Lcy09g009550 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy09g009550
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Polcalcin Jun o
Location	Chr09: 9737008 .. 9738055 (+)
RNA-Seq Expression	Lcy09g009550
Synteny	Lcy09g009550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCGCAATCCCTTTCTCTCTCTAAATTTGGAAAATCAAATTTCCATGGGATTTCGATTTCAATCTGTTACGAATTCCAATTCCTTCTCCGATTCTGTTTCTTCCAAATCCCGCAATTCTCTTTCCCCCCGCCCTCGCCCCCGCCCCCGCCCCCGCCCATATATAATCTCCGATCCCTCCCCGATTCGCCATCTGTTTCCGCTTCCATCCACTTTTAGTTTTCCCCCTTTTAATTCCGAAACTGAAGACCCAATCTCTCTCACTTCAACTCATGGGATTCAAATCTCTCTTCTCCCGGAAGAAGAAATCCGACACCCCTGGCGCTGGCGATTCACCTCCCGGATCTACCACGGCGGTTCCGATTCTCGGATCTCGAACGCCCTCTCTCAATTCGGGAGCGCAAATCGCGGAACTCGAGCAAGTATTCAAGAAATTCGATGTCAATGGCGACGGCAAGATCTGCTCCTCTGAACTCGGATCCATCATGGGGAGCCTAGGCCAGCCGGCCACCGAGGAGGAGCTTCAGAACATGATCAAGGAGGTCGATGCCGACGGCGACGGCTACATTGACCTCGACGAGTTCATCGAACTCAACACCAAGGGCGTCGATTCCAACGAGGTGCTCGAGAATCTGAAGGACGCATTCTCCGTCTACGACATCGACGGCAACGGATCCATCACGGCCGAGGAATTGCACGAGGTCTTGAAGAGTTTGGGCGACGAGTGCTCCTTGGCGGATTGCCGGAAGATGATCACCGGCGTCGACAGAAACGGCGACGGTATGATTAGTTTCGATGAATTCAAAGTCATGATGATGAGCGGTTCTCGATCGCACGGCTTCAGTGGTTAATTAATTAATTAATACTAATTGATAAAAATTATCAGTTAATTAGCTATTTTGCTAACATTTTCCGTTCTTTCATTATCTAATTAGGAATTATTTGCCTGTAATTTATTTTTAATTCTAATTAAAAAAAGAAATTAGTAGACCTCGCAATTTTCTTTCCCTTTTAATTTTTAATTAATAGAATTAATTGGGGCAGTTAGGAA

mRNA sequence

GCGCAATCCCTTTCTCTCTCTAAATTTGGAAAATCAAATTTCCATGGGATTTCGATTTCAATCTGTTACGAATTCCAATTCCTTCTCCGATTCTGTTTCTTCCAAATCCCGCAATTCTCTTTCCCCCCGCCCTCGCCCCCGCCCCCGCCCCCGCCCATATATAATCTCCGATCCCTCCCCGATTCGCCATCTGTTTCCGCTTCCATCCACTTTTAGTTTTCCCCCTTTTAATTCCGAAACTGAAGACCCAATCTCTCTCACTTCAACTCATGGGATTCAAATCTCTCTTCTCCCGGAAGAAGAAATCCGACACCCCTGGCGCTGGCGATTCACCTCCCGGATCTACCACGGCGGTTCCGATTCTCGGATCTCGAACGCCCTCTCTCAATTCGGGAGCGCAAATCGCGGAACTCGAGCAAGTATTCAAGAAATTCGATGTCAATGGCGACGGCAAGATCTGCTCCTCTGAACTCGGATCCATCATGGGGAGCCTAGGCCAGCCGGCCACCGAGGAGGAGCTTCAGAACATGATCAAGGAGGTCGATGCCGACGGCGACGGCTACATTGACCTCGACGAGTTCATCGAACTCAACACCAAGGGCGTCGATTCCAACGAGGTGCTCGAGAATCTGAAGGACGCATTCTCCGTCTACGACATCGACGGCAACGGATCCATCACGGCCGAGGAATTGCACGAGGTCTTGAAGAGTTTGGGCGACGAGTGCTCCTTGGCGGATTGCCGGAAGATGATCACCGGCGTCGACAGAAACGGCGACGGTATGATTAGTTTCGATGAATTCAAAGTCATGATGATGAGCGGTTCTCGATCGCACGGCTTCAGTGGTTAATTAATTAATTAATACTAATTGATAAAAATTATCAGTTAATTAGCTATTTTGCTAACATTTTCCGTTCTTTCATTATCTAATTAGGAATTATTTGCCTGTAATTTATTTTTAATTCTAATTAAAAAAAGAAATTAGTAGACCTCGCAATTTTCTTTCCCTTTTAATTTTTAATTAATAGAATTAATTGGGGCAGTTAGGAA

Coding sequence (CDS)

ATGGGATTCAAATCTCTCTTCTCCCGGAAGAAGAAATCCGACACCCCTGGCGCTGGCGATTCACCTCCCGGATCTACCACGGCGGTTCCGATTCTCGGATCTCGAACGCCCTCTCTCAATTCGGGAGCGCAAATCGCGGAACTCGAGCAAGTATTCAAGAAATTCGATGTCAATGGCGACGGCAAGATCTGCTCCTCTGAACTCGGATCCATCATGGGGAGCCTAGGCCAGCCGGCCACCGAGGAGGAGCTTCAGAACATGATCAAGGAGGTCGATGCCGACGGCGACGGCTACATTGACCTCGACGAGTTCATCGAACTCAACACCAAGGGCGTCGATTCCAACGAGGTGCTCGAGAATCTGAAGGACGCATTCTCCGTCTACGACATCGACGGCAACGGATCCATCACGGCCGAGGAATTGCACGAGGTCTTGAAGAGTTTGGGCGACGAGTGCTCCTTGGCGGATTGCCGGAAGATGATCACCGGCGTCGACAGAAACGGCGACGGTATGATTAGTTTCGATGAATTCAAAGTCATGATGATGAGCGGTTCTCGATCGCACGGCTTCAGTGGTTAA

Protein sequence

MGFKSLFSRKKKSDTPGAGDSPPGSTTAVPILGSRTPSLNSGAQIAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSRSHGFSG

Homology

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BLAST of Lcy09g009550 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 7.1e-56
Identity = 113/173 (65.32%), Postives = 134/173 (77.46%), Query Frame = 0


Query: 24  GSTTAVPILGSRTPSLNSG----AQIAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPA 83
           GS++ V I G+ +P L        +I ELE VFKKFDVNGDGKI S ELG+IM SLG   
Sbjct: 11  GSSSNVGI-GADSPYLQKARSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEV 70

Query: 84  TEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDAFSVYDIDGNGSITAE 143
            EEEL+  I E+D  GDGYI+ +EF+ELNTKG+D N+VLENLKDAFSVYDIDGNGSI+AE
Sbjct: 71  PEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAE 130

Query: 144 ELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSRSHGFSG 193
           ELHEVL+SLGDECS+A+CRKMI GVD++GDG I F+EFK+MM  GSR     G
Sbjct: 131 ELHEVLRSLGDECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTMGSRRDNVMG 182

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BLAST of Lcy09g009550 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 6.0e-39
Identity = 90/181 (49.72%), Postives = 119/181 (65.75%), Query Frame = 0


Query: 5   SLFSRKKKSDTPGAGDSPPGSTTAVPILGSRTPSLNSGAQIAELEQVFKKFDVNGDGKIC 64
           +LF R+  S +P    + P S    P   + TP         E+E+VF+KFD NGDG+I 
Sbjct: 8   ALFRRRSGSKSPPLPQADPASGGGSP---APTPE-------EEMERVFRKFDANGDGRIS 67

Query: 65  SSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELN-TKGVDSNEVLENLKD 124
            SELG++  SLG  AT++EL  M+ E DADGDG+I LDEF  LN T   D+  V E+L+ 
Sbjct: 68  RSELGALFESLGHAATDDELARMMAEADADGDGFISLDEFAALNATASGDAAAVEEDLRH 127

Query: 125 AFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMS 184
           AF V+D DGNG+I+A EL  VL  LG++ ++  CR+MI GVD+NGDG+ISF+EFKVMM  
Sbjct: 128 AFRVFDADGNGTISAAELARVLHGLGEKATVQQCRRMIEGVDQNGDGLISFEEFKVMMAG 178

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BLAST of Lcy09g009550 vs. ExPASy Swiss-Prot
Match: O64943 (Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2)

HSP 1 Score: 147.1 bits (370), Expect = 2.0e-34
Identity = 72/139 (51.80%), Postives = 100/139 (71.94%), Query Frame = 0


Query: 45  IAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEF 104
           + ELE+VFKKFD NGDGKI  SEL  I+ SLG    E E++ M++E DADGDGY+ L EF
Sbjct: 24  VHELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEVKAMMEEADADGDGYVSLQEF 83

Query: 105 IELNTKGVDSNEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGV 164
           ++LN KG      +++LK+AF V+D D NGSI+A EL   L+S+G+ C++ + + +I  V
Sbjct: 84  VDLNNKGAS----VKDLKNAFKVFDRDCNGSISAAELCHTLESVGEPCTIEESKNIIHNV 143

Query: 165 DRNGDGMISFDEFKVMMMS 184
           D+NGDG+IS +EF+ MM S
Sbjct: 144 DKNGDGLISVEEFQTMMTS 158

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BLAST of Lcy09g009550 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 2.6e-34
Identity = 70/151 (46.36%), Postives = 106/151 (70.20%), Query Frame = 0


Query: 34  SRTPSLNSGAQIAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDA 93
           S+  S N    + ++++VF++FD N DGKI   EL  ++G+L   A++EE + M+KE D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 94  DGDGYIDLDEFIEL--NTKGVDSNEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDE 153
           DG+G+IDLDEF+ L   +    +N  + +LK+AF +YD+D NG I+A ELH V+K+LG++
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 154 CSLADCRKMITGVDRNGDGMISFDEFKVMMM 183
           CS+ DC++MI  VD +GDG + F+EFK MMM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMMM 152

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BLAST of Lcy09g009550 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 146.7 bits (369), Expect = 2.6e-34
Identity = 69/142 (48.59%), Postives = 105/142 (73.94%), Query Frame = 0


Query: 47  ELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIE 106
           ++++VF++FD NGDGKI   EL  ++ +L   A+ EE   M+K+ D DG+G+IDLDEF+ 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 107 LNTKGV----DSNEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMIT 166
           L   G+    ++   + +LK+AF +YD+DGNG I+A+ELH V+K+LG++CS+ DC+KMI+
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 167 GVDRNGDGMISFDEFKVMMMSG 185
            VD +GDG ++FDEFK MM +G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNG 158

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BLAST of Lcy09g009550 vs. ExPASy TrEMBL
Match: A0A6J1CZ20 (probable calcium-binding protein CML25 OS=Momordica charantia OX=3673 GN=LOC111015568 PE=4 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 7.8e-90
Identity = 176/192 (91.67%), Postives = 180/192 (93.75%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTPGAGDSPPGSTTAVPILGSRTPSLNSGAQIAELEQVFKKFDVNGD 60
           MGFKSLFSRKKKSD   + DSPPGS TAVP+LGSRTPS N   QIAELEQVFKKFDVNGD
Sbjct: 1   MGFKSLFSRKKKSD--ASSDSPPGS-TAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGD 60

Query: 61  GKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLEN 120
           GKI SSELGSIMGSLGQPATEEE+QNMIKEVDADGDG+IDLDEFIELNTKGVDS EVLEN
Sbjct: 61  GKISSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLEN 120

Query: 121 LKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVM 180
           LKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVM
Sbjct: 121 LKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVM 180

Query: 181 MMSGSRSHGFSG 193
           MMSGSRSHGF G
Sbjct: 181 MMSGSRSHGFGG 189

[Close]

BLAST of Lcy09g009550 vs. ExPASy TrEMBL
Match: A0A6J1JTS9 (probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC111487441 PE=4 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 9.5e-88
Identity = 173/191 (90.58%), Postives = 180/191 (94.24%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTPGAGDSPPGSTTAVPILGSRTPSLNSGAQIAELEQVFKKFDVNGD 60
           MGFKSLFSRKKKSD+  + DSP GSTT VPILGSRTPS NS AQIAELEQVFKKFDVNGD
Sbjct: 1   MGFKSLFSRKKKSDS--SSDSPLGSTT-VPILGSRTPSFNSRAQIAELEQVFKKFDVNGD 60

Query: 61  GKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLEN 120
           GKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDL EFIELNTKGVDS+EVL+N
Sbjct: 61  GKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKN 120

Query: 121 LKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVM 180
           LKDAFSVYD+DGNGSITAEELHEVLKSLGD+CSLADCR MI+GVDRNGDGMISFDEFK M
Sbjct: 121 LKDAFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAM 180

Query: 181 MMSGSRSHGFS 192
           MMSGSRSHG S
Sbjct: 181 MMSGSRSHGIS 188

[Close]

BLAST of Lcy09g009550 vs. ExPASy TrEMBL
Match: A0A6J1HID9 (probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC111463873 PE=4 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 9.5e-88
Identity = 173/191 (90.58%), Postives = 180/191 (94.24%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTPGAGDSPPGSTTAVPILGSRTPSLNSGAQIAELEQVFKKFDVNGD 60
           MGFKSLFSRKKKSD+  + DSP GSTT VPILGSRTPS NS AQIAELEQVFKKFDVNGD
Sbjct: 1   MGFKSLFSRKKKSDS--SSDSPLGSTT-VPILGSRTPSFNSRAQIAELEQVFKKFDVNGD 60

Query: 61  GKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLEN 120
           GKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDL EFIELNTKGVDS+EVL+N
Sbjct: 61  GKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKN 120

Query: 121 LKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVM 180
           LKDAFSVYD+DGNGSITAEELHEVLKSLGD+CSLADCR MI+GVDRNGDGMISFDEFK M
Sbjct: 121 LKDAFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAM 180

Query: 181 MMSGSRSHGFS 192
           MMSGSRSHG S
Sbjct: 181 MMSGSRSHGLS 188

[Close]

BLAST of Lcy09g009550 vs. ExPASy TrEMBL
Match: A0A5A7TS48 (Putative calcium-binding protein CML25 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3042G00010 PE=4 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 3.7e-84
Identity = 170/192 (88.54%), Postives = 175/192 (91.15%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTPGAGDSPPGSTTAVPILGSRTPSLNSGAQIAELEQVFKKFDVNGD 60
           MGFKSLFSRKKKS    + DSP GSTT +PI GSRT       QIAELEQVF KFDVNGD
Sbjct: 1   MGFKSLFSRKKKSHP--STDSPLGSTT-LPIFGSRT-------QIAELEQVFNKFDVNGD 60

Query: 61  GKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLEN 120
           GKICSSELGSIMGSLGQPATEEEL NMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLEN
Sbjct: 61  GKICSSELGSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLEN 120

Query: 121 LKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVM 180
           LKDAFSVYDIDGNGSITAEELHEVLKSLGD+CSLADCRKMITGVD+NGDGMISFDEFKVM
Sbjct: 121 LKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVM 180

Query: 181 MMSGSRSHGFSG 193
           MMSGSRSHGF+G
Sbjct: 181 MMSGSRSHGFNG 182

[Close]

BLAST of Lcy09g009550 vs. ExPASy TrEMBL
Match: A0A1S3CSR2 (probable calcium-binding protein CML25 OS=Cucumis melo OX=3656 GN=LOC103504532 PE=4 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 3.7e-84
Identity = 170/192 (88.54%), Postives = 175/192 (91.15%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTPGAGDSPPGSTTAVPILGSRTPSLNSGAQIAELEQVFKKFDVNGD 60
           MGFKSLFSRKKKS    + DSP GSTT +PI GSRT       QIAELEQVF KFDVNGD
Sbjct: 1   MGFKSLFSRKKKSHP--STDSPLGSTT-LPIFGSRT-------QIAELEQVFNKFDVNGD 60

Query: 61  GKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLEN 120
           GKICSSELGSIMGSLGQPATEEEL NMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLEN
Sbjct: 61  GKICSSELGSIMGSLGQPATEEELHNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLEN 120

Query: 121 LKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVM 180
           LKDAFSVYDIDGNGSITAEELHEVLKSLGD+CSLADCRKMITGVD+NGDGMISFDEFKVM
Sbjct: 121 LKDAFSVYDIDGNGSITAEELHEVLKSLGDDCSLADCRKMITGVDKNGDGMISFDEFKVM 180

Query: 181 MMSGSRSHGFSG 193
           MMSGSRSHGF+G
Sbjct: 181 MMSGSRSHGFNG 182

[Close]

BLAST of Lcy09g009550 vs. NCBI nr
Match: XP_022146331.1 (probable calcium-binding protein CML25 [Momordica charantia])

HSP 1 Score: 339.7 bits (870), Expect = 1.6e-89
Identity = 176/192 (91.67%), Postives = 180/192 (93.75%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTPGAGDSPPGSTTAVPILGSRTPSLNSGAQIAELEQVFKKFDVNGD 60
           MGFKSLFSRKKKSD   + DSPPGS TAVP+LGSRTPS N   QIAELEQVFKKFDVNGD
Sbjct: 1   MGFKSLFSRKKKSD--ASSDSPPGS-TAVPVLGSRTPSFNCRTQIAELEQVFKKFDVNGD 60

Query: 61  GKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLEN 120
           GKI SSELGSIMGSLGQPATEEE+QNMIKEVDADGDG+IDLDEFIELNTKGVDS EVLEN
Sbjct: 61  GKISSSELGSIMGSLGQPATEEEIQNMIKEVDADGDGFIDLDEFIELNTKGVDSEEVLEN 120

Query: 121 LKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVM 180
           LKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVM
Sbjct: 121 LKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVM 180

Query: 181 MMSGSRSHGFSG 193
           MMSGSRSHGF G
Sbjct: 181 MMSGSRSHGFGG 189

[Close]

BLAST of Lcy09g009550 vs. NCBI nr
Match: XP_022963580.1 (probable calcium-binding protein CML25 [Cucurbita moschata])

HSP 1 Score: 332.8 bits (852), Expect = 2.0e-87
Identity = 173/191 (90.58%), Postives = 180/191 (94.24%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTPGAGDSPPGSTTAVPILGSRTPSLNSGAQIAELEQVFKKFDVNGD 60
           MGFKSLFSRKKKSD+  + DSP GSTT VPILGSRTPS NS AQIAELEQVFKKFDVNGD
Sbjct: 1   MGFKSLFSRKKKSDS--SSDSPLGSTT-VPILGSRTPSFNSRAQIAELEQVFKKFDVNGD 60

Query: 61  GKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLEN 120
           GKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDL EFIELNTKGVDS+EVL+N
Sbjct: 61  GKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKN 120

Query: 121 LKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVM 180
           LKDAFSVYD+DGNGSITAEELHEVLKSLGD+CSLADCR MI+GVDRNGDGMISFDEFK M
Sbjct: 121 LKDAFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAM 180

Query: 181 MMSGSRSHGFS 192
           MMSGSRSHG S
Sbjct: 181 MMSGSRSHGLS 188

[Close]

BLAST of Lcy09g009550 vs. NCBI nr
Match: XP_022990613.1 (probable calcium-binding protein CML25 [Cucurbita maxima])

HSP 1 Score: 332.8 bits (852), Expect = 2.0e-87
Identity = 173/191 (90.58%), Postives = 180/191 (94.24%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTPGAGDSPPGSTTAVPILGSRTPSLNSGAQIAELEQVFKKFDVNGD 60
           MGFKSLFSRKKKSD+  + DSP GSTT VPILGSRTPS NS AQIAELEQVFKKFDVNGD
Sbjct: 1   MGFKSLFSRKKKSDS--SSDSPLGSTT-VPILGSRTPSFNSRAQIAELEQVFKKFDVNGD 60

Query: 61  GKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLEN 120
           GKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDL EFIELNTKGVDS+EVL+N
Sbjct: 61  GKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKN 120

Query: 121 LKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVM 180
           LKDAFSVYD+DGNGSITAEELHEVLKSLGD+CSLADCR MI+GVDRNGDGMISFDEFK M
Sbjct: 121 LKDAFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAM 180

Query: 181 MMSGSRSHGFS 192
           MMSGSRSHG S
Sbjct: 181 MMSGSRSHGIS 188

[Close]

BLAST of Lcy09g009550 vs. NCBI nr
Match: XP_023513887.1 (probable calcium-binding protein CML25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 331.6 bits (849), Expect = 4.4e-87
Identity = 172/189 (91.01%), Postives = 179/189 (94.71%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTPGAGDSPPGSTTAVPILGSRTPSLNSGAQIAELEQVFKKFDVNGD 60
           MGFKSLFSRKKKSD+  + DSP GSTT VPILGSRTPS NS AQIAELEQVFKKFDVNGD
Sbjct: 1   MGFKSLFSRKKKSDS--SSDSPLGSTT-VPILGSRTPSFNSRAQIAELEQVFKKFDVNGD 60

Query: 61  GKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLEN 120
           GKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDL EFIELNTKGVDS+EVL+N
Sbjct: 61  GKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKN 120

Query: 121 LKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVM 180
           LKDAFSVYD+DGNGSITAEELHEVLKSLGD+CSLADCR MI+GVDRNGDGMISFDEFK M
Sbjct: 121 LKDAFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAM 180

Query: 181 MMSGSRSHG 190
           MMSGSRSHG
Sbjct: 181 MMSGSRSHG 186

[Close]

BLAST of Lcy09g009550 vs. NCBI nr
Match: KAG6601861.1 (putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. sororia] >KAG7032560.1 putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 330.9 bits (847), Expect = 7.5e-87
Identity = 172/191 (90.05%), Postives = 179/191 (93.72%), Query Frame = 0


Query: 1   MGFKSLFSRKKKSDTPGAGDSPPGSTTAVPILGSRTPSLNSGAQIAELEQVFKKFDVNGD 60
           MGFKSLFSRKKKSD+  + DSP GSTT VPILGSRTPS NS AQIAELEQVFKKFDVNGD
Sbjct: 1   MGFKSLFSRKKKSDS--SSDSPLGSTT-VPILGSRTPSFNSRAQIAELEQVFKKFDVNGD 60

Query: 61  GKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLEN 120
           GKICSSELGSIMG LGQPATEEELQNMIKEVDADGDGYIDL EFIELNTKGVDS+EVL+N
Sbjct: 61  GKICSSELGSIMGCLGQPATEEELQNMIKEVDADGDGYIDLHEFIELNTKGVDSDEVLKN 120

Query: 121 LKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVM 180
           LKDAFSVYD+DGNGSITAEELHEVLKSLGD+CSLADCR MI+GVDRNGDGMISFDEFK M
Sbjct: 121 LKDAFSVYDLDGNGSITAEELHEVLKSLGDDCSLADCRNMISGVDRNGDGMISFDEFKAM 180

Query: 181 MMSGSRSHGFS 192
           MMSGSRSHG S
Sbjct: 181 MMSGSRSHGLS 188

[Close]

BLAST of Lcy09g009550 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 218.4 bits (555), Expect = 5.0e-57
Identity = 113/173 (65.32%), Postives = 134/173 (77.46%), Query Frame = 0


Query: 24  GSTTAVPILGSRTPSLNSG----AQIAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPA 83
           GS++ V I G+ +P L        +I ELE VFKKFDVNGDGKI S ELG+IM SLG   
Sbjct: 11  GSSSNVGI-GADSPYLQKARSGKTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEV 70

Query: 84  TEEELQNMIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDAFSVYDIDGNGSITAE 143
            EEEL+  I E+D  GDGYI+ +EF+ELNTKG+D N+VLENLKDAFSVYDIDGNGSI+AE
Sbjct: 71  PEEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAE 130

Query: 144 ELHEVLKSLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSGSRSHGFSG 193
           ELHEVL+SLGDECS+A+CRKMI GVD++GDG I F+EFK+MM  GSR     G
Sbjct: 131 ELHEVLRSLGDECSIAECRKMIGGVDKDGDGTIDFEEFKIMMTMGSRRDNVMG 182

[Close]

BLAST of Lcy09g009550 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 146.7 bits (369), Expect = 1.9e-35
Identity = 70/151 (46.36%), Postives = 106/151 (70.20%), Query Frame = 0


Query: 34  SRTPSLNSGAQIAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDA 93
           S+  S N    + ++++VF++FD N DGKI   EL  ++G+L   A++EE + M+KE D 
Sbjct: 2   SKNVSRNCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 61

Query: 94  DGDGYIDLDEFIEL--NTKGVDSNEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDE 153
           DG+G+IDLDEF+ L   +    +N  + +LK+AF +YD+D NG I+A ELH V+K+LG++
Sbjct: 62  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 121

Query: 154 CSLADCRKMITGVDRNGDGMISFDEFKVMMM 183
           CS+ DC++MI  VD +GDG + F+EFK MMM
Sbjct: 122 CSIQDCQRMINKVDSDGDGCVDFEEFKKMMM 152

[Close]

BLAST of Lcy09g009550 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 146.7 bits (369), Expect = 1.9e-35
Identity = 69/142 (48.59%), Postives = 105/142 (73.94%), Query Frame = 0


Query: 47  ELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQNMIKEVDADGDGYIDLDEFIE 106
           ++++VF++FD NGDGKI   EL  ++ +L   A+ EE   M+K+ D DG+G+IDLDEF+ 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 107 LNTKGV----DSNEVLENLKDAFSVYDIDGNGSITAEELHEVLKSLGDECSLADCRKMIT 166
           L   G+    ++   + +LK+AF +YD+DGNG I+A+ELH V+K+LG++CS+ DC+KMI+
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 167 GVDRNGDGMISFDEFKVMMMSG 185
            VD +GDG ++FDEFK MM +G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNG 158

[Close]

BLAST of Lcy09g009550 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 136.3 bits (342), Expect = 2.5e-32
Identity = 74/159 (46.54%), Postives = 99/159 (62.26%), Query Frame = 0


Query: 27  TAVPILGSRTPSLNSGAQIAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQN 86
           +A P     TP+    A   EL++VF +FD NGDGKI   ELG +  ++G   TE EL  
Sbjct: 3   SANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNR 62

Query: 87  MIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDAFSVYDIDGNGSITAEELHEVLK 146
           +++EVD D DGYI+LDEF  L      + E+    +DAF +YD D NG I+A ELH+VL 
Sbjct: 63  VLEEVDTDRDGYINLDEFSTLCRSSSSAAEI----RDAFDLYDQDKNGLISASELHQVLN 122

Query: 147 SLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSGS 186
            LG  CS+ DC +MI  VD +GDG ++F+EF+ MM S S
Sbjct: 123 RLGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS 157

[Close]

BLAST of Lcy09g009550 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 136.3 bits (342), Expect = 2.5e-32
Identity = 74/159 (46.54%), Postives = 99/159 (62.26%), Query Frame = 0


Query: 27  TAVPILGSRTPSLNSGAQIAELEQVFKKFDVNGDGKICSSELGSIMGSLGQPATEEELQN 86
           +A P     TP+    A   EL++VF +FD NGDGKI   ELG +  ++G   TE EL  
Sbjct: 3   SANPETAKPTPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNR 62

Query: 87  MIKEVDADGDGYIDLDEFIELNTKGVDSNEVLENLKDAFSVYDIDGNGSITAEELHEVLK 146
           +++EVD D DGYI+LDEF  L      + E+    +DAF +YD D NG I+A ELH+VL 
Sbjct: 63  VLEEVDTDRDGYINLDEFSTLCRSSSSAAEI----RDAFDLYDQDKNGLISASELHQVLN 122

Query: 147 SLGDECSLADCRKMITGVDRNGDGMISFDEFKVMMMSGS 186
            LG  CS+ DC +MI  VD +GDG ++F+EF+ MM S S
Sbjct: 123 RLGMSCSVEDCTRMIGPVDADGDGNVNFEEFQKMMTSSS 157

The following BLAST results are available for this feature:

BLAST of Lcy09g009550 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FYK2	7.1e-56	65.32	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]
Q8RZB5	6.0e-39	49.72	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
O64943	2.0e-34	51.80	Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2	[more]
Q9C8Y1	2.6e-34	46.36	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]
P25070	2.6e-34	48.59	Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2	[more]

BLAST of Lcy09g009550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CZ20	7.8e-90	91.67	probable calcium-binding protein CML25 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1JTS9	9.5e-88	90.58	probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC1114874...	[more]
A0A6J1HID9	9.5e-88	90.58	probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A5A7TS48	3.7e-84	88.54	Putative calcium-binding protein CML25 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CSR2	3.7e-84	88.54	probable calcium-binding protein CML25 OS=Cucumis melo OX=3656 GN=LOC103504532 P...	[more]

BLAST of Lcy09g009550 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146331.1	1.6e-89	91.67	probable calcium-binding protein CML25 [Momordica charantia]	[more]
XP_022963580.1	2.0e-87	90.58	probable calcium-binding protein CML25 [Cucurbita moschata]	[more]
XP_022990613.1	2.0e-87	90.58	probable calcium-binding protein CML25 [Cucurbita maxima]	[more]
XP_023513887.1	4.4e-87	91.01	probable calcium-binding protein CML25 [Cucurbita pepo subsp. pepo]	[more]
KAG6601861.1	7.5e-87	90.05	putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. s...	[more]

BLAST of Lcy09g009550 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24620.1	5.0e-57	65.32	EF hand calcium-binding protein family	[more]
AT1G66400.1	1.9e-35	46.36	calmodulin like 23	[more]
AT5G37770.1	1.9e-35	48.59	EF hand calcium-binding protein family	[more]
AT1G18210.1	2.5e-32	46.54	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	2.5e-32	46.54	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 47..75 e-value: 4.4E-6 score: 36.2 coord: 156..184 e-value: 2.9E-5 score: 33.5 coord: 83..111 e-value: 4.2E-5 score: 32.9 coord: 120..148 e-value: 1.3E-5 score: 34.7
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 47..106 e-value: 3.4E-14 score: 53.0 coord: 119..181 e-value: 3.9E-12 score: 46.4
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 152..187 score: 12.281773
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 43..78 score: 15.071314
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 79..114 score: 14.094975
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 116..151 score: 15.461849
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 121..182 e-value: 5.49618E-20 score: 77.5881
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 47..107 e-value: 5.5197E-21 score: 79.8993
None	No IPR available	GENE3D	1.10.238.10		coord: 112..187 e-value: 8.6E-25 score: 88.9 coord: 35..111 e-value: 6.4E-25 score: 89.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..34
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 19..34
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 33..187
None	No IPR available	PANTHER	PTHR23050:SF382	CALCIUM-BINDING PROTEIN CML25-RELATED	coord: 33..187
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 165..177
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 92..104
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 129..141
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 56..68
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 39..183

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy09g009550.1	Lcy09g009550.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding