Lcy08g019030 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy08g019030
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	thermospermine synthase ACAULIS5-like
Location	Chr08: 49705615 .. 49708654 (-)
RNA-Seq Expression	Lcy08g019030
Synteny	Lcy08g019030

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCGGAATCCGCCATGCGAAGCAAGGAAAGCTCGCAATCCCCCATTTCCCAATGCAGAGCGTTATGCGTTTACTTCCACTTTCACCATTAACGTAGCTGTCCATCTTCTCTTAGCTTTTCTCGACAAGTTTCTAATTTCCTCGTCACCTGTTTCTACTCTGCGCCTCTCTGTTGCTTTGGCGTCTCCTTCTTCCACTCATTTCGAGTGGGTTCCACATTGATTCCAATTTCCCTCTTATAAATCGCCTCTTCTTGCTTGATTCTTCACTACTACTACTTGTACTCGTTTCCCAAATCTTTCTGCACAATACCCACATTCACTTCAATTTTCTACCATGTTTTCATCCTTCTGCTCTGCCCTTTCTGTTCTCTTTATCCCCAACTCCACTGCTCTCACTCTCGATAATGTCCACCAAGTCTCTGAATGGTTCGAGGAGCAACTCGAGGATGATCTCAAATGGTCCTTTGCAGTCAACAGGTCCATCCTATTTTCTTCATCTTTCTCTTGCTCACAAGGTGTTTGTAAAATGTCTTCCCCTTTTATTGCTCGTGTTTTGTTTGACCGAGTTGTTTGAAATTGAAGCTCATTCTTACAATACTCTATCGCCCTTGCATTGTAGAGTGGATGAAACGAATTTGAGTGTGTTTTAAGTTGAACTGATTCTGACATTCACTTCCTTCAGGATACTTCATGCCGCAACAAGCGAGTTCCAAGAAATAGTTCTCTTGGATACCATGCGATTTGGGAAGGTTTGCTTTTATCATGCATCAGTTTCCTTTCCAATAACAGTTGATTAGTGCAGAGCTGAAAATGAGTTGACTTGACATTCAATTGCAGGCTCTATTGCTTGATGGGAAGCTGCAAAGTGCTGAAAAAGATGAATTCATTTACCATGAGTCCTTGGTTCATCCTGCTCTGCTACTGCATCATAAGTATGCTGTGTTCATATTTGGTTTAGCTCTTTTAACTATTCTTGTTGTGGGTACTGAAGTTGAAATGTGGGACTGCAATCTGAAGCTAAGCAGATTTTGTTCTTATCTTCTCTTTTTGATTTTCTGAATGATTTCAGCCCCAAAACAGTGTTTATTATGGGAGGTGGAGAAGGGTGTACTGCTAGAGAGACACTAAGACACAAAGATATTGAGAAGGTTGTCATGTGCGATATTGATAGGGTATGGTTCTCAAAATTCTAAGATGCACTCATTTGTAAATTCATGTGGCAAAGGATCAATGAAGTTGAGTTATATGACTAAAAAAATTAATGAGATTAGCCATGCTGATTTTGGTATGAAGTCTTTGATTTACATTGTACTGATATCTTTAGAGGGCAACAACATTGTAGGAGGTTGTCAATTTCTGCCGTGAACATCTAACAGAAAACCGAGATGCATTTCAGGACGAGAAGCTTCATATTATTTTTGACGATGCGAAGTGAGTAGCTTTTCCCTTTGGTTTGTAACTTTCTTTTCAATGGTGTATCAAGTTTTAAAGGCACCATTCCATCTGCTATTTGCATTTAGTTATGCAATCTGTGTGTGATGCTCAGCTTGTGAAAACTATATCTCAAAATTTGCTTGAATCTATGCATATTTCCAAAGGATTCAAGTTTTGTAAGTTTAGATTTGAAAGAACTAACCCGATGCAATCTATATATGTTGAAAAATGAAAAAGGGCTGGATTGGAGGGGCGACCAGAGAAGTTCGATGTGATCATTGGCGATTTATCCGATCCACATGAAGGAGGACCATGTAATCATCTGTACACTAAATCCTTCTATGAGGAAGTGATTAAGCCTAAGCTCAATGACAATGGTATCTTTGTTACTCAGGTACATTTTATACATATTCAAAACTCTCGTTATTATTCCTGCTTACTGTTACTAAGATTTCATTACTGTTACTAAAATGCAGGCTGGCCCTGCTGGTATTCTCTCCCACAAGGTCTTCTCATCAATATACAACACCGTAAAGCACGTCTTCAGATGTAAGACATTGCTTAACATTATGCATGCATTGTATCATGTAATTTGCCTGAATTCTTCTTTATAAAGAACGCGTTCAAAATCCTTCCCAGTTAGAGTTCAAACACACGTGAGAGTAGGGATTCAAATCTCTAACTTCTTGGTCGATGATATAATATCTTAATCAATTGAGCTATGCCCACGTTGACATTATTGATGTATCACTAAAGATAAATTCTTGTGCTGATACAAAGCTTATTCGAAGCGGGATATGTCCCATCAATGTTATAAATATAGGAGAGGTCTGTTTGGCCCATAACATAGATTTTGTTGTATCTGCACAGATGTGATCGCATACACAGCTCATGTTCCATCCTATGCAGATTCATGTGGATGGGTTTTGGTAAGCAACTAGAAAGAATTCCTTATCTGTCTTAAAAGAACAGGAGATGAAAAGATATAAATTTGCAATGACAGGCTTGTGATCATCCTCTTAAACTGGACATTGAGGAACTCAACAACAGGATACGTGAGAGAGTTCGAGGCGAGCTACACTACTTGGATGGTGCATTCATCGTATCGTCGACGGTCATAAACAAGACCATTCGTACAGCGTAAGTAGAAAGATAGTTTCTCCATTTCATCTTGATTTGCTTTTATGGCCATTGATTTTTGGATCATGTGTAAATGGAGCGTGTGTTTGTTGTGCGAGCAGGATGATGAATGAGACTCATTTGTTCACTGAGGAAGATGCAAGGTTCGCTCATGGACAAGGGTTGGTTGGCAATGCCTAGAAGCTCTTGGTTCAAACTCACTTCAGACTGTTGCTCAACCAACCAATATATTAGAACCCAGCAGTCAGCAGCATTTGCATCTAAAATTATAACTACATGTTTACTTGAGTGAAGCTGTATCTCGTTAGTTTCAAGCTATTGTGTAATGTTTGACACTTGTTTTATTGCATTTTAAATGTTGAAATTATTATTATTTTTTCGATCTGTGGGAGATTCAAGCCTCATACCTCTTGATCAATGATACATATTTTAGGCAAGTTGAACTATGCTCATGTTGAAATGAAAT

mRNA sequence

TCGGAATCCGCCATGCGAAGCAAGGAAAGCTCGCAATCCCCCATTTCCCAATGCAGAGCGTTATGCGTTTACTTCCACTTTCACCATTAACGTAGCTGTCCATCTTCTCTTAGCTTTTCTCGACAAGTTTCTAATTTCCTCGTCACCTGTTTCTACTCTGCGCCTCTCTGTTGCTTTGGCGTCTCCTTCTTCCACTCATTTCGAGTGGGTTCCACATTGATTCCAATTTCCCTCTTATAAATCGCCTCTTCTTGCTTGATTCTTCACTACTACTACTTGTACTCGTTTCCCAAATCTTTCTGCACAATACCCACATTCACTTCAATTTTCTACCATGTTTTCATCCTTCTGCTCTGCCCTTTCTGTTCTCTTTATCCCCAACTCCACTGCTCTCACTCTCGATAATGTCCACCAAGTCTCTGAATGGTTCGAGGAGCAACTCGAGGATGATCTCAAATGGTCCTTTGCAGTCAACAGGATACTTCATGCCGCAACAAGCGAGTTCCAAGAAATAGTTCTCTTGGATACCATGCGATTTGGGAAGGCTCTATTGCTTGATGGGAAGCTGCAAAGTGCTGAAAAAGATGAATTCATTTACCATGAGTCCTTGGTTCATCCTGCTCTGCTACTGCATCATAACCCCAAAACAGTGTTTATTATGGGAGGTGGAGAAGGGTGTACTGCTAGAGAGACACTAAGACACAAAGATATTGAGAAGGTTGTCATGTGCGATATTGATAGGGAGGTTGTCAATTTCTGCCGTGAACATCTAACAGAAAACCGAGATGCATTTCAGGACGAGAAGCTTCATATTATTTTTGACGATGCGAAGGCTGGATTGGAGGGGCGACCAGAGAAGTTCGATGTGATCATTGGCGATTTATCCGATCCACATGAAGGAGGACCATGTAATCATCTGTACACTAAATCCTTCTATGAGGAAGTGATTAAGCCTAAGCTCAATGACAATGGTATCTTTGTTACTCAGGCTGGCCCTGCTGGTATTCTCTCCCACAAGGTCTTCTCATCAATATACAACACCGTAAAGCACGTCTTCAGATATGTGATCGCATACACAGCTCATGTTCCATCCTATGCAGATTCATGTGGATGGGTTTTGGCTTGTGATCATCCTCTTAAACTGGACATTGAGGAACTCAACAACAGGATACGTGAGAGAGTTCGAGGCGAGCTACACTACTTGGATGGTGCATTCATCGTATCGTCGACGGTCATAAACAAGACCATTCGTACAGCGATGATGAATGAGACTCATTTGTTCACTGAGGAAGATGCAAGGTTCGCTCATGGACAAGGGTTGGTTGGCAATGCCTAGAAGCTCTTGGTTCAAACTCACTTCAGACTGTTGCTCAACCAACCAATATATTAGAACCCAGCAGTCAGCAGCATTTGCATCTAAAATTATAACTACATGTTTACTTGAGTGAAGCTGTATCTCGTTAGTTTCAAGCTATTGTGTAATGTTTGACACTTGTTTTATTGCATTTTAAATGTTGAAATTATTATTATTTTTTCGATCTGTGGGAGATTCAAGCCTCATACCTCTTGATCAATGATACATATTTTAGGCAAGTTGAACTATGCTCATGTTGAAATGAAAT

Coding sequence (CDS)

ATGTTTTCATCCTTCTGCTCTGCCCTTTCTGTTCTCTTTATCCCCAACTCCACTGCTCTCACTCTCGATAATGTCCACCAAGTCTCTGAATGGTTCGAGGAGCAACTCGAGGATGATCTCAAATGGTCCTTTGCAGTCAACAGGATACTTCATGCCGCAACAAGCGAGTTCCAAGAAATAGTTCTCTTGGATACCATGCGATTTGGGAAGGCTCTATTGCTTGATGGGAAGCTGCAAAGTGCTGAAAAAGATGAATTCATTTACCATGAGTCCTTGGTTCATCCTGCTCTGCTACTGCATCATAACCCCAAAACAGTGTTTATTATGGGAGGTGGAGAAGGGTGTACTGCTAGAGAGACACTAAGACACAAAGATATTGAGAAGGTTGTCATGTGCGATATTGATAGGGAGGTTGTCAATTTCTGCCGTGAACATCTAACAGAAAACCGAGATGCATTTCAGGACGAGAAGCTTCATATTATTTTTGACGATGCGAAGGCTGGATTGGAGGGGCGACCAGAGAAGTTCGATGTGATCATTGGCGATTTATCCGATCCACATGAAGGAGGACCATGTAATCATCTGTACACTAAATCCTTCTATGAGGAAGTGATTAAGCCTAAGCTCAATGACAATGGTATCTTTGTTACTCAGGCTGGCCCTGCTGGTATTCTCTCCCACAAGGTCTTCTCATCAATATACAACACCGTAAAGCACGTCTTCAGATATGTGATCGCATACACAGCTCATGTTCCATCCTATGCAGATTCATGTGGATGGGTTTTGGCTTGTGATCATCCTCTTAAACTGGACATTGAGGAACTCAACAACAGGATACGTGAGAGAGTTCGAGGCGAGCTACACTACTTGGATGGTGCATTCATCGTATCGTCGACGGTCATAAACAAGACCATTCGTACAGCGATGATGAATGAGACTCATTTGTTCACTGAGGAAGATGCAAGGTTCGCTCATGGACAAGGGTTGGTTGGCAATGCCTAG

Protein sequence

MFSSFCSALSVLFIPNSTALTLDNVHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQEIVLLDTMRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARETLRHKDIEKVVMCDIDREVVNFCREHLTENRDAFQDEKLHIIFDDAKAGLEGRPEKFDVIIGDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACDHPLKLDIEELNNRIRERVRGELHYLDGAFIVSSTVINKTIRTAMMNETHLFTEEDARFAHGQGLVGNA

Homology

[Close]

BLAST of Lcy08g019030 vs. ExPASy Swiss-Prot
Match: Q9S7X6 (Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 6.0e-119
Identity = 199/314 (63.38%), Postives = 248/314 (78.98%), Query Frame = 0


Query: 17  STALTLDNVHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQEIVLLDTMRFGKALLLDG 76
           S   TL +  Q   W+EE ++DDLKWSFA+N +LH  TSE+Q+I LLDT RFGK L++DG
Sbjct: 21  SPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDG 80

Query: 77  KLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARETLRHKDIEKVVMCDIDR 136
           K+QSAE+DEFIYHE L+HPALL H NPKTVFIMGGGEG  ARE L+H  IEKVVMCDID+
Sbjct: 81  KMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQ 140

Query: 137 EVVNFCREHLTENRDAFQDEKLHIIFDDAKAGLEGRPEKFDVIIGDLSDPHEGGPCNHLY 196
           EVV+FCR  LT N DAF ++KL ++  DAKA LE R EKFD+I+GDL+DP EGGPC  LY
Sbjct: 141 EVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLY 200

Query: 197 TKSFYEEVIKPKLNDNGIFVTQAGPAGILSHK-VFSSIYNTVKHVFRYVIAYTAHVPSYA 256
           TKSFY+ ++KPKL+ NGIFVTQAGPAGI +HK VF+SIYNT+K VF+YV AYTAHVPS+A
Sbjct: 201 TKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFA 260

Query: 257 DSCGWVLACDHPLKLDIEELNNRIRERVRGELHYLDGAFIVSSTVINKTIRTAMMNETHL 316
           D+ GWV+A DH   ++++E++ RI ERV GEL YL+    VS+  +NKTI  A+  ET +
Sbjct: 261 DTWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALEKETEV 320

Query: 317 FTEEDARFAHGQGL 330
           ++EE+ARF HG G+
Sbjct: 321 YSEENARFIHGHGV 334

[Close]

BLAST of Lcy08g019030 vs. ExPASy Swiss-Prot
Match: Q975S5 (Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=speE PE=3 SV=1)

HSP 1 Score: 223.8 bits (569), Expect = 2.9e-57
Identity = 116/279 (41.58%), Postives = 173/279 (62.01%), Query Frame = 0


Query: 45  AVNRILHAATSEFQEIVLLDTMRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPK 104
           A+ +++    + +Q  +L++  RFGKAL+LDGK+QS   DEFIYHE+LVHP LL  +NP+
Sbjct: 20  AITKVIAEEKTPYQRALLVELARFGKALILDGKIQSTITDEFIYHEALVHPLLLSINNPE 79

Query: 105 TVFIMGGGEGCTARETLRHKDIEKVVMCDIDREVVNFCREHLTE-NRDAFQDEKLHIIFD 164
            + I+GGGEG T RE L+HK I+ V M DID  V++F +++L E ++ AF + K  ++ +
Sbjct: 80  KILILGGGEGATLREVLKHKTIKNVTMVDIDPVVIDFAKKYLQEWHQGAFDNPKSKLVIE 139

Query: 165 DAKAGLEGRPEKFDVIIGDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAG 224
           D    ++   EKFD ++ DL+DP +  P   LYTK FYEEV   +++  GI      P+ 
Sbjct: 140 DGYKFIKETKEKFDAVVIDLTDPIKDSPSQMLYTKEFYEEV--KRISKWGIVTQATSPS- 199

Query: 225 ILSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACD--HPLKLDIEELNNRIRE 284
             S + FS IYNT+KHVF+ V A   +VP++    G+V A D  +P +   EE+NNRI+E
Sbjct: 200 -FSLETFSIIYNTIKHVFKKVSAGITYVPAFDGLWGFVYASDEVNPAEFSKEEINNRIKE 259

Query: 285 RVRGELHYLDGAFIVSSTVINKTIRTAMMNETHLFTEED 321
           R+ G L + DG   ++   I K IR  +  E  + T E+
Sbjct: 260 RIDGSLRFYDGETHITMFSIPKHIREVLEKENKISTREN 294

[Close]

BLAST of Lcy08g019030 vs. ExPASy Swiss-Prot
Match: Q9YE02 (Polyamine aminopropyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=speE PE=3 SV=2)

HSP 1 Score: 214.5 bits (545), Expect = 1.8e-54
Identity = 122/299 (40.80%), Postives = 174/299 (58.19%), Query Frame = 0


Query: 31  WFEEQLEDDLKWSFAVNRILHAATSEFQEIVLLDTMRFGKALLLDGKLQSAEKDEFIYHE 90
           WF E L  D      +  ++    S FQEI ++     GK L+LDGK QS+E DEF+YHE
Sbjct: 15  WFLEWLTPDRATLKHIEDVIFQGRSRFQEIAVVRVSGEGKVLVLDGKTQSSESDEFMYHE 74

Query: 91  SLVHPALLLHHNPKTVFIMGGGEGCTARETLRHKDIEKVVMCDIDREVVNFCREHLTE-N 150
           +LVHPA++LH +P+ V I+GGGEG T RE L+H+ +EK VM DID  VVN  REHL E +
Sbjct: 75  ALVHPAMILHGSPRKVLILGGGEGATLREVLKHRSVEKAVMVDIDETVVNVAREHLREWH 134

Query: 151 RDAFQDEKLHIIFDDAKAGLEGRPEK-FDVIIGDLSDPHEGGPCNHLYTKSFYEEVIKPK 210
           R AF D +  ++ DDA   +  + E  FDV+I DL DP E GP   LY++ +Y  ++K  
Sbjct: 135 RGAFDDPRAEVVIDDAWNYVASKAETGFDVVIADLVDPLEAGPATRLYSEEYY-RMVKDV 194

Query: 211 LNDNGIFVTQAGPAGILSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACDHP- 270
           +N  G+FVTQA     L+ +  + I NTV  VF++V +Y  +VPS+    G+V+A D   
Sbjct: 195 MNPGGVFVTQAVSISHLT-EYHAIIRNTVARVFKHVESYGVYVPSFDSMWGFVVASDDKD 254

Query: 271 --LKLDIEELNNRIRERVRG-ELHYLDGAFIVSSTVINKTIRTAMMNETHLFTEEDARF 324
             +  D      R+  +++G EL +LD A ++    I K  R A+  E    T ++  F
Sbjct: 255 PRILGDRGFFETRLSHQLQGAELRFLDYASMLHMLNIPKMYREAIAREKRYATLDNQVF 311

[Close]

BLAST of Lcy08g019030 vs. ExPASy Swiss-Prot
Match: Q4JAZ8 (Polyamine aminopropyltransferase OS=Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) OX=330779 GN=speE PE=3 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 1.1e-53
Identity = 116/279 (41.58%), Postives = 168/279 (60.22%), Query Frame = 0


Query: 45  AVNRILHAATSEFQEIVLLDTMRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPK 104
           A+ RIL    SEFQ ++L +  RFGKAL++DGK+QS   DEFIYHESLVHP L+   +P+
Sbjct: 20  AIKRILIEERSEFQRVILAELFRFGKALIIDGKIQSTLADEFIYHESLVHPLLISLEDPE 79

Query: 105 TVFIMGGGEGCTARETLRHKDIEKVVMCDIDREVVNFCREHLTE-NRDAFQDEKLHIIFD 164
            + I+GGGEG T RE LR+K + KV M DID  V+ F +++L E +  +F D + ++I  
Sbjct: 80  NILILGGGEGATLREALRYKSVRKVTMVDIDPVVIKFAKQNLQEWHMGSFDDSRTNLIIG 139

Query: 165 DAKAGLEGRPEKFDVIIGDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAG 224
           D    ++   EK++ II DL+DP +  P   LYT  FY + +K  +  NG  VTQA  + 
Sbjct: 140 DGYKFVKETNEKYNAIILDLTDPIQDSPSQLLYTSEFYRD-LKSIITPNGGLVTQA-TSP 199

Query: 225 ILSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACD--HPLKLDIEELNNRIRE 284
             S   F+ IY+T+K VF+ V A   +VPS+    G++ A D  +P  L+  ++N+RI+E
Sbjct: 200 SFSLDTFAIIYSTLKTVFKNVSAGITYVPSFDGLWGFIYASDVTNPAHLNRNQINDRIKE 259

Query: 285 RVRGELHYLDGAFIVSSTVINKTIRTAMMNETHLFTEED 321
           RV G L + DG        + K IR  + NE  + T E+
Sbjct: 260 RVTGSLRFYDGETHEMLFRVPKYIREKIENEKRVSTREN 296

[Close]

BLAST of Lcy08g019030 vs. ExPASy Swiss-Prot
Match: Q72K55 (Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) OX=262724 GN=speE PE=3 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 2.6e-53
Identity = 112/287 (39.02%), Postives = 170/287 (59.23%), Query Frame = 0


Query: 46  VNRILHAATSEFQEIVLLDTMRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKT 105
           + R++ +  + FQ+  L ++  FGK L+LD  +QS E+DE+IYHE+LVHPA+L H  PK 
Sbjct: 21  MERVIASGKTPFQDYFLFESKGFGKVLILDKDVQSTERDEYIYHETLVHPAMLTHPEPKR 80

Query: 106 VFIMGGGEGCTARETLRHKDIEKVVMCDIDREVVNFCREHLTE-NRDAFQDEKLHIIFDD 165
           V I+GGGEG T RE L+H  +EK VM DID E+V   + H+ E ++ AF D +  ++ DD
Sbjct: 81  VLIVGGGEGATLREVLKHPTVEKAVMVDIDGELVEVAKRHMPEWHQGAFDDPRAVLVIDD 140

Query: 166 AKAGLEGRPEKFDVIIGDLSDP-HEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAG 225
           A+A LE   E++DV+I DL+DP  E  P   LYT  FY  ++K  LN  G+   QAG   
Sbjct: 141 ARAYLERTEERYDVVIIDLTDPVGEDNPARLLYTVEFY-RLVKAHLNPGGVMGMQAGMIL 200

Query: 226 ILSHKVFSSIYNTVKHVFRYVIAYTAHVPSYADSCGWVLACD--HPLKLDIEELNNRIRE 285
           +  H+V   ++ TV+  FRYV +Y  H+P +  + G++LA D   P       +  RIRE
Sbjct: 201 LTHHRVHPVVHRTVREAFRYVRSYKNHIPGFFLNFGFLLASDAFDPAAFSEGVIEARIRE 260

Query: 286 RVRGELHYLDGAFIVSSTVINKTIRTAMMNETHLFTEEDARFAHGQG 329
           R    L +L   ++ +  V+ K +  A+  ET + T+++  +   +G
Sbjct: 261 R-NLALRHLTAPYLEAMFVLPKDLLEALEKETMVSTDQNPFYVTPEG 305

[Close]

BLAST of Lcy08g019030 vs. ExPASy TrEMBL
Match: A0A0A0LET5 (PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000240 PE=3 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 7.0e-179
Identity = 300/333 (90.09%), Postives = 322/333 (96.70%), Query Frame = 0


Query: 1   MFSSFCSALSVLFIPNSTALTLDNVHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQEI 60
           MFSSFCSALSVLF+PNS++L LD++ Q+ +WFEE+LEDDLKWSFAVNR+LHA TSEFQ+I
Sbjct: 1   MFSSFCSALSVLFVPNSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTMRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDT RFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120

Query: 121 LRHKDIEKVVMCDIDREVVNFCREHLTENRDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180
           L+HK IEKVVMCDIDR+VVNFCR HL EN+DAFQDE+LHIIFDDAKAGLEGRPEKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYE+VIKPKL+DNGIFVTQAGPAGILSHKVFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPLKLDIEELNNRIRERVRGELHYLDGAFIVSSTV 300
           FRYVIAYTAHVPSYADSCGWVLA DHP+KLDIE+LNN+IRERV+GELHYLDGAFIVSSTV
Sbjct: 241 FRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTV 300

Query: 301 INKTIRTAMMNETHLFTEEDARFAHGQGLVGNA 334
           INKTIRT MMNETH+FTEEDARFAHG+GLV NA
Sbjct: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVANA 333

[Close]

BLAST of Lcy08g019030 vs. ExPASy TrEMBL
Match: A0A5A7UZV1 (Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G001170 PE=3 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 2.5e-176
Identity = 298/333 (89.49%), Postives = 319/333 (95.80%), Query Frame = 0


Query: 1   MFSSFCSALSVLFIPNSTALTLDNVHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQEI 60
           MFSSFCSALSVLFIPNS++L LD++HQ+ +WFEE+LEDDLKWSFAVNR+LHA TSEFQ+I
Sbjct: 1   MFSSFCSALSVLFIPNSSSLPLDHIHQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTMRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDT RFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120

Query: 121 LRHKDIEKVVMCDIDREVVNFCREHLTENRDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180
           L+HK IEKVVMCDIDR+VVNFCR HL EN+DAFQDEKLHIIFDDAKAGLEGR EKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRAEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYE+VIKPKL+DNGIFVTQAGPAGILSHKVFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPLKLDIEELNNRIRERVRGELHYLDGAFIVSSTV 300
           F+YVIAYTAHVPSYADSCGWVLA DHP++LD E+LNN+I ERVRGEL YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHVPSYADSCGWVLASDHPIRLDKEDLNNKIHERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRTAMMNETHLFTEEDARFAHGQGLVGNA 334
           INKTIRT MMNETH+FTEEDARFAHG+GLVGNA
Sbjct: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVGNA 333

[Close]

BLAST of Lcy08g019030 vs. ExPASy TrEMBL
Match: A0A1S3CAR3 (thermospermine synthase ACAULIS5-like OS=Cucumis melo OX=3656 GN=LOC103498720 PE=3 SV=1)

HSP 1 Score: 627.9 bits (1618), Expect = 2.5e-176
Identity = 298/333 (89.49%), Postives = 319/333 (95.80%), Query Frame = 0


Query: 1   MFSSFCSALSVLFIPNSTALTLDNVHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQEI 60
           MFSSFCSALSVLFIPNS++L LD++HQ+ +WFEE+LEDDLKWSFAVNR+LHA TSEFQ+I
Sbjct: 1   MFSSFCSALSVLFIPNSSSLPLDHIHQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTMRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDT RFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120

Query: 121 LRHKDIEKVVMCDIDREVVNFCREHLTENRDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180
           L+HK IEKVVMCDIDR+VVNFCR HL EN+DAFQDEKLHIIFDDAKAGLEGR EKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRAEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYE+VIKPKL+DNGIFVTQAGPAGILSHKVFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPLKLDIEELNNRIRERVRGELHYLDGAFIVSSTV 300
           F+YVIAYTAHVPSYADSCGWVLA DHP++LD E+LNN+I ERVRGEL YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHVPSYADSCGWVLASDHPIRLDKEDLNNKIHERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRTAMMNETHLFTEEDARFAHGQGLVGNA 334
           INKTIRT MMNETH+FTEEDARFAHG+GLVGNA
Sbjct: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVGNA 333

[Close]

BLAST of Lcy08g019030 vs. ExPASy TrEMBL
Match: A0A6J1G4J8 (thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111450716 PE=3 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.4e-163
Identity = 283/333 (84.98%), Postives = 302/333 (90.69%), Query Frame = 0


Query: 1   MFSSFCSALSVLFIPNSTALTLDNVHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQEI 60
           M S  CSAL  L IP S +     V  VSEWFEEQLEDDLKWSFAVNRILHAATS+FQ+I
Sbjct: 1   MLSPLCSALPALSIPTSNSTA---VPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDI 60

Query: 61  VLLDTMRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDT RFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHHNPKTVFI+GGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARET 120

Query: 121 LRHKDIEKVVMCDIDREVVNFCREHLTENRDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180
           L+HK IEKVVMCDIDR+VVNFCR HL+EN+DAFQDEKLHIIFDDAK GLEG+PEKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
            DL+DPHEGGPCNHLYTKSFYEE IKPKLNDNGIFVTQAGP+GILS+ VFSSIYNTVKHV
Sbjct: 181 SDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPLKLDIEELNNRIRERVRGELHYLDGAFIVSSTV 300
           F+YVIAYTAH+PSYADS GWVLA DHP KLDIE+L+ RIRERVRGEL YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHIPSYADSSGWVLASDHPFKLDIEDLDKRIRERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRTAMMNETHLFTEEDARFAHGQGLVGNA 334
           INKTIRTAMMNETH+FTEEDARF HGQGL  +A
Sbjct: 301 INKTIRTAMMNETHVFTEEDARFTHGQGLASHA 330

[Close]

BLAST of Lcy08g019030 vs. ExPASy TrEMBL
Match: A0A6J1KJ05 (thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC111493662 PE=3 SV=1)

HSP 1 Score: 580.5 bits (1495), Expect = 4.5e-162
Identity = 280/333 (84.08%), Postives = 302/333 (90.69%), Query Frame = 0


Query: 1   MFSSFCSALSVLFIPNSTALTLDNVHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQEI 60
           M S  CSAL  L IP S +     V QVSEWFEEQLEDDLKWSFAV RILHAATS+FQ+I
Sbjct: 1   MLSPLCSALPALSIPTSNSTA---VPQVSEWFEEQLEDDLKWSFAVTRILHAATSKFQDI 60

Query: 61  VLLDTMRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDT RFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHHNPKTVFI+GGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARET 120

Query: 121 LRHKDIEKVVMCDIDREVVNFCREHLTENRDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180
           L+HK IEKVVMCDIDR+VVNFCR HL+EN+DAFQDEKLHIIF+DAK GLEG+PEKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFNDAKVGLEGQPEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
            DL+DPHEGGPCNHLYTKSFYEE IKPKLNDNGIFVTQAGP+GILS+ VFSSIYNTVKHV
Sbjct: 181 SDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPLKLDIEELNNRIRERVRGELHYLDGAFIVSSTV 300
           F+YVIAYTAH+PSYADS GWVLA DHP +LD+E+L+ RIRERVRGEL YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHIPSYADSSGWVLASDHPFELDMEDLDKRIRERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRTAMMNETHLFTEEDARFAHGQGLVGNA 334
           INKTIRTAMMNETH+FTEEDARF HGQGL  +A
Sbjct: 301 INKTIRTAMMNETHVFTEEDARFTHGQGLASHA 330

[Close]

BLAST of Lcy08g019030 vs. NCBI nr
Match: XP_038890496.1 (thermospermine synthase ACAULIS5-like [Benincasa hispida])

HSP 1 Score: 649.0 bits (1673), Expect = 2.1e-182
Identity = 308/333 (92.49%), Postives = 326/333 (97.90%), Query Frame = 0


Query: 1   MFSSFCSALSVLFIPNSTALTLDNVHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQEI 60
           MFSSFCSALSVLFIPNST+L LD++HQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQ+I
Sbjct: 1   MFSSFCSALSVLFIPNSTSLPLDHIHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQDI 60

Query: 61  VLLDTMRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDT RFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120

Query: 121 LRHKDIEKVVMCDIDREVVNFCREHLTENRDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180
           L+HKDIEKVVMCDIDR+VVNFCR HL EN+DAFQDEKLHIIFDDAKAGLEGRPEKFDVII
Sbjct: 121 LKHKDIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSH+VFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHEVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPLKLDIEELNNRIRERVRGELHYLDGAFIVSSTV 300
           F+YVIAYTAHVPSYADSCGWVLACDHP++LD+E+LNNRIRERVRGELHYLDGA+IVSSTV
Sbjct: 241 FKYVIAYTAHVPSYADSCGWVLACDHPIELDMEDLNNRIRERVRGELHYLDGAYIVSSTV 300

Query: 301 INKTIRTAMMNETHLFTEEDARFAHGQGLVGNA 334
           +NKTIR +MMNETHLFTEEDARFAHG+GLV +A
Sbjct: 301 VNKTIRASMMNETHLFTEEDARFAHGRGLVSDA 333

[Close]

BLAST of Lcy08g019030 vs. NCBI nr
Match: XP_004141174.1 (thermospermine synthase ACAULIS5 [Cucumis sativus] >KGN60515.1 hypothetical protein Csa_019446 [Cucumis sativus])

HSP 1 Score: 636.3 bits (1640), Expect = 1.4e-178
Identity = 300/333 (90.09%), Postives = 322/333 (96.70%), Query Frame = 0


Query: 1   MFSSFCSALSVLFIPNSTALTLDNVHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQEI 60
           MFSSFCSALSVLF+PNS++L LD++ Q+ +WFEE+LEDDLKWSFAVNR+LHA TSEFQ+I
Sbjct: 1   MFSSFCSALSVLFVPNSSSLPLDHIQQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTMRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDT RFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120

Query: 121 LRHKDIEKVVMCDIDREVVNFCREHLTENRDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180
           L+HK IEKVVMCDIDR+VVNFCR HL EN+DAFQDE+LHIIFDDAKAGLEGRPEKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDERLHIIFDDAKAGLEGRPEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYE+VIKPKL+DNGIFVTQAGPAGILSHKVFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPLKLDIEELNNRIRERVRGELHYLDGAFIVSSTV 300
           FRYVIAYTAHVPSYADSCGWVLA DHP+KLDIE+LNN+IRERV+GELHYLDGAFIVSSTV
Sbjct: 241 FRYVIAYTAHVPSYADSCGWVLASDHPIKLDIEDLNNKIRERVQGELHYLDGAFIVSSTV 300

Query: 301 INKTIRTAMMNETHLFTEEDARFAHGQGLVGNA 334
           INKTIRT MMNETH+FTEEDARFAHG+GLV NA
Sbjct: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVANA 333

[Close]

BLAST of Lcy08g019030 vs. NCBI nr
Match: XP_008459675.1 (PREDICTED: thermospermine synthase ACAULIS5-like [Cucumis melo] >KAA0061473.1 thermospermine synthase ACAULIS5-like [Cucumis melo var. makuwa] >TYK10801.1 thermospermine synthase ACAULIS5-like [Cucumis melo var. makuwa])

HSP 1 Score: 627.9 bits (1618), Expect = 5.1e-176
Identity = 298/333 (89.49%), Postives = 319/333 (95.80%), Query Frame = 0


Query: 1   MFSSFCSALSVLFIPNSTALTLDNVHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQEI 60
           MFSSFCSALSVLFIPNS++L LD++HQ+ +WFEE+LEDDLKWSFAVNR+LHA TSEFQ+I
Sbjct: 1   MFSSFCSALSVLFIPNSSSLPLDHIHQLPQWFEEELEDDLKWSFAVNRVLHATTSEFQDI 60

Query: 61  VLLDTMRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDT RFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGSTARET 120

Query: 121 LRHKDIEKVVMCDIDREVVNFCREHLTENRDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180
           L+HK IEKVVMCDIDR+VVNFCR HL EN+DAFQDEKLHIIFDDAKAGLEGR EKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLKENQDAFQDEKLHIIFDDAKAGLEGRAEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
           GDLSDPHEGGPCNHLYTKSFYE+VIKPKL+DNGIFVTQAGPAGILSHKVFSSIYNTVKHV
Sbjct: 181 GDLSDPHEGGPCNHLYTKSFYEDVIKPKLSDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPLKLDIEELNNRIRERVRGELHYLDGAFIVSSTV 300
           F+YVIAYTAHVPSYADSCGWVLA DHP++LD E+LNN+I ERVRGEL YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHVPSYADSCGWVLASDHPIRLDKEDLNNKIHERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRTAMMNETHLFTEEDARFAHGQGLVGNA 334
           INKTIRT MMNETH+FTEEDARFAHG+GLVGNA
Sbjct: 301 INKTIRTLMMNETHVFTEEDARFAHGRGLVGNA 333

[Close]

BLAST of Lcy08g019030 vs. NCBI nr
Match: XP_022946737.1 (thermospermine synthase ACAULIS5-like [Cucurbita moschata])

HSP 1 Score: 585.5 bits (1508), Expect = 2.9e-163
Identity = 283/333 (84.98%), Postives = 302/333 (90.69%), Query Frame = 0


Query: 1   MFSSFCSALSVLFIPNSTALTLDNVHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQEI 60
           M S  CSAL  L IP S +     V  VSEWFEEQLEDDLKWSFAVNRILHAATS+FQ+I
Sbjct: 1   MLSPLCSALPALSIPTSNSTA---VPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDI 60

Query: 61  VLLDTMRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDT RFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHHNPKTVFI+GGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARET 120

Query: 121 LRHKDIEKVVMCDIDREVVNFCREHLTENRDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180
           L+HK IEKVVMCDIDR+VVNFCR HL+EN+DAFQDEKLHIIFDDAK GLEG+PEKFDVII
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDVII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
            DL+DPHEGGPCNHLYTKSFYEE IKPKLNDNGIFVTQAGP+GILS+ VFSSIYNTVKHV
Sbjct: 181 SDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPLKLDIEELNNRIRERVRGELHYLDGAFIVSSTV 300
           F+YVIAYTAH+PSYADS GWVLA DHP KLDIE+L+ RIRERVRGEL YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHIPSYADSSGWVLASDHPFKLDIEDLDKRIRERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRTAMMNETHLFTEEDARFAHGQGLVGNA 334
           INKTIRTAMMNETH+FTEEDARF HGQGL  +A
Sbjct: 301 INKTIRTAMMNETHVFTEEDARFTHGQGLASHA 330

[Close]

BLAST of Lcy08g019030 vs. NCBI nr
Match: KAG7030499.1 (Thermospermine synthase ACAULIS5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 584.7 bits (1506), Expect = 5.0e-163
Identity = 281/333 (84.38%), Postives = 302/333 (90.69%), Query Frame = 0


Query: 1   MFSSFCSALSVLFIPNSTALTLDNVHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQEI 60
           M S  CSAL  L IP S +     V  VSEWFEEQLEDDLKWSFAVNRILHAATS+FQ+I
Sbjct: 1   MLSPLCSALPALSIPTSNSTA---VPHVSEWFEEQLEDDLKWSFAVNRILHAATSKFQDI 60

Query: 61  VLLDTMRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARET 120
           VLLDT RFGKALLLDGKLQSAEKDEFIYHES+VHPALLLHHNPKTVFI+GGGEG TARET
Sbjct: 61  VLLDTKRFGKALLLDGKLQSAEKDEFIYHESMVHPALLLHHNPKTVFILGGGEGSTARET 120

Query: 121 LRHKDIEKVVMCDIDREVVNFCREHLTENRDAFQDEKLHIIFDDAKAGLEGRPEKFDVII 180
           L+HK IEKVVMCDIDR+VVNFCR HL+EN+DAFQDEKLHIIFDDAK GLEG+PEKFD+II
Sbjct: 121 LKHKSIEKVVMCDIDRDVVNFCRAHLSENQDAFQDEKLHIIFDDAKVGLEGQPEKFDIII 180

Query: 181 GDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGPAGILSHKVFSSIYNTVKHV 240
            DL+DPHEGGPCNHLYTKSFYEE IKPKLNDNGIFVTQAGP+GILS+ VFSSIYNTVKHV
Sbjct: 181 SDLADPHEGGPCNHLYTKSFYEEGIKPKLNDNGIFVTQAGPSGILSYNVFSSIYNTVKHV 240

Query: 241 FRYVIAYTAHVPSYADSCGWVLACDHPLKLDIEELNNRIRERVRGELHYLDGAFIVSSTV 300
           F+YVIAYTAH+PSYADS GWVLA DHP KLD+E+L+ RIRERVRGEL YLDGAFIVSSTV
Sbjct: 241 FKYVIAYTAHIPSYADSSGWVLASDHPFKLDVEDLDKRIRERVRGELRYLDGAFIVSSTV 300

Query: 301 INKTIRTAMMNETHLFTEEDARFAHGQGLVGNA 334
           INKTIRTAMMNETH+FTEEDARF HGQGL  +A
Sbjct: 301 INKTIRTAMMNETHVFTEEDARFTHGQGLASHA 330

[Close]

BLAST of Lcy08g019030 vs. TAIR 10
Match: AT5G19530.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 428.7 bits (1101), Expect = 4.2e-120
Identity = 199/314 (63.38%), Postives = 248/314 (78.98%), Query Frame = 0


Query: 17  STALTLDNVHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQEIVLLDTMRFGKALLLDG 76
           S   TL +  Q   W+EE ++DDLKWSFA+N +LH  TSE+Q+I LLDT RFGK L++DG
Sbjct: 21  SPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDG 80

Query: 77  KLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARETLRHKDIEKVVMCDIDR 136
           K+QSAE+DEFIYHE L+HPALL H NPKTVFIMGGGEG  ARE L+H  IEKVVMCDID+
Sbjct: 81  KMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQ 140

Query: 137 EVVNFCREHLTENRDAFQDEKLHIIFDDAKAGLEGRPEKFDVIIGDLSDPHEGGPCNHLY 196
           EVV+FCR  LT N DAF ++KL ++  DAKA LE R EKFD+I+GDL+DP EGGPC  LY
Sbjct: 141 EVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLY 200

Query: 197 TKSFYEEVIKPKLNDNGIFVTQAGPAGILSHK-VFSSIYNTVKHVFRYVIAYTAHVPSYA 256
           TKSFY+ ++KPKL+ NGIFVTQAGPAGI +HK VF+SIYNT+K VF+YV AYTAHVPS+A
Sbjct: 201 TKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFA 260

Query: 257 DSCGWVLACDHPLKLDIEELNNRIRERVRGELHYLDGAFIVSSTVINKTIRTAMMNETHL 316
           D+ GWV+A DH   ++++E++ RI ERV GEL YL+    VS+  +NKTI  A+  ET +
Sbjct: 261 DTWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALEKETEV 320

Query: 317 FTEEDARFAHGQGL 330
           ++EE+ARF HG G+
Sbjct: 321 YSEENARFIHGHGV 334

[Close]

BLAST of Lcy08g019030 vs. TAIR 10
Match: AT5G19530.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 428.7 bits (1101), Expect = 4.2e-120
Identity = 199/314 (63.38%), Postives = 248/314 (78.98%), Query Frame = 0


Query: 17  STALTLDNVHQVSEWFEEQLEDDLKWSFAVNRILHAATSEFQEIVLLDTMRFGKALLLDG 76
           S   TL +  Q   W+EE ++DDLKWSFA+N +LH  TSE+Q+I LLDT RFGK L++DG
Sbjct: 14  SPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQDIALLDTKRFGKVLVIDG 73

Query: 77  KLQSAEKDEFIYHESLVHPALLLHHNPKTVFIMGGGEGCTARETLRHKDIEKVVMCDIDR 136
           K+QSAE+DEFIYHE L+HPALL H NPKTVFIMGGGEG  ARE L+H  IEKVVMCDID+
Sbjct: 74  KMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAREILKHTTIEKVVMCDIDQ 133

Query: 137 EVVNFCREHLTENRDAFQDEKLHIIFDDAKAGLEGRPEKFDVIIGDLSDPHEGGPCNHLY 196
           EVV+FCR  LT N DAF ++KL ++  DAKA LE R EKFD+I+GDL+DP EGGPC  LY
Sbjct: 134 EVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDIIVGDLADPVEGGPCYQLY 193

Query: 197 TKSFYEEVIKPKLNDNGIFVTQAGPAGILSHK-VFSSIYNTVKHVFRYVIAYTAHVPSYA 256
           TKSFY+ ++KPKL+ NGIFVTQAGPAGI +HK VF+SIYNT+K VF+YV AYTAHVPS+A
Sbjct: 194 TKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTMKQVFKYVKAYTAHVPSFA 253

Query: 257 DSCGWVLACDHPLKLDIEELNNRIRERVRGELHYLDGAFIVSSTVINKTIRTAMMNETHL 316
           D+ GWV+A DH   ++++E++ RI ERV GEL YL+    VS+  +NKTI  A+  ET +
Sbjct: 254 DTWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVSAATLNKTISLALEKETEV 313

Query: 317 FTEEDARFAHGQGL 330
           ++EE+ARF HG G+
Sbjct: 314 YSEENARFIHGHGV 327

[Close]

BLAST of Lcy08g019030 vs. TAIR 10
Match: AT1G23820.2 (spermidine synthase 1 )

HSP 1 Score: 131.0 bits (328), Expect = 1.8e-30
Identity = 71/224 (31.70%), Postives = 119/224 (53.12%), Query Frame = 0


Query: 43  SFAVNRILHAATSEFQEIVLLDTMRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHN 102
           S  V ++L    S++Q++++  +  +GK L+LDG +Q  E+DE  Y E + H  L    N
Sbjct: 105 SLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPN 164

Query: 103 PKTVFIMGGGEGCTARETLRHKDIEKVVMCDIDREVVNFCREHLTENRDAFQDEKLHIIF 162
           PK V ++GGG+G   RE  RH  IE++ MC+ID+ VV+  ++   +    ++D +++++ 
Sbjct: 165 PKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVI 224

Query: 163 DDAKAGLEGRPE-KFDVIIGDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGP 222
            D  A L+   E  +D +I D SDP   GP   L+ K F++ V +  L   G+  TQA  
Sbjct: 225 GDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQSVAR-ALRPGGVVCTQAES 284

Query: 223 AGILSHKVFSSIYNTVKHVFRYVIAYT-AHVPSYADSCG-WVLA 264
              L   +   I +  + +F+  + Y    VP+Y    G W+ A
Sbjct: 285 LW-LHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPRHWGHWIYA 324

[Close]

BLAST of Lcy08g019030 vs. TAIR 10
Match: AT1G23820.1 (spermidine synthase 1 )

HSP 1 Score: 131.0 bits (328), Expect = 1.8e-30
Identity = 74/242 (30.58%), Postives = 125/242 (51.65%), Query Frame = 0


Query: 43  SFAVNRILHAATSEFQEIVLLDTMRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHN 102
           S  V ++L    S++Q++++  +  +GK L+LDG +Q  E+DE  Y E + H  L    N
Sbjct: 105 SLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPN 164

Query: 103 PKTVFIMGGGEGCTARETLRHKDIEKVVMCDIDREVVNFCREHLTENRDAFQDEKLHIIF 162
           PK V ++GGG+G   RE  RH  IE++ MC+ID+ VV+  ++   +    ++D +++++ 
Sbjct: 165 PKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVI 224

Query: 163 DDAKAGLEGRPE-KFDVIIGDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGP 222
            D  A L+   E  +D +I D SDP   GP   L+ K F++ V +  L   G+  TQA  
Sbjct: 225 GDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQSVAR-ALRPGGVVCTQAES 284

Query: 223 AGILSHKVFSSIYNTVKHVFRYVIAYT-AHVPSYADS-CGWVLACDHPLKLDIEELNNRI 282
              L   +   I +  + +F+  + Y    VP+Y     G++L       +D +   N I
Sbjct: 285 LW-LHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPSGVIGFMLCSTEGPDVDFKHPLNPI 342

[Close]

BLAST of Lcy08g019030 vs. TAIR 10
Match: AT1G70310.1 (spermidine synthase 2 )

HSP 1 Score: 130.2 bits (326), Expect = 3.1e-30
Identity = 72/235 (30.64%), Postives = 124/235 (52.77%), Query Frame = 0


Query: 43  SFAVNRILHAATSEFQEIVLLDTMRFGKALLLDGKLQSAEKDEFIYHESLVHPALLLHHN 102
           S  V +IL    S++Q++++  +  +GK L+LDG +Q  E+DE  Y E + H  L    N
Sbjct: 65  SLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSISN 124

Query: 103 PKTVFIMGGGEGCTARETLRHKDIEKVVMCDIDREVVNFCREHLTENRDAFQDEKLHIIF 162
           PK V ++GGG+G   RE  RH  +E++ +C+ID+ VV+  +++       ++D ++++I 
Sbjct: 125 PKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVAKQYFPNVAVGYEDPRVNLII 184

Query: 163 DDAKAGLEGRPE-KFDVIIGDLSDPHEGGPCNHLYTKSFYEEVIKPKLNDNGIFVTQAGP 222
            D  A L+   E  +D +I D SDP   GP   L+ K F+E V +  L   G+  TQA  
Sbjct: 185 GDGVAFLKNAAEGTYDAVIVDSSDPI--GPAKELFEKPFFESVNR-ALRPGGVVCTQAES 244

Query: 223 AGILSHKVFSSIYNTVKHVFRYVIAYT-AHVPSYADS-CGWVLACDHPLKLDIEE 275
              L   +   I +  + +F+  + Y    VP+Y     G++L      ++D ++
Sbjct: 245 LW-LHMDIIEDIVSNCRDIFKGSVNYAWTSVPTYPSGVIGFMLCSSEGPQVDFKK 295

The following BLAST results are available for this feature:

BLAST of Lcy08g019030 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S7X6	6.0e-119	63.38	Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana OX=3702 GN=ACL5 PE=1 SV...	[more]
Q975S5	2.9e-57	41.58	Polyamine aminopropyltransferase OS=Sulfurisphaera tokodaii (strain DSM 16993 / ...	[more]
Q9YE02	1.8e-54	40.80	Polyamine aminopropyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 1...	[more]
Q4JAZ8	1.1e-53	41.58	Polyamine aminopropyltransferase OS=Sulfolobus acidocaldarius (strain ATCC 33909...	[more]
Q72K55	2.6e-53	39.02	Polyamine aminopropyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / ...	[more]

BLAST of Lcy08g019030 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LET5	7.0e-179	90.09	PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G000240 PE=3 S...	[more]
A0A5A7UZV1	2.5e-176	89.49	Thermospermine synthase ACAULIS5-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A1S3CAR3	2.5e-176	89.49	thermospermine synthase ACAULIS5-like OS=Cucumis melo OX=3656 GN=LOC103498720 PE...	[more]
A0A6J1G4J8	1.4e-163	84.98	thermospermine synthase ACAULIS5-like OS=Cucurbita moschata OX=3662 GN=LOC111450...	[more]
A0A6J1KJ05	4.5e-162	84.08	thermospermine synthase ACAULIS5-like OS=Cucurbita maxima OX=3661 GN=LOC11149366...	[more]

BLAST of Lcy08g019030 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038890496.1	2.1e-182	92.49	thermospermine synthase ACAULIS5-like [Benincasa hispida]	[more]
XP_004141174.1	1.4e-178	90.09	thermospermine synthase ACAULIS5 [Cucumis sativus] >KGN60515.1 hypothetical prot...	[more]
XP_008459675.1	5.1e-176	89.49	PREDICTED: thermospermine synthase ACAULIS5-like [Cucumis melo] >KAA0061473.1 th...	[more]
XP_022946737.1	2.9e-163	84.98	thermospermine synthase ACAULIS5-like [Cucurbita moschata]	[more]
KAG7030499.1	5.0e-163	84.38	Thermospermine synthase ACAULIS5 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Lcy08g019030 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19530.1	4.2e-120	63.38	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT5G19530.2	4.2e-120	63.38	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT1G23820.2	1.8e-30	31.70	spermidine synthase 1	[more]
AT1G23820.1	1.8e-30	30.58	spermidine synthase 1	[more]
AT1G70310.1	3.1e-30	30.64	spermidine synthase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF01564	Spermine_synth	coord: 85..257 e-value: 3.5E-37 score: 127.7
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 83..331 e-value: 1.6E-73 score: 249.4
None	No IPR available	PANTHER	PTHR43317	THERMOSPERMINE SYNTHASE ACAULIS5	coord: 3..329
None	No IPR available	PANTHER	PTHR43317:SF6	THERMOSPERMINE SYNTHASE ACAULIS5-LIKE	coord: 3..329
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 110..218 e-value: 3.05722E-7 score: 46.2691
IPR035246	Spermidine synthase, tetramerisation domain	PFAM	PF17284	Spermine_synt_N	coord: 31..82 e-value: 5.7E-12 score: 45.3
IPR037163	Spermidine synthase, tetramerisation domain superfamily	GENE3D	2.30.140.10	Spermidine synthase, tetramerisation domain	coord: 28..82 e-value: 1.6E-15 score: 59.2
IPR030374	Polyamine biosynthesis domain	PROSITE	PS51006	PABS_2	coord: 29..265 score: 88.033401
IPR001045	Spermidine/spermine synthases	HAMAP	MF_00198	Spermidine_synth	coord: 30..306 score: 30.312145
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 29..307

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy08g019030.1	Lcy08g019030.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0006596	polyamine biosynthetic process
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0010487	thermospermine synthase activity
molecular_function	GO:0003824	catalytic activity