Lcy08g016860 (gene) Sponge gourd (P93075) v1

Overview
NameLcy08g016860
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionMethyltransf_11 domain-containing protein
LocationChr08: 47521137 .. 47526681 (-)
RNA-Seq ExpressionLcy08g016860
SyntenyLcy08g016860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTAATCTTGCTGCTGAAGGGGAAGGGGAAGGGGAAGAATAACCAGAGTTCCGAGAAGCTCTAAATCCATTTTCATGGCGGTTGCTACTTTCTTTACATCTCAAACTTCATACAACAATTGCCATTATCTTCCTCGCGATTCCCGCTTTCGGTCTCCTCTTCGATTGTCCACTCATCGTCGTTCTTTCTCCTCTACCGTCCGGGCGAGCTCGACGCTCGTTATTGAGTCGGTATGCTCCACCTGATCTTTCTTGTTTCAATTATCGATTATAATTCCAATTTTTTTATGACATTGTTGCTAAAATGAGTCTCTGTTGTGTGTTGAAGTCGAATTGTATTGTGATTTGGAGTTGAAGATTCGGAACTGTGTGTTTTGCGATTGAATTCTGCAATAATTTAGAGAGAAGTATGTCGAATACCATCCTGTAAGCACGATTATTTTTTGTTGCTATCAGGATTTAGGCATTCAACAGGATCAGAATTCAAAAATTGACGTGTTTTCATGCCCGGTTTGTTATGAGCCCCTGTTGAGAAAAGGCCCGCCAGGTTTTAACTTGTAAGTAGACTTTATGTGATGTCTATCATCTTCCGATAATTATCGTGTTACATTATATGATATTTTGATATGGGTGAAGTGGAAAACTTGCAGGCCAGCTATTTATAGGTCTGGTTTCAAGTGTAGAAGATGCAATAAGTCATACACTAGCAGAAACACCTTTTTGGATCTCACAGTTACTTCTGGAATGAAGGAATATGTTGAAGTCAAACCTGGGGGCACTGAGTTGTTCCGGTATCTGTCTGATATTTACTTTTCATTTTCTCTTCTTAGTATTCCATTCACTTCTAAGTTTTCCTAATTTGCTTCATCAGTTAAGTATTAAGTTGTTGTCAGTTGTCACCCATCACAATTTGTCTAGTAGAAGGAACGGGTACTATATTATAGCCTTTTGTTTCCCTAAGGGAGACAATTCTCAATTGCTTCTGGTTTTTGTGCTTTGTAGGAGTCCGCTCGTTTCATTTCTGTATGAAAGAGGATGGCGTCAAAACTTCAACCGAAGTGGTTTCCCTGGCCCTGATGAAGAAGTATGCTTCCTCTCAATCATAACACTACCATTCAAATATAAGAAGATTTTTGTATGCCATTCAAAAGATAATTCATGGAGGAAAACAAGTCTCATTGTATTGTTACATGAGTTGCCTTGGTATTGGATATATAGCCTCTTCATTTCCTTGGATAATGTGAATATAGGCTGATATATTTTCTGTAACCATATACTATTGCAGTTTAAAATGGCCATGGAGTATTTTAAATCTGTTGAAGGTGGTCTTTTAGTGGATGCAAGCTGTGGTAGTGGTTTGTTTTCCAGAAAATTTGCAAAATCTGGGTCCTTTTCAGGTGTTATAGCACTTGATTTTTCTGAGAGTATGCTCCTCCAATGTTATGATTTCATCAAGAAAGATGCTGCTCTCTTGAACAGGTAATTTCATTTATGACTTCTATTCTCTTGTTTAATTAATTCCTCAGAACTGTAGCTGATATACCCTGTTGGCTTGTGTATTAAGTCCTCTCAACTTGCAAGAAAGTGAAGGCTATCCTAATTCGTTTATCTTCTTTTGGAAAATAATGCAGTAATCTTGCTCTTGTGAGGGCAGATATCTCTAGGCTTCCTTTTTCATCAGGATCAGTGGATGCTGTTCATGCTGGTGCAGCATTGCATTGTTGGCCATCTCCTTCCAATGCTGTAAGCATTTTGGCTTGTAGGATCAAATTCTATCATTTGATTTCCTACCAGTAGTTGTAATCGATGAATTCAACGAGGTTTTAGAACATCACTGTTGATTTCATCCCATTATTTTCGCTGTACAAATTCTCTTCTCAGTTCTCACCCAGCATACGTGATTGAGATCATTTGAAGTTTCTCTCTCCATGCTTGCCACAATTCCTCTTGGTACTTAGTCCGTTAAAAGGAAGTGATCAAGGCTTTCCCTTCTCAATAGTTGCAATCAATCAATTCATATGGAACAAATATGTCGTTGTGTCTGAAACAACGGCAGGTTGTACTGGCCAATTGGTTTTTTGATATATCTTATTGACTAATGCAGTCCTTTTAAGTAGTTTTAGTTGAAATCATTAGGAATTAGCTCCTGGATATTTTTATATATTTTTAGCAACAATGCATCCATTTGTGATGCTTTCAGCTTATTAGGTTGTTGCTGTTCTGATCATCAAAAAGTTAATATTTTTCTGCTTCTTTTAGTAATGGTTTTCTTTAACTCTTCTTATCAGATTGCTGAGATCACTCGAATAATGCGGAGTGGTGGAGTATTTGTGGGAACCACTTTTTTGCGGTATACTTCTTCTACTCCCTGGTACATTAGATTTTCTAGACAGGTATGCTCTTAATCTATTCCCTCTCATCCTTACACAATATCTGATTAGCAGAAAGTTTAGTTTCACTAGTTATCAAGAAGCAGCTATGATAACCATTTGTATTATCTGATTGATATTTCTCGAGTCATGAACTTGGTTATCATGCATTTACATATATTTTTTTGAGAGAAAATTATTTCATTGATAAATGAAATGATCAGAGGGTTAAAACCCCAAAACCATTATGAGAAAACTTACAAAAAGACCTCCAATTGAGAGTAAAAAAATCAAGACTGATCTTTAAAAGAATGTGAACATTTACACCAATAAAGAGCATTAAAAATAGCTAAATCCAGAAAGTCGTCAAAAGATGAATGAGTGTTGCTGAAAATCCTTCCATTTCTTTCACACCATAAAGACCAAAAGAAAGAACGATTAAAGGCTAGCCAAAGAGTTTCCGCATTTCCTTTGAAAGGATGGCCCGAAAAAACCACAGATAGAATAGCTTGCATAGATCCAAGCATAATCATATTCCAGCCAAACCCATTCAGGATAAAGTTCCAATACTTCTGAGCAAAAACACAGGAGCTAAATAAATGACTTTGAGATTCCACATTGGCTTTACACATAATGCACCAAGAAGGAGAGATAACACTATATGGCATTCTTCTTTGGAGACGCTCCGCCGTATTAATACCACCATGACTAAGCTCCCAAAGAAAATTTTTTATTTTTCTAGGATAGAAATCTTTCCATATAGCTGTAAATAAAGGTTGGTTAGCATGGTCTTGTGGCTTCACCAAAAAATGCATCATAGATCGAACCGAGAAGCTTTTAGATGGATCTAAAGACCATTGCCATGAATCATTCTCCCTCTTTAGAGCAAAAATTTGTAATATATGGGCAAGAGATGCCCAATCCGCAATCTCCGAGTCTTTAAGACATCTTCTGAGACCCAAATCCCAAACATTATTTTCCGAATTCCATAAATCAGCCACCGTTGCATGAGCATTATTAGAAAGCCTATAAATCTGGGAAACTCTTGTAGCAAGGTCTATATTAGTATAGCCCAGATATCATGCCAAAAAGAAATATGATTACCATTACCAAGAATTCTTCGAACAGCGGCTTTACCATATTGTTCAACCTTGAGATCTCCCTCCATGGAGCTTTGAAAGACCGAGAGTCAGCTCACATAGGCCAATTTGGATAAGCAGCTAGATGATAATATTTTGCTTTAATAACCTGCTGCCACAGAGAGCCTTCCTCACATAAATATCGCCAAATCCATTTAGATAAAAGCGATTCATTTCTCTGTTTGATATTTCCAATGCCTAAACCTCCAAAAACAATTGGCATTTGAGTTTTGGCCCAACTCACATTGTGTAATCCACCATTACTTTTTGCACCTTCCCAAAGAAAATCCCTCAGGATTTTTTCAAAAGATTTTACAGTTTGAGTTGCCATTCTGAACAAGGATAGAAAATATATAGGCATATTTGAGAGAGTTGACTGTGTAAGAGTATGCCTACCTCCTTTGGAGAGGAAGAAATATTACCAATTATGAAGTTTATGATGAATCTTTTCCACAATTGGTTGCCAAAACAGAGGACTTTTTGGGTTGCCTCATAATGGAAGTCCAAGATAAGTTGATGGACATGTACCCACTCAACATCCAAATTTTCTAGTCAAGTCATTCATCTCTTCTTGAGAAACTTGAATGCCTAGAAGCTCGCTTTTGCTGTAATTAATATTAAGACCTGATGCATTTTCAAATATATGAATGAATTCCGTCGTTGCATATTTTCCAATAACTCTCGCAATTTGTTGATTCTGTTACTTCAAAATGACAAAAAATGTATCTGGACATCCTTTGAGGTGGTATAAACAAGCTAGCTTCCTAAATAGTATATTGAATTTCTTGCTGGCTCATATTTTTCAGTTTCATTGGACTGAGTATCAATATTTATTAGGGTTGATAAATATGTAATCTACTAGATCCATATATAAACTTCAACTAATAAAAGATATCCATTTATCGGTTGGGGCAAGTTTTTCTCCATTTAAAGATCGACATCTGTGAGGAATTTGTTCAAAAATCACGGTTTTGTTTGCTGGAGATTAAATAACTTACAAACTTACAAAAAAAACCCCACCTAGTAAAGATAATTATGAAACAAATTTAAACTAAAAGCATTAAAACGTGTCAAGTCCCAAACACATTATCCCACAACCTACATAATCCAACCATCATTCTCCTATTTCTTTCTAGTCAAAGAAAATACAGTATGGCAATGAAGCTACTTGTCTGAAAACACAACCTTTTTCTTGGAAAGGAAGATCCTACACATTATCCCACAACCTACATAATCCAACCGTCATTCTCCTGTTTCTTTATAGTCAAAGAAAATACAGTATGGCAATGAAGCTACTTGTCTAAAAACACAACCTTTTTCTTGGAAAGGAAGATCCTAGACGACCTTCCCCATCATGTCGTAGATACCGACCTCTAAACTCTAGTTAACCCTGAACTCTGTCCTAATCCATCAATCGTTCAAATACCATTTGATTCCAAAATAAGCATGTAAATTGCTGCTTACGATCTTATGATAAACAACTGTAACGGCGAAAAGTGAGTCAGTGCTAAGTTTCTCTCTATGAACTATCTGCATTAATGTTTTGTATATGGAGATAATACTGATGAGATTCTAGGTTCCTCTAATACTGAAGGATGCAATCATGCAATGATTTTCACATTTTCTCTATTATCAAGATGGACTTCTGAGCGTTATAAGTTTGTGTGCTGCAGCGTGCTTTCCAGAACTTTGGCTACTTAACAGAGGAAGAAATAGAGGATCTGTGCAAATCCTGCGGTCTGATTAACTATTCAAGCAAAGTTCAGCAGTCTTTCATTATGTTCTCTGCTCAGAAGCCATGAAAGTAAGTTCTCAATCTGATCTTCATTGTGATGGCTGATGGATAATGGATATGTTCAGAATCAAACTCTACAGAATTTTATTGATAATTGAATACAATACCATGTAAATTGTTGGAACTGTAAAGGATAATAGAATTATTTGTTAATATACTCGAACAGTTTTAAGACTGAGAAGAGAGGTGTAATATAATAATATGCTCTCTTTGCATGAATTGATTTGTTTCCATTTTTAGGCA

mRNA sequence

CTTAATCTTGCTGCTGAAGGGGAAGGGGAAGGGGAAGAATAACCAGAGTTCCGAGAAGCTCTAAATCCATTTTCATGGCGGTTGCTACTTTCTTTACATCTCAAACTTCATACAACAATTGCCATTATCTTCCTCGCGATTCCCGCTTTCGGTCTCCTCTTCGATTGTCCACTCATCGTCGTTCTTTCTCCTCTACCGTCCGGGCGAGCTCGACGCTCGTTATTGAGTCGGATTTAGGCATTCAACAGGATCAGAATTCAAAAATTGACGTGTTTTCATGCCCGGTTTGTTATGAGCCCCTGTTGAGAAAAGGCCCGCCAGGTTTTAACTTGCCAGCTATTTATAGGTCTGGTTTCAAGTGTAGAAGATGCAATAAGTCATACACTAGCAGAAACACCTTTTTGGATCTCACAGTTACTTCTGGAATGAAGGAATATGTTGAAGTCAAACCTGGGGGCACTGAGTTGTTCCGGAGTCCGCTCGTTTCATTTCTGTATGAAAGAGGATGGCGTCAAAACTTCAACCGAAGTGGTTTCCCTGGCCCTGATGAAGAATTTAAAATGGCCATGGAGTATTTTAAATCTGTTGAAGGTGGTCTTTTAGTGGATGCAAGCTGTGGTAGTGGTTTGTTTTCCAGAAAATTTGCAAAATCTGGGTCCTTTTCAGGTGTTATAGCACTTGATTTTTCTGAGAGTATGCTCCTCCAATGTTATGATTTCATCAAGAAAGATGCTGCTCTCTTGAACAGTAATCTTGCTCTTGTGAGGGCAGATATCTCTAGGCTTCCTTTTTCATCAGGATCAGTGGATGCTGTTCATGCTGGTGCAGCATTGCATTGTTGGCCATCTCCTTCCAATGCTATTGCTGAGATCACTCGAATAATGCGGAGTGGTGGAGTATTTGTGGGAACCACTTTTTTGCGGTATACTTCTTCTACTCCCTGGTACATTAGATTTTCTAGACAGCGTGCTTTCCAGAACTTTGGCTACTTAACAGAGGAAGAAATAGAGGATCTGTGCAAATCCTGCGGTCTGATTAACTATTCAAGCAAAGTTCAGCAGTCTTTCATTATGTTCTCTGCTCAGAAGCCATGAAAGTAAGTTCTCAATCTGATCTTCATTGTGATGGCTGATGGATAATGGATATGTTCAGAATCAAACTCTACAGAATTTTATTGATAATTGAATACAATACCATGTAAATTGTTGGAACTGTAAAGGATAATAGAATTATTTGTTAATATACTCGAACAGTTTTAAGACTGAGAAGAGAGGTGTAATATAATAATATGCTCTCTTTGCATGAATTGATTTGTTTCCATTTTTAGGCA

Coding sequence (CDS)

ATGGCGGTTGCTACTTTCTTTACATCTCAAACTTCATACAACAATTGCCATTATCTTCCTCGCGATTCCCGCTTTCGGTCTCCTCTTCGATTGTCCACTCATCGTCGTTCTTTCTCCTCTACCGTCCGGGCGAGCTCGACGCTCGTTATTGAGTCGGATTTAGGCATTCAACAGGATCAGAATTCAAAAATTGACGTGTTTTCATGCCCGGTTTGTTATGAGCCCCTGTTGAGAAAAGGCCCGCCAGGTTTTAACTTGCCAGCTATTTATAGGTCTGGTTTCAAGTGTAGAAGATGCAATAAGTCATACACTAGCAGAAACACCTTTTTGGATCTCACAGTTACTTCTGGAATGAAGGAATATGTTGAAGTCAAACCTGGGGGCACTGAGTTGTTCCGGAGTCCGCTCGTTTCATTTCTGTATGAAAGAGGATGGCGTCAAAACTTCAACCGAAGTGGTTTCCCTGGCCCTGATGAAGAATTTAAAATGGCCATGGAGTATTTTAAATCTGTTGAAGGTGGTCTTTTAGTGGATGCAAGCTGTGGTAGTGGTTTGTTTTCCAGAAAATTTGCAAAATCTGGGTCCTTTTCAGGTGTTATAGCACTTGATTTTTCTGAGAGTATGCTCCTCCAATGTTATGATTTCATCAAGAAAGATGCTGCTCTCTTGAACAGTAATCTTGCTCTTGTGAGGGCAGATATCTCTAGGCTTCCTTTTTCATCAGGATCAGTGGATGCTGTTCATGCTGGTGCAGCATTGCATTGTTGGCCATCTCCTTCCAATGCTATTGCTGAGATCACTCGAATAATGCGGAGTGGTGGAGTATTTGTGGGAACCACTTTTTTGCGGTATACTTCTTCTACTCCCTGGTACATTAGATTTTCTAGACAGCGTGCTTTCCAGAACTTTGGCTACTTAACAGAGGAAGAAATAGAGGATCTGTGCAAATCCTGCGGTCTGATTAACTATTCAAGCAAAGTTCAGCAGTCTTTCATTATGTTCTCTGCTCAGAAGCCATGA

Protein sequence

MAVATFFTSQTSYNNCHYLPRDSRFRSPLRLSTHRRSFSSTVRASSTLVIESDLGIQQDQNSKIDVFSCPVCYEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSRNTFLDLTVTSGMKEYVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSVEGGLLVDASCGSGLFSRKFAKSGSFSGVIALDFSESMLLQCYDFIKKDAALLNSNLALVRADISRLPFSSGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRQRAFQNFGYLTEEEIEDLCKSCGLINYSSKVQQSFIMFSAQKP
Homology
BLAST of Lcy08g016860 vs. ExPASy Swiss-Prot
Match: Q0WPT7 (Uncharacterized methyltransferase At2g41040, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g41040 PE=1 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 2.3e-118
Identity = 219/350 (62.57%), Postives = 266/350 (76.00%), Query Frame = 0

Query: 1   MAVATFFTSQTSYNNCHYLP-RDSRFRSPLRLSTHRRSFSSTVR------ASSTLVIESD 60
           MA  T  +S  +  N  +LP R S F S  +    R S S++VR      A S +  +SD
Sbjct: 3   MAALTSSSSAITLLNKPFLPNRSSFFSSDSQSPLLRFSASTSVRSRFPSAAISAVAPKSD 62

Query: 61  LGIQQDQNSKID---VFSCPVCYEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSRNTFL 120
           +   +    +I+   VF+CPVCYEPL+RKGP G NL AIYRSGFKC +CNK+Y+S++ +L
Sbjct: 63  INKNETPKIEIEETQVFACPVCYEPLMRKGPSGINLQAIYRSGFKCGQCNKTYSSKDEYL 122

Query: 121 DLTVTSGMKEYVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKS 180
           DLTVT+ + +Y EVKP  TELFRSPLVSFLYERGWRQ F RSGFPGPDEEF+MA EYFK 
Sbjct: 123 DLTVTADLDDYNEVKPITTELFRSPLVSFLYERGWRQAFKRSGFPGPDEEFRMAEEYFKE 182

Query: 181 VEGGLLVDASCGSGLFSRKFAKSGSFSGVIALDFSESMLLQCYDFIKKDAALLNS-NLAL 240
            EGGLLVD SCGSGLFSRKFA+SG +SGVIALD+SE+ML QC +FIK D    NS N+A+
Sbjct: 183 AEGGLLVDVSCGSGLFSRKFAQSGKYSGVIALDYSENMLRQCKEFIKNDNTFDNSTNIAV 242

Query: 241 VRADISRLPFSSGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTP 300
           VRAD+SRLPF SGSVDAVHAGAALHCWPSP+NAIAEI R++RSGGVFVGTTFLRY+ STP
Sbjct: 243 VRADVSRLPFPSGSVDAVHAGAALHCWPSPTNAIAEICRVLRSGGVFVGTTFLRYSPSTP 302

Query: 301 WYIRFSRQRAFQNFGYLTEEEIEDLCKSCGLINYSSKVQQSFIMFSAQKP 340
           W IR  + R  Q++ YL ++EI+D+C SCGL +Y   +Q SFIMF+A+KP
Sbjct: 303 WIIRPFQSRILQSYNYLMQDEIKDVCTSCGLTDYEDYIQDSFIMFTARKP 352

BLAST of Lcy08g016860 vs. ExPASy Swiss-Prot
Match: Q8LBV4 (Uncharacterized methyltransferase At1g78140, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g78140 PE=1 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 2.8e-79
Identity = 158/315 (50.16%), Postives = 203/315 (64.44%), Query Frame = 0

Query: 32  STHRRSFSSTVRASSTLVIESDLGIQQDQNSKIDVFSCPVCYEPLL-RKGPPGFNLPAIY 91
           S H  S S +V  +S   ++  +  +     +  + +CP+CY  L     P G    A  
Sbjct: 41  SAHASSASVSVETNSNSNVDFVIEKKDKNRGEKKILACPICYNSLAWISQPNGLIESAAS 100

Query: 92  RSGFKCRRCNKSYTSRNTFLDLTVTSGMKEYVEVKPGGTELFRSPLVSFLYERGWRQNFN 151
               +C  C +SY+   T LDL V SG K Y E  P  TELFR+PLVSFLYERGWRQNF 
Sbjct: 101 GIQVQCNTCKRSYSGNETHLDLAVASGSKRYSEPMPLSTELFRTPLVSFLYERGWRQNFI 160

Query: 152 RSGFPGPDEEFKMAMEYFKSVEGGLLVDASCGSGLFSRKFAKSGSFSGVIALDFSESMLL 211
             GFPGP++EF+MA  Y K V GG ++DASCGSG+FSR F +S  FS VIALD+SE+ML 
Sbjct: 161 WGGFPGPEKEFEMAKAYLKPVLGGNIIDASCGSGMFSRLFTRSDLFSLVIALDYSENMLR 220

Query: 212 QCYDFIKKDAALLN-SNLALVRADISRLPFSSGSVDAVHAGAALHCWPSPSNAIAEITRI 271
           QCY+ + K+    N   L LVRADI+RLPF SGSVDAVHAGAALHCWPSPS+A+AEI+R+
Sbjct: 221 QCYELLNKEENFPNKEKLVLVRADIARLPFLSGSVDAVHAGAALHCWPSPSSAVAEISRV 280

Query: 272 MRSGGVFVGTTFLRYTSSTPW--YIRFSRQRAFQNFG---YLTEEEIEDLCKSCGLINYS 331
           +R GGVFV TTF+ Y     +  +++  RQ   +  G   +L E E+ED+CK+CGL+N++
Sbjct: 281 LRPGGVFVATTFI-YDGPFSFIPFLKNLRQEIMRYSGSHIFLNERELEDICKACGLVNFT 340

Query: 332 SKVQQSFIMFSAQKP 340
                 FIM SA KP
Sbjct: 341 RVRNGPFIMLSATKP 354

BLAST of Lcy08g016860 vs. ExPASy Swiss-Prot
Match: O14321 (Sterol 24-C-methyltransferase erg6 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=erg6 PE=2 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.5e-05
Identity = 42/151 (27.81%), Postives = 74/151 (49.01%), Query Frame = 0

Query: 136 LVSFLYERGWRQNFNRSGFPGPDEEFKMAM---EYFKSVEGGL-----LVDASCGSGLFS 195
           L + LYE GW Q+F+ S F    E F  ++   E++ +   G+     ++D  CG G  +
Sbjct: 81  LATDLYEYGWSQSFHFSRF-YKGEAFAQSIARHEHYLAYRMGIKPGSRVLDVGCGVGGPA 140

Query: 196 RKFAKSGSFSG--VIALDFSESMLLQCYDFIKKDAALLNSNLALVRADISRLPFSSGSVD 255
           R+  +   F+G  ++ L+ ++  + +C ++  K    L+     V+ D   +PF   + D
Sbjct: 141 REITE---FTGCNLVGLNNNDYQISRCNNYAVK--RNLDKKQVFVKGDFMHMPFEDNTFD 200

Query: 256 AVHAGAALHCWPSPSNAIAEITRIMRSGGVF 277
            V+A  A    PS      EI R+++ GGVF
Sbjct: 201 YVYAIEATVHAPSLEGVYGEIFRVLKPGGVF 225

BLAST of Lcy08g016860 vs. ExPASy Swiss-Prot
Match: Q5ZT34 (Malonyl-[acyl-carrier protein] O-methyltransferase OS=Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) OX=272624 GN=bioC PE=3 SV=2)

HSP 1 Score: 50.8 bits (120), Expect = 3.4e-05
Identity = 32/105 (30.48%), Postives = 51/105 (48.57%), Query Frame = 0

Query: 176 LVDASCGSGLFSRKFAKSGSFSGVIALDFSESMLLQCYDFIKKDAALLNSNLALVRADIS 235
           ++D  CG G FS++ A     + ++ +D S +ML Q     KK          LV AD+ 
Sbjct: 46  ILDLGCGPGFFSKELALLYPKAQIVGMDLSFAMLEQAR---KKQG--WRRKWPLVSADMQ 105

Query: 236 RLPFSSGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTT 281
           ++PF++G+ D V A   +H   S      E+ R+M   G  + TT
Sbjct: 106 KMPFATGAFDLVFANQVIHWSSSLGMVFRELNRVMNVNGCLMFTT 145

BLAST of Lcy08g016860 vs. ExPASy Swiss-Prot
Match: Q5HP74 (Demethylmenaquinone methyltransferase OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) OX=176279 GN=menG PE=3 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 3.4e-05
Identity = 38/117 (32.48%), Postives = 57/117 (48.72%), Query Frame = 0

Query: 162 KMAMEYFKSVEGGLLVDASCGSGLFSRKFAKS-GSFSGVIALDFSESMLLQCYDFIKKDA 221
           K  M      +G   +D  CG+  ++   +++ GS   V  LDFSE+ML    +  K+  
Sbjct: 39  KHVMSTMNVQKGSKALDVCCGTADWTIALSEAVGSKGQVTGLDFSENML----EVGKQKT 98

Query: 222 ALLNSNLALVRADISRLPFSSGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFV 278
           A L  N+ LV  D   LPF   S D V  G  L   P   +A+ E+ R+++ GG+ V
Sbjct: 99  ASL-ENIQLVHGDAMNLPFDDNSFDYVTIGFGLRNVPDYLSALKEMHRVLKPGGMVV 150

BLAST of Lcy08g016860 vs. ExPASy TrEMBL
Match: A0A6J1CMF3 (uncharacterized methyltransferase At2g41040, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111012967 PE=4 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 1.2e-178
Identity = 316/339 (93.22%), Postives = 326/339 (96.17%), Query Frame = 0

Query: 1   MAVATFFTSQTSYNNCHYLPRDSRFRSPLRLSTHRRSFSSTVRASSTLVIESDLGIQQDQ 60
           MAVAT F SQTSYNN  YL RDSRFRSPLRLST RRSF S +RASSTLV+ESDLGIQQDQ
Sbjct: 1   MAVATLFPSQTSYNNRRYLSRDSRFRSPLRLST-RRSF-SFIRASSTLVLESDLGIQQDQ 60

Query: 61  NSKIDVFSCPVCYEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSRNTFLDLTVTSGMKE 120
           NSK+D+FSCPVCYEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTS+N FLDLTVT+GM++
Sbjct: 61  NSKVDLFSCPVCYEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTAGMRD 120

Query: 121 YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSVEGGLLVDAS 180
           YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKS EGGLLVDAS
Sbjct: 121 YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSAEGGLLVDAS 180

Query: 181 CGSGLFSRKFAKSGSFSGVIALDFSESMLLQCYDFIKKDAALLNSNLALVRADISRLPFS 240
           CGSGLFSRKFAKSGSFSGV+ALDFSE+MLLQCYDFIKKDAALLNSNLALVRADISRLPFS
Sbjct: 181 CGSGLFSRKFAKSGSFSGVVALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFS 240

Query: 241 SGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRQRAF 300
           SGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRQR  
Sbjct: 241 SGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRQRFS 300

Query: 301 QNFGYLTEEEIEDLCKSCGLINYSSKVQQSFIMFSAQKP 340
           Q FGYLTEEEIEDLCKSCGLINYSSKVQQSFIMFSAQKP
Sbjct: 301 QEFGYLTEEEIEDLCKSCGLINYSSKVQQSFIMFSAQKP 337

BLAST of Lcy08g016860 vs. ExPASy TrEMBL
Match: A0A6J1HLD0 (uncharacterized methyltransferase At2g41040, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111465594 PE=4 SV=1)

HSP 1 Score: 630.9 bits (1626), Expect = 3.0e-177
Identity = 310/339 (91.45%), Postives = 324/339 (95.58%), Query Frame = 0

Query: 1   MAVATFFTSQTSYNNCHYLPRDSRFRSPLRLSTHRRSFSSTVRASSTLVIESDLGIQQDQ 60
           MA ATFF  QTS+NN HYL RD+RFRSPLR+ST RRSFS TVRASSTLV+ESDL IQQD+
Sbjct: 1   MAAATFFPYQTSFNNRHYLSRDTRFRSPLRVST-RRSFSFTVRASSTLVLESDLSIQQDR 60

Query: 61  NSKIDVFSCPVCYEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSRNTFLDLTVTSGMKE 120
           NSKIDVFSCPVC+EPLLR GPPGFNLPAIYRSGFKC RCNKSYTS+N FLDLTVTSGMKE
Sbjct: 61  NSKIDVFSCPVCFEPLLRTGPPGFNLPAIYRSGFKCGRCNKSYTSKNIFLDLTVTSGMKE 120

Query: 121 YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSVEGGLLVDAS 180
           YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKS EGGLLVDAS
Sbjct: 121 YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSAEGGLLVDAS 180

Query: 181 CGSGLFSRKFAKSGSFSGVIALDFSESMLLQCYDFIKKDAALLNSNLALVRADISRLPFS 240
           CGSGLFSRKFAKSG+FSGVIALDFSE+MLLQCYDFIKKDA LLNSNLALVRADISRLPFS
Sbjct: 181 CGSGLFSRKFAKSGAFSGVIALDFSENMLLQCYDFIKKDATLLNSNLALVRADISRLPFS 240

Query: 241 SGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRQRAF 300
           SGSVDAVHAGAALHCWPSPSNAIAEITR MRSGGVFVGTTFLRYTSSTPW++R SR+RAF
Sbjct: 241 SGSVDAVHAGAALHCWPSPSNAIAEITRAMRSGGVFVGTTFLRYTSSTPWFVRISRERAF 300

Query: 301 QNFGYLTEEEIEDLCKSCGLINYSSKVQQSFIMFSAQKP 340
           QNFGYLTE+EIEDLCKSCGLINYSSK+QQSFIMFSAQKP
Sbjct: 301 QNFGYLTEKEIEDLCKSCGLINYSSKIQQSFIMFSAQKP 338

BLAST of Lcy08g016860 vs. ExPASy TrEMBL
Match: A0A1S3C4D9 (uncharacterized methyltransferase At2g41040, chloroplastic isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496525 PE=4 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 6.2e-175
Identity = 308/339 (90.86%), Postives = 322/339 (94.99%), Query Frame = 0

Query: 1   MAVATFFTSQTSYNNCHYLPRDSRFRSPLRLSTHRRSFSSTVRASSTLVIESDLGIQQDQ 60
           MAVATFF SQTSYNN HYL R+ +FR PLRLST  RSF ST+RASS LV+ESD G QQDQ
Sbjct: 1   MAVATFFPSQTSYNNSHYLSRNIQFRYPLRLST-PRSFLSTIRASSALVLESDSGTQQDQ 60

Query: 61  NSKIDVFSCPVCYEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSRNTFLDLTVTSGMKE 120
           NSKIDVFSCPVC+EPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTS+N FLDLTVTSGMKE
Sbjct: 61  NSKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKE 120

Query: 121 YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSVEGGLLVDAS 180
           YVEVKPGGTELFRSPLVS+LYERGWRQNFN+SGFPG DEEFKMAMEYFKSVEGGLLVDAS
Sbjct: 121 YVEVKPGGTELFRSPLVSYLYERGWRQNFNQSGFPGLDEEFKMAMEYFKSVEGGLLVDAS 180

Query: 181 CGSGLFSRKFAKSGSFSGVIALDFSESMLLQCYDFIKKDAALLNSNLALVRADISRLPFS 240
           CGSGLFSRKFAKSGSFSGVIALDFSE+MLLQCYDFIKKDA LLNSNLALVRADISRLPFS
Sbjct: 181 CGSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDATLLNSNLALVRADISRLPFS 240

Query: 241 SGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRQRAF 300
           SGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWY+RF R+R F
Sbjct: 241 SGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYLRFLRERGF 300

Query: 301 QNFGYLTEEEIEDLCKSCGLINYSSKVQQSFIMFSAQKP 340
           Q +GYLTEEEIE+LCKSCGLINYSSKVQ+SFIMFSAQKP
Sbjct: 301 QPYGYLTEEEIEELCKSCGLINYSSKVQRSFIMFSAQKP 338

BLAST of Lcy08g016860 vs. ExPASy TrEMBL
Match: A0A6J1FCL5 (uncharacterized methyltransferase At2g41040, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111444178 PE=4 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 1.1e-174
Identity = 306/339 (90.27%), Postives = 323/339 (95.28%), Query Frame = 0

Query: 1   MAVATFFTSQTSYNNCHYLPRDSRFRSPLRLSTHRRSFSSTVRASSTLVIESDLGIQQDQ 60
           MAVATFF  QT+ NN H+L  D+RFRSPLR+S+ RRSFS TVRASSTLV+ESDL IQQD+
Sbjct: 1   MAVATFFPCQTAINNRHFLSLDTRFRSPLRVSS-RRSFSFTVRASSTLVLESDLSIQQDR 60

Query: 61  NSKIDVFSCPVCYEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSRNTFLDLTVTSGMKE 120
           NSKIDVFSCPVC+EPLLRKGPPGFNLPAIYRSGFKC RCNKSYTS+N FLDLTVTSGMKE
Sbjct: 61  NSKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCGRCNKSYTSKNIFLDLTVTSGMKE 120

Query: 121 YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSVEGGLLVDAS 180
           YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKS E GLLVDAS
Sbjct: 121 YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSAEDGLLVDAS 180

Query: 181 CGSGLFSRKFAKSGSFSGVIALDFSESMLLQCYDFIKKDAALLNSNLALVRADISRLPFS 240
           CGSGLFSRKFAKS +FSGVIALDFSE+MLLQCYDFIKKDA LLNSNLALVRADISRLPFS
Sbjct: 181 CGSGLFSRKFAKSEAFSGVIALDFSENMLLQCYDFIKKDATLLNSNLALVRADISRLPFS 240

Query: 241 SGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRQRAF 300
           SGSVDAVHAGAALHCWPSPSNAIAEITR+MRSGGVFVGTTFLRYTSSTPW++R SR+RAF
Sbjct: 241 SGSVDAVHAGAALHCWPSPSNAIAEITRVMRSGGVFVGTTFLRYTSSTPWFVRISRERAF 300

Query: 301 QNFGYLTEEEIEDLCKSCGLINYSSKVQQSFIMFSAQKP 340
           QNFGYLTE+EIEDLCKSCGLINYSSK+QQSFIMFSAQKP
Sbjct: 301 QNFGYLTEKEIEDLCKSCGLINYSSKIQQSFIMFSAQKP 338

BLAST of Lcy08g016860 vs. ExPASy TrEMBL
Match: A0A5A7VAW1 (Putative methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G00970 PE=4 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 6.9e-174
Identity = 309/340 (90.88%), Postives = 322/340 (94.71%), Query Frame = 0

Query: 1   MAVATFFTSQTSYNNCHYLPRDSRFRSPLRLSTHRRSFSSTVRASSTLVIESDLGIQQDQ 60
           MAVATFF SQTSYNN HYL R+ +FR PLRLST  RSF ST+RASS LV+ESD G QQDQ
Sbjct: 1   MAVATFFPSQTSYNNSHYLSRNIQFRYPLRLST-PRSFLSTIRASSALVLESDSGTQQDQ 60

Query: 61  NSKIDVFSCPVCYEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSRNTFLDLTVTSGMKE 120
           NSKIDVFSCPVC+EPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTS+N FLDLTVTSGMKE
Sbjct: 61  NSKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKE 120

Query: 121 YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSVEGGLLVDAS 180
           YVEVKPGGTELFRSPLVS+LYERGWRQNFN+SGFPG DEEFKMAMEYFKSVEGGLLVDAS
Sbjct: 121 YVEVKPGGTELFRSPLVSYLYERGWRQNFNQSGFPGLDEEFKMAMEYFKSVEGGLLVDAS 180

Query: 181 CGSGLFSRKFAKSGSFSGVIALDFSESMLLQCYDFIKKDAALLNSNLALVRADISRLPFS 240
           CGSGLFSRKFAKSGSFSGVIALDFSE+MLLQCYDFIKKDA LLNSNLALVRADISRLPFS
Sbjct: 181 CGSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDATLLNSNLALVRADISRLPFS 240

Query: 241 SGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSR-QRA 300
           SGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWY+RF R QR 
Sbjct: 241 SGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYLRFLREQRG 300

Query: 301 FQNFGYLTEEEIEDLCKSCGLINYSSKVQQSFIMFSAQKP 340
           FQ +GYLTEEEIE+LCKSCGLINYSSKVQ+SFIMFSAQKP
Sbjct: 301 FQPYGYLTEEEIEELCKSCGLINYSSKVQRSFIMFSAQKP 339

BLAST of Lcy08g016860 vs. NCBI nr
Match: XP_038889838.1 (uncharacterized methyltransferase At2g41040, chloroplastic [Benincasa hispida])

HSP 1 Score: 642.1 bits (1655), Expect = 2.7e-180
Identity = 319/339 (94.10%), Postives = 327/339 (96.46%), Query Frame = 0

Query: 1   MAVATFFTSQTSYNNCHYLPRDSRFRSPLRLSTHRRSFSSTVRASSTLVIESDLGIQQDQ 60
           MAVATFF SQTSYNN H+L RD RFRSPLR ST RRSF STVRASS LV+ESDLGIQQDQ
Sbjct: 1   MAVATFFPSQTSYNNRHFLSRDIRFRSPLRFST-RRSFFSTVRASSGLVLESDLGIQQDQ 60

Query: 61  NSKIDVFSCPVCYEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSRNTFLDLTVTSGMKE 120
           NSKIDVFSCPVCYEPLLRKGPPG NLPAIYRSGFKCRRCNK+YTS+N FLDLTVTSGMKE
Sbjct: 61  NSKIDVFSCPVCYEPLLRKGPPGLNLPAIYRSGFKCRRCNKTYTSKNIFLDLTVTSGMKE 120

Query: 121 YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSVEGGLLVDAS 180
           YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPG DEEFKMAMEYFKSVEGGLLVDAS
Sbjct: 121 YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGLDEEFKMAMEYFKSVEGGLLVDAS 180

Query: 181 CGSGLFSRKFAKSGSFSGVIALDFSESMLLQCYDFIKKDAALLNSNLALVRADISRLPFS 240
           CGSGLFSRKFAKSGSFSGVIALDFSE+MLLQCYDFIKKD+ LLNSNLALVRADISRLPFS
Sbjct: 181 CGSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDSTLLNSNLALVRADISRLPFS 240

Query: 241 SGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRQRAF 300
           SGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSR+RAF
Sbjct: 241 SGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRERAF 300

Query: 301 QNFGYLTEEEIEDLCKSCGLINYSSKVQQSFIMFSAQKP 340
           QNFGYLTEEEIEDLCKSCGLINYSSK+QQSFIMFSAQKP
Sbjct: 301 QNFGYLTEEEIEDLCKSCGLINYSSKMQQSFIMFSAQKP 338

BLAST of Lcy08g016860 vs. NCBI nr
Match: XP_022142970.1 (uncharacterized methyltransferase At2g41040, chloroplastic [Momordica charantia])

HSP 1 Score: 635.6 bits (1638), Expect = 2.5e-178
Identity = 316/339 (93.22%), Postives = 326/339 (96.17%), Query Frame = 0

Query: 1   MAVATFFTSQTSYNNCHYLPRDSRFRSPLRLSTHRRSFSSTVRASSTLVIESDLGIQQDQ 60
           MAVAT F SQTSYNN  YL RDSRFRSPLRLST RRSF S +RASSTLV+ESDLGIQQDQ
Sbjct: 1   MAVATLFPSQTSYNNRRYLSRDSRFRSPLRLST-RRSF-SFIRASSTLVLESDLGIQQDQ 60

Query: 61  NSKIDVFSCPVCYEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSRNTFLDLTVTSGMKE 120
           NSK+D+FSCPVCYEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTS+N FLDLTVT+GM++
Sbjct: 61  NSKVDLFSCPVCYEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTAGMRD 120

Query: 121 YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSVEGGLLVDAS 180
           YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKS EGGLLVDAS
Sbjct: 121 YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSAEGGLLVDAS 180

Query: 181 CGSGLFSRKFAKSGSFSGVIALDFSESMLLQCYDFIKKDAALLNSNLALVRADISRLPFS 240
           CGSGLFSRKFAKSGSFSGV+ALDFSE+MLLQCYDFIKKDAALLNSNLALVRADISRLPFS
Sbjct: 181 CGSGLFSRKFAKSGSFSGVVALDFSENMLLQCYDFIKKDAALLNSNLALVRADISRLPFS 240

Query: 241 SGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRQRAF 300
           SGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRQR  
Sbjct: 241 SGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRQRFS 300

Query: 301 QNFGYLTEEEIEDLCKSCGLINYSSKVQQSFIMFSAQKP 340
           Q FGYLTEEEIEDLCKSCGLINYSSKVQQSFIMFSAQKP
Sbjct: 301 QEFGYLTEEEIEDLCKSCGLINYSSKVQQSFIMFSAQKP 337

BLAST of Lcy08g016860 vs. NCBI nr
Match: XP_023536953.1 (uncharacterized methyltransferase At2g41040, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 632.1 bits (1629), Expect = 2.8e-177
Identity = 310/339 (91.45%), Postives = 326/339 (96.17%), Query Frame = 0

Query: 1   MAVATFFTSQTSYNNCHYLPRDSRFRSPLRLSTHRRSFSSTVRASSTLVIESDLGIQQDQ 60
           MAVATFF  QTS NN H+L RD+RFRSPLR+ST RRSFS TVRASSTLV+ESDL IQQD+
Sbjct: 1   MAVATFFPYQTSINNRHFLSRDTRFRSPLRVST-RRSFSFTVRASSTLVLESDLSIQQDR 60

Query: 61  NSKIDVFSCPVCYEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSRNTFLDLTVTSGMKE 120
           N+KIDVFSCPVC+EPLLRKGPPGFNLPAIYRSGFKC RCNKSYTS+N FLDLTVTSGMKE
Sbjct: 61  NAKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCGRCNKSYTSKNIFLDLTVTSGMKE 120

Query: 121 YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSVEGGLLVDAS 180
           YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKS EGGLLVDAS
Sbjct: 121 YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSAEGGLLVDAS 180

Query: 181 CGSGLFSRKFAKSGSFSGVIALDFSESMLLQCYDFIKKDAALLNSNLALVRADISRLPFS 240
           CGSGLFSRKFAKSG+FSGVIALDFSE+MLLQCYDFIKKDA LLNSNLALVRADISRLPFS
Sbjct: 181 CGSGLFSRKFAKSGAFSGVIALDFSENMLLQCYDFIKKDATLLNSNLALVRADISRLPFS 240

Query: 241 SGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRQRAF 300
           SGSVDAVHAGAALHCWPSPSNAIAEITR+MRSGGVFVGTTFLRYTSSTPW++R SR+RAF
Sbjct: 241 SGSVDAVHAGAALHCWPSPSNAIAEITRVMRSGGVFVGTTFLRYTSSTPWFVRISRERAF 300

Query: 301 QNFGYLTEEEIEDLCKSCGLINYSSKVQQSFIMFSAQKP 340
           QNFGYLTE+EIEDLCKSCGLINYSSK+QQSFIMFSAQKP
Sbjct: 301 QNFGYLTEKEIEDLCKSCGLINYSSKIQQSFIMFSAQKP 338

BLAST of Lcy08g016860 vs. NCBI nr
Match: XP_022965827.1 (uncharacterized methyltransferase At2g41040, chloroplastic [Cucurbita maxima])

HSP 1 Score: 630.9 bits (1626), Expect = 6.2e-177
Identity = 310/339 (91.45%), Postives = 324/339 (95.58%), Query Frame = 0

Query: 1   MAVATFFTSQTSYNNCHYLPRDSRFRSPLRLSTHRRSFSSTVRASSTLVIESDLGIQQDQ 60
           MA ATFF  QTS+NN HYL RD+RFRSPLR+ST RRSFS TVRASSTLV+ESDL IQQD+
Sbjct: 1   MAAATFFPYQTSFNNRHYLSRDTRFRSPLRVST-RRSFSFTVRASSTLVLESDLSIQQDR 60

Query: 61  NSKIDVFSCPVCYEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSRNTFLDLTVTSGMKE 120
           NSKIDVFSCPVC+EPLLR GPPGFNLPAIYRSGFKC RCNKSYTS+N FLDLTVTSGMKE
Sbjct: 61  NSKIDVFSCPVCFEPLLRTGPPGFNLPAIYRSGFKCGRCNKSYTSKNIFLDLTVTSGMKE 120

Query: 121 YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSVEGGLLVDAS 180
           YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKS EGGLLVDAS
Sbjct: 121 YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSAEGGLLVDAS 180

Query: 181 CGSGLFSRKFAKSGSFSGVIALDFSESMLLQCYDFIKKDAALLNSNLALVRADISRLPFS 240
           CGSGLFSRKFAKSG+FSGVIALDFSE+MLLQCYDFIKKDA LLNSNLALVRADISRLPFS
Sbjct: 181 CGSGLFSRKFAKSGAFSGVIALDFSENMLLQCYDFIKKDATLLNSNLALVRADISRLPFS 240

Query: 241 SGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRQRAF 300
           SGSVDAVHAGAALHCWPSPSNAIAEITR MRSGGVFVGTTFLRYTSSTPW++R SR+RAF
Sbjct: 241 SGSVDAVHAGAALHCWPSPSNAIAEITRAMRSGGVFVGTTFLRYTSSTPWFVRISRERAF 300

Query: 301 QNFGYLTEEEIEDLCKSCGLINYSSKVQQSFIMFSAQKP 340
           QNFGYLTE+EIEDLCKSCGLINYSSK+QQSFIMFSAQKP
Sbjct: 301 QNFGYLTEKEIEDLCKSCGLINYSSKIQQSFIMFSAQKP 338

BLAST of Lcy08g016860 vs. NCBI nr
Match: XP_008456635.1 (PREDICTED: uncharacterized methyltransferase At2g41040, chloroplastic isoform X2 [Cucumis melo])

HSP 1 Score: 623.2 bits (1606), Expect = 1.3e-174
Identity = 308/339 (90.86%), Postives = 322/339 (94.99%), Query Frame = 0

Query: 1   MAVATFFTSQTSYNNCHYLPRDSRFRSPLRLSTHRRSFSSTVRASSTLVIESDLGIQQDQ 60
           MAVATFF SQTSYNN HYL R+ +FR PLRLST  RSF ST+RASS LV+ESD G QQDQ
Sbjct: 1   MAVATFFPSQTSYNNSHYLSRNIQFRYPLRLST-PRSFLSTIRASSALVLESDSGTQQDQ 60

Query: 61  NSKIDVFSCPVCYEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSRNTFLDLTVTSGMKE 120
           NSKIDVFSCPVC+EPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTS+N FLDLTVTSGMKE
Sbjct: 61  NSKIDVFSCPVCFEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSKNIFLDLTVTSGMKE 120

Query: 121 YVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKSVEGGLLVDAS 180
           YVEVKPGGTELFRSPLVS+LYERGWRQNFN+SGFPG DEEFKMAMEYFKSVEGGLLVDAS
Sbjct: 121 YVEVKPGGTELFRSPLVSYLYERGWRQNFNQSGFPGLDEEFKMAMEYFKSVEGGLLVDAS 180

Query: 181 CGSGLFSRKFAKSGSFSGVIALDFSESMLLQCYDFIKKDAALLNSNLALVRADISRLPFS 240
           CGSGLFSRKFAKSGSFSGVIALDFSE+MLLQCYDFIKKDA LLNSNLALVRADISRLPFS
Sbjct: 181 CGSGLFSRKFAKSGSFSGVIALDFSENMLLQCYDFIKKDATLLNSNLALVRADISRLPFS 240

Query: 241 SGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYIRFSRQRAF 300
           SGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWY+RF R+R F
Sbjct: 241 SGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTPWYLRFLRERGF 300

Query: 301 QNFGYLTEEEIEDLCKSCGLINYSSKVQQSFIMFSAQKP 340
           Q +GYLTEEEIE+LCKSCGLINYSSKVQ+SFIMFSAQKP
Sbjct: 301 QPYGYLTEEEIEELCKSCGLINYSSKVQRSFIMFSAQKP 338

BLAST of Lcy08g016860 vs. TAIR 10
Match: AT2G41040.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 426.8 bits (1096), Expect = 1.6e-119
Identity = 219/350 (62.57%), Postives = 266/350 (76.00%), Query Frame = 0

Query: 1   MAVATFFTSQTSYNNCHYLP-RDSRFRSPLRLSTHRRSFSSTVR------ASSTLVIESD 60
           MA  T  +S  +  N  +LP R S F S  +    R S S++VR      A S +  +SD
Sbjct: 3   MAALTSSSSAITLLNKPFLPNRSSFFSSDSQSPLLRFSASTSVRSRFPSAAISAVAPKSD 62

Query: 61  LGIQQDQNSKID---VFSCPVCYEPLLRKGPPGFNLPAIYRSGFKCRRCNKSYTSRNTFL 120
           +   +    +I+   VF+CPVCYEPL+RKGP G NL AIYRSGFKC +CNK+Y+S++ +L
Sbjct: 63  INKNETPKIEIEETQVFACPVCYEPLMRKGPSGINLQAIYRSGFKCGQCNKTYSSKDEYL 122

Query: 121 DLTVTSGMKEYVEVKPGGTELFRSPLVSFLYERGWRQNFNRSGFPGPDEEFKMAMEYFKS 180
           DLTVT+ + +Y EVKP  TELFRSPLVSFLYERGWRQ F RSGFPGPDEEF+MA EYFK 
Sbjct: 123 DLTVTADLDDYNEVKPITTELFRSPLVSFLYERGWRQAFKRSGFPGPDEEFRMAEEYFKE 182

Query: 181 VEGGLLVDASCGSGLFSRKFAKSGSFSGVIALDFSESMLLQCYDFIKKDAALLNS-NLAL 240
            EGGLLVD SCGSGLFSRKFA+SG +SGVIALD+SE+ML QC +FIK D    NS N+A+
Sbjct: 183 AEGGLLVDVSCGSGLFSRKFAQSGKYSGVIALDYSENMLRQCKEFIKNDNTFDNSTNIAV 242

Query: 241 VRADISRLPFSSGSVDAVHAGAALHCWPSPSNAIAEITRIMRSGGVFVGTTFLRYTSSTP 300
           VRAD+SRLPF SGSVDAVHAGAALHCWPSP+NAIAEI R++RSGGVFVGTTFLRY+ STP
Sbjct: 243 VRADVSRLPFPSGSVDAVHAGAALHCWPSPTNAIAEICRVLRSGGVFVGTTFLRYSPSTP 302

Query: 301 WYIRFSRQRAFQNFGYLTEEEIEDLCKSCGLINYSSKVQQSFIMFSAQKP 340
           W IR  + R  Q++ YL ++EI+D+C SCGL +Y   +Q SFIMF+A+KP
Sbjct: 303 WIIRPFQSRILQSYNYLMQDEIKDVCTSCGLTDYEDYIQDSFIMFTARKP 352

BLAST of Lcy08g016860 vs. TAIR 10
Match: AT1G78140.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 297.0 bits (759), Expect = 2.0e-80
Identity = 158/315 (50.16%), Postives = 203/315 (64.44%), Query Frame = 0

Query: 32  STHRRSFSSTVRASSTLVIESDLGIQQDQNSKIDVFSCPVCYEPLL-RKGPPGFNLPAIY 91
           S H  S S +V  +S   ++  +  +     +  + +CP+CY  L     P G    A  
Sbjct: 41  SAHASSASVSVETNSNSNVDFVIEKKDKNRGEKKILACPICYNSLAWISQPNGLIESAAS 100

Query: 92  RSGFKCRRCNKSYTSRNTFLDLTVTSGMKEYVEVKPGGTELFRSPLVSFLYERGWRQNFN 151
               +C  C +SY+   T LDL V SG K Y E  P  TELFR+PLVSFLYERGWRQNF 
Sbjct: 101 GIQVQCNTCKRSYSGNETHLDLAVASGSKRYSEPMPLSTELFRTPLVSFLYERGWRQNFI 160

Query: 152 RSGFPGPDEEFKMAMEYFKSVEGGLLVDASCGSGLFSRKFAKSGSFSGVIALDFSESMLL 211
             GFPGP++EF+MA  Y K V GG ++DASCGSG+FSR F +S  FS VIALD+SE+ML 
Sbjct: 161 WGGFPGPEKEFEMAKAYLKPVLGGNIIDASCGSGMFSRLFTRSDLFSLVIALDYSENMLR 220

Query: 212 QCYDFIKKDAALLN-SNLALVRADISRLPFSSGSVDAVHAGAALHCWPSPSNAIAEITRI 271
           QCY+ + K+    N   L LVRADI+RLPF SGSVDAVHAGAALHCWPSPS+A+AEI+R+
Sbjct: 221 QCYELLNKEENFPNKEKLVLVRADIARLPFLSGSVDAVHAGAALHCWPSPSSAVAEISRV 280

Query: 272 MRSGGVFVGTTFLRYTSSTPW--YIRFSRQRAFQNFG---YLTEEEIEDLCKSCGLINYS 331
           +R GGVFV TTF+ Y     +  +++  RQ   +  G   +L E E+ED+CK+CGL+N++
Sbjct: 281 LRPGGVFVATTFI-YDGPFSFIPFLKNLRQEIMRYSGSHIFLNERELEDICKACGLVNFT 340

Query: 332 SKVQQSFIMFSAQKP 340
                 FIM SA KP
Sbjct: 341 RVRNGPFIMLSATKP 354

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0WPT72.3e-11862.57Uncharacterized methyltransferase At2g41040, chloroplastic OS=Arabidopsis thalia... [more]
Q8LBV42.8e-7950.16Uncharacterized methyltransferase At1g78140, chloroplastic OS=Arabidopsis thalia... [more]
O143211.5e-0527.81Sterol 24-C-methyltransferase erg6 OS=Schizosaccharomyces pombe (strain 972 / AT... [more]
Q5ZT343.4e-0530.48Malonyl-[acyl-carrier protein] O-methyltransferase OS=Legionella pneumophila sub... [more]
Q5HP743.4e-0532.48Demethylmenaquinone methyltransferase OS=Staphylococcus epidermidis (strain ATCC... [more]
Match NameE-valueIdentityDescription
A0A6J1CMF31.2e-17893.22uncharacterized methyltransferase At2g41040, chloroplastic OS=Momordica charanti... [more]
A0A6J1HLD03.0e-17791.45uncharacterized methyltransferase At2g41040, chloroplastic OS=Cucurbita maxima O... [more]
A0A1S3C4D96.2e-17590.86uncharacterized methyltransferase At2g41040, chloroplastic isoform X2 OS=Cucumis... [more]
A0A6J1FCL51.1e-17490.27uncharacterized methyltransferase At2g41040, chloroplastic OS=Cucurbita moschata... [more]
A0A5A7VAW16.9e-17490.88Putative methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
Match NameE-valueIdentityDescription
XP_038889838.12.7e-18094.10uncharacterized methyltransferase At2g41040, chloroplastic [Benincasa hispida][more]
XP_022142970.12.5e-17893.22uncharacterized methyltransferase At2g41040, chloroplastic [Momordica charantia][more]
XP_023536953.12.8e-17791.45uncharacterized methyltransferase At2g41040, chloroplastic [Cucurbita pepo subsp... [more]
XP_022965827.16.2e-17791.45uncharacterized methyltransferase At2g41040, chloroplastic [Cucurbita maxima][more]
XP_008456635.11.3e-17490.86PREDICTED: uncharacterized methyltransferase At2g41040, chloroplastic isoform X2... [more]
Match NameE-valueIdentityDescription
AT2G41040.11.6e-11962.57S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G78140.12.0e-8050.16S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 132..338
e-value: 1.3E-29
score: 105.2
NoneNo IPR availablePANTHERPTHR43591:SF66SUBFAMILY NOT NAMEDcoord: 17..338
NoneNo IPR availablePANTHERPTHR43591METHYLTRANSFERASEcoord: 17..338
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 176..282
e-value: 1.28786E-11
score: 58.5954
IPR013216Methyltransferase type 11PFAMPF08241Methyltransf_11coord: 178..277
e-value: 2.2E-17
score: 63.5
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 141..338

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy08g016860.1Lcy08g016860.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0006744 ubiquinone biosynthetic process
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0050661 NADP binding
molecular_function GO:0004499 N,N-dimethylaniline monooxygenase activity