Lcy12g011300 (gene) Sponge gourd (P93075) v1

BLAST of Lcy12g011300 vs. ExPASy Swiss-Prot
Match: F4IEM5 (LanC-like protein GCR2 OS=Arabidopsis thaliana OX=3702 GN=GCR2 PE=1 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 1.2e-166
Identity = 282/414 (68.12%), Postives = 333/414 (80.43%), Query Frame = 0

Query: 1   MPDRFFPNRMPHFVPEAPADELAPSDSDIAEDPLTPTELLSLPHAALSDRLRQTALDLKE 60
           M +RFF N MP FVPE    +L+  +  + E   + T+LLSLP+ + S++L + AL +K+
Sbjct: 1   MGERFFRNEMPEFVPE----DLSGEEETVTECKDSLTKLLSLPYKSFSEKLHRYALSIKD 60

Query: 61  TVVRETWTLSGRYPRDYTLYTGALGTAFLALKSYLVSNNENDLKLCSEIVRACETVSRDS 120
            VV ETW  SG+  RDY LYTG LGTA+L  KSY V+ NE+DLKLC E V AC+  SRDS
Sbjct: 61  KVVWETWERSGKRVRDYNLYTGVLGTAYLLFKSYQVTRNEDDLKLCLENVEACDVASRDS 120

Query: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLLDHYLGNFKDIKLPSDLPNELLYGRAGFLWAC 180
            RV+F+CG AGVCALGAVAAK   D +L D YL  F+ I+LPSDLP ELLYGRAG+LWAC
Sbjct: 121 ERVTFICGYAGVCALGAVAAKCLGDDQLYDRYLARFRGIRLPSDLPYELLYGRAGYLWAC 180

Query: 181 LFLNKHIGQNSISNTIMRSVVGEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMH 240
           LFLNKHIGQ SIS+  MRSVV E+  AGRQLG KG  PLMYEWHGK+YWGAAHGLAGIM+
Sbjct: 181 LFLNKHIGQESISSERMRSVVEEIFRAGRQLGNKGTCPLMYEWHGKRYWGAAHGLAGIMN 240

Query: 241 VLMHMELKPDEVKDVKNTLRYMIKNRFPSGNYCSSEGSESDRLVHWCHGAPGVALTLIKA 300
           VLMH EL+PDE+KDVK TL YMI+NRFPSGNY SSEGS+SDRLVHWCHGAPGVALTL+KA
Sbjct: 241 VLMHTELEPDEIKDVKGTLSYMIQNRFPSGNYLSSEGSKSDRLVHWCHGAPGVALTLVKA 300

Query: 301 AEVFGDDEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTRYLYRAKA 360
           A+V+   EF++AA++AGEVVW+RGLLKRVGICHGISGNTYVFLSLYRLT + +YLYRAKA
Sbjct: 301 AQVYNTKEFVEAAMEAGEVVWSRGLLKRVGICHGISGNTYVFLSLYRLTRNPKYLYRAKA 360

Query: 361 FACFLHQNAQKLISEGKMHSGDRPYSLFEGIGGMTYLFFDMIEPSASRFPSYEL 415
           FA FL   ++KLISEG+MH GDRP+SLFEGIGGM Y+  DM +P+ + FP YEL
Sbjct: 361 FASFLLDKSEKLISEGQMHGGDRPFSLFEGIGGMAYMLLDMNDPTQALFPGYEL 410

BLAST of Lcy12g011300 vs. ExPASy Swiss-Prot
Match: Q8VZQ6 (LanC-like protein GCL2 OS=Arabidopsis thaliana OX=3702 GN=GCL2 PE=2 SV=1)

HSP 1 Score: 542.7 bits (1397), Expect = 3.5e-153
Identity = 262/415 (63.13%), Postives = 321/415 (77.35%), Query Frame = 0

Query: 1   MPDRFFPNRMPHFVPEAPADELAPSDSDIAEDPLTPTELLSLPHAALSDRLRQTALDLKE 60
           M  RFF N MP FV E   + ++  D        T   LL++P+++LS +L+++ALDLKE
Sbjct: 1   MAGRFFDNVMPDFVKE--KESVSGGD--------TLRNLLAMPYSSLSQQLKRSALDLKE 60

Query: 61  TVVRETWTLSGRYPRDYTLYTGALGTAFLALKSYLVSNNENDLKLCSEIVRACETVSRDS 120
           TVV ETW  SG+   D+TLY+G LG AFL  ++Y V+ N NDL LC EIV+AC+T S  S
Sbjct: 61  TVVIETWGFSGQTVEDFTLYSGTLGAAFLLFRAYQVTGNANDLSLCLEIVKACDTASASS 120

Query: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLLDHYLGNFKDIKLPSDLPNELLYGRAGFLWAC 180
             V+FLCGRAGVC LGAVAAKL+ +  LL++YLG F+ I+L SDLPNELLYGR G+LWAC
Sbjct: 121 GDVTFLCGRAGVCGLGAVAAKLSGEEDLLNYYLGQFRLIRLSSDLPNELLYGRVGYLWAC 180

Query: 181 LFLNKHIGQNSISNTIMRSVVGEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMH 240
           LF+NK+IG+ ++S+  +R V  E+I+ GR + KKG SPLM+EW+GK+YWGAAHGLAGIMH
Sbjct: 181 LFINKYIGKETLSSDTIREVAQEIIKEGRSMAKKGSSPLMFEWYGKRYWGAAHGLAGIMH 240

Query: 241 VLMHMELKPDEVKDVKNTLRYMIKNRFPSGNY-CSSEGSESDRLVHWCHGAPGVALTLIK 300
           VLM ++LKPDE +DVK TL+YMIKNRFPSGNY  S E  + D LVHWCHGAPG+ALTL K
Sbjct: 241 VLMDVQLKPDEAEDVKGTLKYMIKNRFPSGNYPASEEDKKKDILVHWCHGAPGIALTLGK 300

Query: 301 AAEVFGDDEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTRYLYRAK 360
           AAEVFG+ EFL+A+  A EVVWNRGLLKRVGICHGISGN YVFL+LYR TG + YLYRAK
Sbjct: 301 AAEVFGEREFLEASAAAAEVVWNRGLLKRVGICHGISGNAYVFLALYRATGRSEYLYRAK 360

Query: 361 AFACFLHQNAQKLISEGKMHSGDRPYSLFEGIGGMTYLFFDMIEPSASRFPSYEL 415
           AFA FL     KL+S+G+MH GD PYSLFEG+ GM YLF DM++PS +RFP YEL
Sbjct: 361 AFASFLLDRGPKLLSKGEMHGGDSPYSLFEGVAGMAYLFLDMVDPSEARFPGYEL 405

BLAST of Lcy12g011300 vs. ExPASy Swiss-Prot
Match: Q9FJN7 (LanC-like protein GCL1 OS=Arabidopsis thaliana OX=3702 GN=GCL1 PE=2 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 4.4e-95
Identity = 187/408 (45.83%), Postives = 261/408 (63.97%), Query Frame = 0

Query: 29  IAEDPLTPTELLSLPHAALSDRLRQTALDLKETVVRETW------TLSGRYP-RDYTLYT 88
           +  +P  PT  +SLP    ++   + A  LK  VV  TW        SG  P  D T+YT
Sbjct: 33  LLSEPSAPT--ISLP----TESFLRAATLLKNQVVEATWKGGVEALASGSGPVLDPTVYT 92

Query: 89  GALGTAFLALKSYLVSNNENDLKLCSEIVRACETVSR-DSRRVSFLCGRAGVCALGAVAA 148
           G LGTAF  LKSY V+ N  DL  C+EI+  C  V+R  +R V+FLCGR GVC LGA+ A
Sbjct: 93  GLLGTAFTCLKSYEVTRNHQDLLTCAEIIDTCANVARATTRHVTFLCGRGGVCTLGAIVA 152

Query: 149 KLANDGRLLDHYLGNFKDIKLPSDLP-----------NELLYGRAGFLWACLFLNKHIGQ 208
               D    D +LG F ++    +LP            +LLYGRAGFLWA LFLN+++GQ
Sbjct: 153 NYRGDQSKRDFFLGLFLELAEERELPAGPEEGGFGMSYDLLYGRAGFLWAALFLNRYLGQ 212

Query: 209 NSISNTIMRSVVGEVIEAGRQLGKKGKS-PLMYEWHGKKYWGAAHGLAGIMHVLMHMELK 268
            ++ + ++  +V  ++  GR      ++ PL+Y +HG ++WGAA+GLAGI++VL+H  L 
Sbjct: 213 GTVPDHLLSPIVAAILAGGRVGAADHEACPLLYRFHGTRFWGAANGLAGILYVLLHFPLS 272

Query: 269 PDEVKDVKNTLRYMIKNRFP-SGNYCSSEGSESDRLVHWCHGAPGVALTLIKAAEVFGDD 328
            ++VKDV+ TLRYM+ NRFP SGNY  SEG+  D+LV W HGA G+A+TL KA++VF  +
Sbjct: 273 EEDVKDVQGTLRYMMSNRFPNSGNYPCSEGNPRDKLVQWAHGATGMAITLAKASQVFPKE 332

Query: 329 -EFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTRYLYRAKAFACFLH 388
            +F +AA++AGEVVW  GL+K+VG+  G++GN Y FLSLYRLTGD  Y  RAKAFA +L 
Sbjct: 333 RDFREAAIEAGEVVWKSGLVKKVGLADGVAGNAYAFLSLYRLTGDVVYEERAKAFASYLC 392

Query: 389 QNAQKLISEGKMHSGDRPYSLFEGIGGMTYLFFDMIEPSASRFPSYEL 415
           ++A +L++     + +  YSLF G+ G   L+FD++ P  S+FP YE+
Sbjct: 393 RDAIELVNMTSQET-EHDYSLFRGLAGPVCLWFDLVSPVDSKFPGYEI 433

BLAST of Lcy12g011300 vs. ExPASy Swiss-Prot
Match: Q9JJK2 (LanC-like protein 2 OS=Mus musculus OX=10090 GN=Lancl2 PE=1 SV=1)

HSP 1 Score: 283.9 bits (725), Expect = 3.0e-75
Identity = 175/439 (39.86%), Postives = 254/439 (57.86%), Query Frame = 0

Query: 1   MPDRFFPNRMPHFVPEAPADELAPSDSDIAED--PLTPTELLSLPH----AALSDRLRQT 60
           M +R FPN  P +   A A  LA   ++      PL  TE   LP       + + +++ 
Sbjct: 18  MEERSFPNPFPDYEAAASAAGLAAGSAEETGRVCPLPTTEDPGLPFHPNGKIVPNFIKRI 77

Query: 61  ALDLKETVVRETWTLSGRYPRDYTLYTGALGTAFLALKSYLVSNNENDLKLCSEIVRACE 120
              +K+ + +    L    P D + YTG  G A L L+ Y V+ ++      + ++R+ +
Sbjct: 78  QTKIKDLLQQMEEGLKTADPHDCSAYTGWTGIALLYLQLYRVTGDQ------TYLLRSLD 137

Query: 121 TVSR-----DSRRVSFLCGRAGVCALGAVA-AKLAND---GRLLDHYLGNFKDIKL-PSD 180
            V R       RRV+FLCG AG  A+GAV   KL ++      +   L   + I    S+
Sbjct: 138 YVKRTLRNLSGRRVTFLCGDAGPLAVGAVIYHKLKSECESQECITKLLQMHRTIVCQESE 197

Query: 181 LPNELLYGRAGFLWACLFLNKHIGQNSISNTIMRSVVGEVIEAGRQLG----KKGKSPLM 240
           LP+ELLYGRAG+L+A L+LN  IG  ++  T ++ VV  +IE+G+ L     K  + PL+
Sbjct: 198 LPDELLYGRAGYLYALLYLNTEIGPGTVGETAIKEVVSAIIESGKSLSREERKSERCPLL 257

Query: 241 YEWHGKKYWGAAHGLAGIMHVLMHMELKPDE---VKDVKNTLRYMIKNRFPSGNYCSSEG 300
           Y+WH K+Y GAAHG+AGI ++LM  E K D+    + VK ++ Y+   +F SGNY SS  
Sbjct: 258 YQWHRKQYVGAAHGMAGIYYMLMQPEAKVDQETLTEMVKPSIDYVRHKKFRSGNYPSSLS 317

Query: 301 SESDRLVHWCHGAPGVALTLIKAAEVFGDDEFLQAALDAGEVVWNRGLLKR-VGICHGIS 360
           +E+DRLVHWCHGAPGV   L++A +VF ++++L+ A++  +V+W RGLL++  GICHG S
Sbjct: 318 NETDRLVHWCHGAPGVIHVLLQAYQVFKEEKYLKEAMECSDVIWQRGLLRKGYGICHGTS 377

Query: 361 GNTYVFLSLYRLTGDTRYLYRAKAFACF-LHQNAQKLISEGKMHSGDRPYSLFEGIGGMT 415
           GN Y FLSLYRLT D +YLYRA  FA + L   A            DRPYSLFEG+ G  
Sbjct: 378 GNGYSFLSLYRLTQDKKYLYRACKFAEWCLDYGAH------GCRIPDRPYSLFEGMAGAV 437

BLAST of Lcy12g011300 vs. ExPASy Swiss-Prot
Match: Q9NS86 (LanC-like protein 2 OS=Homo sapiens OX=9606 GN=LANCL2 PE=1 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.5e-71
Identity = 175/442 (39.59%), Postives = 255/442 (57.69%), Query Frame = 0

Query: 1   MPDRFFPNRMPHFVPEAPADELAPSDSDIAED-----PLTPTELLSLPH----AALSDRL 60
           M +R F N  P +  EA A  L  S +  AE+     P   T+   LP       + + +
Sbjct: 19  MEERAFVNPFPDY--EAAAGALLASGA--AEETGCVRPPATTDEPGLPFHQDGKIIHNFI 78

Query: 61  RQTALDLKETVVRETWTLSGRYPRDYTLYTGALGTAFLALKSYLVSNNENDLKLCSEIVR 120
           R+    +K+ + +    L    P D + YTG  G A L L+ Y V+ ++      + ++R
Sbjct: 79  RRIQTKIKDLLQQMEEGLKTADPHDCSAYTGWTGIALLYLQLYRVTCDQ------TYLLR 138

Query: 121 ACETVSR-----DSRRVSFLCGRAGVCALGAVA-AKLAND---GRLLDHYLGNFKDIKL- 180
           + + V R     + RRV+FLCG AG  A+GAV   KL +D      +   L   + +   
Sbjct: 139 SLDYVKRTLRNLNGRRVTFLCGDAGPLAVGAVIYHKLRSDCESQECVTKLLQLQRSVVCQ 198

Query: 181 PSDLPNELLYGRAGFLWACLFLNKHIGQNSISNTIMRSVVGEVIEAGRQLG----KKGKS 240
            SDLP+ELLYGRAG+L+A L+LN  IG  ++  + ++ VV  +IE+G+ L     K  + 
Sbjct: 199 ESDLPDELLYGRAGYLYALLYLNTEIGPGTVCESAIKEVVNAIIESGKTLSREERKTERC 258

Query: 241 PLMYEWHGKKYWGAAHGLAGIMHVLMHMELKPDE---VKDVKNTLRYMIKNRFPSGNYCS 300
           PL+Y+WH K+Y GAAHG+AGI ++LM    K D+    + VK ++ Y+   +F SGNY S
Sbjct: 259 PLLYQWHRKQYVGAAHGMAGIYYMLMQPAAKVDQETLTEMVKPSIDYVRHKKFRSGNYPS 318

Query: 301 SEGSESDRLVHWCHGAPGVALTLIKAAEVFGDDEFLQAALDAGEVVWNRGLLKR-VGICH 360
           S  +E+DRLVHWCHGAPGV   L++A +VF ++++L+ A++  +V+W RGLL++  GICH
Sbjct: 319 SLSNETDRLVHWCHGAPGVIHMLMQAYKVFKEEKYLKEAMECSDVIWQRGLLRKGYGICH 378

Query: 361 GISGNTYVFLSLYRLTGDTRYLYRAKAFACF-LHQNAQKLISEGKMHSGDRPYSLFEGIG 415
           G +GN Y FLSLYRLT D +YLYRA  FA + L   A            DRPYSLFEG+ 
Sbjct: 379 GTAGNGYSFLSLYRLTQDKKYLYRACKFAEWCLDYGAH------GCRIPDRPYSLFEGMA 438

BLAST of Lcy12g011300 vs. ExPASy TrEMBL
Match: A0A6J1IZ37 (lanC-like protein GCR2 OS=Cucurbita maxima OX=3661 GN=LOC111480469 PE=3 SV=1)

HSP 1 Score: 755.7 bits (1950), Expect = 9.8e-215
Identity = 368/414 (88.89%), Postives = 389/414 (93.96%), Query Frame = 0

Query: 1   MPDRFFPNRMPHFVPEAPADELAPSDSDIAEDPLTPTELLSLPHAALSDRLRQTALDLKE 60
           M DRFFPN+MPHFV EAPADELAPSDSDIA DPLTP++LLSLPHAALS R  QTALDLKE
Sbjct: 1   MADRFFPNQMPHFVAEAPADELAPSDSDIAGDPLTPSKLLSLPHAALSARFIQTALDLKE 60

Query: 61  TVVRETWTLSGRYPRDYTLYTGALGTAFLALKSYLVSNNENDLKLCSEIVRACETVSRDS 120
           TVVR  WTLS RYP+DYTLYTGALGTAFLALKSY+VSNNENDLKLCSEIVRACET+S DS
Sbjct: 61  TVVRGMWTLSERYPQDYTLYTGALGTAFLALKSYVVSNNENDLKLCSEIVRACETLSGDS 120

Query: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLLDHYLGNFKDIKLPSDLPNELLYGRAGFLWAC 180
           RRVSFLCGRAGVCALGAVAAKLANDGRL+DHYL  FK IKLPSDLPNELLYGRAGFLWAC
Sbjct: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLVDHYLEKFKHIKLPSDLPNELLYGRAGFLWAC 180

Query: 181 LFLNKHIGQNSISNTIMRSVVGEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMH 240
           LFLNKHI Q++IS TIMR+VV EVI+AGRQLGK GKSPLMYEWHGKKYWGAAHGLAGIMH
Sbjct: 181 LFLNKHICQHTISKTIMRAVVDEVIKAGRQLGKNGKSPLMYEWHGKKYWGAAHGLAGIMH 240

Query: 241 VLMHMELKPDEVKDVKNTLRYMIKNRFPSGNYCSSEGSESDRLVHWCHGAPGVALTLIKA 300
           VLM+MELKPDEV+DVKNTLRYMIKNRFPSGN+ SSEG++SD+LVHWCHGAPGVALTLIKA
Sbjct: 241 VLMNMELKPDEVEDVKNTLRYMIKNRFPSGNFHSSEGNDSDKLVHWCHGAPGVALTLIKA 300

Query: 301 AEVFGDDEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTRYLYRAKA 360
           AEVFGD EFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGD  YLYRAKA
Sbjct: 301 AEVFGDSEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDPVYLYRAKA 360

Query: 361 FACFLHQNAQKLISEGKMHSGDRPYSLFEGIGGMTYLFFDMIEPSASRFPSYEL 415
           FACFLHQNAQ LISEGKM SGDRP+S+FEGIGGM YLFFDM EPSA+RFP+YEL
Sbjct: 361 FACFLHQNAQMLISEGKMQSGDRPFSMFEGIGGMAYLFFDMNEPSAARFPAYEL 414

BLAST of Lcy12g011300 vs. ExPASy TrEMBL
Match: A0A6J1FLL9 (lanC-like protein GCR2 OS=Cucurbita moschata OX=3662 GN=LOC111446345 PE=3 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 2.8e-214
Identity = 368/414 (88.89%), Postives = 389/414 (93.96%), Query Frame = 0

Query: 1   MPDRFFPNRMPHFVPEAPADELAPSDSDIAEDPLTPTELLSLPHAALSDRLRQTALDLKE 60
           M DRFFP +MPHFV EAPADELAPSDSDI EDPLTP++LLSLPHAALS R  QTALDLKE
Sbjct: 1   MADRFFPIQMPHFVAEAPADELAPSDSDIDEDPLTPSKLLSLPHAALSARFIQTALDLKE 60

Query: 61  TVVRETWTLSGRYPRDYTLYTGALGTAFLALKSYLVSNNENDLKLCSEIVRACETVSRDS 120
           TVVRETWTLS RY +DYTLYTGALGTAFLALKSY+VSNNENDLKLCSEIVRACET+SRDS
Sbjct: 61  TVVRETWTLSERYLQDYTLYTGALGTAFLALKSYVVSNNENDLKLCSEIVRACETISRDS 120

Query: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLLDHYLGNFKDIKLPSDLPNELLYGRAGFLWAC 180
           RRVSFLCGRAGVCALGAVAAKLANDGRL+DHYL  FK IKLPS+LPNELLYGRAGFLWAC
Sbjct: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLVDHYLEKFKHIKLPSNLPNELLYGRAGFLWAC 180

Query: 181 LFLNKHIGQNSISNTIMRSVVGEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMH 240
           LFLNKHI Q++IS TIMRSVV EVI+ GRQLGK GKSPLMYEWHGKKYWGAAHGLAGIMH
Sbjct: 181 LFLNKHICQHTISKTIMRSVVDEVIKVGRQLGKNGKSPLMYEWHGKKYWGAAHGLAGIMH 240

Query: 241 VLMHMELKPDEVKDVKNTLRYMIKNRFPSGNYCSSEGSESDRLVHWCHGAPGVALTLIKA 300
           VLM+MELKPDEV+DVKNTLRYMIKNRFPSGN+ SSE ++SD+LVHWCHGAPGVALTLIKA
Sbjct: 241 VLMNMELKPDEVEDVKNTLRYMIKNRFPSGNFRSSEENDSDKLVHWCHGAPGVALTLIKA 300

Query: 301 AEVFGDDEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTRYLYRAKA 360
           AEVFGD EFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGD  YLYRAKA
Sbjct: 301 AEVFGDSEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDPVYLYRAKA 360

Query: 361 FACFLHQNAQKLISEGKMHSGDRPYSLFEGIGGMTYLFFDMIEPSASRFPSYEL 415
           FACFLHQNAQKLISEGKM SGDRP+S+FEGIGGM YLFFDM EPSA+RFP+YEL
Sbjct: 361 FACFLHQNAQKLISEGKMQSGDRPFSMFEGIGGMAYLFFDMNEPSAARFPAYEL 414

BLAST of Lcy12g011300 vs. ExPASy TrEMBL
Match: A0A0A0L279 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G638330 PE=3 SV=1)

HSP 1 Score: 732.3 bits (1889), Expect = 1.2e-207
Identity = 358/414 (86.47%), Postives = 379/414 (91.55%), Query Frame = 0

Query: 1   MPDRFFPNRMPHFVPEAPADELAPSDSDIAEDPLTPTELLSLPHAALSDRLRQTALDLKE 60
           M DRFFPN+MP F  EAP DELA S S  A   L PTELLSLP AALSDRLRQ AL+LKE
Sbjct: 1   MADRFFPNQMPRFPAEAPPDELASSHSYSA--TLIPTELLSLPDAALSDRLRQAALNLKE 60

Query: 61  TVVRETWTLSGRYPRDYTLYTGALGTAFLALKSYLVSNNENDLKLCSEIVRACETVSRDS 120
           TVVRETWTLSGR+P+D TLYTGALGTAFLALKS+ VS NENDLKLCSEIV ACE++S+ S
Sbjct: 61  TVVRETWTLSGRHPQDNTLYTGALGTAFLALKSFFVSKNENDLKLCSEIVTACESLSKTS 120

Query: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLLDHYLGNFKDIKLPSDLPNELLYGRAGFLWAC 180
           R V+F+CGRAGVCALGAVAAK ANDGRL+DHYL  FKDIKLPSDLPNELLYGRAGFLWAC
Sbjct: 121 RHVTFICGRAGVCALGAVAAKFANDGRLVDHYLAKFKDIKLPSDLPNELLYGRAGFLWAC 180

Query: 181 LFLNKHIGQNSISNTIMRSVVGEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMH 240
           LFLNKHIGQN+ISN  MRSVV EVIEAGR LG+K KSPLMYEWHGKKYWGAAHGLAGIMH
Sbjct: 181 LFLNKHIGQNTISNNFMRSVVDEVIEAGRTLGEKSKSPLMYEWHGKKYWGAAHGLAGIMH 240

Query: 241 VLMHMELKPDEVKDVKNTLRYMIKNRFPSGNYCSSEGSESDRLVHWCHGAPGVALTLIKA 300
           VLM MELKPDEV+DVKNTLRYMIKNRFPSGNY SSEGSESDRLVHWCHGAPGVALTL KA
Sbjct: 241 VLMDMELKPDEVEDVKNTLRYMIKNRFPSGNYPSSEGSESDRLVHWCHGAPGVALTLGKA 300

Query: 301 AEVFGDDEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTRYLYRAKA 360
           AEVFGDDEFLQAA+DAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGD  YL+RAKA
Sbjct: 301 AEVFGDDEFLQAAIDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDLMYLHRAKA 360

Query: 361 FACFLHQNAQKLISEGKMHSGDRPYSLFEGIGGMTYLFFDMIEPSASRFPSYEL 415
           FACFLHQNA+KLISEGKMHSGDRPYSLFEGIGGM YLFFDM+EP+A++FPSYEL
Sbjct: 361 FACFLHQNAEKLISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEPNAAKFPSYEL 412

BLAST of Lcy12g011300 vs. ExPASy TrEMBL
Match: A0A6J1CCV8 (lanC-like protein GCR2 OS=Momordica charantia OX=3673 GN=LOC111010494 PE=3 SV=1)

HSP 1 Score: 722.6 bits (1864), Expect = 9.2e-205
Identity = 347/414 (83.82%), Postives = 379/414 (91.55%), Query Frame = 0

Query: 1   MPDRFFPNRMPHFVPEAPADELAPSDSDIAEDPLTPTELLSLPHAALSDRLRQTALDLKE 60
           M DRFFPN+MP FV EAPA+ELA SDS    D LT TELLSLP+AALSD  R+ A DLKE
Sbjct: 1   MADRFFPNQMPDFVAEAPAEELAASDS----DALTLTELLSLPYAALSDHFRKAAFDLKE 60

Query: 61  TVVRETWTLSGRYPRDYTLYTGALGTAFLALKSYLVSNNENDLKLCSEIVRACETVSRDS 120
           TVVRETWT    +PRD+TLYTGALGTAFLALKSYLVSNNE DLKLCSEIV AC++VS DS
Sbjct: 61  TVVRETWTSKESHPRDWTLYTGALGTAFLALKSYLVSNNETDLKLCSEIVTACDSVSTDS 120

Query: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLLDHYLGNFKDIKLPSDLPNELLYGRAGFLWAC 180
           RRVSF+CGRAGVCALGAVAAKLAN+GR + HYL  F+ IK P DLPNELLYGRAGFLWAC
Sbjct: 121 RRVSFICGRAGVCALGAVAAKLANNGRQVSHYLEKFEYIKPPDDLPNELLYGRAGFLWAC 180

Query: 181 LFLNKHIGQNSISNTIMRSVVGEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMH 240
           LFLNKHIG N+ISNTIMRSVV EVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMH
Sbjct: 181 LFLNKHIGHNTISNTIMRSVVDEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMH 240

Query: 241 VLMHMELKPDEVKDVKNTLRYMIKNRFPSGNYCSSEGSESDRLVHWCHGAPGVALTLIKA 300
           VLM+++L+PDE +DVK+T+RYMIK+RFPSGNYCSSEG+ESDRLVHWCHGAPGVALTLIKA
Sbjct: 241 VLMNVKLQPDEAEDVKSTIRYMIKDRFPSGNYCSSEGNESDRLVHWCHGAPGVALTLIKA 300

Query: 301 AEVFGDDEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTRYLYRAKA 360
            E+FGDD+FL+AA+DA EV+WNRGLLKRVGICHGISGNTYVFLSLYRLTGDT+YLYRAKA
Sbjct: 301 VEIFGDDKFLEAAMDAAEVIWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTKYLYRAKA 360

Query: 361 FACFLHQNAQKLISEGKMHSGDRPYSLFEGIGGMTYLFFDMIEPSASRFPSYEL 415
           FACFLHQNA++LISEGKMHSGDRPYSLFEGIGGM YLFFDM+EPS +RFPSYEL
Sbjct: 361 FACFLHQNAERLISEGKMHSGDRPYSLFEGIGGMAYLFFDMVEPSVARFPSYEL 410

BLAST of Lcy12g011300 vs. ExPASy TrEMBL
Match: A0A5A7V883 (LanC-like protein GCL2 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003830 PE=3 SV=1)

HSP 1 Score: 717.2 bits (1850), Expect = 3.9e-203
Identity = 353/414 (85.27%), Postives = 375/414 (90.58%), Query Frame = 0

Query: 1   MPDRFFPNRMPHFVPEAPADELAPSDSDIAEDPLTPTELLSLPHAALSDRLRQTALDLKE 60
           M DRFFPN MP F  EAP+DEL  S S  A   L PTELLSLP AAL DRLRQTAL+LKE
Sbjct: 1   MADRFFPNPMPRFPAEAPSDELVSSHSYSA--TLIPTELLSLPDAALFDRLRQTALNLKE 60

Query: 61  TVVRETWTLSGRYPRDYTLYTGALGTAFLALKSYLVSNNENDLKLCSEIVRACETVSRDS 120
           TVVRETWTLSGR+P+DYTLYTGALGTAFLALKS+LV  NENDLKLCSEIV ACE++S+ S
Sbjct: 61  TVVRETWTLSGRHPQDYTLYTGALGTAFLALKSFLVFKNENDLKLCSEIVAACESLSKKS 120

Query: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLLDHYLGNFKDIKLPSDLPNELLYGRAGFLWAC 180
           R V+F+CGRAGVCALGAVAAK ANDG L+DHYL  FKDIKLPSDLPNELLYGRAGFLWAC
Sbjct: 121 RHVTFICGRAGVCALGAVAAKFANDGMLVDHYLEKFKDIKLPSDLPNELLYGRAGFLWAC 180

Query: 181 LFLNKHIGQNSISNTIMRSVVGEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMH 240
           LFLNKHIG+N+ISNT MRSVV EVIEAGR+LG+K KSPLMYEWHGKKYWGAAHGLAGIMH
Sbjct: 181 LFLNKHIGRNTISNTFMRSVVDEVIEAGRRLGQKSKSPLMYEWHGKKYWGAAHGLAGIMH 240

Query: 241 VLMHMELKPDEVKDVKNTLRYMIKNRFPSGNYCSSEGSESDRLVHWCHGAPGVALTLIKA 300
           VLM MELKPDEV+DVKNTLRYMIKNRF SGNY SSE SESDRLVHWCHGAPGVALTL KA
Sbjct: 241 VLMDMELKPDEVEDVKNTLRYMIKNRFLSGNYPSSEESESDRLVHWCHGAPGVALTLGKA 300

Query: 301 AEVFGDDEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTRYLYRAKA 360
           AEVFGDDEFLQAA+DAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGD  YL+RAKA
Sbjct: 301 AEVFGDDEFLQAAIDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDLTYLHRAKA 360

Query: 361 FACFLHQNAQKLISEGKMHSGDRPYSLFEGIGGMTYLFFDMIEPSASRFPSYEL 415
           FACFLHQNA+KLISEGKMHSGD PYSLFEGIGGM YLF DM+EP A++FPSYEL
Sbjct: 361 FACFLHQNAEKLISEGKMHSGDCPYSLFEGIGGMAYLFLDMVEPYAAKFPSYEL 412

BLAST of Lcy12g011300 vs. NCBI nr
Match: XP_022981295.1 (lanC-like protein GCR2 [Cucurbita maxima])

HSP 1 Score: 755.7 bits (1950), Expect = 2.0e-214
Identity = 368/414 (88.89%), Postives = 389/414 (93.96%), Query Frame = 0

Query: 1   MPDRFFPNRMPHFVPEAPADELAPSDSDIAEDPLTPTELLSLPHAALSDRLRQTALDLKE 60
           M DRFFPN+MPHFV EAPADELAPSDSDIA DPLTP++LLSLPHAALS R  QTALDLKE
Sbjct: 1   MADRFFPNQMPHFVAEAPADELAPSDSDIAGDPLTPSKLLSLPHAALSARFIQTALDLKE 60

Query: 61  TVVRETWTLSGRYPRDYTLYTGALGTAFLALKSYLVSNNENDLKLCSEIVRACETVSRDS 120
           TVVR  WTLS RYP+DYTLYTGALGTAFLALKSY+VSNNENDLKLCSEIVRACET+S DS
Sbjct: 61  TVVRGMWTLSERYPQDYTLYTGALGTAFLALKSYVVSNNENDLKLCSEIVRACETLSGDS 120

Query: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLLDHYLGNFKDIKLPSDLPNELLYGRAGFLWAC 180
           RRVSFLCGRAGVCALGAVAAKLANDGRL+DHYL  FK IKLPSDLPNELLYGRAGFLWAC
Sbjct: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLVDHYLEKFKHIKLPSDLPNELLYGRAGFLWAC 180

Query: 181 LFLNKHIGQNSISNTIMRSVVGEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMH 240
           LFLNKHI Q++IS TIMR+VV EVI+AGRQLGK GKSPLMYEWHGKKYWGAAHGLAGIMH
Sbjct: 181 LFLNKHICQHTISKTIMRAVVDEVIKAGRQLGKNGKSPLMYEWHGKKYWGAAHGLAGIMH 240

Query: 241 VLMHMELKPDEVKDVKNTLRYMIKNRFPSGNYCSSEGSESDRLVHWCHGAPGVALTLIKA 300
           VLM+MELKPDEV+DVKNTLRYMIKNRFPSGN+ SSEG++SD+LVHWCHGAPGVALTLIKA
Sbjct: 241 VLMNMELKPDEVEDVKNTLRYMIKNRFPSGNFHSSEGNDSDKLVHWCHGAPGVALTLIKA 300

Query: 301 AEVFGDDEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTRYLYRAKA 360
           AEVFGD EFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGD  YLYRAKA
Sbjct: 301 AEVFGDSEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDPVYLYRAKA 360

Query: 361 FACFLHQNAQKLISEGKMHSGDRPYSLFEGIGGMTYLFFDMIEPSASRFPSYEL 415
           FACFLHQNAQ LISEGKM SGDRP+S+FEGIGGM YLFFDM EPSA+RFP+YEL
Sbjct: 361 FACFLHQNAQMLISEGKMQSGDRPFSMFEGIGGMAYLFFDMNEPSAARFPAYEL 414

BLAST of Lcy12g011300 vs. NCBI nr
Match: XP_022940894.1 (lanC-like protein GCR2 [Cucurbita moschata])

HSP 1 Score: 754.2 bits (1946), Expect = 5.9e-214
Identity = 368/414 (88.89%), Postives = 389/414 (93.96%), Query Frame = 0

Query: 1   MPDRFFPNRMPHFVPEAPADELAPSDSDIAEDPLTPTELLSLPHAALSDRLRQTALDLKE 60
           M DRFFP +MPHFV EAPADELAPSDSDI EDPLTP++LLSLPHAALS R  QTALDLKE
Sbjct: 1   MADRFFPIQMPHFVAEAPADELAPSDSDIDEDPLTPSKLLSLPHAALSARFIQTALDLKE 60

Query: 61  TVVRETWTLSGRYPRDYTLYTGALGTAFLALKSYLVSNNENDLKLCSEIVRACETVSRDS 120
           TVVRETWTLS RY +DYTLYTGALGTAFLALKSY+VSNNENDLKLCSEIVRACET+SRDS
Sbjct: 61  TVVRETWTLSERYLQDYTLYTGALGTAFLALKSYVVSNNENDLKLCSEIVRACETISRDS 120

Query: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLLDHYLGNFKDIKLPSDLPNELLYGRAGFLWAC 180
           RRVSFLCGRAGVCALGAVAAKLANDGRL+DHYL  FK IKLPS+LPNELLYGRAGFLWAC
Sbjct: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLVDHYLEKFKHIKLPSNLPNELLYGRAGFLWAC 180

Query: 181 LFLNKHIGQNSISNTIMRSVVGEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMH 240
           LFLNKHI Q++IS TIMRSVV EVI+ GRQLGK GKSPLMYEWHGKKYWGAAHGLAGIMH
Sbjct: 181 LFLNKHICQHTISKTIMRSVVDEVIKVGRQLGKNGKSPLMYEWHGKKYWGAAHGLAGIMH 240

Query: 241 VLMHMELKPDEVKDVKNTLRYMIKNRFPSGNYCSSEGSESDRLVHWCHGAPGVALTLIKA 300
           VLM+MELKPDEV+DVKNTLRYMIKNRFPSGN+ SSE ++SD+LVHWCHGAPGVALTLIKA
Sbjct: 241 VLMNMELKPDEVEDVKNTLRYMIKNRFPSGNFRSSEENDSDKLVHWCHGAPGVALTLIKA 300

Query: 301 AEVFGDDEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTRYLYRAKA 360
           AEVFGD EFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGD  YLYRAKA
Sbjct: 301 AEVFGDSEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDPVYLYRAKA 360

Query: 361 FACFLHQNAQKLISEGKMHSGDRPYSLFEGIGGMTYLFFDMIEPSASRFPSYEL 415
           FACFLHQNAQKLISEGKM SGDRP+S+FEGIGGM YLFFDM EPSA+RFP+YEL
Sbjct: 361 FACFLHQNAQKLISEGKMQSGDRPFSMFEGIGGMAYLFFDMNEPSAARFPAYEL 414

BLAST of Lcy12g011300 vs. NCBI nr
Match: XP_023524926.1 (lanC-like protein GCR2 [Cucurbita pepo subsp. pepo] >KAG7037398.1 LanC-like protein GCR2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 752.3 bits (1941), Expect = 2.2e-213
Identity = 367/414 (88.65%), Postives = 387/414 (93.48%), Query Frame = 0

Query: 1   MPDRFFPNRMPHFVPEAPADELAPSDSDIAEDPLTPTELLSLPHAALSDRLRQTALDLKE 60
           M DRFFP +MPHFV EAPADELAPSDSDI EDPLTP++LLSLPHAA S R  QTALDLKE
Sbjct: 1   MADRFFPIQMPHFVAEAPADELAPSDSDIDEDPLTPSKLLSLPHAARSARFIQTALDLKE 60

Query: 61  TVVRETWTLSGRYPRDYTLYTGALGTAFLALKSYLVSNNENDLKLCSEIVRACETVSRDS 120
           TVVRETWTLS RYP+DYTLYTGALGTAFLALKSY+VSNNENDLKLCSEIVRACET+SRDS
Sbjct: 61  TVVRETWTLSERYPQDYTLYTGALGTAFLALKSYVVSNNENDLKLCSEIVRACETISRDS 120

Query: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLLDHYLGNFKDIKLPSDLPNELLYGRAGFLWAC 180
           RRVSFLCGRAGVCALGAVAAKLANDGRL+DHYL  FK IKLPSDLPNELLYGRAGFLWAC
Sbjct: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLVDHYLEKFKHIKLPSDLPNELLYGRAGFLWAC 180

Query: 181 LFLNKHIGQNSISNTIMRSVVGEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMH 240
           LFLNKHI Q++IS TIMRSVV EVI+ GRQLGK  KSPLMYEWHGKKYWGAAHGLAGIMH
Sbjct: 181 LFLNKHICQHTISKTIMRSVVDEVIKVGRQLGKNRKSPLMYEWHGKKYWGAAHGLAGIMH 240

Query: 241 VLMHMELKPDEVKDVKNTLRYMIKNRFPSGNYCSSEGSESDRLVHWCHGAPGVALTLIKA 300
           VLM+MELKPDEV+DVKNTLRYMIKNRFPSGN+ SSE ++SD+LVHWCHGAPGVALTLIKA
Sbjct: 241 VLMNMELKPDEVEDVKNTLRYMIKNRFPSGNFRSSEENDSDKLVHWCHGAPGVALTLIKA 300

Query: 301 AEVFGDDEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTRYLYRAKA 360
           AEVFGD E LQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGD  YLYRAKA
Sbjct: 301 AEVFGDSELLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDPVYLYRAKA 360

Query: 361 FACFLHQNAQKLISEGKMHSGDRPYSLFEGIGGMTYLFFDMIEPSASRFPSYEL 415
           FACFLHQNAQKLISEGKM SGDRP+S+FEGIGGM YLFFDM EPSA+RFP+YEL
Sbjct: 361 FACFLHQNAQKLISEGKMQSGDRPFSMFEGIGGMAYLFFDMKEPSAARFPAYEL 414

BLAST of Lcy12g011300 vs. NCBI nr
Match: KAG6607868.1 (LanC-like protein GCR2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 752.3 bits (1941), Expect = 2.2e-213
Identity = 367/414 (88.65%), Postives = 387/414 (93.48%), Query Frame = 0

Query: 1   MPDRFFPNRMPHFVPEAPADELAPSDSDIAEDPLTPTELLSLPHAALSDRLRQTALDLKE 60
           M DRFFP +MPHFV EAPADELAPSDSDI EDPLTP++LLSLPHAA S R  QTALDLKE
Sbjct: 259 MADRFFPIQMPHFVAEAPADELAPSDSDIDEDPLTPSKLLSLPHAARSARFIQTALDLKE 318

Query: 61  TVVRETWTLSGRYPRDYTLYTGALGTAFLALKSYLVSNNENDLKLCSEIVRACETVSRDS 120
           TVVRETWTLS RYP+DYTLYTGALGTAFLALKSY+VSNNENDLKLCSEIVRACET+SRDS
Sbjct: 319 TVVRETWTLSERYPQDYTLYTGALGTAFLALKSYVVSNNENDLKLCSEIVRACETISRDS 378

Query: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLLDHYLGNFKDIKLPSDLPNELLYGRAGFLWAC 180
           RRVSFLCGRAGVCALGAVAAKLANDGRL+DHYL  FK IKLPSDLPNELLYGRAGFLWAC
Sbjct: 379 RRVSFLCGRAGVCALGAVAAKLANDGRLVDHYLEKFKHIKLPSDLPNELLYGRAGFLWAC 438

Query: 181 LFLNKHIGQNSISNTIMRSVVGEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMH 240
           LFLNKHI Q++IS TIMRSVV EVI+ GRQLGK  KSPLMYEWHGKKYWGAAHGLAGIMH
Sbjct: 439 LFLNKHICQHTISKTIMRSVVDEVIKVGRQLGKNRKSPLMYEWHGKKYWGAAHGLAGIMH 498

Query: 241 VLMHMELKPDEVKDVKNTLRYMIKNRFPSGNYCSSEGSESDRLVHWCHGAPGVALTLIKA 300
           VLM+MELKPDEV+DVKNTLRYMIKNRFPSGN+ SSE ++SD+LVHWCHGAPGVALTLIKA
Sbjct: 499 VLMNMELKPDEVEDVKNTLRYMIKNRFPSGNFRSSEENDSDKLVHWCHGAPGVALTLIKA 558

Query: 301 AEVFGDDEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTRYLYRAKA 360
           AEVFGD E LQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGD  YLYRAKA
Sbjct: 559 AEVFGDSELLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDPVYLYRAKA 618

Query: 361 FACFLHQNAQKLISEGKMHSGDRPYSLFEGIGGMTYLFFDMIEPSASRFPSYEL 415
           FACFLHQNAQKLISEGKM SGDRP+S+FEGIGGM YLFFDM EPSA+RFP+YEL
Sbjct: 619 FACFLHQNAQKLISEGKMQSGDRPFSMFEGIGGMAYLFFDMKEPSAARFPAYEL 672

BLAST of Lcy12g011300 vs. NCBI nr
Match: XP_038897162.1 (lanC-like protein GCR2 [Benincasa hispida])

HSP 1 Score: 738.4 bits (1905), Expect = 3.3e-209
Identity = 361/414 (87.20%), Postives = 382/414 (92.27%), Query Frame = 0

Query: 1   MPDRFFPNRMPHFVPEAPADELAPSDSDIAEDPLTPTELLSLPHAALSDRLRQTALDLKE 60
           M DRFFPNRMP  + E+PAD+LA SDSD A   L PTELL LP AALSDRLRQTAL+LKE
Sbjct: 1   MSDRFFPNRMPRSLAESPADQLAASDSDSANHTLIPTELLPLPDAALSDRLRQTALNLKE 60

Query: 61  TVVRETWTLSGRYPRDYTLYTGALGTAFLALKSYLVSNNENDLKLCSEIVRACETVSRDS 120
           TVVRETW L GR+ +DYTLYTGALGTAFLALKSYLVS NENDLKLCSEIV ACE+VSR+S
Sbjct: 61  TVVRETWALGGRHVQDYTLYTGALGTAFLALKSYLVSKNENDLKLCSEIVTACESVSRNS 120

Query: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLLDHYLGNFKDIKLPSDLPNELLYGRAGFLWAC 180
           RRVSF+CG+AGVCALGAVAAKLANDG L+DHYL  FKDIKLPSDLPNELLYGRAGFLWAC
Sbjct: 121 RRVSFICGQAGVCALGAVAAKLANDGSLVDHYLEKFKDIKLPSDLPNELLYGRAGFLWAC 180

Query: 181 LFLNKHIGQNSISNTIMRSVVGEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMH 240
           LFLNKH+G N+ISNTIMRSVV EVIEAGR LGKK KSPLMYEWHGKKYWGAAHGLAGIMH
Sbjct: 181 LFLNKHVGWNTISNTIMRSVVDEVIEAGRGLGKKRKSPLMYEWHGKKYWGAAHGLAGIMH 240

Query: 241 VLMHMELKPDEVKDVKNTLRYMIKNRFPSGNYCSSEGSESDRLVHWCHGAPGVALTLIKA 300
           VLM+MELKPDEV+DVKNTLRYMIKNRFPSGNY SSEG++SD LVHWCHGAPGVALTL+KA
Sbjct: 241 VLMNMELKPDEVEDVKNTLRYMIKNRFPSGNYPSSEGNKSDCLVHWCHGAPGVALTLVKA 300

Query: 301 AEVFGDDEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTRYLYRAKA 360
           AEVFGDDEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGD  +LYRAKA
Sbjct: 301 AEVFGDDEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDFIHLYRAKA 360

Query: 361 FACFLHQNAQKLISEGKMHSGDRPYSLFEGIGGMTYLFFDMIEPSASRFPSYEL 415
           FACFLHQNAQ LISEGKMHSGD PYSLFEGIGGM YLFFDMIEP A++FPSYEL
Sbjct: 361 FACFLHQNAQTLISEGKMHSGDSPYSLFEGIGGMAYLFFDMIEPYAAKFPSYEL 414

BLAST of Lcy12g011300 vs. TAIR 10
Match: AT1G52920.1 (G protein coupled receptor )

HSP 1 Score: 587.4 bits (1513), Expect = 8.9e-168
Identity = 282/414 (68.12%), Postives = 333/414 (80.43%), Query Frame = 0

Query: 1   MPDRFFPNRMPHFVPEAPADELAPSDSDIAEDPLTPTELLSLPHAALSDRLRQTALDLKE 60
           M +RFF N MP FVPE    +L+  +  + E   + T+LLSLP+ + S++L + AL +K+
Sbjct: 1   MGERFFRNEMPEFVPE----DLSGEEETVTECKDSLTKLLSLPYKSFSEKLHRYALSIKD 60

Query: 61  TVVRETWTLSGRYPRDYTLYTGALGTAFLALKSYLVSNNENDLKLCSEIVRACETVSRDS 120
            VV ETW  SG+  RDY LYTG LGTA+L  KSY V+ NE+DLKLC E V AC+  SRDS
Sbjct: 61  KVVWETWERSGKRVRDYNLYTGVLGTAYLLFKSYQVTRNEDDLKLCLENVEACDVASRDS 120

Query: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLLDHYLGNFKDIKLPSDLPNELLYGRAGFLWAC 180
            RV+F+CG AGVCALGAVAAK   D +L D YL  F+ I+LPSDLP ELLYGRAG+LWAC
Sbjct: 121 ERVTFICGYAGVCALGAVAAKCLGDDQLYDRYLARFRGIRLPSDLPYELLYGRAGYLWAC 180

Query: 181 LFLNKHIGQNSISNTIMRSVVGEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMH 240
           LFLNKHIGQ SIS+  MRSVV E+  AGRQLG KG  PLMYEWHGK+YWGAAHGLAGIM+
Sbjct: 181 LFLNKHIGQESISSERMRSVVEEIFRAGRQLGNKGTCPLMYEWHGKRYWGAAHGLAGIMN 240

Query: 241 VLMHMELKPDEVKDVKNTLRYMIKNRFPSGNYCSSEGSESDRLVHWCHGAPGVALTLIKA 300
           VLMH EL+PDE+KDVK TL YMI+NRFPSGNY SSEGS+SDRLVHWCHGAPGVALTL+KA
Sbjct: 241 VLMHTELEPDEIKDVKGTLSYMIQNRFPSGNYLSSEGSKSDRLVHWCHGAPGVALTLVKA 300

Query: 301 AEVFGDDEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTRYLYRAKA 360
           A+V+   EF++AA++AGEVVW+RGLLKRVGICHGISGNTYVFLSLYRLT + +YLYRAKA
Sbjct: 301 AQVYNTKEFVEAAMEAGEVVWSRGLLKRVGICHGISGNTYVFLSLYRLTRNPKYLYRAKA 360

Query: 361 FACFLHQNAQKLISEGKMHSGDRPYSLFEGIGGMTYLFFDMIEPSASRFPSYEL 415
           FA FL   ++KLISEG+MH GDRP+SLFEGIGGM Y+  DM +P+ + FP YEL
Sbjct: 361 FASFLLDKSEKLISEGQMHGGDRPFSLFEGIGGMAYMLLDMNDPTQALFPGYEL 410

BLAST of Lcy12g011300 vs. TAIR 10
Match: AT2G20770.1 (GCR2-like 2 )

HSP 1 Score: 542.7 bits (1397), Expect = 2.5e-154
Identity = 262/415 (63.13%), Postives = 321/415 (77.35%), Query Frame = 0

Query: 1   MPDRFFPNRMPHFVPEAPADELAPSDSDIAEDPLTPTELLSLPHAALSDRLRQTALDLKE 60
           M  RFF N MP FV E   + ++  D        T   LL++P+++LS +L+++ALDLKE
Sbjct: 1   MAGRFFDNVMPDFVKE--KESVSGGD--------TLRNLLAMPYSSLSQQLKRSALDLKE 60

Query: 61  TVVRETWTLSGRYPRDYTLYTGALGTAFLALKSYLVSNNENDLKLCSEIVRACETVSRDS 120
           TVV ETW  SG+   D+TLY+G LG AFL  ++Y V+ N NDL LC EIV+AC+T S  S
Sbjct: 61  TVVIETWGFSGQTVEDFTLYSGTLGAAFLLFRAYQVTGNANDLSLCLEIVKACDTASASS 120

Query: 121 RRVSFLCGRAGVCALGAVAAKLANDGRLLDHYLGNFKDIKLPSDLPNELLYGRAGFLWAC 180
             V+FLCGRAGVC LGAVAAKL+ +  LL++YLG F+ I+L SDLPNELLYGR G+LWAC
Sbjct: 121 GDVTFLCGRAGVCGLGAVAAKLSGEEDLLNYYLGQFRLIRLSSDLPNELLYGRVGYLWAC 180

Query: 181 LFLNKHIGQNSISNTIMRSVVGEVIEAGRQLGKKGKSPLMYEWHGKKYWGAAHGLAGIMH 240
           LF+NK+IG+ ++S+  +R V  E+I+ GR + KKG SPLM+EW+GK+YWGAAHGLAGIMH
Sbjct: 181 LFINKYIGKETLSSDTIREVAQEIIKEGRSMAKKGSSPLMFEWYGKRYWGAAHGLAGIMH 240

Query: 241 VLMHMELKPDEVKDVKNTLRYMIKNRFPSGNY-CSSEGSESDRLVHWCHGAPGVALTLIK 300
           VLM ++LKPDE +DVK TL+YMIKNRFPSGNY  S E  + D LVHWCHGAPG+ALTL K
Sbjct: 241 VLMDVQLKPDEAEDVKGTLKYMIKNRFPSGNYPASEEDKKKDILVHWCHGAPGIALTLGK 300

Query: 301 AAEVFGDDEFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTRYLYRAK 360
           AAEVFG+ EFL+A+  A EVVWNRGLLKRVGICHGISGN YVFL+LYR TG + YLYRAK
Sbjct: 301 AAEVFGEREFLEASAAAAEVVWNRGLLKRVGICHGISGNAYVFLALYRATGRSEYLYRAK 360

Query: 361 AFACFLHQNAQKLISEGKMHSGDRPYSLFEGIGGMTYLFFDMIEPSASRFPSYEL 415
           AFA FL     KL+S+G+MH GD PYSLFEG+ GM YLF DM++PS +RFP YEL
Sbjct: 361 AFASFLLDRGPKLLSKGEMHGGDSPYSLFEGVAGMAYLFLDMVDPSEARFPGYEL 405

BLAST of Lcy12g011300 vs. TAIR 10
Match: AT5G65280.1 (GCR2-like 1 )

HSP 1 Score: 349.7 bits (896), Expect = 3.1e-96
Identity = 187/408 (45.83%), Postives = 261/408 (63.97%), Query Frame = 0

Query: 29  IAEDPLTPTELLSLPHAALSDRLRQTALDLKETVVRETW------TLSGRYP-RDYTLYT 88
           +  +P  PT  +SLP    ++   + A  LK  VV  TW        SG  P  D T+YT
Sbjct: 33  LLSEPSAPT--ISLP----TESFLRAATLLKNQVVEATWKGGVEALASGSGPVLDPTVYT 92

Query: 89  GALGTAFLALKSYLVSNNENDLKLCSEIVRACETVSR-DSRRVSFLCGRAGVCALGAVAA 148
           G LGTAF  LKSY V+ N  DL  C+EI+  C  V+R  +R V+FLCGR GVC LGA+ A
Sbjct: 93  GLLGTAFTCLKSYEVTRNHQDLLTCAEIIDTCANVARATTRHVTFLCGRGGVCTLGAIVA 152

Query: 149 KLANDGRLLDHYLGNFKDIKLPSDLP-----------NELLYGRAGFLWACLFLNKHIGQ 208
               D    D +LG F ++    +LP            +LLYGRAGFLWA LFLN+++GQ
Sbjct: 153 NYRGDQSKRDFFLGLFLELAEERELPAGPEEGGFGMSYDLLYGRAGFLWAALFLNRYLGQ 212

Query: 209 NSISNTIMRSVVGEVIEAGRQLGKKGKS-PLMYEWHGKKYWGAAHGLAGIMHVLMHMELK 268
            ++ + ++  +V  ++  GR      ++ PL+Y +HG ++WGAA+GLAGI++VL+H  L 
Sbjct: 213 GTVPDHLLSPIVAAILAGGRVGAADHEACPLLYRFHGTRFWGAANGLAGILYVLLHFPLS 272

Query: 269 PDEVKDVKNTLRYMIKNRFP-SGNYCSSEGSESDRLVHWCHGAPGVALTLIKAAEVFGDD 328
            ++VKDV+ TLRYM+ NRFP SGNY  SEG+  D+LV W HGA G+A+TL KA++VF  +
Sbjct: 273 EEDVKDVQGTLRYMMSNRFPNSGNYPCSEGNPRDKLVQWAHGATGMAITLAKASQVFPKE 332

Query: 329 -EFLQAALDAGEVVWNRGLLKRVGICHGISGNTYVFLSLYRLTGDTRYLYRAKAFACFLH 388
            +F +AA++AGEVVW  GL+K+VG+  G++GN Y FLSLYRLTGD  Y  RAKAFA +L 
Sbjct: 333 RDFREAAIEAGEVVWKSGLVKKVGLADGVAGNAYAFLSLYRLTGDVVYEERAKAFASYLC 392

Query: 389 QNAQKLISEGKMHSGDRPYSLFEGIGGMTYLFFDMIEPSASRFPSYEL 415
           ++A +L++     + +  YSLF G+ G   L+FD++ P  S+FP YE+
Sbjct: 393 RDAIELVNMTSQET-EHDYSLFRGLAGPVCLWFDLVSPVDSKFPGYEI 433