Lcy09g019640 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g019640
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionPectin lyase-like superfamily protein
LocationChr09: 44446820 .. 44450344 (-)
RNA-Seq ExpressionLcy09g019640
SyntenyLcy09g019640
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATCAATGTCCTTAATGGTGTGTCTATAGTTTATTTTCTCTGCTGGCTTGTTTTTGCCACAAATCTTAGCTTGGGTTTTTAAGGAAGAAGGCGGCCGCCGAATTCAATTAATCCAAATGAGAGAGAGAGGGAATAAAAAATAGATCAGCTTGATAGAAAAAGAATTATATAAGCCATACAGTAAACCAGAGGAGGAAGAAGAAGAAAAGGGAGTTTTGTCACCATCCAAGTTGTCTTTTGAACTGTGGAGCTCATGTGTGTATAGTATATCTAACATAGAACTAGTTTTCGACCTATAAATACAAGCCTATAATCTTCTCTGTTTGTCAATCATACTTACAGAATTCTCTCTCTCTATCTCTCTTTCTGTCTTTCTCTACCTATTTGATATGGAGATGGGTGGGAAGAACTTGAAATGTCTTGCAACTATTGCTTTCATTTGTGTTTTTGTTTGGTCTTTCAGTTTGCAAAATTGCCATGCTAGAGAAGGCAGGCATTGGGGGGCACATAGAAAAACTGGTGGCTACCGCCACCGCCACGCTCATCGTCACCGTGGAGGTAGCGGTTCGTCAAGGTTGATGGGTAGTGGTGATGTTGTGGGAGCCTCTCTAGCAAGTAAAGGTGGTGCTAGTAAAGGTGGAGATGGTTCTGGCAATTTTAATGTGTTGGACTTTGGAGCTAAAGGTGATGGAAGGACAGATGACACTAAGGTGAATTTGGGTTTACTTATCATTTTCATTATATTGAAATTGCACAAACATTTATACTTTACAGAATTGAGAACTCACAGAAGTGCTTGACAATGTATGCATACCAGGCATTTCAGGCAGCTTGGGAATCAGCTTGCAATGCAGAGGGATCAACTGTGGAGGTTCCCTCAGGTTCCGAATTCCTTGTTGGCCCCATTTCGTTCTCTGGTCCTAATTGCCAGCCAAACATTGTGTTTCAGGTCAATATCAAAACACTTAAGTCTGTTTTGCAAGTTGATTTCAGAACAGTCTTTAAAGTCCACAGTAACATAATGCTTTAAAAGGATTCCAATCAACCTTATAAAGAACTTTCCAGAAAGCTCCTTTATTATGCAGATAAAAAATTTCTTGATGACATATTTTAAAGTACATCTCCTACATTGACATGCAGTTGGATGGCAAAATTATCGCTCCTACAAGCCCTAGTGCTTGGGGTTCAGGTCTCATACAATGGATTGAATTCACTAAACTTAAAGGGATTACAGTGAAAGGTACGGGCACTATTGATGGACAAGGCTCAGCTTGGTGGAACGACTCACCCACATATGATCCTACCGACGAAAATTTGGTAATTTTGTAAATCGACTCGATAAGAATCAAAAGTATTTCATATTTCAAGTCAAACAGCTACCTAATCACAGCTTAATTCTCATGCAACAGGACAAGGAAAGCAGCAGCGGAAAACTTCCAAGCACCAAACCAACAGTACTGCCTTGGCTTAAACTTGACAATAATAAACAAATGATCAAAGAGGGTATTCTGTTTGACTCCGATATTCTTGTGCAGGCACTTAGGTTCTATGGAAGTGATGGAGTGACAGTCACTGGAATAACCATCCAAAACAGTCAACAGGCTCACCTGAAATTTGATAGTTGCACATCAGTTCAGGTTTCTTCATTAACCGTGTCATCGCCGGGTGACAGCCCAAACACAGATGGAATTCACTTGCAGAACTCTCAGAATGTGATCATTTCAAACAGCAATTTGGCTTGTGGTAATTCCCAATTTTGAGTAGTAACTGCAAAGATGGCTTTCGTTAGCATGCAAGTTTAACCTTAAAAAGAATTCCAACTTTACATAAGCAGATAAATATAAGGTAACCCAATTGGTTAAAAGTCAGAAAGTTGATCTTCAACCAGCAAGTCATTTAAAGGTGGCTTTAGATTTGTTTTTTGCATAACAGTTCTGAGAACTTTTTTTATTGATCTGGTTTTAGAAACTTGAATAAGGTCCTAAAACATGAGGTTACTGTGTACTTGACGTCCTTATTCGCCAGCCTGTACTTTTGAAGGAAGATCAACTTCAGTTGTTTTGAAAAAGCAACTAATCCATGAAAACGAATTTGAATAAACATTTAACCCAGCATGTATTCACTAAAAAAACAAACAATGGTCTTTAAACTCAACTGCCAAGTAGAACCTTCTTCCCTAAAAAACTAATCTGATGTGACCACAAACTTCACTCTTGTGACAGAGGATAAAATTCTTCCAGCTATGGAATTTAAATAATCAGTAGCATTATGAAACTTATATAAATAAAAAAATCGAACTACAGGAGATGATTGTATATCCATACAAACTGGAAGCTCGGCCATATATATACACGACGTCAACTGTGGACCAGGCCATGGAATTAGCATTGGAGGACTAGGACGTGATCAAACAAAAGCATGTGTTTCCAATGTCACAGTCCGTGACACCAAACTGCAGAATACAATGACAGGAGTCAGAATAAAAACATGGCAGGTATGTAAAACCTCTCTCCTCTAGTTTTGACTCCAACATGGTAATAAATGTATTCAGTAAAAGCAGGAGGAAAAAAAGCATCAATAAGCCCATCTTTGTTCAAACATACTTTTCCCAGTTGTATAGTTTGCCAAAAACTCCCTTTCTCAAACCAACCATAATTTCTAATTCAACAGCTAACAGAGGCCATTTAATTTTTCCCATTGCAGGGTGGTTCAGGCTCAGTACAGGGAATACTGTTCTCAAACATTCAAGTTTCAGACGTTCGAACTCCAATTATGATAGACCAATACTACTGCGATGGGGGTAGATGCCATAATGGATCTTCTGCAGTAGCAATTTCAGGAGTAAACTATGTAAACATTAGAGGAACATACACTTCAACGCCGGTACATTTAGCCTGCAGTGACAGCCTTCCCTGCACTGGTGTGACATTAGACACCATTCAGCTTGAGGGGAGTGGTAACTCAAATGAACCATTCTGTTGGAAGGCATATGGTGAACTGAAAACCTCCACTGTCCCTCCCATTGACTGCTTGCAGTCTGGCAACCCATCAAAAGCTCAGGCTCGCGATTCTTGCTAAGCTCAGCAGCTAAATATTTATTAGATTATAGATAGATATTTGCAGTCACTAATGGAGAGTTAGGAAAAATTTTACATGGTAGACTGCTTAGAAATAGAAGTGTTATATGCTAAAAAAAGGGTGCTATAGATATAGATATATGCAGTCACTGATGGAGAGTTAGGAGAAAATTTACATGGTAGACTGCTTAGAAATAGATGTGTTATATGCTTAGAAAAGTGTCCTATATATATGATATTGTTGCTACATCAATGGCCCTTACTCAGAATTCATCATTCATGCATAGTAAGATTAATTTATACTTCTATAAACCTCCCGAGTCACATCAAATCCAACTTATGAGAGACTATTGAATTACACAACGTTAAGATCAAGCTTTTAAGCTCAAAATTTCACCCTCCTCGCGAAATGATTAACACGTAAAGCACCTAATTACCAGATTATAAT

mRNA sequence

TATCAATGTCCTTAATGGTGTGTCTATAGTTTATTTTCTCTGCTGGCTTGTTTTTGCCACAAATCTTAGCTTGGGTTTTTAAGGAAGAAGGCGGCCGCCGAATTCAATTAATCCAAATGAGAGAGAGAGGGAATAAAAAATAGATCAGCTTGATAGAAAAAGAATTATATAAGCCATACAGTAAACCAGAGGAGGAAGAAGAAGAAAAGGGAGTTTTGTCACCATCCAAGTTGTCTTTTGAACTGTGGAGCTCATGTGTGTATAGTATATCTAACATAGAACTAGTTTTCGACCTATAAATACAAGCCTATAATCTTCTCTGTTTGTCAATCATACTTACAGAATTCTCTCTCTCTATCTCTCTTTCTGTCTTTCTCTACCTATTTGATATGGAGATGGGTGGGAAGAACTTGAAATGTCTTGCAACTATTGCTTTCATTTGTGTTTTTGTTTGGTCTTTCAGTTTGCAAAATTGCCATGCTAGAGAAGGCAGGCATTGGGGGGCACATAGAAAAACTGGTGGCTACCGCCACCGCCACGCTCATCGTCACCGTGGAGGTAGCGGTTCGTCAAGGTTGATGGGTAGTGGTGATGTTGTGGGAGCCTCTCTAGCAAGTAAAGGTGGTGCTAGTAAAGGTGGAGATGGTTCTGGCAATTTTAATGTGTTGGACTTTGGAGCTAAAGGTGATGGAAGGACAGATGACACTAAGGCATTTCAGGCAGCTTGGGAATCAGCTTGCAATGCAGAGGGATCAACTGTGGAGGTTCCCTCAGGTTCCGAATTCCTTGTTGGCCCCATTTCGTTCTCTGGTCCTAATTGCCAGCCAAACATTGTGTTTCAGTTGGATGGCAAAATTATCGCTCCTACAAGCCCTAGTGCTTGGGGTTCAGGTCTCATACAATGGATTGAATTCACTAAACTTAAAGGGATTACAGTGAAAGGTACGGGCACTATTGATGGACAAGGCTCAGCTTGGTGGAACGACTCACCCACATATGATCCTACCGACGAAAATTTGGACAAGGAAAGCAGCAGCGGAAAACTTCCAAGCACCAAACCAACAGCACTTAGGTTCTATGGAAGTGATGGAGTGACAGTCACTGGAATAACCATCCAAAACAGTCAACAGGCTCACCTGAAATTTGATAGTTGCACATCAGTTCAGGTTTCTTCATTAACCGTGTCATCGCCGGGTGACAGCCCAAACACAGATGGAATTCACTTGCAGAACTCTCAGAATGTGATCATTTCAAACAGCAATTTGGCTTGTGGAGATGATTGTATATCCATACAAACTGGAAGCTCGGCCATATATATACACGACGTCAACTGTGGACCAGGCCATGGAATTAGCATTGGAGGACTAGGACGTGATCAAACAAAAGCATGTGTTTCCAATGTCACAGTCCGTGACACCAAACTGCAGAATACAATGACAGGAGTCAGAATAAAAACATGGCAGGGTGGTTCAGGCTCAGTACAGGGAATACTGTTCTCAAACATTCAAGTTTCAGACGTTCGAACTCCAATTATGATAGACCAATACTACTGCGATGGGGGTAGATGCCATAATGGATCTTCTGCAGTAGCAATTTCAGGAGTAAACTATGTAAACATTAGAGGAACATACACTTCAACGCCGGTACATTTAGCCTGCAGTGACAGCCTTCCCTGCACTGGTGTGACATTAGACACCATTCAGCTTGAGGGGAGTGGTAACTCAAATGAACCATTCTGTTGGAAGGCATATGGTGAACTGAAAACCTCCACTGTCCCTCCCATTGACTGCTTGCAGTCTGGCAACCCATCAAAAGCTCAGGCTCGCGATTCTTGCTAAGCTCAGCAGCTAAATATTTATTAGATTATAGATAGATATTTGCAGTCACTAATGGAGAGTTAGGAAAAATTTTACATGGTAGACTGCTTAGAAATAGAAGTGTTATATGCTAAAAAAAGGGTGCTATAGATATAGATATATGCAGTCACTGATGGAGAGTTAGGAGAAAATTTACATGGTAGACTGCTTAGAAATAGATGTGTTATATGCTTAGAAAAGTGTCCTATATATATGATATTGTTGCTACATCAATGGCCCTTACTCAGAATTCATCATTCATGCATAGTAAGATTAATTTATACTTCTATAAACCTCCCGAGTCACATCAAATCCAACTTATGAGAGACTATTGAATTACACAACGTTAAGATCAAGCTTTTAAGCTCAAAATTTCACCCTCCTCGCGAAATGATTAACACGTAAAGCACCTAATTACCAGATTATAAT

Coding sequence (CDS)

ATGGAGATGGGTGGGAAGAACTTGAAATGTCTTGCAACTATTGCTTTCATTTGTGTTTTTGTTTGGTCTTTCAGTTTGCAAAATTGCCATGCTAGAGAAGGCAGGCATTGGGGGGCACATAGAAAAACTGGTGGCTACCGCCACCGCCACGCTCATCGTCACCGTGGAGGTAGCGGTTCGTCAAGGTTGATGGGTAGTGGTGATGTTGTGGGAGCCTCTCTAGCAAGTAAAGGTGGTGCTAGTAAAGGTGGAGATGGTTCTGGCAATTTTAATGTGTTGGACTTTGGAGCTAAAGGTGATGGAAGGACAGATGACACTAAGGCATTTCAGGCAGCTTGGGAATCAGCTTGCAATGCAGAGGGATCAACTGTGGAGGTTCCCTCAGGTTCCGAATTCCTTGTTGGCCCCATTTCGTTCTCTGGTCCTAATTGCCAGCCAAACATTGTGTTTCAGTTGGATGGCAAAATTATCGCTCCTACAAGCCCTAGTGCTTGGGGTTCAGGTCTCATACAATGGATTGAATTCACTAAACTTAAAGGGATTACAGTGAAAGGTACGGGCACTATTGATGGACAAGGCTCAGCTTGGTGGAACGACTCACCCACATATGATCCTACCGACGAAAATTTGGACAAGGAAAGCAGCAGCGGAAAACTTCCAAGCACCAAACCAACAGCACTTAGGTTCTATGGAAGTGATGGAGTGACAGTCACTGGAATAACCATCCAAAACAGTCAACAGGCTCACCTGAAATTTGATAGTTGCACATCAGTTCAGGTTTCTTCATTAACCGTGTCATCGCCGGGTGACAGCCCAAACACAGATGGAATTCACTTGCAGAACTCTCAGAATGTGATCATTTCAAACAGCAATTTGGCTTGTGGAGATGATTGTATATCCATACAAACTGGAAGCTCGGCCATATATATACACGACGTCAACTGTGGACCAGGCCATGGAATTAGCATTGGAGGACTAGGACGTGATCAAACAAAAGCATGTGTTTCCAATGTCACAGTCCGTGACACCAAACTGCAGAATACAATGACAGGAGTCAGAATAAAAACATGGCAGGGTGGTTCAGGCTCAGTACAGGGAATACTGTTCTCAAACATTCAAGTTTCAGACGTTCGAACTCCAATTATGATAGACCAATACTACTGCGATGGGGGTAGATGCCATAATGGATCTTCTGCAGTAGCAATTTCAGGAGTAAACTATGTAAACATTAGAGGAACATACACTTCAACGCCGGTACATTTAGCCTGCAGTGACAGCCTTCCCTGCACTGGTGTGACATTAGACACCATTCAGCTTGAGGGGAGTGGTAACTCAAATGAACCATTCTGTTGGAAGGCATATGGTGAACTGAAAACCTCCACTGTCCCTCCCATTGACTGCTTGCAGTCTGGCAACCCATCAAAAGCTCAGGCTCGCGATTCTTGCTAA

Protein sequence

MEMGGKNLKCLATIAFICVFVWSFSLQNCHAREGRHWGAHRKTGGYRHRHAHRHRGGSGSSRLMGSGDVVGASLASKGGASKGGDGSGNFNVLDFGAKGDGRTDDTKAFQAAWESACNAEGSTVEVPSGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSPSAWGSGLIQWIEFTKLKGITVKGTGTIDGQGSAWWNDSPTYDPTDENLDKESSSGKLPSTKPTALRFYGSDGVTVTGITIQNSQQAHLKFDSCTSVQVSSLTVSSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSAIYIHDVNCGPGHGISIGGLGRDQTKACVSNVTVRDTKLQNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVRTPIMIDQYYCDGGRCHNGSSAVAISGVNYVNIRGTYTSTPVHLACSDSLPCTGVTLDTIQLEGSGNSNEPFCWKAYGELKTSTVPPIDCLQSGNPSKAQARDSC
Homology
BLAST of Lcy09g019640 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 5.1e-103
Identity = 189/398 (47.49%), Postives = 253/398 (63.57%), Query Frame = 0

Query: 90  FNVLDFGAKGDGRTDDTKAFQAAWESACNAEGSTVEVPSGSEFLVGPISFSGPNCQPNIV 149
           F+V  FGA GDG  DDT AFQ AW++AC  E   V  P G  F +    FSGP C+P +V
Sbjct: 80  FDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKITSTIFSGP-CKPGLV 139

Query: 150 FQLDGKIIAPTSPSAW--GSGLIQWIEFTKLKGITVKGTGTIDGQGSAWWNDSPTYDPTD 209
           FQLDG ++ P  P  W       QW+ F +L G T  G GT++G G  WW+      P  
Sbjct: 140 FQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWD-----LPCK 199

Query: 210 ENLDKESSSGKLPSTKPTALRFYGSDGVTVTGITIQNSQQAHLKFDSCTSVQVSSLTVSS 269
            +   + SS   P   PT +RF+ S+ + V G+ IQNS Q H+KFD C  V ++ + +SS
Sbjct: 200 PHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISS 259

Query: 270 PGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSAIYIHDVNCGPGHGISIGGLG 329
           P  SPNTDGIHL N+++V I NS ++ GDDCISI TG S + I  V CGP HGISIG LG
Sbjct: 260 PKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGISIGSLG 319

Query: 330 RDQTKACVSNVTVRDTKLQNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVRTPIMIDQYY 389
              ++ACVSN+TVR+T ++++  G+R+KTWQGG+GSV  +LF NIQ+ +V   I++DQYY
Sbjct: 320 VHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNCIIVDQYY 379

Query: 390 CDGGRCHNGSSAVAISGVNYVNIRGTY--TSTPVHLACSDSLPCTGVTLDTIQL--EGSG 449
           C    C N +SAV +  V Y NI+GTY   S P+H ACSD++ CT +T+  ++L  E   
Sbjct: 380 CQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLPEEGE 439

Query: 450 NSNEPFCWKAYGELKTSTVPPIDCLQSGNPSKAQARDS 482
             ++PFCW AYG+ +T T+PPIDCL  G+P   +A DS
Sbjct: 440 LVDDPFCWNAYGKQETLTIPPIDCLLDGSPVVEEAYDS 471

BLAST of Lcy09g019640 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 3.2e-81
Identity = 163/380 (42.89%), Postives = 228/380 (60.00%), Query Frame = 0

Query: 91  NVLDFGAKGDGRTDDTKAFQAAWESACNAEGST-VEVPSGSEFLVGPISFSGPNCQPNIV 150
           +V +FGAKG+G TDDTKAF  AW++AC+++  T + VP     L+ PI  SGP C+  + 
Sbjct: 54  HVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGP-CKARLT 113

Query: 151 FQLDGKIIAPTSPSAW-GSGLIQWIEFTKLKGITVKGTGTIDGQGSAWWNDSPTYDPTDE 210
            Q+ G IIAP  P  W G    +W+ F  L  +TV+G GT++G G  WW          E
Sbjct: 114 LQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWW----------E 173

Query: 211 NLDKESSSGKLPSTKPTALRFYGSDGVTVTGITIQNSQQAHLKFDSCTSVQVSSLTVSSP 270
              K + S       PTAL F+    + V  + + +SQQ H+   SC  V +S L V +P
Sbjct: 174 RSCKHNHSNPCRGA-PTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAP 233

Query: 271 GDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSAIYIHDVNCGPGHGISIGGLGR 330
             SPNTDGIH+  S+ ++I N+ ++ GDDCISI   S+ I I ++ CGPGHGISIG LG+
Sbjct: 234 ATSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGK 293

Query: 331 DQTKACVSNVTVRDTKLQNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVRTPIMIDQYYC 390
            ++   V ++TV    + +T  GVRIKTWQGGSG V  I+F NI++++V  PI+IDQYYC
Sbjct: 294 SKSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYC 353

Query: 391 DGGR-CHNGSSAVAISGVNYVNIRGTYTS-TPVHLACSDSLPCTGVTLDTIQLEGSGNS- 450
           D  + C N +SA++I  +++V++RGT  S   + ++CSDS PC  + L  I LE S    
Sbjct: 354 DSRKPCANQTSAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDG 413

Query: 451 -NEPFCWKAYGELKTSTVPP 465
             E FCW+AYG       PP
Sbjct: 414 FTESFCWEAYGSSSGQVYPP 421

BLAST of Lcy09g019640 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 9.3e-81
Identity = 171/397 (43.07%), Postives = 230/397 (57.93%), Query Frame = 0

Query: 85  DGSGNFNVLDFGAKGDGRTDDTKAFQAAWESACNAEGSTVEVPSGSEFLVGPISFSGPNC 144
           D S   NV DFGAKGDGR DDTKAF+ AW++AC++  S V +     +LV PISFSGP C
Sbjct: 85  DASKTVNVDDFGAKGDGR-DDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGP-C 144

Query: 145 QPNIVFQLDGKIIAPTSPSAWGSGLIQWIEFTKLKGITVKGTGTIDGQGSAWWNDSPTYD 204
           +  +  Q+ G I A    S +      W+ F  ++ + V+G GTI+G G  WW +S    
Sbjct: 145 KSGLTMQIYGTIEASDDRSDYRKDGRHWLVFDSVQNLRVEGGGTINGNGKIWWQNSC--- 204

Query: 205 PTDENLDKESSSGKLP-STKPTALRFYGSDGVTVTGITIQNSQQAHLKFDSCTSVQVSSL 264
                     ++  LP    PTAL FY S  V V  + I+N+QQ H+ FD+C +VQ S+L
Sbjct: 205 ---------KTNKALPCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNL 264

Query: 265 TVSSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSAIYIHDVNCGPGHGISI 324
            V++P +SPNTDGIH+  +QN+ IS+  +  GDDCISI  GS  + ++D+ CGPGHGISI
Sbjct: 265 MVTAPENSPNTDGIHVTGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISI 324

Query: 325 GGLGRDQTKACVSNVTVRDTKLQNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVRTPIMI 384
           G LG   ++A VS+V V   KL  T  GVRIKTWQGGSGS   I F N+++ +V  PI+I
Sbjct: 325 GSLGYGNSEAHVSDVVVNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIII 384

Query: 385 DQYYCDGGR-CHNGSSAVAISGVNYVNIRGTYTS-TPVHLACSDSLPCTGVTLDTIQLEG 444
           DQ YCD  + C   SSAV +  V Y NI+GT  S   +   CS   PC G+ L+ + LE 
Sbjct: 385 DQNYCDQDKPCQEQSSAVQVKNVVYQNIKGTCASNVAITFDCSKRFPCQGIVLEDVDLEI 444

Query: 445 SGNSNEPFCWKAYGELKTSTVPPIDCLQSGNPSKAQA 479
            G +            +T  V P  C + G   + +A
Sbjct: 445 EGGAAAKALCNNVELSETGVVSP-HCQEEGGEEEEEA 466

BLAST of Lcy09g019640 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 298.5 bits (763), Expect = 1.3e-79
Identity = 159/356 (44.66%), Postives = 222/356 (62.36%), Query Frame = 0

Query: 91  NVLDFGAKGDGRTDDTKAFQAAWESACNAEGS-TVEVPSGSEFLVGPISFSGPNCQPNIV 150
           +V DFGAKGDG+TDDT+AF  AW+ AC++ G+  + VP G+ +L+  I  +GP C   + 
Sbjct: 69  SVSDFGAKGDGKTDDTQAFVNAWKKACSSNGAVNLLVPKGNTYLLKSIQLTGP-CNSILT 128

Query: 151 FQLDGKIIAPTSPSAWGSGLIQWIEFTKLKGITVKG--TGTIDGQGSAWWNDSPTYDPTD 210
            Q+ G + A    S +   + +WI F  +  ++V G  TG +DG G  WW +S       
Sbjct: 129 VQIFGTLSASQKRSDY-KDISKWIMFDGVNNLSVDGGDTGVVDGNGETWWQNS-----CK 188

Query: 211 ENLDKESSSGKLPSTK-PTALRFYGSDGVTVTGITIQNSQQAHLKFDSCTSVQVSSLTVS 270
            N  K       P TK PTAL FY S  + V  + ++N+QQ  +  + C++VQVS++ V+
Sbjct: 189 RNKAK-------PCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVT 248

Query: 271 SPGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSAIYIHDVNCGPGHGISIGGL 330
           +P DSPNTDGIH+ N+QN+ +S S +  GDDCISI++GS  + I+D+ CGPGHGISIG L
Sbjct: 249 APADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSL 308

Query: 331 GRDQTKACVSNVTVRDTKLQNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVRTPIMIDQY 390
           G D +KA VS VTV   KL  T  GVRIKT+QGGSG+   I+F NIQ+ +V+ PI+IDQ 
Sbjct: 309 GDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQD 368

Query: 391 YCDGGRCHNGSSAVAISGVNYVNIRGTYTS-TPVHLACSDSLPCTGVTLDTIQLEG 442
           YCD  +C    SAV +  V Y +I GT  S   +   CS + PC G+ LD + ++G
Sbjct: 369 YCDKSKCTTEKSAVQVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDRVNIKG 410

BLAST of Lcy09g019640 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 3.7e-77
Identity = 151/358 (42.18%), Postives = 217/358 (60.61%), Query Frame = 0

Query: 85  DGSGNFNVLDFGAKGDGRTDDTKAFQAAWESACNAEGSTVEVPSGSEFLVGPISFSGPNC 144
           D    FNV  +GA GDG+ D T+AF  AW++AC    + + VP   +F+V  + F+GP C
Sbjct: 54  DAINIFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGP-C 113

Query: 145 QPNIVFQLDGKIIAPTSPSAWGSGLIQWIEFTKLKGITVKGTGTIDGQGSAWWNDSPTYD 204
           QP+  F++DG I A  +P++W +  I W++F KL G T+ G G IDGQG  WW     + 
Sbjct: 114 QPHFTFKVDGIIAAYQNPASWKNNRI-WLQFAKLTGFTLMGKGVIDGQGKQWWAGQCKWV 173

Query: 205 PTDENLDKESSSGKLPSTKPTALRFYGSDGVTVTGITIQNSQQAHLKFDSCTSVQVSSLT 264
              E  +           +PTA++F  S G+ + G+ + NS + HL F +C  V++  ++
Sbjct: 174 NGREICNDRD--------RPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGIS 233

Query: 265 VSSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSAIYIHDVNCGPGHGISIG 324
           +++P DSPNTDGI +  S+N  +  + +  GDDC++I TGSS I I D+ CGPGHGISIG
Sbjct: 234 ITAPRDSPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIG 293

Query: 325 GLGRDQTKACVSNVTVRDTKLQNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVRTPIMID 384
            LGR+ ++A VS V V   K  +T  G+RIKTWQGGSG    I++ N+++ +   PI+I+
Sbjct: 294 SLGRENSRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILIN 353

Query: 385 QYYC-DGGRCHNGSSAVAISGVNYVNIRGT-YTSTPVHLACSDSLPCTGVTLDTIQLE 441
           Q+YC     C N  SAV I  V Y NIRGT  T+  + L CSDS+PC  + L  I L+
Sbjct: 354 QFYCTSASACQNQRSAVQIQDVTYKNIRGTSATAAAIQLKCSDSMPCKDIKLSDISLK 401

BLAST of Lcy09g019640 vs. ExPASy TrEMBL
Match: A0A6J1F9Z9 (polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111442159 PE=3 SV=1)

HSP 1 Score: 906.4 bits (2341), Expect = 5.2e-260
Identity = 451/487 (92.61%), Postives = 461/487 (94.66%), Query Frame = 0

Query: 1   MEMGGKNLKCLATIAFICVFVWSFSLQNCHAREGRHWGAHRKTGGYRHRHAHRHRGGSGS 60
           MEMGGKNLKCLATIAFIC FVWSFSLQNC AREG+HW AHRKTGGYRH   HR RGGSGS
Sbjct: 1   MEMGGKNLKCLATIAFICAFVWSFSLQNCQAREGKHW-AHRKTGGYRHGRIHRDRGGSGS 60

Query: 61  SRLMGSG-----DVVGASLASKGGASKGGDGSGNFNVLDFGAKGDGRTDDTKAFQAAWES 120
           SRL GSG     DVVGASLASKG ASKG  GSGNFNVLDFGAKGDGR DDTKAFQAAWES
Sbjct: 61  SRLTGSGHVGSEDVVGASLASKGDASKG--GSGNFNVLDFGAKGDGRADDTKAFQAAWES 120

Query: 121 ACNAEGSTVEVPSGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSPSAWGSGLIQWIEF 180
           AC+AE STVEVPSGSEFLVGPISFSGPNC+PNIVFQLDGKIIAPTSPSAWGSGL+QWIEF
Sbjct: 121 ACSAEKSTVEVPSGSEFLVGPISFSGPNCKPNIVFQLDGKIIAPTSPSAWGSGLLQWIEF 180

Query: 181 TKLKGITVKGTGTIDGQGSAWWNDSPTYDPTDENLDKESSSGKLPSTKPTALRFYGSDGV 240
           TKLKGITVKGTGTIDGQGS WWNDSPTYDPTDENLDKESSSGKLPSTKPTALRFYGSDGV
Sbjct: 181 TKLKGITVKGTGTIDGQGSVWWNDSPTYDPTDENLDKESSSGKLPSTKPTALRFYGSDGV 240

Query: 241 TVTGITIQNSQQAHLKFDSCTSVQVSSLTVSSPGDSPNTDGIHLQNSQNVIISNSNLACG 300
           TV+GITIQNSQQAHLKFDSCTSVQVSSLT+SSPGDSPNTDGIHLQNSQNVIISNSNLACG
Sbjct: 241 TVSGITIQNSQQAHLKFDSCTSVQVSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLACG 300

Query: 301 DDCISIQTGSSAIYIHDVNCGPGHGISIGGLGRDQTKACVSNVTVRDTKLQNTMTGVRIK 360
           DDCISIQTGSSA+YIH+VNCGPGHGISIGGLGRDQTKACVSNVTVRD KLQNTM GVRIK
Sbjct: 301 DDCISIQTGSSAVYIHNVNCGPGHGISIGGLGRDQTKACVSNVTVRDVKLQNTMNGVRIK 360

Query: 361 TWQGGSGSVQGILFSNIQVSDVRTPIMIDQYYCDGGRCHNGSSAVAISGVNYVNIRGTYT 420
           TWQGGSG VQGILFSNIQVSDV+TPIMIDQYYCDGG CHNGSSAVAISGVNYVNIRGTYT
Sbjct: 361 TWQGGSGLVQGILFSNIQVSDVKTPIMIDQYYCDGGSCHNGSSAVAISGVNYVNIRGTYT 420

Query: 421 STPVHLACSDSLPCTGVTLDTIQLEGSGNSNEPFCWKAYGELKTSTVPPIDCLQSGNPSK 480
           STPVHLACSDSLPCTGVTLDTIQLEGSG+SNEPFCWKAYGELKTSTVP IDCLQSGNP K
Sbjct: 421 STPVHLACSDSLPCTGVTLDTIQLEGSGHSNEPFCWKAYGELKTSTVPAIDCLQSGNPGK 480

Query: 481 AQARDSC 483
           AQA DSC
Sbjct: 481 AQAHDSC 484

BLAST of Lcy09g019640 vs. ExPASy TrEMBL
Match: A0A6J1J1T6 (polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111481937 PE=3 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 1.7e-255
Identity = 444/487 (91.17%), Postives = 456/487 (93.63%), Query Frame = 0

Query: 1   MEMGGKNLKCLATIAFICVFVWSFSLQNCHAREGRHWGAHRKTGGYRHRHAHRHRGGSGS 60
           MEMGGKNLKCLATIAFIC FVWSFSLQNC AR G+HW AHRKTGGYRH   HRHRGGS S
Sbjct: 1   MEMGGKNLKCLATIAFICAFVWSFSLQNCQARGGKHW-AHRKTGGYRHGRIHRHRGGSSS 60

Query: 61  SRL-----MGSGDVVGASLASKGGASKGGDGSGNFNVLDFGAKGDGRTDDTKAFQAAWES 120
             L     +GS DVVGASLASKG ASKG  GSGNFNVLDFGAKGDGR DDTKAFQAAWES
Sbjct: 61  LGLTDSGHVGSEDVVGASLASKGDASKG--GSGNFNVLDFGAKGDGRADDTKAFQAAWES 120

Query: 121 ACNAEGSTVEVPSGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSPSAWGSGLIQWIEF 180
           ACNAE STVEVPSGSEFLVGPISFSGPNC+PNIVFQLDGKIIAPTSPSAWGSGL+QWIEF
Sbjct: 121 ACNAEKSTVEVPSGSEFLVGPISFSGPNCKPNIVFQLDGKIIAPTSPSAWGSGLLQWIEF 180

Query: 181 TKLKGITVKGTGTIDGQGSAWWNDSPTYDPTDENLDKESSSGKLPSTKPTALRFYGSDGV 240
           TKLKGITVKGTGTIDGQGS WWNDSPTYDPTDENLDKESSS KLPSTKPTALRFYGSDGV
Sbjct: 181 TKLKGITVKGTGTIDGQGSVWWNDSPTYDPTDENLDKESSSRKLPSTKPTALRFYGSDGV 240

Query: 241 TVTGITIQNSQQAHLKFDSCTSVQVSSLTVSSPGDSPNTDGIHLQNSQNVIISNSNLACG 300
           TV+GITIQNSQQAHLKFDSCTSVQVSSLT+SSPGDSPNTDGIHLQNSQNVIISNSNLACG
Sbjct: 241 TVSGITIQNSQQAHLKFDSCTSVQVSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLACG 300

Query: 301 DDCISIQTGSSAIYIHDVNCGPGHGISIGGLGRDQTKACVSNVTVRDTKLQNTMTGVRIK 360
           DDCISIQTGSSA+YIH+VNCGPGHGISIGGLGRDQTKACVSNVTVRD KLQNTM GVRIK
Sbjct: 301 DDCISIQTGSSAVYIHNVNCGPGHGISIGGLGRDQTKACVSNVTVRDVKLQNTMNGVRIK 360

Query: 361 TWQGGSGSVQGILFSNIQVSDVRTPIMIDQYYCDGGRCHNGSSAVAISGVNYVNIRGTYT 420
           TWQGGSG VQGILFSNIQVSDV+TPIMIDQYYCDGG CHNGSSAVAISGVNYVNIRGTYT
Sbjct: 361 TWQGGSGLVQGILFSNIQVSDVKTPIMIDQYYCDGGSCHNGSSAVAISGVNYVNIRGTYT 420

Query: 421 STPVHLACSDSLPCTGVTLDTIQLEGSGNSNEPFCWKAYGELKTSTVPPIDCLQSGNPSK 480
           STPVHLACSDSLPCTGVTLDTIQLEGSG SNEPFCWKAYGELKTSTVP I+CLQSGNP K
Sbjct: 421 STPVHLACSDSLPCTGVTLDTIQLEGSGRSNEPFCWKAYGELKTSTVPAIECLQSGNPGK 480

Query: 481 AQARDSC 483
           AQ+ +SC
Sbjct: 481 AQSHNSC 484

BLAST of Lcy09g019640 vs. ExPASy TrEMBL
Match: A0A6J1EJN2 (polygalacturonase At1g48100 OS=Cucurbita moschata OX=3662 GN=LOC111435099 PE=3 SV=1)

HSP 1 Score: 861.3 bits (2224), Expect = 1.9e-246
Identity = 429/483 (88.82%), Postives = 440/483 (91.10%), Query Frame = 0

Query: 1   MEMGGKNLKCLATIAFICVFVWSFSLQNCHAREGRHWGAHRKTGGYRHRHAHRHRGGSGS 60
           M+M GKNLKCLATIAFICVFVWSFSL+NCH REGR WGAHRKTGGYRH H H        
Sbjct: 1   MKMCGKNLKCLATIAFICVFVWSFSLENCHGREGRRWGAHRKTGGYRHHHVH-------- 60

Query: 61  SRLMGSGDVVGASLASKGGASKGGDGSGNFNVLDFGAKGDGRTDDTKAFQAAWESACNAE 120
              + S DVVGASLAS+G       GSG+FNVLDFGAKGDG  DDTKAFQAAWESACNAE
Sbjct: 61  ---VDSEDVVGASLASEG-------GSGSFNVLDFGAKGDGEEDDTKAFQAAWESACNAE 120

Query: 121 GSTVEVPSGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSPSAWGSGLIQWIEFTKLKG 180
           GS VEVPSG EFLVGPISFSGPNCQPNIVFQLDGKIIAPTSPSAWGSGL+QWIEFTKLKG
Sbjct: 121 GSMVEVPSGYEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSPSAWGSGLLQWIEFTKLKG 180

Query: 181 ITVKGTGTIDGQGSAWW-NDSPTYDPTDENLDKESSSGKLPSTKPTALRFYGSDGVTVTG 240
           ITVKGTGTIDGQGS WW +DSPT DPTDENLDKESSSG+LP TK TALRFYGSDGVTVTG
Sbjct: 181 ITVKGTGTIDGQGSVWWTDDSPTDDPTDENLDKESSSGRLPRTKLTALRFYGSDGVTVTG 240

Query: 241 ITIQNSQQAHLKFDSCTSVQVSSLTVSSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCI 300
           ITIQNSQQAHLKFDSCTSV VSSLT+SSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCI
Sbjct: 241 ITIQNSQQAHLKFDSCTSVPVSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCI 300

Query: 301 SIQTGSSAIYIHDVNCGPGHGISIGGLGRDQTKACVSNVTVRDTKLQNTMTGVRIKTWQG 360
           SIQTGSS+IYIHDVNCGPGHGISIGGLGRDQTKACVSNVTVRD KLQNTM GVRIKTWQG
Sbjct: 301 SIQTGSSSIYIHDVNCGPGHGISIGGLGRDQTKACVSNVTVRDVKLQNTMNGVRIKTWQG 360

Query: 361 GSGSVQGILFSNIQVSDVRTPIMIDQYYCDGGRCHNGSSAVAISGVNYVNIRGTYTSTPV 420
           GSGSVQGILFSNIQVSDV TP+MIDQYYCDGGRCHNGSSAVAISGVNYVNIRGTYTSTPV
Sbjct: 361 GSGSVQGILFSNIQVSDVGTPVMIDQYYCDGGRCHNGSSAVAISGVNYVNIRGTYTSTPV 420

Query: 421 HLACSDSLPCTGVTLDTIQLEGSGNSNEPFCWKAYGELKTSTVPPIDCLQSGNPSKAQAR 480
           HLACSDSLPCTGVTLDTIQLEGS  SNEPFCWKAYGELKTSTVPPIDCLQSGNPSKAQA 
Sbjct: 421 HLACSDSLPCTGVTLDTIQLEGSSRSNEPFCWKAYGELKTSTVPPIDCLQSGNPSKAQAH 465

Query: 481 DSC 483
             C
Sbjct: 481 GFC 465

BLAST of Lcy09g019640 vs. ExPASy TrEMBL
Match: A0A6J1JNK8 (polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111486313 PE=3 SV=1)

HSP 1 Score: 858.6 bits (2217), Expect = 1.2e-245
Identity = 426/483 (88.20%), Postives = 440/483 (91.10%), Query Frame = 0

Query: 1   MEMGGKNLKCLATIAFICVFVWSFSLQNCHAREGRHWGAHRKTGGYRHRHAHRHRGGSGS 60
           M+M GKNLKCLATIAFIC+FVWSFSL+NCH REGR WGAHRKTGGYRH H H        
Sbjct: 1   MKMCGKNLKCLATIAFICIFVWSFSLENCHGREGRRWGAHRKTGGYRHHHVH-------- 60

Query: 61  SRLMGSGDVVGASLASKGGASKGGDGSGNFNVLDFGAKGDGRTDDTKAFQAAWESACNAE 120
              + S DVVG+SLAS+G       GSG+FNVLDFGAKGDG  DDTKAFQAAWESACNAE
Sbjct: 61  ---VESEDVVGSSLASEG-------GSGSFNVLDFGAKGDGEEDDTKAFQAAWESACNAE 120

Query: 121 GSTVEVPSGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSPSAWGSGLIQWIEFTKLKG 180
           GS VEVPSG EFLVGPISFSGPNCQPNIVFQLDGKIIAPTSPSAWGSGL+QWIEFTKLKG
Sbjct: 121 GSMVEVPSGYEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSPSAWGSGLLQWIEFTKLKG 180

Query: 181 ITVKGTGTIDGQGSAWW-NDSPTYDPTDENLDKESSSGKLPSTKPTALRFYGSDGVTVTG 240
           ITVKGTGTIDGQGS WW +DSPT DPTDENLDKESSSG+LP TKPTALRFYGSDGVTVTG
Sbjct: 181 ITVKGTGTIDGQGSVWWSDDSPTDDPTDENLDKESSSGRLPRTKPTALRFYGSDGVTVTG 240

Query: 241 ITIQNSQQAHLKFDSCTSVQVSSLTVSSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCI 300
           ITIQNSQQAHLKFDSCTSVQVSSLT+SSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCI
Sbjct: 241 ITIQNSQQAHLKFDSCTSVQVSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCI 300

Query: 301 SIQTGSSAIYIHDVNCGPGHGISIGGLGRDQTKACVSNVTVRDTKLQNTMTGVRIKTWQG 360
           SIQTGSS+IYIHDVNCGPGHGISIGGLGRDQTKACVSNVTVRD KLQNTM GVRIKTWQG
Sbjct: 301 SIQTGSSSIYIHDVNCGPGHGISIGGLGRDQTKACVSNVTVRDVKLQNTMNGVRIKTWQG 360

Query: 361 GSGSVQGILFSNIQVSDVRTPIMIDQYYCDGGRCHNGSSAVAISGVNYVNIRGTYTSTPV 420
           GSGSV GILFSNIQVSDV TP+ IDQYYCDGGRCHNGSSAVAISGVNYVNIRGTYTS PV
Sbjct: 361 GSGSVLGILFSNIQVSDVGTPVKIDQYYCDGGRCHNGSSAVAISGVNYVNIRGTYTSRPV 420

Query: 421 HLACSDSLPCTGVTLDTIQLEGSGNSNEPFCWKAYGELKTSTVPPIDCLQSGNPSKAQAR 480
           HLACSDSLPCTGVTLDTIQLEGS +SNEPFCWKAYGELKTSTVPPIDCLQSGNPSKAQA 
Sbjct: 421 HLACSDSLPCTGVTLDTIQLEGSSHSNEPFCWKAYGELKTSTVPPIDCLQSGNPSKAQAH 465

Query: 481 DSC 483
             C
Sbjct: 481 GFC 465

BLAST of Lcy09g019640 vs. ExPASy TrEMBL
Match: A0A1S3B8B8 (polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103487102 PE=3 SV=1)

HSP 1 Score: 846.3 bits (2185), Expect = 6.4e-242
Identity = 423/488 (86.68%), Postives = 439/488 (89.96%), Query Frame = 0

Query: 1   MEMGGKNLKCLATIAFICVFVWSFSLQNCHAREGRHWGAHRKTGGYRHRHAHRHRGGSGS 60
           MEMG KNLKCL TIAFICVFVWSFSL NCHA         RKT GYRH   HRH  GSG 
Sbjct: 1   MEMGRKNLKCLTTIAFICVFVWSFSLHNCHA---------RKTVGYRHWQVHRHHQGSGR 60

Query: 61  S-RLMGSG-----DVVGASLASKGGASKGGDGSGNFNVLDFGAKGDGRTDDTKAFQAAWE 120
           S RL GSG     DVVG SLASKG A+ G  GSGNFNVLDFGAKGDG TDDTKAFQ+AWE
Sbjct: 61  SLRLTGSGHVGSEDVVGTSLASKGRATNG--GSGNFNVLDFGAKGDGETDDTKAFQSAWE 120

Query: 121 SACNAEGSTVEVPSGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSPSAWGSGLIQWIE 180
           SACN EGS VEVPSGS FLVGPISFSGPNCQPNI+FQLDGKIIAPTSPSAWGSG++QWIE
Sbjct: 121 SACNVEGSVVEVPSGSNFLVGPISFSGPNCQPNIMFQLDGKIIAPTSPSAWGSGILQWIE 180

Query: 181 FTKLKGITVKGTGTIDGQGSAWWNDSPTYDPTDENLDKESSSGKLPSTKPTALRFYGSDG 240
           FTKLKGITVKGTGTIDGQGS WWNDSPT +PTDENLDKESS GKLPSTKPTALRFYGSD 
Sbjct: 181 FTKLKGITVKGTGTIDGQGSVWWNDSPTNNPTDENLDKESSGGKLPSTKPTALRFYGSDE 240

Query: 241 VTVTGITIQNSQQAHLKFDSCTSVQVSSLTVSSPGDSPNTDGIHLQNSQNVIISNSNLAC 300
           VTV+GITIQNSQ+AHLKFDSCT+VQVSSLT+SSPGDSPNTDGIHLQNSQNVIISNSNLAC
Sbjct: 241 VTVSGITIQNSQKAHLKFDSCTAVQVSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLAC 300

Query: 301 GDDCISIQTGSSAIYIHDVNCGPGHGISIGGLGRDQTKACVSNVTVRDTKLQNTMTGVRI 360
           GDDCISIQTGSS +YIH+VNCGPGHGISIGGLGRD+TKACVSN+TVRD KLQNTM GVRI
Sbjct: 301 GDDCISIQTGSSGVYIHNVNCGPGHGISIGGLGRDETKACVSNITVRDVKLQNTMNGVRI 360

Query: 361 KTWQGGSGSVQGILFSNIQVSDVRTPIMIDQYYCDGGRCHNGSSAVAISGVNYVNIRGTY 420
           KTWQGGSGSVQGILFSNIQV+DVR PIMIDQYYCDGGRCHNGSSAVAISGVNYVNIRGTY
Sbjct: 361 KTWQGGSGSVQGILFSNIQVTDVRIPIMIDQYYCDGGRCHNGSSAVAISGVNYVNIRGTY 420

Query: 421 TSTPVHLACSDSLPCTGVTLDTIQLEGSGNSNEPFCWKAYGELKTSTVPPIDCLQSGNPS 480
           TSTPV  ACSDSLPCTGVTLDTIQLEGS  SNEPFCWKAYGELKTSTVPP+DCLQSGNP 
Sbjct: 421 TSTPVRFACSDSLPCTGVTLDTIQLEGSDGSNEPFCWKAYGELKTSTVPPVDCLQSGNPF 477

Query: 481 KAQARDSC 483
           KAQA DSC
Sbjct: 481 KAQAHDSC 477

BLAST of Lcy09g019640 vs. NCBI nr
Match: XP_023527919.1 (polygalacturonase At1g48100-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 906.4 bits (2341), Expect = 1.1e-259
Identity = 451/487 (92.61%), Postives = 459/487 (94.25%), Query Frame = 0

Query: 1   MEMGGKNLKCLATIAFICVFVWSFSLQNCHAREGRHWGAHRKTGGYRHRHAHRHRGGSGS 60
           MEMGGKNLKCLATIAFIC FVWSFSLQNC AR G+HW AHRKTGGYRH H HRHRGGS S
Sbjct: 1   MEMGGKNLKCLATIAFICAFVWSFSLQNCQARGGKHW-AHRKTGGYRHGHIHRHRGGSSS 60

Query: 61  SRLMGSG-----DVVGASLASKGGASKGGDGSGNFNVLDFGAKGDGRTDDTKAFQAAWES 120
           S LM SG     DVVGASLASKG ASKG  GSGNFNVLDFGAKGDGR DDTKAFQAAWES
Sbjct: 61  SGLMDSGHVGSEDVVGASLASKGDASKG--GSGNFNVLDFGAKGDGRADDTKAFQAAWES 120

Query: 121 ACNAEGSTVEVPSGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSPSAWGSGLIQWIEF 180
           ACNAE STVEVPSGSEFLVGPISFSGPNC+PNIVFQLDGKIIAPTSPSAWGSGL+QWIEF
Sbjct: 121 ACNAEKSTVEVPSGSEFLVGPISFSGPNCKPNIVFQLDGKIIAPTSPSAWGSGLLQWIEF 180

Query: 181 TKLKGITVKGTGTIDGQGSAWWNDSPTYDPTDENLDKESSSGKLPSTKPTALRFYGSDGV 240
           TKLKGITVKGTGTIDGQGS WWNDSPTYDPTDENLDKESSSGKLPSTKPTALRFYGSDGV
Sbjct: 181 TKLKGITVKGTGTIDGQGSVWWNDSPTYDPTDENLDKESSSGKLPSTKPTALRFYGSDGV 240

Query: 241 TVTGITIQNSQQAHLKFDSCTSVQVSSLTVSSPGDSPNTDGIHLQNSQNVIISNSNLACG 300
           TV+GITIQNSQQAHLKFDSCTSVQVSSLT+SSPGDSPNTDGIHLQNSQNVIISNSNLACG
Sbjct: 241 TVSGITIQNSQQAHLKFDSCTSVQVSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLACG 300

Query: 301 DDCISIQTGSSAIYIHDVNCGPGHGISIGGLGRDQTKACVSNVTVRDTKLQNTMTGVRIK 360
           DDCISIQTGSSA+YIH+VNCGPGHGISIGGLGRDQTKACVSNVTVRD KLQNTM GVRIK
Sbjct: 301 DDCISIQTGSSAVYIHNVNCGPGHGISIGGLGRDQTKACVSNVTVRDVKLQNTMNGVRIK 360

Query: 361 TWQGGSGSVQGILFSNIQVSDVRTPIMIDQYYCDGGRCHNGSSAVAISGVNYVNIRGTYT 420
           TWQGGSG VQGILFSNIQVSDV+TPIMIDQYYCDGG CHNGSSAVAISGVNYVNIRGTYT
Sbjct: 361 TWQGGSGLVQGILFSNIQVSDVKTPIMIDQYYCDGGSCHNGSSAVAISGVNYVNIRGTYT 420

Query: 421 STPVHLACSDSLPCTGVTLDTIQLEGSGNSNEPFCWKAYGELKTSTVPPIDCLQSGNPSK 480
           STPVHLACSDSLPCTGVTLDTIQLEGSG SNEPFCWKAYGELKTSTVP IDCLQSGNP K
Sbjct: 421 STPVHLACSDSLPCTGVTLDTIQLEGSGRSNEPFCWKAYGELKTSTVPAIDCLQSGNPGK 480

Query: 481 AQARDSC 483
           AQA DSC
Sbjct: 481 AQAHDSC 484

BLAST of Lcy09g019640 vs. NCBI nr
Match: XP_022935210.1 (polygalacturonase At1g48100-like [Cucurbita moschata])

HSP 1 Score: 906.4 bits (2341), Expect = 1.1e-259
Identity = 451/487 (92.61%), Postives = 461/487 (94.66%), Query Frame = 0

Query: 1   MEMGGKNLKCLATIAFICVFVWSFSLQNCHAREGRHWGAHRKTGGYRHRHAHRHRGGSGS 60
           MEMGGKNLKCLATIAFIC FVWSFSLQNC AREG+HW AHRKTGGYRH   HR RGGSGS
Sbjct: 1   MEMGGKNLKCLATIAFICAFVWSFSLQNCQAREGKHW-AHRKTGGYRHGRIHRDRGGSGS 60

Query: 61  SRLMGSG-----DVVGASLASKGGASKGGDGSGNFNVLDFGAKGDGRTDDTKAFQAAWES 120
           SRL GSG     DVVGASLASKG ASKG  GSGNFNVLDFGAKGDGR DDTKAFQAAWES
Sbjct: 61  SRLTGSGHVGSEDVVGASLASKGDASKG--GSGNFNVLDFGAKGDGRADDTKAFQAAWES 120

Query: 121 ACNAEGSTVEVPSGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSPSAWGSGLIQWIEF 180
           AC+AE STVEVPSGSEFLVGPISFSGPNC+PNIVFQLDGKIIAPTSPSAWGSGL+QWIEF
Sbjct: 121 ACSAEKSTVEVPSGSEFLVGPISFSGPNCKPNIVFQLDGKIIAPTSPSAWGSGLLQWIEF 180

Query: 181 TKLKGITVKGTGTIDGQGSAWWNDSPTYDPTDENLDKESSSGKLPSTKPTALRFYGSDGV 240
           TKLKGITVKGTGTIDGQGS WWNDSPTYDPTDENLDKESSSGKLPSTKPTALRFYGSDGV
Sbjct: 181 TKLKGITVKGTGTIDGQGSVWWNDSPTYDPTDENLDKESSSGKLPSTKPTALRFYGSDGV 240

Query: 241 TVTGITIQNSQQAHLKFDSCTSVQVSSLTVSSPGDSPNTDGIHLQNSQNVIISNSNLACG 300
           TV+GITIQNSQQAHLKFDSCTSVQVSSLT+SSPGDSPNTDGIHLQNSQNVIISNSNLACG
Sbjct: 241 TVSGITIQNSQQAHLKFDSCTSVQVSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLACG 300

Query: 301 DDCISIQTGSSAIYIHDVNCGPGHGISIGGLGRDQTKACVSNVTVRDTKLQNTMTGVRIK 360
           DDCISIQTGSSA+YIH+VNCGPGHGISIGGLGRDQTKACVSNVTVRD KLQNTM GVRIK
Sbjct: 301 DDCISIQTGSSAVYIHNVNCGPGHGISIGGLGRDQTKACVSNVTVRDVKLQNTMNGVRIK 360

Query: 361 TWQGGSGSVQGILFSNIQVSDVRTPIMIDQYYCDGGRCHNGSSAVAISGVNYVNIRGTYT 420
           TWQGGSG VQGILFSNIQVSDV+TPIMIDQYYCDGG CHNGSSAVAISGVNYVNIRGTYT
Sbjct: 361 TWQGGSGLVQGILFSNIQVSDVKTPIMIDQYYCDGGSCHNGSSAVAISGVNYVNIRGTYT 420

Query: 421 STPVHLACSDSLPCTGVTLDTIQLEGSGNSNEPFCWKAYGELKTSTVPPIDCLQSGNPSK 480
           STPVHLACSDSLPCTGVTLDTIQLEGSG+SNEPFCWKAYGELKTSTVP IDCLQSGNP K
Sbjct: 421 STPVHLACSDSLPCTGVTLDTIQLEGSGHSNEPFCWKAYGELKTSTVPAIDCLQSGNPGK 480

Query: 481 AQARDSC 483
           AQA DSC
Sbjct: 481 AQAHDSC 484

BLAST of Lcy09g019640 vs. NCBI nr
Match: XP_038905486.1 (polygalacturonase At1g48100 [Benincasa hispida])

HSP 1 Score: 901.4 bits (2328), Expect = 3.5e-258
Identity = 441/487 (90.55%), Postives = 460/487 (94.46%), Query Frame = 0

Query: 1   MEMGGKNLKCLATIAFICVFVWSFSLQNCHAREGRHWGAHRKTGGYRHRHAHRHRGGSGS 60
           MEMGGKNLKCLA IAFICVFVWSFSLQN HAREG+HW AHR+TGGYRHRH HRHRG SGS
Sbjct: 1   MEMGGKNLKCLAIIAFICVFVWSFSLQNSHAREGKHW-AHRQTGGYRHRHVHRHRGSSGS 60

Query: 61  SRLMGSG-----DVVGASLASKGGASKGGDGSGNFNVLDFGAKGDGRTDDTKAFQAAWES 120
           SRL G+G     DVVG SLAS+G  SK   GSGNFNVLDFGAKGDG+ DDTKAFQ+AW+S
Sbjct: 61  SRLKGNGHVGSEDVVGTSLASEGSDSK--SGSGNFNVLDFGAKGDGKADDTKAFQSAWQS 120

Query: 121 ACNAEGSTVEVPSGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSPSAWGSGLIQWIEF 180
           ACNAEGSTVEVPSGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSPS WGS L+QWIEF
Sbjct: 121 ACNAEGSTVEVPSGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSPSVWGSSLLQWIEF 180

Query: 181 TKLKGITVKGTGTIDGQGSAWWNDSPTYDPTDENLDKESSSGKLPSTKPTALRFYGSDGV 240
           TKLKGITVKGTGTIDGQGS WWNDSPTYDPTDENLDKESSSGKLPSTKPTALRFYGSDGV
Sbjct: 181 TKLKGITVKGTGTIDGQGSVWWNDSPTYDPTDENLDKESSSGKLPSTKPTALRFYGSDGV 240

Query: 241 TVTGITIQNSQQAHLKFDSCTSVQVSSLTVSSPGDSPNTDGIHLQNSQNVIISNSNLACG 300
            V+GITIQNSQQAHLKFDSCTSVQVSSLT+SSPGDSPNTDGIHLQNSQNVIIS+SNLACG
Sbjct: 241 IVSGITIQNSQQAHLKFDSCTSVQVSSLTISSPGDSPNTDGIHLQNSQNVIISHSNLACG 300

Query: 301 DDCISIQTGSSAIYIHDVNCGPGHGISIGGLGRDQTKACVSNVTVRDTKLQNTMTGVRIK 360
           DDCISIQTGSS++YIHDVNCGPGHGISIGGLGRDQTKACVSNVTVRD KLQNTMTGVRIK
Sbjct: 301 DDCISIQTGSSSVYIHDVNCGPGHGISIGGLGRDQTKACVSNVTVRDVKLQNTMTGVRIK 360

Query: 361 TWQGGSGSVQGILFSNIQVSDVRTPIMIDQYYCDGGRCHNGSSAVAISGVNYVNIRGTYT 420
           TWQGGSGSVQGILFSN+QVSDVRTPI+IDQYYCDGGRCHNGSSAVAISGV+YVNI+GTYT
Sbjct: 361 TWQGGSGSVQGILFSNVQVSDVRTPIIIDQYYCDGGRCHNGSSAVAISGVSYVNIKGTYT 420

Query: 421 STPVHLACSDSLPCTGVTLDTIQLEGSGNSNEPFCWKAYGELKTSTVPPIDCLQSGNPSK 480
           STPVH ACSDSLPCTGVTLDTIQLEGSG SNEPFCWKAYGELKTST+PP+DCLQSGNP K
Sbjct: 421 STPVHFACSDSLPCTGVTLDTIQLEGSGRSNEPFCWKAYGELKTSTIPPVDCLQSGNPMK 480

Query: 481 AQARDSC 483
           AQA DSC
Sbjct: 481 AQAHDSC 484

BLAST of Lcy09g019640 vs. NCBI nr
Match: XP_022983321.1 (polygalacturonase At1g48100-like [Cucurbita maxima])

HSP 1 Score: 891.3 bits (2302), Expect = 3.6e-255
Identity = 444/487 (91.17%), Postives = 456/487 (93.63%), Query Frame = 0

Query: 1   MEMGGKNLKCLATIAFICVFVWSFSLQNCHAREGRHWGAHRKTGGYRHRHAHRHRGGSGS 60
           MEMGGKNLKCLATIAFIC FVWSFSLQNC AR G+HW AHRKTGGYRH   HRHRGGS S
Sbjct: 1   MEMGGKNLKCLATIAFICAFVWSFSLQNCQARGGKHW-AHRKTGGYRHGRIHRHRGGSSS 60

Query: 61  SRL-----MGSGDVVGASLASKGGASKGGDGSGNFNVLDFGAKGDGRTDDTKAFQAAWES 120
             L     +GS DVVGASLASKG ASKG  GSGNFNVLDFGAKGDGR DDTKAFQAAWES
Sbjct: 61  LGLTDSGHVGSEDVVGASLASKGDASKG--GSGNFNVLDFGAKGDGRADDTKAFQAAWES 120

Query: 121 ACNAEGSTVEVPSGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSPSAWGSGLIQWIEF 180
           ACNAE STVEVPSGSEFLVGPISFSGPNC+PNIVFQLDGKIIAPTSPSAWGSGL+QWIEF
Sbjct: 121 ACNAEKSTVEVPSGSEFLVGPISFSGPNCKPNIVFQLDGKIIAPTSPSAWGSGLLQWIEF 180

Query: 181 TKLKGITVKGTGTIDGQGSAWWNDSPTYDPTDENLDKESSSGKLPSTKPTALRFYGSDGV 240
           TKLKGITVKGTGTIDGQGS WWNDSPTYDPTDENLDKESSS KLPSTKPTALRFYGSDGV
Sbjct: 181 TKLKGITVKGTGTIDGQGSVWWNDSPTYDPTDENLDKESSSRKLPSTKPTALRFYGSDGV 240

Query: 241 TVTGITIQNSQQAHLKFDSCTSVQVSSLTVSSPGDSPNTDGIHLQNSQNVIISNSNLACG 300
           TV+GITIQNSQQAHLKFDSCTSVQVSSLT+SSPGDSPNTDGIHLQNSQNVIISNSNLACG
Sbjct: 241 TVSGITIQNSQQAHLKFDSCTSVQVSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLACG 300

Query: 301 DDCISIQTGSSAIYIHDVNCGPGHGISIGGLGRDQTKACVSNVTVRDTKLQNTMTGVRIK 360
           DDCISIQTGSSA+YIH+VNCGPGHGISIGGLGRDQTKACVSNVTVRD KLQNTM GVRIK
Sbjct: 301 DDCISIQTGSSAVYIHNVNCGPGHGISIGGLGRDQTKACVSNVTVRDVKLQNTMNGVRIK 360

Query: 361 TWQGGSGSVQGILFSNIQVSDVRTPIMIDQYYCDGGRCHNGSSAVAISGVNYVNIRGTYT 420
           TWQGGSG VQGILFSNIQVSDV+TPIMIDQYYCDGG CHNGSSAVAISGVNYVNIRGTYT
Sbjct: 361 TWQGGSGLVQGILFSNIQVSDVKTPIMIDQYYCDGGSCHNGSSAVAISGVNYVNIRGTYT 420

Query: 421 STPVHLACSDSLPCTGVTLDTIQLEGSGNSNEPFCWKAYGELKTSTVPPIDCLQSGNPSK 480
           STPVHLACSDSLPCTGVTLDTIQLEGSG SNEPFCWKAYGELKTSTVP I+CLQSGNP K
Sbjct: 421 STPVHLACSDSLPCTGVTLDTIQLEGSGRSNEPFCWKAYGELKTSTVPAIECLQSGNPGK 480

Query: 481 AQARDSC 483
           AQ+ +SC
Sbjct: 481 AQSHNSC 484

BLAST of Lcy09g019640 vs. NCBI nr
Match: KAG7017769.1 (Polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 865.5 bits (2235), Expect = 2.1e-247
Identity = 435/487 (89.32%), Postives = 444/487 (91.17%), Query Frame = 0

Query: 1   MEMGGKNLKCLATIAFICVFVWSFSLQNCHAREGRHWGAHRKTGGYRHRHAHRHRGGSGS 60
           MEMGGKNLKCLATIAFIC FVWSFSLQNC AREG+HW AH KTGGYRH   HR RGGSGS
Sbjct: 1   MEMGGKNLKCLATIAFICAFVWSFSLQNCQAREGKHW-AHGKTGGYRHGRIHRDRGGSGS 60

Query: 61  SRLMGSG-----DVVGASLASKGGASKGGDGSGNFNVLDFGAKGDGRTDDTKAFQAAWES 120
           SRL GSG     DVVGASLASKG ASKG  GSGNFNVLDFGAKGDGR DDTKAFQAAWES
Sbjct: 61  SRLTGSGHVGSEDVVGASLASKGDASKG--GSGNFNVLDFGAKGDGRADDTKAFQAAWES 120

Query: 121 ACNAEGSTVEVPSGSEFLVGPISFSGPNCQPNIVFQLDGKIIAPTSPSAWGSGLIQWIEF 180
           AC+AE STVEVPSGSEFLVGPISFSGPNC+PNIVFQLDGKIIAPTSPSAWGSGL+QWIEF
Sbjct: 121 ACSAEKSTVEVPSGSEFLVGPISFSGPNCKPNIVFQLDGKIIAPTSPSAWGSGLLQWIEF 180

Query: 181 TKLKGITVKGTGTIDGQGSAWWNDSPTYDPTDENLDKESSSGKLPSTKPTALRFYGSDGV 240
           TKLKGITVKGTGTIDGQGS WWNDSPTYDPTDENL               ALRFYGSDGV
Sbjct: 181 TKLKGITVKGTGTIDGQGSVWWNDSPTYDPTDENL---------------ALRFYGSDGV 240

Query: 241 TVTGITIQNSQQAHLKFDSCTSVQVSSLTVSSPGDSPNTDGIHLQNSQNVIISNSNLACG 300
           TV+GITIQNSQQAHLKFDSCTSVQVSSLT+SSPGDSPNTDGIHLQNSQNVIISNSNLACG
Sbjct: 241 TVSGITIQNSQQAHLKFDSCTSVQVSSLTISSPGDSPNTDGIHLQNSQNVIISNSNLACG 300

Query: 301 DDCISIQTGSSAIYIHDVNCGPGHGISIGGLGRDQTKACVSNVTVRDTKLQNTMTGVRIK 360
           DDCISIQTGSSA+YIH+VNCGPGHGISIGGLGRDQTKACVSNVTVRD KLQNTM GVRIK
Sbjct: 301 DDCISIQTGSSAVYIHNVNCGPGHGISIGGLGRDQTKACVSNVTVRDVKLQNTMNGVRIK 360

Query: 361 TWQGGSGSVQGILFSNIQVSDVRTPIMIDQYYCDGGRCHNGSSAVAISGVNYVNIRGTYT 420
           TWQGGSG VQGILFSNIQVSDV+TPIMIDQYYCDGG CHNGSSAVAISGVNYVNIRGTYT
Sbjct: 361 TWQGGSGLVQGILFSNIQVSDVKTPIMIDQYYCDGGSCHNGSSAVAISGVNYVNIRGTYT 420

Query: 421 STPVHLACSDSLPCTGVTLDTIQLEGSGNSNEPFCWKAYGELKTSTVPPIDCLQSGNPSK 480
           STPVHLACSDSLPCTGVTLDTIQLEGSG SNEPFCWKAYGELKTSTVP IDCLQSGNP K
Sbjct: 421 STPVHLACSDSLPCTGVTLDTIQLEGSGRSNEPFCWKAYGELKTSTVPAIDCLQSGNPGK 469

Query: 481 AQARDSC 483
           AQA DSC
Sbjct: 481 AQAHDSC 469

BLAST of Lcy09g019640 vs. TAIR 10
Match: AT5G14650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 553.1 bits (1424), Expect = 2.2e-157
Identity = 268/405 (66.17%), Postives = 314/405 (77.53%), Query Frame = 0

Query: 84  GDGSGNFNVLDFGAKGDGRTDDTKAFQAAWESACNAEGSTVEVPSGSEFLVGPISFSGPN 143
           G  SG  NVLD GAKGDG +DDTKAF+ AW+ AC    ST+ VPSGS FLVGP+SF G  
Sbjct: 45  GGSSGTINVLDHGAKGDGTSDDTKAFEDAWQVACKVAASTLLVPSGSTFLVGPVSFLGKE 104

Query: 144 CQPNIVFQLDGKIIAPTSPSAWGSGLIQWIEFTKLKGITVKGTGTIDGQGSAWWNDSPTY 203
           C+  IVFQL+GKIIAPTS SAWGSGL+QWIEF  L+GIT+KG G IDG+GS WWND    
Sbjct: 105 CKEKIVFQLEGKIIAPTSASAWGSGLLQWIEFKALQGITIKGKGIIDGRGSVWWND---- 164

Query: 204 DPTDENLDKESSSGKLPSTKPTALRFYGSDGVTVTGITIQNSQQAHLKFDSCTSVQVSSL 263
                         K+P TKPTALRFYGS+GVTV+GITIQNS Q HLKFD+C S+QVS  
Sbjct: 165 ----------MMGTKMPRTKPTALRFYGSNGVTVSGITIQNSPQTHLKFDNCISIQVSDF 224

Query: 264 TVSSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSAIYIHDVNCGPGHGISI 323
           T SSPGDSPNTDGIHLQNSQ+ +I  S LACGDDCISIQTG S I IHDV+CGPGHGISI
Sbjct: 225 TTSSPGDSPNTDGIHLQNSQDAVIYRSTLACGDDCISIQTGCSNINIHDVDCGPGHGISI 284

Query: 324 GGLGRDQTKACVSNVTVRDTKLQNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVRTPIMI 383
           GGLG+D TKACVSN+TVRD  +  T  GVRIK+WQGGSGSV+ ++FSNIQVS+V  PI+I
Sbjct: 285 GGLGKDNTKACVSNITVRDVTMHETTNGVRIKSWQGGSGSVKQVMFSNIQVSNVANPIII 344

Query: 384 DQYYCDGGRCHNGSSAVAISGVNYVNIRGTYTSTPVHLACSDSLPCTGVTLDTIQLE--- 443
           DQYYCDGG CHN +SAVA+S +NY+NI+GTYT  PV  ACSDSLPCTG++L TI+L+   
Sbjct: 345 DQYYCDGGGCHNETSAVAVSNINYINIKGTYTKEPVRFACSDSLPCTGISLSTIELKPAT 404

Query: 444 GSGNSNEPFCWKAYGELKTSTVPPIDCLQSGNPSKAQAR---DSC 483
           G  +S +PFCWKA+GELKT T+PPI CL++    +A +R   D+C
Sbjct: 405 GKASSLDPFCWKAHGELKTKTLPPIQCLKTEKSPEAASRSNNDAC 435

BLAST of Lcy09g019640 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 515.4 bits (1326), Expect = 5.0e-146
Identity = 271/523 (51.82%), Postives = 340/523 (65.01%), Query Frame = 0

Query: 8   LKCLATIAFICVFVWSFSLQNCHAREGRHWGAHRKTGG-----------YRHRHAHRHRG 67
           L+ +  +  + + VWS +L+ C AR GRHW  H ++               H + H H  
Sbjct: 9   LRSITMMTLVTILVWSATLETCIARRGRHWRHHHRSSSSLSDSLSSKKPKSHENNHHHMS 68

Query: 68  GSG------SSRLMGSGDVVGASLASKGGASKGG-----------------DGSGNFNVL 127
                     ++   S D  G+ + S+    +                   + S  FNVL
Sbjct: 69  HKSKPKPKMKTQPPKSND--GSPVVSQPPQVQQPPPPHVQPPPTPLPLQPVEDSQQFNVL 128

Query: 128 DFGAKGDGRTDDTKAFQAAWESACNAEGSTVEVPSGSEFLVGPISFSGPNCQPNIVFQLD 187
           DFGAKGDG +DDT+AF+AAW SAC  E ST+ +P    FLVGPISFSGP CQ NIVFQL+
Sbjct: 129 DFGAKGDGMSDDTQAFEAAWASACKVEASTMIIPPDYIFLVGPISFSGPYCQANIVFQLE 188

Query: 188 GKIIAPTSPSAWGSGLIQWIEFTKLKGITVKGTGTIDGQGSAWWNDSPTYD-PTDENLD- 247
           G I+APT   +WG GL+ WIEFTKL GIT++G G IDG+G+ WW      D P D++   
Sbjct: 189 GMIVAPTDTESWGGGLMWWIEFTKLSGITIQGNGVIDGRGTVWWQQDYLSDYPIDDDFKL 248

Query: 248 ----------------KESSSGKLPSTKPTALRFYGSDGVTVTGITIQNSQQAHLKFDSC 307
                           +   + ++PS KPTALRFYGS  VTVTGITIQNS Q HLKFD+C
Sbjct: 249 IVPLNNSVQERPPMPIRSELNWRMPSIKPTALRFYGSIDVTVTGITIQNSPQCHLKFDNC 308

Query: 308 TSVQVSSLTVSSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSAIYIHDVNC 367
             V V  + VSSPGDSPNTDGIHLQN+++V+I  + LACGDDCISIQTG S +Y+H+VNC
Sbjct: 309 VKVLVHDVNVSSPGDSPNTDGIHLQNTRDVMIHTTTLACGDDCISIQTGCSNVYVHNVNC 368

Query: 368 GPGHGISIGGLGRDQTKACVSNVTVRDTKLQNTMTGVRIKTWQGGSGSVQGILFSNIQVS 427
           GPGHGISIG LG+D TKACVSN+TVRD  + NTMTGVRIKTWQGG GSV+GILFSNIQ++
Sbjct: 369 GPGHGISIGSLGKDSTKACVSNITVRDVVMHNTMTGVRIKTWQGGIGSVKGILFSNIQLT 428

Query: 428 DVRTPIMIDQYYCDGGRCHNGSSAVAISGVNYVNIRGTYTSTPVHLACSDSLPCTGVTLD 476
           +V+ PI+IDQ+YCD  +C N +SAV++ GV Y  IRGTYT  PVH ACSDS PC  V L 
Sbjct: 429 EVQLPIVIDQFYCDHSKCMNHTSAVSVEGVTYEKIRGTYTVKPVHFACSDSFPCIDVQLS 488

BLAST of Lcy09g019640 vs. TAIR 10
Match: AT1G10640.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 505.0 bits (1299), Expect = 6.7e-143
Identity = 268/515 (52.04%), Postives = 335/515 (65.05%), Query Frame = 0

Query: 7   NLKCLATIAFICVFVWSFSLQNCHAREGRHW-GAHR--------------KTGGYRHRHA 66
           +L+ +  +  + V VWS +L+ C AR GRHW  +HR              K+ G  H H+
Sbjct: 8   SLRSITMMILMAVLVWSVTLETCIARRGRHWRHSHRSSSDLSDSLSNKKPKSHGNSHHHS 67

Query: 67  -----HRHRGGSGSSRLM--------GSGDVVGASLASKGGASKGGDGSGNFNVLDFGAK 126
                H H+        +         +  VV      +        GS  FNV+DFGAK
Sbjct: 68  SHNNNHHHKSKPKPKPKLKTPPKAHDNNSPVVSGPPKVQPPPLPPQKGSQVFNVMDFGAK 127

Query: 127 GDGRTDDTKAFQAAWESACNAEGSTVEVPSGSEFLVGPISFSGPNCQPNIVFQLDGKIIA 186
           GDG+ DDTKAF+AAW +AC  E S + VP    +LVGPISFSGP CQ NIVFQLDG IIA
Sbjct: 128 GDGKCDDTKAFEAAWVAACKIEASVMLVPPEYTYLVGPISFSGPYCQANIVFQLDGTIIA 187

Query: 187 PTSPSAWGSGLIQWIEFTKLKGITVKGTGTIDGQGSAWW-NDSPTYDPTDENLDKESSSG 246
           PT    WG GL+ WI+FTKLKGI V+G G IDG+GS WW  DSP  D   + +   ++S 
Sbjct: 188 PTDSKTWGKGLMWWIDFTKLKGIKVQGKGVIDGRGSGWWQQDSPFIDSDTKLIVPLNNSA 247

Query: 247 --------------KLPSTKPTALRFYGSDGVTVTGITIQNSQQAHLKFDSCTSVQVSSL 306
                         ++PS KPTALRF GS GV VTGITIQNS Q HLKFD C  V V  +
Sbjct: 248 NQNPPMPIRSELDERMPSIKPTALRFSGSFGVEVTGITIQNSPQCHLKFDDCVGVVVHDI 307

Query: 307 TVSSPGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSAIYIHDVNCGPGHGISI 366
            VSSPGDSPNTDGIHLQN+++V+I ++ LACGDDCISIQTG S +++H+VNCGPGHGISI
Sbjct: 308 AVSSPGDSPNTDGIHLQNTKDVLIHSTTLACGDDCISIQTGCSNVFVHNVNCGPGHGISI 367

Query: 367 GGLGRDQTKACVSNVTVRDTKLQNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVRTPIMI 426
           G LG++ TKACVSN+TVRD  + NTMTGVRIKTWQGG GSV+GI+FSNIQ++ V+ PI I
Sbjct: 368 GSLGKEGTKACVSNITVRDVAMHNTMTGVRIKTWQGGVGSVKGIIFSNIQLNQVQIPITI 427

Query: 427 DQYYCDGGRCHNGSSAVAISGVNYVNIRGTYTSTPVHLACSDSLPCTGVTLDTIQL---E 476
           +Q+YCD  +C N +SAVA+ GV Y  I+GTYT  PVH ACSD+ PC  V L +I+L   +
Sbjct: 428 NQFYCDHSKCKNQTSAVAVEGVTYERIKGTYTVKPVHFACSDNFPCVDVQLSSIELKPVQ 487

BLAST of Lcy09g019640 vs. TAIR 10
Match: AT3G26610.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 400.6 bits (1028), Expect = 1.8e-111
Identity = 198/396 (50.00%), Postives = 268/396 (67.68%), Query Frame = 0

Query: 88  GNFNVLDFGAKGDGRTDDTKAFQAAWESACNAEGSTVEVPSGSEFLVGPISFSGPNCQPN 147
           G FN+  +GAKGDG +DD+KA   AW++AC   G  VE+P+G++FLV  ++  GP C+  
Sbjct: 65  GVFNIFSYGAKGDGVSDDSKALVGAWKAACKVVGGKVEIPAGTQFLVKAVTLQGP-CKEE 124

Query: 148 IVFQLDGKIIAPTSPSAW-GSGLIQWIEFTKLKGITVKGTGTIDGQGSAWWNDSPTYDPT 207
            V Q++G ++AP    +W  S L QW+ F  +  +T++G+GT++G+G  WWN   TY   
Sbjct: 125 TVVQIEGILVAPEKIGSWPNSSLFQWLNFKWVSHLTIQGSGTLNGRGYNWWN-LDTYQTQ 184

Query: 208 DENLDKESSSGKLPSTKPTALRFYGSDGVTVTGITIQNSQQAHLKFDSCTSVQVSSLTVS 267
             N         +P  KPTALRFY S+ VTV  I+I NS   HLKFD    V+V+++T+S
Sbjct: 185 TRN-------KYIPPMKPTALRFYSSNNVTVRDISIVNSPLCHLKFDDSDGVKVNNITIS 244

Query: 268 SPGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSAIYIHDVNCGPGHGISIGGL 327
           SP +SPNTDGIHLQN++NV I +SN+ACGDDC+SIQTGSS ++IH +NCGPGHGISIGGL
Sbjct: 245 SPENSPNTDGIHLQNTRNVEIQHSNIACGDDCVSIQTGSSNVHIHHINCGPGHGISIGGL 304

Query: 328 GRDQTKACVSNVTVRDTKLQNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVRTPIMIDQY 387
           G+D++ ACVS++ V D  +QNT+ GVRIKTWQGG G V+ + FSNIQV DV+ PI+IDQY
Sbjct: 305 GKDKSVACVSDIIVEDISIQNTLAGVRIKTWQGGLGVVKNLTFSNIQVKDVKVPIVIDQY 364

Query: 388 YCDGGRCHNGSSAVAISGVNYVNIRGTYTSTPVHLACSDSLPCTGVTLDTIQLEGSG--- 447
           YCD  +C N + AV+ISGV Y NI G++T  PV +ACS+++PC  V L  I+L  SG   
Sbjct: 365 YCDKSKCKNQTRAVSISGVKYNNIVGSFTVQPVRIACSNNVPCMDVDLMDIRLRPSGGIR 424

Query: 448 -----NSNEPFCWKAYGELKTSTVPPI--DCLQSGN 473
                   +  CW +YG+ +   VP     CL+  N
Sbjct: 425 GLQTHQQQQALCWNSYGKTQGPLVPSSIGYCLRKSN 451

BLAST of Lcy09g019640 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 376.3 bits (965), Expect = 3.6e-104
Identity = 189/398 (47.49%), Postives = 253/398 (63.57%), Query Frame = 0

Query: 90  FNVLDFGAKGDGRTDDTKAFQAAWESACNAEGSTVEVPSGSEFLVGPISFSGPNCQPNIV 149
           F+V  FGA GDG  DDT AFQ AW++AC  E   V  P G  F +    FSGP C+P +V
Sbjct: 80  FDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLAPEGGVFKITSTIFSGP-CKPGLV 139

Query: 150 FQLDGKIIAPTSPSAW--GSGLIQWIEFTKLKGITVKGTGTIDGQGSAWWNDSPTYDPTD 209
           FQLDG ++ P  P  W       QW+ F +L G T  G GT++G G  WW+      P  
Sbjct: 140 FQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWD-----LPCK 199

Query: 210 ENLDKESSSGKLPSTKPTALRFYGSDGVTVTGITIQNSQQAHLKFDSCTSVQVSSLTVSS 269
            +   + SS   P   PT +RF+ S+ + V G+ IQNS Q H+KFD C  V ++ + +SS
Sbjct: 200 PHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISS 259

Query: 270 PGDSPNTDGIHLQNSQNVIISNSNLACGDDCISIQTGSSAIYIHDVNCGPGHGISIGGLG 329
           P  SPNTDGIHL N+++V I NS ++ GDDCISI TG S + I  V CGP HGISIG LG
Sbjct: 260 PKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGISIGSLG 319

Query: 330 RDQTKACVSNVTVRDTKLQNTMTGVRIKTWQGGSGSVQGILFSNIQVSDVRTPIMIDQYY 389
              ++ACVSN+TVR+T ++++  G+R+KTWQGG+GSV  +LF NIQ+ +V   I++DQYY
Sbjct: 320 VHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNCIIVDQYY 379

Query: 390 CDGGRCHNGSSAVAISGVNYVNIRGTY--TSTPVHLACSDSLPCTGVTLDTIQL--EGSG 449
           C    C N +SAV +  V Y NI+GTY   S P+H ACSD++ CT +T+  ++L  E   
Sbjct: 380 CQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLPEEGE 439

Query: 450 NSNEPFCWKAYGELKTSTVPPIDCLQSGNPSKAQARDS 482
             ++PFCW AYG+ +T T+PPIDCL  G+P   +A DS
Sbjct: 440 LVDDPFCWNAYGKQETLTIPPIDCLLDGSPVVEEAYDS 471

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949Z15.1e-10347.49Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV... [more]
Q94AJ53.2e-8142.89Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
P353369.3e-8143.07Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
Q8RY291.3e-7944.66Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2[more]
P432123.7e-7742.18Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F9Z95.2e-26092.61polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111442159 P... [more]
A0A6J1J1T61.7e-25591.17polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111481937 PE=... [more]
A0A6J1EJN21.9e-24688.82polygalacturonase At1g48100 OS=Cucurbita moschata OX=3662 GN=LOC111435099 PE=3 S... [more]
A0A6J1JNK81.2e-24588.20polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111486313 PE=3 SV=... [more]
A0A1S3B8B86.4e-24286.68polygalacturonase At1g48100 OS=Cucumis melo OX=3656 GN=LOC103487102 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_023527919.11.1e-25992.61polygalacturonase At1g48100-like [Cucurbita pepo subsp. pepo][more]
XP_022935210.11.1e-25992.61polygalacturonase At1g48100-like [Cucurbita moschata][more]
XP_038905486.13.5e-25890.55polygalacturonase At1g48100 [Benincasa hispida][more]
XP_022983321.13.6e-25591.17polygalacturonase At1g48100-like [Cucurbita maxima][more]
KAG7017769.12.1e-24789.32Polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT5G14650.12.2e-15766.17Pectin lyase-like superfamily protein [more]
AT1G60590.15.0e-14651.82Pectin lyase-like superfamily protein [more]
AT1G10640.16.7e-14352.04Pectin lyase-like superfamily protein [more]
AT3G26610.11.8e-11150.00Pectin lyase-like superfamily protein [more]
AT1G48100.13.6e-10447.49Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 282..303
e-value: 190.0
score: 10.8
coord: 232..281
e-value: 11.0
score: 14.9
coord: 335..356
e-value: 36.0
score: 13.2
coord: 364..385
e-value: 1800.0
score: 5.4
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 80..478
e-value: 7.0E-124
score: 415.9
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 122..445
e-value: 3.3E-85
score: 286.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 40..65
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 191..223
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 40..57
NoneNo IPR availablePANTHERPTHR31375:SF171PECTIN LYASE-LIKE SUPERFAMILY PROTEINcoord: 10..473
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 10..473
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 90..472

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g019640.1Lcy09g019640.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0009827 plant-type cell wall modification
cellular_component GO:0016020 membrane
cellular_component GO:0009505 plant-type cell wall
molecular_function GO:0047911 galacturan 1,4-alpha-galacturonidase activity
molecular_function GO:0004650 polygalacturonase activity