Lcy09g005170 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g005170
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionPectate lyase
LocationChr09: 4562045 .. 4564944 (+)
RNA-Seq ExpressionLcy09g005170
SyntenyLcy09g005170
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCTTCTTCTTCTTCTTTTTCAAGCTTTTTCTTTGTTTTGATCTCCAATGGCGGCTTCTCTTTGCTGCTTTTTGCTTCTTTTTTCTGTCTCTTTCTTGTTGACTGCTCCAACTTCTGCTTCCTCGTTGGCTGATGTTCAAGATCCTCATCTTGTCGTTCAAGAAGTTCAAAGGTATATACATGTTTTTTTTTTGTTTCTCGTTTGTTGTTTATGGTGTTCTTAGTTTTTGGGAAAGATATGGATTATGGTGTATCTGTATCGTTTTTAGGGAATGTACTATCACTGTGCAAATATTAAAACGGAATGTGGTTTTTTGTGAGATGTGTTTTAGTTTTTCAAAAATGTAACGATAATGATTATCAAATCCATTCTTATTTATGTTTTTTTTTTTAAAAAAAATTGTAACTTGTAATTACGTTTTTCTCTCTCTTTCTTTGTGATGATTTTTTTTTTTAGATCTTGAAATGAAAAGGTTTAAACACCTACATTATGATTTTTTTTTTTTTTTTTTGAGAATAACCTACATTATGATTATGAAGATGTATAGTGTTGGAATAATATGGTTGACATTTTCTATATACTGATTAATGTATGAGAGAGAGTTTTAAAACCGTGTTTTTATCGGAGGAACGACCACATGTATATAAATAGTTTTTAATACATTCTTTCTTTTCCTTTGCCCCTACTTTCCTTTGACTTTTATCTTTCTCTCTCGAACAATATTTGGTTACATAATGGTGTCTAGGGATCTTTCTACCATACACTATGAAAATTTTGACAACTAAGAGAGAAAAAGGTACAAAATGTGATTTTTCTTATCTCTCCGAATTGTTAACATGAACATAAACTTGGCTGCATACCGGTAGGGATCTCCTTACCTACCTCATTAAAATCTTGACAACTAAGAAAGAGGAAGGGAGGAAATGAGAAATTTTTTTATCTCTCCTAGTTGTCAACATTTTAATAGTGGTATGTAGGGAGATCCCTACATATATACCGGTATGCAGCCAAGTTTTTTCCTGTTAACATTTCAAAGGTGGTACTTATACACCAATATGCAGCAAGTATTGCACTTCTCTCCCCGTCCAAAGTGAGGATATTTGAGCCTACATTTTGAAAAATTATTAAAAAAAAAAAAAAGCAAACAATATAATTGACAGTTTTTGTACCCTTTGAGTATATATATAAGAGATAAATTAAAAGTTGTATTTTAACTAATTAAGAAAAAAATCCTGTATCAAAATCTATTTAACCCTAATAACCATTTGATCAAAGTTAAGAGAAAACATAACTTAGAGGTAATTATTGACATTTATTCTCGACTCTGTTCCAACTTTTCATAATTTCCTTGGTCTGTCTCTAAAAGAAGCTTGCCGTTGTCGTATTTACATTGAAACACAGGAGCATAAATTCATCAAGAAGAAACTTAGGATATTTCTCCTGCGGAACCGGAAACCCAATAGACGACTGCTGGAGATGCGACCCCAATTGGGAAACCAACCGCAAGCGCCTGGCCGACTGCGCCATCGGCTTCGGCAAGAACGCCATCGGCGGCAGAAACGGCCGCTTCTACGTGGTCACCGACTCCGGCGACGACGACCCTCTCAACCCCCGCCCGGGAACCCTCCGCCACGCCGTCATCCAAAACGAGGCCCTGTGGATCATCTTCAAACGCGACATGGTCATCCAACTCAGGCAGGAGCTGGTGATGAACTCGTTCAAGACCATCGACGGCCGTGGGGCCAGCGTCCACGTGGCCGGTGGGCCATGCATAACGATCCACTACGCGACGAACATCATCCTACATGGCATCCACATACACGACTGCAAGAGAGGGGGGAATGGTAACATACGGGACTCCCCACAGCACTCCGGGTGGTGGACCGTATCGGACGGCGACGGAGTCTCGATCTTCGGCGGGCGGCATATCTGGGTTGACCACTGTTCTTTGTCGAATTGCAATGATGGGTTGATCGACGCGATTCATGGATCCACTGCTATTACTATTTCGAATAATTATATGACTCATCATGATAAGGTGATGCTGTTGGGACACAGTGACTCTTATACTCAAGACAAGGATATGCAGGTTACCATTGCTTTCAACCATTTTGGTGAGGGCCTCGTCCAACGAATGCCAAGGTAAATAAAAATCCATGGGTGTTTTTGTTTTGATCTAAAGAACATATCTTTCATCATGGTTTGGCAATGATTTTGCAGATGCAGACACGGGTATTTCCATGTGGTGAACAACGATTACACCCACTGGGAGATGTACGCAATCGGAGGGAGTGCTTCGCCGACAATATACAGCCAAGGCAACAGATTTTTGGCGCCGGACAACCGCTTCCGAAAGCAGGTGACAAAGCACGAGGACGCGCCGGAGAGCGAGTGGCGCCACTGGAACTGGCGGTCGGAGGGCGACATGATGTTGAACGGCGCCTATTTCCTGCAGTCGGGCGCCGGCGCCGCCTCCAGCTACGCCAGAGCTTACAGCCTCAGCGCTAGACCGTCCTCTCTCGTCGGCTCTATCACCGTCACCGCCGGAGCCCTCAACTGCCGCAGAGGCTCCCGCTGCTAGCGCCCCAGCGCTCTAGTTAGTTAAAAAGTTATATAATTAACTGCAAGTAGATGAAACGAAGGAGCAGTTTAATTATTATTAGTTTATGAAGCTAAGATCTGAAATTTTCCTGTTAATTGAATTTCCTTCCAAGTTTTTGGGTTGCCAAAAAAGAAACTGTAATATTTTGGTTAAACTCGGATGTTTGTGAGTGTGAAAGCTAATGGGAAAGTATGAGGAATTACGTTTTTGAGTCTGCAACCACGCTGAGAAAAAAAGGAATGAGTAATATATGTATTCTTGTTCTTTTACCCGTCAATCATGCTGGATCTTTGGAGA

mRNA sequence

TTCTTCTTCTTCTTCTTTTTCAAGCTTTTTCTTTGTTTTGATCTCCAATGGCGGCTTCTCTTTGCTGCTTTTTGCTTCTTTTTTCTGTCTCTTTCTTGTTGACTGCTCCAACTTCTGCTTCCTCGTTGGCTGATGTTCAAGATCCTCATCTTGTCGTTCAAGAAGTTCAAAGGAGCATAAATTCATCAAGAAGAAACTTAGGATATTTCTCCTGCGGAACCGGAAACCCAATAGACGACTGCTGGAGATGCGACCCCAATTGGGAAACCAACCGCAAGCGCCTGGCCGACTGCGCCATCGGCTTCGGCAAGAACGCCATCGGCGGCAGAAACGGCCGCTTCTACGTGGTCACCGACTCCGGCGACGACGACCCTCTCAACCCCCGCCCGGGAACCCTCCGCCACGCCGTCATCCAAAACGAGGCCCTGTGGATCATCTTCAAACGCGACATGGTCATCCAACTCAGGCAGGAGCTGGTGATGAACTCGTTCAAGACCATCGACGGCCGTGGGGCCAGCGTCCACGTGGCCGGTGGGCCATGCATAACGATCCACTACGCGACGAACATCATCCTACATGGCATCCACATACACGACTGCAAGAGAGGGGGGAATGGTAACATACGGGACTCCCCACAGCACTCCGGGTGGTGGACCGTATCGGACGGCGACGGAGTCTCGATCTTCGGCGGGCGGCATATCTGGGTTGACCACTGTTCTTTGTCGAATTGCAATGATGGGTTGATCGACGCGATTCATGGATCCACTGCTATTACTATTTCGAATAATTATATGACTCATCATGATAAGGTGATGCTGTTGGGACACAGTGACTCTTATACTCAAGACAAGGATATGCAGGTTACCATTGCTTTCAACCATTTTGGTGAGGGCCTCGTCCAACGAATGCCAAGATGCAGACACGGGTATTTCCATGTGGTGAACAACGATTACACCCACTGGGAGATGTACGCAATCGGAGGGAGTGCTTCGCCGACAATATACAGCCAAGGCAACAGATTTTTGGCGCCGGACAACCGCTTCCGAAAGCAGGTGACAAAGCACGAGGACGCGCCGGAGAGCGAGTGGCGCCACTGGAACTGGCGGTCGGAGGGCGACATGATGTTGAACGGCGCCTATTTCCTGCAGTCGGGCGCCGGCGCCGCCTCCAGCTACGCCAGAGCTTACAGCCTCAGCGCTAGACCGTCCTCTCTCGTCGGCTCTATCACCGTCACCGCCGGAGCCCTCAACTGCCGCAGAGGCTCCCGCTGCTAGCGCCCCAGCGCTCTAGTTAGTTAAAAAGTTATATAATTAACTGCAAGTAGATGAAACGAAGGAGCAGTTTAATTATTATTAGTTTATGAAGCTAAGATCTGAAATTTTCCTGTTAATTGAATTTCCTTCCAAGTTTTTGGGTTGCCAAAAAAGAAACTGTAATATTTTGGTTAAACTCGGATGTTTGTGAGTGTGAAAGCTAATGGGAAAGTATGAGGAATTACGTTTTTGAGTCTGCAACCACGCTGAGAAAAAAAGGAATGAGTAATATATGTATTCTTGTTCTTTTACCCGTCAATCATGCTGGATCTTTGGAGA

Coding sequence (CDS)

ATGGCGGCTTCTCTTTGCTGCTTTTTGCTTCTTTTTTCTGTCTCTTTCTTGTTGACTGCTCCAACTTCTGCTTCCTCGTTGGCTGATGTTCAAGATCCTCATCTTGTCGTTCAAGAAGTTCAAAGGAGCATAAATTCATCAAGAAGAAACTTAGGATATTTCTCCTGCGGAACCGGAAACCCAATAGACGACTGCTGGAGATGCGACCCCAATTGGGAAACCAACCGCAAGCGCCTGGCCGACTGCGCCATCGGCTTCGGCAAGAACGCCATCGGCGGCAGAAACGGCCGCTTCTACGTGGTCACCGACTCCGGCGACGACGACCCTCTCAACCCCCGCCCGGGAACCCTCCGCCACGCCGTCATCCAAAACGAGGCCCTGTGGATCATCTTCAAACGCGACATGGTCATCCAACTCAGGCAGGAGCTGGTGATGAACTCGTTCAAGACCATCGACGGCCGTGGGGCCAGCGTCCACGTGGCCGGTGGGCCATGCATAACGATCCACTACGCGACGAACATCATCCTACATGGCATCCACATACACGACTGCAAGAGAGGGGGGAATGGTAACATACGGGACTCCCCACAGCACTCCGGGTGGTGGACCGTATCGGACGGCGACGGAGTCTCGATCTTCGGCGGGCGGCATATCTGGGTTGACCACTGTTCTTTGTCGAATTGCAATGATGGGTTGATCGACGCGATTCATGGATCCACTGCTATTACTATTTCGAATAATTATATGACTCATCATGATAAGGTGATGCTGTTGGGACACAGTGACTCTTATACTCAAGACAAGGATATGCAGGTTACCATTGCTTTCAACCATTTTGGTGAGGGCCTCGTCCAACGAATGCCAAGATGCAGACACGGGTATTTCCATGTGGTGAACAACGATTACACCCACTGGGAGATGTACGCAATCGGAGGGAGTGCTTCGCCGACAATATACAGCCAAGGCAACAGATTTTTGGCGCCGGACAACCGCTTCCGAAAGCAGGTGACAAAGCACGAGGACGCGCCGGAGAGCGAGTGGCGCCACTGGAACTGGCGGTCGGAGGGCGACATGATGTTGAACGGCGCCTATTTCCTGCAGTCGGGCGCCGGCGCCGCCTCCAGCTACGCCAGAGCTTACAGCCTCAGCGCTAGACCGTCCTCTCTCGTCGGCTCTATCACCGTCACCGCCGGAGCCCTCAACTGCCGCAGAGGCTCCCGCTGCTAG

Protein sequence

MAASLCCFLLLFSVSFLLTAPTSASSLADVQDPHLVVQEVQRSINSSRRNLGYFSCGTGNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDSGDDDPLNPRPGTLRHAVIQNEALWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIILHGIHIHDCKRGGNGNIRDSPQHSGWWTVSDGDGVSIFGGRHIWVDHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASPTIYSQGNRFLAPDNRFRKQVTKHEDAPESEWRHWNWRSEGDMMLNGAYFLQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC
Homology
BLAST of Lcy09g005170 vs. ExPASy Swiss-Prot
Match: O24554 (Pectate lyase OS=Zinnia violacea OX=34245 PE=1 SV=1)

HSP 1 Score: 684.1 bits (1764), Expect = 9.7e-196
Identity = 319/399 (79.95%), Postives = 355/399 (88.97%), Query Frame = 0

Query: 10  LLFSVSFLLTAPTSASSLADVQDPHLVVQEVQRSINSSRRNLGYFSCGTGNPIDDCWRCD 69
           L+  +S L  A +S S     + PH +V EV +SIN+SRRNLGY SCGTGNPIDDCWRCD
Sbjct: 8   LILFISSLAIASSSPS-----RTPHAIVNEVHKSINASRRNLGYLSCGTGNPIDDCWRCD 67

Query: 70  PNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDSGDDDPLNPRPGTLRHAVIQNEALWI 129
           PNW  NR+RLADCAIGFGKNA+GGRNGR YVVTD G+DDP+NP PGTLR+AVIQ+E LWI
Sbjct: 68  PNWANNRQRLADCAIGFGKNAMGGRNGRIYVVTDPGNDDPVNPVPGTLRYAVIQDEPLWI 127

Query: 130 IFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIILHGIHIHDCKRGGN 189
           IFKRDMVIQLRQELVMNS KTIDGRG +VH+  GPCITIHYA+NII+HGIHIHDCK+ GN
Sbjct: 128 IFKRDMVIQLRQELVMNSHKTIDGRGVNVHIGNGPCITIHYASNIIIHGIHIHDCKQAGN 187

Query: 190 GNIRDSPQHSGWWTVSDGDGVSIFGGRHIWVDHCSLSNCNDGLIDAIHGSTAITISNNYM 249
           GNIR+SP HSGWWT SDGDG+SIF  + IW+DH SLSNC+DGLIDAIHGSTAITISNNYM
Sbjct: 188 GNIRNSPHHSGWWTQSDGDGISIFASKDIWIDHNSLSNCHDGLIDAIHGSTAITISNNYM 247

Query: 250 THHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYA 309
           THHDKVMLLGHSDSYTQDK+MQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYA
Sbjct: 248 THHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYA 307

Query: 310 IGGSASPTIYSQGNRFLAPDNRFRKQVTKHEDAPESEWRHWNWRSEGDMMLNGAYFLQSG 369
           IGGSASPTIYSQGNRFLAP+ RF K+VTKHE+APESEW++WNWRSEGD+MLNGAYF +SG
Sbjct: 308 IGGSASPTIYSQGNRFLAPNTRFDKEVTKHENAPESEWKNWNWRSEGDLMLNGAYFRESG 367

Query: 370 AGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 409
             AASS+ARA SLS RPS+LV S+T +AGAL CR+GSRC
Sbjct: 368 GRAASSFARASSLSGRPSTLVASMTRSAGALVCRKGSRC 401

BLAST of Lcy09g005170 vs. ExPASy Swiss-Prot
Match: Q9LTZ0 (Putative pectate lyase 11 OS=Arabidopsis thaliana OX=3702 GN=At3g27400 PE=3 SV=2)

HSP 1 Score: 677.6 bits (1747), Expect = 9.0e-194
Identity = 318/403 (78.91%), Postives = 356/403 (88.34%), Query Frame = 0

Query: 8   FLLLFSVSFLLTAPTSASSLAD-VQDPHLVVQEVQRSI-NSSRRNLGYFSCGTGNPIDDC 67
           FLLL ++   L     +SSL D VQDP+LVV +V RS+ N+SRR+L Y SC TGNPIDDC
Sbjct: 13  FLLLLTIGNTL---AFSSSLPDHVQDPNLVVDDVNRSVFNASRRSLAYLSCRTGNPIDDC 72

Query: 68  WRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDSGDDDPLNPRPGTLRHAVIQNE 127
           WRCDPNWETNR+RLADCAIGFGKNAIGGR GR YVVTD  +DDP+NPRPGTLR+AV Q E
Sbjct: 73  WRCDPNWETNRQRLADCAIGFGKNAIGGRKGRIYVVTDPANDDPVNPRPGTLRYAVTQEE 132

Query: 128 ALWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIILHGIHIHDCK 187
            LWIIFKRDMVI+L++EL++ SFKTIDGRG+SVH+  GPC+ IHYATNII+HGI+IHDCK
Sbjct: 133 PLWIIFKRDMVIRLKKELIITSFKTIDGRGSSVHITDGPCLKIHYATNIIIHGINIHDCK 192

Query: 188 RGGNGNIRDSPQHSGWWTVSDGDGVSIFGGRHIWVDHCSLSNCNDGLIDAIHGSTAITIS 247
            G  G I+D P H+GWW  SDGD V+IFGG+H+W+DHCSLSNC+DGLIDAIHGSTAITIS
Sbjct: 193 PGSGGMIKDGPHHTGWWMQSDGDAVAIFGGKHVWIDHCSLSNCDDGLIDAIHGSTAITIS 252

Query: 248 NNYMTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 307
           NN+MTHHDKVMLLGHSDSYTQDK+MQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW
Sbjct: 253 NNHMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 312

Query: 308 EMYAIGGSASPTIYSQGNRFLAPDNRFRKQVTKHEDAPESEWRHWNWRSEGDMMLNGAYF 367
           EMYAIGGSASPTIYSQGNRFLAP+ RF K+VTKHEDAPES+WR WNWRSEGDM+LNGAYF
Sbjct: 313 EMYAIGGSASPTIYSQGNRFLAPNTRFNKEVTKHEDAPESKWRDWNWRSEGDMLLNGAYF 372

Query: 368 LQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 409
            +SGA A S+YARA SLSARPSSLVGSIT TAG L+CRRG RC
Sbjct: 373 RESGAEAPSTYARASSLSARPSSLVGSITTTAGTLSCRRGRRC 412

BLAST of Lcy09g005170 vs. ExPASy Swiss-Prot
Match: Q9C5M8 (Probable pectate lyase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g24780 PE=2 SV=2)

HSP 1 Score: 664.8 bits (1714), Expect = 6.1e-190
Identity = 309/403 (76.67%), Postives = 355/403 (88.09%), Query Frame = 0

Query: 9   LLLFSVSFLLTAPTSASSLADVQDPHLVVQEVQRSINSS---RRNLGYFSCGTGNPIDDC 68
           L +  VSFLL AP   SS   V DP  VV+EV +SIN+S   RR LGY SC TGNPIDDC
Sbjct: 8   LFITIVSFLLYAPLFLSS--PVPDPESVVEEVHKSINASVAGRRKLGYLSCTTGNPIDDC 67

Query: 69  WRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDSGDDDPLNPRPGTLRHAVIQNE 128
           WRCDP+WE +R+RLADCAIGFGKNAIGGR+GR YVVTDSG+D+P++P+PGTLRHAV+Q+E
Sbjct: 68  WRCDPHWEQHRQRLADCAIGFGKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVVQDE 127

Query: 129 ALWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIILHGIHIHDCK 188
            LWIIF+RDM IQL++EL+MNSFKTIDGRGASVH++GGPCITI Y TNII+HGIHIHDCK
Sbjct: 128 PLWIIFQRDMTIQLKEELIMNSFKTIDGRGASVHISGGPCITIQYVTNIIIHGIHIHDCK 187

Query: 189 RGGNGNIRDSPQHSGWWTVSDGDGVSIFGGRHIWVDHCSLSNCNDGLIDAIHGSTAITIS 248
           +GGN  +R SP+H GW T+SDGDGVSIFGG H+WVDHCS SNC DGLIDAI GSTAIT+S
Sbjct: 188 QGGNAMVRSSPRHFGWRTISDGDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAITLS 247

Query: 249 NNYMTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 308
           NN+MTHHDKVMLLGHSD+Y++DK+MQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW
Sbjct: 248 NNHMTHHDKVMLLGHSDTYSRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 307

Query: 309 EMYAIGGSASPTIYSQGNRFLAPDNRFRKQVTKHEDAPESEWRHWNWRSEGDMMLNGAYF 368
           EMYAIGGSA+PTI SQGNRFLAP+ RF K+VTKHEDAPESEW+ WNWRS GD++LNGA+F
Sbjct: 308 EMYAIGGSANPTINSQGNRFLAPNIRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNGAFF 367

Query: 369 LQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 409
             SG  A+SSYA+A SL A+PSSLVG +T T+GALNCR+GSRC
Sbjct: 368 TPSGGAASSSYAKASSLGAKPSSLVGPLTSTSGALNCRKGSRC 408

BLAST of Lcy09g005170 vs. ExPASy Swiss-Prot
Match: P24396 (Probable pectate lyase P18 OS=Solanum lycopersicum OX=4081 GN=9612 PE=2 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 2.3e-189
Identity = 314/403 (77.92%), Postives = 354/403 (87.84%), Query Frame = 0

Query: 10  LLFSVSFLLTAPTSASSLADVQDPHLVVQEVQRSINSS--RRNLGYFSCGTGNPIDDCWR 69
           L F+ S LL AP     ++ +QDP LVVQ+V RSIN+S  RRNLGY SCG+GNPID    
Sbjct: 4   LFFTFSLLLLAPLLV--ISSIQDPELVVQDVHRSINASLTRRNLGYLSCGSGNPIDRLLA 63

Query: 70  CDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDSGDDDPLNPRPGTLRHAVIQNEAL 129
             P         + CAIGFGKNAIGG+NGR YVVTDSG+DDP+NP+PGTLRHAVIQ+E L
Sbjct: 64  MQPQLGKKSPAFSYCAIGFGKNAIGGKNGRIYVVTDSGNDDPVNPKPGTLRHAVIQDEPL 123

Query: 130 WIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIILHGIHIHDCKRG 189
           WIIFKRDMVIQL+QELVMNS+KTIDGRGASVH++GGPCITIH+ +NII+HGI+IHDCK+ 
Sbjct: 124 WIIFKRDMVIQLKQELVMNSYKTIDGRGASVHISGGPCITIHHTSNIIIHGINIHDCKQS 183

Query: 190 GNGNIRDSPQHSGWWTVSDGDGVSIFGGRHIWVDHCSLSNCNDGLIDAIHGSTAITISNN 249
           GNGNIRDSP HSGWW VSDGDG+SIFGG++IWVDHCSLSNC+DGLIDAIHGSTAITISNN
Sbjct: 184 GNGNIRDSPNHSGWWDVSDGDGISIFGGKNIWVDHCSLSNCHDGLIDAIHGSTAITISNN 243

Query: 250 YMTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM 309
           Y THHDKVMLLGHSDS+TQDK MQVT+AFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM
Sbjct: 244 YFTHHDKVMLLGHSDSFTQDKGMQVTVAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEM 303

Query: 310 YAIGGSASPTIYSQGNRFLAPDNRFRKQVTKHEDAPESEWRHWNWRSEGDMMLNGAYFLQ 369
           YAIGGSA+PTI SQGNRFLAP+ ++RK+VTKHEDAPES+WR WNWRSEGD+MLNGAYF Q
Sbjct: 304 YAIGGSAAPTINSQGNRFLAPNEKYRKEVTKHEDAPESQWRSWNWRSEGDLMLNGAYFRQ 363

Query: 370 SGAGAASS--YARAYSLSARPSSLVGSITVTAGALNCRRGSRC 409
           +GAGA+SS  YARA SLSARPSSLVGSIT  AG +NC++GSRC
Sbjct: 364 TGAGASSSSTYARASSLSARPSSLVGSITTNAGPVNCKKGSRC 404

BLAST of Lcy09g005170 vs. ExPASy Swiss-Prot
Match: Q93Z25 (Probable pectate lyase 22 OS=Arabidopsis thaliana OX=3702 GN=At5g63180 PE=2 SV=1)

HSP 1 Score: 658.3 bits (1697), Expect = 5.7e-188
Identity = 313/410 (76.34%), Postives = 356/410 (86.83%), Query Frame = 0

Query: 3   ASLCCFLLLFSVSFLLTAPTSASSLADVQDPHLVVQEVQRSINS--SRRNLGYFSCGTGN 62
           +S   F L+F    L +A   ASSL  V DP LVV+EV R IN   SRR LG+FSCG+GN
Sbjct: 26  SSYLSFALIFFCCILFSA--LASSL-PVSDPELVVEEVHRKINESISRRKLGFFSCGSGN 85

Query: 63  PIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDSGDDDPLNPRPGTLRHA 122
           PIDDCWRCD +WE NRKRLADC IGFGKNAIGGR+G  YVVTD G+DDP+NPRPGTLR+A
Sbjct: 86  PIDDCWRCDKDWEKNRKRLADCGIGFGKNAIGGRDGEIYVVTDPGNDDPVNPRPGTLRYA 145

Query: 123 VIQNEALWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIILHGIH 182
           VIQ+E LWIIFKRDM IQL++EL+MNSFKT+DGRGASVH++GGPCITI Y TNII+HG+H
Sbjct: 146 VIQDEPLWIIFKRDMTIQLKEELIMNSFKTLDGRGASVHISGGPCITIQYVTNIIIHGLH 205

Query: 183 IHDCKRGGNGNIRDSPQHSGWWTVSDGDGVSIFGGRHIWVDHCSLSNCNDGLIDAIHGST 242
           IHDCK+GGN  +RDSP+H G+ TVSDGDGVSIFGG H+WVDHCSLSNCNDGLIDAI GST
Sbjct: 206 IHDCKQGGNTYVRDSPEHYGYRTVSDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIRGST 265

Query: 243 AITISNNYMTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN 302
           AITISNNY+THH+KVMLLGHSD+Y QDK+MQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN
Sbjct: 266 AITISNNYLTHHNKVMLLGHSDTYEQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN 325

Query: 303 DYTHWEMYAIGGSASPTIYSQGNRFLAPDNRFRKQVTKHEDAPESEWRHWNWRSEGDMML 362
           DYTHWEMYAIGGSA+PTI SQGNRFLAPD+   K+VTKHEDAPE EWR+WNWRSEGD++L
Sbjct: 326 DYTHWEMYAIGGSANPTINSQGNRFLAPDDSSSKEVTKHEDAPEDEWRNWNWRSEGDLLL 385

Query: 363 NGAYFLQSGAGAA--SSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 409
           NGA+F  SGAG A  SSY++A SL+ARPSS VG IT+ +GAL+C+RGS C
Sbjct: 386 NGAFFTYSGAGPAKSSSYSKASSLAARPSSHVGEITIASGALSCKRGSHC 432

BLAST of Lcy09g005170 vs. ExPASy TrEMBL
Match: A0A6J1BYH1 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111006418 PE=3 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 4.6e-225
Identity = 376/411 (91.48%), Postives = 396/411 (96.35%), Query Frame = 0

Query: 1   MAASLCCFLLLFSVSFLL--TAPTSASSLAD-VQDPHLVVQEVQRSINSSRRNLGYFSCG 60
           MAASL CFLLL S+S LL   AP SASSL + VQDPH VVQEV+RSIN SRRNLGYFSCG
Sbjct: 1   MAASL-CFLLLISLSLLLWSAAPASASSLPNSVQDPHRVVQEVERSINVSRRNLGYFSCG 60

Query: 61  TGNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDSGDDDPLNPRPGTL 120
           TGNPIDDCWRCDPNWE+NRKRLADCAIGFGKNA+GGRNGRFYVV+D+GDDD +NPRPGTL
Sbjct: 61  TGNPIDDCWRCDPNWESNRKRLADCAIGFGKNAMGGRNGRFYVVSDAGDDDAVNPRPGTL 120

Query: 121 RHAVIQNEALWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIILH 180
           RHAVIQNE LWIIFKRDMVI+L+QELVMNS+KTIDGRGASVHVAGGPCITIHYA+NII+H
Sbjct: 121 RHAVIQNEPLWIIFKRDMVIRLKQELVMNSYKTIDGRGASVHVAGGPCITIHYASNIIIH 180

Query: 181 GIHIHDCKRGGNGNIRDSPQHSGWWTVSDGDGVSIFGGRHIWVDHCSLSNCNDGLIDAIH 240
           GIHIHDCKRGGNGNIRDSPQHSGWWTVSDGDGVSIFGG+HIWVDHCSLSNCNDGLIDAIH
Sbjct: 181 GIHIHDCKRGGNGNIRDSPQHSGWWTVSDGDGVSIFGGKHIWVDHCSLSNCNDGLIDAIH 240

Query: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300
           GSTAITISNNYMTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHV
Sbjct: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300

Query: 301 VNNDYTHWEMYAIGGSASPTIYSQGNRFLAPDNRFRKQVTKHEDAPESEWRHWNWRSEGD 360
           VNNDYTHWEMYAIGGSASPTIYSQGNRF APDNRFRK+VTKHEDAPE++WRHWNWRSEGD
Sbjct: 301 VNNDYTHWEMYAIGGSASPTIYSQGNRFAAPDNRFRKEVTKHEDAPETQWRHWNWRSEGD 360

Query: 361 MMLNGAYFLQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 409
           ++LNGAYF QSGAGAASSYARAYSLSARPSSLVGSIT+TAGALNCRRGSRC
Sbjct: 361 LLLNGAYFRQSGAGAASSYARAYSLSARPSSLVGSITLTAGALNCRRGSRC 410

BLAST of Lcy09g005170 vs. ExPASy TrEMBL
Match: A0A6J1H707 (Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111460762 PE=3 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 5.1e-224
Identity = 380/410 (92.68%), Postives = 389/410 (94.88%), Query Frame = 0

Query: 1   MAASLCCFLLLFSVSFLLTAPTSASSLADVQDPHLVVQEVQRSINSS--RRNLGYFSCGT 60
           MAAS   FLLL  +SF  T     SSLA VQDPHLVVQEVQRSIN S  RRNLGY SC T
Sbjct: 53  MAASF-FFLLLLPLSFFFT----DSSLASVQDPHLVVQEVQRSINESRTRRNLGYLSCRT 112

Query: 61  GNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDSGDDDPLNPRPGTLR 120
           GNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTD+GDDDPLNPRPGTLR
Sbjct: 113 GNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDAGDDDPLNPRPGTLR 172

Query: 121 HAVIQNEALWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIILHG 180
           HAVIQNE LWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNII+HG
Sbjct: 173 HAVIQNEPLWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIIIHG 232

Query: 181 IHIHDCKRGGNGNIRDSPQHSGWWTVSDGDGVSIFGGRHIWVDHCSLSNCNDGLIDAIHG 240
           IHIHDCKRGGNGNIRDSPQHSGWWT+SDGDGVSIFGG++IWVDHCSLSNCNDGLIDAIHG
Sbjct: 233 IHIHDCKRGGNGNIRDSPQHSGWWTISDGDGVSIFGGKNIWVDHCSLSNCNDGLIDAIHG 292

Query: 241 STAITISNNYMTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHVV 300
           STAITISNNYMTHHDKVMLLGHSDSYTQDK+MQVTIAFNHFGEGLVQRMPRCRHGYFHVV
Sbjct: 293 STAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVV 352

Query: 301 NNDYTHWEMYAIGGSASPTIYSQGNRFLAPDNRFRKQVTKHEDAPESEWRHWNWRSEGDM 360
           NNDYTHWEMYAIGGSASPTIYSQGNRFLAPD R+RK+VTKHEDAPESEWRHWNWRSEGDM
Sbjct: 353 NNDYTHWEMYAIGGSASPTIYSQGNRFLAPDVRYRKEVTKHEDAPESEWRHWNWRSEGDM 412

Query: 361 MLNGAYFLQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 409
           MLNGAYF QSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC
Sbjct: 413 MLNGAYFRQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 457

BLAST of Lcy09g005170 vs. ExPASy TrEMBL
Match: A0A6J1JPY5 (Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111487839 PE=3 SV=1)

HSP 1 Score: 782.7 bits (2020), Expect = 7.4e-223
Identity = 377/410 (91.95%), Postives = 389/410 (94.88%), Query Frame = 0

Query: 1   MAASLCCFLLLFSVSFLLTAPTSASSLADVQDPHLVVQEVQRSINSS--RRNLGYFSCGT 60
           MAAS   FLLL  +SF  T     SSLA VQDPHLVVQ+VQRSIN S  RRNLGY SC T
Sbjct: 19  MAASF-FFLLLLPLSFFFT----DSSLASVQDPHLVVQQVQRSINESRIRRNLGYLSCRT 78

Query: 61  GNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDSGDDDPLNPRPGTLR 120
           GNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFY+VTD+GDDDPLNPRPGTLR
Sbjct: 79  GNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYMVTDAGDDDPLNPRPGTLR 138

Query: 121 HAVIQNEALWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIILHG 180
           HAVIQNE LWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNII+HG
Sbjct: 139 HAVIQNEPLWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIIIHG 198

Query: 181 IHIHDCKRGGNGNIRDSPQHSGWWTVSDGDGVSIFGGRHIWVDHCSLSNCNDGLIDAIHG 240
           IHIHDCKRGGNGNIRDSPQHSGWWT+SDGDGVSIFGG++IWVDHCSLSNCNDGLIDAIHG
Sbjct: 199 IHIHDCKRGGNGNIRDSPQHSGWWTISDGDGVSIFGGKNIWVDHCSLSNCNDGLIDAIHG 258

Query: 241 STAITISNNYMTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHVV 300
           STAITISNNYMTHHDKVMLLGHSDSYTQDK+MQVTIAFNHFGEGLVQRMPRCRHGYFHVV
Sbjct: 259 STAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVV 318

Query: 301 NNDYTHWEMYAIGGSASPTIYSQGNRFLAPDNRFRKQVTKHEDAPESEWRHWNWRSEGDM 360
           NNDYTHWEMYAIGGSASPTIYSQGNRFLAPD R++K+VTKHEDAPESEWRHWNWRSEGDM
Sbjct: 319 NNDYTHWEMYAIGGSASPTIYSQGNRFLAPDVRYQKEVTKHEDAPESEWRHWNWRSEGDM 378

Query: 361 MLNGAYFLQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 409
           MLNGAYF QSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC
Sbjct: 379 MLNGAYFRQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 423

BLAST of Lcy09g005170 vs. ExPASy TrEMBL
Match: A0A0A0KNX3 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_5G517820 PE=3 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 5.1e-216
Identity = 364/411 (88.56%), Postives = 384/411 (93.43%), Query Frame = 0

Query: 2   AASLCCFLLLFSVSFLL--TAP-TSASSLADV-QDPHLVVQEVQRSINSSRRNLGYFSCG 61
           ++SL   LLL S+SF L  TAP  S SSL  V Q PHLVV+EVQRSIN SRRNLGY SCG
Sbjct: 8   SSSLYFLLLLLSLSFFLSMTAPLPSNSSLPFVHQHPHLVVEEVQRSINGSRRNLGYLSCG 67

Query: 62  TGNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDSGDDDPLNPRPGTL 121
           TGNPIDDCWRCD NWETNRKRLADCAIGFGKNAIGG+NGRFYVVTDSG+DDPLNPRPGTL
Sbjct: 68  TGNPIDDCWRCDSNWETNRKRLADCAIGFGKNAIGGKNGRFYVVTDSGNDDPLNPRPGTL 127

Query: 122 RHAVIQNEALWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIILH 181
           RHAVIQNE LWIIFKRDMVI+L QELVMNSFKTIDGRGASVH+AGGPCI IHYATNII+H
Sbjct: 128 RHAVIQNEPLWIIFKRDMVIKLAQELVMNSFKTIDGRGASVHLAGGPCIKIHYATNIIIH 187

Query: 182 GIHIHDCKRGGNGNIRDSPQHSGWWTVSDGDGVSIFGGRHIWVDHCSLSNCNDGLIDAIH 241
           GIHIHDCKRGGN NIRDSPQH+GWWT SDGDGVSI GG+ IW+DHCSLSNCNDGLIDAIH
Sbjct: 188 GIHIHDCKRGGNANIRDSPQHAGWWTASDGDGVSILGGKQIWIDHCSLSNCNDGLIDAIH 247

Query: 242 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHV 301
           GSTAITISNNYMTHHDKVMLLGHSDS+TQDK+MQVTIAFNHFGEGLVQRMPRCRHGYFHV
Sbjct: 248 GSTAITISNNYMTHHDKVMLLGHSDSFTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHV 307

Query: 302 VNNDYTHWEMYAIGGSASPTIYSQGNRFLAPDNRFRKQVTKHEDAPESEWRHWNWRSEGD 361
           VNNDYTHWEMYAIGGSASPTIYSQGNRF+AP NRFRK+VTKHEDAPESEWRHWNWRSEGD
Sbjct: 308 VNNDYTHWEMYAIGGSASPTIYSQGNRFVAPYNRFRKEVTKHEDAPESEWRHWNWRSEGD 367

Query: 362 MMLNGAYFLQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 409
           ++LNGAYF QSGAGA+SSYARAYSLSARPSSLVG IT+TAGALNCRRGSRC
Sbjct: 368 LLLNGAYFRQSGAGASSSYARAYSLSARPSSLVGDITITAGALNCRRGSRC 418

BLAST of Lcy09g005170 vs. ExPASy TrEMBL
Match: A0A5D3CGC7 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold249G00560 PE=3 SV=1)

HSP 1 Score: 755.4 bits (1949), Expect = 1.3e-214
Identity = 357/392 (91.07%), Postives = 370/392 (94.39%), Query Frame = 0

Query: 19  TAP-TSASSLADV-QDPHLVVQEVQRSINSSRRNLGYFSCGTGNPIDDCWRCDPNWETNR 78
           TAP  S SSL  V Q PHLVV+EVQRSIN SRRNLGY SCGTGNPIDDCWRCD NWETNR
Sbjct: 3   TAPIPSNSSLPFVHQHPHLVVEEVQRSINGSRRNLGYLSCGTGNPIDDCWRCDSNWETNR 62

Query: 79  KRLADCAIGFGKNAIGGRNGRFYVVTDSGDDDPLNPRPGTLRHAVIQNEALWIIFKRDMV 138
           KRLADCAIGFGKNAIGG+NGRFYVVTD+ DDDPLNPRPGTLRHAVIQNE LWIIFKRDMV
Sbjct: 63  KRLADCAIGFGKNAIGGKNGRFYVVTDARDDDPLNPRPGTLRHAVIQNEPLWIIFKRDMV 122

Query: 139 IQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIILHGIHIHDCKRGGNGNIRDSP 198
           IQL QELVMNSFKTIDGRGASVHVAGGPCI IHYATNII+HGIHIHDCKRGGN NIRDSP
Sbjct: 123 IQLEQELVMNSFKTIDGRGASVHVAGGPCIKIHYATNIIIHGIHIHDCKRGGNANIRDSP 182

Query: 199 QHSGWWTVSDGDGVSIFGGRHIWVDHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVM 258
           QH+GWWT SDGDGVSI GG+ IW+DHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVM
Sbjct: 183 QHAGWWTASDGDGVSILGGKQIWIDHCSLSNCNDGLIDAIHGSTAITISNNYMTHHDKVM 242

Query: 259 LLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASP 318
           LLGHSDS+TQDK+MQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASP
Sbjct: 243 LLGHSDSFTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSASP 302

Query: 319 TIYSQGNRFLAPDNRFRKQVTKHEDAPESEWRHWNWRSEGDMMLNGAYFLQSGAGAASSY 378
           TIYSQGNRF+AP NRFRK+VTKHEDAPESEWRHWNWRSEGDM+LNGAYF QSGAGA SSY
Sbjct: 303 TIYSQGNRFVAPYNRFRKEVTKHEDAPESEWRHWNWRSEGDMLLNGAYFRQSGAGARSSY 362

Query: 379 ARAYSLSARPSSLVGSITVTAGALNCRRGSRC 409
           ARAYSLSARPSSLVG IT+TAGALNCRRGSRC
Sbjct: 363 ARAYSLSARPSSLVGDITLTAGALNCRRGSRC 394

BLAST of Lcy09g005170 vs. NCBI nr
Match: XP_022134052.1 (pectate lyase-like [Momordica charantia])

HSP 1 Score: 790.0 bits (2039), Expect = 9.5e-225
Identity = 376/411 (91.48%), Postives = 396/411 (96.35%), Query Frame = 0

Query: 1   MAASLCCFLLLFSVSFLL--TAPTSASSLAD-VQDPHLVVQEVQRSINSSRRNLGYFSCG 60
           MAASL CFLLL S+S LL   AP SASSL + VQDPH VVQEV+RSIN SRRNLGYFSCG
Sbjct: 1   MAASL-CFLLLISLSLLLWSAAPASASSLPNSVQDPHRVVQEVERSINVSRRNLGYFSCG 60

Query: 61  TGNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDSGDDDPLNPRPGTL 120
           TGNPIDDCWRCDPNWE+NRKRLADCAIGFGKNA+GGRNGRFYVV+D+GDDD +NPRPGTL
Sbjct: 61  TGNPIDDCWRCDPNWESNRKRLADCAIGFGKNAMGGRNGRFYVVSDAGDDDAVNPRPGTL 120

Query: 121 RHAVIQNEALWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIILH 180
           RHAVIQNE LWIIFKRDMVI+L+QELVMNS+KTIDGRGASVHVAGGPCITIHYA+NII+H
Sbjct: 121 RHAVIQNEPLWIIFKRDMVIRLKQELVMNSYKTIDGRGASVHVAGGPCITIHYASNIIIH 180

Query: 181 GIHIHDCKRGGNGNIRDSPQHSGWWTVSDGDGVSIFGGRHIWVDHCSLSNCNDGLIDAIH 240
           GIHIHDCKRGGNGNIRDSPQHSGWWTVSDGDGVSIFGG+HIWVDHCSLSNCNDGLIDAIH
Sbjct: 181 GIHIHDCKRGGNGNIRDSPQHSGWWTVSDGDGVSIFGGKHIWVDHCSLSNCNDGLIDAIH 240

Query: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300
           GSTAITISNNYMTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHV
Sbjct: 241 GSTAITISNNYMTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHV 300

Query: 301 VNNDYTHWEMYAIGGSASPTIYSQGNRFLAPDNRFRKQVTKHEDAPESEWRHWNWRSEGD 360
           VNNDYTHWEMYAIGGSASPTIYSQGNRF APDNRFRK+VTKHEDAPE++WRHWNWRSEGD
Sbjct: 301 VNNDYTHWEMYAIGGSASPTIYSQGNRFAAPDNRFRKEVTKHEDAPETQWRHWNWRSEGD 360

Query: 361 MMLNGAYFLQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 409
           ++LNGAYF QSGAGAASSYARAYSLSARPSSLVGSIT+TAGALNCRRGSRC
Sbjct: 361 LLLNGAYFRQSGAGAASSYARAYSLSARPSSLVGSITLTAGALNCRRGSRC 410

BLAST of Lcy09g005170 vs. NCBI nr
Match: XP_023526071.1 (pectate lyase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 790.0 bits (2039), Expect = 9.5e-225
Identity = 381/410 (92.93%), Postives = 390/410 (95.12%), Query Frame = 0

Query: 1   MAASLCCFLLLFSVSFLLTAPTSASSLADVQDPHLVVQEVQRSINSS--RRNLGYFSCGT 60
           MAAS   FLLL  +SF  T     SSLA+VQDPHLVVQEVQRSIN S  RRNLGY SC T
Sbjct: 1   MAASF-FFLLLLPLSFFFT----DSSLAEVQDPHLVVQEVQRSINESRTRRNLGYLSCRT 60

Query: 61  GNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDSGDDDPLNPRPGTLR 120
           GNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTD+GDDDPLNPRPGTLR
Sbjct: 61  GNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDAGDDDPLNPRPGTLR 120

Query: 121 HAVIQNEALWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIILHG 180
           HAVIQNE LWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNII+HG
Sbjct: 121 HAVIQNEPLWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIIIHG 180

Query: 181 IHIHDCKRGGNGNIRDSPQHSGWWTVSDGDGVSIFGGRHIWVDHCSLSNCNDGLIDAIHG 240
           IHIHDCKRGGNGNIRDSPQHSGWWT+SDGDGVSIFGG+HIWVDHCSLSNCNDGLIDAIHG
Sbjct: 181 IHIHDCKRGGNGNIRDSPQHSGWWTISDGDGVSIFGGKHIWVDHCSLSNCNDGLIDAIHG 240

Query: 241 STAITISNNYMTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHVV 300
           STAITISNNYMTHHDKVMLLGHSDSYTQDK+MQVTIAFNHFGEGLVQRMPRCRHGYFHVV
Sbjct: 241 STAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVV 300

Query: 301 NNDYTHWEMYAIGGSASPTIYSQGNRFLAPDNRFRKQVTKHEDAPESEWRHWNWRSEGDM 360
           NNDYTHWEMYAIGGSASPTIYSQGNRFLAPD R+RK+VTKHEDAPESEWRHWNWRSEGDM
Sbjct: 301 NNDYTHWEMYAIGGSASPTIYSQGNRFLAPDVRYRKEVTKHEDAPESEWRHWNWRSEGDM 360

Query: 361 MLNGAYFLQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 409
           MLNGAYF QSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC
Sbjct: 361 MLNGAYFRQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 405

BLAST of Lcy09g005170 vs. NCBI nr
Match: XP_022959813.1 (pectate lyase-like [Cucurbita moschata])

HSP 1 Score: 786.6 bits (2030), Expect = 1.1e-223
Identity = 380/410 (92.68%), Postives = 389/410 (94.88%), Query Frame = 0

Query: 1   MAASLCCFLLLFSVSFLLTAPTSASSLADVQDPHLVVQEVQRSINSS--RRNLGYFSCGT 60
           MAAS   FLLL  +SF  T     SSLA VQDPHLVVQEVQRSIN S  RRNLGY SC T
Sbjct: 53  MAASF-FFLLLLPLSFFFT----DSSLASVQDPHLVVQEVQRSINESRTRRNLGYLSCRT 112

Query: 61  GNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDSGDDDPLNPRPGTLR 120
           GNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTD+GDDDPLNPRPGTLR
Sbjct: 113 GNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDAGDDDPLNPRPGTLR 172

Query: 121 HAVIQNEALWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIILHG 180
           HAVIQNE LWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNII+HG
Sbjct: 173 HAVIQNEPLWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIIIHG 232

Query: 181 IHIHDCKRGGNGNIRDSPQHSGWWTVSDGDGVSIFGGRHIWVDHCSLSNCNDGLIDAIHG 240
           IHIHDCKRGGNGNIRDSPQHSGWWT+SDGDGVSIFGG++IWVDHCSLSNCNDGLIDAIHG
Sbjct: 233 IHIHDCKRGGNGNIRDSPQHSGWWTISDGDGVSIFGGKNIWVDHCSLSNCNDGLIDAIHG 292

Query: 241 STAITISNNYMTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHVV 300
           STAITISNNYMTHHDKVMLLGHSDSYTQDK+MQVTIAFNHFGEGLVQRMPRCRHGYFHVV
Sbjct: 293 STAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVV 352

Query: 301 NNDYTHWEMYAIGGSASPTIYSQGNRFLAPDNRFRKQVTKHEDAPESEWRHWNWRSEGDM 360
           NNDYTHWEMYAIGGSASPTIYSQGNRFLAPD R+RK+VTKHEDAPESEWRHWNWRSEGDM
Sbjct: 353 NNDYTHWEMYAIGGSASPTIYSQGNRFLAPDVRYRKEVTKHEDAPESEWRHWNWRSEGDM 412

Query: 361 MLNGAYFLQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 409
           MLNGAYF QSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC
Sbjct: 413 MLNGAYFRQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 457

BLAST of Lcy09g005170 vs. NCBI nr
Match: KAG6602397.1 (putative pectate lyase 11, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033073.1 putative pectate lyase 11, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 784.6 bits (2025), Expect = 4.0e-223
Identity = 375/403 (93.05%), Postives = 384/403 (95.29%), Query Frame = 0

Query: 8   FLLLFSVSFLLTAPTSASSLADVQDPHLVVQEVQRSINSS--RRNLGYFSCGTGNPIDDC 67
           FLLL  +SF        SSLA VQDPHLVVQEVQRSIN S  RRNLGY SC TGNPIDDC
Sbjct: 7   FLLLLPLSFFFI----DSSLASVQDPHLVVQEVQRSINESRTRRNLGYLSCRTGNPIDDC 66

Query: 68  WRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDSGDDDPLNPRPGTLRHAVIQNE 127
           WRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTD+GDDDPLNPRPGTLRHAVIQNE
Sbjct: 67  WRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDAGDDDPLNPRPGTLRHAVIQNE 126

Query: 128 ALWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIILHGIHIHDCK 187
            LWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNII+HGIHIHDCK
Sbjct: 127 PLWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIIIHGIHIHDCK 186

Query: 188 RGGNGNIRDSPQHSGWWTVSDGDGVSIFGGRHIWVDHCSLSNCNDGLIDAIHGSTAITIS 247
           RGGNGNIRDSPQHSGWWT+SDGDGVSIFGG++IWVDHCSLSNCNDGLIDAIHGSTAITIS
Sbjct: 187 RGGNGNIRDSPQHSGWWTISDGDGVSIFGGKNIWVDHCSLSNCNDGLIDAIHGSTAITIS 246

Query: 248 NNYMTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 307
           NNYMTHHDKVMLLGHSDSYTQDK+MQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW
Sbjct: 247 NNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 306

Query: 308 EMYAIGGSASPTIYSQGNRFLAPDNRFRKQVTKHEDAPESEWRHWNWRSEGDMMLNGAYF 367
           EMYAIGGSASPTIYSQGNRFLAPD R+RK+VTKHEDAPESEWRHWNWRSEGDMMLNGAYF
Sbjct: 307 EMYAIGGSASPTIYSQGNRFLAPDVRYRKEVTKHEDAPESEWRHWNWRSEGDMMLNGAYF 366

Query: 368 LQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 409
            QSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC
Sbjct: 367 RQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 405

BLAST of Lcy09g005170 vs. NCBI nr
Match: XP_022991156.1 (pectate lyase-like, partial [Cucurbita maxima])

HSP 1 Score: 782.7 bits (2020), Expect = 1.5e-222
Identity = 377/410 (91.95%), Postives = 389/410 (94.88%), Query Frame = 0

Query: 1   MAASLCCFLLLFSVSFLLTAPTSASSLADVQDPHLVVQEVQRSINSS--RRNLGYFSCGT 60
           MAAS   FLLL  +SF  T     SSLA VQDPHLVVQ+VQRSIN S  RRNLGY SC T
Sbjct: 19  MAASF-FFLLLLPLSFFFT----DSSLASVQDPHLVVQQVQRSINESRIRRNLGYLSCRT 78

Query: 61  GNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDSGDDDPLNPRPGTLR 120
           GNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFY+VTD+GDDDPLNPRPGTLR
Sbjct: 79  GNPIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYMVTDAGDDDPLNPRPGTLR 138

Query: 121 HAVIQNEALWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIILHG 180
           HAVIQNE LWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNII+HG
Sbjct: 139 HAVIQNEPLWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIIIHG 198

Query: 181 IHIHDCKRGGNGNIRDSPQHSGWWTVSDGDGVSIFGGRHIWVDHCSLSNCNDGLIDAIHG 240
           IHIHDCKRGGNGNIRDSPQHSGWWT+SDGDGVSIFGG++IWVDHCSLSNCNDGLIDAIHG
Sbjct: 199 IHIHDCKRGGNGNIRDSPQHSGWWTISDGDGVSIFGGKNIWVDHCSLSNCNDGLIDAIHG 258

Query: 241 STAITISNNYMTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHVV 300
           STAITISNNYMTHHDKVMLLGHSDSYTQDK+MQVTIAFNHFGEGLVQRMPRCRHGYFHVV
Sbjct: 259 STAITISNNYMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVV 318

Query: 301 NNDYTHWEMYAIGGSASPTIYSQGNRFLAPDNRFRKQVTKHEDAPESEWRHWNWRSEGDM 360
           NNDYTHWEMYAIGGSASPTIYSQGNRFLAPD R++K+VTKHEDAPESEWRHWNWRSEGDM
Sbjct: 319 NNDYTHWEMYAIGGSASPTIYSQGNRFLAPDVRYQKEVTKHEDAPESEWRHWNWRSEGDM 378

Query: 361 MLNGAYFLQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 409
           MLNGAYF QSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC
Sbjct: 379 MLNGAYFRQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 423

BLAST of Lcy09g005170 vs. TAIR 10
Match: AT3G27400.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 677.6 bits (1747), Expect = 6.4e-195
Identity = 318/403 (78.91%), Postives = 356/403 (88.34%), Query Frame = 0

Query: 8   FLLLFSVSFLLTAPTSASSLAD-VQDPHLVVQEVQRSI-NSSRRNLGYFSCGTGNPIDDC 67
           FLLL ++   L     +SSL D VQDP+LVV +V RS+ N+SRR+L Y SC TGNPIDDC
Sbjct: 13  FLLLLTIGNTL---AFSSSLPDHVQDPNLVVDDVNRSVFNASRRSLAYLSCRTGNPIDDC 72

Query: 68  WRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDSGDDDPLNPRPGTLRHAVIQNE 127
           WRCDPNWETNR+RLADCAIGFGKNAIGGR GR YVVTD  +DDP+NPRPGTLR+AV Q E
Sbjct: 73  WRCDPNWETNRQRLADCAIGFGKNAIGGRKGRIYVVTDPANDDPVNPRPGTLRYAVTQEE 132

Query: 128 ALWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIILHGIHIHDCK 187
            LWIIFKRDMVI+L++EL++ SFKTIDGRG+SVH+  GPC+ IHYATNII+HGI+IHDCK
Sbjct: 133 PLWIIFKRDMVIRLKKELIITSFKTIDGRGSSVHITDGPCLKIHYATNIIIHGINIHDCK 192

Query: 188 RGGNGNIRDSPQHSGWWTVSDGDGVSIFGGRHIWVDHCSLSNCNDGLIDAIHGSTAITIS 247
            G  G I+D P H+GWW  SDGD V+IFGG+H+W+DHCSLSNC+DGLIDAIHGSTAITIS
Sbjct: 193 PGSGGMIKDGPHHTGWWMQSDGDAVAIFGGKHVWIDHCSLSNCDDGLIDAIHGSTAITIS 252

Query: 248 NNYMTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 307
           NN+MTHHDKVMLLGHSDSYTQDK+MQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW
Sbjct: 253 NNHMTHHDKVMLLGHSDSYTQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 312

Query: 308 EMYAIGGSASPTIYSQGNRFLAPDNRFRKQVTKHEDAPESEWRHWNWRSEGDMMLNGAYF 367
           EMYAIGGSASPTIYSQGNRFLAP+ RF K+VTKHEDAPES+WR WNWRSEGDM+LNGAYF
Sbjct: 313 EMYAIGGSASPTIYSQGNRFLAPNTRFNKEVTKHEDAPESKWRDWNWRSEGDMLLNGAYF 372

Query: 368 LQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 409
            +SGA A S+YARA SLSARPSSLVGSIT TAG L+CRRG RC
Sbjct: 373 RESGAEAPSTYARASSLSARPSSLVGSITTTAGTLSCRRGRRC 412

BLAST of Lcy09g005170 vs. TAIR 10
Match: AT4G24780.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 664.8 bits (1714), Expect = 4.3e-191
Identity = 309/403 (76.67%), Postives = 355/403 (88.09%), Query Frame = 0

Query: 9   LLLFSVSFLLTAPTSASSLADVQDPHLVVQEVQRSINSS---RRNLGYFSCGTGNPIDDC 68
           L +  VSFLL AP   SS   V DP  VV+EV +SIN+S   RR LGY SC TGNPIDDC
Sbjct: 8   LFITIVSFLLYAPLFLSS--PVPDPESVVEEVHKSINASVAGRRKLGYLSCTTGNPIDDC 67

Query: 69  WRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDSGDDDPLNPRPGTLRHAVIQNE 128
           WRCDP+WE +R+RLADCAIGFGKNAIGGR+GR YVVTDSG+D+P++P+PGTLRHAV+Q+E
Sbjct: 68  WRCDPHWEQHRQRLADCAIGFGKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVVQDE 127

Query: 129 ALWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIILHGIHIHDCK 188
            LWIIF+RDM IQL++EL+MNSFKTIDGRGASVH++GGPCITI Y TNII+HGIHIHDCK
Sbjct: 128 PLWIIFQRDMTIQLKEELIMNSFKTIDGRGASVHISGGPCITIQYVTNIIIHGIHIHDCK 187

Query: 189 RGGNGNIRDSPQHSGWWTVSDGDGVSIFGGRHIWVDHCSLSNCNDGLIDAIHGSTAITIS 248
           +GGN  +R SP+H GW T+SDGDGVSIFGG H+WVDHCS SNC DGLIDAI GSTAIT+S
Sbjct: 188 QGGNAMVRSSPRHFGWRTISDGDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAITLS 247

Query: 249 NNYMTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 308
           NN+MTHHDKVMLLGHSD+Y++DK+MQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW
Sbjct: 248 NNHMTHHDKVMLLGHSDTYSRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 307

Query: 309 EMYAIGGSASPTIYSQGNRFLAPDNRFRKQVTKHEDAPESEWRHWNWRSEGDMMLNGAYF 368
           EMYAIGGSA+PTI SQGNRFLAP+ RF K+VTKHEDAPESEW+ WNWRS GD++LNGA+F
Sbjct: 308 EMYAIGGSANPTINSQGNRFLAPNIRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNGAFF 367

Query: 369 LQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 409
             SG  A+SSYA+A SL A+PSSLVG +T T+GALNCR+GSRC
Sbjct: 368 TPSGGAASSSYAKASSLGAKPSSLVGPLTSTSGALNCRKGSRC 408

BLAST of Lcy09g005170 vs. TAIR 10
Match: AT4G24780.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 664.8 bits (1714), Expect = 4.3e-191
Identity = 309/403 (76.67%), Postives = 355/403 (88.09%), Query Frame = 0

Query: 9   LLLFSVSFLLTAPTSASSLADVQDPHLVVQEVQRSINSS---RRNLGYFSCGTGNPIDDC 68
           L +  VSFLL AP   SS   V DP  VV+EV +SIN+S   RR LGY SC TGNPIDDC
Sbjct: 8   LFITIVSFLLYAPLFLSS--PVPDPESVVEEVHKSINASVAGRRKLGYLSCTTGNPIDDC 67

Query: 69  WRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDSGDDDPLNPRPGTLRHAVIQNE 128
           WRCDP+WE +R+RLADCAIGFGKNAIGGR+GR YVVTDSG+D+P++P+PGTLRHAV+Q+E
Sbjct: 68  WRCDPHWEQHRQRLADCAIGFGKNAIGGRDGRIYVVTDSGNDNPVSPKPGTLRHAVVQDE 127

Query: 129 ALWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIILHGIHIHDCK 188
            LWIIF+RDM IQL++EL+MNSFKTIDGRGASVH++GGPCITI Y TNII+HGIHIHDCK
Sbjct: 128 PLWIIFQRDMTIQLKEELIMNSFKTIDGRGASVHISGGPCITIQYVTNIIIHGIHIHDCK 187

Query: 189 RGGNGNIRDSPQHSGWWTVSDGDGVSIFGGRHIWVDHCSLSNCNDGLIDAIHGSTAITIS 248
           +GGN  +R SP+H GW T+SDGDGVSIFGG H+WVDHCS SNC DGLIDAI GSTAIT+S
Sbjct: 188 QGGNAMVRSSPRHFGWRTISDGDGVSIFGGSHVWVDHCSFSNCEDGLIDAIMGSTAITLS 247

Query: 249 NNYMTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 308
           NN+MTHHDKVMLLGHSD+Y++DK+MQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW
Sbjct: 248 NNHMTHHDKVMLLGHSDTYSRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW 307

Query: 309 EMYAIGGSASPTIYSQGNRFLAPDNRFRKQVTKHEDAPESEWRHWNWRSEGDMMLNGAYF 368
           EMYAIGGSA+PTI SQGNRFLAP+ RF K+VTKHEDAPESEW+ WNWRS GD++LNGA+F
Sbjct: 308 EMYAIGGSANPTINSQGNRFLAPNIRFSKEVTKHEDAPESEWKRWNWRSSGDLLLNGAFF 367

Query: 369 LQSGAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 409
             SG  A+SSYA+A SL A+PSSLVG +T T+GALNCR+GSRC
Sbjct: 368 TPSGGAASSSYAKASSLGAKPSSLVGPLTSTSGALNCRKGSRC 408

BLAST of Lcy09g005170 vs. TAIR 10
Match: AT5G63180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 658.3 bits (1697), Expect = 4.0e-189
Identity = 313/410 (76.34%), Postives = 356/410 (86.83%), Query Frame = 0

Query: 3   ASLCCFLLLFSVSFLLTAPTSASSLADVQDPHLVVQEVQRSINS--SRRNLGYFSCGTGN 62
           +S   F L+F    L +A   ASSL  V DP LVV+EV R IN   SRR LG+FSCG+GN
Sbjct: 26  SSYLSFALIFFCCILFSA--LASSL-PVSDPELVVEEVHRKINESISRRKLGFFSCGSGN 85

Query: 63  PIDDCWRCDPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDSGDDDPLNPRPGTLRHA 122
           PIDDCWRCD +WE NRKRLADC IGFGKNAIGGR+G  YVVTD G+DDP+NPRPGTLR+A
Sbjct: 86  PIDDCWRCDKDWEKNRKRLADCGIGFGKNAIGGRDGEIYVVTDPGNDDPVNPRPGTLRYA 145

Query: 123 VIQNEALWIIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIILHGIH 182
           VIQ+E LWIIFKRDM IQL++EL+MNSFKT+DGRGASVH++GGPCITI Y TNII+HG+H
Sbjct: 146 VIQDEPLWIIFKRDMTIQLKEELIMNSFKTLDGRGASVHISGGPCITIQYVTNIIIHGLH 205

Query: 183 IHDCKRGGNGNIRDSPQHSGWWTVSDGDGVSIFGGRHIWVDHCSLSNCNDGLIDAIHGST 242
           IHDCK+GGN  +RDSP+H G+ TVSDGDGVSIFGG H+WVDHCSLSNCNDGLIDAI GST
Sbjct: 206 IHDCKQGGNTYVRDSPEHYGYRTVSDGDGVSIFGGSHVWVDHCSLSNCNDGLIDAIRGST 265

Query: 243 AITISNNYMTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN 302
           AITISNNY+THH+KVMLLGHSD+Y QDK+MQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN
Sbjct: 266 AITISNNYLTHHNKVMLLGHSDTYEQDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNN 325

Query: 303 DYTHWEMYAIGGSASPTIYSQGNRFLAPDNRFRKQVTKHEDAPESEWRHWNWRSEGDMML 362
           DYTHWEMYAIGGSA+PTI SQGNRFLAPD+   K+VTKHEDAPE EWR+WNWRSEGD++L
Sbjct: 326 DYTHWEMYAIGGSANPTINSQGNRFLAPDDSSSKEVTKHEDAPEDEWRNWNWRSEGDLLL 385

Query: 363 NGAYFLQSGAGAA--SSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 409
           NGA+F  SGAG A  SSY++A SL+ARPSS VG IT+ +GAL+C+RGS C
Sbjct: 386 NGAFFTYSGAGPAKSSSYSKASSLAARPSSHVGEITIASGALSCKRGSHC 432

BLAST of Lcy09g005170 vs. TAIR 10
Match: AT1G67750.1 (Pectate lyase family protein )

HSP 1 Score: 654.1 bits (1686), Expect = 7.6e-188
Identity = 305/400 (76.25%), Postives = 347/400 (86.75%), Query Frame = 0

Query: 9   LLLFSVSFLLTAPTSASSLADVQDPHLVVQEVQRSINSSRRNLGYFSCGTGNPIDDCWRC 68
           L LFS   L+ +PT  +S   V +P LVVQEV   IN+SRRNLG  SCGTGNPIDDCWRC
Sbjct: 10  LSLFSCLLLVLSPTFIAS-TPVSEPELVVQEVNEKINASRRNLGVLSCGTGNPIDDCWRC 69

Query: 69  DPNWETNRKRLADCAIGFGKNAIGGRNGRFYVVTDSGDDDPLNPRPGTLRHAVIQNEALW 128
           DP WE NR+RLADCAIGFGK+AIGGR+G+ YVVTDS D D +NP+PGTLRHAVIQ+E LW
Sbjct: 70  DPKWEKNRQRLADCAIGFGKHAIGGRDGKIYVVTDSSDKDVVNPKPGTLRHAVIQDEPLW 129

Query: 129 IIFKRDMVIQLRQELVMNSFKTIDGRGASVHVAGGPCITIHYATNIILHGIHIHDCKRGG 188
           IIF RDMVI+L++EL+MNSFKTIDGRGASVH+AGG CIT+ Y TNII+HG++IHDCKR G
Sbjct: 130 IIFARDMVIKLKEELIMNSFKTIDGRGASVHIAGGACITVQYVTNIIIHGVNIHDCKRKG 189

Query: 189 NGNIRDSPQHSGWWTVSDGDGVSIFGGRHIWVDHCSLSNCNDGLIDAIHGSTAITISNNY 248
           N  +RDSP H GW T SDGD VSIFGG H+WVDHCSLSNC DGLIDAIHGSTAITISNNY
Sbjct: 190 NAYVRDSPSHYGWRTASDGDAVSIFGGSHVWVDHCSLSNCADGLIDAIHGSTAITISNNY 249

Query: 249 MTHHDKVMLLGHSDSYTQDKDMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMY 308
           ++HH+KVMLLGHSDSYT+DK+MQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHW+MY
Sbjct: 250 LSHHNKVMLLGHSDSYTRDKNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWQMY 309

Query: 309 AIGGSASPTIYSQGNRFLAPDNRFRKQVTKHEDAPESEWRHWNWRSEGDMMLNGAYFLQS 368
           AIGGSA+PTI SQGNRFLAP++   K+VTK+EDAP S+W+ WNWRSEGD+ LNGA+F  S
Sbjct: 310 AIGGSAAPTINSQGNRFLAPNDHVFKEVTKYEDAPRSKWKKWNWRSEGDLFLNGAFFTPS 369

Query: 369 GAGAASSYARAYSLSARPSSLVGSITVTAGALNCRRGSRC 409
           G GA+SSYA+A SLSARPSSLV S+T  AGAL CR+GSRC
Sbjct: 370 GGGASSSYAKASSLSARPSSLVASVTSNAGALFCRKGSRC 408

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O245549.7e-19679.95Pectate lyase OS=Zinnia violacea OX=34245 PE=1 SV=1[more]
Q9LTZ09.0e-19478.91Putative pectate lyase 11 OS=Arabidopsis thaliana OX=3702 GN=At3g27400 PE=3 SV=2[more]
Q9C5M86.1e-19076.67Probable pectate lyase 18 OS=Arabidopsis thaliana OX=3702 GN=At4g24780 PE=2 SV=2[more]
P243962.3e-18977.92Probable pectate lyase P18 OS=Solanum lycopersicum OX=4081 GN=9612 PE=2 SV=1[more]
Q93Z255.7e-18876.34Probable pectate lyase 22 OS=Arabidopsis thaliana OX=3702 GN=At5g63180 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1BYH14.6e-22591.48Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111006418 PE=3 SV=1[more]
A0A6J1H7075.1e-22492.68Pectate lyase OS=Cucurbita moschata OX=3662 GN=LOC111460762 PE=3 SV=1[more]
A0A6J1JPY57.4e-22391.95Pectate lyase OS=Cucurbita maxima OX=3661 GN=LOC111487839 PE=3 SV=1[more]
A0A0A0KNX35.1e-21688.56Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_5G517820 PE=3 SV=1[more]
A0A5D3CGC71.3e-21491.07Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold249G00560 ... [more]
Match NameE-valueIdentityDescription
XP_022134052.19.5e-22591.48pectate lyase-like [Momordica charantia][more]
XP_023526071.19.5e-22592.93pectate lyase-like [Cucurbita pepo subsp. pepo][more]
XP_022959813.11.1e-22392.68pectate lyase-like [Cucurbita moschata][more]
KAG6602397.14.0e-22393.05putative pectate lyase 11, partial [Cucurbita argyrosperma subsp. sororia] >KAG7... [more]
XP_022991156.11.5e-22291.95pectate lyase-like, partial [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G27400.16.4e-19578.91Pectin lyase-like superfamily protein [more]
AT4G24780.14.3e-19176.67Pectin lyase-like superfamily protein [more]
AT4G24780.24.3e-19176.67Pectin lyase-like superfamily protein [more]
AT5G63180.14.0e-18976.34Pectin lyase-like superfamily protein [more]
AT1G67750.17.6e-18876.25Pectate lyase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 286..305
score: 78.1
coord: 308..327
score: 78.33
coord: 82..99
score: 58.99
coord: 106..131
score: 65.02
coord: 206..227
score: 56.28
coord: 350..374
score: 53.33
coord: 379..402
score: 40.67
coord: 142..158
score: 71.43
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 134..331
e-value: 1.8E-104
score: 363.0
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 140..325
e-value: 1.1E-20
score: 74.1
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 59..401
e-value: 1.2E-116
score: 391.5
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 9..405
NoneNo IPR availablePANTHERPTHR31683:SF76PECTATE LYASEcoord: 9..405
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 44..403

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g005170.1Lcy09g005170.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0046872 metal ion binding
molecular_function GO:0030570 pectate lyase activity