Lcy11g004460 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy11g004460
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	trypsin inhibitor 1-like
Location	Chr11: 4366733 .. 4367233 (+)
RNA-Seq Expression	Lcy11g004460
Synteny	Lcy11g004460

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGCAAAGATAAGAAATAAGAGAGAGAGAGAGAGAGAAAGAGAATGGAGTGGAAAAAAGCTGCTTTGGTTGCAATGGTGGGAATGTTGCTGATGGCAGCCTTTGCAGAGTCTTCCGGCGCCGGCGCCGTCGCCAATGAGGTGATTGAACTAGTTTCTGACGGAGTGGACTTCCCGGAAAATATGGTGAAGGGTGTGGTAGGCTGCCCTAGAATCCTTATGAAGTGCAAAACCAAGGAGGATTGCTTTCCAACCTGCAACTGCCTGAACGGCTATTGCGGTTGAAAACCCGCCGTGTCAGAAGACACTCCGCCGTGTTGAATGTGGTGTCGTGTTCGTGTTTTATGTCTATGTTTGTGTATTTTCTGTTTCTATGAAAATAAGGTCCATCTCTTTGATCACTCTCCTCTATGAGTGTATGTGATCAATTTGTATGAAACCCATCAAGCATTTTAATGTGTAATCTTCTATATATTTTCTCGTCAATCTAAGACATGAGCAAAT

mRNA sequence

AGCAAAGATAAGAAATAAGAGAGAGAGAGAGAGAGAAAGAGAATGGAGTGGAAAAAAGCTGCTTTGGTTGCAATGGTGGGAATGTTGCTGATGGCAGCCTTTGCAGAGTCTTCCGGCGCCGGCGCCGTCGCCAATGAGGTGATTGAACTAGTTTCTGACGGAGTGGACTTCCCGGAAAATATGGTGAAGGGTGTGGTAGGCTGCCCTAGAATCCTTATGAAGTGCAAAACCAAGGAGGATTGCTTTCCAACCTGCAACTGCCTGAACGGCTATTGCGGTTGAAAACCCGCCGTGTCAGAAGACACTCCGCCGTGTTGAATGTGGTGTCGTGTTCGTGTTTTATGTCTATGTTTGTGTATTTTCTGTTTCTATGAAAATAAGGTCCATCTCTTTGATCACTCTCCTCTATGAGTGTATGTGATCAATTTGTATGAAACCCATCAAGCATTTTAATGTGTAATCTTCTATATATTTTCTCGTCAATCTAAGACATGAGCAAAT

Coding sequence (CDS)

ATGGAGTGGAAAAAAGCTGCTTTGGTTGCAATGGTGGGAATGTTGCTGATGGCAGCCTTTGCAGAGTCTTCCGGCGCCGGCGCCGTCGCCAATGAGGTGATTGAACTAGTTTCTGACGGAGTGGACTTCCCGGAAAATATGGTGAAGGGTGTGGTAGGCTGCCCTAGAATCCTTATGAAGTGCAAAACCAAGGAGGATTGCTTTCCAACCTGCAACTGCCTGAACGGCTATTGCGGTTGA

Protein sequence

MEWKKAALVAMVGMLLMAAFAESSGAGAVANEVIELVSDGVDFPENMVKGVVGCPRILMKCKTKEDCFPTCNCLNGYCG

Homology

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BLAST of Lcy11g004460 vs. ExPASy Swiss-Prot
Match: Q43667 (Trypsin inhibitor 1 OS=Trichosanthes kirilowii OX=3677 PE=3 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 1.1e-15
Identity = 42/65 (64.62%), Postives = 50/65 (76.92%), Query Frame = 0


Query: 17 MAAFAESSGAGAVANEVIELVSDGV-DFPENMVKGVVGCPRILMKCKTKEDCFPTCNCL- 76
          MAAF ES+ AGA A+EVI+LVSDGV ++ E M++GVV CPRILM CK  +DC   C CL 
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMEGVVACPRILMPCKVNDDCLRGCKCLS 60

Query: 77 NGYCG 80
          NGYCG
Sbjct: 61 NGYCG 65

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BLAST of Lcy11g004460 vs. ExPASy Swiss-Prot
Match: P34950 (Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 7.0e-10
Identity = 38/67 (56.72%), Postives = 43/67 (64.18%), Query Frame = 0


Query: 17 MAAFAESSGAGAVANEVIELVSD-GVDFPENMVKGVVG--CPRILMKCKTKEDCFPTCNC 76
          MA+ AESSG      EVIEL+SD G D P  ++ G  G  CPRILM CKT +DC   C C
Sbjct: 1  MASVAESSG----VVEVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRC 60

Query: 77 L-NGYCG 80
          L NGYCG
Sbjct: 61 LSNGYCG 63

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BLAST of Lcy11g004460 vs. ExPASy Swiss-Prot
Match: P84452 (Trypsin inhibitor 5 OS=Sechium edule OX=184140 PE=1 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 6.8e-05
Identity = 19/27 (70.37%), Postives = 21/27 (77.78%), Query Frame = 0


Query: 54 CPRILMKCKTKEDCFPTCNCL-NGYCG 80
          CPRILMKCK   DCFPTC C  +G+CG
Sbjct: 1  CPRILMKCKLDTDCFPTCTCRPSGFCG 27

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BLAST of Lcy11g004460 vs. ExPASy TrEMBL
Match: A0A6J1JBL8 (trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482991 PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.6e-17
Identity = 52/81 (64.20%), Postives = 60/81 (74.07%), Query Frame = 0


Query: 1  MEWKKAALVAMVGMLLMAAFAESSGAGAVANEVIELVSDGV-DFPENMVKGVVGCPRILM 60
          MEWK+ AL+AMVG+LLMAAFAES  A     EVI+LVSDGV DFP  M+ GV GCPRILM
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAES--AAVHGGEVIQLVSDGVNDFPRKMMNGVQGCPRILM 60

Query: 61 KCKTKEDCFPTCNCL-NGYCG 80
          +C+   DC P C C  NG+CG
Sbjct: 61 ECELDSDCLPGCICRPNGFCG 79

[Close]

BLAST of Lcy11g004460 vs. ExPASy TrEMBL
Match: A0A0A0KY34 (Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 2.3e-16
Identity = 52/82 (63.41%), Postives = 60/82 (73.17%), Query Frame = 0


Query: 1  MEWKKAALVAMVGMLLMAAFAESSGAGAVANEVIELVSDGV-DFPENMVKGVV-GCPRIL 60
          MEWKK ALVAM+GMLLMA F ES G G V  E+I+LVSDGV ++ +N+ K    GCPRIL
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLG-VDEEIIQLVSDGVNEYSQNIGKDTAPGCPRIL 60

Query: 61 MKCKTKEDCFPTCNCL-NGYCG 80
          MKCKT  DC+P C C   GYCG
Sbjct: 61 MKCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of Lcy11g004460 vs. ExPASy TrEMBL
Match: A0A5D3E0W0 (Trypsin inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00600 PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.2e-15
Identity = 52/80 (65.00%), Postives = 55/80 (68.75%), Query Frame = 0


Query: 1  MEWKKAALVAMVGMLLMAAFAESSGAGAVANEVIELVSDGVDFPENMVKGVVGCPRILMK 60
          MEWKK ALVAM+GMLLMA F ES G G V  E+I+LVSDGV    N   G  GCPRILMK
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGFG-VDEEIIQLVSDGV----NEYSGHPGCPRILMK 60

Query: 61 CKTKEDCFPTCNC-LNGYCG 80
          CKT  DC   C C  NGYCG
Sbjct: 61 CKTDRDCLAGCTCKRNGYCG 75

[Close]

BLAST of Lcy11g004460 vs. ExPASy TrEMBL
Match: A0A4P2SJP1 (Trypsin inhibitor I1 OS=Trichosanthes kirilowii OX=3677 PE=2 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 2.9e-14
Identity = 44/65 (67.69%), Postives = 51/65 (78.46%), Query Frame = 0


Query: 17 MAAFAESSGAGAVANEVIELVSDGV-DFPENMVKGVVGCPRILMKCKTKEDCFPTCNCL- 76
          MAAF ES+ AGA A+EVI+LVSDGV ++ E M+KGVVGCPRILM CK  +DC   C CL 
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMKGVVGCPRILMPCKVNDDCLRGCKCLS 60

Query: 77 NGYCG 80
          NGYCG
Sbjct: 61 NGYCG 65

[Close]

BLAST of Lcy11g004460 vs. ExPASy TrEMBL
Match: A0A4P2SLY2 (Trypsin inhibitor I2 OS=Trichosanthes kirilowii OX=3677 PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.1e-13
Identity = 42/65 (64.62%), Postives = 51/65 (78.46%), Query Frame = 0


Query: 17 MAAFAESSGAGAVANEVIELVSDGV-DFPENMVKGVVGCPRILMKCKTKEDCFPTCNCL- 76
          MAAF ES+ AGA A+EVI+LVSDGV ++ E M++GVVGCPR+LM CK  +DC   C CL 
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMEGVVGCPRVLMPCKVNDDCLRGCKCLS 60

Query: 77 NGYCG 80
          NGYCG
Sbjct: 61 NGYCG 65

[Close]

BLAST of Lcy11g004460 vs. NCBI nr
Match: KAG6576915.1 (hypothetical protein SDJN03_24489, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 98.2 bits (243), Expect = 3.3e-17
Identity = 51/81 (62.96%), Postives = 60/81 (74.07%), Query Frame = 0


Query: 1   MEWKKAALVAMVGMLLMAAFAESSGAGAVANEVIELVSDGV-DFPENMVKGVVGCPRILM 60
           MEWK+ AL+AMVG+LLMAAFAES  A     EVI+LVSDGV DFP  M+ G +GCPRILM
Sbjct: 83  MEWKRGALIAMVGLLLMAAFAES--AAVHGGEVIQLVSDGVNDFPRKMMNGALGCPRILM 142

Query: 61  KCKTKEDCFPTCNCL-NGYCG 80
           +C+   DC P C C  NG+CG
Sbjct: 143 ECEVDSDCLPGCICRPNGFCG 161

[Close]

BLAST of Lcy11g004460 vs. NCBI nr
Match: KAG7014942.1 (hypothetical protein SDJN02_22573, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 98.2 bits (243), Expect = 3.3e-17
Identity = 51/81 (62.96%), Postives = 60/81 (74.07%), Query Frame = 0


Query: 1   MEWKKAALVAMVGMLLMAAFAESSGAGAVANEVIELVSDGV-DFPENMVKGVVGCPRILM 60
           MEWK+ AL+AMVG+LLMAAFAES  A     EVI+LVSDGV DFP  M+ G +GCPRILM
Sbjct: 83  MEWKRGALIAMVGLLLMAAFAES--AAVHGGEVIQLVSDGVNDFPRKMMNGALGCPRILM 142

Query: 61  KCKTKEDCFPTCNCL-NGYCG 80
           +C+   DC P C C  NG+CG
Sbjct: 143 ECEVDSDCLPGCICRPNGFCG 161

[Close]

BLAST of Lcy11g004460 vs. NCBI nr
Match: XP_022984819.1 (trypsin inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 98.2 bits (243), Expect = 3.3e-17
Identity = 52/81 (64.20%), Postives = 60/81 (74.07%), Query Frame = 0


Query: 1  MEWKKAALVAMVGMLLMAAFAESSGAGAVANEVIELVSDGV-DFPENMVKGVVGCPRILM 60
          MEWK+ AL+AMVG+LLMAAFAES  A     EVI+LVSDGV DFP  M+ GV GCPRILM
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAES--AAVHGGEVIQLVSDGVNDFPRKMMNGVQGCPRILM 60

Query: 61 KCKTKEDCFPTCNCL-NGYCG 80
          +C+   DC P C C  NG+CG
Sbjct: 61 ECELDSDCLPGCICRPNGFCG 79

[Close]

BLAST of Lcy11g004460 vs. NCBI nr
Match: XP_023552845.1 (uncharacterized protein LOC111810370 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 94.7 bits (234), Expect = 3.7e-16
Identity = 49/81 (60.49%), Postives = 59/81 (72.84%), Query Frame = 0


Query: 1   MEWKKAALVAMVGMLLMAAFAESSGAGAVANEVIELVSDGV-DFPENMVKGVVGCPRILM 60
           MEWK+ AL+AMVG+LLMAAFA+S  A     EVI+LVSDGV DFP  M+   +GCPRILM
Sbjct: 85  MEWKRGALIAMVGLLLMAAFADS--AAVHGGEVIQLVSDGVNDFPRKMMNAALGCPRILM 144

Query: 61  KCKTKEDCFPTCNCL-NGYCG 80
           +C+   DC P C C  NG+CG
Sbjct: 145 ECEVDSDCLPGCVCRPNGFCG 163

[Close]

BLAST of Lcy11g004460 vs. NCBI nr
Match: KGN52761.1 (hypothetical protein Csa_014657 [Cucumis sativus])

HSP 1 Score: 94.4 bits (233), Expect = 4.8e-16
Identity = 52/82 (63.41%), Postives = 60/82 (73.17%), Query Frame = 0


Query: 1  MEWKKAALVAMVGMLLMAAFAESSGAGAVANEVIELVSDGV-DFPENMVKGVV-GCPRIL 60
          MEWKK ALVAM+GMLLMA F ES G G V  E+I+LVSDGV ++ +N+ K    GCPRIL
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLG-VDEEIIQLVSDGVNEYSQNIGKDTAPGCPRIL 60

Query: 61 MKCKTKEDCFPTCNCL-NGYCG 80
          MKCKT  DC+P C C   GYCG
Sbjct: 61 MKCKTDSDCYPGCTCKPMGYCG 81

The following BLAST results are available for this feature:

BLAST of Lcy11g004460 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q43667	1.1e-15	64.62	Trypsin inhibitor 1 OS=Trichosanthes kirilowii OX=3677 PE=3 SV=1	[more]
P34950	7.0e-10	56.72	Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P84452	6.8e-05	70.37	Trypsin inhibitor 5 OS=Sechium edule OX=184140 PE=1 SV=1	[more]

BLAST of Lcy11g004460 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JBL8	1.6e-17	64.20	trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482991 PE=3 SV=1	[more]
A0A0A0KY34	2.3e-16	63.41	Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1	[more]
A0A5D3E0W0	1.2e-15	65.00	Trypsin inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G...	[more]
A0A4P2SJP1	2.9e-14	67.69	Trypsin inhibitor I1 OS=Trichosanthes kirilowii OX=3677 PE=2 SV=1	[more]
A0A4P2SLY2	1.1e-13	64.62	Trypsin inhibitor I2 OS=Trichosanthes kirilowii OX=3677 PE=2 SV=1	[more]

BLAST of Lcy11g004460 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6576915.1	3.3e-17	62.96	hypothetical protein SDJN03_24489, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7014942.1	3.3e-17	62.96	hypothetical protein SDJN02_22573, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022984819.1	3.3e-17	64.20	trypsin inhibitor 1-like [Cucurbita maxima]	[more]
XP_023552845.1	3.7e-16	60.49	uncharacterized protein LOC111810370 [Cucurbita pepo subsp. pepo]	[more]
KGN52761.1	4.8e-16	63.41	hypothetical protein Csa_014657 [Cucumis sativus]	[more]

BLAST of Lcy11g004460 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00286	PTI_1	coord: 52..79 e-value: 3.1E-7 score: 40.0
None	No IPR available	GENE3D	4.10.75.20		coord: 22..79 e-value: 2.2E-9 score: 39.0
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 54..79 e-value: 2.4E-10 score: 40.3
IPR000737	Proteinase inhibitor I7, squash	CDD	cd00150	PlantTI	coord: 54..79 e-value: 5.05764E-7 score: 40.0535
IPR011052	Proteinase/amylase inhibitor domain superfamily	SUPERFAMILY	57027	Plant inhibitors of proteinases and amylases	coord: 53..79

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy11g004460.1	Lcy11g004460.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010466	negative regulation of peptidase activity
cellular_component	GO:0110165	cellular anatomical entity
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity