHLHPVTMAWGHFPAET DK+WPEE KKSYEGGIMTAMCNI
Sbjct: 541 TPPLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYEGGIMTAMCNI 600
Query: 601 GSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDKGSG 660
G+E D EPGYGGAVIQTPALHPGLFSILMPW+SGTN K RMCKFSRTAHVFALARDKGSG
Sbjct: 601 GTEADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHVFALARDKGSG 660
Query: 661 TIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYE 720
T++S SS SY MD DEENLQKG+EKAL+ILAAAGAEEIGTHHNKGRSI VKKVSYREYE
Sbjct: 661 TVRSSSSISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIKVKKVSYREYE 720
Query: 721 KFVKEESSRPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIADSSVFPT 780
KFVKEESSRPL DL TPICSAHQMGSCRMGI KDSVVNQRGETWE+EGLFIADSSVFPT
Sbjct: 721 KFVKEESSRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGLFIADSSVFPT 780
Query: 781 ALGVNPMVTVQAIAYCTAQSVLETLKRRR 796
ALGVNPMVTVQAIAYCTAQSVL+TLKR+R
Sbjct: 781 ALGVNPMVTVQAIAYCTAQSVLDTLKRKR 804
BLAST of Lcy12g013550 vs. NCBI nr
Match:
XP_038896170.1 (long-chain-alcohol oxidase FAO4A [Benincasa hispida])
HSP 1 Score: 1389.0 bits (3594), Expect = 0.0e+00
Identity = 700/809 (86.53%), Postives = 742/809 (91.72%), Query Frame = 0
Query: 1 MGYFDATAAASCGG----DGEAKRQLDHCVIDLHGGADRLHDGAPAGISQHANSN--NSS 60
MGYFD AAS GG DG++ +Q CVI+LHGG D HDGA G SQHANS+ +S
Sbjct: 12 MGYFD--TAASGGGSDIDDGQSNQQCQQCVIELHGGTDSFHDGASVGFSQHANSDDLSSH 71
Query: 61 LSAAEMESLAALCDTFLPSV---AAADRSAAVFFETSASTADTPERVGEMIKERVRHPKK 120
LSAAEMESLAALCDTFLPSV AAADRS +FFETSAS A TPERVGEMIKERVRHPKK
Sbjct: 72 LSAAEMESLAALCDTFLPSVAATAAADRSVTLFFETSASAAGTPERVGEMIKERVRHPKK 131
Query: 121 WLARLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFLLRM 180
WL R++LWMLSTWYGTYI+CGN+SLSN FPYF+RFSKVSQ++REQI +SWSLSYFFLLRM
Sbjct: 132 WLVRMTLWMLSTWYGTYILCGNESLSNHFPYFERFSKVSQKKREQIAISWSLSYFFLLRM 191
Query: 181 FFRTVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKENG-RSREV 240
FFRT+KI CLLAFFTQVD++ NNISW AIDYPG+DPQFKAKT+ SEASNLKE G +SRE+
Sbjct: 192 FFRTIKILCLLAFFTQVDEERNNISWKAIDYPGSDPQFKAKTKHSEASNLKEKGSKSREI 251
Query: 241 --EEDEEELYGPLFKGIVNLEHQQ--VAAAEALRKFGFSVTLLSPRTKASSPSMIIKCDA 300
E+DEEELYGPLFKGIVNL+HQQ A ALRKFGF VTLLS TK +PSMIIKCDA
Sbjct: 252 DQEDDEEELYGPLFKGIVNLQHQQQVQVAVGALRKFGFPVTLLSLSTKPPNPSMIIKCDA 311
Query: 301 VVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDNM 360
VVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEG AMEEMY +SGLVATDNM
Sbjct: 312 VVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEMYQASGLVATDNM 371
Query: 361 NVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGVQ 420
NVL+LAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELF S+LYKEAMEVVCKKMGVQ
Sbjct: 372 NVLILAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKEAMEVVCKKMGVQ 431
Query: 421 SEIDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVDL 480
SEIDNEGFNNAVLRKGCEELG H MNIPRNS SDHYCGWCCLGCKNGRKKGTAETWLVDL
Sbjct: 432 SEIDNEGFNNAVLRKGCEELGCHAMNIPRNSTSDHYCGWCCLGCKNGRKKGTAETWLVDL 491
Query: 481 VRSGNGVILPGCEAIDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVTVVACGA 540
VRSGNG ILPGCEAI IRQEK+DGS+ NRAKG+ FEFGCGS AKQM LVESKVT+VACGA
Sbjct: 492 VRSGNGAILPGCEAIKIRQEKRDGSKCNRAKGIVFEFGCGSAAKQMCLVESKVTIVACGA 551
Query: 541 LSTPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAMCNI 600
L+TPPLL++SGLRNPNIGKNLHLHPVTMAWG+FPAETDKSWPE+DKKSYEGGIMTAMCNI
Sbjct: 552 LNTPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETDKSWPEKDKKSYEGGIMTAMCNI 611
Query: 601 GSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDKGSG 660
GSEPDQEPGYGGAVIQTPALHPGLFSILMPW+SGTNIK RMCKFSRTAHVFALARDKGSG
Sbjct: 612 GSEPDQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKHRMCKFSRTAHVFALARDKGSG 671
Query: 661 TIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYE 720
T+KS SS SY MDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYE
Sbjct: 672 TVKSSSSLSYKMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYE 731
Query: 721 KFVKEESSRPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIADSSVFPT 780
KFVKEESSRPLKDL+TPICSAHQMGSCRMGI AKDSVVNQRGETWEVEGLFIADSSVFPT
Sbjct: 732 KFVKEESSRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPT 791
Query: 781 ALGVNPMVTVQAIAYCTAQSVLETLKRRR 796
ALGVNPMVTVQAIAYCTAQS+LETLKR+R
Sbjct: 792 ALGVNPMVTVQAIAYCTAQSILETLKRKR 818
BLAST of Lcy12g013550 vs. NCBI nr
Match:
XP_022937010.1 (long-chain-alcohol oxidase FAO4A-like [Cucurbita moschata])
HSP 1 Score: 1387.9 bits (3591), Expect = 0.0e+00
Identity = 699/795 (87.92%), Postives = 737/795 (92.70%), Query Frame = 0
Query: 1 MGYFDATAAASCGGDGEAKRQLDHCVIDLHGGADRLHDGAPAGISQHANSNNSSLSAAEM 60
MGYF AAASCGGDGE+K QL CVI+L+GGAD LHDG PAGISQHAN N SLSAAEM
Sbjct: 12 MGYFG--AAASCGGDGESKPQLHQCVIELNGGADSLHDGGPAGISQHANF-NYSLSAAEM 71
Query: 61 ESLAALCDTFLPSVAAADRSAAVFFETSASTADTPERVGEMIKERVRHPKKWLARLSLWM 120
ESLAALCDTFLPSVAA D SA+ FFETSAS A TPERVG MIKERVRHPKKWL R+SLWM
Sbjct: 72 ESLAALCDTFLPSVAATDGSASEFFETSASAAGTPERVGGMIKERVRHPKKWLMRVSLWM 131
Query: 121 LSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFLLRMFFRTVKIFC 180
LSTWYGTYI+CGN SLSN FPYFQRFSKVSQ++REQ+VVSWSLSYF LLRMFFRT+KI C
Sbjct: 132 LSTWYGTYILCGNGSLSNHFPYFQRFSKVSQKKREQVVVSWSLSYFLLLRMFFRTIKILC 191
Query: 181 LLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKENGRSREVEEDEEELYGP 240
LAFFTQVD++MNNISW AIDYPG+DPQFKAK RQSE NLKE RSRE+ E+EEELYGP
Sbjct: 192 HLAFFTQVDEEMNNISWKAIDYPGSDPQFKAKARQSEEWNLKEKVRSREIVEEEEELYGP 251
Query: 241 LFKGIVNLEHQQVAAAEALRKFGFSVTLLSPRTKASSPSMIIKCDAVVVGSGSGGGVVAG 300
LFKGIVNLEHQQVA ALRKFGF VTLLSP K+ SPSMIIKCDAVVVGSGSGGGVVAG
Sbjct: 252 LFKGIVNLEHQQVAVG-ALRKFGFPVTLLSPSIKSPSPSMIIKCDAVVVGSGSGGGVVAG 311
Query: 301 VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDNMNVLVLAGSTVGGGS 360
VLA+AGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGLVATDNMNVL+LAGSTVGGGS
Sbjct: 312 VLASAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNVLILAGSTVGGGS 371
Query: 361 TVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGVQSEIDNEGFNNAVLR 420
TVNWSASIQTPSHVTREWSDEYELELF S+LYKEA+EVVCK+MGVQSEIDNEGFNNAVLR
Sbjct: 372 TVNWSASIQTPSHVTREWSDEYELELFGSNLYKEALEVVCKRMGVQSEIDNEGFNNAVLR 431
Query: 421 KGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGVILPGCEA 480
KGCEELG +NIPRNS SDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNG ILPGCEA
Sbjct: 432 KGCEELGCKAINIPRNSASDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA 491
Query: 481 IDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVTVVACGALSTPPLLRKSGLRN 540
I I QE KDG +RNRAKGVAFEFGCGSGAKQM LVESKVT+VACGAL TP LL++SGLRN
Sbjct: 492 IRITQE-KDGRKRNRAKGVAFEFGCGSGAKQMCLVESKVTIVACGALCTPQLLKRSGLRN 551
Query: 541 PNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAMCNIGSEPDQEPGYGGAV 600
PNIGKNLHLHPVTMAWG+FPAETD+SWPE+DKKSYEGGIMTAMCNIGS PDQEPGYGGAV
Sbjct: 552 PNIGKNLHLHPVTMAWGYFPAETDESWPEKDKKSYEGGIMTAMCNIGSSPDQEPGYGGAV 611
Query: 601 IQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDKGSGTIKSPSSFSYNMDD 660
IQTPALHPGLFSILMPW+SGTNIKQRMCKFSRTAHVFALARDKGSGT++S SS SY MDD
Sbjct: 612 IQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFALARDKGSGTVESSSSLSYIMDD 671
Query: 661 VDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYEKFVKEESSRPLKDL 720
DE+NLQKGLEKALKIL AAGAEEIGTHHNKGRSINVKKVSYREYE+FVKEESSRPLKDL
Sbjct: 672 GDEKNLQKGLEKALKILGAAGAEEIGTHHNKGRSINVKKVSYREYERFVKEESSRPLKDL 731
Query: 721 MTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA 780
+TPICSAHQMGSCRMGI AKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA
Sbjct: 732 LTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA 791
Query: 781 YCTAQSVLETLKRRR 796
YCTA+SVLETLKR+R
Sbjct: 792 YCTAKSVLETLKRKR 801
BLAST of Lcy12g013550 vs. NCBI nr
Match:
XP_023535226.1 (long-chain-alcohol oxidase FAO4A-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1385.2 bits (3584), Expect = 0.0e+00
Identity = 702/795 (88.30%), Postives = 737/795 (92.70%), Query Frame = 0
Query: 1 MGYFDATAAASCGGDGEAKRQLDHCVIDLHGGADRLHDGAPAGISQHANSNNSSLSAAEM 60
MGYF AAAS GG+GE+K QL CVIDLHGGAD +DG PAGISQHANS N SLSAAEM
Sbjct: 12 MGYFG--AAASYGGNGESKPQLHQCVIDLHGGAD--NDGGPAGISQHANS-NYSLSAAEM 71
Query: 61 ESLAALCDTFLPSVAAADRSAAVFFETSASTADTPERVGEMIKERVRHPKKWLARLSLWM 120
ESLAALCDTFLPSVAA D SA+ +FETSAS A TPERVG MIKERVRHPKKWL R+SLWM
Sbjct: 72 ESLAALCDTFLPSVAATDGSASEYFETSASAAGTPERVGGMIKERVRHPKKWLMRVSLWM 131
Query: 121 LSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFLLRMFFRTVKIFC 180
LSTWYGTYI+CGN SLSN FPYFQRFSKVSQR REQ+VVSWSLSYF LLRMF+RTVKI C
Sbjct: 132 LSTWYGTYILCGNGSLSNHFPYFQRFSKVSQREREQVVVSWSLSYFLLLRMFYRTVKILC 191
Query: 181 LLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKENGRSREVEEDEEELYGP 240
LAFFTQVD++MNNISW AIDYPG+DPQFKAK RQSE NLKE RSRE+EE+EEELYGP
Sbjct: 192 HLAFFTQVDEEMNNISWKAIDYPGSDPQFKAKARQSEEWNLKEKVRSREIEEEEEELYGP 251
Query: 241 LFKGIVNLEHQQVAAAEALRKFGFSVTLLSPRTKASSPSMIIKCDAVVVGSGSGGGVVAG 300
LFKGIVNLEHQQVA ALRKFGF VTLLSP TK+ SPSMIIKCDAVVVGSGSGGGVVAG
Sbjct: 252 LFKGIVNLEHQQVAVG-ALRKFGFPVTLLSPSTKSPSPSMIIKCDAVVVGSGSGGGVVAG 311
Query: 301 VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDNMNVLVLAGSTVGGGS 360
VLA+AGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGLVATDNMNVL+LAGSTVGGGS
Sbjct: 312 VLASAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNVLILAGSTVGGGS 371
Query: 361 TVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGVQSEIDNEGFNNAVLR 420
TVNWSASIQTPSHVTREWSDEYELELF S+LYKEA+EVVCK+MGVQSEI+NEGFNNAVLR
Sbjct: 372 TVNWSASIQTPSHVTREWSDEYELELFRSNLYKEALEVVCKRMGVQSEIENEGFNNAVLR 431
Query: 421 KGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGVILPGCEA 480
KGCEELG MNIPRNS SDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNG ILPGCEA
Sbjct: 432 KGCEELGCKAMNIPRNSASDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA 491
Query: 481 IDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVTVVACGALSTPPLLRKSGLRN 540
I I QE KDG ERNRAKGVAFEF CGSGAKQM LVESKVT+VACGAL TPPLL++SGLRN
Sbjct: 492 IRITQE-KDGRERNRAKGVAFEFECGSGAKQMCLVESKVTIVACGALCTPPLLKRSGLRN 551
Query: 541 PNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAMCNIGSEPDQEPGYGGAV 600
PNIGKNLHLHPVTMAWG+FPAETD+SWPE+DKKSYEGGIMTAMCNIGS PDQEPGYGGAV
Sbjct: 552 PNIGKNLHLHPVTMAWGYFPAETDESWPEKDKKSYEGGIMTAMCNIGSSPDQEPGYGGAV 611
Query: 601 IQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDKGSGTIKSPSSFSYNMDD 660
IQTPALHPGLFSILMPW+SGTNIKQRMCKFSRTAHVFALARDKGSGT++S SS SY MDD
Sbjct: 612 IQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFALARDKGSGTVESSSSLSYIMDD 671
Query: 661 VDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYEKFVKEESSRPLKDL 720
DE+NLQKGLEKALKIL AAGAEEIGTHHNKGRSINVKKVSYREYE+FVKEESSRPLKDL
Sbjct: 672 GDEKNLQKGLEKALKILGAAGAEEIGTHHNKGRSINVKKVSYREYERFVKEESSRPLKDL 731
Query: 721 MTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA 780
+TPICSAHQMGSCRMGI AKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA
Sbjct: 732 LTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA 791
Query: 781 YCTAQSVLETLKRRR 796
YCTAQSVLETLKR+R
Sbjct: 792 YCTAQSVLETLKRKR 799
BLAST of Lcy12g013550 vs. NCBI nr
Match:
KAG6591858.1 (Long-chain-alcohol oxidase FAO4A, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1380.2 bits (3571), Expect = 0.0e+00
Identity = 694/795 (87.30%), Postives = 735/795 (92.45%), Query Frame = 0
Query: 1 MGYFDATAAASCGGDGEAKRQLDHCVIDLHGGADRLHDGAPAGISQHANSNNSSLSAAEM 60
MGYF AT AS GGDGE+K QL CVI+L+GGAD +DG PAGISQHANS N SLSAAEM
Sbjct: 12 MGYFGAT--ASYGGDGESKPQLHQCVIELNGGADSRYDGGPAGISQHANS-NYSLSAAEM 71
Query: 61 ESLAALCDTFLPSVAAADRSAAVFFETSASTADTPERVGEMIKERVRHPKKWLARLSLWM 120
ESLAALCDTFLPSVAA D SA+ FFETSAS A TPERVG MIKERVRHPKKWL R+SLWM
Sbjct: 72 ESLAALCDTFLPSVAATDGSASEFFETSASAAGTPERVGGMIKERVRHPKKWLMRVSLWM 131
Query: 121 LSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFLLRMFFRTVKIFC 180
LSTWYGTYI+CGN SLSN FPYFQRFSKVSQ++REQ+VVSWSLSYF LL+MFFRT+KI C
Sbjct: 132 LSTWYGTYILCGNGSLSNHFPYFQRFSKVSQKKREQVVVSWSLSYFLLLKMFFRTIKILC 191
Query: 181 LLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKENGRSREVEEDEEELYGP 240
LAFFTQVD++MNNISW AIDYPG+DPQFKAK RQSE NLKE RSRE+EE+EEELYGP
Sbjct: 192 HLAFFTQVDEEMNNISWKAIDYPGSDPQFKAKARQSEEWNLKEKVRSREIEEEEEELYGP 251
Query: 241 LFKGIVNLEHQQVAAAEALRKFGFSVTLLSPRTKASSPSMIIKCDAVVVGSGSGGGVVAG 300
LFKGIVNLEHQQVA ALRKFGF VTLLSP K+ SPSMIIKCDAVVVGSGSGGGVVAG
Sbjct: 252 LFKGIVNLEHQQVAVG-ALRKFGFPVTLLSPSIKSPSPSMIIKCDAVVVGSGSGGGVVAG 311
Query: 301 VLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDNMNVLVLAGSTVGGGS 360
VLA+AGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGLVATDNMNVL+LAGSTVGGGS
Sbjct: 312 VLASAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNVLILAGSTVGGGS 371
Query: 361 TVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGVQSEIDNEGFNNAVLR 420
TVNWSASIQTPSHVTREWS EYELELF S+LYKEA+EVVCK+MGVQSE+DNEGFNNA+LR
Sbjct: 372 TVNWSASIQTPSHVTREWSGEYELELFGSNLYKEALEVVCKRMGVQSEMDNEGFNNAILR 431
Query: 421 KGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGVILPGCEA 480
KGCEELG MNIPRNS SDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNG ILPGCEA
Sbjct: 432 KGCEELGCKAMNIPRNSASDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGNGAILPGCEA 491
Query: 481 IDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVTVVACGALSTPPLLRKSGLRN 540
I I QE KDG +RNRAKGVAFEFGCGSGAKQM LVESKVT+VACGAL TPPLL++SGL N
Sbjct: 492 IRITQE-KDGRKRNRAKGVAFEFGCGSGAKQMCLVESKVTIVACGALCTPPLLKRSGLTN 551
Query: 541 PNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAMCNIGSEPDQEPGYGGAV 600
PNIGKNLHLHPV MAWG+FPAETD+SWPE+DKKSYEGGIMTAMCNIGS PDQEPGYGGAV
Sbjct: 552 PNIGKNLHLHPVAMAWGYFPAETDESWPEKDKKSYEGGIMTAMCNIGSSPDQEPGYGGAV 611
Query: 601 IQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDKGSGTIKSPSSFSYNMDD 660
IQTPALHPGLFSILMPW+SGTNIKQ+MCKFSRTAHVFALARDKGSGT++S SS SY MDD
Sbjct: 612 IQTPALHPGLFSILMPWVSGTNIKQKMCKFSRTAHVFALARDKGSGTVESSSSLSYIMDD 671
Query: 661 VDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYEKFVKEESSRPLKDL 720
DE+NLQKGLEKALKIL AAGAEEIGTHHNKGRSINVKKVSYREYE+FVKEESSRPLKDL
Sbjct: 672 GDEKNLQKGLEKALKILGAAGAEEIGTHHNKGRSINVKKVSYREYERFVKEESSRPLKDL 731
Query: 721 MTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA 780
+TPICSAHQMGSCRMGI AKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA
Sbjct: 732 LTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIA 791
Query: 781 YCTAQSVLETLKRRR 796
YCTAQSVLETLKR+R
Sbjct: 792 YCTAQSVLETLKRKR 801
BLAST of Lcy12g013550 vs. NCBI nr
Match:
XP_023523874.1 (long-chain-alcohol oxidase FAO4A-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1377.8 bits (3565), Expect = 0.0e+00
Identity = 703/809 (86.90%), Postives = 741/809 (91.59%), Query Frame = 0
Query: 1 MGYFDATAAA-------SC---GGDGEAKRQLDHCVIDLHGGADRLHDGAPAGISQHANS 60
MGYF A AAA SC GGD EAKR+LDH VI+ LHDGAPA I +HANS
Sbjct: 1 MGYFGAAAAAAEEVEVESCGGGGGDDEAKRKLDHYVIE-------LHDGAPAEILEHANS 60
Query: 61 NNS-SLSAAEMESLAALCDTFLPSVA-AADRSAAVFFETSASTADTPERVGEMIKERVRH 120
NN+ SLSAAEMESLAALCDTFLPSV AD SAA+FFETSAS A TPE+VG+MIKERV+H
Sbjct: 61 NNNCSLSAAEMESLAALCDTFLPSVVNTADGSAAMFFETSASAAGTPEKVGKMIKERVKH 120
Query: 121 PKKWLARLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFL 180
PKKWL R+SLWMLSTWYGTYI+CGN+SLS+ FPYFQ F K+SQ +REQIVVSWSLSYFFL
Sbjct: 121 PKKWLVRVSLWMLSTWYGTYILCGNRSLSHHFPYFQTFPKLSQTKREQIVVSWSLSYFFL 180
Query: 181 LRMFFRTVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKENGRSR 240
LRM+FRT+KIFCLL FFTQVDDK NNISW AIDYPG DPQFKAKT+Q EASN KEN RSR
Sbjct: 181 LRMWFRTIKIFCLLTFFTQVDDKTNNISWQAIDYPGADPQFKAKTKQLEASNQKENDRSR 240
Query: 241 EVEEDEEELYGPLFKGIVNLEHQQ--VAAAEALRKFGFSVTLLSPRTKASSPSMIIKCDA 300
EVEED+EELYGPLFKGIVNL++QQ AAAEALRKFGF VTLLSP KASSPSMIIKCDA
Sbjct: 241 EVEEDKEELYGPLFKGIVNLDNQQAAAAAAEALRKFGFPVTLLSPMIKASSPSMIIKCDA 300
Query: 301 VVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDNM 360
VVVGSGSGGGVVAGVLANAGYKV+VLEKGSYCARTNLSLLEG AMEEMYLSSGLVATDNM
Sbjct: 301 VVVGSGSGGGVVAGVLANAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNM 360
Query: 361 NVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGVQ 420
NVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSS+LYKEAMEVVCKKMGVQ
Sbjct: 361 NVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSNLYKEAMEVVCKKMGVQ 420
Query: 421 SEIDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVDL 480
SEI NEGFNNAVLRKGCEELG H +NIPRNS SDHYCGWCCLGCK+GRKKGTAETWLVDL
Sbjct: 421 SEIHNEGFNNAVLRKGCEELGYHAINIPRNSTSDHYCGWCCLGCKDGRKKGTAETWLVDL 480
Query: 481 VRSGNGVILPGCEAIDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVTVVACGA 540
VRSGNGVILPGCEAI+IR+EK DGSERNRAKGVAFEFGCGSG KQ+ LVESKVT+VACGA
Sbjct: 481 VRSGNGVILPGCEAINIRKEKNDGSERNRAKGVAFEFGCGSGVKQVCLVESKVTIVACGA 540
Query: 541 LSTPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAMCNI 600
LSTPPLL +SGLRNPNIGKNLHLHPVTMAWGHFPAETDK WPEE KKSYEGGIMTAMCNI
Sbjct: 541 LSTPPLLLRSGLRNPNIGKNLHLHPVTMAWGHFPAETDKPWPEEHKKSYEGGIMTAMCNI 600
Query: 601 GSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDKGSG 660
SEPDQEPG GGAVIQTPALHPGLFSILMPW+SG+NIKQRM KFSRTAHVF LARDKGSG
Sbjct: 601 RSEPDQEPGSGGAVIQTPALHPGLFSILMPWLSGSNIKQRMRKFSRTAHVFVLARDKGSG 660
Query: 661 TIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYE 720
T+KSP+ SYNM++VDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYE
Sbjct: 661 TVKSPNCISYNMEEVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYE 720
Query: 721 KFVKEESSRPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIADSSVFPT 780
KFVKEESSR LKDL TPICSAHQMGSCRMGI A+DSVV+QRGETWEVEGLFIADSSVFPT
Sbjct: 721 KFVKEESSRALKDLKTPICSAHQMGSCRMGIGARDSVVDQRGETWEVEGLFIADSSVFPT 780
Query: 781 ALGVNPMVTVQAIAYCTAQSVLETLKRRR 796
ALGVNPMVTVQAIAYCTAQSVLETLKR+R
Sbjct: 781 ALGVNPMVTVQAIAYCTAQSVLETLKRKR 802
BLAST of Lcy12g013550 vs. TAIR 10
Match:
AT4G19380.1 (Long-chain fatty alcohol dehydrogenase family protein )
HSP 1 Score: 866.3 bits (2237), Expect = 1.9e-251
Identity = 438/744 (58.87%), Postives = 560/744 (75.27%), Query Frame = 0
Query: 60 MESLAALCDTFLPS-----VAAADRSAAVFFETSASTADTPERVGEMIKERVRHPKKWLA 119
MESL A+CDTF+ S V D A +F SAS TP+RV ++ ER+ HPKKW+
Sbjct: 1 MESLVAICDTFISSIDDSGVGHVDDCVAGYFSASASQTGTPDRVARLMSERLHHPKKWIL 60
Query: 120 RLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFLLRMFFR 179
R LW+LSTW G+ ++CG +S + +FPYF+RF ++ ++RRE+I+++WS SYF LLRM FR
Sbjct: 61 RAGLWLLSTWIGSLVLCGWRSFTGEFPYFRRFCRLPEKRREEILLNWSSSYFSLLRMLFR 120
Query: 180 TVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASNLKENGRSREVEEDE 239
T+K+ L FFTQVD+K N++W AI Y G P L E + ++
Sbjct: 121 TIKLISALVFFTQVDEKGRNLAWKAIGYNGPSPD-----HSDHEVELNEEKKKKK----P 180
Query: 240 EELYGPLFKGIVNLEHQQVAAAEALRKFGFSVTLLSPRTKASS---PSMIIKCDAVVVGS 299
EE++GPL+ GIV+L+ + A + L GF+V+ T SS P M I+CDAVVVGS
Sbjct: 181 EEIFGPLYNGIVDLKSPREAVEKKLAGRGFAVSNQKRNTNGSSISDPVMKIQCDAVVVGS 240
Query: 300 GSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDNMNVLVL 359
GSGGGV AGVLA AGYKVLV+E G+Y AR+ LSLLEGQAM++MYLS GL+AT + NV++L
Sbjct: 241 GSGGGVAAGVLAKAGYKVLVIESGNYYARSKLSLLEGQAMDDMYLSGGLLATSDTNVVIL 300
Query: 360 AGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGVQSEIDN 419
AGSTVGGGST+NWSASI+TP HV +EW+++ +LE+F S LY+EAM+VVCK+MGVQ
Sbjct: 301 AGSTVGGGSTINWSASIKTPEHVMKEWAEKSKLEMFGSDLYREAMDVVCKRMGVQCGFVE 360
Query: 420 EGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVDLVRSGN 479
EGFNN VLRKGCE+LG V NIPRN+PSDHYCG+CCLGCK G+K+GT+ETWLVDLV S N
Sbjct: 361 EGFNNEVLRKGCEKLGLPVKNIPRNAPSDHYCGFCCLGCKKGQKQGTSETWLVDLVESDN 420
Query: 480 GVILPGCEAIDIRQEKKDGSERNRAKGVAFEFGCGSGAKQMFLVESKVTVVACGALSTPP 539
G+ILPGC+A ++ + + G ++ +A GVAF FG +++++VES+VT+VACGAL TP
Sbjct: 421 GLILPGCQATEVMYDCEQG-KKKKATGVAFAFG-----EEIYVVESRVTIVACGALRTPH 480
Query: 540 LLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAMCNIGSEPD 599
LL++SGL+N NIG+NL LHPV MAWG FP E DK WPE+ KKSYEGGIMTAM ++ E +
Sbjct: 481 LLKRSGLKNSNIGRNLCLHPVVMAWGWFP-EEDK-WPEKKKKSYEGGIMTAMSSVVIE-E 540
Query: 600 QEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDKGSGTIKSP 659
YG VIQTPALHPG+FS ++PW S + K RM KFSRTAH+FAL RDKG+GTI S
Sbjct: 541 THSSYGEMVIQTPALHPGMFSGIIPWTSSKDFKTRMLKFSRTAHIFALLRDKGTGTIDSK 600
Query: 660 SSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYEKFVKE 719
+ YN++D DEE+L+ GLE+ LKILAAAGAEEIGTHH++GRS+NV+ S E E+FV+E
Sbjct: 601 TYIDYNLNDEDEESLKNGLERVLKILAAAGAEEIGTHHSEGRSLNVRTASSLEIERFVRE 660
Query: 720 ESSRPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIADSSVFPTALGVN 779
ESS+PLKDL ICSAHQMGSCRMGIR ++S V GETWEVE LF+AD+SVFPTALGVN
Sbjct: 661 ESSKPLKDLSGQICSAHQMGSCRMGIRPEESAVRPTGETWEVERLFVADTSVFPTALGVN 720
Query: 780 PMVTVQAIAYCTAQSVLETLKRRR 796
PMVTVQ+IAYC +V++ LK+++
Sbjct: 721 PMVTVQSIAYCIGLNVVDVLKKKK 726
BLAST of Lcy12g013550 vs. TAIR 10
Match:
AT4G28570.1 (Long-chain fatty alcohol dehydrogenase family protein )
HSP 1 Score: 525.0 bits (1351), Expect = 1.0e-148
Identity = 305/755 (40.40%), Postives = 441/755 (58.41%), Query Frame = 0
Query: 52 NSSLSAAEMESLAALCDTFLPSVAAADRSAAVFFETSASTADT-PERVGEMIKERVRHPK 111
N S ++++SLA +C TFLP +++ A F ++ST + V EMI + R
Sbjct: 24 NHGFSPSQIQSLAVICQTFLPPETTSEQQAVNSFHVASSTQPPFTDEVAEMIVKNGRSEA 83
Query: 112 KWLARLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSWSLSYFFLL- 171
+ R+ L +LS +GT ++CG+ L +P+ +FS++ +RE I+ +WS FLL
Sbjct: 84 VKVLRIILMILSFRFGTLLLCGSLCLDKSWPFVLKFSQLPLDKREAILRNWSRQSGFLLP 143
Query: 172 -RMFFRTVKIFCLLAFFTQVDDKMNNISWNAIDY--PGTDPQFKAKTRQSEASNLKENGR 231
R+ F K + L FF+Q D+ + N + AI Y GT+ R
Sbjct: 144 FRITFFLAKFYTLFYFFSQTDENLKNPALEAIGYCIDGTE-------------------R 203
Query: 232 SREVEEDEEELYGPLFKGIVNLEHQ-QVAAAEALRKFGFSVTLLSPRTKASSPSMIIKCD 291
S + + +E PL KGI+ H+ V ++L + G V I+CD
Sbjct: 204 SSNKKSEADEKRRPLEKGIIETMHESDVTITQSLTEKGVHVA-----RDDGDNVYRIRCD 263
Query: 292 AVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSGLVATDN 351
AVVVGSGSGGGV A LA AG KVLVLEKG+Y + S LE +M E+Y GL+ T +
Sbjct: 264 AVVVGSGSGGGVAAANLAKAGLKVLVLEKGNYFTAHDYSGLEVPSMLELYEKGGLLTTVD 323
Query: 352 MNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVVCKKMGV 411
++LAGS VGGG+ VNWSASI+TP HV +EWS+ +++ F S Y+ AM+ V ++GV
Sbjct: 324 GKFMLLAGSAVGGGTAVNWSASIRTPDHVLQEWSEGSKIKFFGSQEYQSAMDEVTIRIGV 383
Query: 412 QSEIDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTAETWLVD 471
GF N VLRKGCE LG V ++PRNSP DHYCG C GC+ G K GT +TWLVD
Sbjct: 384 TERCVKHGFQNQVLRKGCERLGLQVESVPRNSPEDHYCGLCGYGCRAGAKNGTDQTWLVD 443
Query: 472 LVRSGNGVILPGCEAIDIRQEKKDGSERNRAKGVAFEFGCGSGAK--QMFLVESKVTVVA 531
V +G VIL G +A S R K F G K + F++E++VTV +
Sbjct: 444 AVENG-AVILTGIKAERFVLVDNTSSSNERKKRCVGVFASSVGGKIGKKFIIEARVTVSS 503
Query: 532 CGALSTPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYEGGIMTAM 591
G+L TPPL+ SGL+NPNIG+NL LHPV M WG+FP E D E K YEGGI+T++
Sbjct: 504 AGSLLTPPLMLSSGLKNPNIGRNLKLHPVLMTWGYFP-EKDS---EFSGKMYEGGIITSV 563
Query: 592 CNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHVFALARDK 651
++ D E G A+++ P + P ++ L PW+SG ++K+RM K+ RTAH+FAL RD
Sbjct: 564 HHMN---DTESGC-KAILENPLIGPASYAGLSPWVSGPDLKERMIKYGRTAHLFALVRDL 623
Query: 652 GSGTIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYR 711
GSG + + +Y D ENL+ GL +AL++ AAGA E+GT+ + G+ + + ++
Sbjct: 624 GSGEVMMENEVTYRTTKKDRENLRAGLRQALRVSVAAGAVEVGTYRSDGQKMKCEAITKE 683
Query: 712 EYEKFVKEESS----RPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWEVEGLFIA 771
E+F+ E + + T SAHQMGSCRMG+ A++ +++ GE+WE EGLF+
Sbjct: 684 AMEEFLDEVDAVGGVGTKGEYWTTYFSAHQMGSCRMGVTAEEGALDENGESWEAEGLFVC 743
Query: 772 DSSVFPTALGVNPMVTVQAIAYCTAQSVLETLKRR 795
D S+ P+A+GVNPM+T+Q+ AYC + ++++L+ +
Sbjct: 744 DGSILPSAVGVNPMITIQSTAYCISSKIVDSLQNK 745
BLAST of Lcy12g013550 vs. TAIR 10
Match:
AT3G23410.1 (fatty alcohol oxidase 3 )
HSP 1 Score: 522.7 bits (1345), Expect = 5.2e-148
Identity = 307/759 (40.45%), Postives = 447/759 (58.89%), Query Frame = 0
Query: 55 LSAAEMESLAALCDTFLPSVAAADRSAA-------------VFFETSASTADTPERVGEM 114
++ AEMESLA+ C+ LPSV + F+ TS S + E+
Sbjct: 15 ITVAEMESLASFCEAVLPSVQPPPEELSGEGDNHRNKEALRSFYSTSGSKTPVLRQSIEL 74
Query: 115 IKERVRHPKKWLARLSLWMLSTWYGTYIMCGNKSLSNKFPYFQRFSKVSQRRREQIVVSW 174
+ +R RL L++L+T GT ++CG + L +++P+ ++FS++S +RE+++
Sbjct: 75 VTKRGTIEAYIATRLILFLLATRLGTLLICGTECLVSRWPFVEKFSELSLEKRERVLQKQ 134
Query: 175 SLSYFFL-LRMFFRTVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTRQSEASN 234
++ +R F +K+ L FF++V+ N +W AI Y +P + + SE N
Sbjct: 135 FKNWILTPIRAAFVYIKVAFLFCFFSRVNPNGENPAWEAIGY-RVNPD---ENKPSETHN 194
Query: 235 LKENGRSREVEEDEEELYGPLFKGIV-NLEHQQVAAAEALRKFGFSVTLLSPRTKASSPS 294
+ PL KGIV +E + E+L G L +
Sbjct: 195 ER-----------------PLEKGIVETMEETEQTLLESLAHKGLEAVL-----DTEHDA 254
Query: 295 MIIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSSG 354
+ IKCD VVVGSGSGGGV A VLA +G KV+VLEKGSY + EG ++++Y + G
Sbjct: 255 IRIKCDVVVVGSGSGGGVAASVLAKSGLKVVVLEKGSYFTPSEHRPFEGPGLDKLYENGG 314
Query: 355 LVATDNMNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYKEAMEVV 414
++ + + + +VLAG+TVGGGS VNWSA I+TP V +EWS++ + LF + Y AMEVV
Sbjct: 315 ILPSVDGSFMVLAGATVGGGSAVNWSACIKTPKSVLQEWSEDQNIPLFGTKEYLTAMEVV 374
Query: 415 CKKMGVQSEIDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNGRKKGTA 474
K+MGV + + E F N +LRKGCE LG +V N+PRNS HYCG C GC+ G KKG+
Sbjct: 375 WKRMGVTEKCELESFQNQILRKGCENLGFNVENVPRNSSESHYCGSCGYGCRQGDKKGSD 434
Query: 475 ETWLVDLVRSGNGVILPGCEAIDIRQEK---KDGSERNRAKGVAFEFGCGSGAKQMFLVE 534
TWLVD V G VIL GC+A EK G ++ + GV + G+ AK M +E
Sbjct: 435 RTWLVDAVGHG-AVILTGCKAERFILEKNGSNKGGKQMKCLGVMAKSLNGNIAK-MLKIE 494
Query: 535 SKVTVVACGALSTPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSWPEEDKKSYE 594
+KVTV A GAL TPPL+ SGLRN NIGKNLHLHPV MAWG+FP + + S SYE
Sbjct: 495 AKVTVSAGGALLTPPLMISSGLRNRNIGKNLHLHPVLMAWGYFP-DKESSNISFKGNSYE 554
Query: 595 GGIMTAMCNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRMCKFSRTAHV 654
GGI+T++ + SE + A+I+TP L PG FS+L PW SG ++K+RM ++SRTA +
Sbjct: 555 GGIITSVSKVLSEDSEV----RAIIETPQLGPGSFSVLTPWTSGLDMKKRMARYSRTASL 614
Query: 655 FALARDKGSGTIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSIN 714
+ RD+GSG +K+ +Y +D D +NL+ GL ++L+IL AAGAEE+GTH + G+ +
Sbjct: 615 ITIVRDRGSGEVKTEGRINYTVDKTDRDNLKAGLRESLRILIAAGAEEVGTHRSDGQRLI 674
Query: 715 VKKVSYREYEKFV----KEESSRPLKDLMTPICSAHQMGSCRMGIRAKDSVVNQRGETWE 774
K V+ ++F+ EE ++ + + SAHQMGSCR+G K+ ++ GE+WE
Sbjct: 675 CKGVNENSIQEFLDSVSTEEGAKGMTEKWNVYSSAHQMGSCRIGENEKEGAIDLNGESWE 734
Query: 775 VEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSVLETL 792
E LF+ D+S P+A+GVNPM+TV + AYC + + +++
Sbjct: 735 AEKLFVCDASALPSAVGVNPMITVMSTAYCISTRIAKSM 740
BLAST of Lcy12g013550 vs. TAIR 10
Match:
AT1G03990.1 (Long-chain fatty alcohol dehydrogenase family protein )
HSP 1 Score: 496.5 bits (1277), Expect = 4.0e-140
Identity = 286/772 (37.05%), Postives = 446/772 (57.77%), Query Frame = 0
Query: 50 SNNSSLSAAEMESLAALCDTFLPSVAAAD----------RSAAV--FFETSASTADT-PE 109
S + S +++++L+++CD +P V R+ A+ FF++S+S + P+
Sbjct: 23 SFSHGFSKSDLQALSSICDAIMPPVPLESLNLEMKLKVLRNDALLSFFKSSSSESHVRPD 82
Query: 110 RVGEMIKERVRHPKKWLARLSLWMLSTWYGTYIMCGNKSLSNK-FPYFQRFSKVSQRRRE 169
V E++ + + R+ L +L+ GT ++CG L K +P+ +FS++S +RE
Sbjct: 83 EVAELLATKAIPLTVLVVRIVLRILTFRLGTLLLCGLVCLDKKHWPFLLKFSEMSLEKRE 142
Query: 170 QIVVSWSLSYFF-LLRMFFRTVKIFCLLAFFTQVDDKMNNISWNAIDYPGTDPQFKAKTR 229
+++ W+ ++ L R+ F +K L +FT ++ N W+AI+Y
Sbjct: 143 KVLQRWNTQWYNPLARIGFMMIKAIFLFYYFTWTNENSENPVWDAINY------------ 202
Query: 230 QSEASNLKENGRSREVEEDEEELYGPLFKGIV-NLEHQQVAAAEALRKFGFSVTLLSPRT 289
+ + EN ED E+ PL +GI+ + ++ + + G VT
Sbjct: 203 ---SVEIGEN-------EDMEQKERPLDEGIIETAKEDEMTIKQRMINKGLKVT-----E 262
Query: 290 KASSPSMIIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEE 349
+ I+CDAVVVGSG GGGV A +LA +G +V+V+EKG+Y A + S LEG +M E
Sbjct: 263 DRERDTYKIECDAVVVGSGCGGGVAAAILAKSGLRVVVIEKGNYFAPRDYSALEGPSMFE 322
Query: 350 MYLSSGLVATDNMNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFSSSLYK 409
++ S+ L+ T + +AGSTVGGGS VNW+AS++TP + EWS + ++SS YK
Sbjct: 323 LFESNSLMMTHDGRFRFMAGSTVGGGSVVNWAASLKTPDAIIEEWSVHRGISIYSSEKYK 382
Query: 410 EAMEVVCKKMGVQSEIDNEGFNNAVLRKGCEELGSHVMNIPRNSPSDHYCGWCCLGCKNG 469
AM +VCK++GV +I EGF N +LRKGCE+LG V +PRNS HYCG C GC G
Sbjct: 383 AAMGIVCKRLGVTEKIIREGFQNQILRKGCEKLGLDVTIVPRNSTEKHYCGSCSYGCPTG 442
Query: 470 RKKGTAETWLVDLVRSGNGVILPGCEAIDI------RQEKKDGSERNRAKGVAFEFGCGS 529
K+GT TWLVD V + N VIL C+A + ++++ R R GVA
Sbjct: 443 EKRGTDSTWLVDAV-NNNAVILTQCKAEKLILADNDANKREESGRRKRCLGVAASL--SH 502
Query: 530 GAKQMFLVESKVTVVACGALSTPPLLRKSGLRNPNIGKNLHLHPVTMAWGHFPAETDKSW 589
++ + +KVT+VACG+L TP LL SGL+N NI + LH+HP+ MAWG+FP +
Sbjct: 503 QTRKKLQINAKVTIVACGSLKTPGLLASSGLKNSNISRGLHIHPIMMAWGYFPEKNS--- 562
Query: 590 PEEDKKSYEGGIMTAMCNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWISGTNIKQRM 649
E + ++EG I+T++ + P ++TPA+ PG F+ L PW+SG+++K+RM
Sbjct: 563 -ELEGAAHEGEIVTSLHYVHPMDSTTP---NITLETPAIGPGTFAALTPWVSGSDMKERM 622
Query: 650 CKFSRTAHVFALARDKGSGTIKSPSSFSYNMDDVDEENLQKGLEKALKILAAAGAEEIGT 709
K++RTAH+FA+ RD+G G +K Y + DEENL GL++AL+IL AAGA E+GT
Sbjct: 623 AKYARTAHIFAMVRDEGVGEVKG-DIVKYRLTKADEENLTIGLKQALRILVAAGAAEVGT 682
Query: 710 HHNKGRSINVKKVSYREYEKFVKEESSRP----LKDLMTPICSAHQMGSCRMGIRAKDSV 769
+ + G+ + + ++ E F+ ++ P + T +AHQ+G CRMG K+
Sbjct: 683 YRSDGQRMKCDGIKQKDLEAFLDTVNAPPGVVSMSKHWTQSFTAHQIGCCRMGATEKEGA 742
Query: 770 VNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSVLETLKRRR 796
++ +GE+WE E L++ D+SV PTALGVNPM+TVQ+ AYC + + E +K+R+
Sbjct: 743 IDGKGESWEAEDLYVCDASVLPTALGVNPMITVQSTAYCISNRIAELMKKRK 756
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
O65709 | 2.7e-250 | 58.87 | Long-chain-alcohol oxidase FAO4A OS=Arabidopsis thaliana OX=3702 GN=FAO4A PE=3 S... | [more] |
B5WWZ8 | 8.3e-159 | 41.18 | Long-chain-alcohol oxidase FAO1 OS=Lotus japonicus OX=34305 GN=FAO1 PE=1 SV=1 | [more] |
B5WWZ9 | 3.7e-151 | 39.82 | Long-chain-alcohol oxidase FAO2 OS=Lotus japonicus OX=34305 GN=FAO2 PE=2 SV=1 | [more] |
Q94BP3 | 1.5e-147 | 40.40 | Long-chain-alcohol oxidase FAO4B OS=Arabidopsis thaliana OX=3702 GN=FAO4B PE=2 S... | [more] |
Q9LW56 | 7.3e-147 | 40.45 | Long-chain-alcohol oxidase FAO3 OS=Arabidopsis thaliana OX=3702 GN=FAO3 PE=1 SV=... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1FFC0 | 0.0e+00 | 87.92 | Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111443434 PE=3 SV... | [more] |
A0A6J1IPF0 | 0.0e+00 | 87.04 | Long-chain-alcohol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111477017 PE=3 SV=1 | [more] |
A0A6J1FQT3 | 0.0e+00 | 86.19 | Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111446403 PE=3 SV... | [more] |
A0A1S3BAP1 | 0.0e+00 | 84.29 | Long-chain-alcohol oxidase OS=Cucumis melo OX=3656 GN=LOC103487628 PE=3 SV=1 | [more] |
A0A6J1CNU3 | 0.0e+00 | 81.83 | Long-chain-alcohol oxidase OS=Momordica charantia OX=3673 GN=LOC111012719 PE=3 S... | [more] |
Match Name | E-value | Identity | Description | |
XP_038896170.1 | 0.0e+00 | 86.53 | long-chain-alcohol oxidase FAO4A [Benincasa hispida] | [more] |
XP_022937010.1 | 0.0e+00 | 87.92 | long-chain-alcohol oxidase FAO4A-like [Cucurbita moschata] | [more] |
XP_023535226.1 | 0.0e+00 | 88.30 | long-chain-alcohol oxidase FAO4A-like [Cucurbita pepo subsp. pepo] | [more] |
KAG6591858.1 | 0.0e+00 | 87.30 | Long-chain-alcohol oxidase FAO4A, partial [Cucurbita argyrosperma subsp. sororia... | [more] |
XP_023523874.1 | 0.0e+00 | 86.90 | long-chain-alcohol oxidase FAO4A-like [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
AT4G19380.1 | 1.9e-251 | 58.87 | Long-chain fatty alcohol dehydrogenase family protein | [more] |
AT4G28570.1 | 1.0e-148 | 40.40 | Long-chain fatty alcohol dehydrogenase family protein | [more] |
AT3G23410.1 | 5.2e-148 | 40.45 | fatty alcohol oxidase 3 | [more] |
AT1G03990.1 | 4.0e-140 | 37.05 | Long-chain fatty alcohol dehydrogenase family protein | [more] |