Lcy09g019460 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g019460
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionFormate dehydrogenase, mitochondrial
LocationChr09: 43987044 .. 43992123 (-)
RNA-Seq ExpressionLcy09g019460
SyntenyLcy09g019460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTACAAGAAAGGCAGCCAGCGCCCAGCGGTAGCACAGAGAAACATAACTGAGACAGAAACAAAAGCAAAGAGCGCCTTCGCTTCCAATCCCGTTTCGCCAACTATAAATACACACCCACACACAAGCCAACACAAACTCCACATTCATCATCATCATTCATTCATTCATTCACTCACGAGTCTCTCTCTCGCACACACTCATACCTAACTCTCTCACAGATTCTTCAGAGAACAAGACGACAATAATGGCGATGAAGCAGGCTGCTAAAGCGGCGATCCGAGCTCTTTCCTCCTCTGTATCCTCTCAGTCATCACCTCTCGTCAGGCATCTTCATGTAAGTCATTTGACCTTTTCTTCTTCAGCCAATTTCATTTGTCCAATTAGTGTGTTGAGGTTCTTCTGGTTCTCCTGCGGAGTTTCTGAAGTTAGGAAGTTGAATATTCTGTGAATTTTTTTCTTCCGATGGCTTCGATCGCTGAAATGTGGAATGTTCGTCTCTCGTTCTTCGATTCTGTTTTTCTTCCCCTATTTTAGACTGTTTGTGTAGTTTTTTCTAGTCTCCGATCGTTATTTGCTAGAATAGGAAGCCTGGTTTGTGTCTCTGAGTGATTGTGCGTTTGATTGGGAGCTGGAGGGAGGGTTTATGGATAATCGAAGTTCCTTTAGTGATTTGTAAGCTTTTTGAGTCTCAGATCGTGAAGATTACGAGAACAGGAAGCTCAGTTTGTGCCTCTGTGTGACTATGGGCTTGATTGGGAGGTGGAGGAAGGGTTTATGGATAATTGAAATTGCTTTACTGATTTGTTAGTTTGTTTTTGTTTCTGATCGTGAAGATAGTTTGCTAGAACAGGAAACCTAGTTTGTGTCTCTGTGCGATTATGAGCTTGATTGGGAGGTGGAGGAAGGGTTTATGGATGATTGAAGTTGATTTCGTGATTTGTGAATCTGAAGTTAGTTAATCAATGGCTTTCGTTTGTCTCTTGTTTTGAGCAGGCTTCCGCAGGGAGCAAGAAGATCGTCGGTGTGTTTTACAAGGCTAATGAGTATGCTGCTAAGAACCCTGATTTTGTGGGATGTGCGGAGGGAGCCTTGGGCATACGCGAATGGTTGGAATCTCAAGGGCATCAGTATATTGTCACTGATGACAAAGAAGGACTCGATTGTGGTAAGCATCTGACTAAATATTCTACTTGATCCGCCTGTCTGTATTCCCTATTAAACTAAGGGCGATTTTTGTTCCCGCTAAGCTCGGACAGATTAGGATGACGAAGAACTTTCGGGGCCTGGCAGAGAACTAAAAATGCCCATTTTAGGCTGAAATAAAATAAAACATACATACTTAAATATTTTAAATATTAAAATTTGATTGGGCTCACTGTTTTAAAATTAATTTAAAAGATAATATTTTTAATTTGAACTTTTCATTTCAACTTTTGAAGTCAATATCTTAGATATGTTATTTCATATTGATGTTTTTTTTTAATAAAAACATCAATAAATGATTACTAATTTTTTTTTTAAAAAAAAAATGATTACTAATTAGACTTTGTCACTCAACTATTTAATTTCATATTTTATAATTCAAGTGTAAAACTATTTAAAAGAAGGGTCAAGCATATTTTTCAAATAAAACCAACAAAAACTTTATGGACTAAAAGTATCTAATTTTGTTAGTGAAAATGCTAGACAGTTAATATAGGTGCATGATATATATTTCTTCTTAATACGAAAATCTTAACATTAGGTCAAAAGTCAAAATGGAAAAATTTACAATTCTGTACACATATATAGAAAGTGACGTTTAACTACTAAATATTATCAAGAAGTAATAAATATATTTTATTACTTAAAAAAAAACCAATATATTTTATTCTGTACACATATTTTGAGATAAATATATTTTTATCTCAAAAAAGAAAAAAAGTAATAAATATATTTTTTAATTTTAAAGAAAAAGATTTAAAAAGGACTACAAAGAATTATTAAGTTCATGTTTAATAACTACTCCGTTTTTTGTTTTTTTTTTAAAAAATTTATGCCTACTTCACATCAATTTCTATAGTATGTTTTCACTGTTTTTGACAAACATTTAAATTCATAGTCAAATTTCAAAGTGCAAGTAAAAGATAGAGGCTGATTTTGAAGTTTGGTTTTAAACAAAATTTGGTTTAGATTTTGAAAACATTTTTAAAAAGCAGAAGACATAGTAAAGAAATACATAAGTTTTTTCTTTTTTTTTTTTGGATAAAAAGTCCAAGTAGTGTTAATAAACTAAAATTTCAAAAAGTAGAAAACCAAAACTAAAATGGTTATCAAGTGTGCTTGACCATGTTATATGATATCAAATTAGCTTAAGAACATAAAAGATATTTTCAATTCCTACAAATGTATACAGAAGGTGATTAATAGTTACATATTATCTTTGTTCTGATTGAAGTCATGTGATTTTGTGCATCCTTATTTCAGAGCTTGAAAAGCATATTCCAGATCTCCATGTTCTCATAACAACCCCCTTCCATCCTGCGTATGTAACTGCTGAAAGGATCAAAAAGGCCAAAAACTTGCAGTTGCTTCTCACGGCTGGAATTGGATCTGATCATATTGACCTCAATGCTGCTGCTGCTGCTGGACTAACAGTTGCAGAGGTTACCGGAAGCAATGTGGTTTCAGTTGCAGAGGATGAGCTAATGAGAATTCTCATTCTCGTCAGGAATTTCCTACCAGGACACCACCAGGTTATTCATGGCGACTGGAATGTTGCTGGAATAGCTTACAGAGCTTATGATCTTGAGGGGAAGACGGTTGGAACCGTAGGTGCTGGACGGATCGGCAAACTCTTACTTCAAAGGCTGAAGCCTTTCAATTGCAATCTCCTCTACCACGACCGACTCAAGATTGATCCTGAACTCGAGAAGGAAATCGGAGCGCAGTTTGAGGAGGATCTGGATGCAATGCTACCAAAATGTGATGTACTTGTCATCAACACTCCTCTGACTGACAAGACAAGGTATTGATTTTACTTTGGATTATAAATTGAGATGATGAACATAAGGAAAATAGTGAATACTACTTTAGATTGGAAGCTTTGGATATTTGCCACTCATGTCCGAAAACAACTAATGGAAACTTTGACACTACTTCTCTGCAGGGGACTATTTAACAAAGAGAGAATTGCCAAGTGCAAGAAAGGAGTACTGATTGTTAACAATGCTCGAGGAGCAATCATGGACACTCAAGCAGTTGTTGACGCTTGTAACAGTGGACACATTGGAGGTAATTGTAGAACATAATTCTATAGCAATGCGGCAGCATAATCTGATAAGTCCGACATGATGCCTTGATATGTCATATACATTACATATAAATAGGAAGTCGAAGTTATATATCAAGGTGCTACATTAACTTTGTTGACACATTTATTAACTCTTTGGTGCTATATAATTTGATAATTGATATTTGATGTTAGGAGTGGATATGTATGCAAAATTTTAGTAACTAATGAAACAAAAGAAATATATATAGAGAGAGAGAGACTGTAATTGTAACCATTGAAAACAAGAATCATGTCAAATTTAAATCCATAATCTAAGAGGCTCTTGTTGATCTCCCCCCAAAAGCTTATCTTATGACTTGCTATGGATCATTTAGGGAAATGAAGAAGCTCATCCCAATTCCATCATTGGCTAATACTTCAAAGTATTCTGAATATGCAGGTTACAGTGGTGATGTGTGGAACCCACAACCAGCTCCAAAAGACCATCCATGGCGTTACATGCCAAACCAAGCTATGACACCTCATATTTCTGGCACCACCATTGATGGACAGGTACGGTATAGTTGCTTTGAAATTATTTTCTCCTCACTCTCTTCAAGAACGAAGTAGAGATTTTAAAGTATAACCTTTAGTCTTACCCCCTCCAATTTTAGGAAAATTATAAGTTTCGCTTCTAAACTTTTAGGGTTGAGTCTATTTAGTCTCCGTAGTATAAAAAGTTTCAAACAAGTTCCTAAAATTTTAATTTTGCGTCTAATAAATCTCCTACTCTACTGCTGACTCCATAAACTTAATGTTTATGGCTCTTAAGTGCTTGAATCTTTCTAAAATCAACCTATTGTGCAATCTTTAAATAATATAGGTAACATGTTATCTAAGTGCTAAATTGACAAAATGAATGACAGTAACCTATTAGACATAAAATTGAAAGTTCAAAGACTTATTAGAAACTTTTTAACGTACAAGGACAAAAATGGACACGATTGTGAAATTCTGGGGTCTATTAGAAAATTTTAAGTAAACATAATCTTGAAAAGTTTATGGACCAAATTTGCAATTTAAATTTCTAAACCTAAAAGTTAGAATGTAAAATTTTGTCGGGTATATCTATGTGTATGTCATCGACCACTCTTATCCGATGCCTTTCGGTTTGCTCGATTTCTCTGTTGCCTTTTATTAACTAGTATACCTTATGGAAACAATGCAGTTGCGATATGCTGCGGGAGTGAAGGACATGCTTGAAAGGTACTTCAAAGGAGAAGAATTTCCTGCTCAAAACTACATCGTCAAGGAAGGAGAACTTGCACCTCAGTACCGATAAGTATTTCTCTGTATGGATTTGCTATCTCTCCCTTTTAGTCAATAATTAGTTCCCAAAATAAAATGACTGAATGGGAGTTAACTTATGGCTTTAAAATCAGTGTCTTGTGAGTCTTTTCTGTAATGAGAATGAATGCATCTCATCCATAATGTAATTCCATGTAAAATGGATGTATGAGGATGAATGAGAGTTTCTTTCCTTTCCCTCTAATTGATCATGAAATTGCAAGGAAAAAAAAATCTAAACTAATGAAATTGACCAAAATCAAACTGAATTGTTAGGAAGGGACCATGTAAATTGAATTGATCAACGATGATAGTTCAAAGCGACAAATGGGACATTTTCGGTATTGTTGATAGTTTTAGTTTCAACGTGCATCCCTACTCCAAGTAGATATGTGAACTATTAGTACTAGTGTAGATTATTTTTTTTTAGTATCTGTGAGTGTATAAGTCAATTTGTACGCACCTTGACTATTCTCACG

mRNA sequence

GTACAAGAAAGGCAGCCAGCGCCCAGCGGTAGCACAGAGAAACATAACTGAGACAGAAACAAAAGCAAAGAGCGCCTTCGCTTCCAATCCCGTTTCGCCAACTATAAATACACACCCACACACAAGCCAACACAAACTCCACATTCATCATCATCATTCATTCATTCATTCACTCACGAGTCTCTCTCTCGCACACACTCATACCTAACTCTCTCACAGATTCTTCAGAGAACAAGACGACAATAATGGCGATGAAGCAGGCTGCTAAAGCGGCGATCCGAGCTCTTTCCTCCTCTGTATCCTCTCAGTCATCACCTCTCGTCAGGCATCTTCATGCTTCCGCAGGGAGCAAGAAGATCGTCGGTGTGTTTTACAAGGCTAATGAGTATGCTGCTAAGAACCCTGATTTTGTGGGATGTGCGGAGGGAGCCTTGGGCATACGCGAATGGTTGGAATCTCAAGGGCATCAGTATATTGTCACTGATGACAAAGAAGGACTCGATTGTGAGCTTGAAAAGCATATTCCAGATCTCCATGTTCTCATAACAACCCCCTTCCATCCTGCGTATGTAACTGCTGAAAGGATCAAAAAGGCCAAAAACTTGCAGTTGCTTCTCACGGCTGGAATTGGATCTGATCATATTGACCTCAATGCTGCTGCTGCTGCTGGACTAACAGTTGCAGAGGTTACCGGAAGCAATGTGGTTTCAGTTGCAGAGGATGAGCTAATGAGAATTCTCATTCTCGTCAGGAATTTCCTACCAGGACACCACCAGGTTATTCATGGCGACTGGAATGTTGCTGGAATAGCTTACAGAGCTTATGATCTTGAGGGGAAGACGGTTGGAACCGTAGGTGCTGGACGGATCGGCAAACTCTTACTTCAAAGGCTGAAGCCTTTCAATTGCAATCTCCTCTACCACGACCGACTCAAGATTGATCCTGAACTCGAGAAGGAAATCGGAGCGCAGTTTGAGGAGGATCTGGATGCAATGCTACCAAAATGTGATGTACTTGTCATCAACACTCCTCTGACTGACAAGACAAGGGGACTATTTAACAAAGAGAGAATTGCCAAGTGCAAGAAAGGAGTACTGATTGTTAACAATGCTCGAGGAGCAATCATGGACACTCAAGCAGTTGTTGACGCTTGTAACAGTGGACACATTGGAGGTTACAGTGGTGATGTGTGGAACCCACAACCAGCTCCAAAAGACCATCCATGGCGTTACATGCCAAACCAAGCTATGACACCTCATATTTCTGGCACCACCATTGATGGACAGTTGCGATATGCTGCGGGAGTGAAGGACATGCTTGAAAGGTACTTCAAAGGAGAAGAATTTCCTGCTCAAAACTACATCGTCAAGGAAGGAGAACTTGCACCTCAGTACCGATAAGTATTTCTCTGTATGGATTTGCTATCTCTCCCTTTTAGTCAATAATTAGTTCCCAAAATAAAATGACTGAATGGGAGTTAACTTATGGCTTTAAAATCAGTGTCTTGTGAGTCTTTTCTGTAATGAGAATGAATGCATCTCATCCATAATGTAATTCCATGTAAAATGGATGTATGAGGATGAATGAGAGTTTCTTTCCTTTCCCTCTAATTGATCATGAAATTGCAAGGAAAAAAAAATCTAAACTAATGAAATTGACCAAAATCAAACTGAATTGTTAGGAAGGGACCATGTAAATTGAATTGATCAACGATGATAGTTCAAAGCGACAAATGGGACATTTTCGGTATTGTTGATAGTTTTAGTTTCAACGTGCATCCCTACTCCAAGTAGATATGTGAACTATTAGTACTAGTGTAGATTATTTTTTTTTAGTATCTGTGAGTGTATAAGTCAATTTGTACGCACCTTGACTATTCTCACG

Coding sequence (CDS)

ATGGCGATGAAGCAGGCTGCTAAAGCGGCGATCCGAGCTCTTTCCTCCTCTGTATCCTCTCAGTCATCACCTCTCGTCAGGCATCTTCATGCTTCCGCAGGGAGCAAGAAGATCGTCGGTGTGTTTTACAAGGCTAATGAGTATGCTGCTAAGAACCCTGATTTTGTGGGATGTGCGGAGGGAGCCTTGGGCATACGCGAATGGTTGGAATCTCAAGGGCATCAGTATATTGTCACTGATGACAAAGAAGGACTCGATTGTGAGCTTGAAAAGCATATTCCAGATCTCCATGTTCTCATAACAACCCCCTTCCATCCTGCGTATGTAACTGCTGAAAGGATCAAAAAGGCCAAAAACTTGCAGTTGCTTCTCACGGCTGGAATTGGATCTGATCATATTGACCTCAATGCTGCTGCTGCTGCTGGACTAACAGTTGCAGAGGTTACCGGAAGCAATGTGGTTTCAGTTGCAGAGGATGAGCTAATGAGAATTCTCATTCTCGTCAGGAATTTCCTACCAGGACACCACCAGGTTATTCATGGCGACTGGAATGTTGCTGGAATAGCTTACAGAGCTTATGATCTTGAGGGGAAGACGGTTGGAACCGTAGGTGCTGGACGGATCGGCAAACTCTTACTTCAAAGGCTGAAGCCTTTCAATTGCAATCTCCTCTACCACGACCGACTCAAGATTGATCCTGAACTCGAGAAGGAAATCGGAGCGCAGTTTGAGGAGGATCTGGATGCAATGCTACCAAAATGTGATGTACTTGTCATCAACACTCCTCTGACTGACAAGACAAGGGGACTATTTAACAAAGAGAGAATTGCCAAGTGCAAGAAAGGAGTACTGATTGTTAACAATGCTCGAGGAGCAATCATGGACACTCAAGCAGTTGTTGACGCTTGTAACAGTGGACACATTGGAGGTTACAGTGGTGATGTGTGGAACCCACAACCAGCTCCAAAAGACCATCCATGGCGTTACATGCCAAACCAAGCTATGACACCTCATATTTCTGGCACCACCATTGATGGACAGTTGCGATATGCTGCGGGAGTGAAGGACATGCTTGAAAGGTACTTCAAAGGAGAAGAATTTCCTGCTCAAAACTACATCGTCAAGGAAGGAGAACTTGCACCTCAGTACCGATAA

Protein sequence

MAMKQAAKAAIRALSSSVSSQSSPLVRHLHASAGSKKIVGVFYKANEYAAKNPDFVGCAEGALGIREWLESQGHQYIVTDDKEGLDCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNLQLLLTAGIGSDHIDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIHGDWNVAGIAYRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKEIGAQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVVDACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDGQLRYAAGVKDMLERYFKGEEFPAQNYIVKEGELAPQYR
Homology
BLAST of Lcy09g019460 vs. ExPASy Swiss-Prot
Match: Q07511 (Formate dehydrogenase, mitochondrial OS=Solanum tuberosum OX=4113 GN=FDH1 PE=1 SV=2)

HSP 1 Score: 662.1 bits (1707), Expect = 3.7e-189
Identity = 324/384 (84.38%), Postives = 349/384 (90.89%), Query Frame = 0

Query: 1   MAMKQAAKAAIRALSSSVSSQSSPLVRHLHASAGSKKIVGVFYKANEYAAKNPDFVGCAE 60
           MAM + A  A RA++   S  S    R L AS G KKIVGVFYKANEYA  NP+F+GCAE
Sbjct: 1   MAMSRVASTAARAIT---SPSSLVFTRELQASPGPKKIVGVFYKANEYAEMNPNFLGCAE 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120
            ALGIREWLES+GHQYIVT DKEG DCELEKHIPDLHVLI+TPFHPAYVTAERIKKAKNL
Sbjct: 61  NALGIREWLESKGHQYIVTPDKEGPDCELEKHIPDLHVLISTPFHPAYVTAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHIDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIH 180
           QLLLTAGIGSDH+DL AAAAAGLTVAEVTGSN VSVAEDELMRILILVRNFLPGHHQVI+
Sbjct: 121 QLLLTAGIGSDHVDLKAAAAAGLTVAEVTGSNTVSVAEDELMRILILVRNFLPGHHQVIN 180

Query: 181 GDWNVAGIAYRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKEIG 240
           G+WNVA IA+RAYDLEGKTVGTVGAGRIG+LLLQRLKPFNCNLLYHDRLK+D ELE +IG
Sbjct: 181 GEWNVAAIAHRAYDLEGKTVGTVGAGRIGRLLLQRLKPFNCNLLYHDRLKMDSELENQIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           A+FEEDLD ML KCD++VINTPLT+KT+G+F+KERIAK KKGVLIVNNARGAIMDTQAVV
Sbjct: 241 AKFEEDLDKMLSKCDIVVINTPLTEKTKGMFDKERIAKLKKGVLIVNNARGAIMDTQAVV 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDGQLRYAAGVKDMLER 360
           DACNSGHI GYSGDVW PQPAPKDHPWRYMPNQAMTPHISGTTID QLRYAAG KDML+R
Sbjct: 301 DACNSGHIAGYSGDVWYPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGTKDMLDR 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 385
           YFKGE+FPA+NYIVK+GELAPQYR
Sbjct: 361 YFKGEDFPAENYIVKDGELAPQYR 381

BLAST of Lcy09g019460 vs. ExPASy Swiss-Prot
Match: Q9S7E4 (Formate dehydrogenase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=FDH1 PE=1 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 1.3e-186
Identity = 324/385 (84.16%), Postives = 351/385 (91.17%), Query Frame = 0

Query: 1   MAMKQAAKAAIRALSSSVSSQSSPLVRHLHASAG-SKKIVGVFYKANEYAAKNPDFVGCA 60
           MAM+QAAKA IRA SSS SS      R  +AS+G SKKIVGVFYKANEYA KNP+F+GC 
Sbjct: 1   MAMRQAAKATIRACSSS-SSSGYFARRQFNASSGDSKKIVGVFYKANEYATKNPNFLGCV 60

Query: 61  EGALGIREWLESQGHQYIVTDDKEGLDCELEKHIPDLHVLITTPFHPAYVTAERIKKAKN 120
           E ALGIR+WLESQGHQYIVTDDKEG DCELEKHIPDLHVLI+TPFHPAYVTAERIKKAKN
Sbjct: 61  ENALGIRDWLESQGHQYIVTDDKEGPDCELEKHIPDLHVLISTPFHPAYVTAERIKKAKN 120

Query: 121 LQLLLTAGIGSDHIDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVI 180
           L+LLLTAGIGSDHIDL AAAAAGLTVAEVTGSNVVSVAEDELMRILIL+RNF+PG++QV+
Sbjct: 121 LKLLLTAGIGSDHIDLQAAAAAGLTVAEVTGSNVVSVAEDELMRILILMRNFVPGYNQVV 180

Query: 181 HGDWNVAGIAYRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKEI 240
            G+WNVAGIAYRAYDLEGKT+GTVGAGRIGKLLLQRLKPF CNLLYHDRL++ PELEKE 
Sbjct: 181 KGEWNVAGIAYRAYDLEGKTIGTVGAGRIGKLLLQRLKPFGCNLLYHDRLQMAPELEKET 240

Query: 241 GAQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAV 300
           GA+F EDL+ MLPKCDV+VIN PLT+KTRG+FNKE I K KKGVLIVNNARGAIM+ QAV
Sbjct: 241 GAKFVEDLNEMLPKCDVIVINMPLTEKTRGMFNKELIGKLKKGVLIVNNARGAIMERQAV 300

Query: 301 VDACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDGQLRYAAGVKDMLE 360
           VDA  SGHIGGYSGDVW+PQPAPKDHPWRYMPNQAMTPH SGTTID QLRYAAG KDMLE
Sbjct: 301 VDAVESGHIGGYSGDVWDPQPAPKDHPWRYMPNQAMTPHTSGTTIDAQLRYAAGTKDMLE 360

Query: 361 RYFKGEEFPAQNYIVKEGELAPQYR 385
           RYFKGE+FP +NYIVK+GELAPQYR
Sbjct: 361 RYFKGEDFPTENYIVKDGELAPQYR 384

BLAST of Lcy09g019460 vs. ExPASy Swiss-Prot
Match: Q9SXP2 (Formate dehydrogenase 1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0486800 PE=1 SV=2)

HSP 1 Score: 649.4 bits (1674), Expect = 2.5e-185
Identity = 321/386 (83.16%), Postives = 346/386 (89.64%), Query Frame = 0

Query: 1   MAMKQAAKAAI--RALSSSVSSQSSPLVRHLHASAGSKKIVGVFYKANEYAAKNPDFVGC 60
           MAM +AA   +  RAL S          R  H SAGSKKIVGVFYK  EYA KNP+FVGC
Sbjct: 1   MAMWRAAAGHLLGRALGS----------RAAHTSAGSKKIVGVFYKGGEYADKNPNFVGC 60

Query: 61  AEGALGIREWLESQGHQYIVTDDKEGLDCELEKHIPDLHVLITTPFHPAYVTAERIKKAK 120
            EGALGIREWLES+GH YIVTDDKEGL+ ELEKHI D+HVLITTPFHPAYV+AERIKKAK
Sbjct: 61  VEGALGIREWLESKGHHYIVTDDKEGLNSELEKHIEDMHVLITTPFHPAYVSAERIKKAK 120

Query: 121 NLQLLLTAGIGSDHIDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQV 180
           NL+LLLTAGIGSDHIDL AAAAAGLTVAEVTGSN VSVAEDELMRILIL+RNFLPG+ QV
Sbjct: 121 NLELLLTAGIGSDHIDLPAAAAAGLTVAEVTGSNTVSVAEDELMRILILLRNFLPGYQQV 180

Query: 181 IHGDWNVAGIAYRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKE 240
           +HG+WNVAGIAYRAYDLEGKTVGTVGAGRIG+LLLQRLKPFNCNLLYHDRLKIDPELEKE
Sbjct: 181 VHGEWNVAGIAYRAYDLEGKTVGTVGAGRIGRLLLQRLKPFNCNLLYHDRLKIDPELEKE 240

Query: 241 IGAQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQA 300
           IGA++EEDLDAMLPKCDV+VINTPLT+KTRG+FNKERIAK KKGV+IVNNARGAIMDTQA
Sbjct: 241 IGAKYEEDLDAMLPKCDVIVINTPLTEKTRGMFNKERIAKMKKGVIIVNNARGAIMDTQA 300

Query: 301 VVDACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDGQLRYAAGVKDML 360
           V DAC+SG + GY GDVW PQPAPKDHPWRYMPN AMTPHISGTTID QLRYAAGVKDML
Sbjct: 301 VADACSSGQVAGYGGDVWFPQPAPKDHPWRYMPNHAMTPHISGTTIDAQLRYAAGVKDML 360

Query: 361 ERYFKGEEFPAQNYIVKEGELAPQYR 385
           +RYFKGE+FP QNYIVKEG+LA QY+
Sbjct: 361 DRYFKGEDFPVQNYIVKEGQLASQYQ 376

BLAST of Lcy09g019460 vs. ExPASy Swiss-Prot
Match: Q67U69 (Formate dehydrogenase 2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0486900 PE=2 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 7.5e-182
Identity = 306/384 (79.69%), Postives = 345/384 (89.84%), Query Frame = 0

Query: 1   MAMKQAAKAAIRALSSSVSSQSSPLVRHLHASAGSKKIVGVFYKANEYAAKNPDFVGCAE 60
           MAM +A  AA + L  +++S ++        SAGSKK+VGVFYK  EYA KNP+FVGC +
Sbjct: 1   MAMWRAPSAAGQLLGRALASTAA------QTSAGSKKVVGVFYKGGEYADKNPNFVGCVD 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120
            ALGIR WLES+GH+YIVTDDKEG++CELEKHI D HVLITTPFHPAY+TAERIKKAKNL
Sbjct: 61  SALGIRGWLESKGHRYIVTDDKEGINCELEKHIEDAHVLITTPFHPAYITAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHIDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIH 180
           +LLLTAG+GSDHIDL AAAAAGLTVAE+TGSN VSVAED+LMRIL+L+RNFLPGHHQ+++
Sbjct: 121 ELLLTAGVGSDHIDLPAAAAAGLTVAEITGSNTVSVAEDQLMRILLLLRNFLPGHHQIVN 180

Query: 181 GDWNVAGIAYRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKEIG 240
           G+WNVAGIA+R YDLEGKTVGTVGAGRIG+LLLQRLKPFNCNL+YHDR+KIDPELEKEIG
Sbjct: 181 GEWNVAGIAHRTYDLEGKTVGTVGAGRIGRLLLQRLKPFNCNLMYHDRVKIDPELEKEIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           A++EEDLDAMLPKCDV+VIN PLT+KTRG+FNKERIAK KKGV IVNNARGAIMDTQAV 
Sbjct: 241 AKYEEDLDAMLPKCDVVVINMPLTEKTRGMFNKERIAKMKKGVTIVNNARGAIMDTQAVA 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDGQLRYAAGVKDMLER 360
           DAC SGH+ GY GDVW PQPAPKDHPWRYMPN AMTPH SGTTIDGQLRYAAGVKDML+R
Sbjct: 301 DACASGHVAGYGGDVWFPQPAPKDHPWRYMPNHAMTPHCSGTTIDGQLRYAAGVKDMLDR 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 385
           YFKGE+FPAQNYIVK G+LA QY+
Sbjct: 361 YFKGEDFPAQNYIVKAGQLASQYQ 378

BLAST of Lcy09g019460 vs. ExPASy Swiss-Prot
Match: Q9ZRI8 (Formate dehydrogenase, mitochondrial OS=Hordeum vulgare OX=4513 PE=2 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 1.3e-181
Identity = 305/358 (85.20%), Postives = 331/358 (92.46%), Query Frame = 0

Query: 27  RHLHASAGSKKIVGVFYKANEYAAKNPDFVGCAEGALGIREWLESQGHQYIVTDDKEGLD 86
           R  H SAGSKKIVGVFY+A EYA KNP+FVGC EGALGIR+WLES+GH YIVTDDKEG +
Sbjct: 20  RAAHTSAGSKKIVGVFYQAGEYADKNPNFVGCVEGALGIRDWLESKGHHYIVTDDKEGFN 79

Query: 87  CELEKHIPDLHVLITTPFHPAYVTAERIKKAKNLQLLLTAGIGSDHIDLNAAAAAGLTVA 146
            ELEKHI D+HVLITTPFHPAYVTAE+IKKAK  +LLLTAGIGSDHIDL AAAAAGLTVA
Sbjct: 80  SELEKHIEDMHVLITTPFHPAYVTAEKIKKAKTPELLLTAGIGSDHIDLPAAAAAGLTVA 139

Query: 147 EVTGSNVVSVAEDELMRILILVRNFLPGHHQVIHGDWNVAGIAYRAYDLEGKTVGTVGAG 206
            VTGSN VSVAEDELMRILIL+RNFLPG+ QV+ G+WNVAGIA+RAYDLEGKTVGTVGAG
Sbjct: 140 RVTGSNTVSVAEDELMRILILLRNFLPGYQQVVKGEWNVAGIAHRAYDLEGKTVGTVGAG 199

Query: 207 RIGKLLLQRLKPFNCNLLYHDRLKIDPELEKEIGAQFEEDLDAMLPKCDVLVINTPLTDK 266
           R G+LLLQRLKPFNCNLLYHDRL+I+PELEKEIGA+FEEDLDAMLPKCDV+VINTPLT+K
Sbjct: 200 RYGRLLLQRLKPFNCNLLYHDRLQINPELEKEIGAKFEEDLDAMLPKCDVVVINTPLTEK 259

Query: 267 TRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVVDACNSGHIGGYSGDVWNPQPAPKDHP 326
           TRG+FNKE+IAK KKGV+IVNNARGAIMDTQAV DAC+SGHI GY GDVW PQPAPKDHP
Sbjct: 260 TRGMFNKEKIAKMKKGVIIVNNARGAIMDTQAVADACSSGHIAGYGGDVWFPQPAPKDHP 319

Query: 327 WRYMPNQAMTPHISGTTIDGQLRYAAGVKDMLERYFKGEEFPAQNYIVKEGELAPQYR 385
           WRYMPN AMTPHISGTTID QLRYAAGVKDML+RYFKGEEFP +NYIVKEGELA QY+
Sbjct: 320 WRYMPNHAMTPHISGTTIDAQLRYAAGVKDMLDRYFKGEEFPVENYIVKEGELASQYK 377

BLAST of Lcy09g019460 vs. ExPASy TrEMBL
Match: A0A6J1F2G8 (Formate dehydrogenase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111441799 PE=3 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 2.1e-211
Identity = 363/384 (94.53%), Postives = 377/384 (98.18%), Query Frame = 0

Query: 1   MAMKQAAKAAIRALSSSVSSQSSPLVRHLHASAGSKKIVGVFYKANEYAAKNPDFVGCAE 60
           MAMKQAA AAIRA SSSV+SQSSPLVRHLHASAGSKKIVGVFYKANEYAA NP+FVGC E
Sbjct: 1   MAMKQAATAAIRAFSSSVTSQSSPLVRHLHASAGSKKIVGVFYKANEYAAMNPNFVGCVE 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120
           G+LGIREWLESQGHQYIVTDDKEGL+CELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL
Sbjct: 61  GSLGIREWLESQGHQYIVTDDKEGLNCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHIDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIH 180
           QLLLTAGIGSDH+DLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVI 
Sbjct: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIK 180

Query: 181 GDWNVAGIAYRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKEIG 240
           G+WNVAGIA+RAYDLEGKT+GTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEK+IG
Sbjct: 181 GEWNVAGIAHRAYDLEGKTIGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           A+FEED+DAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDT+AVV
Sbjct: 241 AKFEEDMDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTEAVV 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDGQLRYAAGVKDMLER 360
           DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTID QLRYAAGVKDML+R
Sbjct: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLDR 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 385
           YFKG+ FPAQNYIVKEGELAPQYR
Sbjct: 361 YFKGQNFPAQNYIVKEGELAPQYR 384

BLAST of Lcy09g019460 vs. ExPASy TrEMBL
Match: A0A6J1E2C8 (Formate dehydrogenase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111025380 PE=3 SV=1)

HSP 1 Score: 743.8 bits (1919), Expect = 3.6e-211
Identity = 366/384 (95.31%), Postives = 375/384 (97.66%), Query Frame = 0

Query: 1   MAMKQAAKAAIRALSSSVSSQSSPLVRHLHASAGSKKIVGVFYKANEYAAKNPDFVGCAE 60
           MAMKQAA  A+RALSSS +SQSS LVRHLHAS GSKKIVGVFYKANEYAA NP+FVGC E
Sbjct: 1   MAMKQAATTAVRALSSSATSQSSSLVRHLHASPGSKKIVGVFYKANEYAALNPNFVGCVE 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120
           GALGIREWLESQGHQYIVTDDKEGLD ELEKHIPDLHVLI+TPFHPAYVTAERIKKAKNL
Sbjct: 61  GALGIREWLESQGHQYIVTDDKEGLDSELEKHIPDLHVLISTPFHPAYVTAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHIDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIH 180
           QLLLTAGIGSDH+DLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVI+
Sbjct: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIN 180

Query: 181 GDWNVAGIAYRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKEIG 240
           G+WNVAGIA+RAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEK+IG
Sbjct: 181 GEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV
Sbjct: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDGQLRYAAGVKDMLER 360
           DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTID QLRYAAGVKDMLER
Sbjct: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 385
           YFKGEEFPAQNYIVKEGELAPQYR
Sbjct: 361 YFKGEEFPAQNYIVKEGELAPQYR 384

BLAST of Lcy09g019460 vs. ExPASy TrEMBL
Match: A0A6J1J712 (Formate dehydrogenase, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111481834 PE=3 SV=1)

HSP 1 Score: 742.3 bits (1915), Expect = 1.0e-210
Identity = 362/384 (94.27%), Postives = 376/384 (97.92%), Query Frame = 0

Query: 1   MAMKQAAKAAIRALSSSVSSQSSPLVRHLHASAGSKKIVGVFYKANEYAAKNPDFVGCAE 60
           MAMKQAA AAIRA SSSV SQSSPLVRHLHASAGSKKIVGVFYKANEYAA NP+F+GC E
Sbjct: 1   MAMKQAATAAIRAFSSSVPSQSSPLVRHLHASAGSKKIVGVFYKANEYAAMNPNFLGCVE 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120
           G+LGIREWLESQGHQYIVTDDKEGL+CELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL
Sbjct: 61  GSLGIREWLESQGHQYIVTDDKEGLNCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHIDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIH 180
           QLLLTAGIGSDH+DLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVI 
Sbjct: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIK 180

Query: 181 GDWNVAGIAYRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKEIG 240
           G+WNVAGIA+RAYDLEGKT+GTVGAGRIGKLLLQRLKPFNC LLYHDRLKIDPELEK+IG
Sbjct: 181 GEWNVAGIAHRAYDLEGKTIGTVGAGRIGKLLLQRLKPFNCKLLYHDRLKIDPELEKQIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           A+FEED+DAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV
Sbjct: 241 AKFEEDMDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDGQLRYAAGVKDMLER 360
           DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTID QLRYAAGVKDML+R
Sbjct: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLDR 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 385
           YFKG++FPAQNYIVKEGELAPQYR
Sbjct: 361 YFKGQDFPAQNYIVKEGELAPQYR 384

BLAST of Lcy09g019460 vs. ExPASy TrEMBL
Match: A0A6J1F3A7 (Formate dehydrogenase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111441799 PE=3 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 3.9e-210
Identity = 360/384 (93.75%), Postives = 377/384 (98.18%), Query Frame = 0

Query: 1   MAMKQAAKAAIRALSSSVSSQSSPLVRHLHASAGSKKIVGVFYKANEYAAKNPDFVGCAE 60
           MAMKQAA AAIRA SSSV+SQSSPLVRHLHASAGSKKIVGVFYKANEYAA NP+FVGC E
Sbjct: 1   MAMKQAATAAIRAFSSSVASQSSPLVRHLHASAGSKKIVGVFYKANEYAAMNPNFVGCVE 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120
           G+LGIREWLESQGHQYIVTDDKEGL+CELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL
Sbjct: 61  GSLGIREWLESQGHQYIVTDDKEGLNCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHIDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIH 180
           +LLLTAGIGSDH+DLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVI 
Sbjct: 121 ELLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIK 180

Query: 181 GDWNVAGIAYRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKEIG 240
           G+WNVAGIA+RAYDLEGKT+GTVGAGRIGKLLLQRLKPFNCNLLYHDRLKI PELEK+IG
Sbjct: 181 GEWNVAGIAHRAYDLEGKTIGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIGPELEKQIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           A+FEED+DAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDT+AVV
Sbjct: 241 AKFEEDMDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTEAVV 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDGQLRYAAGVKDMLER 360
           DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTID QLRYAAGVKDML+R
Sbjct: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLDR 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 385
           YFKG++FPAQNYIVKEG+LAPQYR
Sbjct: 361 YFKGQDFPAQNYIVKEGQLAPQYR 384

BLAST of Lcy09g019460 vs. ExPASy TrEMBL
Match: A0A6J1F8E9 (Formate dehydrogenase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111441799 PE=3 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 3.9e-210
Identity = 360/384 (93.75%), Postives = 377/384 (98.18%), Query Frame = 0

Query: 1   MAMKQAAKAAIRALSSSVSSQSSPLVRHLHASAGSKKIVGVFYKANEYAAKNPDFVGCAE 60
           MAMKQAA AAIRA SSSV+SQSSPLVRHLHASAGSKKIVGVFYKANEYAA NP+FVGC E
Sbjct: 1   MAMKQAATAAIRAFSSSVTSQSSPLVRHLHASAGSKKIVGVFYKANEYAAMNPNFVGCVE 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120
           G+LGIREWLESQGHQYIVTDDKEGL+CELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL
Sbjct: 61  GSLGIREWLESQGHQYIVTDDKEGLNCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHIDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIH 180
           +LLLTAGIGSDH+DLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVI 
Sbjct: 121 ELLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIK 180

Query: 181 GDWNVAGIAYRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKEIG 240
           G+WNVAGIA+RAYDLEGKT+GTVGAGRIGKLLLQRLKPFNCNLLYHDRLKI PELEK+IG
Sbjct: 181 GEWNVAGIAHRAYDLEGKTIGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIGPELEKQIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           A+FEED+DAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDT+AVV
Sbjct: 241 AKFEEDMDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTEAVV 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDGQLRYAAGVKDMLER 360
           DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTID QLRYAAGVKDML+R
Sbjct: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLDR 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 385
           YFKG++FPAQNYIVKEG+LAPQYR
Sbjct: 361 YFKGQDFPAQNYIVKEGQLAPQYR 384

BLAST of Lcy09g019460 vs. NCBI nr
Match: XP_038904948.1 (formate dehydrogenase, mitochondrial [Benincasa hispida])

HSP 1 Score: 747.7 bits (1929), Expect = 5.1e-212
Identity = 368/384 (95.83%), Postives = 378/384 (98.44%), Query Frame = 0

Query: 1   MAMKQAAKAAIRALSSSVSSQSSPLVRHLHASAGSKKIVGVFYKANEYAAKNPDFVGCAE 60
           MAMKQAA AAIRALSSSV+S SSPL+R+LHASAGSKKIVGVFYKANEYAA NP+FVGC E
Sbjct: 1   MAMKQAATAAIRALSSSVTSHSSPLLRNLHASAGSKKIVGVFYKANEYAALNPNFVGCVE 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120
           GALGIREWLESQGHQYIVTDDKEGLDCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL
Sbjct: 61  GALGIREWLESQGHQYIVTDDKEGLDCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHIDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIH 180
           QLLLTAGIGSDH+DLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVI+
Sbjct: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIN 180

Query: 181 GDWNVAGIAYRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKEIG 240
           G+WNVAGIA+RAYDLEGKTVGTVGAGRIG+LLLQRLKPFNCNLLYHDRLKID ELEK+IG
Sbjct: 181 GEWNVAGIAHRAYDLEGKTVGTVGAGRIGRLLLQRLKPFNCNLLYHDRLKIDSELEKQIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV
Sbjct: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDGQLRYAAGVKDMLER 360
           DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTID QLRYAAGVKDMLER
Sbjct: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 385
           YFKGEEFPAQNYIVKEGELAPQYR
Sbjct: 361 YFKGEEFPAQNYIVKEGELAPQYR 384

BLAST of Lcy09g019460 vs. NCBI nr
Match: XP_022934681.1 (formate dehydrogenase, mitochondrial isoform X3 [Cucurbita moschata])

HSP 1 Score: 744.6 bits (1921), Expect = 4.3e-211
Identity = 363/384 (94.53%), Postives = 377/384 (98.18%), Query Frame = 0

Query: 1   MAMKQAAKAAIRALSSSVSSQSSPLVRHLHASAGSKKIVGVFYKANEYAAKNPDFVGCAE 60
           MAMKQAA AAIRA SSSV+SQSSPLVRHLHASAGSKKIVGVFYKANEYAA NP+FVGC E
Sbjct: 1   MAMKQAATAAIRAFSSSVTSQSSPLVRHLHASAGSKKIVGVFYKANEYAAMNPNFVGCVE 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120
           G+LGIREWLESQGHQYIVTDDKEGL+CELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL
Sbjct: 61  GSLGIREWLESQGHQYIVTDDKEGLNCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHIDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIH 180
           QLLLTAGIGSDH+DLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVI 
Sbjct: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIK 180

Query: 181 GDWNVAGIAYRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKEIG 240
           G+WNVAGIA+RAYDLEGKT+GTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEK+IG
Sbjct: 181 GEWNVAGIAHRAYDLEGKTIGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           A+FEED+DAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDT+AVV
Sbjct: 241 AKFEEDMDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTEAVV 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDGQLRYAAGVKDMLER 360
           DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTID QLRYAAGVKDML+R
Sbjct: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLDR 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 385
           YFKG+ FPAQNYIVKEGELAPQYR
Sbjct: 361 YFKGQNFPAQNYIVKEGELAPQYR 384

BLAST of Lcy09g019460 vs. NCBI nr
Match: KAG6581029.1 (Formate dehydrogenase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 744.2 bits (1920), Expect = 5.6e-211
Identity = 362/384 (94.27%), Postives = 378/384 (98.44%), Query Frame = 0

Query: 1   MAMKQAAKAAIRALSSSVSSQSSPLVRHLHASAGSKKIVGVFYKANEYAAKNPDFVGCAE 60
           MAMKQAA AAIRA SSSV+SQSSPLVRHLHASAGSKKIVGVFYKANEYAA NP+FVGC E
Sbjct: 1   MAMKQAATAAIRAFSSSVASQSSPLVRHLHASAGSKKIVGVFYKANEYAAMNPNFVGCVE 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120
           G+LGIREWLESQGHQYIVTDDKEGL+CELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL
Sbjct: 61  GSLGIREWLESQGHQYIVTDDKEGLNCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHIDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIH 180
           +LLLTAGIGSDH+DLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVI 
Sbjct: 121 ELLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIK 180

Query: 181 GDWNVAGIAYRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKEIG 240
           G+WNVAGIA+RAYDLEGKT+GTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEK+IG
Sbjct: 181 GEWNVAGIAHRAYDLEGKTIGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           A+FEED+DAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDT+AVV
Sbjct: 241 AKFEEDMDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTEAVV 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDGQLRYAAGVKDMLER 360
           DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTID QLRYAAGVKDML+R
Sbjct: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLDR 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 385
           YFKG++FPAQNYIVKEGELAPQYR
Sbjct: 361 YFKGQDFPAQNYIVKEGELAPQYR 384

BLAST of Lcy09g019460 vs. NCBI nr
Match: XP_022158921.1 (formate dehydrogenase, mitochondrial [Momordica charantia])

HSP 1 Score: 743.8 bits (1919), Expect = 7.4e-211
Identity = 366/384 (95.31%), Postives = 375/384 (97.66%), Query Frame = 0

Query: 1   MAMKQAAKAAIRALSSSVSSQSSPLVRHLHASAGSKKIVGVFYKANEYAAKNPDFVGCAE 60
           MAMKQAA  A+RALSSS +SQSS LVRHLHAS GSKKIVGVFYKANEYAA NP+FVGC E
Sbjct: 1   MAMKQAATTAVRALSSSATSQSSSLVRHLHASPGSKKIVGVFYKANEYAALNPNFVGCVE 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120
           GALGIREWLESQGHQYIVTDDKEGLD ELEKHIPDLHVLI+TPFHPAYVTAERIKKAKNL
Sbjct: 61  GALGIREWLESQGHQYIVTDDKEGLDSELEKHIPDLHVLISTPFHPAYVTAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHIDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIH 180
           QLLLTAGIGSDH+DLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVI+
Sbjct: 121 QLLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIN 180

Query: 181 GDWNVAGIAYRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKEIG 240
           G+WNVAGIA+RAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEK+IG
Sbjct: 181 GEWNVAGIAHRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV
Sbjct: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDGQLRYAAGVKDMLER 360
           DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTID QLRYAAGVKDMLER
Sbjct: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLER 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 385
           YFKGEEFPAQNYIVKEGELAPQYR
Sbjct: 361 YFKGEEFPAQNYIVKEGELAPQYR 384

BLAST of Lcy09g019460 vs. NCBI nr
Match: XP_023527059.1 (formate dehydrogenase, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 743.4 bits (1918), Expect = 9.6e-211
Identity = 362/384 (94.27%), Postives = 377/384 (98.18%), Query Frame = 0

Query: 1   MAMKQAAKAAIRALSSSVSSQSSPLVRHLHASAGSKKIVGVFYKANEYAAKNPDFVGCAE 60
           MAMKQAA AAIRA SSSV SQSSPLVRHLHASAGSKKIVGVFYKANEYAA NP+FVGC E
Sbjct: 1   MAMKQAATAAIRAFSSSVPSQSSPLVRHLHASAGSKKIVGVFYKANEYAAMNPNFVGCVE 60

Query: 61  GALGIREWLESQGHQYIVTDDKEGLDCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120
           G+LGIREWLESQGHQYIVTDDKEGL+CELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL
Sbjct: 61  GSLGIREWLESQGHQYIVTDDKEGLNCELEKHIPDLHVLITTPFHPAYVTAERIKKAKNL 120

Query: 121 QLLLTAGIGSDHIDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIH 180
           +LLLTAGIGSDH+DLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVI 
Sbjct: 121 ELLLTAGIGSDHVDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVIK 180

Query: 181 GDWNVAGIAYRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKEIG 240
           G+WNVAGIA+RAYDLEGKT+GTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEK+IG
Sbjct: 181 GEWNVAGIAHRAYDLEGKTIGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKQIG 240

Query: 241 AQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVV 300
           A+FEED+DAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDT+AVV
Sbjct: 241 AKFEEDMDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTEAVV 300

Query: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDGQLRYAAGVKDMLER 360
           DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTID QLRYAAGVKDML+R
Sbjct: 301 DACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDAQLRYAAGVKDMLDR 360

Query: 361 YFKGEEFPAQNYIVKEGELAPQYR 385
           YFKG++FPAQNYIVKEGELAPQYR
Sbjct: 361 YFKGQDFPAQNYIVKEGELAPQYR 384

BLAST of Lcy09g019460 vs. TAIR 10
Match: AT5G14780.1 (formate dehydrogenase )

HSP 1 Score: 653.7 bits (1685), Expect = 9.4e-188
Identity = 324/385 (84.16%), Postives = 351/385 (91.17%), Query Frame = 0

Query: 1   MAMKQAAKAAIRALSSSVSSQSSPLVRHLHASAG-SKKIVGVFYKANEYAAKNPDFVGCA 60
           MAM+QAAKA IRA SSS SS      R  +AS+G SKKIVGVFYKANEYA KNP+F+GC 
Sbjct: 1   MAMRQAAKATIRACSSS-SSSGYFARRQFNASSGDSKKIVGVFYKANEYATKNPNFLGCV 60

Query: 61  EGALGIREWLESQGHQYIVTDDKEGLDCELEKHIPDLHVLITTPFHPAYVTAERIKKAKN 120
           E ALGIR+WLESQGHQYIVTDDKEG DCELEKHIPDLHVLI+TPFHPAYVTAERIKKAKN
Sbjct: 61  ENALGIRDWLESQGHQYIVTDDKEGPDCELEKHIPDLHVLISTPFHPAYVTAERIKKAKN 120

Query: 121 LQLLLTAGIGSDHIDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVI 180
           L+LLLTAGIGSDHIDL AAAAAGLTVAEVTGSNVVSVAEDELMRILIL+RNF+PG++QV+
Sbjct: 121 LKLLLTAGIGSDHIDLQAAAAAGLTVAEVTGSNVVSVAEDELMRILILMRNFVPGYNQVV 180

Query: 181 HGDWNVAGIAYRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKEI 240
            G+WNVAGIAYRAYDLEGKT+GTVGAGRIGKLLLQRLKPF CNLLYHDRL++ PELEKE 
Sbjct: 181 KGEWNVAGIAYRAYDLEGKTIGTVGAGRIGKLLLQRLKPFGCNLLYHDRLQMAPELEKET 240

Query: 241 GAQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAV 300
           GA+F EDL+ MLPKCDV+VIN PLT+KTRG+FNKE I K KKGVLIVNNARGAIM+ QAV
Sbjct: 241 GAKFVEDLNEMLPKCDVIVINMPLTEKTRGMFNKELIGKLKKGVLIVNNARGAIMERQAV 300

Query: 301 VDACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDGQLRYAAGVKDMLE 360
           VDA  SGHIGGYSGDVW+PQPAPKDHPWRYMPNQAMTPH SGTTID QLRYAAG KDMLE
Sbjct: 301 VDAVESGHIGGYSGDVWDPQPAPKDHPWRYMPNQAMTPHTSGTTIDAQLRYAAGTKDMLE 360

Query: 361 RYFKGEEFPAQNYIVKEGELAPQYR 385
           RYFKGE+FP +NYIVK+GELAPQYR
Sbjct: 361 RYFKGEDFPTENYIVKDGELAPQYR 384

BLAST of Lcy09g019460 vs. TAIR 10
Match: AT1G17745.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 117.9 bits (294), Expect = 1.8e-26
Identity = 79/279 (28.32%), Postives = 133/279 (47.67%), Query Frame = 0

Query: 88  ELEKHIPDLHVLITTPFHPAYVTAERIKKAK-NLQLLLTAGIGSDHIDLNAAAAAGLTVA 147
           +L+K + +   LI        VT E  + AK  L+++  AG+G D++DL AA   G  V 
Sbjct: 115 DLKKKVAESDALIVR--SGTKVTREVFEAAKGRLKVVGRAGVGIDNVDLQAATEHGCLVV 174

Query: 148 EVTGSNVVSVAEDELMRILILVRNFLPGHHQVIHGDWNVAGIAYRAYDLEGKTVGTVGAG 207
               +N V+ AE  +  +  + RN       +  G W  +   Y    L GKT+  +G G
Sbjct: 175 NAPTANTVAAAEHGIALLASMARNVAQADASIKAGKWERS--KYVGVSLVGKTLAVMGFG 234

Query: 208 RIGKLLLQRLKPFNCNLLYHDRLKIDPELEKEIGAQFEEDLDAMLPKCDVLVINTPLTDK 267
           ++G  + +R K     ++ HD      +  + +G       D  +   D + ++ PLT  
Sbjct: 235 KVGTEVARRAKGLGMTVISHDPY-APADRARALGVDL-VSFDQAISTADFVSLHMPLTPA 294

Query: 268 TRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVVDACNSGHIGGYSGDVWNPQPAPKDHP 327
           T+ +FN E  +K KKGV ++N ARG ++D  A+V A ++G +   + DV+  +P  KD  
Sbjct: 295 TKKVFNDETFSKMKKGVRLINVARGGVIDEDALVRALDAGIVAQAALDVFCEEPPSKDSR 354

Query: 328 WRYMPNQAMTPHISGTTIDGQLRYAAGVKDMLERYFKGE 366
                N  +TPH+  +T + Q   A  + + +    KGE
Sbjct: 355 LIQHENVTVTPHLGASTKEAQEGVAIEIAEAVAGALKGE 387

BLAST of Lcy09g019460 vs. TAIR 10
Match: AT4G34200.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 112.1 bits (279), Expect = 1.0e-24
Identity = 72/246 (29.27%), Postives = 116/246 (47.15%), Query Frame = 0

Query: 120 LQLLLTAGIGSDHIDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILILVRNFLPGHHQVI 179
           L+++  AG+G D++DL+AA   G  V     +N ++ AE  +  +  + RN       V 
Sbjct: 125 LKVVGRAGVGIDNVDLSAATEFGCLVVNAPTANTIAAAEHGIALMAAMARNVAQADASVK 184

Query: 180 HGDWNVAGIAYRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKEI 239
            G+W      Y    L GKT+  +G G++G  + +R K     ++ HD      +    I
Sbjct: 185 AGEWK--RNKYVGVSLVGKTLAVLGFGKVGTEVARRAKGLGMRVIAHDPY-APADRAHAI 244

Query: 240 GAQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAV 299
           G       D  L   D + ++ PLT  T  + N E  AK KKGV IVN ARG ++D  A+
Sbjct: 245 GVDL-VSFDEALATADFISLHMPLTPTTSKILNDETFAKMKKGVRIVNVARGGVIDEDAL 304

Query: 300 VDACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDGQLRYAAGVKDMLE 359
           V A ++G +   + DV+  +P  KD          +TPH+  +T++ Q   A  + + + 
Sbjct: 305 VRALDAGIVAQAALDVFTKEPPAKDSKLVQHERVTVTPHLGASTMEAQEGVAIEIAEAVV 364

Query: 360 RYFKGE 366
               GE
Sbjct: 365 GALNGE 366

BLAST of Lcy09g019460 vs. TAIR 10
Match: AT2G45630.2 (D-isomer specific 2-hydroxyacid dehydrogenase family protein )

HSP 1 Score: 109.0 bits (271), Expect = 8.6e-24
Identity = 77/260 (29.62%), Postives = 121/260 (46.54%), Query Frame = 0

Query: 107 AYVTAERIKKAKNLQLLLTAGIGSDHIDLNAAAAAGLTVAEVTGSNVVSVAEDELMRILI 166
           A VTA+ I+   NL+L++T   G DH+DL      G++VA    S    VA+  +  ++ 
Sbjct: 78  APVTADLIRILPNLRLVVTTSAGVDHVDLVECRRRGISVANAGSSFSEDVADTAVGLLID 137

Query: 167 LVRNFLPGHHQVIHGDWNVAGIAYRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYH 226
           + R     +  V    W + G       L  K +G VG G IG  +  RL  F C + Y 
Sbjct: 138 VFRRISAANRFVKQRFWPLKGDYPLGSKLGRKRIGIVGLGSIGSKVATRLDAFGCQISYS 197

Query: 227 DRLKIDPELEKEIGAQFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIV 286
            R +       ++   +  D++ M    D L+I   L +KT  L NK+ ++   K  +IV
Sbjct: 198 SRNR----KPYDVPYHYYMDIEEMAANSDALIICCELNEKTLRLINKDVLSALGKRGVIV 257

Query: 287 NNARGAIMDTQAVVDACNSGHIGGYSGDVWNPQP-APKDHPWRYMPNQAMTPHISGTTID 346
           N ARGAI+D + +V     G IGG   DV+  +P  PK+     + N   +PH +  T++
Sbjct: 258 NVARGAIIDEEEMVRCLREGEIGGAGLDVFEDEPNVPKE--LFELDNVVFSPHSAFMTLE 317

Query: 347 GQLRYAAGVKDMLERYFKGE 366
           G       V   +E +F  +
Sbjct: 318 GLEELGKVVVGNIEAFFSNK 331

BLAST of Lcy09g019460 vs. TAIR 10
Match: AT1G17745.2 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 105.9 bits (263), Expect = 7.3e-23
Identity = 79/304 (25.99%), Postives = 133/304 (43.75%), Query Frame = 0

Query: 88  ELEKHIPDLHVLITTPFHPAYVTAERIKKAK-NLQLLLTAGIGSDHIDLNAAAAAGLTVA 147
           +L+K + +   LI        VT E  + AK  L+++  AG+G D++DL AA   G  V 
Sbjct: 115 DLKKKVAESDALIVR--SGTKVTREVFEAAKGRLKVVGRAGVGIDNVDLQAATEHGCLVV 174

Query: 148 EVTGSNVVSVAEDELMRILILVRNFLPGHHQVIHGDWNVAGIA----------------- 207
               +N V+ AE  +  +  + RN       +  G  N   +                  
Sbjct: 175 NAPTANTVAAAEHGIALLASMARNVAQADASIKAGTLNYLFLVLLLRWNCRQSKHQYTIE 234

Query: 208 --------YRAYDLEGKTVGTVGAGRIGKLLLQRLKPFNCNLLYHDRLKIDPELEKEIGA 267
                   Y    L GKT+  +G G++G  + +R K     ++ HD      +  + +G 
Sbjct: 235 TETEKRSKYVGVSLVGKTLAVMGFGKVGTEVARRAKGLGMTVISHDPY-APADRARALGV 294

Query: 268 QFEEDLDAMLPKCDVLVINTPLTDKTRGLFNKERIAKCKKGVLIVNNARGAIMDTQAVVD 327
                 D  +   D + ++ PLT  T+ +FN E  +K KKGV ++N ARG ++D  A+V 
Sbjct: 295 DL-VSFDQAISTADFVSLHMPLTPATKKVFNDETFSKMKKGVRLINVARGGVIDEDALVR 354

Query: 328 ACNSGHIGGYSGDVWNPQPAPKDHPWRYMPNQAMTPHISGTTIDGQLRYAAGVKDMLERY 366
           A ++G +   + DV+  +P  KD       N  +TPH+  +T + Q   A  + + +   
Sbjct: 355 ALDAGIVAQAALDVFCEEPPSKDSRLIQHENVTVTPHLGASTKEAQEGVAIEIAEAVAGA 414

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q075113.7e-18984.38Formate dehydrogenase, mitochondrial OS=Solanum tuberosum OX=4113 GN=FDH1 PE=1 S... [more]
Q9S7E41.3e-18684.16Formate dehydrogenase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=37... [more]
Q9SXP22.5e-18583.16Formate dehydrogenase 1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q67U697.5e-18279.69Formate dehydrogenase 2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 ... [more]
Q9ZRI81.3e-18185.20Formate dehydrogenase, mitochondrial OS=Hordeum vulgare OX=4513 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1F2G82.1e-21194.53Formate dehydrogenase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC1114417... [more]
A0A6J1E2C83.6e-21195.31Formate dehydrogenase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111025... [more]
A0A6J1J7121.0e-21094.27Formate dehydrogenase, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111481834... [more]
A0A6J1F3A73.9e-21093.75Formate dehydrogenase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC1114417... [more]
A0A6J1F8E93.9e-21093.75Formate dehydrogenase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC1114417... [more]
Match NameE-valueIdentityDescription
XP_038904948.15.1e-21295.83formate dehydrogenase, mitochondrial [Benincasa hispida][more]
XP_022934681.14.3e-21194.53formate dehydrogenase, mitochondrial isoform X3 [Cucurbita moschata][more]
KAG6581029.15.6e-21194.27Formate dehydrogenase, mitochondrial, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_022158921.17.4e-21195.31formate dehydrogenase, mitochondrial [Momordica charantia][more]
XP_023527059.19.6e-21194.27formate dehydrogenase, mitochondrial [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G14780.19.4e-18884.16formate dehydrogenase [more]
AT1G17745.11.8e-2628.32D-3-phosphoglycerate dehydrogenase [more]
AT4G34200.11.0e-2429.27D-3-phosphoglycerate dehydrogenase [more]
AT2G45630.28.6e-2429.62D-isomer specific 2-hydroxyacid dehydrogenase family protein [more]
AT1G17745.27.3e-2325.99D-3-phosphoglycerate dehydrogenase [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 37..380
e-value: 1.2E-162
score: 542.8
NoneNo IPR availableGENE3D3.40.50.720coord: 152..342
e-value: 1.2E-162
score: 542.8
NoneNo IPR availablePANTHERPTHR42938:SF26FORMATE DEHYDROGENASE CHLOROPLASTIC/MITOCHONDRIALcoord: 25..383
NoneNo IPR availablePANTHERPTHR42938FORMATE DEHYDROGENASE 1coord: 25..383
NoneNo IPR availableSUPERFAMILY52283Formate/glycerate dehydrogenase catalytic domain-likecoord: 47..173
IPR006140D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domainPFAMPF028262-Hacid_dh_Ccoord: 162..340
e-value: 1.9E-51
score: 173.9
IPR006139D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domainPFAMPF003892-Hacid_dhcoord: 69..366
e-value: 1.5E-14
score: 55.0
IPR029753D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved sitePROSITEPS00671D_2_HYDROXYACID_DH_3coord: 279..295
IPR029753D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved sitePROSITEPS00670D_2_HYDROXYACID_DH_2coord: 250..272
IPR029752D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved site 1PROSITEPS00065D_2_HYDROXYACID_DH_1coord: 200..227
IPR033689NAD-dependent formate dehydrogenaseHAMAPMF_03210Formate_dehydrogenasecoord: 36..384
score: 58.863251
IPR033689NAD-dependent formate dehydrogenaseCDDcd05302FDHcoord: 36..383
e-value: 0.0
score: 648.232
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 155..341

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g019460.1Lcy09g019460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042183 formate catabolic process
cellular_component GO:0009507 chloroplast
cellular_component GO:0009326 formate dehydrogenase complex
cellular_component GO:0005739 mitochondrion
molecular_function GO:0008863 formate dehydrogenase (NAD+) activity
molecular_function GO:0051287 NAD binding
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor