Lcy12g004370 (gene) Sponge gourd (P93075) v1

Overview
NameLcy12g004370
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionPlant protein of unknown function (DUF247)
LocationChr12: 15802175 .. 15803458 (-)
RNA-Seq ExpressionLcy12g004370
SyntenyLcy12g004370
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCCTGGCGAAACAAGTCGGGACGCTGAACTACGAGAAATTGTTAGTGATGGTCAAGAACGAGTGACCACTGAAGAAACTAATCCAGTTGAAAAATCTTCAGAATGCTGCATATACAGGGTTCCGGATGAACTGCGCAGAGTGAAGCCTGAAGCTTATACTCCCAAAGTCATCTCCATCGGCCCTCTTCATCACAATACACAAGGGCTGAGGGCTAATACTTACAAAGACAGGTATTTTAAAGGCTTCCTTGAACTTACCCAAAAAACCGATTCGGAGGTTTTCGAATTTGTGAAAACTTTGGCGACAAGGGTTCGCAATTGTTATGAAGGACCATTTCAGGTGGAGAATAATGATGATTTTTTGAAACTTCTTGCCGTCGATGGTTGTTTCGTGGTCATGTATCTTATCGGTTTTAAATTCCCCCAGTTTGGGCATACAGATACGTCGTTTTTACAGATATTCGGGAATGAAATATTAGGTGATCTAATACTTGTTGAAAACCAACTTCCTTTCTTCCTTCTTGAAGATCTATACAACTTGTGCGTCTCATCTACAAGTTTACAAGACAGGTTGTTCCAACTTGCAAATCACTATTTCATTAAGGTTAACAAAATGAGTTTTGTTCAGGACATTGATGGGGAAGGTCGTCAAATAAATCATTTTGTTGATCTTTTAAGATTGGACATCATTCATTCGCCTCTTGAAAGAGAACCAGTTGAAGACACCTGGTCGCCTACAGTCACAGAGCTCTCCGACTACGGCATCTCTTTGCAGACGAAATATGACCCCCATATGGACGTATGCTTCAGAGAAGGTGAACGCGTTCTCAGATTGCCAAAAATCATAGTATCTGAGACATTCGAAATCCAAATGAGAAACCTCATAGCTTACGAACAATATTATTGCAGCCGCTTCACGACATCGACGGACGATGTACGCAGCTTCGCCTCCTGCTTGAATTCTTTGATCAACACAACAAAGGATGTGGAATTGCTCATTAATGATGGGATCATACAAAACAATTTGGGCAGTATTGAGGAAGTTACGAGCTTTATCAAAAATCTCAGTAAAAACATACACGTTGGACGAAATCTATACTGGTATGAATGTAAATTGATGAAAAATCACTGCAAGCACCGTCGTTATCGGTGGATGACTTTATTGAAACGCAACCATTTGAACACGCCCTGGGCCATCTTCGCCCTTATTTTCACCCTCTACCTCGCTCTTCTCACCACTGCGCAAACGGTCTTAGCTAGTCTCAGCTACCGAAGGTAA

mRNA sequence

ATGGAGCCTGGCGAAACAAGTCGGGACGCTGAACTACGAGAAATTGTTAGTGATGGTCAAGAACGAGTGACCACTGAAGAAACTAATCCAGTTGAAAAATCTTCAGAATGCTGCATATACAGGGTTCCGGATGAACTGCGCAGAGTGAAGCCTGAAGCTTATACTCCCAAAGTCATCTCCATCGGCCCTCTTCATCACAATACACAAGGGCTGAGGGCTAATACTTACAAAGACAGGTATTTTAAAGGCTTCCTTGAACTTACCCAAAAAACCGATTCGGAGGTTTTCGAATTTGTGAAAACTTTGGCGACAAGGGTTCGCAATTGTTATGAAGGACCATTTCAGGTGGAGAATAATGATGATTTTTTGAAACTTCTTGCCGTCGATGGTTGTTTCGTGGTCATGTATCTTATCGGTTTTAAATTCCCCCAGTTTGGGCATACAGATACGTCGTTTTTACAGATATTCGGGAATGAAATATTAGGTGATCTAATACTTGTTGAAAACCAACTTCCTTTCTTCCTTCTTGAAGATCTATACAACTTGTGCGTCTCATCTACAAGTTTACAAGACAGGTTGTTCCAACTTGCAAATCACTATTTCATTAAGGTTAACAAAATGAGTTTTGTTCAGGACATTGATGGGGAAGGTCGTCAAATAAATCATTTTGTTGATCTTTTAAGATTGGACATCATTCATTCGCCTCTTGAAAGAGAACCAGTTGAAGACACCTGGTCGCCTACAGTCACAGAGCTCTCCGACTACGGCATCTCTTTGCAGACGAAATATGACCCCCATATGGACGTATGCTTCAGAGAAGGTGAACGCGTTCTCAGATTGCCAAAAATCATAGTATCTGAGACATTCGAAATCCAAATGAGAAACCTCATAGCTTACGAACAATATTATTGCAGCCGCTTCACGACATCGACGGACGATGTACGCAGCTTCGCCTCCTGCTTGAATTCTTTGATCAACACAACAAAGGATGTGGAATTGCTCATTAATGATGGGATCATACAAAACAATTTGGGCAGTATTGAGGAAGTTACGAGCTTTATCAAAAATCTCAGTAAAAACATACACGTTGGACGAAATCTATACTGGTATGAATGTAAATTGATGAAAAATCACTGCAAGCACCGTCGTTATCGGTGGATGACTTTATTGAAACGCAACCATTTGAACACGCCCTGGGCCATCTTCGCCCTTATTTTCACCCTCTACCTCGCTCTTCTCACCACTGCGCAAACGGTCTTAGCTAGTCTCAGCTACCGAAGGTAA

Coding sequence (CDS)

ATGGAGCCTGGCGAAACAAGTCGGGACGCTGAACTACGAGAAATTGTTAGTGATGGTCAAGAACGAGTGACCACTGAAGAAACTAATCCAGTTGAAAAATCTTCAGAATGCTGCATATACAGGGTTCCGGATGAACTGCGCAGAGTGAAGCCTGAAGCTTATACTCCCAAAGTCATCTCCATCGGCCCTCTTCATCACAATACACAAGGGCTGAGGGCTAATACTTACAAAGACAGGTATTTTAAAGGCTTCCTTGAACTTACCCAAAAAACCGATTCGGAGGTTTTCGAATTTGTGAAAACTTTGGCGACAAGGGTTCGCAATTGTTATGAAGGACCATTTCAGGTGGAGAATAATGATGATTTTTTGAAACTTCTTGCCGTCGATGGTTGTTTCGTGGTCATGTATCTTATCGGTTTTAAATTCCCCCAGTTTGGGCATACAGATACGTCGTTTTTACAGATATTCGGGAATGAAATATTAGGTGATCTAATACTTGTTGAAAACCAACTTCCTTTCTTCCTTCTTGAAGATCTATACAACTTGTGCGTCTCATCTACAAGTTTACAAGACAGGTTGTTCCAACTTGCAAATCACTATTTCATTAAGGTTAACAAAATGAGTTTTGTTCAGGACATTGATGGGGAAGGTCGTCAAATAAATCATTTTGTTGATCTTTTAAGATTGGACATCATTCATTCGCCTCTTGAAAGAGAACCAGTTGAAGACACCTGGTCGCCTACAGTCACAGAGCTCTCCGACTACGGCATCTCTTTGCAGACGAAATATGACCCCCATATGGACGTATGCTTCAGAGAAGGTGAACGCGTTCTCAGATTGCCAAAAATCATAGTATCTGAGACATTCGAAATCCAAATGAGAAACCTCATAGCTTACGAACAATATTATTGCAGCCGCTTCACGACATCGACGGACGATGTACGCAGCTTCGCCTCCTGCTTGAATTCTTTGATCAACACAACAAAGGATGTGGAATTGCTCATTAATGATGGGATCATACAAAACAATTTGGGCAGTATTGAGGAAGTTACGAGCTTTATCAAAAATCTCAGTAAAAACATACACGTTGGACGAAATCTATACTGGTATGAATGTAAATTGATGAAAAATCACTGCAAGCACCGTCGTTATCGGTGGATGACTTTATTGAAACGCAACCATTTGAACACGCCCTGGGCCATCTTCGCCCTTATTTTCACCCTCTACCTCGCTCTTCTCACCACTGCGCAAACGGTCTTAGCTAGTCTCAGCTACCGAAGGTAA

Protein sequence

MEPGETSRDAELREIVSDGQERVTTEETNPVEKSSECCIYRVPDELRRVKPEAYTPKVISIGPLHHNTQGLRANTYKDRYFKGFLELTQKTDSEVFEFVKTLATRVRNCYEGPFQVENNDDFLKLLAVDGCFVVMYLIGFKFPQFGHTDTSFLQIFGNEILGDLILVENQLPFFLLEDLYNLCVSSTSLQDRLFQLANHYFIKVNKMSFVQDIDGEGRQINHFVDLLRLDIIHSPLEREPVEDTWSPTVTELSDYGISLQTKYDPHMDVCFREGERVLRLPKIIVSETFEIQMRNLIAYEQYYCSRFTTSTDDVRSFASCLNSLINTTKDVELLINDGIIQNNLGSIEEVTSFIKNLSKNIHVGRNLYWYECKLMKNHCKHRRYRWMTLLKRNHLNTPWAIFALIFTLYLALLTTAQTVLASLSYRR
Homology
BLAST of Lcy12g004370 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 6.7e-30
Identity = 118/440 (26.82%), Postives = 203/440 (46.14%), Query Frame = 0

Query: 37  CCIYRVPDELRRVKPEAYTPKVISIGPLHHNTQGLR-ANTYKDRYFKGFLELTQKTDSEV 96
           CCI+RVP+    + P+AY PKV+SIGP H+  + L+    +K R  + FL+  +K D E 
Sbjct: 46  CCIFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDEAKKKDVEE 105

Query: 97  FEFVKT---LATRVRNCYEGPFQVENNDDFLKLLAVDGCFVVMYLIGFKFPQFGHTDTSF 156
              VK    L  ++R  Y    +++   D + ++ +DGCF++M  +       G+ + S 
Sbjct: 106 NVLVKAVVDLEDKIRKSYSE--ELKTGHDLMFMMVLDGCFILMVFLIMS----GNIELSE 165

Query: 157 LQIFG-----NEILGDLILVENQLPFFLLEDLYNLCVSSTSLQDRLFQLANHYFIKVNKM 216
             IF      + I  DL+L+ENQ+PFF+L+ LY    S   +   L ++A H+       
Sbjct: 166 DPIFSIPWLLSSIQSDLLLLENQVPFFVLQTLY--VGSKIGVSSDLNRIAFHF------- 225

Query: 217 SFVQDIDGEGR--------QINHFVDLLR---------LDIIHSPLEREPVEDTWSPTVT 276
            F   ID EG         +  H +DL+R          D   SP  +  + +  S  V 
Sbjct: 226 -FKNPIDKEGSYWEKHRNYKAKHLLDLIRETFLPNTSESDKASSPHVQVQLHEGKSGNVP 285

Query: 277 ELSDYGISL-----------------QTKYDPHMDVCFREGERVLRLPKIIVSETFEIQM 336
            +    + L                 ++K D  ++V  ++ +  L++P++          
Sbjct: 286 SVDSKAVPLILSAKRLRLQGIKFRLRRSKEDSILNVRLKKNK--LQIPQLRFDGFISSFF 345

Query: 337 RNLIAYEQYYCSRFTTSTDDVRSFASCLNSLINTTKDVELLINDG-IIQNNLGSIEEVTS 396
            N +A+EQ+Y    T S++++ ++   +  L+N  +DV  L ND  II+N+ GS  EV+ 
Sbjct: 346 LNCVAFEQFY----TDSSNEITTYIVFMGCLLNNEEDVTFLRNDKLIIENHFGSNNEVSE 405

Query: 397 FIKNLSKNIHVGRNLYWYECKLMKNHCK---HRRYRWMTLL----KRNHLNTPWAIFALI 426
           F K +SK++     ++  +   + N  K       +W   L    +  H  +PW   +  
Sbjct: 406 FFKTISKDV-----VFEVDTSYLNNVFKGVNEYTKKWYNGLWAGFRHTHFESPWTFLSSC 458

BLAST of Lcy12g004370 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 2.2e-12
Identity = 58/216 (26.85%), Postives = 97/216 (44.91%), Query Frame = 0

Query: 39  IYRVPDELRRVKPEAYTPKVISIGPLHHNTQGLRANTYKDRYFKGFLELTQKTDSEVFEF 98
           I+ VP  L    P++YTP  +SIGP H     L+   ++   +K  +    +     F F
Sbjct: 45  IFNVPKALMCSHPDSYTPHRVSIGPYH----CLKPELHEMERYKLMIARKIRNQYNSFRF 104

Query: 99  ------VKTLATRVRNCYEGPFQVENNDDFLKLLAVDGCFVVMYLIGFKFPQFGHTDTSF 158
                 ++++  ++R CY   +   N +  L ++AVD  F++ +L   K   F   +T  
Sbjct: 105 HDLVEKLQSMEIKIRACYH-KYIGFNGETLLWIMAVDSSFLIEFL---KIYSFRKVETLI 164

Query: 159 LQIFGNEILGDLILVENQLPFFLLEDLYNLCVSSTSLQDRLF-----QLANHYFIKVNKM 218
            ++  NEIL D++++ENQ+P F+L       + ST   D L       L       V K 
Sbjct: 165 NRVGHNEILRDIMMIENQIPLFVLRKTLEFQLESTESADDLLLSVLTGLCKDLSPLVIKF 224

Query: 219 SFVQDIDGEGRQINHFVDLLRLDIIHSPLEREPVED 244
              Q +  + ++ NH +D L   I+    E E  ED
Sbjct: 225 DDDQILKAQFQECNHILDFLYQMIVPRIEEEELEED 252

BLAST of Lcy12g004370 vs. ExPASy TrEMBL
Match: A0A6J1HBC3 (UPF0481 protein At3g47200-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111462529 PE=4 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 2.6e-77
Identity = 173/396 (43.69%), Postives = 242/396 (61.11%), Query Frame = 0

Query: 34  SSECCIYRVPDELRRVKPEAYTPKVISIGPLHHNTQGLRANTYKDRYFKGFLELTQKTDS 93
           SS+  IY+VP+ LR +KPEAYTP VISIGPLH   + L AN+ K  Y + FL LT+   +
Sbjct: 71  SSDGTIYKVPEPLRSIKPEAYTPTVISIGPLHSGRKDLTANSLKPMYLQNFLNLTKLPTN 130

Query: 94  EVFEFVKTLATRVRNCYEGPFQVENNDDFLKLLAVDGCFVVMYLIGFKFPQFGHTDTSFL 153
            + E VKT   R R CY    ++ N D+F++LL  DGCFVVM+LIG+ F +   +D S L
Sbjct: 131 TIVETVKTWEKRARYCYAESIEM-NRDEFVELLVFDGCFVVMHLIGYSFFELRASDMSNL 190

Query: 154 QIFGNEILGDLILVENQLPFFLLEDLYNLCVSSTSLQD--RLFQLANHYFIKVNK---MS 213
             F  E+  DLIL+ENQLPFFLL+ LY+LC SS  L       +L + YFI+ +K    S
Sbjct: 191 WKFWYELFCDLILLENQLPFFLLQSLYDLCASSQPLLKGVHFIELVHQYFIESHKGGLFS 250

Query: 214 FVQDI--DGEGRQINHFVDLLRLDIIHSPLEREPVEDT-WSPTVTELSDYGISLQTKYDP 273
             + +   G G Q+NHFVDLLRL   H+  +    + T W P  T+L + G+     +  
Sbjct: 251 LKEHVLLAGIGVQVNHFVDLLRLHFTHTRSDETSFQHTFWPPNATKLHECGVI----FKM 310

Query: 274 HMDVCFREGERVLRLPKIIVSETFEIQMRNLIAYEQYYCSRFTTSTDDVRSFASCLNSLI 333
              + F++    L+LP+I + + FE ++RNLIAYEQ  C   +   ++V +FA  +  L+
Sbjct: 311 GKGIAFKDQGGCLQLPQINIYDDFEKRVRNLIAYEQ--CHIGSELRNEVSNFAVFMQCLV 370

Query: 334 NTTKDVELLINDGIIQNNLGSIEEVTSFIKNLSKNIHVGRNLYWYECKLMKNHCKHRRYR 393
            T +DV+LLI  GII NN GSI EVT    NL K+I  G N Y ++CK MK++CK  R+R
Sbjct: 371 QTDQDVKLLIEGGIIHNNFGSINEVTQLFNNLGKHICPGINSYNFDCKRMKDYCKRPRHR 430

Query: 394 WMTLLKRNHLNTPWAIFALIFTLYLALLTTAQTVLA 422
           W++LL+RN+ +TPW   + I  + L  LT  QT++A
Sbjct: 431 WISLLRRNYFSTPWLCASSIAAILLLALTLIQTIVA 459

BLAST of Lcy12g004370 vs. ExPASy TrEMBL
Match: A0A6J1HD53 (UPF0481 protein At3g47200-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462529 PE=4 SV=1)

HSP 1 Score: 299.3 bits (765), Expect = 2.6e-77
Identity = 173/396 (43.69%), Postives = 242/396 (61.11%), Query Frame = 0

Query: 34  SSECCIYRVPDELRRVKPEAYTPKVISIGPLHHNTQGLRANTYKDRYFKGFLELTQKTDS 93
           SS+  IY+VP+ LR +KPEAYTP VISIGPLH   + L AN+ K  Y + FL LT+   +
Sbjct: 170 SSDGTIYKVPEPLRSIKPEAYTPTVISIGPLHSGRKDLTANSLKPMYLQNFLNLTKLPTN 229

Query: 94  EVFEFVKTLATRVRNCYEGPFQVENNDDFLKLLAVDGCFVVMYLIGFKFPQFGHTDTSFL 153
            + E VKT   R R CY    ++ N D+F++LL  DGCFVVM+LIG+ F +   +D S L
Sbjct: 230 TIVETVKTWEKRARYCYAESIEM-NRDEFVELLVFDGCFVVMHLIGYSFFELRASDMSNL 289

Query: 154 QIFGNEILGDLILVENQLPFFLLEDLYNLCVSSTSLQD--RLFQLANHYFIKVNK---MS 213
             F  E+  DLIL+ENQLPFFLL+ LY+LC SS  L       +L + YFI+ +K    S
Sbjct: 290 WKFWYELFCDLILLENQLPFFLLQSLYDLCASSQPLLKGVHFIELVHQYFIESHKGGLFS 349

Query: 214 FVQDI--DGEGRQINHFVDLLRLDIIHSPLEREPVEDT-WSPTVTELSDYGISLQTKYDP 273
             + +   G G Q+NHFVDLLRL   H+  +    + T W P  T+L + G+     +  
Sbjct: 350 LKEHVLLAGIGVQVNHFVDLLRLHFTHTRSDETSFQHTFWPPNATKLHECGVI----FKM 409

Query: 274 HMDVCFREGERVLRLPKIIVSETFEIQMRNLIAYEQYYCSRFTTSTDDVRSFASCLNSLI 333
              + F++    L+LP+I + + FE ++RNLIAYEQ  C   +   ++V +FA  +  L+
Sbjct: 410 GKGIAFKDQGGCLQLPQINIYDDFEKRVRNLIAYEQ--CHIGSELRNEVSNFAVFMQCLV 469

Query: 334 NTTKDVELLINDGIIQNNLGSIEEVTSFIKNLSKNIHVGRNLYWYECKLMKNHCKHRRYR 393
            T +DV+LLI  GII NN GSI EVT    NL K+I  G N Y ++CK MK++CK  R+R
Sbjct: 470 QTDQDVKLLIEGGIIHNNFGSINEVTQLFNNLGKHICPGINSYNFDCKRMKDYCKRPRHR 529

Query: 394 WMTLLKRNHLNTPWAIFALIFTLYLALLTTAQTVLA 422
           W++LL+RN+ +TPW   + I  + L  LT  QT++A
Sbjct: 530 WISLLRRNYFSTPWLCASSIAAILLLALTLIQTIVA 558

BLAST of Lcy12g004370 vs. ExPASy TrEMBL
Match: A0A1S3BD00 (UPF0481 protein At3g47200-like OS=Cucumis melo OX=3656 GN=LOC103488308 PE=4 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 4.4e-77
Identity = 190/433 (43.88%), Postives = 253/433 (58.43%), Query Frame = 0

Query: 7   SRDAELREI----VSDGQERVTTEETNPVEKSSECCIYRVPDELRRVKPEAYTPKVISIG 66
           SRD E++EI    V      ++ E +     S E  IY VP  LR   P+AY+P+VISIG
Sbjct: 16  SRDEEIKEIYDRMVESVNHSISREISISTSFSRERSIYMVPKLLRNGNPKAYSPQVISIG 75

Query: 67  PLH-HNTQG-LRANTYKDRYFKGFLELTQKTDSEVFEFVKTLATRVRNCYEGPFQVENND 126
           PLH + TQ  L     K  Y   FL + +   +E+         R RN Y    ++E  D
Sbjct: 76  PLHYYRTQNDLTIKEKKGSYVLNFLTVAKLGWNEMINKFLCWEERARNYYVETIKME-RD 135

Query: 127 DFLKLLAVDGCFVVMYLIGFKFPQFGHTDTSFLQIFGNEILGDLILVENQLPFFLLEDLY 186
           +F++LL  D CFVVMY+IG    +F   DTSFL  F N I  DL+L+ENQLPFFLL  LY
Sbjct: 136 EFIQLLIYDSCFVVMYIIGSMVAEFRDLDTSFLWRFSNGIFKDLLLLENQLPFFLLHHLY 195

Query: 187 NLCV-SSTSLQDRLF-QLANHYFIKVNK-MSFVQD--IDGEGRQINHFVDLLRLDII--- 246
           NLC  +  SL+D  F +L   YF +V + MS+V +  +D +  ++NH VD LR+ +    
Sbjct: 196 NLCAFAQPSLKDISFIELLRGYFTEVREGMSYVNEGYLDIDANEVNHLVDFLRIHLTQPR 255

Query: 247 HSP----LEREPVEDTWSPTVTELSDYGISLQTKYDPHMDVCFREGERVLRLPKIIVSET 306
           H P       +    +W  T TEL D GIS   +    M+V FRE   VL++PKII+ ++
Sbjct: 256 HIPHFLDFSVDDFLSSWPLTATELHDCGISFHGQKRCMMNVNFRERNGVLKMPKIIIDDS 315

Query: 307 FEIQMRNLIAYEQYYCSRFTTSTDDVRSFASCLNSLINTTKDVELLINDGIIQNNLGSIE 366
           FEI  RN+IAYE  YC      + DV +F   ++ LINT KDV LL++DGIIQN+LGS  
Sbjct: 316 FEILFRNMIAYE--YCH---LKSKDVSNFGMFMHFLINTNKDVSLLVDDGIIQNHLGSTR 375

Query: 367 EVTSFIKNLSKNIHVGRNLYWYECKLMKNHCKHRRYRWMTLLKRNHLNTPWAIFALIFTL 422
           E+     +L KNI V RNLY  EC+ MK +CKHRR+RWMT LKR++  TPWA  + +  +
Sbjct: 376 EIVVLFNDLCKNIMVERNLYSIECRKMKEYCKHRRHRWMTSLKRDYFGTPWAFISFVAAV 435

BLAST of Lcy12g004370 vs. ExPASy TrEMBL
Match: A0A5A7VCL1 (UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002480 PE=4 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 5.8e-77
Identity = 190/433 (43.88%), Postives = 253/433 (58.43%), Query Frame = 0

Query: 7   SRDAELREI----VSDGQERVTTEETNPVEKSSECCIYRVPDELRRVKPEAYTPKVISIG 66
           SRD E++EI    V      ++ E +     S E  IY VP  LR   P+AY+P+VISIG
Sbjct: 16  SRDEEIKEIYDRMVESVNHSISREISISTSFSRERSIYMVPKLLRNGNPKAYSPQVISIG 75

Query: 67  PLH-HNTQG-LRANTYKDRYFKGFLELTQKTDSEVFEFVKTLATRVRNCYEGPFQVENND 126
           PLH + TQ  L     K  Y   FL + +   +E+         R RN Y    ++E  D
Sbjct: 76  PLHYYRTQNDLTIKEKKGSYVLNFLTVAKLGWNEMINKFLCWEERARNYYVETIKME-RD 135

Query: 127 DFLKLLAVDGCFVVMYLIGFKFPQFGHTDTSFLQIFGNEILGDLILVENQLPFFLLEDLY 186
           +F++LL  D CFVVMY+IG    +F   DTSFL  F N I  DL+L+ENQLPFFLL  LY
Sbjct: 136 EFIQLLIYDSCFVVMYIIGSMVAEFRDLDTSFLWRFSNGIFKDLLLLENQLPFFLLHHLY 195

Query: 187 NLCV-SSTSLQDRLF-QLANHYFIKVNK-MSFVQD--IDGEGRQINHFVDLLRLDII--- 246
           NLC  +  SL+D  F +L   YF +V + MS+V +  +D +  ++NH VD LR+ +    
Sbjct: 196 NLCAFAQPSLKDISFIELLRGYFTEVREGMSYVNEGYLDIDANEVNHLVDFLRIHLTQPR 255

Query: 247 HSP----LEREPVEDTWSPTVTELSDYGISLQTKYDPHMDVCFREGERVLRLPKIIVSET 306
           H P       +    +W  T TEL D GIS   +    M+V FRE   VL++PKII+ ++
Sbjct: 256 HIPHFLDFSVDDFLSSWPLTATELHDCGISFHGQKRCMMNVNFRERNGVLKMPKIIIDDS 315

Query: 307 FEIQMRNLIAYEQYYCSRFTTSTDDVRSFASCLNSLINTTKDVELLINDGIIQNNLGSIE 366
           FEI  RN+IAYE  YC      + DV +F   ++ LINT KDV LL++DGIIQN+LGS  
Sbjct: 316 FEILFRNMIAYE--YCH---LKSKDVSNFGMFMHFLINTNKDVSLLVDDGIIQNHLGSTR 375

Query: 367 EVTSFIKNLSKNIHVGRNLYWYECKLMKNHCKHRRYRWMTLLKRNHLNTPWAIFALIFTL 422
           E+     +L KNI V RNLY  EC+ MK +CKHRR+RWMT LKR++  TPWA  + +  +
Sbjct: 376 EIVVLFNDLCKNIIVERNLYSIECRKMKEYCKHRRHRWMTSLKRDYFGTPWAFISFVAAV 435

BLAST of Lcy12g004370 vs. ExPASy TrEMBL
Match: A0A6J1HBL7 (UPF0481 protein At3g47200-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462529 PE=4 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 7.8e-74
Identity = 166/389 (42.67%), Postives = 230/389 (59.13%), Query Frame = 0

Query: 34  SSECCIYRVPDELRRVKPEAYTPKVISIGPLHHNTQGLRANTYKDRYFKGFLELTQKTDS 93
           SS+  IY+VP+ LR +KPEAYTP VISIGPLH   + L AN+ K  Y + FL LT+   +
Sbjct: 170 SSDGTIYKVPEPLRSIKPEAYTPTVISIGPLHSGRKDLTANSLKPMYLQNFLNLTKLPTN 229

Query: 94  EVFEFVKTLATRVRNCYEGPFQVENNDDFLKLLAVDGCFVVMYLIGFKFPQFGHTDTSFL 153
            + E VKT   R R CY    ++ N D+F++LL  DGCFVVM+LIG+ F +   +D S L
Sbjct: 230 TIVETVKTWEKRARYCYAESIEM-NRDEFVELLVFDGCFVVMHLIGYSFFELRASDMSNL 289

Query: 154 QIFGNEILGDLILVENQLPFFLLEDLYNLCVSSTSLQDRLFQLANHYFIKVNKMSFVQDI 213
             F  E+  DLIL+ENQLPFFLL+ LY+LC SS  L                        
Sbjct: 290 WKFWYELFCDLILLENQLPFFLLQSLYDLCASSQPLL----------------------- 349

Query: 214 DGEGRQINHFVDLLRLDIIHSPLEREPVEDT-WSPTVTELSDYGISLQTKYDPHMDVCFR 273
             +G Q+NHFVDLLRL   H+  +    + T W P  T+L + G+     +     + F+
Sbjct: 350 --KGVQVNHFVDLLRLHFTHTRSDETSFQHTFWPPNATKLHECGVI----FKMGKGIAFK 409

Query: 274 EGERVLRLPKIIVSETFEIQMRNLIAYEQYYCSRFTTSTDDVRSFASCLNSLINTTKDVE 333
           +    L+LP+I + + FE ++RNLIAYEQ  C   +   ++V +FA  +  L+ T +DV+
Sbjct: 410 DQGGCLQLPQINIYDDFEKRVRNLIAYEQ--CHIGSELRNEVSNFAVFMQCLVQTDQDVK 469

Query: 334 LLINDGIIQNNLGSIEEVTSFIKNLSKNIHVGRNLYWYECKLMKNHCKHRRYRWMTLLKR 393
           LLI  GII NN GSI EVT    NL K+I  G N Y ++CK MK++CK  R+RW++LL+R
Sbjct: 470 LLIEGGIIHNNFGSINEVTQLFNNLGKHICPGINSYNFDCKRMKDYCKRPRHRWISLLRR 526

Query: 394 NHLNTPWAIFALIFTLYLALLTTAQTVLA 422
           N+ +TPW   + I  + L  LT  QT++A
Sbjct: 530 NYFSTPWLCASSIAAILLLALTLIQTIVA 526

BLAST of Lcy12g004370 vs. NCBI nr
Match: XP_023546104.1 (UPF0481 protein At3g47200-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023546105.1 UPF0481 protein At3g47200-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 305.4 bits (781), Expect = 7.5e-79
Identity = 175/397 (44.08%), Postives = 246/397 (61.96%), Query Frame = 0

Query: 34  SSECCIYRVPDELRRVKPEAYTPKVISIGPLHHNTQGLRANTYKDRYFKGFLELTQKTDS 93
           SS+  IY+VP+ LR +KPEAYTP VISIGPLH   + L AN+ K RY + FL LTQ   +
Sbjct: 71  SSDGTIYKVPEPLRSIKPEAYTPTVISIGPLHSGRKDLMANSLKPRYLQNFLNLTQLPTN 130

Query: 94  EVFEFVKTLATRVRNCYEGPFQVENNDDFLKLLAVDGCFVVMYLIGFKFPQFGHTDTSFL 153
            + E VKT   R R CY    ++ + D+F++LL  DGCFVVM+LI + F +   +D S L
Sbjct: 131 TIVEIVKTWEKRARYCYAESIEM-SRDEFVELLVFDGCFVVMHLISYSFFELRASDMSNL 190

Query: 154 QIFGNEILGDLILVENQLPFFLLEDLYNLCVSSTSLQD--RLFQLANHYFIKVNK---MS 213
             F +E+  DLIL+ENQLPFFLL+ LY+LC SS       R  +L + YFI+ +K    S
Sbjct: 191 WKFWDELFCDLILLENQLPFFLLQSLYDLCASSQPFSKGVRFIELVHQYFIESHKGGLFS 250

Query: 214 FVQDI--DGEGRQINHFVDLLRLDIIHSPLEREPVEDT-WSPTVTELSDYGISLQTKYDP 273
             + +   G G Q+NHFVDLLRL   H+  ++   + T W P  T+L + G+  +T    
Sbjct: 251 LKEHVLLAGIGVQVNHFVDLLRLHFTHTRSDKMSFQHTFWPPNATQLHECGVIFKT---- 310

Query: 274 HMDVCFREGERVLRLPKIIVSETFEIQMRNLIAYEQ-YYCSRFTTSTDDVRSFASCLNSL 333
              + F++    L+LP+I + + FE ++RNLIAYEQ ++CS      ++V +FA  +  L
Sbjct: 311 GKGIAFKDQGGCLQLPQINIYDDFEKRVRNLIAYEQCHFCSEL---RNEVSNFAVFMQCL 370

Query: 334 INTTKDVELLINDGIIQNNLGSIEEVTSFIKNLSKNIHVGRNLYWYECKLMKNHCKHRRY 393
           + T +DV+LLI  GII NN GSI EVT    NL K+I  G N Y  +CK MK++CK  R+
Sbjct: 371 VQTDQDVKLLIEGGIIHNNFGSINEVTQLFNNLGKHICPGINSYNSDCKRMKDYCKRPRH 430

Query: 394 RWMTLLKRNHLNTPWAIFALIFTLYLALLTTAQTVLA 422
           RW++LL+RN+ +TPW   + I  + L  LT  QT++A
Sbjct: 431 RWISLLRRNYFSTPWLCASSIAAILLLALTLIQTIVA 459

BLAST of Lcy12g004370 vs. NCBI nr
Match: XP_023546101.1 (UPF0481 protein At3g47200-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023546102.1 UPF0481 protein At3g47200-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023546103.1 UPF0481 protein At3g47200-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 305.4 bits (781), Expect = 7.5e-79
Identity = 175/397 (44.08%), Postives = 246/397 (61.96%), Query Frame = 0

Query: 34  SSECCIYRVPDELRRVKPEAYTPKVISIGPLHHNTQGLRANTYKDRYFKGFLELTQKTDS 93
           SS+  IY+VP+ LR +KPEAYTP VISIGPLH   + L AN+ K RY + FL LTQ   +
Sbjct: 170 SSDGTIYKVPEPLRSIKPEAYTPTVISIGPLHSGRKDLMANSLKPRYLQNFLNLTQLPTN 229

Query: 94  EVFEFVKTLATRVRNCYEGPFQVENNDDFLKLLAVDGCFVVMYLIGFKFPQFGHTDTSFL 153
            + E VKT   R R CY    ++ + D+F++LL  DGCFVVM+LI + F +   +D S L
Sbjct: 230 TIVEIVKTWEKRARYCYAESIEM-SRDEFVELLVFDGCFVVMHLISYSFFELRASDMSNL 289

Query: 154 QIFGNEILGDLILVENQLPFFLLEDLYNLCVSSTSLQD--RLFQLANHYFIKVNK---MS 213
             F +E+  DLIL+ENQLPFFLL+ LY+LC SS       R  +L + YFI+ +K    S
Sbjct: 290 WKFWDELFCDLILLENQLPFFLLQSLYDLCASSQPFSKGVRFIELVHQYFIESHKGGLFS 349

Query: 214 FVQDI--DGEGRQINHFVDLLRLDIIHSPLEREPVEDT-WSPTVTELSDYGISLQTKYDP 273
             + +   G G Q+NHFVDLLRL   H+  ++   + T W P  T+L + G+  +T    
Sbjct: 350 LKEHVLLAGIGVQVNHFVDLLRLHFTHTRSDKMSFQHTFWPPNATQLHECGVIFKT---- 409

Query: 274 HMDVCFREGERVLRLPKIIVSETFEIQMRNLIAYEQ-YYCSRFTTSTDDVRSFASCLNSL 333
              + F++    L+LP+I + + FE ++RNLIAYEQ ++CS      ++V +FA  +  L
Sbjct: 410 GKGIAFKDQGGCLQLPQINIYDDFEKRVRNLIAYEQCHFCSEL---RNEVSNFAVFMQCL 469

Query: 334 INTTKDVELLINDGIIQNNLGSIEEVTSFIKNLSKNIHVGRNLYWYECKLMKNHCKHRRY 393
           + T +DV+LLI  GII NN GSI EVT    NL K+I  G N Y  +CK MK++CK  R+
Sbjct: 470 VQTDQDVKLLIEGGIIHNNFGSINEVTQLFNNLGKHICPGINSYNSDCKRMKDYCKRPRH 529

Query: 394 RWMTLLKRNHLNTPWAIFALIFTLYLALLTTAQTVLA 422
           RW++LL+RN+ +TPW   + I  + L  LT  QT++A
Sbjct: 530 RWISLLRRNYFSTPWLCASSIAAILLLALTLIQTIVA 558

BLAST of Lcy12g004370 vs. NCBI nr
Match: XP_022961897.1 (UPF0481 protein At3g47200-like isoform X3 [Cucurbita moschata] >XP_022961898.1 UPF0481 protein At3g47200-like isoform X3 [Cucurbita moschata] >XP_022961899.1 UPF0481 protein At3g47200-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 299.3 bits (765), Expect = 5.3e-77
Identity = 173/396 (43.69%), Postives = 242/396 (61.11%), Query Frame = 0

Query: 34  SSECCIYRVPDELRRVKPEAYTPKVISIGPLHHNTQGLRANTYKDRYFKGFLELTQKTDS 93
           SS+  IY+VP+ LR +KPEAYTP VISIGPLH   + L AN+ K  Y + FL LT+   +
Sbjct: 71  SSDGTIYKVPEPLRSIKPEAYTPTVISIGPLHSGRKDLTANSLKPMYLQNFLNLTKLPTN 130

Query: 94  EVFEFVKTLATRVRNCYEGPFQVENNDDFLKLLAVDGCFVVMYLIGFKFPQFGHTDTSFL 153
            + E VKT   R R CY    ++ N D+F++LL  DGCFVVM+LIG+ F +   +D S L
Sbjct: 131 TIVETVKTWEKRARYCYAESIEM-NRDEFVELLVFDGCFVVMHLIGYSFFELRASDMSNL 190

Query: 154 QIFGNEILGDLILVENQLPFFLLEDLYNLCVSSTSLQD--RLFQLANHYFIKVNK---MS 213
             F  E+  DLIL+ENQLPFFLL+ LY+LC SS  L       +L + YFI+ +K    S
Sbjct: 191 WKFWYELFCDLILLENQLPFFLLQSLYDLCASSQPLLKGVHFIELVHQYFIESHKGGLFS 250

Query: 214 FVQDI--DGEGRQINHFVDLLRLDIIHSPLEREPVEDT-WSPTVTELSDYGISLQTKYDP 273
             + +   G G Q+NHFVDLLRL   H+  +    + T W P  T+L + G+     +  
Sbjct: 251 LKEHVLLAGIGVQVNHFVDLLRLHFTHTRSDETSFQHTFWPPNATKLHECGVI----FKM 310

Query: 274 HMDVCFREGERVLRLPKIIVSETFEIQMRNLIAYEQYYCSRFTTSTDDVRSFASCLNSLI 333
              + F++    L+LP+I + + FE ++RNLIAYEQ  C   +   ++V +FA  +  L+
Sbjct: 311 GKGIAFKDQGGCLQLPQINIYDDFEKRVRNLIAYEQ--CHIGSELRNEVSNFAVFMQCLV 370

Query: 334 NTTKDVELLINDGIIQNNLGSIEEVTSFIKNLSKNIHVGRNLYWYECKLMKNHCKHRRYR 393
            T +DV+LLI  GII NN GSI EVT    NL K+I  G N Y ++CK MK++CK  R+R
Sbjct: 371 QTDQDVKLLIEGGIIHNNFGSINEVTQLFNNLGKHICPGINSYNFDCKRMKDYCKRPRHR 430

Query: 394 WMTLLKRNHLNTPWAIFALIFTLYLALLTTAQTVLA 422
           W++LL+RN+ +TPW   + I  + L  LT  QT++A
Sbjct: 431 WISLLRRNYFSTPWLCASSIAAILLLALTLIQTIVA 459

BLAST of Lcy12g004370 vs. NCBI nr
Match: XP_022961893.1 (UPF0481 protein At3g47200-like isoform X1 [Cucurbita moschata] >XP_022961894.1 UPF0481 protein At3g47200-like isoform X1 [Cucurbita moschata] >XP_022961895.1 UPF0481 protein At3g47200-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 299.3 bits (765), Expect = 5.3e-77
Identity = 173/396 (43.69%), Postives = 242/396 (61.11%), Query Frame = 0

Query: 34  SSECCIYRVPDELRRVKPEAYTPKVISIGPLHHNTQGLRANTYKDRYFKGFLELTQKTDS 93
           SS+  IY+VP+ LR +KPEAYTP VISIGPLH   + L AN+ K  Y + FL LT+   +
Sbjct: 170 SSDGTIYKVPEPLRSIKPEAYTPTVISIGPLHSGRKDLTANSLKPMYLQNFLNLTKLPTN 229

Query: 94  EVFEFVKTLATRVRNCYEGPFQVENNDDFLKLLAVDGCFVVMYLIGFKFPQFGHTDTSFL 153
            + E VKT   R R CY    ++ N D+F++LL  DGCFVVM+LIG+ F +   +D S L
Sbjct: 230 TIVETVKTWEKRARYCYAESIEM-NRDEFVELLVFDGCFVVMHLIGYSFFELRASDMSNL 289

Query: 154 QIFGNEILGDLILVENQLPFFLLEDLYNLCVSSTSLQD--RLFQLANHYFIKVNK---MS 213
             F  E+  DLIL+ENQLPFFLL+ LY+LC SS  L       +L + YFI+ +K    S
Sbjct: 290 WKFWYELFCDLILLENQLPFFLLQSLYDLCASSQPLLKGVHFIELVHQYFIESHKGGLFS 349

Query: 214 FVQDI--DGEGRQINHFVDLLRLDIIHSPLEREPVEDT-WSPTVTELSDYGISLQTKYDP 273
             + +   G G Q+NHFVDLLRL   H+  +    + T W P  T+L + G+     +  
Sbjct: 350 LKEHVLLAGIGVQVNHFVDLLRLHFTHTRSDETSFQHTFWPPNATKLHECGVI----FKM 409

Query: 274 HMDVCFREGERVLRLPKIIVSETFEIQMRNLIAYEQYYCSRFTTSTDDVRSFASCLNSLI 333
              + F++    L+LP+I + + FE ++RNLIAYEQ  C   +   ++V +FA  +  L+
Sbjct: 410 GKGIAFKDQGGCLQLPQINIYDDFEKRVRNLIAYEQ--CHIGSELRNEVSNFAVFMQCLV 469

Query: 334 NTTKDVELLINDGIIQNNLGSIEEVTSFIKNLSKNIHVGRNLYWYECKLMKNHCKHRRYR 393
            T +DV+LLI  GII NN GSI EVT    NL K+I  G N Y ++CK MK++CK  R+R
Sbjct: 470 QTDQDVKLLIEGGIIHNNFGSINEVTQLFNNLGKHICPGINSYNFDCKRMKDYCKRPRHR 529

Query: 394 WMTLLKRNHLNTPWAIFALIFTLYLALLTTAQTVLA 422
           W++LL+RN+ +TPW   + I  + L  LT  QT++A
Sbjct: 530 WISLLRRNYFSTPWLCASSIAAILLLALTLIQTIVA 558

BLAST of Lcy12g004370 vs. NCBI nr
Match: XP_008445209.1 (PREDICTED: UPF0481 protein At3g47200-like [Cucumis melo])

HSP 1 Score: 298.5 bits (763), Expect = 9.1e-77
Identity = 190/433 (43.88%), Postives = 253/433 (58.43%), Query Frame = 0

Query: 7   SRDAELREI----VSDGQERVTTEETNPVEKSSECCIYRVPDELRRVKPEAYTPKVISIG 66
           SRD E++EI    V      ++ E +     S E  IY VP  LR   P+AY+P+VISIG
Sbjct: 16  SRDEEIKEIYDRMVESVNHSISREISISTSFSRERSIYMVPKLLRNGNPKAYSPQVISIG 75

Query: 67  PLH-HNTQG-LRANTYKDRYFKGFLELTQKTDSEVFEFVKTLATRVRNCYEGPFQVENND 126
           PLH + TQ  L     K  Y   FL + +   +E+         R RN Y    ++E  D
Sbjct: 76  PLHYYRTQNDLTIKEKKGSYVLNFLTVAKLGWNEMINKFLCWEERARNYYVETIKME-RD 135

Query: 127 DFLKLLAVDGCFVVMYLIGFKFPQFGHTDTSFLQIFGNEILGDLILVENQLPFFLLEDLY 186
           +F++LL  D CFVVMY+IG    +F   DTSFL  F N I  DL+L+ENQLPFFLL  LY
Sbjct: 136 EFIQLLIYDSCFVVMYIIGSMVAEFRDLDTSFLWRFSNGIFKDLLLLENQLPFFLLHHLY 195

Query: 187 NLCV-SSTSLQDRLF-QLANHYFIKVNK-MSFVQD--IDGEGRQINHFVDLLRLDII--- 246
           NLC  +  SL+D  F +L   YF +V + MS+V +  +D +  ++NH VD LR+ +    
Sbjct: 196 NLCAFAQPSLKDISFIELLRGYFTEVREGMSYVNEGYLDIDANEVNHLVDFLRIHLTQPR 255

Query: 247 HSP----LEREPVEDTWSPTVTELSDYGISLQTKYDPHMDVCFREGERVLRLPKIIVSET 306
           H P       +    +W  T TEL D GIS   +    M+V FRE   VL++PKII+ ++
Sbjct: 256 HIPHFLDFSVDDFLSSWPLTATELHDCGISFHGQKRCMMNVNFRERNGVLKMPKIIIDDS 315

Query: 307 FEIQMRNLIAYEQYYCSRFTTSTDDVRSFASCLNSLINTTKDVELLINDGIIQNNLGSIE 366
           FEI  RN+IAYE  YC      + DV +F   ++ LINT KDV LL++DGIIQN+LGS  
Sbjct: 316 FEILFRNMIAYE--YCH---LKSKDVSNFGMFMHFLINTNKDVSLLVDDGIIQNHLGSTR 375

Query: 367 EVTSFIKNLSKNIHVGRNLYWYECKLMKNHCKHRRYRWMTLLKRNHLNTPWAIFALIFTL 422
           E+     +L KNI V RNLY  EC+ MK +CKHRR+RWMT LKR++  TPWA  + +  +
Sbjct: 376 EIVVLFNDLCKNIMVERNLYSIECRKMKEYCKHRRHRWMTSLKRDYFGTPWAFISFVAAV 435

BLAST of Lcy12g004370 vs. TAIR 10
Match: AT4G31980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247, plant (InterPro:IPR004158), Protein of unknown function DUF862, eukaryotic (InterPro:IPR008580); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF247) (TAIR:AT5G11290.1); Has 1967 Blast hits to 1844 proteins in 183 species: Archae - 0; Bacteria - 6; Metazoa - 223; Fungi - 83; Plants - 1477; Viruses - 0; Other Eukaryotes - 178 (source: NCBI BLink). )

HSP 1 Score: 190.7 bits (483), Expect = 2.5e-48
Identity = 130/407 (31.94%), Postives = 219/407 (53.81%), Query Frame = 0

Query: 34  SSECCIYRVPDELRRVKPEAYTPKVISIGPLHHNTQGLRA-NTYKDRYFKGFLELTQKTD 93
           S++CCIY+VP++LRR+ P+AYTP+++S GPLH   + L+A    K RY   F+  T  + 
Sbjct: 290 STKCCIYKVPNKLRRLNPDAYTPRLVSFGPLHRGKEELQAMEDQKYRYLLSFIPRTNSSL 349

Query: 94  SEVFEFVKTLATRVRNCYEGPFQVENNDDFLKLLAVDGCFVVMYLIGFKFPQF-GHTDTS 153
            ++    +T     R+CY    ++ ++D+F+++L VDG F+V  L+   +P+  G  D  
Sbjct: 350 EDLVRLARTWEQNARSCYAEDVKL-HSDEFVEMLVVDGSFLVELLLRSHYPRLRGEND-- 409

Query: 154 FLQIFGN-----EILGDLILVENQLPFFLLEDLYNLCVSSTSL-QDRLFQLANHYFIKVN 213
             +IFGN     ++  D+IL+ENQLPFF++++++ L ++        + QLA  +F    
Sbjct: 410 --RIFGNSMMITDVCRDMILIENQLPFFVVKEIFLLLLNYYQQGTPSIIQLAQRHF---- 469

Query: 214 KMSFVQDIDGEG--RQINHFVDLLR---LDIIHSPLEREPVEDTWSPTVTELSDYGISLQ 273
              F+  ID E    +  HFVDLLR   L      LE   V+   +P  TEL   G+  +
Sbjct: 470 -SYFLSRIDDEKFITEPEHFVDLLRSCYLPQFPIKLEYTTVKVDNAPEATELHTAGVRFK 529

Query: 274 TKYDPH--MDVCFREGERVLRLPKIIVSETFEIQMRNLIAYEQYYCSRFTTSTDDVRSFA 333
                   +D+ F +G  VL++P I+V +  E   +N+I +EQ  C     S  +   + 
Sbjct: 530 PAETSSCLLDISFADG--VLKIPTIVVDDLTESLYKNIIGFEQCRC-----SNKNFLDYI 589

Query: 334 SCLNSLINTTKDVELLINDGIIQNNLGSIEEVTSFIKNLSKNIHVGRNLYW-YECKLMKN 393
             L   I +  D +LLI+ GII N LG+  +V++   ++SK +   R  Y+    + ++ 
Sbjct: 590 MLLGCFIKSPTDADLLIHSGIIVNYLGNSVDVSNLFNSISKEVIYDRRFYFSMLSENLQA 649

Query: 394 HCKHRRYRWMTLLKRNHLNTPWAIFALIFTLYLALLTTAQTVLASLS 425
           +C     RW  +L+R++ + PWA+ ++   L L LLT  Q+V + L+
Sbjct: 650 YCNTPWNRWKAILRRDYFHNPWAVASVFAALLLLLLTFIQSVCSILA 679

BLAST of Lcy12g004370 vs. TAIR 10
Match: AT3G50150.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 144.4 bits (363), Expect = 2.1e-34
Identity = 126/457 (27.57%), Postives = 223/457 (48.80%), Query Frame = 0

Query: 2   EPGETSRDAELREIVSDGQERVTTEETNPVEKSSECCIYRVPDELRRVKPEAYTPKVISI 61
           +P ET R+  +  I    ++ ++ + TN  +K    CIYRVP  L+    ++Y P+ +SI
Sbjct: 59  KPRET-REEWVISIKDKMEKALSYDATNSWDK---LCIYRVPFYLQENDKKSYLPQTVSI 118

Query: 62  GPLHHNTQGLR-ANTYKDRYFKGFLELTQKTDSEVFEFVKTLATRVRNCYEGPFQVENND 121
           GP HH    LR    +K R     +  T+       + +K L    R CY+GP  ++N++
Sbjct: 119 GPYHHGKVHLRPMERHKWRAVNMIMARTKHNIEMYIDAMKELEEEARACYQGPIDMKNSN 178

Query: 122 DFLKLLAVDGCFVVMY----LIGFKFPQFGHTDTSFLQI-FGNEILGDLILVENQLPFFL 181
           +F ++L +DGCFV+      + GF+   +   D  F +    + I  D+I++ENQLP F+
Sbjct: 179 EFTEMLVLDGCFVLELFKGTIQGFQKIGYARNDPVFAKRGLMHSIQRDMIMLENQLPLFV 238

Query: 182 LEDLYNLCVSSTSLQDRLFQLANHYFIKVNKMSFV-----QDIDGEGR--QINHFVDLLR 241
           L+ L  L   + +    + ++A  +F  +   S V     + +D + +  ++     L  
Sbjct: 239 LDRLLGLQTGTPNQTGIVAEVAVRFFKTLMPTSEVLTKSERSLDSQEKSDELGDNGGLHC 298

Query: 242 LDIIHSPL--------EREPVED--------TWSPTVTELSDYGISLQTKYDPHM-DVCF 301
           LD+ H  L        +  P ED             VTEL   G++   K    + D+ F
Sbjct: 299 LDVFHRSLIQSSETTNQGTPYEDMSMVEKQQQLIHCVTELRGAGVNFMRKETGQLWDIEF 358

Query: 302 REGERVLRLPKIIVSETFEIQMRNLIAYEQYYCSRFTTSTDDVRSFASCLNSLINTTKDV 361
           + G   L++PK+++ +  +    NLIA+EQ +    T S++++ S+   +++LIN+++DV
Sbjct: 359 KNG--YLKIPKLLIHDGTKSLFSNLIAFEQCH----TQSSNNITSYIIFMDNLINSSQDV 418

Query: 362 ELLINDGIIQNNLGSIEEVTSFIKNLSKNIHVGRNLYWYECKLMKNHCKHRRYRWMTL-- 421
             L +DGII++ LGS  EV      L K + +      Y  +L +   ++   +W +L  
Sbjct: 419 SYLHHDGIIEHWLGSDSEVADLFNRLCKEV-IFDPKDGYLSQLSREVNRYYSRKWNSLKA 478

Query: 422 -LKRNHLNTPWAIFALIFTLYLALLTTAQTVLASLSY 426
            L++ + N PWA F+    + L  LT  Q+  A  +Y
Sbjct: 479 TLRQKYFNNPWAYFSFSAAVILLFLTFFQSFFAVYAY 504

BLAST of Lcy12g004370 vs. TAIR 10
Match: AT3G50160.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 137.9 bits (346), Expect = 1.9e-32
Identity = 122/444 (27.48%), Postives = 212/444 (47.75%), Query Frame = 0

Query: 5   ETSRDAELREI-VSDGQERVTTEETNPVEKSSECCIYRVPDELRRVKPEAYTPKVISIGP 64
           E   + +LREI V    +++ T   N        CIYRVP  L+    ++Y P+++SIGP
Sbjct: 71  EDKNEQKLREIWVISLNDKMKTLGDNATTSWDNLCIYRVPPYLQENDTKSYMPQIVSIGP 130

Query: 65  LHHNTQGLRANTYKDRYFKGFLELTQKTDSEVF-EFVKTLATRVRNCYEGPFQVENNDDF 124
            HH  + L             +    K D E++ + +K L  + R CY+GP  + N ++F
Sbjct: 131 YHHGHKHLMPMERHKWRAVNMVMARAKHDIEMYIDAMKELEEKARACYQGPINM-NRNEF 190

Query: 125 LKLLAVDGCFVVMYLIGFK--FPQFGHTDTSFLQIFG-----NEILGDLILVENQLPFFL 184
           +++L +DG F++    G    F + G+       +FG       I  D++++ENQLP+ +
Sbjct: 191 IEMLVLDGVFIIEIFKGTSEGFQEIGYAPND--PVFGMRGLMQSIRRDMVMLENQLPWSV 250

Query: 185 LEDLYNLCVSSTSLQDRL-FQLANHYFIKVNKMSFVQDIDGEGRQINHFVDLLRLDIIHS 244
           L+ L  L      + D++  QL   +F  +     V   +G      H +D+LR  ++ S
Sbjct: 251 LKGLLQL--QRPDVLDKVNVQLFQPFFQPLLPTREVLTEEGG----LHCLDVLRRGLLQS 310

Query: 245 P---------LEREPVEDTWSPTVTELSDYGISLQTKYDPHM-DVCFREGERVLRLPKII 304
                     + ++P +      VTEL + G+    K   H  D+ F+ G   L++PK++
Sbjct: 311 SGTSDEDMSMVNKQPQQ--LIHCVTELRNAGVEFMRKETGHFWDIEFKNG--YLKIPKLL 370

Query: 305 VSETFEIQMRNLIAYEQYYCSRFTTSTDDVRSFASCLNSLINTTKDVELLINDGIIQNNL 364
           + +  +    NLIA+EQ +      S+  + S+   +++LIN+++DV  L + GII+N L
Sbjct: 371 IHDGTKSLFLNLIAFEQCHIK----SSKKITSYIIFMDNLINSSEDVSYLHHYGIIENWL 430

Query: 365 GSIEEVTSFIKNLSKNIHVGRNLYWYECKLMKNHCKHRRYRWMTL---LKRNHLNTPWAI 424
           GS  EV+     L K +    N  +      + +  +RR +W  L   L+  + N PWA 
Sbjct: 431 GSDSEVSDLFNGLGKEVIFDPNDGYLSALTGEVNIYYRR-KWNYLKATLRHKYFNNPWAY 490

Query: 425 FALIFTLYLALLTTAQTVLASLSY 426
           F+ I  + L + T  Q+  A  +Y
Sbjct: 491 FSFIAAVTLLIFTFCQSFFAVFAY 496

BLAST of Lcy12g004370 vs. TAIR 10
Match: AT2G28580.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 134.4 bits (337), Expect = 2.1e-31
Identity = 127/426 (29.81%), Postives = 193/426 (45.31%), Query Frame = 0

Query: 37  CCIYRVPDELRRVKPEAYTPKVISIGPLHHNTQGLRANTYKD--RYFKGF-LELTQKT-- 96
           CCIYRVPD LR+V PEAYTP+++ IGPLHH+ +      YK   RY     +EL +K   
Sbjct: 109 CCIYRVPDRLRKVNPEAYTPQMLLIGPLHHSKKAEALKRYKTDLRYLNYLNMELHKKKCL 168

Query: 97  --------DSEVFEFVKTLATR---VRNCYEGPFQVENNDDFLKLLAVDGCFVVMYLIGF 156
                   D  V EF + +      +R+ Y       N  DF++++  D  F++++ I  
Sbjct: 169 DSIADIYGDQPVKEFRRIIEINEKFIRDSYAESTIWINTKDFVEMILHDSVFILLFFI-- 228

Query: 157 KFPQFGHT-------DTSFLQ---IFGNEILGDLILVENQLPFFLLEDLYNLCVSSTSLQ 216
              Q G T       D  F Q   I    IL DLIL+ENQLP+ LLE L+    S+ + +
Sbjct: 229 ---QTGSTLNFNKKEDILFNQSRLINATAILEDLILLENQLPYALLEKLFEPFSSNVNTK 288

Query: 217 DRLFQLANHYFIKVNKMSFVQDIDGEGRQINHFVDLLRL----------DIIHSPLEREP 276
           +    +    F    K+        E  +  HF DL R           + I+      P
Sbjct: 289 ETFRDITLRAFRFEGKIK-------EEVRFQHFTDLFRCVRVSTLSLTEEQINIAKNEPP 348

Query: 277 VEDTWSPTVTELSDYG---ISLQTKYDPHMDVCFREGERVLRLPKIIVSETFEIQMRNLI 336
                     +L   G   +++  + D  + + F++G  +L++P   V +  E  +RNL+
Sbjct: 349 KSRKIMYNADKLDSAGVNFVNVDEENDLSLVITFKDG--ILKMPCFTVEDNTERVVRNLM 408

Query: 337 AYEQYYCSRFTTSTDDVRSFASCLNSLINTTKDVELLINDGIIQNNLGSIEEVTSFIKNL 396
           A EQ +  R T   D    + S L+ LINT +DV+LL   GI++N LG    VT  +  L
Sbjct: 409 ALEQCHYPRTTFVCD----YISFLDFLINTDQDVDLLAKKGIVKNWLGHQGSVTEMVNKL 468

Query: 397 SKNIHVGRNLYWYECKLMKNHCKHRRYRWMTLLKRNHLNTPWAIFALIFTLYLALLTTAQ 424
              +    + Y    + +  H  +R  R +  L+R +    W   A I  + L +LT  Q
Sbjct: 469 CLGLVDFGSHYSDIVENLNKHYDNRLNRSVGTLRRVYFKDLWTGTATIAAVVLLVLTLIQ 516

BLAST of Lcy12g004370 vs. TAIR 10
Match: AT3G47200.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 133.3 bits (334), Expect = 4.7e-31
Identity = 118/440 (26.82%), Postives = 203/440 (46.14%), Query Frame = 0

Query: 37  CCIYRVPDELRRVKPEAYTPKVISIGPLHHNTQGLR-ANTYKDRYFKGFLELTQKTDSEV 96
           CCI+RVP+    + P+AY PKV+SIGP H+  + L+    +K R  + FL+  +K D E 
Sbjct: 46  CCIFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDEAKKKDVEE 105

Query: 97  FEFVKT---LATRVRNCYEGPFQVENNDDFLKLLAVDGCFVVMYLIGFKFPQFGHTDTSF 156
              VK    L  ++R  Y    +++   D + ++ +DGCF++M  +       G+ + S 
Sbjct: 106 NVLVKAVVDLEDKIRKSYSE--ELKTGHDLMFMMVLDGCFILMVFLIMS----GNIELSE 165

Query: 157 LQIFG-----NEILGDLILVENQLPFFLLEDLYNLCVSSTSLQDRLFQLANHYFIKVNKM 216
             IF      + I  DL+L+ENQ+PFF+L+ LY    S   +   L ++A H+       
Sbjct: 166 DPIFSIPWLLSSIQSDLLLLENQVPFFVLQTLY--VGSKIGVSSDLNRIAFHF------- 225

Query: 217 SFVQDIDGEGR--------QINHFVDLLR---------LDIIHSPLEREPVEDTWSPTVT 276
            F   ID EG         +  H +DL+R          D   SP  +  + +  S  V 
Sbjct: 226 -FKNPIDKEGSYWEKHRNYKAKHLLDLIRETFLPNTSESDKASSPHVQVQLHEGKSGNVP 285

Query: 277 ELSDYGISL-----------------QTKYDPHMDVCFREGERVLRLPKIIVSETFEIQM 336
            +    + L                 ++K D  ++V  ++ +  L++P++          
Sbjct: 286 SVDSKAVPLILSAKRLRLQGIKFRLRRSKEDSILNVRLKKNK--LQIPQLRFDGFISSFF 345

Query: 337 RNLIAYEQYYCSRFTTSTDDVRSFASCLNSLINTTKDVELLINDG-IIQNNLGSIEEVTS 396
            N +A+EQ+Y    T S++++ ++   +  L+N  +DV  L ND  II+N+ GS  EV+ 
Sbjct: 346 LNCVAFEQFY----TDSSNEITTYIVFMGCLLNNEEDVTFLRNDKLIIENHFGSNNEVSE 405

Query: 397 FIKNLSKNIHVGRNLYWYECKLMKNHCK---HRRYRWMTLL----KRNHLNTPWAIFALI 426
           F K +SK++     ++  +   + N  K       +W   L    +  H  +PW   +  
Sbjct: 406 FFKTISKDV-----VFEVDTSYLNNVFKGVNEYTKKWYNGLWAGFRHTHFESPWTFLSSC 458

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SD536.7e-3026.82UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1[more]
P0C8972.2e-1226.85Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 ... [more]
Match NameE-valueIdentityDescription
A0A6J1HBC32.6e-7743.69UPF0481 protein At3g47200-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1HD532.6e-7743.69UPF0481 protein At3g47200-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A1S3BD004.4e-7743.88UPF0481 protein At3g47200-like OS=Cucumis melo OX=3656 GN=LOC103488308 PE=4 SV=1[more]
A0A5A7VCL15.8e-7743.88UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00248... [more]
A0A6J1HBL77.8e-7442.67UPF0481 protein At3g47200-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
XP_023546104.17.5e-7944.08UPF0481 protein At3g47200-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_02354... [more]
XP_023546101.17.5e-7944.08UPF0481 protein At3g47200-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_02354... [more]
XP_022961897.15.3e-7743.69UPF0481 protein At3g47200-like isoform X3 [Cucurbita moschata] >XP_022961898.1 U... [more]
XP_022961893.15.3e-7743.69UPF0481 protein At3g47200-like isoform X1 [Cucurbita moschata] >XP_022961894.1 U... [more]
XP_008445209.19.1e-7743.88PREDICTED: UPF0481 protein At3g47200-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT4G31980.12.5e-4831.94unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247,... [more]
AT3G50150.12.1e-3427.57Plant protein of unknown function (DUF247) [more]
AT3G50160.11.9e-3227.48Plant protein of unknown function (DUF247) [more]
AT2G28580.12.1e-3129.81Plant protein of unknown function (DUF247) [more]
AT3G47200.14.7e-3126.82Plant protein of unknown function (DUF247) [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004158Protein of unknown function DUF247, plantPFAMPF03140DUF247coord: 39..412
e-value: 2.2E-85
score: 287.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..29
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30
NoneNo IPR availablePANTHERPTHR31170BNAC04G53230D PROTEINcoord: 23..421

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy12g004370.1Lcy12g004370.1mRNA