Lcy09g021070 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g021070
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionprotein AUXIN SIGNALING F-BOX 2-like
LocationChr09: 46582585 .. 46585926 (-)
RNA-Seq ExpressionLcy09g021070
SyntenyLcy09g021070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTATTTTTTCTCTCTCACTAGACAAGAGCCAGTGTGGGGGCCTCTCTCTCTTCGAAACAAGAGATAAGGACACATGCATGTTCACCCATGGTTAATTTTCCTCTGGTTAAAAACTAACCATGGTTACCTTTCTCCCCGCTCAACCTCTTCCTCCTCTCTGTTTCTTCTCTGACTCTCTCTGTTTCACCTCCAGTTCGTTTTTCAGTGGGAGAGACGACGCTGTTCTTCTTCTCTATTTTTCATTTTTTTTTTCAATCTCTCTCTCTCTCTTGTTCATCTTCATCATTAGAATCCAATCCTGTGTTTAATTTCTGTACATATTTCTTTCTGTTTCTTCTTCCACGGATCTAATCGTTTCCATTTCGAACTGGGTCATCGTCTTCATCGGACTTCAATCGCCTGAATTTCCTTCCACAACTGCGGCAATTTTTGTTCCACTTTCTGTTTCTTCTTCGGAATATTCCACTTCCTTTGCCCCAATCTCTCTCTCTCTCTCTCTCTCTCTCTGCAATTTCAGATCTAGGTTTTTGAACTGGTCGGGATGCAAGATTTGTTCTTATATTCTCTTCAGCTCCTTGGATCTGTCGACTTCGCTGCGGATTTCGAATTCCACTAGACAATCCATTGGAGATCTGCTTCAATTCTTCCTGGAGGATTTTTCTTCTGGTTTGTTTGTTTGTTTTTTTTTTGAGTTTCTTTCTTTATTCAGGGCTTCACGAGCTTTTTTCCGGTATGTAATAGTGCTGGATAAAGAGAGTTTTTCTCGTTTTTTTTTTCTTTTTTGGAGGTTAAATATAATTTTCCTCGGAAAGTTTATTGTTCTTCTTCTTCTTCTTTTTCCTTTCCCTGGGGAAAGTTGCGTCCATTTTTAATTTTAATTTTTTCTTTCCTGGAAAGTTTTTGGGGAATTTTCCGTTTGGATGCTGAGAAACGATGAATTATTTTCCCGACGAGGTTTTAGAACATGTTTTCGACTATGTTACGTCTCACCGGGACCGGAACGCGGTGTCGCTGGTGTGCAAATTGTGGTACAGAGTTGAGAGATTTAGCAGAACGAAAGTGTTCGTCGGAAATTGCTATGCGATCACGCCGGAGAGGCTAATCGGAAGATTCCCCGGCGTCAAATCCCTAACCCTAAAGGGAAAGCCTCATTTCGCCGATTTCAATTTGGTCCCTCATGATTGGGGCGGCTATGTCTACCCTTGGATCCAAGCCTTTGCCAAGCGTCGGATTGCCCTCGAGGAGCTCCGTTTGAAGCGGATGGTCGTCACTGACGACAGTCTTGAGCTCCTTTCCCGATCCTTCTCCAATTTCAAGTCGCTGTTCCTCTTCAGCTGTGAGGGATTCACCACCAATGGCCTTGCTGCCATTGCTGCCAATTGCAGGTATAAAACTCCCTTTCTTTTGAGCTTTGGTTTGTTGGGTTGTAATTAGAAACATTACTACTCTTGCTTCTCGTTGCTCTATGTATGTTTTTGACATTCTTGTTCTGTCTGAGCTGAGCATTTCAAGGATGGAGGGTCTGTTGTGTTGATTGTTGTTGACTTTAGAGCTATCCTTTTGAGAGCTTTTGCTGTCAGTTGGTTGATCTTTAGCTGCTGTGTAGATTGATTTTTTGTAGATGAAATTCGTTGGGTATTTTGGTGATGAGATTACGGTTTTGTGATGTGTTTTGTTTTCCATTTGTTGTCGTGTTTTCGTTCGGATTAGGTTTCTGAGGGAGCTGGACCTGCAAGAGAATGAAATTGATGACCATAGTAATTACTGGCTTAGCTGCTTTCCAGAGAACTGCACATCGCTTGTCTCCCTGAATTTTGCTTGCCTCAGAGGAGAAGTAAATCTGGGTGCTCTTGAGAGGCTTGTGGCGAGATCTCCAAACCTCAAGAGTTTGAGGCTGAACCGTGCGGTGCCTCTTGAAACCTTGCATAGTATATTGGAACGCGCTCCGCAACTTGTGGACTTGGGCACCGGGTCTTACGTTCACGATCGAGATTCCGAAATCTACGACAATCTTAAGAACACCATTCTGAAATGCAAATCGATCAGGAGTTTATCTGGATTTTTAGATGTTTCTCCTCGCTGCCTGGCCTCCATTTACCCCATTTGCTCAAATTTGACATCTTTGAACCTGAGCTATGCTCCTGGGCTTCATGGCAGTGAGCTCATAAAGGTTATTCGGTATTGCGAGAAGCTTCAGCGCTTATGGGTATGATCTTATTACGAACTTAATACAGTATAAACCATTATATGTATATGATTTCTCCTGTATGTGTGGGTCTATTTTATACGTTTTTGAATGATGAGAAATGTTATTGGCAATGTCCGCAGATTCTGGATGGTATTGGAGACAAAGGACTCGAAGTTGTTGCTTCAACTTGTAAAGAACTGCAGGAATTGAGGGTTTTCCCATCTGATCTCTCTGGGGCTGGTAATGTTGCTGTCACAGAAGAAGGTTTGGTTGCTATATCGATGGGTTGCCCGAAACTTCATTCGATACTATACTTCTGCCATCAGATGACAAATGCTGCCCTTGTAACGGTAGCAAAGAACAACCCGAACTTCATACGCTTCAGGTTGTGCATCCTCAACCCCACGAAACCGGACCCTGTAACTGGGAATGCACTGGATGAAGGTTTTGGGGCGATTGTCCAAGCGTGCAAAGGTCTGAGACGTTTATCGCTCTCAGGCCTTCTTACAGATCAGGTCTTCTACTACATCGGTGAATACGCAAAGCATCTCGAAATGCTTTCTTTAGCATTTGCTGGGGACAGTGACAAGGGAATGATTCACGTATTGAACGGTTGCAAGAAACTTCGCAAGCTCGAGATCATGGACAGCCCGTTCGGCGACATGGCACTTCTGCAGGACGTCGGGAAGTATGAAACAATGCGATCCCTTTGGATGTCGTCCTGCCAGATTACTCTTGGTGGCTGCAAGACGCTAGCGAAGAAGATGCCGAGGCTGAACGTGGAGATCATCGACGAGGACGATCAGTCGGAGTTCTGCCGTGATGATGGGCAGAAAGTAGGGAAAATGTATCTGTACCGTACATTAGTAGGGCCGAGGAAAGATGCACCCAAGTTTGTGTGGACATTGTAGATTTGATGATACTTCTTTCCTTGAAGGCTTTTGAATTACTTGTACTTTCTTTTTTCTTCTTTAGGGCTACTGACCAAGTGGGATTTTTATTTAGCTACGTGAGTTTAGTGTATTGGTGTTATTGTAATTATTGGGAGTTTTTCAATTTGTAATCTGCATTGATGTTATAGACAGACTTTGCAATTCCAGTTTTCTCATTTCTCAAATGCTTGTGGATCTCGCATCAGCCACAATCTGTTTATA

mRNA sequence

TTATTTTTTCTCTCTCACTAGACAAGAGCCAGTGTGGGGGCCTCTCTCTCTTCGAAACAAGAGATAAGGACACATGCATGTTCACCCATGGTTAATTTTCCTCTGGTTAAAAACTAACCATGGTTACCTTTCTCCCCGCTCAACCTCTTCCTCCTCTCTGTTTCTTCTCTGACTCTCTCTGTTTCACCTCCAGTTCGTTTTTCAGTGGGAGAGACGACGCTGTTCTTCTTCTCTATTTTTCATTTTTTTTTTCAATCTCTCTCTCTCTCTTGTTCATCTTCATCATTAGAATCCAATCCTGTGTTTAATTTCTGTACATATTTCTTTCTGTTTCTTCTTCCACGGATCTAATCGTTTCCATTTCGAACTGGGTCATCGTCTTCATCGGACTTCAATCGCCTGAATTTCCTTCCACAACTGCGGCAATTTTTGTTCCACTTTCTGTTTCTTCTTCGGAATATTCCACTTCCTTTGCCCCAATCTCTCTCTCTCTCTCTCTCTCTCTCTGCAATTTCAGATCTAGGTTTTTGAACTGGTCGGGATGCAAGATTTGTTCTTATATTCTCTTCAGCTCCTTGGATCTGTCGACTTCGCTGCGGATTTCGAATTCCACTAGACAATCCATTGGAGATCTGCTTCAATTCTTCCTGGAGGATTTTTCTTCTGGTTTGTTTGTTTGTTTTTTTTTTGAGTTTCTTTCTTTATTCAGGGCTTCACGAGCTTTTTTCCGGTATGTAATAGTGCTGGATAAAGAGAGTTTTTCTCGTTTTTTTTTTCTTTTTTGGAGGTTAAATATAATTTTCCTCGGAAAGTTTATTGTTCTTCTTCTTCTTCTTTTTCCTTTCCCTGGGGAAAGTTGCGTCCATTTTTAATTTTAATTTTTTCTTTCCTGGAAAGTTTTTGGGGAATTTTCCGTTTGGATGCTGAGAAACGATGAATTATTTTCCCGACGAGGTTTTAGAACATGTTTTCGACTATGTTACGTCTCACCGGGACCGGAACGCGGTGTCGCTGGTGTGCAAATTGTGGTACAGAGTTGAGAGATTTAGCAGAACGAAAGTGTTCGTCGGAAATTGCTATGCGATCACGCCGGAGAGGCTAATCGGAAGATTCCCCGGCGTCAAATCCCTAACCCTAAAGGGAAAGCCTCATTTCGCCGATTTCAATTTGGTCCCTCATGATTGGGGCGGCTATGTCTACCCTTGGATCCAAGCCTTTGCCAAGCGTCGGATTGCCCTCGAGGAGCTCCGTTTGAAGCGGATGGTCGTCACTGACGACAGTCTTGAGCTCCTTTCCCGATCCTTCTCCAATTTCAAGTCGCTGTTCCTCTTCAGCTGTGAGGGATTCACCACCAATGGCCTTGCTGCCATTGCTGCCAATTGCAGGTTTCTGAGGGAGCTGGACCTGCAAGAGAATGAAATTGATGACCATAGTAATTACTGGCTTAGCTGCTTTCCAGAGAACTGCACATCGCTTGTCTCCCTGAATTTTGCTTGCCTCAGAGGAGAAGTAAATCTGGGTGCTCTTGAGAGGCTTGTGGCGAGATCTCCAAACCTCAAGAGTTTGAGGCTGAACCGTGCGGTGCCTCTTGAAACCTTGCATAGTATATTGGAACGCGCTCCGCAACTTGTGGACTTGGGCACCGGGTCTTACGTTCACGATCGAGATTCCGAAATCTACGACAATCTTAAGAACACCATTCTGAAATGCAAATCGATCAGGAGTTTATCTGGATTTTTAGATGTTTCTCCTCGCTGCCTGGCCTCCATTTACCCCATTTGCTCAAATTTGACATCTTTGAACCTGAGCTATGCTCCTGGGCTTCATGGCAGTGAGCTCATAAAGGTTATTCGGTATTGCGAGAAGCTTCAGCGCTTATGGATTCTGGATGGTATTGGAGACAAAGGACTCGAAGTTGTTGCTTCAACTTGTAAAGAACTGCAGGAATTGAGGGTTTTCCCATCTGATCTCTCTGGGGCTGGTAATGTTGCTGTCACAGAAGAAGGTTTGGTTGCTATATCGATGGGTTGCCCGAAACTTCATTCGATACTATACTTCTGCCATCAGATGACAAATGCTGCCCTTGTAACGGTAGCAAAGAACAACCCGAACTTCATACGCTTCAGGTTGTGCATCCTCAACCCCACGAAACCGGACCCTGTAACTGGGAATGCACTGGATGAAGGTTTTGGGGCGATTGTCCAAGCGTGCAAAGGTCTGAGACGTTTATCGCTCTCAGGCCTTCTTACAGATCAGGTCTTCTACTACATCGGTGAATACGCAAAGCATCTCGAAATGCTTTCTTTAGCATTTGCTGGGGACAGTGACAAGGGAATGATTCACGTATTGAACGGTTGCAAGAAACTTCGCAAGCTCGAGATCATGGACAGCCCGTTCGGCGACATGGCACTTCTGCAGGACGTCGGGAAGTATGAAACAATGCGATCCCTTTGGATGTCGTCCTGCCAGATTACTCTTGGTGGCTGCAAGACGCTAGCGAAGAAGATGCCGAGGCTGAACGTGGAGATCATCGACGAGGACGATCAGTCGGAGTTCTGCCGTGATGATGGGCAGAAAGTAGGGAAAATGTATCTGTACCGTACATTAGTAGGGCCGAGGAAAGATGCACCCAAGTTTGTGTGGACATTGTAGATTTGATGATACTTCTTTCCTTGAAGGCTTTTGAATTACTTGTACTTTCTTTTTTCTTCTTTAGGGCTACTGACCAAGTGGGATTTTTATTTAGCTACGTGAGTTTAGTGTATTGGTGTTATTGTAATTATTGGGAGTTTTTCAATTTGTAATCTGCATTGATGTTATAGACAGACTTTGCAATTCCAGTTTTCTCATTTCTCAAATGCTTGTGGATCTCGCATCAGCCACAATCTGTTTATA

Coding sequence (CDS)

ATGAATTATTTTCCCGACGAGGTTTTAGAACATGTTTTCGACTATGTTACGTCTCACCGGGACCGGAACGCGGTGTCGCTGGTGTGCAAATTGTGGTACAGAGTTGAGAGATTTAGCAGAACGAAAGTGTTCGTCGGAAATTGCTATGCGATCACGCCGGAGAGGCTAATCGGAAGATTCCCCGGCGTCAAATCCCTAACCCTAAAGGGAAAGCCTCATTTCGCCGATTTCAATTTGGTCCCTCATGATTGGGGCGGCTATGTCTACCCTTGGATCCAAGCCTTTGCCAAGCGTCGGATTGCCCTCGAGGAGCTCCGTTTGAAGCGGATGGTCGTCACTGACGACAGTCTTGAGCTCCTTTCCCGATCCTTCTCCAATTTCAAGTCGCTGTTCCTCTTCAGCTGTGAGGGATTCACCACCAATGGCCTTGCTGCCATTGCTGCCAATTGCAGGTTTCTGAGGGAGCTGGACCTGCAAGAGAATGAAATTGATGACCATAGTAATTACTGGCTTAGCTGCTTTCCAGAGAACTGCACATCGCTTGTCTCCCTGAATTTTGCTTGCCTCAGAGGAGAAGTAAATCTGGGTGCTCTTGAGAGGCTTGTGGCGAGATCTCCAAACCTCAAGAGTTTGAGGCTGAACCGTGCGGTGCCTCTTGAAACCTTGCATAGTATATTGGAACGCGCTCCGCAACTTGTGGACTTGGGCACCGGGTCTTACGTTCACGATCGAGATTCCGAAATCTACGACAATCTTAAGAACACCATTCTGAAATGCAAATCGATCAGGAGTTTATCTGGATTTTTAGATGTTTCTCCTCGCTGCCTGGCCTCCATTTACCCCATTTGCTCAAATTTGACATCTTTGAACCTGAGCTATGCTCCTGGGCTTCATGGCAGTGAGCTCATAAAGGTTATTCGGTATTGCGAGAAGCTTCAGCGCTTATGGATTCTGGATGGTATTGGAGACAAAGGACTCGAAGTTGTTGCTTCAACTTGTAAAGAACTGCAGGAATTGAGGGTTTTCCCATCTGATCTCTCTGGGGCTGGTAATGTTGCTGTCACAGAAGAAGGTTTGGTTGCTATATCGATGGGTTGCCCGAAACTTCATTCGATACTATACTTCTGCCATCAGATGACAAATGCTGCCCTTGTAACGGTAGCAAAGAACAACCCGAACTTCATACGCTTCAGGTTGTGCATCCTCAACCCCACGAAACCGGACCCTGTAACTGGGAATGCACTGGATGAAGGTTTTGGGGCGATTGTCCAAGCGTGCAAAGGTCTGAGACGTTTATCGCTCTCAGGCCTTCTTACAGATCAGGTCTTCTACTACATCGGTGAATACGCAAAGCATCTCGAAATGCTTTCTTTAGCATTTGCTGGGGACAGTGACAAGGGAATGATTCACGTATTGAACGGTTGCAAGAAACTTCGCAAGCTCGAGATCATGGACAGCCCGTTCGGCGACATGGCACTTCTGCAGGACGTCGGGAAGTATGAAACAATGCGATCCCTTTGGATGTCGTCCTGCCAGATTACTCTTGGTGGCTGCAAGACGCTAGCGAAGAAGATGCCGAGGCTGAACGTGGAGATCATCGACGAGGACGATCAGTCGGAGTTCTGCCGTGATGATGGGCAGAAAGTAGGGAAAATGTATCTGTACCGTACATTAGTAGGGCCGAGGAAAGATGCACCCAAGTTTGTGTGGACATTGTAG

Protein sequence

MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRTKVFVGNCYAITPERLIGRFPGVKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRIALEELRLKRMVVTDDSLELLSRSFSNFKSLFLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPENCTSLVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHSILERAPQLVDLGTGSYVHDRDSEIYDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGLHGSELIKVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLVAISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILNPTKPDPVTGNALDEGFGAIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKKLRKLEIMDSPFGDMALLQDVGKYETMRSLWMSSCQITLGGCKTLAKKMPRLNVEIIDEDDQSEFCRDDGQKVGKMYLYRTLVGPRKDAPKFVWTL
Homology
BLAST of Lcy09g021070 vs. ExPASy Swiss-Prot
Match: Q9LW29 (Protein AUXIN SIGNALING F-BOX 2 OS=Arabidopsis thaliana OX=3702 GN=AFB2 PE=1 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 2.6e-263
Identity = 437/575 (76.00%), Postives = 492/575 (85.57%), Query Frame = 0

Query: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRTKVFVGNCYAITPERLIGRF 60
           MNYFPDEV+EHVFD+VTSH+DRNA+SLVCK WY++ER+SR KVF+GNCYAI PERL+ RF
Sbjct: 1   MNYFPDEVIEHVFDFVTSHKDRNAISLVCKSWYKIERYSRQKVFIGNCYAINPERLLRRF 60

Query: 61  PGVKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRIALEELRLKRMVVTDDSLELL 120
           P +KSLTLKGKPHFADFNLVPH+WGG+V PWI+A A+ R+ LEELRLKRMVVTD+SLELL
Sbjct: 61  PCLKSLTLKGKPHFADFNLVPHEWGGFVLPWIEALARSRVGLEELRLKRMVVTDESLELL 120

Query: 121 SRSFSNFKSLFLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPENCTS 180
           SRSF NFKSL L SCEGFTT+GLA+IAANCR LR+LDLQENEIDDH   WLSCFP+ CT+
Sbjct: 121 SRSFVNFKSLVLVSCEGFTTDGLASIAANCRHLRDLDLQENEIDDHRGQWLSCFPDTCTT 180

Query: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHSILERAPQLVDLGTGSY 240
           LV+LNFACL GE NL ALERLVARSPNLKSL+LNRAVPL+ L  ++  APQ+VDLG GSY
Sbjct: 181 LVTLNFACLEGETNLVALERLVARSPNLKSLKLNRAVPLDALARLMACAPQIVDLGVGSY 240

Query: 241 VHDRDSEIYDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGLHGS 300
            +D DSE Y  L   I KC S+RSLSGFL+ +P CL++ +PIC NLTSLNLSYA  +HGS
Sbjct: 241 ENDPDSESYLKLMAVIKKCTSLRSLSGFLEAAPHCLSAFHPICHNLTSLNLSYAAEIHGS 300

Query: 301 ELIKVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV 360
            LIK+I++C+KLQRLWILD IGDKGLEVVASTCKELQELRVFPSDL G GN AVTEEGLV
Sbjct: 301 HLIKLIQHCKKLQRLWILDSIGDKGLEVVASTCKELQELRVFPSDLLGGGNTAVTEEGLV 360

Query: 361 AISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILNPTKPDPVTGNALDEGFG 420
           AIS GCPKLHSILYFC QMTNAALVTVAKN PNFIRFRLCIL P KPD VT   LDEGFG
Sbjct: 361 AISAGCPKLHSILYFCQQMTNAALVTVAKNCPNFIRFRLCILEPNKPDHVTSQPLDEGFG 420

Query: 421 AIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKKLRK 480
           AIV+ACK LRRLSLSGLLTDQVF YIG YA  LEMLS+AFAGD+DKGM++VLNGCKK++K
Sbjct: 421 AIVKACKSLRRLSLSGLLTDQVFLYIGMYANQLEMLSIAFAGDTDKGMLYVLNGCKKMKK 480

Query: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCQITLGGCKTLAKKMPRLNVEIIDEDDQS- 540
           LEI DSPFGD ALL DV KYETMRSLWMSSC++TL GCK LA+K P LNVEII+E+D + 
Sbjct: 481 LEIRDSPFGDTALLADVSKYETMRSLWMSSCEVTLSGCKRLAEKAPWLNVEIINENDNNR 540

Query: 541 --EFCRDDGQKVGKMYLYRTLVGPRKDAPKFVWTL 573
             E   +  QKV K+YLYRT+VG R DAP FVW L
Sbjct: 541 MEENGHEGRQKVDKLYLYRTVVGTRMDAPPFVWIL 575

BLAST of Lcy09g021070 vs. ExPASy Swiss-Prot
Match: Q9LPW7 (Protein AUXIN SIGNALING F-BOX 3 OS=Arabidopsis thaliana OX=3702 GN=AFB3 PE=1 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 5.4e-253
Identity = 417/574 (72.65%), Postives = 491/574 (85.54%), Query Frame = 0

Query: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRTKVFVGNCYAITPERLIGRF 60
           MNYFPDEV+EHVFD+V SH+DRN++SLVCK W+++ERFSR +VF+GNCYAI PERLI RF
Sbjct: 1   MNYFPDEVIEHVFDFVASHKDRNSISLVCKSWHKIERFSRKEVFIGNCYAINPERLIRRF 60

Query: 61  PGVKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRIALEELRLKRMVVTDDSLELL 120
           P +KSLTLKGKPHFADFNLVPH+WGG+V+PWI+A A+ R+ LEELRLKRMVVTD+SL+LL
Sbjct: 61  PCLKSLTLKGKPHFADFNLVPHEWGGFVHPWIEALARSRVGLEELRLKRMVVTDESLDLL 120

Query: 121 SRSFSNFKSLFLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPENCTS 180
           SRSF+NFKSL L SCEGFTT+GLA+IAANCR LRELDLQENEIDDH   WL+CFP++CT+
Sbjct: 121 SRSFANFKSLVLVSCEGFTTDGLASIAANCRHLRELDLQENEIDDHRGQWLNCFPDSCTT 180

Query: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHSILERAPQLVDLGTGSY 240
           L+SLNFACL+GE N+ ALERLVARSPNLKSL+LNRAVPL+ L  ++  APQLVDLG GSY
Sbjct: 181 LMSLNFACLKGETNVAALERLVARSPNLKSLKLNRAVPLDALARLMSCAPQLVDLGVGSY 240

Query: 241 VHDRDSEIYDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGLHGS 300
            ++ D E +  L   I K  S+RSLSGFL+V+P CL + YPIC NL SLNLSYA  + G+
Sbjct: 241 ENEPDPESFAKLMTAIKKYTSLRSLSGFLEVAPLCLPAFYPICQNLISLNLSYAAEIQGN 300

Query: 301 ELIKVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGA--GNVAVTEEG 360
            LIK+I+ C++LQRLWILD IGDKGL VVA+TCKELQELRVFPSD+ G    N +VTE G
Sbjct: 301 HLIKLIQLCKRLQRLWILDSIGDKGLAVVAATCKELQELRVFPSDVHGEEDNNASVTEVG 360

Query: 361 LVAISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILNPTKPDPVTGNALDEG 420
           LVAIS GCPKLHSILYFC QMTNAAL+ VAKN PNFIRFRLCIL P KPD +T  +LDEG
Sbjct: 361 LVAISAGCPKLHSILYFCKQMTNAALIAVAKNCPNFIRFRLCILEPHKPDHITFQSLDEG 420

Query: 421 FGAIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKKL 480
           FGAIVQACKGLRRLS+SGLLTDQVF YIG YA+ LEMLS+AFAGD+DKGM++VLNGCKK+
Sbjct: 421 FGAIVQACKGLRRLSVSGLLTDQVFLYIGMYAEQLEMLSIAFAGDTDKGMLYVLNGCKKM 480

Query: 481 RKLEIMDSPFGDMALLQDVGKYETMRSLWMSSCQITLGGCKTLAKKMPRLNVEIIDEDDQ 540
           RKLEI DSPFG+ ALL DVG+YETMRSLWMSSC++TLGGCK LA+  PRLNVEII+E++ 
Sbjct: 481 RKLEIRDSPFGNAALLADVGRYETMRSLWMSSCEVTLGGCKRLAQNSPRLNVEIINENEN 540

Query: 541 S---EFCRDDGQKVGKMYLYRTLVGPRKDAPKFV 570
           +   +   D+ +KV K+YLYRT+VG RKDAP +V
Sbjct: 541 NGMEQNEEDEREKVDKLYLYRTVVGTRKDAPPYV 574

BLAST of Lcy09g021070 vs. ExPASy Swiss-Prot
Match: Q7XVM8 (Transport inhibitor response 1-like protein Os04g0395600 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0395600 PE=2 SV=1)

HSP 1 Score: 808.5 bits (2087), Expect = 4.8e-233
Identity = 388/572 (67.83%), Postives = 461/572 (80.59%), Query Frame = 0

Query: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRTKVFVGNCYAITPERLIGRF 60
           M YFP+EV+EH+F ++ + RDRN VSLVCK+WY +ER SR  VFVGNCYA+   R+  RF
Sbjct: 1   MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVGNCYAVRAGRVAARF 60

Query: 61  PGVKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRIALEELRLKRMVVTDDSLELL 120
           P V++LT+KGKPHFADFNLVP DWGGY  PWI+A A+    LEELR+KRMVV+D+SLELL
Sbjct: 61  PNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEELRMKRMVVSDESLELL 120

Query: 121 SRSFSNFKSLFLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPENCTS 180
           +RSF  F++L L SCEGF+T+GLAA+A++C+ LRELDLQENE++D    WLSCFP++CTS
Sbjct: 121 ARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVEDRGPRWLSCFPDSCTS 180

Query: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHSILERAPQLVDLGTGSY 240
           LVSLNFAC++GEVN G+LERLV+RSPNL+SLRLNR+V ++TL  IL R P L DLGTG+ 
Sbjct: 181 LVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLAKILLRTPNLEDLGTGNL 240

Query: 241 VHDRDSEIYDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGLHGS 300
             D  +E Y  L + + KCK +RSLSGF D SP CL+ IYP+C+ LT LNLSYAP L  S
Sbjct: 241 TDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPLCAQLTGLNLSYAPTLDAS 300

Query: 301 ELIKVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV 360
           +L K+I  C KLQRLW+LD I DKGL+VVAS+CK+LQELRVFPSD   AG  AVTEEGLV
Sbjct: 301 DLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRVFPSDFYVAGYSAVTEEGLV 360

Query: 361 AISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILNPTKPDPVTGNALDEGFG 420
           A+S+GCPKL+S+LYFCHQMTNAALVTVAKN PNF RFRLCIL P KPD VT   LDEGFG
Sbjct: 361 AVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLCILEPGKPDVVTSQPLDEGFG 420

Query: 421 AIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKKLRK 480
           AIV+ CKGL+RLS+SGLLTD+VF YIG+YAK LEMLS+AFAGDSDKGM+HV+NGCK LRK
Sbjct: 421 AIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIAFAGDSDKGMMHVMNGCKNLRK 480

Query: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCQITLGGCKTLAKKMPRLNVEIIDEDDQS- 540
           LEI DSPFGD ALL +  +YETMRSLWMSSC +TL GC+ LA KMP LNVE+I+E D S 
Sbjct: 481 LEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGCQVLASKMPMLNVEVINERDGSN 540

Query: 541 --EFCRDDGQKVGKMYLYRTLVGPRKDAPKFV 570
             E    D  KV K+Y+YRT  G R DAP FV
Sbjct: 541 EMEENHGDLPKVEKLYVYRTTAGARDDAPNFV 572

BLAST of Lcy09g021070 vs. ExPASy Swiss-Prot
Match: Q570C0 (Protein TRANSPORT INHIBITOR RESPONSE 1 OS=Arabidopsis thaliana OX=3702 GN=TIR1 PE=1 SV=2)

HSP 1 Score: 734.2 bits (1894), Expect = 1.1e-210
Identity = 354/570 (62.11%), Postives = 437/570 (76.67%), Query Frame = 0

Query: 4   FPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRTKVFVGNCYAITPERLIGRFPGV 63
           FP+EVLEHVF ++   +DRN+VSLVCK WY +ER+ R KVF+GNCYA++P  +I RFP V
Sbjct: 9   FPEEVLEHVFSFIQLDKDRNSVSLVCKSWYEIERWCRRKVFIGNCYAVSPATVIRRFPKV 68

Query: 64  KSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRIALEELRLKRMVVTDDSLELLSRS 123
           +S+ LKGKPHFADFNLVP  WGGYVYPWI+A +     LEE+RLKRMVVTDD LEL+++S
Sbjct: 69  RSVELKGKPHFADFNLVPDGWGGYVYPWIEAMSSSYTWLEEIRLKRMVVTDDCLELIAKS 128

Query: 124 FSNFKSLFLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPENCTSLVS 183
           F NFK L L SCEGF+T+GLAAIAA CR L+ELDL+E+++DD S +WLS FP+  TSLVS
Sbjct: 129 FKNFKVLVLSSCEGFSTDGLAAIAATCRNLKELDLRESDVDDVSGHWLSHFPDTYTSLVS 188

Query: 184 LNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHSILERAPQLVDLGTGSYVHD 243
           LN +CL  EV+  ALERLV R PNLKSL+LNRAVPLE L ++L+RAPQL +LGTG Y  +
Sbjct: 189 LNISCLASEVSFSALERLVTRCPNLKSLKLNRAVPLEKLATLLQRAPQLEELGTGGYTAE 248

Query: 244 RDSEIYDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGLHGSELI 303
              ++Y  L   +  CK +R LSGF D  P  L ++Y +CS LT+LNLSYA  +   +L+
Sbjct: 249 VRPDVYSGLSVALSGCKELRCLSGFWDAVPAYLPAVYSVCSRLTTLNLSYAT-VQSYDLV 308

Query: 304 KVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSD-LSGAGNVAVTEEGLVAI 363
           K++  C KLQRLW+LD I D GLEV+ASTCK+L+ELRVFPS+      NVA+TE+GLV++
Sbjct: 309 KLLCQCPKLQRLWVLDYIEDAGLEVLASTCKDLRELRVFPSEPFVMEPNVALTEQGLVSV 368

Query: 364 SMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILNPTKPDPVTGNALDEGFGAI 423
           SMGCPKL S+LYFC QMTNAAL+T+A+N PN  RFRLCI+ P  PD +T   LD GFGAI
Sbjct: 369 SMGCPKLESVLYFCRQMTNAALITIARNRPNMTRFRLCIIEPKAPDYLTLEPLDIGFGAI 428

Query: 424 VQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKKLRKLE 483
           V+ CK LRRLSLSGLLTD+VF YIG YAK +EMLS+AFAGDSD GM HVL+GC  LRKLE
Sbjct: 429 VEHCKDLRRLSLSGLLTDKVFEYIGTYAKKMEMLSVAFAGDSDLGMHHVLSGCDSLRKLE 488

Query: 484 IMDSPFGDMALLQDVGKYETMRSLWMSSCQITLGGCKTLAKKMPRLNVEIIDEDDQSEFC 543
           I D PFGD ALL +  K ETMRSLWMSSC ++ G CK L +KMP+LNVE+IDE    +  
Sbjct: 489 IRDCPFGDKALLANASKLETMRSLWMSSCSVSFGACKLLGQKMPKLNVEVIDERGAPD-S 548

Query: 544 RDDGQKVGKMYLYRTLVGPRKDAPKFVWTL 573
           R +   V ++++YRT+ GPR D P FVW +
Sbjct: 549 RPESCPVERVFIYRTVAGPRFDMPGFVWNM 576

BLAST of Lcy09g021070 vs. ExPASy Swiss-Prot
Match: Q2R3K5 (Transport inhibitor response 1-like protein Os11g0515500 OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g0515500 PE=2 SV=1)

HSP 1 Score: 682.2 bits (1759), Expect = 5.1e-195
Identity = 340/577 (58.93%), Postives = 423/577 (73.31%), Query Frame = 0

Query: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRTKVFVGNCYAITPERLIGRF 60
           M +FP+EV+EH+  ++ SHRDRNAVSLVC+ WYRVER SR  V V NCYA  PER+  RF
Sbjct: 1   MVFFPEEVVEHILGFLASHRDRNAVSLVCREWYRVERLSRRSVLVRNCYAARPERVHARF 60

Query: 61  PGVKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRIALEELRLKRMVVTDDSLELL 120
           PG++SL++KG+P F     VP  WG    PW+ A       LEELRLKRMVVTD  L+LL
Sbjct: 61  PGLRSLSVKGRPRF-----VPAGWGAAARPWVAACVAACPGLEELRLKRMVVTDGCLKLL 120

Query: 121 SRSFSNFKSLFLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPENCTS 180
           + SF N KSL L  C+GF+T+GLA +A NCRF++ELDLQE+ ++D  + WL CFP+  T 
Sbjct: 121 ACSFPNLKSLVLVGCQGFSTDGLATVATNCRFMKELDLQESLVEDRDSRWLGCFPKPSTL 180

Query: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHSILERAPQLVDLGTGSY 240
           L SLNF+CL GEVN  ALE LVARSPNL+SLRLNR+VPL+ L  IL R P+LVDL TGS+
Sbjct: 181 LESLNFSCLTGEVNSPALEILVARSPNLRSLRLNRSVPLDVLARILCRRPRLVDLCTGSF 240

Query: 241 VHDRDSEIYDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGLHGS 300
           V       Y  L N+   C  ++SLSGF D +   +  I P+C NLT LNLS AP +  +
Sbjct: 241 VRGNIVGAYAGLFNSFQHCSLLKSLSGFWDATSLFIPVIAPVCKNLTCLNLSSAPMVRSA 300

Query: 301 ELIKVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV 360
            LI+ I  C+KLQ+LW+LD IGD+GL++VAS+C +LQELRVFP++ +   +  VTEEGLV
Sbjct: 301 YLIEFICQCKKLQQLWVLDHIGDEGLKIVASSCIQLQELRVFPANANARAS-TVTEEGLV 360

Query: 361 AISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILNPTKPDPVTGNALDEGFG 420
           AIS GC KL S+LYFC +MTN+AL+TVAKN P F  FRLC+L+P   D VTG  LDEG+G
Sbjct: 361 AISAGCNKLQSVLYFCQRMTNSALITVAKNCPRFTSFRLCVLDPGSADAVTGQPLDEGYG 420

Query: 421 AIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKKLRK 480
           AIVQ+CKGLRRL LSGLLTD VF YIG YA+ LEMLS+AFAGD+D GM +VLNGCK L+K
Sbjct: 421 AIVQSCKGLRRLCLSGLLTDTVFLYIGMYAERLEMLSVAFAGDTDDGMTYVLNGCKNLKK 480

Query: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCQITLGGCKTLAKKMPRLNVEI------ID 540
           LEI DSPFGD ALL  + +YE MRSLW+SSC +TLGGCK+LA  M  LN+E+      I+
Sbjct: 481 LEIRDSPFGDSALLAGMHQYEAMRSLWLSSCNVTLGGCKSLAASMANLNIEVMNRAASIN 540

Query: 541 EDDQSEFCRDDGQKVGKMYLYRTLVGPRKDAPKFVWT 572
           E D +    +D +KV K+Y+YRT+ GPR DAP+F+ T
Sbjct: 541 EADNA----NDAKKVKKLYIYRTVAGPRGDAPEFIST 567

BLAST of Lcy09g021070 vs. ExPASy TrEMBL
Match: A0A6J1DQR9 (protein AUXIN SIGNALING F-BOX 2-like OS=Momordica charantia OX=3673 GN=LOC111023376 PE=4 SV=1)

HSP 1 Score: 1122.5 bits (2902), Expect = 0.0e+00
Identity = 542/572 (94.76%), Postives = 558/572 (97.55%), Query Frame = 0

Query: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRTKVFVGNCYAITPERLIGRF 60
           MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSR KVFVGNCYAITPERLIGRF
Sbjct: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRQKVFVGNCYAITPERLIGRF 60

Query: 61  PGVKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRIALEELRLKRMVVTDDSLELL 120
           P VKSLTLKGKPHFADFNLVPHDWGG+VYPWIQ FAKRRIALEELRLKRMVV+DDSLELL
Sbjct: 61  PAVKSLTLKGKPHFADFNLVPHDWGGFVYPWIQVFAKRRIALEELRLKRMVVSDDSLELL 120

Query: 121 SRSFSNFKSLFLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPENCTS 180
           SRSF +FKSLFLFSCEGFTTNGLAAIA+NCR+LRELDLQENEIDDHSNYWLSCFPENCTS
Sbjct: 121 SRSFPHFKSLFLFSCEGFTTNGLAAIASNCRYLRELDLQENEIDDHSNYWLSCFPENCTS 180

Query: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHSILERAPQLVDLGTGSY 240
           LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLH+IL RAPQLVDLGTGSY
Sbjct: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHNILIRAPQLVDLGTGSY 240

Query: 241 VHDRDSEIYDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGLHGS 300
           VHDRDSE YDNLKNTILKCKSIRSLSGFLDVSPRCL SIYPICSNLTSLNLSYAPGLHGS
Sbjct: 241 VHDRDSETYDNLKNTILKCKSIRSLSGFLDVSPRCLPSIYPICSNLTSLNLSYAPGLHGS 300

Query: 301 ELIKVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV 360
           EL+KVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV
Sbjct: 301 ELVKVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV 360

Query: 361 AISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILNPTKPDPVTGNALDEGFG 420
           AISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCIL+PTK DPVT   LDEGFG
Sbjct: 361 AISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILDPTKADPVTNQPLDEGFG 420

Query: 421 AIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKKLRK 480
           AIVQACKGLRRLSLSGLL+DQVFYYIGEYAKHLEMLSLAFAG +DKGMIHVLNGCKKLRK
Sbjct: 421 AIVQACKGLRRLSLSGLLSDQVFYYIGEYAKHLEMLSLAFAGGTDKGMIHVLNGCKKLRK 480

Query: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCQITLGGCKTLAKKMPRLNVEIIDEDDQSE 540
           LEIMDSPFGDMALLQDVGKYETMRSLWMSSC+ITLGGCKTLAKKMPRLNVEII+E+D SE
Sbjct: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCEITLGGCKTLAKKMPRLNVEIINENDPSE 540

Query: 541 FCRDDGQKVGKMYLYRTLVGPRKDAPKFVWTL 573
           F RDDG++VGKMYLYRTLVGPRKDAPKFVWT+
Sbjct: 541 FGRDDGKRVGKMYLYRTLVGPRKDAPKFVWTV 572

BLAST of Lcy09g021070 vs. ExPASy TrEMBL
Match: A0A6J1J852 (protein AUXIN SIGNALING F-BOX 2-like OS=Cucurbita maxima OX=3661 GN=LOC111482144 PE=4 SV=1)

HSP 1 Score: 1119.8 bits (2895), Expect = 0.0e+00
Identity = 541/572 (94.58%), Postives = 559/572 (97.73%), Query Frame = 0

Query: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRTKVFVGNCYAITPERLIGRF 60
           MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWY+VERFSR KVFVGNCY+ITPERLIGRF
Sbjct: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYKVERFSRQKVFVGNCYSITPERLIGRF 60

Query: 61  PGVKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRIALEELRLKRMVVTDDSLELL 120
           P VKSLTLKGKPHFADFNLVPHDWGGYV+PWIQAFAKRRIALEELRLKRMVVTD+SLELL
Sbjct: 61  PCVKSLTLKGKPHFADFNLVPHDWGGYVHPWIQAFAKRRIALEELRLKRMVVTDESLELL 120

Query: 121 SRSFSNFKSLFLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPENCTS 180
           SRSF NFKSL LFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPE+CTS
Sbjct: 121 SRSFPNFKSLLLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPESCTS 180

Query: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHSILERAPQLVDLGTGSY 240
           LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETL +IL RAPQLVDLGTGSY
Sbjct: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLQNILVRAPQLVDLGTGSY 240

Query: 241 VHDRDSEIYDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGLHGS 300
           VHD+DSEIYDNLKNTILKCKSIRSLSGFLDVSP CLASIYPICSNLTSLNLSYAPGLHG 
Sbjct: 241 VHDQDSEIYDNLKNTILKCKSIRSLSGFLDVSPSCLASIYPICSNLTSLNLSYAPGLHGI 300

Query: 301 ELIKVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV 360
           EL+KV+RYC+KLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV
Sbjct: 301 ELMKVVRYCDKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV 360

Query: 361 AISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILNPTKPDPVTGNALDEGFG 420
           AISMGCPKLHSILYFCHQMTNAALV VAKNNPNFIRFRLCIL+PTKPDPVTG+ALDEGFG
Sbjct: 361 AISMGCPKLHSILYFCHQMTNAALVAVAKNNPNFIRFRLCILDPTKPDPVTGHALDEGFG 420

Query: 421 AIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKKLRK 480
           AIVQACKGLRRLSLSG LTD+VFYYIG+YAKHLEMLSLAFAGDSDKGMIH+LNGCKKLRK
Sbjct: 421 AIVQACKGLRRLSLSGHLTDRVFYYIGKYAKHLEMLSLAFAGDSDKGMIHILNGCKKLRK 480

Query: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCQITLGGCKTLAKKMPRLNVEIIDEDDQSE 540
           LEIMDSPFGDMALLQDVGKYETMRSLWMSSC+ITLGGCKTLAKKMPRLNVEII+E+D SE
Sbjct: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCEITLGGCKTLAKKMPRLNVEIINENDLSE 540

Query: 541 FCRDDGQKVGKMYLYRTLVGPRKDAPKFVWTL 573
             RDDGQKVGKMYLYRTLVGPRKDAPKFVWTL
Sbjct: 541 LSRDDGQKVGKMYLYRTLVGPRKDAPKFVWTL 572

BLAST of Lcy09g021070 vs. ExPASy TrEMBL
Match: A0A6J1F8M3 (protein AUXIN SIGNALING F-BOX 2-like OS=Cucurbita moschata OX=3662 GN=LOC111441855 PE=4 SV=1)

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 541/572 (94.58%), Postives = 558/572 (97.55%), Query Frame = 0

Query: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRTKVFVGNCYAITPERLIGRF 60
           MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSR KVFVGNCY+ITPERLIGRF
Sbjct: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRQKVFVGNCYSITPERLIGRF 60

Query: 61  PGVKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRIALEELRLKRMVVTDDSLELL 120
           P VKSLTLKGKPHFADFNLVPHDWGGYV+PWIQAFAKRRIALEELRLKRMVVTD+SLELL
Sbjct: 61  PCVKSLTLKGKPHFADFNLVPHDWGGYVHPWIQAFAKRRIALEELRLKRMVVTDESLELL 120

Query: 121 SRSFSNFKSLFLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPENCTS 180
           SRSF NFKSL LFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPE+CTS
Sbjct: 121 SRSFPNFKSLLLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPESCTS 180

Query: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHSILERAPQLVDLGTGSY 240
           LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETL +IL RAPQLVDLGTGSY
Sbjct: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLQNILVRAPQLVDLGTGSY 240

Query: 241 VHDRDSEIYDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGLHGS 300
           VHD+DSEIYDNLKNTILKCKSIRSLSGFLDVSP CLASIYPICSNLTSLNLSYAPGLHG 
Sbjct: 241 VHDQDSEIYDNLKNTILKCKSIRSLSGFLDVSPSCLASIYPICSNLTSLNLSYAPGLHGI 300

Query: 301 ELIKVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV 360
           EL+KV+RYC+KLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV
Sbjct: 301 ELMKVVRYCDKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV 360

Query: 361 AISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILNPTKPDPVTGNALDEGFG 420
           AISMGCPKLHSILYFCHQMTNAALV VAKNNPNFIRFRLCIL+PTKPDPVTG+ALDEGFG
Sbjct: 361 AISMGCPKLHSILYFCHQMTNAALVAVAKNNPNFIRFRLCILDPTKPDPVTGHALDEGFG 420

Query: 421 AIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKKLRK 480
           AIVQACKGLRRLSLSG LTD+VFYYIG+YAKHLEMLSLAFAGDSDKGMIH+LNGCKKLRK
Sbjct: 421 AIVQACKGLRRLSLSGHLTDRVFYYIGKYAKHLEMLSLAFAGDSDKGMIHILNGCKKLRK 480

Query: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCQITLGGCKTLAKKMPRLNVEIIDEDDQSE 540
           LEIMDSPFGDMALLQDVGKYETMRSLWMSSC+ITLGGCKTLAKKMPRLNVEII+E+D  E
Sbjct: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCEITLGGCKTLAKKMPRLNVEIINENDLLE 540

Query: 541 FCRDDGQKVGKMYLYRTLVGPRKDAPKFVWTL 573
            CRDD QKVGKMYLYRTLVGPRKDAPKFVWTL
Sbjct: 541 LCRDDEQKVGKMYLYRTLVGPRKDAPKFVWTL 572

BLAST of Lcy09g021070 vs. ExPASy TrEMBL
Match: A0A5D3DU60 (Protein AUXIN SIGNALING F-BOX 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold236G001160 PE=4 SV=1)

HSP 1 Score: 1108.2 bits (2865), Expect = 0.0e+00
Identity = 540/587 (91.99%), Postives = 558/587 (95.06%), Query Frame = 0

Query: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRTKVFVGNCYAITPERLIGRF 60
           MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRV+RFSR KVFVGNCY+ITPER+IGRF
Sbjct: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVDRFSRQKVFVGNCYSITPERVIGRF 60

Query: 61  PGVKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRIALEELRLKRMVVTDDSLELL 120
           P VKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRI+LEELRLKRMVVTDDSLELL
Sbjct: 61  PCVKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRISLEELRLKRMVVTDDSLELL 120

Query: 121 SRSFSNFKSLFLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPENCTS 180
           SRSF NFKSL LFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPE+CTS
Sbjct: 121 SRSFPNFKSLLLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPESCTS 180

Query: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHSILERAPQLVDLGTGSY 240
           LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVP+ETL +IL  APQLVDLGTGSY
Sbjct: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPIETLQNILANAPQLVDLGTGSY 240

Query: 241 VHDRDSEIYDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGLHGS 300
           VHDRDSEIYDNLKNT+LKCKSIRSLSGFLDVSP CLASIYPICSNLTSLNLSYAPGLHG+
Sbjct: 241 VHDRDSEIYDNLKNTLLKCKSIRSLSGFLDVSPCCLASIYPICSNLTSLNLSYAPGLHGN 300

Query: 301 ELIKVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV 360
           ELIKVI+YCE+LQRLWILDGIGDKGLEVVAS+C ELQELRVFPSDLSGAGNVAVTEEGLV
Sbjct: 301 ELIKVIQYCERLQRLWILDGIGDKGLEVVASSCNELQELRVFPSDLSGAGNVAVTEEGLV 360

Query: 361 AISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILNPTKPDPVTGNALDEGFG 420
           AIS GCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCIL+PTKPDPVTG  LDEGFG
Sbjct: 361 AISKGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILDPTKPDPVTGYPLDEGFG 420

Query: 421 AIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKKLRK 480
           AIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAG+SDKGMIHVL+GCKKLRK
Sbjct: 421 AIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGNSDKGMIHVLSGCKKLRK 480

Query: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCQITLGGCKTLAKKMPRLNVEIIDEDD--- 540
           LEIMDSPFGDMALLQDVGKYETMRSLWMSSC+ITLGGCKTLAKKMPRLNVEII+E+D   
Sbjct: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCEITLGGCKTLAKKMPRLNVEIINENDQLG 540

Query: 541 ------------QSEFCRDDGQKVGKMYLYRTLVGPRKDAPKFVWTL 573
                       QSE CRDD QKVGKMYLYRTLVGPRKDAPKFVWTL
Sbjct: 541 FCRNVDMINENGQSEVCRDDEQKVGKMYLYRTLVGPRKDAPKFVWTL 587

BLAST of Lcy09g021070 vs. ExPASy TrEMBL
Match: A0A1S3BMP8 (protein AUXIN SIGNALING F-BOX 2-like OS=Cucumis melo OX=3656 GN=LOC103491723 PE=4 SV=1)

HSP 1 Score: 1107.0 bits (2862), Expect = 0.0e+00
Identity = 539/587 (91.82%), Postives = 558/587 (95.06%), Query Frame = 0

Query: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRTKVFVGNCYAITPERLIGRF 60
           MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRV+RFSR KVFVGNCY+ITPER+IGRF
Sbjct: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVDRFSRQKVFVGNCYSITPERVIGRF 60

Query: 61  PGVKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRIALEELRLKRMVVTDDSLELL 120
           P VKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRI+LEELRLKRMVVTDDSLELL
Sbjct: 61  PCVKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRISLEELRLKRMVVTDDSLELL 120

Query: 121 SRSFSNFKSLFLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPENCTS 180
           SRSF NFKSL LFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYW+SCFPE+CTS
Sbjct: 121 SRSFPNFKSLLLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWVSCFPESCTS 180

Query: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHSILERAPQLVDLGTGSY 240
           LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVP+ETL +IL  APQLVDLGTGSY
Sbjct: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPIETLQNILANAPQLVDLGTGSY 240

Query: 241 VHDRDSEIYDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGLHGS 300
           VHDRDSEIYDNLKNT+LKCKSIRSLSGFLDVSP CLASIYPICSNLTSLNLSYAPGLHG+
Sbjct: 241 VHDRDSEIYDNLKNTLLKCKSIRSLSGFLDVSPCCLASIYPICSNLTSLNLSYAPGLHGN 300

Query: 301 ELIKVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV 360
           ELIKVI+YCE+LQRLWILDGIGDKGLEVVAS+C ELQELRVFPSDLSGAGNVAVTEEGLV
Sbjct: 301 ELIKVIQYCERLQRLWILDGIGDKGLEVVASSCNELQELRVFPSDLSGAGNVAVTEEGLV 360

Query: 361 AISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILNPTKPDPVTGNALDEGFG 420
           AIS GCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCIL+PTKPDPVTG  LDEGFG
Sbjct: 361 AISKGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILDPTKPDPVTGYPLDEGFG 420

Query: 421 AIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKKLRK 480
           AIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAG+SDKGMIHVL+GCKKLRK
Sbjct: 421 AIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGNSDKGMIHVLSGCKKLRK 480

Query: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCQITLGGCKTLAKKMPRLNVEIIDEDD--- 540
           LEIMDSPFGDMALLQDVGKYETMRSLWMSSC+ITLGGCKTLAKKMPRLNVEII+E+D   
Sbjct: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCEITLGGCKTLAKKMPRLNVEIINENDQLG 540

Query: 541 ------------QSEFCRDDGQKVGKMYLYRTLVGPRKDAPKFVWTL 573
                       QSE CRDD QKVGKMYLYRTLVGPRKDAPKFVWTL
Sbjct: 541 FCRNVDMINENGQSEVCRDDEQKVGKMYLYRTLVGPRKDAPKFVWTL 587

BLAST of Lcy09g021070 vs. NCBI nr
Match: XP_038893828.1 (protein AUXIN SIGNALING F-BOX 2-like [Benincasa hispida])

HSP 1 Score: 1144.8 bits (2960), Expect = 0.0e+00
Identity = 554/572 (96.85%), Postives = 563/572 (98.43%), Query Frame = 0

Query: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRTKVFVGNCYAITPERLIGRF 60
           MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSR KVFVGNCY+ITPERLIGRF
Sbjct: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRQKVFVGNCYSITPERLIGRF 60

Query: 61  PGVKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRIALEELRLKRMVVTDDSLELL 120
           P VKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRI+LEELRLKRMVVTDDSLELL
Sbjct: 61  PCVKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRISLEELRLKRMVVTDDSLELL 120

Query: 121 SRSFSNFKSLFLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPENCTS 180
           SRSF NFKSL LFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPE+CTS
Sbjct: 121 SRSFPNFKSLLLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPESCTS 180

Query: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHSILERAPQLVDLGTGSY 240
           LV LNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETL +IL RAPQLVDLGTGSY
Sbjct: 181 LVCLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLQNILARAPQLVDLGTGSY 240

Query: 241 VHDRDSEIYDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGLHGS 300
           VHDRDSE+YDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGLHG+
Sbjct: 241 VHDRDSEMYDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGLHGN 300

Query: 301 ELIKVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV 360
           ELIKVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSG GNVAVTEEGLV
Sbjct: 301 ELIKVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGVGNVAVTEEGLV 360

Query: 361 AISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILNPTKPDPVTGNALDEGFG 420
           AISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCIL+PTKPDPVTGNALDEGFG
Sbjct: 361 AISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILDPTKPDPVTGNALDEGFG 420

Query: 421 AIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKKLRK 480
           AIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKKLRK
Sbjct: 421 AIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKKLRK 480

Query: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCQITLGGCKTLAKKMPRLNVEIIDEDDQSE 540
           LEIMDSPFGDMALLQDVGKYETMRSLWMSSC ITLGGCKTLAKKMPRLNVEIIDE++QSE
Sbjct: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCNITLGGCKTLAKKMPRLNVEIIDENNQSE 540

Query: 541 FCRDDGQKVGKMYLYRTLVGPRKDAPKFVWTL 573
           FCRDDGQKVGKMYLYRTLVGPRKDAPKFVWTL
Sbjct: 541 FCRDDGQKVGKMYLYRTLVGPRKDAPKFVWTL 572

BLAST of Lcy09g021070 vs. NCBI nr
Match: XP_023527075.1 (protein AUXIN SIGNALING F-BOX 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1123.6 bits (2905), Expect = 0.0e+00
Identity = 542/572 (94.76%), Postives = 560/572 (97.90%), Query Frame = 0

Query: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRTKVFVGNCYAITPERLIGRF 60
           MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSR KVFVGNCY+ITPERLIGRF
Sbjct: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRQKVFVGNCYSITPERLIGRF 60

Query: 61  PGVKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRIALEELRLKRMVVTDDSLELL 120
           P VKSLTLKGKPHFADFNLVPHDWGGYV+PWIQAFAKRRI+LEELRLKRMVVTD+SLELL
Sbjct: 61  PCVKSLTLKGKPHFADFNLVPHDWGGYVHPWIQAFAKRRISLEELRLKRMVVTDESLELL 120

Query: 121 SRSFSNFKSLFLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPENCTS 180
           SRSF NFKSL LFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPE+CTS
Sbjct: 121 SRSFPNFKSLLLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPESCTS 180

Query: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHSILERAPQLVDLGTGSY 240
           LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETL +IL RAPQLVDLGTGSY
Sbjct: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLQNILVRAPQLVDLGTGSY 240

Query: 241 VHDRDSEIYDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGLHGS 300
           VHD+DSEIYDNLKNTILKCKSIRSLSGFLDVSP CLASIYPICSNLTSLNLSYAPGLHG 
Sbjct: 241 VHDQDSEIYDNLKNTILKCKSIRSLSGFLDVSPSCLASIYPICSNLTSLNLSYAPGLHGI 300

Query: 301 ELIKVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV 360
           EL+KV+RYC+KLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV
Sbjct: 301 ELMKVVRYCDKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV 360

Query: 361 AISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILNPTKPDPVTGNALDEGFG 420
           AISMGCPKLHSILYFCHQMTNAALV VAKNNPNFIRFRLCIL+PTKPDPVTG+ALDEGFG
Sbjct: 361 AISMGCPKLHSILYFCHQMTNAALVAVAKNNPNFIRFRLCILDPTKPDPVTGHALDEGFG 420

Query: 421 AIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKKLRK 480
           AIVQACKGLRRLSLSG LTD+VFYYIG+YAKHLEMLSLAFAGDSDKGMIH+LNGCKKLRK
Sbjct: 421 AIVQACKGLRRLSLSGHLTDRVFYYIGKYAKHLEMLSLAFAGDSDKGMIHILNGCKKLRK 480

Query: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCQITLGGCKTLAKKMPRLNVEIIDEDDQSE 540
           LEIMDSPFGDMALLQDVGKYETMRSLWMSSC+ITLGGCKTLAKKMPRLNVEII+E+D SE
Sbjct: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCEITLGGCKTLAKKMPRLNVEIINENDLSE 540

Query: 541 FCRDDGQKVGKMYLYRTLVGPRKDAPKFVWTL 573
            CRDDGQKVGKMYLYRTLVGPRKDAPKFVWTL
Sbjct: 541 LCRDDGQKVGKMYLYRTLVGPRKDAPKFVWTL 572

BLAST of Lcy09g021070 vs. NCBI nr
Match: XP_022156493.1 (protein AUXIN SIGNALING F-BOX 2-like [Momordica charantia])

HSP 1 Score: 1122.5 bits (2902), Expect = 0.0e+00
Identity = 542/572 (94.76%), Postives = 558/572 (97.55%), Query Frame = 0

Query: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRTKVFVGNCYAITPERLIGRF 60
           MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSR KVFVGNCYAITPERLIGRF
Sbjct: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRQKVFVGNCYAITPERLIGRF 60

Query: 61  PGVKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRIALEELRLKRMVVTDDSLELL 120
           P VKSLTLKGKPHFADFNLVPHDWGG+VYPWIQ FAKRRIALEELRLKRMVV+DDSLELL
Sbjct: 61  PAVKSLTLKGKPHFADFNLVPHDWGGFVYPWIQVFAKRRIALEELRLKRMVVSDDSLELL 120

Query: 121 SRSFSNFKSLFLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPENCTS 180
           SRSF +FKSLFLFSCEGFTTNGLAAIA+NCR+LRELDLQENEIDDHSNYWLSCFPENCTS
Sbjct: 121 SRSFPHFKSLFLFSCEGFTTNGLAAIASNCRYLRELDLQENEIDDHSNYWLSCFPENCTS 180

Query: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHSILERAPQLVDLGTGSY 240
           LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLH+IL RAPQLVDLGTGSY
Sbjct: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHNILIRAPQLVDLGTGSY 240

Query: 241 VHDRDSEIYDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGLHGS 300
           VHDRDSE YDNLKNTILKCKSIRSLSGFLDVSPRCL SIYPICSNLTSLNLSYAPGLHGS
Sbjct: 241 VHDRDSETYDNLKNTILKCKSIRSLSGFLDVSPRCLPSIYPICSNLTSLNLSYAPGLHGS 300

Query: 301 ELIKVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV 360
           EL+KVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV
Sbjct: 301 ELVKVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV 360

Query: 361 AISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILNPTKPDPVTGNALDEGFG 420
           AISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCIL+PTK DPVT   LDEGFG
Sbjct: 361 AISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILDPTKADPVTNQPLDEGFG 420

Query: 421 AIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKKLRK 480
           AIVQACKGLRRLSLSGLL+DQVFYYIGEYAKHLEMLSLAFAG +DKGMIHVLNGCKKLRK
Sbjct: 421 AIVQACKGLRRLSLSGLLSDQVFYYIGEYAKHLEMLSLAFAGGTDKGMIHVLNGCKKLRK 480

Query: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCQITLGGCKTLAKKMPRLNVEIIDEDDQSE 540
           LEIMDSPFGDMALLQDVGKYETMRSLWMSSC+ITLGGCKTLAKKMPRLNVEII+E+D SE
Sbjct: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCEITLGGCKTLAKKMPRLNVEIINENDPSE 540

Query: 541 FCRDDGQKVGKMYLYRTLVGPRKDAPKFVWTL 573
           F RDDG++VGKMYLYRTLVGPRKDAPKFVWT+
Sbjct: 541 FGRDDGKRVGKMYLYRTLVGPRKDAPKFVWTV 572

BLAST of Lcy09g021070 vs. NCBI nr
Match: XP_022983583.1 (protein AUXIN SIGNALING F-BOX 2-like [Cucurbita maxima])

HSP 1 Score: 1119.8 bits (2895), Expect = 0.0e+00
Identity = 541/572 (94.58%), Postives = 559/572 (97.73%), Query Frame = 0

Query: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRTKVFVGNCYAITPERLIGRF 60
           MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWY+VERFSR KVFVGNCY+ITPERLIGRF
Sbjct: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYKVERFSRQKVFVGNCYSITPERLIGRF 60

Query: 61  PGVKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRIALEELRLKRMVVTDDSLELL 120
           P VKSLTLKGKPHFADFNLVPHDWGGYV+PWIQAFAKRRIALEELRLKRMVVTD+SLELL
Sbjct: 61  PCVKSLTLKGKPHFADFNLVPHDWGGYVHPWIQAFAKRRIALEELRLKRMVVTDESLELL 120

Query: 121 SRSFSNFKSLFLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPENCTS 180
           SRSF NFKSL LFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPE+CTS
Sbjct: 121 SRSFPNFKSLLLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPESCTS 180

Query: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHSILERAPQLVDLGTGSY 240
           LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETL +IL RAPQLVDLGTGSY
Sbjct: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLQNILVRAPQLVDLGTGSY 240

Query: 241 VHDRDSEIYDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGLHGS 300
           VHD+DSEIYDNLKNTILKCKSIRSLSGFLDVSP CLASIYPICSNLTSLNLSYAPGLHG 
Sbjct: 241 VHDQDSEIYDNLKNTILKCKSIRSLSGFLDVSPSCLASIYPICSNLTSLNLSYAPGLHGI 300

Query: 301 ELIKVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV 360
           EL+KV+RYC+KLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV
Sbjct: 301 ELMKVVRYCDKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV 360

Query: 361 AISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILNPTKPDPVTGNALDEGFG 420
           AISMGCPKLHSILYFCHQMTNAALV VAKNNPNFIRFRLCIL+PTKPDPVTG+ALDEGFG
Sbjct: 361 AISMGCPKLHSILYFCHQMTNAALVAVAKNNPNFIRFRLCILDPTKPDPVTGHALDEGFG 420

Query: 421 AIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKKLRK 480
           AIVQACKGLRRLSLSG LTD+VFYYIG+YAKHLEMLSLAFAGDSDKGMIH+LNGCKKLRK
Sbjct: 421 AIVQACKGLRRLSLSGHLTDRVFYYIGKYAKHLEMLSLAFAGDSDKGMIHILNGCKKLRK 480

Query: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCQITLGGCKTLAKKMPRLNVEIIDEDDQSE 540
           LEIMDSPFGDMALLQDVGKYETMRSLWMSSC+ITLGGCKTLAKKMPRLNVEII+E+D SE
Sbjct: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCEITLGGCKTLAKKMPRLNVEIINENDLSE 540

Query: 541 FCRDDGQKVGKMYLYRTLVGPRKDAPKFVWTL 573
             RDDGQKVGKMYLYRTLVGPRKDAPKFVWTL
Sbjct: 541 LSRDDGQKVGKMYLYRTLVGPRKDAPKFVWTL 572

BLAST of Lcy09g021070 vs. NCBI nr
Match: XP_022934783.1 (protein AUXIN SIGNALING F-BOX 2-like [Cucurbita moschata] >KAG6581127.1 Protein AUXIN SIGNALING F-BOX 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017861.1 Protein AUXIN SIGNALING F-BOX 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1119.4 bits (2894), Expect = 0.0e+00
Identity = 541/572 (94.58%), Postives = 558/572 (97.55%), Query Frame = 0

Query: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRTKVFVGNCYAITPERLIGRF 60
           MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSR KVFVGNCY+ITPERLIGRF
Sbjct: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRQKVFVGNCYSITPERLIGRF 60

Query: 61  PGVKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRIALEELRLKRMVVTDDSLELL 120
           P VKSLTLKGKPHFADFNLVPHDWGGYV+PWIQAFAKRRIALEELRLKRMVVTD+SLELL
Sbjct: 61  PCVKSLTLKGKPHFADFNLVPHDWGGYVHPWIQAFAKRRIALEELRLKRMVVTDESLELL 120

Query: 121 SRSFSNFKSLFLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPENCTS 180
           SRSF NFKSL LFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPE+CTS
Sbjct: 121 SRSFPNFKSLLLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPESCTS 180

Query: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHSILERAPQLVDLGTGSY 240
           LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETL +IL RAPQLVDLGTGSY
Sbjct: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLQNILVRAPQLVDLGTGSY 240

Query: 241 VHDRDSEIYDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGLHGS 300
           VHD+DSEIYDNLKNTILKCKSIRSLSGFLDVSP CLASIYPICSNLTSLNLSYAPGLHG 
Sbjct: 241 VHDQDSEIYDNLKNTILKCKSIRSLSGFLDVSPSCLASIYPICSNLTSLNLSYAPGLHGI 300

Query: 301 ELIKVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV 360
           EL+KV+RYC+KLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV
Sbjct: 301 ELMKVVRYCDKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV 360

Query: 361 AISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILNPTKPDPVTGNALDEGFG 420
           AISMGCPKLHSILYFCHQMTNAALV VAKNNPNFIRFRLCIL+PTKPDPVTG+ALDEGFG
Sbjct: 361 AISMGCPKLHSILYFCHQMTNAALVAVAKNNPNFIRFRLCILDPTKPDPVTGHALDEGFG 420

Query: 421 AIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKKLRK 480
           AIVQACKGLRRLSLSG LTD+VFYYIG+YAKHLEMLSLAFAGDSDKGMIH+LNGCKKLRK
Sbjct: 421 AIVQACKGLRRLSLSGHLTDRVFYYIGKYAKHLEMLSLAFAGDSDKGMIHILNGCKKLRK 480

Query: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCQITLGGCKTLAKKMPRLNVEIIDEDDQSE 540
           LEIMDSPFGDMALLQDVGKYETMRSLWMSSC+ITLGGCKTLAKKMPRLNVEII+E+D  E
Sbjct: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCEITLGGCKTLAKKMPRLNVEIINENDLLE 540

Query: 541 FCRDDGQKVGKMYLYRTLVGPRKDAPKFVWTL 573
            CRDD QKVGKMYLYRTLVGPRKDAPKFVWTL
Sbjct: 541 LCRDDEQKVGKMYLYRTLVGPRKDAPKFVWTL 572

BLAST of Lcy09g021070 vs. TAIR 10
Match: AT3G26810.1 (auxin signaling F-box 2 )

HSP 1 Score: 909.1 bits (2348), Expect = 1.8e-264
Identity = 437/575 (76.00%), Postives = 492/575 (85.57%), Query Frame = 0

Query: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRTKVFVGNCYAITPERLIGRF 60
           MNYFPDEV+EHVFD+VTSH+DRNA+SLVCK WY++ER+SR KVF+GNCYAI PERL+ RF
Sbjct: 1   MNYFPDEVIEHVFDFVTSHKDRNAISLVCKSWYKIERYSRQKVFIGNCYAINPERLLRRF 60

Query: 61  PGVKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRIALEELRLKRMVVTDDSLELL 120
           P +KSLTLKGKPHFADFNLVPH+WGG+V PWI+A A+ R+ LEELRLKRMVVTD+SLELL
Sbjct: 61  PCLKSLTLKGKPHFADFNLVPHEWGGFVLPWIEALARSRVGLEELRLKRMVVTDESLELL 120

Query: 121 SRSFSNFKSLFLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPENCTS 180
           SRSF NFKSL L SCEGFTT+GLA+IAANCR LR+LDLQENEIDDH   WLSCFP+ CT+
Sbjct: 121 SRSFVNFKSLVLVSCEGFTTDGLASIAANCRHLRDLDLQENEIDDHRGQWLSCFPDTCTT 180

Query: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHSILERAPQLVDLGTGSY 240
           LV+LNFACL GE NL ALERLVARSPNLKSL+LNRAVPL+ L  ++  APQ+VDLG GSY
Sbjct: 181 LVTLNFACLEGETNLVALERLVARSPNLKSLKLNRAVPLDALARLMACAPQIVDLGVGSY 240

Query: 241 VHDRDSEIYDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGLHGS 300
            +D DSE Y  L   I KC S+RSLSGFL+ +P CL++ +PIC NLTSLNLSYA  +HGS
Sbjct: 241 ENDPDSESYLKLMAVIKKCTSLRSLSGFLEAAPHCLSAFHPICHNLTSLNLSYAAEIHGS 300

Query: 301 ELIKVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEEGLV 360
            LIK+I++C+KLQRLWILD IGDKGLEVVASTCKELQELRVFPSDL G GN AVTEEGLV
Sbjct: 301 HLIKLIQHCKKLQRLWILDSIGDKGLEVVASTCKELQELRVFPSDLLGGGNTAVTEEGLV 360

Query: 361 AISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILNPTKPDPVTGNALDEGFG 420
           AIS GCPKLHSILYFC QMTNAALVTVAKN PNFIRFRLCIL P KPD VT   LDEGFG
Sbjct: 361 AISAGCPKLHSILYFCQQMTNAALVTVAKNCPNFIRFRLCILEPNKPDHVTSQPLDEGFG 420

Query: 421 AIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKKLRK 480
           AIV+ACK LRRLSLSGLLTDQVF YIG YA  LEMLS+AFAGD+DKGM++VLNGCKK++K
Sbjct: 421 AIVKACKSLRRLSLSGLLTDQVFLYIGMYANQLEMLSIAFAGDTDKGMLYVLNGCKKMKK 480

Query: 481 LEIMDSPFGDMALLQDVGKYETMRSLWMSSCQITLGGCKTLAKKMPRLNVEIIDEDDQS- 540
           LEI DSPFGD ALL DV KYETMRSLWMSSC++TL GCK LA+K P LNVEII+E+D + 
Sbjct: 481 LEIRDSPFGDTALLADVSKYETMRSLWMSSCEVTLSGCKRLAEKAPWLNVEIINENDNNR 540

Query: 541 --EFCRDDGQKVGKMYLYRTLVGPRKDAPKFVWTL 573
             E   +  QKV K+YLYRT+VG R DAP FVW L
Sbjct: 541 MEENGHEGRQKVDKLYLYRTVVGTRMDAPPFVWIL 575

BLAST of Lcy09g021070 vs. TAIR 10
Match: AT1G12820.1 (auxin signaling F-box 3 )

HSP 1 Score: 874.8 bits (2259), Expect = 3.8e-254
Identity = 417/574 (72.65%), Postives = 491/574 (85.54%), Query Frame = 0

Query: 1   MNYFPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRTKVFVGNCYAITPERLIGRF 60
           MNYFPDEV+EHVFD+V SH+DRN++SLVCK W+++ERFSR +VF+GNCYAI PERLI RF
Sbjct: 1   MNYFPDEVIEHVFDFVASHKDRNSISLVCKSWHKIERFSRKEVFIGNCYAINPERLIRRF 60

Query: 61  PGVKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRIALEELRLKRMVVTDDSLELL 120
           P +KSLTLKGKPHFADFNLVPH+WGG+V+PWI+A A+ R+ LEELRLKRMVVTD+SL+LL
Sbjct: 61  PCLKSLTLKGKPHFADFNLVPHEWGGFVHPWIEALARSRVGLEELRLKRMVVTDESLDLL 120

Query: 121 SRSFSNFKSLFLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPENCTS 180
           SRSF+NFKSL L SCEGFTT+GLA+IAANCR LRELDLQENEIDDH   WL+CFP++CT+
Sbjct: 121 SRSFANFKSLVLVSCEGFTTDGLASIAANCRHLRELDLQENEIDDHRGQWLNCFPDSCTT 180

Query: 181 LVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHSILERAPQLVDLGTGSY 240
           L+SLNFACL+GE N+ ALERLVARSPNLKSL+LNRAVPL+ L  ++  APQLVDLG GSY
Sbjct: 181 LMSLNFACLKGETNVAALERLVARSPNLKSLKLNRAVPLDALARLMSCAPQLVDLGVGSY 240

Query: 241 VHDRDSEIYDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGLHGS 300
            ++ D E +  L   I K  S+RSLSGFL+V+P CL + YPIC NL SLNLSYA  + G+
Sbjct: 241 ENEPDPESFAKLMTAIKKYTSLRSLSGFLEVAPLCLPAFYPICQNLISLNLSYAAEIQGN 300

Query: 301 ELIKVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGA--GNVAVTEEG 360
            LIK+I+ C++LQRLWILD IGDKGL VVA+TCKELQELRVFPSD+ G    N +VTE G
Sbjct: 301 HLIKLIQLCKRLQRLWILDSIGDKGLAVVAATCKELQELRVFPSDVHGEEDNNASVTEVG 360

Query: 361 LVAISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILNPTKPDPVTGNALDEG 420
           LVAIS GCPKLHSILYFC QMTNAAL+ VAKN PNFIRFRLCIL P KPD +T  +LDEG
Sbjct: 361 LVAISAGCPKLHSILYFCKQMTNAALIAVAKNCPNFIRFRLCILEPHKPDHITFQSLDEG 420

Query: 421 FGAIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKKL 480
           FGAIVQACKGLRRLS+SGLLTDQVF YIG YA+ LEMLS+AFAGD+DKGM++VLNGCKK+
Sbjct: 421 FGAIVQACKGLRRLSVSGLLTDQVFLYIGMYAEQLEMLSIAFAGDTDKGMLYVLNGCKKM 480

Query: 481 RKLEIMDSPFGDMALLQDVGKYETMRSLWMSSCQITLGGCKTLAKKMPRLNVEIIDEDDQ 540
           RKLEI DSPFG+ ALL DVG+YETMRSLWMSSC++TLGGCK LA+  PRLNVEII+E++ 
Sbjct: 481 RKLEIRDSPFGNAALLADVGRYETMRSLWMSSCEVTLGGCKRLAQNSPRLNVEIINENEN 540

Query: 541 S---EFCRDDGQKVGKMYLYRTLVGPRKDAPKFV 570
           +   +   D+ +KV K+YLYRT+VG RKDAP +V
Sbjct: 541 NGMEQNEEDEREKVDKLYLYRTVVGTRKDAPPYV 574

BLAST of Lcy09g021070 vs. TAIR 10
Match: AT3G62980.1 (F-box/RNI-like superfamily protein )

HSP 1 Score: 734.2 bits (1894), Expect = 8.1e-212
Identity = 354/570 (62.11%), Postives = 437/570 (76.67%), Query Frame = 0

Query: 4   FPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRTKVFVGNCYAITPERLIGRFPGV 63
           FP+EVLEHVF ++   +DRN+VSLVCK WY +ER+ R KVF+GNCYA++P  +I RFP V
Sbjct: 9   FPEEVLEHVFSFIQLDKDRNSVSLVCKSWYEIERWCRRKVFIGNCYAVSPATVIRRFPKV 68

Query: 64  KSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRIALEELRLKRMVVTDDSLELLSRS 123
           +S+ LKGKPHFADFNLVP  WGGYVYPWI+A +     LEE+RLKRMVVTDD LEL+++S
Sbjct: 69  RSVELKGKPHFADFNLVPDGWGGYVYPWIEAMSSSYTWLEEIRLKRMVVTDDCLELIAKS 128

Query: 124 FSNFKSLFLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPENCTSLVS 183
           F NFK L L SCEGF+T+GLAAIAA CR L+ELDL+E+++DD S +WLS FP+  TSLVS
Sbjct: 129 FKNFKVLVLSSCEGFSTDGLAAIAATCRNLKELDLRESDVDDVSGHWLSHFPDTYTSLVS 188

Query: 184 LNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHSILERAPQLVDLGTGSYVHD 243
           LN +CL  EV+  ALERLV R PNLKSL+LNRAVPLE L ++L+RAPQL +LGTG Y  +
Sbjct: 189 LNISCLASEVSFSALERLVTRCPNLKSLKLNRAVPLEKLATLLQRAPQLEELGTGGYTAE 248

Query: 244 RDSEIYDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGLHGSELI 303
              ++Y  L   +  CK +R LSGF D  P  L ++Y +CS LT+LNLSYA  +   +L+
Sbjct: 249 VRPDVYSGLSVALSGCKELRCLSGFWDAVPAYLPAVYSVCSRLTTLNLSYAT-VQSYDLV 308

Query: 304 KVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSD-LSGAGNVAVTEEGLVAI 363
           K++  C KLQRLW+LD I D GLEV+ASTCK+L+ELRVFPS+      NVA+TE+GLV++
Sbjct: 309 KLLCQCPKLQRLWVLDYIEDAGLEVLASTCKDLRELRVFPSEPFVMEPNVALTEQGLVSV 368

Query: 364 SMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILNPTKPDPVTGNALDEGFGAI 423
           SMGCPKL S+LYFC QMTNAAL+T+A+N PN  RFRLCI+ P  PD +T   LD GFGAI
Sbjct: 369 SMGCPKLESVLYFCRQMTNAALITIARNRPNMTRFRLCIIEPKAPDYLTLEPLDIGFGAI 428

Query: 424 VQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKKLRKLE 483
           V+ CK LRRLSLSGLLTD+VF YIG YAK +EMLS+AFAGDSD GM HVL+GC  LRKLE
Sbjct: 429 VEHCKDLRRLSLSGLLTDKVFEYIGTYAKKMEMLSVAFAGDSDLGMHHVLSGCDSLRKLE 488

Query: 484 IMDSPFGDMALLQDVGKYETMRSLWMSSCQITLGGCKTLAKKMPRLNVEIIDEDDQSEFC 543
           I D PFGD ALL +  K ETMRSLWMSSC ++ G CK L +KMP+LNVE+IDE    +  
Sbjct: 489 IRDCPFGDKALLANASKLETMRSLWMSSCSVSFGACKLLGQKMPKLNVEVIDERGAPD-S 548

Query: 544 RDDGQKVGKMYLYRTLVGPRKDAPKFVWTL 573
           R +   V ++++YRT+ GPR D P FVW +
Sbjct: 549 RPESCPVERVFIYRTVAGPRFDMPGFVWNM 576

BLAST of Lcy09g021070 vs. TAIR 10
Match: AT4G03190.1 (GRR1-like protein 1 )

HSP 1 Score: 668.3 bits (1723), Expect = 5.5e-192
Identity = 322/570 (56.49%), Postives = 418/570 (73.33%), Query Frame = 0

Query: 4   FPDEVLEHVFDYVTSHRDRNAVSLVCKLWYRVERFSRTKVFVGNCYAITPERLIGRFPGV 63
           FP +VLEH+  ++ S+ DRN+VSLVCK W+  ER +R +VFVGNCYA++P  +  RFP +
Sbjct: 5   FPPKVLEHILSFIDSNEDRNSVSLVCKSWFETERKTRKRVFVGNCYAVSPAAVTRRFPEM 64

Query: 64  KSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRIALEELRLKRMVVTDDSLELLSRS 123
           +SLTLKGKPHFAD+NLVP  WGGY +PWI+A A +  +LEE+R+KRMVVTD+ LE ++ S
Sbjct: 65  RSLTLKGKPHFADYNLVPDGWGGYAWPWIEAMAAKSSSLEEIRMKRMVVTDECLEKIAAS 124

Query: 124 FSNFKSLFLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPENCTSLVS 183
           F +FK L L SCEGF+T+G+AAIAA CR LR L+L+E  ++D    WLS FPE+ TSLVS
Sbjct: 125 FKDFKVLVLTSCEGFSTDGIAAIAATCRNLRVLELRECIVEDLGGDWLSYFPESSTSLVS 184

Query: 184 LNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHSILERAPQLVDLGTGSYVHD 243
           L+F+CL  EV +  LERLV+RSPNLKSL+LN AV L+ L S+L  APQL +LGTGS+   
Sbjct: 185 LDFSCLDSEVKISDLERLVSRSPNLKSLKLNPAVTLDGLVSLLRCAPQLTELGTGSFAAQ 244

Query: 244 RDSEIYDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGLHGSELI 303
              E +  L      CK ++SLSG  DV P  L ++Y +C  LTSLNLSYA  +   +L+
Sbjct: 245 LKPEAFSKLSEAFSNCKQLQSLSGLWDVLPEYLPALYSVCPGLTSLNLSYAT-VRMPDLV 304

Query: 304 KVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLS-GAGNVAVTEEGLVAI 363
           +++R C KLQ+LW++D I DKGLE VAS CKEL+ELRVFPS+    A N+ +TE+GLV +
Sbjct: 305 ELLRRCSKLQKLWVMDLIEDKGLEAVASYCKELRELRVFPSEPDLDATNIPLTEQGLVFV 364

Query: 364 SMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILNPTKPDPVTGNALDEGFGAI 423
           S GC KL S+LYFC Q TNAAL T+A+  PN   FRLC++ P  PD  T   LD+GF AI
Sbjct: 365 SKGCRKLESVLYFCVQFTNAALFTIARKRPNLKCFRLCVIEPFAPDYKTNEPLDKGFKAI 424

Query: 424 VQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKKLRKLE 483
            + C+ LRRLS+SGLL+D+ F YIG++AK + MLS+AFAGDSD  + H+L+GC+ L+KLE
Sbjct: 425 AEGCRDLRRLSVSGLLSDKAFKYIGKHAKKVRMLSIAFAGDSDLMLHHLLSGCESLKKLE 484

Query: 484 IMDSPFGDMALLQDVGKYETMRSLWMSSCQITLGGCKTLAKKMPRLNVEIIDEDDQSEFC 543
           I D PFGD ALL+   K ETMRSLWMSSC ++ G CK L++KMPRLNVE+IDE       
Sbjct: 485 IRDCPFGDTALLEHAAKLETMRSLWMSSCFVSFGACKLLSQKMPRLNVEVIDEHPPES-- 544

Query: 544 RDDGQKVGKMYLYRTLVGPRKDAPKFVWTL 573
           R +   V ++Y+YRT+ GPR D P+FVWT+
Sbjct: 545 RPESSPVERIYIYRTVAGPRMDTPEFVWTI 571

BLAST of Lcy09g021070 vs. TAIR 10
Match: AT5G49980.1 (auxin F-box protein 5 )

HSP 1 Score: 598.2 bits (1541), Expect = 6.9e-171
Identity = 305/575 (53.04%), Postives = 385/575 (66.96%), Query Frame = 0

Query: 4   FPDEVLEHVFD----YVTSHRDRNAVSLVCKLWYRVERFSRTKVFVGNCYAITPERLIGR 63
           FPD VLE+V +    ++ S  DRNA SLVCK W+RVE  +R++VF+GNCYA++P RL  R
Sbjct: 50  FPDHVLENVLENVLQFLDSRCDRNAASLVCKSWWRVEALTRSEVFIGNCYALSPARLTQR 109

Query: 64  FPGVKSLTLKGKPHFADFNLVPHDWGGYVYPWIQAFAKRRIALEELRLKRMVVTDDSLEL 123
           F  V+SL LKGKP FADFNL+P DWG    PW+   A+    LE++ LKRM VTDD L L
Sbjct: 110 FKRVRSLVLKGKPRFADFNLMPPDWGANFAPWVSTMAQAYPCLEKVDLKRMFVTDDDLAL 169

Query: 124 LSRSFSNFKSLFLFSCEGFTTNGLAAIAANCRFLRELDLQENEIDDHSNYWLSCFPENCT 183
           L+ SF  FK L L  CEGF T+G++ +A  CR L+ LDL E+E+ D    W+SCFPE+ T
Sbjct: 170 LADSFPGFKELILVCCEGFGTSGISIVANKCRKLKVLDLIESEVTDDEVDWISCFPEDVT 229

Query: 184 SLVSLNFACLRGEVNLGALERLVARSPNLKSLRLNRAVPLETLHSILERAPQLVDLGTGS 243
            L SL F C+   +N  ALE LVARSP LK LRLNR V L  LH +L  APQL  LGTGS
Sbjct: 230 CLESLAFDCVEAPINFKALEGLVARSPFLKKLRLNRFVSLVELHRLLLGAPQLTSLGTGS 289

Query: 244 YVHDRD--SEIYDNLKNTILKCKSIRSLSGFLDVSPRCLASIYPICSNLTSLNLSYAPGL 303
           + HD +  SE   +       CKS+  LSGF ++ P  L +I+P+C+NLTSLN SYA  +
Sbjct: 290 FSHDEEPQSEQEPDYAAAFRACKSVVCLSGFRELMPEYLPAIFPVCANLTSLNFSYA-NI 349

Query: 304 HGSELIKVIRYCEKLQRLWILDGIGDKGLEVVASTCKELQELRVFPSDLSGAGNVAVTEE 363
                  +I  C KLQ  W LD I D+GL+ VA+TCKEL+ELR+FP D        V+E 
Sbjct: 350 SPDMFKPIILNCHKLQVFWALDSICDEGLQAVAATCKELRELRIFPFDPREDSEGPVSEL 409

Query: 364 GLVAISMGCPKLHSILYFCHQMTNAALVTVAKNNPNFIRFRLCILNPTKPDPVTGNALDE 423
           GL AIS GC KL SILYFC +MTNAA++ +++N P    FRLCI+   +PD VTG  +DE
Sbjct: 410 GLQAISEGCRKLESILYFCQRMTNAAVIAMSENCPELTVFRLCIMGRHRPDHVTGKPMDE 469

Query: 424 GFGAIVQACKGLRRLSLSGLLTDQVFYYIGEYAKHLEMLSLAFAGDSDKGMIHVLNGCKK 483
           GFGAIV+ CK L RL++SGLLTDQ F Y+GEY K +  LS+AFAGDSD  + HVL GC +
Sbjct: 470 GFGAIVKNCKKLTRLAVSGLLTDQAFRYMGEYGKLVRTLSVAFAGDSDMALRHVLEGCPR 529

Query: 484 LRKLEIMDSPFGDMALLQDVGKYETMRSLWMSSCQITLGGCKTLAKKMPRLNVEIIDEDD 543
           L+KLEI DSPFGD+AL   + +Y  MR +WMS+C ++ G CK +A+ MP L VE+I  DD
Sbjct: 530 LQKLEIRDSPFGDVALRSGMHRYYNMRFVWMSACSLSKGCCKDIARAMPNLVVEVIGSDD 589

Query: 544 QSEFCRDDGQKVGKMYLYRTLVGPRKDAPKFVWTL 573
                 D+   V  +Y+YR+L GPR DAPKFV  L
Sbjct: 590 DD----DNRDYVETLYMYRSLDGPRNDAPKFVTIL 619

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LW292.6e-26376.00Protein AUXIN SIGNALING F-BOX 2 OS=Arabidopsis thaliana OX=3702 GN=AFB2 PE=1 SV=... [more]
Q9LPW75.4e-25372.65Protein AUXIN SIGNALING F-BOX 3 OS=Arabidopsis thaliana OX=3702 GN=AFB3 PE=1 SV=... [more]
Q7XVM84.8e-23367.83Transport inhibitor response 1-like protein Os04g0395600 OS=Oryza sativa subsp. ... [more]
Q570C01.1e-21062.11Protein TRANSPORT INHIBITOR RESPONSE 1 OS=Arabidopsis thaliana OX=3702 GN=TIR1 P... [more]
Q2R3K55.1e-19558.93Transport inhibitor response 1-like protein Os11g0515500 OS=Oryza sativa subsp. ... [more]
Match NameE-valueIdentityDescription
A0A6J1DQR90.0e+0094.76protein AUXIN SIGNALING F-BOX 2-like OS=Momordica charantia OX=3673 GN=LOC111023... [more]
A0A6J1J8520.0e+0094.58protein AUXIN SIGNALING F-BOX 2-like OS=Cucurbita maxima OX=3661 GN=LOC111482144... [more]
A0A6J1F8M30.0e+0094.58protein AUXIN SIGNALING F-BOX 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114418... [more]
A0A5D3DU600.0e+0091.99Protein AUXIN SIGNALING F-BOX 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BMP80.0e+0091.82protein AUXIN SIGNALING F-BOX 2-like OS=Cucumis melo OX=3656 GN=LOC103491723 PE=... [more]
Match NameE-valueIdentityDescription
XP_038893828.10.0e+0096.85protein AUXIN SIGNALING F-BOX 2-like [Benincasa hispida][more]
XP_023527075.10.0e+0094.76protein AUXIN SIGNALING F-BOX 2-like [Cucurbita pepo subsp. pepo][more]
XP_022156493.10.0e+0094.76protein AUXIN SIGNALING F-BOX 2-like [Momordica charantia][more]
XP_022983583.10.0e+0094.58protein AUXIN SIGNALING F-BOX 2-like [Cucurbita maxima][more]
XP_022934783.10.0e+0094.58protein AUXIN SIGNALING F-BOX 2-like [Cucurbita moschata] >KAG6581127.1 Protein ... [more]
Match NameE-valueIdentityDescription
AT3G26810.11.8e-26476.00auxin signaling F-box 2 [more]
AT1G12820.13.8e-25472.65auxin signaling F-box 3 [more]
AT3G62980.18.1e-21262.11F-box/RNI-like superfamily protein [more]
AT4G03190.15.5e-19256.49GRR1-like protein 1 [more]
AT5G49980.16.9e-17153.04auxin F-box protein 5 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006553Leucine-rich repeat, cysteine-containing subtypeSMARTSM00367LRR_CC_2coord: 426..449
e-value: 110.0
score: 6.4
coord: 124..149
e-value: 23.0
score: 11.8
coord: 99..123
e-value: 420.0
score: 1.9
coord: 283..308
e-value: 120.0
score: 6.2
coord: 366..390
e-value: 11.0
score: 14.3
IPR001810F-box domainSMARTSM00256fbox_2coord: 4..45
e-value: 4.2E-4
score: 29.6
NoneNo IPR availableGENE3D1.20.1280.50coord: 1..40
e-value: 1.2E-18
score: 68.3
NoneNo IPR availablePANTHERPTHR16134:SF54PROTEIN AUXIN SIGNALING F-BOX 3coord: 2..569
NoneNo IPR availablePANTHERPTHR16134F-BOX/TPR REPEAT PROTEIN POF3coord: 2..569
NoneNo IPR availableSUPERFAMILY52047RNI-likecoord: 16..436
NoneNo IPR availableSUPERFAMILY52047RNI-likecoord: 419..527
IPR041101Transport inhibitor response 1 domainPFAMPF18791Transp_inhibitcoord: 61..107
e-value: 6.8E-24
score: 83.1
IPR041567COI1, F-boxPFAMPF18511F-box_5coord: 2..41
e-value: 6.6E-21
score: 73.6
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 41..572
e-value: 4.3E-175
score: 585.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g021070.1Lcy09g021070.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009734 auxin-activated signaling pathway
biological_process GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
cellular_component GO:0019005 SCF ubiquitin ligase complex
molecular_function GO:0005515 protein binding