Lcy11g005290 (gene) Sponge gourd (P93075) v1

BLAST of Lcy11g005290 vs. ExPASy Swiss-Prot
Match: Q5IBC5 (Separase OS=Arabidopsis thaliana OX=3702 GN=ESP1 PE=2 SV=1)

HSP 1 Score: 1691.8 bits (4380), Expect = 0.0e+00
Identity = 987/2294 (43.03%), Postives = 1368/2294 (59.63%), Query Frame = 0

Query: 2    ASPSESSLISILETADSKGISSLVSDFLQPFSDVKNPKKCKKSAKSTDDSSTIRSLAKKF 61
            +S  +  L+S+++  D+  + S  SD+L+PFS +        S K  D ++TIR+LAK+F
Sbjct: 3    SSGDDLRLLSLIDVGDN--VFSSFSDYLKPFSTLST------SRKKQDRATTIRALAKQF 62

Query: 62   LAFLNRALSILPKRLSEPSKLGNDLELALEFFEVYKLCLGCLESLTSQLSCKPYTVDVQR 121
            L FLN+++S+LPKRLS  +      E AL+ F  Y+LCL CLE +++QL+CKP+TV  QR
Sbjct: 63   LPFLNKSISLLPKRLSVANSDKEARESALDLFRAYELCLDCLELVSAQLACKPHTVQSQR 122

Query: 122  VRMVHCMEDWGLFKDAEAEGFGILERLREIDRKSKAGKLDCRVIHDGDKGGGDKDFCLLF 181
            +RM+HC++ WGL+++   E F +LE+LR  D KS+  +L   V        GD +  L+ 
Sbjct: 123  LRMIHCLDVWGLYENVYTEAFKVLEKLRGSDSKSRKSRLLPEV------QDGDAEMALVV 182

Query: 182  AEVVVTIVKCTASGRSKESGDYRRVLGLVEEVRPWFRLLDAKVSEKTQRALVTYLGKCTI 241
             + V  I +  A  +  +   YR+VL L+EEV  W R+LDAKV EK  RA+VT +GKC +
Sbjct: 183  VDAVAAIFRAVAMSQQLDDKRYRKVLLLLEEVGGWLRVLDAKVYEKLHRAMVTSMGKCAV 242

Query: 242  FLVEEVVCFGESLVSLFCRTTFAEYAKSSM-RDQIYKLARRICSTLFSLQQEQHTSMLIM 301
             LV E   F   LV  FC  T  E+ KS++ +D++YK AR + S LF  +  + +  + +
Sbjct: 243  SLVREAERFNGDLVISFCDLTVKEHYKSALSKDRVYKFAREVLSVLFGFKDRKMS--VTI 302

Query: 302  KILACVLKSITLEWKDETECTVIEFLQLICYCANKCQSESPECCCTFARHLEEMAADFHQ 361
             I   VL+S++ +++DE+   ++EF  L+ YCA+K ++     C   ++ L EMAA F +
Sbjct: 303  DISMSVLRSLSCQFEDESNENLMEFFDLVDYCAHKFRAAGDMYCAKVSKKLNEMAAIFVE 362

Query: 362  ATTPFGMNLRLYAAGLKIF---SKVPRGETHGSA----FSILVDDEDTMQSLVNLNGFLG 421
            A     + LRLY+ GL I    SK+   +   S        + DD+   QSLV+L G + 
Sbjct: 363  AIPQLNLVLRLYSTGLSITVCNSKLGEIKLEDSTDDWKIQAMFDDDARWQSLVSLLGMVD 422

Query: 422  SYFCIGCRKGNESCSNEQKDFVDQPCSHGKSNHECD--VPLLSWREAYLFSYLDAIKFLC 481
            SY       G+E               + K++  C     +  W +     Y+DA+KFLC
Sbjct: 423  SY------SGDEGNQTGSSSIGGHRNYNNKTHDSCKDRNKITCWPQ-----YVDALKFLC 482

Query: 482  QPLAESVNSERKEILAEDK---AASVLYSIQNTLYQSCDVFLFCQRRAYDAKSDGCDENV 541
            QPLA+ + S +++I+ E +   A++ L +I +   Q CD  LF QR   D K D    N 
Sbjct: 483  QPLADFIYSVKRKIVLETEMSCASAHLITIHDAFLQFCDGCLFLQRCTSD-KGDREIANN 542

Query: 542  NMLLSVVMAAFTLSIRTRLEMKRSTDLIKAVISSKWVQPFALKHLFASLNNIGIILYRNK 601
               L+  M AF +S+RT+L+++ S  L++ VI S W+Q   LK+L A+L NIGI+LYRNK
Sbjct: 543  KAFLNAAMGAFIVSLRTQLKLEISAHLVEDVIGSPWIQSQELKYLIATLYNIGIVLYRNK 602

Query: 602  QIREASKALKLCCRASWTCVRHFCVMFDDKAKPSDNEFSENAVLSFVNEVIMRSAFLVDI 661
            ++ +A +ALKLC + SW CV   C MF +++  SDN+ SE+A++ FV E   R AF +DI
Sbjct: 603  ELNKACEALKLCSKVSWRCVELHCHMFVNQSSSSDNDLSEDAIMDFVGEACNRCAFYLDI 662

Query: 662  LYQRGMHKVEKAVTEILKNWSMAEKLFNGLPVPVQLVKQWVKMQCKHHKNADPRKTTPTL 721
            L +    K+ + +  IL+NW  AE L   LP P  +VKQWVK++ + H + D   +  TL
Sbjct: 663  LQKCSRRKIRQNIVHILENWLSAEHLIRRLPGPEAIVKQWVKIERECHTDLDAAGSCTTL 722

Query: 722  NCLLQSSGMLSKAQIGALLEQELVEYAEMSGLYPEFCQSLQMKILSTLLRDVYVTPDHRL 781
              LL SS   SK  IG +LEQEL+ Y  +  L     Q  ++KI   LL+DVYVT D  +
Sbjct: 723  YSLLSSSQKKSKRGIGKILEQELLAYDRVLPLRSNLGQQTRIKIADILLKDVYVTEDMHI 782

Query: 782  EKARVLLKKARVLRFCETICLDNCIQCLSEAISTINETSGGTCSSGVLHSHQLAVAYCLR 841
            E+AR+L+ KAR+ R   T  +  CI  LSEAIS + E   G    G   SH L +AYCLR
Sbjct: 783  ERARILIWKARMTRTSGTEHITECICFLSEAISILGELHHGPNEEGSPSSHMLPIAYCLR 842

Query: 842  ALCTHETVPNSKQVLQDIDSALSVWLEISSLDSLPDDQQLILSEYMLLLLCNTFDLLSIK 901
            A CT E  PNSK+V QDI ++L++WL I SLD   D    + +E ++ LL N  DL+S+K
Sbjct: 843  AFCTQEADPNSKKVFQDISTSLNLWLRILSLD---DSGDSLPTENIIPLLYNMIDLMSVK 902

Query: 902  GCMDYYHNIYSLMIRLFKWKNVQLEKLLAVLWEGRRMSHAICIAPANEIVITQLSEHLGE 961
            GC + +H+IY L+ RLFKWKNV+LE  LA+LWE RR+SHA+C +P ++  I  LSE+  +
Sbjct: 903  GCTELHHHIYQLIFRLFKWKNVKLEVCLAMLWECRRLSHALCPSPISDAFIQTLSENCAD 962

Query: 962  LPKSFNFWTNCLKTLPGMLVGFRQNFSFLCRNNGQSCCEHEKSVQLDVTVDEVKEAASKL 1021
                 +FW +CLK     L+GF+QNF  L   +       E   Q D+T+D++K+AAS+L
Sbjct: 963  KSTCIDFWMDCLKDSKAKLIGFQQNFHDLHNKD-------EGPFQSDITIDDIKDAASEL 1022

Query: 1022 ISHTPACKSSIFLAGYLYYDLCERLISEGCLTEALSCAKEAHRLRSKLFQEKFTYSIEQH 1081
            IS      +S F A YLYYDLCERLIS G L+EALS AKEA+R+R+ +FQ+KF Y+ E+H
Sbjct: 1023 ISSASLSGNSSFAAAYLYYDLCERLISFGKLSEALSYAKEAYRIRTLIFQDKFKYTAEKH 1082

Query: 1082 PEKYCEISHVSQKPAYGIKNLQKNGLVARDVWSFDIISWDVEGCYLSPFNVLQCYLESTL 1141
             EK+ E   +S+   + IKN Q   L+A D W      WD+  CYLSP++VLQCYLESTL
Sbjct: 1083 IEKHNEDGKISEIRTFSIKNFQVYRLLATDFWPCGNFLWDINRCYLSPWSVLQCYLESTL 1142

Query: 1142 QVGLVHEMIGNGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLARKELE 1201
            QVG+++E+IGNG EAET+L WGK+ SC QSL  F VAFSSALG +Y KKQ   LA KEL+
Sbjct: 1143 QVGILNELIGNGLEAETILSWGKAFSCSQSLFPFVVAFSSALGNLYHKKQCLDLAEKELQ 1202

Query: 1202 GAKEIL--TDSITSCLKCKLVLEVIVDQHLGDLFRSMYVNANGSISEGQLENAEELYKLA 1261
             AKEIL       SC+KCKL LEV +D+ LGD+ R        S ++G L +AE L+  A
Sbjct: 1203 NAKEILIANQRDFSCVKCKLKLEVTLDKQLGDISRKQIDRV--SQTDGFL-HAESLFSAA 1262

Query: 1262 LEKLNLSAWKNFISFPDEE----------------RYLSSLTIQAERPKARKDGRKCKKT 1321
            L K   SAWK+ I    EE                   S   +  + P   +  R+  + 
Sbjct: 1263 LGKFCCSAWKSCIRSHGEEIAEEIVIDRNGGEGLGHNSSKTKLSIKEPPGNRGSRRGGRA 1322

Query: 1322 TNASRPFQSDQCVNSQSNVRLTRSRCRTIQGQSTSNSNEVEVDLSVHLKSNIPDLSSASC 1381
                     DQ + S+   RLTRS   +++ Q  + SN  EV   V  K N+ D S  S 
Sbjct: 1323 NKTC--LSKDQDLISEPTSRLTRSMRHSLREQCQNRSNVPEV---VSKKPNLCDRSVGSR 1382

Query: 1382 QKQSYLQVNSCTPTFGCGASCKNGKVGCWQCLPTEIMEAGEMNNFIYLKWEFVRRRLLLR 1441
             ++  L  ++  P F     C   K    QCL  E+ E+G +NN + LKWE   R+L   
Sbjct: 1383 GERVLLDTSNALPGF-----CICYKEKRQQCLSEEVTESGSLNNLVSLKWELCHRKLASS 1442

Query: 1442 HLSGLGKCSEIRGQIHQTHETILKSMSILVSRNLFFQAHYAVEPMILLELVGKEVHRDMF 1501
             L  LGKC    G+IH  HE +L S+S+L      + +H       LLE +GKEV RD+F
Sbjct: 1443 ILVSLGKCLGDSGRIHLAHEALLHSISVLFKST--WSSHNQPSVSQLLEFIGKEVTRDVF 1502

Query: 1502 AVERASVLYNICCFSLKSYKYDDSKITCCPLSQVQSKTLVSWLMVALVLCCEVPVLFQKV 1561
            AV+RA +LYN+C  +L++Y    S+  CC L  +    LVSWLM+A VL  EVP+LFQKV
Sbjct: 1503 AVDRAIILYNLCWLNLRNYHCRKSRSICCDLFHIPFTKLVSWLMLAFVLSGEVPILFQKV 1562

Query: 1562 SKLLAVIHVISSSSELFSLPSSNKILTDSHWASYFHQASIGTHLNYQFFPYMTRR---SC 1621
            S+LLA ++++SSS+  F+  S    L+ SHW S+FHQAS+GTHL+Y F   ++++    C
Sbjct: 1563 SRLLASLYLLSSSNSEFTFESDGNELSASHWVSFFHQASLGTHLSYHFISNLSQKHKSQC 1622

Query: 1622 VEDLNFAKVLWNLLFFFVGFCRFLCCSSYNWIVAYVIFWIFLFVYFSLYLFSFFFLLIVD 1681
            + D                 C    CSS                                
Sbjct: 1623 LSDKE---------------CTEATCSS-------------------------------- 1682

Query: 1682 ACILHLKYGLIDGFNKVYFQGFDAGEQTLKLFRKGPFSSQDLEEHLRKFFDGLPCVTMVC 1741
             C++                      + L L R  P  +QDL +  ++FF  LP  T++C
Sbjct: 1683 -CMV---------------------PEDLDLPRLAPDRTQDLVQFAKEFFINLPSSTIIC 1742

Query: 1742 ISLIGGDLACLLQQILHFPSSVHAWILVSHLSSKRHPLVLLLPVETILKEGSEDYSNLQS 1801
            ISL+GG L  LLQ+++H  S V AW+L+S L+ +  P+  LLPV++I+++ S+D +NL S
Sbjct: 1743 ISLLGGALNQLLQELMHIRSPVCAWVLISRLNPESQPVATLLPVDSIVEDMSDDSANLSS 1802

Query: 1802 DDSCERNDLAKHWQCPWGSSVIDEIAPAFRIILEENYLSSSVFPLEDTKTNRMLWWKRRT 1861
             ++ +   L   W CPWG++V+DE+APAF+ ILEE++ SSS    EDT  +R LWWK+R 
Sbjct: 1803 TEATQVKSLKGPWLCPWGTTVVDEVAPAFKSILEESHSSSSTTE-EDTIESRGLWWKKRK 1862

Query: 1862 KLDDCLGKLLGTIEDSWLGPWKYMLLGDWSTREHFDSVLNTLVLNLKSKCKMDVNESLLK 1921
            KL+  LG  L  +E SWLGPW+ +LLG+WS  +  DS    LV +LKSKCKM+VNE LLK
Sbjct: 1863 KLNHRLGIFLRNLEASWLGPWRCLLLGEWSNYKLPDSAQKKLVNDLKSKCKMEVNEMLLK 1922

Query: 1922 VILEGSEEVL--EAFDSKLCSRKGCFIGKTRF-YDKERSNPFQNTLNGVDQLSALALKLI 1981
            VIL G  +    EA  ++L  R GC++G+  + Y+++         N  +    LALKLI
Sbjct: 1923 VILGGGTDNFKGEACVAQLSLRNGCYVGRGGYLYEEDSCKTPTAASNISESRHELALKLI 1982

Query: 1982 KDAKKEL---EGEENTCREPIILVLDYDVQMLPWENIPILQNQEVYRMPSVGCICATLDG 2041
             DA  +L   +G EN  REPIILVLD +VQMLPWENIPIL+ QEVYRMPSVGCI A L  
Sbjct: 1983 HDAASKLGQQDGHEN--REPIILVLDPEVQMLPWENIPILRKQEVYRMPSVGCISAVLKK 2042

Query: 2042 RSHQQEQDGGIIAAFPSIDPLDAFYLLNPSGDLNSTQIEFENWFKDQNLEGKAGYAPTSS 2101
            RS Q E     +A+FP IDPLD+FYLLNP GDL  TQ+ FE+WF+DQN EGKAG  P++ 
Sbjct: 2043 RSLQGEPAKSHVASFPLIDPLDSFYLLNPGGDLTDTQVTFESWFRDQNFEGKAGSEPSAI 2102

Query: 2102 ELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAASLLMGCSSGSLTLNGHYVPQGIP 2161
            EL E L++ DLF+YFGHGSGAQYIPR EI+KLD C+A+ LMGCSSGSL L G Y+PQG+P
Sbjct: 2103 ELTEALETHDLFLYFGHGSGAQYIPRREIEKLDNCSATFLMGCSSGSLWLKGCYIPQGVP 2162

Query: 2162 LSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRERSGALPSS--AQCDIVTKEFEAM 2221
            LSYL  GSP IVA LW+VTD+DIDRFGKA+LEAWL+ERS +      +QC+ +  +  AM
Sbjct: 2163 LSYLLGGSPAIVATLWDVTDRDIDRFGKALLEAWLQERSDSSSEGGCSQCESLANDLAAM 2171

Query: 2222 KISSKHANKKVASKSLPAACESESSSR--GHSAHSRMIGSFLCEAREACNLRYLIGASPV 2252
             +     ++K +S++ PA  + + S +   +  H R IGSF+  AR+ACNL+YLIGA+PV
Sbjct: 2223 TLKGTKRSRKPSSRNKPAQSDVDGSGKIECNHKHRRKIGSFIAAARDACNLQYLIGAAPV 2171

BLAST of Lcy11g005290 vs. ExPASy Swiss-Prot
Match: P18296 (Separin OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=cut1 PE=1 SV=3)

HSP 1 Score: 201.8 bits (512), Expect = 8.0e-50
Identity = 144/460 (31.30%), Postives = 205/460 (44.57%), Query Frame = 0

Query: 1796 FRIILEENYLSSS---VFPLEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYML 1855
            FRII + N ++ +       ED +T    WWK R  LD CL +LL  IE SWLG +K + 
Sbjct: 1429 FRIISKSNQMAQNGKHYTRREDKET----WWKERRHLDQCLQQLLENIEISWLGGFKGIF 1488

Query: 1856 LG---DWSTREHFDSVLNTLV---LNLKSKCKM-DVNESLLKVILEGSEEVLEAFDSKLC 1915
                 D S    F S    ++    N+  K  +  ++  +L++ +   +   E ++  L 
Sbjct: 1489 NPHKIDTSLFAKFSSQFQNIIAKNFNMDKKTPVPTLSPEILELFITLGKPGYEGYEQLLE 1548

Query: 1916 SRKGCFIGKTRFYDKERSNPFQNTLNGVDQLSALALKLIKDAKKELEGEENTCREPIILV 1975
                  +   +F    R   F       DQLS      +         EEN  R   +LV
Sbjct: 1549 DLIYFILDIFQF----RGLHFAYDEIDTDQLSMDLQDALNAYFNNYVSEEN--RSHTVLV 1608

Query: 1976 LDYDVQMLPWENIPILQNQEVYRMPSVGCICATLDGRSHQQEQDGGIIAAFPSIDPLDAF 2035
            LD  V   PWE++P L  Q V R+PS+  +   L        Q   +   +  +      
Sbjct: 1609 LDKSVHQFPWESLPCLNRQSVSRVPSLSILRDIL-------SQSFVVNGEYVEVRKEAGS 1668

Query: 2036 YLLNPSGDLNSTQIEFENWFKDQNLEGKAGYAPTSSELIEELKSRDLFIYFGHGSGAQYI 2095
            Y+LNPS DL  TQ  FE+   +   +G     P++ + I+ L   D F+YFGHG G QY 
Sbjct: 1669 YILNPSLDLKHTQEMFEHKLVEGGWKGLIASQPSNRDFIKMLSGNDFFLYFGHGGGEQYT 1728

Query: 2096 PRHEIQKLDACAASLLMGCSSGSLTLNGHYVPQGIPLSYLKAGSPVIVANLWEVTDKDID 2155
              +++  L  CA ++LMGCSSG+L   G + P G PL YL AG P +VANLW+VTDKDID
Sbjct: 1729 TSYDLATLKRCAVTILMGCSSGALYECGSFEPWGTPLDYLSAGCPTLVANLWDVTDKDID 1788

Query: 2156 RFGKAILEAWLRERSGALPSSAQCDIVTKEFEAMKISSKHANKKVASKSLPAACESESSS 2215
            RF   +LE+W     G   + A                                      
Sbjct: 1789 RFSLKMLESW-----GLFENKAP------------------------------------- 1826

Query: 2216 RGHSAHSRMIGSFLCEAREACNLRYLIGASPVCYGVPTSI 2246
                 +S  I + + E+R  C+LRYL GA+PV YG+P  I
Sbjct: 1849 ---FVNSTSICTAVSESRSCCHLRYLNGAAPVIYGIPAYI 1826

BLAST of Lcy11g005290 vs. ExPASy Swiss-Prot
Match: P33144 (Separin OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=bimB PE=4 SV=2)

HSP 1 Score: 199.9 bits (507), Expect = 3.1e-49
Identity = 154/481 (32.02%), Postives = 216/481 (44.91%), Query Frame = 0

Query: 1790 DEIAPAFRIILEENYLSSSVFPLEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWK 1849
            DE+    R+  E    S+    L+  +  +  WWK R  LD  +  LL  IE+ W G   
Sbjct: 1651 DEMKELIRLANE----SAHAAKLQVDRQMKKEWWKNREALDRRMENLLQNIENVWFG--- 1710

Query: 1850 YMLLGDWSTREHFDSVLNTLVLNLKSKCKM-DVNESLLKVILEGSEEVLEAFDSKLCSRK 1909
                        F  + + + L  KS  +     E++L+  L    +   A   KL    
Sbjct: 1711 -----------GFRGIFSPIPLCEKSLARFASAFENILENHLPSRRKGSRAQGPKLTLHP 1770

Query: 1910 G---CFIGKTRFYDKERSN--------------PFQNTLNGVDQLS-----ALALKLIKD 1969
                 F+G     D+E                  FQ   N  D++         L  ++ 
Sbjct: 1771 NVLELFVGVKGLDDQEDPEDTLMDLLYFVVDILQFQGERNAYDEVDFDMMVVETLDAVRA 1830

Query: 1970 AKKELEGEENTCREPIILVLDYDVQMLPWENIPILQNQEVYRMPSVGCI-CATLDGRSHQ 2029
              +  + +        +LVLD  + + PWE++P LQ   V R+PS+ C+    L  RS +
Sbjct: 1831 YHEAAKDQATQRPNNTVLVLDKSLHLFPWESLPCLQGLPVCRVPSLECLRDRVLHLRSGK 1890

Query: 2030 QEQDGGIIAAFPSIDPLDAFYLLNPSGDLNSTQIEFENWFKD-QNLEGKAGYA---PTSS 2089
            Q        +  SID  +  Y+LNP+GDL +TQ  FE   KD  +L+G  G     PT  
Sbjct: 1891 Q--------SALSIDRRNGTYILNPTGDLKTTQETFE---KDLSSLKGWTGMVNRQPTED 1950

Query: 2090 ELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAASLLMGCSSGSLTLNGHYVPQGIP 2149
            E  + L+S+ LF+YFGHGSGAQYI    +++LD CA + LMGCSSG+LT  G Y P G P
Sbjct: 1951 EFKDSLQSKSLFLYFGHGSGAQYIRGRTVKRLDRCAVAFLMGCSSGTLTEAGEYEPYGTP 2010

Query: 2150 LSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRERSGALPSSAQCDIVTKEFEAMKI 2209
            ++YL+AGSP +VA LW+VTDKDIDRF KA  E W                         I
Sbjct: 2011 MNYLQAGSPALVATLWDVTDKDIDRFAKATFEHW-----------------------GLI 2062

Query: 2210 SSKHANKKVASKSLPAACESESSSRGHSAHSRMIGSFLCEAREACNLRYLIGASPVCYGV 2243
             + H   +   ++  A   + S SRG                 AC L+YL GA+PV YGV
Sbjct: 2071 GNGHRGNEGIGEAGVALDAAVSQSRG-----------------ACVLKYLNGAAPVVYGV 2062

BLAST of Lcy11g005290 vs. ExPASy Swiss-Prot
Match: P60330 (Separin OS=Mus musculus OX=10090 GN=Espl1 PE=1 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 7.0e-46
Identity = 128/436 (29.36%), Postives = 203/436 (46.56%), Query Frame = 0

Query: 1817 TNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYMLL---GDWSTREHFDSVLNTLVLNL 1876
            T + +WW  R  LD  +  L+  +E+  LG W+ +LL    D S  +    +   L    
Sbjct: 1767 TEKRVWWTGRLALDQRMEALITALEEQVLGCWRGLLLPCSADPSLAQEASKLQELL---- 1826

Query: 1877 KSKCKMDVNES-LLKVILEGSEEVLEAFDSKLCSRKGCFIGKTRFYDKERSNPFQNTLNG 1936
              +C  +  +S LLKVIL G+  +L + D +  +   C     R                
Sbjct: 1827 -RECGWEYPDSTLLKVILSGA-RILTSQDVQALACGLCPAQPDR---------------- 1886

Query: 1937 VDQLSALALKLIKDAKKELEGEENTCREPIILVLDYDVQMLPWENIPILQNQEVYRMPSV 1996
                   A  L+ +A  +++ +E    + ++LVLD D+Q LPWE+ PILQ Q V R+PS 
Sbjct: 1887 -------AQVLLSEAVGQVQSQEAPRSQHLVLVLDKDLQKLPWESTPILQAQPVTRLPSF 1946

Query: 1997 GCICATLDGRSHQQEQDGGIIAAF-PSIDPLDAFYLLNPSGDLNSTQIEFENWFKDQ-NL 2056
              +       S+   ++ G  +     +DP + FY+LNP  +L+ST+  F   F  +   
Sbjct: 1947 RFLL------SYTVTKEAGASSVLSQGVDPQNTFYVLNPHSNLSSTEERFRASFSSETGW 2006

Query: 2057 EGKAGYAPTSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAASLLMGCSSGSLT 2116
            +G  G  P+  ++   L  RDL+IY GHG+GA+++    + +L   A +LL GCSS +L 
Sbjct: 2007 KGVIGEVPSLDQVQAALTERDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALA 2066

Query: 2117 LNGHYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRERSGALPSSAQCD 2176
            ++G+    GI L Y+ AG P+ + NLW+VTD+DIDR+ +A+L+ WL    GA        
Sbjct: 2067 VHGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGA-------- 2116

Query: 2177 IVTKEFEAMKISSKHANKKVASKSLPAACESESSSRGHSAHSRMIGSFLCEAREACNLRY 2236
                                                           +  +AR+A  L+Y
Sbjct: 2127 -------------------------------------------PFLYYASQARQAPRLKY 2116

Query: 2237 LIGASPVCYGVPTSIR 2247
            LIGA+PV YG+P S++
Sbjct: 2187 LIGAAPVAYGLPISLQ 2116

BLAST of Lcy11g005290 vs. ExPASy Swiss-Prot
Match: Q14674 (Separin OS=Homo sapiens OX=9606 GN=ESPL1 PE=1 SV=3)

HSP 1 Score: 183.7 bits (465), Expect = 2.3e-44
Identity = 126/436 (28.90%), Postives = 200/436 (45.87%), Query Frame = 0

Query: 1817 TNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYMLLGDWSTREHFDSVLNTLVLNLKSK 1876
            T++  WW  R  LD  +  L+ ++E S LG WK +LL   S+ E   +   + +  L   
Sbjct: 1771 TDKREWWTGRLALDHRMEVLIASLEKSVLGCWKGLLLP--SSEEPGPAQEASRLQELLQD 1830

Query: 1877 CKMDV-NESLLKVILEG----SEEVLEAFDSKLCSRKGCFIGKTRFYDKERSNPFQNTLN 1936
            C     + +LLK++L G    + + ++A    LC  +            ER         
Sbjct: 1831 CGWKYPDRTLLKIMLSGAGALTPQDIQALAYGLCPTQ-----------PER--------- 1890

Query: 1937 GVDQLSALALKLIKDAKKELEGEENTCREPIILVLDYDVQMLPWENIPILQNQEVYRMPS 1996
                    A +L+ +A   L+G        ++LVLD D+Q LPWE++P LQ   V R+PS
Sbjct: 1891 --------AQELLNEAVGRLQGLTVPSNSHLVLVLDKDLQKLPWESMPSLQALPVTRLPS 1950

Query: 1997 VGCICATLDGRSHQQEQDGGIIAAFPSIDPLDAFYLLNPSGDLNSTQIEFE-NWFKDQNL 2056
               + +    + +     G        +DP   FY+LNP  +L+ST+ +F  N+  +   
Sbjct: 1951 FRFLLSYSIIKEY-----GASPVLSQGVDPRSTFYVLNPHNNLSSTEEQFRANFSSEAGW 2010

Query: 2057 EGKAGYAPTSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAASLLMGCSSGSLT 2116
             G  G  P   ++ E L   DL+IY GHG+GA+++    + +L   A +LL GCSS +L 
Sbjct: 2011 RGVVGEVPRPEQVQEALTKHDLYIYAGHGAGARFLDGQAVLRLSCRAVALLFGCSSAALA 2070

Query: 2117 LNGHYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRERSGALPSSAQCD 2176
            + G+    GI L Y+ AG P+ + NLW+VTD+DIDR+ +A+L+ WL    GA        
Sbjct: 2071 VRGNLEGAGIVLKYIMAGCPLFLGNLWDVTDRDIDRYTEALLQGWLGAGPGA-------- 2120

Query: 2177 IVTKEFEAMKISSKHANKKVASKSLPAACESESSSRGHSAHSRMIGSFLCEAREACNLRY 2236
                                                        +  ++ +AR+A  L+Y
Sbjct: 2131 -------------------------------------------PLLYYVNQARQAPRLKY 2120

Query: 2237 LIGASPVCYGVPTSIR 2247
            LIGA+P+ YG+P S+R
Sbjct: 2191 LIGAAPIAYGLPVSLR 2120

BLAST of Lcy11g005290 vs. ExPASy TrEMBL
Match: A0A6J1FX68 (Separase OS=Cucurbita moschata OX=3662 GN=LOC111447831 PE=4 SV=1)

HSP 1 Score: 3745.7 bits (9712), Expect = 0.0e+00
Identity = 1884/2253 (83.62%), Postives = 2003/2253 (88.90%), Query Frame = 0

Query: 1    MASPSESSLISILETADSKGISSLVSDFLQPFSDVKNPKKCKKSAKSTDDSSTIRSLAKK 60
            MASPSE+S+ISILETADSKGI S VSDFL+PFSD+KNPKK KKSAK +DDSS IRSLAKK
Sbjct: 1    MASPSEASIISILETADSKGIFSTVSDFLRPFSDIKNPKKSKKSAKPSDDSSAIRSLAKK 60

Query: 61   FLAFLNRALSILPKRLSEPSKLGNDLELALEFFEVYKLCLGCLESLTSQLSCKPYTVDVQ 120
            FLAFLNRALSILPKRLSEPSKLGNDLELALEFFE+Y+LCL CLESLTSQLSCKPYTVDVQ
Sbjct: 61   FLAFLNRALSILPKRLSEPSKLGNDLELALEFFEIYRLCLACLESLTSQLSCKPYTVDVQ 120

Query: 121  RVRMVHCMEDWGLFKDAEAEGFGILERLREIDRKSKAGKLDCRVIHDGDKGGGDKDFCLL 180
            RVRMVHCMEDWGLFKDAEAEGF ILERLR IDR S     DCRVIHD DKGGGDK+FCLL
Sbjct: 121  RVRMVHCMEDWGLFKDAEAEGFRILERLRVIDRTS-----DCRVIHDRDKGGGDKEFCLL 180

Query: 181  FAEVVVTIVKCTASGRSKESGDYRRVLGLVEEVRPWFRLLDAKVSEKTQRALVTYLGKCT 240
            FAEVVVT+VKC ASGRSKESGDY R+LGLVEEVRPWFRLLDAKVSEKTQRAL TYLGKCT
Sbjct: 181  FAEVVVTLVKCAASGRSKESGDYGRLLGLVEEVRPWFRLLDAKVSEKTQRALATYLGKCT 240

Query: 241  IFLVEEVVCFGESLVSLFCRTTFAEYAKSSMRDQIYKLARRICSTLFSLQQEQHTSMLIM 300
            IFLVEE+  FGESLVSLFCR TFAEYAKSS++DQIYKLARRICSTLFSLQQEQH+SMLIM
Sbjct: 241  IFLVEELDYFGESLVSLFCRITFAEYAKSSLKDQIYKLARRICSTLFSLQQEQHSSMLIM 300

Query: 301  KILACVLKSITLEWKDETECTVIEFLQLICYCANKCQSESPECCCTFARHLEEMAADFHQ 360
             IL CV+KS+TLEWKDETE  V+EFLQLICY ANKC S SPECCC FA+HL+EMA +FHQ
Sbjct: 301  HILICVVKSLTLEWKDETEWEVVEFLQLICYSANKCLSTSPECCCAFAKHLQEMAGEFHQ 360

Query: 361  ATTPFGMNLRLYAAGLKIFSKVPRGETHGSAFSILVDDEDTMQSLVNLNGFLGSYFCIGC 420
            AT P GMNLRLYAAGLKI SK+PRGET GSAFS L+DDEDT+QSLVNLNGFLGSYFCIGC
Sbjct: 361  ATPPLGMNLRLYAAGLKIVSKLPRGETCGSAFSTLLDDEDTIQSLVNLNGFLGSYFCIGC 420

Query: 421  RKGNESCSNEQKDFVDQPCSHGKSNHECDVP-LLSWREAYLFSYLDAIKFLCQPLAESVN 480
            R+GNESCS EQKDFV QPC H  SNHE  VP LLS REAYL SYLDAIKF CQPLAESVN
Sbjct: 421  REGNESCSIEQKDFVGQPCLHRNSNHENGVPSLLSLREAYLSSYLDAIKFFCQPLAESVN 480

Query: 481  SERKEILAEDKAASVLYSIQNTLYQSCDVFLFCQRRAYDAKSDGCDENVNMLLSVVMAAF 540
             ERKEILAEDKAA VLY+IQNTL+Q CDVFLFCQRRAYDAKSDGCDENVNMLLS+V+AAF
Sbjct: 481  VERKEILAEDKAAPVLYNIQNTLHQFCDVFLFCQRRAYDAKSDGCDENVNMLLSIVVAAF 540

Query: 541  TLSIRTRLEMKRSTDLIKAVISSKWVQPFALKHLFASLNNIGIILYRNKQIREASKALKL 600
            TLS RTRL+MKRST+LIK VI+SKWVQPFALK LFASLNNIGIIL+RNKQI EASKALKL
Sbjct: 541  TLSFRTRLDMKRSTNLIKDVIASKWVQPFALKRLFASLNNIGIILFRNKQIGEASKALKL 600

Query: 601  CCRASWTCVRHFCVMFDDKAKPSDNEFSENAVLSFVNEVIMRSAFLVDILYQRGMHKVEK 660
            CCRASWTCV+ FC +FD+K++PSDNEFSEN+VL  VNEVIMRSAFLVDILYQ  M KVEK
Sbjct: 601  CCRASWTCVKQFCEIFDNKSRPSDNEFSENSVLIIVNEVIMRSAFLVDILYQCDMCKVEK 660

Query: 661  AVTEILKNWSMAEKLFNGLPVPVQLVKQWVKMQCKHHKNADPRKTTPTLNCLLQSSGMLS 720
            A+TEIL NWS A KLF+ LPVPVQLVKQWVKMQCK+HKNADP+K+TPTLNCLLQSS  +S
Sbjct: 661  AMTEILTNWSAAAKLFDELPVPVQLVKQWVKMQCKYHKNADPKKSTPTLNCLLQSSETVS 720

Query: 721  KAQIGALLEQELVEYAEMSGLYPEFCQSLQMKILSTLLRDVYVTPDHRLEKARVLLKKAR 780
            KA+IG LLEQELVEY EMSGLYPEFCQSLQMKILS LL D Y+TPDHRLEKARVL+KKAR
Sbjct: 721  KARIGVLLEQELVEYEEMSGLYPEFCQSLQMKILSILLTDTYITPDHRLEKARVLMKKAR 780

Query: 781  VLRFCETICLDNCIQCLSEAISTINETSGGTCSSGVLHSHQLAVAYCLRALCTHETVPNS 840
            VLR CET+CLD CIQCLSEAIST+NE S G+ S GVLHSHQLAVAYCLRA CTHETVPNS
Sbjct: 781  VLRLCETVCLDGCIQCLSEAISTMNEISSGSRSFGVLHSHQLAVAYCLRAFCTHETVPNS 840

Query: 841  KQVLQDIDSALSVWLEISSLDSLPDDQQLILSEYMLLLLCNTFDLLSIKGCMDYYHNIYS 900
            KQV QDIDSALS+WLEISSL++LPDDQ+LILSEYMLLLLCN FDLLSIKGC+DY+ NIYS
Sbjct: 841  KQVQQDIDSALSIWLEISSLNTLPDDQRLILSEYMLLLLCNAFDLLSIKGCVDYHDNIYS 900

Query: 901  LMIRLFKWKNVQLEKLLAVLWEGRRMSHAICIAPANEIVITQLSEHLGELPKSFNFWTNC 960
            LMIRLFKWKNV LEKL+A LWE RRMSHA+CIAPANEIVI QLSEHLG+LPKS +FWTNC
Sbjct: 901  LMIRLFKWKNVPLEKLMATLWESRRMSHAMCIAPANEIVIAQLSEHLGDLPKSNDFWTNC 960

Query: 961  LKTLPGMLVGFRQNFSFLCRNNGQSCCEHEKSVQLDVTVDEVKEAASKLISHTPACKSSI 1020
            LKTLPGMLVGF+QNFSFLC N  Q+ CEHEKS ++ VTVDEVKEAA KLISH PACKSSI
Sbjct: 961  LKTLPGMLVGFQQNFSFLCSNYTQTSCEHEKSSRMHVTVDEVKEAALKLISHIPACKSSI 1020

Query: 1021 FLAGYLYYDLCERLISEGCLTEALSCAKEAHRLRSKLFQEKFTYSIEQHPEKYCEISHVS 1080
            F AGYLYYDLCERLISEGCLTEAL CAKEAHRLRSKLFQEKFTYS+EQHPEKYCEI+H S
Sbjct: 1021 FSAGYLYYDLCERLISEGCLTEALLCAKEAHRLRSKLFQEKFTYSVEQHPEKYCEITHAS 1080

Query: 1081 QKPAYGIKNLQKNGLVARDVWSFDIISWDVEGCYLSPFNVLQCYLESTLQVGLVHEMIGN 1140
            QKP YGIKNL+K+G V+R++WSFD ISWDVEGCYLSPFNVLQCYLESTLQVGLVHE+IGN
Sbjct: 1081 QKPPYGIKNLRKSGSVSRNIWSFDKISWDVEGCYLSPFNVLQCYLESTLQVGLVHEIIGN 1140

Query: 1141 GSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLARKELEGAKEILTDSIT 1200
            GSEAETLLQWGKSISCL+SLPLFEVAFSSALGKVYRKKQLWSLA+KELEGAK+ILTD+IT
Sbjct: 1141 GSEAETLLQWGKSISCLRSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDNIT 1200

Query: 1201 SCLKCKLVLEVIVDQHLGDLFRSMYVNANGSISEGQLENAEELYKLALEKLNLSAWKNFI 1260
            SCLKC+LVLEVIVDQ+LGDLFRSMYVN NG+ SE  L NAEELYKLALEKLNLS WKN I
Sbjct: 1201 SCLKCRLVLEVIVDQYLGDLFRSMYVNGNGNTSEELLINAEELYKLALEKLNLSGWKNSI 1260

Query: 1261 SFPDEERYLSSLTIQAERPKARKDGRKCKKTTNASRPFQSDQCVNSQSNVRLTRSRCRTI 1320
            S PDE+ +LSSLTI  ER KARKDGRK KKTTNA R  + DQCVN Q+NVRLTRSRCR+I
Sbjct: 1261 SCPDEDSHLSSLTIPVERAKARKDGRKSKKTTNAPRSLKMDQCVNPQTNVRLTRSRCRSI 1320

Query: 1321 QGQSTSNSNEVEVDLSVHLKSNIPDLSSASCQKQSYLQVNSCTPTFGCGASCKNGKVGCW 1380
            QGQST+ SNEVEVDL+VHLKSN+PDL  AS QKQ +LQVN CTPT GCGASCKNGKVGCW
Sbjct: 1321 QGQSTNISNEVEVDLTVHLKSNVPDLFGASGQKQMHLQVNCCTPTLGCGASCKNGKVGCW 1380

Query: 1381 QCLPTEIMEAGEMNNFIYLKWEFVRRRLLLRHLSGLGKCSEIRGQIHQTHETILKSMSIL 1440
            QCL  EIMEAG++NNFIYLKWEFVRRRLLLR LSGLGKCS IRGQIHQTHETILKSMSIL
Sbjct: 1381 QCLQMEIMEAGQINNFIYLKWEFVRRRLLLRQLSGLGKCSGIRGQIHQTHETILKSMSIL 1440

Query: 1441 VSRNLFFQAHYAVEPMILLELVGKEVHRDMFAVERASVLYNICCFSLKSYKYDDSKITCC 1500
            VSRN+F QAH  VEP++LL+LVGKEV  DMFAVERASVLYNIC F+LKSYKYDD+KI CC
Sbjct: 1441 VSRNIFSQAHGVVEPVVLLDLVGKEVPGDMFAVERASVLYNICWFALKSYKYDDTKIICC 1500

Query: 1501 PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSSSELFSLPSSNKILTDS 1560
            PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSS EL SLPSSNKILTDS
Sbjct: 1501 PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSSIELLSLPSSNKILTDS 1560

Query: 1561 HWASYFHQASIGTHLNYQFFPYMTRRSCVEDLNFAKVLWNLLFFFVGFCRFLCCSSYNWI 1620
            HWASYFHQASIGTHLN+QFFPY T RSC++DLNFAK                        
Sbjct: 1561 HWASYFHQASIGTHLNHQFFPYTTGRSCIQDLNFAK------------------------ 1620

Query: 1621 VAYVIFWIFLFVYFSLYLFSFFFLLIVDACILHLKYGLIDGFNKVYFQGFDAGEQTLKLF 1680
                                                            GFD GEQT+KLF
Sbjct: 1621 ------------------------------------------------GFDTGEQTVKLF 1680

Query: 1681 RKGPFSSQDLEEHLRKFFDGLPCVTMVCISLIGGDLACLLQQILHFPSSVHAWILVSHLS 1740
            R+G FSSQDLEE++RKFFDGLPCV MVCISLIGGDLACLLQQILHFPSSVHAWILVSHL+
Sbjct: 1681 RRGSFSSQDLEEYVRKFFDGLPCVAMVCISLIGGDLACLLQQILHFPSSVHAWILVSHLN 1740

Query: 1741 SKRHPLVLLLPVETILKEGSEDYSNLQSDDSCERNDLAKHWQCPWGSSVIDEIAPAFRII 1800
            SKR PLVLLLPVETILKE SEDYSN QSDD CERND  KHWQCPWGSSVIDEIAPAFR I
Sbjct: 1741 SKRQPLVLLLPVETILKEDSEDYSNPQSDDICERNDSTKHWQCPWGSSVIDEIAPAFRTI 1800

Query: 1801 LEENYLSSSVFPLEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYMLLGDWSTR 1860
            LE+NYLSSSVFP EDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKY+LLGDWS R
Sbjct: 1801 LEDNYLSSSVFPSEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYILLGDWSNR 1860

Query: 1861 EHFDSVLNTLVLNLKSKCKMDVNESLLKVILEGSEEVLEAFDSKLCSRKGCFIGKTRFYD 1920
            +H DSVLNTLVLNLKSKCKMDVNE LLK+ILEGSE VL  FDSKL SRKGCF+G+ RF+D
Sbjct: 1861 KHVDSVLNTLVLNLKSKCKMDVNEGLLKIILEGSENVLVGFDSKLYSRKGCFVGRARFHD 1920

Query: 1921 KERSNPFQNTLNGVDQLSALALKLIKDAKKELEGEENTCREPIILVLDYDVQMLPWENIP 1980
            KERSNPFQN LNGVD+LS LALKLI+DAKKELEGE+ T REPIILVLDYDVQMLPWENIP
Sbjct: 1921 KERSNPFQNALNGVDKLSTLALKLIQDAKKELEGEDGTSREPIILVLDYDVQMLPWENIP 1980

Query: 1981 ILQNQEVYRMPSVGCICATLDGRSHQQEQDGGIIAAFPSIDPLDAFYLLNPSGDLNSTQI 2040
            IL+NQEVYRMPSVG IC TLDGR HQQEQD GIIA FPSIDPLDAFYLLNPSGDL+STQI
Sbjct: 1981 ILKNQEVYRMPSVGSICVTLDGRLHQQEQDSGIIATFPSIDPLDAFYLLNPSGDLSSTQI 2040

Query: 2041 EFENWFKDQNLEGKAGYAPTSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS 2100
            EFENWFKDQNLEGKAGYAP SSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS
Sbjct: 2041 EFENWFKDQNLEGKAGYAPKSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS 2100

Query: 2101 LLMGCSSGSLTLNGHYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER 2160
            LLMGCSSGSLTLNG+YVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER
Sbjct: 2101 LLMGCSSGSLTLNGYYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER 2160

Query: 2161 SGALPSSAQCDIVTKEFEAMKISSKHANKKVASKSLPAACESESSSRGHSAHSRMIGSFL 2220
            S ALPSS QCDIVTKE EAMKISSK ANKKVASKSLP  CES SSS+ HS HSRMIGSFL
Sbjct: 2161 SCALPSSVQCDIVTKELEAMKISSKLANKKVASKSLPTTCES-SSSKDHSVHSRMIGSFL 2175

Query: 2221 CEAREACNLRYLIGASPVCYGVPTSIRKKKDLT 2253
            CEAREACNLRYLIGASPVCYGVPTSIRKKK+LT
Sbjct: 2221 CEAREACNLRYLIGASPVCYGVPTSIRKKKELT 2175

BLAST of Lcy11g005290 vs. ExPASy TrEMBL
Match: A0A6J1FW16 (Separase OS=Cucurbita moschata OX=3662 GN=LOC111447831 PE=4 SV=1)

HSP 1 Score: 3740.3 bits (9698), Expect = 0.0e+00
Identity = 1884/2253 (83.62%), Postives = 2002/2253 (88.86%), Query Frame = 0

Query: 1    MASPSESSLISILETADSKGISSLVSDFLQPFSDVKNPKKCKKSAKSTDDSSTIRSLAKK 60
            MASPSE+S+ISILETADSKGI S VSDFL+PFSD+KNPKK KKSAK +DDSS IRSLAKK
Sbjct: 1    MASPSEASIISILETADSKGIFSTVSDFLRPFSDIKNPKKSKKSAKPSDDSSAIRSLAKK 60

Query: 61   FLAFLNRALSILPKRLSEPSKLGNDLELALEFFEVYKLCLGCLESLTSQLSCKPYTVDVQ 120
            FLAFLNRALSILPKRLSEPSKLGNDLELALEFFE+Y+LCL CLESLTSQLSCKPYTVDVQ
Sbjct: 61   FLAFLNRALSILPKRLSEPSKLGNDLELALEFFEIYRLCLACLESLTSQLSCKPYTVDVQ 120

Query: 121  RVRMVHCMEDWGLFKDAEAEGFGILERLREIDRKSKAGKLDCRVIHDGDKGGGDKDFCLL 180
            RVRMVHCMEDWGLFKDAEAEGF ILERLR IDR S     DCRVIHD DKGGGDK+FCLL
Sbjct: 121  RVRMVHCMEDWGLFKDAEAEGFRILERLRVIDRTS-----DCRVIHDRDKGGGDKEFCLL 180

Query: 181  FAEVVVTIVKCTASGRSKESGDYRRVLGLVEEVRPWFRLLDAKVSEKTQRALVTYLGKCT 240
            FAEVVVT+VKC ASGRSKESGDY R+LGLVEEVRPWFRLLDAKVSEKTQRAL TYLGKCT
Sbjct: 181  FAEVVVTLVKCAASGRSKESGDYGRLLGLVEEVRPWFRLLDAKVSEKTQRALATYLGKCT 240

Query: 241  IFLVEEVVCFGESLVSLFCRTTFAEYAKSSMRDQIYKLARRICSTLFSLQQEQHTSMLIM 300
            IFLVEE+  FGESLVSLFCR TFAEYAKSS++DQIYKLARRICSTLFSLQQEQH+SMLIM
Sbjct: 241  IFLVEELDYFGESLVSLFCRITFAEYAKSSLKDQIYKLARRICSTLFSLQQEQHSSMLIM 300

Query: 301  KILACVLKSITLEWKDETECTVIEFLQLICYCANKCQSESPECCCTFARHLEEMAADFHQ 360
             IL CV+KS+TLEWKDETE  V+EFLQLICY ANKC S SPECCC FA+HL+EMA +FHQ
Sbjct: 301  HILICVVKSLTLEWKDETEWEVVEFLQLICYSANKCLSTSPECCCAFAKHLQEMAGEFHQ 360

Query: 361  ATTPFGMNLRLYAAGLKIFSKVPRGETHGSAFSILVDDEDTMQSLVNLNGFLGSYFCIGC 420
            AT P GMNLRLYAAGLKI SK+PRGET GSAFS L+DDEDT+QSLVNLNGFLGSYFCIGC
Sbjct: 361  ATPPLGMNLRLYAAGLKIVSKLPRGETCGSAFSTLLDDEDTIQSLVNLNGFLGSYFCIGC 420

Query: 421  RKGNESCSNEQKDFVDQPCSHGKSNHECDVP-LLSWREAYLFSYLDAIKFLCQPLAESVN 480
            R+GNESCS EQKDFV QPC H  SNHE  VP LLS REAYL SYLDAIKF CQPLAESVN
Sbjct: 421  REGNESCSIEQKDFVGQPCLHRNSNHENGVPSLLSLREAYLSSYLDAIKFFCQPLAESVN 480

Query: 481  SERKEILAEDKAASVLYSIQNTLYQSCDVFLFCQRRAYDAKSDGCDENVNMLLSVVMAAF 540
             ERKEILAEDKAA VLY+IQNTL+Q CDVFLFCQRRAYDAKSDGCDENVNMLLS+V+AAF
Sbjct: 481  VERKEILAEDKAAPVLYNIQNTLHQFCDVFLFCQRRAYDAKSDGCDENVNMLLSIVVAAF 540

Query: 541  TLSIRTRLEMKRSTDLIKAVISSKWVQPFALKHLFASLNNIGIILYRNKQIREASKALKL 600
            TLS RTRL+MKRST+LIK VI+SKWVQPFALK LFASLNNIGIIL+RNKQI EASKALKL
Sbjct: 541  TLSFRTRLDMKRSTNLIKDVIASKWVQPFALKRLFASLNNIGIILFRNKQIGEASKALKL 600

Query: 601  CCRASWTCVRHFCVMFDDKAKPSDNEFSENAVLSFVNEVIMRSAFLVDILYQRGMHKVEK 660
            CCRASWTCV+ FC +FD+K++PSDNEFSEN+VL  VNEVIMRSAFLVDILYQ  M KVEK
Sbjct: 601  CCRASWTCVKQFCEIFDNKSRPSDNEFSENSVLIIVNEVIMRSAFLVDILYQCDMCKVEK 660

Query: 661  AVTEILKNWSMAEKLFNGLPVPVQLVKQWVKMQCKHHKNADPRKTTPTLNCLLQSSGMLS 720
            A+TEIL NWS A KLF+ LPVPVQLVKQWVKMQCK+HKNADP+K+TPTLNCLLQSS  +S
Sbjct: 661  AMTEILTNWSAAAKLFDELPVPVQLVKQWVKMQCKYHKNADPKKSTPTLNCLLQSSETVS 720

Query: 721  KAQIGALLEQELVEYAEMSGLYPEFCQSLQMKILSTLLRDVYVTPDHRLEKARVLLKKAR 780
            KA+IG LLEQELVEY EMSGLYPEFCQSLQMKILS LL D Y+TPDHRLEKARVL+KKAR
Sbjct: 721  KARIGVLLEQELVEYEEMSGLYPEFCQSLQMKILSILLTDTYITPDHRLEKARVLMKKAR 780

Query: 781  VLRFCETICLDNCIQCLSEAISTINETSGGTCSSGVLHSHQLAVAYCLRALCTHETVPNS 840
            VLR CET+CLD CIQCLSEAIST+NE S G+ S GVLHSHQLAVAYCLRA CTHETVPNS
Sbjct: 781  VLRLCETVCLDGCIQCLSEAISTMNEISSGSRSFGVLHSHQLAVAYCLRAFCTHETVPNS 840

Query: 841  KQVLQDIDSALSVWLEISSLDSLPDDQQLILSEYMLLLLCNTFDLLSIKGCMDYYHNIYS 900
            KQV QDIDSALS+WLEISSL++LPDDQ+LILSEYMLLLLCN FDLLSIKGC+DY+ NIYS
Sbjct: 841  KQVQQDIDSALSIWLEISSLNTLPDDQRLILSEYMLLLLCNAFDLLSIKGCVDYHDNIYS 900

Query: 901  LMIRLFKWKNVQLEKLLAVLWEGRRMSHAICIAPANEIVITQLSEHLGELPKSFNFWTNC 960
            LMIRLFKWKNV LEKL+A LWE RRMSHA+CIAPANEIVI QLSEHLG+LPKS +FWTNC
Sbjct: 901  LMIRLFKWKNVPLEKLMATLWESRRMSHAMCIAPANEIVIAQLSEHLGDLPKSNDFWTNC 960

Query: 961  LKTLPGMLVGFRQNFSFLCRNNGQSCCEHEKSVQLDVTVDEVKEAASKLISHTPACKSSI 1020
            LKTLPGMLVGF+QNFSFLC N  Q+ CEHEKS ++ VTVDEVKEAA KLISH PACKSSI
Sbjct: 961  LKTLPGMLVGFQQNFSFLCSNYTQTSCEHEKSSRMHVTVDEVKEAALKLISHIPACKSSI 1020

Query: 1021 FLAGYLYYDLCERLISEGCLTEALSCAKEAHRLRSKLFQEKFTYSIEQHPEKYCEISHVS 1080
            F AGYLYYDLCERLISEGCLTEAL CAKEAHRLRSKLFQEKFTYS+EQHPEKYCEI+H S
Sbjct: 1021 FSAGYLYYDLCERLISEGCLTEALLCAKEAHRLRSKLFQEKFTYSVEQHPEKYCEITHAS 1080

Query: 1081 QKPAYGIKNLQKNGLVARDVWSFDIISWDVEGCYLSPFNVLQCYLESTLQVGLVHEMIGN 1140
            QKP YGIKNL+K+G V+R++WSFD ISWDVEGCYLSPFNVLQCYLESTLQVGLVHE+IGN
Sbjct: 1081 QKPPYGIKNLRKSGSVSRNIWSFDKISWDVEGCYLSPFNVLQCYLESTLQVGLVHEIIGN 1140

Query: 1141 GSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLARKELEGAKEILTDSIT 1200
            GSEAETLLQWGKSISCL+SLPLFEVAFSSALGKVYRKKQLWSLA+KELEGAK+ILTD+IT
Sbjct: 1141 GSEAETLLQWGKSISCLRSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDNIT 1200

Query: 1201 SCLKCKLVLEVIVDQHLGDLFRSMYVNANGSISEGQLENAEELYKLALEKLNLSAWKNFI 1260
            SCLKC+LVLEVIVDQ+LGDLFRSMYVN NG+ SE  L NAEELYKLALEKLNLS WKN I
Sbjct: 1201 SCLKCRLVLEVIVDQYLGDLFRSMYVNGNGNTSEELLINAEELYKLALEKLNLSGWKNSI 1260

Query: 1261 SFPDEERYLSSLTIQAERPKARKDGRKCKKTTNASRPFQSDQCVNSQSNVRLTRSRCRTI 1320
            S PDE+ +LSSLTI  ER KARKDGRK KKTTNA R  + DQCVN Q+NVRLTRSRCR+I
Sbjct: 1261 SCPDEDSHLSSLTIPVERAKARKDGRKSKKTTNAPRSLKMDQCVNPQTNVRLTRSRCRSI 1320

Query: 1321 QGQSTSNSNEVEVDLSVHLKSNIPDLSSASCQKQSYLQVNSCTPTFGCGASCKNGKVGCW 1380
            QGQST+ SNEVEVDL+VHLKSN+PDL  AS QKQ +LQVN CTPT GCGASCKNGKVGCW
Sbjct: 1321 QGQSTNISNEVEVDLTVHLKSNVPDLFGASGQKQMHLQVNCCTPTLGCGASCKNGKVGCW 1380

Query: 1381 QCLPTEIMEAGEMNNFIYLKWEFVRRRLLLRHLSGLGKCSEIRGQIHQTHETILKSMSIL 1440
            QCL  EIMEAG++NNFIYLKWEFVRRRLLLR LSGLGKCS IRGQIHQTHETILKSMSIL
Sbjct: 1381 QCLQMEIMEAGQINNFIYLKWEFVRRRLLLRQLSGLGKCSGIRGQIHQTHETILKSMSIL 1440

Query: 1441 VSRNLFFQAHYAVEPMILLELVGKEVHRDMFAVERASVLYNICCFSLKSYKYDDSKITCC 1500
            VSRN+F QAH  VEP++LL+LVGKEV  DMFAVERASVLYNIC F+LKSYKYDD+KI CC
Sbjct: 1441 VSRNIFSQAHGVVEPVVLLDLVGKEVPGDMFAVERASVLYNICWFALKSYKYDDTKIICC 1500

Query: 1501 PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSSSELFSLPSSNKILTDS 1560
            PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSS EL SLPSSNKILTDS
Sbjct: 1501 PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSSIELLSLPSSNKILTDS 1560

Query: 1561 HWASYFHQASIGTHLNYQFFPYMTRRSCVEDLNFAKVLWNLLFFFVGFCRFLCCSSYNWI 1620
            HWASYFHQASIGTHLN+QFFPY T RSC++DLNFAK                        
Sbjct: 1561 HWASYFHQASIGTHLNHQFFPYTTGRSCIQDLNFAK------------------------ 1620

Query: 1621 VAYVIFWIFLFVYFSLYLFSFFFLLIVDACILHLKYGLIDGFNKVYFQGFDAGEQTLKLF 1680
                                                            GFD GEQT+KLF
Sbjct: 1621 ------------------------------------------------GFDTGEQTVKLF 1680

Query: 1681 RKGPFSSQDLEEHLRKFFDGLPCVTMVCISLIGGDLACLLQQILHFPSSVHAWILVSHLS 1740
            R G FSSQDLEE++RKFFDGLPCV MVCISLIGGDLACLLQQILHFPSSVHAWILVSHL+
Sbjct: 1681 R-GSFSSQDLEEYVRKFFDGLPCVAMVCISLIGGDLACLLQQILHFPSSVHAWILVSHLN 1740

Query: 1741 SKRHPLVLLLPVETILKEGSEDYSNLQSDDSCERNDLAKHWQCPWGSSVIDEIAPAFRII 1800
            SKR PLVLLLPVETILKE SEDYSN QSDD CERND  KHWQCPWGSSVIDEIAPAFR I
Sbjct: 1741 SKRQPLVLLLPVETILKEDSEDYSNPQSDDICERNDSTKHWQCPWGSSVIDEIAPAFRTI 1800

Query: 1801 LEENYLSSSVFPLEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYMLLGDWSTR 1860
            LE+NYLSSSVFP EDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKY+LLGDWS R
Sbjct: 1801 LEDNYLSSSVFPSEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYILLGDWSNR 1860

Query: 1861 EHFDSVLNTLVLNLKSKCKMDVNESLLKVILEGSEEVLEAFDSKLCSRKGCFIGKTRFYD 1920
            +H DSVLNTLVLNLKSKCKMDVNE LLK+ILEGSE VL  FDSKL SRKGCF+G+ RF+D
Sbjct: 1861 KHVDSVLNTLVLNLKSKCKMDVNEGLLKIILEGSENVLVGFDSKLYSRKGCFVGRARFHD 1920

Query: 1921 KERSNPFQNTLNGVDQLSALALKLIKDAKKELEGEENTCREPIILVLDYDVQMLPWENIP 1980
            KERSNPFQN LNGVD+LS LALKLI+DAKKELEGE+ T REPIILVLDYDVQMLPWENIP
Sbjct: 1921 KERSNPFQNALNGVDKLSTLALKLIQDAKKELEGEDGTSREPIILVLDYDVQMLPWENIP 1980

Query: 1981 ILQNQEVYRMPSVGCICATLDGRSHQQEQDGGIIAAFPSIDPLDAFYLLNPSGDLNSTQI 2040
            IL+NQEVYRMPSVG IC TLDGR HQQEQD GIIA FPSIDPLDAFYLLNPSGDL+STQI
Sbjct: 1981 ILKNQEVYRMPSVGSICVTLDGRLHQQEQDSGIIATFPSIDPLDAFYLLNPSGDLSSTQI 2040

Query: 2041 EFENWFKDQNLEGKAGYAPTSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS 2100
            EFENWFKDQNLEGKAGYAP SSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS
Sbjct: 2041 EFENWFKDQNLEGKAGYAPKSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS 2100

Query: 2101 LLMGCSSGSLTLNGHYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER 2160
            LLMGCSSGSLTLNG+YVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER
Sbjct: 2101 LLMGCSSGSLTLNGYYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER 2160

Query: 2161 SGALPSSAQCDIVTKEFEAMKISSKHANKKVASKSLPAACESESSSRGHSAHSRMIGSFL 2220
            S ALPSS QCDIVTKE EAMKISSK ANKKVASKSLP  CES SSS+ HS HSRMIGSFL
Sbjct: 2161 SCALPSSVQCDIVTKELEAMKISSKLANKKVASKSLPTTCES-SSSKDHSVHSRMIGSFL 2174

Query: 2221 CEAREACNLRYLIGASPVCYGVPTSIRKKKDLT 2253
            CEAREACNLRYLIGASPVCYGVPTSIRKKK+LT
Sbjct: 2221 CEAREACNLRYLIGASPVCYGVPTSIRKKKELT 2174

BLAST of Lcy11g005290 vs. ExPASy TrEMBL
Match: A0A6J1FRN9 (Separase OS=Cucurbita moschata OX=3662 GN=LOC111447831 PE=4 SV=1)

HSP 1 Score: 3739.9 bits (9697), Expect = 0.0e+00
Identity = 1883/2253 (83.58%), Postives = 2003/2253 (88.90%), Query Frame = 0

Query: 1    MASPSESSLISILETADSKGISSLVSDFLQPFSDVKNPKKCKKSAKSTDDSSTIRSLAKK 60
            MASPSE+S+ISILETADSKGI S VSDFL+PFSD+KNPKK KKSAK +DDSS IRSLAKK
Sbjct: 1    MASPSEASIISILETADSKGIFSTVSDFLRPFSDIKNPKKSKKSAKPSDDSSAIRSLAKK 60

Query: 61   FLAFLNRALSILPKRLSEPSKLGNDLELALEFFEVYKLCLGCLESLTSQLSCKPYTVDVQ 120
            FLAFLNRALSILPKRLSEPSKLGNDLELALEFFE+Y+LCL CLESLTSQLSCKPYTVDVQ
Sbjct: 61   FLAFLNRALSILPKRLSEPSKLGNDLELALEFFEIYRLCLACLESLTSQLSCKPYTVDVQ 120

Query: 121  RVRMVHCMEDWGLFKDAEAEGFGILERLREIDRKSKAGKLDCRVIHDGDKGGGDKDFCLL 180
            RVRMVHCMEDWGLFKDAEAEGF ILERLR IDR S     DCRVIHD DKGGGDK+FCLL
Sbjct: 121  RVRMVHCMEDWGLFKDAEAEGFRILERLRVIDRTS-----DCRVIHDRDKGGGDKEFCLL 180

Query: 181  FAEVVVTIVKCTASGRSKESGDYRRVLGLVEEVRPWFRLLDAKVSEKTQRALVTYLGKCT 240
            FAEVVVT+VKC ASGRSKESGDY R+LGLVEEVRPWFRLLDAKVSEKTQRAL TYLGKCT
Sbjct: 181  FAEVVVTLVKCAASGRSKESGDYGRLLGLVEEVRPWFRLLDAKVSEKTQRALATYLGKCT 240

Query: 241  IFLVEEVVCFGESLVSLFCRTTFAEYAKSSMRDQIYKLARRICSTLFSLQQEQHTSMLIM 300
            IFLVEE+  FGESLVSLFCR TFAEYAKSS++DQIYKLARRICSTLFSLQQEQH+SMLIM
Sbjct: 241  IFLVEELDYFGESLVSLFCRITFAEYAKSSLKDQIYKLARRICSTLFSLQQEQHSSMLIM 300

Query: 301  KILACVLKSITLEWKDETECTVIEFLQLICYCANKCQSESPECCCTFARHLEEMAADFHQ 360
             IL CV+KS+TLEWKDETE  V+EFLQLICY ANKC S SPECCC FA+HL+EMA +FHQ
Sbjct: 301  HILICVVKSLTLEWKDETEWEVVEFLQLICYSANKCLSTSPECCCAFAKHLQEMAGEFHQ 360

Query: 361  ATTPFGMNLRLYAAGLKIFSKVPRGETHGSAFSILVDDEDTMQSLVNLNGFLGSYFCIGC 420
            AT P GMNLRLYAAGLKI SK+PRGET GSAFS L+DDEDT+QSLVNLNGFLGSYFCIGC
Sbjct: 361  ATPPLGMNLRLYAAGLKIVSKLPRGETCGSAFSTLLDDEDTIQSLVNLNGFLGSYFCIGC 420

Query: 421  RKGNESCSNEQKDFVDQPCSHGKSNHECDVP-LLSWREAYLFSYLDAIKFLCQPLAESVN 480
            R+GNESCS EQKDFV QPC H  SNHE  VP LLS REAYL SYLDAIKF CQPLAESVN
Sbjct: 421  REGNESCSIEQKDFVGQPCLHRNSNHENGVPSLLSLREAYLSSYLDAIKFFCQPLAESVN 480

Query: 481  SERKEILAEDKAASVLYSIQNTLYQSCDVFLFCQRRAYDAKSDGCDENVNMLLSVVMAAF 540
             ERKEILAEDKAA VLY+IQNTL+Q CDVFLFCQRRAYDAKSDGCDENVNMLLS+V+AAF
Sbjct: 481  VERKEILAEDKAAPVLYNIQNTLHQFCDVFLFCQRRAYDAKSDGCDENVNMLLSIVVAAF 540

Query: 541  TLSIRTRLEMKRSTDLIKAVISSKWVQPFALKHLFASLNNIGIILYRNKQIREASKALKL 600
            TLS RTRL+MKRST+LIK VI+SKWVQPFALK LFASLNNIGIIL+RNKQI EASKALKL
Sbjct: 541  TLSFRTRLDMKRSTNLIKDVIASKWVQPFALKRLFASLNNIGIILFRNKQIGEASKALKL 600

Query: 601  CCRASWTCVRHFCVMFDDKAKPSDNEFSENAVLSFVNEVIMRSAFLVDILYQRGMHKVEK 660
            CCRASWTCV+ FC +FD+K++PSDNEFSEN+VL  VNEVIMRSAFLVDILYQ  M KVEK
Sbjct: 601  CCRASWTCVKQFCEIFDNKSRPSDNEFSENSVLIIVNEVIMRSAFLVDILYQCDMCKVEK 660

Query: 661  AVTEILKNWSMAEKLFNGLPVPVQLVKQWVKMQCKHHKNADPRKTTPTLNCLLQSSGMLS 720
            A+TEIL NWS A KLF+ LPVPVQLVKQWVKMQCK+HKNADP+K+TPTLNCLLQSS  +S
Sbjct: 661  AMTEILTNWSAAAKLFDELPVPVQLVKQWVKMQCKYHKNADPKKSTPTLNCLLQSSETVS 720

Query: 721  KAQIGALLEQELVEYAEMSGLYPEFCQSLQMKILSTLLRDVYVTPDHRLEKARVLLKKAR 780
            KA+IG LLEQELVEY EMSGLYPEFCQSLQMKILS LL D Y+TPDHRLEKARVL+KKAR
Sbjct: 721  KARIGVLLEQELVEYEEMSGLYPEFCQSLQMKILSILLTDTYITPDHRLEKARVLMKKAR 780

Query: 781  VLRFCETICLDNCIQCLSEAISTINETSGGTCSSGVLHSHQLAVAYCLRALCTHETVPNS 840
            VLR CET+CLD CIQCLSEAIST+NE S G+ S GVLHSHQLAVAYCLRA CTHETVPNS
Sbjct: 781  VLRLCETVCLDGCIQCLSEAISTMNEISSGSRSFGVLHSHQLAVAYCLRAFCTHETVPNS 840

Query: 841  KQVLQDIDSALSVWLEISSLDSLPDDQQLILSEYMLLLLCNTFDLLSIKGCMDYYHNIYS 900
            KQV QDIDSALS+WLEISSL++LPDDQ+LILSEYMLLLLCN FDLLSIKGC+DY+ NIYS
Sbjct: 841  KQVQQDIDSALSIWLEISSLNTLPDDQRLILSEYMLLLLCNAFDLLSIKGCVDYHDNIYS 900

Query: 901  LMIRLFKWKNVQLEKLLAVLWEGRRMSHAICIAPANEIVITQLSEHLGELPKSFNFWTNC 960
            LMIRLFKWKNV LEKL+A LWE RRMSHA+CIAPANEIVI QLSEHLG+LPKS +FWTNC
Sbjct: 901  LMIRLFKWKNVPLEKLMATLWESRRMSHAMCIAPANEIVIAQLSEHLGDLPKSNDFWTNC 960

Query: 961  LKTLPGMLVGFRQNFSFLCRNNGQSCCEHEKSVQLDVTVDEVKEAASKLISHTPACKSSI 1020
            LKTLPGMLVGF+QNFSFLC N  Q+ CEHEKS ++ VTVDEVKEAA KLISH PACKSSI
Sbjct: 961  LKTLPGMLVGFQQNFSFLCSNYTQTSCEHEKSSRMHVTVDEVKEAALKLISHIPACKSSI 1020

Query: 1021 FLAGYLYYDLCERLISEGCLTEALSCAKEAHRLRSKLFQEKFTYSIEQHPEKYCEISHVS 1080
            F AGYLYYDLCERLISEGCLTEAL CAKEAHRLRSKLFQEKFTYS+EQHPEKYCEI+H S
Sbjct: 1021 FSAGYLYYDLCERLISEGCLTEALLCAKEAHRLRSKLFQEKFTYSVEQHPEKYCEITHAS 1080

Query: 1081 QKPAYGIKNLQKNGLVARDVWSFDIISWDVEGCYLSPFNVLQCYLESTLQVGLVHEMIGN 1140
            QKP YGIKNL+K+G V+R++WSFD ISWDVEGCYLSPFNVLQCYLESTLQVGLVHE+IGN
Sbjct: 1081 QKPPYGIKNLRKSGSVSRNIWSFDKISWDVEGCYLSPFNVLQCYLESTLQVGLVHEIIGN 1140

Query: 1141 GSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLARKELEGAKEILTDSIT 1200
            GSEAETLLQWGKSISCL+SLPLFEVAFSSALGKVYRKKQLWSLA+KELEGAK+ILTD+IT
Sbjct: 1141 GSEAETLLQWGKSISCLRSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDNIT 1200

Query: 1201 SCLKCKLVLEVIVDQHLGDLFRSMYVNANGSISEGQLENAEELYKLALEKLNLSAWKNFI 1260
            SCLKC+LVLEVIVDQ+LGDLFRSMYVN NG+ SE  L NAEELYKLALEKLNLS WKN I
Sbjct: 1201 SCLKCRLVLEVIVDQYLGDLFRSMYVNGNGNTSEELLINAEELYKLALEKLNLSGWKNSI 1260

Query: 1261 SFPDEERYLSSLTIQAERPKARKDGRKCKKTTNASRPFQSDQCVNSQSNVRLTRSRCRTI 1320
            S PDE+ +LSSLTI  ER KARKDGRK KKTTNA R  + DQCVN Q+NVRLTRSRCR+I
Sbjct: 1261 SCPDEDSHLSSLTIPVERAKARKDGRKSKKTTNAPRSLKMDQCVNPQTNVRLTRSRCRSI 1320

Query: 1321 QGQSTSNSNEVEVDLSVHLKSNIPDLSSASCQKQSYLQVNSCTPTFGCGASCKNGKVGCW 1380
            QGQST+ SNEVEVDL+VHLKSN+PDL  AS QKQ +LQVN CTPT GCGASCKNGKVGCW
Sbjct: 1321 QGQSTNISNEVEVDLTVHLKSNVPDLFGASGQKQMHLQVNCCTPTLGCGASCKNGKVGCW 1380

Query: 1381 QCLPTEIMEAGEMNNFIYLKWEFVRRRLLLRHLSGLGKCSEIRGQIHQTHETILKSMSIL 1440
            QCL  EIMEAG++NNFIYLKWEFVRRRLLLR LSGLGKCS IRGQIHQTHETILKSMSIL
Sbjct: 1381 QCLQMEIMEAGQINNFIYLKWEFVRRRLLLRQLSGLGKCSGIRGQIHQTHETILKSMSIL 1440

Query: 1441 VSRNLFFQAHYAVEPMILLELVGKEVHRDMFAVERASVLYNICCFSLKSYKYDDSKITCC 1500
            VSRN+F QAH  VEP++LL+LVGKEV  DMFAVERASVLYNIC F+LKSYKYDD+KI CC
Sbjct: 1441 VSRNIFSQAHGVVEPVVLLDLVGKEVPGDMFAVERASVLYNICWFALKSYKYDDTKIICC 1500

Query: 1501 PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSSSELFSLPSSNKILTDS 1560
            PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSS EL SLPSSNKILTDS
Sbjct: 1501 PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSSIELLSLPSSNKILTDS 1560

Query: 1561 HWASYFHQASIGTHLNYQFFPYMTRRSCVEDLNFAKVLWNLLFFFVGFCRFLCCSSYNWI 1620
            HWASYFHQASIGTHLN+QFFPY T RSC++DLNFAK                        
Sbjct: 1561 HWASYFHQASIGTHLNHQFFPYTTGRSCIQDLNFAK------------------------ 1620

Query: 1621 VAYVIFWIFLFVYFSLYLFSFFFLLIVDACILHLKYGLIDGFNKVYFQGFDAGEQTLKLF 1680
                                                            GFD GEQT+KLF
Sbjct: 1621 ------------------------------------------------GFDTGEQTVKLF 1680

Query: 1681 RKGPFSSQDLEEHLRKFFDGLPCVTMVCISLIGGDLACLLQQILHFPSSVHAWILVSHLS 1740
            R+G FSSQDLEE++RKFFDGLPCV MVCISLIGGDLACLLQQILHFPSSVHAWILVSHL+
Sbjct: 1681 RRGSFSSQDLEEYVRKFFDGLPCVAMVCISLIGGDLACLLQQILHFPSSVHAWILVSHLN 1740

Query: 1741 SKRHPLVLLLPVETILKEGSEDYSNLQSDDSCERNDLAKHWQCPWGSSVIDEIAPAFRII 1800
            SKR PLVLLLPVETILK+ SEDYSN QSDD CERND  KHWQCPWGSSVIDEIAPAFR I
Sbjct: 1741 SKRQPLVLLLPVETILKD-SEDYSNPQSDDICERNDSTKHWQCPWGSSVIDEIAPAFRTI 1800

Query: 1801 LEENYLSSSVFPLEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYMLLGDWSTR 1860
            LE+NYLSSSVFP EDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKY+LLGDWS R
Sbjct: 1801 LEDNYLSSSVFPSEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYILLGDWSNR 1860

Query: 1861 EHFDSVLNTLVLNLKSKCKMDVNESLLKVILEGSEEVLEAFDSKLCSRKGCFIGKTRFYD 1920
            +H DSVLNTLVLNLKSKCKMDVNE LLK+ILEGSE VL  FDSKL SRKGCF+G+ RF+D
Sbjct: 1861 KHVDSVLNTLVLNLKSKCKMDVNEGLLKIILEGSENVLVGFDSKLYSRKGCFVGRARFHD 1920

Query: 1921 KERSNPFQNTLNGVDQLSALALKLIKDAKKELEGEENTCREPIILVLDYDVQMLPWENIP 1980
            KERSNPFQN LNGVD+LS LALKLI+DAKKELEGE+ T REPIILVLDYDVQMLPWENIP
Sbjct: 1921 KERSNPFQNALNGVDKLSTLALKLIQDAKKELEGEDGTSREPIILVLDYDVQMLPWENIP 1980

Query: 1981 ILQNQEVYRMPSVGCICATLDGRSHQQEQDGGIIAAFPSIDPLDAFYLLNPSGDLNSTQI 2040
            IL+NQEVYRMPSVG IC TLDGR HQQEQD GIIA FPSIDPLDAFYLLNPSGDL+STQI
Sbjct: 1981 ILKNQEVYRMPSVGSICVTLDGRLHQQEQDSGIIATFPSIDPLDAFYLLNPSGDLSSTQI 2040

Query: 2041 EFENWFKDQNLEGKAGYAPTSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS 2100
            EFENWFKDQNLEGKAGYAP SSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS
Sbjct: 2041 EFENWFKDQNLEGKAGYAPKSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS 2100

Query: 2101 LLMGCSSGSLTLNGHYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER 2160
            LLMGCSSGSLTLNG+YVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER
Sbjct: 2101 LLMGCSSGSLTLNGYYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER 2160

Query: 2161 SGALPSSAQCDIVTKEFEAMKISSKHANKKVASKSLPAACESESSSRGHSAHSRMIGSFL 2220
            S ALPSS QCDIVTKE EAMKISSK ANKKVASKSLP  CES SSS+ HS HSRMIGSFL
Sbjct: 2161 SCALPSSVQCDIVTKELEAMKISSKLANKKVASKSLPTTCES-SSSKDHSVHSRMIGSFL 2174

Query: 2221 CEAREACNLRYLIGASPVCYGVPTSIRKKKDLT 2253
            CEAREACNLRYLIGASPVCYGVPTSIRKKK+LT
Sbjct: 2221 CEAREACNLRYLIGASPVCYGVPTSIRKKKELT 2174

BLAST of Lcy11g005290 vs. ExPASy TrEMBL
Match: A0A6J1IQP1 (Separase OS=Cucurbita maxima OX=3661 GN=LOC111478544 PE=4 SV=1)

HSP 1 Score: 3732.6 bits (9678), Expect = 0.0e+00
Identity = 1878/2253 (83.36%), Postives = 1992/2253 (88.42%), Query Frame = 0

Query: 1    MASPSESSLISILETADSKGISSLVSDFLQPFSDVKNPKKCKKSAKSTDDSSTIRSLAKK 60
            MASPSE+S+ISILETADSKGI S VSDFL+PFSD+KNPKK KKSAK +DDSS IRSLAKK
Sbjct: 1    MASPSEASIISILETADSKGIFSTVSDFLRPFSDIKNPKKSKKSAKPSDDSSAIRSLAKK 60

Query: 61   FLAFLNRALSILPKRLSEPSKLGNDLELALEFFEVYKLCLGCLESLTSQLSCKPYTVDVQ 120
            FL FLNRALSILPKRLSEPSKLGNDLELALEFFE+YKLCL CLESLTSQLSCKPYTVDVQ
Sbjct: 61   FLVFLNRALSILPKRLSEPSKLGNDLELALEFFEIYKLCLACLESLTSQLSCKPYTVDVQ 120

Query: 121  RVRMVHCMEDWGLFKDAEAEGFGILERLREIDRKSKAGKLDCRVIHDGDKGGGDKDFCLL 180
            RVRMVH MEDWGLFKDAEAEGF ILERLR I R S     DCRVI D DKGGGDKDFCLL
Sbjct: 121  RVRMVHYMEDWGLFKDAEAEGFRILERLRVIGRTS-----DCRVIRDRDKGGGDKDFCLL 180

Query: 181  FAEVVVTIVKCTASGRSKESGDYRRVLGLVEEVRPWFRLLDAKVSEKTQRALVTYLGKCT 240
            FAEVVVT+VKC ASGRSKESG YRR+LGLVEEV PWFRLLDAKVSEKTQRAL TYLGKCT
Sbjct: 181  FAEVVVTLVKCAASGRSKESGAYRRLLGLVEEVGPWFRLLDAKVSEKTQRALATYLGKCT 240

Query: 241  IFLVEEVVCFGESLVSLFCRTTFAEYAKSSMRDQIYKLARRICSTLFSLQQEQHTSMLIM 300
            IFLVEE+  FGESL+SLFCR TFAEYAKSS+RDQIYKLARRICSTLFSLQQEQH+SMLIM
Sbjct: 241  IFLVEELDYFGESLISLFCRITFAEYAKSSLRDQIYKLARRICSTLFSLQQEQHSSMLIM 300

Query: 301  KILACVLKSITLEWKDETECTVIEFLQLICYCANKCQSESPECCCTFARHLEEMAADFHQ 360
             IL CVLKS+TLEWKDETE TV+EFLQLICY ANKC S SPECCC FA+HLEEMA +FH+
Sbjct: 301  HILICVLKSLTLEWKDETEWTVVEFLQLICYSANKCLSASPECCCAFAKHLEEMAREFHE 360

Query: 361  ATTPFGMNLRLYAAGLKIFSKVPRGETHGSAFSILVDDEDTMQSLVNLNGFLGSYFCIGC 420
            AT P GMNLRLYAAGLKI SK+PRGET GSAFS L+DDEDT+QSLVNLNGFLGSYFCIGC
Sbjct: 361  ATPPLGMNLRLYAAGLKIVSKLPRGETCGSAFSTLLDDEDTIQSLVNLNGFLGSYFCIGC 420

Query: 421  RKGNESCSNEQKDFVDQPCSHGKSNHECDVP-LLSWREAYLFSYLDAIKFLCQPLAESVN 480
            R+GNESCS EQKDFV QPC H   NHE  VP LLS RE YL SYLDAIKF CQPLAESVN
Sbjct: 421  REGNESCSIEQKDFVGQPCLHRNPNHENGVPSLLSLREGYLSSYLDAIKFFCQPLAESVN 480

Query: 481  SERKEILAEDKAASVLYSIQNTLYQSCDVFLFCQRRAYDAKSDGCDENVNMLLSVVMAAF 540
            +ERKEILAEDKA+ VLY+IQNTL+Q CDVFLFCQRRAYDAKSDGCDENVNMLLS+V+AAF
Sbjct: 481  AERKEILAEDKASPVLYNIQNTLHQFCDVFLFCQRRAYDAKSDGCDENVNMLLSIVVAAF 540

Query: 541  TLSIRTRLEMKRSTDLIKAVISSKWVQPFALKHLFASLNNIGIILYRNKQIREASKALKL 600
            TLS RTRLEMKRSTDLIK VI+SKWVQPFALK LFASLNNIGIIL+RN QI EASKALKL
Sbjct: 541  TLSFRTRLEMKRSTDLIKDVIASKWVQPFALKRLFASLNNIGIILFRNNQIGEASKALKL 600

Query: 601  CCRASWTCVRHFCVMFDDKAKPSDNEFSENAVLSFVNEVIMRSAFLVDILYQRGMHKVEK 660
            CCRASWTCV+ FC +FD+K++PSDNEFSEN+VL  VNEVIMRS+FLVDILYQRGM KVEK
Sbjct: 601  CCRASWTCVKQFCEIFDNKSRPSDNEFSENSVLIMVNEVIMRSSFLVDILYQRGMCKVEK 660

Query: 661  AVTEILKNWSMAEKLFNGLPVPVQLVKQWVKMQCKHHKNADPRKTTPTLNCLLQSSGMLS 720
            A+TEIL NWS A +LF+ LPVPVQLVKQWVKMQCK+HKNADP+K+TPTLNCLLQSS  +S
Sbjct: 661  AMTEILTNWSAATELFDELPVPVQLVKQWVKMQCKYHKNADPKKSTPTLNCLLQSSETVS 720

Query: 721  KAQIGALLEQELVEYAEMSGLYPEFCQSLQMKILSTLLRDVYVTPDHRLEKARVLLKKAR 780
            KA+IG LLEQELVEY EMSGLYPEFCQSLQMKILS LL D Y+TPDHRLEKARVL+KKAR
Sbjct: 721  KAKIGVLLEQELVEYEEMSGLYPEFCQSLQMKILSVLLTDTYITPDHRLEKARVLMKKAR 780

Query: 781  VLRFCETICLDNCIQCLSEAISTINETSGGTCSSGVLHSHQLAVAYCLRALCTHETVPNS 840
            VLR CET+CLD CIQCLSEAIST+NE SGG  S GVLHSHQLAVAYCLRA CTHETVPNS
Sbjct: 781  VLRLCETVCLDGCIQCLSEAISTMNEISGGCRSFGVLHSHQLAVAYCLRAFCTHETVPNS 840

Query: 841  KQVLQDIDSALSVWLEISSLDSLPDDQQLILSEYMLLLLCNTFDLLSIKGCMDYYHNIYS 900
            KQV QDIDSALS+WLEISSL++LPDDQ+LILSEYMLLLLCN FDLLSIKGC+DYY NIYS
Sbjct: 841  KQVQQDIDSALSIWLEISSLNTLPDDQRLILSEYMLLLLCNAFDLLSIKGCVDYYDNIYS 900

Query: 901  LMIRLFKWKNVQLEKLLAVLWEGRRMSHAICIAPANEIVITQLSEHLGELPKSFNFWTNC 960
            LMIRLFKWKNV LEKL+A LWE RRMSHA+CIAP NE+VI QLSEHLG+LPKS++FWTNC
Sbjct: 901  LMIRLFKWKNVPLEKLMATLWESRRMSHAMCIAPTNELVIAQLSEHLGDLPKSYDFWTNC 960

Query: 961  LKTLPGMLVGFRQNFSFLCRNNGQSCCEHEKSVQLDVTVDEVKEAASKLISHTPACKSSI 1020
            LKTLPGMLVGF+QNFSFLC N  Q+ CEHEKS ++ VTVDEVKEAA KLISH PACKSSI
Sbjct: 961  LKTLPGMLVGFQQNFSFLCSNYTQTSCEHEKSSRMHVTVDEVKEAALKLISHIPACKSSI 1020

Query: 1021 FLAGYLYYDLCERLISEGCLTEALSCAKEAHRLRSKLFQEKFTYSIEQHPEKYCEISHVS 1080
            FLAGYLYYDLCERLISEGCLTEAL CAKEAHRLRSKLFQEKFTYS+EQHPEKYCEI+H S
Sbjct: 1021 FLAGYLYYDLCERLISEGCLTEALLCAKEAHRLRSKLFQEKFTYSVEQHPEKYCEITHAS 1080

Query: 1081 QKPAYGIKNLQKNGLVARDVWSFDIISWDVEGCYLSPFNVLQCYLESTLQVGLVHEMIGN 1140
            QKP YGIKNL+K+G V+R++WSFD ISWDVEGCYLSPFN LQCYLESTLQVGLVHE+IGN
Sbjct: 1081 QKPPYGIKNLRKSGSVSRNIWSFDKISWDVEGCYLSPFNALQCYLESTLQVGLVHEIIGN 1140

Query: 1141 GSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLARKELEGAKEILTDSIT 1200
            GSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLA+KELEGAK+ILTD+IT
Sbjct: 1141 GSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDNIT 1200

Query: 1201 SCLKCKLVLEVIVDQHLGDLFRSMYVNANGSISEGQLENAEELYKLALEKLNLSAWKNFI 1260
            SCLKCKLVLEVIVDQ+LGDLFRSMYVN NG+ SE  L NAEELYKLALEKLNLS WKN I
Sbjct: 1201 SCLKCKLVLEVIVDQYLGDLFRSMYVNGNGNTSEELLINAEELYKLALEKLNLSGWKNSI 1260

Query: 1261 SFPDEERYLSSLTIQAERPKARKDGRKCKKTTNASRPFQSDQCVNSQSNVRLTRSRCRTI 1320
            S PDE  +LSSLTI  ER KARKDGRK KKTTN  R  Q D+CVN Q+NVRLTRSRCR+I
Sbjct: 1261 SCPDEASHLSSLTIPVERAKARKDGRKSKKTTNTPRSLQMDRCVNPQTNVRLTRSRCRSI 1320

Query: 1321 QGQSTSNSNEVEVDLSVHLKSNIPDLSSASCQKQSYLQVNSCTPTFGCGASCKNGKVGCW 1380
            QGQST+ SNEVEVDL+VHLKSN+PDLS AS QKQ +LQ+N CTPT GCGASCKNGKVGCW
Sbjct: 1321 QGQSTNISNEVEVDLAVHLKSNVPDLSGASGQKQMHLQLNCCTPTLGCGASCKNGKVGCW 1380

Query: 1381 QCLPTEIMEAGEMNNFIYLKWEFVRRRLLLRHLSGLGKCSEIRGQIHQTHETILKSMSIL 1440
            QCL  EIME G++NNFIYLKWEFVRRRLLLR LSGLGKCS IR QIHQTHETILK MSIL
Sbjct: 1381 QCLQMEIMETGQINNFIYLKWEFVRRRLLLRQLSGLGKCSGIRSQIHQTHETILKCMSIL 1440

Query: 1441 VSRNLFFQAHYAVEPMILLELVGKEVHRDMFAVERASVLYNICCFSLKSYKYDDSKITCC 1500
            VSRNLF QAH  VEP++LL+LVGKEV  DMFAVERASVLYNIC F+LKSYKYDD+KI CC
Sbjct: 1441 VSRNLFSQAHGVVEPVVLLDLVGKEVPGDMFAVERASVLYNICWFALKSYKYDDTKIICC 1500

Query: 1501 PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSSSELFSLPSSNKILTDS 1560
            PLSQVQSKTLVSWLMVAL LCCEVPVLFQKVSKLLAVIHVISSS EL SLPSSNKILTDS
Sbjct: 1501 PLSQVQSKTLVSWLMVALALCCEVPVLFQKVSKLLAVIHVISSSIELLSLPSSNKILTDS 1560

Query: 1561 HWASYFHQASIGTHLNYQFFPYMTRRSCVEDLNFAKVLWNLLFFFVGFCRFLCCSSYNWI 1620
            HWASYFHQASIGTHLN+QFFPY T RSC++DLNFAK                        
Sbjct: 1561 HWASYFHQASIGTHLNHQFFPYTTGRSCIQDLNFAK------------------------ 1620

Query: 1621 VAYVIFWIFLFVYFSLYLFSFFFLLIVDACILHLKYGLIDGFNKVYFQGFDAGEQTLKLF 1680
                                                            GFD GEQT+KLF
Sbjct: 1621 ------------------------------------------------GFDTGEQTVKLF 1680

Query: 1681 RKGPFSSQDLEEHLRKFFDGLPCVTMVCISLIGGDLACLLQQILHFPSSVHAWILVSHLS 1740
            R+G FSSQDLEE++RKFFDGLPCV MVCISLIGGDLACLLQQILHFPSSVHAWILVSHL+
Sbjct: 1681 RRGSFSSQDLEEYVRKFFDGLPCVAMVCISLIGGDLACLLQQILHFPSSVHAWILVSHLN 1740

Query: 1741 SKRHPLVLLLPVETILKEGSEDYSNLQSDDSCERNDLAKHWQCPWGSSVIDEIAPAFRII 1800
            SKR PLVLLLPVETILKE SEDYSN QSDD CERND  KHWQCPWGSSVIDEIAPAFR I
Sbjct: 1741 SKRQPLVLLLPVETILKEDSEDYSNPQSDDICERNDSTKHWQCPWGSSVIDEIAPAFRTI 1800

Query: 1801 LEENYLSSSVFPLEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYMLLGDWSTR 1860
            LEENYLSSSVFP EDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKY+LLGDWS R
Sbjct: 1801 LEENYLSSSVFPSEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYILLGDWSNR 1860

Query: 1861 EHFDSVLNTLVLNLKSKCKMDVNESLLKVILEGSEEVLEAFDSKLCSRKGCFIGKTRFYD 1920
            +H DSVLNTLVLNLKSKCKMDVNE LLK+ILEGSE VL  FDSKL SRKGCF+G+ RF+D
Sbjct: 1861 KHVDSVLNTLVLNLKSKCKMDVNEGLLKIILEGSENVLVGFDSKLYSRKGCFVGRARFHD 1920

Query: 1921 KERSNPFQNTLNGVDQLSALALKLIKDAKKELEGEENTCREPIILVLDYDVQMLPWENIP 1980
            KERSNPFQN LNGVD+LS LALKLI+DAKKELEGE+ T REPIILVLDYDVQMLPWENIP
Sbjct: 1921 KERSNPFQNALNGVDKLSTLALKLIQDAKKELEGEDGTSREPIILVLDYDVQMLPWENIP 1980

Query: 1981 ILQNQEVYRMPSVGCICATLDGRSHQQEQDGGIIAAFPSIDPLDAFYLLNPSGDLNSTQI 2040
            IL+NQEVYRMPSVG IC TLDGR HQQEQD GIIA FPSIDPLDAFYLLNPSGDL+STQI
Sbjct: 1981 ILKNQEVYRMPSVGSICVTLDGRLHQQEQDSGIIATFPSIDPLDAFYLLNPSGDLSSTQI 2040

Query: 2041 EFENWFKDQNLEGKAGYAPTSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS 2100
            EFENWFKDQNLEGKAGYAP SSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS
Sbjct: 2041 EFENWFKDQNLEGKAGYAPKSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS 2100

Query: 2101 LLMGCSSGSLTLNGHYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER 2160
            LLMGCSSGSLTLNG+YVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER
Sbjct: 2101 LLMGCSSGSLTLNGYYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER 2160

Query: 2161 SGALPSSAQCDIVTKEFEAMKISSKHANKKVASKSLPAACESESSSRGHSAHSRMIGSFL 2220
            S ALPSS Q DIVTKE EAMKISSK ANKKVASKSLP  CES +SSR HS  SRMIGSFL
Sbjct: 2161 SCALPSSVQYDIVTKELEAMKISSKRANKKVASKSLPTTCES-NSSRDHSVRSRMIGSFL 2175

Query: 2221 CEAREACNLRYLIGASPVCYGVPTSIRKKKDLT 2253
            CEAREACNLRYLIGASPVCYGVPTSIRKKK+LT
Sbjct: 2221 CEAREACNLRYLIGASPVCYGVPTSIRKKKELT 2175

BLAST of Lcy11g005290 vs. ExPASy TrEMBL
Match: A0A6J1ITU0 (Separase OS=Cucurbita maxima OX=3661 GN=LOC111478544 PE=4 SV=1)

HSP 1 Score: 3727.2 bits (9664), Expect = 0.0e+00
Identity = 1878/2253 (83.36%), Postives = 1991/2253 (88.37%), Query Frame = 0

Query: 1    MASPSESSLISILETADSKGISSLVSDFLQPFSDVKNPKKCKKSAKSTDDSSTIRSLAKK 60
            MASPSE+S+ISILETADSKGI S VSDFL+PFSD+KNPKK KKSAK +DDSS IRSLAKK
Sbjct: 1    MASPSEASIISILETADSKGIFSTVSDFLRPFSDIKNPKKSKKSAKPSDDSSAIRSLAKK 60

Query: 61   FLAFLNRALSILPKRLSEPSKLGNDLELALEFFEVYKLCLGCLESLTSQLSCKPYTVDVQ 120
            FL FLNRALSILPKRLSEPSKLGNDLELALEFFE+YKLCL CLESLTSQLSCKPYTVDVQ
Sbjct: 61   FLVFLNRALSILPKRLSEPSKLGNDLELALEFFEIYKLCLACLESLTSQLSCKPYTVDVQ 120

Query: 121  RVRMVHCMEDWGLFKDAEAEGFGILERLREIDRKSKAGKLDCRVIHDGDKGGGDKDFCLL 180
            RVRMVH MEDWGLFKDAEAEGF ILERLR I R S     DCRVI D DKGGGDKDFCLL
Sbjct: 121  RVRMVHYMEDWGLFKDAEAEGFRILERLRVIGRTS-----DCRVIRDRDKGGGDKDFCLL 180

Query: 181  FAEVVVTIVKCTASGRSKESGDYRRVLGLVEEVRPWFRLLDAKVSEKTQRALVTYLGKCT 240
            FAEVVVT+VKC ASGRSKESG YRR+LGLVEEV PWFRLLDAKVSEKTQRAL TYLGKCT
Sbjct: 181  FAEVVVTLVKCAASGRSKESGAYRRLLGLVEEVGPWFRLLDAKVSEKTQRALATYLGKCT 240

Query: 241  IFLVEEVVCFGESLVSLFCRTTFAEYAKSSMRDQIYKLARRICSTLFSLQQEQHTSMLIM 300
            IFLVEE+  FGESL+SLFCR TFAEYAKSS+RDQIYKLARRICSTLFSLQQEQH+SMLIM
Sbjct: 241  IFLVEELDYFGESLISLFCRITFAEYAKSSLRDQIYKLARRICSTLFSLQQEQHSSMLIM 300

Query: 301  KILACVLKSITLEWKDETECTVIEFLQLICYCANKCQSESPECCCTFARHLEEMAADFHQ 360
             IL CVLKS+TLEWKDETE TV+EFLQLICY ANKC S SPECCC FA+HLEEMA +FH+
Sbjct: 301  HILICVLKSLTLEWKDETEWTVVEFLQLICYSANKCLSASPECCCAFAKHLEEMAREFHE 360

Query: 361  ATTPFGMNLRLYAAGLKIFSKVPRGETHGSAFSILVDDEDTMQSLVNLNGFLGSYFCIGC 420
            AT P GMNLRLYAAGLKI SK+PRGET GSAFS L+DDEDT+QSLVNLNGFLGSYFCIGC
Sbjct: 361  ATPPLGMNLRLYAAGLKIVSKLPRGETCGSAFSTLLDDEDTIQSLVNLNGFLGSYFCIGC 420

Query: 421  RKGNESCSNEQKDFVDQPCSHGKSNHECDVP-LLSWREAYLFSYLDAIKFLCQPLAESVN 480
            R+GNESCS EQKDFV QPC H   NHE  VP LLS RE YL SYLDAIKF CQPLAESVN
Sbjct: 421  REGNESCSIEQKDFVGQPCLHRNPNHENGVPSLLSLREGYLSSYLDAIKFFCQPLAESVN 480

Query: 481  SERKEILAEDKAASVLYSIQNTLYQSCDVFLFCQRRAYDAKSDGCDENVNMLLSVVMAAF 540
            +ERKEILAEDKA+ VLY+IQNTL+Q CDVFLFCQRRAYDAKSDGCDENVNMLLS+V+AAF
Sbjct: 481  AERKEILAEDKASPVLYNIQNTLHQFCDVFLFCQRRAYDAKSDGCDENVNMLLSIVVAAF 540

Query: 541  TLSIRTRLEMKRSTDLIKAVISSKWVQPFALKHLFASLNNIGIILYRNKQIREASKALKL 600
            TLS RTRLEMKRSTDLIK VI+SKWVQPFALK LFASLNNIGIIL+RN QI EASKALKL
Sbjct: 541  TLSFRTRLEMKRSTDLIKDVIASKWVQPFALKRLFASLNNIGIILFRNNQIGEASKALKL 600

Query: 601  CCRASWTCVRHFCVMFDDKAKPSDNEFSENAVLSFVNEVIMRSAFLVDILYQRGMHKVEK 660
            CCRASWTCV+ FC +FD+K++PSDNEFSEN+VL  VNEVIMRS+FLVDILYQRGM KVEK
Sbjct: 601  CCRASWTCVKQFCEIFDNKSRPSDNEFSENSVLIMVNEVIMRSSFLVDILYQRGMCKVEK 660

Query: 661  AVTEILKNWSMAEKLFNGLPVPVQLVKQWVKMQCKHHKNADPRKTTPTLNCLLQSSGMLS 720
            A+TEIL NWS A +LF+ LPVPVQLVKQWVKMQCK+HKNADP+K+TPTLNCLLQSS  +S
Sbjct: 661  AMTEILTNWSAATELFDELPVPVQLVKQWVKMQCKYHKNADPKKSTPTLNCLLQSSETVS 720

Query: 721  KAQIGALLEQELVEYAEMSGLYPEFCQSLQMKILSTLLRDVYVTPDHRLEKARVLLKKAR 780
            KA+IG LLEQELVEY EMSGLYPEFCQSLQMKILS LL D Y+TPDHRLEKARVL+KKAR
Sbjct: 721  KAKIGVLLEQELVEYEEMSGLYPEFCQSLQMKILSVLLTDTYITPDHRLEKARVLMKKAR 780

Query: 781  VLRFCETICLDNCIQCLSEAISTINETSGGTCSSGVLHSHQLAVAYCLRALCTHETVPNS 840
            VLR CET+CLD CIQCLSEAIST+NE SGG  S GVLHSHQLAVAYCLRA CTHETVPNS
Sbjct: 781  VLRLCETVCLDGCIQCLSEAISTMNEISGGCRSFGVLHSHQLAVAYCLRAFCTHETVPNS 840

Query: 841  KQVLQDIDSALSVWLEISSLDSLPDDQQLILSEYMLLLLCNTFDLLSIKGCMDYYHNIYS 900
            KQV QDIDSALS+WLEISSL++LPDDQ+LILSEYMLLLLCN FDLLSIKGC+DYY NIYS
Sbjct: 841  KQVQQDIDSALSIWLEISSLNTLPDDQRLILSEYMLLLLCNAFDLLSIKGCVDYYDNIYS 900

Query: 901  LMIRLFKWKNVQLEKLLAVLWEGRRMSHAICIAPANEIVITQLSEHLGELPKSFNFWTNC 960
            LMIRLFKWKNV LEKL+A LWE RRMSHA+CIAP NE+VI QLSEHLG+LPKS++FWTNC
Sbjct: 901  LMIRLFKWKNVPLEKLMATLWESRRMSHAMCIAPTNELVIAQLSEHLGDLPKSYDFWTNC 960

Query: 961  LKTLPGMLVGFRQNFSFLCRNNGQSCCEHEKSVQLDVTVDEVKEAASKLISHTPACKSSI 1020
            LKTLPGMLVGF+QNFSFLC N  Q+ CEHEKS ++ VTVDEVKEAA KLISH PACKSSI
Sbjct: 961  LKTLPGMLVGFQQNFSFLCSNYTQTSCEHEKSSRMHVTVDEVKEAALKLISHIPACKSSI 1020

Query: 1021 FLAGYLYYDLCERLISEGCLTEALSCAKEAHRLRSKLFQEKFTYSIEQHPEKYCEISHVS 1080
            FLAGYLYYDLCERLISEGCLTEAL CAKEAHRLRSKLFQEKFTYS+EQHPEKYCEI+H S
Sbjct: 1021 FLAGYLYYDLCERLISEGCLTEALLCAKEAHRLRSKLFQEKFTYSVEQHPEKYCEITHAS 1080

Query: 1081 QKPAYGIKNLQKNGLVARDVWSFDIISWDVEGCYLSPFNVLQCYLESTLQVGLVHEMIGN 1140
            QKP YGIKNL+K+G V+R++WSFD ISWDVEGCYLSPFN LQCYLESTLQVGLVHE+IGN
Sbjct: 1081 QKPPYGIKNLRKSGSVSRNIWSFDKISWDVEGCYLSPFNALQCYLESTLQVGLVHEIIGN 1140

Query: 1141 GSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLARKELEGAKEILTDSIT 1200
            GSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLA+KELEGAK+ILTD+IT
Sbjct: 1141 GSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDNIT 1200

Query: 1201 SCLKCKLVLEVIVDQHLGDLFRSMYVNANGSISEGQLENAEELYKLALEKLNLSAWKNFI 1260
            SCLKCKLVLEVIVDQ+LGDLFRSMYVN NG+ SE  L NAEELYKLALEKLNLS WKN I
Sbjct: 1201 SCLKCKLVLEVIVDQYLGDLFRSMYVNGNGNTSEELLINAEELYKLALEKLNLSGWKNSI 1260

Query: 1261 SFPDEERYLSSLTIQAERPKARKDGRKCKKTTNASRPFQSDQCVNSQSNVRLTRSRCRTI 1320
            S PDE  +LSSLTI  ER KARKDGRK KKTTN  R  Q D+CVN Q+NVRLTRSRCR+I
Sbjct: 1261 SCPDEASHLSSLTIPVERAKARKDGRKSKKTTNTPRSLQMDRCVNPQTNVRLTRSRCRSI 1320

Query: 1321 QGQSTSNSNEVEVDLSVHLKSNIPDLSSASCQKQSYLQVNSCTPTFGCGASCKNGKVGCW 1380
            QGQST+ SNEVEVDL+VHLKSN+PDLS AS QKQ +LQ+N CTPT GCGASCKNGKVGCW
Sbjct: 1321 QGQSTNISNEVEVDLAVHLKSNVPDLSGASGQKQMHLQLNCCTPTLGCGASCKNGKVGCW 1380

Query: 1381 QCLPTEIMEAGEMNNFIYLKWEFVRRRLLLRHLSGLGKCSEIRGQIHQTHETILKSMSIL 1440
            QCL  EIME G++NNFIYLKWEFVRRRLLLR LSGLGKCS IR QIHQTHETILK MSIL
Sbjct: 1381 QCLQMEIMETGQINNFIYLKWEFVRRRLLLRQLSGLGKCSGIRSQIHQTHETILKCMSIL 1440

Query: 1441 VSRNLFFQAHYAVEPMILLELVGKEVHRDMFAVERASVLYNICCFSLKSYKYDDSKITCC 1500
            VSRNLF QAH  VEP++LL+LVGKEV  DMFAVERASVLYNIC F+LKSYKYDD+KI CC
Sbjct: 1441 VSRNLFSQAHGVVEPVVLLDLVGKEVPGDMFAVERASVLYNICWFALKSYKYDDTKIICC 1500

Query: 1501 PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSSSELFSLPSSNKILTDS 1560
            PLSQVQSKTLVSWLMVAL LCCEVPVLFQKVSKLLAVIHVISSS EL SLPSSNKILTDS
Sbjct: 1501 PLSQVQSKTLVSWLMVALALCCEVPVLFQKVSKLLAVIHVISSSIELLSLPSSNKILTDS 1560

Query: 1561 HWASYFHQASIGTHLNYQFFPYMTRRSCVEDLNFAKVLWNLLFFFVGFCRFLCCSSYNWI 1620
            HWASYFHQASIGTHLN+QFFPY T RSC++DLNFAK                        
Sbjct: 1561 HWASYFHQASIGTHLNHQFFPYTTGRSCIQDLNFAK------------------------ 1620

Query: 1621 VAYVIFWIFLFVYFSLYLFSFFFLLIVDACILHLKYGLIDGFNKVYFQGFDAGEQTLKLF 1680
                                                            GFD GEQT+KLF
Sbjct: 1621 ------------------------------------------------GFDTGEQTVKLF 1680

Query: 1681 RKGPFSSQDLEEHLRKFFDGLPCVTMVCISLIGGDLACLLQQILHFPSSVHAWILVSHLS 1740
            R G FSSQDLEE++RKFFDGLPCV MVCISLIGGDLACLLQQILHFPSSVHAWILVSHL+
Sbjct: 1681 R-GSFSSQDLEEYVRKFFDGLPCVAMVCISLIGGDLACLLQQILHFPSSVHAWILVSHLN 1740

Query: 1741 SKRHPLVLLLPVETILKEGSEDYSNLQSDDSCERNDLAKHWQCPWGSSVIDEIAPAFRII 1800
            SKR PLVLLLPVETILKE SEDYSN QSDD CERND  KHWQCPWGSSVIDEIAPAFR I
Sbjct: 1741 SKRQPLVLLLPVETILKEDSEDYSNPQSDDICERNDSTKHWQCPWGSSVIDEIAPAFRTI 1800

Query: 1801 LEENYLSSSVFPLEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYMLLGDWSTR 1860
            LEENYLSSSVFP EDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKY+LLGDWS R
Sbjct: 1801 LEENYLSSSVFPSEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYILLGDWSNR 1860

Query: 1861 EHFDSVLNTLVLNLKSKCKMDVNESLLKVILEGSEEVLEAFDSKLCSRKGCFIGKTRFYD 1920
            +H DSVLNTLVLNLKSKCKMDVNE LLK+ILEGSE VL  FDSKL SRKGCF+G+ RF+D
Sbjct: 1861 KHVDSVLNTLVLNLKSKCKMDVNEGLLKIILEGSENVLVGFDSKLYSRKGCFVGRARFHD 1920

Query: 1921 KERSNPFQNTLNGVDQLSALALKLIKDAKKELEGEENTCREPIILVLDYDVQMLPWENIP 1980
            KERSNPFQN LNGVD+LS LALKLI+DAKKELEGE+ T REPIILVLDYDVQMLPWENIP
Sbjct: 1921 KERSNPFQNALNGVDKLSTLALKLIQDAKKELEGEDGTSREPIILVLDYDVQMLPWENIP 1980

Query: 1981 ILQNQEVYRMPSVGCICATLDGRSHQQEQDGGIIAAFPSIDPLDAFYLLNPSGDLNSTQI 2040
            IL+NQEVYRMPSVG IC TLDGR HQQEQD GIIA FPSIDPLDAFYLLNPSGDL+STQI
Sbjct: 1981 ILKNQEVYRMPSVGSICVTLDGRLHQQEQDSGIIATFPSIDPLDAFYLLNPSGDLSSTQI 2040

Query: 2041 EFENWFKDQNLEGKAGYAPTSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS 2100
            EFENWFKDQNLEGKAGYAP SSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS
Sbjct: 2041 EFENWFKDQNLEGKAGYAPKSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS 2100

Query: 2101 LLMGCSSGSLTLNGHYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER 2160
            LLMGCSSGSLTLNG+YVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER
Sbjct: 2101 LLMGCSSGSLTLNGYYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER 2160

Query: 2161 SGALPSSAQCDIVTKEFEAMKISSKHANKKVASKSLPAACESESSSRGHSAHSRMIGSFL 2220
            S ALPSS Q DIVTKE EAMKISSK ANKKVASKSLP  CES +SSR HS  SRMIGSFL
Sbjct: 2161 SCALPSSVQYDIVTKELEAMKISSKRANKKVASKSLPTTCES-NSSRDHSVRSRMIGSFL 2174

Query: 2221 CEAREACNLRYLIGASPVCYGVPTSIRKKKDLT 2253
            CEAREACNLRYLIGASPVCYGVPTSIRKKK+LT
Sbjct: 2221 CEAREACNLRYLIGASPVCYGVPTSIRKKKELT 2174

BLAST of Lcy11g005290 vs. NCBI nr
Match: XP_023533736.1 (separase isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 3756.8 bits (9741), Expect = 0.0e+00
Identity = 1891/2253 (83.93%), Postives = 2003/2253 (88.90%), Query Frame = 0

Query: 1    MASPSESSLISILETADSKGISSLVSDFLQPFSDVKNPKKCKKSAKSTDDSSTIRSLAKK 60
            MASPSE+S+ISILETADSKGI S VSDFL+PFSD+KNPKK KKSAK +DDSS IRSLAKK
Sbjct: 1    MASPSEASIISILETADSKGIFSTVSDFLRPFSDIKNPKKSKKSAKPSDDSSAIRSLAKK 60

Query: 61   FLAFLNRALSILPKRLSEPSKLGNDLELALEFFEVYKLCLGCLESLTSQLSCKPYTVDVQ 120
            FL FLNRALSILPKRLSEPSKLGNDLELALEFFE+YKLCL CLESLTSQLSCKPYTVDVQ
Sbjct: 61   FLTFLNRALSILPKRLSEPSKLGNDLELALEFFEIYKLCLACLESLTSQLSCKPYTVDVQ 120

Query: 121  RVRMVHCMEDWGLFKDAEAEGFGILERLREIDRKSKAGKLDCRVIHDGDKGGGDKDFCLL 180
            RVRMVHCMEDWGLFKDAEAEGF ILERLR IDR S     DCRVIHD DKGGGDKDFCLL
Sbjct: 121  RVRMVHCMEDWGLFKDAEAEGFRILERLRVIDRTS-----DCRVIHDRDKGGGDKDFCLL 180

Query: 181  FAEVVVTIVKCTASGRSKESGDYRRVLGLVEEVRPWFRLLDAKVSEKTQRALVTYLGKCT 240
            FAEVVVT+VKC ASGRSKESGDYRR+LGLVEEVRPWFRLLDAKVSEKTQRAL TYLGKCT
Sbjct: 181  FAEVVVTLVKCAASGRSKESGDYRRLLGLVEEVRPWFRLLDAKVSEKTQRALATYLGKCT 240

Query: 241  IFLVEEVVCFGESLVSLFCRTTFAEYAKSSMRDQIYKLARRICSTLFSLQQEQHTSMLIM 300
            IFLVEE+  FGESLVSLFCR TFAEYAKSS++DQIYKLARRICSTLFSLQQEQH+SMLIM
Sbjct: 241  IFLVEELDYFGESLVSLFCRITFAEYAKSSLKDQIYKLARRICSTLFSLQQEQHSSMLIM 300

Query: 301  KILACVLKSITLEWKDETECTVIEFLQLICYCANKCQSESPECCCTFARHLEEMAADFHQ 360
             IL CVLKS+TLEWKDETE  V+EFLQLICY ANKC S SPECCC FA+HLEEMA +FHQ
Sbjct: 301  HILICVLKSLTLEWKDETELEVVEFLQLICYSANKCLSASPECCCAFAKHLEEMAGEFHQ 360

Query: 361  ATTPFGMNLRLYAAGLKIFSKVPRGETHGSAFSILVDDEDTMQSLVNLNGFLGSYFCIGC 420
            AT P GMNLRLYAAGLKI SK+PRGET GSAFS L+DDEDT+QSLVNLNGFLGSYFCIGC
Sbjct: 361  ATPPLGMNLRLYAAGLKIVSKLPRGETCGSAFSTLLDDEDTIQSLVNLNGFLGSYFCIGC 420

Query: 421  RKGNESCSNEQKDFVDQPCSHGKSNHECDVP-LLSWREAYLFSYLDAIKFLCQPLAESVN 480
            R+GNESCS EQKDFV QPC H  SNHE  VP LLS REAYL SYLDAIKF CQPLAESVN
Sbjct: 421  REGNESCSIEQKDFVGQPCLHRNSNHENGVPSLLSLREAYLSSYLDAIKFFCQPLAESVN 480

Query: 481  SERKEILAEDKAASVLYSIQNTLYQSCDVFLFCQRRAYDAKSDGCDENVNMLLSVVMAAF 540
            +ERKEILAEDKA  VLY+IQNTL+Q CDVFLFCQRRAYDAKSDGCDENVNMLLS+V+AAF
Sbjct: 481  AERKEILAEDKAFPVLYNIQNTLHQFCDVFLFCQRRAYDAKSDGCDENVNMLLSIVVAAF 540

Query: 541  TLSIRTRLEMKRSTDLIKAVISSKWVQPFALKHLFASLNNIGIILYRNKQIREASKALKL 600
            TLS RTRLEMKRSTDLIK VISSKWVQPFALK LFASLNNIGIIL+RNKQI EASKALKL
Sbjct: 541  TLSFRTRLEMKRSTDLIKDVISSKWVQPFALKRLFASLNNIGIILFRNKQIGEASKALKL 600

Query: 601  CCRASWTCVRHFCVMFDDKAKPSDNEFSENAVLSFVNEVIMRSAFLVDILYQRGMHKVEK 660
            CCRASWTCV+ FC +F DK++PSDNEFSEN+VL   NEVIMRSAFLVDILYQRGM KVEK
Sbjct: 601  CCRASWTCVKQFCEIF-DKSRPSDNEFSENSVLIMFNEVIMRSAFLVDILYQRGMCKVEK 660

Query: 661  AVTEILKNWSMAEKLFNGLPVPVQLVKQWVKMQCKHHKNADPRKTTPTLNCLLQSSGMLS 720
            A+TEIL NWS A KLF+ LP PVQLVKQWVKMQCK+HKNADP+K+TPTLNCLLQSS  +S
Sbjct: 661  AMTEILTNWSAAAKLFDELPAPVQLVKQWVKMQCKYHKNADPKKSTPTLNCLLQSSETVS 720

Query: 721  KAQIGALLEQELVEYAEMSGLYPEFCQSLQMKILSTLLRDVYVTPDHRLEKARVLLKKAR 780
            KA+IG LLEQELVEY EMSGLYPEFCQSLQMKILS LL D Y+TPDHRLEKARVL+KKAR
Sbjct: 721  KAKIGVLLEQELVEYEEMSGLYPEFCQSLQMKILSILLTDTYITPDHRLEKARVLMKKAR 780

Query: 781  VLRFCETICLDNCIQCLSEAISTINETSGGTCSSGVLHSHQLAVAYCLRALCTHETVPNS 840
            VLR CET+CLD CIQCLSEAIST+NE SGG+ S GVLHSHQLAVAYCLRA CTHETVPNS
Sbjct: 781  VLRLCETVCLDGCIQCLSEAISTMNEISGGSRSFGVLHSHQLAVAYCLRAFCTHETVPNS 840

Query: 841  KQVLQDIDSALSVWLEISSLDSLPDDQQLILSEYMLLLLCNTFDLLSIKGCMDYYHNIYS 900
            KQV QDIDSALS+WLEISSL++LPDDQ+LILSEYMLLLLCN FDLLSIKGC+DYY NIYS
Sbjct: 841  KQVQQDIDSALSIWLEISSLNTLPDDQRLILSEYMLLLLCNAFDLLSIKGCVDYYDNIYS 900

Query: 901  LMIRLFKWKNVQLEKLLAVLWEGRRMSHAICIAPANEIVITQLSEHLGELPKSFNFWTNC 960
            LMIRLFKWKNV LEKL+A LWE RRMSHA+CIAPANE+VI QLSEHLG+LPKS++FWTNC
Sbjct: 901  LMIRLFKWKNVPLEKLMATLWESRRMSHAMCIAPANELVIAQLSEHLGDLPKSYDFWTNC 960

Query: 961  LKTLPGMLVGFRQNFSFLCRNNGQSCCEHEKSVQLDVTVDEVKEAASKLISHTPACKSSI 1020
            LKTLPGMLVGF+QNFSFLC N  Q+ CEHEKS ++ VTVDEVKEAA KLISH PACKSSI
Sbjct: 961  LKTLPGMLVGFQQNFSFLCSNYTQTSCEHEKSSRMHVTVDEVKEAALKLISHIPACKSSI 1020

Query: 1021 FLAGYLYYDLCERLISEGCLTEALSCAKEAHRLRSKLFQEKFTYSIEQHPEKYCEISHVS 1080
            F AGYLYYDLCERLISEGCLTEAL CAKEAHRLRSKLFQEKFTYS+EQHPEK+CEI+H S
Sbjct: 1021 FSAGYLYYDLCERLISEGCLTEALLCAKEAHRLRSKLFQEKFTYSVEQHPEKFCEITHAS 1080

Query: 1081 QKPAYGIKNLQKNGLVARDVWSFDIISWDVEGCYLSPFNVLQCYLESTLQVGLVHEMIGN 1140
            QKP YGIKNL+K+G V+R++WSFD ISWDVEGCYLSPFNVLQCYLESTLQVGLVHE+IGN
Sbjct: 1081 QKPPYGIKNLRKSGSVSRNIWSFDKISWDVEGCYLSPFNVLQCYLESTLQVGLVHEIIGN 1140

Query: 1141 GSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLARKELEGAKEILTDSIT 1200
            GSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLA+KELEGAK+ILTD+IT
Sbjct: 1141 GSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDNIT 1200

Query: 1201 SCLKCKLVLEVIVDQHLGDLFRSMYVNANGSISEGQLENAEELYKLALEKLNLSAWKNFI 1260
            SCLKC+LVLEVIVDQ+LGDLFRSMYVN NG+ SE  L NAEELYKLALEKLNLS WKN I
Sbjct: 1201 SCLKCRLVLEVIVDQYLGDLFRSMYVNGNGNTSEELLINAEELYKLALEKLNLSGWKNSI 1260

Query: 1261 SFPDEERYLSSLTIQAERPKARKDGRKCKKTTNASRPFQSDQCVNSQSNVRLTRSRCRTI 1320
            S PDEE +LSSLTI  ER KARKDGRK KKTTNA R  Q DQCVN Q+NVRLTRSRCR+I
Sbjct: 1261 SCPDEESHLSSLTIPVERAKARKDGRKSKKTTNAPRSLQMDQCVNPQTNVRLTRSRCRSI 1320

Query: 1321 QGQSTSNSNEVEVDLSVHLKSNIPDLSSASCQKQSYLQVNSCTPTFGCGASCKNGKVGCW 1380
            QGQST+ SNEVEVDL+VHLKSN+PDLS AS QKQ +LQVN CTPT GCGASCKNGKVGCW
Sbjct: 1321 QGQSTNISNEVEVDLTVHLKSNVPDLSGASGQKQMHLQVNCCTPTLGCGASCKNGKVGCW 1380

Query: 1381 QCLPTEIMEAGEMNNFIYLKWEFVRRRLLLRHLSGLGKCSEIRGQIHQTHETILKSMSIL 1440
            QCL  EIMEAG++NNFIYLKWEF RRRLLLR LSGLGKCS IRGQIHQTHETILKSMSIL
Sbjct: 1381 QCLQMEIMEAGQINNFIYLKWEFARRRLLLRQLSGLGKCSGIRGQIHQTHETILKSMSIL 1440

Query: 1441 VSRNLFFQAHYAVEPMILLELVGKEVHRDMFAVERASVLYNICCFSLKSYKYDDSKITCC 1500
            VSRNLF QAH  VEP++LL+LVGKEV  DMFAVERASVLYNIC F+LKSYKYDD+KI CC
Sbjct: 1441 VSRNLFSQAHGVVEPVVLLDLVGKEVPGDMFAVERASVLYNICWFALKSYKYDDTKIICC 1500

Query: 1501 PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSSSELFSLPSSNKILTDS 1560
            PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSS EL SLPSSNKILTDS
Sbjct: 1501 PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSSIELLSLPSSNKILTDS 1560

Query: 1561 HWASYFHQASIGTHLNYQFFPYMTRRSCVEDLNFAKVLWNLLFFFVGFCRFLCCSSYNWI 1620
            HWASYFHQASIGTHLN+QFFPY T RSC++DLNFAK                        
Sbjct: 1561 HWASYFHQASIGTHLNHQFFPYTTGRSCIQDLNFAK------------------------ 1620

Query: 1621 VAYVIFWIFLFVYFSLYLFSFFFLLIVDACILHLKYGLIDGFNKVYFQGFDAGEQTLKLF 1680
                                                            GFD GEQT+KLF
Sbjct: 1621 ------------------------------------------------GFDTGEQTVKLF 1680

Query: 1681 RKGPFSSQDLEEHLRKFFDGLPCVTMVCISLIGGDLACLLQQILHFPSSVHAWILVSHLS 1740
            R+G FSSQDLEE++RKFFDGLPCV MVCISLIGGDLACLLQQILHFPSSVHAWILVSHL+
Sbjct: 1681 RRGSFSSQDLEEYVRKFFDGLPCVAMVCISLIGGDLACLLQQILHFPSSVHAWILVSHLN 1740

Query: 1741 SKRHPLVLLLPVETILKEGSEDYSNLQSDDSCERNDLAKHWQCPWGSSVIDEIAPAFRII 1800
            SKR PLVLLLPVETILKE SE YSN QSDD CERND  KHWQCPWGSSVIDEIAP+FR I
Sbjct: 1741 SKRQPLVLLLPVETILKEDSEAYSNPQSDDICERNDSTKHWQCPWGSSVIDEIAPSFRTI 1800

Query: 1801 LEENYLSSSVFPLEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYMLLGDWSTR 1860
            LEENYLSSSVFP EDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKY+LLG WS R
Sbjct: 1801 LEENYLSSSVFPSEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYILLGGWSNR 1860

Query: 1861 EHFDSVLNTLVLNLKSKCKMDVNESLLKVILEGSEEVLEAFDSKLCSRKGCFIGKTRFYD 1920
            +H DSVLNTLVLNLKSKCKMDVNE LLK+ILEGSE+VL  FDSKL  RKGCF+G+ RFYD
Sbjct: 1861 KHVDSVLNTLVLNLKSKCKMDVNEGLLKIILEGSEDVLVGFDSKLYWRKGCFVGRARFYD 1920

Query: 1921 KERSNPFQNTLNGVDQLSALALKLIKDAKKELEGEENTCREPIILVLDYDVQMLPWENIP 1980
            KE+SNPFQN LNGVD+LS LALKLI+DAKKELEGE+ T REPIILVLDYDVQMLPWENIP
Sbjct: 1921 KEKSNPFQNALNGVDKLSTLALKLIQDAKKELEGEDGTSREPIILVLDYDVQMLPWENIP 1980

Query: 1981 ILQNQEVYRMPSVGCICATLDGRSHQQEQDGGIIAAFPSIDPLDAFYLLNPSGDLNSTQI 2040
            IL+NQEVYRMPSVG IC TLDGR HQQEQD GIIA FPSIDPLDAFYLLNPSGDL+STQI
Sbjct: 1981 ILKNQEVYRMPSVGSICVTLDGRLHQQEQDSGIIATFPSIDPLDAFYLLNPSGDLSSTQI 2040

Query: 2041 EFENWFKDQNLEGKAGYAPTSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS 2100
            EFENWFKDQNLEGKAGYAP SSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS
Sbjct: 2041 EFENWFKDQNLEGKAGYAPKSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS 2100

Query: 2101 LLMGCSSGSLTLNGHYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER 2160
            LLMGCSSGSLTLNG+YVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER
Sbjct: 2101 LLMGCSSGSLTLNGYYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER 2160

Query: 2161 SGALPSSAQCDIVTKEFEAMKISSKHANKKVASKSLPAACESESSSRGHSAHSRMIGSFL 2220
            S +LPSS QCDIVTKE EAMKISSK ANKKVASKSLP ACES +SSR HS HSRMIGSFL
Sbjct: 2161 SCSLPSSVQCDIVTKELEAMKISSKRANKKVASKSLPTACES-TSSRDHSVHSRMIGSFL 2174

Query: 2221 CEAREACNLRYLIGASPVCYGVPTSIRKKKDLT 2253
            CEAREACNLRYLIGASPVCYGVPTSIRKKK+LT
Sbjct: 2221 CEAREACNLRYLIGASPVCYGVPTSIRKKKELT 2174

BLAST of Lcy11g005290 vs. NCBI nr
Match: XP_023533753.1 (separase isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 3751.4 bits (9727), Expect = 0.0e+00
Identity = 1891/2253 (83.93%), Postives = 2002/2253 (88.86%), Query Frame = 0

Query: 1    MASPSESSLISILETADSKGISSLVSDFLQPFSDVKNPKKCKKSAKSTDDSSTIRSLAKK 60
            MASPSE+S+ISILETADSKGI S VSDFL+PFSD+KNPKK KKSAK +DDSS IRSLAKK
Sbjct: 1    MASPSEASIISILETADSKGIFSTVSDFLRPFSDIKNPKKSKKSAKPSDDSSAIRSLAKK 60

Query: 61   FLAFLNRALSILPKRLSEPSKLGNDLELALEFFEVYKLCLGCLESLTSQLSCKPYTVDVQ 120
            FL FLNRALSILPKRLSEPSKLGNDLELALEFFE+YKLCL CLESLTSQLSCKPYTVDVQ
Sbjct: 61   FLTFLNRALSILPKRLSEPSKLGNDLELALEFFEIYKLCLACLESLTSQLSCKPYTVDVQ 120

Query: 121  RVRMVHCMEDWGLFKDAEAEGFGILERLREIDRKSKAGKLDCRVIHDGDKGGGDKDFCLL 180
            RVRMVHCMEDWGLFKDAEAEGF ILERLR IDR S     DCRVIHD DKGGGDKDFCLL
Sbjct: 121  RVRMVHCMEDWGLFKDAEAEGFRILERLRVIDRTS-----DCRVIHDRDKGGGDKDFCLL 180

Query: 181  FAEVVVTIVKCTASGRSKESGDYRRVLGLVEEVRPWFRLLDAKVSEKTQRALVTYLGKCT 240
            FAEVVVT+VKC ASGRSKESGDYRR+LGLVEEVRPWFRLLDAKVSEKTQRAL TYLGKCT
Sbjct: 181  FAEVVVTLVKCAASGRSKESGDYRRLLGLVEEVRPWFRLLDAKVSEKTQRALATYLGKCT 240

Query: 241  IFLVEEVVCFGESLVSLFCRTTFAEYAKSSMRDQIYKLARRICSTLFSLQQEQHTSMLIM 300
            IFLVEE+  FGESLVSLFCR TFAEYAKSS++DQIYKLARRICSTLFSLQQEQH+SMLIM
Sbjct: 241  IFLVEELDYFGESLVSLFCRITFAEYAKSSLKDQIYKLARRICSTLFSLQQEQHSSMLIM 300

Query: 301  KILACVLKSITLEWKDETECTVIEFLQLICYCANKCQSESPECCCTFARHLEEMAADFHQ 360
             IL CVLKS+TLEWKDETE  V+EFLQLICY ANKC S SPECCC FA+HLEEMA +FHQ
Sbjct: 301  HILICVLKSLTLEWKDETELEVVEFLQLICYSANKCLSASPECCCAFAKHLEEMAGEFHQ 360

Query: 361  ATTPFGMNLRLYAAGLKIFSKVPRGETHGSAFSILVDDEDTMQSLVNLNGFLGSYFCIGC 420
            AT P GMNLRLYAAGLKI SK+PRGET GSAFS L+DDEDT+QSLVNLNGFLGSYFCIGC
Sbjct: 361  ATPPLGMNLRLYAAGLKIVSKLPRGETCGSAFSTLLDDEDTIQSLVNLNGFLGSYFCIGC 420

Query: 421  RKGNESCSNEQKDFVDQPCSHGKSNHECDVP-LLSWREAYLFSYLDAIKFLCQPLAESVN 480
            R+GNESCS EQKDFV QPC H  SNHE  VP LLS REAYL SYLDAIKF CQPLAESVN
Sbjct: 421  REGNESCSIEQKDFVGQPCLHRNSNHENGVPSLLSLREAYLSSYLDAIKFFCQPLAESVN 480

Query: 481  SERKEILAEDKAASVLYSIQNTLYQSCDVFLFCQRRAYDAKSDGCDENVNMLLSVVMAAF 540
            +ERKEILAEDKA  VLY+IQNTL+Q CDVFLFCQRRAYDAKSDGCDENVNMLLS+V+AAF
Sbjct: 481  AERKEILAEDKAFPVLYNIQNTLHQFCDVFLFCQRRAYDAKSDGCDENVNMLLSIVVAAF 540

Query: 541  TLSIRTRLEMKRSTDLIKAVISSKWVQPFALKHLFASLNNIGIILYRNKQIREASKALKL 600
            TLS RTRLEMKRSTDLIK VISSKWVQPFALK LFASLNNIGIIL+RNKQI EASKALKL
Sbjct: 541  TLSFRTRLEMKRSTDLIKDVISSKWVQPFALKRLFASLNNIGIILFRNKQIGEASKALKL 600

Query: 601  CCRASWTCVRHFCVMFDDKAKPSDNEFSENAVLSFVNEVIMRSAFLVDILYQRGMHKVEK 660
            CCRASWTCV+ FC +F DK++PSDNEFSEN+VL   NEVIMRSAFLVDILYQRGM KVEK
Sbjct: 601  CCRASWTCVKQFCEIF-DKSRPSDNEFSENSVLIMFNEVIMRSAFLVDILYQRGMCKVEK 660

Query: 661  AVTEILKNWSMAEKLFNGLPVPVQLVKQWVKMQCKHHKNADPRKTTPTLNCLLQSSGMLS 720
            A+TEIL NWS A KLF+ LP PVQLVKQWVKMQCK+HKNADP+K+TPTLNCLLQSS  +S
Sbjct: 661  AMTEILTNWSAAAKLFDELPAPVQLVKQWVKMQCKYHKNADPKKSTPTLNCLLQSSETVS 720

Query: 721  KAQIGALLEQELVEYAEMSGLYPEFCQSLQMKILSTLLRDVYVTPDHRLEKARVLLKKAR 780
            KA+IG LLEQELVEY EMSGLYPEFCQSLQMKILS LL D Y+TPDHRLEKARVL+KKAR
Sbjct: 721  KAKIGVLLEQELVEYEEMSGLYPEFCQSLQMKILSILLTDTYITPDHRLEKARVLMKKAR 780

Query: 781  VLRFCETICLDNCIQCLSEAISTINETSGGTCSSGVLHSHQLAVAYCLRALCTHETVPNS 840
            VLR CET+CLD CIQCLSEAIST+NE SGG+ S GVLHSHQLAVAYCLRA CTHETVPNS
Sbjct: 781  VLRLCETVCLDGCIQCLSEAISTMNEISGGSRSFGVLHSHQLAVAYCLRAFCTHETVPNS 840

Query: 841  KQVLQDIDSALSVWLEISSLDSLPDDQQLILSEYMLLLLCNTFDLLSIKGCMDYYHNIYS 900
            KQV QDIDSALS+WLEISSL++LPDDQ+LILSEYMLLLLCN FDLLSIKGC+DYY NIYS
Sbjct: 841  KQVQQDIDSALSIWLEISSLNTLPDDQRLILSEYMLLLLCNAFDLLSIKGCVDYYDNIYS 900

Query: 901  LMIRLFKWKNVQLEKLLAVLWEGRRMSHAICIAPANEIVITQLSEHLGELPKSFNFWTNC 960
            LMIRLFKWKNV LEKL+A LWE RRMSHA+CIAPANE+VI QLSEHLG+LPKS++FWTNC
Sbjct: 901  LMIRLFKWKNVPLEKLMATLWESRRMSHAMCIAPANELVIAQLSEHLGDLPKSYDFWTNC 960

Query: 961  LKTLPGMLVGFRQNFSFLCRNNGQSCCEHEKSVQLDVTVDEVKEAASKLISHTPACKSSI 1020
            LKTLPGMLVGF+QNFSFLC N  Q+ CEHEKS ++ VTVDEVKEAA KLISH PACKSSI
Sbjct: 961  LKTLPGMLVGFQQNFSFLCSNYTQTSCEHEKSSRMHVTVDEVKEAALKLISHIPACKSSI 1020

Query: 1021 FLAGYLYYDLCERLISEGCLTEALSCAKEAHRLRSKLFQEKFTYSIEQHPEKYCEISHVS 1080
            F AGYLYYDLCERLISEGCLTEAL CAKEAHRLRSKLFQEKFTYS+EQHPEK+CEI+H S
Sbjct: 1021 FSAGYLYYDLCERLISEGCLTEALLCAKEAHRLRSKLFQEKFTYSVEQHPEKFCEITHAS 1080

Query: 1081 QKPAYGIKNLQKNGLVARDVWSFDIISWDVEGCYLSPFNVLQCYLESTLQVGLVHEMIGN 1140
            QKP YGIKNL+K+G V+R++WSFD ISWDVEGCYLSPFNVLQCYLESTLQVGLVHE+IGN
Sbjct: 1081 QKPPYGIKNLRKSGSVSRNIWSFDKISWDVEGCYLSPFNVLQCYLESTLQVGLVHEIIGN 1140

Query: 1141 GSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLARKELEGAKEILTDSIT 1200
            GSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLA+KELEGAK+ILTD+IT
Sbjct: 1141 GSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDNIT 1200

Query: 1201 SCLKCKLVLEVIVDQHLGDLFRSMYVNANGSISEGQLENAEELYKLALEKLNLSAWKNFI 1260
            SCLKC+LVLEVIVDQ+LGDLFRSMYVN NG+ SE  L NAEELYKLALEKLNLS WKN I
Sbjct: 1201 SCLKCRLVLEVIVDQYLGDLFRSMYVNGNGNTSEELLINAEELYKLALEKLNLSGWKNSI 1260

Query: 1261 SFPDEERYLSSLTIQAERPKARKDGRKCKKTTNASRPFQSDQCVNSQSNVRLTRSRCRTI 1320
            S PDEE +LSSLTI  ER KARKDGRK KKTTNA R  Q DQCVN Q+NVRLTRSRCR+I
Sbjct: 1261 SCPDEESHLSSLTIPVERAKARKDGRKSKKTTNAPRSLQMDQCVNPQTNVRLTRSRCRSI 1320

Query: 1321 QGQSTSNSNEVEVDLSVHLKSNIPDLSSASCQKQSYLQVNSCTPTFGCGASCKNGKVGCW 1380
            QGQST+ SNEVEVDL+VHLKSN+PDLS AS QKQ +LQVN CTPT GCGASCKNGKVGCW
Sbjct: 1321 QGQSTNISNEVEVDLTVHLKSNVPDLSGASGQKQMHLQVNCCTPTLGCGASCKNGKVGCW 1380

Query: 1381 QCLPTEIMEAGEMNNFIYLKWEFVRRRLLLRHLSGLGKCSEIRGQIHQTHETILKSMSIL 1440
            QCL  EIMEAG++NNFIYLKWEF RRRLLLR LSGLGKCS IRGQIHQTHETILKSMSIL
Sbjct: 1381 QCLQMEIMEAGQINNFIYLKWEFARRRLLLRQLSGLGKCSGIRGQIHQTHETILKSMSIL 1440

Query: 1441 VSRNLFFQAHYAVEPMILLELVGKEVHRDMFAVERASVLYNICCFSLKSYKYDDSKITCC 1500
            VSRNLF QAH  VEP++LL+LVGKEV  DMFAVERASVLYNIC F+LKSYKYDD+KI CC
Sbjct: 1441 VSRNLFSQAHGVVEPVVLLDLVGKEVPGDMFAVERASVLYNICWFALKSYKYDDTKIICC 1500

Query: 1501 PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSSSELFSLPSSNKILTDS 1560
            PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSS EL SLPSSNKILTDS
Sbjct: 1501 PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSSIELLSLPSSNKILTDS 1560

Query: 1561 HWASYFHQASIGTHLNYQFFPYMTRRSCVEDLNFAKVLWNLLFFFVGFCRFLCCSSYNWI 1620
            HWASYFHQASIGTHLN+QFFPY T RSC++DLNFAK                        
Sbjct: 1561 HWASYFHQASIGTHLNHQFFPYTTGRSCIQDLNFAK------------------------ 1620

Query: 1621 VAYVIFWIFLFVYFSLYLFSFFFLLIVDACILHLKYGLIDGFNKVYFQGFDAGEQTLKLF 1680
                                                            GFD GEQT+KLF
Sbjct: 1621 ------------------------------------------------GFDTGEQTVKLF 1680

Query: 1681 RKGPFSSQDLEEHLRKFFDGLPCVTMVCISLIGGDLACLLQQILHFPSSVHAWILVSHLS 1740
            R G FSSQDLEE++RKFFDGLPCV MVCISLIGGDLACLLQQILHFPSSVHAWILVSHL+
Sbjct: 1681 R-GSFSSQDLEEYVRKFFDGLPCVAMVCISLIGGDLACLLQQILHFPSSVHAWILVSHLN 1740

Query: 1741 SKRHPLVLLLPVETILKEGSEDYSNLQSDDSCERNDLAKHWQCPWGSSVIDEIAPAFRII 1800
            SKR PLVLLLPVETILKE SE YSN QSDD CERND  KHWQCPWGSSVIDEIAP+FR I
Sbjct: 1741 SKRQPLVLLLPVETILKEDSEAYSNPQSDDICERNDSTKHWQCPWGSSVIDEIAPSFRTI 1800

Query: 1801 LEENYLSSSVFPLEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYMLLGDWSTR 1860
            LEENYLSSSVFP EDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKY+LLG WS R
Sbjct: 1801 LEENYLSSSVFPSEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYILLGGWSNR 1860

Query: 1861 EHFDSVLNTLVLNLKSKCKMDVNESLLKVILEGSEEVLEAFDSKLCSRKGCFIGKTRFYD 1920
            +H DSVLNTLVLNLKSKCKMDVNE LLK+ILEGSE+VL  FDSKL  RKGCF+G+ RFYD
Sbjct: 1861 KHVDSVLNTLVLNLKSKCKMDVNEGLLKIILEGSEDVLVGFDSKLYWRKGCFVGRARFYD 1920

Query: 1921 KERSNPFQNTLNGVDQLSALALKLIKDAKKELEGEENTCREPIILVLDYDVQMLPWENIP 1980
            KE+SNPFQN LNGVD+LS LALKLI+DAKKELEGE+ T REPIILVLDYDVQMLPWENIP
Sbjct: 1921 KEKSNPFQNALNGVDKLSTLALKLIQDAKKELEGEDGTSREPIILVLDYDVQMLPWENIP 1980

Query: 1981 ILQNQEVYRMPSVGCICATLDGRSHQQEQDGGIIAAFPSIDPLDAFYLLNPSGDLNSTQI 2040
            IL+NQEVYRMPSVG IC TLDGR HQQEQD GIIA FPSIDPLDAFYLLNPSGDL+STQI
Sbjct: 1981 ILKNQEVYRMPSVGSICVTLDGRLHQQEQDSGIIATFPSIDPLDAFYLLNPSGDLSSTQI 2040

Query: 2041 EFENWFKDQNLEGKAGYAPTSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS 2100
            EFENWFKDQNLEGKAGYAP SSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS
Sbjct: 2041 EFENWFKDQNLEGKAGYAPKSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS 2100

Query: 2101 LLMGCSSGSLTLNGHYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER 2160
            LLMGCSSGSLTLNG+YVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER
Sbjct: 2101 LLMGCSSGSLTLNGYYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER 2160

Query: 2161 SGALPSSAQCDIVTKEFEAMKISSKHANKKVASKSLPAACESESSSRGHSAHSRMIGSFL 2220
            S +LPSS QCDIVTKE EAMKISSK ANKKVASKSLP ACES +SSR HS HSRMIGSFL
Sbjct: 2161 SCSLPSSVQCDIVTKELEAMKISSKRANKKVASKSLPTACES-TSSRDHSVHSRMIGSFL 2173

Query: 2221 CEAREACNLRYLIGASPVCYGVPTSIRKKKDLT 2253
            CEAREACNLRYLIGASPVCYGVPTSIRKKK+LT
Sbjct: 2221 CEAREACNLRYLIGASPVCYGVPTSIRKKKELT 2173

BLAST of Lcy11g005290 vs. NCBI nr
Match: XP_023533745.1 (separase isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 3751.4 bits (9727), Expect = 0.0e+00
Identity = 1890/2253 (83.89%), Postives = 2003/2253 (88.90%), Query Frame = 0

Query: 1    MASPSESSLISILETADSKGISSLVSDFLQPFSDVKNPKKCKKSAKSTDDSSTIRSLAKK 60
            MASPSE+S+ISILETADSKGI S VSDFL+PFSD+KNPKK KKSAK +DDSS IRSLAKK
Sbjct: 1    MASPSEASIISILETADSKGIFSTVSDFLRPFSDIKNPKKSKKSAKPSDDSSAIRSLAKK 60

Query: 61   FLAFLNRALSILPKRLSEPSKLGNDLELALEFFEVYKLCLGCLESLTSQLSCKPYTVDVQ 120
            FL FLNRALSILPKRLSEPSKLGNDLELALEFFE+YKLCL CLESLTSQLSCKPYTVDVQ
Sbjct: 61   FLTFLNRALSILPKRLSEPSKLGNDLELALEFFEIYKLCLACLESLTSQLSCKPYTVDVQ 120

Query: 121  RVRMVHCMEDWGLFKDAEAEGFGILERLREIDRKSKAGKLDCRVIHDGDKGGGDKDFCLL 180
            RVRMVHCMEDWGLFKDAEAEGF ILERLR IDR S     DCRVIHD DKGGGDKDFCLL
Sbjct: 121  RVRMVHCMEDWGLFKDAEAEGFRILERLRVIDRTS-----DCRVIHDRDKGGGDKDFCLL 180

Query: 181  FAEVVVTIVKCTASGRSKESGDYRRVLGLVEEVRPWFRLLDAKVSEKTQRALVTYLGKCT 240
            FAEVVVT+VKC ASGRSKESGDYRR+LGLVEEVRPWFRLLDAKVSEKTQRAL TYLGKCT
Sbjct: 181  FAEVVVTLVKCAASGRSKESGDYRRLLGLVEEVRPWFRLLDAKVSEKTQRALATYLGKCT 240

Query: 241  IFLVEEVVCFGESLVSLFCRTTFAEYAKSSMRDQIYKLARRICSTLFSLQQEQHTSMLIM 300
            IFLVEE+  FGESLVSLFCR TFAEYAKSS++DQIYKLARRICSTLFSLQQEQH+SMLIM
Sbjct: 241  IFLVEELDYFGESLVSLFCRITFAEYAKSSLKDQIYKLARRICSTLFSLQQEQHSSMLIM 300

Query: 301  KILACVLKSITLEWKDETECTVIEFLQLICYCANKCQSESPECCCTFARHLEEMAADFHQ 360
             IL CVLKS+TLEWKDETE  V+EFLQLICY ANKC S SPECCC FA+HLEEMA +FHQ
Sbjct: 301  HILICVLKSLTLEWKDETELEVVEFLQLICYSANKCLSASPECCCAFAKHLEEMAGEFHQ 360

Query: 361  ATTPFGMNLRLYAAGLKIFSKVPRGETHGSAFSILVDDEDTMQSLVNLNGFLGSYFCIGC 420
            AT P GMNLRLYAAGLKI SK+PRGET GSAFS L+DDEDT+QSLVNLNGFLGSYFCIGC
Sbjct: 361  ATPPLGMNLRLYAAGLKIVSKLPRGETCGSAFSTLLDDEDTIQSLVNLNGFLGSYFCIGC 420

Query: 421  RKGNESCSNEQKDFVDQPCSHGKSNHECDVP-LLSWREAYLFSYLDAIKFLCQPLAESVN 480
            R+GNESCS EQKDFV QPC H  SNHE  VP LLS REAYL SYLDAIKF CQPLAESVN
Sbjct: 421  REGNESCSIEQKDFVGQPCLHRNSNHENGVPSLLSLREAYLSSYLDAIKFFCQPLAESVN 480

Query: 481  SERKEILAEDKAASVLYSIQNTLYQSCDVFLFCQRRAYDAKSDGCDENVNMLLSVVMAAF 540
            +ERKEILAEDKA  VLY+IQNTL+Q CDVFLFCQRRAYDAKSDGCDENVNMLLS+V+AAF
Sbjct: 481  AERKEILAEDKAFPVLYNIQNTLHQFCDVFLFCQRRAYDAKSDGCDENVNMLLSIVVAAF 540

Query: 541  TLSIRTRLEMKRSTDLIKAVISSKWVQPFALKHLFASLNNIGIILYRNKQIREASKALKL 600
            TLS RTRLEMKRSTDLIK VISSKWVQPFALK LFASLNNIGIIL+RNKQI EASKALKL
Sbjct: 541  TLSFRTRLEMKRSTDLIKDVISSKWVQPFALKRLFASLNNIGIILFRNKQIGEASKALKL 600

Query: 601  CCRASWTCVRHFCVMFDDKAKPSDNEFSENAVLSFVNEVIMRSAFLVDILYQRGMHKVEK 660
            CCRASWTCV+ FC +F DK++PSDNEFSEN+VL   NEVIMRSAFLVDILYQRGM KVEK
Sbjct: 601  CCRASWTCVKQFCEIF-DKSRPSDNEFSENSVLIMFNEVIMRSAFLVDILYQRGMCKVEK 660

Query: 661  AVTEILKNWSMAEKLFNGLPVPVQLVKQWVKMQCKHHKNADPRKTTPTLNCLLQSSGMLS 720
            A+TEIL NWS A KLF+ LP PVQLVKQWVKMQCK+HKNADP+K+TPTLNCLLQSS  +S
Sbjct: 661  AMTEILTNWSAAAKLFDELPAPVQLVKQWVKMQCKYHKNADPKKSTPTLNCLLQSSETVS 720

Query: 721  KAQIGALLEQELVEYAEMSGLYPEFCQSLQMKILSTLLRDVYVTPDHRLEKARVLLKKAR 780
            KA+IG LLEQELVEY EMSGLYPEFCQSLQMKILS LL D Y+TPDHRLEKARVL+KKAR
Sbjct: 721  KAKIGVLLEQELVEYEEMSGLYPEFCQSLQMKILSILLTDTYITPDHRLEKARVLMKKAR 780

Query: 781  VLRFCETICLDNCIQCLSEAISTINETSGGTCSSGVLHSHQLAVAYCLRALCTHETVPNS 840
            VLR CET+CLD CIQCLSEAIST+NE SGG+ S GVLHSHQLAVAYCLRA CTHETVPNS
Sbjct: 781  VLRLCETVCLDGCIQCLSEAISTMNEISGGSRSFGVLHSHQLAVAYCLRAFCTHETVPNS 840

Query: 841  KQVLQDIDSALSVWLEISSLDSLPDDQQLILSEYMLLLLCNTFDLLSIKGCMDYYHNIYS 900
            KQV QDIDSALS+WLEISSL++LPDDQ+LILSEYMLLLLCN FDLLSIKGC+DYY NIYS
Sbjct: 841  KQVQQDIDSALSIWLEISSLNTLPDDQRLILSEYMLLLLCNAFDLLSIKGCVDYYDNIYS 900

Query: 901  LMIRLFKWKNVQLEKLLAVLWEGRRMSHAICIAPANEIVITQLSEHLGELPKSFNFWTNC 960
            LMIRLFKWKNV LEKL+A LWE RRMSHA+CIAPANE+VI QLSEHLG+LPKS++FWTNC
Sbjct: 901  LMIRLFKWKNVPLEKLMATLWESRRMSHAMCIAPANELVIAQLSEHLGDLPKSYDFWTNC 960

Query: 961  LKTLPGMLVGFRQNFSFLCRNNGQSCCEHEKSVQLDVTVDEVKEAASKLISHTPACKSSI 1020
            LKTLPGMLVGF+QNFSFLC N  Q+ CEHEKS ++ VTVDEVKEAA KLISH PACKSSI
Sbjct: 961  LKTLPGMLVGFQQNFSFLCSNYTQTSCEHEKSSRMHVTVDEVKEAALKLISHIPACKSSI 1020

Query: 1021 FLAGYLYYDLCERLISEGCLTEALSCAKEAHRLRSKLFQEKFTYSIEQHPEKYCEISHVS 1080
            F AGYLYYDLCERLISEGCLTEAL CAKEAHRLRSKLFQEKFTYS+EQHPEK+CEI+H S
Sbjct: 1021 FSAGYLYYDLCERLISEGCLTEALLCAKEAHRLRSKLFQEKFTYSVEQHPEKFCEITHAS 1080

Query: 1081 QKPAYGIKNLQKNGLVARDVWSFDIISWDVEGCYLSPFNVLQCYLESTLQVGLVHEMIGN 1140
            QKP YGIKNL+K+G V+R++WSFD ISWDVEGCYLSPFNVLQCYLESTLQVGLVHE+IGN
Sbjct: 1081 QKPPYGIKNLRKSGSVSRNIWSFDKISWDVEGCYLSPFNVLQCYLESTLQVGLVHEIIGN 1140

Query: 1141 GSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLARKELEGAKEILTDSIT 1200
            GSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLA+KELEGAK+ILTD+IT
Sbjct: 1141 GSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDNIT 1200

Query: 1201 SCLKCKLVLEVIVDQHLGDLFRSMYVNANGSISEGQLENAEELYKLALEKLNLSAWKNFI 1260
            SCLKC+LVLEVIVDQ+LGDLFRSMYVN NG+ SE  L NAEELYKLALEKLNLS WKN I
Sbjct: 1201 SCLKCRLVLEVIVDQYLGDLFRSMYVNGNGNTSEELLINAEELYKLALEKLNLSGWKNSI 1260

Query: 1261 SFPDEERYLSSLTIQAERPKARKDGRKCKKTTNASRPFQSDQCVNSQSNVRLTRSRCRTI 1320
            S PDEE +LSSLTI  ER KARKDGRK KKTTNA R  Q DQCVN Q+NVRLTRSRCR+I
Sbjct: 1261 SCPDEESHLSSLTIPVERAKARKDGRKSKKTTNAPRSLQMDQCVNPQTNVRLTRSRCRSI 1320

Query: 1321 QGQSTSNSNEVEVDLSVHLKSNIPDLSSASCQKQSYLQVNSCTPTFGCGASCKNGKVGCW 1380
            QGQST+ SNEVEVDL+VHLKSN+PDLS AS QKQ +LQVN CTPT GCGASCKNGKVGCW
Sbjct: 1321 QGQSTNISNEVEVDLTVHLKSNVPDLSGASGQKQMHLQVNCCTPTLGCGASCKNGKVGCW 1380

Query: 1381 QCLPTEIMEAGEMNNFIYLKWEFVRRRLLLRHLSGLGKCSEIRGQIHQTHETILKSMSIL 1440
            QCL  EIMEAG++NNFIYLKWEF RRRLLLR LSGLGKCS IRGQIHQTHETILKSMSIL
Sbjct: 1381 QCLQMEIMEAGQINNFIYLKWEFARRRLLLRQLSGLGKCSGIRGQIHQTHETILKSMSIL 1440

Query: 1441 VSRNLFFQAHYAVEPMILLELVGKEVHRDMFAVERASVLYNICCFSLKSYKYDDSKITCC 1500
            VSRNLF QAH  VEP++LL+LVGKEV  DMFAVERASVLYNIC F+LKSYKYDD+KI CC
Sbjct: 1441 VSRNLFSQAHGVVEPVVLLDLVGKEVPGDMFAVERASVLYNICWFALKSYKYDDTKIICC 1500

Query: 1501 PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSSSELFSLPSSNKILTDS 1560
            PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSS EL SLPSSNKILTDS
Sbjct: 1501 PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSSIELLSLPSSNKILTDS 1560

Query: 1561 HWASYFHQASIGTHLNYQFFPYMTRRSCVEDLNFAKVLWNLLFFFVGFCRFLCCSSYNWI 1620
            HWASYFHQASIGTHLN+QFFPY T RSC++DLNFAK                        
Sbjct: 1561 HWASYFHQASIGTHLNHQFFPYTTGRSCIQDLNFAK------------------------ 1620

Query: 1621 VAYVIFWIFLFVYFSLYLFSFFFLLIVDACILHLKYGLIDGFNKVYFQGFDAGEQTLKLF 1680
                                                            GFD GEQT+KLF
Sbjct: 1621 ------------------------------------------------GFDTGEQTVKLF 1680

Query: 1681 RKGPFSSQDLEEHLRKFFDGLPCVTMVCISLIGGDLACLLQQILHFPSSVHAWILVSHLS 1740
            R+G FSSQDLEE++RKFFDGLPCV MVCISLIGGDLACLLQQILHFPSSVHAWILVSHL+
Sbjct: 1681 RRGSFSSQDLEEYVRKFFDGLPCVAMVCISLIGGDLACLLQQILHFPSSVHAWILVSHLN 1740

Query: 1741 SKRHPLVLLLPVETILKEGSEDYSNLQSDDSCERNDLAKHWQCPWGSSVIDEIAPAFRII 1800
            SKR PLVLLLPVETILK+ SE YSN QSDD CERND  KHWQCPWGSSVIDEIAP+FR I
Sbjct: 1741 SKRQPLVLLLPVETILKD-SEAYSNPQSDDICERNDSTKHWQCPWGSSVIDEIAPSFRTI 1800

Query: 1801 LEENYLSSSVFPLEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYMLLGDWSTR 1860
            LEENYLSSSVFP EDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKY+LLG WS R
Sbjct: 1801 LEENYLSSSVFPSEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYILLGGWSNR 1860

Query: 1861 EHFDSVLNTLVLNLKSKCKMDVNESLLKVILEGSEEVLEAFDSKLCSRKGCFIGKTRFYD 1920
            +H DSVLNTLVLNLKSKCKMDVNE LLK+ILEGSE+VL  FDSKL  RKGCF+G+ RFYD
Sbjct: 1861 KHVDSVLNTLVLNLKSKCKMDVNEGLLKIILEGSEDVLVGFDSKLYWRKGCFVGRARFYD 1920

Query: 1921 KERSNPFQNTLNGVDQLSALALKLIKDAKKELEGEENTCREPIILVLDYDVQMLPWENIP 1980
            KE+SNPFQN LNGVD+LS LALKLI+DAKKELEGE+ T REPIILVLDYDVQMLPWENIP
Sbjct: 1921 KEKSNPFQNALNGVDKLSTLALKLIQDAKKELEGEDGTSREPIILVLDYDVQMLPWENIP 1980

Query: 1981 ILQNQEVYRMPSVGCICATLDGRSHQQEQDGGIIAAFPSIDPLDAFYLLNPSGDLNSTQI 2040
            IL+NQEVYRMPSVG IC TLDGR HQQEQD GIIA FPSIDPLDAFYLLNPSGDL+STQI
Sbjct: 1981 ILKNQEVYRMPSVGSICVTLDGRLHQQEQDSGIIATFPSIDPLDAFYLLNPSGDLSSTQI 2040

Query: 2041 EFENWFKDQNLEGKAGYAPTSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS 2100
            EFENWFKDQNLEGKAGYAP SSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS
Sbjct: 2041 EFENWFKDQNLEGKAGYAPKSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS 2100

Query: 2101 LLMGCSSGSLTLNGHYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER 2160
            LLMGCSSGSLTLNG+YVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER
Sbjct: 2101 LLMGCSSGSLTLNGYYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER 2160

Query: 2161 SGALPSSAQCDIVTKEFEAMKISSKHANKKVASKSLPAACESESSSRGHSAHSRMIGSFL 2220
            S +LPSS QCDIVTKE EAMKISSK ANKKVASKSLP ACES +SSR HS HSRMIGSFL
Sbjct: 2161 SCSLPSSVQCDIVTKELEAMKISSKRANKKVASKSLPTACES-TSSRDHSVHSRMIGSFL 2173

Query: 2221 CEAREACNLRYLIGASPVCYGVPTSIRKKKDLT 2253
            CEAREACNLRYLIGASPVCYGVPTSIRKKK+LT
Sbjct: 2221 CEAREACNLRYLIGASPVCYGVPTSIRKKKELT 2173

BLAST of Lcy11g005290 vs. NCBI nr
Match: XP_022942950.1 (separase isoform X1 [Cucurbita moschata])

HSP 1 Score: 3745.7 bits (9712), Expect = 0.0e+00
Identity = 1884/2253 (83.62%), Postives = 2003/2253 (88.90%), Query Frame = 0

Query: 1    MASPSESSLISILETADSKGISSLVSDFLQPFSDVKNPKKCKKSAKSTDDSSTIRSLAKK 60
            MASPSE+S+ISILETADSKGI S VSDFL+PFSD+KNPKK KKSAK +DDSS IRSLAKK
Sbjct: 1    MASPSEASIISILETADSKGIFSTVSDFLRPFSDIKNPKKSKKSAKPSDDSSAIRSLAKK 60

Query: 61   FLAFLNRALSILPKRLSEPSKLGNDLELALEFFEVYKLCLGCLESLTSQLSCKPYTVDVQ 120
            FLAFLNRALSILPKRLSEPSKLGNDLELALEFFE+Y+LCL CLESLTSQLSCKPYTVDVQ
Sbjct: 61   FLAFLNRALSILPKRLSEPSKLGNDLELALEFFEIYRLCLACLESLTSQLSCKPYTVDVQ 120

Query: 121  RVRMVHCMEDWGLFKDAEAEGFGILERLREIDRKSKAGKLDCRVIHDGDKGGGDKDFCLL 180
            RVRMVHCMEDWGLFKDAEAEGF ILERLR IDR S     DCRVIHD DKGGGDK+FCLL
Sbjct: 121  RVRMVHCMEDWGLFKDAEAEGFRILERLRVIDRTS-----DCRVIHDRDKGGGDKEFCLL 180

Query: 181  FAEVVVTIVKCTASGRSKESGDYRRVLGLVEEVRPWFRLLDAKVSEKTQRALVTYLGKCT 240
            FAEVVVT+VKC ASGRSKESGDY R+LGLVEEVRPWFRLLDAKVSEKTQRAL TYLGKCT
Sbjct: 181  FAEVVVTLVKCAASGRSKESGDYGRLLGLVEEVRPWFRLLDAKVSEKTQRALATYLGKCT 240

Query: 241  IFLVEEVVCFGESLVSLFCRTTFAEYAKSSMRDQIYKLARRICSTLFSLQQEQHTSMLIM 300
            IFLVEE+  FGESLVSLFCR TFAEYAKSS++DQIYKLARRICSTLFSLQQEQH+SMLIM
Sbjct: 241  IFLVEELDYFGESLVSLFCRITFAEYAKSSLKDQIYKLARRICSTLFSLQQEQHSSMLIM 300

Query: 301  KILACVLKSITLEWKDETECTVIEFLQLICYCANKCQSESPECCCTFARHLEEMAADFHQ 360
             IL CV+KS+TLEWKDETE  V+EFLQLICY ANKC S SPECCC FA+HL+EMA +FHQ
Sbjct: 301  HILICVVKSLTLEWKDETEWEVVEFLQLICYSANKCLSTSPECCCAFAKHLQEMAGEFHQ 360

Query: 361  ATTPFGMNLRLYAAGLKIFSKVPRGETHGSAFSILVDDEDTMQSLVNLNGFLGSYFCIGC 420
            AT P GMNLRLYAAGLKI SK+PRGET GSAFS L+DDEDT+QSLVNLNGFLGSYFCIGC
Sbjct: 361  ATPPLGMNLRLYAAGLKIVSKLPRGETCGSAFSTLLDDEDTIQSLVNLNGFLGSYFCIGC 420

Query: 421  RKGNESCSNEQKDFVDQPCSHGKSNHECDVP-LLSWREAYLFSYLDAIKFLCQPLAESVN 480
            R+GNESCS EQKDFV QPC H  SNHE  VP LLS REAYL SYLDAIKF CQPLAESVN
Sbjct: 421  REGNESCSIEQKDFVGQPCLHRNSNHENGVPSLLSLREAYLSSYLDAIKFFCQPLAESVN 480

Query: 481  SERKEILAEDKAASVLYSIQNTLYQSCDVFLFCQRRAYDAKSDGCDENVNMLLSVVMAAF 540
             ERKEILAEDKAA VLY+IQNTL+Q CDVFLFCQRRAYDAKSDGCDENVNMLLS+V+AAF
Sbjct: 481  VERKEILAEDKAAPVLYNIQNTLHQFCDVFLFCQRRAYDAKSDGCDENVNMLLSIVVAAF 540

Query: 541  TLSIRTRLEMKRSTDLIKAVISSKWVQPFALKHLFASLNNIGIILYRNKQIREASKALKL 600
            TLS RTRL+MKRST+LIK VI+SKWVQPFALK LFASLNNIGIIL+RNKQI EASKALKL
Sbjct: 541  TLSFRTRLDMKRSTNLIKDVIASKWVQPFALKRLFASLNNIGIILFRNKQIGEASKALKL 600

Query: 601  CCRASWTCVRHFCVMFDDKAKPSDNEFSENAVLSFVNEVIMRSAFLVDILYQRGMHKVEK 660
            CCRASWTCV+ FC +FD+K++PSDNEFSEN+VL  VNEVIMRSAFLVDILYQ  M KVEK
Sbjct: 601  CCRASWTCVKQFCEIFDNKSRPSDNEFSENSVLIIVNEVIMRSAFLVDILYQCDMCKVEK 660

Query: 661  AVTEILKNWSMAEKLFNGLPVPVQLVKQWVKMQCKHHKNADPRKTTPTLNCLLQSSGMLS 720
            A+TEIL NWS A KLF+ LPVPVQLVKQWVKMQCK+HKNADP+K+TPTLNCLLQSS  +S
Sbjct: 661  AMTEILTNWSAAAKLFDELPVPVQLVKQWVKMQCKYHKNADPKKSTPTLNCLLQSSETVS 720

Query: 721  KAQIGALLEQELVEYAEMSGLYPEFCQSLQMKILSTLLRDVYVTPDHRLEKARVLLKKAR 780
            KA+IG LLEQELVEY EMSGLYPEFCQSLQMKILS LL D Y+TPDHRLEKARVL+KKAR
Sbjct: 721  KARIGVLLEQELVEYEEMSGLYPEFCQSLQMKILSILLTDTYITPDHRLEKARVLMKKAR 780

Query: 781  VLRFCETICLDNCIQCLSEAISTINETSGGTCSSGVLHSHQLAVAYCLRALCTHETVPNS 840
            VLR CET+CLD CIQCLSEAIST+NE S G+ S GVLHSHQLAVAYCLRA CTHETVPNS
Sbjct: 781  VLRLCETVCLDGCIQCLSEAISTMNEISSGSRSFGVLHSHQLAVAYCLRAFCTHETVPNS 840

Query: 841  KQVLQDIDSALSVWLEISSLDSLPDDQQLILSEYMLLLLCNTFDLLSIKGCMDYYHNIYS 900
            KQV QDIDSALS+WLEISSL++LPDDQ+LILSEYMLLLLCN FDLLSIKGC+DY+ NIYS
Sbjct: 841  KQVQQDIDSALSIWLEISSLNTLPDDQRLILSEYMLLLLCNAFDLLSIKGCVDYHDNIYS 900

Query: 901  LMIRLFKWKNVQLEKLLAVLWEGRRMSHAICIAPANEIVITQLSEHLGELPKSFNFWTNC 960
            LMIRLFKWKNV LEKL+A LWE RRMSHA+CIAPANEIVI QLSEHLG+LPKS +FWTNC
Sbjct: 901  LMIRLFKWKNVPLEKLMATLWESRRMSHAMCIAPANEIVIAQLSEHLGDLPKSNDFWTNC 960

Query: 961  LKTLPGMLVGFRQNFSFLCRNNGQSCCEHEKSVQLDVTVDEVKEAASKLISHTPACKSSI 1020
            LKTLPGMLVGF+QNFSFLC N  Q+ CEHEKS ++ VTVDEVKEAA KLISH PACKSSI
Sbjct: 961  LKTLPGMLVGFQQNFSFLCSNYTQTSCEHEKSSRMHVTVDEVKEAALKLISHIPACKSSI 1020

Query: 1021 FLAGYLYYDLCERLISEGCLTEALSCAKEAHRLRSKLFQEKFTYSIEQHPEKYCEISHVS 1080
            F AGYLYYDLCERLISEGCLTEAL CAKEAHRLRSKLFQEKFTYS+EQHPEKYCEI+H S
Sbjct: 1021 FSAGYLYYDLCERLISEGCLTEALLCAKEAHRLRSKLFQEKFTYSVEQHPEKYCEITHAS 1080

Query: 1081 QKPAYGIKNLQKNGLVARDVWSFDIISWDVEGCYLSPFNVLQCYLESTLQVGLVHEMIGN 1140
            QKP YGIKNL+K+G V+R++WSFD ISWDVEGCYLSPFNVLQCYLESTLQVGLVHE+IGN
Sbjct: 1081 QKPPYGIKNLRKSGSVSRNIWSFDKISWDVEGCYLSPFNVLQCYLESTLQVGLVHEIIGN 1140

Query: 1141 GSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLARKELEGAKEILTDSIT 1200
            GSEAETLLQWGKSISCL+SLPLFEVAFSSALGKVYRKKQLWSLA+KELEGAK+ILTD+IT
Sbjct: 1141 GSEAETLLQWGKSISCLRSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDNIT 1200

Query: 1201 SCLKCKLVLEVIVDQHLGDLFRSMYVNANGSISEGQLENAEELYKLALEKLNLSAWKNFI 1260
            SCLKC+LVLEVIVDQ+LGDLFRSMYVN NG+ SE  L NAEELYKLALEKLNLS WKN I
Sbjct: 1201 SCLKCRLVLEVIVDQYLGDLFRSMYVNGNGNTSEELLINAEELYKLALEKLNLSGWKNSI 1260

Query: 1261 SFPDEERYLSSLTIQAERPKARKDGRKCKKTTNASRPFQSDQCVNSQSNVRLTRSRCRTI 1320
            S PDE+ +LSSLTI  ER KARKDGRK KKTTNA R  + DQCVN Q+NVRLTRSRCR+I
Sbjct: 1261 SCPDEDSHLSSLTIPVERAKARKDGRKSKKTTNAPRSLKMDQCVNPQTNVRLTRSRCRSI 1320

Query: 1321 QGQSTSNSNEVEVDLSVHLKSNIPDLSSASCQKQSYLQVNSCTPTFGCGASCKNGKVGCW 1380
            QGQST+ SNEVEVDL+VHLKSN+PDL  AS QKQ +LQVN CTPT GCGASCKNGKVGCW
Sbjct: 1321 QGQSTNISNEVEVDLTVHLKSNVPDLFGASGQKQMHLQVNCCTPTLGCGASCKNGKVGCW 1380

Query: 1381 QCLPTEIMEAGEMNNFIYLKWEFVRRRLLLRHLSGLGKCSEIRGQIHQTHETILKSMSIL 1440
            QCL  EIMEAG++NNFIYLKWEFVRRRLLLR LSGLGKCS IRGQIHQTHETILKSMSIL
Sbjct: 1381 QCLQMEIMEAGQINNFIYLKWEFVRRRLLLRQLSGLGKCSGIRGQIHQTHETILKSMSIL 1440

Query: 1441 VSRNLFFQAHYAVEPMILLELVGKEVHRDMFAVERASVLYNICCFSLKSYKYDDSKITCC 1500
            VSRN+F QAH  VEP++LL+LVGKEV  DMFAVERASVLYNIC F+LKSYKYDD+KI CC
Sbjct: 1441 VSRNIFSQAHGVVEPVVLLDLVGKEVPGDMFAVERASVLYNICWFALKSYKYDDTKIICC 1500

Query: 1501 PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSSSELFSLPSSNKILTDS 1560
            PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSS EL SLPSSNKILTDS
Sbjct: 1501 PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSSIELLSLPSSNKILTDS 1560

Query: 1561 HWASYFHQASIGTHLNYQFFPYMTRRSCVEDLNFAKVLWNLLFFFVGFCRFLCCSSYNWI 1620
            HWASYFHQASIGTHLN+QFFPY T RSC++DLNFAK                        
Sbjct: 1561 HWASYFHQASIGTHLNHQFFPYTTGRSCIQDLNFAK------------------------ 1620

Query: 1621 VAYVIFWIFLFVYFSLYLFSFFFLLIVDACILHLKYGLIDGFNKVYFQGFDAGEQTLKLF 1680
                                                            GFD GEQT+KLF
Sbjct: 1621 ------------------------------------------------GFDTGEQTVKLF 1680

Query: 1681 RKGPFSSQDLEEHLRKFFDGLPCVTMVCISLIGGDLACLLQQILHFPSSVHAWILVSHLS 1740
            R+G FSSQDLEE++RKFFDGLPCV MVCISLIGGDLACLLQQILHFPSSVHAWILVSHL+
Sbjct: 1681 RRGSFSSQDLEEYVRKFFDGLPCVAMVCISLIGGDLACLLQQILHFPSSVHAWILVSHLN 1740

Query: 1741 SKRHPLVLLLPVETILKEGSEDYSNLQSDDSCERNDLAKHWQCPWGSSVIDEIAPAFRII 1800
            SKR PLVLLLPVETILKE SEDYSN QSDD CERND  KHWQCPWGSSVIDEIAPAFR I
Sbjct: 1741 SKRQPLVLLLPVETILKEDSEDYSNPQSDDICERNDSTKHWQCPWGSSVIDEIAPAFRTI 1800

Query: 1801 LEENYLSSSVFPLEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYMLLGDWSTR 1860
            LE+NYLSSSVFP EDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKY+LLGDWS R
Sbjct: 1801 LEDNYLSSSVFPSEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYILLGDWSNR 1860

Query: 1861 EHFDSVLNTLVLNLKSKCKMDVNESLLKVILEGSEEVLEAFDSKLCSRKGCFIGKTRFYD 1920
            +H DSVLNTLVLNLKSKCKMDVNE LLK+ILEGSE VL  FDSKL SRKGCF+G+ RF+D
Sbjct: 1861 KHVDSVLNTLVLNLKSKCKMDVNEGLLKIILEGSENVLVGFDSKLYSRKGCFVGRARFHD 1920

Query: 1921 KERSNPFQNTLNGVDQLSALALKLIKDAKKELEGEENTCREPIILVLDYDVQMLPWENIP 1980
            KERSNPFQN LNGVD+LS LALKLI+DAKKELEGE+ T REPIILVLDYDVQMLPWENIP
Sbjct: 1921 KERSNPFQNALNGVDKLSTLALKLIQDAKKELEGEDGTSREPIILVLDYDVQMLPWENIP 1980

Query: 1981 ILQNQEVYRMPSVGCICATLDGRSHQQEQDGGIIAAFPSIDPLDAFYLLNPSGDLNSTQI 2040
            IL+NQEVYRMPSVG IC TLDGR HQQEQD GIIA FPSIDPLDAFYLLNPSGDL+STQI
Sbjct: 1981 ILKNQEVYRMPSVGSICVTLDGRLHQQEQDSGIIATFPSIDPLDAFYLLNPSGDLSSTQI 2040

Query: 2041 EFENWFKDQNLEGKAGYAPTSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS 2100
            EFENWFKDQNLEGKAGYAP SSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS
Sbjct: 2041 EFENWFKDQNLEGKAGYAPKSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS 2100

Query: 2101 LLMGCSSGSLTLNGHYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER 2160
            LLMGCSSGSLTLNG+YVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER
Sbjct: 2101 LLMGCSSGSLTLNGYYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER 2160

Query: 2161 SGALPSSAQCDIVTKEFEAMKISSKHANKKVASKSLPAACESESSSRGHSAHSRMIGSFL 2220
            S ALPSS QCDIVTKE EAMKISSK ANKKVASKSLP  CES SSS+ HS HSRMIGSFL
Sbjct: 2161 SCALPSSVQCDIVTKELEAMKISSKLANKKVASKSLPTTCES-SSSKDHSVHSRMIGSFL 2175

Query: 2221 CEAREACNLRYLIGASPVCYGVPTSIRKKKDLT 2253
            CEAREACNLRYLIGASPVCYGVPTSIRKKK+LT
Sbjct: 2221 CEAREACNLRYLIGASPVCYGVPTSIRKKKELT 2175

BLAST of Lcy11g005290 vs. NCBI nr
Match: XP_022942953.1 (separase isoform X3 [Cucurbita moschata])

HSP 1 Score: 3740.3 bits (9698), Expect = 0.0e+00
Identity = 1884/2253 (83.62%), Postives = 2002/2253 (88.86%), Query Frame = 0

Query: 1    MASPSESSLISILETADSKGISSLVSDFLQPFSDVKNPKKCKKSAKSTDDSSTIRSLAKK 60
            MASPSE+S+ISILETADSKGI S VSDFL+PFSD+KNPKK KKSAK +DDSS IRSLAKK
Sbjct: 1    MASPSEASIISILETADSKGIFSTVSDFLRPFSDIKNPKKSKKSAKPSDDSSAIRSLAKK 60

Query: 61   FLAFLNRALSILPKRLSEPSKLGNDLELALEFFEVYKLCLGCLESLTSQLSCKPYTVDVQ 120
            FLAFLNRALSILPKRLSEPSKLGNDLELALEFFE+Y+LCL CLESLTSQLSCKPYTVDVQ
Sbjct: 61   FLAFLNRALSILPKRLSEPSKLGNDLELALEFFEIYRLCLACLESLTSQLSCKPYTVDVQ 120

Query: 121  RVRMVHCMEDWGLFKDAEAEGFGILERLREIDRKSKAGKLDCRVIHDGDKGGGDKDFCLL 180
            RVRMVHCMEDWGLFKDAEAEGF ILERLR IDR S     DCRVIHD DKGGGDK+FCLL
Sbjct: 121  RVRMVHCMEDWGLFKDAEAEGFRILERLRVIDRTS-----DCRVIHDRDKGGGDKEFCLL 180

Query: 181  FAEVVVTIVKCTASGRSKESGDYRRVLGLVEEVRPWFRLLDAKVSEKTQRALVTYLGKCT 240
            FAEVVVT+VKC ASGRSKESGDY R+LGLVEEVRPWFRLLDAKVSEKTQRAL TYLGKCT
Sbjct: 181  FAEVVVTLVKCAASGRSKESGDYGRLLGLVEEVRPWFRLLDAKVSEKTQRALATYLGKCT 240

Query: 241  IFLVEEVVCFGESLVSLFCRTTFAEYAKSSMRDQIYKLARRICSTLFSLQQEQHTSMLIM 300
            IFLVEE+  FGESLVSLFCR TFAEYAKSS++DQIYKLARRICSTLFSLQQEQH+SMLIM
Sbjct: 241  IFLVEELDYFGESLVSLFCRITFAEYAKSSLKDQIYKLARRICSTLFSLQQEQHSSMLIM 300

Query: 301  KILACVLKSITLEWKDETECTVIEFLQLICYCANKCQSESPECCCTFARHLEEMAADFHQ 360
             IL CV+KS+TLEWKDETE  V+EFLQLICY ANKC S SPECCC FA+HL+EMA +FHQ
Sbjct: 301  HILICVVKSLTLEWKDETEWEVVEFLQLICYSANKCLSTSPECCCAFAKHLQEMAGEFHQ 360

Query: 361  ATTPFGMNLRLYAAGLKIFSKVPRGETHGSAFSILVDDEDTMQSLVNLNGFLGSYFCIGC 420
            AT P GMNLRLYAAGLKI SK+PRGET GSAFS L+DDEDT+QSLVNLNGFLGSYFCIGC
Sbjct: 361  ATPPLGMNLRLYAAGLKIVSKLPRGETCGSAFSTLLDDEDTIQSLVNLNGFLGSYFCIGC 420

Query: 421  RKGNESCSNEQKDFVDQPCSHGKSNHECDVP-LLSWREAYLFSYLDAIKFLCQPLAESVN 480
            R+GNESCS EQKDFV QPC H  SNHE  VP LLS REAYL SYLDAIKF CQPLAESVN
Sbjct: 421  REGNESCSIEQKDFVGQPCLHRNSNHENGVPSLLSLREAYLSSYLDAIKFFCQPLAESVN 480

Query: 481  SERKEILAEDKAASVLYSIQNTLYQSCDVFLFCQRRAYDAKSDGCDENVNMLLSVVMAAF 540
             ERKEILAEDKAA VLY+IQNTL+Q CDVFLFCQRRAYDAKSDGCDENVNMLLS+V+AAF
Sbjct: 481  VERKEILAEDKAAPVLYNIQNTLHQFCDVFLFCQRRAYDAKSDGCDENVNMLLSIVVAAF 540

Query: 541  TLSIRTRLEMKRSTDLIKAVISSKWVQPFALKHLFASLNNIGIILYRNKQIREASKALKL 600
            TLS RTRL+MKRST+LIK VI+SKWVQPFALK LFASLNNIGIIL+RNKQI EASKALKL
Sbjct: 541  TLSFRTRLDMKRSTNLIKDVIASKWVQPFALKRLFASLNNIGIILFRNKQIGEASKALKL 600

Query: 601  CCRASWTCVRHFCVMFDDKAKPSDNEFSENAVLSFVNEVIMRSAFLVDILYQRGMHKVEK 660
            CCRASWTCV+ FC +FD+K++PSDNEFSEN+VL  VNEVIMRSAFLVDILYQ  M KVEK
Sbjct: 601  CCRASWTCVKQFCEIFDNKSRPSDNEFSENSVLIIVNEVIMRSAFLVDILYQCDMCKVEK 660

Query: 661  AVTEILKNWSMAEKLFNGLPVPVQLVKQWVKMQCKHHKNADPRKTTPTLNCLLQSSGMLS 720
            A+TEIL NWS A KLF+ LPVPVQLVKQWVKMQCK+HKNADP+K+TPTLNCLLQSS  +S
Sbjct: 661  AMTEILTNWSAAAKLFDELPVPVQLVKQWVKMQCKYHKNADPKKSTPTLNCLLQSSETVS 720

Query: 721  KAQIGALLEQELVEYAEMSGLYPEFCQSLQMKILSTLLRDVYVTPDHRLEKARVLLKKAR 780
            KA+IG LLEQELVEY EMSGLYPEFCQSLQMKILS LL D Y+TPDHRLEKARVL+KKAR
Sbjct: 721  KARIGVLLEQELVEYEEMSGLYPEFCQSLQMKILSILLTDTYITPDHRLEKARVLMKKAR 780

Query: 781  VLRFCETICLDNCIQCLSEAISTINETSGGTCSSGVLHSHQLAVAYCLRALCTHETVPNS 840
            VLR CET+CLD CIQCLSEAIST+NE S G+ S GVLHSHQLAVAYCLRA CTHETVPNS
Sbjct: 781  VLRLCETVCLDGCIQCLSEAISTMNEISSGSRSFGVLHSHQLAVAYCLRAFCTHETVPNS 840

Query: 841  KQVLQDIDSALSVWLEISSLDSLPDDQQLILSEYMLLLLCNTFDLLSIKGCMDYYHNIYS 900
            KQV QDIDSALS+WLEISSL++LPDDQ+LILSEYMLLLLCN FDLLSIKGC+DY+ NIYS
Sbjct: 841  KQVQQDIDSALSIWLEISSLNTLPDDQRLILSEYMLLLLCNAFDLLSIKGCVDYHDNIYS 900

Query: 901  LMIRLFKWKNVQLEKLLAVLWEGRRMSHAICIAPANEIVITQLSEHLGELPKSFNFWTNC 960
            LMIRLFKWKNV LEKL+A LWE RRMSHA+CIAPANEIVI QLSEHLG+LPKS +FWTNC
Sbjct: 901  LMIRLFKWKNVPLEKLMATLWESRRMSHAMCIAPANEIVIAQLSEHLGDLPKSNDFWTNC 960

Query: 961  LKTLPGMLVGFRQNFSFLCRNNGQSCCEHEKSVQLDVTVDEVKEAASKLISHTPACKSSI 1020
            LKTLPGMLVGF+QNFSFLC N  Q+ CEHEKS ++ VTVDEVKEAA KLISH PACKSSI
Sbjct: 961  LKTLPGMLVGFQQNFSFLCSNYTQTSCEHEKSSRMHVTVDEVKEAALKLISHIPACKSSI 1020

Query: 1021 FLAGYLYYDLCERLISEGCLTEALSCAKEAHRLRSKLFQEKFTYSIEQHPEKYCEISHVS 1080
            F AGYLYYDLCERLISEGCLTEAL CAKEAHRLRSKLFQEKFTYS+EQHPEKYCEI+H S
Sbjct: 1021 FSAGYLYYDLCERLISEGCLTEALLCAKEAHRLRSKLFQEKFTYSVEQHPEKYCEITHAS 1080

Query: 1081 QKPAYGIKNLQKNGLVARDVWSFDIISWDVEGCYLSPFNVLQCYLESTLQVGLVHEMIGN 1140
            QKP YGIKNL+K+G V+R++WSFD ISWDVEGCYLSPFNVLQCYLESTLQVGLVHE+IGN
Sbjct: 1081 QKPPYGIKNLRKSGSVSRNIWSFDKISWDVEGCYLSPFNVLQCYLESTLQVGLVHEIIGN 1140

Query: 1141 GSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLARKELEGAKEILTDSIT 1200
            GSEAETLLQWGKSISCL+SLPLFEVAFSSALGKVYRKKQLWSLA+KELEGAK+ILTD+IT
Sbjct: 1141 GSEAETLLQWGKSISCLRSLPLFEVAFSSALGKVYRKKQLWSLAQKELEGAKQILTDNIT 1200

Query: 1201 SCLKCKLVLEVIVDQHLGDLFRSMYVNANGSISEGQLENAEELYKLALEKLNLSAWKNFI 1260
            SCLKC+LVLEVIVDQ+LGDLFRSMYVN NG+ SE  L NAEELYKLALEKLNLS WKN I
Sbjct: 1201 SCLKCRLVLEVIVDQYLGDLFRSMYVNGNGNTSEELLINAEELYKLALEKLNLSGWKNSI 1260

Query: 1261 SFPDEERYLSSLTIQAERPKARKDGRKCKKTTNASRPFQSDQCVNSQSNVRLTRSRCRTI 1320
            S PDE+ +LSSLTI  ER KARKDGRK KKTTNA R  + DQCVN Q+NVRLTRSRCR+I
Sbjct: 1261 SCPDEDSHLSSLTIPVERAKARKDGRKSKKTTNAPRSLKMDQCVNPQTNVRLTRSRCRSI 1320

Query: 1321 QGQSTSNSNEVEVDLSVHLKSNIPDLSSASCQKQSYLQVNSCTPTFGCGASCKNGKVGCW 1380
            QGQST+ SNEVEVDL+VHLKSN+PDL  AS QKQ +LQVN CTPT GCGASCKNGKVGCW
Sbjct: 1321 QGQSTNISNEVEVDLTVHLKSNVPDLFGASGQKQMHLQVNCCTPTLGCGASCKNGKVGCW 1380

Query: 1381 QCLPTEIMEAGEMNNFIYLKWEFVRRRLLLRHLSGLGKCSEIRGQIHQTHETILKSMSIL 1440
            QCL  EIMEAG++NNFIYLKWEFVRRRLLLR LSGLGKCS IRGQIHQTHETILKSMSIL
Sbjct: 1381 QCLQMEIMEAGQINNFIYLKWEFVRRRLLLRQLSGLGKCSGIRGQIHQTHETILKSMSIL 1440

Query: 1441 VSRNLFFQAHYAVEPMILLELVGKEVHRDMFAVERASVLYNICCFSLKSYKYDDSKITCC 1500
            VSRN+F QAH  VEP++LL+LVGKEV  DMFAVERASVLYNIC F+LKSYKYDD+KI CC
Sbjct: 1441 VSRNIFSQAHGVVEPVVLLDLVGKEVPGDMFAVERASVLYNICWFALKSYKYDDTKIICC 1500

Query: 1501 PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSSSELFSLPSSNKILTDS 1560
            PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSS EL SLPSSNKILTDS
Sbjct: 1501 PLSQVQSKTLVSWLMVALVLCCEVPVLFQKVSKLLAVIHVISSSIELLSLPSSNKILTDS 1560

Query: 1561 HWASYFHQASIGTHLNYQFFPYMTRRSCVEDLNFAKVLWNLLFFFVGFCRFLCCSSYNWI 1620
            HWASYFHQASIGTHLN+QFFPY T RSC++DLNFAK                        
Sbjct: 1561 HWASYFHQASIGTHLNHQFFPYTTGRSCIQDLNFAK------------------------ 1620

Query: 1621 VAYVIFWIFLFVYFSLYLFSFFFLLIVDACILHLKYGLIDGFNKVYFQGFDAGEQTLKLF 1680
                                                            GFD GEQT+KLF
Sbjct: 1621 ------------------------------------------------GFDTGEQTVKLF 1680

Query: 1681 RKGPFSSQDLEEHLRKFFDGLPCVTMVCISLIGGDLACLLQQILHFPSSVHAWILVSHLS 1740
            R G FSSQDLEE++RKFFDGLPCV MVCISLIGGDLACLLQQILHFPSSVHAWILVSHL+
Sbjct: 1681 R-GSFSSQDLEEYVRKFFDGLPCVAMVCISLIGGDLACLLQQILHFPSSVHAWILVSHLN 1740

Query: 1741 SKRHPLVLLLPVETILKEGSEDYSNLQSDDSCERNDLAKHWQCPWGSSVIDEIAPAFRII 1800
            SKR PLVLLLPVETILKE SEDYSN QSDD CERND  KHWQCPWGSSVIDEIAPAFR I
Sbjct: 1741 SKRQPLVLLLPVETILKEDSEDYSNPQSDDICERNDSTKHWQCPWGSSVIDEIAPAFRTI 1800

Query: 1801 LEENYLSSSVFPLEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYMLLGDWSTR 1860
            LE+NYLSSSVFP EDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKY+LLGDWS R
Sbjct: 1801 LEDNYLSSSVFPSEDTKTNRMLWWKRRTKLDDCLGKLLGTIEDSWLGPWKYILLGDWSNR 1860

Query: 1861 EHFDSVLNTLVLNLKSKCKMDVNESLLKVILEGSEEVLEAFDSKLCSRKGCFIGKTRFYD 1920
            +H DSVLNTLVLNLKSKCKMDVNE LLK+ILEGSE VL  FDSKL SRKGCF+G+ RF+D
Sbjct: 1861 KHVDSVLNTLVLNLKSKCKMDVNEGLLKIILEGSENVLVGFDSKLYSRKGCFVGRARFHD 1920

Query: 1921 KERSNPFQNTLNGVDQLSALALKLIKDAKKELEGEENTCREPIILVLDYDVQMLPWENIP 1980
            KERSNPFQN LNGVD+LS LALKLI+DAKKELEGE+ T REPIILVLDYDVQMLPWENIP
Sbjct: 1921 KERSNPFQNALNGVDKLSTLALKLIQDAKKELEGEDGTSREPIILVLDYDVQMLPWENIP 1980

Query: 1981 ILQNQEVYRMPSVGCICATLDGRSHQQEQDGGIIAAFPSIDPLDAFYLLNPSGDLNSTQI 2040
            IL+NQEVYRMPSVG IC TLDGR HQQEQD GIIA FPSIDPLDAFYLLNPSGDL+STQI
Sbjct: 1981 ILKNQEVYRMPSVGSICVTLDGRLHQQEQDSGIIATFPSIDPLDAFYLLNPSGDLSSTQI 2040

Query: 2041 EFENWFKDQNLEGKAGYAPTSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS 2100
            EFENWFKDQNLEGKAGYAP SSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS
Sbjct: 2041 EFENWFKDQNLEGKAGYAPKSSELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAAS 2100

Query: 2101 LLMGCSSGSLTLNGHYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER 2160
            LLMGCSSGSLTLNG+YVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER
Sbjct: 2101 LLMGCSSGSLTLNGYYVPQGIPLSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRER 2160

Query: 2161 SGALPSSAQCDIVTKEFEAMKISSKHANKKVASKSLPAACESESSSRGHSAHSRMIGSFL 2220
            S ALPSS QCDIVTKE EAMKISSK ANKKVASKSLP  CES SSS+ HS HSRMIGSFL
Sbjct: 2161 SCALPSSVQCDIVTKELEAMKISSKLANKKVASKSLPTTCES-SSSKDHSVHSRMIGSFL 2174

Query: 2221 CEAREACNLRYLIGASPVCYGVPTSIRKKKDLT 2253
            CEAREACNLRYLIGASPVCYGVPTSIRKKK+LT
Sbjct: 2221 CEAREACNLRYLIGASPVCYGVPTSIRKKKELT 2174

BLAST of Lcy11g005290 vs. TAIR 10
Match: AT4G22970.1 (homolog of separase )

HSP 1 Score: 1691.8 bits (4380), Expect = 0.0e+00
Identity = 987/2294 (43.03%), Postives = 1368/2294 (59.63%), Query Frame = 0

Query: 2    ASPSESSLISILETADSKGISSLVSDFLQPFSDVKNPKKCKKSAKSTDDSSTIRSLAKKF 61
            +S  +  L+S+++  D+  + S  SD+L+PFS +        S K  D ++TIR+LAK+F
Sbjct: 3    SSGDDLRLLSLIDVGDN--VFSSFSDYLKPFSTLST------SRKKQDRATTIRALAKQF 62

Query: 62   LAFLNRALSILPKRLSEPSKLGNDLELALEFFEVYKLCLGCLESLTSQLSCKPYTVDVQR 121
            L FLN+++S+LPKRLS  +      E AL+ F  Y+LCL CLE +++QL+CKP+TV  QR
Sbjct: 63   LPFLNKSISLLPKRLSVANSDKEARESALDLFRAYELCLDCLELVSAQLACKPHTVQSQR 122

Query: 122  VRMVHCMEDWGLFKDAEAEGFGILERLREIDRKSKAGKLDCRVIHDGDKGGGDKDFCLLF 181
            +RM+HC++ WGL+++   E F +LE+LR  D KS+  +L   V        GD +  L+ 
Sbjct: 123  LRMIHCLDVWGLYENVYTEAFKVLEKLRGSDSKSRKSRLLPEV------QDGDAEMALVV 182

Query: 182  AEVVVTIVKCTASGRSKESGDYRRVLGLVEEVRPWFRLLDAKVSEKTQRALVTYLGKCTI 241
             + V  I +  A  +  +   YR+VL L+EEV  W R+LDAKV EK  RA+VT +GKC +
Sbjct: 183  VDAVAAIFRAVAMSQQLDDKRYRKVLLLLEEVGGWLRVLDAKVYEKLHRAMVTSMGKCAV 242

Query: 242  FLVEEVVCFGESLVSLFCRTTFAEYAKSSM-RDQIYKLARRICSTLFSLQQEQHTSMLIM 301
             LV E   F   LV  FC  T  E+ KS++ +D++YK AR + S LF  +  + +  + +
Sbjct: 243  SLVREAERFNGDLVISFCDLTVKEHYKSALSKDRVYKFAREVLSVLFGFKDRKMS--VTI 302

Query: 302  KILACVLKSITLEWKDETECTVIEFLQLICYCANKCQSESPECCCTFARHLEEMAADFHQ 361
             I   VL+S++ +++DE+   ++EF  L+ YCA+K ++     C   ++ L EMAA F +
Sbjct: 303  DISMSVLRSLSCQFEDESNENLMEFFDLVDYCAHKFRAAGDMYCAKVSKKLNEMAAIFVE 362

Query: 362  ATTPFGMNLRLYAAGLKIF---SKVPRGETHGSA----FSILVDDEDTMQSLVNLNGFLG 421
            A     + LRLY+ GL I    SK+   +   S        + DD+   QSLV+L G + 
Sbjct: 363  AIPQLNLVLRLYSTGLSITVCNSKLGEIKLEDSTDDWKIQAMFDDDARWQSLVSLLGMVD 422

Query: 422  SYFCIGCRKGNESCSNEQKDFVDQPCSHGKSNHECD--VPLLSWREAYLFSYLDAIKFLC 481
            SY       G+E               + K++  C     +  W +     Y+DA+KFLC
Sbjct: 423  SY------SGDEGNQTGSSSIGGHRNYNNKTHDSCKDRNKITCWPQ-----YVDALKFLC 482

Query: 482  QPLAESVNSERKEILAEDK---AASVLYSIQNTLYQSCDVFLFCQRRAYDAKSDGCDENV 541
            QPLA+ + S +++I+ E +   A++ L +I +   Q CD  LF QR   D K D    N 
Sbjct: 483  QPLADFIYSVKRKIVLETEMSCASAHLITIHDAFLQFCDGCLFLQRCTSD-KGDREIANN 542

Query: 542  NMLLSVVMAAFTLSIRTRLEMKRSTDLIKAVISSKWVQPFALKHLFASLNNIGIILYRNK 601
               L+  M AF +S+RT+L+++ S  L++ VI S W+Q   LK+L A+L NIGI+LYRNK
Sbjct: 543  KAFLNAAMGAFIVSLRTQLKLEISAHLVEDVIGSPWIQSQELKYLIATLYNIGIVLYRNK 602

Query: 602  QIREASKALKLCCRASWTCVRHFCVMFDDKAKPSDNEFSENAVLSFVNEVIMRSAFLVDI 661
            ++ +A +ALKLC + SW CV   C MF +++  SDN+ SE+A++ FV E   R AF +DI
Sbjct: 603  ELNKACEALKLCSKVSWRCVELHCHMFVNQSSSSDNDLSEDAIMDFVGEACNRCAFYLDI 662

Query: 662  LYQRGMHKVEKAVTEILKNWSMAEKLFNGLPVPVQLVKQWVKMQCKHHKNADPRKTTPTL 721
            L +    K+ + +  IL+NW  AE L   LP P  +VKQWVK++ + H + D   +  TL
Sbjct: 663  LQKCSRRKIRQNIVHILENWLSAEHLIRRLPGPEAIVKQWVKIERECHTDLDAAGSCTTL 722

Query: 722  NCLLQSSGMLSKAQIGALLEQELVEYAEMSGLYPEFCQSLQMKILSTLLRDVYVTPDHRL 781
              LL SS   SK  IG +LEQEL+ Y  +  L     Q  ++KI   LL+DVYVT D  +
Sbjct: 723  YSLLSSSQKKSKRGIGKILEQELLAYDRVLPLRSNLGQQTRIKIADILLKDVYVTEDMHI 782

Query: 782  EKARVLLKKARVLRFCETICLDNCIQCLSEAISTINETSGGTCSSGVLHSHQLAVAYCLR 841
            E+AR+L+ KAR+ R   T  +  CI  LSEAIS + E   G    G   SH L +AYCLR
Sbjct: 783  ERARILIWKARMTRTSGTEHITECICFLSEAISILGELHHGPNEEGSPSSHMLPIAYCLR 842

Query: 842  ALCTHETVPNSKQVLQDIDSALSVWLEISSLDSLPDDQQLILSEYMLLLLCNTFDLLSIK 901
            A CT E  PNSK+V QDI ++L++WL I SLD   D    + +E ++ LL N  DL+S+K
Sbjct: 843  AFCTQEADPNSKKVFQDISTSLNLWLRILSLD---DSGDSLPTENIIPLLYNMIDLMSVK 902

Query: 902  GCMDYYHNIYSLMIRLFKWKNVQLEKLLAVLWEGRRMSHAICIAPANEIVITQLSEHLGE 961
            GC + +H+IY L+ RLFKWKNV+LE  LA+LWE RR+SHA+C +P ++  I  LSE+  +
Sbjct: 903  GCTELHHHIYQLIFRLFKWKNVKLEVCLAMLWECRRLSHALCPSPISDAFIQTLSENCAD 962

Query: 962  LPKSFNFWTNCLKTLPGMLVGFRQNFSFLCRNNGQSCCEHEKSVQLDVTVDEVKEAASKL 1021
                 +FW +CLK     L+GF+QNF  L   +       E   Q D+T+D++K+AAS+L
Sbjct: 963  KSTCIDFWMDCLKDSKAKLIGFQQNFHDLHNKD-------EGPFQSDITIDDIKDAASEL 1022

Query: 1022 ISHTPACKSSIFLAGYLYYDLCERLISEGCLTEALSCAKEAHRLRSKLFQEKFTYSIEQH 1081
            IS      +S F A YLYYDLCERLIS G L+EALS AKEA+R+R+ +FQ+KF Y+ E+H
Sbjct: 1023 ISSASLSGNSSFAAAYLYYDLCERLISFGKLSEALSYAKEAYRIRTLIFQDKFKYTAEKH 1082

Query: 1082 PEKYCEISHVSQKPAYGIKNLQKNGLVARDVWSFDIISWDVEGCYLSPFNVLQCYLESTL 1141
             EK+ E   +S+   + IKN Q   L+A D W      WD+  CYLSP++VLQCYLESTL
Sbjct: 1083 IEKHNEDGKISEIRTFSIKNFQVYRLLATDFWPCGNFLWDINRCYLSPWSVLQCYLESTL 1142

Query: 1142 QVGLVHEMIGNGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLARKELE 1201
            QVG+++E+IGNG EAET+L WGK+ SC QSL  F VAFSSALG +Y KKQ   LA KEL+
Sbjct: 1143 QVGILNELIGNGLEAETILSWGKAFSCSQSLFPFVVAFSSALGNLYHKKQCLDLAEKELQ 1202

Query: 1202 GAKEIL--TDSITSCLKCKLVLEVIVDQHLGDLFRSMYVNANGSISEGQLENAEELYKLA 1261
             AKEIL       SC+KCKL LEV +D+ LGD+ R        S ++G L +AE L+  A
Sbjct: 1203 NAKEILIANQRDFSCVKCKLKLEVTLDKQLGDISRKQIDRV--SQTDGFL-HAESLFSAA 1262

Query: 1262 LEKLNLSAWKNFISFPDEE----------------RYLSSLTIQAERPKARKDGRKCKKT 1321
            L K   SAWK+ I    EE                   S   +  + P   +  R+  + 
Sbjct: 1263 LGKFCCSAWKSCIRSHGEEIAEEIVIDRNGGEGLGHNSSKTKLSIKEPPGNRGSRRGGRA 1322

Query: 1322 TNASRPFQSDQCVNSQSNVRLTRSRCRTIQGQSTSNSNEVEVDLSVHLKSNIPDLSSASC 1381
                     DQ + S+   RLTRS   +++ Q  + SN  EV   V  K N+ D S  S 
Sbjct: 1323 NKTC--LSKDQDLISEPTSRLTRSMRHSLREQCQNRSNVPEV---VSKKPNLCDRSVGSR 1382

Query: 1382 QKQSYLQVNSCTPTFGCGASCKNGKVGCWQCLPTEIMEAGEMNNFIYLKWEFVRRRLLLR 1441
             ++  L  ++  P F     C   K    QCL  E+ E+G +NN + LKWE   R+L   
Sbjct: 1383 GERVLLDTSNALPGF-----CICYKEKRQQCLSEEVTESGSLNNLVSLKWELCHRKLASS 1442

Query: 1442 HLSGLGKCSEIRGQIHQTHETILKSMSILVSRNLFFQAHYAVEPMILLELVGKEVHRDMF 1501
             L  LGKC    G+IH  HE +L S+S+L      + +H       LLE +GKEV RD+F
Sbjct: 1443 ILVSLGKCLGDSGRIHLAHEALLHSISVLFKST--WSSHNQPSVSQLLEFIGKEVTRDVF 1502

Query: 1502 AVERASVLYNICCFSLKSYKYDDSKITCCPLSQVQSKTLVSWLMVALVLCCEVPVLFQKV 1561
            AV+RA +LYN+C  +L++Y    S+  CC L  +    LVSWLM+A VL  EVP+LFQKV
Sbjct: 1503 AVDRAIILYNLCWLNLRNYHCRKSRSICCDLFHIPFTKLVSWLMLAFVLSGEVPILFQKV 1562

Query: 1562 SKLLAVIHVISSSSELFSLPSSNKILTDSHWASYFHQASIGTHLNYQFFPYMTRR---SC 1621
            S+LLA ++++SSS+  F+  S    L+ SHW S+FHQAS+GTHL+Y F   ++++    C
Sbjct: 1563 SRLLASLYLLSSSNSEFTFESDGNELSASHWVSFFHQASLGTHLSYHFISNLSQKHKSQC 1622

Query: 1622 VEDLNFAKVLWNLLFFFVGFCRFLCCSSYNWIVAYVIFWIFLFVYFSLYLFSFFFLLIVD 1681
            + D                 C    CSS                                
Sbjct: 1623 LSDKE---------------CTEATCSS-------------------------------- 1682

Query: 1682 ACILHLKYGLIDGFNKVYFQGFDAGEQTLKLFRKGPFSSQDLEEHLRKFFDGLPCVTMVC 1741
             C++                      + L L R  P  +QDL +  ++FF  LP  T++C
Sbjct: 1683 -CMV---------------------PEDLDLPRLAPDRTQDLVQFAKEFFINLPSSTIIC 1742

Query: 1742 ISLIGGDLACLLQQILHFPSSVHAWILVSHLSSKRHPLVLLLPVETILKEGSEDYSNLQS 1801
            ISL+GG L  LLQ+++H  S V AW+L+S L+ +  P+  LLPV++I+++ S+D +NL S
Sbjct: 1743 ISLLGGALNQLLQELMHIRSPVCAWVLISRLNPESQPVATLLPVDSIVEDMSDDSANLSS 1802

Query: 1802 DDSCERNDLAKHWQCPWGSSVIDEIAPAFRIILEENYLSSSVFPLEDTKTNRMLWWKRRT 1861
             ++ +   L   W CPWG++V+DE+APAF+ ILEE++ SSS    EDT  +R LWWK+R 
Sbjct: 1803 TEATQVKSLKGPWLCPWGTTVVDEVAPAFKSILEESHSSSSTTE-EDTIESRGLWWKKRK 1862

Query: 1862 KLDDCLGKLLGTIEDSWLGPWKYMLLGDWSTREHFDSVLNTLVLNLKSKCKMDVNESLLK 1921
            KL+  LG  L  +E SWLGPW+ +LLG+WS  +  DS    LV +LKSKCKM+VNE LLK
Sbjct: 1863 KLNHRLGIFLRNLEASWLGPWRCLLLGEWSNYKLPDSAQKKLVNDLKSKCKMEVNEMLLK 1922

Query: 1922 VILEGSEEVL--EAFDSKLCSRKGCFIGKTRF-YDKERSNPFQNTLNGVDQLSALALKLI 1981
            VIL G  +    EA  ++L  R GC++G+  + Y+++         N  +    LALKLI
Sbjct: 1923 VILGGGTDNFKGEACVAQLSLRNGCYVGRGGYLYEEDSCKTPTAASNISESRHELALKLI 1982

Query: 1982 KDAKKEL---EGEENTCREPIILVLDYDVQMLPWENIPILQNQEVYRMPSVGCICATLDG 2041
             DA  +L   +G EN  REPIILVLD +VQMLPWENIPIL+ QEVYRMPSVGCI A L  
Sbjct: 1983 HDAASKLGQQDGHEN--REPIILVLDPEVQMLPWENIPILRKQEVYRMPSVGCISAVLKK 2042

Query: 2042 RSHQQEQDGGIIAAFPSIDPLDAFYLLNPSGDLNSTQIEFENWFKDQNLEGKAGYAPTSS 2101
            RS Q E     +A+FP IDPLD+FYLLNP GDL  TQ+ FE+WF+DQN EGKAG  P++ 
Sbjct: 2043 RSLQGEPAKSHVASFPLIDPLDSFYLLNPGGDLTDTQVTFESWFRDQNFEGKAGSEPSAI 2102

Query: 2102 ELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAASLLMGCSSGSLTLNGHYVPQGIP 2161
            EL E L++ DLF+YFGHGSGAQYIPR EI+KLD C+A+ LMGCSSGSL L G Y+PQG+P
Sbjct: 2103 ELTEALETHDLFLYFGHGSGAQYIPRREIEKLDNCSATFLMGCSSGSLWLKGCYIPQGVP 2162

Query: 2162 LSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRERSGALPSS--AQCDIVTKEFEAM 2221
            LSYL  GSP IVA LW+VTD+DIDRFGKA+LEAWL+ERS +      +QC+ +  +  AM
Sbjct: 2163 LSYLLGGSPAIVATLWDVTDRDIDRFGKALLEAWLQERSDSSSEGGCSQCESLANDLAAM 2171

Query: 2222 KISSKHANKKVASKSLPAACESESSSR--GHSAHSRMIGSFLCEAREACNLRYLIGASPV 2252
             +     ++K +S++ PA  + + S +   +  H R IGSF+  AR+ACNL+YLIGA+PV
Sbjct: 2223 TLKGTKRSRKPSSRNKPAQSDVDGSGKIECNHKHRRKIGSFIAAARDACNLQYLIGAAPV 2171

BLAST of Lcy11g005290 vs. TAIR 10
Match: AT4G22970.2 (homolog of separase )

HSP 1 Score: 1685.2 bits (4363), Expect = 0.0e+00
Identity = 986/2294 (42.98%), Postives = 1366/2294 (59.55%), Query Frame = 0

Query: 2    ASPSESSLISILETADSKGISSLVSDFLQPFSDVKNPKKCKKSAKSTDDSSTIRSLAKKF 61
            +S  +  L+S+++  D+  + S  SD+L+PFS +        S K  D ++TIR+LAK+F
Sbjct: 3    SSGDDLRLLSLIDVGDN--VFSSFSDYLKPFSTLST------SRKKQDRATTIRALAKQF 62

Query: 62   LAFLNRALSILPKRLSEPSKLGNDLELALEFFEVYKLCLGCLESLTSQLSCKPYTVDVQR 121
            L FLN+++S+LPKRLS  +      E AL+ F  Y+LCL CLE +++QL+CKP+TV  QR
Sbjct: 63   LPFLNKSISLLPKRLSVANSDKEARESALDLFRAYELCLDCLELVSAQLACKPHTVQSQR 122

Query: 122  VRMVHCMEDWGLFKDAEAEGFGILERLREIDRKSKAGKLDCRVIHDGDKGGGDKDFCLLF 181
            +RM+HC++ WGL+++   E F +LE+LR  D KS+  +L   V        GD +  L+ 
Sbjct: 123  LRMIHCLDVWGLYENVYTEAFKVLEKLRGSDSKSRKSRLLPEV------QDGDAEMALVV 182

Query: 182  AEVVVTIVKCTASGRSKESGDYRRVLGLVEEVRPWFRLLDAKVSEKTQRALVTYLGKCTI 241
             + V  I +  A  +  +   YR+VL L+EEV  W R+LDAKV EK  RA+VT +GKC +
Sbjct: 183  VDAVAAIFRAVAMSQQLDDKRYRKVLLLLEEVGGWLRVLDAKVYEKLHRAMVTSMGKCAV 242

Query: 242  FLVEEVVCFGESLVSLFCRTTFAEYAKSSM-RDQIYKLARRICSTLFSLQQEQHTSMLIM 301
             LV E   F   LV  FC  T  E+ KS++ +D++YK AR + S LF  +  + +  + +
Sbjct: 243  SLVREAERFNGDLVISFCDLTVKEHYKSALSKDRVYKFAREVLSVLFGFKDRKMS--VTI 302

Query: 302  KILACVLKSITLEWKDETECTVIEFLQLICYCANKCQSESPECCCTFARHLEEMAADFHQ 361
             I   VL+S++ +++DE+   ++EF  L+ YCA+K ++     C   ++ L EMAA F +
Sbjct: 303  DISMSVLRSLSCQFEDESNENLMEFFDLVDYCAHKFRAAGDMYCAKVSKKLNEMAAIFVE 362

Query: 362  ATTPFGMNLRLYAAGLKIF---SKVPRGETHGSA----FSILVDDEDTMQSLVNLNGFLG 421
            A     + LRLY+ GL I    SK+   +   S        + DD+   QSLV+L G + 
Sbjct: 363  AIPQLNLVLRLYSTGLSITVCNSKLGEIKLEDSTDDWKIQAMFDDDARWQSLVSLLGMVD 422

Query: 422  SYFCIGCRKGNESCSNEQKDFVDQPCSHGKSNHECD--VPLLSWREAYLFSYLDAIKFLC 481
            SY       G+E               + K++  C     +  W +     Y+DA+KFLC
Sbjct: 423  SY------SGDEGNQTGSSSIGGHRNYNNKTHDSCKDRNKITCWPQ-----YVDALKFLC 482

Query: 482  QPLAESVNSERKEILAEDK---AASVLYSIQNTLYQSCDVFLFCQRRAYDAKSDGCDENV 541
            QPLA+ + S +++I+ E +   A++ L +I +   Q CD  LF QR   D K D    N 
Sbjct: 483  QPLADFIYSVKRKIVLETEMSCASAHLITIHDAFLQFCDGCLFLQRCTSD-KGDREIANN 542

Query: 542  NMLLSVVMAAFTLSIRTRLEMKRSTDLIKAVISSKWVQPFALKHLFASLNNIGIILYRNK 601
               L+  M AF +S+RT+L+++ S  L++ VI S W+Q   LK+L A+L NIGI+LYRNK
Sbjct: 543  KAFLNAAMGAFIVSLRTQLKLEISAHLVEDVIGSPWIQSQELKYLIATLYNIGIVLYRNK 602

Query: 602  QIREASKALKLCCRASWTCVRHFCVMFDDKAKPSDNEFSENAVLSFVNEVIMRSAFLVDI 661
            ++ +A +ALKLC + SW CV   C MF +++  SDN+ SE+A++ FV E   R AF +DI
Sbjct: 603  ELNKACEALKLCSKVSWRCVELHCHMFVNQSSSSDNDLSEDAIMDFVGEACNRCAFYLDI 662

Query: 662  LYQRGMHKVEKAVTEILKNWSMAEKLFNGLPVPVQLVKQWVKMQCKHHKNADPRKTTPTL 721
            L +    K+ + +  IL+NW  AE L   LP P  +VKQWVK++ + H + D   +  TL
Sbjct: 663  LQKCSRRKIRQNIVHILENWLSAEHLIRRLPGPEAIVKQWVKIERECHTDLDAAGSCTTL 722

Query: 722  NCLLQSSGMLSKAQIGALLEQELVEYAEMSGLYPEFCQSLQMKILSTLLRDVYVTPDHRL 781
              LL SS   SK  IG +LEQEL+ Y  +  L     Q  ++KI   LL+DVYVT D  +
Sbjct: 723  YSLLSSSQKKSKRGIGKILEQELLAYDRVLPLRSNLGQQTRIKIADILLKDVYVTEDMHI 782

Query: 782  EKARVLLKKARVLRFCETICLDNCIQCLSEAISTINETSGGTCSSGVLHSHQLAVAYCLR 841
            E+AR+L+ KAR+ R   T  +  CI  LSEAIS + E   G    G   SH L +AYCLR
Sbjct: 783  ERARILIWKARMTRTSGTEHITECICFLSEAISILGELHHGPNEEGSPSSHMLPIAYCLR 842

Query: 842  ALCTHETVPNSKQVLQDIDSALSVWLEISSLDSLPDDQQLILSEYMLLLLCNTFDLLSIK 901
            A CT E  PNSK+V QDI ++L++WL I SLD   D    + +E ++ LL N  DL+S+K
Sbjct: 843  AFCTQEADPNSKKVFQDISTSLNLWLRILSLD---DSGDSLPTENIIPLLYNMIDLMSVK 902

Query: 902  GCMDYYHNIYSLMIRLFKWKNVQLEKLLAVLWEGRRMSHAICIAPANEIVITQLSEHLGE 961
            GC + +H+IY L+ RLFKWKNV+LE  LA+LWE RR+SHA+C +P ++  I  LSE+  +
Sbjct: 903  GCTELHHHIYQLIFRLFKWKNVKLEVCLAMLWECRRLSHALCPSPISDAFIQTLSENCAD 962

Query: 962  LPKSFNFWTNCLKTLPGMLVGFRQNFSFLCRNNGQSCCEHEKSVQLDVTVDEVKEAASKL 1021
                 +FW +CLK     L+GF+QNF  L   +       E   Q D+T+D++K+AAS+L
Sbjct: 963  KSTCIDFWMDCLKDSKAKLIGFQQNFHDLHNKD-------EGPFQSDITIDDIKDAASEL 1022

Query: 1022 ISHTPACKSSIFLAGYLYYDLCERLISEGCLTEALSCAKEAHRLRSKLFQEKFTYSIEQH 1081
            IS      +S F A YLYYDLCERLIS G L+EALS AKEA+R+R+ +FQ+KF Y+ E+H
Sbjct: 1023 ISSASLSGNSSFAAAYLYYDLCERLISFGKLSEALSYAKEAYRIRTLIFQDKFKYTAEKH 1082

Query: 1082 PEKYCEISHVSQKPAYGIKNLQKNGLVARDVWSFDIISWDVEGCYLSPFNVLQCYLESTL 1141
             EK+ E   +S+   + IKN Q   L+A D W      WD+  CYLSP++VLQCYLESTL
Sbjct: 1083 IEKHNEDGKISEIRTFSIKNFQVYRLLATDFWPCGNFLWDINRCYLSPWSVLQCYLESTL 1142

Query: 1142 QVGLVHEMIGNGSEAETLLQWGKSISCLQSLPLFEVAFSSALGKVYRKKQLWSLARKELE 1201
            QVG+++E+IGNG EAET+L WGK+ SC QSL  F VAFSSALG +Y KKQ   LA KEL+
Sbjct: 1143 QVGILNELIGNGLEAETILSWGKAFSCSQSLFPFVVAFSSALGNLYHKKQCLDLAEKELQ 1202

Query: 1202 GAKEIL--TDSITSCLKCKLVLEVIVDQHLGDLFRSMYVNANGSISEGQLENAEELYKLA 1261
             AKEIL       SC+KCKL LEV +D+ LGD+ R        S ++G L +AE L+  A
Sbjct: 1203 NAKEILIANQRDFSCVKCKLKLEVTLDKQLGDISRKQIDRV--SQTDGFL-HAESLFSAA 1262

Query: 1262 LEKLNLSAWKNFISFPDEE----------------RYLSSLTIQAERPKARKDGRKCKKT 1321
            L K   SAWK+ I    EE                   S   +  + P   +  R+  + 
Sbjct: 1263 LGKFCCSAWKSCIRSHGEEIAEEIVIDRNGGEGLGHNSSKTKLSIKEPPGNRGSRRGGRA 1322

Query: 1322 TNASRPFQSDQCVNSQSNVRLTRSRCRTIQGQSTSNSNEVEVDLSVHLKSNIPDLSSASC 1381
                     DQ + S+   RLTRS   +++ Q  + SN  EV   V  K N+ D S  S 
Sbjct: 1323 NKTC--LSKDQDLISEPTSRLTRSMRHSLREQCQNRSNVPEV---VSKKPNLCDRSVGSR 1382

Query: 1382 QKQSYLQVNSCTPTFGCGASCKNGKVGCWQCLPTEIMEAGEMNNFIYLKWEFVRRRLLLR 1441
             ++  L  ++  P F     C   K    QCL  E+ E+G +NN + LKWE   R+L   
Sbjct: 1383 GERVLLDTSNALPGF-----CICYKEKRQQCLSEEVTESGSLNNLVSLKWELCHRKLASS 1442

Query: 1442 HLSGLGKCSEIRGQIHQTHETILKSMSILVSRNLFFQAHYAVEPMILLELVGKEVHRDMF 1501
             L  LGKC    G+IH  HE +L S+S+L      + +H       LLE +GKEV RD+F
Sbjct: 1443 ILVSLGKCLGDSGRIHLAHEALLHSISVLFKST--WSSHNQPSVSQLLEFIGKEVTRDVF 1502

Query: 1502 AVERASVLYNICCFSLKSYKYDDSKITCCPLSQVQSKTLVSWLMVALVLCCEVPVLFQKV 1561
            AV+RA +LYN+C  +L++Y    S+  CC L  +    LVSWLM+A VL  EVP+LFQKV
Sbjct: 1503 AVDRAIILYNLCWLNLRNYHCRKSRSICCDLFHIPFTKLVSWLMLAFVLSGEVPILFQKV 1562

Query: 1562 SKLLAVIHVISSSSELFSLPSSNKILTDSHWASYFHQASIGTHLNYQFFPYMTRR---SC 1621
            S+LLA ++++SSS+  F+  S    L+ SHW S+FHQAS+GTHL+Y F   ++++    C
Sbjct: 1563 SRLLASLYLLSSSNSEFTFESDGNELSASHWVSFFHQASLGTHLSYHFISNLSQKHKSQC 1622

Query: 1622 VEDLNFAKVLWNLLFFFVGFCRFLCCSSYNWIVAYVIFWIFLFVYFSLYLFSFFFLLIVD 1681
            + D                 C    CSS                                
Sbjct: 1623 LSDKE---------------CTEATCSS-------------------------------- 1682

Query: 1682 ACILHLKYGLIDGFNKVYFQGFDAGEQTLKLFRKGPFSSQDLEEHLRKFFDGLPCVTMVC 1741
             C++                      + L L R  P  +QDL +  ++FF  LP  T++C
Sbjct: 1683 -CMV---------------------PEDLDLPRLAPDRTQDLVQFAKEFFINLPSSTIIC 1742

Query: 1742 ISLIGGDLACLLQQILHFPSSVHAWILVSHLSSKRHPLVLLLPVETILKEGSEDYSNLQS 1801
            ISL+GG L  LLQ+++H  S V AW+L+S L+ +  P+  LLPV++I++   +D +NL S
Sbjct: 1743 ISLLGGALNQLLQELMHIRSPVCAWVLISRLNPESQPVATLLPVDSIVE---DDSANLSS 1802

Query: 1802 DDSCERNDLAKHWQCPWGSSVIDEIAPAFRIILEENYLSSSVFPLEDTKTNRMLWWKRRT 1861
             ++ +   L   W CPWG++V+DE+APAF+ ILEE++ SSS    EDT  +R LWWK+R 
Sbjct: 1803 TEATQVKSLKGPWLCPWGTTVVDEVAPAFKSILEESHSSSSTTE-EDTIESRGLWWKKRK 1862

Query: 1862 KLDDCLGKLLGTIEDSWLGPWKYMLLGDWSTREHFDSVLNTLVLNLKSKCKMDVNESLLK 1921
            KL+  LG  L  +E SWLGPW+ +LLG+WS  +  DS    LV +LKSKCKM+VNE LLK
Sbjct: 1863 KLNHRLGIFLRNLEASWLGPWRCLLLGEWSNYKLPDSAQKKLVNDLKSKCKMEVNEMLLK 1922

Query: 1922 VILEGSEEVL--EAFDSKLCSRKGCFIGKTRF-YDKERSNPFQNTLNGVDQLSALALKLI 1981
            VIL G  +    EA  ++L  R GC++G+  + Y+++         N  +    LALKLI
Sbjct: 1923 VILGGGTDNFKGEACVAQLSLRNGCYVGRGGYLYEEDSCKTPTAASNISESRHELALKLI 1982

Query: 1982 KDAKKEL---EGEENTCREPIILVLDYDVQMLPWENIPILQNQEVYRMPSVGCICATLDG 2041
             DA  +L   +G EN  REPIILVLD +VQMLPWENIPIL+ QEVYRMPSVGCI A L  
Sbjct: 1983 HDAASKLGQQDGHEN--REPIILVLDPEVQMLPWENIPILRKQEVYRMPSVGCISAVLKK 2042

Query: 2042 RSHQQEQDGGIIAAFPSIDPLDAFYLLNPSGDLNSTQIEFENWFKDQNLEGKAGYAPTSS 2101
            RS Q E     +A+FP IDPLD+FYLLNP GDL  TQ+ FE+WF+DQN EGKAG  P++ 
Sbjct: 2043 RSLQGEPAKSHVASFPLIDPLDSFYLLNPGGDLTDTQVTFESWFRDQNFEGKAGSEPSAI 2102

Query: 2102 ELIEELKSRDLFIYFGHGSGAQYIPRHEIQKLDACAASLLMGCSSGSLTLNGHYVPQGIP 2161
            EL E L++ DLF+YFGHGSGAQYIPR EI+KLD C+A+ LMGCSSGSL L G Y+PQG+P
Sbjct: 2103 ELTEALETHDLFLYFGHGSGAQYIPRREIEKLDNCSATFLMGCSSGSLWLKGCYIPQGVP 2162

Query: 2162 LSYLKAGSPVIVANLWEVTDKDIDRFGKAILEAWLRERSGALPSS--AQCDIVTKEFEAM 2221
            LSYL  GSP IVA LW+VTD+DIDRFGKA+LEAWL+ERS +      +QC+ +  +  AM
Sbjct: 2163 LSYLLGGSPAIVATLWDVTDRDIDRFGKALLEAWLQERSDSSSEGGCSQCESLANDLAAM 2168

Query: 2222 KISSKHANKKVASKSLPAACESESSSR--GHSAHSRMIGSFLCEAREACNLRYLIGASPV 2252
             +     ++K +S++ PA  + + S +   +  H R IGSF+  AR+ACNL+YLIGA+PV
Sbjct: 2223 TLKGTKRSRKPSSRNKPAQSDVDGSGKIECNHKHRRKIGSFIAAARDACNLQYLIGAAPV 2168

BLAST of Lcy11g005290 vs. TAIR 10
Match: AT5G28550.1 (BEST Arabidopsis thaliana protein match is: homolog of separase (TAIR:AT4G22970.2); Has 25 Blast hits to 25 proteins in 6 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 25; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 134.0 bits (336), Expect = 1.5e-30
Identity = 72/174 (41.38%), Postives = 104/174 (59.77%), Query Frame = 0

Query: 531 LLSVVMAAFTLSIRT--RLEMKR-----------STDLIKAVISSKWVQPFALKHLFASL 590
           LL+VVMAAF  S+RT   +++KR           +  L++ VI+S W+ P  LK LFAS 
Sbjct: 80  LLNVVMAAFIFSMRTLRNVDVKRLLAIVTNQWKITNRLVEDVIASPWISPPELKFLFASF 139

Query: 591 NNIGIILYRNKQIREASKALKLCCRASWTCVRHFCVMFDDKAKPSDNEFSENAVLSFVNE 650
           ++I +  Y NK + +AS   KLC R  WTCVR  C ++ +K+  S++   + A++ FV+E
Sbjct: 140 HDISVAFYNNKHLEKASMVFKLCIRTVWTCVRLLCQIYVNKSDLSEDCLPKEAIIDFVSE 199

Query: 651 VIMRSAFLVDILYQRGMHKVEKAVTEILKNWSMAEKLFNGLPVPVQLVKQWVKM 692
              +SAF +D L+Q G  + EK    IL+NW  AE L   LP P  +VKQWVK+
Sbjct: 200 ACSKSAFYLDNLHQHGARETEKLHVFILENWPEAEDLIKKLPDPTPIVKQWVKL 253

BLAST of Lcy11g005290 vs. TAIR 10
Match: AT4G24300.1 (Peptidase C50, separase )

HSP 1 Score: 75.1 bits (183), Expect = 8.1e-13
Identity = 44/143 (30.77%), Postives = 79/143 (55.24%), Query Frame = 0

Query: 184 VVVTIVKCTASGRSKESGDYRRVLGLVEEVRPWFRLLDAKVSEKTQRALVTYLGKCTIFL 243
           VV +IV+  A  R      Y R + L++ ++PW R LDA    K  + +++ +G+C + +
Sbjct: 2   VVASIVRSVAMSRDMNDLQYLRGISLLQGLKPWLRYLDANEHHKFLKLVLSDMGECALSV 61

Query: 244 VEEVVCFGESLVSLFCRTTFAEYAKSSM-RDQIYKLARRICSTLFSLQQEQHTSMLIMKI 303
           + E   F E+ V  FC +T  EY+ S + +   YK +R++ S LF   +E  TS L +KI
Sbjct: 62  IREAERFDEAFVHSFCISTLEEYSVSPLPKCHFYKFSRQVLSLLFP-SKEAKTS-LNLKI 121

Query: 304 LACVLKSITLEWKDETECTVIEF 326
           +  VLK +  E++++   + + +
Sbjct: 122 MMSVLKFVAGEFQNQQSSSRVRY 142