Lcy09g003720 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy09g003720
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Calcium-binding EF-hand family protein, putative
Location	Chr09: 3269834 .. 3271208 (-)
RNA-Seq Expression	Lcy09g003720
Synteny	Lcy09g003720

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGGGTCTTTGTTTTGATGTCGTAAAAAGGATTATATAATAATACAATTAGCCACCCATTTAGTAGGAAGAAGAAGAGCATGCTACATAATATTATTTTTAAAAATCAAATTAATTGACCATTTGTCCAACTTAATCAGTAATTAATTACCAATTCCAAGAACCATAATTAACAAATAATACATTTTCCTTTTCTCTCTACTTTCTCAGTCAAATAATCCCAATAAGAAAACCGCAGTTTCCGGGAAAGTCGTTTTTCCCACCATCCAAACAGAAAATTCCTCAATTACGCCAACCGACCCTTCGCCTTCAAACGAACCTTCCCATTCAACAACTCCCAAATTTATTTATTTAAACATTTCTTTGATTTCATTTCCTTTGACTTCACAACCAAAACCAAAATCCCCAGTTTCCTTCGACTTTCAACCCAATACGCGCAATCCGGGGCTTCAAATACCCCCTGTTTGAAGCCCCCATTCAACATTCGATCGATCAACCTGCAACCAACATTCAACATACATACATACTCCACTCCAGAATCCTTCATCAGTTTTCTTTTCCATCCCAATGGATTTCAGTCTCTACAACGTTTCCATGGAGGACAAGACAATCGGAATCGACCGCGCCGAAAATCCCTCTTTCCGCTTCCTCATCATGATCGATTGCTTCCGCTTCCAGAAACTCGCCTCCTGGATCTACGGCTTCGCCTCCAGAAACTACTCGCCTCTCCCCGCTTCGGAGACCCCGAGATCGGAAGACGCCGGCGGGAGGCTGAGCAGAGAGGAGATGAAGTCGGTGATGGGGAAATTAGGGCTCGTCTGCGGCGATGAATCGGAGGAATCGCTTCCTCTGTTTGGGTCGGTTGAAGAGGTGTGGAAGCTGTTCGAGGAAAGGGCGGCGTGTGCGGCGGAGGTGAAGGAGGCCTTCGATGTGTTCGATGTGAACGGCGATGGGTTCATCGATGTTGAAGAGTTGCAGAGAGTGCTGTGCGTGTTGGGGTTGAAGGAAGGGGAAGGGATTGGGAATTGCAGGAGGATGATCGAGAAGTTCGATGAGAATAAAGATGGGAGAATTGATTTTGAGGAGTTTGTGAAGTTGATGGAATCTGTGGTTTGAGGGATTTTTATGAATTCGATTCATTCAATTGTTTTATGTAGAAATTGAAATTTGTGGTCGAAATTGAAGCCGTTTGCCCCTTCGATCTTGTTTTGTAAAAGAAGCAAAGATCGAGATAGTTTCTTGATTTTTGGGGGGTAAATGGAATTGAAATTTTGGCCCTTCCTATTTAATTTAACTATGTAATTTTTATATAAATACTACACATATATTTTTAATTTATGTTCATGTTTGCACCAAAGCTTAATTTTATCAAAAAA

mRNA sequence

TTGGGTCTTTGTTTTGATGTCGTAAAAAGGATTATATAATAATACAATTAGCCACCCATTTAGTAGGAAGAAGAAGAGCATGCTACATAATATTATTTTTAAAAATCAAATTAATTGACCATTTGTCCAACTTAATCAGTAATTAATTACCAATTCCAAGAACCATAATTAACAAATAATACATTTTCCTTTTCTCTCTACTTTCTCAGTCAAATAATCCCAATAAGAAAACCGCAGTTTCCGGGAAAGTCGTTTTTCCCACCATCCAAACAGAAAATTCCTCAATTACGCCAACCGACCCTTCGCCTTCAAACGAACCTTCCCATTCAACAACTCCCAAATTTATTTATTTAAACATTTCTTTGATTTCATTTCCTTTGACTTCACAACCAAAACCAAAATCCCCAGTTTCCTTCGACTTTCAACCCAATACGCGCAATCCGGGGCTTCAAATACCCCCTGTTTGAAGCCCCCATTCAACATTCGATCGATCAACCTGCAACCAACATTCAACATACATACATACTCCACTCCAGAATCCTTCATCAGTTTTCTTTTCCATCCCAATGGATTTCAGTCTCTACAACGTTTCCATGGAGGACAAGACAATCGGAATCGACCGCGCCGAAAATCCCTCTTTCCGCTTCCTCATCATGATCGATTGCTTCCGCTTCCAGAAACTCGCCTCCTGGATCTACGGCTTCGCCTCCAGAAACTACTCGCCTCTCCCCGCTTCGGAGACCCCGAGATCGGAAGACGCCGGCGGGAGGCTGAGCAGAGAGGAGATGAAGTCGGTGATGGGGAAATTAGGGCTCGTCTGCGGCGATGAATCGGAGGAATCGCTTCCTCTGTTTGGGTCGGTTGAAGAGGTGTGGAAGCTGTTCGAGGAAAGGGCGGCGTGTGCGGCGGAGGTGAAGGAGGCCTTCGATGTGTTCGATGTGAACGGCGATGGGTTCATCGATGTTGAAGAGTTGCAGAGAGTGCTGTGCGTGTTGGGGTTGAAGGAAGGGGAAGGGATTGGGAATTGCAGGAGGATGATCGAGAAGTTCGATGAGAATAAAGATGGGAGAATTGATTTTGAGGAGTTTGTGAAGTTGATGGAATCTGTGGTTTGAGGGATTTTTATGAATTCGATTCATTCAATTGTTTTATGTAGAAATTGAAATTTGTGGTCGAAATTGAAGCCGTTTGCCCCTTCGATCTTGTTTTGTAAAAGAAGCAAAGATCGAGATAGTTTCTTGATTTTTGGGGGGTAAATGGAATTGAAATTTTGGCCCTTCCTATTTAATTTAACTATGTAATTTTTATATAAATACTACACATATATTTTTAATTTATGTTCATGTTTGCACCAAAGCTTAATTTTATCAAAAAA

Coding sequence (CDS)

ATGGATTTCAGTCTCTACAACGTTTCCATGGAGGACAAGACAATCGGAATCGACCGCGCCGAAAATCCCTCTTTCCGCTTCCTCATCATGATCGATTGCTTCCGCTTCCAGAAACTCGCCTCCTGGATCTACGGCTTCGCCTCCAGAAACTACTCGCCTCTCCCCGCTTCGGAGACCCCGAGATCGGAAGACGCCGGCGGGAGGCTGAGCAGAGAGGAGATGAAGTCGGTGATGGGGAAATTAGGGCTCGTCTGCGGCGATGAATCGGAGGAATCGCTTCCTCTGTTTGGGTCGGTTGAAGAGGTGTGGAAGCTGTTCGAGGAAAGGGCGGCGTGTGCGGCGGAGGTGAAGGAGGCCTTCGATGTGTTCGATGTGAACGGCGATGGGTTCATCGATGTTGAAGAGTTGCAGAGAGTGCTGTGCGTGTTGGGGTTGAAGGAAGGGGAAGGGATTGGGAATTGCAGGAGGATGATCGAGAAGTTCGATGAGAATAAAGATGGGAGAATTGATTTTGAGGAGTTTGTGAAGTTGATGGAATCTGTGGTTTGA

Protein sequence

MDFSLYNVSMEDKTIGIDRAENPSFRFLIMIDCFRFQKLASWIYGFASRNYSPLPASETPRSEDAGGRLSREEMKSVMGKLGLVCGDESEESLPLFGSVEEVWKLFEERAACAAEVKEAFDVFDVNGDGFIDVEELQRVLCVLGLKEGEGIGNCRRMIEKFDENKDGRIDFEEFVKLMESVV

Homology

[Close]

BLAST of Lcy09g003720 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.0e-24
Identity = 67/123 (54.47%), Postives = 80/123 (65.04%), Query Frame = 0


Query: 58  ETPRSEDAGGRLSREEMKSVMGKLGLVCGDESEESLPLFGSVEEVWKLFEERAACAAEVK 117
           E  R +D  G L RE++  VM  LGL   D+  E L    S +EV  LFEE+     EVK
Sbjct: 81  EEYRDDDDDG-LCREDVGMVMKSLGL-STDQENEGLQKQYSSKEVSNLFEEKEPSLEEVK 140

Query: 118 EAFDVFDVNGDGFIDVEELQRVLCVLGLKEGEGIGNCRRMIEKFDENKDGRIDFEEFVKL 177
           +AFDVFD N DGFID  +LQRVL +LGLK+G  + NCRRMI  FD +KDGRIDF  FVK 
Sbjct: 141 QAFDVFDENRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGFVKF 200

Query: 178 MES 181
           ME+
Sbjct: 201 MEN 201

[Close]

BLAST of Lcy09g003720 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 6.8e-24
Identity = 62/112 (55.36%), Postives = 73/112 (65.18%), Query Frame = 0


Query: 69  LSREEMKSVMGKLGLVCGDESEESLPLFGSVEEVWKLFEERAACAAEVKEAFDVFDVNGD 128
           +SREE + VM  LGL   D   + L    S +EV  LFEE+ A   EVK+AFDVFD N D
Sbjct: 83  ISREEAEMVMRSLGLFYND---DQLQEQYSAKEVSSLFEEKEASLEEVKQAFDVFDENKD 142

Query: 129 GFIDVEELQRVLCVLGLKEGEGIGNCRRMIEKFDENKDGRIDFEEFVKLMES 181
           GFID  ELQRVL +LG K+G  + NC  MI   D NKDG+IDF EFVK ME+
Sbjct: 143 GFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMET 191

[Close]

BLAST of Lcy09g003720 vs. ExPASy Swiss-Prot
Match: Q0JC44 (Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 GN=CML22 PE=3 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 6.8e-16
Identity = 53/136 (38.97%), Postives = 75/136 (55.15%), Query Frame = 0


Query: 67  GRLSREEMKSVMGKLGL-VCGDESEESLPLFGS-------VEEVWKLF------------ 126
           GR++REE++  +GKLG+ V  DE    +    +       VEE  +L+            
Sbjct: 106 GRITREELEDSLGKLGIPVPADELAAVIARIDANGDGCVDVEEFGELYRSIMAGGDDSKD 165

Query: 127 ----EERAACAAEVKEAFDVFDVNGDGFIDVEELQRVLCVLGLKEGEGIGNCRRMIEKFD 179
               EE      +++EAF VFD NGDG+I V+EL  VL  LGLK+G     CRRMI + D
Sbjct: 166 GRAKEEEEEEDGDMREAFRVFDANGDGYITVDELGAVLASLGLKQGRTAEECRRMIGQVD 225

[Close]

BLAST of Lcy09g003720 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 4.9e-14
Identity = 46/124 (37.10%), Postives = 75/124 (60.48%), Query Frame = 0


Query: 67  GRLSREEMKSVMGKLGLVCGD----ESEESLPLFG----SVEEVWKLFE---ERAACAAE 126
           G+++++E+   +  LG+   D    +  E + L G     +EE   L++   E      +
Sbjct: 19  GKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGGLYQTIMEERDEEED 78

Query: 127 VKEAFDVFDVNGDGFIDVEELQRVLCVLGLKEGEGIGNCRRMIEKFDENKDGRIDFEEFV 180
           ++EAF+VFD N DGFI VEEL+ VL  LGLK+G  + +C+RMI K D + DG ++F+EF 
Sbjct: 79  MREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNFKEFK 138

[Close]

BLAST of Lcy09g003720 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 6.3e-14
Identity = 44/129 (34.11%), Postives = 80/129 (62.02%), Query Frame = 0


Query: 63  EDAGGRLSREEMKSVMGKLGLVCGD----ESEESLPLFG----SVEEVWKLF----EERA 122
           ++  G ++ +E+   +  LG+   D    +  E + + G     ++E  +L+    +E  
Sbjct: 15  KNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFGELYKTIMDEED 74

Query: 123 ACAAEVKEAFDVFDVNGDGFIDVEELQRVLCVLGLKEGEGIGNCRRMIEKFDENKDGRID 180
               ++KEAF+VFD NGDGFI V+EL+ VL  LGLK+G+ + +C++MI+K D + DGR++
Sbjct: 75  EEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDGRVN 134

[Close]

BLAST of Lcy09g003720 vs. ExPASy TrEMBL
Match: A0A6J1BVS4 (probable calcium-binding protein CML45 OS=Momordica charantia OX=3673 GN=LOC111006164 PE=4 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 1.0e-62
Identity = 130/188 (69.15%), Postives = 147/188 (78.19%), Query Frame = 0


Query: 1   MDFSLYNVSMEDKTIGIDRAENPSFRFLIMIDCFRFQKLASWI----YGFA----SRNYS 60
           MD +LYN+SM +KT   DR ENPS RFLIMIDC  FQKL SW+    +GFA    S   S
Sbjct: 1   MDLNLYNLSMAEKT---DRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCS 60

Query: 61  PLPASETPRSEDAGGRLSREEMKSVMGKLGLVCGDESEESLPLFGSVEEVWKLFEERAAC 120
           P+P +      D G  +SREEMKSVMG LG+VCG+E +ESLPLFGSVEE+ KLFEER  C
Sbjct: 61  PIPKAVV----DGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPC 120

Query: 121 AAEVKEAFDVFDVNGDGFIDVEELQRVLCVLGLKEGEGIGNCRRMIEKFDENKDGRIDFE 180
            AEVK AFDVFD NGDGFIDV ELQRVLCVLGLKEGEG+ NC+RMI+KFDEN+DG+IDFE
Sbjct: 121 IAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFE 180

[Close]

BLAST of Lcy09g003720 vs. ExPASy TrEMBL
Match: A0A6J1JZ70 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111489598 PE=4 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 2.6e-58
Identity = 123/166 (74.10%), Postives = 136/166 (81.93%), Query Frame = 0


Query: 22  NPSFRFLIMIDCFRFQKLASWIYGFAS--RNYSPLPASETPRSE---DAGGRLSREEMKS 81
           NPSFRFLIMIDCFRFQKL SW+Y FAS  R    LPASET +SE   + G R+SREEMK 
Sbjct: 9   NPSFRFLIMIDCFRFQKLGSWLYAFASGKRKQWSLPASETAKSEQRKEGGERVSREEMKW 68

Query: 82  VMGKLGLVCGDESEESLPLFGSVEEVWKLFEERAACAAEVKEAFDVFDVNGDGFIDVEEL 141
           VM +LGL  G+E+EESLP F S+EE+ K+F++ AA AAEVKEAFDVFDVN DGFIDVEEL
Sbjct: 69  VMEELGLSWGEETEESLPGFASMEEMCKIFDQTAASAAEVKEAFDVFDVNSDGFIDVEEL 128

Query: 142 QRVLCVLGLKEGEGIGNCRRMIEKFDENKDGRIDFEEFVKLMESVV 183
           QRVLCVLG KEGE I NCRRMI KFDEN DGRIDF+EFVKLMES V
Sbjct: 129 QRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMESAV 174

[Close]

BLAST of Lcy09g003720 vs. ExPASy TrEMBL
Match: A0A6J1FKT4 (probable calcium-binding protein CML45 OS=Cucurbita moschata OX=3662 GN=LOC111445127 PE=4 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 7.0e-56
Identity = 118/165 (71.52%), Postives = 134/165 (81.21%), Query Frame = 0


Query: 23  PSFRFLIMIDCFRFQKLASWIYGFAS--RNYSPLPASETPRSE---DAGGRLSREEMKSV 82
           PSFRFLIMIDCFRFQKL SW+Y FA+  R    LPASET +SE   + G R+SREEMK V
Sbjct: 10  PSFRFLIMIDCFRFQKLGSWLYAFATGKRKQWSLPASETAKSEQRDEGGERVSREEMKWV 69

Query: 83  MGKLGLVCGDESEESLPLFGSVEEVWKLFEERAACAAEVKEAFDVFDVNGDGFIDVEELQ 142
           MG+LGL   +E+EESLP F S+EE+ ++F++ AA AAEVKEAFDVFDVN DGFID +ELQ
Sbjct: 70  MGELGLSSEEETEESLPGFASMEEMCRIFDQTAASAAEVKEAFDVFDVNSDGFIDGDELQ 129

Query: 143 RVLCVLGLKEGEGIGNCRRMIEKFDENKDGRIDFEEFVKLMESVV 183
           RVLCVLG KEGE I NCRRMI KFDEN DGRIDF+EFVKLMES V
Sbjct: 130 RVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMESAV 174

[Close]

BLAST of Lcy09g003720 vs. ExPASy TrEMBL
Match: A0A1S3CAB3 (probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103498740 PE=4 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 7.7e-55
Identity = 122/187 (65.24%), Postives = 140/187 (74.87%), Query Frame = 0


Query: 1   MDFSLYNVSMEDKTIGIDRAENPSFRFLIMIDCFRFQKLASWIYGFASR--NYSPLPASE 60
           M+F LYNVSM+DK+      +NPSFRFLI+ID  RFQ LASW+  FAS     S LPAS 
Sbjct: 1   MNFGLYNVSMDDKS----TPQNPSFRFLIIIDFLRFQNLASWLCSFASTKLKQSTLPASS 60

Query: 61  TPRSEDAGGR---LSREEMKSVMGKLGLVCGDESEESLPLFGSVEEVWKLFEERAACAAE 120
                 AG R   +S EEMK VMG LG+ C + S  S+PLFGS EEV ++F++ AA AAE
Sbjct: 61  KSVESSAGERERLVSTEEMKWVMGNLGMDCSEGS--SVPLFGSAEEVAEMFDQTAASAAE 120

Query: 121 VKEAFDVFDVNGDGFIDVEELQRVLCVLGLKEGEGIGNCRRMIEKFDENKDGRIDFEEFV 180
           VK+AFDVFDVN DGFIDVEELQRV+CVLG KEGEGI NC +MI KFD NKDGRIDFEEFV
Sbjct: 121 VKQAFDVFDVNADGFIDVEELQRVMCVLGFKEGEGIENCEKMISKFDYNKDGRIDFEEFV 180

Query: 181 KLMESVV 183
           KLME+VV
Sbjct: 181 KLMEAVV 181

[Close]

BLAST of Lcy09g003720 vs. ExPASy TrEMBL
Match: A0A5A7T9C9 (Putative calcium-binding protein CML30 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002800 PE=4 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 4.0e-51
Identity = 116/178 (65.17%), Postives = 133/178 (74.72%), Query Frame = 0


Query: 10  MEDKTIGIDRAENPSFRFLIMIDCFRFQKLASWIYGFASR--NYSPLPASETPRSEDAGG 69
           M+DK+      +NPSFRFLI+ID  RFQ LASW+  FAS     S LPAS       AG 
Sbjct: 1   MDDKS----TPQNPSFRFLIIIDFLRFQNLASWLCSFASTKLKQSTLPASSKSVESSAGE 60

Query: 70  R---LSREEMKSVMGKLGLVCGDESEESLPLFGSVEEVWKLFEERAACAAEVKEAFDVFD 129
           R   +S EEMK VMG LG+ C + S  S+PLFGS EEV ++F++ AA AAEVK+AFDVFD
Sbjct: 61  RERLVSTEEMKWVMGNLGMDCSEGS--SVPLFGSAEEVAEMFDQTAASAAEVKQAFDVFD 120

Query: 130 VNGDGFIDVEELQRVLCVLGLKEGEGIGNCRRMIEKFDENKDGRIDFEEFVKLMESVV 183
           VNGDGFIDVEELQRV+CVLG KEGEGI NC +MI KFD NKDGRIDFEEFVKLME+VV
Sbjct: 121 VNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMISKFDYNKDGRIDFEEFVKLMEAVV 172

[Close]

BLAST of Lcy09g003720 vs. NCBI nr
Match: XP_022133625.1 (probable calcium-binding protein CML45 [Momordica charantia])

HSP 1 Score: 249.6 bits (636), Expect = 2.1e-62
Identity = 130/188 (69.15%), Postives = 147/188 (78.19%), Query Frame = 0


Query: 1   MDFSLYNVSMEDKTIGIDRAENPSFRFLIMIDCFRFQKLASWI----YGFA----SRNYS 60
           MD +LYN+SM +KT   DR ENPS RFLIMIDC  FQKL SW+    +GFA    S   S
Sbjct: 1   MDLNLYNLSMAEKT---DRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRNSNRCS 60

Query: 61  PLPASETPRSEDAGGRLSREEMKSVMGKLGLVCGDESEESLPLFGSVEEVWKLFEERAAC 120
           P+P +      D G  +SREEMKSVMG LG+VCG+E +ESLPLFGSVEE+ KLFEER  C
Sbjct: 61  PIPKAVV----DGGESVSREEMKSVMGDLGIVCGEEGDESLPLFGSVEEIAKLFEERGPC 120

Query: 121 AAEVKEAFDVFDVNGDGFIDVEELQRVLCVLGLKEGEGIGNCRRMIEKFDENKDGRIDFE 180
            AEVK AFDVFD NGDGFIDV ELQRVLCVLGLKEGEG+ NC+RMI+KFDEN+DG+IDFE
Sbjct: 121 IAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFE 180

[Close]

BLAST of Lcy09g003720 vs. NCBI nr
Match: XP_022993675.1 (probable calcium-binding protein CML30 [Cucurbita maxima])

HSP 1 Score: 235.0 bits (598), Expect = 5.3e-58
Identity = 123/166 (74.10%), Postives = 136/166 (81.93%), Query Frame = 0


Query: 22  NPSFRFLIMIDCFRFQKLASWIYGFAS--RNYSPLPASETPRSE---DAGGRLSREEMKS 81
           NPSFRFLIMIDCFRFQKL SW+Y FAS  R    LPASET +SE   + G R+SREEMK 
Sbjct: 9   NPSFRFLIMIDCFRFQKLGSWLYAFASGKRKQWSLPASETAKSEQRKEGGERVSREEMKW 68

Query: 82  VMGKLGLVCGDESEESLPLFGSVEEVWKLFEERAACAAEVKEAFDVFDVNGDGFIDVEEL 141
           VM +LGL  G+E+EESLP F S+EE+ K+F++ AA AAEVKEAFDVFDVN DGFIDVEEL
Sbjct: 69  VMEELGLSWGEETEESLPGFASMEEMCKIFDQTAASAAEVKEAFDVFDVNSDGFIDVEEL 128

Query: 142 QRVLCVLGLKEGEGIGNCRRMIEKFDENKDGRIDFEEFVKLMESVV 183
           QRVLCVLG KEGE I NCRRMI KFDEN DGRIDF+EFVKLMES V
Sbjct: 129 QRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMESAV 174

[Close]

BLAST of Lcy09g003720 vs. NCBI nr
Match: XP_038889669.1 (probable calcium-binding protein CML46 [Benincasa hispida])

HSP 1 Score: 232.3 bits (591), Expect = 3.4e-57
Identity = 121/185 (65.41%), Postives = 140/185 (75.68%), Query Frame = 0


Query: 1   MDFSLYNVSMEDKTIGIDRAENPSFRFLIMIDCFRFQKLASWIYGFASRNYSPLPASETP 60
           MDF +YNVSM+DKT      +NPSFRFLI+ID  RFQKLASW+Y FA+    P     T 
Sbjct: 1   MDFGVYNVSMDDKT----TTQNPSFRFLIVIDFLRFQKLASWLYAFATTKQKPSSFPATS 60

Query: 61  RSEDAGGR---LSREEMKSVMGKLGLVCGDESEESLPLFGSVEEVWKLFEERAACAAEVK 120
           ++E+ GG    ++ EEMK VMG LG+ CG   EES+PLFGS EEV ++F+ERAA   EVK
Sbjct: 61  KTEEHGGEERLMTTEEMKWVMGNLGIDCG---EESVPLFGSTEEVAEMFDERAASTVEVK 120

Query: 121 EAFDVFDVNGDGFIDVEELQRVLCVLGLKEGEGIGNCRRMIEKFDENKDGRIDFEEFVKL 180
           +AFDVFDVNGDGFID EELQRV+CVLG KEGE I NC RMI KFD N DGRIDFEEF+KL
Sbjct: 121 KAFDVFDVNGDGFIDSEELQRVMCVLGFKEGERIQNCERMIRKFDHNGDGRIDFEEFLKL 178

Query: 181 MESVV 183
           MESVV
Sbjct: 181 MESVV 178

[Close]

BLAST of Lcy09g003720 vs. NCBI nr
Match: KAG6578574.1 (putative calcium-binding protein CML46, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 228.8 bits (582), Expect = 3.8e-56
Identity = 119/166 (71.69%), Postives = 135/166 (81.33%), Query Frame = 0


Query: 22  NPSFRFLIMIDCFRFQKLASWIYGFAS--RNYSPLPASETPRSE---DAGGRLSREEMKS 81
           NPSFRFLIMIDCFRFQKL SW+Y FA+  R    LPASET +SE   + G R+SREEMK 
Sbjct: 9   NPSFRFLIMIDCFRFQKLGSWLYAFATGKRKQWSLPASETGKSEQRDEGGERVSREEMKW 68

Query: 82  VMGKLGLVCGDESEESLPLFGSVEEVWKLFEERAACAAEVKEAFDVFDVNGDGFIDVEEL 141
           VMG+LGL   +E+EESLP F S+EE+ ++F++ AA AAEVKEAFDVFDVN DGFID +EL
Sbjct: 69  VMGELGLSSEEETEESLPGFASMEEMCRIFDQTAASAAEVKEAFDVFDVNSDGFIDGDEL 128

Query: 142 QRVLCVLGLKEGEGIGNCRRMIEKFDENKDGRIDFEEFVKLMESVV 183
           QRVLCVLG KEGE I NCRRMI KFDEN DGRIDF+EFVKLMES V
Sbjct: 129 QRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMESAV 174

[Close]

BLAST of Lcy09g003720 vs. NCBI nr
Match: XP_022939128.1 (probable calcium-binding protein CML45 [Cucurbita moschata])

HSP 1 Score: 226.9 bits (577), Expect = 1.4e-55
Identity = 118/165 (71.52%), Postives = 134/165 (81.21%), Query Frame = 0


Query: 23  PSFRFLIMIDCFRFQKLASWIYGFAS--RNYSPLPASETPRSE---DAGGRLSREEMKSV 82
           PSFRFLIMIDCFRFQKL SW+Y FA+  R    LPASET +SE   + G R+SREEMK V
Sbjct: 10  PSFRFLIMIDCFRFQKLGSWLYAFATGKRKQWSLPASETAKSEQRDEGGERVSREEMKWV 69

Query: 83  MGKLGLVCGDESEESLPLFGSVEEVWKLFEERAACAAEVKEAFDVFDVNGDGFIDVEELQ 142
           MG+LGL   +E+EESLP F S+EE+ ++F++ AA AAEVKEAFDVFDVN DGFID +ELQ
Sbjct: 70  MGELGLSSEEETEESLPGFASMEEMCRIFDQTAASAAEVKEAFDVFDVNSDGFIDGDELQ 129

Query: 143 RVLCVLGLKEGEGIGNCRRMIEKFDENKDGRIDFEEFVKLMESVV 183
           RVLCVLG KEGE I NCRRMI KFDEN DGRIDF+EFVKLMES V
Sbjct: 130 RVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMESAV 174

[Close]

BLAST of Lcy09g003720 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 114.8 bits (286), Expect = 7.4e-26
Identity = 67/123 (54.47%), Postives = 80/123 (65.04%), Query Frame = 0


Query: 58  ETPRSEDAGGRLSREEMKSVMGKLGLVCGDESEESLPLFGSVEEVWKLFEERAACAAEVK 117
           E  R +D  G L RE++  VM  LGL   D+  E L    S +EV  LFEE+     EVK
Sbjct: 81  EEYRDDDDDG-LCREDVGMVMKSLGL-STDQENEGLQKQYSSKEVSNLFEEKEPSLEEVK 140

Query: 118 EAFDVFDVNGDGFIDVEELQRVLCVLGLKEGEGIGNCRRMIEKFDENKDGRIDFEEFVKL 177
           +AFDVFD N DGFID  +LQRVL +LGLK+G  + NCRRMI  FD +KDGRIDF  FVK 
Sbjct: 141 QAFDVFDENRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGFVKF 200

Query: 178 MES 181
           ME+
Sbjct: 201 MEN 201

[Close]

BLAST of Lcy09g003720 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 112.1 bits (279), Expect = 4.8e-25
Identity = 62/112 (55.36%), Postives = 73/112 (65.18%), Query Frame = 0


Query: 69  LSREEMKSVMGKLGLVCGDESEESLPLFGSVEEVWKLFEERAACAAEVKEAFDVFDVNGD 128
           +SREE + VM  LGL   D   + L    S +EV  LFEE+ A   EVK+AFDVFD N D
Sbjct: 83  ISREEAEMVMRSLGLFYND---DQLQEQYSAKEVSSLFEEKEASLEEVKQAFDVFDENKD 142

Query: 129 GFIDVEELQRVLCVLGLKEGEGIGNCRRMIEKFDENKDGRIDFEEFVKLMES 181
           GFID  ELQRVL +LG K+G  + NC  MI   D NKDG+IDF EFVK ME+
Sbjct: 143 GFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMET 191

[Close]

BLAST of Lcy09g003720 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 79.3 bits (194), Expect = 3.5e-15
Identity = 46/124 (37.10%), Postives = 75/124 (60.48%), Query Frame = 0


Query: 67  GRLSREEMKSVMGKLGLVCGD----ESEESLPLFG----SVEEVWKLFE---ERAACAAE 126
           G+++++E+   +  LG+   D    +  E + L G     +EE   L++   E      +
Sbjct: 19  GKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGGLYQTIMEERDEEED 78

Query: 127 VKEAFDVFDVNGDGFIDVEELQRVLCVLGLKEGEGIGNCRRMIEKFDENKDGRIDFEEFV 180
           ++EAF+VFD N DGFI VEEL+ VL  LGLK+G  + +C+RMI K D + DG ++F+EF 
Sbjct: 79  MREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNFKEFK 138

[Close]

BLAST of Lcy09g003720 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 79.0 bits (193), Expect = 4.5e-15
Identity = 44/129 (34.11%), Postives = 80/129 (62.02%), Query Frame = 0


Query: 63  EDAGGRLSREEMKSVMGKLGLVCGD----ESEESLPLFG----SVEEVWKLF----EERA 122
           ++  G ++ +E+   +  LG+   D    +  E + + G     ++E  +L+    +E  
Sbjct: 15  KNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFGELYKTIMDEED 74

Query: 123 ACAAEVKEAFDVFDVNGDGFIDVEELQRVLCVLGLKEGEGIGNCRRMIEKFDENKDGRID 180
               ++KEAF+VFD NGDGFI V+EL+ VL  LGLK+G+ + +C++MI+K D + DGR++
Sbjct: 75  EEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDGRVN 134

[Close]

BLAST of Lcy09g003720 vs. TAIR 10
Match: AT3G59440.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 77.8 bits (190), Expect = 1.0e-14
Identity = 45/136 (33.09%), Postives = 78/136 (57.35%), Query Frame = 0


Query: 63  EDAGGRLSREEMKSVMGKLGLVCGDES------------------EESLPLFGSVEEVWK 122
           ++  GR+++EE+   +  LG+   D+                    E   L+GS+     
Sbjct: 61  KNGDGRITKEELNDSLENLGIFMPDKDLIQMIQKMDANGDGCVDINEFESLYGSI----- 120

Query: 123 LFEERAACAAEVKEAFDVFDVNGDGFIDVEELQRVLCVLGLKEGEGIGNCRRMIEKFDEN 181
             EE+     ++++AF+VFD +GDGFI VEEL  V+  LGLK+G+ +  C+ MI + DE+
Sbjct: 121 -VEEKE--EGDMRDAFNVFDQDGDGFITVEELNSVMTSLGLKQGKTLECCKEMIMQVDED 180

The following BLAST results are available for this feature:

BLAST of Lcy09g003720 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93Z27	1.0e-24	54.47	Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ...	[more]
Q9MBG5	6.8e-24	55.36	Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ...	[more]
Q0JC44	6.8e-16	38.97	Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9SRR7	4.9e-14	37.10	Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1	[more]
Q9LNE7	6.3e-14	34.11	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]

BLAST of Lcy09g003720 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BVS4	1.0e-62	69.15	probable calcium-binding protein CML45 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1JZ70	2.6e-58	74.10	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114895...	[more]
A0A6J1FKT4	7.0e-56	71.52	probable calcium-binding protein CML45 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A1S3CAB3	7.7e-55	65.24	probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103498740 P...	[more]
A0A5A7T9C9	4.0e-51	65.17	Putative calcium-binding protein CML30 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of Lcy09g003720 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133625.1	2.1e-62	69.15	probable calcium-binding protein CML45 [Momordica charantia]	[more]
XP_022993675.1	5.3e-58	74.10	probable calcium-binding protein CML30 [Cucurbita maxima]	[more]
XP_038889669.1	3.4e-57	65.41	probable calcium-binding protein CML46 [Benincasa hispida]	[more]
KAG6578574.1	3.8e-56	71.69	putative calcium-binding protein CML46, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022939128.1	1.4e-55	71.52	probable calcium-binding protein CML45 [Cucurbita moschata]	[more]

BLAST of Lcy09g003720 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G39670.1	7.4e-26	54.47	Calcium-binding EF-hand family protein	[more]
AT3G29000.1	4.8e-25	55.36	Calcium-binding EF-hand family protein	[more]
AT3G07490.1	3.5e-15	37.10	ARF-GAP domain 11	[more]
AT1G05990.1	4.5e-15	34.11	EF hand calcium-binding protein family	[more]
AT3G59440.1	1.0e-14	33.09	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 115..143 e-value: 1.3E-5 score: 34.6 coord: 153..181 e-value: 4.4E-6 score: 36.2
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 116..179 e-value: 9.8E-14 score: 51.6
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 111..146 score: 15.489744
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 149..182 score: 14.5413
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 115..178 e-value: 5.57424E-18 score: 71.8101
None	No IPR available	GENE3D	1.10.238.10		coord: 59..182 e-value: 3.6E-23 score: 84.2
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 63..180
None	No IPR available	PANTHER	PTHR10891:SF805	CALCIUM-BINDING PROTEIN CML45-RELATED	coord: 63..180
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 124..136
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 162..174
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 64..179

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy09g003720.1	Lcy09g003720.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding