Lcy09g000310 (gene) Sponge gourd (P93075) v1

Overview
NameLcy09g000310
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
LocationChr09: 298867 .. 301608 (-)
RNA-Seq ExpressionLcy09g000310
SyntenyLcy09g000310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTAGGATGGTAGTTAGAGAGCTCATTTTAAATTGCGTCGGCCTAACAAAATAGCATTGAGTATAAGACACCTTAGCTCTTCTCTATAAACAAGATAAGGAGTTTTGGAAGCTTAGATTAAAGTGTATCTATTGTACTACACAATTAGAATCGTCCTTCTCAAATTACTCTCTCTAAAAGATATTTATAAGTATCATTAAAAAAGAACATAATTGTATAAGTAAATCAAAACTAAAGTATAACAAATGGTTTCTAAATTTCGAATAGTGACCAAATAAATGTTAAAAGAAATTTGAATTCAAATAAATCCCAACTAGATAGATTGTAAGGTTCTATATATCAAAGCATCATTCCATTTTTGGAATATATATTTTCTATCATCATGATTGCTCAATACTTCCTACGAGAAAATAAGTAGGACTAATCATTCCTACGAGAAAATAAGTAGGACTAGCCAATCTGAATATTGCTCAACAAATAAGCTACCTATTATTGTCTTACAAGTTCATGGCTCCATCTCCTACCCCCATGTAACGCCCCAGGCCCAGGATTCGGAATCCGAATTCGGCCTTTAACGGCCCCGACATTCCCCTGCGACCTGGCCACGCCACCATACTTGTCTGATACTACTTCTAAGATTGAAGATTGTTCCCACAAACCAACACGGGGTCATTTTAGCATGCTTTGTCCTCACCCACATGCTTCCTAGGAAAATTTCCAGGAGGCCACCCAACATAGAACTTCTCCAAGCTAAGCACGTTTAACTTCAAAGTTCCTATGATTGAGCCACCGAAAAGGAAGGTGCACCTTGTTGGTATAGGTAGTATCTATCAATTCTTTTAAGCTTATCTTGACCATACTTTCATGTCCTCAGGACCCTCTCATTCGGATGTGGTATCGGTTCATTCATGTACCCCTTCTAACCTCGGGCGTCACACCCATAATCATTAACCTAAAAACAATGGCTAACGTGACACTTTTTAAAGATAAAATAAAAATTTTCCAAAATTAAAATAATTATAACTAATATTAAAATAATTAAAAAAATAAAAACTCCATGGTGATACATGGTTTACAATGATGTGCAATTACCTCCAAAGCCCCACTTGGCACTTAGCTTTCATAAGACTGCAGCTGTGCACTGATCCAAGGTATGTTTGGTTTAACTTTTTAAGTGTTTAATTTTGAAAATAAATCATTTTGAAAAAAATATAGTGTTTGGCAACTACTAAAAATGACTTATTAGAAAATGAGATTGATAAATAAACCATTTTGAGATAAACATTTGAAACCAACTTTTTAAAAAAGACTTCTTAAGTAAACAATAACTTGTGTAGAAAAACATTTGTGAGGAAATGCAACCAAACATATGAGTGTTTAAATTCTAAACAATTTTTATAAAAAATGTTTTTTAAAAAATGCATTATTGAAAAACATTTAAATTAAAATGTATTCCAAACGGATCCCAATTCCTATTGATGACTGATGAGCACCTTCCACTCATTTTTTCCCACGTTTATGGCGTTTTTTGTTTTGTTTTGTTTTTGTATTTATTTTTTTATTTTTTTATTTTTACAATCGTTTTTCTTCTATTTTTCATGACCCTTCCTCAAAGATAATTTAATGATAAGATACCCCACATCTTATCTATAAGGAAGGGTGGAGGGATAATGCGAGAAAAGCATGCACCCTAATTGATCCTTTGGAATGGCTTTACACCGGCAACGTTAACGCACAATCTCCCACCCTTCCATGGAAGCCCAAGCTTCTCAATCTTTTGTTTTCCATCCTTTTTCTACAACCTTTCCGATCAGATTTTGCCGTCAATTGCAGCCGCCTCCGCCTTCCACACCATAAACTTCCTCCTTCTCACGGCGTCGCCTCCTTCGACACCGTCATTGATTCTTCCACCCCCGACCTCTGGATTCGCGTTTATAAACCTCTCACATTTTCCGACGCCGATCCTTTGCCTGTTTTAATATACTTCCACGGCGGAGGCTTTGTTTCCGGCTCCCCCGATTGGCCTCTGATCGACAACTTCTCTCGCGATTTTGCACGCGAACTCGGAGCCATCCTCGTCTCCGTCAATTACCGACTCGCGCCGGAGCACCGATTTCCGAGCCAATACGACGACGGATTGCACGTTCTCAAGGTAATCGACGAACGACGAATATCCGACGAAACTCTTCCTAAAAACGCCGATTTGAGCCGCTGTTTCATTGTCGGCGAAAGCGCCGGAGGAAACATAGCGCATCACTTGACGGTGAGAGCGACGACATACCAGTTCAAGAGGCTTAAAATCGTAGGTCTGATTGCGATTCAGCCATTTTTCGGAGGAGAAAAGCGGACAAGTTCAGAGCTTCGATTTGGGCGCGGTGGTTATGGACTGACATTGGAAAAGACGGATTCGCATTGGAAGGCCTTTTTGCCGATTGGTTCGAACCGCGATCACCCGGCCGCTAACGTGGTTCGGTCCAAAATTCCCGGCTTGAATTTTCCACTGGCGCTTGTTGTGATTGGCGGTTGGGATCCATTAAGCGATCGGGATAAAGAGTACATCGAGTGGCTGAAGAAATCGGGTAACGAGGTTCGGGTCGTGGAGTATCCGAATGCGATCCATGGGTTCAATTGGATCCCGGATTTTCCTGAATATTCCATGCTGATGCAAGACGCTAAAGAATTCGTGAAGAATATTAATGCAACAGGTTTATCGTCGCAAGAACCACGTTCATTCATTTGA

mRNA sequence

ATGTTAGGATGCCGCCTCCGCCTTCCACACCATAAACTTCCTCCTTCTCACGGCGTCGCCTCCTTCGACACCGTCATTGATTCTTCCACCCCCGACCTCTGGATTCGCGTTTATAAACCTCTCACATTTTCCGACGCCGATCCTTTGCCTGTTTTAATATACTTCCACGGCGGAGGCTTTGTTTCCGGCTCCCCCGATTGGCCTCTGATCGACAACTTCTCTCGCGATTTTGCACGCGAACTCGGAGCCATCCTCGTCTCCGTCAATTACCGACTCGCGCCGGAGCACCGATTTCCGAGCCAATACGACGACGGATTGCACGTTCTCAAGGTAATCGACGAACGACGAATATCCGACGAAACTCTTCCTAAAAACGCCGATTTGAGCCGCTGTTTCATTGTCGGCGAAAGCGCCGGAGGAAACATAGCGCATCACTTGACGGTGAGAGCGACGACATACCAGTTCAAGAGGCTTAAAATCGTAGGTCTGATTGCGATTCAGCCATTTTTCGGAGGAGAAAAGCGGACAAGTTCAGAGCTTCGATTTGGGCGCGGTGGTTATGGACTGACATTGGAAAAGACGGATTCGCATTGGAAGGCCTTTTTGCCGATTGGTTCGAACCGCGATCACCCGGCCGCTAACGTGGTTCGGTCCAAAATTCCCGGCTTGAATTTTCCACTGGCGCTTGTTGTGATTGGCGGTTGGGATCCATTAAGCGATCGGGATAAAGAGTACATCGAGTGGCTGAAGAAATCGGGTAACGAGGTTCGGGTCGTGGAGTATCCGAATGCGATCCATGGGTTCAATTGGATCCCGGATTTTCCTGAATATTCCATGCTGATGCAAGACGCTAAAGAATTCGTGAAGAATATTAATGCAACAGGTTTATCGTCGCAAGAACCACGTTCATTCATTTGA

Coding sequence (CDS)

ATGTTAGGATGCCGCCTCCGCCTTCCACACCATAAACTTCCTCCTTCTCACGGCGTCGCCTCCTTCGACACCGTCATTGATTCTTCCACCCCCGACCTCTGGATTCGCGTTTATAAACCTCTCACATTTTCCGACGCCGATCCTTTGCCTGTTTTAATATACTTCCACGGCGGAGGCTTTGTTTCCGGCTCCCCCGATTGGCCTCTGATCGACAACTTCTCTCGCGATTTTGCACGCGAACTCGGAGCCATCCTCGTCTCCGTCAATTACCGACTCGCGCCGGAGCACCGATTTCCGAGCCAATACGACGACGGATTGCACGTTCTCAAGGTAATCGACGAACGACGAATATCCGACGAAACTCTTCCTAAAAACGCCGATTTGAGCCGCTGTTTCATTGTCGGCGAAAGCGCCGGAGGAAACATAGCGCATCACTTGACGGTGAGAGCGACGACATACCAGTTCAAGAGGCTTAAAATCGTAGGTCTGATTGCGATTCAGCCATTTTTCGGAGGAGAAAAGCGGACAAGTTCAGAGCTTCGATTTGGGCGCGGTGGTTATGGACTGACATTGGAAAAGACGGATTCGCATTGGAAGGCCTTTTTGCCGATTGGTTCGAACCGCGATCACCCGGCCGCTAACGTGGTTCGGTCCAAAATTCCCGGCTTGAATTTTCCACTGGCGCTTGTTGTGATTGGCGGTTGGGATCCATTAAGCGATCGGGATAAAGAGTACATCGAGTGGCTGAAGAAATCGGGTAACGAGGTTCGGGTCGTGGAGTATCCGAATGCGATCCATGGGTTCAATTGGATCCCGGATTTTCCTGAATATTCCATGCTGATGCAAGACGCTAAAGAATTCGTGAAGAATATTAATGCAACAGGTTTATCGTCGCAAGAACCACGTTCATTCATTTGA

Protein sequence

MLGCRLRLPHHKLPPSHGVASFDTVIDSSTPDLWIRVYKPLTFSDADPLPVLIYFHGGGFVSGSPDWPLIDNFSRDFARELGAILVSVNYRLAPEHRFPSQYDDGLHVLKVIDERRISDETLPKNADLSRCFIVGESAGGNIAHHLTVRATTYQFKRLKIVGLIAIQPFFGGEKRTSSELRFGRGGYGLTLEKTDSHWKAFLPIGSNRDHPAANVVRSKIPGLNFPLALVVIGGWDPLSDRDKEYIEWLKKSGNEVRVVEYPNAIHGFNWIPDFPEYSMLMQDAKEFVKNINATGLSSQEPRSFI
Homology
BLAST of Lcy09g000310 vs. ExPASy Swiss-Prot
Match: Q9LT10 (Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 2.8e-67
Identity = 137/293 (46.76%), Postives = 183/293 (62.46%), Query Frame = 0

Query: 6   LRLPHHKLPPS----HGVASFDTVIDSSTPDLWIRVYKPLTFSDADPLPVLIYFHGGGFV 65
           LRL   + PP+    + V++ D V+D S  DLW R+Y P      D +PV+++FHGGGF 
Sbjct: 43  LRLFDFRAPPNPKPVNIVSTSDFVVDQSR-DLWFRLYTP--HVSGDKIPVVVFFHGGGFA 102

Query: 66  SGSPDWPLIDNFSRDFARELGAILVSVNYRLAPEHRFPSQYDDGLHVLKVIDERRISDET 125
             SP+    DN  R FAR+L A ++SVNYRLAPEHR+P+QYDDG   LK I+E   S   
Sbjct: 103 FLSPNAYPYDNVCRRFARKLPAYVISVNYRLAPEHRYPAQYDDGFDALKYIEENHGS--I 162

Query: 126 LPKNADLSRCFIVGESAGGNIAHHLTV---RATTYQFKRLKIVGLIAIQPFFGGEKRTSS 185
           LP NADLSRCF  G+SAGGNIAH++ +   R     F  +K++GLI+IQPFFGGE+RT +
Sbjct: 163 LPANADLSRCFFAGDSAGGNIAHNVAIRICREPRSSFTAVKLIGLISIQPFFGGEERTEA 222

Query: 186 ELRFGRGGYGLTLEKTDSHWKAFLPIGSNRDHPAANVVRSK---IPGLNFPLALVVIGGW 245
           E +   G   ++ ++TD  WKA   +G NRDH A NV       I GL++P  +VV+ G+
Sbjct: 223 EKQL-VGAPLVSPDRTDWCWKA---MGLNRDHEAVNVGGPNAVDISGLDYPETMVVVAGF 282

Query: 246 DPLSDRDKEYIEWLKKSGNEVRVVEYPNAIHGFNWIPDFPEYSMLMQDAKEFV 289
           DPL D  + Y EWLK  G +  ++EYPN  H F   P+ PE   L+   K+FV
Sbjct: 283 DPLKDWQRSYYEWLKLCGKKATLIEYPNMFHAFYIFPELPEAGQLIMRIKDFV 326

BLAST of Lcy09g000310 vs. ExPASy Swiss-Prot
Match: Q940G6 (Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 2.4e-50
Identity = 113/289 (39.10%), Postives = 162/289 (56.06%), Query Frame = 0

Query: 15  PSHGVASFDTVIDSSTPDLWIRVYKPLTFS------------DADPLPVLIYFHGGGFVS 74
           P +GV SFD +ID  T +L  RVY+P                D + +PV+++FHGG F  
Sbjct: 59  PVNGVFSFDVIIDRQT-NLLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAH 118

Query: 75  GSPDWPLIDNFSRDFARELGAILVSVNYRLAPEHRFPSQYDDGLHVLKVIDERRISDETL 134
            S +  + D   R      GA++VSVNYR APE+R+P  YDDG  VLK ++    S    
Sbjct: 119 SSANSAIYDTLCRRLVGLCGAVVVSVNYRRAPENRYPCAYDDGWAVLKWVNS---SSWLR 178

Query: 135 PKNADLSRCFIVGESAGGNIAHHLTVRATTYQFKRLKIVGLIAIQPFFGGEKRTSSELRF 194
            K     R F+ G+S+GGNI H++ VRA      R+ ++G I + P FGG +RT SE R 
Sbjct: 179 SKKDSKVRIFLAGDSSGGNIVHNVAVRAVE---SRIDVLGNILLNPMFGGTERTESEKRL 238

Query: 195 GRGGYGLTLEKTDSHWKAFLPIGSNRDHPAANVV--RSK-IPGLNFPLALVVIGGWDPLS 254
             G Y +T+   D +W+AFLP G +R+HPA +    RSK + GL+FP +LVV+ G D + 
Sbjct: 239 D-GKYFVTVRDRDWYWRAFLPEGEDREHPACSPFGPRSKSLEGLSFPKSLVVVAGLDLIQ 298

Query: 255 DRDKEYIEWLKKSGNEVRVVEYPNAIHGFNWIPDFPEYSMLMQDAKEFV 289
           D   +Y E LKK+G EV+++    A  GF  +P+   +  +M +   FV
Sbjct: 299 DWQLKYAEGLKKAGQEVKLLYLEQATIGFYLLPNNNHFHTVMDEIAAFV 339

BLAST of Lcy09g000310 vs. ExPASy Swiss-Prot
Match: Q9LYC1 (Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 3.2e-50
Identity = 109/295 (36.95%), Postives = 165/295 (55.93%), Query Frame = 0

Query: 15  PSHGVASFDTVIDSSTPDLWIRVYKPLT---------------FSDADPLPVLIYFHGGG 74
           P  GV SFD V   ST +L  R+Y+P +                S  + +PVLI+FHGG 
Sbjct: 59  PLDGVFSFDHV--DSTTNLLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGS 118

Query: 75  FVSGSPDWPLIDNFSRDFARELGAILVSVNYRLAPEHRFPSQYDDGLHVLKVIDERRISD 134
           F   S +  + D F R      G ++VSV+YR +PEHR+P  YDDG + L  +  R    
Sbjct: 119 FTHSSANSAIYDTFCRRLVTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWVKSRVWLQ 178

Query: 135 ETLPKNADLSRCFIVGESAGGNIAHHLTVRATTYQFKRLKIVGLIAIQPFFGGEKRTSSE 194
                N  +   ++ G+S+GGNIAH++ VRAT    + +K++G I + P FGG++RT SE
Sbjct: 179 SGKDSNVYV---YLAGDSSGGNIAHNVAVRATN---EGVKVLGNILLHPMFGGQERTQSE 238

Query: 195 LRFGRGGYGLTLEKTDSHWKAFLPIGSNRDHPAANVVRSK---IPGLNFPLALVVIGGWD 254
                G Y +T++  D +W+A+LP G +RDHPA N    +   + G+NFP +LVV+ G D
Sbjct: 239 KTLD-GKYFVTIQDRDWYWRAYLPEGEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLD 298

Query: 255 PLSDRDKEYIEWLKKSGNEVRVVEYPNAIHGFNWIPDFPEYSMLMQDAKEFVKNI 292
            + D    Y++ LKK+G EV ++    A  GF ++P+   +  LM++  +FV +I
Sbjct: 299 LVQDWQLAYVDGLKKTGLEVNLLYLKQATIGFYFLPNNDHFHCLMEELNKFVHSI 344

BLAST of Lcy09g000310 vs. ExPASy Swiss-Prot
Match: Q6L545 (Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 5.4e-50
Identity = 111/299 (37.12%), Postives = 163/299 (54.52%), Query Frame = 0

Query: 15  PSHGVASFDTVIDSSTPDLWIRVYKPLTFSD---------------------ADPLPVLI 74
           P  GV+SFD +ID S   L +R+Y+     D                     A+P PV+I
Sbjct: 59  PLEGVSSFDHIIDQSV-GLEVRIYRAAAEGDAEEGAAAVTRPILEFLTDAPAAEPFPVII 118

Query: 75  YFHGGGFVSGSPDWPLIDNFSRDFARELGAILVSVNYRLAPEHRFPSQYDDGLHVLKVID 134
           +FHGG FV  S    + D+  R F +    ++VSVNYR APEHR+P  YDDG   LK + 
Sbjct: 119 FFHGGSFVHSSASSTIYDSLCRRFVKLSKGVVVSVNYRRAPEHRYPCAYDDGWTALKWV- 178

Query: 135 ERRISDETLPKNADL-SRCFIVGESAGGNIAHHLTVRATTYQFKRLKIVGLIAIQPFFGG 194
              +S   +    D  +R F+ G+S+GGNIAHH+ VRA     + +K+ G I +   FGG
Sbjct: 179 ---MSQPFMRSGGDAQARVFLSGDSSGGNIAHHVAVRAAD---EGVKVCGNILLNAMFGG 238

Query: 195 EKRTSSELRFGRGGYGLTLEKTDSHWKAFLPIGSNRDHPAANVV---RSKIPGLNFPLAL 254
            +RT SE R   G Y +TL+  D +WKA+LP  ++RDHPA N       ++ GL F  +L
Sbjct: 239 TERTESERRLD-GKYFVTLQDRDWYWKAYLPEDADRDHPACNPFGPNGRRLGGLPFAKSL 298

Query: 255 VVIGGWDPLSDRDKEYIEWLKKSGNEVRVVEYPNAIHGFNWIPDFPEYSMLMQDAKEFV 289
           +++ G D   DR   Y + L++ G+ V+VV+  NA  GF  +P+   Y  +M++  +F+
Sbjct: 299 IIVSGLDLTCDRQLAYADALREDGHHVKVVQCENATVGFYLLPNTVHYHEVMEEISDFL 348

BLAST of Lcy09g000310 vs. ExPASy Swiss-Prot
Match: Q9FG13 (Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 6.2e-46
Identity = 103/273 (37.73%), Postives = 155/273 (56.78%), Query Frame = 0

Query: 32  DLWIRVYKPLTFSDADPLPVLIYFHGGGFVSGSPDWPLIDNFSRDFARELGAILVSVNYR 91
           +L +R+YKP++ S+   LPV+++FHGGGF  GS  WP   NF    A  L A++VS +YR
Sbjct: 59  NLHLRLYKPISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYR 118

Query: 92  LAPEHRFPSQYDDGLHVLKVIDERRISDETL-----PKNADLSRCFIVGESAGGNIAHHL 151
           LAPEHR P+ ++D   VL  + ++ +SD          + D  R F+VG+S+GGNIAH L
Sbjct: 119 LAPEHRLPAAFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQL 178

Query: 152 TVR--ATTYQFKRLKIVGLIAIQPFFGGEKRTSSELRFGRGGYGLTLEKTDSHWKAFLPI 211
            VR  + + +   +++ G + + PFFGGE+RT+SE   G     L+L+  D  W+  LP 
Sbjct: 179 AVRFGSGSIELTPVRVRGYVLMGPFFGGEERTNSE--NGPSEALLSLDLLDKFWRLSLPN 238

Query: 212 GSNRDHPAANVVRSKIP---GLNFPLALVVIGGWDPLSDRDKEYIEWLKK-SGNEVRVVE 271
           G+ RDH  AN      P    ++    LV++GG + L DR KEY   LKK  G  V  +E
Sbjct: 239 GATRDHHMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIE 298

Query: 272 YPNAIHGF-NWIPDFPEYSMLMQDAKEFVKNIN 293
           + N  HGF +  P       +++   +F+ N++
Sbjct: 299 FENKEHGFYSNYPSSEAAEQVLRIIGDFMNNLS 329

BLAST of Lcy09g000310 vs. ExPASy TrEMBL
Match: A0A6J1HPS2 (probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111464932 PE=4 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 6.9e-117
Identity = 207/289 (71.63%), Postives = 236/289 (81.66%), Query Frame = 0

Query: 6   LRLPHHKLPPS---HGVASFDTVIDSSTPDLWIRVYKPLTFSDADPLPVLIYFHGGGFVS 65
           LRL   K+PPS    GVASFDT +DSS+ DLW+RVY PLT S++D +PV+IYFHGGGFV 
Sbjct: 46  LRLLDRKVPPSSTTRGVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVF 105

Query: 66  GSPDWPLIDNFSRDFARELGAILVSVNYRLAPEHRFPSQYDDGLHVLKVIDERRISDETL 125
           GS D   ID+FSRDFARE+GAI+VSVNYRLAPEHRFPS YDDG  VLK IDE  IS ++L
Sbjct: 106 GSADSQKIDSFSRDFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIS-KSL 165

Query: 126 PKNADLSRCFIVGESAGGNIAHHLTVRATTYQFKRLKIVGLIAIQPFFGGEKRTSSELRF 185
           P+NAD+S CFI GESAGGNI HH+TVRA  Y+FKRLKI G+IAIQPF GGE+RT SE+RF
Sbjct: 166 PENADISSCFIAGESAGGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRF 225

Query: 186 GRGGYGLTLEKTDSHWKAFLPIGSNRDHPAANVVRSKIPGLNFPLALVVIGGWDPLSDRD 245
           G+GG GLTLE TD +WKAFLPIGS+RDHP ANVVRS + GL  P ALVV GG D LSDR 
Sbjct: 226 GKGG-GLTLEMTDWYWKAFLPIGSSRDHPGANVVRSDVSGLKLPPALVVNGGLDLLSDRI 285

Query: 246 KEYIEWLKKSGNEVRVVEYPNAIHGFNWIPDFPEYSMLMQDAKEFVKNI 292
           KEY EWL+KSG EVRVVEYPN IHGF+W  D PEYSMLMQDA+EF + +
Sbjct: 286 KEYCEWLEKSGKEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRV 332

BLAST of Lcy09g000310 vs. ExPASy TrEMBL
Match: A0A0A0LMQ6 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G401430 PE=4 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 5.0e-115
Identity = 206/289 (71.28%), Postives = 234/289 (80.97%), Query Frame = 0

Query: 6   LRLPHHKLPP--SHGVASFDTVIDSSTPDLWIRVYKPLTFSDADPLPVLIYFHGGGFVSG 65
           LRL   KLPP  S GVA+ D  IDSST DLWIRVY PLTFS++DPLPV+IYFHGGGFV G
Sbjct: 47  LRLFDRKLPPFTSRGVAASDATIDSSTSDLWIRVYNPLTFSNSDPLPVIIYFHGGGFVYG 106

Query: 66  SPDWPLIDNFSRDFARELGAILVSVNYRLAPEHRFPSQYDDGLHVLKVIDERRISDETLP 125
           S D P  D F RDFARE+GAI++SVNYRLAPE RFPSQ+DDG HVLK +D+  IS ET+P
Sbjct: 107 SADAPPTDTFCRDFAREIGAIVISVNYRLAPEDRFPSQFDDGFHVLKAMDKGAIS-ETVP 166

Query: 126 KNADLSRCFIVGESAGGNIAHHLTVRATTYQFKRLKIVGLIAIQPFFGGEKRTSSELRFG 185
           +NADL RCFI GESAGGNIAHH+TVRA   +FKR+KIVG+I IQPFFGGE+R  SE+RFG
Sbjct: 167 ENADLRRCFIAGESAGGNIAHHVTVRAAESEFKRVKIVGMILIQPFFGGEERRDSEIRFG 226

Query: 186 RGGYGLTLEKTDSHWKAFLPIGSNRDHPAANVVRSKIPGLNFPLALVVIGGWDPLSDRDK 245
           R GYGLTLE TD  WKA+LP+GSNRDH AANVV S I G+  P ALVVIGG D L DR++
Sbjct: 227 R-GYGLTLEMTDWFWKAWLPVGSNRDHTAANVVGSSISGVKVPAALVVIGGLDLLRDRNR 286

Query: 246 EYIEWLKKSGNEVRVVEYPNAIHGFNWIPDFPEYSMLMQDAKEFVKNIN 293
           EY+EWLKKSG EVRVVEYPN  HGF   PD PEYSML+QDAK+F+  I+
Sbjct: 287 EYVEWLKKSGQEVRVVEYPNGTHGFIGKPDLPEYSMLIQDAKQFINKIS 333

BLAST of Lcy09g000310 vs. ExPASy TrEMBL
Match: A0A6J1JQL4 (probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111487452 PE=4 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 6.5e-115
Identity = 206/290 (71.03%), Postives = 236/290 (81.38%), Query Frame = 0

Query: 6   LRLPHHKLPPS--HGVASFDTVIDSSTPDLWIRVYKPLTFSDADPLPVLIYFHGGGFVSG 65
           LRL   K+PPS   GVASFDT +DSS+ DLW+RVY P T S++D +PV+IYFHGGGFV G
Sbjct: 46  LRLLDRKVPPSTTRGVASFDTTLDSSSSDLWLRVYNPPTCSESDRMPVIIYFHGGGFVFG 105

Query: 66  SPDWPLIDNFSRDFARELGAILVSVNYRLAPEHRFPSQYDDGLHVLKVIDERRISDETLP 125
           S D   ID+FSRDFARE+GAI+VSVNYRLAPEHRFPS YDDG  VLK IDE  I+ ++LP
Sbjct: 106 SADSQQIDSFSRDFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIA-KSLP 165

Query: 126 KNADLSRCFIVGESAGGNIAHHLTVRATTYQFKRLKIVGLIAIQPFFGGEKRTSSELRFG 185
           +NAD+SRCFI GESAGGNI HH+TVRA  Y+FKRL+I G+IAIQPF GGE+RT SE+RFG
Sbjct: 166 ENADISRCFIAGESAGGNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFG 225

Query: 186 RGGYGLTLEKTDSHWKAFLPIGSNRDHPAANVVR--SKIPGLNFPLALVVIGGWDPLSDR 245
           RGG GLTLE  D +WKAFLPIGS+RDHPAANVVR  S + GL  P ALVV GG D LSDR
Sbjct: 226 RGG-GLTLETADWYWKAFLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDR 285

Query: 246 DKEYIEWLKKSGNEVRVVEYPNAIHGFNWIPDFPEYSMLMQDAKEFVKNI 292
            KEY EWL+KSG EVRVVEYPN IHGF+W  D PEYSMLMQDA+EF + +
Sbjct: 286 IKEYCEWLEKSGKEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRV 333

BLAST of Lcy09g000310 vs. ExPASy TrEMBL
Match: A0A5A7T4I6 (Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G00840 PE=4 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 1.3e-110
Identity = 201/291 (69.07%), Postives = 231/291 (79.38%), Query Frame = 0

Query: 6   LRLPHHKLPP--SHGVASFDTVIDSSTPD-LWIRVYKPLTFSDADPLPVLIYFHGGGFVS 65
           L L   K+PP  + GVA+ D  IDSS+ D LWIRVY PLTF ++DPLPV+++FHGGGFV 
Sbjct: 47  LGLLDRKVPPFTTRGVAASDMNIDSSSSDHLWIRVYNPLTFFNSDPLPVIVFFHGGGFVY 106

Query: 66  GSPDWPLIDNFSRDFARELGAILVSVNYRLAPEHRFPSQYDDGLHVLKVIDERRISDETL 125
           GS D P ID F RDFARE+GAI++SVNYRLAPEHRFPSQ+DDG HVLK ++E  IS ETL
Sbjct: 107 GSADSPPIDTFCRDFAREIGAIVISVNYRLAPEHRFPSQFDDGFHVLKAMEEGVIS-ETL 166

Query: 126 PKNADLSRCFIVGESAGGNIAHHLTVRATTYQFKRLKIVGLIAIQPFFGGEKRTSSELRF 185
           P+NADL RCFI GESAGGNIAHH+TVRA   + KRLKIVG+I IQPF GGE+R  SE+RF
Sbjct: 167 PENADLRRCFIAGESAGGNIAHHVTVRAAEGELKRLKIVGMILIQPFLGGEERRDSEIRF 226

Query: 186 GRGGYGLTLEKTDSHWKAFLPIGSNRDHPAANVVRSKIPGLNFPLALVVIGGWDPLSDRD 245
           GR GYG+T+E  D  WKA+LP+GSNRDHPAANVVRS I G+  P A+VVIGG D L DR 
Sbjct: 227 GR-GYGMTVEMADWFWKAWLPVGSNRDHPAANVVRSSISGVKLPPAVVVIGGLDLLRDRQ 286

Query: 246 KEYIEWLKKSGNEVRVVEYPNAIHGFNWIPDFPEYSMLMQDA-KEFVKNIN 293
           KEY+EWLKK G EVRVVEYPN  HGF W PD PEYSMLMQDA K+F  N +
Sbjct: 287 KEYVEWLKKWGEEVRVVEYPNGTHGFIWKPDLPEYSMLMQDAPKQFANNFS 335

BLAST of Lcy09g000310 vs. ExPASy TrEMBL
Match: A0A1S3B2W2 (probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103485400 PE=4 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 1.3e-110
Identity = 201/291 (69.07%), Postives = 231/291 (79.38%), Query Frame = 0

Query: 6   LRLPHHKLPP--SHGVASFDTVIDSSTPD-LWIRVYKPLTFSDADPLPVLIYFHGGGFVS 65
           L L   K+PP  + GVA+ D  IDSS+ D LWIRVY PLTF ++DPLPV+++FHGGGFV 
Sbjct: 47  LGLLDRKVPPFTTRGVAASDMNIDSSSSDHLWIRVYNPLTFFNSDPLPVIVFFHGGGFVY 106

Query: 66  GSPDWPLIDNFSRDFARELGAILVSVNYRLAPEHRFPSQYDDGLHVLKVIDERRISDETL 125
           GS D P ID F RDFARE+GAI++SVNYRLAPEHRFPSQ+DDG HVLK ++E  IS ETL
Sbjct: 107 GSADSPPIDTFCRDFAREIGAIVISVNYRLAPEHRFPSQFDDGFHVLKAMEEGVIS-ETL 166

Query: 126 PKNADLSRCFIVGESAGGNIAHHLTVRATTYQFKRLKIVGLIAIQPFFGGEKRTSSELRF 185
           P+NADL RCFI GESAGGNIAHH+TVRA   + KRLKIVG+I IQPF GGE+R  SE+RF
Sbjct: 167 PENADLRRCFIAGESAGGNIAHHVTVRAAEGELKRLKIVGMILIQPFLGGEERRDSEIRF 226

Query: 186 GRGGYGLTLEKTDSHWKAFLPIGSNRDHPAANVVRSKIPGLNFPLALVVIGGWDPLSDRD 245
           GR GYG+T+E  D  WKA+LP+GSNRDHPAANVVRS I G+  P A+VVIGG D L DR 
Sbjct: 227 GR-GYGMTVEMADWFWKAWLPVGSNRDHPAANVVRSSISGVKLPPAVVVIGGLDLLRDRQ 286

Query: 246 KEYIEWLKKSGNEVRVVEYPNAIHGFNWIPDFPEYSMLMQDA-KEFVKNIN 293
           KEY+EWLKK G EVRVVEYPN  HGF W PD PEYSMLMQDA K+F  N +
Sbjct: 287 KEYVEWLKKWGEEVRVVEYPNGTHGFIWKPDLPEYSMLMQDAPKQFANNFS 335

BLAST of Lcy09g000310 vs. NCBI nr
Match: XP_022964989.1 (probable carboxylesterase 18 [Cucurbita moschata])

HSP 1 Score: 430.3 bits (1105), Expect = 1.4e-116
Identity = 207/289 (71.63%), Postives = 236/289 (81.66%), Query Frame = 0

Query: 6   LRLPHHKLPPS---HGVASFDTVIDSSTPDLWIRVYKPLTFSDADPLPVLIYFHGGGFVS 65
           LRL   K+PPS    GVASFDT +DSS+ DLW+RVY PLT S++D +PV+IYFHGGGFV 
Sbjct: 46  LRLLDRKVPPSSTTRGVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVF 105

Query: 66  GSPDWPLIDNFSRDFARELGAILVSVNYRLAPEHRFPSQYDDGLHVLKVIDERRISDETL 125
           GS D   ID+FSRDFARE+GAI+VSVNYRLAPEHRFPS YDDG  VLK IDE  IS ++L
Sbjct: 106 GSADSQKIDSFSRDFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIS-KSL 165

Query: 126 PKNADLSRCFIVGESAGGNIAHHLTVRATTYQFKRLKIVGLIAIQPFFGGEKRTSSELRF 185
           P+NAD+S CFI GESAGGNI HH+TVRA  Y+FKRLKI G+IAIQPF GGE+RT SE+RF
Sbjct: 166 PENADISSCFIAGESAGGNIVHHVTVRAAEYEFKRLKIAGMIAIQPFLGGEERTKSEIRF 225

Query: 186 GRGGYGLTLEKTDSHWKAFLPIGSNRDHPAANVVRSKIPGLNFPLALVVIGGWDPLSDRD 245
           G+GG GLTLE TD +WKAFLPIGS+RDHP ANVVRS + GL  P ALVV GG D LSDR 
Sbjct: 226 GKGG-GLTLEMTDWYWKAFLPIGSSRDHPGANVVRSDVSGLKLPPALVVNGGLDLLSDRI 285

Query: 246 KEYIEWLKKSGNEVRVVEYPNAIHGFNWIPDFPEYSMLMQDAKEFVKNI 292
           KEY EWL+KSG EVRVVEYPN IHGF+W  D PEYSMLMQDA+EF + +
Sbjct: 286 KEYCEWLEKSGKEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRV 332

BLAST of Lcy09g000310 vs. NCBI nr
Match: KAG6602724.1 (putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033410.1 putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 428.7 bits (1101), Expect = 4.2e-116
Identity = 207/289 (71.63%), Postives = 236/289 (81.66%), Query Frame = 0

Query: 6   LRLPHHKLPPS---HGVASFDTVIDSSTPDLWIRVYKPLTFSDADPLPVLIYFHGGGFVS 65
           LRL   K+PPS    GVASFDT +DSS+ DLW+RVY PLT S++D +PV+IYFHGGGFV 
Sbjct: 46  LRLLDRKVPPSSTTRGVASFDTTLDSSSSDLWLRVYNPLTCSESDRMPVIIYFHGGGFVF 105

Query: 66  GSPDWPLIDNFSRDFARELGAILVSVNYRLAPEHRFPSQYDDGLHVLKVIDERRISDETL 125
           GS D   ID+FSRDFARE+GAI+VSVNY LAPEHRFPS YDDG  VLK IDE  IS ++L
Sbjct: 106 GSADSQQIDSFSRDFAREIGAIVVSVNYCLAPEHRFPSPYDDGFRVLKAIDEGSIS-KSL 165

Query: 126 PKNADLSRCFIVGESAGGNIAHHLTVRATTYQFKRLKIVGLIAIQPFFGGEKRTSSELRF 185
           P+NAD+S CFI GESAGGNI HH+TVRA  Y+FKRL+I G+IAIQPF GGE+RT SE+RF
Sbjct: 166 PENADISSCFIAGESAGGNIVHHVTVRAAEYEFKRLRIAGMIAIQPFLGGEERTKSEIRF 225

Query: 186 GRGGYGLTLEKTDSHWKAFLPIGSNRDHPAANVVRSKIPGLNFPLALVVIGGWDPLSDRD 245
           GRGG GLTLE TD +WKAFLPIGS+RDHPAANVVRS + GL  P ALVV GG D LSDR 
Sbjct: 226 GRGG-GLTLEMTDWYWKAFLPIGSSRDHPAANVVRSDVSGLKLPPALVVNGGLDLLSDRI 285

Query: 246 KEYIEWLKKSGNEVRVVEYPNAIHGFNWIPDFPEYSMLMQDAKEFVKNI 292
           KEY EWL+KSG EVRVVEYPN IHGF+W  D PEYSMLMQDA+EF + +
Sbjct: 286 KEYCEWLEKSGKEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRV 332

BLAST of Lcy09g000310 vs. NCBI nr
Match: XP_004138821.1 (probable carboxylesterase 18 [Cucumis sativus] >KGN63083.1 hypothetical protein Csa_021962 [Cucumis sativus])

HSP 1 Score: 424.1 bits (1089), Expect = 1.0e-114
Identity = 206/289 (71.28%), Postives = 234/289 (80.97%), Query Frame = 0

Query: 6   LRLPHHKLPP--SHGVASFDTVIDSSTPDLWIRVYKPLTFSDADPLPVLIYFHGGGFVSG 65
           LRL   KLPP  S GVA+ D  IDSST DLWIRVY PLTFS++DPLPV+IYFHGGGFV G
Sbjct: 47  LRLFDRKLPPFTSRGVAASDATIDSSTSDLWIRVYNPLTFSNSDPLPVIIYFHGGGFVYG 106

Query: 66  SPDWPLIDNFSRDFARELGAILVSVNYRLAPEHRFPSQYDDGLHVLKVIDERRISDETLP 125
           S D P  D F RDFARE+GAI++SVNYRLAPE RFPSQ+DDG HVLK +D+  IS ET+P
Sbjct: 107 SADAPPTDTFCRDFAREIGAIVISVNYRLAPEDRFPSQFDDGFHVLKAMDKGAIS-ETVP 166

Query: 126 KNADLSRCFIVGESAGGNIAHHLTVRATTYQFKRLKIVGLIAIQPFFGGEKRTSSELRFG 185
           +NADL RCFI GESAGGNIAHH+TVRA   +FKR+KIVG+I IQPFFGGE+R  SE+RFG
Sbjct: 167 ENADLRRCFIAGESAGGNIAHHVTVRAAESEFKRVKIVGMILIQPFFGGEERRDSEIRFG 226

Query: 186 RGGYGLTLEKTDSHWKAFLPIGSNRDHPAANVVRSKIPGLNFPLALVVIGGWDPLSDRDK 245
           R GYGLTLE TD  WKA+LP+GSNRDH AANVV S I G+  P ALVVIGG D L DR++
Sbjct: 227 R-GYGLTLEMTDWFWKAWLPVGSNRDHTAANVVGSSISGVKVPAALVVIGGLDLLRDRNR 286

Query: 246 EYIEWLKKSGNEVRVVEYPNAIHGFNWIPDFPEYSMLMQDAKEFVKNIN 293
           EY+EWLKKSG EVRVVEYPN  HGF   PD PEYSML+QDAK+F+  I+
Sbjct: 287 EYVEWLKKSGQEVRVVEYPNGTHGFIGKPDLPEYSMLIQDAKQFINKIS 333

BLAST of Lcy09g000310 vs. NCBI nr
Match: XP_022990625.1 (probable carboxylesterase 18 [Cucurbita maxima])

HSP 1 Score: 423.7 bits (1088), Expect = 1.3e-114
Identity = 206/290 (71.03%), Postives = 236/290 (81.38%), Query Frame = 0

Query: 6   LRLPHHKLPPS--HGVASFDTVIDSSTPDLWIRVYKPLTFSDADPLPVLIYFHGGGFVSG 65
           LRL   K+PPS   GVASFDT +DSS+ DLW+RVY P T S++D +PV+IYFHGGGFV G
Sbjct: 46  LRLLDRKVPPSTTRGVASFDTTLDSSSSDLWLRVYNPPTCSESDRMPVIIYFHGGGFVFG 105

Query: 66  SPDWPLIDNFSRDFARELGAILVSVNYRLAPEHRFPSQYDDGLHVLKVIDERRISDETLP 125
           S D   ID+FSRDFARE+GAI+VSVNYRLAPEHRFPS YDDG  VLK IDE  I+ ++LP
Sbjct: 106 SADSQQIDSFSRDFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIA-KSLP 165

Query: 126 KNADLSRCFIVGESAGGNIAHHLTVRATTYQFKRLKIVGLIAIQPFFGGEKRTSSELRFG 185
           +NAD+SRCFI GESAGGNI HH+TVRA  Y+FKRL+I G+IAIQPF GGE+RT SE+RFG
Sbjct: 166 ENADISRCFIAGESAGGNIVHHVTVRAAKYEFKRLRIAGMIAIQPFLGGEERTQSEIRFG 225

Query: 186 RGGYGLTLEKTDSHWKAFLPIGSNRDHPAANVVR--SKIPGLNFPLALVVIGGWDPLSDR 245
           RGG GLTLE  D +WKAFLPIGS+RDHPAANVVR  S + GL  P ALVV GG D LSDR
Sbjct: 226 RGG-GLTLETADWYWKAFLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSDR 285

Query: 246 DKEYIEWLKKSGNEVRVVEYPNAIHGFNWIPDFPEYSMLMQDAKEFVKNI 292
            KEY EWL+KSG EVRVVEYPN IHGF+W  D PEYSMLMQDA+EF + +
Sbjct: 286 IKEYCEWLEKSGKEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREFTRRV 333

BLAST of Lcy09g000310 vs. NCBI nr
Match: XP_023549493.1 (probable carboxylesterase 18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 423.3 bits (1087), Expect = 1.8e-114
Identity = 207/289 (71.63%), Postives = 236/289 (81.66%), Query Frame = 0

Query: 6   LRLPHHKLPPS---HGVASFDTVIDSSTPDLWIRVYKPLTFSDADPLPVLIYFHGGGFVS 65
           LRL   K+PPS    GVASFDT +DSS+ DLW+RVY P T S++D +PV+IYFHGGGFV 
Sbjct: 46  LRLLDRKVPPSSTNRGVASFDTTLDSSSSDLWLRVYNPPTCSESDRVPVIIYFHGGGFVF 105

Query: 66  GSPDWPLIDNFSRDFARELGAILVSVNYRLAPEHRFPSQYDDGLHVLKVIDERRISDETL 125
           GS D   ID+FSRDFARE+GAI+VSVNYRLAPEHRFPS YDDG  VLK IDE  IS ++L
Sbjct: 106 GSADSQQIDSFSRDFAREIGAIVVSVNYRLAPEHRFPSPYDDGFRVLKAIDEGSIS-KSL 165

Query: 126 PKNADLSRCFIVGESAGGNIAHHLTVRATTYQFKRLKIVGLIAIQPFFGGEKRTSSELRF 185
           P+NAD+SRCFI GESAGGNI HH+TVRA  Y+FKRL+I G+IAIQPF GGE+RT SE+RF
Sbjct: 166 PENADISRCFIAGESAGGNIVHHVTVRAAEYEFKRLRIAGMIAIQPFLGGEERTKSEIRF 225

Query: 186 GRGGYGLTLEKTDSHWKAFLPIGSNRDHPAANVVR--SKIPGLNFPLALVVIGGWDPLSD 245
           GRGG GLTLE TD +WKAFLPIGS+RDHPAANVVR  S + GL  P ALVV GG D LSD
Sbjct: 226 GRGG-GLTLEVTDWYWKAFLPIGSSRDHPAANVVRSGSDVSGLKLPPALVVNGGLDLLSD 285

Query: 246 RDKEYIEWLKKSGNEVRVVEYPNAIHGFNWIPDFPEYSMLMQDAKEFVK 290
           R KEY EWL++SG EVRVVEYPN IHGF+W  D PEYSMLMQDA+EF +
Sbjct: 286 RIKEYCEWLERSGKEVRVVEYPNGIHGFSWFSDLPEYSMLMQDAREFTR 332

BLAST of Lcy09g000310 vs. TAIR 10
Match: AT5G23530.1 (carboxyesterase 18 )

HSP 1 Score: 256.9 bits (655), Expect = 2.0e-68
Identity = 137/293 (46.76%), Postives = 183/293 (62.46%), Query Frame = 0

Query: 6   LRLPHHKLPPS----HGVASFDTVIDSSTPDLWIRVYKPLTFSDADPLPVLIYFHGGGFV 65
           LRL   + PP+    + V++ D V+D S  DLW R+Y P      D +PV+++FHGGGF 
Sbjct: 43  LRLFDFRAPPNPKPVNIVSTSDFVVDQSR-DLWFRLYTP--HVSGDKIPVVVFFHGGGFA 102

Query: 66  SGSPDWPLIDNFSRDFARELGAILVSVNYRLAPEHRFPSQYDDGLHVLKVIDERRISDET 125
             SP+    DN  R FAR+L A ++SVNYRLAPEHR+P+QYDDG   LK I+E   S   
Sbjct: 103 FLSPNAYPYDNVCRRFARKLPAYVISVNYRLAPEHRYPAQYDDGFDALKYIEENHGS--I 162

Query: 126 LPKNADLSRCFIVGESAGGNIAHHLTV---RATTYQFKRLKIVGLIAIQPFFGGEKRTSS 185
           LP NADLSRCF  G+SAGGNIAH++ +   R     F  +K++GLI+IQPFFGGE+RT +
Sbjct: 163 LPANADLSRCFFAGDSAGGNIAHNVAIRICREPRSSFTAVKLIGLISIQPFFGGEERTEA 222

Query: 186 ELRFGRGGYGLTLEKTDSHWKAFLPIGSNRDHPAANVVRSK---IPGLNFPLALVVIGGW 245
           E +   G   ++ ++TD  WKA   +G NRDH A NV       I GL++P  +VV+ G+
Sbjct: 223 EKQL-VGAPLVSPDRTDWCWKA---MGLNRDHEAVNVGGPNAVDISGLDYPETMVVVAGF 282

Query: 246 DPLSDRDKEYIEWLKKSGNEVRVVEYPNAIHGFNWIPDFPEYSMLMQDAKEFV 289
           DPL D  + Y EWLK  G +  ++EYPN  H F   P+ PE   L+   K+FV
Sbjct: 283 DPLKDWQRSYYEWLKLCGKKATLIEYPNMFHAFYIFPELPEAGQLIMRIKDFV 326

BLAST of Lcy09g000310 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 200.7 bits (509), Expect = 1.7e-51
Identity = 113/289 (39.10%), Postives = 162/289 (56.06%), Query Frame = 0

Query: 15  PSHGVASFDTVIDSSTPDLWIRVYKPLTFS------------DADPLPVLIYFHGGGFVS 74
           P +GV SFD +ID  T +L  RVY+P                D + +PV+++FHGG F  
Sbjct: 59  PVNGVFSFDVIIDRQT-NLLSRVYRPADAGTSPSITDLQNPVDGEIVPVIVFFHGGSFAH 118

Query: 75  GSPDWPLIDNFSRDFARELGAILVSVNYRLAPEHRFPSQYDDGLHVLKVIDERRISDETL 134
            S +  + D   R      GA++VSVNYR APE+R+P  YDDG  VLK ++    S    
Sbjct: 119 SSANSAIYDTLCRRLVGLCGAVVVSVNYRRAPENRYPCAYDDGWAVLKWVNS---SSWLR 178

Query: 135 PKNADLSRCFIVGESAGGNIAHHLTVRATTYQFKRLKIVGLIAIQPFFGGEKRTSSELRF 194
            K     R F+ G+S+GGNI H++ VRA      R+ ++G I + P FGG +RT SE R 
Sbjct: 179 SKKDSKVRIFLAGDSSGGNIVHNVAVRAVE---SRIDVLGNILLNPMFGGTERTESEKRL 238

Query: 195 GRGGYGLTLEKTDSHWKAFLPIGSNRDHPAANVV--RSK-IPGLNFPLALVVIGGWDPLS 254
             G Y +T+   D +W+AFLP G +R+HPA +    RSK + GL+FP +LVV+ G D + 
Sbjct: 239 D-GKYFVTVRDRDWYWRAFLPEGEDREHPACSPFGPRSKSLEGLSFPKSLVVVAGLDLIQ 298

Query: 255 DRDKEYIEWLKKSGNEVRVVEYPNAIHGFNWIPDFPEYSMLMQDAKEFV 289
           D   +Y E LKK+G EV+++    A  GF  +P+   +  +M +   FV
Sbjct: 299 DWQLKYAEGLKKAGQEVKLLYLEQATIGFYLLPNNNHFHTVMDEIAAFV 339

BLAST of Lcy09g000310 vs. TAIR 10
Match: AT3G63010.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 200.3 bits (508), Expect = 2.2e-51
Identity = 109/295 (36.95%), Postives = 165/295 (55.93%), Query Frame = 0

Query: 15  PSHGVASFDTVIDSSTPDLWIRVYKPLT---------------FSDADPLPVLIYFHGGG 74
           P  GV SFD V   ST +L  R+Y+P +                S  + +PVLI+FHGG 
Sbjct: 59  PLDGVFSFDHV--DSTTNLLTRIYQPASLLHQTRHGTLELTKPLSTTEIVPVLIFFHGGS 118

Query: 75  FVSGSPDWPLIDNFSRDFARELGAILVSVNYRLAPEHRFPSQYDDGLHVLKVIDERRISD 134
           F   S +  + D F R      G ++VSV+YR +PEHR+P  YDDG + L  +  R    
Sbjct: 119 FTHSSANSAIYDTFCRRLVTICGVVVVSVDYRRSPEHRYPCAYDDGWNALNWVKSRVWLQ 178

Query: 135 ETLPKNADLSRCFIVGESAGGNIAHHLTVRATTYQFKRLKIVGLIAIQPFFGGEKRTSSE 194
                N  +   ++ G+S+GGNIAH++ VRAT    + +K++G I + P FGG++RT SE
Sbjct: 179 SGKDSNVYV---YLAGDSSGGNIAHNVAVRATN---EGVKVLGNILLHPMFGGQERTQSE 238

Query: 195 LRFGRGGYGLTLEKTDSHWKAFLPIGSNRDHPAANVVRSK---IPGLNFPLALVVIGGWD 254
                G Y +T++  D +W+A+LP G +RDHPA N    +   + G+NFP +LVV+ G D
Sbjct: 239 KTLD-GKYFVTIQDRDWYWRAYLPEGEDRDHPACNPFGPRGQSLKGVNFPKSLVVVAGLD 298

Query: 255 PLSDRDKEYIEWLKKSGNEVRVVEYPNAIHGFNWIPDFPEYSMLMQDAKEFVKNI 292
            + D    Y++ LKK+G EV ++    A  GF ++P+   +  LM++  +FV +I
Sbjct: 299 LVQDWQLAYVDGLKKTGLEVNLLYLKQATIGFYFLPNNDHFHCLMEELNKFVHSI 344

BLAST of Lcy09g000310 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 186.0 bits (471), Expect = 4.4e-47
Identity = 103/273 (37.73%), Postives = 155/273 (56.78%), Query Frame = 0

Query: 32  DLWIRVYKPLTFSDADPLPVLIYFHGGGFVSGSPDWPLIDNFSRDFARELGAILVSVNYR 91
           +L +R+YKP++ S+   LPV+++FHGGGF  GS  WP   NF    A  L A++VS +YR
Sbjct: 59  NLHLRLYKPISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYR 118

Query: 92  LAPEHRFPSQYDDGLHVLKVIDERRISDETL-----PKNADLSRCFIVGESAGGNIAHHL 151
           LAPEHR P+ ++D   VL  + ++ +SD          + D  R F+VG+S+GGNIAH L
Sbjct: 119 LAPEHRLPAAFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQL 178

Query: 152 TVR--ATTYQFKRLKIVGLIAIQPFFGGEKRTSSELRFGRGGYGLTLEKTDSHWKAFLPI 211
            VR  + + +   +++ G + + PFFGGE+RT+SE   G     L+L+  D  W+  LP 
Sbjct: 179 AVRFGSGSIELTPVRVRGYVLMGPFFGGEERTNSE--NGPSEALLSLDLLDKFWRLSLPN 238

Query: 212 GSNRDHPAANVVRSKIP---GLNFPLALVVIGGWDPLSDRDKEYIEWLKK-SGNEVRVVE 271
           G+ RDH  AN      P    ++    LV++GG + L DR KEY   LKK  G  V  +E
Sbjct: 239 GATRDHHMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIE 298

Query: 272 YPNAIHGF-NWIPDFPEYSMLMQDAKEFVKNIN 293
           + N  HGF +  P       +++   +F+ N++
Sbjct: 299 FENKEHGFYSNYPSSEAAEQVLRIIGDFMNNLS 329

BLAST of Lcy09g000310 vs. TAIR 10
Match: AT5G06570.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 186.0 bits (471), Expect = 4.4e-47
Identity = 103/273 (37.73%), Postives = 155/273 (56.78%), Query Frame = 0

Query: 32  DLWIRVYKPLTFSDADPLPVLIYFHGGGFVSGSPDWPLIDNFSRDFARELGAILVSVNYR 91
           +L +R+YKP++ S+   LPV+++FHGGGF  GS  WP   NF    A  L A++VS +YR
Sbjct: 59  NLHLRLYKPISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYR 118

Query: 92  LAPEHRFPSQYDDGLHVLKVIDERRISDETL-----PKNADLSRCFIVGESAGGNIAHHL 151
           LAPEHR P+ ++D   VL  + ++ +SD          + D  R F+VG+S+GGNIAH L
Sbjct: 119 LAPEHRLPAAFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQL 178

Query: 152 TVR--ATTYQFKRLKIVGLIAIQPFFGGEKRTSSELRFGRGGYGLTLEKTDSHWKAFLPI 211
            VR  + + +   +++ G + + PFFGGE+RT+SE   G     L+L+  D  W+  LP 
Sbjct: 179 AVRFGSGSIELTPVRVRGYVLMGPFFGGEERTNSE--NGPSEALLSLDLLDKFWRLSLPN 238

Query: 212 GSNRDHPAANVVRSKIP---GLNFPLALVVIGGWDPLSDRDKEYIEWLKK-SGNEVRVVE 271
           G+ RDH  AN      P    ++    LV++GG + L DR KEY   LKK  G  V  +E
Sbjct: 239 GATRDHHMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIE 298

Query: 272 YPNAIHGF-NWIPDFPEYSMLMQDAKEFVKNIN 293
           + N  HGF +  P       +++   +F+ N++
Sbjct: 299 FENKEHGFYSNYPSSEAAEQVLRIIGDFMNNLS 329

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LT102.8e-6746.76Probable carboxylesterase 18 OS=Arabidopsis thaliana OX=3702 GN=CXE18 PE=1 SV=1[more]
Q940G62.4e-5039.10Gibberellin receptor GID1C OS=Arabidopsis thaliana OX=3702 GN=GID1C PE=1 SV=1[more]
Q9LYC13.2e-5036.95Gibberellin receptor GID1B OS=Arabidopsis thaliana OX=3702 GN=GID1B PE=1 SV=1[more]
Q6L5455.4e-5037.12Gibberellin receptor GID1 OS=Oryza sativa subsp. japonica OX=39947 GN=GID1 PE=1 ... [more]
Q9FG136.2e-4637.73Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HPS26.9e-11771.63probable carboxylesterase 18 OS=Cucurbita moschata OX=3662 GN=LOC111464932 PE=4 ... [more]
A0A0A0LMQ65.0e-11571.28Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G4014... [more]
A0A6J1JQL46.5e-11571.03probable carboxylesterase 18 OS=Cucurbita maxima OX=3661 GN=LOC111487452 PE=4 SV... [more]
A0A5A7T4I61.3e-11069.07Putative carboxylesterase 18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3B2W21.3e-11069.07probable carboxylesterase 18 OS=Cucumis melo OX=3656 GN=LOC103485400 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
XP_022964989.11.4e-11671.63probable carboxylesterase 18 [Cucurbita moschata][more]
KAG6602724.14.2e-11671.63putative carboxylesterase 18, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_004138821.11.0e-11471.28probable carboxylesterase 18 [Cucumis sativus] >KGN63083.1 hypothetical protein ... [more]
XP_022990625.11.3e-11471.03probable carboxylesterase 18 [Cucurbita maxima][more]
XP_023549493.11.8e-11471.63probable carboxylesterase 18 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G23530.12.0e-6846.76carboxyesterase 18 [more]
AT5G27320.11.7e-5139.10alpha/beta-Hydrolases superfamily protein [more]
AT3G63010.12.2e-5136.95alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.14.4e-4737.73alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.24.4e-4737.73alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 52..268
e-value: 1.1E-57
score: 195.3
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 6..297
e-value: 4.6E-79
score: 268.2
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 20..291
NoneNo IPR availablePANTHERPTHR23024:SF434ACETYL ESTERASEcoord: 11..291
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 11..291

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy09g000310.1Lcy09g000310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity