Lcy11g016400 (gene) Sponge gourd (P93075) v1

BLAST of Lcy11g016400 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 857.1 bits (2213), Expect = 1.5e-247
Identity = 424/706 (60.06%), Postives = 527/706 (74.65%), Query Frame = 0

Query: 16  QTAKEAIFYSYNRHINGFAAMLDQKAAEDLAKHPAVASINENKGRKLHTTSSWNFLGVEH 75
           + A +AIFYSY +HINGFAA LD   A +++KHP V S+  NK  KLHTT SW+FLG+EH
Sbjct: 69  ERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEH 128

Query: 76  DDGIPANSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPIPTRWRGSCEG--GSKFH 135
           +  +P++SIW  A FGE TII NLDTGVWPESKSF DEG GPIP+RW+G C+    + FH
Sbjct: 129 NSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFH 188

Query: 136 CNSRKLIGARYFNKGYAAYVGSLNASFETARDHDGHGTHTLSTAGGNFISGASVFGNGNG 195
           CN RKLIGARYFNKGYAA VG LN+SF++ RD DGHG+HTLSTA G+F+ G S+FG GNG
Sbjct: 189 CN-RKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNG 248

Query: 196 TAKGGSPKARVVAYKVCWPKIDSSGGCFDADILAAIEAAITDGVDVLSISLGGDSKDFFD 255
           TAKGGSP+ARV AYKVCWP +     C+DAD+LAA +AAI DG DV+S+SLGG+   FF+
Sbjct: 249 TAKGGSPRARVAAYKVCWPPV-KGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFN 308

Query: 256 DVNAIGAFHAVQHGIIVVCSAGNSGPAPGTVENVAPWLVTVGASTINRQFTSYVALGNKT 315
           D  AIG+FHA +  I+VVCSAGNSGPA  TV NVAPW +TVGAST++R+F S + LGN  
Sbjct: 309 DSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGK 368

Query: 316 HIKGASLSDKILPSQKFYPLINSVDAKASSVSNNNAQLCEEGSLDPKKVKGKILVCLRGE 375
           H KG SLS   LP  KFYP++ SV+AKA + S  +AQLC+ GSLDP K KGKILVCLRG+
Sbjct: 369 HYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQ 428

Query: 376 NGRVDKGFVAAQAGAVGMILANSEQNGDELTADAHLLPASHVSYTDGRSVYQYINSTKIP 435
           NGRV+KG   A  G +GM+L N+   G++L AD H+LPA+ ++  D  +V +YI+ TK P
Sbjct: 429 NGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKP 488

Query: 436 MAFMTRVRTEVGVKPAPFMAEFSSRGPNEIEASILKPDITAPGVSIIAAFAGDLSPTGED 495
           +A +T  RT++G+KPAP MA FSS+GP+ +   ILKPDITAPGVS+IAA+ G +SPT E 
Sbjct: 489 IAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQ 548

Query: 496 LDTRRIPYMAESGTSMSCPHVSGIVGLLRTLYPKWSPAAIRSAIMTTAETKANDLNPILS 555
            D RR+ + A SGTSMSCPH+SGI GLL+T YP WSPAAIRSAIMTTA    +   PI +
Sbjct: 549 FDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQN 608

Query: 556 SSQVKATPFAYGAGHVNPNRAANPGLVYDLTTKDYLNFLCAHGYNETQLKQFSNATFACS 615
           ++ +KATPF++GAGHV PN A NPGLVYDL  KDYLNFLC+ GYN +Q+  FS   F CS
Sbjct: 609 ATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCS 668

Query: 616 K-TFKVTDLNYPSISIPDLKVGTAKVKRRVKNVGSPGTYVAQVKAPPGVSVSVEPSSLKF 675
                + +LNYPSI++P+L      V R VKNVG P  Y  +V  P GV V+V+P+SL F
Sbjct: 669 SPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNF 728

Query: 676 TGVGEEKSFRVVLKRVSNDYHRGYVFGRLAWTDGKHRVRSPIVVNL 719
           T VGE+K+F+V+L +   +  +GYVFG L W+D KHRVRSPIVV L
Sbjct: 729 TKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of Lcy11g016400 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 847.4 bits (2188), Expect = 1.2e-244
Identity = 428/705 (60.71%), Postives = 524/705 (74.33%), Query Frame = 0

Query: 16  QTAKEAIFYSYNRHINGFAAMLDQKAAEDLAKHPAVASINENKGRKLHTTSSWNFLGVEH 75
           + AKEAIFYSY RHINGFAA+LD+  A ++AKHP V S+  NKGRKLHTT SWNF+ +  
Sbjct: 79  ENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAK 138

Query: 76  DDGIPANSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPIPTRWRGSCEGGSKFHCN 135
           +  +  +S+WN A +GE TII NLDTGVWPESKSFSDEGYG +P RW+G C       CN
Sbjct: 139 NGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVPCN 198

Query: 136 SRKLIGARYFNKGYAAYVG-SLNASFETARDHDGHGTHTLSTAGGNFISGASVFGNGNGT 195
            RKLIGARYFNKGY AY G   NAS+ET RDHDGHG+HTLSTA GNF+ GA+VFG GNGT
Sbjct: 199 -RKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGT 258

Query: 196 AKGGSPKARVVAYKVCWPKIDSSGGCFDADILAAIEAAITDGVDVLSISLGGDSKDFFDD 255
           A GGSPKARV AYKVCWP +D +  CFDADILAAIEAAI DGVDVLS S+GGD+ D+  D
Sbjct: 259 ASGGSPKARVAAYKVCWPPVDGA-ECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSD 318

Query: 256 VNAIGAFHAVQHGIIVVCSAGNSGPAPGTVENVAPWLVTVGASTINRQFTSYVALGNKTH 315
             AIG+FHAV++G+ VVCSAGNSGP  GTV NVAPW++TVGAS+++R+F ++V L N   
Sbjct: 319 GIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQS 378

Query: 316 IKGASLSDKILPSQKFYPLINSVDAKASSVSNNNAQLCEEGSLDPKKVKGKILVCLRGEN 375
            KG SLS K LP +K Y LI++ DA  ++ +  +A LC++GSLDPKKVKGKILVCLRG+N
Sbjct: 379 FKGTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDN 438

Query: 376 GRVDKGFVAAQAGAVGMILANSEQNGDELTADAHLLPASHVSYTDGRSVYQYINSTKIPM 435
            RVDKG  AA AGA GM+L N + +G+E+ +DAH+LPAS + Y DG +++ Y++STK P 
Sbjct: 439 ARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPK 498

Query: 436 AFMTRVRTEVGVKPAPFMAEFSSRGPNEIEASILKPDITAPGVSIIAAFAGDLSPTGEDL 495
            ++      +  KPAPFMA FSSRGPN I   ILKPDITAPGV+IIAAF     PT  D 
Sbjct: 499 GYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDS 558

Query: 496 DTRRIPYMAESGTSMSCPHVSGIVGLLRTLYPKWSPAAIRSAIMTTAETKANDLNPILSS 555
           D RR P+  ESGTSMSCPH+SG+VGLL+TL+P WSPAAIRSAIMTT+ T+ N   P++  
Sbjct: 559 DNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDE 618

Query: 556 SQVKATPFAYGAGHVNPNRAANPGLVYDLTTKDYLNFLCAHGYNETQLKQFS-NATFACS 615
           S  KA PF+YG+GHV PN+AA+PGLVYDLTT DYL+FLCA GYN T ++ F+ +  + C 
Sbjct: 619 SFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCR 678

Query: 616 KTFKVTDLNYPSISIPDLKVGTAKVKRRVKNVGSPGTYVAQVKAPPGVSVSVEPSSLKFT 675
           +   + D NYPSI++P+L  G+  V R++KNVG P TY A+ + P GV VSVEP  L F 
Sbjct: 679 QGANLLDFNYPSITVPNL-TGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFN 738

Query: 676 GVGEEKSFRVVLKRVSNDYHRGYVFGRLAWTDGKHRVRSPIVVNL 719
             GE K F++ L+ +      GYVFG L WTD  H VRSPIVV L
Sbjct: 739 KTGEVKIFQMTLRPLPVT-PSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of Lcy11g016400 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 684.9 bits (1766), Expect = 1.0e-195
Identity = 379/725 (52.28%), Postives = 484/725 (66.76%), Query Frame = 0

Query: 16  QTAKEAIFYSYNRHINGFAAMLDQKAAEDLAKHPAVASINENKGRKLHTTSSWNFLGVEH 75
           + AKEAI YSYNRHINGFAA+L+++ A D+AK+P V S+  +K  KLHTT SW FLG+ H
Sbjct: 69  EKAKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGL-H 128

Query: 76  DDGIPANSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPIPTRWRGS-CE-----GG 135
             G   NS W    FGE+TIIGN+DTGVWPES+SFSD+GYG +P++WRG  C+     G 
Sbjct: 129 RRG--QNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKLPGS 188

Query: 136 SKFHCNSRKLIGARYFNKGYAAYVGSLNASFETARDHDGHGTHTLSTAGGNFISGASVFG 195
            K  CN RKLIGARY+NK + A+ G L+    TARD  GHGTHTLSTAGGNF+ GA VF 
Sbjct: 189 MKNTCN-RKLIGARYYNKAFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFA 248

Query: 196 NGNGTAKGGSPKARVVAYKVCWPKIDSSGGCFDADILAAIEAAITDGVDVLSISLGGD-- 255
            GNGTAKGGSP+ARV AYKVCW   D +  C+ AD+LAAI+ AI DGVDV+++S G    
Sbjct: 249 VGNGTAKGGSPRARVAAYKVCWSLTDPA-SCYGADVLAAIDQAIDDGVDVINVSFGVSYV 308

Query: 256 --SKDFFDDVNAIGAFHAVQHGIIVVCSAGNSGPAPGTVENVAPWLVTVGASTINRQFTS 315
             ++  F D  +IGAFHA+   I++V SAGN GP PGTV NVAPW+ T+ AST++R F+S
Sbjct: 309 VTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSS 368

Query: 316 YVALGNKTHIKGASLSDKILPSQKFYPLINSVDAKASSVSNNNAQLCEEGSLDPKKVKGK 375
            + + N+  I+GASL   + P+Q F  LI S DAK ++ +  +AQLC  G+LD  KV GK
Sbjct: 369 NLTINNQL-IEGASLFVNLPPNQAF-SLILSTDAKLANATFRDAQLCRRGTLDRTKVNGK 428

Query: 376 ILVCLR-GENGRVDKGFVAAQAGAVGMILANSEQNGDELTADAHLLPASHVSYTDGRSVY 435
           I++C R G+   V +G  A  AGA GMIL N  QNG  L+A+ H+    +      +S  
Sbjct: 429 IVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRP 488

Query: 436 QYINSTKI-----PMAF-----MTRVRTEVGVKPAPFMAEFSSRGPNEIEASILKPDITA 495
             + +T I     P+       M+R RT  G KPAP MA FSSRGPN+I+ SILKPD+TA
Sbjct: 489 HGVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTA 548

Query: 496 PGVSIIAAFAGDLSPTGEDLDTRR-IPYMAESGTSMSCPHVSGIVGLLRTLYPKWSPAAI 555
           PGV+I+AA++   S +   +D RR   +    GTSMSCPH SGI GLL+T +P WSPAAI
Sbjct: 549 PGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAI 608

Query: 556 RSAIMTTAETKANDLNPILSS-SQVKATPFAYGAGHVNPNRAANPGLVYDLTTKDYLNFL 615
           +SAIMTTA T  N   PI  +  +  A  FAYG+GHV P+ A  PGLVYDL+  DYLNFL
Sbjct: 609 KSAIMTTATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFL 668

Query: 616 CAHGYNETQLKQFS-NATFACSKTFKVTDLNYPSISIPDLKVGTAKVKRRVKNVGSPGTY 675
           CA GY++  +   + N TF CS +  V DLNYPSI++P+L++    + R V NVG P TY
Sbjct: 669 CASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNVGPPSTY 728

Query: 676 VAQVKAPPGVSVSVEPSSLKFTGVGEEKSFRVVLKRVSNDYHRGYVFGRLAWTDGKHRVR 717
               ++P G S++V P SL FT +GE K+F+V+++  S    R Y FG L WTDGKH VR
Sbjct: 729 TVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIVR 786

BLAST of Lcy11g016400 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 625.2 bits (1611), Expect = 9.4e-178
Identity = 339/704 (48.15%), Postives = 453/704 (64.35%), Query Frame = 0

Query: 22  IFYSYNRHINGFAAMLDQKAAEDLAKHPAVASINENKGRKLHTTSSWNFLGVEHDDGIPA 81
           + Y+Y   I+GF+  L Q+ A+ L   P V S+      +LHTT +  FLG++       
Sbjct: 65  LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTA--- 124

Query: 82  NSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPIPTRWRGSCEGGSKFHCN--SRKL 141
             ++  A      ++G LDTGVWPESKS+SDEG+GPIP+ W+G CE G+ F  +  +RKL
Sbjct: 125 -DLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKL 184

Query: 142 IGARYFNKGYAAYVGSLNASFE--TARDHDGHGTHTLSTAGGNFISGASVFGNGNGTAKG 201
           IGAR+F +GY + +G ++ S E  + RD DGHGTHT STA G+ + GAS+ G  +GTA+G
Sbjct: 185 IGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARG 244

Query: 202 GSPKARVVAYKVCWPKIDSSGGCFDADILAAIEAAITDGVDVLSISLGGDSKDFFDDVNA 261
            +P+ARV  YKVCW      GGCF +DILAAI+ AI D V+VLS+SLGG   D++ D  A
Sbjct: 245 MAPRARVAVYKVCW-----LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVA 304

Query: 262 IGAFHAVQHGIIVVCSAGNSGPAPGTVENVAPWLVTVGASTINRQFTSYVALGNKTHIKG 321
           IGAF A++ GI+V CSAGN+GP+  ++ NVAPW+ TVGA T++R F +   LGN  +  G
Sbjct: 305 IGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTG 364

Query: 322 ASLSDKILPSQKFYPLINSVDAKASSVSNNNAQLCEEGSLDPKKVKGKILVCLRGENGRV 381
            SL        K  P I +    AS+ +N N  LC  G+L P+KVKGKI++C RG N RV
Sbjct: 365 VSLFKGEALPDKLLPFIYA--GNASNATNGN--LCMTGTLIPEKVKGKIVMCDRGINARV 424

Query: 382 DKGFVAAQAGAVGMILANSEQNGDELTADAHLLPASHVSYTDGRSVYQYINSTKIPMAFM 441
            KG V   AG VGMILAN+  NG+EL ADAHLLPA+ V    G  +  Y+ +   P A +
Sbjct: 425 QKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASI 484

Query: 442 TRVRTEVGVKPAPFMAEFSSRGPNEIEASILKPDITAPGVSIIAAFAGDLSPTGEDLDTR 501
           + + T VGVKP+P +A FSSRGPN I  +ILKPD+ APGV+I+AA+ G   PTG   D+R
Sbjct: 485 SILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSR 544

Query: 502 RIPYMAESGTSMSCPHVSGIVGLLRTLYPKWSPAAIRSAIMTTAETKANDLNPILSSSQV 561
           R+ +   SGTSMSCPHVSG+  LL++++P+WSPAAIRSA+MTTA     D  P+L  +  
Sbjct: 545 RVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATG 604

Query: 562 K-ATPFAYGAGHVNPNRAANPGLVYDLTTKDYLNFLCAHGYNETQLKQFSNATFAC--SK 621
           K +TPF +GAGHV+P  A NPGL+YDLTT+DYL FLCA  Y   Q++  S   + C  SK
Sbjct: 605 KPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSK 664

Query: 622 TFKVTDLNYPSISIPDLKVGTAKVKRRVKNVGSPGTYVAQVKA-PPGVSVSVEPSSLKFT 681
           ++ V DLNYPS ++    VG  K  R V +VG  GTY  +V +   GV +SVEP+ L F 
Sbjct: 665 SYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFK 724

Query: 682 GVGEEKSFRVVLKRVSNDYHRGYVFGRLAWTDGKHRVRSPIVVN 718
              E+KS+ V     S+       FG + W+DGKH V SP+ ++
Sbjct: 725 EANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of Lcy11g016400 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 8.0e-161
Identity = 327/726 (45.04%), Postives = 451/726 (62.12%), Query Frame = 0

Query: 13  HKTQTAKEA-IFYSYNRHINGFAAMLDQKAAEDLAKHPAVASINENKGRKLHTTSSWNFL 72
           + T+ A+E+ I + Y+   +GF+A++    A++L  HPAV ++ E++ R+LHTT S  FL
Sbjct: 48  YSTEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFL 107

Query: 73  GVEHDDGIPANSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPIPTRWRGSCEGGSK 132
           G+++  G     +W+ + +G   IIG  DTG+WPE +SFSD   GPIP RWRG CE G++
Sbjct: 108 GLQNQKG-----LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGAR 167

Query: 133 F---HCNSRKLIGARYFNKG-YAAYVGSLNASFE--TARDHDGHGTHTLSTAGGNFISGA 192
           F   +CN RK+IGAR+F KG  AA +G +N + E  + RD DGHGTHT STA G     A
Sbjct: 168 FSPRNCN-RKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKA 227

Query: 193 SVFGNGNGTAKGGSPKARVVAYKVCWPKIDSSGGCFDADILAAIEAAITDGVDVLSISLG 252
           S+ G  +G AKG +PKAR+ AYKVCW       GC D+DILAA +AA+ DGVDV+SIS+G
Sbjct: 228 SMSGYASGVAKGVAPKARIAAYKVCW----KDSGCLDSDILAAFDAAVRDGVDVISISIG 287

Query: 253 GD---SKDFFDDVNAIGAFHAVQHGIIVVCSAGNSGPAPGTVENVAPWLVTVGASTINRQ 312
           G    +  ++ D  AIG++ A   GI V  SAGN GP   +V N+APW+ TVGASTI+R 
Sbjct: 288 GGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRN 347

Query: 313 FTSYVALGNKTHIKGASLSDKILPSQKFYPLINSVDAKASSVSNNNAQLCEEGSLDPKKV 372
           F +   LG+   ++G SL   +  + + +P++    +  SS     A LC E +LDPK+V
Sbjct: 348 FPADAILGDGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSS-----ASLCMENTLDPKQV 407

Query: 373 KGKILVCLRGENGRVDKGFVAAQAGAVGMILANSEQNGDELTADAHLLPASHVSYTDGRS 432
           +GKI++C RG + RV KG V  +AG VGMILAN   NG+ L  DAHL+PA  V   +G  
Sbjct: 408 RGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDR 467

Query: 433 VYQYINSTKIPMAFMTRVRTEVGVKPAPFMAEFSSRGPNEIEASILKPDITAPGVSIIAA 492
           +  Y +S   P+A +    T VG+KPAP +A FS RGPN +   ILKPD+ APGV+I+AA
Sbjct: 468 IKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAA 527

Query: 493 FAGDLSPTGEDLDTRRIPYMAESGTSMSCPHVSGIVGLLRTLYPKWSPAAIRSAIMTTAE 552
           +   + PTG   D R+  +   SGTSM+CPHVSG   LL++ +P WSPA IRSA+MTT  
Sbjct: 528 WTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTN 587

Query: 553 TKANDLNPILSSSQVK-ATPFAYGAGHVNPNRAANPGLVYDLTTKDYLNFLCAHGYNETQ 612
              N    ++  S  K ATP+ YG+GH+N  RA NPGLVYD+T  DY+ FLC+ GY    
Sbjct: 588 LVDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKT 647

Query: 613 LKQFSNATFACSKTFKVT--DLNYPSIS--IPDLKVG--TAKVKRRVKNVG-SPGTYVAQ 672
           ++  +     C  T K +  +LNYPSI+   P  + G  +  V R   NVG +   Y A+
Sbjct: 648 IQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRAR 707

Query: 673 VKAPPGVSVSVEPSSLKFTGVGEEKSFRVVLKRVSNDY---HRGYVFGRLAWTD-GKHRV 717
           +++P GV+V+V+P  L FT   + +S+ V +   + +      G VFG + W D GKH V
Sbjct: 708 IESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVV 758

BLAST of Lcy11g016400 vs. ExPASy TrEMBL
Match: A0A1S4DX85 (subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491136 PE=3 SV=1)

HSP 1 Score: 1167.5 bits (3019), Expect = 0.0e+00
Identity = 563/704 (79.97%), Postives = 631/704 (89.63%), Query Frame = 0

Query: 16  QTAKEAIFYSYNRHINGFAAMLDQKAAEDLAKHPAVASINENKGRKLHTTSSWNFLGVEH 75
           + AKEAIFYSYNRHINGFAA+LDQK  EDLA++PAVASI+ENKGRKLHTTSSW FLGVEH
Sbjct: 45  EAAKEAIFYSYNRHINGFAAILDQKVVEDLARNPAVASIHENKGRKLHTTSSWKFLGVEH 104

Query: 76  DDGIPANSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPIPTRWRGSCEGGSKFHCN 135
           DDGIP NSIWN ASFGESTIIGNLDTGVWPESKSF+DEGYGP+PTRW+GSCEGGSKFHCN
Sbjct: 105 DDGIPHNSIWNRASFGESTIIGNLDTGVWPESKSFNDEGYGPVPTRWKGSCEGGSKFHCN 164

Query: 136 SRKLIGARYFNKGYAAYVGSLNASFETARDHDGHGTHTLSTAGGNFISGASVFGNGNGTA 195
            RKLIGARYFNKGYAAY GSLNAS+ETARD++GHGTHTLSTAGGNFISGA+VFGNGNGTA
Sbjct: 165 -RKLIGARYFNKGYAAYAGSLNASYETARDNEGHGTHTLSTAGGNFISGANVFGNGNGTA 224

Query: 196 KGGSPKARVVAYKVCWPKIDSSGGCFDADILAAIEAAITDGVDVLSISLGGDSKDFFDDV 255
           KGGSPKA V AYKVCWP++DS GGCFDADILAAIEAAI+DGVD+LS+SLGG +KDF +DV
Sbjct: 225 KGGSPKALVAAYKVCWPQVDSGGGCFDADILAAIEAAISDGVDILSLSLGGGAKDFSEDV 284

Query: 256 NAIGAFHAVQHGIIVVCSAGNSGPAPGTVENVAPWLVTVGASTINRQFTSYVALGNKTHI 315
            AIGAFHAVQ GIIVVCSAGNSGPAPGT+EN APW++TVGASTINR FTSYVALGNK HI
Sbjct: 285 TAIGAFHAVQQGIIVVCSAGNSGPAPGTIENGAPWILTVGASTINRDFTSYVALGNKKHI 344

Query: 316 KGASLSDKILPSQKFYPLINSVDAKASSVSNNNAQLCEEGSLDPKKVKGKILVCLRGENG 375
           KGASLSDKILP QKFYPLIN+ DAKA++VS++ AQLC+ GSLDPKKVKGKI++CLRGEN 
Sbjct: 345 KGASLSDKILPEQKFYPLINAADAKANNVSSDVAQLCQAGSLDPKKVKGKIILCLRGENA 404

Query: 376 RVDKGFVAAQAGAVGMILANSEQNGDELTADAHLLPASHVSYTDGRSVYQYINSTKIPMA 435
           RVDKG+ AAQAGAVGMILAN+EQNGDEL ADAHLLP SHVSYTDG+S+YQYIN TK PMA
Sbjct: 405 RVDKGYAAAQAGAVGMILANAEQNGDELIADAHLLPVSHVSYTDGQSIYQYINFTKTPMA 464

Query: 436 FMTRVRTEVGVKPAPFMAEFSSRGPNEIEASILKPDITAPGVSIIAAFAGDLSPTGEDLD 495
           +MT VRTE+G+KPAP MA FSSRGPN IE SILKPDITAPGV+I+AA++ D SP+G   D
Sbjct: 465 YMTHVRTELGIKPAPVMASFSSRGPNTIEESILKPDITAPGVNILAAYSEDASPSGSLFD 524

Query: 496 TRRIPYMAESGTSMSCPHVSGIVGLLRTLYPKWSPAAIRSAIMTTAETKANDLNPILSSS 555
            RRIP+   SGTSMSCPH+SGIVGLL+TLYP WSPAAI+SAIMTTAET+ANDL+PIL++ 
Sbjct: 525 NRRIPFNIVSGTSMSCPHISGIVGLLKTLYPTWSPAAIKSAIMTTAETRANDLHPILNTV 584

Query: 556 QVKATPFAYGAGHVNPNRAANPGLVYDLTTKDYLNFLCAHGYNETQLKQFSNATFACSKT 615
            +KA PFAYGAGHV PNRA NPGLVYDLTT DY+NFLCA GYN++Q+ +FS  +F CSK+
Sbjct: 585 NLKANPFAYGAGHVQPNRAMNPGLVYDLTTNDYMNFLCAQGYNKSQISKFSATSFVCSKS 644

Query: 616 FKVTDLNYPSISIPDLKVGTAKVKRRVKNVGSPGTYVAQVKAPPGVSVSVEPSSLKFTGV 675
           FK+TD NYPSISIPD+K G   + RRVKNVG P TYVA+VK P GVSVSVEP +LKFTG+
Sbjct: 645 FKLTDFNYPSISIPDMKSGVVTINRRVKNVGKPSTYVARVKVPQGVSVSVEPRTLKFTGI 704

Query: 676 GEEKSFRVVLKRVSNDYHRGYVFGRLAWTDGKHRVRSPIVVNLG 720
            EEKSF+VV+  V+N+ HRGYVFG L W DGKH VRSPIVVNLG
Sbjct: 705 DEEKSFKVVIGSVANNKHRGYVFGSLIWEDGKHHVRSPIVVNLG 747

BLAST of Lcy11g016400 vs. ExPASy TrEMBL
Match: A0A6J1CQB4 (subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111013335 PE=3 SV=1)

HSP 1 Score: 1123.6 bits (2905), Expect = 0.0e+00
Identity = 546/702 (77.78%), Postives = 622/702 (88.60%), Query Frame = 0

Query: 18  AKEAIFYSYNRHINGFAAMLDQKAAEDLAKHPAVASINENKGRKLHTTSSWNFLGVEHDD 77
           AK+AIFYSYN++INGFAA+LD+K A++LAKHP+V S++ENK RKLHTT SW+FLG+E+D 
Sbjct: 69  AKDAIFYSYNKYINGFAAILDEKVAQELAKHPSVVSVHENKARKLHTTGSWSFLGLENDG 128

Query: 78  GIPANSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPIPTRWRGSCEGGSKFHCNSR 137
            IP+NSIWNLASFGESTIIGNLDTGVWPESKSFSD+GYGPIP+RWRGSCEGGSKF CN R
Sbjct: 129 EIPSNSIWNLASFGESTIIGNLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSKFRCN-R 188

Query: 138 KLIGARYFNKGYAAYVGSLNASFETARDHDGHGTHTLSTAGGNFISGASVFGNGNGTAKG 197
           KLIGARYFNKGY A +G LNAS+ETARD +GHGTHTLSTAGGNF+SGASVFGNGNGTAKG
Sbjct: 189 KLIGARYFNKGYVASIGPLNASYETARDDEGHGTHTLSTAGGNFVSGASVFGNGNGTAKG 248

Query: 198 GSPKARVVAYKVCWPKIDSSGGCFDADILAAIEAAITDGVDVLSISLGGDSKDFFDDVNA 257
           GSP+ARV AY+VCWP +  +GGCF ADILA  EAAI DGVDVLS+SLGG  ++F DD  A
Sbjct: 249 GSPRARVAAYRVCWPAV-LTGGCFMADILAGFEAAIHDGVDVLSVSLGGSPEEFSDDGLA 308

Query: 258 IGAFHAVQHGIIVVCSAGNSGPAPGTVENVAPWLVTVGASTINRQFTSYVALGNKTHIKG 317
           IGAFHAVQHGI VVCSAGNSGP  GTV NVAPW++TVGAST +R F SYV LGN+ HIKG
Sbjct: 309 IGAFHAVQHGITVVCSAGNSGPGRGTVSNVAPWMITVGASTADRLFASYVGLGNRKHIKG 368

Query: 318 ASLSDKILPSQKFYPLINSVDAKASSVSNNNAQLCEEGSLDPKKVKGKILVCLRGENGRV 377
           ASLSDKILP+QKFYPLI++ DAKAS+VS   AQLCEEGSLDPKKV+GKI+VCLRG+N RV
Sbjct: 369 ASLSDKILPAQKFYPLISTADAKASNVSLEYAQLCEEGSLDPKKVEGKIVVCLRGDNARV 428

Query: 378 DKGFVAAQAGAVGMILANSEQNGDELTADAHLLPASHVSYTDGRSVYQYINSTKIPMAFM 437
           DKG+VAAQAGAVGMILAN++ NGDEL ADAHLLPASHVSY DG  ++QYI STKIPMA+M
Sbjct: 429 DKGYVAAQAGAVGMILANAQDNGDELLADAHLLPASHVSYADGELIFQYIKSTKIPMAYM 488

Query: 438 TRVRTEVGVKPAPFMAEFSSRGPNEIEASILKPDITAPGVSIIAAFAGDLSPTGEDLDTR 497
           T V+TE+GVKPAPFMA FSSRGPN IE SILKPDI APGVSIIAA++ + SP+G   D R
Sbjct: 489 THVKTELGVKPAPFMASFSSRGPNTIEESILKPDIIAPGVSIIAAYSEEASPSGSSFDKR 548

Query: 498 RIPYMAESGTSMSCPHVSGIVGLLRTLYPKWSPAAIRSAIMTTAETKANDLNPILSSSQV 557
           R P+ AESGTSMSCPHVSGIVGLL+T YPKWSPAAI+SA+MTTA TKANDL+PIL+++Q+
Sbjct: 549 RSPFNAESGTSMSCPHVSGIVGLLKTRYPKWSPAAIKSALMTTAATKANDLHPILNTTQL 608

Query: 558 KATPFAYGAGHVNPNRAANPGLVYDLTTKDYLNFLCAHGYNETQLKQFSNATFACSKTFK 617
           KA P +YGAGHV PN+A NPGLVYDLT KDYLNFLCA GYN+TQ+K+FS+A FACS +FK
Sbjct: 609 KANPLSYGAGHVRPNKALNPGLVYDLTLKDYLNFLCARGYNQTQIKKFSSAPFACSHSFK 668

Query: 618 VTDLNYPSISIPDLKVGTAKVKRRVKNVGSPGTYVAQVKAPPGVSVSVEPSSLKFTGVGE 677
           +TD NYPSISIP+LK G  K KRRVKNVGSPGTYVAQVKAPPGV+VSVEP+ LKFTG+GE
Sbjct: 669 LTDFNYPSISIPNLKFGPVKAKRRVKNVGSPGTYVAQVKAPPGVAVSVEPNMLKFTGIGE 728

Query: 678 EKSFRVVLKRVSNDYHRGYVFGRLAWTDGKHRVRSPIVVNLG 720
           E+SFRVV++RV N+  RGYVFG LAW+DG HRVRSPI VNLG
Sbjct: 729 EQSFRVVVRRVENEERRGYVFGWLAWSDGNHRVRSPIAVNLG 768

BLAST of Lcy11g016400 vs. ExPASy TrEMBL
Match: A0A5D3D7B7 (Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00140 PE=3 SV=1)

HSP 1 Score: 1113.2 bits (2878), Expect = 0.0e+00
Identity = 543/704 (77.13%), Postives = 612/704 (86.93%), Query Frame = 0

Query: 16  QTAKEAIFYSYNRHINGFAAMLDQKAAEDLAKHPAVASINENKGRKLHTTSSWNFLGVEH 75
           + AKEAIFYSYNRHINGFAA+LDQK  EDLA++PAVASI+ENKGRKLHTTSSW FLGVEH
Sbjct: 42  EAAKEAIFYSYNRHINGFAAILDQKVVEDLARNPAVASIHENKGRKLHTTSSWKFLGVEH 101

Query: 76  DDGIPANSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPIPTRWRGSCEGGSKFHCN 135
           DDGIP NSIWN ASFGESTIIGNLDTGVWPESKSF+DEGYGP+PTRW+GSCEGGSKFHCN
Sbjct: 102 DDGIPHNSIWNRASFGESTIIGNLDTGVWPESKSFNDEGYGPVPTRWKGSCEGGSKFHCN 161

Query: 136 SRKLIGARYFNKGYAAYVGSLNASFETARDHDGHGTHTLSTAGGNFISGASVFGNGNGTA 195
            RKLIGARYFNKGYAAY GSLNAS+ETARD++GHGTHTLSTAGGNFISGA+VFGNGNGTA
Sbjct: 162 -RKLIGARYFNKGYAAYAGSLNASYETARDNEGHGTHTLSTAGGNFISGANVFGNGNGTA 221

Query: 196 KGGSPKARVVAYKVCWPKIDSSGGCFDADILAAIEAAITDGVDVLSISLGGDSKDFFDDV 255
           KGGSPKA V AYKVCWP++DS GGCFDADILAAIEAAI+DGVD+LS+SLGG +KDF +DV
Sbjct: 222 KGGSPKALVAAYKVCWPQVDSGGGCFDADILAAIEAAISDGVDILSLSLGGGAKDFSEDV 281

Query: 256 NAIGAFHAVQHGIIVVCSAGNSGPAPGTVENVAPWLVTVGASTINRQFTSYVALGNKTHI 315
            AIGAFHAVQ GIIVVCSAGNSGPAPGT+EN APW++TVGASTINR FTSYVALGNK HI
Sbjct: 282 TAIGAFHAVQQGIIVVCSAGNSGPAPGTIENGAPWILTVGASTINRDFTSYVALGNKKHI 341

Query: 316 KGASLSDKILPSQKFYPLINSVDAKASSVSNNNAQLCEEGSLDPKKVKGKILVCLRGENG 375
           K   L   IL    +     S++    ++ N  +QLC+ GSLDPKKVKGKI++CLRGEN 
Sbjct: 342 KVLFLIKFILMGSSY-----SIE---KTLMNPCSQLCQAGSLDPKKVKGKIILCLRGENA 401

Query: 376 RVDKGFVAAQAGAVGMILANSEQNGDELTADAHLLPASHVSYTDGRSVYQYINSTKIPMA 435
           RVDKG+ AAQAGAVGMILAN+EQNGDEL ADAHLLP SHVSYTDG+S+YQYIN TK PMA
Sbjct: 402 RVDKGYAAAQAGAVGMILANAEQNGDELIADAHLLPVSHVSYTDGQSIYQYINFTKTPMA 461

Query: 436 FMTRVRTEVGVKPAPFMAEFSSRGPNEIEASILKPDITAPGVSIIAAFAGDLSPTGEDLD 495
           +MT VRTE+G+KPAP MA FSSRGPN IE SILKPDITAPGV+I+AA++ D SP+G   D
Sbjct: 462 YMTHVRTELGIKPAPVMASFSSRGPNTIEESILKPDITAPGVNILAAYSEDASPSGSLFD 521

Query: 496 TRRIPYMAESGTSMSCPHVSGIVGLLRTLYPKWSPAAIRSAIMTTAETKANDLNPILSSS 555
            RRIP+   SGTSMSCPH+SGIVGLL+TLYP WSPAAI+SAIMTTAET+ANDL+PIL++ 
Sbjct: 522 NRRIPFNIVSGTSMSCPHISGIVGLLKTLYPNWSPAAIKSAIMTTAETRANDLHPILNTV 581

Query: 556 QVKATPFAYGAGHVNPNRAANPGLVYDLTTKDYLNFLCAHGYNETQLKQFSNATFACSKT 615
            +KA PFAYGAGHV PNRA NPGLVYDLTT DY+NFLCA GYN++Q+ +FS  +F CSK+
Sbjct: 582 NLKANPFAYGAGHVQPNRAMNPGLVYDLTTNDYMNFLCAQGYNKSQISKFSATSFVCSKS 641

Query: 616 FKVTDLNYPSISIPDLKVGTAKVKRRVKNVGSPGTYVAQVKAPPGVSVSVEPSSLKFTGV 675
           FK+TD NYPSISIPD+K G   + RRVKNVG P TYVA+VK P GVSVSVEP +LKFTG+
Sbjct: 642 FKLTDFNYPSISIPDMKSGVVTINRRVKNVGKPSTYVARVKVPQGVSVSVEPRTLKFTGI 701

Query: 676 GEEKSFRVVLKRVSNDYHRGYVFGRLAWTDGKHRVRSPIVVNLG 720
            EEKSF+VV+  V+N+ HRGYVFG L W DGKH VRSPIVVNLG
Sbjct: 702 DEEKSFKVVIGSVANNKHRGYVFGSLIWEDGKHHVRSPIVVNLG 736

BLAST of Lcy11g016400 vs. ExPASy TrEMBL
Match: A0A0A0L2R9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045120 PE=3 SV=1)

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 531/707 (75.11%), Postives = 616/707 (87.13%), Query Frame = 0

Query: 16  QTAKEAIFYSYNRHINGFAAMLDQKAAEDLAKHPAVASINENKGRKLHTTSSWNFLGVEH 75
           + AKEAIFYSYNRHINGFAA++DQK AEDLAKHP V S+ ENKGRKLHTT+SW FLG+E+
Sbjct: 54  EEAKEAIFYSYNRHINGFAAVVDQKVAEDLAKHPDVVSVLENKGRKLHTTNSWKFLGLEN 113

Query: 76  DDGIPANSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPIPTRWRGSCEGGSKFHCN 135
           +  IP+NS+WNLASFGESTIIGNLDTGVWPESKSFSD+ YGPIP+RW+GSCEGGSKF+CN
Sbjct: 114 NGAIPSNSLWNLASFGESTIIGNLDTGVWPESKSFSDKEYGPIPSRWKGSCEGGSKFYCN 173

Query: 136 SRKLIGARYFNKGYAAYVGSLNASFETARDHDGHGTHTLSTAGGNFISGASVFGNGNGTA 195
            RKLIGARY+NKGYAA VG LN+S+E+ARDH+GHGTHTLSTAGG+F+  A++FG GNGTA
Sbjct: 174 -RKLIGARYYNKGYAAIVGPLNSSYESARDHEGHGTHTLSTAGGHFVPNANLFGYGNGTA 233

Query: 196 KGGSPKARVVAYKVCWPKIDSSGGCFDADILAAIEAAITDGVDVLSISLGGDSKDFFDDV 255
           KGGSPKA V AYKVCWP++   G CFDADILA  EAAI DGVDVLS+SLGG   DF  D 
Sbjct: 234 KGGSPKALVAAYKVCWPQVLFFGECFDADILAGFEAAIGDGVDVLSVSLGGSPSDFAKDS 293

Query: 256 NAIGAFHAVQHGIIVVCSAGNSGPAPGTVENVAPWLVTVGASTINRQFTSYVALGNKTHI 315
            +IG+FHAVQ+GI+VVCSAGNSGP PG+V NVAPW++TVGAST +R +TSYVA+G+K H 
Sbjct: 294 ISIGSFHAVQNGIVVVCSAGNSGPTPGSVSNVAPWIITVGASTTDRLYTSYVAIGDKRHF 353

Query: 316 KGASLSDKILPSQKFYPLINSVDAKASSVSNNNAQLCEEGSLDPKKVKGKILVCLRGENG 375
           KGAS+SDK LP QKFYPLI+S+DAKA +V++N+A LCEEGSLDPKKV GKI++CLRG+N 
Sbjct: 354 KGASVSDKKLPVQKFYPLISSLDAKAKNVTDNDALLCEEGSLDPKKVNGKIIICLRGDNA 413

Query: 376 RVDKGFVAAQAGAVGMILANSEQNGDELTADAHLLPASHVSYTDGRSVYQYINSTKIPMA 435
           RV KG+VAA+AGAVGMILAN+E+NGDE+ ADAHLLPASH++Y+DG+ VYQYINSTKIPMA
Sbjct: 414 RVAKGYVAAKAGAVGMILANAEENGDEILADAHLLPASHITYSDGQLVYQYINSTKIPMA 473

Query: 436 FMTRVRTEVGVKPAPFMAEFSSRGPNEIEASILKPDITAPGVSIIAAFAGDLSPTGEDLD 495
           +MT VRTE G+KPAP MA FSSRGPN ++ SILKPDITAPG +I+AA++ D SPTG D D
Sbjct: 474 YMTHVRTEEGIKPAPVMASFSSRGPNTVDPSILKPDITAPGENILAAYSRDASPTGTDFD 533

Query: 496 TRRIPYMAESGTSMSCPHVSGIVGLLRTLYPKWSPAAIRSAIMTTAETKANDLNPILSSS 555
            RR+P+  ESGTSMSCPHVSGIVGLL+TLYPKWSPAAIRSAIMTTA TKANDL PILS++
Sbjct: 534 KRRVPFNVESGTSMSCPHVSGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLTPILSTN 593

Query: 556 QVKATPFAYGAGHVNPNRAANPGLVYDLTTKDYLNFLCAHGYNETQLKQFSNAT-FACSK 615
           Q KA  FAYGAGHV+PNRAA+PGLVYDL+TKDYLN+LCA GYN  Q+KQFSN T F CSK
Sbjct: 594 QEKANAFAYGAGHVSPNRAADPGLVYDLSTKDYLNYLCAQGYNTAQIKQFSNDTSFVCSK 653

Query: 616 TFKVTDLNYPSISIPDLKVGTA-KVKRRVKNVGSPGTYVAQVKAPPGVSVSVEPSSLKFT 675
           +FK+TDLNYPSISIP L+   A K+KR++KNVGSPGTYV QVK P GVSVSVEP+SLKFT
Sbjct: 654 SFKLTDLNYPSISIPILEYDVAVKIKRKLKNVGSPGTYVVQVKEPLGVSVSVEPTSLKFT 713

Query: 676 GVGEEKSFRVVLKRVS-NDYHRGYVFGRLAWTDGKHRVRSPIVVNLG 720
           G+ EEKSFRVVLK    N     Y+FG+L W+DGKHRVRSPIVV LG
Sbjct: 714 GIDEEKSFRVVLKSYEPNGSGPKYLFGKLEWSDGKHRVRSPIVVRLG 759

BLAST of Lcy11g016400 vs. ExPASy TrEMBL
Match: A0A6J1EWX5 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111438769 PE=3 SV=1)

HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 530/705 (75.18%), Postives = 602/705 (85.39%), Query Frame = 0

Query: 16  QTAKEAIFYSYNRHINGFAAMLDQKAAEDLAKHPAVASINENKGRKLHTTSSWNFLGVEH 75
           + AKEAIFYSY+RHINGFAA+LD K A+DL +HPAV S++ENK RKLHTTSSW FL +E+
Sbjct: 67  EIAKEAIFYSYHRHINGFAAVLDHKVAQDLTRHPAVVSVHENKMRKLHTTSSWEFLDLEN 126

Query: 76  DDGIPANSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPIPTRWRGSCEGGSKFHCN 135
            +G P NSIWN A+FGESTII NLDTGVWPESKSF+DEGYG IP+RWRGSCEGGS FHCN
Sbjct: 127 GEGTPPNSIWNGANFGESTIIANLDTGVWPESKSFNDEGYGAIPSRWRGSCEGGSNFHCN 186

Query: 136 SRKLIGARYFNKGYAAYVGSLNASFETARDHDGHGTHTLSTAGGNFISGASVFGNGNGTA 195
            RKLIGARYFNKGY A  GSL+ SF+TARDHDGHGTHTLSTAGGNF+SGA+VFGNGNGTA
Sbjct: 187 -RKLIGARYFNKGYTALAGSLDDSFDTARDHDGHGTHTLSTAGGNFVSGANVFGNGNGTA 246

Query: 196 KGGSPKARVVAYKVCWPKIDSSGGCFDADILAAIEAAITDGVDVLSISLGGDSKDFFDDV 255
           KGGSPKA V AYKVCWP     G C DADILAAIEAAITDGVDVLS+SLG  S +FFDDV
Sbjct: 247 KGGSPKAFVAAYKVCWPTF-HGGQCSDADILAAIEAAITDGVDVLSLSLGRGSTEFFDDV 306

Query: 256 NAIGAFHAVQHGIIVVCSAGNSGPAPGTVENVAPWLVTVGASTINRQFTSYVALGNKTHI 315
            AIG+FHAVQ GI+VVCS GNSGP P +VENVAPWL TV ASTI RQFTSYVALGNK HI
Sbjct: 307 TAIGSFHAVQQGIVVVCSGGNSGPDPQSVENVAPWLFTVAASTITRQFTSYVALGNKKHI 366

Query: 316 KGASLSDKILPSQKFYPLINSVDAKASSVSNNNAQLCEEGSLDPKKVKGKILVCLR-GEN 375
            GAS+SDKILP+Q+FYPLI SVDAKA ++S   A+LC EGSLDP+KVKGKI+VC++ G++
Sbjct: 367 TGASISDKILPAQQFYPLITSVDAKAINISVETAKLCVEGSLDPRKVKGKIIVCVKGGDS 426

Query: 376 GRVDKGFVAAQAGAVGMILANSEQNGDELTADAHLLPASHVSYTDGRSVYQYINSTKIPM 435
            RVDKG+VAAQAGAVGMILANSE++G+EL ADAHLLP SH+SY DG  VY+YINSTK P+
Sbjct: 427 ARVDKGYVAAQAGAVGMILANSEEDGNELIADAHLLPVSHISYIDGEIVYEYINSTKTPV 486

Query: 436 AFMTRVRTEVGVKPAPFMAEFSSRGPNEIEASILKPDITAPGVSIIAAFAGDLSPTGEDL 495
           A+MT+VRTE G+KPAP MA FSSRGPN IE SILKPDITAPGV+IIAA++ D SP+G   
Sbjct: 487 AYMTQVRTETGIKPAPVMASFSSRGPNSIEESILKPDITAPGVNIIAAYSEDASPSGSPF 546

Query: 496 DTRRIPYMAESGTSMSCPHVSGIVGLLRTLYPKWSPAAIRSAIMTTAETKANDLNPILSS 555
           D RRIP+   SGTSMSCPH+SGIV LL+T+YPKWSPAAI+SAIMTTAET+ANDL+PIL S
Sbjct: 547 DNRRIPFNVVSGTSMSCPHISGIVALLKTIYPKWSPAAIKSAIMTTAETRANDLHPILDS 606

Query: 556 SQVKATPFAYGAGHVNPNRAANPGLVYDLTTKDYLNFLCAHGYNETQLKQFSNATFACSK 615
           +++ A P AYGAGHV+PNRAANPGLVYDLTT DYLNFLCA GYN+TQL +FSN +F CSK
Sbjct: 607 TKLVANPLAYGAGHVHPNRAANPGLVYDLTTNDYLNFLCARGYNKTQLSKFSNTSFVCSK 666

Query: 616 TFKVTDLNYPSISIPDLKVGTAKVKRRVKNVGSPGTYVAQVKAPPGVSVSVEPSSLKFTG 675
           +FK+TD NYPSISIP +K     +KR VKNVGSP TYVA+VK PPGV VSVEPS+LKFT 
Sbjct: 667 SFKLTDFNYPSISIPKMKSEPVTIKRTVKNVGSPSTYVARVKVPPGVLVSVEPSTLKFTR 726

Query: 676 VGEEKSFRVVLKRVSNDYHRGYVFGRLAWTDGKHRVRSPIVVNLG 720
             EEK+F+VV + V+N  HRGYVFG L W DGKH VRS IVVNLG
Sbjct: 727 TDEEKTFKVVFRSVTNTKHRGYVFGSLKWLDGKHHVRSSIVVNLG 769

BLAST of Lcy11g016400 vs. NCBI nr
Match: XP_038904203.1 (uncharacterized protein LOC120090550 [Benincasa hispida])

HSP 1 Score: 1174.8 bits (3038), Expect = 0.0e+00
Identity = 568/705 (80.57%), Postives = 635/705 (90.07%), Query Frame = 0

Query: 16   QTAKEAIFYSYNRHINGFAAMLDQKAAEDLAKHPAVASINENKGRKLHTTSSWNFLGVEH 75
            + AKEAIFYSYNRHINGFAAMLD K AEDLA++PAVAS++ENKGRKLHTTSSW FLGVEH
Sbjct: 881  EAAKEAIFYSYNRHINGFAAMLDHKVAEDLARNPAVASVHENKGRKLHTTSSWKFLGVEH 940

Query: 76   DDGIPANSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPIPTRWRGSCEGGSKFHCN 135
            DDGIP  SIWNLASFGESTII NLDTGVWPESKSFSDEGYGP+PTRW+GSCEGGSKFHCN
Sbjct: 941  DDGIPTKSIWNLASFGESTIIANLDTGVWPESKSFSDEGYGPVPTRWKGSCEGGSKFHCN 1000

Query: 136  SRKLIGARYFNKGYAAYVGSLNASFETARDHDGHGTHTLSTAGGNFISGASVFGNGNGTA 195
             RKLIGARYFNKGYAAYVGSLNA++ETARDHDGHGTHTLSTAGGNFISGA+VFGNGNGTA
Sbjct: 1001 -RKLIGARYFNKGYAAYVGSLNATYETARDHDGHGTHTLSTAGGNFISGANVFGNGNGTA 1060

Query: 196  KGGSPKARVVAYKVCWPKIDSSGGCFDADILAAIEAAITDGVDVLSISLGGDSKDFFDDV 255
            KGGSPKA V AYKVCWP +DS G CFDADILAAIEAAI+DGVDVLS+SLGGDSKDF DDV
Sbjct: 1061 KGGSPKALVAAYKVCWPPVDSGGSCFDADILAAIEAAISDGVDVLSLSLGGDSKDFSDDV 1120

Query: 256  NAIGAFHAVQHGIIVVCSAGNSGPAPGTVENVAPWLVTVGASTINRQFTSYVALGNKTHI 315
             AIGAFHAVQ GI+VVCSAGNSGPAPGTVENVAPW+ TVGASTINR+FTSYVALGNK HI
Sbjct: 1121 AAIGAFHAVQQGIVVVCSAGNSGPAPGTVENVAPWIFTVGASTINREFTSYVALGNKKHI 1180

Query: 316  KGASLSDKILPSQKFYPLINSVDAKASSVSNNNAQLCEEGSLDPKKVKGKILVCLRGENG 375
            KGASLSDKILP+QKFYPLI++  AKA+ VS ++AQLCEEGSLDP+KVKGKI++CLRGEN 
Sbjct: 1181 KGASLSDKILPAQKFYPLISAAYAKANHVSFDDAQLCEEGSLDPRKVKGKIIICLRGENA 1240

Query: 376  RVDKGFVAAQAGAVGMILANSEQNGDELTADAHLLPASHVSYTDGRSVYQYINSTKIPMA 435
            RVDKG+VAAQAGAVGMILAN+EQN DEL ADAHLLP SHVSYTDG+S+YQYINSTK PMA
Sbjct: 1241 RVDKGYVAAQAGAVGMILANTEQNEDELIADAHLLPVSHVSYTDGQSIYQYINSTKTPMA 1300

Query: 436  FMTRVRTEVGVKPAPFMAEFSSRGPNEIEASILKPDITAPGVSIIAAFAGDLSPTGEDLD 495
            +MT  RTE+G+KPAP MA FSSRGPN +E SILKPDITAPGV+I+AA++ D SP+G   D
Sbjct: 1301 YMTHARTELGIKPAPVMASFSSRGPNTMEESILKPDITAPGVNILAAYSEDASPSGSSFD 1360

Query: 496  TRRIPYMAESGTSMSCPHVSGIVGLLRTLYPKWSPAAIRSAIMTTAETKANDLNPILSSS 555
             RRIP+   SGTSMSCPH+SGIVGLL+TLYPKWSPAAI+SAIMTTAET+AND +PIL++ 
Sbjct: 1361 NRRIPFNIVSGTSMSCPHISGIVGLLKTLYPKWSPAAIKSAIMTTAETRANDKHPILNTE 1420

Query: 556  QVKATPFAYGAGHVNPNRAANPGLVYDLTTKDYLNFLCAHGYNETQLKQFSNATFACSKT 615
            ++KA PFAYGAGHV PNRA NPGLVYDLTTKDYLNFLC  GYN+TQ+ +FSN +F CSK+
Sbjct: 1421 ELKANPFAYGAGHVQPNRAMNPGLVYDLTTKDYLNFLCVLGYNKTQISKFSNTSFVCSKS 1480

Query: 616  FKVTDLNYPSISIPDLKVGTAKVKRRVKNVGSPGTYVAQVKAPPGVSVSVEPSSLKFTGV 675
            FK+TD NYPSISIP++K G   +KRRVKNVG P TYVA+VK PPG SVSV+PS+LKFTG+
Sbjct: 1481 FKLTDFNYPSISIPNMKPGLVTIKRRVKNVGKPSTYVARVKVPPGASVSVKPSTLKFTGI 1540

Query: 676  GEEKSFRVVLKRVSNDYH-RGYVFGRLAWTDGKHRVRSPIVVNLG 720
             EEKSF+V++  V+++ H RGYVFG L W DGKH VRSPIVVNLG
Sbjct: 1541 DEEKSFKVIVGSVADNKHSRGYVFGSLVWEDGKHHVRSPIVVNLG 1584

BLAST of Lcy11g016400 vs. NCBI nr
Match: XP_016900594.1 (PREDICTED: subtilisin-like protease SBT5.3, partial [Cucumis melo])

HSP 1 Score: 1167.5 bits (3019), Expect = 0.0e+00
Identity = 563/704 (79.97%), Postives = 631/704 (89.63%), Query Frame = 0

Query: 16  QTAKEAIFYSYNRHINGFAAMLDQKAAEDLAKHPAVASINENKGRKLHTTSSWNFLGVEH 75
           + AKEAIFYSYNRHINGFAA+LDQK  EDLA++PAVASI+ENKGRKLHTTSSW FLGVEH
Sbjct: 45  EAAKEAIFYSYNRHINGFAAILDQKVVEDLARNPAVASIHENKGRKLHTTSSWKFLGVEH 104

Query: 76  DDGIPANSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPIPTRWRGSCEGGSKFHCN 135
           DDGIP NSIWN ASFGESTIIGNLDTGVWPESKSF+DEGYGP+PTRW+GSCEGGSKFHCN
Sbjct: 105 DDGIPHNSIWNRASFGESTIIGNLDTGVWPESKSFNDEGYGPVPTRWKGSCEGGSKFHCN 164

Query: 136 SRKLIGARYFNKGYAAYVGSLNASFETARDHDGHGTHTLSTAGGNFISGASVFGNGNGTA 195
            RKLIGARYFNKGYAAY GSLNAS+ETARD++GHGTHTLSTAGGNFISGA+VFGNGNGTA
Sbjct: 165 -RKLIGARYFNKGYAAYAGSLNASYETARDNEGHGTHTLSTAGGNFISGANVFGNGNGTA 224

Query: 196 KGGSPKARVVAYKVCWPKIDSSGGCFDADILAAIEAAITDGVDVLSISLGGDSKDFFDDV 255
           KGGSPKA V AYKVCWP++DS GGCFDADILAAIEAAI+DGVD+LS+SLGG +KDF +DV
Sbjct: 225 KGGSPKALVAAYKVCWPQVDSGGGCFDADILAAIEAAISDGVDILSLSLGGGAKDFSEDV 284

Query: 256 NAIGAFHAVQHGIIVVCSAGNSGPAPGTVENVAPWLVTVGASTINRQFTSYVALGNKTHI 315
            AIGAFHAVQ GIIVVCSAGNSGPAPGT+EN APW++TVGASTINR FTSYVALGNK HI
Sbjct: 285 TAIGAFHAVQQGIIVVCSAGNSGPAPGTIENGAPWILTVGASTINRDFTSYVALGNKKHI 344

Query: 316 KGASLSDKILPSQKFYPLINSVDAKASSVSNNNAQLCEEGSLDPKKVKGKILVCLRGENG 375
           KGASLSDKILP QKFYPLIN+ DAKA++VS++ AQLC+ GSLDPKKVKGKI++CLRGEN 
Sbjct: 345 KGASLSDKILPEQKFYPLINAADAKANNVSSDVAQLCQAGSLDPKKVKGKIILCLRGENA 404

Query: 376 RVDKGFVAAQAGAVGMILANSEQNGDELTADAHLLPASHVSYTDGRSVYQYINSTKIPMA 435
           RVDKG+ AAQAGAVGMILAN+EQNGDEL ADAHLLP SHVSYTDG+S+YQYIN TK PMA
Sbjct: 405 RVDKGYAAAQAGAVGMILANAEQNGDELIADAHLLPVSHVSYTDGQSIYQYINFTKTPMA 464

Query: 436 FMTRVRTEVGVKPAPFMAEFSSRGPNEIEASILKPDITAPGVSIIAAFAGDLSPTGEDLD 495
           +MT VRTE+G+KPAP MA FSSRGPN IE SILKPDITAPGV+I+AA++ D SP+G   D
Sbjct: 465 YMTHVRTELGIKPAPVMASFSSRGPNTIEESILKPDITAPGVNILAAYSEDASPSGSLFD 524

Query: 496 TRRIPYMAESGTSMSCPHVSGIVGLLRTLYPKWSPAAIRSAIMTTAETKANDLNPILSSS 555
            RRIP+   SGTSMSCPH+SGIVGLL+TLYP WSPAAI+SAIMTTAET+ANDL+PIL++ 
Sbjct: 525 NRRIPFNIVSGTSMSCPHISGIVGLLKTLYPTWSPAAIKSAIMTTAETRANDLHPILNTV 584

Query: 556 QVKATPFAYGAGHVNPNRAANPGLVYDLTTKDYLNFLCAHGYNETQLKQFSNATFACSKT 615
            +KA PFAYGAGHV PNRA NPGLVYDLTT DY+NFLCA GYN++Q+ +FS  +F CSK+
Sbjct: 585 NLKANPFAYGAGHVQPNRAMNPGLVYDLTTNDYMNFLCAQGYNKSQISKFSATSFVCSKS 644

Query: 616 FKVTDLNYPSISIPDLKVGTAKVKRRVKNVGSPGTYVAQVKAPPGVSVSVEPSSLKFTGV 675
           FK+TD NYPSISIPD+K G   + RRVKNVG P TYVA+VK P GVSVSVEP +LKFTG+
Sbjct: 645 FKLTDFNYPSISIPDMKSGVVTINRRVKNVGKPSTYVARVKVPQGVSVSVEPRTLKFTGI 704

Query: 676 GEEKSFRVVLKRVSNDYHRGYVFGRLAWTDGKHRVRSPIVVNLG 720
            EEKSF+VV+  V+N+ HRGYVFG L W DGKH VRSPIVVNLG
Sbjct: 705 DEEKSFKVVIGSVANNKHRGYVFGSLIWEDGKHHVRSPIVVNLG 747

BLAST of Lcy11g016400 vs. NCBI nr
Match: XP_022143461.1 (subtilisin-like protease SBT5.4 [Momordica charantia])

HSP 1 Score: 1123.6 bits (2905), Expect = 0.0e+00
Identity = 546/702 (77.78%), Postives = 622/702 (88.60%), Query Frame = 0

Query: 18  AKEAIFYSYNRHINGFAAMLDQKAAEDLAKHPAVASINENKGRKLHTTSSWNFLGVEHDD 77
           AK+AIFYSYN++INGFAA+LD+K A++LAKHP+V S++ENK RKLHTT SW+FLG+E+D 
Sbjct: 69  AKDAIFYSYNKYINGFAAILDEKVAQELAKHPSVVSVHENKARKLHTTGSWSFLGLENDG 128

Query: 78  GIPANSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPIPTRWRGSCEGGSKFHCNSR 137
            IP+NSIWNLASFGESTIIGNLDTGVWPESKSFSD+GYGPIP+RWRGSCEGGSKF CN R
Sbjct: 129 EIPSNSIWNLASFGESTIIGNLDTGVWPESKSFSDKGYGPIPSRWRGSCEGGSKFRCN-R 188

Query: 138 KLIGARYFNKGYAAYVGSLNASFETARDHDGHGTHTLSTAGGNFISGASVFGNGNGTAKG 197
           KLIGARYFNKGY A +G LNAS+ETARD +GHGTHTLSTAGGNF+SGASVFGNGNGTAKG
Sbjct: 189 KLIGARYFNKGYVASIGPLNASYETARDDEGHGTHTLSTAGGNFVSGASVFGNGNGTAKG 248

Query: 198 GSPKARVVAYKVCWPKIDSSGGCFDADILAAIEAAITDGVDVLSISLGGDSKDFFDDVNA 257
           GSP+ARV AY+VCWP +  +GGCF ADILA  EAAI DGVDVLS+SLGG  ++F DD  A
Sbjct: 249 GSPRARVAAYRVCWPAV-LTGGCFMADILAGFEAAIHDGVDVLSVSLGGSPEEFSDDGLA 308

Query: 258 IGAFHAVQHGIIVVCSAGNSGPAPGTVENVAPWLVTVGASTINRQFTSYVALGNKTHIKG 317
           IGAFHAVQHGI VVCSAGNSGP  GTV NVAPW++TVGAST +R F SYV LGN+ HIKG
Sbjct: 309 IGAFHAVQHGITVVCSAGNSGPGRGTVSNVAPWMITVGASTADRLFASYVGLGNRKHIKG 368

Query: 318 ASLSDKILPSQKFYPLINSVDAKASSVSNNNAQLCEEGSLDPKKVKGKILVCLRGENGRV 377
           ASLSDKILP+QKFYPLI++ DAKAS+VS   AQLCEEGSLDPKKV+GKI+VCLRG+N RV
Sbjct: 369 ASLSDKILPAQKFYPLISTADAKASNVSLEYAQLCEEGSLDPKKVEGKIVVCLRGDNARV 428

Query: 378 DKGFVAAQAGAVGMILANSEQNGDELTADAHLLPASHVSYTDGRSVYQYINSTKIPMAFM 437
           DKG+VAAQAGAVGMILAN++ NGDEL ADAHLLPASHVSY DG  ++QYI STKIPMA+M
Sbjct: 429 DKGYVAAQAGAVGMILANAQDNGDELLADAHLLPASHVSYADGELIFQYIKSTKIPMAYM 488

Query: 438 TRVRTEVGVKPAPFMAEFSSRGPNEIEASILKPDITAPGVSIIAAFAGDLSPTGEDLDTR 497
           T V+TE+GVKPAPFMA FSSRGPN IE SILKPDI APGVSIIAA++ + SP+G   D R
Sbjct: 489 THVKTELGVKPAPFMASFSSRGPNTIEESILKPDIIAPGVSIIAAYSEEASPSGSSFDKR 548

Query: 498 RIPYMAESGTSMSCPHVSGIVGLLRTLYPKWSPAAIRSAIMTTAETKANDLNPILSSSQV 557
           R P+ AESGTSMSCPHVSGIVGLL+T YPKWSPAAI+SA+MTTA TKANDL+PIL+++Q+
Sbjct: 549 RSPFNAESGTSMSCPHVSGIVGLLKTRYPKWSPAAIKSALMTTAATKANDLHPILNTTQL 608

Query: 558 KATPFAYGAGHVNPNRAANPGLVYDLTTKDYLNFLCAHGYNETQLKQFSNATFACSKTFK 617
           KA P +YGAGHV PN+A NPGLVYDLT KDYLNFLCA GYN+TQ+K+FS+A FACS +FK
Sbjct: 609 KANPLSYGAGHVRPNKALNPGLVYDLTLKDYLNFLCARGYNQTQIKKFSSAPFACSHSFK 668

Query: 618 VTDLNYPSISIPDLKVGTAKVKRRVKNVGSPGTYVAQVKAPPGVSVSVEPSSLKFTGVGE 677
           +TD NYPSISIP+LK G  K KRRVKNVGSPGTYVAQVKAPPGV+VSVEP+ LKFTG+GE
Sbjct: 669 LTDFNYPSISIPNLKFGPVKAKRRVKNVGSPGTYVAQVKAPPGVAVSVEPNMLKFTGIGE 728

Query: 678 EKSFRVVLKRVSNDYHRGYVFGRLAWTDGKHRVRSPIVVNLG 720
           E+SFRVV++RV N+  RGYVFG LAW+DG HRVRSPI VNLG
Sbjct: 729 EQSFRVVVRRVENEERRGYVFGWLAWSDGNHRVRSPIAVNLG 768

BLAST of Lcy11g016400 vs. NCBI nr
Match: XP_038904154.1 (subtilisin-like protease SBT5.4 [Benincasa hispida])

HSP 1 Score: 1120.5 bits (2897), Expect = 0.0e+00
Identity = 549/707 (77.65%), Postives = 623/707 (88.12%), Query Frame = 0

Query: 16  QTAKEAIFYSYNRHINGFAAMLDQKAAEDLAKHPAVASINENKGRKLHTTSSWNFLGVEH 75
           + AKEAIFYSYNRHINGFAA+LDQK A DL KHP V S++ENKG+KLHTT SWNFLGVE+
Sbjct: 67  EAAKEAIFYSYNRHINGFAAVLDQKVASDLTKHPDVVSVHENKGKKLHTTLSWNFLGVEN 126

Query: 76  DDGIPANSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPIPTRWRGSCEGGSKFHCN 135
           +  IP+NSIWNLASFGESTII NLDTGVWPESKSF+DEGYGPIPTRW+GSCEGGSKFHCN
Sbjct: 127 NGAIPSNSIWNLASFGESTIIANLDTGVWPESKSFNDEGYGPIPTRWKGSCEGGSKFHCN 186

Query: 136 SRKLIGARYFNKGYAAYVGSLNASFETARDHDGHGTHTLSTAGGNFISGASVFGNGNGTA 195
            RKLIGARYFNKGYAA VG LN+S+E+ARDH+GHGTHTLSTAGG+F+ GA++FG GNGTA
Sbjct: 187 -RKLIGARYFNKGYAAIVGPLNSSYESARDHEGHGTHTLSTAGGHFVEGANIFGYGNGTA 246

Query: 196 KGGSPKARVVAYKVCWPKIDSSGGCFDADILAAIEAAITDGVDVLSISLGGDSKDFFDDV 255
           KGGSPKA V AYKVCWP++   G CF+ADILA  EAAI+DGVDVLS+SLGGD  DF  D 
Sbjct: 247 KGGSPKALVAAYKVCWPQVFLFGECFEADILAGFEAAISDGVDVLSVSLGGDPSDFAQDS 306

Query: 256 NAIGAFHAVQHGIIVVCSAGNSGPAPGTVENVAPWLVTVGASTINRQFTSYVALGNKTHI 315
            +IGAFHAVQ+GI VVCSAGNSGP PG+V NVAPW++TVGASTI+R FTSYVALG+K HI
Sbjct: 307 ISIGAFHAVQNGITVVCSAGNSGPIPGSVSNVAPWIITVGASTIDRLFTSYVALGDKKHI 366

Query: 316 KGASLSDKILPSQKFYPLINSVDAKASSVSNNNAQLCEEGSLDPKKVKGKILVCLRGENG 375
           KGASLSDKILP QKFYPLI+S+DAKA++VSN +A +CEEGSLDPKKVKGKI+VCLRG N 
Sbjct: 367 KGASLSDKILPEQKFYPLISSLDAKANNVSNYHALICEEGSLDPKKVKGKIVVCLRGINA 426

Query: 376 RVDKGFVAAQAGAVGMILANSEQNGDELTADAHLLPASHVSYTDGRSVYQYINSTKIPMA 435
           RVDKGFVAA+AGAVGMILAN ++NGDEL ADAHLLPASH++Y+DG+ VYQYINSTKIPMA
Sbjct: 427 RVDKGFVAAEAGAVGMILANDKENGDELLADAHLLPASHITYSDGQLVYQYINSTKIPMA 486

Query: 436 FMTRVRTEVGVKPAPFMAEFSSRGPNEIEASILKPDITAPGVSIIAAFAGDLSPTGEDLD 495
           ++T VRTE+GVKPAP MA FSSRGPN I+ SILKPDITAPGV+I+AA++ D SP+G   D
Sbjct: 487 YLTHVRTELGVKPAPVMASFSSRGPNTIDPSILKPDITAPGVNILAAYSEDASPSGSSFD 546

Query: 496 TRRIPYMAESGTSMSCPHVSGIVGLLRTLYPKWSPAAIRSAIMTTAETKANDLNPILSSS 555
            RR+ +  ESGTSMSCPH+SGIVGLL+TLYPKWSPAAIRSAIMTTA TKANDLNPILS+ 
Sbjct: 547 KRRVAFNVESGTSMSCPHISGIVGLLKTLYPKWSPAAIRSAIMTTAGTKANDLNPILSTK 606

Query: 556 QVKATPFAYGAGHVNPNRAANPGLVYDLTTKDYLNFLCAHGYNETQLKQFSNAT-FACSK 615
           Q KA  FAYGAGHV PN+AANPGLVYDL+TKDYLN+LCA GYN+TQ+KQFSN T F CSK
Sbjct: 607 QEKANAFAYGAGHVRPNKAANPGLVYDLSTKDYLNYLCALGYNKTQIKQFSNDTSFVCSK 666

Query: 616 TFKVTDLNYPSISIPDLK-VGTAKVKRRVKNVGSPGTYVAQVKAPPGVSVSVEPSSLKFT 675
           +FK TDLNYPSISIPDLK     K+KRR+KNVGSPGTYV QV APPGVSV VEP+SLKFT
Sbjct: 667 SFKTTDLNYPSISIPDLKSEAVVKIKRRLKNVGSPGTYVVQVNAPPGVSVFVEPTSLKFT 726

Query: 676 GVGEEKSFRVVLK-RVSNDYHRGYVFGRLAWTDGKHRVRSPIVVNLG 720
           G+ EEKSFRVVLK  V ND+ + YVFGR+ W+DG HRV+SPIVV +G
Sbjct: 727 GIDEEKSFRVVLKSSVPNDFRQMYVFGRIEWSDGNHRVKSPIVVRVG 772

BLAST of Lcy11g016400 vs. NCBI nr
Match: TYK19383.1 (subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa])

HSP 1 Score: 1113.2 bits (2878), Expect = 0.0e+00
Identity = 543/704 (77.13%), Postives = 612/704 (86.93%), Query Frame = 0

Query: 16  QTAKEAIFYSYNRHINGFAAMLDQKAAEDLAKHPAVASINENKGRKLHTTSSWNFLGVEH 75
           + AKEAIFYSYNRHINGFAA+LDQK  EDLA++PAVASI+ENKGRKLHTTSSW FLGVEH
Sbjct: 42  EAAKEAIFYSYNRHINGFAAILDQKVVEDLARNPAVASIHENKGRKLHTTSSWKFLGVEH 101

Query: 76  DDGIPANSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPIPTRWRGSCEGGSKFHCN 135
           DDGIP NSIWN ASFGESTIIGNLDTGVWPESKSF+DEGYGP+PTRW+GSCEGGSKFHCN
Sbjct: 102 DDGIPHNSIWNRASFGESTIIGNLDTGVWPESKSFNDEGYGPVPTRWKGSCEGGSKFHCN 161

Query: 136 SRKLIGARYFNKGYAAYVGSLNASFETARDHDGHGTHTLSTAGGNFISGASVFGNGNGTA 195
            RKLIGARYFNKGYAAY GSLNAS+ETARD++GHGTHTLSTAGGNFISGA+VFGNGNGTA
Sbjct: 162 -RKLIGARYFNKGYAAYAGSLNASYETARDNEGHGTHTLSTAGGNFISGANVFGNGNGTA 221

Query: 196 KGGSPKARVVAYKVCWPKIDSSGGCFDADILAAIEAAITDGVDVLSISLGGDSKDFFDDV 255
           KGGSPKA V AYKVCWP++DS GGCFDADILAAIEAAI+DGVD+LS+SLGG +KDF +DV
Sbjct: 222 KGGSPKALVAAYKVCWPQVDSGGGCFDADILAAIEAAISDGVDILSLSLGGGAKDFSEDV 281

Query: 256 NAIGAFHAVQHGIIVVCSAGNSGPAPGTVENVAPWLVTVGASTINRQFTSYVALGNKTHI 315
            AIGAFHAVQ GIIVVCSAGNSGPAPGT+EN APW++TVGASTINR FTSYVALGNK HI
Sbjct: 282 TAIGAFHAVQQGIIVVCSAGNSGPAPGTIENGAPWILTVGASTINRDFTSYVALGNKKHI 341

Query: 316 KGASLSDKILPSQKFYPLINSVDAKASSVSNNNAQLCEEGSLDPKKVKGKILVCLRGENG 375
           K   L   IL    +     S++    ++ N  +QLC+ GSLDPKKVKGKI++CLRGEN 
Sbjct: 342 KVLFLIKFILMGSSY-----SIE---KTLMNPCSQLCQAGSLDPKKVKGKIILCLRGENA 401

Query: 376 RVDKGFVAAQAGAVGMILANSEQNGDELTADAHLLPASHVSYTDGRSVYQYINSTKIPMA 435
           RVDKG+ AAQAGAVGMILAN+EQNGDEL ADAHLLP SHVSYTDG+S+YQYIN TK PMA
Sbjct: 402 RVDKGYAAAQAGAVGMILANAEQNGDELIADAHLLPVSHVSYTDGQSIYQYINFTKTPMA 461

Query: 436 FMTRVRTEVGVKPAPFMAEFSSRGPNEIEASILKPDITAPGVSIIAAFAGDLSPTGEDLD 495
           +MT VRTE+G+KPAP MA FSSRGPN IE SILKPDITAPGV+I+AA++ D SP+G   D
Sbjct: 462 YMTHVRTELGIKPAPVMASFSSRGPNTIEESILKPDITAPGVNILAAYSEDASPSGSLFD 521

Query: 496 TRRIPYMAESGTSMSCPHVSGIVGLLRTLYPKWSPAAIRSAIMTTAETKANDLNPILSSS 555
            RRIP+   SGTSMSCPH+SGIVGLL+TLYP WSPAAI+SAIMTTAET+ANDL+PIL++ 
Sbjct: 522 NRRIPFNIVSGTSMSCPHISGIVGLLKTLYPNWSPAAIKSAIMTTAETRANDLHPILNTV 581

Query: 556 QVKATPFAYGAGHVNPNRAANPGLVYDLTTKDYLNFLCAHGYNETQLKQFSNATFACSKT 615
            +KA PFAYGAGHV PNRA NPGLVYDLTT DY+NFLCA GYN++Q+ +FS  +F CSK+
Sbjct: 582 NLKANPFAYGAGHVQPNRAMNPGLVYDLTTNDYMNFLCAQGYNKSQISKFSATSFVCSKS 641

Query: 616 FKVTDLNYPSISIPDLKVGTAKVKRRVKNVGSPGTYVAQVKAPPGVSVSVEPSSLKFTGV 675
           FK+TD NYPSISIPD+K G   + RRVKNVG P TYVA+VK P GVSVSVEP +LKFTG+
Sbjct: 642 FKLTDFNYPSISIPDMKSGVVTINRRVKNVGKPSTYVARVKVPQGVSVSVEPRTLKFTGI 701

Query: 676 GEEKSFRVVLKRVSNDYHRGYVFGRLAWTDGKHRVRSPIVVNLG 720
            EEKSF+VV+  V+N+ HRGYVFG L W DGKH VRSPIVVNLG
Sbjct: 702 DEEKSFKVVIGSVANNKHRGYVFGSLIWEDGKHHVRSPIVVNLG 736

BLAST of Lcy11g016400 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 857.1 bits (2213), Expect = 1.0e-248
Identity = 424/706 (60.06%), Postives = 527/706 (74.65%), Query Frame = 0

Query: 16  QTAKEAIFYSYNRHINGFAAMLDQKAAEDLAKHPAVASINENKGRKLHTTSSWNFLGVEH 75
           + A +AIFYSY +HINGFAA LD   A +++KHP V S+  NK  KLHTT SW+FLG+EH
Sbjct: 69  ERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEH 128

Query: 76  DDGIPANSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPIPTRWRGSCEG--GSKFH 135
           +  +P++SIW  A FGE TII NLDTGVWPESKSF DEG GPIP+RW+G C+    + FH
Sbjct: 129 NSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFH 188

Query: 136 CNSRKLIGARYFNKGYAAYVGSLNASFETARDHDGHGTHTLSTAGGNFISGASVFGNGNG 195
           CN RKLIGARYFNKGYAA VG LN+SF++ RD DGHG+HTLSTA G+F+ G S+FG GNG
Sbjct: 189 CN-RKLIGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNG 248

Query: 196 TAKGGSPKARVVAYKVCWPKIDSSGGCFDADILAAIEAAITDGVDVLSISLGGDSKDFFD 255
           TAKGGSP+ARV AYKVCWP +     C+DAD+LAA +AAI DG DV+S+SLGG+   FF+
Sbjct: 249 TAKGGSPRARVAAYKVCWPPV-KGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFN 308

Query: 256 DVNAIGAFHAVQHGIIVVCSAGNSGPAPGTVENVAPWLVTVGASTINRQFTSYVALGNKT 315
           D  AIG+FHA +  I+VVCSAGNSGPA  TV NVAPW +TVGAST++R+F S + LGN  
Sbjct: 309 DSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGK 368

Query: 316 HIKGASLSDKILPSQKFYPLINSVDAKASSVSNNNAQLCEEGSLDPKKVKGKILVCLRGE 375
           H KG SLS   LP  KFYP++ SV+AKA + S  +AQLC+ GSLDP K KGKILVCLRG+
Sbjct: 369 HYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQ 428

Query: 376 NGRVDKGFVAAQAGAVGMILANSEQNGDELTADAHLLPASHVSYTDGRSVYQYINSTKIP 435
           NGRV+KG   A  G +GM+L N+   G++L AD H+LPA+ ++  D  +V +YI+ TK P
Sbjct: 429 NGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKP 488

Query: 436 MAFMTRVRTEVGVKPAPFMAEFSSRGPNEIEASILKPDITAPGVSIIAAFAGDLSPTGED 495
           +A +T  RT++G+KPAP MA FSS+GP+ +   ILKPDITAPGVS+IAA+ G +SPT E 
Sbjct: 489 IAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQ 548

Query: 496 LDTRRIPYMAESGTSMSCPHVSGIVGLLRTLYPKWSPAAIRSAIMTTAETKANDLNPILS 555
            D RR+ + A SGTSMSCPH+SGI GLL+T YP WSPAAIRSAIMTTA    +   PI +
Sbjct: 549 FDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQN 608

Query: 556 SSQVKATPFAYGAGHVNPNRAANPGLVYDLTTKDYLNFLCAHGYNETQLKQFSNATFACS 615
           ++ +KATPF++GAGHV PN A NPGLVYDL  KDYLNFLC+ GYN +Q+  FS   F CS
Sbjct: 609 ATNMKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCS 668

Query: 616 K-TFKVTDLNYPSISIPDLKVGTAKVKRRVKNVGSPGTYVAQVKAPPGVSVSVEPSSLKF 675
                + +LNYPSI++P+L      V R VKNVG P  Y  +V  P GV V+V+P+SL F
Sbjct: 669 SPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNF 728

Query: 676 TGVGEEKSFRVVLKRVSNDYHRGYVFGRLAWTDGKHRVRSPIVVNL 719
           T VGE+K+F+V+L +   +  +GYVFG L W+D KHRVRSPIVV L
Sbjct: 729 TKVGEQKTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of Lcy11g016400 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 847.4 bits (2188), Expect = 8.3e-246
Identity = 428/705 (60.71%), Postives = 524/705 (74.33%), Query Frame = 0

Query: 16  QTAKEAIFYSYNRHINGFAAMLDQKAAEDLAKHPAVASINENKGRKLHTTSSWNFLGVEH 75
           + AKEAIFYSY RHINGFAA+LD+  A ++AKHP V S+  NKGRKLHTT SWNF+ +  
Sbjct: 79  ENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAK 138

Query: 76  DDGIPANSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPIPTRWRGSCEGGSKFHCN 135
           +  +  +S+WN A +GE TII NLDTGVWPESKSFSDEGYG +P RW+G C       CN
Sbjct: 139 NGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCH--KDVPCN 198

Query: 136 SRKLIGARYFNKGYAAYVG-SLNASFETARDHDGHGTHTLSTAGGNFISGASVFGNGNGT 195
            RKLIGARYFNKGY AY G   NAS+ET RDHDGHG+HTLSTA GNF+ GA+VFG GNGT
Sbjct: 199 -RKLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGT 258

Query: 196 AKGGSPKARVVAYKVCWPKIDSSGGCFDADILAAIEAAITDGVDVLSISLGGDSKDFFDD 255
           A GGSPKARV AYKVCWP +D +  CFDADILAAIEAAI DGVDVLS S+GGD+ D+  D
Sbjct: 259 ASGGSPKARVAAYKVCWPPVDGA-ECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSD 318

Query: 256 VNAIGAFHAVQHGIIVVCSAGNSGPAPGTVENVAPWLVTVGASTINRQFTSYVALGNKTH 315
             AIG+FHAV++G+ VVCSAGNSGP  GTV NVAPW++TVGAS+++R+F ++V L N   
Sbjct: 319 GIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQS 378

Query: 316 IKGASLSDKILPSQKFYPLINSVDAKASSVSNNNAQLCEEGSLDPKKVKGKILVCLRGEN 375
            KG SLS K LP +K Y LI++ DA  ++ +  +A LC++GSLDPKKVKGKILVCLRG+N
Sbjct: 379 FKGTSLS-KPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDN 438

Query: 376 GRVDKGFVAAQAGAVGMILANSEQNGDELTADAHLLPASHVSYTDGRSVYQYINSTKIPM 435
            RVDKG  AA AGA GM+L N + +G+E+ +DAH+LPAS + Y DG +++ Y++STK P 
Sbjct: 439 ARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPK 498

Query: 436 AFMTRVRTEVGVKPAPFMAEFSSRGPNEIEASILKPDITAPGVSIIAAFAGDLSPTGEDL 495
            ++      +  KPAPFMA FSSRGPN I   ILKPDITAPGV+IIAAF     PT  D 
Sbjct: 499 GYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDS 558

Query: 496 DTRRIPYMAESGTSMSCPHVSGIVGLLRTLYPKWSPAAIRSAIMTTAETKANDLNPILSS 555
           D RR P+  ESGTSMSCPH+SG+VGLL+TL+P WSPAAIRSAIMTT+ T+ N   P++  
Sbjct: 559 DNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDE 618

Query: 556 SQVKATPFAYGAGHVNPNRAANPGLVYDLTTKDYLNFLCAHGYNETQLKQFS-NATFACS 615
           S  KA PF+YG+GHV PN+AA+PGLVYDLTT DYL+FLCA GYN T ++ F+ +  + C 
Sbjct: 619 SFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCR 678

Query: 616 KTFKVTDLNYPSISIPDLKVGTAKVKRRVKNVGSPGTYVAQVKAPPGVSVSVEPSSLKFT 675
           +   + D NYPSI++P+L  G+  V R++KNVG P TY A+ + P GV VSVEP  L F 
Sbjct: 679 QGANLLDFNYPSITVPNL-TGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFN 738

Query: 676 GVGEEKSFRVVLKRVSNDYHRGYVFGRLAWTDGKHRVRSPIVVNL 719
             GE K F++ L+ +      GYVFG L WTD  H VRSPIVV L
Sbjct: 739 KTGEVKIFQMTLRPLPVT-PSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of Lcy11g016400 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 625.2 bits (1611), Expect = 6.7e-179
Identity = 339/704 (48.15%), Postives = 453/704 (64.35%), Query Frame = 0

Query: 22  IFYSYNRHINGFAAMLDQKAAEDLAKHPAVASINENKGRKLHTTSSWNFLGVEHDDGIPA 81
           + Y+Y   I+GF+  L Q+ A+ L   P V S+      +LHTT +  FLG++       
Sbjct: 65  LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTA--- 124

Query: 82  NSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPIPTRWRGSCEGGSKFHCN--SRKL 141
             ++  A      ++G LDTGVWPESKS+SDEG+GPIP+ W+G CE G+ F  +  +RKL
Sbjct: 125 -DLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKL 184

Query: 142 IGARYFNKGYAAYVGSLNASFE--TARDHDGHGTHTLSTAGGNFISGASVFGNGNGTAKG 201
           IGAR+F +GY + +G ++ S E  + RD DGHGTHT STA G+ + GAS+ G  +GTA+G
Sbjct: 185 IGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARG 244

Query: 202 GSPKARVVAYKVCWPKIDSSGGCFDADILAAIEAAITDGVDVLSISLGGDSKDFFDDVNA 261
            +P+ARV  YKVCW      GGCF +DILAAI+ AI D V+VLS+SLGG   D++ D  A
Sbjct: 245 MAPRARVAVYKVCW-----LGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVA 304

Query: 262 IGAFHAVQHGIIVVCSAGNSGPAPGTVENVAPWLVTVGASTINRQFTSYVALGNKTHIKG 321
           IGAF A++ GI+V CSAGN+GP+  ++ NVAPW+ TVGA T++R F +   LGN  +  G
Sbjct: 305 IGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTG 364

Query: 322 ASLSDKILPSQKFYPLINSVDAKASSVSNNNAQLCEEGSLDPKKVKGKILVCLRGENGRV 381
            SL        K  P I +    AS+ +N N  LC  G+L P+KVKGKI++C RG N RV
Sbjct: 365 VSLFKGEALPDKLLPFIYA--GNASNATNGN--LCMTGTLIPEKVKGKIVMCDRGINARV 424

Query: 382 DKGFVAAQAGAVGMILANSEQNGDELTADAHLLPASHVSYTDGRSVYQYINSTKIPMAFM 441
            KG V   AG VGMILAN+  NG+EL ADAHLLPA+ V    G  +  Y+ +   P A +
Sbjct: 425 QKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASI 484

Query: 442 TRVRTEVGVKPAPFMAEFSSRGPNEIEASILKPDITAPGVSIIAAFAGDLSPTGEDLDTR 501
           + + T VGVKP+P +A FSSRGPN I  +ILKPD+ APGV+I+AA+ G   PTG   D+R
Sbjct: 485 SILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSR 544

Query: 502 RIPYMAESGTSMSCPHVSGIVGLLRTLYPKWSPAAIRSAIMTTAETKANDLNPILSSSQV 561
           R+ +   SGTSMSCPHVSG+  LL++++P+WSPAAIRSA+MTTA     D  P+L  +  
Sbjct: 545 RVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATG 604

Query: 562 K-ATPFAYGAGHVNPNRAANPGLVYDLTTKDYLNFLCAHGYNETQLKQFSNATFAC--SK 621
           K +TPF +GAGHV+P  A NPGL+YDLTT+DYL FLCA  Y   Q++  S   + C  SK
Sbjct: 605 KPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSK 664

Query: 622 TFKVTDLNYPSISIPDLKVGTAKVKRRVKNVGSPGTYVAQVKA-PPGVSVSVEPSSLKFT 681
           ++ V DLNYPS ++    VG  K  R V +VG  GTY  +V +   GV +SVEP+ L F 
Sbjct: 665 SYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFK 724

Query: 682 GVGEEKSFRVVLKRVSNDYHRGYVFGRLAWTDGKHRVRSPIVVN 718
              E+KS+ V     S+       FG + W+DGKH V SP+ ++
Sbjct: 725 EANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of Lcy11g016400 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 568.9 bits (1465), Expect = 5.7e-162
Identity = 327/726 (45.04%), Postives = 451/726 (62.12%), Query Frame = 0

Query: 13  HKTQTAKEA-IFYSYNRHINGFAAMLDQKAAEDLAKHPAVASINENKGRKLHTTSSWNFL 72
           + T+ A+E+ I + Y+   +GF+A++    A++L  HPAV ++ E++ R+LHTT S  FL
Sbjct: 48  YSTEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFL 107

Query: 73  GVEHDDGIPANSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPIPTRWRGSCEGGSK 132
           G+++  G     +W+ + +G   IIG  DTG+WPE +SFSD   GPIP RWRG CE G++
Sbjct: 108 GLQNQKG-----LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGAR 167

Query: 133 F---HCNSRKLIGARYFNKG-YAAYVGSLNASFE--TARDHDGHGTHTLSTAGGNFISGA 192
           F   +CN RK+IGAR+F KG  AA +G +N + E  + RD DGHGTHT STA G     A
Sbjct: 168 FSPRNCN-RKIIGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKA 227

Query: 193 SVFGNGNGTAKGGSPKARVVAYKVCWPKIDSSGGCFDADILAAIEAAITDGVDVLSISLG 252
           S+ G  +G AKG +PKAR+ AYKVCW       GC D+DILAA +AA+ DGVDV+SIS+G
Sbjct: 228 SMSGYASGVAKGVAPKARIAAYKVCW----KDSGCLDSDILAAFDAAVRDGVDVISISIG 287

Query: 253 GD---SKDFFDDVNAIGAFHAVQHGIIVVCSAGNSGPAPGTVENVAPWLVTVGASTINRQ 312
           G    +  ++ D  AIG++ A   GI V  SAGN GP   +V N+APW+ TVGASTI+R 
Sbjct: 288 GGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRN 347

Query: 313 FTSYVALGNKTHIKGASLSDKILPSQKFYPLINSVDAKASSVSNNNAQLCEEGSLDPKKV 372
           F +   LG+   ++G SL   +  + + +P++    +  SS     A LC E +LDPK+V
Sbjct: 348 FPADAILGDGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSS-----ASLCMENTLDPKQV 407

Query: 373 KGKILVCLRGENGRVDKGFVAAQAGAVGMILANSEQNGDELTADAHLLPASHVSYTDGRS 432
           +GKI++C RG + RV KG V  +AG VGMILAN   NG+ L  DAHL+PA  V   +G  
Sbjct: 408 RGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDR 467

Query: 433 VYQYINSTKIPMAFMTRVRTEVGVKPAPFMAEFSSRGPNEIEASILKPDITAPGVSIIAA 492
           +  Y +S   P+A +    T VG+KPAP +A FS RGPN +   ILKPD+ APGV+I+AA
Sbjct: 468 IKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAA 527

Query: 493 FAGDLSPTGEDLDTRRIPYMAESGTSMSCPHVSGIVGLLRTLYPKWSPAAIRSAIMTTAE 552
           +   + PTG   D R+  +   SGTSM+CPHVSG   LL++ +P WSPA IRSA+MTT  
Sbjct: 528 WTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTN 587

Query: 553 TKANDLNPILSSSQVK-ATPFAYGAGHVNPNRAANPGLVYDLTTKDYLNFLCAHGYNETQ 612
              N    ++  S  K ATP+ YG+GH+N  RA NPGLVYD+T  DY+ FLC+ GY    
Sbjct: 588 LVDNSNRSLIDESTGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKT 647

Query: 613 LKQFSNATFACSKTFKVT--DLNYPSIS--IPDLKVG--TAKVKRRVKNVG-SPGTYVAQ 672
           ++  +     C  T K +  +LNYPSI+   P  + G  +  V R   NVG +   Y A+
Sbjct: 648 IQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRAR 707

Query: 673 VKAPPGVSVSVEPSSLKFTGVGEEKSFRVVLKRVSNDY---HRGYVFGRLAWTD-GKHRV 717
           +++P GV+V+V+P  L FT   + +S+ V +   + +      G VFG + W D GKH V
Sbjct: 708 IESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVV 758

BLAST of Lcy11g016400 vs. TAIR 10
Match: AT5G45650.1 (subtilase family protein )

HSP 1 Score: 558.1 bits (1437), Expect = 1.0e-158
Identity = 327/742 (44.07%), Postives = 446/742 (60.11%), Query Frame = 0

Query: 16  QTAKEAIFYSYNRHINGFAAMLDQKAAEDLAKHPAVASINENKGRK--LHTTSSWNFLGV 75
           + A+ ++ YSY   INGFAA L    A  L K   V S+ ++  RK   HTT SW F+G+
Sbjct: 59  EDARASLLYSYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGL 118

Query: 76  EH---DDGIP------------ANSIWNLASFGESTIIGNLDTGVWPESKSFSDEGYGPI 135
           E    D  +P              +    A  G+  I+G LD+GVWPESKSF+D+G GP+
Sbjct: 119 EEEETDSDVPRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDSGVWPESKSFNDKGMGPV 178

Query: 136 PTRWRGSCEGGSKF---HCNSRKLIGARYFNKGYAAYVGSLNAS----FETARDHDGHGT 195
           P  W+G C+ G  F   HCN RK+IGARY+ KGY  Y G+ NA+    F + RD DGHG+
Sbjct: 179 PKSWKGICQTGVAFNSSHCN-RKIIGARYYVKGYERYYGAFNATANKDFLSPRDPDGHGS 238

Query: 196 HTLSTAGGNFISGASVFGN-GNGTAKGGSPKARVVAYKVCWPKIDS----SGGCFDADIL 255
           HT STA G  + GAS  G    G+A GG+P AR+  YK CW K ++       C + D+L
Sbjct: 239 HTASTAVGRRVLGASALGGFAKGSASGGAPLARLAIYKACWAKPNAEKVEGNICLEEDML 298

Query: 256 AAIEAAITDGVDVLSISLG-GDSKDFFDDVNAIGAFHAVQHGIIVVCSAGNSGPAPGTVE 315
           AAI+ AI DGV V+SIS+G  +   F  D  A+GA HAV+  I+V  SAGNSGP PGT+ 
Sbjct: 299 AAIDDAIADGVHVISISIGTTEPFPFTQDGIAMGALHAVKRNIVVAASAGNSGPKPGTLS 358

Query: 316 NVAPWLVTVGASTINRQFTSYVALGNKTHIKGASLSDKILPSQKFYPLINSVDAKASSVS 375
           N+APW++TVGAST++R F   + LGN   IK  S++       KF PL+ + +     ++
Sbjct: 359 NLAPWIITVGASTLDRAFVGGLVLGNGYTIKTDSIT--AFKMDKFAPLVYASNVVVPGIA 418

Query: 376 NNNAQLCEEGSLDPKKVKGKILVCLRGENGRVDKGFVAAQAGAVGMILANSEQNGDELTA 435
            N    C   SL P+ V GK+++CLRG   R+ KG    +AG  GMIL N   NG+E+ +
Sbjct: 419 LNETSQCLPNSLKPELVSGKVVLCLRGAGSRIGKGMEVKRAGGAGMILGNIAANGNEVPS 478

Query: 436 DAHLLPASHVSYTDGRSVYQYINSTKIPMAFMTRVRTEVGVKPAPFMAEFSSRGPNEIEA 495
           D+H +P + V+ T    + +YI + K P AF+   +T    + AP M  FSSRGPN ++ 
Sbjct: 479 DSHFVPTAGVTPTVVDKILEYIKTDKNPKAFIKPGKTVYKYQAAPSMTGFSSRGPNVVDP 538

Query: 496 SILKPDITAPGVSIIAAFAGDLSPTGEDLDTRRIPYMAESGTSMSCPHVSGIVGLLRTLY 555
           +ILKPDITAPG+ I+AA++G  SP+   +D R   Y   SGTSMSCPHV+G + LL+ ++
Sbjct: 539 NILKPDITAPGLYILAAWSGADSPSKMSVDQRVAGYNIYSGTSMSCPHVAGAIALLKAIH 598

Query: 556 PKWSPAAIRSAIMTTAETKANDLNPILSSSQVKATPFAYGAGHVNPNRAANPGLVYDLTT 615
           PKWS AAIRSA+MTTA    +   PI  ++ + A PFA G+GH  P +AA+PGLVYD + 
Sbjct: 599 PKWSSAAIRSALMTTAWMTNDKKKPIQDTTGLPANPFALGSGHFRPTKAADPGLVYDASY 658

Query: 616 KDYLNFLCAHGYNETQLKQFSNATFAC-SKTFKVTDLNYPSISIPDLKVGTAKVKRRVKN 675
           + YL + C+   N T +    + TF C SK     + NYPSI++P+LK  T  VKR V N
Sbjct: 659 RAYLLYGCS--VNITNI----DPTFKCPSKIPPGYNHNYPSIAVPNLK-KTVTVKRTVTN 718

Query: 676 VG---SPGTYVAQVKAPPGVSVSVEPSSLKFTGVGEEKSFRVVLK----RVSNDYHRG-Y 719
           VG   S  TY+  VK P G+SV   P+ L F  +G+++ F++V+K    +V N   +G Y
Sbjct: 719 VGTGNSTSTYLFSVKPPSGISVKAIPNILSFNRIGQKQRFKIVIKPLKNQVMNATEKGQY 778