Lcy12g006430 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy12g006430
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	ERAD-associated E3 ubiquitin-protein ligase HRD1-like
Location	Chr12: 27869775 .. 27870461 (-)
RNA-Seq Expression	Lcy12g006430
Synteny	Lcy12g006430

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGGTTGAAGTCGAAACACCGACTTTGAAACCCCAACAAGTTCTCATAAACCCGCTGTCCATTGAAGATATCATACTCAAATTCAAACTCCAAATCGTATGCAATCACGTCCTAGTAGAAGAAAACGGGGATACATTGTTGGTGAGGCAACATGAATCTCGAAAACTTCCCTTTGTAGATCATCAACTTCCCGTCCAAAACTTCGACCATTCCGACGACGGAGCCCTACAAGACTTCTTGTCATTGAAGTTCACAACATACGGTACTAACCGTGTTTTCGACGTCAAGACGATCACCGACGAGATAATAAACAAGTGGACTATGATCAAAGACGAAGTCGCGAATTCGAGTAGGTTTGGAAAGGTTTTTCCTCTTATGGTTACGCTAAAATTGTGGAACGTCGTGACGCGTCGTGAGAGACCGTCATTCATGAATGTGAGTGAATATAGAATGGTTCCGACGTGCGATCACGCTATCGAGATGTTAACGAGAGCGAAGATCGAGGAGGTCGAAAGCGATCAAAGATGTGTAATTTGCTTGGAGGATATAGAAAAAGAGGGAGAATGTGCATTGCTGTTACTGAAGATGCCTTGCTTGCATGTGTTTCATGAAGAATGCATAATAAGATGGCTGAAGACTAGTCATTCCTGTCCTATTTGTAGATTTAACATGCCTACAGAATGA

mRNA sequence

ATGTCGGTTGAAGTCGAAACACCGACTTTGAAACCCCAACAAGTTCTCATAAACCCGCTGTCCATTGAAGATATCATACTCAAATTCAAACTCCAAATCGTATGCAATCACGTCCTAGTAGAAGAAAACGGGGATACATTGTTGGTGAGGCAACATGAATCTCGAAAACTTCCCTTTGTAGATCATCAACTTCCCGTCCAAAACTTCGACCATTCCGACGACGGAGCCCTACAAGACTTCTTGTCATTGAAGTTCACAACATACGGTACTAACCGTGTTTTCGACGTCAAGACGATCACCGACGAGATAATAAACAAGTGGACTATGATCAAAGACGAAGTCGCGAATTCGAGTAGGTTTGGAAAGGTTTTTCCTCTTATGGTTACGCTAAAATTGTGGAACGTCGTGACGCGTCGTGAGAGACCGTCATTCATGAATGTGAGTGAATATAGAATGGTTCCGACGTGCGATCACGCTATCGAGATGTTAACGAGAGCGAAGATCGAGGAGGTCGAAAGCGATCAAAGATGTGTAATTTGCTTGGAGGATATAGAAAAAGAGGGAGAATGTGCATTGCTGTTACTGAAGATGCCTTGCTTGCATGTGTTTCATGAAGAATGCATAATAAGATGGCTGAAGACTAGTCATTCCTGTCCTATTTGTAGATTTAACATGCCTACAGAATGA

Coding sequence (CDS)

ATGTCGGTTGAAGTCGAAACACCGACTTTGAAACCCCAACAAGTTCTCATAAACCCGCTGTCCATTGAAGATATCATACTCAAATTCAAACTCCAAATCGTATGCAATCACGTCCTAGTAGAAGAAAACGGGGATACATTGTTGGTGAGGCAACATGAATCTCGAAAACTTCCCTTTGTAGATCATCAACTTCCCGTCCAAAACTTCGACCATTCCGACGACGGAGCCCTACAAGACTTCTTGTCATTGAAGTTCACAACATACGGTACTAACCGTGTTTTCGACGTCAAGACGATCACCGACGAGATAATAAACAAGTGGACTATGATCAAAGACGAAGTCGCGAATTCGAGTAGGTTTGGAAAGGTTTTTCCTCTTATGGTTACGCTAAAATTGTGGAACGTCGTGACGCGTCGTGAGAGACCGTCATTCATGAATGTGAGTGAATATAGAATGGTTCCGACGTGCGATCACGCTATCGAGATGTTAACGAGAGCGAAGATCGAGGAGGTCGAAAGCGATCAAAGATGTGTAATTTGCTTGGAGGATATAGAAAAAGAGGGAGAATGTGCATTGCTGTTACTGAAGATGCCTTGCTTGCATGTGTTTCATGAAGAATGCATAATAAGATGGCTGAAGACTAGTCATTCCTGTCCTATTTGTAGATTTAACATGCCTACAGAATGA

Protein sequence

MSVEVETPTLKPQQVLINPLSIEDIILKFKLQIVCNHVLVEENGDTLLVRQHESRKLPFVDHQLPVQNFDHSDDGALQDFLSLKFTTYGTNRVFDVKTITDEIINKWTMIKDEVANSSRFGKVFPLMVTLKLWNVVTRRERPSFMNVSEYRMVPTCDHAIEMLTRAKIEEVESDQRCVICLEDIEKEGECALLLLKMPCLHVFHEECIIRWLKTSHSCPICRFNMPTE

Homology

[Close]

BLAST of Lcy12g006430 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 9.7e-12
Identity = 32/77 (41.56%), Postives = 46/77 (59.74%), Query Frame = 0


Query: 154 PTCDHAIEMLTRAKIEE--VESDQRCVICLEDIEKEGECALLLLKMPCLHVFHEECIIRW 213
           P    AIE L R +I +  + S+  C +C E  E E E      +MPC H+FH++CI+ W
Sbjct: 174 PASKSAIESLPRVEISDCHIGSEANCAVCTEIFETETEAR----EMPCKHLFHDDCIVPW 233

Query: 214 LKTSHSCPICRFNMPTE 229
           L   +SCP+CRF +P+E
Sbjct: 234 LSIRNSCPVCRFELPSE 246

[Close]

BLAST of Lcy12g006430 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 3.7e-11
Identity = 33/75 (44.00%), Postives = 44/75 (58.67%), Query Frame = 0


Query: 154 PTCDHAIEMLTRAKIEEVESDQRCVICLEDIEKEGECALLLLKMPCLHVFHEECIIRWLK 213
           P    A+E L   KIEEV S   C +CL+D+E   +      +MPC H FH  CI+ WL+
Sbjct: 179 PAKKEAVEALPTVKIEEVVS---CSVCLDDLEVGSQAK----QMPCEHKFHSSCILPWLE 238

Query: 214 TSHSCPICRFNMPTE 229
              SCP+CRF +P+E
Sbjct: 239 LHSSCPVCRFELPSE 246

[Close]

BLAST of Lcy12g006430 vs. ExPASy Swiss-Prot
Match: Q9CY62 (E3 ubiquitin-protein ligase RNF181 OS=Mus musculus OX=10090 GN=Rnf181 PE=1 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 8.2e-11
Identity = 28/75 (37.33%), Postives = 44/75 (58.67%), Query Frame = 0


Query: 154 PTCDHAIEMLTRAKIEEVESDQRCVICLEDIEKEGECALLLLKMPCLHVFHEECIIRWLK 213
           P     +E L R  I   ++D +C +CL + E E      +++MPC H+FH  CI+ WL 
Sbjct: 65  PAAKAVVESLPRTVISSAKADLKCPVCLLEFEAEE----TVIEMPCHHLFHSNCILPWLS 124

Query: 214 TSHSCPICRFNMPTE 229
            ++SCP+CR  +PT+
Sbjct: 125 KTNSCPLCRHELPTD 135

[Close]

BLAST of Lcy12g006430 vs. ExPASy Swiss-Prot
Match: Q3T0W3 (E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.8e-10
Identity = 30/75 (40.00%), Postives = 47/75 (62.67%), Query Frame = 0


Query: 154 PTCDHAIEMLTRAKIEEVESDQRCVICLEDIEKEGECALLLLKMPCLHVFHEECIIRWLK 213
           P    A+E L R  I   +++ +C +CL + E+E E A   ++MPC H+FH  CI+ WL 
Sbjct: 53  PAAKTAVENLPRTVIRGSQAELKCPVCLLEFEEE-ETA---IEMPCHHLFHSNCILPWLS 112

Query: 214 TSHSCPICRFNMPTE 229
            ++SCP+CR  +PT+
Sbjct: 113 KTNSCPLCRHELPTD 123

[Close]

BLAST of Lcy12g006430 vs. ExPASy Swiss-Prot
Match: Q9P0P0 (E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.8e-10
Identity = 29/75 (38.67%), Postives = 47/75 (62.67%), Query Frame = 0


Query: 154 PTCDHAIEMLTRAKIEEVESDQRCVICLEDIEKEGECALLLLKMPCLHVFHEECIIRWLK 213
           P     +E L R  I   +++ +C +CL + E+E E A   ++MPC H+FH  CI+ WL 
Sbjct: 53  PAAKTVVENLPRTVIRGSQAELKCPVCLLEFEEE-ETA---IEMPCHHLFHSSCILPWLS 112

Query: 214 TSHSCPICRFNMPTE 229
            ++SCP+CR+ +PT+
Sbjct: 113 KTNSCPLCRYELPTD 123

[Close]

BLAST of Lcy12g006430 vs. ExPASy TrEMBL
Match: A0A6J1BQW1 (uncharacterized protein LOC111004784 OS=Momordica charantia OX=3673 GN=LOC111004784 PE=4 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 3.8e-51
Identity = 126/246 (51.22%), Postives = 157/246 (63.82%), Query Frame = 0


Query: 1   MSVEVETPTLKPQQ-VLINPLSIEDIILKFKLQIVCNHVLVEENGD---TLLVRQHESRK 60
           M+VEV+ PT KP+Q     P  +E+I LKF   + C+H LV  N +   T L R+HES K
Sbjct: 13  MTVEVQPPTPKPEQPPQTQPDPVEEIRLKFVAVVECSHFLVSTNNNFRTTTLTRRHESEK 72

Query: 61  LPFVDHQLPVQNFDHSDDGALQDFLSLKFTTYGTNRVFDVKTITDEIINKWTMIK-DEVA 120
           +P V HQ+P+Q  DH    +L  FLS KF  Y  + VF++ TITD+II  W  IK  +  
Sbjct: 73  VPLVVHQIPIQILDHGPQ-SLHQFLSSKFPAYQNDTVFNIHTITDKIIATWAEIKQQQEQ 132

Query: 121 NSSRF-GKVFPLMVTLKLWN-VVTRRERPSFMNVSEYRMVPTCDHAI-EMLTRAKIEEVE 180
           N S F GKV PL VTL+LWN VVTR E P    + +  M+PT D A+ EML R K+E+  
Sbjct: 133 NLSVFVGKVLPLTVTLRLWNVVVTRHETP----MEDRFMIPTSDFAVEEMLKRVKVEQQF 192

Query: 181 SD----QRCVICLEDI------EKEGECALLLLKMPCLHVFHEECIIRWLKTSHSCPICR 229
            D    + CVICLEDI      E+ GE  LLLL+MPCLHVFHEECI +WLK+SH CPICR
Sbjct: 193 DDDDQIRSCVICLEDISVNMEKEENGEVILLLLQMPCLHVFHEECIKKWLKSSHYCPICR 252

[Close]

BLAST of Lcy12g006430 vs. ExPASy TrEMBL
Match: A0A6J1HDB7 (probable E3 ubiquitin-protein ligase ZFP1 OS=Cucurbita moschata OX=3662 GN=LOC111461780 PE=4 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 1.7e-35
Identity = 94/229 (41.05%), Postives = 133/229 (58.08%), Query Frame = 0


Query: 1   MSVEVETPTLKPQQVLINPLSIEDIILKFKLQIVCNHVLVEENGDTLLVRQHESRKLPFV 60
           +SV+V       QQ L  P +++ I LKF++++ C H+ V  N   +    H  R+LPF 
Sbjct: 14  LSVQVTASPPNLQQPL-PPQTLQQISLKFRVEVKCRHIQVANNNTVISFTAHRYRRLPFP 73

Query: 61  DHQLPVQNFDHSDDGALQDFLSLKFTTYGTNRVFDVKTITDEIINKWTMIKDEVANSSRF 120
             QLP+   D  ++ ALQ+ LS+ F  Y  + + DVK + DEI  KW  I+    + + F
Sbjct: 74  ICQLPLAVIDRDNNPALQNCLSVHFDDY--SELVDVKKVADEIKRKWIEIRVR-EDLNAF 133

Query: 121 GKVFPLMVTLKLWNVVTRRERPSFMNVSEYRMVPTCDHA--IE-MLTRAKIEEVESDQRC 180
           G+VF L VTLK+WNV        F N      V +CD +  IE ML R + EE+ESD  C
Sbjct: 134 GRVFRLTVTLKVWNV------GMFYNGRVEDGVMSCDRSRGIEMMLMRVRFEEIESDDVC 193

Query: 181 VICLEDIEKEGECALLLLKMPCLHVFHEECIIRWLKTSHSCPICRFNMP 227
           VICLE++E+  +    L+ MPC HVFH  CI++WL  ++SCP+CRF MP
Sbjct: 194 VICLEELERRKKS---LVGMPCFHVFHRNCIVKWLADTNSCPVCRFQMP 229

[Close]

BLAST of Lcy12g006430 vs. ExPASy TrEMBL
Match: A0A5A7SNF1 (RING finger protein 141-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447G00770 PE=4 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 1.4e-32
Identity = 89/231 (38.53%), Postives = 132/231 (57.14%), Query Frame = 0


Query: 2   SVEVETPTLKPQQVLINPLSIEDIILKFKLQIVCNHVLVEENGDTLLVRQHESRKLPFVD 61
           +VE++      +++  +P  IE I L F L+  CN   V ENG+  LV + E+ K+P  D
Sbjct: 12  AVEIDIDLPMSRRIRKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVEEQEASKVPLCD 71

Query: 62  HQLPVQNFDHSDDGALQDFLSLKFTTYGTNRVFDVKTITDEIINKWTMIKDEVANSSRFG 121
           HQ PV+ F + DD +L  +L   F  Y      D + +   +I +W  I+D++ NS    
Sbjct: 72  HQFPVEIF-NGDDNSLFQYLCCSFENYEPK--IDAEVVATALIEQWIGIRDQL-NSRSCH 131

Query: 122 KVFPLMVTLKLWNVVTR---RERPSFMNVSEYRMVPTCDHAI-EMLTRAKIEEVESDQRC 181
           KVF + V L + +VV +    E  S     ++R VP CD A+ +ML R ++ + E ++ C
Sbjct: 132 KVFSMTVNLTITDVVVQYVIGEDGSSFGGEDFRAVPACDKALKKMLKRLEVRD-EDERIC 191

Query: 182 VICLEDI--EKEGECALLLLKMPCLHVFHEECIIRWLKTSHSCPICRFNMP 227
           +ICL+++  +K G C    L+MPCLHVFH +CI  WLK SH CPICRF MP
Sbjct: 192 IICLDEVRKKKNGRCG---LEMPCLHVFHGKCIENWLKNSHCCPICRFQMP 234

[Close]

BLAST of Lcy12g006430 vs. ExPASy TrEMBL
Match: A0A1S4E0Q9 (RING finger protein 141-like OS=Cucumis melo OX=3656 GN=LOC103495859 PE=4 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 1.4e-32
Identity = 89/231 (38.53%), Postives = 132/231 (57.14%), Query Frame = 0


Query: 2   SVEVETPTLKPQQVLINPLSIEDIILKFKLQIVCNHVLVEENGDTLLVRQHESRKLPFVD 61
           +VE++      +++  +P  IE I L F L+  CN   V ENG+  LV + E+ K+P  D
Sbjct: 12  AVEIDIDLPMSRRIRKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVEEQEASKVPLCD 71

Query: 62  HQLPVQNFDHSDDGALQDFLSLKFTTYGTNRVFDVKTITDEIINKWTMIKDEVANSSRFG 121
           HQ PV+ F + DD +L  +L   F  Y      D + +   +I +W  I+D++ NS    
Sbjct: 72  HQFPVEIF-NGDDNSLFQYLCCSFENYEPK--IDAEVVATALIEQWIGIRDQL-NSRSCH 131

Query: 122 KVFPLMVTLKLWNVVTR---RERPSFMNVSEYRMVPTCDHAI-EMLTRAKIEEVESDQRC 181
           KVF + V L + +VV +    E  S     ++R VP CD A+ +ML R ++ + E ++ C
Sbjct: 132 KVFSMTVNLTITDVVVQYVIGEDGSSFGGEDFRAVPACDKALKKMLKRLEVRD-EDERIC 191

Query: 182 VICLEDI--EKEGECALLLLKMPCLHVFHEECIIRWLKTSHSCPICRFNMP 227
           +ICL+++  +K G C    L+MPCLHVFH +CI  WLK SH CPICRF MP
Sbjct: 192 IICLDEVRKKKNGRCG---LEMPCLHVFHGKCIENWLKNSHCCPICRFQMP 234

[Close]

BLAST of Lcy12g006430 vs. ExPASy TrEMBL
Match: A0A5D3C4K6 (Putative zinc finger protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001670 PE=4 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 1.1e-31
Identity = 93/249 (37.35%), Postives = 139/249 (55.82%), Query Frame = 0


Query: 1   MSVEVETPTLKPQQV-----LINPLSIEDIILKFKLQIVCNHVLVEENGD---TLLVRQH 60
           MS+ +  P  + Q +       +P  +E I LKFK+Q+ C H  V E G+   T LVR+H
Sbjct: 19  MSLRITDPNPRHQPLNQIDQTSSPTLVEQICLKFKVQLKCQHFQVWEGGNGHSTTLVREH 78

Query: 61  ESRKLPFVDHQLPVQNFDHSDDGALQDFLSLKFTTYGTNRVFDVKTITDEIINKWTM--- 120
              +  F   QLP+  F H D   L+  L  +F  YG   + +++ + +EII  W     
Sbjct: 79  IRSQHSFATFQLPISIFKHGDK-TLKRILFREFKMYGD--IINIELLANEIIKHWVRKVE 138

Query: 121 -IKDEVANSS-RFGKVFPLMVTLKLW-NVVTRRERPSFMNVSEYRMVPTCDHAIE-MLTR 180
             +D+  NS+  F K++PL + ++L  + + R  +P    + +  MVPT D A+E ML R
Sbjct: 139 EEEDQENNSNVLFKKIYPLEIMIRLLVSKMIRAVQPRLAMIDQPLMVPTSDSAVESMLKR 198

Query: 181 AKIEEV-----ESDQRCVICLEDIEKEGE-CALLLLKMPCLHVFHEECIIRWLKTSHSCP 229
            + EE+     +    CV+CLE+I KE +     +L+MPCLH+FHEECI +WLKTSH CP
Sbjct: 199 VENEEIMKSGDDESINCVVCLEEISKEEKGSETTVLQMPCLHMFHEECIRKWLKTSHYCP 258

[Close]

BLAST of Lcy12g006430 vs. NCBI nr
Match: XP_022131659.1 (uncharacterized protein LOC111004784 [Momordica charantia])

HSP 1 Score: 211.5 bits (537), Expect = 7.8e-51
Identity = 126/246 (51.22%), Postives = 157/246 (63.82%), Query Frame = 0


Query: 1   MSVEVETPTLKPQQ-VLINPLSIEDIILKFKLQIVCNHVLVEENGD---TLLVRQHESRK 60
           M+VEV+ PT KP+Q     P  +E+I LKF   + C+H LV  N +   T L R+HES K
Sbjct: 13  MTVEVQPPTPKPEQPPQTQPDPVEEIRLKFVAVVECSHFLVSTNNNFRTTTLTRRHESEK 72

Query: 61  LPFVDHQLPVQNFDHSDDGALQDFLSLKFTTYGTNRVFDVKTITDEIINKWTMIK-DEVA 120
           +P V HQ+P+Q  DH    +L  FLS KF  Y  + VF++ TITD+II  W  IK  +  
Sbjct: 73  VPLVVHQIPIQILDHGPQ-SLHQFLSSKFPAYQNDTVFNIHTITDKIIATWAEIKQQQEQ 132

Query: 121 NSSRF-GKVFPLMVTLKLWN-VVTRRERPSFMNVSEYRMVPTCDHAI-EMLTRAKIEEVE 180
           N S F GKV PL VTL+LWN VVTR E P    + +  M+PT D A+ EML R K+E+  
Sbjct: 133 NLSVFVGKVLPLTVTLRLWNVVVTRHETP----MEDRFMIPTSDFAVEEMLKRVKVEQQF 192

Query: 181 SD----QRCVICLEDI------EKEGECALLLLKMPCLHVFHEECIIRWLKTSHSCPICR 229
            D    + CVICLEDI      E+ GE  LLLL+MPCLHVFHEECI +WLK+SH CPICR
Sbjct: 193 DDDDQIRSCVICLEDISVNMEKEENGEVILLLLQMPCLHVFHEECIKKWLKSSHYCPICR 252

[Close]

BLAST of Lcy12g006430 vs. NCBI nr
Match: XP_022961194.1 (probable E3 ubiquitin-protein ligase ZFP1 [Cucurbita moschata])

HSP 1 Score: 159.5 bits (402), Expect = 3.5e-35
Identity = 94/229 (41.05%), Postives = 133/229 (58.08%), Query Frame = 0


Query: 1   MSVEVETPTLKPQQVLINPLSIEDIILKFKLQIVCNHVLVEENGDTLLVRQHESRKLPFV 60
           +SV+V       QQ L  P +++ I LKF++++ C H+ V  N   +    H  R+LPF 
Sbjct: 14  LSVQVTASPPNLQQPL-PPQTLQQISLKFRVEVKCRHIQVANNNTVISFTAHRYRRLPFP 73

Query: 61  DHQLPVQNFDHSDDGALQDFLSLKFTTYGTNRVFDVKTITDEIINKWTMIKDEVANSSRF 120
             QLP+   D  ++ ALQ+ LS+ F  Y  + + DVK + DEI  KW  I+    + + F
Sbjct: 74  ICQLPLAVIDRDNNPALQNCLSVHFDDY--SELVDVKKVADEIKRKWIEIRVR-EDLNAF 133

Query: 121 GKVFPLMVTLKLWNVVTRRERPSFMNVSEYRMVPTCDHA--IE-MLTRAKIEEVESDQRC 180
           G+VF L VTLK+WNV        F N      V +CD +  IE ML R + EE+ESD  C
Sbjct: 134 GRVFRLTVTLKVWNV------GMFYNGRVEDGVMSCDRSRGIEMMLMRVRFEEIESDDVC 193

Query: 181 VICLEDIEKEGECALLLLKMPCLHVFHEECIIRWLKTSHSCPICRFNMP 227
           VICLE++E+  +    L+ MPC HVFH  CI++WL  ++SCP+CRF MP
Sbjct: 194 VICLEELERRKKS---LVGMPCFHVFHRNCIVKWLADTNSCPVCRFQMP 229

[Close]

BLAST of Lcy12g006430 vs. NCBI nr
Match: XP_016901804.1 (PREDICTED: RING finger protein 141-like [Cucumis melo] >KAA0025781.1 RING finger protein 141-like [Cucumis melo var. makuwa] >TYK09674.1 RING finger protein 141-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 149.8 bits (377), Expect = 2.8e-32
Identity = 89/231 (38.53%), Postives = 132/231 (57.14%), Query Frame = 0


Query: 2   SVEVETPTLKPQQVLINPLSIEDIILKFKLQIVCNHVLVEENGDTLLVRQHESRKLPFVD 61
           +VE++      +++  +P  IE I L F L+  CN   V ENG+  LV + E+ K+P  D
Sbjct: 12  AVEIDIDLPMSRRIRKHPSPIELIGLLFTLRRRCNRFQVLENGERQLVEEQEASKVPLCD 71

Query: 62  HQLPVQNFDHSDDGALQDFLSLKFTTYGTNRVFDVKTITDEIINKWTMIKDEVANSSRFG 121
           HQ PV+ F + DD +L  +L   F  Y      D + +   +I +W  I+D++ NS    
Sbjct: 72  HQFPVEIF-NGDDNSLFQYLCCSFENYEPK--IDAEVVATALIEQWIGIRDQL-NSRSCH 131

Query: 122 KVFPLMVTLKLWNVVTR---RERPSFMNVSEYRMVPTCDHAI-EMLTRAKIEEVESDQRC 181
           KVF + V L + +VV +    E  S     ++R VP CD A+ +ML R ++ + E ++ C
Sbjct: 132 KVFSMTVNLTITDVVVQYVIGEDGSSFGGEDFRAVPACDKALKKMLKRLEVRD-EDERIC 191

Query: 182 VICLEDI--EKEGECALLLLKMPCLHVFHEECIIRWLKTSHSCPICRFNMP 227
           +ICL+++  +K G C    L+MPCLHVFH +CI  WLK SH CPICRF MP
Sbjct: 192 IICLDEVRKKKNGRCG---LEMPCLHVFHGKCIENWLKNSHCCPICRFQMP 234

[Close]

BLAST of Lcy12g006430 vs. NCBI nr
Match: XP_023511497.1 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 147.9 bits (372), Expect = 1.1e-31
Identity = 87/227 (38.33%), Postives = 122/227 (53.74%), Query Frame = 0


Query: 3   VEVETPTLKPQQVLINPLSIEDIILKFKLQIVCNHVLVEENGDTLLVRQHESRKLPFVDH 62
           VEV T    P ++  +P  IE I L F L+   +H  V ENG+  L++Q E+ K+P  D+
Sbjct: 13  VEVLTDLPTPSRIPKHPSRIEHIGLIFTLRRRRSHFEVSENGERRLIQQRETLKIPLCDN 72

Query: 63  QLPVQNFDHSDDGALQDFLSLKFTTYGTNRVFDVKTITDEIINKWTMIKDEVANSSRFGK 122
           Q   + F+  DD     F SL  +        D + + D +I +W  I+DE ANS    +
Sbjct: 73  QFRAEIFNRDDDSL---FHSLSCSFQNFKPEIDAEPVADALIERWIEIRDE-ANSRSSDR 132

Query: 123 VFPLMVTLKLWNVVTRRERPSFMNVSEYRMVPTCDHAIEMLTRAKIEEVESDQRCVICLE 182
           VFP+ V L +W++V R E           MVP C  A+  + R    E E D  CVICL+
Sbjct: 133 VFPMTVNLTIWHIVARYEIGVGSGGDGCGMVPACGGAVAKMLRRLKREDEVDGICVICLD 192

Query: 183 DIE-KEGECALLLLKMPCLHVFHEECIIRWLKTSHSCPICRFNMPTE 229
           +++ K+ +     L+MPCLHVFH  CI  WLK SH CPICRF +P +
Sbjct: 193 ELKNKKKKNGRFTLQMPCLHVFHGNCIEDWLKNSHYCPICRFEVPIQ 235

[Close]

BLAST of Lcy12g006430 vs. NCBI nr
Match: XP_023516070.1 (E3 ubiquitin-protein ligase RNF165-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 147.1 bits (370), Expect = 1.8e-31
Identity = 88/226 (38.94%), Postives = 127/226 (56.19%), Query Frame = 0


Query: 1   MSVEVETPTLKPQQVLINPLSIEDIILKFKLQIVCNHVLVEENGDTLLVRQHESRKLPFV 60
           +SV+V       QQ L  P +++ I LKF++++ C H+ V  N   +    H  R+LPF 
Sbjct: 14  LSVQVRASPPDLQQPL-PPQTLQQISLKFRVEVKCRHIQVANNNTVISFTAHRYRRLPFP 73

Query: 61  DHQLPVQNFDHSDDGALQDFLSLKFTTYGTNRVFDVKTITDEIINKWTMIKDEVANSSRF 120
              LP+   +     ALQ+ LS+ F  Y  + + D   + DEII KW  I+ E  + + F
Sbjct: 74  ICHLPLALVERDHIPALQNRLSVHFADY--SELVDPTKVADEIIRKWIEIR-EREDLNAF 133

Query: 121 GKVFPLMVTLKLWNVVTRRERPSFMNVSEYRMVPTCDHAIEM-LTRAKIEEVESDQRCVI 180
           G+VF L VTLK+WNV    +R     V +  M       IEM L R + EE+ESD  CVI
Sbjct: 134 GRVFRLTVTLKVWNVGMFYDR----RVEDEVMSCDRSRGIEMILMRVRFEEIESDDVCVI 193

Query: 181 CLEDIEKEGECALLLLKMPCLHVFHEECIIRWLKTSHSCPICRFNM 226
           CLE++++  +    L+ MPC HVFH  CI++WL  ++SCP+CRF M
Sbjct: 194 CLEELKRRKKS---LVGMPCFHVFHRNCIVKWLADTNSCPVCRFQM 228

[Close]

BLAST of Lcy12g006430 vs. TAIR 10
Match: AT5G59550.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 72.0 bits (175), Expect = 6.9e-13
Identity = 32/77 (41.56%), Postives = 46/77 (59.74%), Query Frame = 0


Query: 154 PTCDHAIEMLTRAKIEE--VESDQRCVICLEDIEKEGECALLLLKMPCLHVFHEECIIRW 213
           P    AIE L R +I +  + S+  C +C E  E E E      +MPC H+FH++CI+ W
Sbjct: 174 PASKSAIESLPRVEISDCHIGSEANCAVCTEIFETETEAR----EMPCKHLFHDDCIVPW 233

Query: 214 LKTSHSCPICRFNMPTE 229
           L   +SCP+CRF +P+E
Sbjct: 234 LSIRNSCPVCRFELPSE 246

[Close]

BLAST of Lcy12g006430 vs. TAIR 10
Match: AT3G13228.1 (RING/U-box superfamily protein )

HSP 1 Score: 68.2 bits (165), Expect = 1.0e-11
Identity = 29/87 (33.33%), Postives = 51/87 (58.62%), Query Frame = 0


Query: 143 SFMNVSEYRMVPTCDHAIEMLTRAKIEEVE-SDQRCVICLEDIEKEGECALLLLKMPCLH 202
           SF   + + + P     ++ LTR   +++  + +RC ICLE+    G    +L+ +PC H
Sbjct: 240 SFDETTNFCLGPASKLVVKSLTRKIYDKINYTGERCTICLEEFNAGG----ILVALPCGH 299

Query: 203 VFHEECIIRWLKTSHSCPICRFNMPTE 229
            F +EC ++W +T+H CP+CR+ +P E
Sbjct: 300 DFDDECAVKWFETNHFCPLCRYELPRE 322

[Close]

BLAST of Lcy12g006430 vs. TAIR 10
Match: AT1G55530.1 (RING/U-box superfamily protein )

HSP 1 Score: 67.4 bits (163), Expect = 1.7e-11
Identity = 31/81 (38.27%), Postives = 44/81 (54.32%), Query Frame = 0


Query: 148 SEYRMVPTCDHAIEMLTRAKIEEVESDQRCVICLEDIEKEGECALLLLKMPCLHVFHEEC 207
           + Y   P    A+E L   KIEE     +C +CL+D E   E  L    MPC H FH +C
Sbjct: 197 NRYGTPPAKKEAVEALATVKIEET---LQCSVCLDDFEIGTEAKL----MPCTHKFHSDC 256

Query: 208 IIRWLKTSHSCPICRFNMPTE 229
           ++ WL+   SCP+CR+ +P +
Sbjct: 257 LLPWLELHSSCPVCRYQLPAD 270

[Close]

BLAST of Lcy12g006430 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 66.2 bits (160), Expect = 3.8e-11
Identity = 28/84 (33.33%), Postives = 52/84 (61.90%), Query Frame = 0


Query: 148 SEYRMVPTCDHAIEMLTRAKI--EEVESD-QRCVICLEDIEKEGECALLLLKMPCLHVFH 207
           + Y   P    AI+ L   K+  + ++S+  +C +C+++ E   +    + +MPC HVFH
Sbjct: 184 NRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSD----VKQMPCKHVFH 243

Query: 208 EECIIRWLKTSHSCPICRFNMPTE 229
           ++C++ WL+  +SCP+CRF +PT+
Sbjct: 244 QDCLLPWLELHNSCPVCRFELPTD 263

[Close]

BLAST of Lcy12g006430 vs. TAIR 10
Match: AT3G58720.1 (RING/U-box superfamily protein )

HSP 1 Score: 66.2 bits (160), Expect = 3.8e-11
Identity = 29/52 (55.77%), Postives = 36/52 (69.23%), Query Frame = 0


Query: 172 ESDQRCVICLEDIEKEGECALLLLKMPCLHVFHEECIIRWLKTSHSCPICRF 224
           E D+RC +CLED E + E  +L    PC H+FHEECI+ WLKT   CP+CRF
Sbjct: 139 EDDKRCSVCLEDFEPK-ETVML---TPCKHMFHEECIVPWLKTKGQCPVCRF 186

The following BLAST results are available for this feature:

BLAST of Lcy12g006430 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q940T5	9.7e-12	41.56	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]
Q6AVN2	3.7e-11	44.00	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]
Q9CY62	8.2e-11	37.33	E3 ubiquitin-protein ligase RNF181 OS=Mus musculus OX=10090 GN=Rnf181 PE=1 SV=1	[more]
Q3T0W3	1.8e-10	40.00	E3 ubiquitin-protein ligase RNF181 OS=Bos taurus OX=9913 GN=RNF181 PE=2 SV=1	[more]
Q9P0P0	1.8e-10	38.67	E3 ubiquitin-protein ligase RNF181 OS=Homo sapiens OX=9606 GN=RNF181 PE=1 SV=1	[more]

BLAST of Lcy12g006430 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BQW1	3.8e-51	51.22	uncharacterized protein LOC111004784 OS=Momordica charantia OX=3673 GN=LOC111004...	[more]
A0A6J1HDB7	1.7e-35	41.05	probable E3 ubiquitin-protein ligase ZFP1 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A5A7SNF1	1.4e-32	38.53	RING finger protein 141-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S4E0Q9	1.4e-32	38.53	RING finger protein 141-like OS=Cucumis melo OX=3656 GN=LOC103495859 PE=4 SV=1	[more]
A0A5D3C4K6	1.1e-31	37.35	Putative zinc finger protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]

BLAST of Lcy12g006430 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022131659.1	7.8e-51	51.22	uncharacterized protein LOC111004784 [Momordica charantia]	[more]
XP_022961194.1	3.5e-35	41.05	probable E3 ubiquitin-protein ligase ZFP1 [Cucurbita moschata]	[more]
XP_016901804.1	2.8e-32	38.53	PREDICTED: RING finger protein 141-like [Cucumis melo] >KAA0025781.1 RING finger...	[more]
XP_023511497.1	1.1e-31	38.33	ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucurbita pepo subsp. pep...	[more]
XP_023516070.1	1.8e-31	38.94	E3 ubiquitin-protein ligase RNF165-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lcy12g006430 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59550.1	6.9e-13	41.56	zinc finger (C3HC4-type RING finger) family protein	[more]
AT3G13228.1	1.0e-11	33.33	RING/U-box superfamily protein	[more]
AT1G55530.1	1.7e-11	38.27	RING/U-box superfamily protein	[more]
AT3G19950.1	3.8e-11	33.33	RING/U-box superfamily protein	[more]
AT3G58720.1	3.8e-11	55.77	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 177..221 e-value: 5.2E-6 score: 36.0
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 176..222 e-value: 8.7E-13 score: 48.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 177..222 score: 12.769585
IPR011016	Zinc finger, RING-CH-type	SMART	SM00744	ringv_2	coord: 176..222 e-value: 0.005 score: 9.8
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 143..227 e-value: 5.6E-18 score: 66.4
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 26..227
None	No IPR available	PANTHER	PTHR15710:SF120	F6A14.12 PROTEIN-RELATED	coord: 26..227
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 170..226

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy12g006430.1	Lcy12g006430.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0008270	zinc ion binding