Lcy09g004180 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g004180
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionMethyltransferase
LocationChr09: 3734352 .. 3739154 (+)
RNA-Seq ExpressionLcy09g004180
SyntenyLcy09g004180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCCGTTGATGGCGGTTTCTTCGTTCCCGGTTCTTTCTTCATTGAGTGCCAGTGTTAATATCTCTTCGGATGTTAGCTTGTTTTCTCTGAATCTTCCACTCGTCAATTTGTTTTACAGATCGAGCACGCATCCGTCGTCTTCTACTATCGTTTGCTCGTACAGTGAGTTTCTGTTATGTTATGTCGGAGAAATGGAAAATGATTTTGTTTTATCCTTTATTTTGCTATAATTGAACTTTTGATTGTGTACTGGTTTCAGAATCTGGGAATGATACGGTGACGGAGCGGAGGAAAAAGTTGTTGGAGCAATATGGACTTGATCCTGACGAGTTTCTGTCTGAATCTTCTACTAATGTTGGTTCTCTCTCTCTCTCCGATGGTTGTTTTCTTTCATTTATTTTCAAAGTGTTTTTGAGATGAGTGACTGAAATTTTATTATTATTTGCCGTTTGAATAATGCAATCGCATTATATTGGCTACGGAATTGAGTGCTTGGATATTTGGTAAGCTTAATTTTCTTCGCTCGAGTTGCTCTATTAATCTCTCTGATCGTTAATTTGACTTATAGAGAAAAAGAGAAGTGCTAAAATCGGGAAAAGGCAAGCAGGGTTCAGCCGAGGATCCGAAACCTCAACGGCAGACCCATAAGTTACTTCAGGTACGACCTTTATTCCTTTTTCCATTTTGCTTAGTGCTTTGTGCTGGGAGAAAGATGGGGTAGACTCGAGTTGTGATAGCGTGACATTGATATCAAATTTTCAATTCATTCTATCCTCAGAAGTGACCAACATTCTCTGGCATAACTCGGCGCTACTATCCCCACTATTTATCTTCCAAAGTACAGAAGGGATATGGCTCTCTGCCGTAGAGATTAGTCCTTCAAAATTTCGAAATGAAGTGAAAGGGGAGAGAAATGTTTGTGTAATTTGATATTGAAGGTCTTTTGGTAATAGAACTTTTCTTACAAGGATGGATATAAACAAACGCTCTATTTGGCTTAAAGATATTCCATCTTAAATACCCAATTACCCAAATCGAAAACTATAGTATTAGTTCAATTCTGTAAGTTGATTGAATCATTTCATTTTGGATTTATTCCAGTCACCAAGTTAATCATTTTCAATAGCCCAATTTGCCATATTGAAAGCTAAAATACTAATTTAGTTATTTAGTCTGAGACAATATTATGTCGAGAATCTATTACGTTGATGATACCCATGGATTCTCCAATTCCTACACATCACGTGTACCACAGATATGGAAACATATTTATTAGATTCCTTATTTTTATTTTTTAAAAAATCAATCAATTTGTTGGCAACCTCAGGTGCTTGGAGGTAGTGCTCGGAGAATGAAGCTACTCTCACCAAAGGGCTTGGATGTACGACCGATGATGGAAGTTGTGAAAGGTGCAGCTTTTGATATTTTGCAGGTTGGAGTTGTATGTTAAATATGAGTTTCTTTCACGTTACCAGATTATTCTGCTAAGTGCTAACTTATCAAATGATTCAGAGTTTGTAGATTTCTAGTTCAAGAGTTCATTCAGTAGCAAAACATTTTCTATATTCATACTTTTCTTGGGCAGGTTGCTGGTAGCTATCCTGTCACAAAAAGGAAAGGTCTAATGTAATTTGAAAATGCAGGCTGCTGGTGGCTGTCCAGCATTTTTAAGGCCTGGTCGTTGGTTAGACTTGTATAGTGGTACTGGATCAGTTGGTATTGAAGCTATGAGCCGTGGATGTTCTGAGGTACTAGATCAATTGAGAATTTAGTTTGGGATAACATTGTCCATTATTTTAGAAATAACTAACTGATAAAGCAGCTTTCGAACTATGCGTAGATAGGGATTGGCTTGATATACGAAATTGTCAATGTCATGATCATTACTCTCTATATCTCTTCCTCTTTTTCTCCTTCGTATAATTTGTGCTCCTTCAACTTCCAGGTGCATTTTGTTGAAATGGATCCATGGGTTGTTTCTGATGTTTTAAGGCCAAATCTGCAATCTACTGGGTTTCTTGATGACTCAGTCATTCACACAATTCCAGTTGAAAATTTTATAGAACGAGCTGAGCAGCTTATTGGTATCCCATCTAAACTTCAGTTTTCTCCTTCCTTGTTTGTTTATCCTAAGAAGTACAGACACAAACATGGACAATAGGGCACGTTGTTTCTAGCAAACTAAGACACTTATAGAAACAAAGATAGAAAGATACATCTATTTTTTATGCATCTAATGTCATGTTAAAATTATTTTAATTCATGATATATTTATTTAATATATTAAAATGGAGAATTGTGTTTGAGAATATAATTGTTACTCTTGTTTAAGTGTCCAGCAAGTTTTTGATATATGCCCAAAGTCGGAAAGTGAAGTGTTCAATACTCATCAGCCACACGACACATATGTATTCTAATTAAAGCTCCCATGCTTCATAGATTTTATCTGATGTGAATGCAGAACATACAATAATTTTCCATTTTTCAAAATCCGGTTTATGTTTGACTAAACAGCAACAATAAATGGGGGGGTATAGATCTCCACAATTGAACTGCATTCAAATTTTGAAGGGGATACAGCTTGGCTTGATTTCAAAATGCTTATATACAATAATCAATAATGGATAGTTTCGTCAACTGATAGTTACCAATGGTTGACAGCTTCTATATATATCAGAGTTGTTCAAGAATAGTCTTCTTTAAGCTTTTGATACAGATGGAAAATGGAGTAGCACAATAACGAATAACCTAATCCCTTAGCATCCTATTACGCAAATAAAACCCTGATTGAGGTTTAGAATTGGTCAATCTGGCATAAAAACATTGAATAGATTCTGCATTATCATTCAAGTCTGATCTGTAACATTCATGACTCAAATAAAGAATGCACTATTGCAGAAGATACTATCGATGTACTGCTCCCAAAATGTGTAGCACCCAAAGTTTTAAGAAATTTATCTGTTGTTGTGTCATTAAACTTGCATAACTTTGTTATCAAATTAAGGAATTAGAGTAAAGATGTAAATGAAAATGCTAAATGCAAATGGTCTATACATGTCTCACCTCAGTAGGAACAAAGAAATCGGTAAAATCACACAAAATTTCCCCTTTTCTATTAGAAGAATAAACTGTAGTGGAACAATTTCGTATGATATAGTTCAATTGAATGTCTAAGCTTCTATATTATATGATTTTAAAACTGCCCGATTATTATGTCAAGAATATTGTCTTCTCTAGGTAATGATAGGCCATTTGATTACATTAGTGTTACCCCGCCCTACACGCAAGTTGATTATGGGGTACTCATGGGGCAACTATCAAAGTCAGCCTTAATTGGTGAAGATACCTTCATTGTAAGTTGTAATTTTCTTGTTCTTGCTTAAGAAGTTTTTACATATGGTTTACCTTCCTTTCTGTCAATTTGATCTTGTGATCTAGGTGGTTGAATATCCATTAAGGACTGACATGTTGGATTCGTGTGGATGCCTTATTAAGGTAATTTAATCGTGCAGGTATTTTACATTCTTAATGTTATGAATGAATGAAATAATTAATTGGACAAAATTGTGTTTCGTTTGAAATTTTTTCACTGAATATACAATATCTCTAGCCTGATGGAATTTAGTGAGAAGTTATGGGCATGGATCCTGAGCGATTATATTTTTTGTAATTGGGTCAAGTTAACTGTAGTCTGTAATGTCACCAACACCAAGAGTAATTTCTCAAGGCTCTTGAAAAATATATATGCAAGAAGGGATCAAGGGTCTGGTATCTTACGAATTTCTGCCGTTGCTTTCTTTGTATTTTTTGAAATCTGAACATGGGATCTACGTTTGGTGAAAATTATTTCGGGTTTTTCTCTTAACTTTTTCCCCGTTGAGTAACAAATATATTTGTATCTAACTGCAGATTACTGACCGATGGTTTGGTCGGACACAGTTGGCAATATATGGACCCAAGTGGGCTGAGAAGAAAAAAAAAGAAAAAATTAAAATCACTTGATGAAATTACTATAACCGCCAAGTGAAATTAGCTGGTGACTTTGTTCCGCAACAATTGCTACGAGCCTAAACTCAAGTGGTCGGAAGGCAACTTAAAGGAGCTGCATGCTTTAAAGCATTGCCATCGTTCCATTCTGTTGGTAACTAGGAACTGAAGTATCCAGATAGATGTTGATACTACTATTCTTGATTCTATTGGCATCTTGCAAGTACAGGGTTCCCAACTATTGCAGGTCAAGGCTGTGTCTGTGATCTACAGCCATCTGCGCATTGTCTAGTTCTAGAATTTATATCCAAGTTTCATGACGGTAAGGCTTAAAGAAAACAATCAGAGAATGTACCACCTAATTATGTCCTATTTCTTTTTTCTTTTTTTTTTTTTTTTGGTCCAAATCGACGAGTACTATGTACTTGAGCAGTTGCCAAGTAGATTTTCCCACAGTGAGCAAAAGTATAACCTGTAGAAGATTGTATTGGAACTCAAATATCTTTCATCGACCTTTTCGGTTGATTCCAGCATTTAAGTGCCAGTACCTGTGTAGTATTTAGAATCATGTATTTTATGGACTGTTGATCTCCAAGTTTACCCTAGTCATTCTTGTGCTGCTTTATTTATTCCAAACTTCTGTTTTACTCAACTAGTGGGAATCTCATCATATCTTATTTGATTTTACAAAAGTTCTTAGGTTTTTTTGCCTTTGCTTAGCAAGAAAAATTAGAATCCTGGATAACTTTGCTGACACATTTTTTTCTGACTCTAAAAATATTGTTGTTAATATTATTATTACCATTTGCGTGTGTG

mRNA sequence

CTCCGTTGATGGCGGTTTCTTCGTTCCCGGTTCTTTCTTCATTGAGTGCCAGTGTTAATATCTCTTCGGATGTTAGCTTGTTTTCTCTGAATCTTCCACTCGTCAATTTGTTTTACAGATCGAGCACGCATCCGTCGTCTTCTACTATCGTTTGCTCGTACAAATCTGGGAATGATACGGTGACGGAGCGGAGGAAAAAGTTGTTGGAGCAATATGGACTTGATCCTGACGAGTTTCTGTCTGAATCTTCTACTAATAGAAAAAGAGAAGTGCTAAAATCGGGAAAAGGCAAGCAGGGTTCAGCCGAGGATCCGAAACCTCAACGGCAGACCCATAAGTTACTTCAGGTGCTTGGAGGTAGTGCTCGGAGAATGAAGCTACTCTCACCAAAGGGCTTGGATGTACGACCGATGATGGAAGTTGTGAAAGGTGCAGCTTTTGATATTTTGCAGGCTGCTGGTGGCTGTCCAGCATTTTTAAGGCCTGGTCGTTGGTTAGACTTGTATAGTGGTACTGGATCAGTTGGTATTGAAGCTATGAGCCGTGGATGTTCTGAGGTGCATTTTGTTGAAATGGATCCATGGGTTGTTTCTGATGTTTTAAGGCCAAATCTGCAATCTACTGGGTTTCTTGATGACTCAGTCATTCACACAATTCCAGTTGAAAATTTTATAGAACGAGCTGAGCAGCTTATTGGTAATGATAGGCCATTTGATTACATTAGTGTTACCCCGCCCTACACGCAAGTTGATTATGGGGTACTCATGGGGCAACTATCAAAGTCAGCCTTAATTGGTGAAGATACCTTCATTGTGGTTGAATATCCATTAAGGACTGACATGTTGGATTCGTGTGGATGCCTTATTAAGATTACTGACCGATGGTTTGGTCGGACACAGTTGGCAATATATGGACCCAAGTGGGCTGAGAAGAAAAAAAAAGAAAAAATTAAAATCACTTGATGAAATTACTATAACCGCCAAGTGAAATTAGCTGGTGACTTTGTTCCGCAACAATTGCTACGAGCCTAAACTCAAGTGGTCGGAAGGCAACTTAAAGGAGCTGCATGCTTTAAAGCATTGCCATCGTTCCATTCTGTTGGTAACTAGGAACTGAAGTATCCAGATAGATGTTGATACTACTATTCTTGATTCTATTGGCATCTTGCAAGTACAGGGTTCCCAACTATTGCAGGTCAAGGCTGTGTCTGTGATCTACAGCCATCTGCGCATTGTCTAGTTCTAGAATTTATATCCAAGTTTCATGACGGTAAGGCTTAAAGAAAACAATCAGAGAATGTACCACCTAATTATGTCCTATTTCTTTTTTCTTTTTTTTTTTTTTTTGGTCCAAATCGACGAGTACTATGTACTTGAGCAGTTGCCAAGTAGATTTTCCCACAGTGAGCAAAAGTATAACCTGTAGAAGATTGTATTGGAACTCAAATATCTTTCATCGACCTTTTCGGTTGATTCCAGCATTTAAGTGCCAGTACCTGTGTAGTATTTAGAATCATGTATTTTATGGACTGTTGATCTCCAAGTTTACCCTAGTCATTCTTGTGCTGCTTTATTTATTCCAAACTTCTGTTTTACTCAACTAGTGGGAATCTCATCATATCTTATTTGATTTTACAAAAGTTCTTAGGTTTTTTTGCCTTTGCTTAGCAAGAAAAATTAGAATCCTGGATAACTTTGCTGACACATTTTTTTCTGACTCTAAAAATATTGTTGTTAATATTATTATTACCATTTGCGTGTGTG

Coding sequence (CDS)

ATGGCGGTTTCTTCGTTCCCGGTTCTTTCTTCATTGAGTGCCAGTGTTAATATCTCTTCGGATGTTAGCTTGTTTTCTCTGAATCTTCCACTCGTCAATTTGTTTTACAGATCGAGCACGCATCCGTCGTCTTCTACTATCGTTTGCTCGTACAAATCTGGGAATGATACGGTGACGGAGCGGAGGAAAAAGTTGTTGGAGCAATATGGACTTGATCCTGACGAGTTTCTGTCTGAATCTTCTACTAATAGAAAAAGAGAAGTGCTAAAATCGGGAAAAGGCAAGCAGGGTTCAGCCGAGGATCCGAAACCTCAACGGCAGACCCATAAGTTACTTCAGGTGCTTGGAGGTAGTGCTCGGAGAATGAAGCTACTCTCACCAAAGGGCTTGGATGTACGACCGATGATGGAAGTTGTGAAAGGTGCAGCTTTTGATATTTTGCAGGCTGCTGGTGGCTGTCCAGCATTTTTAAGGCCTGGTCGTTGGTTAGACTTGTATAGTGGTACTGGATCAGTTGGTATTGAAGCTATGAGCCGTGGATGTTCTGAGGTGCATTTTGTTGAAATGGATCCATGGGTTGTTTCTGATGTTTTAAGGCCAAATCTGCAATCTACTGGGTTTCTTGATGACTCAGTCATTCACACAATTCCAGTTGAAAATTTTATAGAACGAGCTGAGCAGCTTATTGGTAATGATAGGCCATTTGATTACATTAGTGTTACCCCGCCCTACACGCAAGTTGATTATGGGGTACTCATGGGGCAACTATCAAAGTCAGCCTTAATTGGTGAAGATACCTTCATTGTGGTTGAATATCCATTAAGGACTGACATGTTGGATTCGTGTGGATGCCTTATTAAGATTACTGACCGATGGTTTGGTCGGACACAGTTGGCAATATATGGACCCAAGTGGGCTGAGAAGAAAAAAAAAGAAAAAATTAAAATCACTTGA

Protein sequence

MAVSSFPVLSSLSASVNISSDVSLFSLNLPLVNLFYRSSTHPSSSTIVCSYKSGNDTVTERRKKLLEQYGLDPDEFLSESSTNRKREVLKSGKGKQGSAEDPKPQRQTHKLLQVLGGSARRMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPAFLRPGRWLDLYSGTGSVGIEAMSRGCSEVHFVEMDPWVVSDVLRPNLQSTGFLDDSVIHTIPVENFIERAEQLIGNDRPFDYISVTPPYTQVDYGVLMGQLSKSALIGEDTFIVVEYPLRTDMLDSCGCLIKITDRWFGRTQLAIYGPKWAEKKKKEKIKIT
Homology
BLAST of Lcy09g004180 vs. ExPASy Swiss-Prot
Match: O34331 (Putative rRNA methyltransferase YlbH OS=Bacillus subtilis (strain 168) OX=224308 GN=ylbH PE=3 SV=2)

HSP 1 Score: 83.6 bits (205), Expect = 4.5e-15
Identity = 51/190 (26.84%), Postives = 93/190 (48.95%), Query Frame = 0

Query: 112 LQVLGGSARRMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPAFLRPGRWLDLYSGTGS 171
           ++V+ GS +   L +  G   RP  + VK + F+++        +   GR LDL++G+G 
Sbjct: 1   MRVISGSKKGRSLKAVAGTSTRPTTDKVKESIFNMI------GPYFDGGRGLDLFAGSGG 60

Query: 172 VGIEAMSRGCSEVHFVEMDPWVVSDVLRPNLQSTGFLDDSVIHTIPVENFIERAEQLIGN 231
           +GIEA+SRG     FV+ D   +  V + NL++      + ++    E  +  A +    
Sbjct: 61  LGIEALSRGFEHCIFVDRDFKAIQTV-KSNLKTLELTKHAQVYRNDAERALHAAAK---R 120

Query: 232 DRPFDYISVTPPYTQVDYGVLMGQLSKSALIGEDTFIVVEYPLRTDMLDSCGCLIKITDR 291
           +  F  I + PPY +     L+  + +  ++ ED FIV E+    ++ ++ G L+     
Sbjct: 121 ETGFRGIFLDPPYKEQKLKALLTLIDEYQMLEEDGFIVAEHDREVELPETVGDLVMTRKE 180

Query: 292 WFGRTQLAIY 302
            +G T +AIY
Sbjct: 181 TYGLTGVAIY 180

BLAST of Lcy09g004180 vs. ExPASy Swiss-Prot
Match: Q9ZD05 (Uncharacterized methylase RP545 OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=RP545 PE=4 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 6.5e-06
Identity = 51/233 (21.89%), Postives = 96/233 (41.20%), Query Frame = 0

Query: 111 LLQVLGGSARRMKLLSPKGLDVRPMMEVVKGAAFDILQAAG--GCPAFLRPGRWLDLYSG 170
           +L+++ G  +   + + + +  RP    +K A F IL +    G   F    + LDL++G
Sbjct: 1   MLKIISGKYKNQIIPTAQNIKYRPSTGKLKEAIFSILTSGEFIGNKLFNENTQILDLFAG 60

Query: 171 TGSVGIEAMSRGCSEVHFVEMDPWVVS------------------DVLRPNLQ-STGFLD 230
           +GS+  E++SRG      +++D + +                   ++   NLQ +T +L 
Sbjct: 61  SGSLAFESLSRGAGFATLIDIDTYSLKIAAGFAKSLNIENNVHFININALNLQKTTRYLS 120

Query: 231 DSVIHTIPVENFIERAEQLIG----------------------NDRPFDYISVTPPYTQV 290
                      FI  AE  IG                      +++ FD + + PPY + 
Sbjct: 121 KQTDR----NEFITTAESYIGISKHKSTNITYKLPLKEQFCNMSNKVFDLVFIDPPYNKD 180

Query: 291 DYGVLMGQLSKSALIGEDTFIVVEYPLRTDMLDSCGCLIKITDRWFGRTQLAI 301
               +M  L K+  +   T IV+E   +TD  D    +  I  + +G+++L +
Sbjct: 181 IVPKVMKLLIKNNWLKNGTIIVIEMS-KTDDYDLDKNIEIIRAKLYGQSKLLV 228

BLAST of Lcy09g004180 vs. ExPASy Swiss-Prot
Match: P0ADY0 (Ribosomal RNA small subunit methyltransferase D OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) OX=199310 GN=rsmD PE=3 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 2.5e-05
Identity = 39/169 (23.08%), Postives = 75/169 (44.38%), Query Frame = 0

Query: 103 KPQRQTHKLLQVLGGSARRMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPAFLRPGRW 162
           KP       ++++GG  R  KL  P    +RP  + V+   F+ L         +   + 
Sbjct: 3   KPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPV------IVDAQC 62

Query: 163 LDLYSGTGSVGIEAMSRGCSEVHFVEMDPWVVSDVLRPNLQSTGFLDDSVIHTIPVENFI 222
           LD ++G+G++G+EA+SR  +    +EMD   VS  L  NL +    +  V+++  +    
Sbjct: 63  LDCFAGSGALGLEALSRYAAGATLIEMDR-AVSQQLIKNLATLKAGNARVVNSNAMSFLA 122

Query: 223 ERAEQLIGNDRPFDYISVTPPYTQVDYGVLMGQLSKSALIGEDTFIVVE 272
           ++         P + + V PP+ +      +  L  +  + ++  I VE
Sbjct: 123 QKG-------TPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVE 157

BLAST of Lcy09g004180 vs. ExPASy Swiss-Prot
Match: P0ADX9 (Ribosomal RNA small subunit methyltransferase D OS=Escherichia coli (strain K12) OX=83333 GN=rsmD PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 2.5e-05
Identity = 39/169 (23.08%), Postives = 75/169 (44.38%), Query Frame = 0

Query: 103 KPQRQTHKLLQVLGGSARRMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPAFLRPGRW 162
           KP       ++++GG  R  KL  P    +RP  + V+   F+ L         +   + 
Sbjct: 3   KPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPV------IVDAQC 62

Query: 163 LDLYSGTGSVGIEAMSRGCSEVHFVEMDPWVVSDVLRPNLQSTGFLDDSVIHTIPVENFI 222
           LD ++G+G++G+EA+SR  +    +EMD   VS  L  NL +    +  V+++  +    
Sbjct: 63  LDCFAGSGALGLEALSRYAAGATLIEMDR-AVSQQLIKNLATLKAGNARVVNSNAMSFLA 122

Query: 223 ERAEQLIGNDRPFDYISVTPPYTQVDYGVLMGQLSKSALIGEDTFIVVE 272
           ++         P + + V PP+ +      +  L  +  + ++  I VE
Sbjct: 123 QKG-------TPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVE 157

BLAST of Lcy09g004180 vs. ExPASy Swiss-Prot
Match: P57136 (Ribosomal RNA small subunit methyltransferase D OS=Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) OX=107806 GN=rsmD PE=3 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 3.2e-05
Identity = 34/160 (21.25%), Postives = 77/160 (48.12%), Query Frame = 0

Query: 112 LQVLGGSARRMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPAFLRPGRWLDLYSGTGS 171
           + ++ G+ +  K+      ++RP    ++   F+ L        +++  R LD ++G+G 
Sbjct: 12  IYIISGNLKGRKISFKNIPNLRPTTNQIRETLFEWLS------KYIKNSRCLDCFAGSGV 71

Query: 172 VGIEAMSRGCSEVHFVEMDPWVVSDVLRPNLQSTGFLDDSVIHTIPVENFIERAEQLIGN 231
           +GIEA+SR  +    +E++       L+ N++     +  +I T  + +++++       
Sbjct: 72  LGIEAISRYAAFSTLLEIEKKTFL-TLKKNIKELNIYNVEIIRTNTL-HWLKKT-----R 131

Query: 232 DRPFDYISVTPPYTQVDYGVLMGQLSKSALIGEDTFIVVE 272
           ++P+D I + PPY Q      +  L     I +++FI +E
Sbjct: 132 NKPYDIIFIDPPYHQGLVKKTINLLENKKWIKKNSFIYIE 158

BLAST of Lcy09g004180 vs. ExPASy TrEMBL
Match: A0A6J1BZW0 (uncharacterized protein LOC111006220 OS=Momordica charantia OX=3673 GN=LOC111006220 PE=4 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 6.0e-156
Identity = 282/316 (89.24%), Postives = 297/316 (93.99%), Query Frame = 0

Query: 1   MAVSSFPVLSSLSASVNISSDVSLFSLNLPLVNLFYRSSTHPSSSTIVCSYKSGNDTVTE 60
           MAVSSFPVLSSLSAS NIS+DVSL+SLNLPLVN+  R S H SSSTIVCSYKSG++ VT+
Sbjct: 1   MAVSSFPVLSSLSASANISADVSLYSLNLPLVNICSRLSAHQSSSTIVCSYKSGSEAVTD 60

Query: 61  RRKKLLEQYGLDPDEFLSESSTNRKREVLKSGKGKQGSAEDPKPQRQTHKLLQVLGGSAR 120
           RRKKLLEQYGLDPDEFLSE STNR+REV KSGKGKQ SA DPKPQR+THKLLQVLGGSAR
Sbjct: 61  RRKKLLEQYGLDPDEFLSEPSTNRRREVPKSGKGKQISAGDPKPQRETHKLLQVLGGSAR 120

Query: 121 RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPAFLRPGRWLDLYSGTGSVGIEAMSRG 180
           RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPA LRPGRWLDLYSGTGSVGIEAMSRG
Sbjct: 121 RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPASLRPGRWLDLYSGTGSVGIEAMSRG 180

Query: 181 CSEVHFVEMDPWVVSDVLRPNLQSTGFLDDSVIHTIPVENFIERAEQLIGNDRPFDYISV 240
           CSEVHFVEMDPWVVSDVLRPNL+STGFLD SVIHTIPVENFIERA+Q IGND PFDYISV
Sbjct: 181 CSEVHFVEMDPWVVSDVLRPNLESTGFLDVSVIHTIPVENFIERAKQFIGNDGPFDYISV 240

Query: 241 TPPYTQVDYGVLMGQLSKSALIGEDTFIVVEYPLRTDMLDSCGCLIKITDRWFGRTQLAI 300
           TPPYTQVDYGVLMGQLS SAL+GEDTFIVVEYPLRT+MLDSCGCLIKITDR FGRT LAI
Sbjct: 241 TPPYTQVDYGVLMGQLSTSALVGEDTFIVVEYPLRTEMLDSCGCLIKITDRRFGRTHLAI 300

Query: 301 YGPKWAEKKKKEKIKI 317
           YGPKWAEKKK++K K+
Sbjct: 301 YGPKWAEKKKRKKEKL 316

BLAST of Lcy09g004180 vs. ExPASy TrEMBL
Match: A0A5D3BK68 (Putative rRNA methyltransferase YlbH OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1582G00020 PE=4 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 1.3e-150
Identity = 275/313 (87.86%), Postives = 290/313 (92.65%), Query Frame = 0

Query: 1   MAVSSFPVLSSLSASVNISSDVSLFSLNLPLVNLFYRSSTHPSSSTIVCSYKSGNDTVTE 60
           MAVSSFPVLSSLSAS NI SDVS FSL+ PL+N+  +SS+HPSSS IVCSYKSGN TV E
Sbjct: 1   MAVSSFPVLSSLSASANI-SDVSSFSLSSPLINICSKSSSHPSSSIIVCSYKSGNHTVAE 60

Query: 61  RRKKLLEQYGLDPDEFLSESSTNRKREVLKSGKGKQGSAEDPKPQRQTHKLLQVLGGSAR 120
           R+KKLLEQYGLDP+EFLSE STNR+RE+ K+GKGKQ S EDPKPQRQTHKLLQVLGGSAR
Sbjct: 61  RKKKLLEQYGLDPNEFLSEPSTNRRREIRKTGKGKQISLEDPKPQRQTHKLLQVLGGSAR 120

Query: 121 RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPAFLRPGRWLDLYSGTGSVGIEAMSRG 180
           RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPA LRPGRWLDLYSGTGSVGIEAMSRG
Sbjct: 121 RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPASLRPGRWLDLYSGTGSVGIEAMSRG 180

Query: 181 CSEVHFVEMDPWVVSDVLRPNLQSTGFLDDSVIHTIPVENFIERAEQLIGNDRPFDYISV 240
           CSEVHFVEMDPWVVSDVLRPNL+STGF D SVIHTI VENFIERA+QLIG + PFDYISV
Sbjct: 181 CSEVHFVEMDPWVVSDVLRPNLESTGFDDVSVIHTIRVENFIERADQLIGTETPFDYISV 240

Query: 241 TPPYTQVDYGVLMGQLSKSALIGEDTFIVVEYPLRTDMLDSCGCLIKITDRWFGRTQLAI 300
           TPPYTQVDYGVLMGQLSKSAL+GEDTFIVVEYPL+TDMLDSCGCL KITDR FGRT LAI
Sbjct: 241 TPPYTQVDYGVLMGQLSKSALVGEDTFIVVEYPLKTDMLDSCGCLTKITDRRFGRTHLAI 300

Query: 301 YGPKWAEKKKKEK 314
           YGPKWAEKKKK K
Sbjct: 301 YGPKWAEKKKKGK 312

BLAST of Lcy09g004180 vs. ExPASy TrEMBL
Match: A0A1S3C8K7 (putative rRNA methyltransferase YlbH OS=Cucumis melo OX=3656 GN=LOC103498032 PE=4 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 1.3e-150
Identity = 275/313 (87.86%), Postives = 290/313 (92.65%), Query Frame = 0

Query: 1   MAVSSFPVLSSLSASVNISSDVSLFSLNLPLVNLFYRSSTHPSSSTIVCSYKSGNDTVTE 60
           MAVSSFPVLSSLSAS NI SDVS FSL+ PL+N+  +SS+HPSSS IVCSYKSGN TV E
Sbjct: 1   MAVSSFPVLSSLSASANI-SDVSSFSLSSPLINICSKSSSHPSSSIIVCSYKSGNHTVAE 60

Query: 61  RRKKLLEQYGLDPDEFLSESSTNRKREVLKSGKGKQGSAEDPKPQRQTHKLLQVLGGSAR 120
           R+KKLLEQYGLDP+EFLSE STNR+RE+ K+GKGKQ S EDPKPQRQTHKLLQVLGGSAR
Sbjct: 61  RKKKLLEQYGLDPNEFLSEPSTNRRREIRKTGKGKQISLEDPKPQRQTHKLLQVLGGSAR 120

Query: 121 RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPAFLRPGRWLDLYSGTGSVGIEAMSRG 180
           RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPA LRPGRWLDLYSGTGSVGIEAMSRG
Sbjct: 121 RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPASLRPGRWLDLYSGTGSVGIEAMSRG 180

Query: 181 CSEVHFVEMDPWVVSDVLRPNLQSTGFLDDSVIHTIPVENFIERAEQLIGNDRPFDYISV 240
           CSEVHFVEMDPWVVSDVLRPNL+STGF D SVIHTI VENFIERA+QLIG + PFDYISV
Sbjct: 181 CSEVHFVEMDPWVVSDVLRPNLESTGFDDVSVIHTIRVENFIERADQLIGTETPFDYISV 240

Query: 241 TPPYTQVDYGVLMGQLSKSALIGEDTFIVVEYPLRTDMLDSCGCLIKITDRWFGRTQLAI 300
           TPPYTQVDYGVLMGQLSKSAL+GEDTFIVVEYPL+TDMLDSCGCL KITDR FGRT LAI
Sbjct: 241 TPPYTQVDYGVLMGQLSKSALVGEDTFIVVEYPLKTDMLDSCGCLTKITDRRFGRTHLAI 300

Query: 301 YGPKWAEKKKKEK 314
           YGPKWAEKKKK K
Sbjct: 301 YGPKWAEKKKKGK 312

BLAST of Lcy09g004180 vs. ExPASy TrEMBL
Match: A0A0A0KX89 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G633240 PE=4 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 2.2e-150
Identity = 275/313 (87.86%), Postives = 288/313 (92.01%), Query Frame = 0

Query: 1   MAVSSFPVLSSLSASVNISSDVSLFSLNLPLVNLFYRSSTHPSSSTIVCSYKSGNDTVTE 60
           MAVSS PVLSSLSAS NI SDV+ FSLN PL N+  + S HPSSS IVCSYKSGN TVTE
Sbjct: 1   MAVSSLPVLSSLSASANI-SDVTSFSLNSPLSNICSKPSAHPSSSIIVCSYKSGNHTVTE 60

Query: 61  RRKKLLEQYGLDPDEFLSESSTNRKREVLKSGKGKQGSAEDPKPQRQTHKLLQVLGGSAR 120
           R+KKLLEQYGLDP+EFLSE STNR+RE+ K+GKGKQ   EDPKPQRQTHKLLQVLGGSAR
Sbjct: 61  RKKKLLEQYGLDPNEFLSEPSTNRRREMRKTGKGKQIFPEDPKPQRQTHKLLQVLGGSAR 120

Query: 121 RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPAFLRPGRWLDLYSGTGSVGIEAMSRG 180
           RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPA LRPGRWLDLYSGTGSVGIEAMSRG
Sbjct: 121 RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPASLRPGRWLDLYSGTGSVGIEAMSRG 180

Query: 181 CSEVHFVEMDPWVVSDVLRPNLQSTGFLDDSVIHTIPVENFIERAEQLIGNDRPFDYISV 240
           CSEVHFVEMDPWVVSDVLRPNL+STGF D SVIHTI VENFIERA+QLIG +RPFDYISV
Sbjct: 181 CSEVHFVEMDPWVVSDVLRPNLESTGFFDVSVIHTIRVENFIERADQLIGTERPFDYISV 240

Query: 241 TPPYTQVDYGVLMGQLSKSALIGEDTFIVVEYPLRTDMLDSCGCLIKITDRWFGRTQLAI 300
           TPPYTQVDYGVLMGQLSKSAL+GEDTFIVVEYPLRTDML+SCGCL KITDR FGRT LAI
Sbjct: 241 TPPYTQVDYGVLMGQLSKSALVGEDTFIVVEYPLRTDMLESCGCLTKITDRRFGRTHLAI 300

Query: 301 YGPKWAEKKKKEK 314
           YGPKWAEKKKKEK
Sbjct: 301 YGPKWAEKKKKEK 312

BLAST of Lcy09g004180 vs. ExPASy TrEMBL
Match: A0A6J1K2L1 (uncharacterized protein LOC111489523 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489523 PE=4 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 1.3e-145
Identity = 270/316 (85.44%), Postives = 287/316 (90.82%), Query Frame = 0

Query: 1   MAVSSFPVLSSLSASVNIS---SDVSLFSLNLPLVNLFYRSSTHPSSSTIVCSYKSGNDT 60
           MAVSSFPVLSSL A  NIS    D SLFSLN+PLVN+  R STHPSSSTIVCSYKS N +
Sbjct: 300 MAVSSFPVLSSLGARSNISVLDRDCSLFSLNIPLVNICSRPSTHPSSSTIVCSYKSEN-S 359

Query: 61  VTERRKKLLEQYGLDPDEFLSESSTNRKREVLKSGKGKQGSAEDPKPQRQTHKLLQVLGG 120
           V+ERRKKLLEQYGLDPDEFLSE S NR RE+ KSGK KQ S +DPK QR+THKLL+VLGG
Sbjct: 360 VSERRKKLLEQYGLDPDEFLSEPSANRAREMRKSGKDKQISGQDPKFQRKTHKLLKVLGG 419

Query: 121 SARRMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPAFLRPGRWLDLYSGTGSVGIEAM 180
           SARRM+LLSP GLDVRPMMEVVKGAAFDILQAAGGCPA LRPGRWLDLYSGTGSVGIEAM
Sbjct: 420 SARRMRLLSPIGLDVRPMMEVVKGAAFDILQAAGGCPASLRPGRWLDLYSGTGSVGIEAM 479

Query: 181 SRGCSEVHFVEMDPWVVSDVLRPNLQSTGFLDDSVIHTIPVENFIERAEQLIGNDRPFDY 240
           SRGCSEVHFVEMDPWVVSDVLRPNL+STGF DDSVIHTIPVENFIERAEQLIGNDRPFDY
Sbjct: 480 SRGCSEVHFVEMDPWVVSDVLRPNLESTGFFDDSVIHTIPVENFIERAEQLIGNDRPFDY 539

Query: 241 ISVTPPYTQVDYGVLMGQLSKSALIGEDTFIVVEYPLRTDMLDSCGCLIKITDRWFGRTQ 300
           ISVTPPY QVDYGV+M QLSKSAL+GEDTFIVVEYPL+TDMLDSCGCLI+IT R FGRT 
Sbjct: 540 ISVTPPYIQVDYGVVMEQLSKSALVGEDTFIVVEYPLKTDMLDSCGCLIQITKRRFGRTV 599

Query: 301 LAIYGPKWAEKKKKEK 314
           LAIYGPKWAEK+K++K
Sbjct: 600 LAIYGPKWAEKEKRKK 614

BLAST of Lcy09g004180 vs. NCBI nr
Match: XP_038890433.1 (putative rRNA methyltransferase YlbH [Benincasa hispida])

HSP 1 Score: 566.2 bits (1458), Expect = 1.7e-157
Identity = 283/313 (90.42%), Postives = 298/313 (95.21%), Query Frame = 0

Query: 1   MAVSSFPVLSSLSASVNISSDVSLFSLNLPLVNLFYRSSTHPSSSTIVCSYKSGNDTVTE 60
           MAVSSFPVLSSLSASV+IS DVS FSLN+PLVN+  + +THPSSS IVCSYKSGN+ VTE
Sbjct: 1   MAVSSFPVLSSLSASVSISPDVSSFSLNIPLVNICSKQTTHPSSSIIVCSYKSGNNAVTE 60

Query: 61  RRKKLLEQYGLDPDEFLSESSTNRKREVLKSGKGKQGSAEDPKPQRQTHKLLQVLGGSAR 120
           RRK+LLEQYGLDP+EFLSE STNR+RE+ K+G+GKQ SAEDPKPQRQTHKLLQVLGGSAR
Sbjct: 61  RRKRLLEQYGLDPNEFLSEPSTNRRREMRKTGEGKQISAEDPKPQRQTHKLLQVLGGSAR 120

Query: 121 RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPAFLRPGRWLDLYSGTGSVGIEAMSRG 180
           RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPA LRPGRWLDLYSGTGSVGIEAMSRG
Sbjct: 121 RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPASLRPGRWLDLYSGTGSVGIEAMSRG 180

Query: 181 CSEVHFVEMDPWVVSDVLRPNLQSTGFLDDSVIHTIPVENFIERAEQLIGNDRPFDYISV 240
           CSEVHFVEMDPWVVSDVLRPNL+STGF DDSVIHTI VENFI+RAEQLIGNDRPFDYISV
Sbjct: 181 CSEVHFVEMDPWVVSDVLRPNLESTGFFDDSVIHTIRVENFIDRAEQLIGNDRPFDYISV 240

Query: 241 TPPYTQVDYGVLMGQLSKSALIGEDTFIVVEYPLRTDMLDSCGCLIKITDRWFGRTQLAI 300
           TPPYTQVDYGVLMGQLSKSAL+GEDTFIVVEYPLRTDMLDSCGCLIKITDR FGRT LAI
Sbjct: 241 TPPYTQVDYGVLMGQLSKSALVGEDTFIVVEYPLRTDMLDSCGCLIKITDRRFGRTHLAI 300

Query: 301 YGPKWAEKKKKEK 314
           YGPKWAEKKKKEK
Sbjct: 301 YGPKWAEKKKKEK 313

BLAST of Lcy09g004180 vs. NCBI nr
Match: XP_022133703.1 (uncharacterized protein LOC111006220 [Momordica charantia])

HSP 1 Score: 560.1 bits (1442), Expect = 1.2e-155
Identity = 282/316 (89.24%), Postives = 297/316 (93.99%), Query Frame = 0

Query: 1   MAVSSFPVLSSLSASVNISSDVSLFSLNLPLVNLFYRSSTHPSSSTIVCSYKSGNDTVTE 60
           MAVSSFPVLSSLSAS NIS+DVSL+SLNLPLVN+  R S H SSSTIVCSYKSG++ VT+
Sbjct: 1   MAVSSFPVLSSLSASANISADVSLYSLNLPLVNICSRLSAHQSSSTIVCSYKSGSEAVTD 60

Query: 61  RRKKLLEQYGLDPDEFLSESSTNRKREVLKSGKGKQGSAEDPKPQRQTHKLLQVLGGSAR 120
           RRKKLLEQYGLDPDEFLSE STNR+REV KSGKGKQ SA DPKPQR+THKLLQVLGGSAR
Sbjct: 61  RRKKLLEQYGLDPDEFLSEPSTNRRREVPKSGKGKQISAGDPKPQRETHKLLQVLGGSAR 120

Query: 121 RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPAFLRPGRWLDLYSGTGSVGIEAMSRG 180
           RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPA LRPGRWLDLYSGTGSVGIEAMSRG
Sbjct: 121 RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPASLRPGRWLDLYSGTGSVGIEAMSRG 180

Query: 181 CSEVHFVEMDPWVVSDVLRPNLQSTGFLDDSVIHTIPVENFIERAEQLIGNDRPFDYISV 240
           CSEVHFVEMDPWVVSDVLRPNL+STGFLD SVIHTIPVENFIERA+Q IGND PFDYISV
Sbjct: 181 CSEVHFVEMDPWVVSDVLRPNLESTGFLDVSVIHTIPVENFIERAKQFIGNDGPFDYISV 240

Query: 241 TPPYTQVDYGVLMGQLSKSALIGEDTFIVVEYPLRTDMLDSCGCLIKITDRWFGRTQLAI 300
           TPPYTQVDYGVLMGQLS SAL+GEDTFIVVEYPLRT+MLDSCGCLIKITDR FGRT LAI
Sbjct: 241 TPPYTQVDYGVLMGQLSTSALVGEDTFIVVEYPLRTEMLDSCGCLIKITDRRFGRTHLAI 300

Query: 301 YGPKWAEKKKKEKIKI 317
           YGPKWAEKKK++K K+
Sbjct: 301 YGPKWAEKKKRKKEKL 316

BLAST of Lcy09g004180 vs. NCBI nr
Match: XP_008458705.1 (PREDICTED: putative rRNA methyltransferase YlbH [Cucumis melo] >KAA0037420.1 putative rRNA methyltransferase YlbH [Cucumis melo var. makuwa] >TYK00211.1 putative rRNA methyltransferase YlbH [Cucumis melo var. makuwa])

HSP 1 Score: 542.3 bits (1396), Expect = 2.7e-150
Identity = 275/313 (87.86%), Postives = 290/313 (92.65%), Query Frame = 0

Query: 1   MAVSSFPVLSSLSASVNISSDVSLFSLNLPLVNLFYRSSTHPSSSTIVCSYKSGNDTVTE 60
           MAVSSFPVLSSLSAS NI SDVS FSL+ PL+N+  +SS+HPSSS IVCSYKSGN TV E
Sbjct: 1   MAVSSFPVLSSLSASANI-SDVSSFSLSSPLINICSKSSSHPSSSIIVCSYKSGNHTVAE 60

Query: 61  RRKKLLEQYGLDPDEFLSESSTNRKREVLKSGKGKQGSAEDPKPQRQTHKLLQVLGGSAR 120
           R+KKLLEQYGLDP+EFLSE STNR+RE+ K+GKGKQ S EDPKPQRQTHKLLQVLGGSAR
Sbjct: 61  RKKKLLEQYGLDPNEFLSEPSTNRRREIRKTGKGKQISLEDPKPQRQTHKLLQVLGGSAR 120

Query: 121 RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPAFLRPGRWLDLYSGTGSVGIEAMSRG 180
           RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPA LRPGRWLDLYSGTGSVGIEAMSRG
Sbjct: 121 RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPASLRPGRWLDLYSGTGSVGIEAMSRG 180

Query: 181 CSEVHFVEMDPWVVSDVLRPNLQSTGFLDDSVIHTIPVENFIERAEQLIGNDRPFDYISV 240
           CSEVHFVEMDPWVVSDVLRPNL+STGF D SVIHTI VENFIERA+QLIG + PFDYISV
Sbjct: 181 CSEVHFVEMDPWVVSDVLRPNLESTGFDDVSVIHTIRVENFIERADQLIGTETPFDYISV 240

Query: 241 TPPYTQVDYGVLMGQLSKSALIGEDTFIVVEYPLRTDMLDSCGCLIKITDRWFGRTQLAI 300
           TPPYTQVDYGVLMGQLSKSAL+GEDTFIVVEYPL+TDMLDSCGCL KITDR FGRT LAI
Sbjct: 241 TPPYTQVDYGVLMGQLSKSALVGEDTFIVVEYPLKTDMLDSCGCLTKITDRRFGRTHLAI 300

Query: 301 YGPKWAEKKKKEK 314
           YGPKWAEKKKK K
Sbjct: 301 YGPKWAEKKKKGK 312

BLAST of Lcy09g004180 vs. NCBI nr
Match: XP_004148680.1 (uncharacterized protein LOC101209883 [Cucumis sativus] >XP_031741881.1 uncharacterized protein LOC101209883 [Cucumis sativus] >KGN52426.1 hypothetical protein Csa_007850 [Cucumis sativus])

HSP 1 Score: 541.6 bits (1394), Expect = 4.6e-150
Identity = 275/313 (87.86%), Postives = 288/313 (92.01%), Query Frame = 0

Query: 1   MAVSSFPVLSSLSASVNISSDVSLFSLNLPLVNLFYRSSTHPSSSTIVCSYKSGNDTVTE 60
           MAVSS PVLSSLSAS NI SDV+ FSLN PL N+  + S HPSSS IVCSYKSGN TVTE
Sbjct: 1   MAVSSLPVLSSLSASANI-SDVTSFSLNSPLSNICSKPSAHPSSSIIVCSYKSGNHTVTE 60

Query: 61  RRKKLLEQYGLDPDEFLSESSTNRKREVLKSGKGKQGSAEDPKPQRQTHKLLQVLGGSAR 120
           R+KKLLEQYGLDP+EFLSE STNR+RE+ K+GKGKQ   EDPKPQRQTHKLLQVLGGSAR
Sbjct: 61  RKKKLLEQYGLDPNEFLSEPSTNRRREMRKTGKGKQIFPEDPKPQRQTHKLLQVLGGSAR 120

Query: 121 RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPAFLRPGRWLDLYSGTGSVGIEAMSRG 180
           RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPA LRPGRWLDLYSGTGSVGIEAMSRG
Sbjct: 121 RMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPASLRPGRWLDLYSGTGSVGIEAMSRG 180

Query: 181 CSEVHFVEMDPWVVSDVLRPNLQSTGFLDDSVIHTIPVENFIERAEQLIGNDRPFDYISV 240
           CSEVHFVEMDPWVVSDVLRPNL+STGF D SVIHTI VENFIERA+QLIG +RPFDYISV
Sbjct: 181 CSEVHFVEMDPWVVSDVLRPNLESTGFFDVSVIHTIRVENFIERADQLIGTERPFDYISV 240

Query: 241 TPPYTQVDYGVLMGQLSKSALIGEDTFIVVEYPLRTDMLDSCGCLIKITDRWFGRTQLAI 300
           TPPYTQVDYGVLMGQLSKSAL+GEDTFIVVEYPLRTDML+SCGCL KITDR FGRT LAI
Sbjct: 241 TPPYTQVDYGVLMGQLSKSALVGEDTFIVVEYPLRTDMLESCGCLTKITDRRFGRTHLAI 300

Query: 301 YGPKWAEKKKKEK 314
           YGPKWAEKKKKEK
Sbjct: 301 YGPKWAEKKKKEK 312

BLAST of Lcy09g004180 vs. NCBI nr
Match: XP_022993553.1 (uncharacterized protein LOC111489523 isoform X1 [Cucurbita maxima] >XP_022993554.1 uncharacterized protein LOC111489523 isoform X1 [Cucurbita maxima])

HSP 1 Score: 525.8 bits (1353), Expect = 2.6e-145
Identity = 270/316 (85.44%), Postives = 287/316 (90.82%), Query Frame = 0

Query: 1   MAVSSFPVLSSLSASVNIS---SDVSLFSLNLPLVNLFYRSSTHPSSSTIVCSYKSGNDT 60
           MAVSSFPVLSSL A  NIS    D SLFSLN+PLVN+  R STHPSSSTIVCSYKS N +
Sbjct: 300 MAVSSFPVLSSLGARSNISVLDRDCSLFSLNIPLVNICSRPSTHPSSSTIVCSYKSEN-S 359

Query: 61  VTERRKKLLEQYGLDPDEFLSESSTNRKREVLKSGKGKQGSAEDPKPQRQTHKLLQVLGG 120
           V+ERRKKLLEQYGLDPDEFLSE S NR RE+ KSGK KQ S +DPK QR+THKLL+VLGG
Sbjct: 360 VSERRKKLLEQYGLDPDEFLSEPSANRAREMRKSGKDKQISGQDPKFQRKTHKLLKVLGG 419

Query: 121 SARRMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPAFLRPGRWLDLYSGTGSVGIEAM 180
           SARRM+LLSP GLDVRPMMEVVKGAAFDILQAAGGCPA LRPGRWLDLYSGTGSVGIEAM
Sbjct: 420 SARRMRLLSPIGLDVRPMMEVVKGAAFDILQAAGGCPASLRPGRWLDLYSGTGSVGIEAM 479

Query: 181 SRGCSEVHFVEMDPWVVSDVLRPNLQSTGFLDDSVIHTIPVENFIERAEQLIGNDRPFDY 240
           SRGCSEVHFVEMDPWVVSDVLRPNL+STGF DDSVIHTIPVENFIERAEQLIGNDRPFDY
Sbjct: 480 SRGCSEVHFVEMDPWVVSDVLRPNLESTGFFDDSVIHTIPVENFIERAEQLIGNDRPFDY 539

Query: 241 ISVTPPYTQVDYGVLMGQLSKSALIGEDTFIVVEYPLRTDMLDSCGCLIKITDRWFGRTQ 300
           ISVTPPY QVDYGV+M QLSKSAL+GEDTFIVVEYPL+TDMLDSCGCLI+IT R FGRT 
Sbjct: 540 ISVTPPYIQVDYGVVMEQLSKSALVGEDTFIVVEYPLKTDMLDSCGCLIQITKRRFGRTV 599

Query: 301 LAIYGPKWAEKKKKEK 314
           LAIYGPKWAEK+K++K
Sbjct: 600 LAIYGPKWAEKEKRKK 614

BLAST of Lcy09g004180 vs. TAIR 10
Match: AT3G28460.1 (methyltransferases )

HSP 1 Score: 360.9 bits (925), Expect = 1.0e-99
Identity = 192/312 (61.54%), Postives = 236/312 (75.64%), Query Frame = 0

Query: 3   VSSFPVLSSLSASVNISSDVSLFSLNLPLVNLFYRSSTHPSSSTIVCSYK-SGNDTVTER 62
           +SSF   S L  +++++ ++ +   + P ++LF  S+     + +V S K SG    +E 
Sbjct: 5   LSSF---SPLGINIDMNKNLGVSYSSFPQIHLFKGSTPLRVRTFVVSSRKNSGTGLASED 64

Query: 63  RKKLLEQYGLDP-DEFLSESSTNRKREVLKSGKGKQGSAEDPKPQ-RQTHKLLQVLGGSA 122
           +K LLE+YG D  D+F S+S   R++E   SG+  Q   E    Q R TH+LLQVL G+A
Sbjct: 65  KKLLLERYGYDANDDFGSQSKKARRKEEKMSGRNSQQVEEVVVVQPRTTHRLLQVLAGTA 124

Query: 123 RRMKLLSPKGLDVRPMMEVVKGAAFDILQAAGGCPAFLRPGRWLDLYSGTGSVGIEAMSR 182
           +R KLLS KG+DVRPMMEVVKGAAF ILQAAGGCP  LRPGRWLDLYSGTGSVGIEA+SR
Sbjct: 125 KRKKLLSLKGMDVRPMMEVVKGAAFGILQAAGGCPTSLRPGRWLDLYSGTGSVGIEAISR 184

Query: 183 GCSEVHFVEMDPWVVSDVLRPNLQSTGFLDDSVIHTIPVENFIERAEQLIGNDRPFDYIS 242
           GCSE HFVEMDPWVVS+VL+PNL+ TGF+D SVIHT  VENF+ERA++L+G D  FDYIS
Sbjct: 185 GCSEAHFVEMDPWVVSNVLQPNLEHTGFVDASVIHTARVENFLERADKLVGKDGVFDYIS 244

Query: 243 VTPPYTQVDYGVLMGQLSKSALIGEDTFIVVEYPLRTDMLDSCGCLIKITDRWFGRTQLA 302
           VTPPY +VDY VLM Q++KS  IGE+TFI+VEYP RT MLDSCGCL K+TDR FGRT LA
Sbjct: 245 VTPPYMEVDYEVLMDQIAKSPAIGENTFILVEYPSRTTMLDSCGCLEKMTDRRFGRTHLA 304

Query: 303 IYGPKWAEKKKK 312
           IYGPKWA+K +K
Sbjct: 305 IYGPKWAQKPRK 313

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O343314.5e-1526.84Putative rRNA methyltransferase YlbH OS=Bacillus subtilis (strain 168) OX=224308... [more]
Q9ZD056.5e-0621.89Uncharacterized methylase RP545 OS=Rickettsia prowazekii (strain Madrid E) OX=27... [more]
P0ADY02.5e-0523.08Ribosomal RNA small subunit methyltransferase D OS=Escherichia coli O6:H1 (strai... [more]
P0ADX92.5e-0523.08Ribosomal RNA small subunit methyltransferase D OS=Escherichia coli (strain K12)... [more]
P571363.2e-0521.25Ribosomal RNA small subunit methyltransferase D OS=Buchnera aphidicola subsp. Ac... [more]
Match NameE-valueIdentityDescription
A0A6J1BZW06.0e-15689.24uncharacterized protein LOC111006220 OS=Momordica charantia OX=3673 GN=LOC111006... [more]
A0A5D3BK681.3e-15087.86Putative rRNA methyltransferase YlbH OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3C8K71.3e-15087.86putative rRNA methyltransferase YlbH OS=Cucumis melo OX=3656 GN=LOC103498032 PE=... [more]
A0A0A0KX892.2e-15087.86Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G633240 PE=4 SV=1[more]
A0A6J1K2L11.3e-14585.44uncharacterized protein LOC111489523 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
XP_038890433.11.7e-15790.42putative rRNA methyltransferase YlbH [Benincasa hispida][more]
XP_022133703.11.2e-15589.24uncharacterized protein LOC111006220 [Momordica charantia][more]
XP_008458705.12.7e-15087.86PREDICTED: putative rRNA methyltransferase YlbH [Cucumis melo] >KAA0037420.1 put... [more]
XP_004148680.14.6e-15087.86uncharacterized protein LOC101209883 [Cucumis sativus] >XP_031741881.1 uncharact... [more]
XP_022993553.12.6e-14585.44uncharacterized protein LOC111489523 isoform X1 [Cucurbita maxima] >XP_022993554... [more]
Match NameE-valueIdentityDescription
AT3G28460.11.0e-9961.54methyltransferases [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF03602Cons_hypoth95coord: 112..301
e-value: 4.6E-44
score: 150.2
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 100..313
e-value: 2.1E-39
score: 136.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 78..107
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 76..108
NoneNo IPR availablePANTHERPTHR43542METHYLTRANSFERASEcoord: 44..312
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 112..301

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g004180.1Lcy09g004180.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0008168 methyltransferase activity