Lcy09g005390 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g005390
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionDolichyl-phosphate beta-glucosyltransferase
LocationChr09: 4713841 .. 4720786 (+)
RNA-Seq ExpressionLcy09g005390
SyntenyLcy09g005390
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCGGAGTAGCGCCAACCTATCGGCTCCCGCTGCAATTTTGAGCTACAGAATGGAGTATCCATGGACGATACTGTTCATTCTGATTGGTCTCGCTCTTAGTTTTGCAGCAGGTTTGATTTCTACTATCATCCTTGAAGCTTATAGGAGGAGATTCAATCACGAGTACGCCACTGCTGCATTCTTCATTGATTTGATAGCAATTTCATTTTTCAAAATGTCATATTTCTGTCTAGATGAGATTTTCTTTGTGCTTATTCATTTGATTTGTTTCAGGCACGTCGAAGCTCCTGCAATCTTCGAGGACCCAAACTCATTGAAACGGGTAAATTTTCTTTGAGCTTCGATTGGGAGTTTTATATGTCAGAGGCCATGTTTGATCGCTTGAAAGATTAATTAATACTTGTTAATGCCGACTCTCAAGCTTGTCAGTATTTCAATCACTCCAAGCTTGTCTTTGAAGAAATAGAACTTGTAGGCAGCTAGAGGTTTGGCCAAACAATCTGGAATTTGATCGTAGGATTATATGTACCTTAGCCATGGTTCTGTGTCGATAATTTTATCTCTTATCAAGTGGAGATGTAGCTCTAGATGTTTGGTTATTGCATGTAGACTCGGTTTACAGCCACGGCAGAAGCACTGGAGTTATGTCACCAAGAGATAGGTAATGAGTACCGATCAATGTGAAGTTCTTTGAGAATGGACTTGATCCAAGTTAATTCTACGACCATACTAGTTGGAGATCTATACTCTTAATTTTGCATTTGACATTGACCCAACACCTTGCTTCTTAGATTGCCATGAAACTAGATTTTCAACAAGAAAGACACAATTTATCCGATGGATCTTCTATCTTCCTTGCTGTGCCCAGTTGGCCTCTAGGAAGCTAGTCAAACGGAGTGTCTCACAAGATTTTATATGGAGGCCTTGATTTTGTGTTCTCCTCGAGTAGGGGAGAATTCTTTTGACCCTTGACCGACGATGAGTGAAGGACTTTGGAAGAATTGACTTAATTTGTTCACACTCCATGTTATGTTGGGTGTTGCGTAAGTTAACTATTGTTGAGCCCCTGTTGCATTTCTGGAAGTTCTTGGGTCACTCATCAATTCATATTCCTTATTTTTAATTCCCTACTCAGCACAATAGGAGTGGAGCAGGCCTTCACGGGATGTTTCGATACAAAGAGTTTGTCCAAATCCATAACGAACTTTTTTATCCTTGCTTTGCTATTTTCTTTGATCAATATATCATCAACATATACCTTCTTTGAAGTTTGAACACAAATATGGAAAAATTTGATATGAAATTATGAAGCCCAAACTTAATAGAAGTTGTTCTTAACCTTTTGAACCTTAAAGCTAGTTTTAGACAATACAAGGGCTTGTGTAGCTTGCATTCATGTTTAGCTTTCTTTAGGTCCACATAACCTTCTGGAAGCATATGCACATCTTCTTTAAACTATCCATCCAAAAAGGAGTTGTTTATATCCAATTTTTTTTTAATGAAATTTTTTTTGTTTCCTGATGAAAAGAGATATTTGAATTCTTCTGAGTCACAGCCTCACTTATCCCAAAAGGTCCCAGCCTCTTGTTGCTGCTATGGTAATAGGTAGGGATGATTTTGATGGTTAAAGATTTAACCATAGGGATATAGGTTTCATTGAAGTCTATTCTAGGATTTTGATGGAATCCTTTACCGACCAACCTGACAGTTAACTAGTGTAGGGATTATCTGAGTTTAGTTTCACCTTGAACACCAATCTTTTCCCAGTGATCTTTAATTCTGATGAGAGGGGAAATAGGTTCCAAATTTTTTTAAGCAAAGCAGTAAATTCTTCCTCCATAGCCCTTTCCAATGGGCAGAGACTAAAGCTCCTTGTTGATTATGGATTGTATTGTTCCACTGAATTTTAAGTTTTTGTTTTGGACAATATGAGGTCAGTACCTTTTGGGGATAAGATCCCCATCTCTTGTTCCAGTAATCAGAGGTGCCTTTGGTCTTGTTCAATTCTGTTTGAAGGGTACCCACAGTAATCTTTAAGTCCTTTTCAAAGCTCTTCTGAAGCCTTAAAATGAAAACTTGTGTTGCAACTTCAAAGTAAGTAACTTGTACAACCCACCAGTGAGACGTTGATCATTAGCGACCCACTCATATGTCATACTCAGAATTACGAACAGCCTGATTTGAGGTTCCTTCAGAAGTTAATTGTGGGGAGCATTTGGTTCCTAGGATGTAGCCTTCTAATATTTTTCCTCAAATGATAAGAATAACCATTGTTCCCCATAGAAGATAGTCAAAAAGGAATGATTCTGATTCAAATTGCTGCCGCAGGATGTCTTGTTGTTGATTTTAGTTGCTCCGTGATTTATGAGATTTGAGGAATCTGATCTTGCCTGATTGAGAAACAGATAGACATTGCACAGGCCTGTGGAAGGGTAAAGATGATAAGGGAAGGAAAAATTCTTGAATTTTTTTCATTGAATTATTCAGATAATGAGTACAGCTATATATAGGCAAGAAGTCTTACAGCTTTCCAGCTCTAACAGAAATAATGAGTCTTTTTTTCAAAAAAAAAAAAGAAAAAAAAAAAGAAAAAACAGAAATAATCTCTCACAAATCTTCTAAAGCAACCTTAGCTGACCAGACAAACAAAACACTAGTACAATTTACAACTTCTTAACAACCACTGCCAGCACCTAATGGCCACACCCCGTAAGCCAAGAGTGGGTGCAAACCAGACATCGAGCTTGATTTTTAATTGGTGAAATTTTAAGAGGAGAGAGATTTGGTTAGGATGTTGCAATCTGAGTTGAGGAAGGCAGAGGTTGAACACGCTAAGAACTAAATCACATACAAAGTAAATGTCATGTTCATATTTAGTCTGAGACTCTGAGAGCGTAAAATTGGTTTGAAACTAAAACTCTTTTCTTTTATTTGAATCAAGTAGTCTATAATGGGAAAGAAAAAAGATTCTTTAAAGTAGAGTTATTATATTGTACTCCCATTGGAATAAATTGATGGATTCTTTTTTTTTTTAATGATAAACTAGACTTTCACCCAGAAAAATGAAAGAATATAGAAGCGCATATAAGAGAAAAAGAAGTCCAAAAAACGGAGTGTATGTAGAAACTAGCCAAACAAGAAGAAACTCCAAAATTGATGGAAATTATAAATTACATCATGTTTATGTTTGTAATATCCGATCAAAATTGATACCAAGTATTGTGTAGAATGCATGAGAGCAAGTTTCTTGAATGTTTTTCAGGTTCCTTGCCCTTCAATTTTCGATCCAGCTGAGAAGTATCTGTCCTTGATCGTTCCTGCTTTCAATGAAGAGTACAGGCTTCATGGAGCTCTCGAGGAAACCATTAAGTGAGTTGAAACATTACTTCAAATCATTTCACTTGTTCCATATAAGTATTTTTACTCGTCTTTTATACCCACTAGCTCTCGTTTGACCTTGATTTTCTCTGCATACCAACCCTATCTACACAGATTTTTCCTAATTCTTGGATTTTTCGATTTTGTCCTCATAGTCTCTACTTAGCTAGATACGACTGGCAAAGTAAGACCCCGAGAGCATGTTTCTCAACTTTTAAAGGCCACAATCATTTCCTAATTAATGGAAAATATTAGTAGGTTTAGGCTACGAGACAAAGTATAATCTCATTTGTGCTTTGCTTTTTAGCTATCTTCAACAACGTGCAGCAAAGGACAAGACATTTTCCTATGAGGTAATTTTTTTCATTTATTCCTTTTATTCTTGTTCCTTTTTTTATTTTATTCTTGTTCCTGATCAGGCATTGATCGGGACAGCTCTAAGAAAATTTGTATTGATGGTTTTTTGCATATCTATATGACTATAACTTCATATGCCAATATTTTCTTGCTTGTGATACCTTGATATATTCATTTCCCTTTTTCCATTAGCTCATGAAGTTGGAAATTTCATCTGAATTTATCCACAGAAGTAGATTAAAAAGTATATATTGGCATTCTTCCACACTTAGAGAATGATCTAATTTTTTATTTCTCTTTATGATTTTCTATGTCCGTATTCAAAGACATTCTGGCAATAAGACATGAAAATTTCCCCTGATTTTTTTGAACGATGAATGAGCTAGGCCTGCTCGAGTAATGAAATGAGCCCCTCGCTTTTTCTTCTCAATAAATTTTCATGACCACAAGTTGCATTCGGCTACTGAACTGTGTGGATGATGCCTTCCACTTCTGAATTGTGAATGTGGTTAAGACTTGTTGCTTTGCTTCAGTCTATTTTAATGATGAAAACTTGTACTTGTTCTAATTCTTCCAGAACACAAACTGTTTAAAACACCAAATATTAAATCAATTAATGCTATTTCAAATTTATTTAGACTATAGTTTCAACCAATGAATTATAAATTTGTGAAGCCAAATTTATGAAATAAAATTTCATTTGTTTAGAAACTCATTACTTGTTATTATTAATTTTAATGAACTTGTAATTAGAAAGAGAGTATAGTTCTTTTCAAAAAAAAAAAAAAAAAAAAGAGAGTATAGTTATGATTTAAATTATTGAGAGTAATTAATTGATTTTGTCATGCTTTGTAAATTTATGATTTGTGAAGGAGATAATGGCCTAAAACTTAGTTTGAGCTTTTCTATTATATACTAGACCGTAGATTATTGATCTGGTAGAATACTAAACTACGTAGAAACTTCGTTTTAACAACAAAAATAGAAACTTATTTAAAAGTTCTTTTTGCTCATTTGTCTCCCATTTCCGTCCACCTGAGATAAAAGAATGGGAAGGTATGGTGCATATGATGCATGAAGCTGAATTAATCATAGGGGAACATGTTTCGCACGCTTTGCCTTTTGTGCTGAACATTTAATCATCCGACTTGAACAGGTGATAATAATTGATGATGGGAGTGTCGATGGAACAAAAGGAGTAGCTTTCACTTTTGTTAGGAAGTACTCCGTAGAAAACGTGAGGGTTATACTTCTTGGAAAGAATCATGGAAAAGGGGAAGCTATTAGAAAGGTGAGTTTGGACTTCGCACTTTATTTGTAATACTTGTATGATTATTATTCTCTTATACCATTTTGTGATGGAAAGATGGTGAGACTCCTTACTTTCTAAAAATCATTTTCAAGCAGGGAATGCTTCACTCACGTGGTGAGCTACTTCTAATGCTGGATGCTGATGGAGCAACCAAAATTACTGACCTAGAAAAACTTGAAAACCAGGTTTCGACAAACCCAATCTATTTTCAAGTGCAATTTTTTCCAATGAAAGCCAAGGCCCCAACTTCTAAAAATGTGAAATAGTTATTCAAGACGAAAATTTTCTCCCCCAGATCCATGCAGTGCCAATAGTTAAGAAGGAAGCTTCAAGAGATCCGAGTGTTAGAATATCTGATATTCCAATCACCGCATTTGGTTCTCGCGCTCATCTTGAGGAGAAGGCTCTGGCTACAGTTTAGTATAGCTCTTGCAGTTAACTCGCTAACTAATTTGAGCTTTATGTTTTATTTGATGGGTTCTTTCACTTTTCAGAGAAAGTGGTATCGCAATTTCCTGATGAAGGGCTTTCATCTTGTGGTTCTTCTAACTGCAGGTCCTGGAATACGGGATACACAGGTGATTCTTTTTTCAGTTTGCAACGCATGCACATGGTCCTTCATGTATATGTGTGCCATTATCTCTGTGCTTATGCTGTGCACTAATACAGATGTAGTAAGGATTCATGCTAACTATAGCCTGAAGCTGCTAAACTTCATTTTTCAATATAGGATAAATTACATCCTTATTGACGTTGAATTTCCTTTTATCAAAATGGTATTTGCTTTAAAAAAATTTATATTTTAATTGATGTATCATTAATTTAAGAAAAGTGACGTGTCACCGTATCCGAGTCGTATCTTACCCATATTTATACTTCTTGAATCCTTACTTTCTATTACCGTGATTATACACAGTGTGGTTTTAAGATGTTCACGAGATCTGCTGCCAGGAAGCTTTTTACGAACATTCGTTTGAAGAGGTAAGATCCCTCCCCAGGATTTTCTCTCTTCAAGTCACAGCAGCTTATTTGAGGGTTTAACTCGGACTATCATGTCATACGGAGTAGATCCCGCCCTCTTTTAGGTGCATGCAACCACACCAGAAATAATATGGTAAATCAATGAATACATAGTGGAATTAATTGAATCCAATCTGAGAGCAAAACCATAACAATTGAAGTTTGTTGACTTGTTTTGTTTTTTCATTCTCTTTCTTAGTCCTGCCACTTGTTGTCGAAAAATCCTTTTCAACAAGAGATCATCTCTGATCTGTGCTTGCATGAAATTGCTGATTCTGATCAATGGATCAAATAAGCAAGGACTGACCGACTGAGCATTCACTTCCTGCAGGTGGTGCTTTGATGTTGAGTTGGTTTACCTCTGCAAATATTTTGGCATTTCTATGATCGAAGTATCTGTGAACTGGTCTGAAATACCCGGATCCAAGCTAAGTCCACGGAGCATTTTAAACATGCTTTGGGAGCTCGCACTCATGTCTATGGGATACAGAACCGGAATTTGGAGAATTTCAACCTGAGCTTCCCACTGAATTTCTCAGATCCCCCCCCCCCCCCAAAAAAAAAAAAAAAGTGAAAGATGATCAGTTCACCAATGCAGTACATACTACACCCGCAATAGTTCGTGGTGTCCAAAATTTATAGCTGACCATTCCCCTTCTAATAATTCTGTAAAATTTTGTTTGCCAATGTGATTATTCTTTTCTTTTTTTCTTTTTCCTTATTATTTTTCAAATTCAATATAACATCTCATCTCAGACGCATTTGCTTTGATACATGTGGGTACGTTGTTCAATTGCAAAAGCTTAGTGGGATTGCATCTCTGACGATATCTTTGAGATACTCTTGTTTATATTTTGGGCAGGGCGGCAATATACAAAC

mRNA sequence

GCGGAGTAGCGCCAACCTATCGGCTCCCGCTGCAATTTTGAGCTACAGAATGGAGTATCCATGGACGATACTGTTCATTCTGATTGGTCTCGCTCTTAGTTTTGCAGCAGGTTTGATTTCTACTATCATCCTTGAAGCTTATAGGAGGAGATTCAATCACGAGCACGTCGAAGCTCCTGCAATCTTCGAGGACCCAAACTCATTGAAACGGGTTCCTTGCCCTTCAATTTTCGATCCAGCTGAGAAGTATCTGTCCTTGATCGTTCCTGCTTTCAATGAAGAGTACAGGCTTCATGGAGCTCTCGAGGAAACCATTAACTATCTTCAACAACGTGCAGCAAAGGACAAGACATTTTCCTATGAGGTGATAATAATTGATGATGGGAGTGTCGATGGAACAAAAGGAGTAGCTTTCACTTTTGTTAGGAAGTACTCCGTAGAAAACGTGAGGGTTATACTTCTTGGAAAGAATCATGGAAAAGGGGAAGCTATTAGAAAGGGAATGCTTCACTCACGTGGTGAGCTACTTCTAATGCTGGATGCTGATGGAGCAACCAAAATTACTGACCTAGAAAAACTTGAAAACCAGATCCATGCAGTGCCAATAGTTAAGAAGGAAGCTTCAAGAGATCCGAGTGTTAGAATATCTGATATTCCAATCACCGCATTTGGTTCTCGCGCTCATCTTGAGGAGAAGGCTCTGGCTACAAGAAAGTGGTATCGCAATTTCCTGATGAAGGGCTTTCATCTTGTGGTTCTTCTAACTGCAGGTCCTGGAATACGGGATACACAGTGTGGTTTTAAGATGTTCACGAGATCTGCTGCCAGGAAGCTTTTTACGAACATTCGTTTGAAGAGGTGGTGCTTTGATGTTGAGTTGGTTTACCTCTGCAAATATTTTGGCATTTCTATGATCGAAGTATCTGTGAACTGGTCTGAAATACCCGGATCCAAGCTAAGTCCACGGAGCATTTTAAACATGCTTTGGGAGCTCGCACTCATGTCTATGGGATACAGAACCGGAATTTGGAGAATTTCAACCTGAGCTTCCCACTGAATTTCTCAGATCCCCCCCCCCCCCCAAAAAAAAAAAAAAAGTGAAAGATGATCAGTTCACCAATGCAGTACATACTACACCCGCAATAGTTCGTGGTGTCCAAAATTTATAGCTGACCATTCCCCTTCTAATAATTCTGTAAAATTTTGTTTGCCAATGTGATTATTCTTTTCTTTTTTTCTTTTTCCTTATTATTTTTCAAATTCAATATAACATCTCATCTCAGACGCATTTGCTTTGATACATGTGGGTACGTTGTTCAATTGCAAAAGCTTAGTGGGATTGCATCTCTGACGATATCTTTGAGATACTCTTGTTTATATTTTGGGCAGGGCGGCAATATACAAAC

Coding sequence (CDS)

ATGGAGTATCCATGGACGATACTGTTCATTCTGATTGGTCTCGCTCTTAGTTTTGCAGCAGGTTTGATTTCTACTATCATCCTTGAAGCTTATAGGAGGAGATTCAATCACGAGCACGTCGAAGCTCCTGCAATCTTCGAGGACCCAAACTCATTGAAACGGGTTCCTTGCCCTTCAATTTTCGATCCAGCTGAGAAGTATCTGTCCTTGATCGTTCCTGCTTTCAATGAAGAGTACAGGCTTCATGGAGCTCTCGAGGAAACCATTAACTATCTTCAACAACGTGCAGCAAAGGACAAGACATTTTCCTATGAGGTGATAATAATTGATGATGGGAGTGTCGATGGAACAAAAGGAGTAGCTTTCACTTTTGTTAGGAAGTACTCCGTAGAAAACGTGAGGGTTATACTTCTTGGAAAGAATCATGGAAAAGGGGAAGCTATTAGAAAGGGAATGCTTCACTCACGTGGTGAGCTACTTCTAATGCTGGATGCTGATGGAGCAACCAAAATTACTGACCTAGAAAAACTTGAAAACCAGATCCATGCAGTGCCAATAGTTAAGAAGGAAGCTTCAAGAGATCCGAGTGTTAGAATATCTGATATTCCAATCACCGCATTTGGTTCTCGCGCTCATCTTGAGGAGAAGGCTCTGGCTACAAGAAAGTGGTATCGCAATTTCCTGATGAAGGGCTTTCATCTTGTGGTTCTTCTAACTGCAGGTCCTGGAATACGGGATACACAGTGTGGTTTTAAGATGTTCACGAGATCTGCTGCCAGGAAGCTTTTTACGAACATTCGTTTGAAGAGGTGGTGCTTTGATGTTGAGTTGGTTTACCTCTGCAAATATTTTGGCATTTCTATGATCGAAGTATCTGTGAACTGGTCTGAAATACCCGGATCCAAGCTAAGTCCACGGAGCATTTTAAACATGCTTTGGGAGCTCGCACTCATGTCTATGGGATACAGAACCGGAATTTGGAGAATTTCAACCTGA

Protein sequence

MEYPWTILFILIGLALSFAAGLISTIILEAYRRRFNHEHVEAPAIFEDPNSLKRVPCPSIFDPAEKYLSLIVPAFNEEYRLHGALEETINYLQQRAAKDKTFSYEVIIIDDGSVDGTKGVAFTFVRKYSVENVRVILLGKNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQIHAVPIVKKEASRDPSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAGPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMIEVSVNWSEIPGSKLSPRSILNMLWELALMSMGYRTGIWRIST
Homology
BLAST of Lcy09g005390 vs. ExPASy Swiss-Prot
Match: Q9Y673 (Dolichyl-phosphate beta-glucosyltransferase OS=Homo sapiens OX=9606 GN=ALG5 PE=1 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.8e-67
Identity = 143/329 (43.47%), Postives = 205/329 (62.31%), Query Frame = 0

Query: 4   PWTILFILIGLALSFAAGLISTII--LEAYRRRFNHEHVEAPAIFEDPNSLKRVPCPSIF 63
           P  +   ++G AL+ AA ++ +I+    A +    H H E    F +    K    PSI+
Sbjct: 3   PLLLQLAVLGAALAAAALVLISIVAFTTATKMPALHRH-EEEKFFLNAKGQKET-LPSIW 62

Query: 64  DPAEKYLSLIVPAFNEEYRLHGALEETINYLQQRAAKDKTFSYEVIIIDDGSVDGTKGVA 123
           D   K LS++VP++NEE RL   ++E ++YL++R  +D  F+YEVI++DDGS D T  VA
Sbjct: 63  DSPTKQLSVVVPSYNEEKRLPVMMDEALSYLEKRQKRDPAFTYEVIVVDDGSKDQTSKVA 122

Query: 124 FTFVRKYSVENVRVILLGKNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQI 183
           F + +KY  + VRVI L KN GKG AIR G+  SRGE +LM DADGATK  D+EKLE  +
Sbjct: 123 FKYCQKYGSDKVRVITLVKNRGKGGAIRMGIFSSRGEKILMADADGATKFPDVEKLEKGL 182

Query: 184 HAVPIVKKEASRDPSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAG 243
           + +     + +             A GSRAHLE++++A R ++R  LM GFH +V     
Sbjct: 183 NDLQPWPNQMA------------IACGSRAHLEKESIAQRSYFRTLLMYGFHFLVWFLCV 242

Query: 244 PGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMIEVSVNWSEIPGS 303
            GIRDTQCGFK+FTR AA + F+++ ++RW FDVEL+Y+ ++F I + E++VNW+EI GS
Sbjct: 243 KGIRDTQCGFKLFTREAASRTFSSLHVERWAFDVELLYIAQFFKIPIAEIAVNWTEIEGS 302

Query: 304 KLSP-RSILNMLWELALMSMGYRTGIWRI 330
           KL P  S L M  +L  + + Y TG WR+
Sbjct: 303 KLVPFWSWLQMGKDLLFIRLRYLTGAWRL 317

BLAST of Lcy09g005390 vs. ExPASy Swiss-Prot
Match: Q9VLQ1 (Dolichyl-phosphate beta-glucosyltransferase OS=Drosophila melanogaster OX=7227 GN=wol PE=1 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 2.6e-66
Identity = 150/332 (45.18%), Postives = 206/332 (62.05%), Query Frame = 0

Query: 5   WTIL----FILIGLALSFAAGLISTIILEAYRRRFNHEHVEAPAIFEDPNSLKRVPCPSI 64
           WT L    F L+   L+ AA  I+ ++L   +   N +  +    F DP+++K V  PS+
Sbjct: 2   WTCLCQLCFYLLS-TLAVAALSIAALVLYKTKPYPNIKRHKDEETFLDPHTIKTVTFPSL 61

Query: 65  FDPAEKYLSLIVPAFNEEYRLHGALEETINYLQQRAAKDKTFSYEVIIIDDGSVDGTKGV 124
            D     LS+IVPA+NEE RL   L+E + +L+Q++A    F+YEVI++ DGS D T  V
Sbjct: 62  EDSPSLELSVIVPAYNEEQRLPSMLDECLAFLEQKSAGTPNFTYEVIVVSDGSQDATVSV 121

Query: 125 AFTFVRKYSVENVRVILLGKNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 184
           A  + +K+  E VRV+ L +N GKG A+R GML +RG  LL  DADGATK  D +KLE  
Sbjct: 122 ALGYSKKHGAEKVRVLELIENRGKGGAVRMGMLSARGRNLLFADADGATKFPDYDKLE-- 181

Query: 185 IHAVPIVKKEASRDPSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 244
                +  K+ +  P  R   I   A GSRAHLE  A+ATR ++R  LM GFH +V L A
Sbjct: 182 -----VALKQLA--PEWRDDGI---AIGSRAHLENDAIATRSFFRTILMHGFHFLVWLFA 241

Query: 245 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMIEVSVNWSEIPG 304
              IRDTQCGFK+FTR+ ARKLFT++ ++RW FDVEL+YL +   + M EV+V W+EI G
Sbjct: 242 VRSIRDTQCGFKLFTRTTARKLFTSLHVERWAFDVELLYLAENLKLPMSEVAVRWTEIDG 301

Query: 305 SKLSP-RSILNMLWELALMSMGYRTGIWRIST 332
           SKL+P  S L M  +L ++ + Y  G WRI++
Sbjct: 302 SKLTPFWSWLQMGRDLFMIWVRYLVGAWRIAS 320

BLAST of Lcy09g005390 vs. ExPASy Swiss-Prot
Match: Q9DB25 (Dolichyl-phosphate beta-glucosyltransferase OS=Mus musculus OX=10090 GN=Alg5 PE=1 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 7.6e-66
Identity = 141/329 (42.86%), Postives = 202/329 (61.40%), Query Frame = 0

Query: 6   TILFILIGLALSFAAGLISTIILEAYRRRFN----HEHVEAPAIFEDPNSLKRVPCPSIF 65
           T+L  L+GL ++ AA  +  + + A+         ++H E    F +    K    PSI+
Sbjct: 3   TLLLQLLGLGVALAAAALILVSIVAFITATKMPPCYQH-EEEKFFLNAKGQKEA-LPSIW 62

Query: 66  DPAEKYLSLIVPAFNEEYRLHGALEETINYLQQRAAKDKTFSYEVIIIDDGSVDGTKGVA 125
           D   K LS++VP++NEE RL   ++E +NYL++R   D TF+YEVI++DDGS D T  VA
Sbjct: 63  DSPTKQLSVVVPSYNEEKRLPVMMDEALNYLEKRQKHDCTFTYEVIVVDDGSEDQTSKVA 122

Query: 126 FTFVRKYSVENVRVILLGKNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQI 185
             + +KY  + VRVI L +N GKG A+R G+  SRGE +LM DADGATK  D+EKLE  +
Sbjct: 123 LKYCQKYGSDKVRVITLVRNRGKGGAVRMGVFSSRGEKILMADADGATKFPDVEKLEKGL 182

Query: 186 HAVPIVKKEASRDPSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTAG 245
             +    ++ +             A GSRAHLE++++A R ++R FLM GFH +V     
Sbjct: 183 SDLQPWPEQMA------------IACGSRAHLEKESIAQRSYFRTFLMYGFHFLVWFLCV 242

Query: 246 PGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMIEVSVNWSEIPGS 305
            GIRDTQCGFK+ TR AA + F+++ ++RW FDVEL+Y+ +   I + EV+VNW+EI GS
Sbjct: 243 KGIRDTQCGFKLLTREAAARTFSSLHIERWAFDVELLYIAQCLQIPIAEVAVNWTEIEGS 302

Query: 306 KLSP-RSILNMLWELALMSMGYRTGIWRI 330
           KL P  S L M  +L  + + Y TG WR+
Sbjct: 303 KLVPFWSWLQMGKDLLFIRLRYLTGAWRL 317

BLAST of Lcy09g005390 vs. ExPASy Swiss-Prot
Match: A2DZE8 (Dolichyl-phosphate beta-glucosyltransferase ALG5A OS=Trichomonas vaginalis OX=5722 GN=ALG5A PE=1 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 7.4e-61
Identity = 122/281 (43.42%), Postives = 177/281 (62.99%), Query Frame = 0

Query: 49  PNSLKRVPCPSIFDPAEKYLSLIVPAFNEEYRLHGALEETINYLQQRAAKDKTFSYEVII 108
           PN  ++VP P+IFDPA  YLSL+VPA+NEE RL   L+ET+NYL+ R  KDK+F++E++I
Sbjct: 56  PNGNEKVPFPTIFDPASVYLSLVVPAYNEEKRLPKMLDETLNYLKSREEKDKSFTWEIVI 115

Query: 109 IDDGSVDGTKGVAFTFVRKYSVENVRVILLGKNHGKGEAIRKGMLHSRGELLLMLDADGA 168
           ++DGS D TK V   + ++Y   N+ ++    N GKG AI+ G LH RGEL+LMLDADGA
Sbjct: 116 VNDGSKDKTKEVVLNYAKEY--PNIFLLNQPVNMGKGAAIQAGCLHVRGELVLMLDADGA 175

Query: 169 TKITDLEKLENQIHAVPIVKKEASRDPSVRISDIPITAFGSRAHLEEKALATRKWYRNFL 228
           TKI D E LE +I ++     +A                GSRA  E+   A R   R FL
Sbjct: 176 TKIDDFEVLEKEIKSLMKTTNQA-------------IVIGSRAQNEK---AKRTPLRKFL 235

Query: 229 MKGFHLVVLLTAGPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISM 288
             G H +++L+   GIRDTQCGFK+FTR + + +F N  ++RWC D E++ + +  G+ +
Sbjct: 236 SIGMHTLIVLSGVHGIRDTQCGFKLFTRESCKMIFMNQHVQRWCCDPEILVIARRLGMKI 295

Query: 289 IEVSVNWSEIPGSKLSPRSILNMLWELALMSMGYRTGIWRI 330
            E+ V W+EI GSK+    ++ M  +L  +++ +R G W+I
Sbjct: 296 SELPVEWNEIDGSKMKISGMIKMATDLIKIAIFHRVGAWKI 318

BLAST of Lcy09g005390 vs. ExPASy Swiss-Prot
Match: A2ELE6 (Dolichyl-phosphate beta-glucosyltransferase ALG5C OS=Trichomonas vaginalis OX=5722 GN=ALG5C PE=1 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 4.8e-60
Identity = 114/281 (40.57%), Postives = 178/281 (63.35%), Query Frame = 0

Query: 49  PNSLKRVPCPSIFDPAEKYLSLIVPAFNEEYRLHGALEETINYLQQRAAKDKTFSYEVII 108
           P+  +++P P++F P+E Y + +VPA+NE  R+   L+ET+ YL++RA+++  F++E+I+
Sbjct: 59  PSPKEKIPFPTVFSPSEVYTTFVVPAYNESKRITPMLDETVAYLERRASENPEFTWEIIV 118

Query: 109 IDDGSVDGTKGVAFTFVRKYSVENVRVILLGKNHGKGEAIRKGMLHSRGELLLMLDADGA 168
           ++DGS D T  +   +  K+    +R++   KN GKG A++ G LHSRGEL+LM+DADGA
Sbjct: 119 VNDGSKDNTAEIVTNYAFKH--PQIRLLNQPKNMGKGAAVQAGCLHSRGELILMVDADGA 178

Query: 169 TKITDLEKLENQIHAVPIVKKEASRDPSVRISDIPITAFGSRAHLEEKALATRKWYRNFL 228
           TKI + E+LE +I ++  + KEA                GSRAHLE    A R   R FL
Sbjct: 179 TKIDEFEELEKKIKSLTTINKEA-------------IVVGSRAHLEGAEKANRTPLRKFL 238

Query: 229 MKGFHLVVLLTAGPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISM 288
             GFH+++ +    GI+DTQCGFK+FTR AAR LF N  ++RWCFD EL+ + +   + +
Sbjct: 239 GLGFHMLITIAGVHGIKDTQCGFKLFTREAARWLFPNQHVQRWCFDPELLVIAQSRQMEV 298

Query: 289 IEVSVNWSEIPGSKLSPRSILNMLWELALMSMGYRTGIWRI 330
            EV V W+EI  SK+    ++ M  +L  +++ +R G+W +
Sbjct: 299 AEVPVEWNEIGDSKMKISGMIKMAIDLVQIAIYFRAGLWTV 324

BLAST of Lcy09g005390 vs. ExPASy TrEMBL
Match: A0A6J1GQI8 (Dolichyl-phosphate beta-glucosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456187 PE=3 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 1.0e-177
Identity = 311/331 (93.96%), Postives = 319/331 (96.37%), Query Frame = 0

Query: 1   MEYPWTILFILIGLALSFAAGLISTIILEAYRRRFNHEHVEAPAIFEDPNSLKRVPCPSI 60
           MEYPWTILF  I LALS  A LISTIILEAYRRRFNH H+EAPAIFEDPNSLKRVPCPSI
Sbjct: 1   MEYPWTILFFFISLALSIVAALISTIILEAYRRRFNHGHIEAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYLSLIVPAFNEEYRLHGALEETINYLQQRAAKDKTFSYEVIIIDDGSVDGTKGV 120
           FDPAEKY+SLIVPAFNEEYRL GALEETINYLQQRAAKDK+FSYEVIIIDDGSVDGTKGV
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLRGALEETINYLQQRAAKDKSFSYEVIIIDDGSVDGTKGV 120

Query: 121 AFTFVRKYSVENVRVILLGKNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180
           AF FVRKYSVENVRVILLG+NHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ
Sbjct: 121 AFNFVRKYSVENVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180

Query: 181 IHAVPIVKKEASRDPSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240
           IHAV +VKKE SRDPS+RISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA
Sbjct: 181 IHAVAVVKKEGSRDPSIRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240

Query: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMIEVSVNWSEIPG 300
           GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISM+EVSVNWSEIPG
Sbjct: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMVEVSVNWSEIPG 300

Query: 301 SKLSPRSILNMLWELALMSMGYRTGIWRIST 332
           SKLSPRSILNMLWELALMS+GYRTG WRIST
Sbjct: 301 SKLSPRSILNMLWELALMSVGYRTGTWRIST 331

BLAST of Lcy09g005390 vs. ExPASy TrEMBL
Match: A0A6J1JRS3 (Dolichyl-phosphate beta-glucosyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111487753 PE=3 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 1.4e-176
Identity = 311/331 (93.96%), Postives = 320/331 (96.68%), Query Frame = 0

Query: 1   MEYPWTILFILIGLALSFAAGLISTIILEAYRRRFNHEHVEAPAIFEDPNSLKRVPCPSI 60
           MEYP TILFILI LALS  A LIS IILEAYRRRFNH H+EAPAIFEDPNSLKRVPCPSI
Sbjct: 1   MEYPRTILFILISLALSIVAALISAIILEAYRRRFNHGHIEAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYLSLIVPAFNEEYRLHGALEETINYLQQRAAKDKTFSYEVIIIDDGSVDGTKGV 120
           FDPAEKY+SLIVPAFNEEYRL GALEETINYLQQRAAKDK+FSYEVIIIDDGSVDGTKGV
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLRGALEETINYLQQRAAKDKSFSYEVIIIDDGSVDGTKGV 120

Query: 121 AFTFVRKYSVENVRVILLGKNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180
           AF FVRKYSVENVRVILLG+NHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ
Sbjct: 121 AFNFVRKYSVENVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180

Query: 181 IHAVPIVKKEASRDPSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240
           IHAV +VKKE SRDPS+RISDIPITAFGSRAHL+EKALATRKWYRNFLMKGFHLVVLLTA
Sbjct: 181 IHAVAVVKKEGSRDPSIRISDIPITAFGSRAHLKEKALATRKWYRNFLMKGFHLVVLLTA 240

Query: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMIEVSVNWSEIPG 300
           GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISM+EVSVNWSEIPG
Sbjct: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMVEVSVNWSEIPG 300

Query: 301 SKLSPRSILNMLWELALMSMGYRTGIWRIST 332
           SKLSPRSILNMLWELALMS+GYRTGIWRIST
Sbjct: 301 SKLSPRSILNMLWELALMSVGYRTGIWRIST 331

BLAST of Lcy09g005390 vs. ExPASy TrEMBL
Match: A0A6J1C019 (Dolichyl-phosphate beta-glucosyltransferase OS=Momordica charantia OX=3673 GN=LOC111006250 PE=3 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 2.0e-170
Identity = 300/330 (90.91%), Postives = 312/330 (94.55%), Query Frame = 0

Query: 1   MEYPWTILFILIGLALSFAAGLISTIILEAYRRRFNHEHVEAPAIFEDPNSLKRVPCPSI 60
           MEYPWTI FILI L  +FAAGL+S IIL+AYRRRFNHEHVEAPAI EDPNSLKRVPCPSI
Sbjct: 1   MEYPWTIFFILISLFFTFAAGLVSAIILKAYRRRFNHEHVEAPAILEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYLSLIVPAFNEEYRLHGALEETINYLQQRAAKDKTFSYEVIIIDDGSVDGTKGV 120
           FDPAEKY+SLIVPA+NEEYRLHGALEETINYL+QRAAKDKTFSYEV+I+DDGSVD TKGV
Sbjct: 61  FDPAEKYMSLIVPAYNEEYRLHGALEETINYLRQRAAKDKTFSYEVVIVDDGSVDATKGV 120

Query: 121 AFTFVRKYSVENVRVILLGKNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180
           AF  VRKYSVENVRVILLGKNHGKGEA RKGMLHSRGELLLMLDADGAT ITDLEKLENQ
Sbjct: 121 AFNLVRKYSVENVRVILLGKNHGKGEATRKGMLHSRGELLLMLDADGATNITDLEKLENQ 180

Query: 181 IHAVPIVKKEASRDPSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240
           IHA   VKKEASRD SVRISD+PITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA
Sbjct: 181 IHAE--VKKEASRDSSVRISDVPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240

Query: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMIEVSVNWSEIPG 300
           GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGI M+EVSVNWSEIPG
Sbjct: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGIPMLEVSVNWSEIPG 300

Query: 301 SKLSPRSILNMLWELALMSMGYRTGIWRIS 331
           SKL P SILNMLWELALM++GYRTGIWRIS
Sbjct: 301 SKLGPWSILNMLWELALMTVGYRTGIWRIS 328

BLAST of Lcy09g005390 vs. ExPASy TrEMBL
Match: A0A0A0KSA3 (Dolichyl-phosphate beta-glucosyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G517110 PE=3 SV=1)

HSP 1 Score: 600.9 bits (1548), Expect = 3.2e-168
Identity = 296/329 (89.97%), Postives = 312/329 (94.83%), Query Frame = 0

Query: 1   MEYPWTILFILIGLALSFAAGLISTIILEAYRRRFNHEHVEAPAIFEDPNSLKRVPCPSI 60
           MEYPWTILFI I LAL F A L+ST+I+EAY+RRFN EH+ APAIFEDPNSLKRVPCPSI
Sbjct: 1   MEYPWTILFIFISLALCFPAALLSTLIIEAYKRRFNREHLGAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYLSLIVPAFNEEYRLHGALEETINYLQQRAAKDKTFSYEVIIIDDGSVDGTKGV 120
           FDPAEKY+SLIVPAFNEEYRL GALEETINYLQQRAAKDK+FSYEVIIIDDGS DGTKG+
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLQGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI 120

Query: 121 AFTFVRKYSVENVRVILLGKNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180
           AF FVRKYSVENVRVILLG+NHGKGEAIRKGMLHSRGE+LLMLDADGATKITDLEKLENQ
Sbjct: 121 AFNFVRKYSVENVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ 180

Query: 181 IHAVPIVKKEASRDPSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240
           IHAV  VKKE SRD SVR+SDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA
Sbjct: 181 IHAV--VKKETSRDSSVRVSDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240

Query: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMIEVSVNWSEIPG 300
           GPGIRDTQCGFKMFTRSAARKLF NIRLKRWCFDVELVYL KYFGI MIEVSVNWSEIPG
Sbjct: 241 GPGIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELVYLSKYFGIPMIEVSVNWSEIPG 300

Query: 301 SKLSPRSILNMLWELALMSMGYRTGIWRI 330
           SKLSP+SILNMLWELALMS+GY+TG+W+I
Sbjct: 301 SKLSPQSILNMLWELALMSIGYKTGLWKI 327

BLAST of Lcy09g005390 vs. ExPASy TrEMBL
Match: A0A1S4E276 (Dolichyl-phosphate beta-glucosyltransferase OS=Cucumis melo OX=3656 GN=LOC103498152 PE=3 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 3.0e-166
Identity = 294/329 (89.36%), Postives = 308/329 (93.62%), Query Frame = 0

Query: 1   MEYPWTILFILIGLALSFAAGLISTIILEAYRRRFNHEHVEAPAIFEDPNSLKRVPCPSI 60
           MEY WTILFI I LAL FAA +IS +I+EA++RRFN EHVEAPAIFEDPNSLKRVPCPSI
Sbjct: 1   MEYLWTILFIFISLALCFAAAIISALIIEAFKRRFNREHVEAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYLSLIVPAFNEEYRLHGALEETINYLQQRAAKDKTFSYEVIIIDDGSVDGTKGV 120
           FDPAEKY+SLIVPAFNEEYRLHGALEETINYLQQRAAKDK+FSYEVIIIDDGS DGTKG+
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLHGALEETINYLQQRAAKDKSFSYEVIIIDDGSADGTKGI 120

Query: 121 AFTFVRKYSVENVRVILLGKNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180
           AF FVRKYSVE VRVILLG+NHGKGEAIRKGMLHSRGE+LLMLDADGATKITDLEKLENQ
Sbjct: 121 AFNFVRKYSVEKVRVILLGRNHGKGEAIRKGMLHSRGEILLMLDADGATKITDLEKLENQ 180

Query: 181 IHAVPIVKKEASRDPSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240
           IHAV   KKE SRD S++ISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLL A
Sbjct: 181 IHAV--AKKETSRDSSIKISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLIA 240

Query: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMIEVSVNWSEIPG 300
           GPGIRDTQCGFKMFTRSAARKLF NIRLKRWCFDVEL YL KYFGI MIEVSVNWSEIPG
Sbjct: 241 GPGIRDTQCGFKMFTRSAARKLFINIRLKRWCFDVELFYLSKYFGIPMIEVSVNWSEIPG 300

Query: 301 SKLSPRSILNMLWELALMSMGYRTGIWRI 330
           SKLSPRSILNMLWELALMS+GY+ GIWRI
Sbjct: 301 SKLSPRSILNMLWELALMSIGYKIGIWRI 327

BLAST of Lcy09g005390 vs. NCBI nr
Match: XP_022953750.1 (dolichyl-phosphate beta-glucosyltransferase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 632.5 bits (1630), Expect = 2.1e-177
Identity = 311/331 (93.96%), Postives = 319/331 (96.37%), Query Frame = 0

Query: 1   MEYPWTILFILIGLALSFAAGLISTIILEAYRRRFNHEHVEAPAIFEDPNSLKRVPCPSI 60
           MEYPWTILF  I LALS  A LISTIILEAYRRRFNH H+EAPAIFEDPNSLKRVPCPSI
Sbjct: 1   MEYPWTILFFFISLALSIVAALISTIILEAYRRRFNHGHIEAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYLSLIVPAFNEEYRLHGALEETINYLQQRAAKDKTFSYEVIIIDDGSVDGTKGV 120
           FDPAEKY+SLIVPAFNEEYRL GALEETINYLQQRAAKDK+FSYEVIIIDDGSVDGTKGV
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLRGALEETINYLQQRAAKDKSFSYEVIIIDDGSVDGTKGV 120

Query: 121 AFTFVRKYSVENVRVILLGKNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180
           AF FVRKYSVENVRVILLG+NHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ
Sbjct: 121 AFNFVRKYSVENVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180

Query: 181 IHAVPIVKKEASRDPSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240
           IHAV +VKKE SRDPS+RISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA
Sbjct: 181 IHAVAVVKKEGSRDPSIRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240

Query: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMIEVSVNWSEIPG 300
           GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISM+EVSVNWSEIPG
Sbjct: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMVEVSVNWSEIPG 300

Query: 301 SKLSPRSILNMLWELALMSMGYRTGIWRIST 332
           SKLSPRSILNMLWELALMS+GYRTG WRIST
Sbjct: 301 SKLSPRSILNMLWELALMSVGYRTGTWRIST 331

BLAST of Lcy09g005390 vs. NCBI nr
Match: XP_022991045.1 (dolichyl-phosphate beta-glucosyltransferase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 628.6 bits (1620), Expect = 3.0e-176
Identity = 311/331 (93.96%), Postives = 320/331 (96.68%), Query Frame = 0

Query: 1   MEYPWTILFILIGLALSFAAGLISTIILEAYRRRFNHEHVEAPAIFEDPNSLKRVPCPSI 60
           MEYP TILFILI LALS  A LIS IILEAYRRRFNH H+EAPAIFEDPNSLKRVPCPSI
Sbjct: 1   MEYPRTILFILISLALSIVAALISAIILEAYRRRFNHGHIEAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYLSLIVPAFNEEYRLHGALEETINYLQQRAAKDKTFSYEVIIIDDGSVDGTKGV 120
           FDPAEKY+SLIVPAFNEEYRL GALEETINYLQQRAAKDK+FSYEVIIIDDGSVDGTKGV
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLRGALEETINYLQQRAAKDKSFSYEVIIIDDGSVDGTKGV 120

Query: 121 AFTFVRKYSVENVRVILLGKNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180
           AF FVRKYSVENVRVILLG+NHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ
Sbjct: 121 AFNFVRKYSVENVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180

Query: 181 IHAVPIVKKEASRDPSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240
           IHAV +VKKE SRDPS+RISDIPITAFGSRAHL+EKALATRKWYRNFLMKGFHLVVLLTA
Sbjct: 181 IHAVAVVKKEGSRDPSIRISDIPITAFGSRAHLKEKALATRKWYRNFLMKGFHLVVLLTA 240

Query: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMIEVSVNWSEIPG 300
           GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISM+EVSVNWSEIPG
Sbjct: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMVEVSVNWSEIPG 300

Query: 301 SKLSPRSILNMLWELALMSMGYRTGIWRIST 332
           SKLSPRSILNMLWELALMS+GYRTGIWRIST
Sbjct: 301 SKLSPRSILNMLWELALMSVGYRTGIWRIST 331

BLAST of Lcy09g005390 vs. NCBI nr
Match: KAG7033054.1 (Dolichyl-phosphate beta-glucosyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 628.6 bits (1620), Expect = 3.0e-176
Identity = 309/331 (93.35%), Postives = 318/331 (96.07%), Query Frame = 0

Query: 1   MEYPWTILFILIGLALSFAAGLISTIILEAYRRRFNHEHVEAPAIFEDPNSLKRVPCPSI 60
           MEYPWTILF  I LALS  A LIS IILEAYRRRFNH H+EAPAIFEDPNSLKRVPCPSI
Sbjct: 1   MEYPWTILFFFISLALSIVAALISAIILEAYRRRFNHGHIEAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYLSLIVPAFNEEYRLHGALEETINYLQQRAAKDKTFSYEVIIIDDGSVDGTKGV 120
           FDPAEKY+SLIVPAFNEEYRL GALEETINYL+QRAAKDK+FSYEVIIIDDGSVDGTKGV
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLRGALEETINYLRQRAAKDKSFSYEVIIIDDGSVDGTKGV 120

Query: 121 AFTFVRKYSVENVRVILLGKNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180
           AF FVRKYSVENVRVILLG+NHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ
Sbjct: 121 AFNFVRKYSVENVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180

Query: 181 IHAVPIVKKEASRDPSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240
           IHAV +VKKE SRDPS+RISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA
Sbjct: 181 IHAVVVVKKEGSRDPSIRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240

Query: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMIEVSVNWSEIPG 300
           GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISM+EVSVNWSEIPG
Sbjct: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMVEVSVNWSEIPG 300

Query: 301 SKLSPRSILNMLWELALMSMGYRTGIWRIST 332
           SKLSPRSILNMLWELALMS+GYRTG WRIST
Sbjct: 301 SKLSPRSILNMLWELALMSVGYRTGTWRIST 331

BLAST of Lcy09g005390 vs. NCBI nr
Match: KAG6602376.1 (Dolichyl-phosphate beta-glucosyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 625.9 bits (1613), Expect = 1.9e-175
Identity = 308/331 (93.05%), Postives = 317/331 (95.77%), Query Frame = 0

Query: 1   MEYPWTILFILIGLALSFAAGLISTIILEAYRRRFNHEHVEAPAIFEDPNSLKRVPCPSI 60
           MEYPWTILF  I LALS  A LIS IILEAYRRRFNH H+EAPAIFEDPNSLKRVPCPSI
Sbjct: 1   MEYPWTILFFFISLALSIVAALISAIILEAYRRRFNHGHIEAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYLSLIVPAFNEEYRLHGALEETINYLQQRAAKDKTFSYEVIIIDDGSVDGTKGV 120
           FDPAEKY+SLIVPAFNEEYRL GALEETINYL+QRAAKDK+FSYEVIIIDDGSVDGTKGV
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLRGALEETINYLRQRAAKDKSFSYEVIIIDDGSVDGTKGV 120

Query: 121 AFTFVRKYSVENVRVILLGKNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180
           AF FVRKYSVENVRVILLG+NHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ
Sbjct: 121 AFNFVRKYSVENVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180

Query: 181 IHAVPIVKKEASRDPSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240
           I AV +VKKE SRDPS+RISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA
Sbjct: 181 IQAVAVVKKEGSRDPSIRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240

Query: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMIEVSVNWSEIPG 300
           GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISM+EVSVNWSEIPG
Sbjct: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMVEVSVNWSEIPG 300

Query: 301 SKLSPRSILNMLWELALMSMGYRTGIWRIST 332
           SKLSPRSILNMLWELALMS+GYRTG WRIST
Sbjct: 301 SKLSPRSILNMLWELALMSVGYRTGTWRIST 331

BLAST of Lcy09g005390 vs. NCBI nr
Match: XP_038889980.1 (dolichyl-phosphate beta-glucosyltransferase-like [Benincasa hispida])

HSP 1 Score: 619.4 bits (1596), Expect = 1.8e-173
Identity = 306/329 (93.01%), Postives = 317/329 (96.35%), Query Frame = 0

Query: 1   MEYPWTILFILIGLALSFAAGLISTIILEAYRRRFNHEHVEAPAIFEDPNSLKRVPCPSI 60
           MEYPWTILFILI LAL  A GLIS +I+EAYRRRFNH+HVEAPAIFEDPNSLKRVPCPSI
Sbjct: 1   MEYPWTILFILISLALCIAVGLISAVIIEAYRRRFNHQHVEAPAIFEDPNSLKRVPCPSI 60

Query: 61  FDPAEKYLSLIVPAFNEEYRLHGALEETINYLQQRAAKDKTFSYEVIIIDDGSVDGTKGV 120
           FDPAEKY+SLIVPAFNEEYRLHGALEETINYLQQRAAKDK++SYEVIIIDDGSVDGTKGV
Sbjct: 61  FDPAEKYMSLIVPAFNEEYRLHGALEETINYLQQRAAKDKSYSYEVIIIDDGSVDGTKGV 120

Query: 121 AFTFVRKYSVENVRVILLGKNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180
           AF FVRKYSVENVRVILLG+NHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ
Sbjct: 121 AFNFVRKYSVENVRVILLGRNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180

Query: 181 IHAVPIVKKEASRDPSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240
           IHAV  VKKEASRDPS+RISDIPI AFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA
Sbjct: 181 IHAV--VKKEASRDPSIRISDIPIAAFGSRAHLEEKALATRKWYRNFLMKGFHLVVLLTA 240

Query: 241 GPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMIEVSVNWSEIPG 300
           GPGIRDTQCGFKMFTRSA RKLF+NIRLKRWCFDVELVYLCK+FGI MIEVSVNWSEIPG
Sbjct: 241 GPGIRDTQCGFKMFTRSAGRKLFSNIRLKRWCFDVELVYLCKFFGIPMIEVSVNWSEIPG 300

Query: 301 SKLSPRSILNMLWELALMSMGYRTGIWRI 330
           SKLSPRSILNMLWELALMS+GYRT IWRI
Sbjct: 301 SKLSPRSILNMLWELALMSVGYRTRIWRI 327

BLAST of Lcy09g005390 vs. TAIR 10
Match: AT2G39630.1 (Nucleotide-diphospho-sugar transferases superfamily protein )

HSP 1 Score: 475.7 bits (1223), Expect = 3.0e-134
Identity = 231/333 (69.37%), Postives = 276/333 (82.88%), Query Frame = 0

Query: 1   MEYPWTILFILIGLALSFAAGLISTIILEAYRRRFNHEHVEAPAIFEDPNSLKRVPCPSI 60
           ME+  T+    + L L    G +S ++ EA+RRR ++  VE     +DP S+K +PCP I
Sbjct: 1   MEFLVTVAEFSLWLLLIVLFGFLSVVVFEAWRRRHSNVSVETVTTLDDPKSIKPIPCPHI 60

Query: 61  FDPAEKYLSLIVPAFNEEYRLHGALEETINYLQQRAAKDKTFSYEVIIIDDGSVDGTKGV 120
            DPAEKYLSLIVPA+NEE RL  ALEET++YLQ RA++DK+FS+EV+I+DDGSVDGTK V
Sbjct: 61  TDPAEKYLSLIVPAYNEELRLPAALEETMDYLQDRASRDKSFSFEVVIVDDGSVDGTKRV 120

Query: 121 AFTFVRKYSVENVRVILLGKNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180
           AF F+RKY+++N+RVI LGKN GKGEAIRKGMLHSRG+LLLMLDADGATK+TDLEKLENQ
Sbjct: 121 AFDFIRKYTIDNIRVIPLGKNQGKGEAIRKGMLHSRGQLLLMLDADGATKVTDLEKLENQ 180

Query: 181 IHAVP----IVKKEASRDPSVRISDIPITAFGSRAHLEEKALATRKWYRNFLMKGFHLVV 240
           I+AV      ++  AS+D   +I D+ ++AFGSRAHLEEKALATRKWYRNFLMKGFHLVV
Sbjct: 181 INAVAREEYSIRNPASKDMDFKIGDVQVSAFGSRAHLEEKALATRKWYRNFLMKGFHLVV 240

Query: 241 LLTAGPGIRDTQCGFKMFTRSAARKLFTNIRLKRWCFDVELVYLCKYFGISMIEVSVNWS 300
           LL AGPGIRDTQCGFKMFTR+AAR+LFTN+ LKRWCFDVELVYLCK F I M+E+SV WS
Sbjct: 241 LLAAGPGIRDTQCGFKMFTRAAARRLFTNVHLKRWCFDVELVYLCKRFNIPMVEISVKWS 300

Query: 301 EIPGSKLSPRSILNMLWELALMSMGYRTGIWRI 330
           EIPGSK+S  SI NMLWELALMS+GYRTG+W+I
Sbjct: 301 EIPGSKVSMLSIPNMLWELALMSVGYRTGMWKI 333

BLAST of Lcy09g005390 vs. TAIR 10
Match: AT2G39630.2 (Nucleotide-diphospho-sugar transferases superfamily protein )

HSP 1 Score: 280.0 bits (715), Expect = 2.4e-75
Identity = 140/224 (62.50%), Postives = 176/224 (78.57%), Query Frame = 0

Query: 1   MEYPWTILFILIGLALSFAAGLISTIILEAYRRRFNHEHVEAPAIFEDPNSLKRVPCPSI 60
           ME+  T+    + L L    G +S ++ EA+RRR ++  VE     +DP S+K +PCP I
Sbjct: 1   MEFLVTVAEFSLWLLLIVLFGFLSVVVFEAWRRRHSNVSVETVTTLDDPKSIKPIPCPHI 60

Query: 61  FDPAEKYLSLIVPAFNEEYRLHGALEETINYLQQRAAKDKTFSYEVIIIDDGSVDGTKGV 120
            DPAEKYLSLIVPA+NEE RL  ALEET++YLQ RA++DK+FS+EV+I+DDGSVDGTK V
Sbjct: 61  TDPAEKYLSLIVPAYNEELRLPAALEETMDYLQDRASRDKSFSFEVVIVDDGSVDGTKRV 120

Query: 121 AFTFVRKYSVENVRVILLGKNHGKGEAIRKGMLHSRGELLLMLDADGATKITDLEKLENQ 180
           AF F+RKY+++N+RVI LGKN GKGEAIRKGMLHSRG+LLLMLDADGATK+TDLEKLENQ
Sbjct: 121 AFDFIRKYTIDNIRVIPLGKNQGKGEAIRKGMLHSRGQLLLMLDADGATKVTDLEKLENQ 180

Query: 181 IHAVP----IVKKEASRDPSVRISDIPITAFGSRAHLEEKALAT 221
           I+AV      ++  AS+D   +I D+ ++AFGSRAHLEEKALAT
Sbjct: 181 INAVAREEYSIRNPASKDMDFKIGDVQVSAFGSRAHLEEKALAT 224

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9Y6731.8e-6743.47Dolichyl-phosphate beta-glucosyltransferase OS=Homo sapiens OX=9606 GN=ALG5 PE=1... [more]
Q9VLQ12.6e-6645.18Dolichyl-phosphate beta-glucosyltransferase OS=Drosophila melanogaster OX=7227 G... [more]
Q9DB257.6e-6642.86Dolichyl-phosphate beta-glucosyltransferase OS=Mus musculus OX=10090 GN=Alg5 PE=... [more]
A2DZE87.4e-6143.42Dolichyl-phosphate beta-glucosyltransferase ALG5A OS=Trichomonas vaginalis OX=57... [more]
A2ELE64.8e-6040.57Dolichyl-phosphate beta-glucosyltransferase ALG5C OS=Trichomonas vaginalis OX=57... [more]
Match NameE-valueIdentityDescription
A0A6J1GQI81.0e-17793.96Dolichyl-phosphate beta-glucosyltransferase OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1JRS31.4e-17693.96Dolichyl-phosphate beta-glucosyltransferase OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1C0192.0e-17090.91Dolichyl-phosphate beta-glucosyltransferase OS=Momordica charantia OX=3673 GN=LO... [more]
A0A0A0KSA33.2e-16889.97Dolichyl-phosphate beta-glucosyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G... [more]
A0A1S4E2763.0e-16689.36Dolichyl-phosphate beta-glucosyltransferase OS=Cucumis melo OX=3656 GN=LOC103498... [more]
Match NameE-valueIdentityDescription
XP_022953750.12.1e-17793.96dolichyl-phosphate beta-glucosyltransferase-like isoform X1 [Cucurbita moschata][more]
XP_022991045.13.0e-17693.96dolichyl-phosphate beta-glucosyltransferase-like isoform X1 [Cucurbita maxima][more]
KAG7033054.13.0e-17693.35Dolichyl-phosphate beta-glucosyltransferase, partial [Cucurbita argyrosperma sub... [more]
KAG6602376.11.9e-17593.05Dolichyl-phosphate beta-glucosyltransferase, partial [Cucurbita argyrosperma sub... [more]
XP_038889980.11.8e-17393.01dolichyl-phosphate beta-glucosyltransferase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G39630.13.0e-13469.37Nucleotide-diphospho-sugar transferases superfamily protein [more]
AT2G39630.22.4e-7562.50Nucleotide-diphospho-sugar transferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029044Nucleotide-diphospho-sugar transferasesGENE3D3.90.550.10Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain Acoord: 51..322
e-value: 2.9E-32
score: 114.2
IPR029044Nucleotide-diphospho-sugar transferasesSUPERFAMILY53448Nucleotide-diphospho-sugar transferasescoord: 62..314
IPR001173Glycosyltransferase 2-likePFAMPF00535Glycos_transf_2coord: 69..183
e-value: 6.1E-21
score: 74.9
NoneNo IPR availablePANTHERPTHR10859:SF108BNAA03G18660D PROTEINcoord: 20..329
NoneNo IPR availablePANTHERPTHR10859GLYCOSYL TRANSFERASEcoord: 20..329
IPR035518Dolichyl-phosphate beta-glucosyltransferaseCDDcd04188DPG_synthasecoord: 70..302
e-value: 4.84171E-101
score: 294.09

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g005390.1Lcy09g005390.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006487 protein N-linked glycosylation
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004581 dolichyl-phosphate beta-glucosyltransferase activity