Homology
BLAST of Lcy11g001550 vs. ExPASy Swiss-Prot
Match:
Q2QYH7 (Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 SV=1)
HSP 1 Score: 329.3 bits (843), Expect = 7.7e-89
Identity = 183/511 (35.81%), Postives = 277/511 (54.21%), Query Frame = 0
Query: 9 MVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKRTMKAAAAINGGSFASS 68
++LF V I W+ P QKL+ G GP PSF GNI EM+R + A + + A S
Sbjct: 20 ILLFSYVYIILWLRPERLRQKLRSQGVRGPKPSFLFGNIPEMRRIQQLAKSAHEQE-AGS 79
Query: 69 SNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGWGKP 128
++ S + + +FP+F W YG ++Y G+ L + DP VK+++ GKP
Sbjct: 80 TDMFSSNYVATLFPYFLHWSRVYGSIYLYSTGSIQVLNVTDPNMVKELANC-KSLDLGKP 139
Query: 129 AVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKWSSL 188
+ +R + G G++ + GD W+ R ++ P +K M M+E+ MLN W +
Sbjct: 140 CYLQKERGALLGMGILTSNGDLWVHQRKVIAPELFMERVKGMVNLMMEAAMSMLNSWKNE 199
Query: 189 V--NSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGVP 248
V EI V+ + + D+I++A FG S G++ F +R LQ + + S +GVP
Sbjct: 200 VEDRGGSAEIVVDEFLRTFSADVISRACFGSSFSEGKEIFIKIRQLQKAMAKQSMLIGVP 259
Query: 249 FSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGGK 308
S +L L + I L L++ + + S + DLL +I+ GS G
Sbjct: 260 GS-RYLPTRSNRGIWNLDSSIRTLILNISKKYEHDSSTSVNKDLLHSIIQ----GSKDGP 319
Query: 309 MRLTTRE--LVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEID 368
T E +VD CK +F GHETT+ +W L+LLA+ EWQ+ R E ++ + +D
Sbjct: 320 FASCTPEDFIVDNCKNIYFAGHETTSTTAAWCLMLLASHHEWQSRARVESLDICQGRPLD 379
Query: 369 FAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPT 428
F +L L+K+ V E LRLYP A + R+A D+K+G + IPKGTNIWI + H DP+
Sbjct: 380 FDILRKLKKLTMVIQETLRLYPPASFVAREALNDMKLGGIDIPKGTNIWIPIAMAHRDPS 439
Query: 429 LWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRF 488
+WG ++F P+RF + I G C Y+PFG G R C G+NL+ +E K+VL+L+LS+F
Sbjct: 440 VWGPSADKFDPDRF-ANGIAGACKPPHMYMPFGVGVRTCAGQNLAMVELKVVLSLLLSKF 499
Query: 489 SLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 516
LSP+Y H PA L++ P G+PLI + L
Sbjct: 500 EFKLSPNYVHCPAFRLTIEPGKGVPLIFREL 522
BLAST of Lcy11g001550 vs. ExPASy Swiss-Prot
Match:
Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)
HSP 1 Score: 327.4 bits (838), Expect = 2.9e-88
Identity = 184/520 (35.38%), Postives = 284/520 (54.62%), Query Frame = 0
Query: 4 IRVSIMVLFVKV----VISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKRTMKAAAA 63
+ V +M L ++V + +++ P + ++R G GP P GNI ++ + + +A
Sbjct: 10 VLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDISKMLSHSA- 69
Query: 64 INGGSFASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKI--- 123
++ +++ H+I + PH+ W YGK+F+ W GTEP L + + + +K++
Sbjct: 70 ------SNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELLTK 129
Query: 124 SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVE 183
V GK W + + + G GL+M G+ W RH+ PAF LK K MVE
Sbjct: 130 HNPVTGKSW----LQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVE 189
Query: 184 STTQMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTL 243
T M + L E+++ E+ DII++ FG S G++ F+ L LQ
Sbjct: 190 CTKMMAER---LRKEVGEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLC 249
Query: 244 FQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIE 303
Q + ++ P S FL K L E++RL + +I RK S E SS L+
Sbjct: 250 AQATRHLCFPGS-RFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLG 309
Query: 304 SQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKE 363
N K L + ++DECKTFFF GHETT+L ++WTL+LLA P WQ+ +R+E+++
Sbjct: 310 LLLNQMDSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQ 369
Query: 364 VIGDKEI-DFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWID 423
V G + LS L + V NE LRLYP A + R A DIK+GD+ IPKG +IWI
Sbjct: 370 VCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIP 429
Query: 424 VVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKI 483
V+++HH LWGED NEF PERF S H ++PF G R CIG+ + ME KI
Sbjct: 430 VLAIHHSNELWGEDANEFNPERFTTRSF-ASSRH---FMPFAAGPRNCIGQTFAMMEAKI 489
Query: 484 VLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 516
+L +++S+FS ++S +Y H+P ++L+++P +G+ L++KPL
Sbjct: 490 ILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPL 510
BLAST of Lcy11g001550 vs. ExPASy Swiss-Prot
Match:
Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)
HSP 1 Score: 326.2 bits (835), Expect = 6.5e-88
Identity = 184/527 (34.91%), Postives = 284/527 (53.89%), Query Frame = 0
Query: 7 SIMVLFVKVVI--------SWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKRTMKAAA 66
S++V+FV ++ +W+ P + +++ G GP P GNI E+ +
Sbjct: 8 SLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEI-------S 67
Query: 67 AINGGSFASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKI-- 126
A+ S + +++ HDI + PH+ W YGK+F+ W GT+P L + + + +K++
Sbjct: 68 AMVSQSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLM 127
Query: 127 -SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMV 186
V G+ W + +N G GL+M G DW RH+ PAF LK + MV
Sbjct: 128 KHNGVSGRSWLQQQGTKN----FIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMV 187
Query: 187 ESTTQMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFT 246
E T++++ + V E+++ E+ DII++ FG S G++ FN L LQ
Sbjct: 188 ECTSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRR 247
Query: 247 LFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSS----DLL 306
Q + ++ P S FL K L E++RL + +I R+ E SS DLL
Sbjct: 248 CAQATRHLCFPGS-RFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLL 307
Query: 307 SRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILR 366
L+ + ++DECKTFFF GHETTAL ++WT +LLA P WQ +R
Sbjct: 308 GLLLNEMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVR 367
Query: 367 EEIKEVIGDKEI-DFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGT 426
EE++EV G + LS L + V NE LRLYP A + R A D+K+GD+TIPKG
Sbjct: 368 EEVREVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGL 427
Query: 427 NIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMG--YLPFGFGGRMCIGRNL 486
+IWI V+++HH LWG+D N+F PERF GG G ++PF G R CIG+
Sbjct: 428 SIWIPVLAIHHSEELWGKDANQFNPERF------GGRPFASGRHFIPFAAGPRNCIGQQF 487
Query: 487 SSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 516
+ ME KI+L ++S+F+ ++S +Y H+P ++L+++P +G+ +I+KPL
Sbjct: 488 ALMEAKIILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516
BLAST of Lcy11g001550 vs. ExPASy Swiss-Prot
Match:
B6SSW8 (Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1)
HSP 1 Score: 310.1 bits (793), Expect = 4.8e-83
Identity = 176/504 (34.92%), Postives = 268/504 (53.17%), Query Frame = 0
Query: 20 WILPILAHQKLKRNGFEGPSPSFPLGNIAEMKRTMKAAAAINGGSFASSS--NNVSHDIH 79
W++P K + GP PSFP GN+A+M+ AA A + S S + HD
Sbjct: 32 WLVPQKVLAKFEDQKIGGPRPSFPYGNLADMREAAAAAKAARASARRSGSGGGGIVHDYR 91
Query: 80 SIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGWGKPAVFRNDREP 139
V P++ +W+ YG F Y +G FL+++ P V+ I+ V GK + + EP
Sbjct: 92 PAVLPYYEKWRKEYGPIFTYSMGNVVFLHVSRPDVVRDINLCV-SLDLGKSSYLKATHEP 151
Query: 140 MFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKWSSLV--NSAQPE 199
+FG G++ + G+ W+ R I+ P F +K M MV+S +L W V N +
Sbjct: 152 LFGGGILKSNGEAWLHQRKIIAPEFFLDKVKGMVDLMVDSAQPLLMSWEERVDRNGGITD 211
Query: 200 IDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGVPFSGLFLCPA 259
I ++ +I A + D+I++ FG S G++ F +R LQ + + V +GL P+
Sbjct: 212 IKIDDDIRAYSADVISRTCFGSSYIKGKEIFMKIRELQQAV--SKPNVLAEMTGLRFFPS 271
Query: 260 -RTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGGKMRLTTREL 319
R A L ++ +L L ++ +E+ +LLS ++ S G + +
Sbjct: 272 MRNKQAWELHKQVRKLILEIV------KESGEDRNLLSAILHSASTSRVG--IAEAENFI 331
Query: 320 VDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEIDFAMLSGLRKM 379
VD CK+ +F GHE+TA+ +W L+LL PEWQN +REE+ EV + +D L ++ +
Sbjct: 332 VDNCKSIYFAGHESTAVTAAWCLMLLGLHPEWQNRVREEVHEVCRGQPVDSRSLQKMKNL 391
Query: 380 GWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTLWGEDVNEFK 439
V E LRLYP+ + RQA ++K+G V IPKG NI+I V +MH DP LWG DV EF
Sbjct: 392 TMVIQETLRLYPAGAFVSRQALQELKLGGVHIPKGVNIYIPVSTMHLDPELWGPDVKEFN 451
Query: 440 PERFE--KDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFSLSLSPSY 499
PERF + +H YLPFG G R C+G+ + E KI+++LI+S+F L LSP Y
Sbjct: 452 PERFSDVRPQLH-------SYLPFGAGARTCLGQGFAMAELKILISLIVSKFVLKLSPHY 511
Query: 500 SHSPAILLSLRPAHGLPLIVKPLQ 517
HSP + L + P G+ L + +Q
Sbjct: 512 QHSPTLKLIVEPELGVDLTLTKVQ 517
BLAST of Lcy11g001550 vs. ExPASy Swiss-Prot
Match:
Q6NKZ8 (Cytochrome P450 714A2 OS=Arabidopsis thaliana OX=3702 GN=CYP714A2 PE=2 SV=1)
HSP 1 Score: 307.0 bits (785), Expect = 4.1e-82
Identity = 173/515 (33.59%), Postives = 273/515 (53.01%), Query Frame = 0
Query: 11 LFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKRTMKAAAAINGGSFASSSN 70
++ + V+ W + + LK G +GP PS GN++EM+R A S N
Sbjct: 27 VYGRAVVEQWRM----RRSLKLQGVKGPPPSIFNGNVSEMQRIQSEA------KHCSGDN 86
Query: 71 NVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGWGKPAV 130
+SHD S +FPHF W+ YG+ + Y G + LYI P+ VK++S+ G+
Sbjct: 87 IISHDYSSSLFPHFDHWRKQYGRIYTYSTGLKQHLYINHPEMVKELSQ-TNTLNLGRITH 146
Query: 131 FRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKWSSLVN 190
P+ G+G++ + G W R I+ F +K M MVES MLNKW +V
Sbjct: 147 ITKRLNPILGNGIITSNGPHWAHQRRIIAYEFTHDKIKGMVGLMVESAMPMLNKWEEMVK 206
Query: 191 SAQP---EIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGVPF 250
+I V+ ++ + D+IA+A FG S G+ F+ +R L + + S V F
Sbjct: 207 RGGEMGCDIRVDEDLKDVSADVIAKACFGSSFSKGKAIFSMIRDLLTAITKRS--VLFRF 266
Query: 251 SGLFLCPARTLAAKRLG-AEIDRLFLSV-------ISERKQSEENSSSSDLLSRLIESQR 310
+G +K+ G +ID L + + + ER+ +++ DL+ ++E
Sbjct: 267 NGF---TDMVFGSKKHGDVDIDALEMELESSIWETVKEREIECKDTHKKDLMQLILEGAM 326
Query: 311 NGSGGGKMRLTT--RELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEV 370
G + R +VD CK+ +F GH++TA+++SW L+LLA P WQ +R+EI
Sbjct: 327 RSCDGNLWDKSAYRRFVVDNCKSIYFAGHDSTAVSVSWCLMLLALNPSWQVKIRDEILSS 386
Query: 371 IGDKEIDFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVV 430
+ D + L+ + V E +RLYP AP + R+A DI++GD+ +PKG IW +
Sbjct: 387 CKNGIPDAESIPNLKTVTMVIQETMRLYPPAPIVGREASKDIRLGDLVVPKGVCIWTLIP 446
Query: 431 SMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVL 490
++H DP +WG D N+FKPERF + I C + Y+PFG G R C+G+N ME K+++
Sbjct: 447 ALHRDPEIWGPDANDFKPERF-SEGISKACKYPQSYIPFGLGPRTCVGKNFGMMEVKVLV 506
Query: 491 TLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIV 513
+LI+S+FS +LSP+Y HSP+ L + P HG+ + V
Sbjct: 507 SLIVSKFSFTLSPTYQHSPSHKLLVEPQHGVVIRV 524
BLAST of Lcy11g001550 vs. ExPASy TrEMBL
Match:
A0A6J1G224 (cytochrome P450 714C2-like OS=Cucurbita moschata OX=3662 GN=LOC111449972 PE=3 SV=1)
HSP 1 Score: 886.3 bits (2289), Expect = 6.0e-254
Identity = 434/512 (84.77%), Postives = 468/512 (91.41%), Query Frame = 0
Query: 6 VSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKRTMKAAAAINGGSF 65
V +MVLFVKVV+SWW+LP+LAHQKLKRNGF GP PSFPLGNIAEMKR KA+AAING +
Sbjct: 14 VLVMVLFVKVVVSWWVLPVLAHQKLKRNGFRGPPPSFPLGNIAEMKRIRKASAAINGDAH 73
Query: 66 ASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGW 125
+ S+ VSHDIHS VFPHFAQWQASYGKKF+YWLGTEPFLYIADP F+KKISEAVM KGW
Sbjct: 74 SFPSSKVSHDIHSTVFPHFAQWQASYGKKFIYWLGTEPFLYIADPHFMKKISEAVMAKGW 133
Query: 126 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKW 185
GKPAVFRNDR PMFGDGLVMTEGDDW+RHRHILTPAFNPANLKAMTKFMVESTT+MLNKW
Sbjct: 134 GKPAVFRNDRRPMFGDGLVMTEGDDWVRHRHILTPAFNPANLKAMTKFMVESTTKMLNKW 193
Query: 186 SSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV 245
SSLVNSA EIDVEREITATAGDIIA+ASFGI N GGRDAF+ LRALQFTLFQNSTYVGV
Sbjct: 194 SSLVNSAGGEIDVEREITATAGDIIAEASFGICNDGGRDAFDYLRALQFTLFQNSTYVGV 253
Query: 246 PFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGG 305
PFS LF CP RTL+AKRLGA IDRLFLS++S+RK S++ SSSDLLSRLIE +++ GG
Sbjct: 254 PFSSLFFCPGRTLSAKRLGAAIDRLFLSLVSKRKLSQD--SSSDLLSRLIEGEKSTGDGG 313
Query: 306 KMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEIDF 365
KM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+ILREEIKEVIGDKEIDF
Sbjct: 314 KMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGDKEIDF 373
Query: 366 AMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTL 425
MLSGL+KMG VF+EVLRLYPSAPNIQRQAKGDI VG +TIPKGTNIWID+V+MH DP L
Sbjct: 374 NMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIDVGSMTIPKGTNIWIDIVAMHRDPDL 433
Query: 426 WGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFS 485
WG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG+NLSSMEYKIVLTLILSRFS
Sbjct: 434 WGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKNLSSMEYKIVLTLILSRFS 493
Query: 486 LSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 518
LSLSP+Y+HSPA LLSLRPAHGLPLIV PL P
Sbjct: 494 LSLSPNYAHSPATLLSLRPAHGLPLIVTPLHP 523
BLAST of Lcy11g001550 vs. ExPASy TrEMBL
Match:
A0A6J1HYD5 (cytochrome P450 714C2-like OS=Cucurbita maxima OX=3661 GN=LOC111467387 PE=3 SV=1)
HSP 1 Score: 880.9 bits (2275), Expect = 2.5e-252
Identity = 435/512 (84.96%), Postives = 467/512 (91.21%), Query Frame = 0
Query: 6 VSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKRTMKAAAAINGGSF 65
V IMVLFVKVV+SWW+LP+LAHQKLKRNGF GP P+FPLGNIAEMKR KA+AAING S
Sbjct: 14 VLIMVLFVKVVVSWWVLPVLAHQKLKRNGFRGPPPTFPLGNIAEMKRIRKASAAINGDSH 73
Query: 66 ASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGW 125
+ S+ VSHDIHS VFPHFAQWQASYGKKFVYWLGTEPFLYIADP F+K+IS AVM KGW
Sbjct: 74 SFPSSKVSHDIHSTVFPHFAQWQASYGKKFVYWLGTEPFLYIADPHFMKQISVAVMAKGW 133
Query: 126 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKW 185
GKPAVFRNDR PMFGDGLVMTEGD W+RHRHILTPAFNPANLKAMTKFMVESTT+MLN+W
Sbjct: 134 GKPAVFRNDRRPMFGDGLVMTEGDVWVRHRHILTPAFNPANLKAMTKFMVESTTKMLNEW 193
Query: 186 SSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV 245
SSLVNSA EIDVEREITATAGDIIA+ASFGI NGGGRDAF+ LRALQFTLFQNSTYVGV
Sbjct: 194 SSLVNSASGEIDVEREITATAGDIIAKASFGICNGGGRDAFDYLRALQFTLFQNSTYVGV 253
Query: 246 PFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGG 305
PFS LF CP RTLAAKRLGA IDRLFLS++SERK S+ +SSSDLLSRLIE Q++ G
Sbjct: 254 PFSSLFFCPGRTLAAKRLGAAIDRLFLSLVSERKLSQ--NSSSDLLSRLIEGQKSTGDVG 313
Query: 306 KMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEIDF 365
KM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+ILREEIKEVIGDKEIDF
Sbjct: 314 KMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGDKEIDF 373
Query: 366 AMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTL 425
MLSGL+KMG VF+EVLRLYPSAPNIQRQAKGDI+VG +TIPKGTNIWID+V+MH DP L
Sbjct: 374 NMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIEVGSMTIPKGTNIWIDIVAMHRDPDL 433
Query: 426 WGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFS 485
WG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG++LSSMEYKIVLTLILSRFS
Sbjct: 434 WGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKSLSSMEYKIVLTLILSRFS 493
Query: 486 LSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 518
LSLSP+YSHSPA LLSLRPAHGLPLIV PL P
Sbjct: 494 LSLSPNYSHSPATLLSLRPAHGLPLIVTPLHP 523
BLAST of Lcy11g001550 vs. ExPASy TrEMBL
Match:
A0A6J1CIQ8 (cytokinin hydroxylase OS=Momordica charantia OX=3673 GN=LOC111011538 PE=3 SV=1)
HSP 1 Score: 875.5 bits (2261), Expect = 1.1e-250
Identity = 436/524 (83.21%), Postives = 476/524 (90.84%), Query Frame = 0
Query: 4 IRVSIMVLFVKVVISWWILPILAHQKLKRNGFE-GPSPSFPLGNIAEMKRTMK------A 63
IRVS++VL V+VV SWWILP+LAH+KLKRNGF GP P FPLGNI E+K TMK A
Sbjct: 12 IRVSLVVLLVRVVFSWWILPVLAHRKLKRNGFNGGPPPRFPLGNITEIKNTMKKAAASAA 71
Query: 64 AAAINGGSFA-SSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKK 123
AAAINGG+ A SSS+N+SHDIHS VFP+FAQWQ SYGKKFVYWLGTEPFLYIA+PQFVK
Sbjct: 72 AAAINGGAGAFSSSSNISHDIHSTVFPYFAQWQTSYGKKFVYWLGTEPFLYIAEPQFVKN 131
Query: 124 ISEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMV 183
+SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMT+ MV
Sbjct: 132 MSEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTRLMV 191
Query: 184 ESTTQMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFT 243
STT+ML++W+ ++S PE+DVEREITATAGDIIA+ASFGI + GRDAFN LR LQFT
Sbjct: 192 GSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIAKASFGICDRSGRDAFNKLRDLQFT 251
Query: 244 LFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLI 303
LF+NS+YVGVPFS +FL P RTL AKRLG+EIDRLFLS+ISERKQS + +S+SDLLSRLI
Sbjct: 252 LFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRLFLSIISERKQSPD-ASASDLLSRLI 311
Query: 304 ESQRNGS--GGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREE 363
ESQRN S GK LTTRELVDECKTFFFGGHETTALAISWT+LLLATRPEWQ ILREE
Sbjct: 312 ESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHETTALAISWTMLLLATRPEWQTILREE 371
Query: 364 IKEVIGDKEIDFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIW 423
IKEVIGD+EIDFAMLS L+KMGWVFNEVLRLYPSAPNIQRQAKGDIKVGD TIPKGTNIW
Sbjct: 372 IKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDTTIPKGTNIW 431
Query: 424 IDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEY 483
ID+V+MHHDP+LWG+DVNEFKPERFEKDSIHGGC HKMGYLPFGFGGRMCIGRNLSSMEY
Sbjct: 432 IDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCKHKMGYLPFGFGGRMCIGRNLSSMEY 491
Query: 484 KIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 518
KIVLTLI+SRFSLS+SPSYSHSPAILLSLRPAHGLPLI+KPL P
Sbjct: 492 KIVLTLIISRFSLSISPSYSHSPAILLSLRPAHGLPLILKPLHP 534
BLAST of Lcy11g001550 vs. ExPASy TrEMBL
Match:
A0A1S3AXX2 (cytokinin hydroxylase OS=Cucumis melo OX=3656 GN=LOC103484084 PE=3 SV=1)
HSP 1 Score: 854.4 bits (2206), Expect = 2.5e-244
Identity = 423/517 (81.82%), Postives = 460/517 (88.97%), Query Frame = 0
Query: 2 WAIRVSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKRTMKAAAAIN 61
W IRVSIMVLF KVVIS WILPILAH+KLKRNGFEGPSPSFPLGNI EMK+ MKAAAA
Sbjct: 13 WMIRVSIMVLFGKVVISCWILPILAHRKLKRNGFEGPSPSFPLGNITEMKKIMKAAAAAT 72
Query: 62 GGSFASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVM 121
+ SSSNN+SHDIHS +FPHFAQWQ SYGKKFVYWLGTEPFLYIADPQ VKKISEAV+
Sbjct: 73 AVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAVL 132
Query: 122 GKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQM 181
GK WGKPAVFRNDREPMFGDGLVMTEGD+WIRHRHILTPAFNP NLKAMTKFMVESTT+M
Sbjct: 133 GKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKAMTKFMVESTTKM 192
Query: 182 LNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISN-GGGRDAFNSLRALQFTLFQNS 241
L +W SLV S Q EI+VER+ITATAG+IIA+ASFGISN GGG +A N+LRALQ TLF+NS
Sbjct: 193 LEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNNLRALQLTLFKNS 252
Query: 242 TYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRN 301
+YVGVPFS LFL PARTLAAKRLGA+IDRLFLS+IS RK S S SDLLSRLI S+
Sbjct: 253 SYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYSDLLSRLIGSEYT 312
Query: 302 GSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGD 361
G L+ RE+VDECKTFFFGGHETTALAISWTLLLLAT P+WQ ILREEIKEV+GD
Sbjct: 313 GGEA----LSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQRILREEIKEVVGD 372
Query: 362 KEIDFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMH 421
K+IDF+MLS L+KMGWV+NEVLRLYPSAPNIQRQAKGDI +GD+ IPKGTNIWIDVV+MH
Sbjct: 373 KDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPKGTNIWIDVVAMH 432
Query: 422 HDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLI 481
HDPTLWG+DVN+F P RF+ D+IHGGC HKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLI
Sbjct: 433 HDPTLWGDDVNDFNPSRFQHDTIHGGCYHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLI 492
Query: 482 LSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 518
LS FSLSLSPSYSHSPA LLSLRPAHG+PLI+ PLQP
Sbjct: 493 LSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 525
BLAST of Lcy11g001550 vs. ExPASy TrEMBL
Match:
A0A5D3DI76 (Cytokinin hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G00640 PE=3 SV=1)
HSP 1 Score: 775.0 bits (2000), Expect = 1.9e-220
Identity = 381/469 (81.24%), Postives = 417/469 (88.91%), Query Frame = 0
Query: 50 MKRTMKAAAAINGGSFASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIAD 109
MK+ MKAAAA + SSSNN+SHDIHS +FPHFAQWQ SYGKKFVYWLGTEPFLYIAD
Sbjct: 1 MKKIMKAAAAATAVTNGSSSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIAD 60
Query: 110 PQFVKKISEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKA 169
PQ VKKISEAV+GK WGKPAVFRNDREPMFGDGLVMTEGD+WIRHRHILTPAFNP NLKA
Sbjct: 61 PQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFNPTNLKA 120
Query: 170 MTKFMVESTTQMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISN-GGGRDAFNS 229
MTKFMVESTT+ML +W SLV S Q EI+VER+ITATAG+IIA+ASFGISN GGG +A N+
Sbjct: 121 MTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIAKASFGISNCGGGGEALNN 180
Query: 230 LRALQFTLFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSS 289
LRALQ TLF+NS+YVGVPFS LFL PARTLAAKRLGA+IDRLFLS+IS RK S S S
Sbjct: 181 LRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRLFLSIISNRKMSGSGSGYS 240
Query: 290 DLLSRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQN 349
DLLSRLI S+ G L+ RE+VDECKTFFFGGHETTALAISWTLLLLAT P+WQ
Sbjct: 241 DLLSRLIGSEYTGGEA----LSPREVVDECKTFFFGGHETTALAISWTLLLLATSPDWQR 300
Query: 350 ILREEIKEVIGDKEIDFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPK 409
ILREEIKEV+GDK+IDF+MLS L+KMGWV+NEVLRLYPSAPNIQRQAKGDI +GD+ IPK
Sbjct: 301 ILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDIKIPK 360
Query: 410 GTNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNL 469
GTNIWIDVV+MHHDPTLWG+DVN+F P RF+ D+IHGGCNHKMGYLPFGFGGRMCIGRNL
Sbjct: 361 GTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNL 420
Query: 470 SSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 518
SSMEYKIVLTLILS FSLSLSPSYSHSPA LLSLRPAHG+PLI+ PLQP
Sbjct: 421 SSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLQP 465
BLAST of Lcy11g001550 vs. NCBI nr
Match:
XP_022945852.1 (cytochrome P450 714C2-like [Cucurbita moschata])
HSP 1 Score: 886.3 bits (2289), Expect = 1.2e-253
Identity = 434/512 (84.77%), Postives = 468/512 (91.41%), Query Frame = 0
Query: 6 VSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKRTMKAAAAINGGSF 65
V +MVLFVKVV+SWW+LP+LAHQKLKRNGF GP PSFPLGNIAEMKR KA+AAING +
Sbjct: 14 VLVMVLFVKVVVSWWVLPVLAHQKLKRNGFRGPPPSFPLGNIAEMKRIRKASAAINGDAH 73
Query: 66 ASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGW 125
+ S+ VSHDIHS VFPHFAQWQASYGKKF+YWLGTEPFLYIADP F+KKISEAVM KGW
Sbjct: 74 SFPSSKVSHDIHSTVFPHFAQWQASYGKKFIYWLGTEPFLYIADPHFMKKISEAVMAKGW 133
Query: 126 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKW 185
GKPAVFRNDR PMFGDGLVMTEGDDW+RHRHILTPAFNPANLKAMTKFMVESTT+MLNKW
Sbjct: 134 GKPAVFRNDRRPMFGDGLVMTEGDDWVRHRHILTPAFNPANLKAMTKFMVESTTKMLNKW 193
Query: 186 SSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV 245
SSLVNSA EIDVEREITATAGDIIA+ASFGI N GGRDAF+ LRALQFTLFQNSTYVGV
Sbjct: 194 SSLVNSAGGEIDVEREITATAGDIIAEASFGICNDGGRDAFDYLRALQFTLFQNSTYVGV 253
Query: 246 PFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGG 305
PFS LF CP RTL+AKRLGA IDRLFLS++S+RK S++ SSSDLLSRLIE +++ GG
Sbjct: 254 PFSSLFFCPGRTLSAKRLGAAIDRLFLSLVSKRKLSQD--SSSDLLSRLIEGEKSTGDGG 313
Query: 306 KMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEIDF 365
KM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+ILREEIKEVIGDKEIDF
Sbjct: 314 KMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGDKEIDF 373
Query: 366 AMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTL 425
MLSGL+KMG VF+EVLRLYPSAPNIQRQAKGDI VG +TIPKGTNIWID+V+MH DP L
Sbjct: 374 NMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIDVGSMTIPKGTNIWIDIVAMHRDPDL 433
Query: 426 WGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFS 485
WG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG+NLSSMEYKIVLTLILSRFS
Sbjct: 434 WGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKNLSSMEYKIVLTLILSRFS 493
Query: 486 LSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 518
LSLSP+Y+HSPA LLSLRPAHGLPLIV PL P
Sbjct: 494 LSLSPNYAHSPATLLSLRPAHGLPLIVTPLHP 523
BLAST of Lcy11g001550 vs. NCBI nr
Match:
KAG6574181.1 (Cytochrome P450 714C2, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 885.9 bits (2288), Expect = 1.6e-253
Identity = 434/512 (84.77%), Postives = 468/512 (91.41%), Query Frame = 0
Query: 6 VSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKRTMKAAAAINGGSF 65
V +MVLFVKVV+SWW+LP+LAHQKLKRNGF GP PSFPLGNIAEMKR KA+AAING +
Sbjct: 14 VLVMVLFVKVVVSWWVLPVLAHQKLKRNGFRGPPPSFPLGNIAEMKRIRKASAAINGDAH 73
Query: 66 ASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGW 125
+ S+ VSHDIHS VFPHFAQWQASYGKKF+YWLGTEPFLYIADP F+KKISEAVM KGW
Sbjct: 74 SFPSSKVSHDIHSTVFPHFAQWQASYGKKFIYWLGTEPFLYIADPHFMKKISEAVMAKGW 133
Query: 126 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKW 185
GKPAVFRNDR PMFGDGLVMTEGDDW+RHRHILTPAFNPANLKAMTKFMVESTT+MLNKW
Sbjct: 134 GKPAVFRNDRRPMFGDGLVMTEGDDWVRHRHILTPAFNPANLKAMTKFMVESTTKMLNKW 193
Query: 186 SSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV 245
SSLVNSA EIDVEREITATAGDIIA+ASFGI N GGRDAF+ LRALQFTLFQNSTYVGV
Sbjct: 194 SSLVNSAGGEIDVEREITATAGDIIAKASFGICNDGGRDAFDYLRALQFTLFQNSTYVGV 253
Query: 246 PFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGG 305
PFS LF CP RTL+AKRLGA IDRLFLS++S+RK S++ SSSDLLSRLIE +++ GG
Sbjct: 254 PFSSLFFCPGRTLSAKRLGAAIDRLFLSLVSKRKLSQD--SSSDLLSRLIEGEKSTGDGG 313
Query: 306 KMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEIDF 365
KM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+ILREEIKEVIGDKEIDF
Sbjct: 314 KMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGDKEIDF 373
Query: 366 AMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTL 425
MLSGL+KMG VF+EVLRLYPSAPNIQRQAKGDI VG +TIPKGTNIWID+V+MH DP L
Sbjct: 374 NMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIDVGSMTIPKGTNIWIDIVAMHRDPDL 433
Query: 426 WGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFS 485
WG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG+NLSSMEYKIVLTLILSRFS
Sbjct: 434 WGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKNLSSMEYKIVLTLILSRFS 493
Query: 486 LSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 518
LSLSP+Y+HSPA LLSLRPAHGLPLIV PL P
Sbjct: 494 LSLSPNYAHSPATLLSLRPAHGLPLIVTPLHP 523
BLAST of Lcy11g001550 vs. NCBI nr
Match:
XP_022968014.1 (cytochrome P450 714C2-like [Cucurbita maxima])
HSP 1 Score: 880.9 bits (2275), Expect = 5.2e-252
Identity = 435/512 (84.96%), Postives = 467/512 (91.21%), Query Frame = 0
Query: 6 VSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKRTMKAAAAINGGSF 65
V IMVLFVKVV+SWW+LP+LAHQKLKRNGF GP P+FPLGNIAEMKR KA+AAING S
Sbjct: 14 VLIMVLFVKVVVSWWVLPVLAHQKLKRNGFRGPPPTFPLGNIAEMKRIRKASAAINGDSH 73
Query: 66 ASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGW 125
+ S+ VSHDIHS VFPHFAQWQASYGKKFVYWLGTEPFLYIADP F+K+IS AVM KGW
Sbjct: 74 SFPSSKVSHDIHSTVFPHFAQWQASYGKKFVYWLGTEPFLYIADPHFMKQISVAVMAKGW 133
Query: 126 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKW 185
GKPAVFRNDR PMFGDGLVMTEGD W+RHRHILTPAFNPANLKAMTKFMVESTT+MLN+W
Sbjct: 134 GKPAVFRNDRRPMFGDGLVMTEGDVWVRHRHILTPAFNPANLKAMTKFMVESTTKMLNEW 193
Query: 186 SSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV 245
SSLVNSA EIDVEREITATAGDIIA+ASFGI NGGGRDAF+ LRALQFTLFQNSTYVGV
Sbjct: 194 SSLVNSASGEIDVEREITATAGDIIAKASFGICNGGGRDAFDYLRALQFTLFQNSTYVGV 253
Query: 246 PFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGG 305
PFS LF CP RTLAAKRLGA IDRLFLS++SERK S+ +SSSDLLSRLIE Q++ G
Sbjct: 254 PFSSLFFCPGRTLAAKRLGAAIDRLFLSLVSERKLSQ--NSSSDLLSRLIEGQKSTGDVG 313
Query: 306 KMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEIDF 365
KM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+ILREEIKEVIGDKEIDF
Sbjct: 314 KMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGDKEIDF 373
Query: 366 AMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTL 425
MLSGL+KMG VF+EVLRLYPSAPNIQRQAKGDI+VG +TIPKGTNIWID+V+MH DP L
Sbjct: 374 NMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIEVGSMTIPKGTNIWIDIVAMHRDPDL 433
Query: 426 WGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFS 485
WG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG++LSSMEYKIVLTLILSRFS
Sbjct: 434 WGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKSLSSMEYKIVLTLILSRFS 493
Query: 486 LSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 518
LSLSP+YSHSPA LLSLRPAHGLPLIV PL P
Sbjct: 494 LSLSPNYSHSPATLLSLRPAHGLPLIVTPLHP 523
BLAST of Lcy11g001550 vs. NCBI nr
Match:
XP_023542056.1 (cytochrome P450 714C2-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 878.2 bits (2268), Expect = 3.4e-251
Identity = 432/512 (84.38%), Postives = 465/512 (90.82%), Query Frame = 0
Query: 6 VSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKRTMKAAAAINGGSF 65
V +MVLF+KVV+SWW+LP+LA QKLKRNGF GP PSFPLGNIAEMKR KA+AAING
Sbjct: 14 VLVMVLFLKVVVSWWVLPVLALQKLKRNGFRGPPPSFPLGNIAEMKRIRKASAAINGDPH 73
Query: 66 ASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGW 125
+ S+ VSHDIHS VFPHFAQWQASYGKKF+YWLGTEPFLYIADP F+KKISEAVM KGW
Sbjct: 74 SFPSSKVSHDIHSTVFPHFAQWQASYGKKFIYWLGTEPFLYIADPHFMKKISEAVMAKGW 133
Query: 126 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKW 185
GKPAVFRNDR PMFGDGLVMTEGDDW+RHRHILTPAFNPANLKAMTKFMVESTT+MLNKW
Sbjct: 134 GKPAVFRNDRRPMFGDGLVMTEGDDWVRHRHILTPAFNPANLKAMTKFMVESTTKMLNKW 193
Query: 186 SSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV 245
SSLVNSA EIDVER ITATAGDIIA+ASFGI N GGRDAF+ LRALQFTLFQNSTYVGV
Sbjct: 194 SSLVNSAGGEIDVERGITATAGDIIAKASFGICNDGGRDAFDYLRALQFTLFQNSTYVGV 253
Query: 246 PFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIESQRNGSGGG 305
PFS LF CP RTL+AKRLGA IDRLFLS++S+RK S++ SSSDLLSRLIE +++ GG
Sbjct: 254 PFSSLFFCPGRTLSAKRLGAAIDRLFLSLVSKRKLSQD--SSSDLLSRLIEGEKSTGDGG 313
Query: 306 KMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEIDF 365
KM LTTRELVDECKTFFFGGHETTALA SWTLLLLATRPEWQ+ILREEIKEVIGDKEIDF
Sbjct: 314 KMGLTTRELVDECKTFFFGGHETTALATSWTLLLLATRPEWQDILREEIKEVIGDKEIDF 373
Query: 366 AMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDPTL 425
MLSGL+KMG VF+EVLRLYPSAPNIQRQAKGDI VG +TIPKGTNIWID+V+MH DP L
Sbjct: 374 NMLSGLKKMGCVFSEVLRLYPSAPNIQRQAKGDIDVGSMTIPKGTNIWIDIVAMHRDPDL 433
Query: 426 WGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSRFS 485
WG+D NEFKPERF +DSI GGCNHKMGYLPFGFGGRMCIG+NLSSMEYKIVLTLILSRFS
Sbjct: 434 WGDDANEFKPERFVEDSIRGGCNHKMGYLPFGFGGRMCIGKNLSSMEYKIVLTLILSRFS 493
Query: 486 LSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 518
LSLSP+YSHSPA LLSLRPAHGLPLIV PL P
Sbjct: 494 LSLSPNYSHSPATLLSLRPAHGLPLIVTPLHP 523
BLAST of Lcy11g001550 vs. NCBI nr
Match:
XP_038877847.1 (cytokinin hydroxylase-like [Benincasa hispida])
HSP 1 Score: 877.1 bits (2265), Expect = 7.5e-251
Identity = 433/519 (83.43%), Postives = 468/519 (90.17%), Query Frame = 0
Query: 1 MWAIRVSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKRTMKAAAAI 60
MWAIR +IMV F KVV+SWWILPILAH+ LKRNGFEGPSP+FPLGNI EMK+ +KAAAAI
Sbjct: 12 MWAIRGTIMVFFGKVVVSWWILPILAHKNLKRNGFEGPSPTFPLGNITEMKKIIKAAAAI 71
Query: 61 NGGSFASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAV 120
NGGS +SSNN+SHDIHS +FPHFAQWQ SYGKKFVYWLGTEPFLYIADPQ VKKISEAV
Sbjct: 72 NGGSSLASSNNLSHDIHSTIFPHFAQWQNSYGKKFVYWLGTEPFLYIADPQLVKKISEAV 131
Query: 121 MGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQ 180
MGK WGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNP NLKAMTKFM+ESTT+
Sbjct: 132 MGKSWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPTNLKAMTKFMLESTTK 191
Query: 181 MLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISN-GGGRDAFNSLRALQFTLFQN 240
M+ +W SLVNS Q EIDVEREITATAGDIIA+ASFGIS+ GGG +A NSLRALQ TLF N
Sbjct: 192 MVKEWRSLVNSGQSEIDVEREITATAGDIIAKASFGISSGGGGEEALNSLRALQLTLFSN 251
Query: 241 STYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQ-SEENSSSSDLLSRLIESQ 300
S+YVGVPFS LFL P RTLAAKRLGA+IDRLF S+IS RK S + S SDLLSRLI+SQ
Sbjct: 252 SSYVGVPFSSLFLYPGRTLAAKRLGAQIDRLFFSIISHRKLISSGSDSDSDLLSRLIQSQ 311
Query: 301 RNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVI 360
N GG LT RE+VDECKTFFFGGHETTALAISWTLLLLATRPEWQ ILREEIKEVI
Sbjct: 312 SNKYKGG-AALTPREVVDECKTFFFGGHETTALAISWTLLLLATRPEWQKILREEIKEVI 371
Query: 361 GDKEIDFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVS 420
GDK+IDF+MLS L+KMGWV+NEVLRLYPSAPNIQRQAKGDI +GD+ IPKGTNIWID+V+
Sbjct: 372 GDKDIDFSMLSALKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDMKIPKGTNIWIDIVA 431
Query: 421 MHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLT 480
MHHDPTLWG+DVNEFKPERF+ D+IHGGCNHKMGYLPFGFGGRMCIGRNLSS EYKIVLT
Sbjct: 432 MHHDPTLWGDDVNEFKPERFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSTEYKIVLT 491
Query: 481 LILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPLQP 518
LILS FSLSLSPSYSHSPAILLSLRP HG+PLI+ PL P
Sbjct: 492 LILSTFSLSLSPSYSHSPAILLSLRPTHGIPLILTPLHP 529
BLAST of Lcy11g001550 vs. TAIR 10
Match:
AT5G52400.1 (cytochrome P450, family 715, subfamily A, polypeptide 1 )
HSP 1 Score: 631.7 bits (1628), Expect = 5.1e-181
Identity = 302/512 (58.98%), Postives = 385/512 (75.20%), Query Frame = 0
Query: 6 VSIMVLFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKRTMKAAAAINGGSF 65
V I ++F+K+ + WI P+ A +KL+ NGF GP+PSFP GN+ +MK+ A+ ++
Sbjct: 19 VVICLMFLKLFLRCWIWPVRAQKKLRGNGFVGPAPSFPFGNLNDMKKLKMASVVVDN--- 78
Query: 66 ASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGW 125
+ SS ++HDIHSI PHFA+WQ YGK FVYWLG EPF+Y+ADP+F+ +S+ V+GK W
Sbjct: 79 SKSSTIINHDIHSIALPHFARWQQEYGKVFVYWLGIEPFVYVADPEFLSVMSKGVLGKSW 138
Query: 126 GKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKW 185
GKP VF+ DREPMFG GLVM EGDDW RHRHI+TPAF P NLK MT MVES + ML++W
Sbjct: 139 GKPNVFKKDREPMFGTGLVMVEGDDWTRHRHIITPAFAPLNLKVMTNMMVESVSNMLDRW 198
Query: 186 SSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGV 245
+NS PE D+E EI TAG+IIA+ SFG++ G +LRA+QF LF ++ YVGV
Sbjct: 199 GIQINSGNPEFDMESEIIGTAGEIIAKTSFGVTGENGTQVLKNLRAVQFALFNSNRYVGV 258
Query: 246 PFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQS--EENSSSSDLLSRLIESQRNGSG 305
PFS + L +T+ AK LG EID L LS I++RK S E + DLL L+++ + G+
Sbjct: 259 PFSNI-LSYKQTVKAKGLGHEIDGLLLSFINKRKISLAEGDDQGHDLLGMLLKADQKGN- 318
Query: 306 GGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEVIGDKEI 365
T +ELVDECKTFFF GHETTALA++WT +LLA PEWQ+ +REEI+EVIGD +I
Sbjct: 319 -----FTAKELVDECKTFFFAGHETTALALTWTFMLLAIHPEWQDTIREEIREVIGDSKI 378
Query: 366 DFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVVSMHHDP 425
++ L+GL+KM WV NEVLRLYP APN QRQA+ DI+V IP GTNIWIDVV+MHHD
Sbjct: 379 EYNKLAGLKKMSWVMNEVLRLYPPAPNAQRQARNDIEVNGRVIPNGTNIWIDVVAMHHDV 438
Query: 426 TLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSR 485
LWG+DVNEFKPERF+ ++HGGC +KMGY+PFGFGGRMCIGRNL++MEYKIVL+L+LSR
Sbjct: 439 ELWGDDVNEFKPERFD-GNLHGGCKNKMGYMPFGFGGRMCIGRNLTTMEYKIVLSLVLSR 498
Query: 486 FSLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 516
F +S+SP Y HSP +LSLRP +GLPLI++PL
Sbjct: 499 FEISVSPGYRHSPTYMLSLRPGYGLPLIIRPL 519
BLAST of Lcy11g001550 vs. TAIR 10
Match:
AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )
HSP 1 Score: 327.4 bits (838), Expect = 2.1e-89
Identity = 184/520 (35.38%), Postives = 284/520 (54.62%), Query Frame = 0
Query: 4 IRVSIMVLFVKV----VISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKRTMKAAAA 63
+ V +M L ++V + +++ P + ++R G GP P GNI ++ + + +A
Sbjct: 10 VLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDISKMLSHSA- 69
Query: 64 INGGSFASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKI--- 123
++ +++ H+I + PH+ W YGK+F+ W GTEP L + + + +K++
Sbjct: 70 ------SNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELLTK 129
Query: 124 SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVE 183
V GK W + + + G GL+M G+ W RH+ PAF LK K MVE
Sbjct: 130 HNPVTGKSW----LQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVE 189
Query: 184 STTQMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTL 243
T M + L E+++ E+ DII++ FG S G++ F+ L LQ
Sbjct: 190 CTKMMAER---LRKEVGEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLC 249
Query: 244 FQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSSDLLSRLIE 303
Q + ++ P S FL K L E++RL + +I RK S E SS L+
Sbjct: 250 AQATRHLCFPGS-RFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLG 309
Query: 304 SQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKE 363
N K L + ++DECKTFFF GHETT+L ++WTL+LLA P WQ+ +R+E+++
Sbjct: 310 LLLNQMDSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVRQ 369
Query: 364 VIGDKEI-DFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWID 423
V G + LS L + V NE LRLYP A + R A DIK+GD+ IPKG +IWI
Sbjct: 370 VCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWIP 429
Query: 424 VVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKI 483
V+++HH LWGED NEF PERF S H ++PF G R CIG+ + ME KI
Sbjct: 430 VLAIHHSNELWGEDANEFNPERFTTRSF-ASSRH---FMPFAAGPRNCIGQTFAMMEAKI 489
Query: 484 VLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 516
+L +++S+FS ++S +Y H+P ++L+++P +G+ L++KPL
Sbjct: 490 ILAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPL 510
BLAST of Lcy11g001550 vs. TAIR 10
Match:
AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )
HSP 1 Score: 326.2 bits (835), Expect = 4.6e-89
Identity = 184/527 (34.91%), Postives = 284/527 (53.89%), Query Frame = 0
Query: 7 SIMVLFVKVVI--------SWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKRTMKAAA 66
S++V+FV ++ +W+ P + +++ G GP P GNI E+ +
Sbjct: 8 SLLVIFVTTILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEI-------S 67
Query: 67 AINGGSFASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKI-- 126
A+ S + +++ HDI + PH+ W YGK+F+ W GT+P L + + + +K++
Sbjct: 68 AMVSQSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLM 127
Query: 127 -SEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMV 186
V G+ W + +N G GL+M G DW RH+ PAF LK + MV
Sbjct: 128 KHNGVSGRSWLQQQGTKN----FIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMV 187
Query: 187 ESTTQMLNKWSSLVNSAQPEIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFT 246
E T++++ + V E+++ E+ DII++ FG S G++ FN L LQ
Sbjct: 188 ECTSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRR 247
Query: 247 LFQNSTYVGVPFSGLFLCPARTLAAKRLGAEIDRLFLSVISERKQSEENSSSS----DLL 306
Q + ++ P S FL K L E++RL + +I R+ E SS DLL
Sbjct: 248 CAQATRHLCFPGS-RFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLL 307
Query: 307 SRLIESQRNGSGGGKMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILR 366
L+ + ++DECKTFFF GHETTAL ++WT +LLA P WQ +R
Sbjct: 308 GLLLNEMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVR 367
Query: 367 EEIKEVIGDKEI-DFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGT 426
EE++EV G + LS L + V NE LRLYP A + R A D+K+GD+TIPKG
Sbjct: 368 EEVREVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGL 427
Query: 427 NIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMG--YLPFGFGGRMCIGRNL 486
+IWI V+++HH LWG+D N+F PERF GG G ++PF G R CIG+
Sbjct: 428 SIWIPVLAIHHSEELWGKDANQFNPERF------GGRPFASGRHFIPFAAGPRNCIGQQF 487
Query: 487 SSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIVKPL 516
+ ME KI+L ++S+F+ ++S +Y H+P ++L+++P +G+ +I+KPL
Sbjct: 488 ALMEAKIILATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516
BLAST of Lcy11g001550 vs. TAIR 10
Match:
AT5G24900.1 (cytochrome P450, family 714, subfamily A, polypeptide 2 )
HSP 1 Score: 307.0 bits (785), Expect = 2.9e-83
Identity = 173/515 (33.59%), Postives = 273/515 (53.01%), Query Frame = 0
Query: 11 LFVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKRTMKAAAAINGGSFASSSN 70
++ + V+ W + + LK G +GP PS GN++EM+R A S N
Sbjct: 27 VYGRAVVEQWRM----RRSLKLQGVKGPPPSIFNGNVSEMQRIQSEA------KHCSGDN 86
Query: 71 NVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKISEAVMGKGWGKPAV 130
+SHD S +FPHF W+ YG+ + Y G + LYI P+ VK++S+ G+
Sbjct: 87 IISHDYSSSLFPHFDHWRKQYGRIYTYSTGLKQHLYINHPEMVKELSQ-TNTLNLGRITH 146
Query: 131 FRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVESTTQMLNKWSSLVN 190
P+ G+G++ + G W R I+ F +K M MVES MLNKW +V
Sbjct: 147 ITKRLNPILGNGIITSNGPHWAHQRRIIAYEFTHDKIKGMVGLMVESAMPMLNKWEEMVK 206
Query: 191 SAQP---EIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQFTLFQNSTYVGVPF 250
+I V+ ++ + D+IA+A FG S G+ F+ +R L + + S V F
Sbjct: 207 RGGEMGCDIRVDEDLKDVSADVIAKACFGSSFSKGKAIFSMIRDLLTAITKRS--VLFRF 266
Query: 251 SGLFLCPARTLAAKRLG-AEIDRLFLSV-------ISERKQSEENSSSSDLLSRLIESQR 310
+G +K+ G +ID L + + + ER+ +++ DL+ ++E
Sbjct: 267 NGF---TDMVFGSKKHGDVDIDALEMELESSIWETVKEREIECKDTHKKDLMQLILEGAM 326
Query: 311 NGSGGGKMRLTT--RELVDECKTFFFGGHETTALAISWTLLLLATRPEWQNILREEIKEV 370
G + R +VD CK+ +F GH++TA+++SW L+LLA P WQ +R+EI
Sbjct: 327 RSCDGNLWDKSAYRRFVVDNCKSIYFAGHDSTAVSVSWCLMLLALNPSWQVKIRDEILSS 386
Query: 371 IGDKEIDFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPKGTNIWIDVV 430
+ D + L+ + V E +RLYP AP + R+A DI++GD+ +PKG IW +
Sbjct: 387 CKNGIPDAESIPNLKTVTMVIQETMRLYPPAPIVGREASKDIRLGDLVVPKGVCIWTLIP 446
Query: 431 SMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVL 490
++H DP +WG D N+FKPERF + I C + Y+PFG G R C+G+N ME K+++
Sbjct: 447 ALHRDPEIWGPDANDFKPERF-SEGISKACKYPQSYIPFGLGPRTCVGKNFGMMEVKVLV 506
Query: 491 TLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIV 513
+LI+S+FS +LSP+Y HSP+ L + P HG+ + V
Sbjct: 507 SLIVSKFSFTLSPTYQHSPSHKLLVEPQHGVVIRV 524
BLAST of Lcy11g001550 vs. TAIR 10
Match:
AT5G24910.1 (cytochrome P450, family 714, subfamily A, polypeptide 1 )
HSP 1 Score: 302.0 bits (772), Expect = 9.3e-82
Identity = 168/524 (32.06%), Postives = 277/524 (52.86%), Query Frame = 0
Query: 2 WAIRVSIMVL----FVKVVISWWILPILAHQKLKRNGFEGPSPSFPLGNIAEMKRTMKAA 61
W + + ++ L + KV+ W + +KL G +GP PS GN+ EM++ +
Sbjct: 14 WIVVIGVLGLGIRVYGKVMAEQWRM----RRKLTMQGVKGPPPSLFRGNVPEMQKIQ--S 73
Query: 62 AAINGGSFASSSNNVSHDIHSIVFPHFAQWQASYGKKFVYWLGTEPFLYIADPQFVKKIS 121
++ S N ++HD S +FP+ W+ YG+ + Y G + LY+ P+ VK+++
Sbjct: 74 QIMSNSKHYSGDNIIAHDYTSSLFPYLDHWRKQYGRVYTYSTGVKQHLYMNHPELVKELN 133
Query: 122 EAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDWIRHRHILTPAFNPANLKAMTKFMVES 181
+A GK + + + G G++ + G W R I+ P F +K M +VES
Sbjct: 134 QA-NTLNLGKVSYVTKRLKSILGRGVITSNGPHWAHQRRIIAPEFFLDKVKGMVGLVVES 193
Query: 182 TTQMLNKWSSLVNSAQP---EIDVEREITATAGDIIAQASFGISNGGGRDAFNSLRALQF 241
ML+KW ++ +I V+ ++ A + D+I++A FG S G++ F+ LR LQ
Sbjct: 194 AMPMLSKWEEMMKREGEMVCDIIVDEDLRAASADVISRACFGSSFSKGKEIFSKLRCLQK 253
Query: 242 TLFQNSTYVGV-PFSGLFLCPARTLAAK--RLGAEIDRLFLSVISERKQSEENSSSSDLL 301
+ N+ + F+ + + K L I+ L + ER++ DL+
Sbjct: 254 AITHNNILFSLNGFTDVVFGTKKHGNGKIDELERHIESLIWETVKERERECVGDHKKDLM 313
Query: 302 SRLIESQRNGSGGG---KMRLTTRELVDECKTFFFGGHETTALAISWTLLLLATRPEWQN 361
++E R+ G K + +VD CK+ +F GHET+A+A+SW L+LLA P WQ
Sbjct: 314 QLILEGARSSCDGNLEDKTQSYKSFVVDNCKSIYFAGHETSAVAVSWCLMLLALNPSWQT 373
Query: 362 ILREEIKEVIGDKEIDFAMLSGLRKMGWVFNEVLRLYPSAPNIQRQAKGDIKVGDVTIPK 421
+R+E+ + D +S L+ + V E LRLYP A + R+A D K+G++ +PK
Sbjct: 374 RIRDEVFLHCKNGIPDADSISNLKTVTMVIQETLRLYPPAAFVSREALEDTKLGNLVVPK 433
Query: 422 GTNIWIDVVSMHHDPTLWGEDVNEFKPERFEKDSIHGGCNHKMGYLPFGFGGRMCIGRNL 481
G IW + ++H DP +WG D NEF PERF + + C H ++PFG G R+C+G+N
Sbjct: 434 GVCIWTLIPTLHRDPEIWGADANEFNPERF-SEGVSKACKHPQSFVPFGLGTRLCLGKNF 493
Query: 482 SSMEYKIVLTLILSRFSLSLSPSYSHSPAILLSLRPAHGLPLIV 513
ME K++++LI+SRFS +LSP+Y HSP + + P HG+ + V
Sbjct: 494 GMMELKVLVSLIVSRFSFTLSPTYQHSPVFRMLVEPQHGVVIRV 529
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q2QYH7 | 7.7e-89 | 35.81 | Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 ... | [more] |
| Q9ZW95 | 2.9e-88 | 35.38 | Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1 | [more] |
| Q9FF18 | 6.5e-88 | 34.91 | Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1 | [more] |
| B6SSW8 | 4.8e-83 | 34.92 | Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1 | [more] |
| Q6NKZ8 | 4.1e-82 | 33.59 | Cytochrome P450 714A2 OS=Arabidopsis thaliana OX=3702 GN=CYP714A2 PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1G224 | 6.0e-254 | 84.77 | cytochrome P450 714C2-like OS=Cucurbita moschata OX=3662 GN=LOC111449972 PE=3 SV... | [more] |
| A0A6J1HYD5 | 2.5e-252 | 84.96 | cytochrome P450 714C2-like OS=Cucurbita maxima OX=3661 GN=LOC111467387 PE=3 SV=1 | [more] |
| A0A6J1CIQ8 | 1.1e-250 | 83.21 | cytokinin hydroxylase OS=Momordica charantia OX=3673 GN=LOC111011538 PE=3 SV=1 | [more] |
| A0A1S3AXX2 | 2.5e-244 | 81.82 | cytokinin hydroxylase OS=Cucumis melo OX=3656 GN=LOC103484084 PE=3 SV=1 | [more] |
| A0A5D3DI76 | 1.9e-220 | 81.24 | Cytokinin hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold38... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT5G52400.1 | 5.1e-181 | 58.98 | cytochrome P450, family 715, subfamily A, polypeptide 1 | [more] |
| AT1G67110.1 | 2.1e-89 | 35.38 | cytochrome P450, family 735, subfamily A, polypeptide 2 | [more] |
| AT5G38450.1 | 4.6e-89 | 34.91 | cytochrome P450, family 735, subfamily A, polypeptide 1 | [more] |
| AT5G24900.1 | 2.9e-83 | 33.59 | cytochrome P450, family 714, subfamily A, polypeptide 2 | [more] |
| AT5G24910.1 | 9.3e-82 | 32.06 | cytochrome P450, family 714, subfamily A, polypeptide 1 | [more] |
