Lcy12g004200 (gene) Sponge gourd (P93075) v1

Overview
NameLcy12g004200
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRibonuclease H-like superfamily protein
LocationChr12: 14692257 .. 14693255 (+)
RNA-Seq ExpressionLcy12g004200
SyntenyLcy12g004200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GGCCTAATAAAGGAATGCAATCGTATAATGGCGAAGTTCTGGTGGGGGGAGGAGGGGGTGAAAAGGAGGATCCATTAGGCCTCGTGGAGGAGAGTGAGTGTGTCAAAGTTTAATGGAGGGTTGGGTTTTAGGGATCTTGAACTGTTTAACAGGGCCCTGTTGGCGAAACAAGGGTGGAGGTTAATAGACAATCCTGATTCCCTTTTGGGGAAAGTTCTTAAGGGTAGGTACTTCTCGAGATGTTCATTTCTAGAGGCCGAAAGGAGAAGCAACGCCTCTTATGTGTGGAGGAGTTTGGTGTGGGGAAGGGGTCTTTTGGAGGAAGGTTTACGGTGGAGGGTGGGAAATGGGAGTTCTGTTAAAATATTGGGGGATAGGTGGATTCCGAGGGCTCCGTCACTGAGGATTCTAATGGGGGAAGGGATTGATCCAGATGCGAGAGTATCAGAGCTTTTGAGTCCTGAAGGGGCATGGAATGTGGATATGGTTCAGGCGATGTTTAAAGGGGATGATGTGCAGGCCATCCTTGGCATTCCTCGTCCTAGAATGGCCTGCCCGGATAGGCTAGTTTGGCATTGTGAGAAGGATGGGATCTACTCGGTGAAGAGTGGGTACAATCTAGCTTGTTCCATTAGAGAGATTCCTAGTTCCTCAAATTGGGAGGAAGTGCAGAAATGGTGGAAGTTCTTCTGGACAAGGGAAATTCCTAGTAAGATAAAATTTTTTGCTTGGAGGCTGTTCCATGACCTCCTGCCAACTGAAACAAATCTAAGGAGGAGGGGGATGGATATTTATCAAGGGTGTCAAAGGTGCAAATTGGAAACGGAGACTACCTTTCATGCCATCTGGGAATGTGTGGAAGTGAAGTGTTGGTGGCGATGCTCGCCGTTTTATCCCCGCTCAGGTCCCCCGAGCATAAGAAGTGTGGCTGACCTGCTGTGGTGGTGCAGGGAGAATTTGTTGAATGAGTGTTTTGAGGAGTTCGTGGTGATGTGCTAG

mRNA sequence

GGCCTAATAAAGGAATGCAATCGTATAATGGCGAAGTTCTGGTGGGGGGAGGAGGGGGTGAAAAGGAGGATCCATTAGGCCTCGTGGAGGAGAGTGAGGCCCTGTTGGCGAAACAAGGGTGGAGGTTAATAGACAATCCTGATTCCCTTTTGGGGAAAGTTCTTAAGGGTAGGTACTTCTCGAGATGTTCATTTCTAGAGGCCGAAAGGAGAAGCAACGCCTCTTATGTGTGGAGGAGTTTGGTGTGGGGAAGGGGTCTTTTGGAGGAAGGTTTACGGTGGAGGGTGGGAAATGGGAGTTCTGTTAAAATATTGGGGGATAGGTGGATTCCGAGGGCTCCGTCACTGAGGATTCTAATGGGGGAAGGGATTGATCCAGATGCGAGAGTATCAGAGCTTTTGAGTCCTGAAGGGGCATGGAATGTGGATATGGTTCAGGCGATGTTTAAAGGGGATGATGTGCAGGCCATCCTTGGCATTCCTCGTCCTAGAATGGCCTGCCCGGATAGGCTAGTTTGGCATTGTGAGAAGGATGGGATCTACTCGGTGAAGAGTGGGTACAATCTAGCTTGTTCCATTAGAGAGATTCCTAGTTCCTCAAATTGGGAGGAAGTGCAGAAATGGTGGAAGTTCTTCTGGACAAGGGAAATTCCTAGTAAGATAAAATTTTTTGCTTGGAGGCTGTTCCATGACCTCCTGCCAACTGAAACAAATCTAAGGAGGAGGGGGATGGATATTTATCAAGGGTGTCAAAGGTGCAAATTGGAAACGGAGACTACCTTTCATGCCATCTGGGAATGTGTGGAAGTGAAGTGTTGGTGGCGATGCTCGCCGTTTTATCCCCGCTCAGGTCCCCCGAGCATAAGAAGTGTGGCTGACCTGCTGTGGTGGTGCAGGGAGAATTTGTTGAATGAGTGTTTTGAGGAGTTCGTGGTGATGTGCTAG

Coding sequence (CDS)

GGCCTAATAAAGGAATGCAATCGTATAATGGCGAAGTTCTGGTGGGGGGAGGAGGGGGTGAAAAGGAGGATCCATTAGGCCTCGTGGAGGAGAGTGAGGCCCTGTTGGCGAAACAAGGGTGGAGGTTAATAGACAATCCTGATTCCCTTTTGGGGAAAGTTCTTAAGGGTAGGTACTTCTCGAGATGTTCATTTCTAGAGGCCGAAAGGAGAAGCAACGCCTCTTATGTGTGGAGGAGTTTGGTGTGGGGAAGGGGTCTTTTGGAGGAAGGTTTACGGTGGAGGGTGGGAAATGGGAGTTCTGTTAAAATATTGGGGGATAGGTGGATTCCGAGGGCTCCGTCACTGAGGATTCTAATGGGGGAAGGGATTGATCCAGATGCGAGAGTATCAGAGCTTTTGAGTCCTGAAGGGGCATGGAATGTGGATATGGTTCAGGCGATGTTTAAAGGGGATGATGTGCAGGCCATCCTTGGCATTCCTCGTCCTAGAATGGCCTGCCCGGATAGGCTAGTTTGGCATTGTGAGAAGGATGGGATCTACTCGGTGAAGAGTGGGTACAATCTAGCTTGTTCCATTAGAGAGATTCCTAGTTCCTCAAATTGGGAGGAAGTGCAGAAATGGTGGAAGTTCTTCTGGACAAGGGAAATTCCTAGTAAGATAAAATTTTTTGCTTGGAGGCTGTTCCATGACCTCCTGCCAACTGAAACAAATCTAAGGAGGAGGGGGATGGATATTTATCAAGGGTGTCAAAGGTGCAAATTGGAAACGGAGACTACCTTTCATGCCATCTGGGAATGTGTGGAAGTGAAGTGTTGGTGGCGATGCTCGCCGTTTTATCCCCGCTCAGGTCCCCCGAGCATAAGAAGTGTGGCTGACCTGCTGTGGTGGTGCAGGGAGAATTTGTTGAATGAGTGTTTTGAGGAGTTCGTGGTGATGTGCTAG

Protein sequence

PNKGMQSYNGEVLVGGGGGEKEDPLGLVEESEALLAKQGWRLIDNPDSLLGKVLKGRYFSRCSFLEAERRSNASYVWRSLVWGRGLLEEGLRWRVGNGSSVKILGDRWIPRAPSLRILMGEGIDPDARVSELLSPEGAWNVDMVQAMFKGDDVQAILGIPRPRMACPDRLVWHCEKDGIYSVKSGYNLACSIREIPSSSNWEEVQKWWKFFWTREIPSKIKFFAWRLFHDLLPTETNLRRRGMDIYQGCQRCKLETETTFHAIWECVEVKCWWRCSPFYPRSGPPSIRSVADLLWWCRENLLNECFEEFVVMC
Homology
BLAST of Lcy12g004200 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 2.4e-13
Identity = 35/79 (44.30%), Postives = 50/79 (63.29%), Query Frame = 0

Query: 31  SEALLAKQGWRLIDNPDSLLGKVLKGRYFSRCSFLEAERRSNASYVWRSLVWGRGLLEEG 90
           ++ALLAKQ +R+I  P +LL ++L+ RYF   S +E    +  SY WRS++ GR LL  G
Sbjct: 66  NQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGRELLSRG 125

Query: 91  LRWRVGNGSSVKILGDRWI 110
           L   +G+G   K+  DRWI
Sbjct: 126 LLRTIGDGIHTKVWLDRWI 144

BLAST of Lcy12g004200 vs. ExPASy TrEMBL
Match: A0A6J1DAR4 (uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018954 PE=4 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 1.8e-64
Identity = 122/252 (48.41%), Postives = 163/252 (64.68%), Query Frame = 0

Query: 31  SEALLAKQGWRLIDNPDSLLGKVLKGRYFSRCSFLEAERRSNASYVWRSLVWGRGLLEEG 90
           ++ALLAKQ WR++++P+S+L +VLKGRYF  CSF+EA+   N SY+WRS++WGR LL++G
Sbjct: 655 NKALLAKQCWRILNHPNSMLSRVLKGRYFKDCSFMEAKISGNPSYIWRSILWGRDLLKKG 714

Query: 91  LRWRVGNGSSVKILGDRWIPRAPSLRILMGEGIDPDARVSELLS-PEGAWNVDMVQAMFK 150
           LRWR+GNG SV I GD W+P  P+L+IL    +   +RVS L+   EG W  D+V+  F 
Sbjct: 715 LRWRIGNGDSVFIYGDNWVPNQPTLKILSSPRLPLVSRVSSLVDHEEGGWQGDVVRDEFT 774

Query: 151 GDDVQAILGIPRPRMACPDRLVWHCEKDGIYSVKSGYNLAC---SIREIPSSSNWEEVQK 210
            D+ + IL IP  R A  DRL+W+ EK G+YSV+SGY +A       + PSSS+ EEV+ 
Sbjct: 775 PDEAKGILSIPIGRGAEEDRLIWNYEKTGVYSVRSGYKVALLNNPCVQAPSSSSSEEVRC 834

Query: 211 WWKFFWTREIPSKIKFFAWRLFHDLLPTETNLRRRGMDIYQGCQRCKLETETTFHAIWEC 270
           WW  FW   IP+KIK F WRL  D LPT  NL +RG++I   C  C    E + H  W C
Sbjct: 835 WWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLSKRGVEITNCCYFCGRNGEDSIHLFWIC 894

Query: 271 VEVKCWWRCSPF 279
              +  W  S F
Sbjct: 895 KFAEALWINSKF 906

BLAST of Lcy12g004200 vs. ExPASy TrEMBL
Match: A0A803PPQ0 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 5.9e-63
Identity = 113/285 (39.65%), Postives = 168/285 (58.95%), Query Frame = 0

Query: 27  LVEESEALLAKQGWRLIDNPDSLLGKVLKGRYFSRCSFLEAERRSNASYVWRSLVWGRGL 86
           L E ++ALLAKQGWRLI NP SLL +VLK  Y+   SF+EA   S AS VW+ + WGR +
Sbjct: 663 LNEFNQALLAKQGWRLIHNPQSLLARVLKHNYYPNVSFMEAGCPSGASCVWKGICWGRKI 722

Query: 87  LEEGLRWRVGNGSSVKILGDRWIPRAPSLRILMGEGIDPDARVSELLSPEGAWNVDMVQA 146
           L+EG RWR+GNG   ++  D+W+PR     +L    ++P  ++ +L++ +G W +D +Q 
Sbjct: 723 LQEGARWRIGNGREARVWEDKWMPRPSGTILLKKPELEPQTKLHDLINSDGQWQIDKLQK 782

Query: 147 MFKGDDVQAILGIPRPRMACPDRLVWHCEKDGIYSVKSGYNLACSIREIPS-SSNWEEVQ 206
            F  +D+  + GIP   +   D L W    +G Y VKSGY +   I   P+ SSN E++ 
Sbjct: 783 HFHEEDIPWVQGIPID-LYVEDTLTWPYTPNGQYMVKSGYRIGREINLHPTRSSNMEDIH 842

Query: 207 KWWKFFWTREIPSKIKFFAWRLFHDLLPTETNLRRRGMDIYQGCQRCKLETETTFHAIWE 266
           KWWK  W+  +P ++K F WR+ H+ LP + NL  RGMD+   C  C  + ET  HA+W 
Sbjct: 843 KWWKMLWSMSLPPRMKLFGWRVCHNWLPAKINLAHRGMDVNLSCDLCGHQAETLTHALWG 902

Query: 267 CVEVKCWWRCSPFYPRSGPPSIRSVADLLWWCRENLLNECFEEFV 311
           C +VK  W+  P+Y +       S+ D++   +++L    FEE +
Sbjct: 903 CAKVKTIWKLVPWYHKCAHFKNGSMFDIMVTLKDHLHKSEFEEAI 946

BLAST of Lcy12g004200 vs. ExPASy TrEMBL
Match: A0A803Q6D6 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 1.0e-62
Identity = 115/285 (40.35%), Postives = 166/285 (58.25%), Query Frame = 0

Query: 27  LVEESEALLAKQGWRLIDNPDSLLGKVLKGRYFSRCSFLEAERRSNASYVWRSLVWGRGL 86
           L E ++ALLAKQGWRLI NP SLL +VLK  Y+   SF+EA     AS VW+ + WGR +
Sbjct: 76  LNEFNQALLAKQGWRLIYNPQSLLARVLKNSYYPNVSFMEAGCPPGASCVWKGICWGRKI 135

Query: 87  LEEGLRWRVGNGSSVKILGDRWIPRAPSLRILMGEGIDPDARVSELLSPEGAWNVDMVQA 146
           L+EG RWR+GNG   ++  D+WIPR     +L    I+P  ++   ++ +G W +D V+ 
Sbjct: 136 LQEGARWRIGNGREARVWEDKWIPRPSGTILLKKPEIEPQTKLHYFINTDGQWQIDKVKK 195

Query: 147 MFKGDDVQAILGIPRPRMACPDRLVWHCEKDGIYSVKSGYNLACSIREIPS-SSNWEEVQ 206
            F  +D+  + GIP   +   D L W    +G Y VKSGY +   I   P+ SSN E++ 
Sbjct: 196 HFHEEDIPWVQGIPID-LYVEDTLTWPYTPNGQYMVKSGYRIGREINLHPTRSSNMEDIH 255

Query: 207 KWWKFFWTREIPSKIKFFAWRLFHDLLPTETNLRRRGMDIYQGCQRCKLETETTFHAIWE 266
           KWWK  W+  +P ++K F WR+ H+ LP +TNL  RGMD+   C  C  + ET  HA+W 
Sbjct: 256 KWWKMLWSMSLPPRMKLFGWRVCHNWLPAKTNLAHRGMDVNLSCDLCGHQAETLTHALWG 315

Query: 267 CVEVKCWWRCSPFYPRSGPPSIRSVADLLWWCRENLLNECFEEFV 311
           C +VK  W+  P+Y +       S+ D++   +E+L    FEE +
Sbjct: 316 CAKVKTIWKLVPWYNKCAHFKNGSMFDIMVTLKEHLDKSEFEEAI 359

BLAST of Lcy12g004200 vs. ExPASy TrEMBL
Match: A0A803QPB7 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 2.9e-62
Identity = 119/295 (40.34%), Postives = 174/295 (58.98%), Query Frame = 0

Query: 16   GGGGEKEDPLGLVEESEALLAKQGWRLIDNPDSLLGKVLKGRYFSRCSFLEAERRSNASY 75
            GG G ++    L + +++LLAKQGW+LI NPD LL KVLK  YFS  SF EA++    S 
Sbjct: 1201 GGMGFRD----LDDFNQSLLAKQGWKLIQNPDCLLAKVLKALYFSSESFFEAKQGHYGST 1260

Query: 76   VWRSLVWGRGLLEEGLRWRVGNGSSVKILGDRWIPRAPSLRILMGEGIDPDARVSELLSP 135
            VWR ++WGR LL +G RW VGNGS ++I  D+W+PR+    +     I  +  ++ LL P
Sbjct: 1261 VWRGILWGRELLRKGTRWCVGNGSKIRINEDQWLPRSQPFTLRSKVQIPAEVTINSLLQP 1320

Query: 136  EGAWNVDMVQAMFKGDDVQAILGIPRPRMACPDRLVWHCEKDGIYSVKSGYNLACSIREI 195
            +G W  + V++ F  DD+  +LGI +P +   D + W    +GIYSV SGY L      +
Sbjct: 1321 DGNWKENEVRSWFHNDDIPWVLGI-KPAINRSDWITWSPSPNGIYSVASGYKLRFQNPNV 1380

Query: 196  PSSSNWEEVQKWWKFFWTREIPSKIKFFAWRLFHDLLPTETNLRRRGMDIYQGCQRCKLE 255
               SN  +++ WWKF W   +  KIK F WR+F+  +PT+  L +RGM I   C+ C  +
Sbjct: 1381 AECSNKSQIKSWWKFIWGSHLTLKIKNFIWRVFNHWIPTKLELAKRGMVIDTHCELCTFQ 1440

Query: 256  TETTFHAIWECVEVKCWWRCSPFYPRSGPPSIRSVADLLWWCRENLLNECFEEFV 311
             E   HA+W C +V+  W+   F P+  P +++  AD+LWW  E+L  E F +F+
Sbjct: 1441 NEDICHALWHCPKVQNIWKLFGF-PKLIPLNLKKAADVLWWLHEHLPKEEFFKFM 1489

BLAST of Lcy12g004200 vs. ExPASy TrEMBL
Match: A0A803QLY3 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 1.9e-61
Identity = 122/299 (40.80%), Postives = 170/299 (56.86%), Query Frame = 0

Query: 14   VGGGGGEKEDPLG---LVEESEALLAKQGWRLIDNPDSLLGKVLKGRYFSRCSFLEAERR 73
            VG    E++  LG   L E ++ALLAKQGWRLI NP SLL  VLK  YF   SF+EA+  
Sbjct: 830  VGRRKKEEDGGLGFRSLTEFNQALLAKQGWRLIHNPHSLLAGVLKNSYFPTSSFMEAKCP 889

Query: 74   SNASYVWRSLVWGRGLLEEGLRWRVGNGSSVKILGDRWIPRAPSLRILMGEGIDPDARVS 133
              AS+VW+ ++WGR +L EG RWRVGNG ++++  D+W+PR     +     I P  ++ 
Sbjct: 890  PRASWVWKGIIWGRKILSEGARWRVGNGRNIRVWEDKWLPRPSGAILTHKPRIQPITKLQ 949

Query: 134  ELLSPEGAWNVDMVQAMFKGDDVQAILGIPRPRMACPDRLVWHCEKDGIYSVKSGYNLAC 193
              ++ EG W +++V   F  +D+  I GIP   +   D L W    +G Y VKSGY +  
Sbjct: 950  TFITAEGTWKLEVVDRHFHREDIPWIRGIPID-LYTEDILTWPYTSNGNYMVKSGYRVGR 1009

Query: 194  SIREIPS-SSNWEEVQKWWKFFWTREIPSKIKFFAWRLFHDLLPTETNLRRRGMDIYQGC 253
             +   P+  SN  E+ KWWK  W+ ++P  +K F WR+ H+ LPT+TNL  RGM+I   C
Sbjct: 1010 EMNLHPTRCSNMGEINKWWKMLWSLQLPPMMKLFGWRVCHNWLPTKTNLCHRGMNISPVC 1069

Query: 254  QRCKLETETTFHAIWECVEVKCWWRCSPFYPRSGPPSIRSVADLLWWCRENLLNECFEE 309
              C    ET  HA+W C +VK  W+  P+Y   G     S+ DLL   +E L    FE+
Sbjct: 1070 DLCGSHVETLPHALWNCDKVKTVWKLLPWYKHCGKLGDGSMFDLLTALKEKLTRSEFED 1127

BLAST of Lcy12g004200 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 256.1 bits (653), Expect = 3.8e-64
Identity = 122/252 (48.41%), Postives = 163/252 (64.68%), Query Frame = 0

Query: 31  SEALLAKQGWRLIDNPDSLLGKVLKGRYFSRCSFLEAERRSNASYVWRSLVWGRGLLEEG 90
           ++ALLAKQ WR++++P+S+L +VLKGRYF  CSF+EA+   N SY+WRS++WGR LL++G
Sbjct: 655 NKALLAKQCWRILNHPNSMLSRVLKGRYFKDCSFMEAKISGNPSYIWRSILWGRDLLKKG 714

Query: 91  LRWRVGNGSSVKILGDRWIPRAPSLRILMGEGIDPDARVSELLS-PEGAWNVDMVQAMFK 150
           LRWR+GNG SV I GD W+P  P+L+IL    +   +RVS L+   EG W  D+V+  F 
Sbjct: 715 LRWRIGNGDSVFIYGDNWVPNQPTLKILSSPRLPLVSRVSSLVDHEEGGWQGDVVRDEFT 774

Query: 151 GDDVQAILGIPRPRMACPDRLVWHCEKDGIYSVKSGYNLAC---SIREIPSSSNWEEVQK 210
            D+ + IL IP  R A  DRL+W+ EK G+YSV+SGY +A       + PSSS+ EEV+ 
Sbjct: 775 PDEAKGILSIPIGRGAEEDRLIWNYEKTGVYSVRSGYKVALLNNPCVQAPSSSSSEEVRC 834

Query: 211 WWKFFWTREIPSKIKFFAWRLFHDLLPTETNLRRRGMDIYQGCQRCKLETETTFHAIWEC 270
           WW  FW   IP+KIK F WRL  D LPT  NL +RG++I   C  C    E + H  W C
Sbjct: 835 WWNGFWKMHIPNKIKVFLWRLCLDRLPTGCNLSKRGVEITNCCYFCGRNGEDSIHLFWIC 894

Query: 271 VEVKCWWRCSPF 279
              +  W  S F
Sbjct: 895 KFAEALWINSKF 906

BLAST of Lcy12g004200 vs. NCBI nr
Match: XP_030483251.1 (uncharacterized protein LOC115699848 [Cannabis sativa])

HSP 1 Score: 246.9 bits (629), Expect = 2.3e-61
Identity = 117/283 (41.34%), Postives = 163/283 (57.60%), Query Frame = 0

Query: 27  LVEESEALLAKQGWRLIDNPDSLLGKVLKGRYFSRCSFLEAERRSNASYVWRSLVWGRGL 86
           L E ++ALLAKQGWRLI NP SLL  VLK  YF   SF+EA+    AS+VW+ ++WGR +
Sbjct: 225 LTEFNQALLAKQGWRLIHNPHSLLAGVLKNSYFPTSSFMEAKCPPRASWVWKGIIWGRKI 284

Query: 87  LEEGLRWRVGNGSSVKILGDRWIPRAPSLRILMGEGIDPDARVSELLSPEGAWNVDMVQA 146
           L EG RWRVGNG ++++  D+W+PR     +     I P  ++   ++ EG W +++V  
Sbjct: 285 LSEGARWRVGNGRNIRVWEDKWLPRPSGAILTHKPRIQPITKLQTFITAEGTWKLEVVDR 344

Query: 147 MFKGDDVQAILGIPRPRMACPDRLVWHCEKDGIYSVKSGYNLACSIREIPS-SSNWEEVQ 206
            F  +D+  I GIP   +   D L W    +G Y VKSGY +   +   P+  SN  E+ 
Sbjct: 345 HFHREDIPWIRGIPID-LYTEDILTWPYTSNGNYMVKSGYRVGREMNLHPTRCSNMGEIN 404

Query: 207 KWWKFFWTREIPSKIKFFAWRLFHDLLPTETNLRRRGMDIYQGCQRCKLETETTFHAIWE 266
           KWWK  W+ ++P  +K F WR+ H+ LPT+TNL  RGM+I   C  C    ET  HA+W 
Sbjct: 405 KWWKMLWSLQLPPMMKLFGWRVCHNWLPTKTNLCHRGMNISPVCDLCGSHVETLPHALWN 464

Query: 267 CVEVKCWWRCSPFYPRSGPPSIRSVADLLWWCRENLLNECFEE 309
           C +VK  W+  P+Y   G     S+ DLL   +E L    FE+
Sbjct: 465 CDKVKTVWKLLPWYKHCGKLGDGSMFDLLTALKEKLTRSEFED 506

BLAST of Lcy12g004200 vs. NCBI nr
Match: XP_030505362.1 (uncharacterized protein LOC115720349 [Cannabis sativa])

HSP 1 Score: 240.0 bits (611), Expect = 2.8e-59
Identity = 116/285 (40.70%), Postives = 164/285 (57.54%), Query Frame = 0

Query: 27  LVEESEALLAKQGWRLIDNPDSLLGKVLKGRYFSRCSFLEAERRSNASYVWRSLVWGRGL 86
           L E ++ALLAKQGWRLI +P SLL +VLK  Y+   SFL+A+ +  AS +W+ + WGR +
Sbjct: 439 LTEFNQALLAKQGWRLIHHPHSLLARVLKHSYYPNSSFLQAKCQPGASSIWQGITWGRQI 498

Query: 87  LEEGLRWRVGNGSSVKILGDRWIPRAPSLRILMGEGIDPDARVSELLSPEGAWNVDMVQA 146
           + EG RWRVGNG +V+I  D+WIPR           + P+  + +LL+ EG W  DM+ A
Sbjct: 499 IVEGARWRVGNGRTVRIWEDKWIPRPNDTITYRHTDLAPNTTIHKLLTEEGDWKTDMIHA 558

Query: 147 MFKGDDVQAILGIPRPRMACPDRLVWHCEKDGIYSVKSGYNLACSIREIPS-SSNWEEVQ 206
            F  DD+  ILG P   +   D L W     G Y VKSGY +A  +   P+  SN ++++
Sbjct: 559 YFHKDDIPWILGTP-VDIQEEDVLTWPLSSKGHYLVKSGYRVARELNVCPTRCSNMDQIK 618

Query: 207 KWWKFFWTREIPSKIKFFAWRLFHDLLPTETNLRRRGMDIYQGCQRCKLETETTFHAIWE 266
            WWK +W  ++P +IK F W+L H+ LP ++NL  RGM I   C  C    E+  HAIW 
Sbjct: 619 AWWKMWWHLQLPPRIKLFGWKLCHNWLPAKSNLIHRGMQIDPVCHLCGTYVESLPHAIWS 678

Query: 267 CVEVKCWWRCSPFYPRSGPPSIRSVADLLWWCRENLLNECFEEFV 311
           C + K  W+  P+Y R       S+ D L   + +L  + FEE +
Sbjct: 679 CAKAKEVWKLLPYYSRICKYKGGSMFDFLVEIKHHLSIDEFEEVI 722

BLAST of Lcy12g004200 vs. NCBI nr
Match: XP_024037590.1 (uncharacterized protein LOC112097210 [Citrus clementina])

HSP 1 Score: 233.0 bits (593), Expect = 3.4e-57
Identity = 107/248 (43.15%), Postives = 150/248 (60.48%), Query Frame = 0

Query: 31  SEALLAKQGWRLIDNPDSLLGKVLKGRYFSRCSFLEAERRSNASYVWRSLVWGRGLLEEG 90
           ++AL+AKQGWR++  P SL+ +VLK RYF    F+ A   S  S+VWRS+VWGR +L +G
Sbjct: 661 NQALVAKQGWRIMQFPSSLVARVLKARYFKHTGFMNAGLGSKPSFVWRSIVWGRQVLHKG 720

Query: 91  LRWRVGNGSSVKILGDRWIPRAPSLRILMGEGIDPDARVSELLSPEGAWNVDMVQAMFKG 150
            RWR+GNG +V + G+ WIPR  + + +    +  D  V+EL+  +  W  D++   F+ 
Sbjct: 721 ARWRIGNGQNVLVYGNNWIPRPTTFKPISAPSMGTDTTVAELIDEKQQWREDLILQHFRP 780

Query: 151 DDVQAILGIPRPRMACPDRLVWHCEKDGIYSVKSGYNLACSIR--EIPSSSNWEEVQKWW 210
           +D +AI+ IP P+    D+L+WH +K G YSVKSGY +A  I+  E PS SN +  Q  W
Sbjct: 781 EDAEAIMQIPLPKRPKEDQLIWHYDKKGYYSVKSGYQVAMRIKFPEDPSCSNHD--QNLW 840

Query: 211 KFFWTREIPSKIKFFAWRLFHDLLPTETNLRRRGMDIYQGCQRCKLETETTFHAIWECVE 270
           +F W   IP K+K F WR  HDLLPT  NL ++ +     CQ C    ET  HA+ EC  
Sbjct: 841 RFIWKLAIPEKVKIFLWRAAHDLLPTAENLWKKKVLQEPMCQSCHCHVETVSHALVECNR 900

Query: 271 VKCWWRCS 277
            +  WR S
Sbjct: 901 ARKIWRYS 906

BLAST of Lcy12g004200 vs. NCBI nr
Match: XP_024950112.1 (uncharacterized protein LOC112496847 [Citrus sinensis])

HSP 1 Score: 227.3 bits (578), Expect = 1.9e-55
Identity = 107/283 (37.81%), Postives = 160/283 (56.54%), Query Frame = 0

Query: 31   SEALLAKQGWRLIDNPDSLLGKVLKGRYFSRCSFLEAERRSNASYVWRSLVWGRGLLEEG 90
            ++AL+AKQ WRL+  P+SL+ +VL+ RYF   SFL A+  +NASY+WRS++WGR ++++G
Sbjct: 897  NQALVAKQAWRLLQYPNSLVSRVLQARYFRNSSFLCAKAGANASYIWRSIMWGRQVIKKG 956

Query: 91   LRWRVGNGSSVKILGDRWIPRAPSLRILMGEGIDPDARVSELLSPEGAWNVDMVQAMFKG 150
            +RWR+GNG  + I  D W+PR  + R +    +   + V++L+  +  W+   ++  F  
Sbjct: 957  MRWRIGNGKKIAIFSDNWLPRPETFRPIFPLSLPVSSVVADLIKADNQWDEIKLRQHFLD 1016

Query: 151  DDVQAILGIPRPRMACPDRLVWHCEKDGIYSVKSGYNLACSIREIPSSSNWEEVQKWWKF 210
             D   IL IP P     D ++WH +K G YSVKSGY LA   +   S+S  E   K+W  
Sbjct: 1017 VDTAEILKIPLPAEKAEDEVLWHYDKRGNYSVKSGYQLALRSKFPDSTSCTEASHKYWSA 1076

Query: 211  FWTREIPSKIKFFAWRLFHDLLPTETNLRRRGMDIYQGCQRCKLETETTFHAIWECVEVK 270
             WT E+P K+K F WR  ++LLP+  NL +R +     C+RCKL  ET  HA+ EC   +
Sbjct: 1077 LWTLELPEKLKIFMWRASNNLLPSAENLWKRKVVEEPTCKRCKLSVETISHALLECKAAR 1136

Query: 271  CWWRCSPFYPRSGPPSIRSVADLLWWCRENLLNECFEEFVVMC 314
              W  SPF       + + +   L    + L     E  V +C
Sbjct: 1137 KIWLQSPFSAPRLEANSQDIFSTLQNMAKELRKSDLELMVALC 1179

BLAST of Lcy12g004200 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 130.2 bits (326), Expect = 2.9e-30
Identity = 79/277 (28.52%), Postives = 124/277 (44.77%), Query Frame = 0

Query: 33  ALLAKQGWRLIDNPDSLLGKVLKGRYFSRCSFLEAERRSNASYVWRSLVWGRGLLEEGLR 92
           ALL KQ WR++  P+SL+ KV K RYF +   L A   S  S+VW+S+   + +L +G R
Sbjct: 67  ALLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLNAPLGSRPSFVWKSIHASQEILRQGAR 126

Query: 93  WRVGNGSSVKILGDRWIPRAPSLRILMGEGIDPD--------ARVSELLSPEG-AWNVDM 152
             VGNG  + I   +W+   P+   L  + + P          +VS+L+   G  W  D+
Sbjct: 127 AVVGNGEDIIIWRHKWLDSKPASAALRMQRVPPQEYASVSSILKVSDLIDESGREWRKDV 186

Query: 153 VQAMFKGDDVQAILGIPRP-RMACPDRLVWHCEKDGIYSVKSGYNLACSI---REIPSSS 212
           ++ +F  +  + ++G  RP      D   W     G Y+VKSGY +   I   R  P   
Sbjct: 187 IEMLFP-EVERKLIGELRPGGRRILDSYTWDYTSSGDYTVKSGYWVLTQIINKRSSPQEV 246

Query: 213 NWEEVQKWWKFFWTREIPSKIKFFAWRLFHDLLPTETNLRRRGMDIYQGCQRCKLETETT 272
           +   +   ++  W  +   KI+ F W+   + LP    L  R +     C RC    ET 
Sbjct: 247 SEPSLNPIYQKIWKSQTSPKIQHFLWKCLSNSLPVAGALAYRHLSKESACIRCPSCKETV 306

Query: 273 FHAIWECVEVKCWWRCSPFYPRSGPPSIRSVADLLWW 297
            H +++C   +  W  S      G     S+   L+W
Sbjct: 307 NHLLFKCTFARLTWAISSIPIPLGGEWADSIYVNLYW 342

BLAST of Lcy12g004200 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 77.8 bits (190), Expect = 1.7e-14
Identity = 35/79 (44.30%), Postives = 50/79 (63.29%), Query Frame = 0

Query: 31  SEALLAKQGWRLIDNPDSLLGKVLKGRYFSRCSFLEAERRSNASYVWRSLVWGRGLLEEG 90
           ++ALLAKQ +R+I  P +LL ++L+ RYF   S +E    +  SY WRS++ GR LL  G
Sbjct: 66  NQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGRELLSRG 125

Query: 91  LRWRVGNGSSVKILGDRWI 110
           L   +G+G   K+  DRWI
Sbjct: 126 LLRTIGDGIHTKVWLDRWI 144

BLAST of Lcy12g004200 vs. TAIR 10
Match: AT2G02650.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 57.8 bits (138), Expect = 1.8e-08
Identity = 29/77 (37.66%), Postives = 37/77 (48.05%), Query Frame = 0

Query: 212 WTREIPSKIKFFAWRLFHDLLPTETNLRRRGMDIYQGCQRCKLETETTFHAIWECVEVKC 271
           W   +  KIK F WR     L T T LR R +D    CQRC +E ET  H ++ C   + 
Sbjct: 38  WKLHVAPKIKHFLWRCVTGALATNTRLRSRNIDADPICQRCCIEEETIHHIMFNCPYTQS 97

Query: 272 WWRCSPFY--PRSGPPS 287
            WR +      + GPPS
Sbjct: 98  VWRSANIIIGNQWGPPS 114

BLAST of Lcy12g004200 vs. TAIR 10
Match: AT2G22440.1 (BEST Arabidopsis thaliana protein match is: Ribonuclease H-like superfamily protein (TAIR:AT4G29090.1); Has 208 Blast hits to 191 proteins in 10 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 208; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 47.0 bits (110), Expect = 3.3e-05
Identity = 31/92 (33.70%), Postives = 43/92 (46.74%), Query Frame = 0

Query: 102 KILGDRWIP---RAPSLRILMGEGIDPDARVSELLSPE-GAWNVDMVQAMFKGDDVQAIL 161
           ++  D WIP     P+  IL     D    V++L+      W +D +QA+    D+  IL
Sbjct: 4   RVWKDPWIPTILARPAKSILNIR--DSLLYVNDLIDQNTNLWKLDRLQALIDPVDIPLIL 63

Query: 162 GIPRPRMACPDRLVWHCEKDGIYSVKSGYNLA 190
           GI   R    D   W   K G Y+VKSGY +A
Sbjct: 64  GIRPSRTYLSDGFSWSHTKSGNYTVKSGYWVA 93

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P932952.4e-1344.30Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1DAR41.8e-6448.41uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A803PPQ05.9e-6339.65Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803Q6D61.0e-6240.35Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803QPB72.9e-6240.34Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803QLY31.9e-6140.80Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022150918.13.8e-6448.41uncharacterized protein LOC111018954 [Momordica charantia][more]
XP_030483251.12.3e-6141.34uncharacterized protein LOC115699848 [Cannabis sativa][more]
XP_030505362.12.8e-5940.70uncharacterized protein LOC115720349 [Cannabis sativa][more]
XP_024037590.13.4e-5743.15uncharacterized protein LOC112097210 [Citrus clementina][more]
XP_024950112.11.9e-5537.81uncharacterized protein LOC112496847 [Citrus sinensis][more]
Match NameE-valueIdentityDescription
AT4G29090.12.9e-3028.52Ribonuclease H-like superfamily protein [more]
ATMG00310.11.7e-1444.30RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT2G02650.11.8e-0837.66Ribonuclease H-like superfamily protein [more]
AT2G22440.13.3e-0533.70BEST Arabidopsis thaliana protein match is: Ribonuclease H-like superfamily prot... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 180..272
e-value: 1.9E-18
score: 66.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availablePANTHERPTHR33116:SF41SUBFAMILY NOT NAMEDcoord: 27..213
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 27..213

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy12g004200.1Lcy12g004200.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003824 catalytic activity