Lcy11g004480 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy11g004480
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Trypsin inhibitor 5
Location	Chr11: 4388157 .. 4388685 (+)
RNA-Seq Expression	Lcy11g004480
Synteny	Lcy11g004480

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTCTAGATCTCTATAAATACAGAGGGTTGAGAAAGCAGTGGGCAAAAGCAAGAGATCAAAAGCAAATAAGATTCAAAAGTGTGTGTGAGAGAGAGAAGATAATGGAGTGGAAGAAAGTTGCTCTGATGGCAATGGTGGGAATGTTGCTGATGGCGAGTGTTGCAGAGTCGAGCGGCGTGGTGGAGGTGATTGAACTGATTTCCGACGGAGGGAATGATTTACCAAGAAAGATCATGAGCGGTCGCCATGGAGGAATTTGCCCAAGAATCCTTATGCCCTGCAAGACCGACGATGACTGCATGCTTGACTGTCGCTGCCTGTCCAACGGCTATTGTGGTTGAACAAACTATGTTCTGTCGGAATCTCTCTGTGTGTGCTGTTTGTCTTTTCTCCTTCTTCTATGAAAATAAAGTGTTGTCTCTTTGATCACTCTTCCTCTATGTGGGAGTGTATGTGATTAATTGAAACATCTGTGTAATGTAATCTTTTCTATGTTCTATGAAGCTCTACTATCTTCTTTGAGTC

mRNA sequence

ATGGGTCTAGATCTCTATAAATACAGAGGGTTGAGAAAGCAGTGGGCAAAAGCAAGAGATCAAAAGCAAATAAGATTCAAAAGTGTGTGTGAGAGAGAGAAGATAATGGAGTGGAAGAAAGTTGCTCTGATGGCAATGGTGGGAATGTTGCTGATGGCGAGTGTTGCAGAGTCGAGCGGCGTGGTGGAGGTGATTGAACTGATTTCCGACGGAGGGAATGATTTACCAAGAAAGATCATGAGCGGTCGCCATGGAGGAATTTGCCCAAGAATCCTTATGCCCTGCAAGACCGACGATGACTGCATGCTTGACTGTCGCTGCCTGTCCAACGGCTATTGTGGTTGAACAAACTATGTTCTGTCGGAATCTCTCTGTGTGTGCTGTTTGTCTTTTCTCCTTCTTCTATGAAAATAAAGTGTTGTCTCTTTGATCACTCTTCCTCTATGTGGGAGTGTATGTGATTAATTGAAACATCTGTGTAATGTAATCTTTTCTATGTTCTATGAAGCTCTACTATCTTCTTTGAGTC

Coding sequence (CDS)

ATGGGTCTAGATCTCTATAAATACAGAGGGTTGAGAAAGCAGTGGGCAAAAGCAAGAGATCAAAAGCAAATAAGATTCAAAAGTGTGTGTGAGAGAGAGAAGATAATGGAGTGGAAGAAAGTTGCTCTGATGGCAATGGTGGGAATGTTGCTGATGGCGAGTGTTGCAGAGTCGAGCGGCGTGGTGGAGGTGATTGAACTGATTTCCGACGGAGGGAATGATTTACCAAGAAAGATCATGAGCGGTCGCCATGGAGGAATTTGCCCAAGAATCCTTATGCCCTGCAAGACCGACGATGACTGCATGCTTGACTGTCGCTGCCTGTCCAACGGCTATTGTGGTTGA

Protein sequence

MGLDLYKYRGLRKQWAKARDQKQIRFKSVCEREKIMEWKKVALMAMVGMLLMASVAESSGVVEVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLSNGYCG

Homology

[Close]

BLAST of Lcy11g004480 vs. ExPASy Swiss-Prot
Match: P34950 (Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 1.2e-31
Identity = 63/63 (100.00%), Postives = 63/63 (100.00%), Query Frame = 0


Query: 52  MASVAESSGVVEVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLSNG 111
           MASVAESSGVVEVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLSNG
Sbjct: 1   MASVAESSGVVEVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLSNG 60

Query: 112 YCG 115
           YCG
Sbjct: 61  YCG 63

[Close]

BLAST of Lcy11g004480 vs. ExPASy Swiss-Prot
Match: Q43667 (Trypsin inhibitor 1 OS=Trichosanthes kirilowii OX=3677 PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 4.4e-13
Identity = 37/67 (55.22%), Postives = 46/67 (68.66%), Query Frame = 0


Query: 52  MASVAES----SGVVEVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRC 111
           MA+  ES    +G  EVI+L+SDG N+   K+M G     CPRILMPCK +DDC+  C+C
Sbjct: 1   MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMEGVVA--CPRILMPCKVNDDCLRGCKC 60

Query: 112 LSNGYCG 115
           LSNGYCG
Sbjct: 61  LSNGYCG 65

[Close]

BLAST of Lcy11g004480 vs. ExPASy Swiss-Prot
Match: P35628 (Trypsin inhibitor 4 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.4e-06
Identity = 19/28 (67.86%), Postives = 23/28 (82.14%), Query Frame = 0


Query: 87  ICPRILMPCKTDDDCMLDCRCLSNGYCG 115
           ICPRILMPC +D DC+ +C CL NG+CG
Sbjct: 1   ICPRILMPCSSDSDCLAECICLENGFCG 28

[Close]

BLAST of Lcy11g004480 vs. ExPASy Swiss-Prot
Match: P01074 (Trypsin inhibitor 1 OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 8.9e-06
Identity = 18/28 (64.29%), Postives = 22/28 (78.57%), Query Frame = 0


Query: 87  ICPRILMPCKTDDDCMLDCRCLSNGYCG 115
           +CPRILM CK D DC+ +C CL +GYCG
Sbjct: 2   VCPRILMECKKDSDCLAECVCLEHGYCG 29

[Close]

BLAST of Lcy11g004480 vs. ExPASy Swiss-Prot
Match: P07853 (Trypsin inhibitor 4 OS=Cucurbita maxima OX=3661 PE=1 SV=2)

HSP 1 Score: 51.2 bits (121), Expect = 8.9e-06
Identity = 18/28 (64.29%), Postives = 22/28 (78.57%), Query Frame = 0


Query: 87  ICPRILMPCKTDDDCMLDCRCLSNGYCG 115
           +CPRILM CK D DC+ +C CL +GYCG
Sbjct: 5   VCPRILMKCKKDSDCLAECVCLEHGYCG 32

[Close]

BLAST of Lcy11g004480 vs. ExPASy TrEMBL
Match: A0A6J1JBL8 (trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482991 PE=3 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 8.9e-17
Identity = 49/81 (60.49%), Postives = 61/81 (75.31%), Query Frame = 0


Query: 36  MEWKKVALMAMVGMLLMASVAESSGV--VEVIELISDGGNDLPRKIMSGRHGGICPRILM 95
           MEWK+ AL+AMVG+LLMA+ AES+ V   EVI+L+SDG ND PRK+M+G  G  CPRILM
Sbjct: 1   MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGVQG--CPRILM 60

Query: 96  PCKTDDDCMLDCRCLSNGYCG 115
            C+ D DC+  C C  NG+CG
Sbjct: 61  ECELDSDCLPGCICRPNGFCG 79

[Close]

BLAST of Lcy11g004480 vs. ExPASy TrEMBL
Match: A0A5D3E0W0 (Trypsin inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00600 PE=3 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 4.1e-14
Identity = 47/82 (57.32%), Postives = 56/82 (68.29%), Query Frame = 0


Query: 36  MEWKKVALMAMVGMLLMASVAESSGV---VEVIELISDGGNDLPRKIMSGRHGGICPRIL 95
           MEWKK+AL+AM+GMLLMA+  ES G     E+I+L+SDG N+      SG  G  CPRIL
Sbjct: 1   MEWKKIALVAMMGMLLMATFTESVGFGVDEEIIQLVSDGVNE-----YSGHPG--CPRIL 60

Query: 96  MPCKTDDDCMLDCRCLSNGYCG 115
           M CKTD DC+  C C  NGYCG
Sbjct: 61  MKCKTDRDCLAGCTCKRNGYCG 75

[Close]

BLAST of Lcy11g004480 vs. ExPASy TrEMBL
Match: A0A0A0KY34 (Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 1.2e-13
Identity = 45/82 (54.88%), Postives = 55/82 (67.07%), Query Frame = 0


Query: 36  MEWKKVALMAMVGMLLMASVAESSGV---VEVIELISDGGNDLPRKIMSGRHGGICPRIL 95
           MEWKK+AL+AM+GMLLMA+  ES G+    E+I+L+SDG N+  + I      G CPRIL
Sbjct: 1   MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPG-CPRIL 60

Query: 96  MPCKTDDDCMLDCRCLSNGYCG 115
           M CKTD DC   C C   GYCG
Sbjct: 61  MKCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of Lcy11g004480 vs. ExPASy TrEMBL
Match: A0A0A0KT01 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000805 PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.0e-12
Identity = 41/80 (51.25%), Postives = 55/80 (68.75%), Query Frame = 0


Query: 36  MEWKKVALMAMVGMLLMASVAES-SGVVEVIELISDGGNDLPRKIMSGRHGGICPRILMP 95
           MEWKK+ ++A+VGML+MA++ E     +E   +     N+LPRK+M+     +CPRIL  
Sbjct: 1   MEWKKIGMLAVVGMLVMAALVEDCHAAIESESVKGLNKNELPRKMMN-EETRMCPRILEE 60

Query: 96  CKTDDDCMLDCRCLSNGYCG 115
           C TDDDCM DC CLSNG+CG
Sbjct: 61  CTTDDDCMNDCICLSNGFCG 79

[Close]

BLAST of Lcy11g004480 vs. ExPASy TrEMBL
Match: A0A4P2SJP1 (Trypsin inhibitor I1 OS=Trichosanthes kirilowii OX=3677 PE=2 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 3.3e-11
Identity = 38/67 (56.72%), Postives = 47/67 (70.15%), Query Frame = 0


Query: 52  MASVAES----SGVVEVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRC 111
           MA+  ES    +G  EVI+L+SDG N+   K+M G  G  CPRILMPCK +DDC+  C+C
Sbjct: 1   MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMKGVVG--CPRILMPCKVNDDCLRGCKC 60

Query: 112 LSNGYCG 115
           LSNGYCG
Sbjct: 61  LSNGYCG 65

[Close]

BLAST of Lcy11g004480 vs. NCBI nr
Match: P34950.1 (RecName: Full=Trypsin inhibitor 5; AltName: Full=TGT-II; AltName: Full=Trypsin inhibitor II; Flags: Precursor [Luffa aegyptiaca] >AAA33407.1 trypsin inhibitor [Luffa aegyptiaca])

HSP 1 Score: 137.1 bits (344), Expect = 9.4e-29
Identity = 63/63 (100.00%), Postives = 63/63 (100.00%), Query Frame = 0


Query: 52  MASVAESSGVVEVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLSNG 111
           MASVAESSGVVEVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLSNG
Sbjct: 1   MASVAESSGVVEVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLSNG 60

Query: 112 YCG 115
           YCG
Sbjct: 61  YCG 63

[Close]

BLAST of Lcy11g004480 vs. NCBI nr
Match: KAG6576915.1 (hypothetical protein SDJN03_24489, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 98.6 bits (244), Expect = 3.7e-17
Identity = 56/102 (54.90%), Postives = 71/102 (69.61%), Query Frame = 0


Query: 17  KARDQKQIRFKSVC--EREKIMEWKKVALMAMVGMLLMASVAESSGV--VEVIELISDGG 76
           K R  +  R K +   +REK MEWK+ AL+AMVG+LLMA+ AES+ V   EVI+L+SDG 
Sbjct: 63  KCRSNRYERVKGIMGNKREK-MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGV 122

Query: 77  NDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLSNGYCG 115
           ND PRK+M+G  G  CPRILM C+ D DC+  C C  NG+CG
Sbjct: 123 NDFPRKMMNGALG--CPRILMECEVDSDCLPGCICRPNGFCG 161

[Close]

BLAST of Lcy11g004480 vs. NCBI nr
Match: KAG7014942.1 (hypothetical protein SDJN02_22573, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 97.1 bits (240), Expect = 1.1e-16
Identity = 56/102 (54.90%), Postives = 70/102 (68.63%), Query Frame = 0


Query: 17  KARDQKQIRFKSVC--EREKIMEWKKVALMAMVGMLLMASVAESSGV--VEVIELISDGG 76
           K R     R K +   +REK MEWK+ AL+AMVG+LLMA+ AES+ V   EVI+L+SDG 
Sbjct: 63  KCRSNGYERVKGIMGNKREK-MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGV 122

Query: 77  NDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLSNGYCG 115
           ND PRK+M+G  G  CPRILM C+ D DC+  C C  NG+CG
Sbjct: 123 NDFPRKMMNGALG--CPRILMECEVDSDCLPGCICRPNGFCG 161

[Close]

BLAST of Lcy11g004480 vs. NCBI nr
Match: XP_022984819.1 (trypsin inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 96.3 bits (238), Expect = 1.8e-16
Identity = 49/81 (60.49%), Postives = 61/81 (75.31%), Query Frame = 0


Query: 36  MEWKKVALMAMVGMLLMASVAESSGV--VEVIELISDGGNDLPRKIMSGRHGGICPRILM 95
           MEWK+ AL+AMVG+LLMA+ AES+ V   EVI+L+SDG ND PRK+M+G  G  CPRILM
Sbjct: 1   MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGVQG--CPRILM 60

Query: 96  PCKTDDDCMLDCRCLSNGYCG 115
            C+ D DC+  C C  NG+CG
Sbjct: 61  ECELDSDCLPGCICRPNGFCG 79

[Close]

BLAST of Lcy11g004480 vs. NCBI nr
Match: XP_023552845.1 (uncharacterized protein LOC111810370 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 94.0 bits (232), Expect = 9.1e-16
Identity = 50/86 (58.14%), Postives = 64/86 (74.42%), Query Frame = 0


Query: 31  EREKIMEWKKVALMAMVGMLLMASVAESSGV--VEVIELISDGGNDLPRKIMSGRHGGIC 90
           +REK MEWK+ AL+AMVG+LLMA+ A+S+ V   EVI+L+SDG ND PRK+M+   G  C
Sbjct: 81  KREK-MEWKRGALIAMVGLLLMAAFADSAAVHGGEVIQLVSDGVNDFPRKMMNAALG--C 140

Query: 91  PRILMPCKTDDDCMLDCRCLSNGYCG 115
           PRILM C+ D DC+  C C  NG+CG
Sbjct: 141 PRILMECEVDSDCLPGCVCRPNGFCG 163

The following BLAST results are available for this feature:

BLAST of Lcy11g004480 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P34950	1.2e-31	100.00	Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
Q43667	4.4e-13	55.22	Trypsin inhibitor 1 OS=Trichosanthes kirilowii OX=3677 PE=3 SV=1	[more]
P35628	1.4e-06	67.86	Trypsin inhibitor 4 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P01074	8.9e-06	64.29	Trypsin inhibitor 1 OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
P07853	8.9e-06	64.29	Trypsin inhibitor 4 OS=Cucurbita maxima OX=3661 PE=1 SV=2	[more]

BLAST of Lcy11g004480 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JBL8	8.9e-17	60.49	trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482991 PE=3 SV=1	[more]
A0A5D3E0W0	4.1e-14	57.32	Trypsin inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G...	[more]
A0A0A0KY34	1.2e-13	54.88	Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1	[more]
A0A0A0KT01	1.0e-12	51.25	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G000805 PE=3 SV=1	[more]
A0A4P2SJP1	3.3e-11	56.72	Trypsin inhibitor I1 OS=Trichosanthes kirilowii OX=3677 PE=2 SV=1	[more]

BLAST of Lcy11g004480 vs. NCBI nr


Match Name	E-value	Identity	Description
P34950.1	9.4e-29	100.00	RecName: Full=Trypsin inhibitor 5; AltName: Full=TGT-II; AltName: Full=Trypsin i...	[more]
KAG6576915.1	3.7e-17	54.90	hypothetical protein SDJN03_24489, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7014942.1	1.1e-16	54.90	hypothetical protein SDJN02_22573, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022984819.1	1.8e-16	60.49	trypsin inhibitor 1-like [Cucurbita maxima]	[more]
XP_023552845.1	9.1e-16	58.14	uncharacterized protein LOC111810370 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lcy11g004480 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00286	PTI_1	coord: 86..114 e-value: 1.6E-12 score: 57.6
None	No IPR available	GENE3D	4.10.75.20		coord: 53..114 e-value: 4.6E-13 score: 50.8
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 87..114 e-value: 6.1E-15 score: 54.9
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 88..107
IPR000737	Proteinase inhibitor I7, squash	CDD	cd00150	PlantTI	coord: 88..114 e-value: 2.98333E-12 score: 54.3059
IPR011052	Proteinase/amylase inhibitor domain superfamily	SUPERFAMILY	57027	Plant inhibitors of proteinases and amylases	coord: 82..114

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy11g004480.1	Lcy11g004480.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity