Homology
BLAST of Lcy11g016530 vs. ExPASy Swiss-Prot
Match:
Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)
HSP 1 Score: 832.4 bits (2149), Expect = 4.2e-240
Identity = 424/767 (55.28%), Postives = 545/767 (71.06%), Query Frame = 0
Query: 12 LLLLFFFLISLLQTSTIATEKSYIVYLGSQLSSSLGPSALSEQSRQVTASHYDLLGSMLG 71
LLLL +S SY+VY G+ S +G R V +HYD LGS G
Sbjct: 10 LLLLLLVHMSSKHILASKDSSSYVVYFGAH--SHVGEITEDAMDR-VKETHYDFLGSFTG 69
Query: 72 SKTMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPKVISVFENKPRKLHTTRSWNFLGL 131
S+ A +AIFYSYT+ NGFAA LD A +++++P+V+SVF NK KLHTTRSW+FLGL
Sbjct: 70 SRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGL 129
Query: 132 ENDAGIPSNSIWNAAKFGQDVILANVDTGVWPESQSFSDEGYGPVPSKWRGICQS--DST 191
E+++ +PS+SIW A+FG+D I+AN+DTGVWPES+SF DEG GP+PS+W+GICQ+ D+T
Sbjct: 130 EHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDAT 189
Query: 192 FHCNKKLIGARYFYKGYEAAGGVLNASLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGN 251
FHCN+KLIGARYF KGY AA G LN+S + RD DGHG+HTLSTAAG+FV G ++FG GN
Sbjct: 190 FHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGN 249
Query: 252 GTAKGGAPKARVAAYKVCWPSFMDGQCFDADTLAAFEAAIADGVDVISVSLGGTANEFVN 311
GTAKGG+P+ARVAAYKVCWP +C+DAD LAAF+AAI DG DVISVSLGG F N
Sbjct: 250 GTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFN 309
Query: 312 DPLSIVAFHAVQQGIVVVFSAGNSGPFPGTVTNVAPWQITVAASTDDRDFASYVALGNKK 371
D ++I +FHA ++ IVVV SAGNSGP TV+NVAPWQITV AST DR+FAS + LGN K
Sbjct: 310 DSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGK 369
Query: 372 HLKGVSLSSVAPLPKKFYPLITSVDARFSNVTEYHAQFCDEGTLDPIKVKGKILICLSGE 431
H KG SLSS A KFYP++ SV+A+ N + AQ C G+LDPIK KGKIL+CL G+
Sbjct: 370 HYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQ 429
Query: 432 IEGIDKGFQAARAGAVGVVVANDLEVGNEIYPELHFLPASDITSNDNQLLQQYLNSTKTP 491
++KG A G +G+V+ N GN++ + H LPA+ +TS D+ + +Y++ TK P
Sbjct: 430 NGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKP 489
Query: 492 VAHLKKVITQLGVKPAPVMASFSSRGPNPIESSILKPDITAPGVNILASYPTGKAPTSSI 551
+AH+ T LG+KPAPVMASFSS+GP+ + ILKPDITAPGV+++A+Y +PT+
Sbjct: 490 IAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQ 549
Query: 552 SDRRRIPFNVISGTSMSCPHISGIAALLKSIHPEWSPAAIKSAIVTTAKTRGNNLQTILD 611
D RR+ FN ISGTSMSCPHISGIA LLK+ +P WSPAAI+SAI+TTA + I +
Sbjct: 550 FDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQN 609
Query: 612 SNKLRASPFAYGAGLVNPNNAMDPGLVYDTTVDDYLNFLCARGYNATQIRQFDTKPFICS 671
+ ++A+PF++GAG V PN A++PGLVYD + DYLNFLC+ GYNA+QI F F CS
Sbjct: 610 ATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCS 669
Query: 672 K-SFKLTDLNYPSISVGELKIGAPVTINRRVKNVGNPGTYVARVKVSPGVAVSVEPSTLQ 731
L +LNYPSI+V L + VT++R VKNVG P Y +V GV V+V+P++L
Sbjct: 670 SPKISLVNLNYPSITVPNL-TSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLN 729
Query: 732 FSSVGEEKAFKVVLQNTGKVTRNGYVFGTLIWSDGKHFVRSPMAIDL 776
F+ VGE+K FKV+L + GYVFG L+WSD KH VRSP+ + L
Sbjct: 730 FTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772
BLAST of Lcy11g016530 vs. ExPASy Swiss-Prot
Match:
F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)
HSP 1 Score: 813.5 bits (2100), Expect = 2.0e-234
Identity = 429/774 (55.43%), Postives = 529/774 (68.35%), Query Frame = 0
Query: 5 SNNKLPPLLLLFFFLISLLQTSTIATEKSYIVYLGSQLSSSLGPSALSEQSRQVTASHYD 64
SN L L++L + A +KSYIVYLGS P S V SH
Sbjct: 13 SNKMSLQSLSSLLLLVTLFFSPAFALKKSYIVYLGSHAHL---PQISSAHLDGVAHSHRT 72
Query: 65 LLGSMLGSKTMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPKVISVFENKPRKLHTTR 124
L S +GS A+EAIFYSY R NGFAA LDE EAA++A++P V+SVF NK RKLHTT
Sbjct: 73 FLASFVGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTH 132
Query: 125 SWNFLGLENDAGIPSNSIWNAAKFGQDVILANVDTGVWPESQSFSDEGYGPVPSKWRGIC 184
SWNF+ L + + +S+WN A +G+D I+AN+DTGVWPES+SFSDEGYG VP++W+G C
Sbjct: 133 SWNFMLLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRC 192
Query: 185 QSDSTFHCNKKLIGARYFYKGYEAAGGV-LNASLLTVRDHDGHGTHTLSTAAGNFVTGAN 244
D CN+KLIGARYF KGY A G+ NAS T RDHDGHG+HTLSTAAGNFV GAN
Sbjct: 193 HKD--VPCNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGAN 252
Query: 245 VFGHGNGTAKGGAPKARVAAYKVCWPSFMDGQCFDADTLAAFEAAIADGVDVISVSLGGT 304
VFG GNGTA GG+PKARVAAYKVCWP +CFDAD LAA EAAI DGVDV+S S+GG
Sbjct: 253 VFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGD 312
Query: 305 ANEFVNDPLSIVAFHAVQQGIVVVFSAGNSGPFPGTVTNVAPWQITVAASTDDRDFASYV 364
A ++++D ++I +FHAV+ G+ VV SAGNSGP GTV+NVAPW ITV AS+ DR+F ++V
Sbjct: 313 AGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFV 372
Query: 365 ALGNKKHLKGVSLSSVAPLP-KKFYPLITSVDARFSNVTEYHAQFCDEGTLDPIKVKGKI 424
L N + KG SLS PLP +K Y LI++ DA +N A C +G+LDP KVKGKI
Sbjct: 373 ELKNGQSFKGTSLSK--PLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKI 432
Query: 425 LICLSGEIEGIDKGFQAARAGAVGVVVANDLEVGNEIYPELHFLPASDITSNDNQLLQQY 484
L+CL G+ +DKG QAA AGA G+V+ ND GNEI + H LPAS I D + L Y
Sbjct: 433 LVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSY 492
Query: 485 LNSTKTPVAHLKKVITQLGVKPAPVMASFSSRGPNPIESSILKPDITAPGVNILASYPTG 544
L+STK P ++K L KPAP MASFSSRGPN I ILKPDITAPGVNI+A++
Sbjct: 493 LSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEA 552
Query: 545 KAPTSSISDRRRIPFNVISGTSMSCPHISGIAALLKSIHPEWSPAAIKSAIVTTAKTRGN 604
PT SD RR PFN SGTSMSCPHISG+ LLK++HP WSPAAI+SAI+TT++TR N
Sbjct: 553 TGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNN 612
Query: 605 NLQTILDSNKLRASPFAYGAGLVNPNNAMDPGLVYDTTVDDYLNFLCARGYNATQIRQFD 664
+ ++D + +A+PF+YG+G V PN A PGLVYD T DYL+FLCA GYN T ++ F
Sbjct: 613 RRKPMVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFA 672
Query: 665 TKP-FICSKSFKLTDLNYPSISVGELKIGAPVTINRRVKNVGNPGTYVARVKVSPGVAVS 724
P + C + L D NYPSI+V L +T+ R++KNVG P TY AR + GV VS
Sbjct: 673 EDPQYTCRQGANLLDFNYPSITVPNLT--GSITVTRKLKNVGPPATYNARFREPLGVRVS 732
Query: 725 VEPSTLQFSSVGEEKAFKVVLQNTGKVTRNGYVFGTLIWSDGKHFVRSPMAIDL 776
VEP L F+ GE K F++ L+ VT +GYVFG L W+D H+VRSP+ + L
Sbjct: 733 VEPKQLTFNKTGEVKIFQMTLRPL-PVTPSGYVFGELTWTDSHHYVRSPIVVQL 776
BLAST of Lcy11g016530 vs. ExPASy Swiss-Prot
Match:
I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)
HSP 1 Score: 724.5 bits (1869), Expect = 1.2e-207
Identity = 402/786 (51.15%), Postives = 508/786 (64.63%), Query Frame = 0
Query: 12 LLLLFFFLISLLQTSTIATEKSYIVYLGSQLSSSLGPSALSEQSRQVTASHYDLLGSMLG 71
L+L FFL + L + ++K YIVY+G+ S GPS S T SHYDLLGS+ G
Sbjct: 10 LILSSFFLFTFLLAAVNGSKKCYIVYMGAH---SHGPSPTSADLELATDSHYDLLGSIFG 69
Query: 72 SKTMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPKVISVFENKPRKLHTTRSWNFLGL 131
S+ A+EAI YSY R NGFAA L+E+EAAD+A+NP V+SVF +K KLHTTRSW FLGL
Sbjct: 70 SREKAKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGL 129
Query: 132 ENDAGIPSNSIWNAAKFGQDVILANVDTGVWPESQSFSDEGYGPVPSKWR-GICQ----- 191
NS W +FG++ I+ N+DTGVWPESQSFSD+GYG VPSKWR G+CQ
Sbjct: 130 HRRG---QNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKLP 189
Query: 192 SDSTFHCNKKLIGARYFYKGYEAAGGVLNASLLTVRDHDGHGTHTLSTAAGNFVTGANVF 251
CN+KLIGARY+ K +EA G L+ L T RD GHGTHTLSTA GNFV GA VF
Sbjct: 190 GSMKNTCNRKLIGARYYNKAFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVF 249
Query: 252 GHGNGTAKGGAPKARVAAYKVCWPSFMDGQCFDADTLAAFEAAIADGVDVISVSLG---- 311
GNGTAKGG+P+ARVAAYKVCW C+ AD LAA + AI DGVDVI+VS G
Sbjct: 250 AVGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYV 309
Query: 312 GTANEFVNDPLSIVAFHAVQQGIVVVFSAGNSGPFPGTVTNVAPWQITVAASTDDRDFAS 371
TA D +SI AFHA+ + I++V SAGN GP PGTV NVAPW T+AAST DRDF+S
Sbjct: 310 VTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSS 369
Query: 372 YVALGNKKHLKGVSLSSVAPLPKKFYPLITSVDARFSNVTEYHAQFCDEGTLDPIKVKGK 431
+ + N+ ++G SL P P + + LI S DA+ +N T AQ C GTLD KV GK
Sbjct: 370 NLTINNQL-IEGASLFVNLP-PNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGK 429
Query: 432 ILICL-SGEIEGIDKGFQAARAGAVGVVVANDLEVGNEIYPELHFL-----PASDITSND 491
I++C G+I+ + +G +A AGA G+++ N ++ G + E H P S
Sbjct: 430 IVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRP 489
Query: 492 NQLLQQYLNSTKTPV-----AHLKKVITQLGVKPAPVMASFSSRGPNPIESSILKPDITA 551
+ + + P+ + + T G KPAPVMASFSSRGPN I+ SILKPD+TA
Sbjct: 490 HGVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTA 549
Query: 552 PGVNILASYPTGKAPTSSISDRRR-IPFNVISGTSMSCPHISGIAALLKSIHPEWSPAAI 611
PGVNILA+Y + +S + D RR FNV+ GTSMSCPH SGIA LLK+ HP WSPAAI
Sbjct: 550 PGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAI 609
Query: 612 KSAIVTTAKTRGNNLQTILDS-NKLRASPFAYGAGLVNPNNAMDPGLVYDTTVDDYLNFL 671
KSAI+TTA T N + I D+ +K A FAYG+G V P+ A++PGLVYD ++ DYLNFL
Sbjct: 610 KSAIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFL 669
Query: 672 CARGYNATQIRQFD-TKPFICSKSFKLTDLNYPSISVGELKIGAPVTINRRVKNVGNPGT 731
CA GY+ I + + FICS S + DLNYPSI++ L++ PVTI R V NVG P T
Sbjct: 670 CASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRL-KPVTIARTVTNVGPPST 729
Query: 732 YVARVKVSPGVAVSVEPSTLQFSSVGEEKAFKVVLQNTGKVTRNGYVFGTLIWSDGKHFV 774
Y + G +++V P +L F+ +GE K FKV++Q + TR Y FG L W+DGKH V
Sbjct: 730 YTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIV 786
BLAST of Lcy11g016530 vs. ExPASy Swiss-Prot
Match:
O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)
HSP 1 Score: 601.3 bits (1549), Expect = 1.6e-170
Identity = 344/771 (44.62%), Postives = 481/771 (62.39%), Query Frame = 0
Query: 16 FFFLISL---LQTSTIATEKSYIVYLG-SQLSSSLGPSALSEQSRQVTASHYDLLGSMLG 75
FF L+ L +S+ + + +YIV++ SQ+ SS + ++ YD S L
Sbjct: 11 FFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSF----------DLHSNWYD---SSLR 70
Query: 76 SKTMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPKVISVFENKPRKLHTTRSWNFLGL 135
S + + E + Y+Y + +GF+ +L ++EA L P VISV +LHTTR+ FLGL
Sbjct: 71 SISDSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGL 130
Query: 136 ENDAGIPSNSIWNAAKFGQDVILANVDTGVWPESQSFSDEGYGPVPSKWRGICQSDSTFH 195
+ ++ A DV++ +DTGVWPES+S+SDEG+GP+PS W+G C++ + F
Sbjct: 131 DEHTA----DLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFT 190
Query: 196 ---CNKKLIGARYFYKGYEAAGGVLNAS--LLTVRDHDGHGTHTLSTAAGNFVTGANVFG 255
CN+KLIGAR+F +GYE+ G ++ S + RD DGHGTHT STAAG+ V GA++ G
Sbjct: 191 ASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLG 250
Query: 256 HGNGTAKGGAPKARVAAYKVCWPSFMDGQCFDADTLAAFEAAIADGVDVISVSLGGTANE 315
+ +GTA+G AP+ARVA YKVCW G CF +D LAA + AIAD V+V+S+SLGG ++
Sbjct: 251 YASGTARGMAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSD 310
Query: 316 FVNDPLSIVAFHAVQQGIVVVFSAGNSGPFPGTVTNVAPWQITVAASTDDRDFASYVALG 375
+ D ++I AF A+++GI+V SAGN+GP +++NVAPW TV A T DRDF + LG
Sbjct: 311 YYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILG 370
Query: 376 NKKHLKGVSLSSVAPLPKKFYPLITSVDARFSNVTEYHAQFCDEGTLDPIKVKGKILICL 435
N K+ GVSL LP K P I + +A SN T + C GTL P KVKGKI++C
Sbjct: 371 NGKNFTGVSLFKGEALPDKLLPFIYAGNA--SNAT--NGNLCMTGTLIPEKVKGKIVMCD 430
Query: 436 SGEIEGIDKGFQAARAGAVGVVVANDLEVGNEIYPELHFLPASDITSNDNQLLQQYLNST 495
G + KG AG VG+++AN G E+ + H LPA+ + +++ Y+ +
Sbjct: 431 RGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTD 490
Query: 496 KTPVAHLKKVITQLGVKPAPVMASFSSRGPNPIESSILKPDITAPGVNILASYPTGKAPT 555
P A + + T +GVKP+PV+A+FSSRGPN I +ILKPD+ APGVNILA++ PT
Sbjct: 491 PNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPT 550
Query: 556 SSISDRRRIPFNVISGTSMSCPHISGIAALLKSIHPEWSPAAIKSAIVTTAKTRGNNLQT 615
SD RR+ FN+ISGTSMSCPH+SG+AALLKS+HPEWSPAAI+SA++TTA + +
Sbjct: 551 GLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKP 610
Query: 616 ILD-SNKLRASPFAYGAGLVNPNNAMDPGLVYDTTVDDYLNFLCARGYNATQIRQFDTKP 675
+LD + ++PF +GAG V+P A +PGL+YD T +DYL FLCA Y + QIR +
Sbjct: 611 LLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRN 670
Query: 676 FIC--SKSFKLTDLNYPSISVGELKIGAPVTINRRVKNVGNPGTYVARV-KVSPGVAVSV 735
+ C SKS+ + DLNYPS +V +GA R V +VG GTY +V + GV +SV
Sbjct: 671 YTCDPSKSYSVADLNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTYSVKVTSETTGVKISV 730
Query: 736 EPSTLQFSSVGEEKAFKVVLQNTGKVTRNGYVFGTLIWSDGKHFVRSPMAI 774
EP+ L F E+K++ V FG++ WSDGKH V SP+AI
Sbjct: 731 EPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754
BLAST of Lcy11g016530 vs. ExPASy Swiss-Prot
Match:
O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)
HSP 1 Score: 575.5 bits (1482), Expect = 9.2e-163
Identity = 323/723 (44.67%), Postives = 452/723 (62.52%), Query Frame = 0
Query: 72 SKTMAQEA-IFYSYTRSFNGFAAKLDEKEAADLARNPKVISVFENKPRKLHTTRSWNFLG 131
S A+E+ I + Y F+GF+A + EA +L +P V++VFE++ R+LHTTRS FLG
Sbjct: 49 STEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLG 108
Query: 132 LENDAGIPSNSIWNAAKFGQDVILANVDTGVWPESQSFSDEGYGPVPSKWRGICQSDSTF 191
L+N G +W+ + +G DVI+ DTG+WPE +SFSD GP+P +WRG+C+S + F
Sbjct: 109 LQNQKG-----LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARF 168
Query: 192 ---HCNKKLIGARYFYKGYEAA--GGV-LNASLLTVRDHDGHGTHTLSTAAGNFVTGANV 251
+CN+K+IGAR+F KG +AA GG+ L+ RD DGHGTHT STAAG A++
Sbjct: 169 SPRNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASM 228
Query: 252 FGHGNGTAKGGAPKARVAAYKVCWPSFMDGQCFDADTLAAFEAAIADGVDVISVSLG--- 311
G+ +G AKG APKAR+AAYKVCW D C D+D LAAF+AA+ DGVDVIS+S+G
Sbjct: 229 SGYASGVAKGVAPKARIAAYKVCW---KDSGCLDSDILAAFDAAVRDGVDVISISIGGGD 288
Query: 312 GTANEFVNDPLSIVAFHAVQQGIVVVFSAGNSGPFPGTVTNVAPWQITVAASTDDRDFAS 371
G + + DP++I ++ A +GI V SAGN GP +VTN+APW TV AST DR+F +
Sbjct: 289 GITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPA 348
Query: 372 YVALGNKKHLKGVSLSSVAPLPKKFYPLITSVDARFSNVTEYHAQFCDEGTLDPIKVKGK 431
LG+ L+GVSL + PL + +P++ + S+ A C E TLDP +V+GK
Sbjct: 349 DAILGDGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSS-----ASLCMENTLDPKQVRGK 408
Query: 432 ILICLSGEIEGIDKGFQAARAGAVGVVVANDLEVGNEIYPELHFLPASDITSNDNQLLQQ 491
I+IC G + KG +AG VG+++AN G + + H +PA + SN+ ++
Sbjct: 409 IVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKA 468
Query: 492 YLNSTKTPVAHLKKVITQLGVKPAPVMASFSSRGPNPIESSILKPDITAPGVNILASYPT 551
Y +S P+A + T +G+KPAPV+ASFS RGPN + ILKPD+ APGVNILA++
Sbjct: 469 YASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTD 528
Query: 552 GKAPTSSISDRRRIPFNVISGTSMSCPHISGIAALLKSIHPEWSPAAIKSAIVTTAKTRG 611
PT SD R+ FN++SGTSM+CPH+SG AALLKS HP+WSPA I+SA++TT
Sbjct: 529 AVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVD 588
Query: 612 NNLQTILD-SNKLRASPFAYGAGLVNPNNAMDPGLVYDTTVDDYLNFLCARGYNATQIRQ 671
N+ ++++D S A+P+ YG+G +N AM+PGLVYD T DDY+ FLC+ GY I+
Sbjct: 589 NSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQV 648
Query: 672 FDTKPFICSKSFKLT--DLNYPSISV---GELKIGAPVTINRRVKNVGN-PGTYVARVKV 731
P C + K + +LNYPSI+ + T+ R NVG Y AR++
Sbjct: 649 ITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIES 708
Query: 732 SPGVAVSVEPSTLQFSSVGEEKAFKV-VLQNTGKVT--RNGYVFGTLIWSD-GKHFVRSP 774
GV V+V+P L F+S + +++ V V NT V G VFG++ W D GKH VRSP
Sbjct: 709 PRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSP 758
BLAST of Lcy11g016530 vs. ExPASy TrEMBL
Match:
A0A1S4DX90 (subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491001 PE=3 SV=1)
HSP 1 Score: 1231.9 bits (3186), Expect = 0.0e+00
Identity = 600/743 (80.75%), Postives = 672/743 (90.44%), Query Frame = 0
Query: 33 SYIVYLGSQLSSSLGPSALSEQSRQVTASHYDLLGSMLGSKTMAQEAIFYSYTRSFNGFA 92
SYIVYLGS+ SSSL PS+LSE S+QVTA HYDLLGS LGSKTMA+EAIFYSYTRSFNGFA
Sbjct: 106 SYIVYLGSEHSSSLDPSSLSEHSQQVTALHYDLLGSSLGSKTMAEEAIFYSYTRSFNGFA 165
Query: 93 AKLDEKEAADLARNPKVISVFENKPRKLHTTRSWNFLGLENDAGIPSNSIWNAAKFGQDV 152
A LD+KEA +LARNPKVISVFENK RKLHTTRSWNFLG+END GIPSNSIWNAAKFG+DV
Sbjct: 166 AILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPSNSIWNAAKFGEDV 225
Query: 153 ILANVDTGVWPESQSFSDEGYGPVPSKWRGICQSDSTFHCNKKLIGARYFYKGYEAAGGV 212
I+AN+DTGVWPES+SFSDEGYGPVPSKWRGICQ+DS FHCN+KLIG RYF+KGYEAAGG
Sbjct: 226 IIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNRKLIGGRYFHKGYEAAGGK 285
Query: 213 LNASLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARVAAYKVCWPSFM 272
LNA+LLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKAR AYKVCWP
Sbjct: 286 LNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARAVAYKVCWPPLF 345
Query: 273 DGQCFDADTLAAFEAAIADGVDVISVSLGGTANEFVNDPLSIVAFHAVQQGIVVVFSAGN 332
D QCFDAD LAAFEAAIADGVDV+S SLGG A+E+ NDPL+I AF AVQ+GI+VVFS GN
Sbjct: 346 DSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLAVQRGILVVFSGGN 405
Query: 333 SGPFPGTVTNVAPWQITVAASTDDRDFASYVALGNKKHLKGVSLSSVAPLPKKFYPLITS 392
SGPFP TVTNV+PW TVAA+T DRDF SYV LGNKKH+KGVSLSSVA LPKKFYPLI S
Sbjct: 406 SGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGVSLSSVASLPKKFYPLINS 465
Query: 393 VDARFSNVTEYHAQFCDEGTLDPIKVKGKILICLSGEIEGIDKGFQAARAGAVGVVVAND 452
VDA+FSNVTE+HAQFC +GTLDP+KVKGKI+IC GE EG+DKG+QA+ AGA GV+VAND
Sbjct: 466 VDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVDKGYQASLAGAAGVIVAND 525
Query: 453 LEVGNEIYPELHFLPASDITSNDNQLLQQYLNSTKTPVAHLKKVITQLGVKPAPVMASFS 512
+E G+EIYPELHF+PASDIT+ D Q++Q+YL STKTP+AHL V T L VKPAP++A+FS
Sbjct: 526 IEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLTSVKTLLSVKPAPIIATFS 585
Query: 513 SRGPNPIESSILKPDITAPGVNILASYPTGKAPTSSISDRRRIPFNVISGTSMSCPHISG 572
SRGPNPI+S+ILKPD+TAPGVNILASYPTG APT S +DRRRIPFNVISGTSMSCPH++G
Sbjct: 586 SRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRRIPFNVISGTSMSCPHVAG 645
Query: 573 IAALLKSIHPEWSPAAIKSAIVTTAKTRGNNLQTILDSNKLRASPFAYGAGLVNPNNAMD 632
IA L+KSIHP WSPAAIKSAI+TTAKTRGNN QTILDS KL+A+P+AYGAG V PN+A D
Sbjct: 646 IAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATPYAYGAGQVYPNDAAD 705
Query: 633 PGLVYDTTVDDYLNFLCARGYNATQIRQFDTKPFICSKSFKLTDLNYPSISVGELKIGAP 692
PGLVYD T++DYLNFLCARGY+A +I++F KPF C +SFK+TDLNYPSISVGELKIGAP
Sbjct: 706 PGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKVTDLNYPSISVGELKIGAP 765
Query: 693 VTINRRVKNVGNPGTYVARVKVSPGVAVSVEPSTLQFSSVGEEKAFKVVLQNTGKVTRNG 752
+TINRRVKNVG+PGTYVARVK SPGVAV++EPSTL F SVGEEK FKVVLQNTGKV ++G
Sbjct: 766 LTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGEEKGFKVVLQNTGKV-KSG 825
Query: 753 YVFGTLIWSDGKHFVRSPMAIDL 776
VFGTLIWSDGKHFVRSP+A+ L
Sbjct: 826 DVFGTLIWSDGKHFVRSPIAVHL 847
BLAST of Lcy11g016530 vs. ExPASy TrEMBL
Match:
A0A0A0L241 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045090 PE=3 SV=1)
HSP 1 Score: 1228.4 bits (3177), Expect = 0.0e+00
Identity = 594/744 (79.84%), Postives = 667/744 (89.65%), Query Frame = 0
Query: 32 KSYIVYLGSQLSSSLGPSALSEQSRQVTASHYDLLGSMLGSKTMAQEAIFYSYTRSFNGF 91
+SYIVYLGS+ SS+L PS+LSE SRQVTA HYDLLGS+ GSKTMA+EAIFYSYTRSFNGF
Sbjct: 2 QSYIVYLGSEHSSALDPSSLSEHSRQVTALHYDLLGSLFGSKTMAEEAIFYSYTRSFNGF 61
Query: 92 AAKLDEKEAADLARNPKVISVFENKPRKLHTTRSWNFLGLENDAGIPSNSIWNAAKFGQD 151
AAKLD+KEA +LARNPKVISVFENK RKLHTTRSWNFLG+END GIPSNSIWN AKFG+D
Sbjct: 62 AAKLDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPSNSIWNTAKFGED 121
Query: 152 VILANVDTGVWPESQSFSDEGYGPVPSKWRGICQSDSTFHCNKKLIGARYFYKGYEAAGG 211
VI+AN+DTGVWPES+SFSDEGYGPVPSKWRGICQ+DSTFHCN+KLIG RYF+KGYEAAGG
Sbjct: 122 VIVANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSTFHCNRKLIGGRYFHKGYEAAGG 181
Query: 212 VLNASLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARVAAYKVCWPSF 271
LNA+LLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKAR AYK CWP
Sbjct: 182 KLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARAVAYKACWPPL 241
Query: 272 MDGQCFDADTLAAFEAAIADGVDVISVSLGGTANEFVNDPLSIVAFHAVQQGIVVVFSAG 331
D QCFDAD LAAFEAAIADGVDV+S SLGG A+E+ NDPL+I AF AVQ+GI+VVFS G
Sbjct: 242 FDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLAVQRGILVVFSGG 301
Query: 332 NSGPFPGTVTNVAPWQITVAASTDDRDFASYVALGNKKHLKGVSLSSVAPLPKKFYPLIT 391
NSGPFP T+ N++PW TVAAST DR+FASYV LGNKKH+KG+SLSSV LPKKF+PLI
Sbjct: 302 NSGPFPMTIANISPWVFTVAASTIDREFASYVGLGNKKHIKGLSLSSVPSLPKKFFPLIN 361
Query: 392 SVDARFSNVTEYHAQFCDEGTLDPIKVKGKILICLSGEIEGIDKGFQAARAGAVGVVVAN 451
SVDA+F NVTE+HAQFC +GTLDP+KVKGKI+IC GE +G+DKGFQA+RAGAVGV++AN
Sbjct: 362 SVDAKFRNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETDGVDKGFQASRAGAVGVIIAN 421
Query: 452 DLEVGNEIYPELHFLPASDITSNDNQLLQQYLNSTKTPVAHLKKVITQLGVKPAPVMASF 511
DLE G+EI+PELHF+PASDIT+ D Q++Q YL ST+TP+AHL V T L VKPAP +A+F
Sbjct: 422 DLEKGDEIFPELHFIPASDITNTDAQIVQNYLKSTRTPMAHLTSVKTLLSVKPAPTIATF 481
Query: 512 SSRGPNPIESSILKPDITAPGVNILASYPTGKAPTSSISDRRRIPFNVISGTSMSCPHIS 571
S+RGPNPI+S+ILKPD+TAPGVNILASYPTG APT S DRRRIPFNVISGTSMSCPH++
Sbjct: 482 SARGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSPVDRRRIPFNVISGTSMSCPHVA 541
Query: 572 GIAALLKSIHPEWSPAAIKSAIVTTAKTRGNNLQTILDSNKLRASPFAYGAGLVNPNNAM 631
GIA L+KSIHP WSPAAIKSAI+TTAKTRGNN QTILDS KL+A+P+AYGAG VNPN+A
Sbjct: 542 GIAGLIKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATPYAYGAGQVNPNDAA 601
Query: 632 DPGLVYDTTVDDYLNFLCARGYNATQIRQFDTKPFICSKSFKLTDLNYPSISVGELKIGA 691
DPGLVYD TV+DYLNFLCARGYNA QI++F KPF C +SFK+TDLNYPSISVGELKIGA
Sbjct: 602 DPGLVYDITVNDYLNFLCARGYNAMQIKKFYAKPFSCVRSFKVTDLNYPSISVGELKIGA 661
Query: 692 PVTINRRVKNVGNPGTYVARVKVSPGVAVSVEPSTLQFSSVGEEKAFKVVLQNTGKVTRN 751
P+T+NRRVKNVG+PGTYVARVK SPGVAVS+EPSTL FS VGEEK FKVVLQNTGKV
Sbjct: 662 PLTMNRRVKNVGSPGTYVARVKASPGVAVSIEPSTLVFSRVGEEKGFKVVLQNTGKVKNG 721
Query: 752 GYVFGTLIWSDGKHFVRSPMAIDL 776
VFGTLIWSDGKHFVRS +A+ L
Sbjct: 722 SDVFGTLIWSDGKHFVRSSIAVHL 745
BLAST of Lcy11g016530 vs. ExPASy TrEMBL
Match:
A0A5D3D763 (Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00150 PE=3 SV=1)
HSP 1 Score: 1188.3 bits (3073), Expect = 0.0e+00
Identity = 594/775 (76.65%), Postives = 668/775 (86.19%), Query Frame = 0
Query: 13 LLLFFFLISLLQTSTIATEKSYIVYLGSQLSSSLGPSALSEQSRQVTASHYDLLGSMLGS 72
LLLFFF SLLQTSTIAT+KSYIVYLGS+ SSSL PS+LSE S+QVTA HYDLLGS LGS
Sbjct: 9 LLLFFFYFSLLQTSTIATKKSYIVYLGSEHSSSLDPSSLSEHSQQVTALHYDLLGSSLGS 68
Query: 73 KTMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPKVISVFENKPRKLHTTRSWNFLGLE 132
KTMA+EAIFYSYTRSFNGFAA LD+KEA +LARNPKVISVFENK RKLHTTRSWNFLG+E
Sbjct: 69 KTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVE 128
Query: 133 NDAGIPSNSIWNAAKFGQDVILANVDTGVWPESQSFSDEGYGPVPSKWRGICQSDSTFHC 192
ND GIPSNSIWNAAKFG+DVI+AN+DTGVWPES+SFSDEGYGPVPSKWRGICQ+DS F C
Sbjct: 129 NDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFLC 188
Query: 193 NKKLIGARYFYKGYEAAGGVLNASLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTA 252
N+KLIG RYF+KGYEAAGG LNA+LLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTA
Sbjct: 189 NRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTA 248
Query: 253 KGGAPKARVAAYKVCWPSFMDGQCFDADTLAAFEAAIADGVDVISVSLGGTANEFVNDPL 312
KGGAPKAR AYKVCWP D QCFDAD LAAFEAAIADGVDV+S SLGG A+E+ NDPL
Sbjct: 249 KGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPL 308
Query: 313 SIVAFHAVQQGIVVVFSAGNSGPFPGTVTNVAPWQITVAASTDDRDFASYVALGNKKHLK 372
+I AF AVQ+GI+VVFS GNSGPFP TVTNV+PW TVAA+T DRDF SYV LGNKKH+K
Sbjct: 309 AIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIK 368
Query: 373 --------GVSLSSVAP----LPKKFYPLITSVDARFSNVTEYHAQFCDEGTLDPIKVKG 432
G+ S AP P+ + SN QFC +GTLDP+KVKG
Sbjct: 369 VHVYTHLLGLGASRFAPNFICAPELYNQYSIGSTLETSN------QFCGKGTLDPMKVKG 428
Query: 433 KILICLSGEIEGIDKGFQAARAGAVGVVVANDLEVGNEIYPELHFLPASDITSNDNQLLQ 492
KI+IC GE EG+DKG+QA+ AGA GV+VAND+E G+EIYPELHF+PASDIT+ D Q++Q
Sbjct: 429 KIVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQ 488
Query: 493 QYLNSTKTPVAHLKKVITQLGVKPAPVMASFSSRGPNPIESSILKPDITAPGVNILASYP 552
+YL STKTP+AHL V T L VKPAP++A+FSSRGPNPI+S+ILKPD+TAPGVNILASYP
Sbjct: 489 KYLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYP 548
Query: 553 TGKAPTSSISDRRRIPFNVISGTSMSCPHISGIAALLKSIHPEWSPAAIKSAIVTTAKTR 612
TG APT S +DRRRIPFNVISGTSMSCPH++GIA L+KSIHP WSPAAIKSAI+TTAKTR
Sbjct: 549 TGIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTR 608
Query: 613 GNNLQTILDSNKLRASPFAYGAGLVNPNNAMDPGLVYDTTVDDYLNFLCARGYNATQIRQ 672
GNN QTILDS KL+A+P+AYGAG V PN+A DPGLVYD T++DYLNFLCARGY+A +I++
Sbjct: 609 GNNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKK 668
Query: 673 FDTKPFICSKSFKLTDLNYPSISVGELKIGAPVTINRRVKNVGNPGTYVARVKVSPGVAV 732
F KPF C +SFK+TDLNYPSISVGELKIGAP+TINRRVKNVG+PGTYVARVK SPGVAV
Sbjct: 669 FYAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAV 728
Query: 733 SVEPSTLQFSSVGEEKAFKVVLQNTGKVTRNGYVFGTLIWSDGKHFVRSPMAIDL 776
++EPSTL F SVGEEK FKVVLQNTGKV ++G VFGTLIWSDGKHFVRSP+A+ L
Sbjct: 729 TIEPSTLVFRSVGEEKGFKVVLQNTGKV-KSGDVFGTLIWSDGKHFVRSPIAVHL 776
BLAST of Lcy11g016530 vs. ExPASy TrEMBL
Match:
A0A6J1CQS4 (subtilisin-like protease SBT5.3 OS=Momordica charantia OX=3673 GN=LOC111013337 PE=3 SV=1)
HSP 1 Score: 1147.5 bits (2967), Expect = 0.0e+00
Identity = 573/768 (74.61%), Postives = 658/768 (85.68%), Query Frame = 0
Query: 13 LLLFFFLISLLQTSTIATE-KSYIVYLGSQLSSSLGPSALSEQSRQVTASHYDLLGSMLG 72
LLL FFL+SLLQTSTIA + KSYIVYLGSQ SSL ++L + SRQ+TASHY+LLGS+LG
Sbjct: 9 LLLAFFLLSLLQTSTIAIDKKSYIVYLGSQ--SSLASTSLLD-SRQLTASHYNLLGSLLG 68
Query: 73 SKTMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPKVISVFENKPRKLHTTRSWNFLGL 132
SK +A+E+IFYSYTRSFNGFAA LDE AA LA+NP+VISVFEN+ RKLHTT SWNFLG+
Sbjct: 69 SKKIAEESIFYSYTRSFNGFAATLDENVAARLAKNPQVISVFENRARKLHTTHSWNFLGM 128
Query: 133 ENDAGI-PSNSIWNAAKFGQDVILANVDTGVWPESQSFSDEGYGPVPSKWRGICQSDSTF 192
EN G+ P NSIWNAAKFGQD+I+AN+DTGVWPES+SFSDEG+GP+PS+W+G CQ+ F
Sbjct: 129 ENAQGVPPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSRWKGTCQTYLNF 188
Query: 193 HCNKKLIGARYFYKGYEAAGGVLNASLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNG 252
HCN+KLIG RYFYKGYEAAG L+ S LTVRDHDGHGTHTL+TAAGNFV GANVFG GNG
Sbjct: 189 HCNRKLIGGRYFYKGYEAAGAKLSPSSLTVRDHDGHGTHTLATAAGNFVPGANVFGQGNG 248
Query: 253 TAKGGAPKARVAAYKVCWPSFMDGQCFDADTLAAFEAAIADGVDVISVSLGGTANEFVND 312
TAKGGAP+ARVAAYKVCWP +G+CFDADTLA FEAAIADGVDVIS SLGG+ +F +D
Sbjct: 249 TAKGGAPRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGGSVRDFADD 308
Query: 313 PLSIVAFHAVQQGIVVVFSAGNSGPFPGTVTNVAPWQITVAASTDDRDFASYVALGNKKH 372
PL+I AFHA+QQGIV VFSAGN GP PG+V+NVAPW+ TVAAST DRDFASY+ALGNKK
Sbjct: 309 PLAIAAFHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASYIALGNKKR 368
Query: 373 LKGVSLSSVAPLPKKFYPLITSVDARF--SNVTEYHAQFCDEGTLDPIKVKGKILICLSG 432
+KG SL+SVAPLPKKFYPLI SV R NVT+Y AQFC EGT DP KVKGKIL+CL G
Sbjct: 369 IKGASLASVAPLPKKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKVKGKILVCLEG 428
Query: 433 EIEGIDKGFQAARAGAVGVVVANDLEVGNEIYPELHFLPASDITSNDNQLLQQYLNSTKT 492
EI G +KG +A R GA G++V ND + G++I+PELHFLPASD+ +D Q+L QY++ST+
Sbjct: 429 EIVGFEKGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQVLTQYMDSTRN 488
Query: 493 PVAHLKKVITQLGVKPAPVMASFSSRGPNPIESSILKPDITAPGVNILASYPTGKAPTSS 552
PV HL KV T+LG+KPAPVMA FSSRGPN IE SILKPDITAPGVNILA+Y +G APT+S
Sbjct: 489 PVGHLMKVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAAYSSGNAPTTS 548
Query: 553 ISDRRRIPFNVISGTSMSCPHISGIAALLKSIHPEWSPAAIKSAIVTTAKTRGNNLQTIL 612
DRRRIPF+VISGTSMSCPHISGIAALLKSIHP+WSPAAIKSAI+TTAKTR NNL +IL
Sbjct: 549 RYDRRRIPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAKTRDNNLGSIL 608
Query: 613 DSNKLRASPFAYGAGLVNPNNAMDPGLVYDTTVDDYLNFLCARGYNATQIRQFDTKPFIC 672
D K++A+PFAYGAG V+PN+AMDPGLVYDTTV DYLNFLCARGY A QIR+F K ++C
Sbjct: 609 DLTKVKATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQIRRFYAKSYVC 668
Query: 673 SKSFKLTDLNYPSISVGELKIGAPVTINRRVKNVGNPGTYVARVKVSPGVAVSVEPSTLQ 732
+SFKLTDLNYPSISV L +G PVT+NRRVKNVG+ GTYVARVKV P V++SVEPSTLQ
Sbjct: 669 DRSFKLTDLNYPSISVSNLTMG-PVTVNRRVKNVGSLGTYVARVKVHPEVSISVEPSTLQ 728
Query: 733 FSSVGEEKAFKVVLQNTGKVTRNGYVFGTLIWSDGKHFVRSPMAIDLE 777
FSSVGEEKAFKVV Q GK R G +FGTLIWSDGKHFVRSP+ ++LE
Sbjct: 729 FSSVGEEKAFKVVFQYKGKGKREGSMFGTLIWSDGKHFVRSPIVMNLE 772
BLAST of Lcy11g016530 vs. ExPASy TrEMBL
Match:
A0A6J1L3L8 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111498821 PE=3 SV=1)
HSP 1 Score: 1065.4 bits (2754), Expect = 1.1e-307
Identity = 534/778 (68.64%), Postives = 633/778 (81.36%), Query Frame = 0
Query: 1 MEANSNNKLPPLLLLFFFLISLLQTS---TIATEKSYIVYLGSQLSSSLGPSALSEQSRQ 60
MEA++ L P +L FFL SLLQTS TIAT+K YIVYL SQ S+ + +Q
Sbjct: 1 MEASN---LIPSILFLFFLFSLLQTSTIATIATKKPYIVYLESQ---SVYADSKHCSIQQ 60
Query: 61 VTASHYDLLGSMLGSKTMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPKVISVFENKP 120
V HY+LLGS+LGS A+E+I YSYTRSFNGFAA L+EKEAADLAR+P+VISV EN+
Sbjct: 61 VQEFHYNLLGSLLGSPEAAKESILYSYTRSFNGFAAILNEKEAADLARSPQVISVLENRG 120
Query: 121 RKLHTTRSWNFLGLENDAGIPSNSIWNAAKFGQDVILANVDTGVWPESQSFSDEGYGPVP 180
RKLHTT SW FLG+E+D GIPSNSIWNAA+FG+D+I+AN+DTGVWPES SFSDEGYGP+P
Sbjct: 121 RKLHTTNSWRFLGVEDDEGIPSNSIWNAAQFGRDMIIANIDTGVWPESNSFSDEGYGPIP 180
Query: 181 SKWRGICQSDSTFHCNKKLIGARYFYKGYEAAGGVLNASLLTVRDHDGHGTHTLSTAAGN 240
SKWRG C D +F CN+KLIG RYFYKGY+ AGGVLNA+ +++RDH+GHGTHTLSTAAGN
Sbjct: 181 SKWRGTCPDDPSFRCNRKLIGGRYFYKGYKFAGGVLNATSVSIRDHNGHGTHTLSTAAGN 240
Query: 241 FVTGANVFGHGNGTAKGGAPKARVAAYKVCWPSFMDGQCFDADTLAAFEAAIADGVDVIS 300
FV GAN+FGHGNGTAKGGAPKARVAAYK CWP +G+CFDAD LAAFEAAI DGVDVIS
Sbjct: 241 FVPGANIFGHGNGTAKGGAPKARVAAYKACWPLIPEGECFDADVLAAFEAAINDGVDVIS 300
Query: 301 VSLGGTANEFVNDPLSIVAFHAVQQGIVVVFSAGNSGPFPGTVTNVAPWQITVAASTDDR 360
SLGG EF DP+ I AFHA QQGI+V+FSAGN GP P TV NVAPW+ITVAAST R
Sbjct: 301 ASLGGGDQEFFRDPVGIAAFHAAQQGIIVIFSAGNDGPQPETVGNVAPWEITVAASTTGR 360
Query: 361 DFASYVALGNKKHLKGVSLSSVAPLPKKFYPLITSVDARFSNVTEYHAQFCDEGTLDPIK 420
+F S VALGN K LKG SLSSV+ LP +FYPLI SV+A+FSNV+E+ A+FC +GTL+P K
Sbjct: 361 NFVSNVALGNNKILKGSSLSSVSALP-QFYPLIDSVNAKFSNVSEFQAKFCGKGTLNPKK 420
Query: 421 VKGKILICLSGEIEGIDKGFQAARAGAVGVVVANDLEVGNEIYPELHFLPASDITSNDNQ 480
VKGKILIC +G+I G++KG+ AA AGAVG+++A +++ EI PEL+FLPAS IT +D +
Sbjct: 421 VKGKILICTAGKITGVEKGYNAAEAGAVGMILATNIDNLEEIKPELYFLPASGITYSDGE 480
Query: 481 LLQQYLNSTKTPVAHLKKVITQLGVKPAPVMASFSSRGPNPIESSILKPDITAPGVNILA 540
LL Y+NST TPVA + V T++ + P+PV+A+FSSRGPNP + +ILKPDITAPG ILA
Sbjct: 481 LLDDYINSTSTPVAQIMNVRTEVEINPSPVVAAFSSRGPNPFDKAILKPDITAPGFTILA 540
Query: 541 SYPTGKAPTSSISDRRRIPFNVISGTSMSCPHISGIAALLKSIHPEWSPAAIKSAIVTTA 600
SYPT +APT S D+RR PFNV SGTSM+CPHIS IAALLKSIHPEWSPAAIKSA++TTA
Sbjct: 541 SYPTTRAPTRSGFDQRRTPFNVFSGTSMACPHISAIAALLKSIHPEWSPAAIKSALMTTA 600
Query: 601 KTRGNNLQTILDSNKLRASPFAYGAGLVNPNNAMDPGLVYDTTVDDYLNFLCARGYNATQ 660
KT NN + L A+PFA+GAG V PN+AMDPGLVYD TVD+YLNFLCARGYNA Q
Sbjct: 601 KTSDNN------NLLLNATPFAFGAGHVRPNDAMDPGLVYDITVDEYLNFLCARGYNAIQ 660
Query: 661 IRQFDTKPFICSKSFKLTDLNYPSISVGELKIGAPVTINRRVKNVGNPGTYVARVKVSPG 720
+R+F +PF+C KSFK DLNYPSIS+ L + APVTINRRVKNVG PGTYVARV++ G
Sbjct: 661 LRRFSKQPFVCEKSFKAIDLNYPSISIPNLNVNAPVTINRRVKNVGGPGTYVARVELPEG 720
Query: 721 VAVSVEPSTLQFSSVGEEKAFKVVLQNTGKVTRNGYVFGTLIWSDGKHFVRSPMAIDL 776
VA SVEPSTLQFSSVGEEKAF+VV+QNTG++ GYVFG L+WSDGKH V SP++++L
Sbjct: 721 VAASVEPSTLQFSSVGEEKAFRVVVQNTGELKHEGYVFGKLVWSDGKHSVGSPISMNL 765
BLAST of Lcy11g016530 vs. NCBI nr
Match:
XP_038904203.1 (uncharacterized protein LOC120090550 [Benincasa hispida])
HSP 1 Score: 1260.0 bits (3259), Expect = 0.0e+00
Identity = 623/763 (81.65%), Postives = 692/763 (90.69%), Query Frame = 0
Query: 13 LLLFFFLISLLQTSTIATEKSYIVYLGSQLSSSLGPSALSEQSRQVTASHYDLLGSMLGS 72
LLLFFF SLLQTS IAT+KSYIVYLGS+ SSSL PS+LSE SRQVTA HYDLLG++LGS
Sbjct: 9 LLLFFFYFSLLQTSIIATKKSYIVYLGSERSSSLDPSSLSEHSRQVTAFHYDLLGTLLGS 68
Query: 73 KTMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPKVISVFENKPRKLHTTRSWNFLGLE 132
+ M +EAIFYSYT SFNGFAAKLDEKEAA+LARNPKVISVFENK RKLHTTRSWNFLG+E
Sbjct: 69 QKMTEEAIFYSYTTSFNGFAAKLDEKEAANLARNPKVISVFENKARKLHTTRSWNFLGVE 128
Query: 133 NDAGIPSNSIWNAAKFGQDVILANVDTGVWPESQSFSDEGYGPVPSKWRGICQSDSTFHC 192
ND GIPSNSIWNAAKFGQD+I+AN+DTGVWPES+SFSDEGYGPVPSKWRGICQ+DS FHC
Sbjct: 129 NDVGIPSNSIWNAAKFGQDIIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHC 188
Query: 193 NKKLIGARYFYKGYEAAGGVLNASLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTA 252
N+KLIG RYFYKGY AAGG LNA+ LTVRDHDGHGTHTLSTAAGNFVTGANVFGHG+GTA
Sbjct: 189 NRKLIGGRYFYKGYVAAGGTLNATSLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGSGTA 248
Query: 253 KGGAPKARVAAYKVCWPSFMDGQCFDADTLAAFEAAIADGVDVISVSLGGTANEFVNDPL 312
KGGAPKARVAAYKVCWP F+ QC DAD LAAFEAA+ADGVDVIS SLGG A+E+ NDPL
Sbjct: 249 KGGAPKARVAAYKVCWPPFLGSQCLDADILAAFEAAVADGVDVISTSLGGAADEYFNDPL 308
Query: 313 SIVAFHAVQQGIVVVFSAGNSGPFPGTVTNVAPWQITVAASTDDRDFASYVALGNKKHLK 372
+I AFHAVQQG+VVVFSAGNSGPFP TVTN+APW TVAA T DRDFAS VALGNK +
Sbjct: 309 AIAAFHAVQQGVVVVFSAGNSGPFPMTVTNIAPWINTVAAGTVDRDFASNVALGNK---E 368
Query: 373 GVSLSSVAPLPKKFYPLITSVDARFSNVTEYHAQFCDEGTLDPIKVKGKILICLSGEIEG 432
GVSLSS+APLPKKFYPLI SV+A+ SNVTE+HA+FC EGTLDP+KVKGKI+IC GEIEG
Sbjct: 369 GVSLSSIAPLPKKFYPLIDSVNAKLSNVTEFHARFCGEGTLDPMKVKGKIVICQVGEIEG 428
Query: 433 IDKGFQAARAGAVGVVVANDLEVGNEIYPELHFLPASDITSNDNQLLQQYLNSTKTPVAH 492
++K +QAARAGAVGV+VAND+E G+EIYPELHF+PASDIT+ND QLLQ+YLNST TP+AH
Sbjct: 429 VEKSYQAARAGAVGVIVANDIEKGDEIYPELHFIPASDITNNDAQLLQKYLNSTTTPMAH 488
Query: 493 LKKVITQLGVKPAPVMASFSSRGPNPIESSILKPDITAPGVNILASYPTGKAPTSSISDR 552
L KV T L +KPAP++A+FSSRGPNPI+ ILKPDITAPGVNILASY TG APT S +D+
Sbjct: 489 LTKVKTLLNIKPAPIIATFSSRGPNPIDPFILKPDITAPGVNILASYSTGNAPTFSSNDQ 548
Query: 553 RRIPFNVISGTSMSCPHISGIAALLKSIHPEWSPAAIKSAIVTTAKTRGNNLQTILDSNK 612
RRIPFNVISGTSMSCPHI+GIA LLKSIHP+WSPAAIKSAI+TTAKTRGNNLQTILDS K
Sbjct: 549 RRIPFNVISGTSMSCPHIAGIAGLLKSIHPDWSPAAIKSAIMTTAKTRGNNLQTILDSTK 608
Query: 613 LRASPFAYGAGLVNPNNAMDPGLVYDTTVDDYLNFLCARGYNATQIRQFDTKPFICSKSF 672
L+A+ +AYGAG+V+PN+A DPGLVYDTT++DYLNFLCARGYNA ++++F KPF C KSF
Sbjct: 609 LKATSYAYGAGMVHPNDARDPGLVYDTTIEDYLNFLCARGYNAMEMKKFYAKPFNCVKSF 668
Query: 673 KLTDLNYPSISVGELKIGAPVTINRRVKNVGNPGTYVARVKVSPGVAVSVEPSTLQFSSV 732
K TDLNYPSISVG L+IGAPVTINRRVK+VG+PGTYVARVKVSPGVAV VEP TLQFSSV
Sbjct: 669 KATDLNYPSISVGVLRIGAPVTINRRVKSVGSPGTYVARVKVSPGVAVLVEPRTLQFSSV 728
Query: 733 GEEKAFKVVLQNTGKVTRNGYVFGTLIWSDGKHFVRSPMAIDL 776
GEEKAFKVVLQNTGKV R G VFGTLIWSDGKHFVRSP+A+ L
Sbjct: 729 GEEKAFKVVLQNTGKVKR-GDVFGTLIWSDGKHFVRSPIAVHL 767
BLAST of Lcy11g016530 vs. NCBI nr
Match:
XP_016900593.1 (PREDICTED: subtilisin-like protease SBT5.3 [Cucumis melo])
HSP 1 Score: 1231.9 bits (3186), Expect = 0.0e+00
Identity = 600/743 (80.75%), Postives = 672/743 (90.44%), Query Frame = 0
Query: 33 SYIVYLGSQLSSSLGPSALSEQSRQVTASHYDLLGSMLGSKTMAQEAIFYSYTRSFNGFA 92
SYIVYLGS+ SSSL PS+LSE S+QVTA HYDLLGS LGSKTMA+EAIFYSYTRSFNGFA
Sbjct: 106 SYIVYLGSEHSSSLDPSSLSEHSQQVTALHYDLLGSSLGSKTMAEEAIFYSYTRSFNGFA 165
Query: 93 AKLDEKEAADLARNPKVISVFENKPRKLHTTRSWNFLGLENDAGIPSNSIWNAAKFGQDV 152
A LD+KEA +LARNPKVISVFENK RKLHTTRSWNFLG+END GIPSNSIWNAAKFG+DV
Sbjct: 166 AILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPSNSIWNAAKFGEDV 225
Query: 153 ILANVDTGVWPESQSFSDEGYGPVPSKWRGICQSDSTFHCNKKLIGARYFYKGYEAAGGV 212
I+AN+DTGVWPES+SFSDEGYGPVPSKWRGICQ+DS FHCN+KLIG RYF+KGYEAAGG
Sbjct: 226 IIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNRKLIGGRYFHKGYEAAGGK 285
Query: 213 LNASLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARVAAYKVCWPSFM 272
LNA+LLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKAR AYKVCWP
Sbjct: 286 LNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARAVAYKVCWPPLF 345
Query: 273 DGQCFDADTLAAFEAAIADGVDVISVSLGGTANEFVNDPLSIVAFHAVQQGIVVVFSAGN 332
D QCFDAD LAAFEAAIADGVDV+S SLGG A+E+ NDPL+I AF AVQ+GI+VVFS GN
Sbjct: 346 DSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLAVQRGILVVFSGGN 405
Query: 333 SGPFPGTVTNVAPWQITVAASTDDRDFASYVALGNKKHLKGVSLSSVAPLPKKFYPLITS 392
SGPFP TVTNV+PW TVAA+T DRDF SYV LGNKKH+KGVSLSSVA LPKKFYPLI S
Sbjct: 406 SGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGVSLSSVASLPKKFYPLINS 465
Query: 393 VDARFSNVTEYHAQFCDEGTLDPIKVKGKILICLSGEIEGIDKGFQAARAGAVGVVVAND 452
VDA+FSNVTE+HAQFC +GTLDP+KVKGKI+IC GE EG+DKG+QA+ AGA GV+VAND
Sbjct: 466 VDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVDKGYQASLAGAAGVIVAND 525
Query: 453 LEVGNEIYPELHFLPASDITSNDNQLLQQYLNSTKTPVAHLKKVITQLGVKPAPVMASFS 512
+E G+EIYPELHF+PASDIT+ D Q++Q+YL STKTP+AHL V T L VKPAP++A+FS
Sbjct: 526 IEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLTSVKTLLSVKPAPIIATFS 585
Query: 513 SRGPNPIESSILKPDITAPGVNILASYPTGKAPTSSISDRRRIPFNVISGTSMSCPHISG 572
SRGPNPI+S+ILKPD+TAPGVNILASYPTG APT S +DRRRIPFNVISGTSMSCPH++G
Sbjct: 586 SRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRRIPFNVISGTSMSCPHVAG 645
Query: 573 IAALLKSIHPEWSPAAIKSAIVTTAKTRGNNLQTILDSNKLRASPFAYGAGLVNPNNAMD 632
IA L+KSIHP WSPAAIKSAI+TTAKTRGNN QTILDS KL+A+P+AYGAG V PN+A D
Sbjct: 646 IAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATPYAYGAGQVYPNDAAD 705
Query: 633 PGLVYDTTVDDYLNFLCARGYNATQIRQFDTKPFICSKSFKLTDLNYPSISVGELKIGAP 692
PGLVYD T++DYLNFLCARGY+A +I++F KPF C +SFK+TDLNYPSISVGELKIGAP
Sbjct: 706 PGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKVTDLNYPSISVGELKIGAP 765
Query: 693 VTINRRVKNVGNPGTYVARVKVSPGVAVSVEPSTLQFSSVGEEKAFKVVLQNTGKVTRNG 752
+TINRRVKNVG+PGTYVARVK SPGVAV++EPSTL F SVGEEK FKVVLQNTGKV ++G
Sbjct: 766 LTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGEEKGFKVVLQNTGKV-KSG 825
Query: 753 YVFGTLIWSDGKHFVRSPMAIDL 776
VFGTLIWSDGKHFVRSP+A+ L
Sbjct: 826 DVFGTLIWSDGKHFVRSPIAVHL 847
BLAST of Lcy11g016530 vs. NCBI nr
Match:
XP_031738719.1 (uncharacterized protein LOC101212014 [Cucumis sativus])
HSP 1 Score: 1227.6 bits (3175), Expect = 0.0e+00
Identity = 594/743 (79.95%), Postives = 666/743 (89.64%), Query Frame = 0
Query: 33 SYIVYLGSQLSSSLGPSALSEQSRQVTASHYDLLGSMLGSKTMAQEAIFYSYTRSFNGFA 92
SYIVYLGS+ SS+L PS+LSE SRQVTA HYDLLGS+ GSKTMA+EAIFYSYTRSFNGFA
Sbjct: 796 SYIVYLGSEHSSALDPSSLSEHSRQVTALHYDLLGSLFGSKTMAEEAIFYSYTRSFNGFA 855
Query: 93 AKLDEKEAADLARNPKVISVFENKPRKLHTTRSWNFLGLENDAGIPSNSIWNAAKFGQDV 152
AKLD+KEA +LARNPKVISVFENK RKLHTTRSWNFLG+END GIPSNSIWN AKFG+DV
Sbjct: 856 AKLDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPSNSIWNTAKFGEDV 915
Query: 153 ILANVDTGVWPESQSFSDEGYGPVPSKWRGICQSDSTFHCNKKLIGARYFYKGYEAAGGV 212
I+AN+DTGVWPES+SFSDEGYGPVPSKWRGICQ+DSTFHCN+KLIG RYF+KGYEAAGG
Sbjct: 916 IVANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSTFHCNRKLIGGRYFHKGYEAAGGK 975
Query: 213 LNASLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARVAAYKVCWPSFM 272
LNA+LLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKAR AYK CWP
Sbjct: 976 LNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARAVAYKACWPPLF 1035
Query: 273 DGQCFDADTLAAFEAAIADGVDVISVSLGGTANEFVNDPLSIVAFHAVQQGIVVVFSAGN 332
D QCFDAD LAAFEAAIADGVDV+S SLGG A+E+ NDPL+I AF AVQ+GI+VVFS GN
Sbjct: 1036 DSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLAVQRGILVVFSGGN 1095
Query: 333 SGPFPGTVTNVAPWQITVAASTDDRDFASYVALGNKKHLKGVSLSSVAPLPKKFYPLITS 392
SGPFP T+ N++PW TVAAST DR+FASYV LGNKKH+KG+SLSSV LPKKF+PLI S
Sbjct: 1096 SGPFPMTIANISPWVFTVAASTIDREFASYVGLGNKKHIKGLSLSSVPSLPKKFFPLINS 1155
Query: 393 VDARFSNVTEYHAQFCDEGTLDPIKVKGKILICLSGEIEGIDKGFQAARAGAVGVVVAND 452
VDA+F NVTE+HAQFC +GTLDP+KVKGKI+IC GE +G+DKGFQA+RAGAVGV++AND
Sbjct: 1156 VDAKFRNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETDGVDKGFQASRAGAVGVIIAND 1215
Query: 453 LEVGNEIYPELHFLPASDITSNDNQLLQQYLNSTKTPVAHLKKVITQLGVKPAPVMASFS 512
LE G+EI+PELHF+PASDIT+ D Q++Q YL ST+TP+AHL V T L VKPAP +A+FS
Sbjct: 1216 LEKGDEIFPELHFIPASDITNTDAQIVQNYLKSTRTPMAHLTSVKTLLSVKPAPTIATFS 1275
Query: 513 SRGPNPIESSILKPDITAPGVNILASYPTGKAPTSSISDRRRIPFNVISGTSMSCPHISG 572
+RGPNPI+S+ILKPD+TAPGVNILASYPTG APT S DRRRIPFNVISGTSMSCPH++G
Sbjct: 1276 ARGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSPVDRRRIPFNVISGTSMSCPHVAG 1335
Query: 573 IAALLKSIHPEWSPAAIKSAIVTTAKTRGNNLQTILDSNKLRASPFAYGAGLVNPNNAMD 632
IA L+KSIHP WSPAAIKSAI+TTAKTRGNN QTILDS KL+A+P+AYGAG VNPN+A D
Sbjct: 1336 IAGLIKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATPYAYGAGQVNPNDAAD 1395
Query: 633 PGLVYDTTVDDYLNFLCARGYNATQIRQFDTKPFICSKSFKLTDLNYPSISVGELKIGAP 692
PGLVYD TV+DYLNFLCARGYNA QI++F KPF C +SFK+TDLNYPSISVGELKIGAP
Sbjct: 1396 PGLVYDITVNDYLNFLCARGYNAMQIKKFYAKPFSCVRSFKVTDLNYPSISVGELKIGAP 1455
Query: 693 VTINRRVKNVGNPGTYVARVKVSPGVAVSVEPSTLQFSSVGEEKAFKVVLQNTGKVTRNG 752
+T+NRRVKNVG+PGTYVARVK SPGVAVS+EPSTL FS VGEEK FKVVLQNTGKV
Sbjct: 1456 LTMNRRVKNVGSPGTYVARVKASPGVAVSIEPSTLVFSRVGEEKGFKVVLQNTGKVKNGS 1515
Query: 753 YVFGTLIWSDGKHFVRSPMAIDL 776
VFGTLIWSDGKHFVRS +A+ L
Sbjct: 1516 DVFGTLIWSDGKHFVRSSIAVHL 1538
BLAST of Lcy11g016530 vs. NCBI nr
Match:
KAE8650095.1 (hypothetical protein Csa_011146 [Cucumis sativus])
HSP 1 Score: 1213.4 bits (3138), Expect = 0.0e+00
Identity = 594/762 (77.95%), Postives = 666/762 (87.40%), Query Frame = 0
Query: 32 KSYIVYLGSQLSSSLGPSALSEQSRQVTASHYDLLGSMLGSKTMAQEAIFYSYTRSFNGF 91
+SYIVYLGS+ SS+L PS+LSE SRQVTA HYDLLGS+ GSKTMA+EAIFYSYTRSFNGF
Sbjct: 2 QSYIVYLGSEHSSALDPSSLSEHSRQVTALHYDLLGSLFGSKTMAEEAIFYSYTRSFNGF 61
Query: 92 AAKLDEKEAADLARNPKVISVFENKPRKLHTTRSWNFLGLENDAGIPSNSIWNAAKFGQD 151
AAKLD+KEA +LARNPKVISVFENK RKLHTTRSWNFLG+END GIPSNSIWN AKFG+D
Sbjct: 62 AAKLDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPSNSIWNTAKFGED 121
Query: 152 VILANVDTGVWPESQSFSDEGYGPVPSKWRGICQSDSTFHCNKKLIGARYFYKGYEAAGG 211
VI+AN+DTGVWPES+SFSDEGYGPVPSKWRGICQ+DSTFHCN+KLIG RYF+KGYEAAGG
Sbjct: 122 VIVANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSTFHCNRKLIGGRYFHKGYEAAGG 181
Query: 212 VLNASLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARVAAYKVCWPSF 271
LNA+LLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKAR AYK CWP
Sbjct: 182 KLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARAVAYKACWPPL 241
Query: 272 MDGQCFDADTLAAFEAAIADGVDVISVSLGGTANEFVNDPLSIVAFHAVQQGIVVVFSAG 331
D QCFDAD LAAFEAAIADGVDV+S SLGG A+E+ NDPL+I AF AVQ+GI+VVFS G
Sbjct: 242 FDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLAVQRGILVVFSGG 301
Query: 332 NSGPFPGTVTNVAPWQITVAASTDDRDFASYVALGNKKHLKGVSLSSVAPLPKKFYPLIT 391
NSGPFP T+ N++PW TVAAST DR+FASYV LGNKKH+KG+SLSSV LPKKF+PLI
Sbjct: 302 NSGPFPMTIANISPWVFTVAASTIDREFASYVGLGNKKHIKGLSLSSVPSLPKKFFPLIN 361
Query: 392 SVDARFSNVTEYHAQFCDEGTLDPIKVKGKILICLSGEIEGIDKGFQAARAGAVGVVVAN 451
SVDA+F NVTE+HAQFC +GTLDP+KVKGKI+IC GE +G+DKGFQA+RAGAVGV++AN
Sbjct: 362 SVDAKFRNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETDGVDKGFQASRAGAVGVIIAN 421
Query: 452 DLEVGNEIYPELHFLPASDITSNDNQLLQQYLNSTKTPVAHLKKVITQLGVKPAPVMASF 511
DLE G+EI+PELHF+PASDIT+ D Q++Q YL ST+TP+AHL V T L VKPAP +A+F
Sbjct: 422 DLEKGDEIFPELHFIPASDITNTDAQIVQNYLKSTRTPMAHLTSVKTLLSVKPAPTIATF 481
Query: 512 SSRGPNPIESSILKPDITAPGVNILASYPTGKAPTSSISDRRRIPFNVISGTSMSCPHIS 571
S+RGPNPI+S+ILKPD+TAPGVNILASYPTG APT S DRRRIPFNVISGTSMSCPH++
Sbjct: 482 SARGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSPVDRRRIPFNVISGTSMSCPHVA 541
Query: 572 GIAALLKSIHPEWSPAAIKSAIVTT------AKTRGNNLQTILDSNKLRASPFAYGAGLV 631
GIA L+KSIHP WSPAAIKSAI+TT AKTRGNN QTILDS KL+A+P+AYGAG V
Sbjct: 542 GIAGLIKSIHPNWSPAAIKSAIMTTVTYSFAAKTRGNNNQTILDSTKLKATPYAYGAGQV 601
Query: 632 NPNNAMDPGLVYDTTVDDYLNFLCARGYNATQIRQFDTKPFICSKSFKLTDLNYPSISVG 691
NPN+A DPGLVYD TV+DYLNFLCARGYNA QI++F KPF C +SFK+TDLNYPSISVG
Sbjct: 602 NPNDAADPGLVYDITVNDYLNFLCARGYNAMQIKKFYAKPFSCVRSFKVTDLNYPSISVG 661
Query: 692 ELKIGAPVTINRRVKNVGNPGTYVARVKVSPGVAVSVEPSTLQFSSVGEEKAFKVVLQNT 751
ELKIGAP+T+NRRVKNVG+PGTYVARVK SPGVAVS+EPSTL FS VGEEK FKVVLQNT
Sbjct: 662 ELKIGAPLTMNRRVKNVGSPGTYVARVKASPGVAVSIEPSTLVFSRVGEEKGFKVVLQNT 721
Query: 752 GKVTRNGYVFGTLIWSDGKHFV-----------RSPMAIDLE 777
GKV VFGTLIWSDGKHF +P A DLE
Sbjct: 722 GKVKNGSDVFGTLIWSDGKHFSYIVYLGSHTHGSNPSAFDLE 763
BLAST of Lcy11g016530 vs. NCBI nr
Match:
TYK19384.1 (subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa])
HSP 1 Score: 1188.3 bits (3073), Expect = 0.0e+00
Identity = 594/775 (76.65%), Postives = 668/775 (86.19%), Query Frame = 0
Query: 13 LLLFFFLISLLQTSTIATEKSYIVYLGSQLSSSLGPSALSEQSRQVTASHYDLLGSMLGS 72
LLLFFF SLLQTSTIAT+KSYIVYLGS+ SSSL PS+LSE S+QVTA HYDLLGS LGS
Sbjct: 9 LLLFFFYFSLLQTSTIATKKSYIVYLGSEHSSSLDPSSLSEHSQQVTALHYDLLGSSLGS 68
Query: 73 KTMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPKVISVFENKPRKLHTTRSWNFLGLE 132
KTMA+EAIFYSYTRSFNGFAA LD+KEA +LARNPKVISVFENK RKLHTTRSWNFLG+E
Sbjct: 69 KTMAEEAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVE 128
Query: 133 NDAGIPSNSIWNAAKFGQDVILANVDTGVWPESQSFSDEGYGPVPSKWRGICQSDSTFHC 192
ND GIPSNSIWNAAKFG+DVI+AN+DTGVWPES+SFSDEGYGPVPSKWRGICQ+DS F C
Sbjct: 129 NDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFLC 188
Query: 193 NKKLIGARYFYKGYEAAGGVLNASLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTA 252
N+KLIG RYF+KGYEAAGG LNA+LLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTA
Sbjct: 189 NRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTA 248
Query: 253 KGGAPKARVAAYKVCWPSFMDGQCFDADTLAAFEAAIADGVDVISVSLGGTANEFVNDPL 312
KGGAPKAR AYKVCWP D QCFDAD LAAFEAAIADGVDV+S SLGG A+E+ NDPL
Sbjct: 249 KGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPL 308
Query: 313 SIVAFHAVQQGIVVVFSAGNSGPFPGTVTNVAPWQITVAASTDDRDFASYVALGNKKHLK 372
+I AF AVQ+GI+VVFS GNSGPFP TVTNV+PW TVAA+T DRDF SYV LGNKKH+K
Sbjct: 309 AIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIK 368
Query: 373 --------GVSLSSVAP----LPKKFYPLITSVDARFSNVTEYHAQFCDEGTLDPIKVKG 432
G+ S AP P+ + SN QFC +GTLDP+KVKG
Sbjct: 369 VHVYTHLLGLGASRFAPNFICAPELYNQYSIGSTLETSN------QFCGKGTLDPMKVKG 428
Query: 433 KILICLSGEIEGIDKGFQAARAGAVGVVVANDLEVGNEIYPELHFLPASDITSNDNQLLQ 492
KI+IC GE EG+DKG+QA+ AGA GV+VAND+E G+EIYPELHF+PASDIT+ D Q++Q
Sbjct: 429 KIVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQ 488
Query: 493 QYLNSTKTPVAHLKKVITQLGVKPAPVMASFSSRGPNPIESSILKPDITAPGVNILASYP 552
+YL STKTP+AHL V T L VKPAP++A+FSSRGPNPI+S+ILKPD+TAPGVNILASYP
Sbjct: 489 KYLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYP 548
Query: 553 TGKAPTSSISDRRRIPFNVISGTSMSCPHISGIAALLKSIHPEWSPAAIKSAIVTTAKTR 612
TG APT S +DRRRIPFNVISGTSMSCPH++GIA L+KSIHP WSPAAIKSAI+TTAKTR
Sbjct: 549 TGIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTR 608
Query: 613 GNNLQTILDSNKLRASPFAYGAGLVNPNNAMDPGLVYDTTVDDYLNFLCARGYNATQIRQ 672
GNN QTILDS KL+A+P+AYGAG V PN+A DPGLVYD T++DYLNFLCARGY+A +I++
Sbjct: 609 GNNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKK 668
Query: 673 FDTKPFICSKSFKLTDLNYPSISVGELKIGAPVTINRRVKNVGNPGTYVARVKVSPGVAV 732
F KPF C +SFK+TDLNYPSISVGELKIGAP+TINRRVKNVG+PGTYVARVK SPGVAV
Sbjct: 669 FYAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAV 728
Query: 733 SVEPSTLQFSSVGEEKAFKVVLQNTGKVTRNGYVFGTLIWSDGKHFVRSPMAIDL 776
++EPSTL F SVGEEK FKVVLQNTGKV ++G VFGTLIWSDGKHFVRSP+A+ L
Sbjct: 729 TIEPSTLVFRSVGEEKGFKVVLQNTGKV-KSGDVFGTLIWSDGKHFVRSPIAVHL 776
BLAST of Lcy11g016530 vs. TAIR 10
Match:
AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 832.4 bits (2149), Expect = 3.0e-241
Identity = 424/767 (55.28%), Postives = 545/767 (71.06%), Query Frame = 0
Query: 12 LLLLFFFLISLLQTSTIATEKSYIVYLGSQLSSSLGPSALSEQSRQVTASHYDLLGSMLG 71
LLLL +S SY+VY G+ S +G R V +HYD LGS G
Sbjct: 10 LLLLLLVHMSSKHILASKDSSSYVVYFGAH--SHVGEITEDAMDR-VKETHYDFLGSFTG 69
Query: 72 SKTMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPKVISVFENKPRKLHTTRSWNFLGL 131
S+ A +AIFYSYT+ NGFAA LD A +++++P+V+SVF NK KLHTTRSW+FLGL
Sbjct: 70 SRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGL 129
Query: 132 ENDAGIPSNSIWNAAKFGQDVILANVDTGVWPESQSFSDEGYGPVPSKWRGICQS--DST 191
E+++ +PS+SIW A+FG+D I+AN+DTGVWPES+SF DEG GP+PS+W+GICQ+ D+T
Sbjct: 130 EHNSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDAT 189
Query: 192 FHCNKKLIGARYFYKGYEAAGGVLNASLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGN 251
FHCN+KLIGARYF KGY AA G LN+S + RD DGHG+HTLSTAAG+FV G ++FG GN
Sbjct: 190 FHCNRKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGN 249
Query: 252 GTAKGGAPKARVAAYKVCWPSFMDGQCFDADTLAAFEAAIADGVDVISVSLGGTANEFVN 311
GTAKGG+P+ARVAAYKVCWP +C+DAD LAAF+AAI DG DVISVSLGG F N
Sbjct: 250 GTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFN 309
Query: 312 DPLSIVAFHAVQQGIVVVFSAGNSGPFPGTVTNVAPWQITVAASTDDRDFASYVALGNKK 371
D ++I +FHA ++ IVVV SAGNSGP TV+NVAPWQITV AST DR+FAS + LGN K
Sbjct: 310 DSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGK 369
Query: 372 HLKGVSLSSVAPLPKKFYPLITSVDARFSNVTEYHAQFCDEGTLDPIKVKGKILICLSGE 431
H KG SLSS A KFYP++ SV+A+ N + AQ C G+LDPIK KGKIL+CL G+
Sbjct: 370 HYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQ 429
Query: 432 IEGIDKGFQAARAGAVGVVVANDLEVGNEIYPELHFLPASDITSNDNQLLQQYLNSTKTP 491
++KG A G +G+V+ N GN++ + H LPA+ +TS D+ + +Y++ TK P
Sbjct: 430 NGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKP 489
Query: 492 VAHLKKVITQLGVKPAPVMASFSSRGPNPIESSILKPDITAPGVNILASYPTGKAPTSSI 551
+AH+ T LG+KPAPVMASFSS+GP+ + ILKPDITAPGV+++A+Y +PT+
Sbjct: 490 IAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQ 549
Query: 552 SDRRRIPFNVISGTSMSCPHISGIAALLKSIHPEWSPAAIKSAIVTTAKTRGNNLQTILD 611
D RR+ FN ISGTSMSCPHISGIA LLK+ +P WSPAAI+SAI+TTA + I +
Sbjct: 550 FDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQN 609
Query: 612 SNKLRASPFAYGAGLVNPNNAMDPGLVYDTTVDDYLNFLCARGYNATQIRQFDTKPFICS 671
+ ++A+PF++GAG V PN A++PGLVYD + DYLNFLC+ GYNA+QI F F CS
Sbjct: 610 ATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCS 669
Query: 672 K-SFKLTDLNYPSISVGELKIGAPVTINRRVKNVGNPGTYVARVKVSPGVAVSVEPSTLQ 731
L +LNYPSI+V L + VT++R VKNVG P Y +V GV V+V+P++L
Sbjct: 670 SPKISLVNLNYPSITVPNL-TSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLN 729
Query: 732 FSSVGEEKAFKVVLQNTGKVTRNGYVFGTLIWSDGKHFVRSPMAIDL 776
F+ VGE+K FKV+L + GYVFG L+WSD KH VRSP+ + L
Sbjct: 730 FTKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772
BLAST of Lcy11g016530 vs. TAIR 10
Match:
AT5G59810.1 (Subtilase family protein )
HSP 1 Score: 813.5 bits (2100), Expect = 1.4e-235
Identity = 429/774 (55.43%), Postives = 529/774 (68.35%), Query Frame = 0
Query: 5 SNNKLPPLLLLFFFLISLLQTSTIATEKSYIVYLGSQLSSSLGPSALSEQSRQVTASHYD 64
SN L L++L + A +KSYIVYLGS P S V SH
Sbjct: 13 SNKMSLQSLSSLLLLVTLFFSPAFALKKSYIVYLGSHAHL---PQISSAHLDGVAHSHRT 72
Query: 65 LLGSMLGSKTMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPKVISVFENKPRKLHTTR 124
L S +GS A+EAIFYSY R NGFAA LDE EAA++A++P V+SVF NK RKLHTT
Sbjct: 73 FLASFVGSHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTH 132
Query: 125 SWNFLGLENDAGIPSNSIWNAAKFGQDVILANVDTGVWPESQSFSDEGYGPVPSKWRGIC 184
SWNF+ L + + +S+WN A +G+D I+AN+DTGVWPES+SFSDEGYG VP++W+G C
Sbjct: 133 SWNFMLLAKNGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRC 192
Query: 185 QSDSTFHCNKKLIGARYFYKGYEAAGGV-LNASLLTVRDHDGHGTHTLSTAAGNFVTGAN 244
D CN+KLIGARYF KGY A G+ NAS T RDHDGHG+HTLSTAAGNFV GAN
Sbjct: 193 HKD--VPCNRKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGAN 252
Query: 245 VFGHGNGTAKGGAPKARVAAYKVCWPSFMDGQCFDADTLAAFEAAIADGVDVISVSLGGT 304
VFG GNGTA GG+PKARVAAYKVCWP +CFDAD LAA EAAI DGVDV+S S+GG
Sbjct: 253 VFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGD 312
Query: 305 ANEFVNDPLSIVAFHAVQQGIVVVFSAGNSGPFPGTVTNVAPWQITVAASTDDRDFASYV 364
A ++++D ++I +FHAV+ G+ VV SAGNSGP GTV+NVAPW ITV AS+ DR+F ++V
Sbjct: 313 AGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFV 372
Query: 365 ALGNKKHLKGVSLSSVAPLP-KKFYPLITSVDARFSNVTEYHAQFCDEGTLDPIKVKGKI 424
L N + KG SLS PLP +K Y LI++ DA +N A C +G+LDP KVKGKI
Sbjct: 373 ELKNGQSFKGTSLSK--PLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKI 432
Query: 425 LICLSGEIEGIDKGFQAARAGAVGVVVANDLEVGNEIYPELHFLPASDITSNDNQLLQQY 484
L+CL G+ +DKG QAA AGA G+V+ ND GNEI + H LPAS I D + L Y
Sbjct: 433 LVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSY 492
Query: 485 LNSTKTPVAHLKKVITQLGVKPAPVMASFSSRGPNPIESSILKPDITAPGVNILASYPTG 544
L+STK P ++K L KPAP MASFSSRGPN I ILKPDITAPGVNI+A++
Sbjct: 493 LSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEA 552
Query: 545 KAPTSSISDRRRIPFNVISGTSMSCPHISGIAALLKSIHPEWSPAAIKSAIVTTAKTRGN 604
PT SD RR PFN SGTSMSCPHISG+ LLK++HP WSPAAI+SAI+TT++TR N
Sbjct: 553 TGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNN 612
Query: 605 NLQTILDSNKLRASPFAYGAGLVNPNNAMDPGLVYDTTVDDYLNFLCARGYNATQIRQFD 664
+ ++D + +A+PF+YG+G V PN A PGLVYD T DYL+FLCA GYN T ++ F
Sbjct: 613 RRKPMVDESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFA 672
Query: 665 TKP-FICSKSFKLTDLNYPSISVGELKIGAPVTINRRVKNVGNPGTYVARVKVSPGVAVS 724
P + C + L D NYPSI+V L +T+ R++KNVG P TY AR + GV VS
Sbjct: 673 EDPQYTCRQGANLLDFNYPSITVPNLT--GSITVTRKLKNVGPPATYNARFREPLGVRVS 732
Query: 725 VEPSTLQFSSVGEEKAFKVVLQNTGKVTRNGYVFGTLIWSDGKHFVRSPMAIDL 776
VEP L F+ GE K F++ L+ VT +GYVFG L W+D H+VRSP+ + L
Sbjct: 733 VEPKQLTFNKTGEVKIFQMTLRPL-PVTPSGYVFGELTWTDSHHYVRSPIVVQL 776
BLAST of Lcy11g016530 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 601.3 bits (1549), Expect = 1.1e-171
Identity = 344/771 (44.62%), Postives = 481/771 (62.39%), Query Frame = 0
Query: 16 FFFLISL---LQTSTIATEKSYIVYLG-SQLSSSLGPSALSEQSRQVTASHYDLLGSMLG 75
FF L+ L +S+ + + +YIV++ SQ+ SS + ++ YD S L
Sbjct: 11 FFLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSF----------DLHSNWYD---SSLR 70
Query: 76 SKTMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPKVISVFENKPRKLHTTRSWNFLGL 135
S + + E + Y+Y + +GF+ +L ++EA L P VISV +LHTTR+ FLGL
Sbjct: 71 SISDSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGL 130
Query: 136 ENDAGIPSNSIWNAAKFGQDVILANVDTGVWPESQSFSDEGYGPVPSKWRGICQSDSTFH 195
+ ++ A DV++ +DTGVWPES+S+SDEG+GP+PS W+G C++ + F
Sbjct: 131 DEHTA----DLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFT 190
Query: 196 ---CNKKLIGARYFYKGYEAAGGVLNAS--LLTVRDHDGHGTHTLSTAAGNFVTGANVFG 255
CN+KLIGAR+F +GYE+ G ++ S + RD DGHGTHT STAAG+ V GA++ G
Sbjct: 191 ASLCNRKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLG 250
Query: 256 HGNGTAKGGAPKARVAAYKVCWPSFMDGQCFDADTLAAFEAAIADGVDVISVSLGGTANE 315
+ +GTA+G AP+ARVA YKVCW G CF +D LAA + AIAD V+V+S+SLGG ++
Sbjct: 251 YASGTARGMAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSD 310
Query: 316 FVNDPLSIVAFHAVQQGIVVVFSAGNSGPFPGTVTNVAPWQITVAASTDDRDFASYVALG 375
+ D ++I AF A+++GI+V SAGN+GP +++NVAPW TV A T DRDF + LG
Sbjct: 311 YYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILG 370
Query: 376 NKKHLKGVSLSSVAPLPKKFYPLITSVDARFSNVTEYHAQFCDEGTLDPIKVKGKILICL 435
N K+ GVSL LP K P I + +A SN T + C GTL P KVKGKI++C
Sbjct: 371 NGKNFTGVSLFKGEALPDKLLPFIYAGNA--SNAT--NGNLCMTGTLIPEKVKGKIVMCD 430
Query: 436 SGEIEGIDKGFQAARAGAVGVVVANDLEVGNEIYPELHFLPASDITSNDNQLLQQYLNST 495
G + KG AG VG+++AN G E+ + H LPA+ + +++ Y+ +
Sbjct: 431 RGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTD 490
Query: 496 KTPVAHLKKVITQLGVKPAPVMASFSSRGPNPIESSILKPDITAPGVNILASYPTGKAPT 555
P A + + T +GVKP+PV+A+FSSRGPN I +ILKPD+ APGVNILA++ PT
Sbjct: 491 PNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPT 550
Query: 556 SSISDRRRIPFNVISGTSMSCPHISGIAALLKSIHPEWSPAAIKSAIVTTAKTRGNNLQT 615
SD RR+ FN+ISGTSMSCPH+SG+AALLKS+HPEWSPAAI+SA++TTA + +
Sbjct: 551 GLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKP 610
Query: 616 ILD-SNKLRASPFAYGAGLVNPNNAMDPGLVYDTTVDDYLNFLCARGYNATQIRQFDTKP 675
+LD + ++PF +GAG V+P A +PGL+YD T +DYL FLCA Y + QIR +
Sbjct: 611 LLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRN 670
Query: 676 FIC--SKSFKLTDLNYPSISVGELKIGAPVTINRRVKNVGNPGTYVARV-KVSPGVAVSV 735
+ C SKS+ + DLNYPS +V +GA R V +VG GTY +V + GV +SV
Sbjct: 671 YTCDPSKSYSVADLNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTYSVKVTSETTGVKISV 730
Query: 736 EPSTLQFSSVGEEKAFKVVLQNTGKVTRNGYVFGTLIWSDGKHFVRSPMAI 774
EP+ L F E+K++ V FG++ WSDGKH V SP+AI
Sbjct: 731 EPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754
BLAST of Lcy11g016530 vs. TAIR 10
Match:
AT4G34980.1 (subtilisin-like serine protease 2 )
HSP 1 Score: 575.5 bits (1482), Expect = 6.5e-164
Identity = 323/723 (44.67%), Postives = 452/723 (62.52%), Query Frame = 0
Query: 72 SKTMAQEA-IFYSYTRSFNGFAAKLDEKEAADLARNPKVISVFENKPRKLHTTRSWNFLG 131
S A+E+ I + Y F+GF+A + EA +L +P V++VFE++ R+LHTTRS FLG
Sbjct: 49 STEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLG 108
Query: 132 LENDAGIPSNSIWNAAKFGQDVILANVDTGVWPESQSFSDEGYGPVPSKWRGICQSDSTF 191
L+N G +W+ + +G DVI+ DTG+WPE +SFSD GP+P +WRG+C+S + F
Sbjct: 109 LQNQKG-----LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARF 168
Query: 192 ---HCNKKLIGARYFYKGYEAA--GGV-LNASLLTVRDHDGHGTHTLSTAAGNFVTGANV 251
+CN+K+IGAR+F KG +AA GG+ L+ RD DGHGTHT STAAG A++
Sbjct: 169 SPRNCNRKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASM 228
Query: 252 FGHGNGTAKGGAPKARVAAYKVCWPSFMDGQCFDADTLAAFEAAIADGVDVISVSLG--- 311
G+ +G AKG APKAR+AAYKVCW D C D+D LAAF+AA+ DGVDVIS+S+G
Sbjct: 229 SGYASGVAKGVAPKARIAAYKVCW---KDSGCLDSDILAAFDAAVRDGVDVISISIGGGD 288
Query: 312 GTANEFVNDPLSIVAFHAVQQGIVVVFSAGNSGPFPGTVTNVAPWQITVAASTDDRDFAS 371
G + + DP++I ++ A +GI V SAGN GP +VTN+APW TV AST DR+F +
Sbjct: 289 GITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPA 348
Query: 372 YVALGNKKHLKGVSLSSVAPLPKKFYPLITSVDARFSNVTEYHAQFCDEGTLDPIKVKGK 431
LG+ L+GVSL + PL + +P++ + S+ A C E TLDP +V+GK
Sbjct: 349 DAILGDGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSS-----ASLCMENTLDPKQVRGK 408
Query: 432 ILICLSGEIEGIDKGFQAARAGAVGVVVANDLEVGNEIYPELHFLPASDITSNDNQLLQQ 491
I+IC G + KG +AG VG+++AN G + + H +PA + SN+ ++
Sbjct: 409 IVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKA 468
Query: 492 YLNSTKTPVAHLKKVITQLGVKPAPVMASFSSRGPNPIESSILKPDITAPGVNILASYPT 551
Y +S P+A + T +G+KPAPV+ASFS RGPN + ILKPD+ APGVNILA++
Sbjct: 469 YASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTD 528
Query: 552 GKAPTSSISDRRRIPFNVISGTSMSCPHISGIAALLKSIHPEWSPAAIKSAIVTTAKTRG 611
PT SD R+ FN++SGTSM+CPH+SG AALLKS HP+WSPA I+SA++TT
Sbjct: 529 AVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVD 588
Query: 612 NNLQTILD-SNKLRASPFAYGAGLVNPNNAMDPGLVYDTTVDDYLNFLCARGYNATQIRQ 671
N+ ++++D S A+P+ YG+G +N AM+PGLVYD T DDY+ FLC+ GY I+
Sbjct: 589 NSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQV 648
Query: 672 FDTKPFICSKSFKLT--DLNYPSISV---GELKIGAPVTINRRVKNVGN-PGTYVARVKV 731
P C + K + +LNYPSI+ + T+ R NVG Y AR++
Sbjct: 649 ITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIES 708
Query: 732 SPGVAVSVEPSTLQFSSVGEEKAFKV-VLQNTGKVT--RNGYVFGTLIWSD-GKHFVRSP 774
GV V+V+P L F+S + +++ V V NT V G VFG++ W D GKH VRSP
Sbjct: 709 PRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSP 758
BLAST of Lcy11g016530 vs. TAIR 10
Match:
AT3G14240.1 (Subtilase family protein )
HSP 1 Score: 574.3 bits (1479), Expect = 1.5e-163
Identity = 338/791 (42.73%), Postives = 468/791 (59.17%), Query Frame = 0
Query: 16 FFFLISLLQTSTIATEK---SYIVYLGSQLSSSLGPSALSEQSRQVTASHYDLLGSMLGS 75
FFFL++L S+ A+ +YIV++ + S+ P +H+ S L S
Sbjct: 7 FFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFP------------THFHWYTSSLAS 66
Query: 76 KTMAQEAIFYSYTRSFNGFAAKLDEKEAADLARNPKVISVFENKPRKLHTTRSWNFLGLE 135
T + +I ++Y F+GF+A+L ++A+ L +P VISV + R LHTTRS FLGL
Sbjct: 67 LTSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLR 126
Query: 136 --NDAGIPSNSIWNAAKFGQDVILANVDTGVWPESQSFSDEGYGPVPSKWRGICQSDSTF 195
+ AG+ S FG D+++ +DTGVWPE SF D G GPVP KW+G C + F
Sbjct: 127 STDKAGLLEES-----DFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDF 186
Query: 196 ---HCNKKLIGARYFYKGYEAAGGVLN--ASLLTVRDHDGHGTHTLSTAAGNFVTGANVF 255
CN+KL+GAR+F GYEA G +N + RD DGHGTHT S +AG +V A+
Sbjct: 187 PESACNRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTL 246
Query: 256 GHGNGTAKGGAPKARVAAYKVCWPSFMDGQCFDADTLAAFEAAIADGVDVISVSLGGTAN 315
G+ +G A G APKAR+AAYKVCW S C+D+D LAAF+ A+ADGVDVIS+S+GG
Sbjct: 247 GYAHGVAAGMAPKARLAAYKVCWNS----GCYDSDILAAFDTAVADGVDVISLSVGGVVV 306
Query: 316 EFVNDPLSIVAFHAVQQGIVVVFSAGNSGPFPGTVTNVAPWQITVAASTDDRDFASYVAL 375
+ D ++I AF A+ +GI V SAGN GP TVTNVAPW TV A T DRDF + V L
Sbjct: 307 PYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKL 366
Query: 376 GNKKHLKGVSLSSVAPL-PKKFYPLITSVDARFSNVTEYHAQFCDEGTLDPIKVKGKILI 435
GN K + GVS+ L P + YPL+ Y + C EG+LDP VKGKI++
Sbjct: 367 GNGKMISGVSVYGGPGLDPGRMYPLV--YGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVL 426
Query: 436 CLSGEIEGIDKGFQAARAGAVGVVVANDLEVGNEIYPELHFLPASDITSNDNQLLQQYLN 495
C G KG + G +G+++AN + G + + H LPA+ + ++ +++Y++
Sbjct: 427 CDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYIS 486
Query: 496 ------STKTPVAHLKKVITQLGVKPAPVMASFSSRGPNPIESSILKPDITAPGVNILAS 555
S+K P A + T+LG++PAPV+ASFS+RGPNP ILKPD+ APG+NILA+
Sbjct: 487 ESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAA 546
Query: 556 YPTGKAPTSSISDRRRIPFNVISGTSMSCPHISGIAALLKSIHPEWSPAAIKSAIVTTAK 615
+P P+ SD RR FN++SGTSM+CPH+SG+AALLK+ HP+WSPAAI+SA++TTA
Sbjct: 547 WPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAY 606
Query: 616 TRGNNLQTILD-SNKLRASPFAYGAGLVNPNNAMDPGLVYDTTVDDYLNFLCARGYNATQ 675
T N+ + ++D S +S YG+G V+P AMDPGLVYD T DY+NFLC Y T
Sbjct: 607 TVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTN 666
Query: 676 IRQFDTKPFICS---KSFKLTDLNYPSISV-----GELKIGAPVTINRRVKNVG-NPGTY 735
I + C ++ + +LNYPS SV GE K+ R V NVG + Y
Sbjct: 667 IVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFI--RTVTNVGDSDSVY 726
Query: 736 VARVKVSPGVAVSVEPSTLQFSSVGEEKAFKVVLQNTGKVTRNGYV---FGTLIWSDGKH 777
+++ G V+VEP L F VG++ +F V ++ T G G ++WSDGK
Sbjct: 727 EIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKR 772
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q9ZSP5 | 4.2e-240 | 55.28 | Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... | [more] |
| F4JXC5 | 2.0e-234 | 55.43 | Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... | [more] |
| I1N462 | 1.2e-207 | 51.15 | Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... | [more] |
| O65351 | 1.6e-170 | 44.62 | Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... | [more] |
| O49607 | 9.2e-163 | 44.67 | Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A1S4DX90 | 0.0e+00 | 80.75 | subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491001 PE=3 SV=... | [more] |
| A0A0A0L241 | 0.0e+00 | 79.84 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045090 PE=3 SV=1 | [more] |
| A0A5D3D763 | 0.0e+00 | 76.65 | Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... | [more] |
| A0A6J1CQS4 | 0.0e+00 | 74.61 | subtilisin-like protease SBT5.3 OS=Momordica charantia OX=3673 GN=LOC111013337 P... | [more] |
| A0A6J1L3L8 | 1.1e-307 | 68.64 | subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111498821 PE=3... | [more] |
