Lcy11g015730 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy11g015730
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Ribonuclease H
Location	Chr11: 37461731 .. 37462455 (+)
RNA-Seq Expression	Lcy11g015730
Synteny	Lcy11g015730

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCAACGTTTAATCAATTTAAGGGCAATTTAAGAGCTTCAAATTAAATAAACCATAAACATACATTTTTTTTATGTCTAAAACTTTAAACTTTCAAAATTACAAAAATCATGAAATAAAGATTAAAAGAATCATAATGATTAGAATCTTACCAGTGAAGAGAAGACGCTGCGACACGAAGAGGAGCAGACGAATCGGCGATCATCTGCGAACTACTCTGGCACCGATCAGGATGAGACGCGTCGATTATTCAAGTGGCTAAAACCTCCGGACGGCACCTGGAATCTCAATAGTGATGCTTCCTGGAGCGAAGAGAAGCAGATGGGAGGCGTCGGCTGGATCCTTAGACGCTGGGATGGGGCGATTATTGCAGCAAGATATCAGGTTGTTCACCGGCAATGGAAAATTGGTTGGCTAGAAGCCCTAGCTGTGTTGAAAGGGTTGAAAAACATTCCCCAAGACTCCCCTCAGGTGTTTATCGAGCTTGACTCAGTGCAGGTGGTAAACTTACTCATGGGGAAGGAGGAGGGTGTCACTCAAATCTCGATGTTCATTGATGAAGCTCAACAAATAATTCGCCTGAAGAACATTCAATCGATCCAACATGTTGCAAGACGCCACAATCACATGGCCCACCAGTGGGCCCGTTTAGCTTGGTCCAAAAACCAGTCTGAAACTTTCGAGGGTCCATTTCAAAGTTTCCATTTCCTTTATGGCTTTTAG

mRNA sequence

ATGGCCAACAATCTTACCAGTGAAGAGAAGACGCTGCGACACGAAGAGGAGCAGACGAATCGGCGATCATCTGCGAACTACTCTGGCACCGATCAGGATGAGACGCGTCGATTATTCAAGTGGCTAAAACCTCCGGACGGCACCTGGAATCTCAATAGTGATGCTTCCTGGAGCGAAGAGAAGCAGATGGGAGGCGTCGGCTGGATCCTTAGACGCTGGGATGGGGCGATTATTGCAGCAAGATATCAGGTTGTTCACCGGCAATGGAAAATTGGTTGGCTAGAAGCCCTAGCTGTGTTGAAAGGGTTGAAAAACATTCCCCAAGACTCCCCTCAGGTGTTTATCGAGCTTGACTCAGTGCAGGTGGTAAACTTACTCATGGGGAAGGAGGAGGGTGTCACTCAAATCTCGATGTTCATTGATGAAGCTCAACAAATAATTCGCCTGAAGAACATTCAATCGATCCAACATGTTGCAAGACGCCACAATCACATGGCCCACCAGTGGGCCCGTTTAGCTTGGTCCAAAAACCAGTCTGAAACTTTCGAGGGTCCATTTCAAAGTTTCCATTTCCTTTATGGCTTTTAG

Coding sequence (CDS)

ATGGCCAACAATCTTACCAGTGAAGAGAAGACGCTGCGACACGAAGAGGAGCAGACGAATCGGCGATCATCTGCGAACTACTCTGGCACCGATCAGGATGAGACGCGTCGATTATTCAAGTGGCTAAAACCTCCGGACGGCACCTGGAATCTCAATAGTGATGCTTCCTGGAGCGAAGAGAAGCAGATGGGAGGCGTCGGCTGGATCCTTAGACGCTGGGATGGGGCGATTATTGCAGCAAGATATCAGGTTGTTCACCGGCAATGGAAAATTGGTTGGCTAGAAGCCCTAGCTGTGTTGAAAGGGTTGAAAAACATTCCCCAAGACTCCCCTCAGGTGTTTATCGAGCTTGACTCAGTGCAGGTGGTAAACTTACTCATGGGGAAGGAGGAGGGTGTCACTCAAATCTCGATGTTCATTGATGAAGCTCAACAAATAATTCGCCTGAAGAACATTCAATCGATCCAACATGTTGCAAGACGCCACAATCACATGGCCCACCAGTGGGCCCGTTTAGCTTGGTCCAAAAACCAGTCTGAAACTTTCGAGGGTCCATTTCAAAGTTTCCATTTCCTTTATGGCTTTTAG

Protein sequence

MANNLTSEEKTLRHEEEQTNRRSSANYSGTDQDETRRLFKWLKPPDGTWNLNSDASWSEEKQMGGVGWILRRWDGAIIAARYQVVHRQWKIGWLEALAVLKGLKNIPQDSPQVFIELDSVQVVNLLMGKEEGVTQISMFIDEAQQIIRLKNIQSIQHVARRHNHMAHQWARLAWSKNQSETFEGPFQSFHFLYGF

Homology

[Close]

BLAST of Lcy11g015730 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 3.7e-15
Identity = 45/142 (31.69%), Postives = 84/142 (59.15%), Query Frame = 0


Query: 40  KWLKPPDGTWNLNSDASWSEEKQMGGVGWILRRWDGAIIAARYQVVHRQWKIGWLEALAV 99
           +W  P   +W LN++A+W  +   GG+GWILR   G +I A  +++  +  I +LE +A+
Sbjct: 78  QWKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAI 137

Query: 100 LKGLKNIPQDSPQ-VFIELDSVQVVNLLMGKEEGVTQISMFIDEAQQIIRLKNIQSIQHV 159
            +GL+ I Q+  + + +E DS++ ++LL  + +  T+I   ++E  Q+++   I S++H+
Sbjct: 138 CEGLRAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHI 197

Query: 160 ARRHNHMAHQWARLAWSKNQSE 181
           +R  N +AH  AR A   +  E
Sbjct: 198 SREANKVAHGLARRAMENDLRE 219

[Close]

BLAST of Lcy11g015730 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 8.3e-15
Identity = 46/150 (30.67%), Postives = 83/150 (55.33%), Query Frame = 0


Query: 40  KWLKPPDGTWNLNSDASWSEEKQMGGVGWILRRWDGAIIAARYQVVHRQWKIGWLEALAV 99
           +W  P   +W LN+DA+W  +   GG+GWILR   G +I A  +++  +  I +LE +A+
Sbjct: 78  RWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAI 137

Query: 100 LKGLKNIPQDSPQ---------VFIELDSVQVVNLLMGKEEGVTQISMFIDEAQQIIRLK 159
            +GL+ I Q+  +         + +E DS++ ++LL  + +  T+I   ++E  Q++   
Sbjct: 138 CEGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDM 197

Query: 160 NIQSIQHVARRHNHMAHQWARLAWSKNQSE 181
            I S++H++R  N +AH  AR A   +  E
Sbjct: 198 KIVSMRHISREANKVAHDLARRAMENDLRE 227

[Close]

BLAST of Lcy11g015730 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 8.3e-15
Identity = 51/136 (37.50%), Postives = 72/136 (52.94%), Query Frame = 0


Query: 40  KWLKPPDGTWNLNSDASWSEEKQMGGVGWILRRWDGAIIAARYQVVHRQWKIGWLEALAV 99
           KW  PP   W LN+DASWS+    GG+GWI+R WDG I+ A  + V     +  LEA A+
Sbjct: 70  KWEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAI 129

Query: 100 LKGLKNIPQDS--PQVFIELDSVQVVNLLMGKEEGVTQISMFIDEAQQIIRLKNIQSIQH 159
           L+GL+N+        + IE DS +V +LL  K E +T+    ++E   +     I +   
Sbjct: 130 LEGLRNLTNLGVLRPLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAK 189

Query: 160 VARRHNHMAHQWARLA 174
           V R  N  AH  A+ A
Sbjct: 190 VGRETNGCAHSLAQRA 205

[Close]

BLAST of Lcy11g015730 vs. ExPASy TrEMBL
Match: A0A6J1CQG0 (uncharacterized protein LOC111013216 OS=Momordica charantia OX=3673 GN=LOC111013216 PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 2.5e-11
Identity = 37/97 (38.14%), Postives = 62/97 (63.92%), Query Frame = 0


Query: 40  KWLKPPDGTWNLNSDASWSEEKQMGGVGWILRRWDGAIIAARYQVVHRQWKIGWLEALAV 99
           +W  PP   W LN+DASWSEE+++GG+GWIL    G I+ A    +  + +I  LE + +
Sbjct: 104 RWSAPPTNCWKLNTDASWSEEREVGGIGWILCDCRGEIVLAGNCKIREKKEINALELMTI 163

Query: 100 LKGLK--NIPQDSPQVFIELDSVQVVNLLMGKEEGVT 135
           ++GL+  N+   SP +++E DSV+V+ L+  ++  +T
Sbjct: 164 IRGLQFINMQSRSP-IYLESDSVEVIRLMKKEDVDLT 199

[Close]

BLAST of Lcy11g015730 vs. ExPASy TrEMBL
Match: A0A7J6ERF5 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_020551 PE=4 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 3.3e-11
Identity = 48/159 (30.19%), Postives = 79/159 (49.69%), Query Frame = 0


Query: 30  TDQDETRRLFKWLKPPDGTWNLNSDASWSEEKQMGGVGWILRRWDGAIIAARYQVVHRQW 89
           T   E  +   W++PP G   +N DA+ +      G G+I R WDG ++ A     +R  
Sbjct: 215 TTNMELSKTTHWIRPPAGYIAVNCDAAMNSGAAGIGTGFIWRNWDGKLLIAGMNYSNRCC 274

Query: 90  KIGWLEALAVLKGLKNIPQ--DSPQVFIELDSVQVVNLLMGKEEGVTQISMFIDEAQQII 149
            +   EA A+L+ L N+P   DSP + I+ D   +V+ +M ++  +T ++  I + +  +
Sbjct: 275 SVDMAEAWAILEALNNMPNTTDSP-MEIQSDCKHLVDKIMNQDNHLTAVNTIIHQIRMQM 334

Query: 150 RLKNIQSIQHVARRHNHMAHQWARLAWSKNQSETFEGPF 187
              N  SI HV R +N  AH  AR   +   ++ F  PF
Sbjct: 335 EKLNCNSIVHVNRNNNECAHMLARKCLATKTTQIFTHPF 372

[Close]

BLAST of Lcy11g015730 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 91.7 bits (226), Expect = 7.7e-15
Identity = 45/142 (31.69%), Postives = 84/142 (59.15%), Query Frame = 0


Query: 40  KWLKPPDGTWNLNSDASWSEEKQMGGVGWILRRWDGAIIAARYQVVHRQWKIGWLEALAV 99
           +W  P   +W LN++A+W  +   GG+GWILR   G +I A  +++  +  I +LE +A+
Sbjct: 78  QWKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAI 137

Query: 100 LKGLKNIPQDSPQ-VFIELDSVQVVNLLMGKEEGVTQISMFIDEAQQIIRLKNIQSIQHV 159
            +GL+ I Q+  + + +E DS++ ++LL  + +  T+I   ++E  Q+++   I S++H+
Sbjct: 138 CEGLRAIRQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHI 197

Query: 160 ARRHNHMAHQWARLAWSKNQSE 181
           +R  N +AH  AR A   +  E
Sbjct: 198 SREANKVAHGLARRAMENDLRE 219

[Close]

BLAST of Lcy11g015730 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 90.5 bits (223), Expect = 1.7e-14
Identity = 51/136 (37.50%), Postives = 72/136 (52.94%), Query Frame = 0


Query: 40  KWLKPPDGTWNLNSDASWSEEKQMGGVGWILRRWDGAIIAARYQVVHRQWKIGWLEALAV 99
           KW  PP   W LN+DASWS+    GG+GWI+R WDG I+ A  + V     +  LEA A+
Sbjct: 70  KWEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAI 129

Query: 100 LKGLKNIPQDS--PQVFIELDSVQVVNLLMGKEEGVTQISMFIDEAQQIIRLKNIQSIQH 159
           L+GL+N+        + IE DS +V +LL  K E +T+    ++E   +     I +   
Sbjct: 130 LEGLRNLTNLGVLRPLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAK 189

Query: 160 VARRHNHMAHQWARLA 174
           V R  N  AH  A+ A
Sbjct: 190 VGRETNGCAHSLAQRA 205

[Close]

BLAST of Lcy11g015730 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 90.5 bits (223), Expect = 1.7e-14
Identity = 46/150 (30.67%), Postives = 83/150 (55.33%), Query Frame = 0


Query: 40  KWLKPPDGTWNLNSDASWSEEKQMGGVGWILRRWDGAIIAARYQVVHRQWKIGWLEALAV 99
           +W  P   +W LN+DA+W  +   GG+GWILR   G +I A  +++  +  I +LE +A+
Sbjct: 78  RWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAI 137

Query: 100 LKGLKNIPQDSPQ---------VFIELDSVQVVNLLMGKEEGVTQISMFIDEAQQIIRLK 159
            +GL+ I Q+  +         + +E DS++ ++LL  + +  T+I   ++E  Q++   
Sbjct: 138 CEGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDM 197

Query: 160 NIQSIQHVARRHNHMAHQWARLAWSKNQSE 181
            I S++H++R  N +AH  AR A   +  E
Sbjct: 198 KIVSMRHISREANKVAHDLARRAMENDLRE 227

[Close]

BLAST of Lcy11g015730 vs. NCBI nr
Match: XP_015388122.1 (uncharacterized protein LOC107178012 [Citrus sinensis] >XP_024041306.1 uncharacterized protein LOC112098894 [Citrus clementina])

HSP 1 Score: 79.7 bits (195), Expect = 3.0e-11
Identity = 51/157 (32.48%), Postives = 83/157 (52.87%), Query Frame = 0


Query: 31  DQDETRRLFKWLKPPDGTWNLNSDASWSEEKQMGGVGWILRRWDGAIIAARYQVVHRQWK 90
           ++ +  R   W  PP G + +N DA+ + + QM G+G I+R   G ++AA  Q    Q  
Sbjct: 68  EKQQRNRKQVWNPPPSGWFKINVDAAMNVKDQMAGLGVIIRNSKGEVVAAAVQKSCFQLS 127

Query: 91  IGWLEALAVLKGLKNIPQ-DSPQVFIELDSVQVVNLLMGKEEGVTQISMFIDEAQQIIRL 150
              +EA AV+ G+K+  + +   + IE DS +VV+L + K+  +T+ S  I + Q+ I+ 
Sbjct: 128 STHMEAEAVILGIKSAQRAEFSLMIIETDSQEVVDLTLSKKVSITETSWIIADIQESIQS 187

Query: 151 KNIQSIQHVARRHNHMAHQWARLAWSKNQSETFEGPF 187
            N  SIQ+  R  N  AH  A+ A     S  ++G F
Sbjct: 188 SNQFSIQYTPRECNVTAHDLAKKALEFENSVFWDGSF 224

[Close]

BLAST of Lcy11g015730 vs. NCBI nr
Match: XP_022143317.1 (uncharacterized protein LOC111013216 [Momordica charantia])

HSP 1 Score: 79.0 bits (193), Expect = 5.2e-11
Identity = 37/97 (38.14%), Postives = 62/97 (63.92%), Query Frame = 0


Query: 40  KWLKPPDGTWNLNSDASWSEEKQMGGVGWILRRWDGAIIAARYQVVHRQWKIGWLEALAV 99
           +W  PP   W LN+DASWSEE+++GG+GWIL    G I+ A    +  + +I  LE + +
Sbjct: 104 RWSAPPTNCWKLNTDASWSEEREVGGIGWILCDCRGEIVLAGNCKIREKKEINALELMTI 163

Query: 100 LKGLK--NIPQDSPQVFIELDSVQVVNLLMGKEEGVT 135
           ++GL+  N+   SP +++E DSV+V+ L+  ++  +T
Sbjct: 164 IRGLQFINMQSRSP-IYLESDSVEVIRLMKKEDVDLT 199

[Close]

BLAST of Lcy11g015730 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 43.5 bits (101), Expect = 2.2e-04
Identity = 38/157 (24.20%), Postives = 68/157 (43.31%), Query Frame = 0


Query: 16  EEQTNRRSSANYSGTDQDETRRLFKWLKPPDGTWNLNSDASWSEEKQMGGVGWILRRWDG 75
           EE + RR     +   Q E     +W  PP      N+DA+W  E    G+GWILR   G
Sbjct: 112 EEWSTRRELEGKASGPQVERNLSVQWKAPPYQWVKCNTDATWQLENPRCGIGWILRNESG 171

Query: 76  AIIAARYQVVHRQWKIGWLEALAVLKGLKNIPQ-DSPQVFIELDSVQVVNLLMGKEEGVT 135
            ++    + + R   +   E  A+   +  + + +  ++  E D+  +VNLL   +   T
Sbjct: 172 GVLWMGARALPRTKNVLEAELEALRWAVLTMSRFNYKRIIFESDAQALVNLLNSDDFWPT 231

Query: 136 QISMFIDEAQQIIRLKNIQSIQHVARRHNHMAHQWAR 172
            +   +++ QQ++        +   R  N +A + AR
Sbjct: 232 -LQPALEDIQQLLHHFEEVKFEFTPRGGNKVADRIAR 267

The following BLAST results are available for this feature:

BLAST of Lcy11g015730 vs.


Match Name	E-value	Identity	Description

BLAST of Lcy11g015730 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CP26	3.7e-15	31.69	uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1DSV1	8.3e-15	30.67	uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1DNV9	8.3e-15	37.50	uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1CQG0	2.5e-11	38.14	uncharacterized protein LOC111013216 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A7J6ERF5	3.3e-11	30.19	Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_020551 PE=4 SV=1	[more]

BLAST of Lcy11g015730 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022143535.1	7.7e-15	31.69	uncharacterized protein LOC111013412 [Momordica charantia]	[more]
XP_022155262.1	1.7e-14	37.50	uncharacterized protein LOC111022403 [Momordica charantia]	[more]
XP_022156777.1	1.7e-14	30.67	uncharacterized protein LOC111023608 [Momordica charantia]	[more]
XP_015388122.1	3.0e-11	32.48	uncharacterized protein LOC107178012 [Citrus sinensis] >XP_024041306.1 uncharact...	[more]
XP_022143317.1	5.2e-11	38.14	uncharacterized protein LOC111013216 [Momordica charantia]	[more]

BLAST of Lcy11g015730 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G34320.1	2.2e-04	24.20	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 47..175 e-value: 3.0E-11 score: 45.5
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 52..173 e-value: 3.1E-16 score: 59.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..31
None	No IPR available	PANTHER	PTHR47723	OS05G0353850 PROTEIN	coord: 29..183
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 51..171 e-value: 1.32687E-14 score: 65.028
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 48..176

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy11g015730.1	Lcy11g015730.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity