Lcy12g002500 (gene) Sponge gourd (P93075) v1

BLAST of Lcy12g002500 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 1.8e-162
Identity = 389/986 (39.45%), Postives = 546/986 (55.38%), Query Frame = 0

Query: 4   SSSCSSSGKWKYDVFLSFRGEDTRGGFTDHLYKALVRKGIFTFRDEDEIKEGKGISSDLL 63
           SS+ S      YDVFLSFRGEDTR  FTDHLY AL R+GI TFRD D ++ G+ I+ +LL
Sbjct: 13  SSTPSIPRTTTYDVFLSFRGEDTRYNFTDHLYSALGRRGIRTFRD-DRLRRGEAIAPELL 72

Query: 64  AAIEASRFALVVVSQNYASSRWCLEELVKIFDCQGRLRMSVLPVFYKVDPSDVRNQRGSF 123
            AIE SR +++V S+NYA SRWCL+ELVKI +CQ  L  +V P+FY VDPS VR Q GSF
Sbjct: 73  KAIEESRSSVIVFSENYAHSRWCLDELVKIMECQKDLGHAVFPIFYHVDPSHVRKQEGSF 132

Query: 124 GEAFVKHELRFGEDDSKIRKWRQALTKLANLKAWLSQPWSHESKIIEEITEAIWKRIKPT 183
           GEAF  +E  + +   KI +WR ALT+ ANL  W      +ES  I+EIT +I++++K  
Sbjct: 133 GEAFAGYEENWKD---KIPRWRTALTEAANLSGWHLLDDRYESNQIKEITNSIFRQLK-C 192

Query: 184 LTVTQEDQLVGINSKLNKLFSLLNPNLVDDVVCVGIHGMGGIGKTTIARVCYERIRDEFE 243
             +     LVGI+S + ++   L+     DV  VGI+G+GGIGKTTIA+V Y  +  EFE
Sbjct: 193 KRLDVGANLVGIDSHVKEMILRLHLE-SSDVRMVGIYGVGGIGKTTIAKVIYNELSCEFE 252

Query: 244 AHCFLSDVREKFETSGLPHLQSKLLSRMFSIK-NNDIWDVEEGIAMINRAIFQKKTLLVL 303
              FL ++RE      L HLQ++LL  +   + + +I  V    +MI   +  ++  +VL
Sbjct: 253 YMSFLENIREGSNPQVLFHLQNQLLGDILEGEGSQNISSVAHRASMIKDILLSRRVFIVL 312

Query: 304 DDVDSSDQITGLIPNKGSFGSGSRIIITTRNADLLSNEFEVKRIFEVEELQYEEALQLLN 363
           DDVD   Q+  L+ ++   G GSR+IITTRN  +L+ + EV  ++EVE L +EEA +L +
Sbjct: 313 DDVDDLSQLEYLLGHREWLGEGSRVIITTRNKHVLAVQ-EVDDLYEVEGLNFEEACELFS 372

Query: 364 LSAFTKTCPKECFLEHSKSIVKVVGGHPLALKLLGSSLRNKDLSVWNDVIEELQGGGNIH 423
           L AF +  PK  +   +  +V    G PLALK+LGS L  K +  W   +++L       
Sbjct: 373 LYAFKQNLPKSDYRNLTCRVVGYCQGLPLALKVLGSLLCKKTIPQWEGELKKLDSEPKA- 432

Query: 424 DKIFKCLKVSYDGLDEWEKEIFLDVACFFKGKRRELVEEMLNGCGFYAKRRVELLIQKSL 483
             I K LK SYDGLD  +K IFLD+ACFFKG+ R+ V  +L+GC F A+  +  L    L
Sbjct: 433 -DIHKVLKRSYDGLDRIDKNIFLDLACFFKGEGRDFVLRILDGCDFPAETGISNLNDLCL 492

Query: 484 LTLSYDNRLHMHDLLQEMGRKIVRHK-PIQ----DRLWCHKDIKSV------VREALVQS 543
           +TL Y N++ MHDL+Q+MG +IVR   P++     RLW   D +        ++     S
Sbjct: 493 ITLPY-NQICMHDLIQQMGWEIVRENFPVEPNKWSRLWDPCDFERALTADEGIKSVETMS 552

Query: 544 IFFKSTRIVVEFPIQFSKMHQLRLLNFKN--------------------------VRLKS 603
           +     + V      F+KM +LRLL   +                           +++ 
Sbjct: 553 LDLSKLKRVCSNSNVFAKMTKLRLLKVYSSSDIDSAHGDSDEDIEEVYDVVMKDASKMQL 612

Query: 604 QLEYCIPT-ELRYLKWKGYPMEFLPLSSEEYKLIGLHMCHSNLKQFWQGEKHLEELKYIK 663
              +  P+ ELRYL+W GYP++ LPL+ +  KL+ LH+  SN+KQ WQG K LE LK I 
Sbjct: 613 GQSFKFPSYELRYLRWDGYPLDSLPLNFDGGKLVELHLKCSNIKQLWQGHKDLERLKVID 672

Query: 664 LNHSQKLSKTPDFTKIPNLERLELEGCTSLVNIHPSIFLAEKLIFLSLKDCINLTNLPRD 723
           L++S+KLS+  +F+ +PNLERL L GC SL++IHPS+   +KL  LSL+ C  L NLP  
Sbjct: 673 LSYSRKLSQMSEFSSMPNLERLCLSGCVSLIDIHPSVGNMKKLTTLSLRSCNKLKNLPDS 732

Query: 724 I-NIKVLQVLILSGCSKVNNIPEFSGNTNRLLQLHLDGTSISNLPPSIATLTHLKVLSLK 783
           I +++ L+ L LS CSK    PE  GN   L +L L  T+I +LP SI  L  L+ L L 
Sbjct: 733 IGDLESLESLYLSNCSKFEKFPEKGGNMKSLTELDLKNTAIKDLPDSIGDLESLESLYLS 792

Query: 784 NCSKLINIPNAIDKMKALQSLNLSGCSNLANRKRKASEAELEELDIRGI---ARRRRRRD 843
           NCSK    P     MK+L  L+L    N A +    S  +LE L+I  +   A+  +  +
Sbjct: 793 NCSKFEKFPEKGGNMKSLTELDL---KNTAIKDLPDSIGDLESLEILNLSDCAKFEKFPE 852

Query: 844 DNDNDNQLVGLNIFRKLFFWLCKAPASCIFGIP-SLAGLYSLTRLNLRDCN-LEVIPRGI 903
              N   L  L++             + I  +P S+  L SL  L+L DC+  E  P   
Sbjct: 853 KGGNMKSLKELDL-----------QNTAIKDLPDSIGDLKSLKYLSLSDCSKFEKFPEKG 912

Query: 904 ECLVSLTELNLCGNNFSHLPTSISQLHNLKRLNINQCKKLVHFPELPPRILRLLSKDC-- 943
             +  L +L L       LP SI  L +LK L ++ C K   FPE    +  L   D   
Sbjct: 913 GNMKRLLQLILSNTAIKDLPDSIGDLESLKYLYLSDCSKFEKFPEKGGNMKSLTELDLKN 972

BLAST of Lcy12g002500 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 2.8e-160
Identity = 395/996 (39.66%), Postives = 553/996 (55.52%), Query Frame = 0

Query: 4   SSSCSSSG------KWKYDVFLSFRGEDTRGGFTDHLYKALVRKGIFTFRDEDEIKEGKG 63
           SSS SSS          YDVFLSFRGEDTR  FTDHLY AL R+GI TFRD D+++ G+ 
Sbjct: 10  SSSSSSSSTPSIPRTITYDVFLSFRGEDTRFNFTDHLYSALGRRGIRTFRD-DKLRRGEA 69

Query: 64  ISSDLLAAIEASRFALVVVSQNYASSRWCLEELVKIFDCQGRLR---MSVLPVFYKVDPS 123
           I+ +LL AIE SR +++V S+NYA SRWCL+ELVKI +C    +    +V P+FY VDPS
Sbjct: 70  IAPELLKAIEESRSSVIVFSENYARSRWCLDELVKIMECHKDKKDPGHAVFPIFYHVDPS 129

Query: 124 DVRNQRGSFGEAFVKHELRFGED-DSKIRKWRQALTKLANLKAWLSQPWSHESKIIEEIT 183
            VR Q GSFGEAF      +GE+   KI +WR ALT+ ANL  W  Q   +ES  I+EIT
Sbjct: 130 HVRKQEGSFGEAFA----GYGENLKDKIPRWRTALTEAANLSGWPLQD-GYESNQIKEIT 189

Query: 184 EAIWKRIKPTLTVTQEDQLVGINSKLNKLFSLLNPNLVDDVVCVGIHGMGGIGKTTIARV 243
           ++I++R+K    +     LVGI+S + ++   L+     DV  VG++G+GGIGKTTIA+V
Sbjct: 190 DSIFRRLK-CKRLDAGANLVGIDSHVKEMIWRLHME-SSDVRMVGMYGVGGIGKTTIAKV 249

Query: 244 CYERIRDEFEAHCFLSDVREKFETSGLPHLQSKLLSRMFSIK-NNDIWDVEEGIAMINRA 303
            Y  +  EFE   FL ++REKF T G+  LQ++LL  +   + + +I  V  G +MI   
Sbjct: 250 IYNELSREFEYMSFLENIREKFNTQGVSPLQNQLLDDILKGEGSQNINSVAHGASMIKDI 309

Query: 304 IFQKKTLLVLDDVDSSDQITGLIPNKGSFGSGSRIIITTRNADLLSNEFEVKRIFEVEEL 363
           +  K   +VLDDVD   Q+  L+ ++   G GSR+IITTRN  +L  + +V  ++EV+ L
Sbjct: 310 LSSKIVFIVLDDVDDQSQLEYLLRHREWLGEGSRVIITTRNKHVLDVQ-KVDDLYEVKGL 369

Query: 364 QYEEALQLLNLSAFTKTCPKECFLEHSKSIVKVVGGHPLALKLLGSSLRNKDLSVWNDVI 423
            +EEA +L +L AF +  PK  +   S  +V    G PLALK+LG  L  K +  W   +
Sbjct: 370 NFEEACELFSLYAFEQNLPKSDYRNLSHRVVGYCQGLPLALKVLGCLLLKKTIPEWESEL 429

Query: 424 EELQGGGNIHDKIFKCLKVSYDGLDEWEKEIFLDVACFFKGKRRELVEEMLNGCGFYAKR 483
            +L        +I   LK SYDGL   EK IFLDVACFFKG+ R+ V ++L+ C F+A+ 
Sbjct: 430 RKLD--REPEAEILSVLKRSYDGLGRTEKSIFLDVACFFKGEDRDFVSKILDACDFHAEI 489

Query: 484 RVELLIQKSLLTLSYDNRLHMHDLLQEMGRKIVRHK-PIQ----DRLWCHKDIKSV---- 543
            ++ L  K L+TL Y NR+ MHDL+Q+MG +IVR K P +     RLW   D +      
Sbjct: 490 GIKNLNDKCLITLQY-NRIRMHDLIQQMGWEIVREKFPDEPNKWSRLWDTCDFERALTAY 549

Query: 544 --VREALVQSIFFKSTRIVVEFPIQFSKMHQLRLLN------------------------ 603
             ++     S+     + V      F+KM +LRLL                         
Sbjct: 550 KGIKRVETISLDLSKLKRVCSNSNAFAKMTRLRLLKVQSSLDIDFEPEYIDADDKVELYD 609

Query: 604 --FKNV-RLKSQLEYCIPT-ELRYLKWKGYPMEFLPLSSEEYKLIGLHMCHSNLKQFWQG 663
              KN  +++    +  P+ ELRYL+W GYP++FLP + +  KL+ LH+  SN+KQ   G
Sbjct: 610 VVMKNASKMRLGRGFKFPSYELRYLRWDGYPLDFLPSNFDGGKLVELHLKCSNIKQLRLG 669

Query: 664 EKHLEELKYIKLNHSQKLSKTPDFTKIPNLERLELEGCTSLVNIHPSIFLAEKLIFLSLK 723
            K LE LK I L++S+KLS+  +F+ +PNLERL L GC SL++IHPS+   +KL  LSLK
Sbjct: 670 NKDLEMLKVIDLSYSRKLSQMSEFSSMPNLERLFLRGCVSLIDIHPSVGNMKKLTTLSLK 729

Query: 724 DCINLTNLPRDI-NIKVLQVLILSGCSKVNNIPEFSGNTNRLLQLHLDGTSISNLPPSIA 783
            C  L NLP  I +++ L++L L+ CSK    PE  GN   L +L L  T+I +LP SI 
Sbjct: 730 SCKKLKNLPDSIGDLESLEILDLAYCSKFEKFPEKGGNMKSLTELDLQNTAIKDLPDSIG 789

Query: 784 TLTHLKVLSLKNCSKLINIPNAIDKMKALQSLNLSGCSNLANRKRKASEAELEELD---I 843
            L  LK L L +CSK    P     MK+L+ L+L    N A +    S  +LE L+   +
Sbjct: 790 DLESLKYLDLSDCSKFEKFPEKGGNMKSLRELDL---RNTAIKDLPDSIRDLESLERLYL 849

Query: 844 RGIARRRRRRDDNDNDNQLVGLNIFRKLFFWLCKAPASCIFGIP-SLAGLYSLTRLNLRD 903
              ++  +  +   N   L+ L++             + I  +P S+  L SL  L+L +
Sbjct: 850 SYCSKFEKFPEKGGNMKSLMELDL-----------QNTAIKDLPDSIGDLESLKYLDLSN 909

Query: 904 CN-LEVIPRGIECLVSLTELNLCGNNFSHLPTSISQLHNLKRLNINQCKKLVHFPELPPR 942
           C+  E  P     + SLTEL L       LP SI  L +L  LN++ C K   FPE    
Sbjct: 910 CSKFEKFPEKGGNMKSLTELFLENTAIKDLPDSIGDLESLVSLNLSDCSKFEKFPEKGGN 969

BLAST of Lcy12g002500 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 2.4e-159
Identity = 396/1085 (36.50%), Postives = 590/1085 (54.38%), Query Frame = 0

Query: 5    SSCSSSGKWKYDVFLSFRGEDTRGGFTDHLYKALVRKGIFTFRDEDEIKEGKGISSDLLA 64
            +S SSS +W YDVFLSFRGEDTR  FT HLY+ L  KGI TF+D+  ++ G  I  +L  
Sbjct: 2    ASSSSSSRWSYDVFLSFRGEDTRKTFTSHLYEVLNDKGIKTFQDDKRLEYGATIPGELCK 61

Query: 65   AIEASRFALVVVSQNYASSRWCLEELVKIFDCQGRLRMSVLPVFYKVDPSDVRNQRGSFG 124
            AIE S+FA+VV S+NYA+SRWCL ELVKI +C+ R + +V+P+FY VDPS VRNQ+ SF 
Sbjct: 62   AIEESQFAIVVFSENYATSRWCLNELVKIMECKTRFKQTVIPIFYDVDPSHVRNQKESFA 121

Query: 125  EAFVKHELRFGEDDSKIRKWRQALTKLANLKAWLSQPWSHESKIIEEITEAIWKRI-KPT 184
            +AF +HE ++ +D   I++WR AL + ANLK         ++  I +I + I  ++ K +
Sbjct: 122  KAFEEHETKYKDDVEGIQRWRIALNEAANLKGSCDNRDKTDADCIRQIVDQISSKLCKIS 181

Query: 185  LTVTQEDQLVGINSKLNKLFSLLNPNLVDDVVCVGIHGMGGIGKTTIARVCYERIRD--- 244
            L+  Q   +VGI++ L K+ SLL    ++ V  +GI GMGG+GKTTIAR  ++ +     
Sbjct: 182  LSYLQ--NIVGIDTHLEKIESLLEIG-INGVRIMGIWGMGGVGKTTIARAIFDTLLGRMD 241

Query: 245  ---EFEAHCFLSDVREKFETSGLPHLQSKLLSRMFSIKNNDIWDVEEGIAMINRAIFQKK 304
               +F+  CFL D++E     G+  LQ+ LLS +   K N   + E+G   +   +  KK
Sbjct: 242  SSYQFDGACFLKDIKE--NKRGMHSLQNALLSELLREKAN-YNNEEDGKHQMASRLRSKK 301

Query: 305  TLLVLDDVDSSDQ-ITGLIPNKGSFGSGSRIIITTRNADLLSNEFEVKRIFEVEELQYEE 364
             L+VLDD+D+ D  +  L  +   FG+GSRIIITTR+  L+        I+EV  L   E
Sbjct: 302  VLIVLDDIDNKDHYLEYLAGDLDWFGNGSRIIITTRDKHLIEKN---DIIYEVTALPDHE 361

Query: 365  ALQLLNLSAFTKTCPKECFLEHSKSIVKVVGGHPLALKLLGSSLRNKDLSVWNDVIEELQ 424
            ++QL    AF K  P E F + S  +V    G PLALK+ GS L N  L+ W   IE ++
Sbjct: 362  SIQLFKQHAFGKEVPNENFEKLSLEVVNYAKGLPLALKVWGSLLHNLRLTEWKSAIEHMK 421

Query: 425  GGGNIHDKIFKCLKVSYDGLDEWEKEIFLDVACFFKGKRRELVEEMLNGCGFYAKRRVEL 484
               N +  I   LK+SYDGL+  ++E+FLD+ACF +G+ ++ + ++L  C   A+  + +
Sbjct: 422  --NNSYSGIIDKLKISYDGLEPKQQEMFLDIACFLRGEEKDYILQILESCHIGAEYGLRI 481

Query: 485  LIQKSLLTLSYDNRLHMHDLLQEMGRKIVRHKP---IQDRLWCHKDIKSVVRE----ALV 544
            LI KSL+ +S  N++ MHDL+Q+MG+ IV  +     + RLW  K+++ V+        +
Sbjct: 482  LIDKSLVFISEYNQVQMHDLIQDMGKYIVNFQKDPGERSRLWLAKEVEEVMSNNTGTMAM 541

Query: 545  QSIFFKSTRIVVEFPIQFSK-MHQLRLLNFKNVRLKSQLEYCIPTELRYLKWKGYPMEFL 604
            ++I+  S    + F  Q  K M +LR+ N         ++Y +P  LR      YP E  
Sbjct: 542  EAIWVSSYSSTLRFSNQAVKNMKRLRVFNMGRSSTHYAIDY-LPNNLRCFVCTNYPWESF 601

Query: 605  PLSSEEYKLIGLHMCHSNLKQFWQGEKHLEELKYIKLNHSQKLSKTPDFTKIPNLERLEL 664
            P + E   L+ L + H++L+  W   KHL  L+ I L+ S++L++TPDFT +PNLE + L
Sbjct: 602  PSTFELKMLVHLQLRHNSLRHLWTETKHLPSLRRIDLSWSKRLTRTPDFTGMPNLEYVNL 661

Query: 665  EGCTSLVNIHPSIFLAEKLIFLSLKDCINLTNLPRDINIKVLQVLILSGCSKVNNIPEFS 724
              C++L  +H S+    K+I L L DC +L   P  +N++ L+ L L  C  +  +PE  
Sbjct: 662  YQCSNLEEVHHSLGCCSKVIGLYLNDCKSLKRFP-CVNVESLEYLGLRSCDSLEKLPEIY 721

Query: 725  GNTNRLLQLHLDGTSISNLPPSIATL-THLKVLSLKNCSKLINIPNAIDKMKALQSLNLS 784
            G     +Q+H+ G+ I  LP SI    TH+  L L N   L+ +P++I ++K+L SL++S
Sbjct: 722  GRMKPEIQIHMQGSGIRELPSSIFQYKTHVTKLLLWNMKNLVALPSSICRLKSLVSLSVS 781

Query: 785  GCSNLANRKRKASEAELEELDIRGIARRRRRRDDNDN-----DNQLVGLNIFRKLFFWLC 844
            GCS L     ++   E+ +LD        R  D +D       + ++ LN    L F   
Sbjct: 782  GCSKL-----ESLPEEIGDLD------NLRVFDASDTLILRPPSSIIRLNKLIILMFRGF 841

Query: 845  KAPASCIFGIPSLAGLYSLTRLNLRDCNL--EVIPRGIECLVSLTELNLCGNNFSHLPTS 904
            K      F  P   GL+SL  LNL  CNL    +P  I  L SL +L+L  NNF HLP+S
Sbjct: 842  KDGVHFEFP-PVAEGLHSLEYLNLSYCNLIDGGLPEEIGSLSSLKKLDLSRNNFEHLPSS 901

Query: 905  ISQLHNLKRLNINQCKKLVHFPELPPRILRLLSKDC-VSLKSIPD-ISKVEHLYFLKELN 964
            I+QL  L+ L++  C++L   PELPP  L  L  DC ++LK I   ++K + L+ +K  +
Sbjct: 902  IAQLGALQSLDLKDCQRLTQLPELPPE-LNELHVDCHMALKFIHYLVTKRKKLHRVKLDD 961

Query: 965  LMN-------CYQLANNKELHRLIISWMEKMLFRKGAFNIMIPGSEIPDWFTTK-MGSSI 1024
              N        Y +  N    R  IS  + +      F       +IP WF  +   SS+
Sbjct: 962  AHNDTMYNLFAYTMFQNISSMRHDISASDSLSLT--VFTGQPYPEKIPSWFHHQGWDSSV 1021

Query: 1025 SVKWDPN--SPNAHLIRFALCVLCGPIDDQDDMTDVSGSIIASVTGK------SHEDSNL 1048
            SV    N   P+  L  FA+C     ID    +  V    ++ +T K        E SN 
Sbjct: 1022 SVNLPENWYIPDKFL-GFAVCYSRSLIDTTAHLIPVCDDKMSRMTQKLALSECDTESSNY 1057

BLAST of Lcy12g002500 vs. ExPASy Swiss-Prot
Match: Q9SZ66 (Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=1 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 1.6e-155
Identity = 401/1198 (33.47%), Postives = 617/1198 (51.50%), Query Frame = 0

Query: 8    SSSGKWKYDVFLSFRGEDTRGGFTDHLYKALVRKGIFTFRDEDEIKEGKGISSDLLAAIE 67
            SS    ++DVFLSFRG DTR  FT HL KAL  +GI +F D D ++ G  +++ L   IE
Sbjct: 4    SSPSSAEFDVFLSFRGFDTRNNFTGHLQKALRLRGIDSFID-DRLRRGDNLTA-LFDRIE 63

Query: 68   ASRFALVVVSQNYASSRWCLEELVKIFDCQGRLRMSVLPVFYKVDPSDVRNQRGSFGEAF 127
             S+ A++V S NYA+S WCL ELVKI +C+   +  V+P+FYKVD SDV  QR SF   F
Sbjct: 64   KSKIAIIVFSTNYANSAWCLRELVKILECRNSNQQLVVPIFYKVDKSDVEKQRNSFAVPF 123

Query: 128  VKHELRF-GEDDSKIRKWRQALTKLANLKAWLSQPWS-HESKIIEEITEAIWKRIKPTLT 187
               EL F G    +I  W+ AL   +N+  ++ +  S  E+K+++EI    +K++   L 
Sbjct: 124  KLPELTFPGVTPEEISSWKAALASASNILGYVVKEISTSEAKLVDEIAVDTFKKLN-DLA 183

Query: 188  VTQEDQLVGINSKLNKLFSLLNPNLVDDVVCVGIHGMGGIGKTTIARVCYERIRDEFEAH 247
             +  + LVGI S+L  L  LL+   +D V  +GI GM GIGKTT+A   Y R+R +F+  
Sbjct: 184  PSGNEGLVGIESRLKNLEKLLSWEDLDTVHIIGIVGMVGIGKTTLADCLYGRMRGQFDGS 243

Query: 248  CFLSDVREKFETSGLPHLQSKLLSRMFSIKNNDIWDVEEGIAMINRAIFQKKTLLVLDDV 307
            CFL+++RE    SGL  L  KL S + + ++ +I           R +  K+ L+VLDDV
Sbjct: 244  CFLTNIRENSGRSGLESLLQKLFSTVLNDRDLEIGAPGNAHERFERRLKSKRLLIVLDDV 303

Query: 308  DSSDQITGLIPNKGSFGSGSRIIITTRNADLLSNEFEVKRIFEVEELQYEEALQLLNLSA 367
            +   QI  L+ +   +  GSRIIITTR++ L+  E    R + + +L   EAL+L +L+A
Sbjct: 304  NDEKQIRYLMGHCKWYQGGSRIIITTRDSKLI--ETIKGRKYVLPKLNDREALKLFSLNA 363

Query: 368  FTKTCPKECFLEHSKSIVKVVGGHPLALKLLGSSLRNKDLSVWNDVIEELQGGGNIHDKI 427
            F+ + P + F   +  ++    GHPLALK+LGS L  +D   W   ++ L+     H  I
Sbjct: 364  FSNSFPLKEFEGLTNMVLDYAKGHPLALKVLGSDLCERDDLYWEAKLDRLK--SRSHGDI 423

Query: 428  FKCLKVSYDGLDEWEKEIFLDVACFFKGKRRELVEEMLNGCGFYAKRRVELLIQKSLLTL 487
            ++ L+ SY+ L   +K +FLD+ACFF+ +  + V  +LN  G      V+ L+ K L+TL
Sbjct: 424  YEVLETSYEELTTEQKNVFLDIACFFRSENVDYVTSLLNSHGVDVSGVVKDLVDKCLITL 483

Query: 488  SYDNRLHMHDLLQEMGRKI---VRHKPIQD---------------RLWCHKDIKSVVREA 547
            S DNR+ MHD+LQ M ++I   V    I+D               RLW  +DI  ++ E 
Sbjct: 484  S-DNRIEMHDMLQTMAKEISLKVETIGIRDCRWLSRHGNQCQWHIRLWDSEDICDLLTEG 543

Query: 548  L----VQSIFFKSTRI-VVEFPIQ-FSKMHQLRLLNFKN------------VRLKSQLEY 607
            L    ++ IF  ++++  +    + F  M+ L+ L   +            + L+  L +
Sbjct: 544  LGTDKIRGIFLDTSKLRAMRLSAKAFQGMYNLKYLKIYDSHCSRGCEAEFKLHLRRGLSF 603

Query: 608  CIPTELRYLKWKGYPMEFLPLSSEEYKLIGLHMCHSNLKQFWQGEKHLEELKYIKLNHSQ 667
             +P EL YL W GYP++ +PL  +   L+ L + HS L++ W  EK +  LK++ L+HS 
Sbjct: 604  -LPNELTYLHWHGYPLQSIPLDFDPKNLVDLKLPHSQLEEIWDDEKDVGMLKWVDLSHSI 663

Query: 668  KLSKTPDFTKIPNLERLELEGCTSLVNIHPSIFLAEKLIFLSLKDCINLTNLPRDINIKV 727
             L +        NLERL LEGCTSL  +  +I   EKLI+L+L+DC +L +LP+ I  + 
Sbjct: 664  NLRQCLGLANAHNLERLNLEGCTSLKKLPSTINCLEKLIYLNLRDCTSLRSLPKGIKTQS 723

Query: 728  LQVLILSGCSKVNNIPEFSGNTNRLLQLHLDGTSISNLPPSIATLTHLKVLSLKNCSKLI 787
            LQ LILSGCS +   P  S N   LL   LDGT I +LP SI T   L +L+LKNC KL 
Sbjct: 724  LQTLILSGCSSLKKFPLISENVEVLL---LDGTVIKSLPESIQTFRRLALLNLKNCKKLK 783

Query: 788  NIPNAIDKMKALQSLNLSGCSNLANRKRKASEAELEELDIRGIARRRRRRDDNDNDNQLV 847
            ++ + + K+K LQ L LSGCS L        + ++E L+I          DD        
Sbjct: 784  HLSSDLYKLKCLQELILSGCSQL--EVFPEIKEDMESLEI-------LLMDDTSITEMPK 843

Query: 848  GLNIFRKLFFWLCKAPAS---CIFGIPSLAGLYSLTRLNLRDCNLEVIPRGIECLVSLTE 907
             +++     F LC   +     +F +P   G   LT L L  C+L  +P  I  L SL  
Sbjct: 844  MMHLSNIKTFSLCGTSSHVSVSMFFMPPTLGCSRLTDLYLSRCSLYKLPDNIGGLSSLQS 903

Query: 908  LNLCGNNFSHLPTSISQLHNLKRLNINQCKKLVHFPELPPRILRLLSKDCVSLKSIPD-- 967
            L L GNN  +LP S +QL+NLK  ++  CK L   P LP  +  L + +C SL+++ +  
Sbjct: 904  LCLSGNNIENLPESFNQLNNLKWFDLKFCKMLKSLPVLPQNLQYLDAHECESLETLANPL 963

Query: 968  ----ISKVEHLYFLKELNLMNCYQLANNKEL---------HRLIISWMEKMLFR----KG 1027
                + +  H  F+      NCY+L  + +           +L+ +   K  +R    + 
Sbjct: 964  TPLTVGERIHSMFI----FSNCYKLNQDAQASLVGHARIKSQLMANASAKRYYRGFVPEP 1023

Query: 1028 AFNIMIPGSEIPDWFT-TKMGSSISVKWDPNSPNAHLIRFALCVLCGPIDDQDDMTDVSG 1087
               I  P +EIP WF   ++G S+ +   P+  + + +  AL V+    D +D     S 
Sbjct: 1024 LVGICYPATEIPSWFCHQRLGRSLEIPLPPHWCDINFVGLALSVVVSFKDYEDSAKRFSV 1083

Query: 1088 SIIASVTGKSHEDSNLKNGDLLVNGFT------ISGMRKL--DHIWL-----FVLPRTRS 1130
                +   K   DS+    D  + G+           RKL  DH+++     F++   ++
Sbjct: 1084 KCCGNFENK---DSSFTRFDFTLAGWNEPCGSLSHESRKLTSDHVFMGYNSCFLV---KN 1143

BLAST of Lcy12g002500 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 3.6e-155
Identity = 362/979 (36.98%), Postives = 541/979 (55.26%), Query Frame = 0

Query: 5   SSCSSSGKWKYDVFLSFRGEDTRGGFTDHLYKALVRKGIFTFRDEDEIKEGKGISSDLLA 64
           +S S  G+  YDVFLSFRGEDTR  F  HL+ AL+ KGI TF D+ E+K GK ISS+L+ 
Sbjct: 3   TSSSHHGR-SYDVFLSFRGEDTRKTFVGHLFNALIEKGIHTFMDDKELKRGKSISSELMK 62

Query: 65  AIEASRFALVVVSQNYASSRWCLEELVKIFDCQGRLRMSVLPVFYKVDPSDVRNQRGSFG 124
           AI  SRFA+VV S+NYASS WCLEELVKI +   +  + V+PVFY VDPS VR Q G + 
Sbjct: 63  AIGESRFAVVVFSKNYASSTWCLEELVKILEIHEKFELIVVPVFYDVDPSTVRKQNGEYA 122

Query: 125 EAFVKHELRFGEDDSKIRKWRQALTKLANLKAW-LSQPWS-HESKIIEEITEAIWKRIKP 184
             F K E    +D  K+ +WR+ALTK+AN+    L   ++  ESK I++I + I+ +   
Sbjct: 123 VCFTKFEANLVDDRDKVLRWREALTKVANISGHDLRNTYNGDESKCIQQILKDIFDKFCF 182

Query: 185 TLTVTQEDQLVGINSKLNKLFSLLNPNLVDDVVCVGIHGMGGIGKTTIARVCYERIRDEF 244
           ++++T  D LVGI S++ KL SLL  +L   V  VGI GMGG+GKTT AR  + R    F
Sbjct: 183 SISITNRD-LVGIESQIKKLSSLLRMDL-KGVRLVGIWGMGGVGKTTAARALFNRYYQNF 242

Query: 245 EAHCFLSDVREKFETSGLPHLQSKLLSRMFSIKNNDIWDVEEGIAMINRAIFQKKTLLVL 304
           E+ CFL DV+E  +   L +LQ  LLS++  ++  D  D EE   ++ R +  KK L+VL
Sbjct: 243 ESACFLEDVKEYLQHHTLLYLQKTLLSKLLKVEFVDCTDTEEMCVILKRRLCSKKVLVVL 302

Query: 305 DDVDSSDQITGLIPNKGSFGSGSRIIITTRNADLLSNEFEVKRIFEVEELQYEEALQLLN 364
           DDV+ +DQ+  L+  +  FGSGSRI+ITTR+  LL N  +V   +E++ L+ +EA++L N
Sbjct: 303 DDVNHNDQLDKLVGAEDWFGSGSRIVITTRDMKLLKNH-DVHETYEIKVLEKDEAIELFN 362

Query: 365 LSAFTKTCPKECFLEHSKSIVKVVGGHPLALKLLGSSLRNKDLSVWNDVIEELQGGGNIH 424
           L AF ++ P++ F E    +V   GG PLALK+LGS L  +DL VW   I+ L+   N  
Sbjct: 363 LHAFKRSSPEKEFKELLNLVVDYTGGLPLALKVLGSLLYKEDLDVWISTIDRLK--DNPE 422

Query: 425 DKIFKCLKVSYDGLDEWEKEIFLDVACFFKGKRRELVEEMLNGCGFYAKRRVELLIQKSL 484
            +I   LK+S+DGL ++EK IFLD+ACFF+G  +  +  + +  GF+    V+ L++KSL
Sbjct: 423 GEIMATLKISFDGLRDYEKSIFLDIACFFRGYNQRDMTALFHASGFHPVLGVKTLVEKSL 482

Query: 485 LTLSYDNRLHMHDLLQEMGRKIVRHKPIQDRLWCHKDIKSV----VREALVQSIFFKSTR 544
           + +  ++++ MHDL+QEMGR+I   +    R++  +D+K      +R+  ++ +      
Sbjct: 483 IFI-LEDKIQMHDLMQEMGRQIAVQESPMRRIYRPEDVKDACIGDMRKEAIEGLLLTEPE 542

Query: 545 IVVEFPIQF-------SKMHQLRLL--NFKNVRLKSQLEYCIPTELRYLKWKGYPMEFLP 604
              E  +++        K  +LR+L   + N      + Y +P  L +L+W+ Y     P
Sbjct: 543 QFEEGELEYMYSAEALKKTRRLRILVKEYYNRGFDEPVAY-LPNSLLWLEWRNYSSNSFP 602

Query: 605 LSSEEYKLIGLHMCHSNLKQFWQGEKHLEELKYIKLNHSQKLSKTPDFTKIPNLERLELE 664
            + E  KL+ L M  S++ + W G K L  L  + L++  KL +TPDF  I NLERL L 
Sbjct: 603 SNFEPSKLVYLTMKGSSIIELWNGAKRLAFLTTLDLSYCHKLIQTPDFRMITNLERLILS 662

Query: 665 GCTSLVNIHPSIFLAEKLIFLSLKDCINLTNLPRDINIKVLQVLILSGCSKVNNIPEFSG 724
            C +LV +HPS+   + LI L++  CI+L  LP  I  + L+VL L+ C  +   PE   
Sbjct: 663 SCDALVEVHPSVGFLKNLILLNMDHCISLERLPAIIQSECLEVLDLNYCFNLKMFPEVER 722

Query: 725 NTNRLLQLHLDGTSISNLPPSIATLTHLKVLSLKNCSKLINIPNAIDKMKALQSLNLSGC 784
           N   L +L L  T I  LP SI  L+ L+ L + +C++L+++P++I + +   +L +S C
Sbjct: 723 NMTHLKKLDLTSTGIRELPASIEHLSSLENLQMHSCNQLVSLPSSIWRFR---NLKISEC 782

Query: 785 SNLANRKRKASEAELEELDIRGIARRRRRRDDNDNDNQLVGLNIFRKLFFWLCKAPASCI 844
             L +       +      I  +   +       N   L  L I        CK  +S  
Sbjct: 783 EKLGSLPEIHGNSNCTRELILKLVSIKELPTSIGNLTSLNFLEICN------CKTISSL- 842

Query: 845 FGIPSLAGLYSLTRLNLRDC----NLEVIP--------RGIECLVSL------------- 904
               S+ GL SLT L L DC    NL  IP         G++ L++L             
Sbjct: 843 --SSSIWGLTSLTTLKLLDCRKLKNLPGIPNAINHLSGHGLQLLLTLEQPTIYERLDLLR 902

Query: 905 -TELNLCGNNFSHLPTSISQLHNLKRLNINQCKKLVHFPELPPRI--LRLLSKDCVSLKS 937
             +++ C +  S LP +I  L  L+ L I+ C +L + PE    +  L  L  D   +  
Sbjct: 903 IIDMSWC-SCISSLPHNIWMLKFLRILCISYCSRLEYLPENLGHLEHLEELLADGTGILR 960

BLAST of Lcy12g002500 vs. ExPASy TrEMBL
Match: A0A1S3CBN9 (TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498654 PE=4 SV=1)

HSP 1 Score: 1735.3 bits (4493), Expect = 0.0e+00
Identity = 882/1141 (77.30%), Postives = 994/1141 (87.12%), Query Frame = 0

Query: 4    SSSCSSSGKWKYDVFLSFRGEDTRGGFTDHLYKALVRKGIFTFRDEDEIKEGKGISSDLL 63
            SSS SS GKWK+DVFLSFRGEDTRGGFTDHLYKAL +KGI TFRDE+EI+EG+ ISS+LL
Sbjct: 7    SSSSSSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTQKGISTFRDENEIQEGEDISSNLL 66

Query: 64   AAIEASRFALVVVSQNYASSRWCLEELVKIFDCQGRLRMSVLPVFYKVDPSDVRNQRGSF 123
             +IEASRFA+VVVS+NYASSRWCLEELVKIF+C+ +L M VLP+FYKVDPS VRNQRG F
Sbjct: 67   DSIEASRFAIVVVSENYASSRWCLEELVKIFECEEKLGMDVLPIFYKVDPSHVRNQRGRF 126

Query: 124  GEAFVKHELRFGEDDSKIRKWRQALTKLANLKAWLSQPWSHESKIIEEITEAIWKRIKPT 183
             EAFVKHE+RFG DD K++KWR+ LTKLANLKAWLSQ WSHES IIEEIT  IWKR+K  
Sbjct: 127  EEAFVKHEMRFGRDDIKVQKWRRLLTKLANLKAWLSQSWSHESNIIEEITTTIWKRLKHN 186

Query: 184  LTVTQEDQLVGINSKLNKLFSLLNPNL----VDDVVCVGIHGMGGIGKTTIARVCYERIR 243
            LTV +EDQLVGINSKLNKL SLL PN      DDV+ VGIHGMGGIGKTTIA+VCY+RIR
Sbjct: 187  LTVIKEDQLVGINSKLNKLSSLLIPNSDEDEDDDVIFVGIHGMGGIGKTTIAKVCYQRIR 246

Query: 244  DEFEAHCFLSDVREK-FETSG-LPHLQSKLLSRMFSIKNNDIWDVEEGIAMINRAIFQKK 303
            DEFEAHCFLSDVRE  F TSG LP+LQ+KLLSRMFS KNN I DVEEGIAMIN+AIF+KK
Sbjct: 247  DEFEAHCFLSDVRENYFRTSGDLPYLQTKLLSRMFSFKNNHILDVEEGIAMINKAIFRKK 306

Query: 304  TLLVLDDVDSSDQITGLIPNKGSFGSGSRIIITTRNADLLSNEFEVKRIFEVEELQYEEA 363
            TLLVLDDVD SDQI GLIPNK SFG+GSRIIITTRNADLLSNEF VKRIFE++EL+YEEA
Sbjct: 307  TLLVLDDVDCSDQIMGLIPNKSSFGNGSRIIITTRNADLLSNEFGVKRIFEMDELKYEEA 366

Query: 364  LQLLNLSAFTKTCPKECFLEHSKSIVKVVGGHPLALKLLGSSLRNKDLSVWNDVIEELQG 423
            LQLL+LSAF KTCPKE +LEHSK IVKVVGGHPLALKLLGSSLRNK+LSVWN+VIEE++G
Sbjct: 367  LQLLSLSAFMKTCPKEGYLEHSKKIVKVVGGHPLALKLLGSSLRNKNLSVWNEVIEEVEG 426

Query: 424  GGNIHDKIFKCLKVSYDGLDEWEKEIFLDVACFFKGKRRELVEEMLNGCGFYAKRRVELL 483
            GGNIH+KIFKCLKVSYDGLDEWEKEIFLDVACFF GKRRE+VEE+LNGCGFYAK R+ELL
Sbjct: 427  GGNIHEKIFKCLKVSYDGLDEWEKEIFLDVACFFNGKRREVVEEILNGCGFYAKTRIELL 486

Query: 484  IQKSLLTLSYDNRLHMHDLLQEMGRKIVRHKPIQDRLWCHKDIKSVVREALVQSIFFK-S 543
            IQKSLLTLSYDN+LHMHDLLQEMGRKIVR K ++DRL CHKDIKSVV E LVQSIFFK S
Sbjct: 487  IQKSLLTLSYDNKLHMHDLLQEMGRKIVRDKHVRDRLMCHKDIKSVVTETLVQSIFFKSS 546

Query: 544  TRIVVEFPIQFSKMHQLRLLNFKNVRLKSQLEYCIPTELRYLKWKGYPMEFLPL-SSEEY 603
            ++ +VEFPI FS+MHQLRLLNF NVRLK++LEYCIP+ELRYLKWK YP+EFLP+ SSEE 
Sbjct: 547  SKNMVEFPILFSRMHQLRLLNFHNVRLKNKLEYCIPSELRYLKWKEYPLEFLPINSSEEC 606

Query: 604  KLIGLHMCHSNLKQFWQGEKHLEELKYIKLNHSQKLSKTPDFTKIPNLERLELEGCTSLV 663
            KLI LHMCHSNLKQFWQ EK+L  LKYIKLN SQKLSKTP+F  IPNL+RLELE CTSLV
Sbjct: 607  KLIELHMCHSNLKQFWQQEKNLVGLKYIKLNSSQKLSKTPNFANIPNLKRLELEDCTSLV 666

Query: 664  NIHPSIFLAEKLIFLSLKDCINLTNLPRDINIKVLQVLILSGCSKVNNIPEFSGNTNRLL 723
            NIHPSIF AEKLIFL+LKDCINLTNLP  INIKVL+VLILSGCSKV  +PEFSGNTNRLL
Sbjct: 667  NIHPSIFTAEKLIFLNLKDCINLTNLPSRINIKVLEVLILSGCSKVKKVPEFSGNTNRLL 726

Query: 724  QLHLDGTSISNLPPSIATLTHLKVLSLKNCSKLINIPNAIDKMKALQSLNLSGCSNLANR 783
            QLHLDGTSISNLP SIA+L+HL +LSL NC KLINI NA++ M +LQSL++SGC  L +R
Sbjct: 727  QLHLDGTSISNLPSSIASLSHLTILSLANCKKLINISNAME-MTSLQSLDVSGCLKLGSR 786

Query: 784  KRKASEAELEELDIRGIARRRRRRDDNDNDNQLVGLNIFRKLFFWLCKAPASCIFGIPSL 843
            KRKA + EL ELD+R   RRRR  D N         NIF+K+F WLCK PAS IFGIPSL
Sbjct: 787  KRKADDGELGELDVRETTRRRRNDDSN---------NIFKKIFLWLCKTPASGIFGIPSL 846

Query: 844  AGLYSLTRLNLRDCNLEVIPRGIECLVSLTELNLCGNNFSHLPTSISQLHNLKRLNINQC 903
            AGLYSLT+LNLRDCNLE IP+GIECLVSL EL+L GN+FSHLPTSIS+LHNLK+L INQC
Sbjct: 847  AGLYSLTKLNLRDCNLEEIPQGIECLVSLVELDLSGNSFSHLPTSISRLHNLKKLRINQC 906

Query: 904  KKLVHFPELPPRILRLLSKDCVSLKSIPDISKVEHLYFLKELNLMNCYQLANNKELHRLI 963
             KLV FP+LPPRIL L+SKDC+SLK   DISKV++LY +KE+NL+NCYQLANNK  HRLI
Sbjct: 907  IKLVQFPKLPPRILFLMSKDCISLKDFVDISKVDNLYIMKEVNLLNCYQLANNKGFHRLI 966

Query: 964  ISWMEKMLFRKGAFNIMIPGSEIPDWFTT-KMGSSISVKWDPNSPNAHLIRFALCVLCGP 1023
            ISWM+KMLFRKG FNIMIPGSEIPDWFTT KMGSS+ ++WDP+ PN ++IRFALCV+ G 
Sbjct: 967  ISWMQKMLFRKGTFNIMIPGSEIPDWFTTRKMGSSVCIEWDPDGPNTNMIRFALCVVFGL 1026

Query: 1024 IDDQDDMTDVSGSIIASVTGKSHEDSNLKN-GDLLVNGFTISGMRKLDHIWLFVLPRTRS 1083
             +  D +   S +IIASVTGK   DSNLKN GDLL+ GF ++GM+KLDHIW+FVLPRT +
Sbjct: 1027 SEKIDVVNVPSFAIIASVTGKDRNDSNLKNGGDLLIGGFPVAGMKKLDHIWMFVLPRTGT 1086

Query: 1084 LTRKINKCKEIEFRFLFQ-ANYGQSVTSNIELKKCGVGLINMEKEKEAMKCYASRIILKN 1134
            L RKI+  KEI+FRFL Q ANY QS+T N+++K+CGVGLIN+E+EKEAMK YAS IIL+N
Sbjct: 1087 LVRKISNYKEIKFRFLLQAANYRQSITPNVKVKECGVGLINLEEEKEAMKRYASHIILRN 1137

BLAST of Lcy12g002500 vs. ExPASy TrEMBL
Match: A0A6J1CKI7 (TMV resistance protein N-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012132 PE=4 SV=1)

HSP 1 Score: 1714.1 bits (4438), Expect = 0.0e+00
Identity = 872/1146 (76.09%), Postives = 985/1146 (85.95%), Query Frame = 0

Query: 1    MVVSSSCSSSGKWKYDVFLSFRGEDTRGGFTDHLYKALVRKG-IFTFRDEDEIKEGKGIS 60
            M VSSS SS+GK+KYDVFLSFRGEDTRGGFTDHLY AL R G IFTFRDE+EI++G  IS
Sbjct: 6    MAVSSSSSSTGKYKYDVFLSFRGEDTRGGFTDHLYAALKRNGIIFTFRDENEIQQGADIS 65

Query: 61   SDLLAAIEASRFALVVVSQNYASSRWCLEELVKIFDCQGRLRMSVLPVFYKVDPSDVRNQ 120
            SDL AAIEASR A+VVVS+NYASSRWCL EL++IF+C+ RL M+VLP+FYKVDPSDVRNQ
Sbjct: 66   SDLSAAIEASRIAVVVVSENYASSRWCLGELLEIFECRKRLGMTVLPIFYKVDPSDVRNQ 125

Query: 121  RGSFGEAFVKHELRFGEDDSKIRKWRQALTKLANLKAWLSQPWSHESKIIEEITEAIWKR 180
            RGSF EAF KH  RFGE+D ++ KWRQALT+LANLKAWLSQ WSHES+IIEEI+ +IWKR
Sbjct: 126  RGSFAEAFAKHGERFGEEDFRLTKWRQALTQLANLKAWLSQHWSHESEIIEEISASIWKR 185

Query: 181  IKPTLTVTQEDQLVGINSKLNKLFSLLNPNLVDD----VVCVGIHGMGGIGKTTIARVCY 240
            IKPTLT+T+EDQL+GINSKL+KL SLLN N   D    V  VGIHGMGGIGKTTIARV Y
Sbjct: 186  IKPTLTLTREDQLLGINSKLDKLSSLLNRNSNSDDDDGVFWVGIHGMGGIGKTTIARVFY 245

Query: 241  ERIRDEFEAHCFLSDVREKFETSGLPHLQSKLLSRM-FSIKNNDIWDVEEGIAMINRAIF 300
            E+IRDEF+AHCFLSDVREKFETSGL +L SKLLSR+ FS+KNNDIWD EEGIAMI+RAIF
Sbjct: 246  EQIRDEFDAHCFLSDVREKFETSGLTYLHSKLLSRLTFSMKNNDIWDAEEGIAMISRAIF 305

Query: 301  QKKTLLVLDDVDSSDQITGLIPNKGSFGSGSRIIITTRNADLLSNEFEVKRIFEVEELQY 360
            QKKTLLVLDDVD SDQI GLIP+   FG GSRIIITTRNADLLSNE  VKRI E+EEL+Y
Sbjct: 306  QKKTLLVLDDVDHSDQIKGLIPSNDFFGYGSRIIITTRNADLLSNELGVKRILEIEELKY 365

Query: 361  EEALQLLNLSAFTKTCPKECFLEHSKSIVKVVGGHPLALKLLGSSLRNKDLSVWNDVIEE 420
            EEALQ L+LS F KTCP+E F EH KSI+K+VGGHPL LKLLG+ LRNKDL VW+ VIEE
Sbjct: 366  EEALQHLSLSVFMKTCPEEGFXEHCKSIIKLVGGHPLGLKLLGTCLRNKDLGVWDCVIEE 425

Query: 421  LQGGGNIHDKIFKCLKVSYDGLDEWEKEIFLDVACFFKGKRRELVEEMLNGCGFYAKRRV 480
            LQ GGNI ++IFKCLKVSYDGLDE EK+IFLDVACFFKGKRR+ VEE+L+GCGFYA+RR+
Sbjct: 426  LQEGGNIDNQIFKCLKVSYDGLDEREKDIFLDVACFFKGKRRDFVEEILDGCGFYARRRI 485

Query: 481  ELLIQKSLLTLSYDNRLHMHDLLQEMGRKIVRHKPI-QDRLWCHKDIKSVVREALVQSIF 540
            ELLIQKSL+TLSYDN+L MHDLLQEMGRKIVR KP  QDRLWCHKDIK VV EALVQSI+
Sbjct: 486  ELLIQKSLITLSYDNKLQMHDLLQEMGRKIVREKPTQQDRLWCHKDIKCVVTEALVQSIY 545

Query: 541  FKSTRIVVEFPIQFSKMHQLRLLNFKNVRLKSQLEYCIPTELRYLKWKGYPMEFLPLSSE 600
            FKS R  VE PI FS+M QLRLLNF NVRL++ LEYCIPTELRYLKWK YP+EFL LSSE
Sbjct: 546  FKSPRNTVEVPILFSRMQQLRLLNFHNVRLQNHLEYCIPTELRYLKWKRYPLEFLRLSSE 605

Query: 601  EYKLIGLHMCHSNLKQFWQGEKHLEELKYIKLNHSQKLSKTPDFTKIPNLERLELEGCTS 660
            EYKLI LH CHSNLKQFW  EKHLE+LKYIKLNHSQKLSKTPDF KIPNL+RLELEGCTS
Sbjct: 606  EYKLITLHXCHSNLKQFWPVEKHLEKLKYIKLNHSQKLSKTPDFAKIPNLKRLELEGCTS 665

Query: 661  LVNIHPSIFLAEKLIFLSLKDCINLTNLPRDINIKVLQVLILSGCSKVNNIPEFSGNTNR 720
            LVNIHPSIF AEKLIFLSL+DC NLTNLP  INIKVL+VLILSGCSK+N +PEFSGNTNR
Sbjct: 666  LVNIHPSIFSAEKLIFLSLRDCTNLTNLPSHINIKVLEVLILSGCSKLNKVPEFSGNTNR 725

Query: 721  LLQLHLDGTSISNLPPSIATLTHLKVLSLKNCSKLINIPNAIDKMKALQSLNLSGCSNLA 780
            LLQLH D TSI+NLP S+A+L  L VLSLKNC KL+NI + IDKM +L+SLNLSGCS L 
Sbjct: 726  LLQLHFDATSIANLPSSVASLNRLTVLSLKNCKKLVNICSVIDKMTSLESLNLSGCSKLG 785

Query: 781  NRKRKASE--AELEELDIRGIARRRRRRDDNDNDNQLVGLNIFRKLFFWLCKAPASCIFG 840
            NRKRK  +    L ELD+RG  RRR       +D   VG+NIF++LF WLCKAP S IFG
Sbjct: 786  NRKRKIDDNVGCLAELDVRGAPRRR-------DDEGKVGVNIFKQLFLWLCKAPESGIFG 845

Query: 841  IPSLAGLYSLTRLNLRDCNLEVIPRGIECLVSLTELNLCGNNFSHLPTSISQLHNLKRLN 900
            +PSLAGLYSLT+LNL+DCNLE IP+GIECLVSLTEL LCGNNFSHLPTSIS+LHNL+RLN
Sbjct: 846  VPSLAGLYSLTKLNLKDCNLETIPQGIECLVSLTELRLCGNNFSHLPTSISRLHNLRRLN 905

Query: 901  INQCKKLVHFPELPPRILRLLSKDCVSLKSIPDISKVEHLYFLKELNLMNCYQLANNKEL 960
            IN CKKL+ FPELPPRI  L+SK C+SLK+IP+ISKV+H YF++E+NL+NCYQLA+N  L
Sbjct: 906  INGCKKLLCFPELPPRIRWLMSKGCISLKNIPNISKVDHSYFMREVNLLNCYQLADNIGL 965

Query: 961  HRLIISWMEKMLFRKGAFNIMIPGSEIPDWFT-TKMGSSISVKWDPNSPNAHLIRFALCV 1020
            HRLI  WMEKMLFRKG FNIMIPGS+IPDWFT T+MGSSI V+WDP++ NA+LIRFALCV
Sbjct: 966  HRLITYWMEKMLFRKGTFNIMIPGSQIPDWFTMTEMGSSICVEWDPDAANANLIRFALCV 1025

Query: 1021 LCGPIDDQDDMTDVSGSIIASVTGKSHEDSNLKNGDLLVNGFTISGMRKLDHIWLFVLPR 1080
            +CGP  +Q D+ D   +IIASVTG+  +D  L NGDL+VN F +SGMRKLDHIWLFVLPR
Sbjct: 1026 VCGP-TNQGDIIDTPFAIIASVTGRGADDPCLNNGDLIVNAFNVSGMRKLDHIWLFVLPR 1085

Query: 1081 TRSLTRKINKCKEIEFRFLFQANYGQSVTS-NIELKKCGVGLINMEKEKEAMKCYASRII 1136
            T+SL RKI+ CKEI+F+FL Q NY +S +S +I LKKCGV LINME+EKEAMK YAS II
Sbjct: 1086 TKSLARKISTCKEIKFQFLVQVNYSRSASSPSIALKKCGVHLINMEEEKEAMKRYASYII 1143

BLAST of Lcy12g002500 vs. ExPASy TrEMBL
Match: A0A6J1I8R5 (TMV resistance protein N-like OS=Cucurbita maxima OX=3661 GN=LOC111472025 PE=4 SV=1)

HSP 1 Score: 1702.6 bits (4408), Expect = 0.0e+00
Identity = 850/1132 (75.09%), Postives = 969/1132 (85.60%), Query Frame = 0

Query: 5    SSCSSSGKWKYDVFLSFRGEDTRGGFTDHLYKALVRKGIFTFRDEDEIKEGKGISSDLLA 64
            SS SS+GKWK++VFLSFRGEDTR GFTD LY AL+ KGI TFRDEDEI+EG  IS+DL A
Sbjct: 4    SSGSSTGKWKFNVFLSFRGEDTRFGFTDRLYNALIHKGISTFRDEDEIEEGADISTDLSA 63

Query: 65   AIEASRFALVVVSQNYASSRWCLEELVKIFDCQGRLRMSVLPVFYKVDPSDVRNQRGSFG 124
            AIEASR ALVVVS+NYASSRWCLEEL KIF+C  RL M+VLP+FYKVDPS VR Q G+F 
Sbjct: 64   AIEASRIALVVVSENYASSRWCLEELSKIFECHHRLGMTVLPIFYKVDPSHVRKQTGTFA 123

Query: 125  EAFVKHELRFGEDDSKIRKWRQALTKLANLKAWLSQPWSHESKIIEEITEAIWKRIKPTL 184
            EAF KHE+RFGE +  I+KWRQ LTKLANLKAWL +PW+HESK+IEEIT+ +WKRIKPTL
Sbjct: 124  EAFAKHEIRFGEHNPNIQKWRQLLTKLANLKAWLLEPWTHESKVIEEITKVLWKRIKPTL 183

Query: 185  TVTQEDQLVGINSKLNKLFSLLNPNLVDDVVCVGIHGMGGIGKTTIARVCYERIRDEFEA 244
             VTQE QLVGINSKL KL SLLNPN  +DV+ +GIHGMGGIGKTT+ARVCYERIRD+FEA
Sbjct: 184  RVTQEHQLVGINSKLTKLSSLLNPNSDEDVIWIGIHGMGGIGKTTLARVCYERIRDKFEA 243

Query: 245  HCFLSDVREKFETSGLPHLQSKLLSRMFSIKNNDIWDVEEGIAMINRAIFQKKTLLVLDD 304
            HCF+S+V+EKFETSGLP+LQS+LLSRMFSI+N DI DVEEGIAMIN+A+F+KK LLVLDD
Sbjct: 244  HCFVSNVQEKFETSGLPYLQSQLLSRMFSIENKDIGDVEEGIAMINQAVFRKKILLVLDD 303

Query: 305  VDSSDQITGLIPNKGSFGSGSRIIITTRNADLLSNEFEVKRIFEVEELQYEEALQLLNLS 364
            V+ SDQI GLIPNK SFG+GSRIIIT RNADLLSNE EVKR+F++ EL  EEALQLLNLS
Sbjct: 304  VNCSDQIMGLIPNKDSFGNGSRIIITARNADLLSNELEVKRMFKMVELTNEEALQLLNLS 363

Query: 365  AFTKTCPKECFLEHSKSIVKVVGGHPLALKLLGSSLRNKDLSVWNDVIEELQGGGNIHDK 424
            A     PK+  LEHSK+IVK+VGGHPLALKLLGSSLRNKDL VW  VIEEL+GGGNIH+K
Sbjct: 364  A----SPKQDCLEHSKNIVKIVGGHPLALKLLGSSLRNKDLKVWKYVIEELKGGGNIHEK 423

Query: 425  IFKCLKVSYDGLDEWEKEIFLDVACFFKGKRRELVEEMLNGCGFYAKRRVELLIQKSLLT 484
            +FKCLKVSYDGLDEWEKEIFLD+ACFFKGKR+ELVEE+L+ CGF+AK RVELLIQKSLLT
Sbjct: 424  VFKCLKVSYDGLDEWEKEIFLDIACFFKGKRKELVEEILHSCGFHAKIRVELLIQKSLLT 483

Query: 485  LSYDNRLHMHDLLQEMGRKIVRHKPIQDRLWCHKDIKSVVREALVQSIFFKSTRIVVEFP 544
            LSY N+L MHDLLQEMG+KIVRHKPIQDRLW  KDIKS+V EA V+SI FKSTR VVEFP
Sbjct: 484  LSYHNKLMMHDLLQEMGQKIVRHKPIQDRLWRQKDIKSMVGEASVESILFKSTRNVVEFP 543

Query: 545  IQFSKMHQLRLLNFKNVRLKSQLEYCIPTELRYLKWKGYPMEFLPLSSEEYKLIGLHMCH 604
            I FS+MHQLRLLNF NVRLK+ LEYCIP+ELRYLKWKGYP+E L L+SEEYKLI LHMCH
Sbjct: 544  ISFSRMHQLRLLNFHNVRLKNVLEYCIPSELRYLKWKGYPLESLVLNSEEYKLIKLHMCH 603

Query: 605  SNLKQFWQGEKHLEELKYIKLNHSQKLSKTPDFTKIPNLERLELEGCTSLVNIHPSIFLA 664
            S+LKQFW GEKHLEELKYIKLNHSQKLSKTP+F  IPNL RLEL GCTSLVNIHP+IF +
Sbjct: 604  SHLKQFWHGEKHLEELKYIKLNHSQKLSKTPNFETIPNLTRLELVGCTSLVNIHPTIFTS 663

Query: 665  EKLIFLSLKDCINLTNLPRDINIKVLQVLILSGCSKVNNIPEFSGNTNRLLQLHLDGTSI 724
            ++L FLSLKDCINLTN P  INIK L+VLILSGCSK+  IPEFSGNT++LL+LHLDGTSI
Sbjct: 664  KELTFLSLKDCINLTNFPPQINIKALEVLILSGCSKLKKIPEFSGNTDKLLELHLDGTSI 723

Query: 725  SNLPPSIATLTHLKVLSLKNCSKLINIPNAIDKMKALQSLNLSGCSNLANRKRKASEAEL 784
            S+LP SIA L HL VLSL NC  LINI NA+DKM +L+SLNLSGCS L NRKRK  + E 
Sbjct: 724  SSLPSSIAILDHLTVLSLTNCKNLINISNALDKMTSLKSLNLSGCSKLGNRKRKRVDVET 783

Query: 785  EELDIRGIARRRRRRDDNDNDNQLVGLNIFRKLFFWLCKAPASCIFGIPSLAGLYSLTRL 844
             ELD+R   R  RR DD D        NIFRK+F WLCKAP S IFGIPSLAGLYSLTRL
Sbjct: 784  VELDVR---RTARRTDDGD--------NIFRKIFLWLCKAPTSGIFGIPSLAGLYSLTRL 843

Query: 845  NLRDCNLEVIPRGIECLVSLTELNLCGNNFSHLPTSISQLHNLKRLNINQCKKLVHFPEL 904
            NL DC LE +P GIECLVSL  LNL  NNFS LPTSIS+LHNLKRLN+N+C+KL+HFP+L
Sbjct: 844  NLSDCKLEEVPEGIECLVSLVNLNLSRNNFSRLPTSISRLHNLKRLNVNECEKLLHFPKL 903

Query: 905  PPRILRLLSKDCVSLKSIPDISKVEHLYFLKELNLMNCYQLANNKELHRLIISWMEKMLF 964
            PPRILRL+SK C+SLK   DISKV+H YF+ E+NL+NC+Q  +NK+LH+LI SWM+KMLF
Sbjct: 904  PPRILRLMSKGCISLKDFLDISKVDHSYFMIEMNLLNCFQWVDNKDLHKLITSWMQKMLF 963

Query: 965  RKGAFNIMIPGSEIPDWFTT-KMGSSISVKWDPNSPNAHLIRFALCVLCGPIDDQDDMTD 1024
            RKGAFNI++PGSEIPDWFTT KMGSSI V+WD ++PNA+++RFALCV+CGP + Q D+ D
Sbjct: 964  RKGAFNILVPGSEIPDWFTTKKMGSSICVEWDHDAPNANMVRFALCVICGPSNKQ-DILD 1023

Query: 1025 VSGSIIASVTGKSHEDSNLKNGDLLVNGFTISGMRKLDHIWLFVLPRTRSLTRKINKCKE 1084
            V  +I ASVTGK+  D N  NGDL+V  F +SGM+KLDHIW+FVLPRT +LTRKI  CKE
Sbjct: 1024 VPVTIFASVTGKNRNDPNSNNGDLIVGAFNVSGMKKLDHIWMFVLPRTTTLTRKIRNCKE 1083

Query: 1085 IEFRFLFQANYGQSVTSNIELKKCGVGLINMEKEKEAMKCYASRIILKNKIR 1136
            IEFRFL Q NY Q+V  N+ELKKCGVGLINME+E EAMK YAS II+KN+++
Sbjct: 1084 IEFRFLLQFNYNQTVIPNVELKKCGVGLINMEEESEAMKRYASYIIVKNRMK 1119

BLAST of Lcy12g002500 vs. ExPASy TrEMBL
Match: A0A6J1EBI7 (TMV resistance protein N-like OS=Cucurbita moschata OX=3662 GN=LOC111432675 PE=4 SV=1)

HSP 1 Score: 1699.5 bits (4400), Expect = 0.0e+00
Identity = 849/1132 (75.00%), Postives = 965/1132 (85.25%), Query Frame = 0

Query: 5    SSCSSSGKWKYDVFLSFRGEDTRGGFTDHLYKALVRKGIFTFRDEDEIKEGKGISSDLLA 64
            SS SS+GKWK++VFLSFRGEDTR GFTD LY AL+ KGI TFRDEDEI+EG  IS+DL A
Sbjct: 4    SSGSSTGKWKFNVFLSFRGEDTRFGFTDRLYNALIHKGISTFRDEDEIEEGSDISTDLSA 63

Query: 65   AIEASRFALVVVSQNYASSRWCLEELVKIFDCQGRLRMSVLPVFYKVDPSDVRNQRGSFG 124
            AIEASR ALVVVS+NYASSRWCLEEL KIF+C  RL M+VLP+FYKVDPS VRNQ G+F 
Sbjct: 64   AIEASRIALVVVSENYASSRWCLEELSKIFECHHRLGMTVLPIFYKVDPSHVRNQTGTFA 123

Query: 125  EAFVKHELRFGEDDSKIRKWRQALTKLANLKAWLSQPWSHESKIIEEITEAIWKRIKPTL 184
            EAF KH+ RFGE +  I+KWR  LTKLANLKAWL +PW+HESK+IEEIT  +WKRIKPTL
Sbjct: 124  EAFAKHQHRFGEHNPNIQKWRHLLTKLANLKAWLLEPWTHESKVIEEITTVLWKRIKPTL 183

Query: 185  TVTQEDQLVGINSKLNKLFSLLNPNLVDDVVCVGIHGMGGIGKTTIARVCYERIRDEFEA 244
             VTQE QLVGINSKL KL SLLNPN  +DV+ VGIHGMGGIGKTT+ARVCYERIRD+FEA
Sbjct: 184  IVTQEHQLVGINSKLTKLSSLLNPNSDEDVIWVGIHGMGGIGKTTLARVCYERIRDKFEA 243

Query: 245  HCFLSDVREKFETSGLPHLQSKLLSRMFSIKNNDIWDVEEGIAMINRAIFQKKTLLVLDD 304
            HCF+S+V+EKFETSGLP+LQS+LLSRMFSI+N DI DVEEGIAMIN+A+F+KK LLVLDD
Sbjct: 244  HCFVSNVQEKFETSGLPYLQSQLLSRMFSIENKDIGDVEEGIAMINQAVFRKKILLVLDD 303

Query: 305  VDSSDQITGLIPNKGSFGSGSRIIITTRNADLLSNEFEVKRIFEVEELQYEEALQLLNLS 364
            V+ SDQI GLIPNK SFG+GSRIIITTRNADLLSNEFEVKR+F++ EL  EEALQLLNL 
Sbjct: 304  VNCSDQIMGLIPNKDSFGNGSRIIITTRNADLLSNEFEVKRMFKMVELTNEEALQLLNLR 363

Query: 365  AFTKTCPKECFLEHSKSIVKVVGGHPLALKLLGSSLRNKDLSVWNDVIEELQGGGNIHDK 424
            A    CPK+  LEHSK+IVK+VGGHPLALKLLGSSLRNKDL VW  V+EE++GGGNIH+K
Sbjct: 364  A----CPKQDCLEHSKNIVKIVGGHPLALKLLGSSLRNKDLRVWEYVMEEIKGGGNIHEK 423

Query: 425  IFKCLKVSYDGLDEWEKEIFLDVACFFKGKRRELVEEMLNGCGFYAKRRVELLIQKSLLT 484
            +FKCLKVSYDGLDEWEKEIFLDVACFFKGKR+ LVEE+LNGCGF+AK RVELL+QKSLLT
Sbjct: 424  VFKCLKVSYDGLDEWEKEIFLDVACFFKGKRKGLVEEILNGCGFHAKIRVELLLQKSLLT 483

Query: 485  LSYDNRLHMHDLLQEMGRKIVRHKPIQDRLWCHKDIKSVVREALVQSIFFKSTRIVVEFP 544
            LSY N+L +HDLLQEMGRKIVRHKPIQDRLW  KDIKS+V EA V+SI FKSTR V+EFP
Sbjct: 484  LSYHNKLMVHDLLQEMGRKIVRHKPIQDRLWRQKDIKSMVGEASVESILFKSTRNVMEFP 543

Query: 545  IQFSKMHQLRLLNFKNVRLKSQLEYCIPTELRYLKWKGYPMEFLPLSSEEYKLIGLHMCH 604
            I FS+MHQLRLLNF NVRLK++LEYCIP+ELRYLKWKGYP+E L L+SEE KLI LHMCH
Sbjct: 544  ISFSRMHQLRLLNFHNVRLKNELEYCIPSELRYLKWKGYPLENLVLNSEECKLIKLHMCH 603

Query: 605  SNLKQFWQGEKHLEELKYIKLNHSQKLSKTPDFTKIPNLERLELEGCTSLVNIHPSIFLA 664
            SNLKQFW GEKHLEELKYIKLNHS KLSKTP+F  IPNL RLELEGCTSLVNIHP+IF A
Sbjct: 604  SNLKQFWHGEKHLEELKYIKLNHSPKLSKTPNFETIPNLTRLELEGCTSLVNIHPTIFTA 663

Query: 665  EKLIFLSLKDCINLTNLPRDINIKVLQVLILSGCSKVNNIPEFSGNTNRLLQLHLDGTSI 724
            +KL FLSLKDCINLTN P  INIK L++LIL+GCSK+  IPEFSGNT+ LL+LHLDGTSI
Sbjct: 664  KKLTFLSLKDCINLTNFPPQINIKALEILILNGCSKLKKIPEFSGNTDILLELHLDGTSI 723

Query: 725  SNLPPSIATLTHLKVLSLKNCSKLINIPNAIDKMKALQSLNLSGCSNLANRKRKASEAEL 784
            S+LP SIA L HL VLSL NC  LINI NA+DK+ +L+SLNLSGCS L NRKRK  + E 
Sbjct: 724  SSLPSSIAILDHLTVLSLTNCKNLINISNALDKITSLKSLNLSGCSKLGNRKRKRGDVET 783

Query: 785  EELDIRGIARRRRRRDDNDNDNQLVGLNIFRKLFFWLCKAPASCIFGIPSLAGLYSLTRL 844
             E D+    RR  RR DN       G NIFRK+  WLCKAP S IFGIPSLAGLYSLTRL
Sbjct: 784  VEFDV----RRTARRTDN-------GDNIFRKIILWLCKAPTSGIFGIPSLAGLYSLTRL 843

Query: 845  NLRDCNLEVIPRGIECLVSLTELNLCGNNFSHLPTSISQLHNLKRLNINQCKKLVHFPEL 904
            NL DC LE +P GIECLVSL  LNL  NNFS LPTSIS+LHNLKRLN+N+C+KL+HFPEL
Sbjct: 844  NLSDCKLEEVPEGIECLVSLVNLNLSRNNFSRLPTSISRLHNLKRLNVNECEKLLHFPEL 903

Query: 905  PPRILRLLSKDCVSLKSIPDISKVEHLYFLKELNLMNCYQLANNKELHRLIISWMEKMLF 964
            PPRILRL+SK C+SLK   DISKV+H YF+ E+NLMNC+Q  +NKEL +LI SWM+KMLF
Sbjct: 904  PPRILRLMSKGCISLKDFLDISKVDHSYFMIEMNLMNCFQWVDNKELQKLITSWMQKMLF 963

Query: 965  RKGAFNIMIPGSEIPDWFTT-KMGSSISVKWDPNSPNAHLIRFALCVLCGPIDDQDDMTD 1024
            RKGAFNI++PGSEIPDWFTT KMGSSI V+WD ++PNA+++RFALCV+CGP  ++ D+ D
Sbjct: 964  RKGAFNILVPGSEIPDWFTTRKMGSSICVEWDHDAPNANMVRFALCVICGP-SNKKDILD 1023

Query: 1025 VSGSIIASVTGKSHEDSNLKNGDLLVNGFTISGMRKLDHIWLFVLPRTRSLTRKINKCKE 1084
            V  +I ASVTGK   D NL NGDL+V  F +SGM+KLDHIW+FVLPRT +LTRKI  CKE
Sbjct: 1024 VPFTIFASVTGKDRNDPNLNNGDLIVGAFNVSGMKKLDHIWMFVLPRTTTLTRKIRNCKE 1083

Query: 1085 IEFRFLFQANYGQSVTSNIELKKCGVGLINMEKEKEAMKCYASRIILKNKIR 1136
            IEFRFL Q NY Q+V  N+ELKKCGVGLINME+E EAMK YAS II+KN+++
Sbjct: 1084 IEFRFLLQFNYNQTVIPNVELKKCGVGLINMEEESEAMKRYASYIIVKNRMK 1119

BLAST of Lcy12g002500 vs. ExPASy TrEMBL
Match: A0A6J1CL32 (TMV resistance protein N-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111012132 PE=4 SV=1)

HSP 1 Score: 1645.6 bits (4260), Expect = 0.0e+00
Identity = 847/1146 (73.91%), Postives = 957/1146 (83.51%), Query Frame = 0

Query: 1    MVVSSSCSSSGKWKYDVFLSFRGEDTRGGFTDHLYKALVRKG-IFTFRDEDEIKEGKGIS 60
            M VSSS SS+GK+KYDVFLSFRGEDTRGGFTDHLY AL R G IFTFRDE+EI++G  IS
Sbjct: 6    MAVSSSSSSTGKYKYDVFLSFRGEDTRGGFTDHLYAALKRNGIIFTFRDENEIQQGADIS 65

Query: 61   SDLLAAIEASRFALVVVSQNYASSRWCLEELVKIFDCQGRLRMSVLPVFYKVDPSDVRNQ 120
            SDL AAIEASR A+VVVS+NYASSRWCL EL++IF+C+ RL M+VLP+FYKVDPSDVRNQ
Sbjct: 66   SDLSAAIEASRIAVVVVSENYASSRWCLGELLEIFECRKRLGMTVLPIFYKVDPSDVRNQ 125

Query: 121  RGSFGEAFVKHELRFGEDDSKIRKWRQALTKLANLKAWLSQPWSHESKIIEEITEAIWKR 180
            RGSF EAF KH  RFGE+D ++ KWRQALT+LANLKAWLSQ WSHES+IIEEI+ +IWKR
Sbjct: 126  RGSFAEAFAKHGERFGEEDFRLTKWRQALTQLANLKAWLSQHWSHESEIIEEISASIWKR 185

Query: 181  IKPTLTVTQEDQLVGINSKLNKLFSLLNPNLVDD----VVCVGIHGMGGIGKTTIARVCY 240
            IKPTLT+T+EDQL+GINSKL+KL SLLN N   D    V  VGIHGMGGIGKTTIARV Y
Sbjct: 186  IKPTLTLTREDQLLGINSKLDKLSSLLNRNSNSDDDDGVFWVGIHGMGGIGKTTIARVFY 245

Query: 241  ERIRDEFEAHCFLSDVREKFETSGLPHLQSKLLSRM-FSIKNNDIWDVEEGIAMINRAIF 300
            E+IRDEF+AHCFLSDVREKFETSGL +L SKLLSR+ FS+KNNDIWD EEGIAMI+RAIF
Sbjct: 246  EQIRDEFDAHCFLSDVREKFETSGLTYLHSKLLSRLTFSMKNNDIWDAEEGIAMISRAIF 305

Query: 301  QKKTLLVLDDVDSSDQITGLIPNKGSFGSGSRIIITTRNADLLSNEFEVKRIFEVEELQY 360
            QKKTLLVLDDVD SDQI GLIP+   FG GSRIIITTRNADLLSNE  VKRI E+EEL+Y
Sbjct: 306  QKKTLLVLDDVDHSDQIKGLIPSNDFFGYGSRIIITTRNADLLSNELGVKRILEIEELKY 365

Query: 361  EEALQLLNLSAFTKTCPKECFLEHSKSIVKVVGGHPLALKLLGSSLRNKDLSVWNDVIEE 420
            EEALQ L+LS F KTCP+E F EH KSI+K+VGGHPL LKLLG+ LRNKDL VW+ VIEE
Sbjct: 366  EEALQHLSLSVFMKTCPEEGFXEHCKSIIKLVGGHPLGLKLLGTCLRNKDLGVWDCVIEE 425

Query: 421  LQGGGNIHDKIFKCLKVSYDGLDEWEKEIFLDVACFFKGKRRELVEEMLNGCGFYAKRRV 480
            LQ GGNI ++IFKCLKVSYDGLDE EK+IFLDVACFFKGKRR+ VEE+L+GCGFYA+RR+
Sbjct: 426  LQEGGNIDNQIFKCLKVSYDGLDEREKDIFLDVACFFKGKRRDFVEEILDGCGFYARRRI 485

Query: 481  ELLIQKSLLTLSYDNRLHMHDLLQEMGRKIVRHKPI-QDRLWCHKDIKSVVREALVQSIF 540
            ELLIQKSL+TLSYDN+L MHDLLQEMGRKIVR KP  QDRLWCHKDIK VV EALVQSI+
Sbjct: 486  ELLIQKSLITLSYDNKLQMHDLLQEMGRKIVREKPTQQDRLWCHKDIKCVVTEALVQSIY 545

Query: 541  FKSTRIVVEFPIQFSKMHQLRLLNFKNVRLKSQLEYCIPTELRYLKWKGYPMEFLPLSSE 600
            FKS R  VE PI FS+M QLRLLNF NVRL++ LEYCIPTELRYLKWK YP+EFL LSSE
Sbjct: 546  FKSPRNTVEVPILFSRMQQLRLLNFHNVRLQNHLEYCIPTELRYLKWKRYPLEFLRLSSE 605

Query: 601  EYKLIGLHMCHSNLKQFWQGEKHLEELKYIKLNHSQKLSKTPDFTKIPNLERLELEGCTS 660
            EYKLI LH CHSNLKQFW  EKHLE+LKYIKLNHSQKLSKTPDF KIPNL+RLELEGCTS
Sbjct: 606  EYKLITLHXCHSNLKQFWPVEKHLEKLKYIKLNHSQKLSKTPDFAKIPNLKRLELEGCTS 665

Query: 661  LVNIHPSIFLAEKLIFLSLKDCINLTNLPRDINIKVLQVLILSGCSKVNNIPEFSGNTNR 720
            LVNIHPSIF AEKLIFLSL+DC NLTNLP  INIKVL+VLILSGCSK+N +PEFSGNTNR
Sbjct: 666  LVNIHPSIFSAEKLIFLSLRDCTNLTNLPSHINIKVLEVLILSGCSKLNKVPEFSGNTNR 725

Query: 721  LLQLHLDGTSISNLPPSIATLTHLKVLSLKNCSKLINIPNAIDKMKALQSLNLSGCSNLA 780
            LLQLH D TSI+NLP S+A+L  L VLSLKNC KL+NI + IDKM +L+SLNLSGCS L 
Sbjct: 726  LLQLHFDATSIANLPSSVASLNRLTVLSLKNCKKLVNICSVIDKMTSLESLNLSGCSKLG 785

Query: 781  NRKRKASE--AELEELDIRGIARRRRRRDDNDNDNQLVGLNIFRKLFFWLCKAPASCIFG 840
            NRKRK  +    L ELD+RG  RRR       +D   VG+NIF++LF WLCKAP S IFG
Sbjct: 786  NRKRKIDDNVGCLAELDVRGAPRRR-------DDEGKVGVNIFKQLFLWLCKAPESGIFG 845

Query: 841  IPSLAGLYSLTRLNLRDCNLEVIPRGIECLVSLTELNLCGNNFSHLPTSISQLHNLKRLN 900
            +PSLA                             EL LCGNNFSHLPTSIS+LHNL+RLN
Sbjct: 846  VPSLA-----------------------------ELRLCGNNFSHLPTSISRLHNLRRLN 905

Query: 901  INQCKKLVHFPELPPRILRLLSKDCVSLKSIPDISKVEHLYFLKELNLMNCYQLANNKEL 960
            IN CKKL+ FPELPPRI  L+SK C+SLK+IP+ISKV+H YF++E+NL+NCYQLA+N  L
Sbjct: 906  INGCKKLLCFPELPPRIRWLMSKGCISLKNIPNISKVDHSYFMREVNLLNCYQLADNIGL 965

Query: 961  HRLIISWMEKMLFRKGAFNIMIPGSEIPDWFT-TKMGSSISVKWDPNSPNAHLIRFALCV 1020
            HRLI  WMEKMLFRKG FNIMIPGS+IPDWFT T+MGSSI V+WDP++ NA+LIRFALCV
Sbjct: 966  HRLITYWMEKMLFRKGTFNIMIPGSQIPDWFTMTEMGSSICVEWDPDAANANLIRFALCV 1025

Query: 1021 LCGPIDDQDDMTDVSGSIIASVTGKSHEDSNLKNGDLLVNGFTISGMRKLDHIWLFVLPR 1080
            +CGP  +Q D+ D   +IIASVTG+  +D  L NGDL+VN F +SGMRKLDHIWLFVLPR
Sbjct: 1026 VCGP-TNQGDIIDTPFAIIASVTGRGADDPCLNNGDLIVNAFNVSGMRKLDHIWLFVLPR 1085

Query: 1081 TRSLTRKINKCKEIEFRFLFQANYGQSVTS-NIELKKCGVGLINMEKEKEAMKCYASRII 1136
            T+SL RKI+ CKEI+F+FL Q NY +S +S +I LKKCGV LINME+EKEAMK YAS II
Sbjct: 1086 TKSLARKISTCKEIKFQFLVQVNYSRSASSPSIALKKCGVHLINMEEEKEAMKRYASYII 1114

BLAST of Lcy12g002500 vs. NCBI nr
Match: XP_008459550.1 (PREDICTED: TMV resistance protein N-like [Cucumis melo])

HSP 1 Score: 1735.3 bits (4493), Expect = 0.0e+00
Identity = 882/1141 (77.30%), Postives = 994/1141 (87.12%), Query Frame = 0

Query: 4    SSSCSSSGKWKYDVFLSFRGEDTRGGFTDHLYKALVRKGIFTFRDEDEIKEGKGISSDLL 63
            SSS SS GKWK+DVFLSFRGEDTRGGFTDHLYKAL +KGI TFRDE+EI+EG+ ISS+LL
Sbjct: 7    SSSSSSIGKWKFDVFLSFRGEDTRGGFTDHLYKALTQKGISTFRDENEIQEGEDISSNLL 66

Query: 64   AAIEASRFALVVVSQNYASSRWCLEELVKIFDCQGRLRMSVLPVFYKVDPSDVRNQRGSF 123
             +IEASRFA+VVVS+NYASSRWCLEELVKIF+C+ +L M VLP+FYKVDPS VRNQRG F
Sbjct: 67   DSIEASRFAIVVVSENYASSRWCLEELVKIFECEEKLGMDVLPIFYKVDPSHVRNQRGRF 126

Query: 124  GEAFVKHELRFGEDDSKIRKWRQALTKLANLKAWLSQPWSHESKIIEEITEAIWKRIKPT 183
             EAFVKHE+RFG DD K++KWR+ LTKLANLKAWLSQ WSHES IIEEIT  IWKR+K  
Sbjct: 127  EEAFVKHEMRFGRDDIKVQKWRRLLTKLANLKAWLSQSWSHESNIIEEITTTIWKRLKHN 186

Query: 184  LTVTQEDQLVGINSKLNKLFSLLNPNL----VDDVVCVGIHGMGGIGKTTIARVCYERIR 243
            LTV +EDQLVGINSKLNKL SLL PN      DDV+ VGIHGMGGIGKTTIA+VCY+RIR
Sbjct: 187  LTVIKEDQLVGINSKLNKLSSLLIPNSDEDEDDDVIFVGIHGMGGIGKTTIAKVCYQRIR 246

Query: 244  DEFEAHCFLSDVREK-FETSG-LPHLQSKLLSRMFSIKNNDIWDVEEGIAMINRAIFQKK 303
            DEFEAHCFLSDVRE  F TSG LP+LQ+KLLSRMFS KNN I DVEEGIAMIN+AIF+KK
Sbjct: 247  DEFEAHCFLSDVRENYFRTSGDLPYLQTKLLSRMFSFKNNHILDVEEGIAMINKAIFRKK 306

Query: 304  TLLVLDDVDSSDQITGLIPNKGSFGSGSRIIITTRNADLLSNEFEVKRIFEVEELQYEEA 363
            TLLVLDDVD SDQI GLIPNK SFG+GSRIIITTRNADLLSNEF VKRIFE++EL+YEEA
Sbjct: 307  TLLVLDDVDCSDQIMGLIPNKSSFGNGSRIIITTRNADLLSNEFGVKRIFEMDELKYEEA 366

Query: 364  LQLLNLSAFTKTCPKECFLEHSKSIVKVVGGHPLALKLLGSSLRNKDLSVWNDVIEELQG 423
            LQLL+LSAF KTCPKE +LEHSK IVKVVGGHPLALKLLGSSLRNK+LSVWN+VIEE++G
Sbjct: 367  LQLLSLSAFMKTCPKEGYLEHSKKIVKVVGGHPLALKLLGSSLRNKNLSVWNEVIEEVEG 426

Query: 424  GGNIHDKIFKCLKVSYDGLDEWEKEIFLDVACFFKGKRRELVEEMLNGCGFYAKRRVELL 483
            GGNIH+KIFKCLKVSYDGLDEWEKEIFLDVACFF GKRRE+VEE+LNGCGFYAK R+ELL
Sbjct: 427  GGNIHEKIFKCLKVSYDGLDEWEKEIFLDVACFFNGKRREVVEEILNGCGFYAKTRIELL 486

Query: 484  IQKSLLTLSYDNRLHMHDLLQEMGRKIVRHKPIQDRLWCHKDIKSVVREALVQSIFFK-S 543
            IQKSLLTLSYDN+LHMHDLLQEMGRKIVR K ++DRL CHKDIKSVV E LVQSIFFK S
Sbjct: 487  IQKSLLTLSYDNKLHMHDLLQEMGRKIVRDKHVRDRLMCHKDIKSVVTETLVQSIFFKSS 546

Query: 544  TRIVVEFPIQFSKMHQLRLLNFKNVRLKSQLEYCIPTELRYLKWKGYPMEFLPL-SSEEY 603
            ++ +VEFPI FS+MHQLRLLNF NVRLK++LEYCIP+ELRYLKWK YP+EFLP+ SSEE 
Sbjct: 547  SKNMVEFPILFSRMHQLRLLNFHNVRLKNKLEYCIPSELRYLKWKEYPLEFLPINSSEEC 606

Query: 604  KLIGLHMCHSNLKQFWQGEKHLEELKYIKLNHSQKLSKTPDFTKIPNLERLELEGCTSLV 663
            KLI LHMCHSNLKQFWQ EK+L  LKYIKLN SQKLSKTP+F  IPNL+RLELE CTSLV
Sbjct: 607  KLIELHMCHSNLKQFWQQEKNLVGLKYIKLNSSQKLSKTPNFANIPNLKRLELEDCTSLV 666

Query: 664  NIHPSIFLAEKLIFLSLKDCINLTNLPRDINIKVLQVLILSGCSKVNNIPEFSGNTNRLL 723
            NIHPSIF AEKLIFL+LKDCINLTNLP  INIKVL+VLILSGCSKV  +PEFSGNTNRLL
Sbjct: 667  NIHPSIFTAEKLIFLNLKDCINLTNLPSRINIKVLEVLILSGCSKVKKVPEFSGNTNRLL 726

Query: 724  QLHLDGTSISNLPPSIATLTHLKVLSLKNCSKLINIPNAIDKMKALQSLNLSGCSNLANR 783
            QLHLDGTSISNLP SIA+L+HL +LSL NC KLINI NA++ M +LQSL++SGC  L +R
Sbjct: 727  QLHLDGTSISNLPSSIASLSHLTILSLANCKKLINISNAME-MTSLQSLDVSGCLKLGSR 786

Query: 784  KRKASEAELEELDIRGIARRRRRRDDNDNDNQLVGLNIFRKLFFWLCKAPASCIFGIPSL 843
            KRKA + EL ELD+R   RRRR  D N         NIF+K+F WLCK PAS IFGIPSL
Sbjct: 787  KRKADDGELGELDVRETTRRRRNDDSN---------NIFKKIFLWLCKTPASGIFGIPSL 846

Query: 844  AGLYSLTRLNLRDCNLEVIPRGIECLVSLTELNLCGNNFSHLPTSISQLHNLKRLNINQC 903
            AGLYSLT+LNLRDCNLE IP+GIECLVSL EL+L GN+FSHLPTSIS+LHNLK+L INQC
Sbjct: 847  AGLYSLTKLNLRDCNLEEIPQGIECLVSLVELDLSGNSFSHLPTSISRLHNLKKLRINQC 906

Query: 904  KKLVHFPELPPRILRLLSKDCVSLKSIPDISKVEHLYFLKELNLMNCYQLANNKELHRLI 963
             KLV FP+LPPRIL L+SKDC+SLK   DISKV++LY +KE+NL+NCYQLANNK  HRLI
Sbjct: 907  IKLVQFPKLPPRILFLMSKDCISLKDFVDISKVDNLYIMKEVNLLNCYQLANNKGFHRLI 966

Query: 964  ISWMEKMLFRKGAFNIMIPGSEIPDWFTT-KMGSSISVKWDPNSPNAHLIRFALCVLCGP 1023
            ISWM+KMLFRKG FNIMIPGSEIPDWFTT KMGSS+ ++WDP+ PN ++IRFALCV+ G 
Sbjct: 967  ISWMQKMLFRKGTFNIMIPGSEIPDWFTTRKMGSSVCIEWDPDGPNTNMIRFALCVVFGL 1026

Query: 1024 IDDQDDMTDVSGSIIASVTGKSHEDSNLKN-GDLLVNGFTISGMRKLDHIWLFVLPRTRS 1083
             +  D +   S +IIASVTGK   DSNLKN GDLL+ GF ++GM+KLDHIW+FVLPRT +
Sbjct: 1027 SEKIDVVNVPSFAIIASVTGKDRNDSNLKNGGDLLIGGFPVAGMKKLDHIWMFVLPRTGT 1086

Query: 1084 LTRKINKCKEIEFRFLFQ-ANYGQSVTSNIELKKCGVGLINMEKEKEAMKCYASRIILKN 1134
            L RKI+  KEI+FRFL Q ANY QS+T N+++K+CGVGLIN+E+EKEAMK YAS IIL+N
Sbjct: 1087 LVRKISNYKEIKFRFLLQAANYRQSITPNVKVKECGVGLINLEEEKEAMKRYASHIILRN 1137

BLAST of Lcy12g002500 vs. NCBI nr
Match: KAG6592347.1 (Disease resistance protein RUN1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1716.4 bits (4444), Expect = 0.0e+00
Identity = 856/1132 (75.62%), Postives = 969/1132 (85.60%), Query Frame = 0

Query: 5    SSCSSSGKWKYDVFLSFRGEDTRGGFTDHLYKALVRKGIFTFRDEDEIKEGKGISSDLLA 64
            SS SS+GKWK++VFLSFRGEDTR GFTD LY AL+ KGI TFRDEDEI+EG  IS+DL A
Sbjct: 4    SSGSSTGKWKFNVFLSFRGEDTRFGFTDRLYNALIHKGISTFRDEDEIEEGSDISTDLSA 63

Query: 65   AIEASRFALVVVSQNYASSRWCLEELVKIFDCQGRLRMSVLPVFYKVDPSDVRNQRGSFG 124
            AIEASR ALVVVS+NYASSRWCLEEL KIF+C  RL M+VLP+FYKVDPS VRNQ G+F 
Sbjct: 64   AIEASRIALVVVSENYASSRWCLEELSKIFECHHRLGMTVLPIFYKVDPSHVRNQTGTFA 123

Query: 125  EAFVKHELRFGEDDSKIRKWRQALTKLANLKAWLSQPWSHESKIIEEITEAIWKRIKPTL 184
            EAF KHELRFGE +  I+KWR  LTKLANLKAWL +PW+HESK+IEEIT  +WKRIKPTL
Sbjct: 124  EAFAKHELRFGEHNPNIQKWRHLLTKLANLKAWLLEPWTHESKVIEEITTVLWKRIKPTL 183

Query: 185  TVTQEDQLVGINSKLNKLFSLLNPNLVDDVVCVGIHGMGGIGKTTIARVCYERIRDEFEA 244
             VTQE QLVGINSKL KL SLLNPN  +DV+ VGIHGMGGIGKTT+ARVCYERIRD+FEA
Sbjct: 184  IVTQEHQLVGINSKLTKLSSLLNPNSDEDVIWVGIHGMGGIGKTTLARVCYERIRDKFEA 243

Query: 245  HCFLSDVREKFETSGLPHLQSKLLSRMFSIKNNDIWDVEEGIAMINRAIFQKKTLLVLDD 304
            HCF+S+V+EKFETSGLP+LQS+LLSRMFSI+N DI DVEEGIAMIN+A+F+KK LLVLDD
Sbjct: 244  HCFVSNVQEKFETSGLPYLQSQLLSRMFSIENKDIGDVEEGIAMINQAVFRKKILLVLDD 303

Query: 305  VDSSDQITGLIPNKGSFGSGSRIIITTRNADLLSNEFEVKRIFEVEELQYEEALQLLNLS 364
            V+ SDQI GLIPNK SFG+GSRIIITTRNADLLSNEFEVKR+F++ EL  EEALQLLNLS
Sbjct: 304  VNCSDQIMGLIPNKDSFGNGSRIIITTRNADLLSNEFEVKRMFKMVELTNEEALQLLNLS 363

Query: 365  AFTKTCPKECFLEHSKSIVKVVGGHPLALKLLGSSLRNKDLSVWNDVIEELQGGGNIHDK 424
            A    CPK+  LEHSK+IVK+VGGHPLALKLLGSSLRNKDL VW  VIEEL+GGGNIHDK
Sbjct: 364  A----CPKQDCLEHSKNIVKIVGGHPLALKLLGSSLRNKDLKVWKYVIEELKGGGNIHDK 423

Query: 425  IFKCLKVSYDGLDEWEKEIFLDVACFFKGKRRELVEEMLNGCGFYAKRRVELLIQKSLLT 484
            +FKCLKVSYDGLDEWEKEIFLD+ACFFKGKR+ELVEE+LNGCGF+AK RVELLIQKSLLT
Sbjct: 424  VFKCLKVSYDGLDEWEKEIFLDLACFFKGKRKELVEEILNGCGFHAKIRVELLIQKSLLT 483

Query: 485  LSYDNRLHMHDLLQEMGRKIVRHKPIQDRLWCHKDIKSVVREALVQSIFFKSTRIVVEFP 544
            LSY N+L MHDLLQEMGRKIVRHKP+Q+RLW  KDIKS+V EA V+SI FKSTR V+EFP
Sbjct: 484  LSYQNKLMMHDLLQEMGRKIVRHKPVQERLWRQKDIKSMVGEASVESILFKSTRNVMEFP 543

Query: 545  IQFSKMHQLRLLNFKNVRLKSQLEYCIPTELRYLKWKGYPMEFLPLSSEEYKLIGLHMCH 604
            I FS+MHQLRLLNF NVRLK++LEYCIP+ELRYLKWKGYP+E L L+SEE KLI LHMCH
Sbjct: 544  ISFSRMHQLRLLNFHNVRLKNELEYCIPSELRYLKWKGYPLEILVLNSEECKLIKLHMCH 603

Query: 605  SNLKQFWQGEKHLEELKYIKLNHSQKLSKTPDFTKIPNLERLELEGCTSLVNIHPSIFLA 664
            SNLKQFW GEKHLEELKYIKLNHS KLSKTP+F  IPNL RLELEGCTSLVNIHP+IF A
Sbjct: 604  SNLKQFWHGEKHLEELKYIKLNHSPKLSKTPNFETIPNLTRLELEGCTSLVNIHPTIFTA 663

Query: 665  EKLIFLSLKDCINLTNLPRDINIKVLQVLILSGCSKVNNIPEFSGNTNRLLQLHLDGTSI 724
            +KL FLSLKDCINLTN P  INIK L++LIL+GCSK+  IPEFSGNT+ LL+LHLDGTSI
Sbjct: 664  KKLTFLSLKDCINLTNFPPQINIKALEILILNGCSKLKKIPEFSGNTDILLELHLDGTSI 723

Query: 725  SNLPPSIATLTHLKVLSLKNCSKLINIPNAIDKMKALQSLNLSGCSNLANRKRKASEAEL 784
            S+LP SIA L HL VLSL NC  LINI NA+DK+ +L+SLNLSGCS L NRKRK  + E 
Sbjct: 724  SSLPSSIAILDHLTVLSLTNCKNLINISNALDKITSLKSLNLSGCSKLGNRKRKRGDVET 783

Query: 785  EELDIRGIARRRRRRDDNDNDNQLVGLNIFRKLFFWLCKAPASCIFGIPSLAGLYSLTRL 844
             E D+R   R  RR DD D        NIFRK+  WLCKAP S IFGIPSLAGLYSLTRL
Sbjct: 784  VEFDVR---RTARRTDDGD--------NIFRKIILWLCKAPTSGIFGIPSLAGLYSLTRL 843

Query: 845  NLRDCNLEVIPRGIECLVSLTELNLCGNNFSHLPTSISQLHNLKRLNINQCKKLVHFPEL 904
            NL DC LE +P GIECLVSL  LNL  NNFS LPTSIS+LHNLKRLN+N+C+KL+HFPEL
Sbjct: 844  NLSDCKLEEVPEGIECLVSLVNLNLSRNNFSRLPTSISRLHNLKRLNVNECEKLLHFPEL 903

Query: 905  PPRILRLLSKDCVSLKSIPDISKVEHLYFLKELNLMNCYQLANNKELHRLIISWMEKMLF 964
            PPRILRL+SK C+SLK   DISKV+H YF+ E+NLMNC+Q  +NKELH+LI SWM+KMLF
Sbjct: 904  PPRILRLMSKGCISLKDFLDISKVDHSYFMIEMNLMNCFQWVDNKELHKLITSWMQKMLF 963

Query: 965  RKGAFNIMIPGSEIPDWFTT-KMGSSISVKWDPNSPNAHLIRFALCVLCGPIDDQDDMTD 1024
            RKGAFNI++PGSEIPDWFTT KMGSSI V+WD ++PNA+++RFALCV+CGP  ++ D+ D
Sbjct: 964  RKGAFNILVPGSEIPDWFTTKKMGSSICVEWDHDAPNANMVRFALCVICGP-SNKKDILD 1023

Query: 1025 VSGSIIASVTGKSHEDSNLKNGDLLVNGFTISGMRKLDHIWLFVLPRTRSLTRKINKCKE 1084
            V  +I ASVTGK   D NL NGDL+V  F +SGM+KLDHIW+FVLPRT +LTRKI  CKE
Sbjct: 1024 VPFTIFASVTGKDRNDPNLNNGDLIVGAFNVSGMKKLDHIWMFVLPRTTTLTRKIRNCKE 1083

Query: 1085 IEFRFLFQANYGQSVTSNIELKKCGVGLINMEKEKEAMKCYASRIILKNKIR 1136
            IEFRFL Q NY Q+V  N+ELKKCGVGLINME+E EAMK YAS II+KN+++
Sbjct: 1084 IEFRFLLQFNYNQTVIPNVELKKCGVGLINMEEESEAMKRYASYIIVKNRMK 1119

BLAST of Lcy12g002500 vs. NCBI nr
Match: XP_022141876.1 (TMV resistance protein N-like isoform X1 [Momordica charantia])

HSP 1 Score: 1714.1 bits (4438), Expect = 0.0e+00
Identity = 872/1146 (76.09%), Postives = 985/1146 (85.95%), Query Frame = 0

Query: 1    MVVSSSCSSSGKWKYDVFLSFRGEDTRGGFTDHLYKALVRKG-IFTFRDEDEIKEGKGIS 60
            M VSSS SS+GK+KYDVFLSFRGEDTRGGFTDHLY AL R G IFTFRDE+EI++G  IS
Sbjct: 6    MAVSSSSSSTGKYKYDVFLSFRGEDTRGGFTDHLYAALKRNGIIFTFRDENEIQQGADIS 65

Query: 61   SDLLAAIEASRFALVVVSQNYASSRWCLEELVKIFDCQGRLRMSVLPVFYKVDPSDVRNQ 120
            SDL AAIEASR A+VVVS+NYASSRWCL EL++IF+C+ RL M+VLP+FYKVDPSDVRNQ
Sbjct: 66   SDLSAAIEASRIAVVVVSENYASSRWCLGELLEIFECRKRLGMTVLPIFYKVDPSDVRNQ 125

Query: 121  RGSFGEAFVKHELRFGEDDSKIRKWRQALTKLANLKAWLSQPWSHESKIIEEITEAIWKR 180
            RGSF EAF KH  RFGE+D ++ KWRQALT+LANLKAWLSQ WSHES+IIEEI+ +IWKR
Sbjct: 126  RGSFAEAFAKHGERFGEEDFRLTKWRQALTQLANLKAWLSQHWSHESEIIEEISASIWKR 185

Query: 181  IKPTLTVTQEDQLVGINSKLNKLFSLLNPNLVDD----VVCVGIHGMGGIGKTTIARVCY 240
            IKPTLT+T+EDQL+GINSKL+KL SLLN N   D    V  VGIHGMGGIGKTTIARV Y
Sbjct: 186  IKPTLTLTREDQLLGINSKLDKLSSLLNRNSNSDDDDGVFWVGIHGMGGIGKTTIARVFY 245

Query: 241  ERIRDEFEAHCFLSDVREKFETSGLPHLQSKLLSRM-FSIKNNDIWDVEEGIAMINRAIF 300
            E+IRDEF+AHCFLSDVREKFETSGL +L SKLLSR+ FS+KNNDIWD EEGIAMI+RAIF
Sbjct: 246  EQIRDEFDAHCFLSDVREKFETSGLTYLHSKLLSRLTFSMKNNDIWDAEEGIAMISRAIF 305

Query: 301  QKKTLLVLDDVDSSDQITGLIPNKGSFGSGSRIIITTRNADLLSNEFEVKRIFEVEELQY 360
            QKKTLLVLDDVD SDQI GLIP+   FG GSRIIITTRNADLLSNE  VKRI E+EEL+Y
Sbjct: 306  QKKTLLVLDDVDHSDQIKGLIPSNDFFGYGSRIIITTRNADLLSNELGVKRILEIEELKY 365

Query: 361  EEALQLLNLSAFTKTCPKECFLEHSKSIVKVVGGHPLALKLLGSSLRNKDLSVWNDVIEE 420
            EEALQ L+LS F KTCP+E F EH KSI+K+VGGHPL LKLLG+ LRNKDL VW+ VIEE
Sbjct: 366  EEALQHLSLSVFMKTCPEEGFXEHCKSIIKLVGGHPLGLKLLGTCLRNKDLGVWDCVIEE 425

Query: 421  LQGGGNIHDKIFKCLKVSYDGLDEWEKEIFLDVACFFKGKRRELVEEMLNGCGFYAKRRV 480
            LQ GGNI ++IFKCLKVSYDGLDE EK+IFLDVACFFKGKRR+ VEE+L+GCGFYA+RR+
Sbjct: 426  LQEGGNIDNQIFKCLKVSYDGLDEREKDIFLDVACFFKGKRRDFVEEILDGCGFYARRRI 485

Query: 481  ELLIQKSLLTLSYDNRLHMHDLLQEMGRKIVRHKPI-QDRLWCHKDIKSVVREALVQSIF 540
            ELLIQKSL+TLSYDN+L MHDLLQEMGRKIVR KP  QDRLWCHKDIK VV EALVQSI+
Sbjct: 486  ELLIQKSLITLSYDNKLQMHDLLQEMGRKIVREKPTQQDRLWCHKDIKCVVTEALVQSIY 545

Query: 541  FKSTRIVVEFPIQFSKMHQLRLLNFKNVRLKSQLEYCIPTELRYLKWKGYPMEFLPLSSE 600
            FKS R  VE PI FS+M QLRLLNF NVRL++ LEYCIPTELRYLKWK YP+EFL LSSE
Sbjct: 546  FKSPRNTVEVPILFSRMQQLRLLNFHNVRLQNHLEYCIPTELRYLKWKRYPLEFLRLSSE 605

Query: 601  EYKLIGLHMCHSNLKQFWQGEKHLEELKYIKLNHSQKLSKTPDFTKIPNLERLELEGCTS 660
            EYKLI LH CHSNLKQFW  EKHLE+LKYIKLNHSQKLSKTPDF KIPNL+RLELEGCTS
Sbjct: 606  EYKLITLHXCHSNLKQFWPVEKHLEKLKYIKLNHSQKLSKTPDFAKIPNLKRLELEGCTS 665

Query: 661  LVNIHPSIFLAEKLIFLSLKDCINLTNLPRDINIKVLQVLILSGCSKVNNIPEFSGNTNR 720
            LVNIHPSIF AEKLIFLSL+DC NLTNLP  INIKVL+VLILSGCSK+N +PEFSGNTNR
Sbjct: 666  LVNIHPSIFSAEKLIFLSLRDCTNLTNLPSHINIKVLEVLILSGCSKLNKVPEFSGNTNR 725

Query: 721  LLQLHLDGTSISNLPPSIATLTHLKVLSLKNCSKLINIPNAIDKMKALQSLNLSGCSNLA 780
            LLQLH D TSI+NLP S+A+L  L VLSLKNC KL+NI + IDKM +L+SLNLSGCS L 
Sbjct: 726  LLQLHFDATSIANLPSSVASLNRLTVLSLKNCKKLVNICSVIDKMTSLESLNLSGCSKLG 785

Query: 781  NRKRKASE--AELEELDIRGIARRRRRRDDNDNDNQLVGLNIFRKLFFWLCKAPASCIFG 840
            NRKRK  +    L ELD+RG  RRR       +D   VG+NIF++LF WLCKAP S IFG
Sbjct: 786  NRKRKIDDNVGCLAELDVRGAPRRR-------DDEGKVGVNIFKQLFLWLCKAPESGIFG 845

Query: 841  IPSLAGLYSLTRLNLRDCNLEVIPRGIECLVSLTELNLCGNNFSHLPTSISQLHNLKRLN 900
            +PSLAGLYSLT+LNL+DCNLE IP+GIECLVSLTEL LCGNNFSHLPTSIS+LHNL+RLN
Sbjct: 846  VPSLAGLYSLTKLNLKDCNLETIPQGIECLVSLTELRLCGNNFSHLPTSISRLHNLRRLN 905

Query: 901  INQCKKLVHFPELPPRILRLLSKDCVSLKSIPDISKVEHLYFLKELNLMNCYQLANNKEL 960
            IN CKKL+ FPELPPRI  L+SK C+SLK+IP+ISKV+H YF++E+NL+NCYQLA+N  L
Sbjct: 906  INGCKKLLCFPELPPRIRWLMSKGCISLKNIPNISKVDHSYFMREVNLLNCYQLADNIGL 965

Query: 961  HRLIISWMEKMLFRKGAFNIMIPGSEIPDWFT-TKMGSSISVKWDPNSPNAHLIRFALCV 1020
            HRLI  WMEKMLFRKG FNIMIPGS+IPDWFT T+MGSSI V+WDP++ NA+LIRFALCV
Sbjct: 966  HRLITYWMEKMLFRKGTFNIMIPGSQIPDWFTMTEMGSSICVEWDPDAANANLIRFALCV 1025

Query: 1021 LCGPIDDQDDMTDVSGSIIASVTGKSHEDSNLKNGDLLVNGFTISGMRKLDHIWLFVLPR 1080
            +CGP  +Q D+ D   +IIASVTG+  +D  L NGDL+VN F +SGMRKLDHIWLFVLPR
Sbjct: 1026 VCGP-TNQGDIIDTPFAIIASVTGRGADDPCLNNGDLIVNAFNVSGMRKLDHIWLFVLPR 1085

Query: 1081 TRSLTRKINKCKEIEFRFLFQANYGQSVTS-NIELKKCGVGLINMEKEKEAMKCYASRII 1136
            T+SL RKI+ CKEI+F+FL Q NY +S +S +I LKKCGV LINME+EKEAMK YAS II
Sbjct: 1086 TKSLARKISTCKEIKFQFLVQVNYSRSASSPSIALKKCGVHLINMEEEKEAMKRYASYII 1143

BLAST of Lcy12g002500 vs. NCBI nr
Match: XP_023535476.1 (TMV resistance protein N-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1706.8 bits (4419), Expect = 0.0e+00
Identity = 851/1132 (75.18%), Postives = 966/1132 (85.34%), Query Frame = 0

Query: 5    SSCSSSGKWKYDVFLSFRGEDTRGGFTDHLYKALVRKGIFTFRDEDEIKEGKGISSDLLA 64
            SS SS+GKWK++VFLSFRGEDTR GFTD LYKAL+ KGI TFRDEDEI+EGK IS+DL A
Sbjct: 4    SSGSSTGKWKFNVFLSFRGEDTRFGFTDRLYKALIHKGISTFRDEDEIEEGKDISTDLSA 63

Query: 65   AIEASRFALVVVSQNYASSRWCLEELVKIFDCQGRLRMSVLPVFYKVDPSDVRNQRGSFG 124
            AIEASR AL+VVS+NYASSRWCLEEL KIF+C  RL M+VLP+FYKVDPS VRNQ G+F 
Sbjct: 64   AIEASRIALIVVSENYASSRWCLEELSKIFECHHRLGMTVLPIFYKVDPSHVRNQTGTFA 123

Query: 125  EAFVKHELRFGEDDSKIRKWRQALTKLANLKAWLSQPWSHESKIIEEITEAIWKRIKPTL 184
            E+F KHELRFGE +  I+KWR  LT LANLKAWL +P +HESK+IEEIT  +WKRIKPTL
Sbjct: 124  ESFAKHELRFGEHNPNIQKWRHLLTNLANLKAWLLEPGTHESKVIEEITTVLWKRIKPTL 183

Query: 185  TVTQEDQLVGINSKLNKLFSLLNPNLVDDVVCVGIHGMGGIGKTTIARVCYERIRDEFEA 244
             VTQE QLVGINSKL KL SLLN N  +DV+ +GIHGMGGIGKTT+ARVCYERIRD+FEA
Sbjct: 184  RVTQEHQLVGINSKLTKLSSLLNSNSDEDVIWIGIHGMGGIGKTTLARVCYERIRDKFEA 243

Query: 245  HCFLSDVREKFETSGLPHLQSKLLSRMFSIKNNDIWDVEEGIAMINRAIFQKKTLLVLDD 304
            HCF+S+V+EKFETSGLP+LQS+LLSRMFSI+N DI DVEEGIAMIN+A+FQKK LLVLDD
Sbjct: 244  HCFVSNVQEKFETSGLPYLQSQLLSRMFSIENKDIGDVEEGIAMINQAVFQKKILLVLDD 303

Query: 305  VDSSDQITGLIPNKGSFGSGSRIIITTRNADLLSNEFEVKRIFEVEELQYEEALQLLNLS 364
            V+ SDQI GLIPNK SFG+GSRIIITTRNADLLSNEFEVKR+F++ EL  EEALQLLNLS
Sbjct: 304  VNCSDQIMGLIPNKNSFGNGSRIIITTRNADLLSNEFEVKRMFKMVELTNEEALQLLNLS 363

Query: 365  AFTKTCPKECFLEHSKSIVKVVGGHPLALKLLGSSLRNKDLSVWNDVIEELQGGGNIHDK 424
            A    CPK+  LEHSK+IVK+VGGHPLALKLLGSSLRNKDL VW  VIEEL+GGGNIHDK
Sbjct: 364  A----CPKQDCLEHSKNIVKIVGGHPLALKLLGSSLRNKDLKVWKYVIEELKGGGNIHDK 423

Query: 425  IFKCLKVSYDGLDEWEKEIFLDVACFFKGKRRELVEEMLNGCGFYAKRRVELLIQKSLLT 484
            +FKCLKVSYDGLDEWEKEIFLDVACFFKGKR+ELVEE+LNGCGF+AK RVELLIQKSLLT
Sbjct: 424  VFKCLKVSYDGLDEWEKEIFLDVACFFKGKRKELVEEILNGCGFHAKIRVELLIQKSLLT 483

Query: 485  LSYDNRLHMHDLLQEMGRKIVRHKPIQDRLWCHKDIKSVVREALVQSIFFKSTRIVVEFP 544
            LSY N+L MHDLLQEMGRKIVRHKP+QDRLW  KDIKS+V EA V+SI FKSTR V+EFP
Sbjct: 484  LSYHNKLMMHDLLQEMGRKIVRHKPVQDRLWRQKDIKSMVGEASVESILFKSTRNVMEFP 543

Query: 545  IQFSKMHQLRLLNFKNVRLKSQLEYCIPTELRYLKWKGYPMEFLPLSSEEYKLIGLHMCH 604
            I FS+MHQLRLLNF NVRLK++LEYCIP+ELRYLKWKGYP+E L L+SEE KLI LHMCH
Sbjct: 544  ISFSRMHQLRLLNFHNVRLKNELEYCIPSELRYLKWKGYPLEILLLNSEECKLIKLHMCH 603

Query: 605  SNLKQFWQGEKHLEELKYIKLNHSQKLSKTPDFTKIPNLERLELEGCTSLVNIHPSIFLA 664
            SNLKQFW GEKHLEELKYIKLNHS+KL KTP+F  IPNL RLELEGC SLVNIHP+IF A
Sbjct: 604  SNLKQFWHGEKHLEELKYIKLNHSRKLFKTPNFETIPNLTRLELEGCISLVNIHPTIFTA 663

Query: 665  EKLIFLSLKDCINLTNLPRDINIKVLQVLILSGCSKVNNIPEFSGNTNRLLQLHLDGTSI 724
            +KL FLSLKDCINLTN P  INIK L++LIL+GCSK+  IPEFSGNT+ LL+LHLDGTSI
Sbjct: 664  KKLTFLSLKDCINLTNFPPQINIKALEILILNGCSKLKKIPEFSGNTSILLELHLDGTSI 723

Query: 725  SNLPPSIATLTHLKVLSLKNCSKLINIPNAIDKMKALQSLNLSGCSNLANRKRKASEAEL 784
            S+LP SIA L HL VLSL NC  LINI NA+DK+ +L+SLNLSGCS L NRKRK  + E 
Sbjct: 724  SSLPSSIAILDHLTVLSLTNCKNLINISNALDKITSLKSLNLSGCSKLGNRKRKRGDVET 783

Query: 785  EELDIRGIARRRRRRDDNDNDNQLVGLNIFRKLFFWLCKAPASCIFGIPSLAGLYSLTRL 844
             E D+R   R  RR DD D        NIFRK+  WLCKAP S IFGIPSLAGLYSLTRL
Sbjct: 784  VEFDVR---RTTRRTDDGD--------NIFRKIILWLCKAPTSGIFGIPSLAGLYSLTRL 843

Query: 845  NLRDCNLEVIPRGIECLVSLTELNLCGNNFSHLPTSISQLHNLKRLNINQCKKLVHFPEL 904
            NL DC LE +P GIECLVSL  LNL  NNFSHLPTSIS+LHNLKRLN+N+C+KL+HFP+L
Sbjct: 844  NLSDCKLEEVPEGIECLVSLVHLNLSRNNFSHLPTSISRLHNLKRLNVNECEKLLHFPKL 903

Query: 905  PPRILRLLSKDCVSLKSIPDISKVEHLYFLKELNLMNCYQLANNKELHRLIISWMEKMLF 964
            PPRILRL+SK C+SLK   DISKV+H YF+ E+NLMNC+Q  +NKELH+LI SWM+KMLF
Sbjct: 904  PPRILRLMSKGCISLKDFLDISKVDHSYFMIEMNLMNCFQWVDNKELHKLITSWMQKMLF 963

Query: 965  RKGAFNIMIPGSEIPDWFTT-KMGSSISVKWDPNSPNAHLIRFALCVLCGPIDDQDDMTD 1024
            RKG FNI++PG EIPDWFTT KMGSSI V+WD ++PNA+++RFALCV+CGP  ++ D+ D
Sbjct: 964  RKGPFNILVPGCEIPDWFTTKKMGSSICVEWDHDAPNANMVRFALCVICGP-SNKKDIID 1023

Query: 1025 VSGSIIASVTGKSHEDSNLKNGDLLVNGFTISGMRKLDHIWLFVLPRTRSLTRKINKCKE 1084
            V   I ASVTGK H D NL NGDL+V  F +SGM+KLDHIW+FVLPRT +LTRKI  CKE
Sbjct: 1024 VPFIIFASVTGKDHNDPNLNNGDLIVGAFNVSGMKKLDHIWMFVLPRTTTLTRKIRNCKE 1083

Query: 1085 IEFRFLFQANYGQSVTSNIELKKCGVGLINMEKEKEAMKCYASRIILKNKIR 1136
            IEFRF+ Q NY Q+V  N+ELKKCGVGLINME+E EAMK YAS II+KN+++
Sbjct: 1084 IEFRFVLQFNYNQTVIPNVELKKCGVGLINMEEESEAMKRYASYIIVKNRMK 1119

BLAST of Lcy12g002500 vs. NCBI nr
Match: XP_022973476.1 (TMV resistance protein N-like [Cucurbita maxima])

HSP 1 Score: 1702.6 bits (4408), Expect = 0.0e+00
Identity = 850/1132 (75.09%), Postives = 969/1132 (85.60%), Query Frame = 0

Query: 5    SSCSSSGKWKYDVFLSFRGEDTRGGFTDHLYKALVRKGIFTFRDEDEIKEGKGISSDLLA 64
            SS SS+GKWK++VFLSFRGEDTR GFTD LY AL+ KGI TFRDEDEI+EG  IS+DL A
Sbjct: 4    SSGSSTGKWKFNVFLSFRGEDTRFGFTDRLYNALIHKGISTFRDEDEIEEGADISTDLSA 63

Query: 65   AIEASRFALVVVSQNYASSRWCLEELVKIFDCQGRLRMSVLPVFYKVDPSDVRNQRGSFG 124
            AIEASR ALVVVS+NYASSRWCLEEL KIF+C  RL M+VLP+FYKVDPS VR Q G+F 
Sbjct: 64   AIEASRIALVVVSENYASSRWCLEELSKIFECHHRLGMTVLPIFYKVDPSHVRKQTGTFA 123

Query: 125  EAFVKHELRFGEDDSKIRKWRQALTKLANLKAWLSQPWSHESKIIEEITEAIWKRIKPTL 184
            EAF KHE+RFGE +  I+KWRQ LTKLANLKAWL +PW+HESK+IEEIT+ +WKRIKPTL
Sbjct: 124  EAFAKHEIRFGEHNPNIQKWRQLLTKLANLKAWLLEPWTHESKVIEEITKVLWKRIKPTL 183

Query: 185  TVTQEDQLVGINSKLNKLFSLLNPNLVDDVVCVGIHGMGGIGKTTIARVCYERIRDEFEA 244
             VTQE QLVGINSKL KL SLLNPN  +DV+ +GIHGMGGIGKTT+ARVCYERIRD+FEA
Sbjct: 184  RVTQEHQLVGINSKLTKLSSLLNPNSDEDVIWIGIHGMGGIGKTTLARVCYERIRDKFEA 243

Query: 245  HCFLSDVREKFETSGLPHLQSKLLSRMFSIKNNDIWDVEEGIAMINRAIFQKKTLLVLDD 304
            HCF+S+V+EKFETSGLP+LQS+LLSRMFSI+N DI DVEEGIAMIN+A+F+KK LLVLDD
Sbjct: 244  HCFVSNVQEKFETSGLPYLQSQLLSRMFSIENKDIGDVEEGIAMINQAVFRKKILLVLDD 303

Query: 305  VDSSDQITGLIPNKGSFGSGSRIIITTRNADLLSNEFEVKRIFEVEELQYEEALQLLNLS 364
            V+ SDQI GLIPNK SFG+GSRIIIT RNADLLSNE EVKR+F++ EL  EEALQLLNLS
Sbjct: 304  VNCSDQIMGLIPNKDSFGNGSRIIITARNADLLSNELEVKRMFKMVELTNEEALQLLNLS 363

Query: 365  AFTKTCPKECFLEHSKSIVKVVGGHPLALKLLGSSLRNKDLSVWNDVIEELQGGGNIHDK 424
            A     PK+  LEHSK+IVK+VGGHPLALKLLGSSLRNKDL VW  VIEEL+GGGNIH+K
Sbjct: 364  A----SPKQDCLEHSKNIVKIVGGHPLALKLLGSSLRNKDLKVWKYVIEELKGGGNIHEK 423

Query: 425  IFKCLKVSYDGLDEWEKEIFLDVACFFKGKRRELVEEMLNGCGFYAKRRVELLIQKSLLT 484
            +FKCLKVSYDGLDEWEKEIFLD+ACFFKGKR+ELVEE+L+ CGF+AK RVELLIQKSLLT
Sbjct: 424  VFKCLKVSYDGLDEWEKEIFLDIACFFKGKRKELVEEILHSCGFHAKIRVELLIQKSLLT 483

Query: 485  LSYDNRLHMHDLLQEMGRKIVRHKPIQDRLWCHKDIKSVVREALVQSIFFKSTRIVVEFP 544
            LSY N+L MHDLLQEMG+KIVRHKPIQDRLW  KDIKS+V EA V+SI FKSTR VVEFP
Sbjct: 484  LSYHNKLMMHDLLQEMGQKIVRHKPIQDRLWRQKDIKSMVGEASVESILFKSTRNVVEFP 543

Query: 545  IQFSKMHQLRLLNFKNVRLKSQLEYCIPTELRYLKWKGYPMEFLPLSSEEYKLIGLHMCH 604
            I FS+MHQLRLLNF NVRLK+ LEYCIP+ELRYLKWKGYP+E L L+SEEYKLI LHMCH
Sbjct: 544  ISFSRMHQLRLLNFHNVRLKNVLEYCIPSELRYLKWKGYPLESLVLNSEEYKLIKLHMCH 603

Query: 605  SNLKQFWQGEKHLEELKYIKLNHSQKLSKTPDFTKIPNLERLELEGCTSLVNIHPSIFLA 664
            S+LKQFW GEKHLEELKYIKLNHSQKLSKTP+F  IPNL RLEL GCTSLVNIHP+IF +
Sbjct: 604  SHLKQFWHGEKHLEELKYIKLNHSQKLSKTPNFETIPNLTRLELVGCTSLVNIHPTIFTS 663

Query: 665  EKLIFLSLKDCINLTNLPRDINIKVLQVLILSGCSKVNNIPEFSGNTNRLLQLHLDGTSI 724
            ++L FLSLKDCINLTN P  INIK L+VLILSGCSK+  IPEFSGNT++LL+LHLDGTSI
Sbjct: 664  KELTFLSLKDCINLTNFPPQINIKALEVLILSGCSKLKKIPEFSGNTDKLLELHLDGTSI 723

Query: 725  SNLPPSIATLTHLKVLSLKNCSKLINIPNAIDKMKALQSLNLSGCSNLANRKRKASEAEL 784
            S+LP SIA L HL VLSL NC  LINI NA+DKM +L+SLNLSGCS L NRKRK  + E 
Sbjct: 724  SSLPSSIAILDHLTVLSLTNCKNLINISNALDKMTSLKSLNLSGCSKLGNRKRKRVDVET 783

Query: 785  EELDIRGIARRRRRRDDNDNDNQLVGLNIFRKLFFWLCKAPASCIFGIPSLAGLYSLTRL 844
             ELD+R   R  RR DD D        NIFRK+F WLCKAP S IFGIPSLAGLYSLTRL
Sbjct: 784  VELDVR---RTARRTDDGD--------NIFRKIFLWLCKAPTSGIFGIPSLAGLYSLTRL 843

Query: 845  NLRDCNLEVIPRGIECLVSLTELNLCGNNFSHLPTSISQLHNLKRLNINQCKKLVHFPEL 904
            NL DC LE +P GIECLVSL  LNL  NNFS LPTSIS+LHNLKRLN+N+C+KL+HFP+L
Sbjct: 844  NLSDCKLEEVPEGIECLVSLVNLNLSRNNFSRLPTSISRLHNLKRLNVNECEKLLHFPKL 903

Query: 905  PPRILRLLSKDCVSLKSIPDISKVEHLYFLKELNLMNCYQLANNKELHRLIISWMEKMLF 964
            PPRILRL+SK C+SLK   DISKV+H YF+ E+NL+NC+Q  +NK+LH+LI SWM+KMLF
Sbjct: 904  PPRILRLMSKGCISLKDFLDISKVDHSYFMIEMNLLNCFQWVDNKDLHKLITSWMQKMLF 963

Query: 965  RKGAFNIMIPGSEIPDWFTT-KMGSSISVKWDPNSPNAHLIRFALCVLCGPIDDQDDMTD 1024
            RKGAFNI++PGSEIPDWFTT KMGSSI V+WD ++PNA+++RFALCV+CGP + Q D+ D
Sbjct: 964  RKGAFNILVPGSEIPDWFTTKKMGSSICVEWDHDAPNANMVRFALCVICGPSNKQ-DILD 1023

Query: 1025 VSGSIIASVTGKSHEDSNLKNGDLLVNGFTISGMRKLDHIWLFVLPRTRSLTRKINKCKE 1084
            V  +I ASVTGK+  D N  NGDL+V  F +SGM+KLDHIW+FVLPRT +LTRKI  CKE
Sbjct: 1024 VPVTIFASVTGKNRNDPNSNNGDLIVGAFNVSGMKKLDHIWMFVLPRTTTLTRKIRNCKE 1083

Query: 1085 IEFRFLFQANYGQSVTSNIELKKCGVGLINMEKEKEAMKCYASRIILKNKIR 1136
            IEFRFL Q NY Q+V  N+ELKKCGVGLINME+E EAMK YAS II+KN+++
Sbjct: 1084 IEFRFLLQFNYNQTVIPNVELKKCGVGLINMEEESEAMKRYASYIIVKNRMK 1119

BLAST of Lcy12g002500 vs. TAIR 10
Match: AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 555.4 bits (1430), Expect = 1.0e-157
Identity = 418/1259 (33.20%), Postives = 638/1259 (50.68%), Query Frame = 0

Query: 4    SSSCSSSGKWKYDVFLSFRGEDTRGGFTDHLYKALVRKGIFTFRDEDEIKEGKGISSDLL 63
            SSS SSS  WK DVF+SFRGED R  F  HL+    R GI  FRD+ +++ GK IS +L+
Sbjct: 7    SSSSSSSTVWKTDVFVSFRGEDVRKTFVSHLFCEFDRMGIKAFRDDLDLQRGKSISPELI 66

Query: 64   AAIEASRFALVVVSQNYASSRWCLEELVKIFDCQGRLRMSVLPVFYKVDPSDVRNQRGSF 123
             AI+ SRFA+VVVS+NYA+S WCL+EL+KI +C    + +++P+FY+VDPSDVR QRGSF
Sbjct: 67   DAIKGSRFAIVVVSRNYAASSWCLDELLKIMECN---KDTIVPIFYEVDPSDVRRQRGSF 126

Query: 124  GEAFVKHELRFGEDDSKIRKWRQALTKLANLKAWLSQPWSHESKIIEEITEAIWKRIKPT 183
            GE    H      D  K+ KW++AL KLA +    S+ W  +SK+I++I + I  ++  T
Sbjct: 127  GEDVESH-----SDKEKVGKWKEALKKLAAISGEDSRNWD-DSKLIKKIVKDISDKLVST 186

Query: 184  LTVTQEDQLVGINSKLNKLFSLLNPNLVD-DVVCVGIHGMGGIGKTTIARVCYERIRDEF 243
             +      L+G++S ++ L S++  ++VD DV  +GI GMGG+GKTTIA+  Y ++  +F
Sbjct: 187  -SWDDSKGLIGMSSHMDFLQSMI--SIVDKDVRMLGIWGMGGVGKTTIAKYLYNQLSGQF 246

Query: 244  EAHCFLSDVREKFETSGLPHLQSKLLSRMFSIKNNDIWDVEEGIAMINRAIFQKKTLLVL 303
            + HCF+ +V+E     G+  LQ + L RMF  ++ + W       +I      K   +VL
Sbjct: 247  QVHCFMENVKEVCNRYGVRRLQVEFLCRMFQERDKEAWSSVSCCNIIKERFRHKMVFIVL 306

Query: 304  DDVDSSDQITGLIPNKGSFGSGSRIIITTRNADLLSNEFEVKRIFEVEELQYEEALQLLN 363
            DDVD S+Q+  L+   G FG GSRII+TTR+  LL +   +  +++V+ L  +EALQL  
Sbjct: 307  DDVDRSEQLNELVKETGWFGPGSRIIVTTRDRHLLLSH-GINLVYKVKCLPKKEALQLFC 366

Query: 364  LSAF-TKTCPKECFLEHSKSIVKVVGGHPLALKLLGSSLRNKDLSVWNDVIEELQGGGNI 423
              AF  +      F E S   V    G PLAL++LGS L  +    W   +  L+     
Sbjct: 367  NYAFREEIILPHGFEELSVQAVNYASGLPLALRVLGSFLYRRSQIEWESTLARLK--TYP 426

Query: 424  HDKIFKCLKVSYDGLDEWEKEIFLDVACFFKGKRRELVEEMLNGCGFYAKRRVELLIQKS 483
            H  I + L+VSYDGLDE EK IFL ++CF+  K+ + V ++L+ CG+ A+  + +L +KS
Sbjct: 427  HSDIMEVLRVSYDGLDEQEKAIFLYISCFYNMKQVDYVRKLLDLCGYAAEIGITILTEKS 486

Query: 484  LLTLSYDNRLHMHDLLQEMGRKIVRHKPIQD-----RLWCHKDIKSVVRE----ALVQSI 543
            L+  S +  + +HDLL++MGR++VR + + +      LW  +DI  ++ E     LV+ I
Sbjct: 487  LIVES-NGCVKIHDLLEQMGRELVRQQAVNNPAQRLLLWDPEDICHLLSENSGTQLVEGI 546

Query: 544  FFKSTRIVVEFPIQ--FSKMHQLRLLNF--------KNVRLKSQLEYCIPTELRYLKWKG 603
                + I   F     F  +  L+LLNF          V L + L Y +P +LRYL+W G
Sbjct: 547  SLNLSEISEVFASDRAFEGLSNLKLLNFYDLSFDGETRVHLPNGLSY-LPRKLRYLRWDG 606

Query: 604  YPMEFLPLSSEEYKLIGLHMCHSNLKQFWQGEKHLEELKYIKLNHSQKLSKTPDFTKIPN 663
            YP++ +P       L+ L M +SNL++ W G + L  LK + L+  + L + PD +K  N
Sbjct: 607  YPLKTMPSRFFPEFLVELCMSNSNLEKLWDGIQPLRNLKKMDLSRCKYLVEVPDLSKATN 666

Query: 664  LERLELEGCTSLVNIHPSIFLAEKLIFLSLKDCINLTNLPRDINIKVLQVLILSGCSKVN 723
            LE L L  C SLV + PSI   + L    L +CI L ++P  I +K L+ + +SGCS + 
Sbjct: 667  LEELNLSYCQSLVEVTPSIKNLKGLSCFYLTNCIQLKDIPIGIILKSLETVGMSGCSSLK 726

Query: 724  NIPEFSGNTNRLLQLHLDGTSISNLPPSIATLT------------------------HLK 783
            + PE S NT R   L+L  T I  LP SI+ L+                         LK
Sbjct: 727  HFPEISWNTRR---LYLSSTKIEELPSSISRLSCLVKLDMSDCQRLRTLPSYLGHLVSLK 786

Query: 784  VLSLKNCSKLINIPNAIDKMKALQSLNLSGCSNLANRKR--------KASEAELEEL--- 843
             L+L  C +L N+P+ +  + +L++L +SGC N+    R        + SE  +EE+   
Sbjct: 787  SLNLDGCRRLENLPDTLQNLTSLETLEVSGCLNVNEFPRVSTSIEVLRISETSIEEIPAR 846

Query: 844  --------------------------DIRGIAR--------------------------- 903
                                      ++R + +                           
Sbjct: 847  ICNLSQLRSLDISENKRLASLPVSISELRSLEKLKLSGCSVLESFPLEICQTMSCLRWFD 906

Query: 904  --RRRRRDDNDNDNQLVGLNIFRKLFFWLCKAPASC-------IFGI------------- 963
              R   ++  +N   LV L + +     + +AP S        +  I             
Sbjct: 907  LDRTSIKELPENIGNLVALEVLQASRTVIRRAPWSIARLTRLQVLAIGNSFFTPEGLLHS 966

Query: 964  --PSLAGLYSLTRLNLRDCNLEVIPRGIECLVSLTELNLCGNNFSHLPTSISQLHNLKRL 1023
              P L+    L  L+L + N+  IP  I  L +L EL+L GNNF  +P SI +L  L RL
Sbjct: 967  LCPPLSRFDDLRALSLSNMNMTEIPNSIGNLWNLLELDLSGNNFEFIPASIKRLTRLNRL 1026

Query: 1024 NINQCKKLVHFP-ELPPRILRLLSKDCVSLKSIPDISKVEHLYFLKELNLMNCYQLANNK 1083
            N+N C++L   P ELP  +L +    C SL S   IS   + Y L++L   NCY+L    
Sbjct: 1027 NLNNCQRLQALPDELPRGLLYIYIHSCTSLVS---ISGCFNQYCLRKLVASNCYKL---D 1086

Query: 1084 ELHRLIISWMEKMLFRKGAFNIMIPGSEIPDWFTTK-MGSSISVKWDPNSPNAHLIRFAL 1116
            +  +++I    K+   K   +   PGS+IP  F  + MG S++++   +  ++ ++ F+ 
Sbjct: 1087 QAAQILIHRNLKLESAKPEHS-YFPGSDIPTCFNHQVMGPSLNIQLPQSESSSDILGFSA 1146

BLAST of Lcy12g002500 vs. TAIR 10
Match: AT4G12010.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 552.0 bits (1421), Expect = 1.1e-156
Identity = 401/1198 (33.47%), Postives = 617/1198 (51.50%), Query Frame = 0

Query: 8    SSSGKWKYDVFLSFRGEDTRGGFTDHLYKALVRKGIFTFRDEDEIKEGKGISSDLLAAIE 67
            SS    ++DVFLSFRG DTR  FT HL KAL  +GI +F D D ++ G  +++ L   IE
Sbjct: 4    SSPSSAEFDVFLSFRGFDTRNNFTGHLQKALRLRGIDSFID-DRLRRGDNLTA-LFDRIE 63

Query: 68   ASRFALVVVSQNYASSRWCLEELVKIFDCQGRLRMSVLPVFYKVDPSDVRNQRGSFGEAF 127
             S+ A++V S NYA+S WCL ELVKI +C+   +  V+P+FYKVD SDV  QR SF   F
Sbjct: 64   KSKIAIIVFSTNYANSAWCLRELVKILECRNSNQQLVVPIFYKVDKSDVEKQRNSFAVPF 123

Query: 128  VKHELRF-GEDDSKIRKWRQALTKLANLKAWLSQPWS-HESKIIEEITEAIWKRIKPTLT 187
               EL F G    +I  W+ AL   +N+  ++ +  S  E+K+++EI    +K++   L 
Sbjct: 124  KLPELTFPGVTPEEISSWKAALASASNILGYVVKEISTSEAKLVDEIAVDTFKKLN-DLA 183

Query: 188  VTQEDQLVGINSKLNKLFSLLNPNLVDDVVCVGIHGMGGIGKTTIARVCYERIRDEFEAH 247
             +  + LVGI S+L  L  LL+   +D V  +GI GM GIGKTT+A   Y R+R +F+  
Sbjct: 184  PSGNEGLVGIESRLKNLEKLLSWEDLDTVHIIGIVGMVGIGKTTLADCLYGRMRGQFDGS 243

Query: 248  CFLSDVREKFETSGLPHLQSKLLSRMFSIKNNDIWDVEEGIAMINRAIFQKKTLLVLDDV 307
            CFL+++RE    SGL  L  KL S + + ++ +I           R +  K+ L+VLDDV
Sbjct: 244  CFLTNIRENSGRSGLESLLQKLFSTVLNDRDLEIGAPGNAHERFERRLKSKRLLIVLDDV 303

Query: 308  DSSDQITGLIPNKGSFGSGSRIIITTRNADLLSNEFEVKRIFEVEELQYEEALQLLNLSA 367
            +   QI  L+ +   +  GSRIIITTR++ L+  E    R + + +L   EAL+L +L+A
Sbjct: 304  NDEKQIRYLMGHCKWYQGGSRIIITTRDSKLI--ETIKGRKYVLPKLNDREALKLFSLNA 363

Query: 368  FTKTCPKECFLEHSKSIVKVVGGHPLALKLLGSSLRNKDLSVWNDVIEELQGGGNIHDKI 427
            F+ + P + F   +  ++    GHPLALK+LGS L  +D   W   ++ L+     H  I
Sbjct: 364  FSNSFPLKEFEGLTNMVLDYAKGHPLALKVLGSDLCERDDLYWEAKLDRLK--SRSHGDI 423

Query: 428  FKCLKVSYDGLDEWEKEIFLDVACFFKGKRRELVEEMLNGCGFYAKRRVELLIQKSLLTL 487
            ++ L+ SY+ L   +K +FLD+ACFF+ +  + V  +LN  G      V+ L+ K L+TL
Sbjct: 424  YEVLETSYEELTTEQKNVFLDIACFFRSENVDYVTSLLNSHGVDVSGVVKDLVDKCLITL 483

Query: 488  SYDNRLHMHDLLQEMGRKI---VRHKPIQD---------------RLWCHKDIKSVVREA 547
            S DNR+ MHD+LQ M ++I   V    I+D               RLW  +DI  ++ E 
Sbjct: 484  S-DNRIEMHDMLQTMAKEISLKVETIGIRDCRWLSRHGNQCQWHIRLWDSEDICDLLTEG 543

Query: 548  L----VQSIFFKSTRI-VVEFPIQ-FSKMHQLRLLNFKN------------VRLKSQLEY 607
            L    ++ IF  ++++  +    + F  M+ L+ L   +            + L+  L +
Sbjct: 544  LGTDKIRGIFLDTSKLRAMRLSAKAFQGMYNLKYLKIYDSHCSRGCEAEFKLHLRRGLSF 603

Query: 608  CIPTELRYLKWKGYPMEFLPLSSEEYKLIGLHMCHSNLKQFWQGEKHLEELKYIKLNHSQ 667
             +P EL YL W GYP++ +PL  +   L+ L + HS L++ W  EK +  LK++ L+HS 
Sbjct: 604  -LPNELTYLHWHGYPLQSIPLDFDPKNLVDLKLPHSQLEEIWDDEKDVGMLKWVDLSHSI 663

Query: 668  KLSKTPDFTKIPNLERLELEGCTSLVNIHPSIFLAEKLIFLSLKDCINLTNLPRDINIKV 727
             L +        NLERL LEGCTSL  +  +I   EKLI+L+L+DC +L +LP+ I  + 
Sbjct: 664  NLRQCLGLANAHNLERLNLEGCTSLKKLPSTINCLEKLIYLNLRDCTSLRSLPKGIKTQS 723

Query: 728  LQVLILSGCSKVNNIPEFSGNTNRLLQLHLDGTSISNLPPSIATLTHLKVLSLKNCSKLI 787
            LQ LILSGCS +   P  S N   LL   LDGT I +LP SI T   L +L+LKNC KL 
Sbjct: 724  LQTLILSGCSSLKKFPLISENVEVLL---LDGTVIKSLPESIQTFRRLALLNLKNCKKLK 783

Query: 788  NIPNAIDKMKALQSLNLSGCSNLANRKRKASEAELEELDIRGIARRRRRRDDNDNDNQLV 847
            ++ + + K+K LQ L LSGCS L        + ++E L+I          DD        
Sbjct: 784  HLSSDLYKLKCLQELILSGCSQL--EVFPEIKEDMESLEI-------LLMDDTSITEMPK 843

Query: 848  GLNIFRKLFFWLCKAPAS---CIFGIPSLAGLYSLTRLNLRDCNLEVIPRGIECLVSLTE 907
             +++     F LC   +     +F +P   G   LT L L  C+L  +P  I  L SL  
Sbjct: 844  MMHLSNIKTFSLCGTSSHVSVSMFFMPPTLGCSRLTDLYLSRCSLYKLPDNIGGLSSLQS 903

Query: 908  LNLCGNNFSHLPTSISQLHNLKRLNINQCKKLVHFPELPPRILRLLSKDCVSLKSIPD-- 967
            L L GNN  +LP S +QL+NLK  ++  CK L   P LP  +  L + +C SL+++ +  
Sbjct: 904  LCLSGNNIENLPESFNQLNNLKWFDLKFCKMLKSLPVLPQNLQYLDAHECESLETLANPL 963

Query: 968  ----ISKVEHLYFLKELNLMNCYQLANNKEL---------HRLIISWMEKMLFR----KG 1027
                + +  H  F+      NCY+L  + +           +L+ +   K  +R    + 
Sbjct: 964  TPLTVGERIHSMFI----FSNCYKLNQDAQASLVGHARIKSQLMANASAKRYYRGFVPEP 1023

Query: 1028 AFNIMIPGSEIPDWFT-TKMGSSISVKWDPNSPNAHLIRFALCVLCGPIDDQDDMTDVSG 1087
               I  P +EIP WF   ++G S+ +   P+  + + +  AL V+    D +D     S 
Sbjct: 1024 LVGICYPATEIPSWFCHQRLGRSLEIPLPPHWCDINFVGLALSVVVSFKDYEDSAKRFSV 1083

Query: 1088 SIIASVTGKSHEDSNLKNGDLLVNGFT------ISGMRKL--DHIWL-----FVLPRTRS 1130
                +   K   DS+    D  + G+           RKL  DH+++     F++   ++
Sbjct: 1084 KCCGNFENK---DSSFTRFDFTLAGWNEPCGSLSHESRKLTSDHVFMGYNSCFLV---KN 1143

BLAST of Lcy12g002500 vs. TAIR 10
Match: AT5G36930.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 496.9 bits (1278), Expect = 4.3e-140
Identity = 343/963 (35.62%), Postives = 518/963 (53.79%), Query Frame = 0

Query: 12  KWKYDVFLSFRGEDTRGGFTDHLYKALVRKGIFTFRDEDEIKEGKGISSDLLAAIEASRF 71
           +W YDVF+SFRG D R  F  HLY +L R GI TF D+ E++ G+ IS +LL AIE S+ 
Sbjct: 11  RWTYDVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKI 70

Query: 72  ALVVVSQNYASSRWCLEELVKIFDC-QGRLRMSVLPVFYKVDPSDVRNQRGSFGEAFVKH 131
            +VV++++YASS WCL+ELV I    +      V P+F  VDPSD+R Q+GS+ ++F KH
Sbjct: 71  LIVVLTKDYASSAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKH 130

Query: 132 ELRFGEDDSKIRKWRQALTKLANLKAWLSQPWSHESKIIEEITEAIWKRIKPTLTVTQED 191
             +     +K++ WR+ALTK+AN+  W  +   +E++ I +IT  I KR+ P   +    
Sbjct: 131 --KNSHPLNKLKDWREALTKVANISGWDIKN-RNEAECIADITREILKRL-PCQYLHVPS 190

Query: 192 QLVGINSKLNKLFSLLNPNLVDDVVCVGIHGMGGIGKTTIARVCYERIRDEFEAHCFLSD 251
             VG+ S+L  + SLL+    D V  + I+GMGGIGKTT+A+V +      FE   FL +
Sbjct: 191 YAVGLRSRLQHISSLLSIG-SDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLEN 250

Query: 252 VRE-KFETSGLPHLQSKLLSRMFSIKNNDIWDVEEGIAMINRAIFQKKTLLVLDDVDSSD 311
            RE   +  G  HLQ +LLS +  ++ NDI + +     +      K+ LLV+DDVD   
Sbjct: 251 FREYSKKPEGRTHLQHQLLSDI--LRRNDI-EFKGLDHAVKERFRSKRVLLVVDDVDDVH 310

Query: 312 QITGLIPNKGSFGSGSRIIITTRNADLLSNEFEVKRIFEVEELQYEEALQLLNLSAFTKT 371
           Q+     ++  FG GSRIIITTRN  LL  +   +  +  +EL  +E+L+L +  AF  +
Sbjct: 311 QLNSAAIDRDCFGHGSRIIITTRNMHLL-KQLRAEGSYSPKELDGDESLELFSWHAFRTS 370

Query: 372 CPKECFLEHSKSIVKVVGGHPLALKLLGSSLRNKDLSVWNDVIEELQGGGNIHDKIFKCL 431
            P + FL+HS+ +V    G PLA+++LG+ L  + +  W   ++ L+   N  D I   L
Sbjct: 371 EPPKEFLQHSEEVVTYCAGLPLAVEVLGAFLIERSIREWESTLKLLKRIPN--DNIQAKL 430

Query: 432 KVSYDGLDEWEKEIFLDVACFFKGKRRELVEEMLNGCGFYAKRRVELLIQKSLLTLSYDN 491
           ++S++ L   +K++FLD+ACFF G     V  +L+GC  Y    + LL+++ L+T+S  N
Sbjct: 431 QISFNALTIEQKDVFLDIACFFIGVDSYYVACILDGCNLYPDIVLSLLMERCLITIS-GN 490

Query: 492 RLHMHDLLQEMGRKIVRH-KP----IQDRLWCHKDIKSVVREA----LVQSIFFKSTRIV 551
            + MHDLL++MGR+IVR   P     + RLW H D+  V+++      ++ +  K+   V
Sbjct: 491 NIMMHDLLRDMGRQIVREISPKKCGERSRLWSHNDVVGVLKKKSGTNAIEGLSLKAD--V 550

Query: 552 VEFPI----QFSKMHQLRLLNFKNVRLKSQLEYCIPTELRYLKWKGYPMEFLPLSSEEYK 611
           ++F       F+KM +LRLL  + V L    E+  P +LR+L W G+ +E  P++     
Sbjct: 551 MDFQYFEVEAFAKMQELRLLELRYVDLNGSYEH-FPKDLRWLCWHGFSLECFPINLSLES 610

Query: 612 LIGLHMCHSNLKQFWQGE---KHLEELKYIKLNHSQKLSKTPDFTKIPNLERLELEGCTS 671
           L  L + +SNLK+FW+ +   +    +KY+ L+HS  L +TPDF+  PN+E+L L  C S
Sbjct: 611 LAALDLQYSNLKRFWKAQSPPQPANMVKYLDLSHSVYLRETPDFSYFPNVEKLILINCKS 670

Query: 672 LVNIHPSI-FLAEKLIFLSLKDCINLTNLPRDI-NIKVLQVLILSGCSKVNNIPEFSGNT 731
           LV +H SI  L +KL+ L+L  CI L  LP +I  +K L+ L LS CSK+  + +  G  
Sbjct: 671 LVLVHKSIGILDKKLVLLNLSSCIELDVLPEEIYKLKSLESLFLSNCSKLERLDDALGEL 730

Query: 732 NRLLQLHLDGTSISNLPPSIATLTHLKVLSLKNCSKLINIPNAIDKMKALQSLNLSGCSN 791
             L  L  D T+                        L  IP+ I+++K L+ L+L+GC  
Sbjct: 731 ESLTTLLADFTA------------------------LREIPSTINQLKKLKRLSLNGCKG 790

Query: 792 LANRKRKASEAELEELDIRGIARRRRRRDDNDNDNQLVGLNIFRKLFFWLCKAPASCIFG 851
           L +                         DD DN               +  K+ +  +  
Sbjct: 791 LLS-------------------------DDIDN--------------LYSEKSHSVSLLR 850

Query: 852 IPSLAGLYSLTRLNLRDCNL--EVIPRGIECLVSLTELNLCGNNFSHLPTSISQLHNLKR 911
             SL+GL  +  L+L  CNL  E+IP  I  L  L +L+L GN+F +LPT  + L NL  
Sbjct: 851 PVSLSGLTYMRILSLGYCNLSDELIPEDIGSLSFLRDLDLRGNSFCNLPTDFATLPNLGE 892

Query: 912 LNINQCKKLVHFPELPPRILRLLSKDCVSLKSIPDISKVEHLYFLKELNLMNCYQLANNK 953
           L ++ C KL     LP  +L L    C+ LK  PDISK   L+   +L L +C  L    
Sbjct: 911 LLLSDCSKLQSILSLPRSLLFLDVGKCIMLKRTPDISKCSALF---KLQLNDCISLFEIP 892

BLAST of Lcy12g002500 vs. TAIR 10
Match: AT5G36930.2 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 496.9 bits (1278), Expect = 4.3e-140
Identity = 343/963 (35.62%), Postives = 518/963 (53.79%), Query Frame = 0

Query: 12  KWKYDVFLSFRGEDTRGGFTDHLYKALVRKGIFTFRDEDEIKEGKGISSDLLAAIEASRF 71
           +W YDVF+SFRG D R  F  HLY +L R GI TF D+ E++ G+ IS +LL AIE S+ 
Sbjct: 14  RWTYDVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKI 73

Query: 72  ALVVVSQNYASSRWCLEELVKIFDC-QGRLRMSVLPVFYKVDPSDVRNQRGSFGEAFVKH 131
            +VV++++YASS WCL+ELV I    +      V P+F  VDPSD+R Q+GS+ ++F KH
Sbjct: 74  LIVVLTKDYASSAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKH 133

Query: 132 ELRFGEDDSKIRKWRQALTKLANLKAWLSQPWSHESKIIEEITEAIWKRIKPTLTVTQED 191
             +     +K++ WR+ALTK+AN+  W  +   +E++ I +IT  I KR+ P   +    
Sbjct: 134 --KNSHPLNKLKDWREALTKVANISGWDIKN-RNEAECIADITREILKRL-PCQYLHVPS 193

Query: 192 QLVGINSKLNKLFSLLNPNLVDDVVCVGIHGMGGIGKTTIARVCYERIRDEFEAHCFLSD 251
             VG+ S+L  + SLL+    D V  + I+GMGGIGKTT+A+V +      FE   FL +
Sbjct: 194 YAVGLRSRLQHISSLLSIG-SDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLEN 253

Query: 252 VRE-KFETSGLPHLQSKLLSRMFSIKNNDIWDVEEGIAMINRAIFQKKTLLVLDDVDSSD 311
            RE   +  G  HLQ +LLS +  ++ NDI + +     +      K+ LLV+DDVD   
Sbjct: 254 FREYSKKPEGRTHLQHQLLSDI--LRRNDI-EFKGLDHAVKERFRSKRVLLVVDDVDDVH 313

Query: 312 QITGLIPNKGSFGSGSRIIITTRNADLLSNEFEVKRIFEVEELQYEEALQLLNLSAFTKT 371
           Q+     ++  FG GSRIIITTRN  LL  +   +  +  +EL  +E+L+L +  AF  +
Sbjct: 314 QLNSAAIDRDCFGHGSRIIITTRNMHLL-KQLRAEGSYSPKELDGDESLELFSWHAFRTS 373

Query: 372 CPKECFLEHSKSIVKVVGGHPLALKLLGSSLRNKDLSVWNDVIEELQGGGNIHDKIFKCL 431
            P + FL+HS+ +V    G PLA+++LG+ L  + +  W   ++ L+   N  D I   L
Sbjct: 374 EPPKEFLQHSEEVVTYCAGLPLAVEVLGAFLIERSIREWESTLKLLKRIPN--DNIQAKL 433

Query: 432 KVSYDGLDEWEKEIFLDVACFFKGKRRELVEEMLNGCGFYAKRRVELLIQKSLLTLSYDN 491
           ++S++ L   +K++FLD+ACFF G     V  +L+GC  Y    + LL+++ L+T+S  N
Sbjct: 434 QISFNALTIEQKDVFLDIACFFIGVDSYYVACILDGCNLYPDIVLSLLMERCLITIS-GN 493

Query: 492 RLHMHDLLQEMGRKIVRH-KP----IQDRLWCHKDIKSVVREA----LVQSIFFKSTRIV 551
            + MHDLL++MGR+IVR   P     + RLW H D+  V+++      ++ +  K+   V
Sbjct: 494 NIMMHDLLRDMGRQIVREISPKKCGERSRLWSHNDVVGVLKKKSGTNAIEGLSLKAD--V 553

Query: 552 VEFPI----QFSKMHQLRLLNFKNVRLKSQLEYCIPTELRYLKWKGYPMEFLPLSSEEYK 611
           ++F       F+KM +LRLL  + V L    E+  P +LR+L W G+ +E  P++     
Sbjct: 554 MDFQYFEVEAFAKMQELRLLELRYVDLNGSYEH-FPKDLRWLCWHGFSLECFPINLSLES 613

Query: 612 LIGLHMCHSNLKQFWQGE---KHLEELKYIKLNHSQKLSKTPDFTKIPNLERLELEGCTS 671
           L  L + +SNLK+FW+ +   +    +KY+ L+HS  L +TPDF+  PN+E+L L  C S
Sbjct: 614 LAALDLQYSNLKRFWKAQSPPQPANMVKYLDLSHSVYLRETPDFSYFPNVEKLILINCKS 673

Query: 672 LVNIHPSI-FLAEKLIFLSLKDCINLTNLPRDI-NIKVLQVLILSGCSKVNNIPEFSGNT 731
           LV +H SI  L +KL+ L+L  CI L  LP +I  +K L+ L LS CSK+  + +  G  
Sbjct: 674 LVLVHKSIGILDKKLVLLNLSSCIELDVLPEEIYKLKSLESLFLSNCSKLERLDDALGEL 733

Query: 732 NRLLQLHLDGTSISNLPPSIATLTHLKVLSLKNCSKLINIPNAIDKMKALQSLNLSGCSN 791
             L  L  D T+                        L  IP+ I+++K L+ L+L+GC  
Sbjct: 734 ESLTTLLADFTA------------------------LREIPSTINQLKKLKRLSLNGCKG 793

Query: 792 LANRKRKASEAELEELDIRGIARRRRRRDDNDNDNQLVGLNIFRKLFFWLCKAPASCIFG 851
           L +                         DD DN               +  K+ +  +  
Sbjct: 794 LLS-------------------------DDIDN--------------LYSEKSHSVSLLR 853

Query: 852 IPSLAGLYSLTRLNLRDCNL--EVIPRGIECLVSLTELNLCGNNFSHLPTSISQLHNLKR 911
             SL+GL  +  L+L  CNL  E+IP  I  L  L +L+L GN+F +LPT  + L NL  
Sbjct: 854 PVSLSGLTYMRILSLGYCNLSDELIPEDIGSLSFLRDLDLRGNSFCNLPTDFATLPNLGE 895

Query: 912 LNINQCKKLVHFPELPPRILRLLSKDCVSLKSIPDISKVEHLYFLKELNLMNCYQLANNK 953
           L ++ C KL     LP  +L L    C+ LK  PDISK   L+   +L L +C  L    
Sbjct: 914 LLLSDCSKLQSILSLPRSLLFLDVGKCIMLKRTPDISKCSALF---KLQLNDCISLFEIP 895

BLAST of Lcy12g002500 vs. TAIR 10
Match: AT4G19510.1 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 476.5 bits (1225), Expect = 6.1e-134
Identity = 347/1049 (33.08%), Postives = 522/1049 (49.76%), Query Frame = 0

Query: 4    SSSCSSSGKWKYDVFLSFRGEDTRGGFTDHLYKALVRKGIFTFRDEDEIKEGKGISSDLL 63
            SSS     K ++DVF+SFRG DTR  FT HL K L  KGI  F D  +++ G+ IS  L 
Sbjct: 46   SSSSIVLSKCEFDVFVSFRGADTRHDFTSHLVKYLRGKGIDVFSDA-KLRGGEYISL-LF 105

Query: 64   AAIEASRFALVVVSQNYASSRWCLEELVKIFDCQGRLRMSVLPVFYKVDPSDVRNQRGSF 123
              IE S+ ++VV S++YA+S WCLEE+ KI   +      VLP+FYKV  SDV NQ GSF
Sbjct: 106  DRIEQSKMSIVVFSEDYANSWWCLEEVGKIMQRRKEFNHGVLPIFYKVSKSDVSNQTGSF 165

Query: 124  GEAFVKHELRFGEDDSKIRKWRQALTKLANLKAWLSQPWSHESKIIEEITEAIWKRIKPT 183
               F      F  D+ KI + + AL   +N++ ++    S E   ++EI +  ++ +   
Sbjct: 166  EAVFQSPTKIFNGDEQKIEELKVALKTASNIRGFVYPENSSEPDFLDEIVKNTFRMLNEL 225

Query: 184  LTVTQEDQLVGINSKLNKLFSLLNPNLVDDVVCVGIHGMGGIGKTTIARVCYERIRDEFE 243
                  D L GI S+  +L  LL  +  + V  VG+ GM GIGKTT+A + Y++    F+
Sbjct: 226  SPCVIPDDLPGIESRSKELEKLLMFDNDECVRVVGVLGMTGIGKTTVADIVYKQNFQRFD 285

Query: 244  AHCFLSDVREKFETSGLPHLQSKLLSRMFSIKNNDIWDVEEGIAMINRAIFQKKTLLVLD 303
             + FL D+ +  +  GLP+L  KLL ++   +N D+             +  KK  +VLD
Sbjct: 286  GYEFLEDIEDNSKRYGLPYLYQKLLHKLLDGENVDV----RAQGRPENFLRNKKLFIVLD 345

Query: 304  DVDSSDQITGLIPNKGSFGSGSRIIITTRNADLLSNEFEVKRIFEVEELQYEEALQLLNL 363
            +V    QI  LI  K  +  GSRI+I TR+  LL    +    + V  L   EA++L  L
Sbjct: 346  NVTEEKQIEYLIGKKNVYRQGSRIVIITRDKKLLQKNADA--TYVVPRLNDREAMELFCL 405

Query: 364  SAFTKTCPKECFLEHSKSIVKVVGGHPLALKLLGSSLRNKDLSVWNDVIEELQGGGNIHD 423
              F    P E F++ S   V    G PLALKLLG  L   D++ W   +E LQ   N   
Sbjct: 406  QVFGNHYPTEEFVDLSNDFVCYAKGLPLALKLLGKGLLTHDINYWKKKLEFLQ--VNPDK 465

Query: 424  KIFKCLKVSYDGLDEWEKEIFLDVACFFKGKRRELVEEMLNGCGFYAKRRVELLIQKSLL 483
            ++ K LK SY  LD+ +K +FLD+ACFF                                
Sbjct: 466  ELQKELKSSYKALDDDQKSVFLDIACFF-------------------------------- 525

Query: 484  TLSYDNRLHMHDLLQEMGRKIVRHKPIQD-----RLWCHKDIKSVVRE----ALVQSIFF 543
                  R+ MHDLL  MG++I + K I+      RLW HKDI+ ++        V+ IF 
Sbjct: 526  ------RIEMHDLLHAMGKEIGKEKSIRKAGERRRLWNHKDIRDILEHNTGTECVRGIFL 585

Query: 544  KSTRI--VVEFPIQFSKMHQLRLLNFKNVRLK-----------SQLEYCIPTELRYLKWK 603
              + +  +  FP  F+ + +L+ L F +               S++    P EL YL W+
Sbjct: 586  NMSEVRRIKLFPAAFTMLSKLKFLKFHSSHCSQWCDNDHIFQCSKVPDHFPDELVYLHWQ 645

Query: 604  GYPMEFLPLSSEEYKLIGLHMCHSNLKQFWQGEKHLEELKYIKLNHSQKLSKTPDFTKIP 663
            GYP + LP   +  +L+ L + +S++KQ W+ EK+ E L+++ L  S+ L      ++  
Sbjct: 646  GYPYDCLPSDFDPKELVDLSLRYSHIKQLWEDEKNTESLRWVDLGQSKDLLNLSGLSRAK 705

Query: 664  NLERLELEGCTSLVNIHPSIFLAEKLIFLSLKDCINLTNLPRDINIKVLQVLILSGCSKV 723
            NLERL+LEGCTSL ++  S+    +LI+L+L+DC +L +LP+   IK L+ LILSGC K 
Sbjct: 706  NLERLDLEGCTSL-DLLGSVKQMNELIYLNLRDCTSLESLPKGFKIKSLKTLILSGCLK- 765

Query: 724  NNIPEFSGNTNRLLQLHLDGTSISNLPPSIATLTHLKVLSLKNCSKLINIPNAIDKMKAL 783
              + +F   +  +  LHL+GT+I  +   I +L  L +L+LKNC KL  +PN + K+K+L
Sbjct: 766  --LKDFHIISESIESLHLEGTAIERVVEHIESLHSLILLNLKNCEKLKYLPNDLYKLKSL 825

Query: 784  QSLNLSGCSNLANRKRKASEAELEELDIRGIARRRRRRDDNDNDNQLVGLNIFRKL-FFW 843
            Q L LSGCS L +      + E  E+ +           D  +  Q   ++    L    
Sbjct: 826  QELVLSGCSALESLPPIKEKMECLEILLM----------DGTSIKQTPEMSCLSNLKICS 885

Query: 844  LCKAPASCIFGIPSL--AGLYSLTRLNLRDCNLEVIPRGIECLVSLTELNLCGNNFSHLP 903
             C+       G+  L  +G   L+ L L +CN++ +P     L SL  L L  NN   LP
Sbjct: 886  FCRPVIDDSTGLVVLPFSGNSFLSDLYLTNCNIDKLPDKFSSLRSLRCLCLSRNNIETLP 945

Query: 904  TSISQLHNLKRLNINQCKKLVHFPELPPRILRLLSKDCVSLK------SIPDISKVEHLY 963
             SI +L++L  L++  C +L   P LP  +  L +  C SL+      +IP +++  H  
Sbjct: 946  ESIEKLYSLLLLDLKHCCRLKSLPLLPSNLQYLDAHGCGSLENVSKPLTIPLVTERMHTT 1005

Query: 964  FL----KELNLMNCYQLANNKELHRLIISWMEKMLFRKGAF-----NIMIPGSEIPDWFT 1012
            F+     +LN      +    +L   +++   +    KG        +  PG +IP WF+
Sbjct: 1006 FIFTDCFKLNQAEKEDIVAQAQLKSQLLARTSRHHNHKGLLLDPLVAVCFPGHDIPSWFS 1032