Lcy09g008280 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g008280
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionChitinase family protein
LocationChr09: 7627038 .. 7628729 (+)
RNA-Seq ExpressionLcy09g008280
SyntenyLcy09g008280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATCCAACTTCTTATTCCTCTCCTTCATCTTACTTCTCGGGATCGGCCTCCCGTCGACAGAGGGTCACCGACGTCGGAGGGAAATATCCCCTGTAGCTTCTATTGTTAGCGAAGAACTTTTTAAGAACTTGTTTTTGCATAAGGATGATAGTGTATGTCCAGCGAATGGGTTCTACACTTACCAAGCTTTCATTGAAGCAACGAAGAGATTTTCCAAATTTGGCACGACAGGGTGTTTGGCAACACGAAAGAGGGAGATTGCAGCGTTTTTGGCTCAGATCTCGCACGAGACAACGGGCGGGTGGGCGACTGCCCCGGATGGACCTTTTGCTTGGGGGTTGTGCTTTAAAGAGGAAGTGAATCCACAAAGCAACTATTGTGATGCTAGTGTTGTGCAGTGGCCTTGTTCTCCGGGCAAGTCCTACAAAGGAAGAGGGCCTATTCAACTATCATGGTAAGAACAAACACAAAGTACCATGCGATTCAAAATGTAGTTATTTGTTTTTAAGTTAAAAGAGACCGAAATTAATATTTTGAAAGTTGACCAAAAAAAAAAAAACTTTTACATTTTTTTTTTTAAATTTGGAACCCAATATATTATTCAAATCTCATCTTAATTTCTAAATTTTCAAATTTGTTCAATTTTGGTTTCTAAAATATCATTATATATATTTTTTTAATTTAAGGACAAAAATAAACGTTTTAAAAGTTTTTAATCAAAATATACATTTTTTAACAAATCAAATACTAAATAGACATTTTGAACGTTTTAGAACTAAAATAAAATAATTTTCAGAATTCGAGAATTAATAAAAGATTTTTATTTTTTGAATTGACCAAAATAAAATTTAAATTTGTAACAATTTAGTTTTTTTTTTTTTGGGAGAAGTGTAACAATTTAGTTTGTGTAATTTAAAACTTACAAATTGTCAAAAAAAAAAAATTTAAAACTTACAACAATTTAAACTTTATTGTAAAAAGAATTCAACCAATAGTTAGAAACAATTATCCCTTTTTTTTTTTTTTTTTTTTTGAGATAAATTATCCCTCTAGTTTAAAAACACATTTAATCCACAACAATTTATCAAAATATATTGAACAATCTGAACAAGAAACCTTCAATACACCCTAATAGAATTTTAAATTGAAGGACTAAAAATCGTAGTAAAGTTCAAAAACTAATTATTACATATTATTACAAGGATGAAATATGTGTTTTTGTTAACTTGAATAGTTGATTAATTTCACTTTTCCAAAAAGAAAAAAAAAATAGTTGATTAATTTCATAGAGCGAGTAACTAAAAAAAGGAAGAACGAGCAGGAATTTCAACTACGGGCCAGCAGGGAAGGCACTGGGATTCGACGGGCTCAAAAACCCAGAGATAGTTGCAGAGGACTCGGCAATCGCCTTCAAGACGGCGCTGTGGTTCTGGATGACGGAGCAGAAGCCGAAGCCGTCGTGTCACGACGTCATGGTCGGCCGATACGTTCCCACAGAGGCCGACAGAGAGGCTAATCGAACTGCGGGATATGGGCTCGTCACCAACATCATCAATGGCGGACTCGAGTGTGGGATTGCCAACGATGCCAGAGTCAACGACCGAATCGGCTTCTTCAACAGATATGCTCAGCTCTTCAACGTCGATACTGGGCCCAACTTGGATTGCCAGAATCAGAAACCCTTCTAG

mRNA sequence

ATGGCATCCAACTTCTTATTCCTCTCCTTCATCTTACTTCTCGGGATCGGCCTCCCGTCGACAGAGGGTCACCGACGTCGGAGGGAAATATCCCCTGTAGCTTCTATTGTTAGCGAAGAACTTTTTAAGAACTTGTTTTTGCATAAGGATGATAGTGTATGTCCAGCGAATGGGTTCTACACTTACCAAGCTTTCATTGAAGCAACGAAGAGATTTTCCAAATTTGGCACGACAGGGTGTTTGGCAACACGAAAGAGGGAGATTGCAGCGTTTTTGGCTCAGATCTCGCACGAGACAACGGGCGGGTGGGCGACTGCCCCGGATGGACCTTTTGCTTGGGGGTTGTGCTTTAAAGAGGAAGTGAATCCACAAAGCAACTATTGTGATGCTAGTGTTGTGCAGTGGCCTTGTTCTCCGGGCAAGTCCTACAAAGGAAGAGGGCCTATTCAACTATCATGGAATTTCAACTACGGGCCAGCAGGGAAGGCACTGGGATTCGACGGGCTCAAAAACCCAGAGATAGTTGCAGAGGACTCGGCAATCGCCTTCAAGACGGCGCTGTGGTTCTGGATGACGGAGCAGAAGCCGAAGCCGTCGTGTCACGACGTCATGGTCGGCCGATACGTTCCCACAGAGGCCGACAGAGAGGCTAATCGAACTGCGGGATATGGGCTCGTCACCAACATCATCAATGGCGGACTCGAGTGTGGGATTGCCAACGATGCCAGAGTCAACGACCGAATCGGCTTCTTCAACAGATATGCTCAGCTCTTCAACGTCGATACTGGGCCCAACTTGGATTGCCAGAATCAGAAACCCTTCTAG

Coding sequence (CDS)

ATGGCATCCAACTTCTTATTCCTCTCCTTCATCTTACTTCTCGGGATCGGCCTCCCGTCGACAGAGGGTCACCGACGTCGGAGGGAAATATCCCCTGTAGCTTCTATTGTTAGCGAAGAACTTTTTAAGAACTTGTTTTTGCATAAGGATGATAGTGTATGTCCAGCGAATGGGTTCTACACTTACCAAGCTTTCATTGAAGCAACGAAGAGATTTTCCAAATTTGGCACGACAGGGTGTTTGGCAACACGAAAGAGGGAGATTGCAGCGTTTTTGGCTCAGATCTCGCACGAGACAACGGGCGGGTGGGCGACTGCCCCGGATGGACCTTTTGCTTGGGGGTTGTGCTTTAAAGAGGAAGTGAATCCACAAAGCAACTATTGTGATGCTAGTGTTGTGCAGTGGCCTTGTTCTCCGGGCAAGTCCTACAAAGGAAGAGGGCCTATTCAACTATCATGGAATTTCAACTACGGGCCAGCAGGGAAGGCACTGGGATTCGACGGGCTCAAAAACCCAGAGATAGTTGCAGAGGACTCGGCAATCGCCTTCAAGACGGCGCTGTGGTTCTGGATGACGGAGCAGAAGCCGAAGCCGTCGTGTCACGACGTCATGGTCGGCCGATACGTTCCCACAGAGGCCGACAGAGAGGCTAATCGAACTGCGGGATATGGGCTCGTCACCAACATCATCAATGGCGGACTCGAGTGTGGGATTGCCAACGATGCCAGAGTCAACGACCGAATCGGCTTCTTCAACAGATATGCTCAGCTCTTCAACGTCGATACTGGGCCCAACTTGGATTGCCAGAATCAGAAACCCTTCTAG

Protein sequence

MASNFLFLSFILLLGIGLPSTEGHRRRREISPVASIVSEELFKNLFLHKDDSVCPANGFYTYQAFIEATKRFSKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEVNPQSNYCDASVVQWPCSPGKSYKGRGPIQLSWNFNYGPAGKALGFDGLKNPEIVAEDSAIAFKTALWFWMTEQKPKPSCHDVMVGRYVPTEADREANRTAGYGLVTNIINGGLECGIANDARVNDRIGFFNRYAQLFNVDTGPNLDCQNQKPF
Homology
BLAST of Lcy09g008280 vs. ExPASy Swiss-Prot
Match: Q5NB11 (Chitinase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht10 PE=2 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 9.0e-113
Identity = 179/244 (73.36%), Postives = 206/244 (84.43%), Query Frame = 0

Query: 31  SPVASIVSEELFKNLFLHKDDSVCPANGFYTYQAFIEATKRFSKFGTTGCLATRKREIAA 90
           SPV+SIVSE+L+ +LFLHKDD+ CPA GFYTY +F+ A  RF +F  TGC   RKRE+AA
Sbjct: 47  SPVSSIVSEQLYASLFLHKDDAACPARGFYTYASFVRAATRFPRFAATGCADARKREVAA 106

Query: 91  FLAQISHETTGGWATAPDGPFAWGLCFKEEVNPQSNYCDASVVQWPCSPGKSYKGRGPIQ 150
           FLAQISHETTGGWATAPDGP+AWGLC+KEE+NPQS+YCDA+  QWPC PGKSY GRGPIQ
Sbjct: 107 FLAQISHETTGGWATAPDGPYAWGLCYKEEINPQSSYCDATDKQWPCYPGKSYHGRGPIQ 166

Query: 151 LSWNFNYGPAGKALGFDGLKNPEIVAEDSAIAFKTALWFWMTEQKPKPSCHDVMVGRYVP 210
           +SWNFNYGPAG+ALGFDGL+NPEIVA  S IAF+TALWFWMT +  KPSCH VMVG Y P
Sbjct: 167 ISWNFNYGPAGQALGFDGLRNPEIVANCSDIAFQTALWFWMTPRDTKPSCHQVMVGEYRP 226

Query: 211 TEADREANRTAGYGLVTNIINGGLECGIANDARVNDRIGFFNRYAQLFNVDTGPNLDCQN 270
             AD  ANRTAG+GLVTNI+NGGLEC  A DARVN+RIGF+ RY Q+  VD GPNLDC++
Sbjct: 227 GPADVAANRTAGFGLVTNIVNGGLECNRAGDARVNNRIGFYRRYCQVLGVDVGPNLDCEH 286

Query: 271 QKPF 275
           Q+PF
Sbjct: 287 QQPF 290

BLAST of Lcy09g008280 vs. ExPASy Swiss-Prot
Match: Q09023 (Endochitinase CH25 OS=Brassica napus OX=3708 PE=2 SV=1)

HSP 1 Score: 347.4 bits (890), Expect = 1.4e-94
Identity = 150/242 (61.98%), Postives = 183/242 (75.62%), Query Frame = 0

Query: 33  VASIVSEELFKNLFLHKDDSVCPANGFYTYQAFIEATKRFSKFGTTGCLATRKREIAAFL 92
           ++ I+S   F ++  H++D+ CPA GFYTY AFI A K F  FGTTG  ATRK+EIAAF 
Sbjct: 71  LSGIISRSQFDDMLKHRNDNACPARGFYTYDAFINAAKSFPGFGTTGDTATRKKEIAAFF 130

Query: 93  AQISHETTGGWATAPDGPFAWGLCFKEEVNPQSNYCDASVVQWPCSPGKSYKGRGPIQLS 152
            Q SHETTGGWATAPDGP++WG CFK+E NP SNYC  S  +WPC+ GKSY GRGP+QLS
Sbjct: 131 GQTSHETTGGWATAPDGPYSWGYCFKQEQNPSSNYCSPS-AEWPCASGKSYYGRGPMQLS 190

Query: 153 WNFNYGPAGKALGFDGLKNPEIVAEDSAIAFKTALWFWMTEQKPKPSCHDVMVGRYVPTE 212
           WN+NYG  G+A+G D L NP++V+ D  IAFK A+WFWMT Q PKPSCH V+VG++ P++
Sbjct: 191 WNYNYGQCGRAIGSDLLNNPDLVSNDPVIAFKAAIWFWMTPQSPKPSCHAVIVGQWQPSD 250

Query: 213 ADREANRTAGYGLVTNIINGGLECGIANDARVNDRIGFFNRYAQLFNVDTGPNLDCQNQK 272
           ADR A R  GYG++TNIINGGLECG   DARV DRIGF+ RY  +  V+ G NLDC NQ+
Sbjct: 251 ADRAAGRVPGYGVITNIINGGLECGRGQDARVADRIGFYQRYCNILGVNPGGNLDCYNQR 310

Query: 273 PF 275
            F
Sbjct: 311 SF 311

BLAST of Lcy09g008280 vs. ExPASy Swiss-Prot
Match: Q7DNA1 (Chitinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht2 PE=1 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 1.6e-93
Identity = 151/242 (62.40%), Postives = 182/242 (75.21%), Query Frame = 0

Query: 33  VASIVSEELFKNLFLHKDDSVCPANGFYTYQAFIEATKRFSKFGTTGCLATRKREIAAFL 92
           V SIV  +LF+ L LH++D  CPA GFYTY+AF+ A   F  FG TG   TRKRE+AAFL
Sbjct: 89  VGSIVPRDLFERLLLHRNDGACPARGFYTYEAFLAAAAAFPAFGGTGNTETRKREVAAFL 148

Query: 93  AQISHETTGGWATAPDGPFAWGLCFKEEVNPQSNYCDASVVQWPCSPGKSYKGRGPIQLS 152
            Q SHETTGGW TAPDGPF+WG CFK+E NP S+YC  S  +WPC+PG+ Y GRGPIQLS
Sbjct: 149 GQTSHETTGGWPTAPDGPFSWGYCFKQEQNPPSDYCQPS-PEWPCAPGRKYYGRGPIQLS 208

Query: 153 WNFNYGPAGKALGFDGLKNPEIVAEDSAIAFKTALWFWMTEQKPKPSCHDVMVGRYVPTE 212
           +NFNYGPAG+A+G D L NP++VA D+ ++FKTALWFWMT Q  KPS HDV+ GR+ P+ 
Sbjct: 209 FNFNYGPAGRAIGVDLLSNPDLVATDATVSFKTALWFWMTPQGNKPSSHDVITGRWAPSP 268

Query: 213 ADREANRTAGYGLVTNIINGGLECGIANDARVNDRIGFFNRYAQLFNVDTGPNLDCQNQK 272
           AD  A R  GYG++TNI+NGGLECG   D RV +RIGF+ RY   F + TG NLDC NQ+
Sbjct: 269 ADAAAGRAPGYGVITNIVNGGLECGHGPDDRVANRIGFYQRYCGAFGIGTGGNLDCYNQR 328

Query: 273 PF 275
           PF
Sbjct: 329 PF 329

BLAST of Lcy09g008280 vs. ExPASy Swiss-Prot
Match: P19171 (Basic endochitinase B OS=Arabidopsis thaliana OX=3702 GN=CHI-B PE=1 SV=3)

HSP 1 Score: 342.4 bits (877), Expect = 4.6e-93
Identity = 147/242 (60.74%), Postives = 182/242 (75.21%), Query Frame = 0

Query: 33  VASIVSEELFKNLFLHKDDSVCPANGFYTYQAFIEATKRFSKFGTTGCLATRKREIAAFL 92
           ++ I+S   F ++  H++D+ CPA GFYTY AFI A K F  FGTTG  ATRK+E+AAF 
Sbjct: 86  LSGIISSSQFDDMLKHRNDAACPARGFYTYNAFITAAKSFPGFGTTGDTATRKKEVAAFF 145

Query: 93  AQISHETTGGWATAPDGPFAWGLCFKEEVNPQSNYCDASVVQWPCSPGKSYKGRGPIQLS 152
            Q SHETTGGWATAPDGP++WG CFK+E NP S+YC+ S   WPC+ GK Y GRGP+QLS
Sbjct: 146 GQTSHETTGGWATAPDGPYSWGYCFKQEQNPASDYCEPSAT-WPCASGKRYYGRGPMQLS 205

Query: 153 WNFNYGPAGKALGFDGLKNPEIVAEDSAIAFKTALWFWMTEQKPKPSCHDVMVGRYVPTE 212
           WN+NYG  G+A+G D L NP++VA D+ IAFK A+WFWMT Q PKPSCH V+ G++ P++
Sbjct: 206 WNYNYGLCGRAIGVDLLNNPDLVANDAVIAFKAAIWFWMTAQPPKPSCHAVIAGQWQPSD 265

Query: 213 ADREANRTAGYGLVTNIINGGLECGIANDARVNDRIGFFNRYAQLFNVDTGPNLDCQNQK 272
           ADR A R  GYG++TNIINGGLECG   D RV DRIGF+ RY  +F V+ G NLDC NQ+
Sbjct: 266 ADRAAGRLPGYGVITNIINGGLECGRGQDGRVADRIGFYQRYCNIFGVNPGGNLDCYNQR 325

Query: 273 PF 275
            F
Sbjct: 326 SF 326

BLAST of Lcy09g008280 vs. ExPASy Swiss-Prot
Match: Q9FRV1 (Basic endochitinase A OS=Secale cereale OX=4550 GN=rsca PE=1 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 5.1e-92
Identity = 150/242 (61.98%), Postives = 180/242 (74.38%), Query Frame = 0

Query: 33  VASIVSEELFKNLFLHKDDSVCPANGFYTYQAFIEATKRFSKFGTTGCLATRKREIAAFL 92
           V+SIVS  LF  + LH++D  C A GFYTY AF+ A   F  FGTTG   TRKRE+AAFL
Sbjct: 80  VSSIVSRALFDRMLLHRNDGACQAKGFYTYDAFVAAAGAFPGFGTTGSTDTRKREVAAFL 139

Query: 93  AQISHETTGGWATAPDGPFAWGLCFKEEVNPQSNYCDASVVQWPCSPGKSYKGRGPIQLS 152
           AQ SHETTGGWATAPDG FAWG CFK+E    SNYC  S  QWPC+PGKSY GRGPIQLS
Sbjct: 140 AQTSHETTGGWATAPDGAFAWGYCFKQERGATSNYCTPS-AQWPCAPGKSYYGRGPIQLS 199

Query: 153 WNFNYGPAGKALGFDGLKNPEIVAEDSAIAFKTALWFWMTEQKPKPSCHDVMVGRYVPTE 212
            N+NYGPAG+A+G D L+NP++VA D  ++FKTA+WFWMT Q PKPS H V+ G++ P+ 
Sbjct: 200 HNYNYGPAGRAIGVDLLRNPDLVATDPTVSFKTAMWFWMTAQAPKPSSHAVITGQWSPSG 259

Query: 213 ADREANRTAGYGLVTNIINGGLECGIANDARVNDRIGFFNRYAQLFNVDTGPNLDCQNQK 272
            DR A R  G+G++TNI+NGG+ECG   D+RV DRIGF+ RY  +  V  G NLDC NQ+
Sbjct: 260 TDRAAGRVPGFGVITNIVNGGIECGHGQDSRVADRIGFYKRYCDILRVGYGNNLDCYNQR 319

Query: 273 PF 275
           PF
Sbjct: 320 PF 320

BLAST of Lcy09g008280 vs. ExPASy TrEMBL
Match: A0A6J1JXK4 (chitinase 10-like OS=Cucurbita maxima OX=3661 GN=LOC111489215 PE=4 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 4.9e-146
Identity = 244/277 (88.09%), Postives = 257/277 (92.78%), Query Frame = 0

Query: 1   MASNFLFLSFILLLGIGLPST---EGHRRRREISPVASIVSEELFKNLFLHKDDSVCPAN 60
           MAS FLFLSFI LLG GLPST    GHRR RE S VAS++SEELFK LFLHKDDS CPAN
Sbjct: 1   MASTFLFLSFIFLLGTGLPSTIEAHGHRRWRERSRVASLISEELFKELFLHKDDSACPAN 60

Query: 61  GFYTYQAFIEATKRFSKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCF 120
           GFY+YQ+FIEATK+F KFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCF
Sbjct: 61  GFYSYQSFIEATKKFPKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCF 120

Query: 121 KEEVNPQSNYCDASVVQWPCSPGKSYKGRGPIQLSWNFNYGPAGKALGFDGLKNPEIVAE 180
           KEE++PQSNYCD SV QWPCSPGKSYKGRGPIQLSWN+NYGPAG+ALGFDGL+NPE VAE
Sbjct: 121 KEEISPQSNYCDDSVEQWPCSPGKSYKGRGPIQLSWNYNYGPAGEALGFDGLRNPERVAE 180

Query: 181 DSAIAFKTALWFWMTEQKPKPSCHDVMVGRYVPTEADREANRTAGYGLVTNIINGGLECG 240
           D   AFKTALWFWMTEQKPKPSCHDVMVGRY+PT+ADREANRTAGYGLVTNIINGGLECG
Sbjct: 181 DPTTAFKTALWFWMTEQKPKPSCHDVMVGRYIPTKADREANRTAGYGLVTNIINGGLECG 240

Query: 241 IANDARVNDRIGFFNRYAQLFNVDTGPNLDCQNQKPF 275
           IANDARVNDRIGFF RYA+LFNVDTGPNLDCQNQKPF
Sbjct: 241 IANDARVNDRIGFFQRYAKLFNVDTGPNLDCQNQKPF 277

BLAST of Lcy09g008280 vs. ExPASy TrEMBL
Match: A0A6J1FEL0 (chitinase 10-like OS=Cucurbita moschata OX=3662 GN=LOC111444802 PE=4 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 1.2e-144
Identity = 242/277 (87.36%), Postives = 256/277 (92.42%), Query Frame = 0

Query: 1   MASNFLFLSFILLLGIGLPST---EGHRRRREISPVASIVSEELFKNLFLHKDDSVCPAN 60
           MAS  LFLSFI LLG GLPST    GHRR RE S VAS++SEELFK LFLHKD+S CPAN
Sbjct: 1   MASTLLFLSFIFLLGPGLPSTIEAHGHRRWRERSRVASLISEELFKELFLHKDNSACPAN 60

Query: 61  GFYTYQAFIEATKRFSKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCF 120
           GFY+YQ+FIEATK+F KFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCF
Sbjct: 61  GFYSYQSFIEATKKFPKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCF 120

Query: 121 KEEVNPQSNYCDASVVQWPCSPGKSYKGRGPIQLSWNFNYGPAGKALGFDGLKNPEIVAE 180
           KEE++PQSNYCD SV QWPCSPGKSYKGRGPIQLSWN+NYGPAG+ALGFDGL+NPE VAE
Sbjct: 121 KEEISPQSNYCDDSVTQWPCSPGKSYKGRGPIQLSWNYNYGPAGEALGFDGLRNPERVAE 180

Query: 181 DSAIAFKTALWFWMTEQKPKPSCHDVMVGRYVPTEADREANRTAGYGLVTNIINGGLECG 240
           D   AFKTALWFWMTEQKPKPSCH+VMVGRY+PTEADREANRTAGYGLVTNIINGGLECG
Sbjct: 181 DPTTAFKTALWFWMTEQKPKPSCHNVMVGRYIPTEADREANRTAGYGLVTNIINGGLECG 240

Query: 241 IANDARVNDRIGFFNRYAQLFNVDTGPNLDCQNQKPF 275
           IANDARVNDRIGFF RYA+LFNVDTGPNLDCQNQKPF
Sbjct: 241 IANDARVNDRIGFFQRYAKLFNVDTGPNLDCQNQKPF 277

BLAST of Lcy09g008280 vs. ExPASy TrEMBL
Match: A0A1S3CQS5 (chitinase 10 OS=Cucumis melo OX=3656 GN=LOC103503712 PE=4 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 6.8e-140
Identity = 237/275 (86.18%), Postives = 250/275 (90.91%), Query Frame = 0

Query: 1   MASNFLFLSFILLLGIGLPS-TEGHRRRREISPVASIVSEELFKNLFLHKDDSVCPANGF 60
           M S  LFLSFILLL   LPS TE + RR +I  ++S++SEELFK LFLHKDDS CPANGF
Sbjct: 1   MTSKLLFLSFILLLLGSLPSMTESYGRREKICGISSLISEELFKTLFLHKDDSACPANGF 60

Query: 61  YTYQAFIEATKRFSKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKE 120
           Y+YQAFIEATKRF KFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKE
Sbjct: 61  YSYQAFIEATKRFPKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKE 120

Query: 121 EVNPQSNYCDASVVQWPCSPGKSYKGRGPIQLSWNFNYGPAGKALGFDGLKNPEIVAEDS 180
           E++PQSNYCD SV QWPC PGKSYKGRGPIQLSWNFNYGPAGKALGFDGLKNPE VAED 
Sbjct: 121 EISPQSNYCDDSVKQWPCYPGKSYKGRGPIQLSWNFNYGPAGKALGFDGLKNPERVAEDP 180

Query: 181 AIAFKTALWFWMTEQKPKPSCHDVMVGRYVPTEADREANRTAGYGLVTNIINGGLECGIA 240
             AFKTALWFWMTEQKPKPSCHDVMVG+YVPT+AD EANRTAGYGLVTNIINGGLECGIA
Sbjct: 181 TTAFKTALWFWMTEQKPKPSCHDVMVGQYVPTKADIEANRTAGYGLVTNIINGGLECGIA 240

Query: 241 NDARVNDRIGFFNRYAQLFNVDTGPNLDCQNQKPF 275
           NDARVNDRIG+F RYA+LFNVDTGPNLDCQNQK F
Sbjct: 241 NDARVNDRIGYFQRYAKLFNVDTGPNLDCQNQKHF 275

BLAST of Lcy09g008280 vs. ExPASy TrEMBL
Match: A0A5A7U2X7 (Chitinase 10 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00230 PE=4 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 1.5e-134
Identity = 222/253 (87.75%), Postives = 235/253 (92.89%), Query Frame = 0

Query: 22  EGHRRRREISPVASIVSEELFKNLFLHKDDSVCPANGFYTYQAFIEATKRFSKFGTTGCL 81
           E + RR +I  ++S++SEELFK LFLHKDDS CPANGFY+YQAFIEATKRF KFGTTGCL
Sbjct: 3   ESYGRREKICGISSLISEELFKTLFLHKDDSACPANGFYSYQAFIEATKRFPKFGTTGCL 62

Query: 82  ATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEVNPQSNYCDASVVQWPCSPGK 141
           ATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEE++PQSNYCD SV QWPC PGK
Sbjct: 63  ATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEISPQSNYCDDSVKQWPCYPGK 122

Query: 142 SYKGRGPIQLSWNFNYGPAGKALGFDGLKNPEIVAEDSAIAFKTALWFWMTEQKPKPSCH 201
           SYKGRGPIQLSWNFNYGPAGKALGFDGLKNPE VAED   AFKTALWFWMTEQKPKPSCH
Sbjct: 123 SYKGRGPIQLSWNFNYGPAGKALGFDGLKNPERVAEDPTTAFKTALWFWMTEQKPKPSCH 182

Query: 202 DVMVGRYVPTEADREANRTAGYGLVTNIINGGLECGIANDARVNDRIGFFNRYAQLFNVD 261
           DVMVG+YVPT+AD EANRTAGYGLVTNIINGGLECGIANDARVNDRIG+F RYA+LFNVD
Sbjct: 183 DVMVGQYVPTKADIEANRTAGYGLVTNIINGGLECGIANDARVNDRIGYFQRYAKLFNVD 242

Query: 262 TGPNLDCQNQKPF 275
           TGPNLDCQNQK F
Sbjct: 243 TGPNLDCQNQKHF 255

BLAST of Lcy09g008280 vs. ExPASy TrEMBL
Match: A0A6J1CYH8 (chitinase 10-like OS=Momordica charantia OX=3673 GN=LOC111015631 PE=4 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 7.6e-131
Identity = 217/251 (86.45%), Postives = 232/251 (92.43%), Query Frame = 0

Query: 24  HRRRREISPVASIVSEELFKNLFLHKDDSVCPANGFYTYQAFIEATKRFSKFGTTGCLAT 83
           +R RR+   V+S+VSEELFK +FLHKDDSVCPANGFY YQAFIEAT+ F KFG TGCLAT
Sbjct: 5   YRHRRDKGDVSSLVSEELFKTIFLHKDDSVCPANGFYPYQAFIEATRMFPKFGRTGCLAT 64

Query: 84  RKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEVNPQSNYCDASVVQWPCSPGKSY 143
           RKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEE++PQSNYCD S  QWPC PGKSY
Sbjct: 65  RKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEISPQSNYCDESDQQWPCYPGKSY 124

Query: 144 KGRGPIQLSWNFNYGPAGKALGFDGLKNPEIVAEDSAIAFKTALWFWMTEQKPKPSCHDV 203
           KGRGPIQLSWNFNYG AG+ALGFDGL+NPEIVAEDSAIAF+TALWFWMTE+ PKPSCHDV
Sbjct: 125 KGRGPIQLSWNFNYGLAGEALGFDGLRNPEIVAEDSAIAFQTALWFWMTERNPKPSCHDV 184

Query: 204 MVGRYVPTEADREANRTAGYGLVTNIINGGLECGIANDARVNDRIGFFNRYAQLFNVDTG 263
           MVGRYVPT+AD +ANRTAGYGLVTNIINGGLECGI +DARVNDRIGFF RYAQLFNVDTG
Sbjct: 185 MVGRYVPTKADIQANRTAGYGLVTNIINGGLECGIPDDARVNDRIGFFKRYAQLFNVDTG 244

Query: 264 PNLDCQNQKPF 275
           PNLDCQNQK F
Sbjct: 245 PNLDCQNQKSF 255

BLAST of Lcy09g008280 vs. NCBI nr
Match: XP_038875572.1 (chitinase 10-like [Benincasa hispida])

HSP 1 Score: 527.7 bits (1358), Expect = 5.9e-146
Identity = 245/275 (89.09%), Postives = 256/275 (93.09%), Query Frame = 0

Query: 1   MASNFLFLSFILLLGIGLPS-TEGHRRRREISPVASIVSEELFKNLFLHKDDSVCPANGF 60
           MAS FLFLSFI LLG GLPS TEGH RR +IS V+S+VSE+LFK LFLHKDDS CPANGF
Sbjct: 1   MASKFLFLSFIFLLGTGLPSMTEGHSRREKISRVSSLVSEKLFKTLFLHKDDSACPANGF 60

Query: 61  YTYQAFIEATKRFSKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKE 120
           Y+YQAFIEAT RF KFGTTGCLATRKRE+AAFLAQISHETTGGWATAPDGPFAWGLCFKE
Sbjct: 61  YSYQAFIEATTRFPKFGTTGCLATRKREVAAFLAQISHETTGGWATAPDGPFAWGLCFKE 120

Query: 121 EVNPQSNYCDASVVQWPCSPGKSYKGRGPIQLSWNFNYGPAGKALGFDGLKNPEIVAEDS 180
           E+NPQSNYCD SV QWPC PGKSYKGRGPIQLSWNFNYGPAGKALGFDGL+NPE VAED+
Sbjct: 121 EINPQSNYCDDSVKQWPCYPGKSYKGRGPIQLSWNFNYGPAGKALGFDGLRNPERVAEDA 180

Query: 181 AIAFKTALWFWMTEQKPKPSCHDVMVGRYVPTEADREANRTAGYGLVTNIINGGLECGIA 240
             AFKTALWFWMTEQKPKPSCHDVMVGRYVPT+AD EANRTAGYGLVTNIINGGLECGIA
Sbjct: 181 TTAFKTALWFWMTEQKPKPSCHDVMVGRYVPTKADIEANRTAGYGLVTNIINGGLECGIA 240

Query: 241 NDARVNDRIGFFNRYAQLFNVDTGPNLDCQNQKPF 275
           NDARVNDRIG+F RYAQLFNVDTGPNLDCQNQKPF
Sbjct: 241 NDARVNDRIGYFQRYAQLFNVDTGPNLDCQNQKPF 275

BLAST of Lcy09g008280 vs. NCBI nr
Match: XP_022993095.1 (chitinase 10-like [Cucurbita maxima])

HSP 1 Score: 526.9 bits (1356), Expect = 1.0e-145
Identity = 244/277 (88.09%), Postives = 257/277 (92.78%), Query Frame = 0

Query: 1   MASNFLFLSFILLLGIGLPST---EGHRRRREISPVASIVSEELFKNLFLHKDDSVCPAN 60
           MAS FLFLSFI LLG GLPST    GHRR RE S VAS++SEELFK LFLHKDDS CPAN
Sbjct: 1   MASTFLFLSFIFLLGTGLPSTIEAHGHRRWRERSRVASLISEELFKELFLHKDDSACPAN 60

Query: 61  GFYTYQAFIEATKRFSKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCF 120
           GFY+YQ+FIEATK+F KFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCF
Sbjct: 61  GFYSYQSFIEATKKFPKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCF 120

Query: 121 KEEVNPQSNYCDASVVQWPCSPGKSYKGRGPIQLSWNFNYGPAGKALGFDGLKNPEIVAE 180
           KEE++PQSNYCD SV QWPCSPGKSYKGRGPIQLSWN+NYGPAG+ALGFDGL+NPE VAE
Sbjct: 121 KEEISPQSNYCDDSVEQWPCSPGKSYKGRGPIQLSWNYNYGPAGEALGFDGLRNPERVAE 180

Query: 181 DSAIAFKTALWFWMTEQKPKPSCHDVMVGRYVPTEADREANRTAGYGLVTNIINGGLECG 240
           D   AFKTALWFWMTEQKPKPSCHDVMVGRY+PT+ADREANRTAGYGLVTNIINGGLECG
Sbjct: 181 DPTTAFKTALWFWMTEQKPKPSCHDVMVGRYIPTKADREANRTAGYGLVTNIINGGLECG 240

Query: 241 IANDARVNDRIGFFNRYAQLFNVDTGPNLDCQNQKPF 275
           IANDARVNDRIGFF RYA+LFNVDTGPNLDCQNQKPF
Sbjct: 241 IANDARVNDRIGFFQRYAKLFNVDTGPNLDCQNQKPF 277

BLAST of Lcy09g008280 vs. NCBI nr
Match: XP_023550747.1 (chitinase 10-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 523.9 bits (1348), Expect = 8.6e-145
Identity = 243/277 (87.73%), Postives = 256/277 (92.42%), Query Frame = 0

Query: 1   MASNFLFLSFILLLGIGLPST---EGHRRRREISPVASIVSEELFKNLFLHKDDSVCPAN 60
           MAS FLFLSFI LL  GLPST    GHRR RE S VAS++SEELFK LFLHKDDS CPAN
Sbjct: 1   MASTFLFLSFIFLLWTGLPSTIEAHGHRRWRERSRVASLISEELFKELFLHKDDSACPAN 60

Query: 61  GFYTYQAFIEATKRFSKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCF 120
           GFY+YQ+FIEATK+F KFGTTGCLAT+KREIAAFLAQISHETTGGWATAPDGPFAWGLCF
Sbjct: 61  GFYSYQSFIEATKKFPKFGTTGCLATQKREIAAFLAQISHETTGGWATAPDGPFAWGLCF 120

Query: 121 KEEVNPQSNYCDASVVQWPCSPGKSYKGRGPIQLSWNFNYGPAGKALGFDGLKNPEIVAE 180
           KEE++PQSNYCD SV QWPCSPGKSYKGRGPIQLSWN+NYGPAG+ALGFDGL+NPE VAE
Sbjct: 121 KEEISPQSNYCDDSVKQWPCSPGKSYKGRGPIQLSWNYNYGPAGEALGFDGLRNPERVAE 180

Query: 181 DSAIAFKTALWFWMTEQKPKPSCHDVMVGRYVPTEADREANRTAGYGLVTNIINGGLECG 240
           D   AFKTALWFWMTEQKPKPSCHDVMVGRY+PTEADREANRTAGYGLVTNIINGGLECG
Sbjct: 181 DPTTAFKTALWFWMTEQKPKPSCHDVMVGRYIPTEADREANRTAGYGLVTNIINGGLECG 240

Query: 241 IANDARVNDRIGFFNRYAQLFNVDTGPNLDCQNQKPF 275
           IANDARVNDRIGFF RYA+LFNVDTGPNLDCQNQKPF
Sbjct: 241 IANDARVNDRIGFFQRYAKLFNVDTGPNLDCQNQKPF 277

BLAST of Lcy09g008280 vs. NCBI nr
Match: KAG6579149.1 (Chitinase 10, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016667.1 Chitinase 10, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 522.3 bits (1344), Expect = 2.5e-144
Identity = 242/277 (87.36%), Postives = 255/277 (92.06%), Query Frame = 0

Query: 1   MASNFLFLSFILLLGIGLPST---EGHRRRREISPVASIVSEELFKNLFLHKDDSVCPAN 60
           MAS  LFLSFI LLG G PST    GHRR RE S VAS++SEELFK LFLHKDDS CPAN
Sbjct: 1   MASTLLFLSFIFLLGTGHPSTIEAHGHRRWRERSRVASLISEELFKELFLHKDDSACPAN 60

Query: 61  GFYTYQAFIEATKRFSKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCF 120
           GFY+YQ+FIEATK+F KFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCF
Sbjct: 61  GFYSYQSFIEATKKFPKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCF 120

Query: 121 KEEVNPQSNYCDASVVQWPCSPGKSYKGRGPIQLSWNFNYGPAGKALGFDGLKNPEIVAE 180
           KEE++PQSNYCD SV QWPCSPGKSYKGRGPIQLSWN+NYGPAG+ALGFDGL+NPE VAE
Sbjct: 121 KEEISPQSNYCDDSVTQWPCSPGKSYKGRGPIQLSWNYNYGPAGEALGFDGLRNPERVAE 180

Query: 181 DSAIAFKTALWFWMTEQKPKPSCHDVMVGRYVPTEADREANRTAGYGLVTNIINGGLECG 240
           D   AFKTALWFWMTEQKPKPSCH+VMVGRY+PTEADREANRTAGYGLVTNIINGGLECG
Sbjct: 181 DPTTAFKTALWFWMTEQKPKPSCHNVMVGRYIPTEADREANRTAGYGLVTNIINGGLECG 240

Query: 241 IANDARVNDRIGFFNRYAQLFNVDTGPNLDCQNQKPF 275
           IANDARVNDRIGFF RYA+LFNVDTGPNLDCQNQKPF
Sbjct: 241 IANDARVNDRIGFFQRYAKLFNVDTGPNLDCQNQKPF 277

BLAST of Lcy09g008280 vs. NCBI nr
Match: XP_022938622.1 (chitinase 10-like [Cucurbita moschata])

HSP 1 Score: 522.3 bits (1344), Expect = 2.5e-144
Identity = 242/277 (87.36%), Postives = 256/277 (92.42%), Query Frame = 0

Query: 1   MASNFLFLSFILLLGIGLPST---EGHRRRREISPVASIVSEELFKNLFLHKDDSVCPAN 60
           MAS  LFLSFI LLG GLPST    GHRR RE S VAS++SEELFK LFLHKD+S CPAN
Sbjct: 1   MASTLLFLSFIFLLGPGLPSTIEAHGHRRWRERSRVASLISEELFKELFLHKDNSACPAN 60

Query: 61  GFYTYQAFIEATKRFSKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCF 120
           GFY+YQ+FIEATK+F KFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCF
Sbjct: 61  GFYSYQSFIEATKKFPKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCF 120

Query: 121 KEEVNPQSNYCDASVVQWPCSPGKSYKGRGPIQLSWNFNYGPAGKALGFDGLKNPEIVAE 180
           KEE++PQSNYCD SV QWPCSPGKSYKGRGPIQLSWN+NYGPAG+ALGFDGL+NPE VAE
Sbjct: 121 KEEISPQSNYCDDSVTQWPCSPGKSYKGRGPIQLSWNYNYGPAGEALGFDGLRNPERVAE 180

Query: 181 DSAIAFKTALWFWMTEQKPKPSCHDVMVGRYVPTEADREANRTAGYGLVTNIINGGLECG 240
           D   AFKTALWFWMTEQKPKPSCH+VMVGRY+PTEADREANRTAGYGLVTNIINGGLECG
Sbjct: 181 DPTTAFKTALWFWMTEQKPKPSCHNVMVGRYIPTEADREANRTAGYGLVTNIINGGLECG 240

Query: 241 IANDARVNDRIGFFNRYAQLFNVDTGPNLDCQNQKPF 275
           IANDARVNDRIGFF RYA+LFNVDTGPNLDCQNQKPF
Sbjct: 241 IANDARVNDRIGFFQRYAKLFNVDTGPNLDCQNQKPF 277

BLAST of Lcy09g008280 vs. TAIR 10
Match: AT4G01700.1 (Chitinase family protein )

HSP 1 Score: 421.4 bits (1082), Expect = 5.6e-118
Identity = 187/268 (69.78%), Postives = 219/268 (81.72%), Query Frame = 0

Query: 8   LSFILLLGIGLPSTEGHRRRR-EISPVASIVSEELFKNLFLHKDDSVCPANGFYTYQAFI 67
           L FI  +   L  TE  +  + + +P+ S+V   L+  +F+HKD++ CPA GFY Y+AF+
Sbjct: 12  LLFIFSISSSLHETEARKHNKYKPAPIMSLVPRTLYDQIFIHKDNNACPAKGFYPYEAFV 71

Query: 68  EATKRFSKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEVNPQSN 127
           EAT+ F KFG+ G   TR+RE+AAFLAQISHETTGGWATAPDGP+AWGLCFKEEV+PQSN
Sbjct: 72  EATRSFPKFGSVGNFWTRRREVAAFLAQISHETTGGWATAPDGPYAWGLCFKEEVSPQSN 131

Query: 128 YCDASVVQWPCSPGKSYKGRGPIQLSWNFNYGPAGKALGFDGLKNPEIVAEDSAIAFKTA 187
           YCDAS   WPC  GKSYKGRGPIQLSWN+NYG AG+ALGFDGL+NPE+VA +S +AFKTA
Sbjct: 132 YCDASNKDWPCVSGKSYKGRGPIQLSWNYNYGQAGRALGFDGLQNPELVANNSVLAFKTA 191

Query: 188 LWFWMTEQKPKPSCHDVMVGRYVPTEADREANRTAGYGLVTNIINGGLECGIANDARVND 247
           LWFWMTEQ PKPSCH+VMV RY PT+ADR ANRT GYGLVTNIINGGLECGI  D RV D
Sbjct: 192 LWFWMTEQTPKPSCHNVMVNRYRPTKADRAANRTVGYGLVTNIINGGLECGIPGDGRVTD 251

Query: 248 RIGFFNRYAQLFNVDTGPNLDCQNQKPF 275
           R+G+F RYAQLF V TGPNLDC+NQ+PF
Sbjct: 252 RVGYFQRYAQLFKVTTGPNLDCENQRPF 279

BLAST of Lcy09g008280 vs. TAIR 10
Match: AT1G02360.1 (Chitinase family protein )

HSP 1 Score: 408.3 bits (1048), Expect = 4.9e-114
Identity = 178/271 (65.68%), Postives = 217/271 (80.07%), Query Frame = 0

Query: 4   NFLFLSFILLLGIGLPSTEGHRRRREISPVASIVSEELFKNLFLHKDDSVCPANGFYTYQ 63
           +FL L F L +   L S +      E + +  +V  +L+  +F+HKD++ CPANGFYTY+
Sbjct: 6   SFLLLCFFLSISYLLSSAQ-----TEATSIERLVPRDLYNKIFIHKDNTACPANGFYTYE 65

Query: 64  AFIEATKRFSKFGTTGCLATRKREIAAFLAQISHETTGGWATAPDGPFAWGLCFKEEVNP 123
           +F++AT+RF +FG+ G   T++ E+AAFLAQISHETTGGWATAPDGP+AWGLCFKEEV+P
Sbjct: 66  SFVQATRRFPRFGSVGSPVTQRLEVAAFLAQISHETTGGWATAPDGPYAWGLCFKEEVSP 125

Query: 124 QSNYCDASVVQWPCSPGKSYKGRGPIQLSWNFNYGPAGKALGFDGLKNPEIVAEDSAIAF 183
           QS YCD+S  QWPC P K+Y+GRGPIQLSWN+NYGPAG+ALGFDGL+NPE V+ +S IAF
Sbjct: 126 QSTYCDSSDTQWPCFPNKTYQGRGPIQLSWNYNYGPAGRALGFDGLRNPETVSNNSVIAF 185

Query: 184 KTALWFWMTEQKPKPSCHDVMVGRYVPTEADREANRTAGYGLVTNIINGGLECGIANDAR 243
           +TALWFWMT Q PKPSCHDVM+G+Y PT AD  ANRT G+GL TNIINGGLECGI  D R
Sbjct: 186 QTALWFWMTPQSPKPSCHDVMIGKYRPTAADLAANRTGGFGLTTNIINGGLECGIPGDGR 245

Query: 244 VNDRIGFFNRYAQLFNVDTGPNLDCQNQKPF 275
           VNDRIGFF RY  LF V TGPNLDC+NQ+P+
Sbjct: 246 VNDRIGFFQRYTGLFKVATGPNLDCENQRPY 271

BLAST of Lcy09g008280 vs. TAIR 10
Match: AT3G12500.1 (basic chitinase )

HSP 1 Score: 342.4 bits (877), Expect = 3.3e-94
Identity = 147/242 (60.74%), Postives = 182/242 (75.21%), Query Frame = 0

Query: 33  VASIVSEELFKNLFLHKDDSVCPANGFYTYQAFIEATKRFSKFGTTGCLATRKREIAAFL 92
           ++ I+S   F ++  H++D+ CPA GFYTY AFI A K F  FGTTG  ATRK+E+AAF 
Sbjct: 86  LSGIISSSQFDDMLKHRNDAACPARGFYTYNAFITAAKSFPGFGTTGDTATRKKEVAAFF 145

Query: 93  AQISHETTGGWATAPDGPFAWGLCFKEEVNPQSNYCDASVVQWPCSPGKSYKGRGPIQLS 152
            Q SHETTGGWATAPDGP++WG CFK+E NP S+YC+ S   WPC+ GK Y GRGP+QLS
Sbjct: 146 GQTSHETTGGWATAPDGPYSWGYCFKQEQNPASDYCEPSAT-WPCASGKRYYGRGPMQLS 205

Query: 153 WNFNYGPAGKALGFDGLKNPEIVAEDSAIAFKTALWFWMTEQKPKPSCHDVMVGRYVPTE 212
           WN+NYG  G+A+G D L NP++VA D+ IAFK A+WFWMT Q PKPSCH V+ G++ P++
Sbjct: 206 WNYNYGLCGRAIGVDLLNNPDLVANDAVIAFKAAIWFWMTAQPPKPSCHAVIAGQWQPSD 265

Query: 213 ADREANRTAGYGLVTNIINGGLECGIANDARVNDRIGFFNRYAQLFNVDTGPNLDCQNQK 272
           ADR A R  GYG++TNIINGGLECG   D RV DRIGF+ RY  +F V+ G NLDC NQ+
Sbjct: 266 ADRAAGRLPGYGVITNIINGGLECGRGQDGRVADRIGFYQRYCNIFGVNPGGNLDCYNQR 325

Query: 273 PF 275
            F
Sbjct: 326 SF 326

BLAST of Lcy09g008280 vs. TAIR 10
Match: AT1G05850.1 (Chitinase family protein )

HSP 1 Score: 198.0 bits (502), Expect = 1.0e-50
Identity = 96/242 (39.67%), Postives = 143/242 (59.09%), Query Frame = 0

Query: 42  FKNLFLHKDDSVCPANGFYTYQAFIEATKRFSK--FGTTGCLATRKREIAAFLAQISHET 101
           F+ LF  ++  +  A GF+ YQ+FI A   F    FGTTG     ++E+AAFL  ++ +T
Sbjct: 69  FEQLFSKRNTPIAHAVGFWDYQSFITAAALFEPLGFGTTGGKLMGQKEMAAFLGHVASKT 128

Query: 102 TGGWATAPDGPFAWGLCFKEEVNPQSNYCDAS-VVQWPCSPGKSYKGRGPIQLSWNFNYG 161
           + G+  A  GP AWGLC+  E++P  +YCD S   ++PCSPG  Y GRG + + WNFNYG
Sbjct: 129 SCGYGVATGGPLAWGLCYNREMSPMQSYCDESWKFKYPCSPGAEYYGRGALPIYWNFNYG 188

Query: 162 PAGKALGFDGLKNPEIVAEDSAIAFKTALWFWMTE-QKPKPSCHDVMVGRYVPTEADREA 221
            AG+AL  D L +PE + +++ +AF+ A+W WMT  ++ +PS HD+ VG + PT+ D  +
Sbjct: 189 AAGEALKADLLNHPEYIEQNATLAFQAAIWRWMTPIKRAQPSAHDIFVGNWKPTKNDTLS 248

Query: 222 NRTAGYGLVTNIINGGLECGIANDARVNDRIGFFNRYAQLFNV---DTGPN--LDCQNQK 275
            R   +G   N++ G   CG  +   +N+ I  +  +  L  +   D GPN  L C  QK
Sbjct: 249 KRGPTFGSTMNVLYGEYTCGQGSIDPMNNIISHYLYFLDLMGIGREDAGPNDELSCAEQK 308

BLAST of Lcy09g008280 vs. TAIR 10
Match: AT3G16920.1 (chitinase-like protein 2 )

HSP 1 Score: 191.8 bits (486), Expect = 7.2e-49
Identity = 97/244 (39.75%), Postives = 143/244 (58.61%), Query Frame = 0

Query: 42  FKNLFLHKDDSVCPANGFYTYQAFIEATKRFSK--FGTTGCLATRKREIAAFLAQISHET 101
           F+NLF  ++  V  A GF+ Y++FI A   +    FGT G      +E+AAFL  +  +T
Sbjct: 92  FENLFSKRNSPVAHAVGFWDYRSFITAAAEYQPLGFGTAGEKLQGMKEVAAFLGHVGSKT 151

Query: 102 TGGWATAPDGPFAWGLCFKEEVNPQSNYCDASV-VQWPCSPGKSYKGRGPIQLSWNFNYG 161
           + G+  A  GP AWGLC+ +E++P   YCD    + +PC+PG SY GRG + + WN+NYG
Sbjct: 152 SCGYGVATGGPLAWGLCYNKEMSPDQLYCDDYYKLTYPCTPGVSYHGRGALPVYWNYNYG 211

Query: 162 PAGKALGFDGLKNPEIVAEDSAIAFKTALWFWMT-EQKPKPSCHDVMVGRYVPTEADREA 221
             G+AL  D L +PE +  ++ +AF+ A+W WMT  +K  PS HDV VG++ PT+ D  A
Sbjct: 212 QTGEALKVDLLSHPEYLENNATLAFQAAIWRWMTPPKKHLPSAHDVFVGKWKPTKNDTAA 271

Query: 222 NRTAGYGLVTNIINGGLEC--GIANDARVNDRIGFFNRYAQLFNV---DTGPN--LDCQN 275
            RT G+G   N++ G   C  G  ND  +N+ +  +  Y  L  V   + GP+  L C +
Sbjct: 272 KRTPGFGATINVLYGDQICNSGFDND-EMNNIVSHYLYYLDLIGVGREEAGPHEKLSCAD 331

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5NB119.0e-11373.36Chitinase 10 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht10 PE=2 SV=1[more]
Q090231.4e-9461.98Endochitinase CH25 OS=Brassica napus OX=3708 PE=2 SV=1[more]
Q7DNA11.6e-9362.40Chitinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Cht2 PE=1 SV=1[more]
P191714.6e-9360.74Basic endochitinase B OS=Arabidopsis thaliana OX=3702 GN=CHI-B PE=1 SV=3[more]
Q9FRV15.1e-9261.98Basic endochitinase A OS=Secale cereale OX=4550 GN=rsca PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JXK44.9e-14688.09chitinase 10-like OS=Cucurbita maxima OX=3661 GN=LOC111489215 PE=4 SV=1[more]
A0A6J1FEL01.2e-14487.36chitinase 10-like OS=Cucurbita moschata OX=3662 GN=LOC111444802 PE=4 SV=1[more]
A0A1S3CQS56.8e-14086.18chitinase 10 OS=Cucumis melo OX=3656 GN=LOC103503712 PE=4 SV=1[more]
A0A5A7U2X71.5e-13487.75Chitinase 10 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00230 P... [more]
A0A6J1CYH87.6e-13186.45chitinase 10-like OS=Momordica charantia OX=3673 GN=LOC111015631 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_038875572.15.9e-14689.09chitinase 10-like [Benincasa hispida][more]
XP_022993095.11.0e-14588.09chitinase 10-like [Cucurbita maxima][more]
XP_023550747.18.6e-14587.73chitinase 10-like [Cucurbita pepo subsp. pepo][more]
KAG6579149.12.5e-14487.36Chitinase 10, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016667.1 Chit... [more]
XP_022938622.12.5e-14487.36chitinase 10-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT4G01700.15.6e-11869.78Chitinase family protein [more]
AT1G02360.14.9e-11465.68Chitinase family protein [more]
AT3G12500.13.3e-9460.74basic chitinase [more]
AT1G05850.11.0e-5039.67Chitinase family protein [more]
AT3G16920.17.2e-4939.75chitinase-like protein 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000726Glycoside hydrolase, family 19, catalyticPFAMPF00182Glyco_hydro_19coord: 36..268
e-value: 1.3E-109
score: 365.4
IPR000726Glycoside hydrolase, family 19, catalyticPROSITEPS00773CHITINASE_19_1coord: 54..76
IPR000726Glycoside hydrolase, family 19, catalyticPROSITEPS00774CHITINASE_19_2coord: 181..191
IPR016283Glycoside hydrolase, family 19PIRSFPIRSF001060Endochitinasecoord: 16..274
e-value: 9.7E-110
score: 364.6
NoneNo IPR availableGENE3D1.10.530.10coord: 40..274
e-value: 1.5E-114
score: 383.3
NoneNo IPR availableGENE3D3.30.20.10Endochitinase, domain 2coord: 119..177
e-value: 1.5E-114
score: 383.3
NoneNo IPR availablePANTHERPTHR22595CHITINASE-RELATEDcoord: 5..274
NoneNo IPR availablePANTHERPTHR22595:SF101OS05G0138200 PROTEINcoord: 5..274
NoneNo IPR availableCDDcd00325chitinase_GH19coord: 37..268
e-value: 7.59046E-114
score: 325.154
IPR023346Lysozyme-like domain superfamilySUPERFAMILY53955Lysozyme-likecoord: 33..274

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g008280.1Lcy09g008280.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0016998 cell wall macromolecule catabolic process
biological_process GO:0006032 chitin catabolic process
biological_process GO:0006952 defense response
molecular_function GO:0004568 chitinase activity
molecular_function GO:0008061 chitin binding