Lcy08g016420 (gene) Sponge gourd (P93075) v1

BLAST of Lcy08g016420 vs. ExPASy Swiss-Prot
Match: Q9SZ66 (Disease resistance-like protein DSC1 OS=Arabidopsis thaliana OX=3702 GN=DSC1 PE=1 SV=1)

HSP 1 Score: 546.2 bits (1406), Expect = 8.3e-154
Identity = 402/1193 (33.70%), Postives = 602/1193 (50.46%), Query Frame = 0

Query: 6    SSQPISIWIQPQYDVFVSFRGEDVRNNFVSHLQAALKRKGITTFSDEDQLKRGEEISPAL 65
            SS P S     ++DVF+SFRG D RNNF  HLQ AL+ +GI +F D D+L+RG+ ++ AL
Sbjct: 3    SSSPSS----AEFDVFLSFRGFDTRNNFTGHLQKALRLRGIDSFID-DRLRRGDNLT-AL 62

Query: 66   VEAIQQSSISVVVFSQNYASSKWCLEELSKIVERRDMDGQMVMPVFYHVHPSHVRNQTGS 125
             + I++S I+++VFS NYA+S WCL EL KI+E R+ + Q+V+P+FY V  S V  Q  S
Sbjct: 63   FDRIEKSKIAIIVFSTNYANSAWCLRELVKILECRNSNQQLVVPIFYKVDKSDVEKQRNS 122

Query: 126  YAEAFRKHEETLED-KKDRIQTWKDALKQIANLSGFHSANWRL-ESQMIDKITQEILKKL 185
            +A  F+  E T      + I +WK AL   +N+ G+        E++++D+I  +  KKL
Sbjct: 123  FAVPFKLPELTFPGVTPEEISSWKAALASASNILGYVVKEISTSEAKLVDEIAVDTFKKL 182

Query: 186  DRLTLDDVDGLVGIEHRVERIESSLC-TDSNDVRILGIWGMGGIGKTTVARTLFHKIKNQ 245
            + L     +GLVGIE R++ +E  L   D + V I+GI GM GIGKTT+A  L+ +++ Q
Sbjct: 183  NDLAPSGNEGLVGIESRLKNLEKLLSWEDLDTVHIIGIVGMVGIGKTTLADCLYGRMRGQ 242

Query: 246  FDGCCFLENIRENSGKNGHGLPDLRKKLLSQVMEEAHLPMDI---SREFLKKRMCGKKVL 305
            FDG CFL NIRENSG++  GL  L +KL S V+ +  L +     + E  ++R+  K++L
Sbjct: 243  FDGSCFLTNIRENSGRS--GLESLLQKLFSTVLNDRDLEIGAPGNAHERFERRLKSKRLL 302

Query: 306  IILDDIDDMEQLENLAGRHNWFGVGSRIVITTRNRQLLTEVDEVYDYAKEQLTYEEALLL 365
            I+LDD++D +Q+  L G   W+  GSRI+ITTR+ +L+ E  +   Y   +L   EAL L
Sbjct: 303  IVLDDVNDEKQIRYLMGHCKWYQGGSRIIITTRDSKLI-ETIKGRKYVLPKLNDREALKL 362

Query: 366  FSHHAFNSSHPTEAYLELSRQAINYACGIPLALKILGSCLRTKDEKTWESALARLERFSN 425
            FS +AF++S P + +  L+   ++YA G PLALK+LGS L  +D+  WE+ L RL+  S+
Sbjct: 363  FSLNAFSNSFPLKEFEGLTNMVLDYAKGHPLALKVLGSDLCERDDLYWEAKLDRLKSRSH 422

Query: 426  DKIVKRVLEISFEGLDWSEKNILLDVACFFKGEDVKFATSIWNVCGFFPDYGISVLRDKC 485
              I + VLE S+E L   +KN+ LD+ACFF+ E+V + TS+ N  G      +  L DKC
Sbjct: 423  GDIYE-VLETSYEELTTEQKNVFLDIACFFRSENVDYVTSLLNSHGVDVSGVVKDLVDKC 482

Query: 486  LISLLCNSQLWMHDLIQEMCKAIVRKENTDPGKD-------------------------- 545
            LI+ L ++++ MHD++Q M K I  K  T   +D                          
Sbjct: 483  LIT-LSDNRIEMHDMLQTMAKEISLKVETIGIRDCRWLSRHGNQCQWHIRLWDSEDICDL 542

Query: 546  ----------------------------AFVKMKNLRLLKFFSSK----LDASMRIRLPR 605
                                        AF  M NL+ LK + S      +A  ++ L R
Sbjct: 543  LTEGLGTDKIRGIFLDTSKLRAMRLSAKAFQGMYNLKYLKIYDSHCSRGCEAEFKLHLRR 602

Query: 606  GLTMLPDSLRYLFWDGYPNKFLPSKFNAQNLLGLSLRQGCINQLWKEHSMFKHSSICLKH 665
            GL+ LP+ L YL W GYP + +P  F+ +NL+ L L    + ++W +          +  
Sbjct: 603  GLSFLPNELTYLHWHGYPLQSIPLDFDPKNLVDLKLPHSQLEEIWDDEK-------DVGM 662

Query: 666  LKRLDLSGCPNFSNFSAICDNMEHLYELSLEG-TSASNMPSSIGNLTELLSLNLSYCKEL 725
            LK +DLS   N      +  N  +L  L+LEG TS   +PS+I  L +L+ LNL  C  L
Sbjct: 663  LKWVDLSHSINLRQCLGLA-NAHNLERLNLEGCTSLKKLPSTINCLEKLIYLNLRDCTSL 722

Query: 726  VSLPDSICNLKRLQRLDLNGCESLQHLPENIGKLESLWWLRADGTSIKNVPSSIICLKKI 785
             SLP  I   + LQ L L+GC SL+  P     +E    L  DGT IK++P SI   +++
Sbjct: 723  RSLPKGI-KTQSLQTLILSGCSSLKKFPLISENVEV---LLLDGTVIKSLPESIQTFRRL 782

Query: 786  GTLSFSGCRGKGIKRSTSHW---------------------------------------- 845
              L+   C  K +K  +S                                          
Sbjct: 783  ALLNLKNC--KKLKHLSSDLYKLKCLQELILSGCSQLEVFPEIKEDMESLEILLMDDTSI 842

Query: 846  --MSWLLDRSNNHTTSFVGLQ---------LPPLGGIQKLEALYLDNCNLLELP-ALDKL 905
              M  ++  SN  T S  G           +PP  G  +L  LYL  C+L +LP  +  L
Sbjct: 843  TEMPKMMHLSNIKTFSLCGTSSHVSVSMFFMPPTLGCSRLTDLYLSRCSLYKLPDNIGGL 902

Query: 906  PSLESLDLRGNNFENLPQTIFSNLSSLKYLKLSYCRRLRSLPELPPNLRLLKADECTSLE 965
             SL+SL L GNN ENLP++ F+ L++LK+  L +C+ L+SLP LP NL+ L A EC SLE
Sbjct: 903  SSLQSLCLSGNNIENLPES-FNQLNNLKWFDLKFCKMLKSLPVLPQNLQYLDAHECESLE 962

Query: 966  SFMSSVTLYKGIGRWNAKMYQFGNCLKLKD--QCNNILESMIKGIHLSEGIVQNLYDQGY 1025
            +  + +T    +G     M+ F NC KL    Q + +  + IK   ++    +  Y +G+
Sbjct: 963  TLANPLTPLT-VGERIHSMFIFSNCYKLNQDAQASLVGHARIKSQLMANASAKRYY-RGF 1022

Query: 1026 CCRDGLNFSPWVAFPGSEIPEWFSNQAVGKATTLHLPPRCLD-KSLGFALCFILSPNGTF 1061
                 +     + +P +EIP WF +Q +G++  + LPP   D   +G AL  ++S    F
Sbjct: 1023 VPEPLVG----ICYPATEIPSWFCHQRLGRSLEIPLPPHWCDINFVGLALSVVVS----F 1082

BLAST of Lcy08g016420 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.1e-149
Identity = 372/1070 (34.77%), Postives = 541/1070 (50.56%), Query Frame = 0

Query: 1    MAESSSSQPISIWIQPQYDVFVSFRGEDVRNNFVSHLQAALKRKGITTFSDEDQLKRGEE 60
            MA SSSS   S W    YDVF+SFRGED R  F SHL   L  KGI TF D+ +L+ G  
Sbjct: 1    MASSSSS---SRW---SYDVFLSFRGEDTRKTFTSHLYEVLNDKGIKTFQDDKRLEYGAT 60

Query: 61   ISPALVEAIQQSSISVVVFSQNYASSKWCLEELSKIVERRDMDGQMVMPVFYHVHPSHVR 120
            I   L +AI++S  ++VVFS+NYA+S+WCL EL KI+E +    Q V+P+FY V PSHVR
Sbjct: 61   IPGELCKAIEESQFAIVVFSENYATSRWCLNELVKIMECKTRFKQTVIPIFYDVDPSHVR 120

Query: 121  NQTGSYAEAFRKHEETLEDKKDRIQTWKDALKQIANLSGFHSANWRLESQMIDKITQEIL 180
            NQ  S+A+AF +HE   +D  + IQ W+ AL + ANL G      + ++  I +I  +I 
Sbjct: 121  NQKESFAKAFEEHETKYKDDVEGIQRWRIALNEAANLKGSCDNRDKTDADCIRQIVDQIS 180

Query: 181  KKLDRLTLDDVDGLVGIEHRVERIESSLCTDSNDVRILGIWGMGGIGKTTVARTLFHKI- 240
             KL +++L  +  +VGI+  +E+IES L    N VRI+GIWGMGG+GKTT+AR +F  + 
Sbjct: 181  SKLCKISLSYLQNIVGIDTHLEKIESLLEIGINGVRIMGIWGMGGVGKTTIARAIFDTLL 240

Query: 241  -----KNQFDGCCFLENIRENSGKNGHGLPDLRKKLLSQVMEEA--HLPMDISREFLKKR 300
                   QFDG CFL++I+EN      G+  L+  LLS+++ E   +   +  +  +  R
Sbjct: 241  GRMDSSYQFDGACFLKDIKENK----RGMHSLQNALLSELLREKANYNNEEDGKHQMASR 300

Query: 301  MCGKKVLIILDDIDDMEQ-LENLAGRHNWFGVGSRIVITTRNRQLLTEVDEVYDYAKEQL 360
            +  KKVLI+LDDID+ +  LE LAG  +WFG GSRI+ITTR++ L+ + D +Y+     L
Sbjct: 301  LRSKKVLIVLDDIDNKDHYLEYLAGDLDWFGNGSRIIITTRDKHLIEKNDIIYEVT--AL 360

Query: 361  TYEEALLLFSHHAFNSSHPTEAYLELSRQAINYACGIPLALKILGSCLRTKDEKTWESAL 420
               E++ LF  HAF    P E + +LS + +NYA G+PLALK+ GS L       W+SA+
Sbjct: 361  PDHESIQLFKQHAFGKEVPNENFEKLSLEVVNYAKGLPLALKVWGSLLHNLRLTEWKSAI 420

Query: 421  ARLERFSNDKIVKRVLEISFEGLDWSEKNILLDVACFFKGEDVKFATSIWNVCGFFPDYG 480
              ++  S   I+ + L+IS++GL+  ++ + LD+ACF +GE+  +   I   C    +YG
Sbjct: 421  EHMKNNSYSGIIDK-LKISYDGLEPKQQEMFLDIACFLRGEEKDYILQILESCHIGAEYG 480

Query: 481  ISVLRDKCLISLLCNSQLWMHDLIQEMCKAIVRKENTDPG--------KDAFVKMKN--- 540
            + +L DK L+ +   +Q+ MHDLIQ+M K IV  +  DPG        K+    M N   
Sbjct: 481  LRILIDKSLVFISEYNQVQMHDLIQDMGKYIVNFQK-DPGERSRLWLAKEVEEVMSNNTG 540

Query: 541  ------------LRLLKFFSSKLDASMRIRL--------PRGLTMLPDSLRYLFWDGYPN 600
                           L+F +  +    R+R+           +  LP++LR      YP 
Sbjct: 541  TMAMEAIWVSSYSSTLRFSNQAVKNMKRLRVFNMGRSSTHYAIDYLPNNLRCFVCTNYPW 600

Query: 601  KFLPSKFNAQNLLGLSLRQGCINQLWKEHSMF---------------------------- 660
            +  PS F  + L+ L LR   +  LW E                                
Sbjct: 601  ESFPSTFELKMLVHLQLRHNSLRHLWTETKHLPSLRRIDLSWSKRLTRTPDFTGMPNLEY 660

Query: 661  ------------KHSSICLKHLKRLDLSGCPNFSNFSAI------------CDNMEHLYE 720
                         HS  C   +  L L+ C +   F  +            CD++E L E
Sbjct: 661  VNLYQCSNLEEVHHSLGCCSKVIGLYLNDCKSLKRFPCVNVESLEYLGLRSCDSLEKLPE 720

Query: 721  ----------LSLEGTSASNMPSSI----GNLTELLSLNLSYCKELVSLPDSICNLKRLQ 780
                      + ++G+    +PSSI     ++T+LL  N+   K LV+LP SIC LK L 
Sbjct: 721  IYGRMKPEIQIHMQGSGIRELPSSIFQYKTHVTKLLLWNM---KNLVALPSSICRLKSLV 780

Query: 781  RLDLNGCESLQHLPENIGKLESLWWLRADGTSIKNVPSSIICLKKIGTLSFSGCRGKGIK 840
             L ++GC  L+ LPE IG L++L    A  T I   PSSII L K+  L F G +     
Sbjct: 781  SLSVSGCSKLESLPEEIGDLDNLRVFDASDTLILRPPSSIIRLNKLIILMFRGFK----- 840

Query: 841  RSTSHWMSWLLDRSNNHTTSFVGLQLPPLG-GIQKLEALYLDNCNLLE--LP-ALDKLPS 900
                                 V  + PP+  G+  LE L L  CNL++  LP  +  L S
Sbjct: 841  -------------------DGVHFEFPPVAEGLHSLEYLNLSYCNLIDGGLPEEIGSLSS 900

Query: 901  LESLDLRGNNFENLPQTIFSNLSSLKYLKLSYCRRLRSLPELPPNLRLLKADECTSLESF 958
            L+ LDL  NNFE+LP +I + L +L+ L L  C+RL  LPELPP L  L  D   +L+  
Sbjct: 901  LKKLDLSRNNFEHLPSSI-AQLGALQSLDLKDCQRLTQLPELPPELNELHVDCHMALKFI 960

BLAST of Lcy08g016420 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 6.1e-149
Identity = 359/918 (39.11%), Postives = 503/918 (54.79%), Query Frame = 0

Query: 2   AESSSSQPISIWIQPQYDVFVSFRGEDVRNNFVSHLQAALKRKGITTFSDEDQLKRGEEI 61
           A SSSS P SI     YDVF+SFRGED R NF  HL +AL R+GI TF D D+L+RGE I
Sbjct: 9   ASSSSSTP-SIPRTTTYDVFLSFRGEDTRYNFTDHLYSALGRRGIRTFRD-DRLRRGEAI 68

Query: 62  SPALVEAIQQSSISVVVFSQNYASSKWCLEELSKIVERRDMDGQMVMPVFYHVHPSHVRN 121
           +P L++AI++S  SV+VFS+NYA S+WCL+EL KI+E +   G  V P+FYHV PSHVR 
Sbjct: 69  APELLKAIEESRSSVIVFSENYAHSRWCLDELVKIMECQKDLGHAVFPIFYHVDPSHVRK 128

Query: 122 QTGSYAEAFRKHEETLEDKKDRIQTWKDALKQIANLSGFHSANWRLESQMIDKITQEILK 181
           Q GS+ EAF  +E   E+ KD+I  W+ AL + ANLSG+H  + R ES  I +IT  I +
Sbjct: 129 QEGSFGEAFAGYE---ENWKDKIPRWRTALTEAANLSGWHLLDDRYESNQIKEITNSIFR 188

Query: 182 KLDRLTLDDVDGLVGIEHRVERIESSLCTDSNDVRILGIWGMGGIGKTTVARTLFHKIKN 241
           +L    LD    LVGI+  V+ +   L  +S+DVR++GI+G+GGIGKTT+A+ +++++  
Sbjct: 189 QLKCKRLDVGANLVGIDSHVKEMILRLHLESSDVRMVGIYGVGGIGKTTIAKVIYNELSC 248

Query: 242 QFDGCCFLENIRENSGKNGHGLPDLRKKLLSQVMEEAHLPMDIS-----REFLKKRMCGK 301
           +F+   FLENIRE  G N   L  L+ +LL  ++ E     +IS        +K  +  +
Sbjct: 249 EFEYMSFLENIRE--GSNPQVLFHLQNQLLGDIL-EGEGSQNISSVAHRASMIKDILLSR 308

Query: 302 KVLIILDDIDDMEQLENLAGRHNWFGVGSRIVITTRNRQLLT--EVDEVYDYAKEQLTYE 361
           +V I+LDD+DD+ QLE L G   W G GSR++ITTRN+ +L   EVD++Y+   E L +E
Sbjct: 309 RVFIVLDDVDDLSQLEYLLGHREWLGEGSRVIITTRNKHVLAVQEVDDLYEV--EGLNFE 368

Query: 362 EALLLFSHHAFNSSHPTEAYLELSRQAINYACGIPLALKILGSCLRTKDEKTWESALARL 421
           EA  LFS +AF  + P   Y  L+ + + Y  G+PLALK+LGS L  K    WE  L +L
Sbjct: 369 EACELFSLYAFKQNLPKSDYRNLTCRVVGYCQGLPLALKVLGSLLCKKTIPQWEGELKKL 428

Query: 422 ERFSNDKI-VKRVLEISFEGLDWSEKNILLDVACFFKGEDVKFATSIWNVCGFFPDYGIS 481
           +  S  K  + +VL+ S++GLD  +KNI LD+ACFFKGE   F   I + C F  + GIS
Sbjct: 429 D--SEPKADIHKVLKRSYDGLDRIDKNIFLDLACFFKGEGRDFVLRILDGCDFPAETGIS 488

Query: 482 VLRDKCLISLLCNSQLWMHDLIQEMCKAIVRKE-NTDPGK-------------------- 541
            L D CLI+L  N Q+ MHDLIQ+M   IVR+    +P K                    
Sbjct: 489 NLNDLCLITLPYN-QICMHDLIQQMGWEIVRENFPVEPNKWSRLWDPCDFERALTADEGI 548

Query: 542 --------------------DAFVKMKNLRLLKFFSSK--------------------LD 601
                               + F KM  LRLLK +SS                     + 
Sbjct: 549 KSVETMSLDLSKLKRVCSNSNVFAKMTKLRLLKVYSSSDIDSAHGDSDEDIEEVYDVVMK 608

Query: 602 ASMRIRLPRGLTMLPDSLRYLFWDGYPNKFLPSKFNAQNLLGLSLRQGCINQLWKEHSMF 661
            + +++L +        LRYL WDGYP   LP  F+   L+ L L+   I QLW+ H   
Sbjct: 609 DASKMQLGQSFKFPSYELRYLRWDGYPLDSLPLNFDGGKLVELHLKCSNIKQLWQGHK-- 668

Query: 662 KHSSICLKHLKRLDLSGCPNFSNFSAICDNMEHLYELSLEG-TSASNMPSSIGNLTELLS 721
                 L+ LK +DLS     S  S    +M +L  L L G  S  ++  S+GN+ +L +
Sbjct: 669 -----DLERLKVIDLSYSRKLSQMSEF-SSMPNLERLCLSGCVSLIDIHPSVGNMKKLTT 728

Query: 722 LNLSYCKELVSLPDSICNLKRLQRLDLNGCESLQHLPENIGKLESLWWLRADGTSIKNVP 781
           L+L  C +L +LPDSI +L+ L+ L L+ C   +  PE  G ++SL  L    T+IK++P
Sbjct: 729 LSLRSCNKLKNLPDSIGDLESLESLYLSNCSKFEKFPEKGGNMKSLTELDLKNTAIKDLP 788

Query: 782 SSIICLKKIGTLSFSGC--------RGKGIKRSTSHWMSWLLDRSNNHTTSFVGLQLPPL 840
            SI  L+ + +L  S C        +G  +K  T       LD  N   T+   L    +
Sbjct: 789 DSIGDLESLESLYLSNCSKFEKFPEKGGNMKSLTE------LDLKN---TAIKDLP-DSI 848

BLAST of Lcy08g016420 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 4.1e-145
Identity = 356/913 (38.99%), Postives = 499/913 (54.65%), Query Frame = 0

Query: 2   AESSSSQPISIWIQPQYDVFVSFRGEDVRNNFVSHLQAALKRKGITTFSDEDQLKRGEEI 61
           + SSSS   SI     YDVF+SFRGED R NF  HL +AL R+GI TF D D+L+RGE I
Sbjct: 11  SSSSSSSTPSIPRTITYDVFLSFRGEDTRFNFTDHLYSALGRRGIRTFRD-DKLRRGEAI 70

Query: 62  SPALVEAIQQSSISVVVFSQNYASSKWCLEELSKIVE----RRDMDGQMVMPVFYHVHPS 121
           +P L++AI++S  SV+VFS+NYA S+WCL+EL KI+E    ++D  G  V P+FYHV PS
Sbjct: 71  APELLKAIEESRSSVIVFSENYARSRWCLDELVKIMECHKDKKD-PGHAVFPIFYHVDPS 130

Query: 122 HVRNQTGSYAEAFRKHEETLEDKKDRIQTWKDALKQIANLSGFHSANWRLESQMIDKITQ 181
           HVR Q GS+ EAF  + E L   KD+I  W+ AL + ANLSG+   +   ES  I +IT 
Sbjct: 131 HVRKQEGSFGEAFAGYGENL---KDKIPRWRTALTEAANLSGWPLQD-GYESNQIKEITD 190

Query: 182 EILKKLDRLTLDDVDGLVGIEHRVERIESSLCTDSNDVRILGIWGMGGIGKTTVARTLFH 241
            I ++L    LD    LVGI+  V+ +   L  +S+DVR++G++G+GGIGKTT+A+ +++
Sbjct: 191 SIFRRLKCKRLDAGANLVGIDSHVKEMIWRLHMESSDVRMVGMYGVGGIGKTTIAKVIYN 250

Query: 242 KIKNQFDGCCFLENIRENSGKNGHGLPDLRKKLLSQVME----EAHLPMDISREFLKKRM 301
           ++  +F+   FLENIRE    N  G+  L+ +LL  +++    +    +      +K  +
Sbjct: 251 ELSREFEYMSFLENIREKF--NTQGVSPLQNQLLDDILKGEGSQNINSVAHGASMIKDIL 310

Query: 302 CGKKVLIILDDIDDMEQLENLAGRHNWFGVGSRIVITTRNRQLL--TEVDEVYDYAKEQL 361
             K V I+LDD+DD  QLE L     W G GSR++ITTRN+ +L   +VD++Y+   + L
Sbjct: 311 SSKIVFIVLDDVDDQSQLEYLLRHREWLGEGSRVIITTRNKHVLDVQKVDDLYEV--KGL 370

Query: 362 TYEEALLLFSHHAFNSSHPTEAYLELSRQAINYACGIPLALKILGSCLRTKDEKTWESAL 421
            +EEA  LFS +AF  + P   Y  LS + + Y  G+PLALK+LG  L  K    WES L
Sbjct: 371 NFEEACELFSLYAFEQNLPKSDYRNLSHRVVGYCQGLPLALKVLGCLLLKKTIPEWESEL 430

Query: 422 ARLERFSNDKIVKRVLEISFEGLDWSEKNILLDVACFFKGEDVKFATSIWNVCGFFPDYG 481
            +L+R    +I+  VL+ S++GL  +EK+I LDVACFFKGED  F + I + C F  + G
Sbjct: 431 RKLDREPEAEILS-VLKRSYDGLGRTEKSIFLDVACFFKGEDRDFVSKILDACDFHAEIG 490

Query: 482 ISVLRDKCLISLLCNSQLWMHDLIQEMCKAIVRKENTD-PGK------------------ 541
           I  L DKCLI+L  N ++ MHDLIQ+M   IVR++  D P K                  
Sbjct: 491 IKNLNDKCLITLQYN-RIRMHDLIQQMGWEIVREKFPDEPNKWSRLWDTCDFERALTAYK 550

Query: 542 ----------------------DAFVKMKNLRLLKFFSS--------KLDA--------- 601
                                 +AF KM  LRLLK  SS         +DA         
Sbjct: 551 GIKRVETISLDLSKLKRVCSNSNAFAKMTRLRLLKVQSSLDIDFEPEYIDADDKVELYDV 610

Query: 602 ----SMRIRLPRGLTMLPDSLRYLFWDGYPNKFLPSKFNAQNLLGLSLRQGCINQLWKEH 661
               + ++RL RG       LRYL WDGYP  FLPS F+   L+ L L+   I QL    
Sbjct: 611 VMKNASKMRLGRGFKFPSYELRYLRWDGYPLDFLPSNFDGGKLVELHLKCSNIKQL---- 670

Query: 662 SMFKHSSICLKHLKRLDLSGCPNFSNFSAICDNMEHLYELSLEG-TSASNMPSSIGNLTE 721
              +  +  L+ LK +DLS     S  S    +M +L  L L G  S  ++  S+GN+ +
Sbjct: 671 ---RLGNKDLEMLKVIDLSYSRKLSQMSEF-SSMPNLERLFLRGCVSLIDIHPSVGNMKK 730

Query: 722 LLSLNLSYCKELVSLPDSICNLKRLQRLDLNGCESLQHLPENIGKLESLWWLRADGTSIK 781
           L +L+L  CK+L +LPDSI +L+ L+ LDL  C   +  PE  G ++SL  L    T+IK
Sbjct: 731 LTTLSLKSCKKLKNLPDSIGDLESLEILDLAYCSKFEKFPEKGGNMKSLTELDLQNTAIK 790

Query: 782 NVPSSIICLKKIGTLSFSGCRGKGIKRSTSHWMSWLLDRSNNHTTSFVGLQLPPLGGIQK 840
           ++P SI  L+ +  L  S C  K  K          L   +   T+   L    +  ++ 
Sbjct: 791 DLPDSIGDLESLKYLDLSDC-SKFEKFPEKGGNMKSLRELDLRNTAIKDLP-DSIRDLES 850

BLAST of Lcy08g016420 vs. ExPASy Swiss-Prot
Match: F4JT80 (Disease resistance protein RPP2B OS=Arabidopsis thaliana OX=3702 GN=RPP2B PE=1 SV=2)

HSP 1 Score: 480.3 bits (1235), Expect = 5.6e-134
Identity = 361/1073 (33.64%), Postives = 536/1073 (49.95%), Query Frame = 0

Query: 1    MAESSSSQPISIWIQPQYDVFVSFRGEDVRNNFVSHLQAALKRKGITTFSDEDQLKRGEE 60
            MA +SSS  I +  + ++DVFVSFRG D R++F SHL   L+ KGI  FSD  +L+ GE 
Sbjct: 1    MAFASSSSSI-VLSKCEFDVFVSFRGADTRHDFTSHLVKYLRGKGIDVFSDA-KLRGGEY 60

Query: 61   ISPALVEAIQQSSISVVVFSQNYASSKWCLEELSKIVERRDMDGQMVMPVFYHVHPSHVR 120
            IS  L + I+QS +S+VVFS++YA+S WCLEE+ KI++RR      V+P+FY V  S V 
Sbjct: 61   IS-LLFDRIEQSKMSIVVFSEDYANSWWCLEEVGKIMQRRKEFNHGVLPIFYKVSKSDVS 120

Query: 121  NQTGSYAEAFRKHEETLEDKKDRIQTWKDALKQIANLSGFHSANWRLESQMIDKITQEIL 180
            NQTGS+   F+   +     + +I+  K ALK  +N+ GF       E   +D+I +   
Sbjct: 121  NQTGSFEAVFQSPTKIFNGDEQKIEELKVALKTASNIRGFVYPENSSEPDFLDEIVKNTF 180

Query: 181  KKLDRLTLDDV-DGLVGIEHRVERIESSLCTDSND-VRILGIWGMGGIGKTTVARTLFHK 240
            + L+ L+   + D L GIE R + +E  L  D+++ VR++G+ GM GIGKTTVA  ++ +
Sbjct: 181  RMLNELSPCVIPDDLPGIESRSKELEKLLMFDNDECVRVVGVLGMTGIGKTTVADIVYKQ 240

Query: 241  IKNQFDGCCFLENIRENSGKNGHGLPDLRKKLLSQVMEEAHLPMDIS---REFLKKRMCG 300
               +FDG  FLE+I +NS +  +GLP L +KLL ++++  ++ +        FL+     
Sbjct: 241  NFQRFDGYEFLEDIEDNSKR--YGLPYLYQKLLHKLLDGENVDVRAQGRPENFLR----N 300

Query: 301  KKVLIILDDIDDMEQLENLAGRHNWFGVGSRIVITTRNRQLLTEVDEVYDYAKEQLTYEE 360
            KK+ I+LD++ + +Q+E L G+ N +  GSRIVI TR+++LL +  +   Y   +L   E
Sbjct: 301  KKLFIVLDNVTEEKQIEYLIGKKNVYRQGSRIVIITRDKKLLQKNADA-TYVVPRLNDRE 360

Query: 361  ALLLFSHHAFNSSHPTEAYLELSRQAINYACGIPLALKILGSCLRTKDEKTWESALARLE 420
            A+ LF    F + +PTE +++LS   + YA G+PLALK+LG  L T D   W+  L  L+
Sbjct: 361  AMELFCLQVFGNHYPTEEFVDLSNDFVCYAKGLPLALKLLGKGLLTHDINYWKKKLEFLQ 420

Query: 421  RFSNDKIVKRVLEISFEGLDWSEKNILLDVACFFKGEDVKFATSIWNVCGFFPDYGISVL 480
              + DK +++ L+ S++ LD  +K++ LD+ACFF+ E   F +SI           +  L
Sbjct: 421  -VNPDKELQKELKSSYKALDDDQKSVFLDIACFFRSEKADFVSSILKSDDIDAKDVMREL 480

Query: 481  RDKCLISLLCNSQLWMHDLIQEMCKAIVRKENTDPG---------KD------------- 540
             +KCL++ +   ++ MHDL+  M K I ++++             KD             
Sbjct: 481  EEKCLVT-ISYDRIEMHDLLHAMGKEIGKEKSIRKAGERRRLWNHKDIRDILEHNTGTEC 540

Query: 541  -------------------AFVKMKNLRLLKFFSSK----LDASMRIRLPRGLTMLPDSL 600
                               AF  +  L+ LKF SS      D     +  +     PD L
Sbjct: 541  VRGIFLNMSEVRRIKLFPAAFTMLSKLKFLKFHSSHCSQWCDNDHIFQCSKVPDHFPDEL 600

Query: 601  RYLFWDGYPNKFLPSKFNAQNLLGLSLRQGCINQLWKEH---------------SMFKHS 660
             YL W GYP   LPS F+ + L+ LSLR   I QLW++                 +   S
Sbjct: 601  VYLHWQGYPYDCLPSDFDPKELVDLSLRYSHIKQLWEDEKNTESLRWVDLGQSKDLLNLS 660

Query: 661  SIC-LKHLKRLDLSGCPNFSNFSAI-------------CDNMEHL--------------- 720
             +   K+L+RLDL GC +     ++             C ++E L               
Sbjct: 661  GLSRAKNLERLDLEGCTSLDLLGSVKQMNELIYLNLRDCTSLESLPKGFKIKSLKTLILS 720

Query: 721  ---------------YELSLEGTSASNMPSSIGNLTELLSLNLSYCKELVSLPDSICNLK 780
                             L LEGT+   +   I +L  L+ LNL  C++L  LP+ +  LK
Sbjct: 721  GCLKLKDFHIISESIESLHLEGTAIERVVEHIESLHSLILLNLKNCEKLKYLPNDLYKLK 780

Query: 781  RLQRLDLNGCESLQHLPENIGKLESLWWLRADGTSIKNVPSSIICLKKIGTLSFSGCRGK 840
             LQ L L+GC +L+ LP    K+E L  L  DGTSIK  P  + CL  +   SF  CR  
Sbjct: 781  SLQELVLSGCSALESLPPIKEKMECLEILLMDGTSIKQTP-EMSCLSNLKICSF--CRP- 840

Query: 841  GIKRSTSHWMSWLLDRSNNHTTSFVGLQLPPLGGIQKLEALYLDNCNLLELP-ALDKLPS 900
                        ++D S        GL + P  G   L  LYL NCN+ +LP     L S
Sbjct: 841  ------------VIDDS-------TGLVVLPFSGNSFLSDLYLTNCNIDKLPDKFSSLRS 900

Query: 901  LESLDLRGNNFENLPQTIFSNLSSLKYLKLSYCRRLRSLPELPPNLRLLKADECTSLESF 960
            L  L L  NN E LP++I   L SL  L L +C RL+SLP LP NL+ L A  C SLE+ 
Sbjct: 901  LRCLCLSRNNIETLPESI-EKLYSLLLLDLKHCCRLKSLPLLPSNLQYLDAHGCGSLENV 960

BLAST of Lcy08g016420 vs. ExPASy TrEMBL
Match: A0A6J1DE10 (TMV resistance protein N-like OS=Momordica charantia OX=3673 GN=LOC111020196 PE=4 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 1.9e-209
Identity = 421/793 (53.09%), Postives = 515/793 (64.94%), Query Frame = 0

Query: 168 ESQMIDKITQEILKKLDRLTLDDV-DGLVGIEHRVERIESSLCTDSNDVRILGIWGMGGI 227
           E+  I++I+ EILKKL+RLT  DV +GLVGIE R+ERIES +   S DV ILGIWGMGGI
Sbjct: 29  EATFIEEISGEILKKLNRLTPIDVHEGLVGIEARIERIESRVRVGSRDVCILGIWGMGGI 88

Query: 228 GKTTVARTLFHKIKNQFDGCCFLENIRENSGKNGHGLPDLRKKLLSQVMEEA--HLPMDI 287
           GKTT+AR LF +IK+QFDGCCFL+N+REN  K  HGL  L++KLL +V+EE    L  +I
Sbjct: 89  GKTTLARALFDRIKSQFDGCCFLKNVRENQIK--HGLSHLQEKLLCEVLEERRDQLIGNI 148

Query: 288 SREFLKKRMCGKKVLIILDDIDDMEQLENLAGRHNWFGVGSRIVITTRNRQLLTEVDEVY 347
           SR FLKKRM G+KVLI+LDD++DMEQLE+LAG  +WFG GSRI++TTR+RQ++   DEVY
Sbjct: 149 SRGFLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIVTTRHRQVVNGADEVY 208

Query: 348 DYAKEQLTYEEALLLFSHHAF---NSSHPTEAYLELSRQAINYACGIPLALKILGSCLRT 407
           +   + L++EEA+ LFSHHAF      H  E +LELS +A+NYA GIPLALK+LGSCL  
Sbjct: 209 E--AQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAGGIPLALKVLGSCLCG 268

Query: 408 KDEKTWESALARLERFSNDKIVKRVLEISFEGLDWSEKNILLDVACFFKGEDVKFATSIW 467
           KD+  WES LA+LER  N+K V+ VL++SFEGLD +EK I LDVACFFKG+ V  A ++W
Sbjct: 269 KDKPVWESKLAKLERLPNEK-VQNVLKVSFEGLDRTEKGIFLDVACFFKGDHVGIAKAMW 328

Query: 468 NVCGFFPDYGISVLRDKCLISLLCNSQLWMHDLIQEMCKAIVRKEN-TDPGK-------- 527
           + CGFFP+ G+SVL DKCL+S+  NS   MHDLI+EM K IVR+E+  +PGK        
Sbjct: 329 DACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQEDMEEPGKRSRGTNAI 388

Query: 528 -----------------DAFVKMKNLRLLKFFSSKLDAS--MRIRLPRGLTMLPDSLRYL 587
                            DAF +M  LR L FFS  L  S   RIRLP GLTMLPDSL YL
Sbjct: 389 KGILLDLSKIKEVELGEDAFAEMTKLRFLNFFSKNLGESTKTRIRLPHGLTMLPDSLMYL 448

Query: 588 FWDGYPNKFLPSKFNAQNLLGLSLRQGCINQLWKEHSMF---KH---------------- 647
            WDGYP KF PS F+   L  L LR  CI QL K   +F   KH                
Sbjct: 449 SWDGYPYKFFPSNFDPDKLFWLRLRHSCIKQLSKGQKIFTNLKHINLSDSEYLSEVPDVS 508

Query: 648 --------------------------------------------SSICLKHLKRLDLSGC 707
                                                       SSICLK L+ LDLSGC
Sbjct: 509 EAPYLEDINFAGCIQLCTIHPSMATLSKLKTLNLRDCRKLRNLPSSICLKSLQELDLSGC 568

Query: 708 PNFSNFSAICDNMEHLYELSLEGTSASNMPSSIGNLTELLSLNLSYCKELVSLPDSICNL 767
            +  NF  I D MEHL+ LSL GT+ SN+PSSI N   L+ L+L+ CK LVSLPDSICNL
Sbjct: 569 SSLQNFPTISDYMEHLWSLSLSGTAISNLPSSIENFYGLVGLDLTNCKNLVSLPDSICNL 628

Query: 768 KRLQRLDLNGCESLQHLPENIGKLESLWWLRADGTSIKNVPSSIICLKKIGTLSFSGCRG 827
           K L  L L+ CE+LQHLPENIG LESL  +  D TSIK VPSSI+CLK I  LSF+GCRG
Sbjct: 629 KCLDSLILSSCENLQHLPENIGNLESLSTVYVDETSIKQVPSSIVCLKNIFELSFNGCRG 688

Query: 828 KGIKRSTSHWMSWLLDRSNNHTTSFVGLQLPPLGGIQKLEALYLDNCNLLELPA-LDKLP 863
                S   ++SW L RS + ++  +GLQLPPLGG ++L +L L NCNL+E P+ L  LP
Sbjct: 689 TETLSSLG-FLSWFLPRSRSSSSCNMGLQLPPLGGFKQLSSLSLSNCNLVEAPSGLHNLP 748

BLAST of Lcy08g016420 vs. ExPASy TrEMBL
Match: A0A438BVS2 (Disease resistance-like protein DSC1 OS=Vitis vinifera OX=29760 GN=DSC1_77 PE=4 SV=1)

HSP 1 Score: 707.2 bits (1824), Expect = 1.0e-199
Identity = 474/1221 (38.82%), Postives = 661/1221 (54.14%), Query Frame = 0

Query: 1    MAESSSSQPISIWIQPQYDVFVSFRGEDVRNNFVSHLQAALKRKGITTFSDEDQLKRGEE 60
            MA SSSS   S   Q +YDVF+SFRGED R +F  HL  AL +KGI TF D DQL+RGE+
Sbjct: 1    MAPSSSSS--SSTHQWKYDVFLSFRGEDTRKSFTDHLHTALCQKGINTFMD-DQLRRGEQ 60

Query: 61   ISPALVEAIQQSSISVVVFSQNYASSKWCLEELSKIVERRDMDGQMVMPVFYHVHPSHVR 120
            I PAL+ AI++S  S+++FS NYASS WCL+EL KI++   + G   +PVFY+V+PSHV+
Sbjct: 61   IFPALLNAIEESRFSIIIFSDNYASSSWCLDELVKILDCIKVMGHRALPVFYNVNPSHVK 120

Query: 121  NQTGSYAEAFRKHEETLEDKKDRIQTWKDALKQIANLSGFHSANWRLESQMIDKITQEIL 180
             QTGS+AEAF KHE+   +K +++  W+ AL ++A +SG+ S + R ES++I +I ++IL
Sbjct: 121  KQTGSFAEAFAKHEQENREKMEKVVKWRKALTEVATISGWDSRD-RHESKLIGEIVRDIL 180

Query: 181  KKLDRLTLDDVDGLVGIEHRVERIESSLCTDSNDVRILGIWGMGGIGKTTVARTLFHKIK 240
             KL   +   + GLVG+E R+E ++S LC  S DVR++GIWGM GIGKTT+A+ ++ +I 
Sbjct: 181  NKLVGTSPSYMKGLVGMESRLEAMDSLLCIGSLDVRMVGIWGMAGIGKTTIAKVIYEQIY 240

Query: 241  NQFDGCCFLENIRENSGKNGHGLPDLRKKLLSQVMEEAHLPMDISRE---FLKKRMCGKK 300
             QF+GCCFL N+RE S K  HGLP L+ +LLSQ+++E +  + +  +   F+K  +  +K
Sbjct: 241  TQFEGCCFLSNVREESYK--HGLPYLQMELLSQILKERNPNVGLFNKGINFMKDVLHSRK 300

Query: 301  VLIILDDIDDMEQLENLAGRHNWFGVGSRIVITTRNRQLLT--EVDEVYDYAKEQLTYEE 360
            VLIILDD+D  +QLE+LAG +NWFG GSRI+ITTR+R LLT  EVD +Y+   ++L  +E
Sbjct: 301  VLIILDDVDQRQQLEDLAGDNNWFGSGSRIIITTRDRHLLTCQEVDAIYEV--KELDNDE 360

Query: 361  ALLLFSHHAFNSSHPTEAYLELSRQAINYACGIPLALKILGSCLRTKDEKTWESALARLE 420
            AL LF  +AF   H TE + +L   A++Y  G+PLALK+LGS L TK    W+S L +L+
Sbjct: 361  ALKLFCLYAFRHKHGTEDFRQLCGHALDYTSGLPLALKVLGSSLYTKGIHEWKSELDKLK 420

Query: 421  RFSNDKIVKRVLEISFEGLDWSEKNILLDVACFFKGEDVKFATSIWNVCGFFPDYGISVL 480
            +F N K V+ VL+ SFEGLD +E+NI LD+A F+KG D  F   I + CGFF   GI  L
Sbjct: 421  QFPN-KEVQNVLKTSFEGLDDNEQNIFLDIAFFYKGHDKDFVGDILDSCGFFFGIGIRNL 480

Query: 481  RDKCLISLLCNSQLWMHDLIQEMCKAIVRKENTDPGK----------------------- 540
             DK LI+ +  ++L MHDL+QEM   IVR+++   GK                       
Sbjct: 481  EDKSLIT-ISENKLCMHDLLQEMGWEIVRQKSEVLGKRSRLRVHEDINHVLTTNTGTEAV 540

Query: 541  -----------------DAFVKMKNLRLLKFFSSKLDASM-------------------- 600
                             DAF KMK LRLLK  + ++D S+                    
Sbjct: 541  EGIFLDLSASKELNFSIDAFAKMKRLRLLKICNVQIDRSLGSLSEKELIAYTHDGFEKLK 600

Query: 601  --RIRLPRGLTMLPD-----SLRYLFWDG-------YPN--------------------- 660
              ++   + LT  PD     +LR L   G       +P+                     
Sbjct: 601  SIKLSHSQHLTKTPDFSGMPNLRRLILKGCTSLVEVHPSIGALKKLMFLNLEGCKKLKSF 660

Query: 661  ---------------------KFLPSKFNAQNLLGLSLRQGCINQL-------------- 720
                                 KF   + N ++L  LSL    I  L              
Sbjct: 661  SSSIHMESLQILTLSGCSKLKKFPEVQGNMEHLPNLSLEGTAIKGLPLSFENLTGLALLN 720

Query: 721  ---WKEHSMFKHSSICLKHLKRLDLSGCPNFSNFSAICDNMEHLYELSLEGTSASNMPSS 780
                K       S   LK LK L LS C        I +NME L EL L+G+  + +PSS
Sbjct: 721  LKECKSLESLPRSIFKLKSLKTLILSNCTRLKKLPEIQENMESLMELFLDGSGITELPSS 780

Query: 781  IGNLTELLSLNLSYCKELVSLPDSICNLKRLQRLDLNGCESLQHLPENIGKLESLWWLRA 840
            IG L  L+ LNL  CK+L SLP SIC L  L  L L GC  L+ LP+++G L+ L  L A
Sbjct: 781  IGCLNRLVLLNLKNCKKLASLPQSICELTSLGTLTLCGCSELKELPDDLGSLQCLAELNA 840

Query: 841  DGTSIKNVPSSIICLKKIGTLSFSGCRGKGIKRSTSHWMSWLLDRSNNHTTSFVGLQLPP 900
            DG+ I+ VP SI  L  +  LS +GC+G G  +S +   S+       H++   GL+LP 
Sbjct: 841  DGSGIQEVPPSITLLTNLQELSLAGCKG-GESKSRNMVFSF-------HSSPTEGLRLPS 900

Query: 901  LGGIQKLEALYLDNCNLLE--LPA-LDKLPSLESLDLRGNNFENLPQTIFSNLSSLKYLK 960
            L G+  L+ L L  CNL E  LP+ L  LPSLE LDL  N+F  +P ++ S LS L+ LK
Sbjct: 901  LSGLYSLKVLILQRCNLSEGALPSDLGSLPSLERLDLSRNSFITIPASL-SGLSRLQSLK 960

Query: 961  LSYCRRLRSLPELPPNLRLLKADECTSLESFMSSVTLYKGIGRWNAKMYQFGNCLKL-KD 1020
            L YC+ L+SLPELP +++ L A  CTSLE+F  S + Y          + F NC +L ++
Sbjct: 961  LEYCKSLQSLPELPSSIQRLNAHSCTSLENFSCSSSAYTS-KMMGGLRFNFTNCFRLGEN 1020

Query: 1021 QCNNILESMIKGIHLSEGIVQNLY-DQGYCCRDGLNFSPWVAFPGSEIPEWFSNQAVGKA 1065
            Q ++I+ ++++GI L   I + L  D+G               PGS+IPEWF +Q+VG +
Sbjct: 1021 QGSDIVGAILEGIQLMSSIPKFLVSDRGIPTPQN---EYKACIPGSKIPEWFRHQSVGCS 1080

BLAST of Lcy08g016420 vs. ExPASy TrEMBL
Match: A5B6G5 (TIR domain-containing protein OS=Vitis vinifera OX=29760 GN=VITISV_025072 PE=4 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 6.3e-197
Identity = 442/1171 (37.75%), Postives = 640/1171 (54.65%), Query Frame = 0

Query: 17   QYDVFVSFRGEDVRNNFVSHLQAALKRKGITTFSDEDQLKRGEEISPALVEAIQQSSISV 76
            +YD F+SFRGED R NF +HL AAL +KGI TF D + L RGE+IS  L++AI++S  S+
Sbjct: 21   KYDAFLSFRGEDTRKNFTTHLHAALCQKGINTFKD-NLLLRGEKISAGLLQAIEESRFSI 80

Query: 77   VVFSQNYASSKWCLEELSKIVERRDMDGQMVMPVFYHVHPSHVRNQTGSYAEAFRKHEET 136
            ++FS+NYASS WCL+EL+KI+E  +  G   +PVFY+V PSHVR Q G +A+AF +HE+ 
Sbjct: 81   IIFSENYASSSWCLDELTKILECVEEGGHTALPVFYNVDPSHVRKQKGCFADAFAEHEQV 140

Query: 137  LEDKKDRIQTWKDALKQIANLSGFHSANWRLESQMIDKITQEILKKLDRLTLDDVDGLVG 196
              +K +++  W+ AL ++A +SG+ S + R ES++I++I   IL +       +VD LVG
Sbjct: 141  YREKMEKVVKWRKALTEVATISGWDSRD-RDESEVIEEIVTRILNEPIDAFSSNVDALVG 200

Query: 197  IEHRVERIESSLCTDSNDVRILGIWGMGGIGKTTVARTLFHKIKNQFDGCCFLENIRENS 256
            ++ R+E + S LC  SNDVR +GIWGM GIGKTT+A  ++ +I  +FDGCCFL+++RE+S
Sbjct: 201  MDSRMEDLLSLLCIGSNDVRFVGIWGMAGIGKTTIAEAIYDRIYTKFDGCCFLKDVREDS 260

Query: 257  GKNGHGLPDLRKKLLSQVMEEAHLPMDISREFLKKRMCGKKVLIILDDIDDMEQLENLAG 316
             +  HGL  L++ LLS+V+   +  ++    F+K R+  KKVLI+LD++   ++LE L G
Sbjct: 261  QR--HGLTYLQETLLSRVLGGIN-NLNRGINFIKARLHSKKVLIVLDNVVHRQELEALVG 320

Query: 317  RHNWFGVGSRIVITTRNRQLLTEVDEVYDYAKEQLTYEEALLLFSHHAFNSSHPTEAYLE 376
             H+WFG GSRI+ITTR ++LL E +    Y  E+L Y+EAL LF  +AF   HPTE +++
Sbjct: 321  SHDWFGPGSRIIITTREKRLLIEQEMDAIYEVEKLEYDEALKLFCQYAFRYKHPTEDFMQ 380

Query: 377  LSRQAINYACGIPLALKILGSCLRTKDEKTWESALARLERFSNDKIVKRVLEISFEGLDW 436
            L   A++Y   +PLALK+LGSCL  K    W+S L +  +F N K V  VL+ SF+GLD 
Sbjct: 381  LCHHAVDYTGSLPLALKVLGSCLYRKSIHEWKSELDKFNQFPN-KEVLNVLKTSFDGLDD 440

Query: 437  SEKNILLDVACFFKGEDVKFATSIWNVCGFFPDYGISVLRDKCLISLLCNSQLWMHDLIQ 496
            +EKN+ LD+A F+KGED  F   + +   FFP   I  L DK LI+ + +++L+MHDL+Q
Sbjct: 441  NEKNMFLDIAFFYKGEDKDFVIEVLD--NFFPVSEIGNLVDKSLIT-ISDNKLYMHDLLQ 500

Query: 497  EMCKAIVRKEN-TDPGK----------------------------------------DAF 556
            EM   IVR+E+  DPGK                                        DAF
Sbjct: 501  EMGWEIVRQESIKDPGKRSRLRVHEDIHDVLTTNKGTEAVEGMVFDLSASKELNLSVDAF 560

Query: 557  VKMKNLRLLKFFSSKLDAS-----------------------------MRIRLPRGLTML 616
             KM  LRLL+F++ +   S                              ++ L R     
Sbjct: 561  AKMNKLRLLRFYNCQFYGSSEYLSEKELIASTHDARRWMGYDNSPYNDSKLHLSRDFKFP 620

Query: 617  PDSLRYLFWDGYPNKFLPSKFNAQNLLGLSLRQGCINQLWKEHSMFK------------- 676
             ++LR L W GYP K LPS F+ + L+ L++    + QLW+    F+             
Sbjct: 621  SNNLRSLHWHGYPLKSLPSNFHPEKLVELNMCYSLLKQLWEGKKAFEKLKFIKLSHSQHL 680

Query: 677  --------------------------HSSI-CLKHLKRLDLSGCPNFSNFSAICD-NMEH 736
                                      H SI  LK L  L+L GC     F  +   N+E 
Sbjct: 681  TKTPDFSAAPKLRRIILNGCTSLVKLHPSIGALKELIFLNLEGCSKLEKFPEVVQGNLED 740

Query: 737  LYELSLEGTSASNMPSSIGNLTELLSLNLSYCKELVSLPDSICNLKRLQRLDLNGCESLQ 796
            L  +SLEGT+   +PSSIG+L  L+ LNL  C++L SLP SIC L  LQ L L+GC  L+
Sbjct: 741  LSGISLEGTAIRELPSSIGSLNRLVLLNLRNCEKLASLPQSICELISLQTLTLSGCSKLK 800

Query: 797  HLPENIGKLESLWWLRADGTSIKNVPSSIICLKKIGTLSFSGCRGKGIKRSTSHWMSWLL 856
             LP+++G+L+ L  L  DGT IK V SSI  L  +  LS +GC+G G K  + + +S+  
Sbjct: 801  KLPDDLGRLQCLVELNVDGTGIKEVTSSINLLTNLEALSLAGCKGGGSK--SRNLISF-- 860

Query: 857  DRSNNHTTSFVGLQLPPLGGIQKLEALYLDNCNLLE--LPA-LDKLPSLESLDLRGNNFE 916
                  ++    LQLP L G+  L++L L +CNLLE  LP+ L  L SLE+L L  N+F 
Sbjct: 861  -----RSSPAAPLQLPFLSGLYSLKSLNLSDCNLLEGALPSDLSSLSSLENLYLDKNSFI 920

Query: 917  NLPQTIFSNLSSLKYLKLSYCRRLRSLPELPPNLRLLKADECTSLESFMSSVTLYKGIGR 976
             LP ++ S LS L+ L L +C+ LRSLPELP ++  L A  CTSLE+   S + Y    +
Sbjct: 921  TLPASL-SRLSRLRSLTLEHCKSLRSLPELPSSIEYLNAHSCTSLETLSCSSSTY--TSK 980

Query: 977  WNAKMYQFGNCLKL-KDQCNNILESMIKGIHLSEGIVQNLYDQGYCCRDGLNFSPWVAFP 1036
                 + F NC +L ++Q ++I+E++++G  L+  + + L       R  L        P
Sbjct: 981  LGDLRFNFTNCFRLGENQGSDIVETILEGTQLASSMAKLLEPDE---RGLLQHGYQALVP 1040

Query: 1037 GSEIPEWFSNQAVGKATTLHLPPRCLD-KSLGFALCFILSPNGTFQSFCNIGFELIFRDY 1065
            GS IP+WF++Q+VG    + LPP   + K +G A C + +  G    +    F L     
Sbjct: 1041 GSRIPKWFTHQSVGSKVIVELPPHWYNTKWMGLAACVVFNFKGAVDGYRGT-FPLACFLN 1100

BLAST of Lcy08g016420 vs. ExPASy TrEMBL
Match: A0A438IG52 (TMV resistance protein N OS=Vitis vinifera OX=29760 GN=N_195 PE=4 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 6.3e-197
Identity = 442/1171 (37.75%), Postives = 640/1171 (54.65%), Query Frame = 0

Query: 17   QYDVFVSFRGEDVRNNFVSHLQAALKRKGITTFSDEDQLKRGEEISPALVEAIQQSSISV 76
            +YD F+SFRGED R NF +HL AAL +KGI TF D + L RGE+IS  L++AI++S  S+
Sbjct: 21   KYDAFLSFRGEDTRKNFTTHLHAALCQKGINTFKD-NLLLRGEKISAGLLQAIEESRFSI 80

Query: 77   VVFSQNYASSKWCLEELSKIVERRDMDGQMVMPVFYHVHPSHVRNQTGSYAEAFRKHEET 136
            ++FS+NYASS WCL+EL+KI+E  +  G   +PVFY+V PSHVR Q G +A+AF +HE+ 
Sbjct: 81   IIFSENYASSSWCLDELTKILECVEEGGHTALPVFYNVDPSHVRKQKGCFADAFAEHEQV 140

Query: 137  LEDKKDRIQTWKDALKQIANLSGFHSANWRLESQMIDKITQEILKKLDRLTLDDVDGLVG 196
              +K +++  W+ AL ++A +SG+ S + R ES++I++I   IL +       +VD LVG
Sbjct: 141  YREKMEKVVKWRKALTEVATISGWDSRD-RDESEVIEEIVTRILNEPIDAFSSNVDALVG 200

Query: 197  IEHRVERIESSLCTDSNDVRILGIWGMGGIGKTTVARTLFHKIKNQFDGCCFLENIRENS 256
            ++ R+E + S LC  SNDVR +GIWGM GIGKTT+A  ++ +I  +FDGCCFL+++RE+S
Sbjct: 201  MDSRMEDLLSLLCIGSNDVRFVGIWGMAGIGKTTIAEAIYDRIYTKFDGCCFLKDVREDS 260

Query: 257  GKNGHGLPDLRKKLLSQVMEEAHLPMDISREFLKKRMCGKKVLIILDDIDDMEQLENLAG 316
             +  HGL  L++ LLS+V+   +  ++    F+K R+  KKVLI+LD++   ++LE L G
Sbjct: 261  QR--HGLTYLQETLLSRVLGGIN-NLNRGINFIKARLHSKKVLIVLDNVVHRQELEALVG 320

Query: 317  RHNWFGVGSRIVITTRNRQLLTEVDEVYDYAKEQLTYEEALLLFSHHAFNSSHPTEAYLE 376
             H+WFG GSRI+ITTR ++LL E +    Y  E+L Y+EAL LF  +AF   HPTE +++
Sbjct: 321  SHDWFGPGSRIIITTREKRLLIEQEMDAIYEVEKLEYDEALKLFCQYAFRYKHPTEDFMQ 380

Query: 377  LSRQAINYACGIPLALKILGSCLRTKDEKTWESALARLERFSNDKIVKRVLEISFEGLDW 436
            L   A++Y   +PLALK+LGSCL  K    W+S L +  +F N K V  VL+ SF+GLD 
Sbjct: 381  LCHHAVDYTGSLPLALKVLGSCLYRKSIHEWKSELDKFNQFPN-KEVLNVLKTSFDGLDD 440

Query: 437  SEKNILLDVACFFKGEDVKFATSIWNVCGFFPDYGISVLRDKCLISLLCNSQLWMHDLIQ 496
            +EKN+ LD+A F+KGED  F   + +   FFP   I  L DK LI+ + +++L+MHDL+Q
Sbjct: 441  NEKNMFLDIAFFYKGEDKDFVIEVLD--NFFPVSEIGNLVDKSLIT-ISDNKLYMHDLLQ 500

Query: 497  EMCKAIVRKEN-TDPGK----------------------------------------DAF 556
            EM   IVR+E+  DPGK                                        DAF
Sbjct: 501  EMGWEIVRQESIKDPGKRSRLRVHEDIHDVLTTNKGTEAVEGMVFDLSASKELNLSVDAF 560

Query: 557  VKMKNLRLLKFFSSKLDAS-----------------------------MRIRLPRGLTML 616
             KM  LRLL+F++ +   S                              ++ L R     
Sbjct: 561  AKMNKLRLLRFYNCQFYGSSEYLSEKELIASTHDARRWMGYDNSPYNDSKLHLSRDFKFP 620

Query: 617  PDSLRYLFWDGYPNKFLPSKFNAQNLLGLSLRQGCINQLWKEHSMFK------------- 676
             ++LR L W GYP K LPS F+ + L+ L++    + QLW+    F+             
Sbjct: 621  SNNLRSLHWHGYPLKSLPSNFHPEKLVELNMCYSLLKQLWEGKKAFEKLKFIKLSHSQHL 680

Query: 677  --------------------------HSSI-CLKHLKRLDLSGCPNFSNFSAICD-NMEH 736
                                      H SI  LK L  L+L GC     F  +   N+E 
Sbjct: 681  TKTPDFSAAPKLRRIILNGCTSLVKLHPSIGALKELIFLNLEGCSKLEKFPEVVQGNLED 740

Query: 737  LYELSLEGTSASNMPSSIGNLTELLSLNLSYCKELVSLPDSICNLKRLQRLDLNGCESLQ 796
            L  +SLEGT+   +PSSIG+L  L+ LNL  C++L SLP SIC L  LQ L L+GC  L+
Sbjct: 741  LSGISLEGTAIRELPSSIGSLNRLVLLNLRNCEKLASLPQSICELISLQTLTLSGCSKLK 800

Query: 797  HLPENIGKLESLWWLRADGTSIKNVPSSIICLKKIGTLSFSGCRGKGIKRSTSHWMSWLL 856
             LP+++G+L+ L  L  DGT IK V SSI  L  +  LS +GC+G G K  + + +S+  
Sbjct: 801  KLPDDLGRLQCLVELNVDGTGIKEVTSSINLLTNLEALSLAGCKGGGSK--SRNLISF-- 860

Query: 857  DRSNNHTTSFVGLQLPPLGGIQKLEALYLDNCNLLE--LPA-LDKLPSLESLDLRGNNFE 916
                  ++    LQLP L G+  L++L L +CNLLE  LP+ L  L SLE+L L  N+F 
Sbjct: 861  -----RSSPAAPLQLPFLSGLYSLKSLNLSDCNLLEGALPSDLSSLSSLENLYLDKNSFI 920

Query: 917  NLPQTIFSNLSSLKYLKLSYCRRLRSLPELPPNLRLLKADECTSLESFMSSVTLYKGIGR 976
             LP ++ S LS L+ L L +C+ LRSLPELP ++  L A  CTSLE+   S + Y    +
Sbjct: 921  TLPASL-SRLSRLRSLTLEHCKSLRSLPELPSSIEYLNAHSCTSLETLSCSSSTY--TSK 980

Query: 977  WNAKMYQFGNCLKL-KDQCNNILESMIKGIHLSEGIVQNLYDQGYCCRDGLNFSPWVAFP 1036
                 + F NC +L ++Q ++I+E++++G  L+  + + L       R  L        P
Sbjct: 981  LGDLRFNFTNCFRLGENQGSDIVETILEGTQLASSMAKLLEPDE---RGLLQHGYQALVP 1040

Query: 1037 GSEIPEWFSNQAVGKATTLHLPPRCLD-KSLGFALCFILSPNGTFQSFCNIGFELIFRDY 1065
            GS IP+WF++Q+VG    + LPP   + K +G A C + +  G    +    F L     
Sbjct: 1041 GSRIPKWFTHQSVGSKVIVELPPHWYNTKWMGLAACVVFNFKGAVDGYRGT-FPLACFLN 1100

BLAST of Lcy08g016420 vs. ExPASy TrEMBL
Match: M1NEA4 (TMV resistance protein N-like protein 7 OS=Vitis labrusca OX=103355 PE=2 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 1.5e-195
Identity = 466/1284 (36.29%), Postives = 662/1284 (51.56%), Query Frame = 0

Query: 1    MAESSSSQPISIWIQPQYDVFVSFRGEDVRNNFVSHLQAALKRKGITTFSDEDQLKRGEE 60
            MA SSSS   S   Q +YDVF+SFRGED R +F  HL  AL +KGI TF D DQL+RGE+
Sbjct: 1    MAPSSSSS--SSTHQWKYDVFLSFRGEDTRKSFTDHLHTALCQKGINTFMD-DQLRRGEQ 60

Query: 61   ISPALVEAIQQSSISVVVFSQNYASSKWCLEELSKIVERRDMDGQMVMPVFYHVHPSHVR 120
            +SPAL+ AI++S  S+++FS NYASS WCL+EL KI++   + G   +PVFY+V+PSHV+
Sbjct: 61   VSPALLNAIEESRFSIIIFSDNYASSSWCLDELVKILDCIKVMGHRALPVFYNVNPSHVK 120

Query: 121  NQTGSYAEAFRKHEETLEDKKDRIQTWKDALKQIANLSGFHSANWRLESQMIDKITQEIL 180
             QTGS+AEAF KHE+   +K +++  W++AL ++A +SG+ S + R ES++I++I ++I 
Sbjct: 121  KQTGSFAEAFAKHEQENREKMEKVVKWREALTEVATISGWDSRD-RHESKLIEEIVRDIW 180

Query: 181  KKLDRLTLDDVDGLVGIEHRVERIESSLCTDSNDVRILGIWGMGGIGKTTVARTLFHKIK 240
             KL   +   + GLVG+E R+E ++S LC  S DVR++GIWGM GIGKTT+A+ ++ +I 
Sbjct: 181  NKLVGTSPSYMKGLVGMESRLEAMDSLLCIGSLDVRMVGIWGMAGIGKTTIAKVIYERIY 240

Query: 241  NQFDGCCFLENIRENSGKNGHGLPDLRKKLLSQVMEEAHLPMDISRE---FLKKRMCGKK 300
             QF+GCCFL N+RE S K  HGLP L+ +LLSQ+++E +    +  +   F+K  +  +K
Sbjct: 241  TQFEGCCFLSNVREESYK--HGLPYLQMELLSQILKERNPNAGLFNKGINFMKDVLHSRK 300

Query: 301  VLIILDDIDDMEQLENLAGRHNWFGVGSRIVITTRNRQLLT--EVDEVYDYAKEQLTYEE 360
            VLIILDD+D  +QLE+LAG +NWFG GSRI+ITTR+R LLT  EVD +Y+   ++L  +E
Sbjct: 301  VLIILDDVDQRKQLEDLAGDNNWFGSGSRIIITTRDRHLLTCQEVDAIYEV--KELDNDE 360

Query: 361  ALLLFSHHAFNSSHPTEAYLELSRQAINYACGIPLALKILGSCLRTKDEKTWESALARLE 420
            AL LF  +AF   H TE + +L   A++Y  G+PLALK+LGS L TK    W+S L +L+
Sbjct: 361  ALKLFCLYAFRHKHGTEDFRQLCGHALDYTSGLPLALKVLGSSLYTKGIHEWKSELDKLK 420

Query: 421  RFSNDKIVKRVLEISFEGLDWSEKNILLDVACFFKGEDVKFATSIWNVCGFFPDYGISVL 480
            +F N K V+ VL+ SFEGLD +E+NI LD+A F+KG D  F   I + CGFF   GI  L
Sbjct: 421  QFPN-KEVQNVLKTSFEGLDDNEQNIFLDIAFFYKGHDKDFVGDILDSCGFFFGIGIRNL 480

Query: 481  RDKCLISLLCNSQLWMHDLIQEMCKAIVRKENTDPGK----------------------- 540
             DK LI+ +  ++L MHDL+QEM   IVR+++  PG+                       
Sbjct: 481  EDKSLIT-ISENKLCMHDLLQEMGWEIVRQKSEVPGERSRLRVHEDINHVLTTNTGTEAV 540

Query: 541  -----------------DAFVKMKNLRLLKFFSSKLDASM-------------------- 600
                             DAF KMK LRLLK  + ++D S+                    
Sbjct: 541  EGIFLDLSASKELNFSIDAFTKMKRLRLLKICNVQIDRSLGYLSKKELIAYTHDVWIERN 600

Query: 601  ------RIRLPRGLTMLPDSLRYLFWDGYPNKFLPSKFNAQNLLGLSLRQGCINQLWKEH 660
                  ++ L      L ++LR L+W GYP K  PS F+ + L+ L++    + Q W+  
Sbjct: 601  YLYTQNKLHLYEDSKFLSNNLRDLYWHGYPLKSFPSNFHPEKLVELNMCFSRLKQPWEGK 660

Query: 661  SMFK-------------------------------------------------------- 720
              F+                                                        
Sbjct: 661  KGFEKLKSIKLSHSQHLTKIPDFSGVPNLRRLILKGCTSLVEVHPSIGALKKLIFLNLEG 720

Query: 721  -------HSSICLKHLKRLDLSGCPNFSNFSAICDNMEHLYELSLEGTSAS--------- 780
                    SSI ++ L+ L LSGC     F  +  NMEHL  LSLEGT+           
Sbjct: 721  CKKLKSFSSSIHMESLQILTLSGCSKLKKFPEVQGNMEHLPNLSLEGTAIKGLPLSIENL 780

Query: 781  ------------------------------------------------------------ 840
                                                                        
Sbjct: 781  TGLALLNLKECKSLESLPRSIFKLKSLKTLILSNCTRLKKLPEIQENMESLMELFLDGSG 840

Query: 841  --NMPSSIGNLTELLSLNLSYCKELVSLPDSICNLKRLQRLDLNGCESLQHLPENIGKLE 900
               +PSSIG L  L+ LNL  CK+L SLP S C L  L+ L L GC  L+ LP+N+G L+
Sbjct: 841  IIELPSSIGCLNGLVFLNLKNCKKLASLPQSFCELTSLRTLTLCGCSELKDLPDNLGSLQ 900

Query: 901  SLWWLRADGTSIKNVPSSIICLKKIGTLSFSGCRGKGIKRSTSHWMSWLLDRSNNHTTSF 960
             L  L ADG+ ++ VP SI  L  +  LS +GC+G G  +S +   S+       H++  
Sbjct: 901  CLTELNADGSGVQEVPPSITLLTNLQILSLAGCKG-GESKSRNMIFSF-------HSSPT 960

Query: 961  VGLQLPPLGGIQKLEALYLDNCNLLE--LPA-LDKLPSLESLDLRGNNFENLPQTIFSNL 1020
              L+LP   G+  L  L L  CNL E  LP+ L  +PSLE LDL  N+F  +P ++ S L
Sbjct: 961  EELRLPSFSGLYSLRVLILQRCNLSEGALPSDLGSIPSLERLDLSRNSFITIPASL-SGL 1020

Query: 1021 SSLKYLKLSYCRRLRSLPELPPNLRLLKADECTSLESFMSSVTLYKGIGRWNAKMYQFGN 1065
            S L+ L L YC+ L+SLPELP ++  L A  CTSLE+F  S + Y    ++    + F N
Sbjct: 1021 SRLRSLTLEYCKSLQSLPELPSSVESLNAHSCTSLETFTCSSSAYTS-KKFGDLRFNFTN 1080

BLAST of Lcy08g016420 vs. NCBI nr
Match: XP_022152475.1 (TMV resistance protein N-like [Momordica charantia])

HSP 1 Score: 739.6 bits (1908), Expect = 3.9e-209
Identity = 421/793 (53.09%), Postives = 515/793 (64.94%), Query Frame = 0

Query: 168 ESQMIDKITQEILKKLDRLTLDDV-DGLVGIEHRVERIESSLCTDSNDVRILGIWGMGGI 227
           E+  I++I+ EILKKL+RLT  DV +GLVGIE R+ERIES +   S DV ILGIWGMGGI
Sbjct: 29  EATFIEEISGEILKKLNRLTPIDVHEGLVGIEARIERIESRVRVGSRDVCILGIWGMGGI 88

Query: 228 GKTTVARTLFHKIKNQFDGCCFLENIRENSGKNGHGLPDLRKKLLSQVMEEA--HLPMDI 287
           GKTT+AR LF +IK+QFDGCCFL+N+REN  K  HGL  L++KLL +V+EE    L  +I
Sbjct: 89  GKTTLARALFDRIKSQFDGCCFLKNVRENQIK--HGLSHLQEKLLCEVLEERRDQLIGNI 148

Query: 288 SREFLKKRMCGKKVLIILDDIDDMEQLENLAGRHNWFGVGSRIVITTRNRQLLTEVDEVY 347
           SR FLKKRM G+KVLI+LDD++DMEQLE+LAG  +WFG GSRI++TTR+RQ++   DEVY
Sbjct: 149 SRGFLKKRMRGRKVLIVLDDVNDMEQLEDLAGGRDWFGPGSRIIVTTRHRQVVNGADEVY 208

Query: 348 DYAKEQLTYEEALLLFSHHAF---NSSHPTEAYLELSRQAINYACGIPLALKILGSCLRT 407
           +   + L++EEA+ LFSHHAF      H  E +LELS +A+NYA GIPLALK+LGSCL  
Sbjct: 209 E--AQHLSHEEAMRLFSHHAFAFEGIDHSPEGFLELSTKAVNYAGGIPLALKVLGSCLCG 268

Query: 408 KDEKTWESALARLERFSNDKIVKRVLEISFEGLDWSEKNILLDVACFFKGEDVKFATSIW 467
           KD+  WES LA+LER  N+K V+ VL++SFEGLD +EK I LDVACFFKG+ V  A ++W
Sbjct: 269 KDKPVWESKLAKLERLPNEK-VQNVLKVSFEGLDRTEKGIFLDVACFFKGDHVGIAKAMW 328

Query: 468 NVCGFFPDYGISVLRDKCLISLLCNSQLWMHDLIQEMCKAIVRKEN-TDPGK-------- 527
           + CGFFP+ G+SVL DKCL+S+  NS   MHDLI+EM K IVR+E+  +PGK        
Sbjct: 329 DACGFFPEVGLSVLIDKCLLSITRNSVFQMHDLIEEMGKEIVRQEDMEEPGKRSRGTNAI 388

Query: 528 -----------------DAFVKMKNLRLLKFFSSKLDAS--MRIRLPRGLTMLPDSLRYL 587
                            DAF +M  LR L FFS  L  S   RIRLP GLTMLPDSL YL
Sbjct: 389 KGILLDLSKIKEVELGEDAFAEMTKLRFLNFFSKNLGESTKTRIRLPHGLTMLPDSLMYL 448

Query: 588 FWDGYPNKFLPSKFNAQNLLGLSLRQGCINQLWKEHSMF---KH---------------- 647
            WDGYP KF PS F+   L  L LR  CI QL K   +F   KH                
Sbjct: 449 SWDGYPYKFFPSNFDPDKLFWLRLRHSCIKQLSKGQKIFTNLKHINLSDSEYLSEVPDVS 508

Query: 648 --------------------------------------------SSICLKHLKRLDLSGC 707
                                                       SSICLK L+ LDLSGC
Sbjct: 509 EAPYLEDINFAGCIQLCTIHPSMATLSKLKTLNLRDCRKLRNLPSSICLKSLQELDLSGC 568

Query: 708 PNFSNFSAICDNMEHLYELSLEGTSASNMPSSIGNLTELLSLNLSYCKELVSLPDSICNL 767
            +  NF  I D MEHL+ LSL GT+ SN+PSSI N   L+ L+L+ CK LVSLPDSICNL
Sbjct: 569 SSLQNFPTISDYMEHLWSLSLSGTAISNLPSSIENFYGLVGLDLTNCKNLVSLPDSICNL 628

Query: 768 KRLQRLDLNGCESLQHLPENIGKLESLWWLRADGTSIKNVPSSIICLKKIGTLSFSGCRG 827
           K L  L L+ CE+LQHLPENIG LESL  +  D TSIK VPSSI+CLK I  LSF+GCRG
Sbjct: 629 KCLDSLILSSCENLQHLPENIGNLESLSTVYVDETSIKQVPSSIVCLKNIFELSFNGCRG 688

Query: 828 KGIKRSTSHWMSWLLDRSNNHTTSFVGLQLPPLGGIQKLEALYLDNCNLLELPA-LDKLP 863
                S   ++SW L RS + ++  +GLQLPPLGG ++L +L L NCNL+E P+ L  LP
Sbjct: 689 TETLSSLG-FLSWFLPRSRSSSSCNMGLQLPPLGGFKQLSSLSLSNCNLVEAPSGLHNLP 748

BLAST of Lcy08g016420 vs. NCBI nr
Match: XP_031249156.1 (disease resistance-like protein DSC1 isoform X2 [Pistacia vera])

HSP 1 Score: 722.6 bits (1864), Expect = 4.9e-204
Identity = 457/1149 (39.77%), Postives = 655/1149 (57.01%), Query Frame = 0

Query: 2    AESSSSQPISIWIQPQYDVFVSFRGEDVRNNFVSHLQAALKRKGITTFSDEDQLKRGEEI 61
            + SSSS P S+  Q +YDVF+SFRGED R +F SHL AAL +K I TF D+D L RG+EI
Sbjct: 3    SSSSSSSPSSMISQGKYDVFLSFRGEDTRASFTSHLYAALCQKQIQTFIDDDGLTRGDEI 62

Query: 62   SPALVEAIQQSSISVVVFSQNYASSKWCLEELSKIVERRDMDGQMVMPVFYHVHPSHVRN 121
            SPAL++AI++S IS+++FS++YASSKWCL+EL KI+E + + GQMV+PVFY+V+PS VR 
Sbjct: 63   SPALLKAIEESKISLIIFSKDYASSKWCLDELVKILECKKIKGQMVIPVFYNVNPSDVRK 122

Query: 122  QTGSYAEAFRKHEETLEDKKDRIQTWKDALKQIANLSGFHSANWRLESQMIDKITQEILK 181
            QTGS+ +AF KHEE  + K +++Q ++ AL + +NLSG+ S   R +S+++D+I +++LK
Sbjct: 123  QTGSFKDAFVKHEEYFKGKIEKVQRFRTALTEASNLSGWDSLVTRPDSKLVDEIVKDVLK 182

Query: 182  KLDRLT-LDDVDGLVGIEHRVERIESSLCTDSNDVRILGIWGMGGIGKTTVARTLFHKIK 241
            KL+ ++   D +GLVG+E RVE+++S LC+  +D R++GIWGMGGIGKTT+A  +F ++ 
Sbjct: 183  KLNDISPSSDCEGLVGLESRVEQVKSLLCSGFSDFRVVGIWGMGGIGKTTIAEAIFKQVS 242

Query: 242  NQFDGCCFLENIRENSGKNGHGLPDLRKKLLSQVMEEAHLPM---DISREFLKKRMCGKK 301
             QFDGCCF+EN+RE S K G GL  LR++++S+V++E +L +   ++  +F+K R+  KK
Sbjct: 243  GQFDGCCFIENVREESKKCG-GLARLREQVISRVLDERNLSIGTPNMIPQFIKHRLRCKK 302

Query: 302  VLIILDDIDDMEQLENLAGRHNWFGVGSRIVITTRNRQLLTE--VDEVYDYAKEQLTYEE 361
            V IILDD++D EQLE L G  + FG GSR++ITTR++Q+L    VD    Y  E+  + E
Sbjct: 303  VFIILDDVNDSEQLEFLTGGLDRFGPGSRVIITTRDKQVLGNFGVDNDNIYEVERFKHHE 362

Query: 362  ALLLFSHHAFNSSHPTEAYLELSRQAINYACGIPLALKILGSCLRTKDEKTWESALARLE 421
            AL LF + AF  +HP   +LELS + ++YA G PLALK+L S L  K ++ WESAL +L+
Sbjct: 363  ALQLFCNFAFQQNHPPNDFLELSSKVVDYAKGNPLALKVLASSLHKKSKRDWESALNKLK 422

Query: 422  RFSNDKIVKRVLEISFEGLDWSEKNILLDVACFFKGEDVKFATSIWNVCGFFPDYGISVL 481
            + SN KI   +L IS++GLD  E++I LD+ACFF+GED    T I + C F   YG+SVL
Sbjct: 423  KISNPKI-HNILRISYDGLDREEQDIFLDIACFFEGEDSNHVTRILDGCYFSAHYGLSVL 482

Query: 482  RDKCLISLLCNSQLWMHDLIQEMCKAIVRKEN-TDPGK---------------------- 541
             DK LI++   ++L MHDL+QEM   IVR+ +  +PGK                      
Sbjct: 483  IDKSLITISSKNKLQMHDLLQEMGWEIVRQVSFKEPGKHSRLWNPEDVCNVLKKNTGTDA 542

Query: 542  ------------------DAFVKMKNLRLLKF-----FSSKLDASMRIRLPRGLTMLPDS 601
                              +AF  M+NLRLLKF     FS     + ++  P+GL  L D 
Sbjct: 543  IEGLFLDMSKIREMHLSSEAFTNMRNLRLLKFYVSDQFSGGFVNNPKLCFPQGLEYLSDE 602

Query: 602  LRYLFWDGYPNKFLPSKFNAQNLLGLSLRQGCINQLW--KEHSM---------------F 661
            LRYL+W G+P K LP +F+ + L+ L+L    I QLW   +H +                
Sbjct: 603  LRYLYWVGFPLKTLPPRFSPEKLIELNLPYSNIEQLWIGTKHLIEIPDVSEAPNIEIIEL 662

Query: 662  KH--------SSI-CLKHLKRLDLSGCPNFSNF-------SAICDNME------------ 721
            +H        SSI  L HL+ L LSGC +  NF       S +  N+             
Sbjct: 663  QHCTNLVKVPSSIQYLNHLRILRLSGCKSLRNFPSNIHFKSLVTLNLSSCIYLTEFPRIS 722

Query: 722  -HLYELSLEGTSASNMPSSIGNLTELLSLNLSYCKELVSLPDSICNLKRLQRLDLNGCES 781
             ++ +++L GT+   +PSSI  LTEL SL ++ C  L S+  SIC LK LQRL+L+ C  
Sbjct: 723  GNIRDINLSGTAIEEVPSSIMCLTELCSLVMTRCSRLKSISTSICKLKSLQRLELDYCSK 782

Query: 782  LQHLPENIGKLESLWWLRADG-TSIKNVPSSIICL-----------KKIGTLSFSGCRGK 841
            L+  PE + K+ESL  L  +G T I+ +PSSI  L           K + T+  S C   
Sbjct: 783  LESFPEILEKMESLERLLLNGATEIRELPSSIEHLNGLRHLRLMDCKNLETIPSSICNLT 842

Query: 842  GIKRSTSHWMSWLLDRSNNHTTSF--------VGLQLPPLGGIQKLEALYLDNCNLLELP 901
             +        S L+  S N TT +         G  LPPL  +  L  L+L  CNL E+P
Sbjct: 843  SLIEMDLSGCSKLVKLSENSTTQWKGNIYSQCPGFVLPPLSALCSLTELFLSFCNLTEIP 902

Query: 902  A-LDKLPSLESLDLRGNNFENLPQTIFSNLSSLKYLKLSYCRRLRSLPELPPNLRLLKAD 961
              L  + SL++L+L GNNF+ LP  I   LS LK L L  C  L+SLPELPP LR+L A 
Sbjct: 903  EDLCCISSLKTLNLNGNNFKTLPSRI-KQLSKLKRLYLRDCNMLQSLPELPPCLRVLIAM 962

Query: 962  ECTSLESFMSSVTLYKGIG--------------RWNAKMYQFGNCLKLKDQCNNILESMI 1011
             C  L+S     +  + +                W    + F NC KL     NILE  +
Sbjct: 963  NCKRLQSLPKLPSSLEELDASELEVVSERYVPFSWITMRFLFTNCQKLNQNAYNILE--L 1022

BLAST of Lcy08g016420 vs. NCBI nr
Match: XP_031249155.1 (disease resistance-like protein DSC1 isoform X1 [Pistacia vera])

HSP 1 Score: 719.2 bits (1855), Expect = 5.4e-203
Identity = 456/1163 (39.21%), Postives = 652/1163 (56.06%), Query Frame = 0

Query: 2    AESSSSQPISIWIQPQYDVFVSFRGEDVRNNFVSHLQAALKRKGITTFSDEDQLKRGEEI 61
            + SSSS P S+  Q +YDVF+SFRGED R +F SHL AAL +K I TF D+D L RG+EI
Sbjct: 3    SSSSSSSPSSMISQGKYDVFLSFRGEDTRASFTSHLYAALCQKQIQTFIDDDGLTRGDEI 62

Query: 62   SPALVEAIQQSSISVVVFSQNYASSKWCLEELSKIVERRDMDGQMVMPVFYHVHPSHVRN 121
            SPAL++AI++S IS+++FS++YASSKWCL+EL KI+E + + GQMV+PVFY+V+PS VR 
Sbjct: 63   SPALLKAIEESKISLIIFSKDYASSKWCLDELVKILECKKIKGQMVIPVFYNVNPSDVRK 122

Query: 122  QTGSYAEAFRKHEETLEDKKDRIQTWKDALKQIANLSGFHSANWRLESQMIDKITQEILK 181
            QTGS+ +AF KHEE  + K +++Q ++ AL + +NLSG+ S   R +S+++D+I +++LK
Sbjct: 123  QTGSFKDAFVKHEEYFKGKIEKVQRFRTALTEASNLSGWDSLVTRPDSKLVDEIVKDVLK 182

Query: 182  KLDRLT-LDDVDGLVGIEHRVERIESSLCTDSNDVRILGIWGMGGIGKTTVARTLFHKIK 241
            KL+ ++   D +GLVG+E RVE+++S LC+  +D R++GIWGMGGIGKTT+A  +F ++ 
Sbjct: 183  KLNDISPSSDCEGLVGLESRVEQVKSLLCSGFSDFRVVGIWGMGGIGKTTIAEAIFKQVS 242

Query: 242  NQFDGCCFLENIRENSGKNGHGLPDLRKKLLSQVMEEAHLPM---DISREFLKKRMCGKK 301
             QFDGCCF+EN+RE S K G GL  LR++++S+V++E +L +   ++  +F+K R+  KK
Sbjct: 243  GQFDGCCFIENVREESKKCG-GLARLREQVISRVLDERNLSIGTPNMIPQFIKHRLRCKK 302

Query: 302  VLIILDDIDDMEQLENLAGRHNWFGVGSRIVITTRNRQLLTE--VDEVYDYAKEQLTYEE 361
            V IILDD++D EQLE L G  + FG GSR++ITTR++Q+L    VD    Y  E+  + E
Sbjct: 303  VFIILDDVNDSEQLEFLTGGLDRFGPGSRVIITTRDKQVLGNFGVDNDNIYEVERFKHHE 362

Query: 362  ALLLFSHHAFNSSHPTEAYLELSRQAINYACGIPLALKILGSCLRTKDEKTWESALARLE 421
            AL LF + AF  +HP   +LELS + ++YA G PLALK+L S L  K ++ WESAL +L+
Sbjct: 363  ALQLFCNFAFQQNHPPNDFLELSSKVVDYAKGNPLALKVLASSLHKKSKRDWESALNKLK 422

Query: 422  RFSNDKIVKRVLEISFEGLDWSEKNILLDVACFFKGEDVKFATSIWNVCGFFPDYGISVL 481
            + SN KI   +L IS++GLD  E++I LD+ACFF+GED    T I + C F   YG+SVL
Sbjct: 423  KISNPKI-HNILRISYDGLDREEQDIFLDIACFFEGEDSNHVTRILDGCYFSAHYGLSVL 482

Query: 482  RDKCLISLLCNSQLWMHDLIQEMCKAIVRKEN-TDPGK---------------------- 541
             DK LI++   ++L MHDL+QEM   IVR+ +  +PGK                      
Sbjct: 483  IDKSLITISSKNKLQMHDLLQEMGWEIVRQVSFKEPGKHSRLWNPEDVCNVLKKNTGTDA 542

Query: 542  ------------------DAFVKMKNLRLLKF-----FSSKLDASMRIRLPRGLTMLPDS 601
                              +AF  M+NLRLLKF     FS     + ++  P+GL  L D 
Sbjct: 543  IEGLFLDMSKIREMHLSSEAFTNMRNLRLLKFYVSDQFSGGFVNNPKLCFPQGLEYLSDE 602

Query: 602  LRYLFWDGYPNKFLPSKFNAQNLLGLSLRQGCINQLW-KEHSMFKHSSI----------- 661
            LRYL+W G+P K LP +F+ + L+ L+L    I QLW    S F+  SI           
Sbjct: 603  LRYLYWVGFPLKTLPPRFSPEKLIELNLPYSNIEQLWIGTKSAFELKSIDLYHSQHLIEI 662

Query: 662  ----------------------------CLKHLKRLDLSGCPNFSNF-------SAICDN 721
                                         L HL+ L LSGC +  NF       S +  N
Sbjct: 663  PDVSEAPNIEIIELQHCTNLVKVPSSIQYLNHLRILRLSGCKSLRNFPSNIHFKSLVTLN 722

Query: 722  ME-------------HLYELSLEGTSASNMPSSIGNLTELLSLNLSYCKELVSLPDSICN 781
            +              ++ +++L GT+   +PSSI  LTEL SL ++ C  L S+  SIC 
Sbjct: 723  LSSCIYLTEFPRISGNIRDINLSGTAIEEVPSSIMCLTELCSLVMTRCSRLKSISTSICK 782

Query: 782  LKRLQRLDLNGCESLQHLPENIGKLESLWWLRADG-TSIKNVPSSIICL----------- 841
            LK LQRL+L+ C  L+  PE + K+ESL  L  +G T I+ +PSSI  L           
Sbjct: 783  LKSLQRLELDYCSKLESFPEILEKMESLERLLLNGATEIRELPSSIEHLNGLRHLRLMDC 842

Query: 842  KKIGTLSFSGCRGKGIKRSTSHWMSWLLDRSNNHTTSF--------VGLQLPPLGGIQKL 901
            K + T+  S C    +        S L+  S N TT +         G  LPPL  +  L
Sbjct: 843  KNLETIPSSICNLTSLIEMDLSGCSKLVKLSENSTTQWKGNIYSQCPGFVLPPLSALCSL 902

Query: 902  EALYLDNCNLLELPA-LDKLPSLESLDLRGNNFENLPQTIFSNLSSLKYLKLSYCRRLRS 961
              L+L  CNL E+P  L  + SL++L+L GNNF+ LP  I   LS LK L L  C  L+S
Sbjct: 903  TELFLSFCNLTEIPEDLCCISSLKTLNLNGNNFKTLPSRI-KQLSKLKRLYLRDCNMLQS 962

Query: 962  LPELPPNLRLLKADECTSLESFMSSVTLYKGIG--------------RWNAKMYQFGNCL 1011
            LPELPP LR+L A  C  L+S     +  + +                W    + F NC 
Sbjct: 963  LPELPPCLRVLIAMNCKRLQSLPKLPSSLEELDASELEVVSERYVPFSWITMRFLFTNCQ 1022

BLAST of Lcy08g016420 vs. NCBI nr
Match: XP_010665420.1 (PREDICTED: TMV resistance protein N-like [Vitis vinifera])

HSP 1 Score: 718.8 bits (1854), Expect = 7.1e-203
Identity = 454/1138 (39.89%), Postives = 637/1138 (55.98%), Query Frame = 0

Query: 1    MAESSSSQPISIWIQP---QYDVFVSFRGEDVRNNFVSHLQAALKRKGITTFSDEDQLKR 60
            MA SS+   +     P   +YDVF+SFRGED R +F +HL +AL +KGI TF D   L R
Sbjct: 1    MASSSTILSVPSSSSPHRWKYDVFLSFRGEDTRQSFTAHLHSALSQKGINTFKD-SLLPR 60

Query: 61   GEEISPALVEAIQQSSISVVVFSQNYASSKWCLEELSKIVERRDMDGQMVMPVFYHVHPS 120
            GE+ISPAL++AI++S  S++V S+NYASS WCLEEL+KI+E  +  G   +PVF++V PS
Sbjct: 61   GEKISPALLQAIEESRFSIIVLSENYASSSWCLEELTKILECVEEGGHTALPVFHNVDPS 120

Query: 121  HVRNQTGSYAEAFRKHEETLEDKKDRIQTWKDALKQIANLSGFHSANWRLESQMIDKITQ 180
            +VR Q GS+A+AF KHE+  +DK +++  W+DAL + A ++G+ + N R ES++I++I  
Sbjct: 121  NVRKQEGSFAKAFAKHEQVYKDKMEQVVKWRDALTEAATIAGWDTRN-RDESEVIEQIVT 180

Query: 181  EILKKLDRLTLDDVDGLVGIEHRVERIESSLCTDSNDVRILGIWGMGGIGKTTVARTLFH 240
             IL +       ++D LVG++ R+E + S LC  S+DVR +GIWGM GIGKTT+A  ++ 
Sbjct: 181  RILNEPIDAFSSNMDALVGMDSRMEDLLSRLCIGSDDVRFVGIWGMAGIGKTTIAEAIYD 240

Query: 241  KIKNQFDGCCFLENIRENSGKNGHGLPDLRKKLLSQVMEEAHLPMDISREFLKKRMCGKK 300
            +I  +FDGCCFL+N+RE+S +  HGL  L++ LLSQV+   +  ++    F+K R+  K+
Sbjct: 241  RIYTKFDGCCFLKNVREDSQR--HGLTYLQETLLSQVLGGIN-NLNRGINFIKARLRPKR 300

Query: 301  VLIILDDIDDMEQLENLAGRHNWFGVGSRIVITTRNRQLL--TEVDEVYDYAKEQLTYEE 360
            VLI+LDD+   +QLE LAG H+WFG GSRI+ITTR ++LL   EVDE+Y    E+L Y+E
Sbjct: 301  VLIVLDDVVHRQQLEALAGNHDWFGSGSRIIITTREKRLLIEQEVDEIYKV--EKLEYDE 360

Query: 361  ALLLFSHHAFNSSHPTEAYLELSRQAINYACGIPLALKILGSCLRTKDEKTWESALARLE 420
            AL LF  +AF   HPTE +++L   A++Y  G+PLALK+LGSCL  K    W+S L +L 
Sbjct: 361  ALKLFCQYAFRYKHPTEDFMQLCHHAVDYTGGLPLALKVLGSCLYRKSIHEWKSELDKLN 420

Query: 421  RFSNDKIVKRVLEISFEGLDWSEKNILLDVACFFKGEDVKFATSIWNVCGFFPDYGISVL 480
            +F N K V  VL+ SF+GLD +EKN+ LD+A F+KGED  F   + +   FFP   I  L
Sbjct: 421  QFPN-KEVLNVLKTSFDGLDDNEKNMFLDIAFFYKGEDKDFVIEVLD--NFFPVSEIGNL 480

Query: 481  RDKCLISLLCNSQLWMHDLIQEMCKAIVRKEN-TDPGK---------------------- 540
             DK LI+ + +++L+MHDL+QEM   IVR+E+  DPGK                      
Sbjct: 481  VDKSLIT-ISDNKLYMHDLLQEMGWEIVRQESIKDPGKRSRLRVHEDIHDVLTTNKGTEA 540

Query: 541  ------------------DAFVKMKNLRLLKFFS------------SKLDASMR------ 600
                              DAF KM  LRLL+F++             +L AS R      
Sbjct: 541  VEGMVFDLSASKELNLSVDAFAKMNKLRLLRFYNCQFYGSSEYLSEEELIASTRDAWRWM 600

Query: 601  -----------IRLPRGLTMLPDSLRYLFWDGYPNKFLPSKFNAQNLLGLSLRQGCINQL 660
                       + L R      ++LR L W GYP K LPS F+ + L+ L++    + QL
Sbjct: 601  GYDNSPYNDSKLHLSRDFKFPSNNLRSLHWHGYPLKSLPSIFHPKKLVELNMCYSLLKQL 660

Query: 661  WKEHSMFK---------------------------------------HSSI-CLKHLKRL 720
            W+    F+                                       H SI  LK L  L
Sbjct: 661  WEGKKAFEKLKFIKLSHSQHLTKTPDFSAAPKLRRIILNGCTSLVKLHPSIGALKELIFL 720

Query: 721  DLSGCPNFSNFSAICD-NMEHLYELSLEGTSASNMPSSIGNLTELLSLNLSYCKELVSLP 780
            +L GC     F  +   N+E L  +SLEGT+   +PSSIG L  L+ LNL  CK+L SLP
Sbjct: 721  NLEGCSKLEKFPEVVQGNLEDLSGISLEGTAIRELPSSIGGLNRLVLLNLRNCKKLASLP 780

Query: 781  DSICNLKRLQRLDLNGCESLQHLPENIGKLESLWWLRADGTSIKNVPSSIICLKKIGTLS 840
             SIC L  LQ L L+GC  L+ LP+++G+L+ L  L  DGT IK VPSSI  L  +  LS
Sbjct: 781  QSICELISLQTLTLSGCSKLKKLPDDLGRLQCLVELHVDGTGIKEVPSSINLLTNLQELS 840

Query: 841  FSGCRGKGIKRSTSHWMSWLLDRSNNHTTSFVGLQLPPLGGIQKLEALYLDNCNLLE--L 900
             +GC+G   K       SW L  S     +   L+LP L G+  L+ L L +CNLLE  L
Sbjct: 841  LAGCKGWESK-------SWNLAFSFGSWPTLEPLRLPRLSGLYSLKILNLSDCNLLEGAL 900

Query: 901  PA-LDKLPSLESLDLRGNNFENLPQTIFSNLSSLKYLKLSYCRRLRSLPELPPNLRLLKA 960
            P  L  L SLE LDL  N+F  +P  + S LS L  L L YC+ L+SLPELP ++R L A
Sbjct: 901  PIDLSSLSSLEMLDLSRNSFITIPANL-SGLSRLHVLMLPYCKSLQSLPELPSSIRYLNA 960

Query: 961  DECTSLESFMSSVTLYKGIGRWNAKMYQFGNCLKL-KDQCNNILESMIKGIHLSEGIVQN 1018
            + CTSLE+F  S +      R+     +F NC +L +++ N+ ++ ++ GI L   I + 
Sbjct: 961  EACTSLETFSCSPSACTS-KRYGGLRLEFSNCFRLMENEHNDSVKHILLGIQLLASIPKF 1020

BLAST of Lcy08g016420 vs. NCBI nr
Match: XP_024042935.1 (disease resistance-like protein DSC1 [Citrus clementina])

HSP 1 Score: 716.5 bits (1848), Expect = 3.5e-202
Identity = 457/1127 (40.55%), Postives = 632/1127 (56.08%), Query Frame = 0

Query: 2    AESSSSQPISIWIQPQYDVFVSFRGEDVRNNFVSHLQAALKRKGITTFSDEDQLKRGEEI 61
            + SS+S   S   Q +YDVF+SFRGED R+NF SHL AAL R  I TF D  QL+RG+E+
Sbjct: 4    SSSSASASASTTRQWKYDVFLSFRGEDTRSNFTSHLYAALCRAKIETFIDY-QLRRGDEV 63

Query: 62   SPALVEAIQQSSISVVVFSQNYASSKWCLEELSKIVERRDMD--GQMVMPVFYHVHPSHV 121
            SPAL++AI+ S+IS+V+ S+ YASS WCL+EL KI+E +D    GQ+V+PVFYHV+PS V
Sbjct: 64   SPALLKAIEDSNISIVILSKGYASSSWCLDELLKILECKDTTDMGQIVLPVFYHVNPSDV 123

Query: 122  RNQTGSYAEAFRKHEETLEDKKDRIQTWKDALKQIANLSGFH-SANWRLESQMIDKITQE 181
            R QTGS+ EA  KHE+     K ++  W+ AL Q+ANLSG+H       E+++++KI ++
Sbjct: 124  RKQTGSFGEALAKHEKYSSKTKPKVLKWRAALTQVANLSGWHLDKQLGSEAELVEKIVKD 183

Query: 182  ILKKLDRLTLDDVDGLVGIEHRVERIESSLCTDSNDVRILGIWGMGGIGKTTVARTLFHK 241
            +LKKL+  +   +DGL+GIE RVE++ES LC    DV I+GIWGMGGIGKTT+AR +F +
Sbjct: 184  VLKKLNHTSSGALDGLIGIESRVEKVESLLCIGLVDVHIVGIWGMGGIGKTTIARAIFDR 243

Query: 242  IKNQFDGCCFLENIRENSGKNGHGLPDLRKKLLSQVMEEAHLPMDIS---REFLKKRMCG 301
            I NQF+GCCFLEN+RE S K   G+  L+++L S+++E+  L +  S     F+K R+  
Sbjct: 244  IANQFEGCCFLENVREESAK--RGVHRLQEELFSRLLEDGDLSLGASGLGHTFMKTRLRR 303

Query: 302  KKVLIILDDIDDMEQLENLAGRHNWFGVGSRIVITTRNRQLL-TEVDEVYDYAKEQLTYE 361
            K VLI+LDD+++ +QL+NLAG H WFG+GSRI+IT+R++Q+L T VDE+Y+   E+L   
Sbjct: 304  KTVLIVLDDVENSQQLKNLAGDHGWFGLGSRIIITSRDKQVLKTGVDEMYEV--EELNCR 363

Query: 362  EALLLFSHHAFNSSHPTEAYLELSRQAINYACGIPLALKILGSCLRTKDEKTWESALARL 421
            EAL LFS +AF  +HPTE Y+ LS Q ++YA GIPLALK+LG  L  + ++ WESAL +L
Sbjct: 364  EALQLFSLNAFKLNHPTEDYMGLSNQVVHYAKGIPLALKVLGCFLFGRSKRDWESALNKL 423

Query: 422  ERFSNDKIVKRVLEISFEGLDWSEKNILLDVACFFKGEDVKFATSIWNVCGFFPDYGISV 481
             +  N  I + VL I+++ LD  EK I LD+ACFFKG++    T+I + CGF  + GISV
Sbjct: 424  RKNPNMDI-QNVLRITYDALDDEEKAIFLDIACFFKGDNRDHVTTILDGCGFSTEIGISV 483

Query: 482  LRDKCLISLLCNSQLWMHDLIQEMCKAIVRKEN-TDPGK--------------------- 541
            L DKCLI+ + + +L MHDL+QEM   IVR+E+  DPGK                     
Sbjct: 484  LIDKCLIT-VTDDRLLMHDLLQEMGWGIVRQESIKDPGKRSRLWDPQDVCNLFKKNSGSE 543

Query: 542  -------------------DAFVKMKNLRLLKFFSSKLDASM----RIRLPRGLTMLPDS 601
                               DAF+ M  LRLLKFFSS          ++ L +GL +L + 
Sbjct: 544  VVESISLDLSKTSELHLRSDAFLGMHQLRLLKFFSSSYREGYVEEDKVHLCQGLEILSNE 603

Query: 602  LRYLFWDGYPNKFLPSKFNAQNLLGLSLRQGCINQLWKE--------------------- 661
            LRYL W  YP K LPS FN +NL+ L +    +  LW+E                     
Sbjct: 604  LRYLHWHRYPLKSLPSNFNPENLVELDMHHSNLEHLWEEMQHALNLRRIDLSYSLHLNET 663

Query: 662  -----------------HSMFKH------------------------------------- 721
                             +S+ K                                      
Sbjct: 664  PDLSSARNLEIMVLDGCYSLIKFPKTPWSITELDLGETAIEEVPPAIESLCKLVVLRLDN 723

Query: 722  --------SSIC-LKHLKRLDLSGCPNFSNFSAICDNMEHLYELSLEGTSASNMPSSIGN 781
                    SSIC L  L  L+L GC N + F  I  +M++   LSL  T+   +PSS+  
Sbjct: 724  CRRLKNLPSSICNLTSLTELELHGCSNITKFPDISGDMKY---LSLSETAIEELPSSVEC 783

Query: 782  LTELLSLNLSYCKELVSLPDSICNLKRLQRLDLNGCESLQHLPENIGKLESLWWLRADGT 841
            LTEL  L L  CK L  +  SIC LK L+ L L GC  L+ LPE +  +E L  L   GT
Sbjct: 784  LTELTVLRLQKCKRLKRVSSSICKLKSLEILYLFGCSKLEGLPEILESMERLETLYLAGT 843

Query: 842  SIKNVPSSIICLKKIGTLSFSGCRGKGIKRSTSHWMSWLLDRSNNHTTSFVG-------L 901
             IK +PSSI  L ++  LS   C  K I    + + + L   S+ + + + G       L
Sbjct: 844  PIKELPSSIDHLPQLSLLSLENC--KNILSFPNSFFN-LTSLSSLNLSGWSGVDKLLENL 903

Query: 902  QLPPLGGIQKLEALYLDNCNLLELP-ALDKLPSLESLDLRGNNFENLPQTIFSNLSSLKY 961
             L  L G+  L  L+L++CNLLELP AL  L SLE LDL GN FE+L    F   S L +
Sbjct: 904  PLALLSGLCSLTELHLNDCNLLELPSALTCLSSLEILDLSGNIFESLNLKPF---SCLTH 963

Query: 962  LKLSYCRRLRSLPELPPNLRL--LKADECTSLESFMSSVTLYKGIGRWNAKMYQFGNCLK 975
            L +SYC+RL+SL E P  LRL  L+A EC  LE+  +S  +   +   + + + F NC K
Sbjct: 964  LNVSYCKRLQSLQEFPSPLRLVNLQAHECIYLETVPASADVEFTVSWSSQQYFTFFNCWK 1023

BLAST of Lcy08g016420 vs. TAIR 10
Match: AT4G12010.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 546.2 bits (1406), Expect = 5.9e-155
Identity = 402/1193 (33.70%), Postives = 602/1193 (50.46%), Query Frame = 0

Query: 6    SSQPISIWIQPQYDVFVSFRGEDVRNNFVSHLQAALKRKGITTFSDEDQLKRGEEISPAL 65
            SS P S     ++DVF+SFRG D RNNF  HLQ AL+ +GI +F D D+L+RG+ ++ AL
Sbjct: 3    SSSPSS----AEFDVFLSFRGFDTRNNFTGHLQKALRLRGIDSFID-DRLRRGDNLT-AL 62

Query: 66   VEAIQQSSISVVVFSQNYASSKWCLEELSKIVERRDMDGQMVMPVFYHVHPSHVRNQTGS 125
             + I++S I+++VFS NYA+S WCL EL KI+E R+ + Q+V+P+FY V  S V  Q  S
Sbjct: 63   FDRIEKSKIAIIVFSTNYANSAWCLRELVKILECRNSNQQLVVPIFYKVDKSDVEKQRNS 122

Query: 126  YAEAFRKHEETLED-KKDRIQTWKDALKQIANLSGFHSANWRL-ESQMIDKITQEILKKL 185
            +A  F+  E T      + I +WK AL   +N+ G+        E++++D+I  +  KKL
Sbjct: 123  FAVPFKLPELTFPGVTPEEISSWKAALASASNILGYVVKEISTSEAKLVDEIAVDTFKKL 182

Query: 186  DRLTLDDVDGLVGIEHRVERIESSLC-TDSNDVRILGIWGMGGIGKTTVARTLFHKIKNQ 245
            + L     +GLVGIE R++ +E  L   D + V I+GI GM GIGKTT+A  L+ +++ Q
Sbjct: 183  NDLAPSGNEGLVGIESRLKNLEKLLSWEDLDTVHIIGIVGMVGIGKTTLADCLYGRMRGQ 242

Query: 246  FDGCCFLENIRENSGKNGHGLPDLRKKLLSQVMEEAHLPMDI---SREFLKKRMCGKKVL 305
            FDG CFL NIRENSG++  GL  L +KL S V+ +  L +     + E  ++R+  K++L
Sbjct: 243  FDGSCFLTNIRENSGRS--GLESLLQKLFSTVLNDRDLEIGAPGNAHERFERRLKSKRLL 302

Query: 306  IILDDIDDMEQLENLAGRHNWFGVGSRIVITTRNRQLLTEVDEVYDYAKEQLTYEEALLL 365
            I+LDD++D +Q+  L G   W+  GSRI+ITTR+ +L+ E  +   Y   +L   EAL L
Sbjct: 303  IVLDDVNDEKQIRYLMGHCKWYQGGSRIIITTRDSKLI-ETIKGRKYVLPKLNDREALKL 362

Query: 366  FSHHAFNSSHPTEAYLELSRQAINYACGIPLALKILGSCLRTKDEKTWESALARLERFSN 425
            FS +AF++S P + +  L+   ++YA G PLALK+LGS L  +D+  WE+ L RL+  S+
Sbjct: 363  FSLNAFSNSFPLKEFEGLTNMVLDYAKGHPLALKVLGSDLCERDDLYWEAKLDRLKSRSH 422

Query: 426  DKIVKRVLEISFEGLDWSEKNILLDVACFFKGEDVKFATSIWNVCGFFPDYGISVLRDKC 485
              I + VLE S+E L   +KN+ LD+ACFF+ E+V + TS+ N  G      +  L DKC
Sbjct: 423  GDIYE-VLETSYEELTTEQKNVFLDIACFFRSENVDYVTSLLNSHGVDVSGVVKDLVDKC 482

Query: 486  LISLLCNSQLWMHDLIQEMCKAIVRKENTDPGKD-------------------------- 545
            LI+ L ++++ MHD++Q M K I  K  T   +D                          
Sbjct: 483  LIT-LSDNRIEMHDMLQTMAKEISLKVETIGIRDCRWLSRHGNQCQWHIRLWDSEDICDL 542

Query: 546  ----------------------------AFVKMKNLRLLKFFSSK----LDASMRIRLPR 605
                                        AF  M NL+ LK + S      +A  ++ L R
Sbjct: 543  LTEGLGTDKIRGIFLDTSKLRAMRLSAKAFQGMYNLKYLKIYDSHCSRGCEAEFKLHLRR 602

Query: 606  GLTMLPDSLRYLFWDGYPNKFLPSKFNAQNLLGLSLRQGCINQLWKEHSMFKHSSICLKH 665
            GL+ LP+ L YL W GYP + +P  F+ +NL+ L L    + ++W +          +  
Sbjct: 603  GLSFLPNELTYLHWHGYPLQSIPLDFDPKNLVDLKLPHSQLEEIWDDEK-------DVGM 662

Query: 666  LKRLDLSGCPNFSNFSAICDNMEHLYELSLEG-TSASNMPSSIGNLTELLSLNLSYCKEL 725
            LK +DLS   N      +  N  +L  L+LEG TS   +PS+I  L +L+ LNL  C  L
Sbjct: 663  LKWVDLSHSINLRQCLGLA-NAHNLERLNLEGCTSLKKLPSTINCLEKLIYLNLRDCTSL 722

Query: 726  VSLPDSICNLKRLQRLDLNGCESLQHLPENIGKLESLWWLRADGTSIKNVPSSIICLKKI 785
             SLP  I   + LQ L L+GC SL+  P     +E    L  DGT IK++P SI   +++
Sbjct: 723  RSLPKGI-KTQSLQTLILSGCSSLKKFPLISENVEV---LLLDGTVIKSLPESIQTFRRL 782

Query: 786  GTLSFSGCRGKGIKRSTSHW---------------------------------------- 845
              L+   C  K +K  +S                                          
Sbjct: 783  ALLNLKNC--KKLKHLSSDLYKLKCLQELILSGCSQLEVFPEIKEDMESLEILLMDDTSI 842

Query: 846  --MSWLLDRSNNHTTSFVGLQ---------LPPLGGIQKLEALYLDNCNLLELP-ALDKL 905
              M  ++  SN  T S  G           +PP  G  +L  LYL  C+L +LP  +  L
Sbjct: 843  TEMPKMMHLSNIKTFSLCGTSSHVSVSMFFMPPTLGCSRLTDLYLSRCSLYKLPDNIGGL 902

Query: 906  PSLESLDLRGNNFENLPQTIFSNLSSLKYLKLSYCRRLRSLPELPPNLRLLKADECTSLE 965
             SL+SL L GNN ENLP++ F+ L++LK+  L +C+ L+SLP LP NL+ L A EC SLE
Sbjct: 903  SSLQSLCLSGNNIENLPES-FNQLNNLKWFDLKFCKMLKSLPVLPQNLQYLDAHECESLE 962

Query: 966  SFMSSVTLYKGIGRWNAKMYQFGNCLKLKD--QCNNILESMIKGIHLSEGIVQNLYDQGY 1025
            +  + +T    +G     M+ F NC KL    Q + +  + IK   ++    +  Y +G+
Sbjct: 963  TLANPLTPLT-VGERIHSMFIFSNCYKLNQDAQASLVGHARIKSQLMANASAKRYY-RGF 1022

Query: 1026 CCRDGLNFSPWVAFPGSEIPEWFSNQAVGKATTLHLPPRCLD-KSLGFALCFILSPNGTF 1061
                 +     + +P +EIP WF +Q +G++  + LPP   D   +G AL  ++S    F
Sbjct: 1023 VPEPLVG----ICYPATEIPSWFCHQRLGRSLEIPLPPHWCDINFVGLALSVVVS----F 1082

BLAST of Lcy08g016420 vs. TAIR 10
Match: AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 511.9 bits (1317), Expect = 1.2e-144
Identity = 371/1172 (31.66%), Postives = 558/1172 (47.61%), Query Frame = 0

Query: 1    MAESSSSQPISIWIQPQYDVFVSFRGEDVRNNFVSHLQAALKRKGITTFSDEDQLKRGEE 60
            +  SSSS   ++W   + DVFVSFRGEDVR  FVSHL     R GI  F D+  L+RG+ 
Sbjct: 4    LPSSSSSSSSTVW---KTDVFVSFRGEDVRKTFVSHLFCEFDRMGIKAFRDDLDLQRGKS 63

Query: 61   ISPALVEAIQQSSISVVVFSQNYASSKWCLEELSKIVERRDMDGQMVMPVFYHVHPSHVR 120
            ISP L++AI+ S  ++VV S+NYA+S WCL+EL KI+E    +   ++P+FY V PS VR
Sbjct: 64   ISPELIDAIKGSRFAIVVVSRNYAASSWCLDELLKIME---CNKDTIVPIFYEVDPSDVR 123

Query: 121  NQTGSYAEAFRKHEETLEDKKDRIQTWKDALKQIANLSGFHSANWRLESQMIDKITQEIL 180
             Q GS+ E    H +     K+++  WK+ALK++A +SG  S NW  +S++I KI ++I 
Sbjct: 124  RQRGSFGEDVESHSD-----KEKVGKWKEALKKLAAISGEDSRNWD-DSKLIKKIVKDIS 183

Query: 181  KKLDRLTLDDVDGLVGIEHRVERIESSLCTDSNDVRILGIWGMGGIGKTTVARTLFHKIK 240
             KL   + DD  GL+G+   ++ ++S +     DVR+LGIWGMGG+GKTT+A+ L++++ 
Sbjct: 184  DKLVSTSWDDSKGLIGMSSHMDFLQSMISIVDKDVRMLGIWGMGGVGKTTIAKYLYNQLS 243

Query: 241  NQFDGCCFLENIRENSGKNGHGLPDLRKKLLSQVMEEAHLPMDIS---REFLKKRMCGKK 300
             QF   CF+EN++E    N +G+  L+ + L ++ +E       S      +K+R   K 
Sbjct: 244  GQFQVHCFMENVKEVC--NRYGVRRLQVEFLCRMFQERDKEAWSSVSCCNIIKERFRHKM 303

Query: 301  VLIILDDIDDMEQLENLAGRHNWFGVGSRIVITTRNRQLLTEVDEVYDYAKEQLTYEEAL 360
            V I+LDD+D  EQL  L     WFG GSRI++TTR+R LL        Y  + L  +EAL
Sbjct: 304  VFIVLDDVDRSEQLNELVKETGWFGPGSRIIVTTRDRHLLLSHGINLVYKVKCLPKKEAL 363

Query: 361  LLFSHHAFNSS-HPTEAYLELSRQAINYACGIPLALKILGSCLRTKDEKTWESALARLER 420
             LF ++AF         + ELS QA+NYA G+PLAL++LGS L  + +  WES LARL+ 
Sbjct: 364  QLFCNYAFREEIILPHGFEELSVQAVNYASGLPLALRVLGSFLYRRSQIEWESTLARLKT 423

Query: 421  FSNDKIVKRVLEISFEGLDWSEKNILLDVACFFKGEDVKFATSIWNVCGFFPDYGISVLR 480
            + +  I++ VL +S++GLD  EK I L ++CF+  + V +   + ++CG+  + GI++L 
Sbjct: 424  YPHSDIME-VLRVSYDGLDEQEKAIFLYISCFYNMKQVDYVRKLLDLCGYAAEIGITILT 483

Query: 481  DKCLISLLCNSQLWMHDLIQEMCKAIVRKENT----------DP---------------- 540
            +K LI +  N  + +HDL+++M + +VR++            DP                
Sbjct: 484  EKSLI-VESNGCVKIHDLLEQMGRELVRQQAVNNPAQRLLLWDPEDICHLLSENSGTQLV 543

Query: 541  ---------------GKDAFVKMKNLRLLKFFSSKLDASMRIRLPRGLTMLPDSLRYLFW 600
                              AF  + NL+LL F+    D   R+ LP GL+ LP  LRYL W
Sbjct: 544  EGISLNLSEISEVFASDRAFEGLSNLKLLNFYDLSFDGETRVHLPNGLSYLPRKLRYLRW 603

Query: 601  DGYPNKFLPSKFNAQNLLGLSLRQGCINQLW----------------------------- 660
            DGYP K +PS+F  + L+ L +    + +LW                             
Sbjct: 604  DGYPLKTMPSRFFPEFLVELCMSNSNLEKLWDGIQPLRNLKKMDLSRCKYLVEVPDLSKA 663

Query: 661  ------------------------KEHSMFKHSS----------ICLKHLKRLDLSGCPN 720
                                    K  S F  ++          I LK L+ + +SGC +
Sbjct: 664  TNLEELNLSYCQSLVEVTPSIKNLKGLSCFYLTNCIQLKDIPIGIILKSLETVGMSGCSS 723

Query: 721  FSNFSAICDNMEHLYELSLEGTSASNMPSSIGNLTELLSLNLSYCKELVSLPDSICNLKR 780
              +F  I  N   LY   L  T    +PSSI  L+ L+ L++S C+ L +LP  + +L  
Sbjct: 724  LKHFPEISWNTRRLY---LSSTKIEELPSSISRLSCLVKLDMSDCQRLRTLPSYLGHLVS 783

Query: 781  LQRLDLNGCESLQHLPENIGKLESLWWLRADG---------------------TSIKNVP 840
            L+ L+L+GC  L++LP+ +  L SL  L   G                     TSI+ +P
Sbjct: 784  LKSLNLDGCRRLENLPDTLQNLTSLETLEVSGCLNVNEFPRVSTSIEVLRISETSIEEIP 843

Query: 841  SSIICLKKIGTLSFSGCR---------------------GKGIKRS-------TSHWMSW 900
            + I  L ++ +L  S  +                     G  +  S       T   + W
Sbjct: 844  ARICNLSQLRSLDISENKRLASLPVSISELRSLEKLKLSGCSVLESFPLEICQTMSCLRW 903

Query: 901  L-LDRSN-----NHTTSFVGLQL------------------------------------- 960
              LDR++      +  + V L++                                     
Sbjct: 904  FDLDRTSIKELPENIGNLVALEVLQASRTVIRRAPWSIARLTRLQVLAIGNSFFTPEGLL 963

Query: 961  ----PPLGGIQKLEALYLDNCNLLELP-ALDKLPSLESLDLRGNNFENLPQTIFSNLSSL 966
                PPL     L AL L N N+ E+P ++  L +L  LDL GNNFE +P +I   L+ L
Sbjct: 964  HSLCPPLSRFDDLRALSLSNMNMTEIPNSIGNLWNLLELDLSGNNFEFIPASI-KRLTRL 1023

BLAST of Lcy08g016420 vs. TAIR 10
Match: AT5G36930.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 505.0 bits (1299), Expect = 1.5e-142
Identity = 329/884 (37.22%), Postives = 500/884 (56.56%), Query Frame = 0

Query: 18  YDVFVSFRGEDVRNNFVSHLQAALKRKGITTFSDEDQLKRGEEISPALVEAIQQSSISVV 77
           YDVFVSFRG DVR NF+SHL  +L+R GI+TF D+ +L+RGE ISP L+ AI+ S I +V
Sbjct: 14  YDVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKILIV 73

Query: 78  VFSQNYASSKWCLEELSKIVE-RRDMDGQMVMPVFYHVHPSHVRNQTGSYAEAFRKHEET 137
           V +++YASS WCL+EL  I++  ++    MV P+F +V PS +R Q GSYA++F KH+ +
Sbjct: 74  VLTKDYASSAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKHKNS 133

Query: 138 LEDKKDRIQTWKDALKQIANLSGFHSANWRLESQMIDKITQEILKKLDRLTLDDVDGLVG 197
                ++++ W++AL ++AN+SG+   N R E++ I  IT+EILK+L    L      VG
Sbjct: 134 --HPLNKLKDWREALTKVANISGWDIKN-RNEAECIADITREILKRLPCQYLHVPSYAVG 193

Query: 198 IEHRVERIESSLCTDSNDVRILGIWGMGGIGKTTVARTLFHKIKNQFDGCCFLENIRENS 257
           +  R++ I S L   S+ VR++ I+GMGGIGKTT+A+  F++  + F+G  FLEN RE S
Sbjct: 194 LRSRLQHISSLLSIGSDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLENFREYS 253

Query: 258 GKNGHGLPDLRKKLLSQVMEEAHLPMDISREFLKKRMCGKKVLIILDDIDDMEQLENLAG 317
            K   G   L+ +LLS ++    +        +K+R   K+VL+++DD+DD+ QL + A 
Sbjct: 254 -KKPEGRTHLQHQLLSDILRRNDIEFKGLDHAVKERFRSKRVLLVVDDVDDVHQLNSAAI 313

Query: 318 RHNWFGVGSRIVITTRNRQLLTEVDEVYDYAKEQLTYEEALLLFSHHAFNSSHPTEAYLE 377
             + FG GSRI+ITTRN  LL ++     Y+ ++L  +E+L LFS HAF +S P + +L+
Sbjct: 314 DRDCFGHGSRIIITTRNMHLLKQLRAEGSYSPKELDGDESLELFSWHAFRTSEPPKEFLQ 373

Query: 378 LSRQAINYACGIPLALKILGSCLRTKDEKTWESALARLERFSNDKIVKRVLEISFEGLDW 437
            S + + Y  G+PLA+++LG+ L  +  + WES L  L+R  ND I  + L+ISF  L  
Sbjct: 374 HSEEVVTYCAGLPLAVEVLGAFLIERSIREWESTLKLLKRIPNDNIQAK-LQISFNALTI 433

Query: 438 SEKNILLDVACFFKGEDVKFATSIWNVCGFFPDYGISVLRDKCLISLLCNSQLWMHDLIQ 497
            +K++ LD+ACFF G D  +   I + C  +PD  +S+L ++CLI++  N+ + MHDL++
Sbjct: 434 EQKDVFLDIACFFIGVDSYYVACILDGCNLYPDIVLSLLMERCLITISGNN-IMMHDLLR 493

Query: 498 EMCKAIVR-------------------------KENTDPGK----------------DAF 557
           +M + IVR                         K  T+  +                +AF
Sbjct: 494 DMGRQIVREISPKKCGERSRLWSHNDVVGVLKKKSGTNAIEGLSLKADVMDFQYFEVEAF 553

Query: 558 VKMKNLRLLKFFSSKLDASMRIRLPRGLTMLPDSLRYLFWDGYPNKFLPSKFNAQNLLGL 617
            KM+ LRLL+     L+ S            P  LR+L W G+  +  P   + ++L  L
Sbjct: 554 AKMQELRLLELRYVDLNGSYE--------HFPKDLRWLCWHGFSLECFPINLSLESLAAL 613

Query: 618 SLRQGCINQLWKEHSMFKHSSICLKHLKRLDLS------GCPNFSNFSAICDNMEHLYEL 677
            L+   + + WK  S  + +++    +K LDLS        P+FS F     N+E L  +
Sbjct: 614 DLQYSNLKRFWKAQSPPQPANM----VKYLDLSHSVYLRETPDFSYF----PNVEKLILI 673

Query: 678 SLEGTSASNMPSSIGNL-TELLSLNLSYCKELVSLPDSICNLKRLQRLDLNGCESLQHLP 737
           + +  S   +  SIG L  +L+ LNLS C EL  LP+ I  LK L+ L L+ C  L+ L 
Sbjct: 674 NCK--SLVLVHKSIGILDKKLVLLNLSSCIELDVLPEEIYKLKSLESLFLSNCSKLERLD 733

Query: 738 ENIGKLESLWWLRADGTSIKNVPSSIICLKKIGTLSFSGCRGKGIKRSTSHWMSWLLDRS 797
           + +G+LESL  L AD T+++ +PS+I  LKK+  LS +GC+G          +S  +D  
Sbjct: 734 DALGELESLTTLLADFTALREIPSTINQLKKLKRLSLNGCKG---------LLSDDIDNL 793

Query: 798 NNHTTSFVGLQLP-PLGGIQKLEALYLDNCNLLE--LPA-LDKLPSLESLDLRGNNFENL 849
            +  +  V L  P  L G+  +  L L  CNL +  +P  +  L  L  LDLRGN+F NL
Sbjct: 794 YSEKSHSVSLLRPVSLSGLTYMRILSLGYCNLSDELIPEDIGSLSFLRDLDLRGNSFCNL 853

BLAST of Lcy08g016420 vs. TAIR 10
Match: AT5G36930.2 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 505.0 bits (1299), Expect = 1.5e-142
Identity = 329/884 (37.22%), Postives = 500/884 (56.56%), Query Frame = 0

Query: 18  YDVFVSFRGEDVRNNFVSHLQAALKRKGITTFSDEDQLKRGEEISPALVEAIQQSSISVV 77
           YDVFVSFRG DVR NF+SHL  +L+R GI+TF D+ +L+RGE ISP L+ AI+ S I +V
Sbjct: 17  YDVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKILIV 76

Query: 78  VFSQNYASSKWCLEELSKIVE-RRDMDGQMVMPVFYHVHPSHVRNQTGSYAEAFRKHEET 137
           V +++YASS WCL+EL  I++  ++    MV P+F +V PS +R Q GSYA++F KH+ +
Sbjct: 77  VLTKDYASSAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKHKNS 136

Query: 138 LEDKKDRIQTWKDALKQIANLSGFHSANWRLESQMIDKITQEILKKLDRLTLDDVDGLVG 197
                ++++ W++AL ++AN+SG+   N R E++ I  IT+EILK+L    L      VG
Sbjct: 137 --HPLNKLKDWREALTKVANISGWDIKN-RNEAECIADITREILKRLPCQYLHVPSYAVG 196

Query: 198 IEHRVERIESSLCTDSNDVRILGIWGMGGIGKTTVARTLFHKIKNQFDGCCFLENIRENS 257
           +  R++ I S L   S+ VR++ I+GMGGIGKTT+A+  F++  + F+G  FLEN RE S
Sbjct: 197 LRSRLQHISSLLSIGSDGVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLENFREYS 256

Query: 258 GKNGHGLPDLRKKLLSQVMEEAHLPMDISREFLKKRMCGKKVLIILDDIDDMEQLENLAG 317
            K   G   L+ +LLS ++    +        +K+R   K+VL+++DD+DD+ QL + A 
Sbjct: 257 -KKPEGRTHLQHQLLSDILRRNDIEFKGLDHAVKERFRSKRVLLVVDDVDDVHQLNSAAI 316

Query: 318 RHNWFGVGSRIVITTRNRQLLTEVDEVYDYAKEQLTYEEALLLFSHHAFNSSHPTEAYLE 377
             + FG GSRI+ITTRN  LL ++     Y+ ++L  +E+L LFS HAF +S P + +L+
Sbjct: 317 DRDCFGHGSRIIITTRNMHLLKQLRAEGSYSPKELDGDESLELFSWHAFRTSEPPKEFLQ 376

Query: 378 LSRQAINYACGIPLALKILGSCLRTKDEKTWESALARLERFSNDKIVKRVLEISFEGLDW 437
            S + + Y  G+PLA+++LG+ L  +  + WES L  L+R  ND I  + L+ISF  L  
Sbjct: 377 HSEEVVTYCAGLPLAVEVLGAFLIERSIREWESTLKLLKRIPNDNIQAK-LQISFNALTI 436

Query: 438 SEKNILLDVACFFKGEDVKFATSIWNVCGFFPDYGISVLRDKCLISLLCNSQLWMHDLIQ 497
            +K++ LD+ACFF G D  +   I + C  +PD  +S+L ++CLI++  N+ + MHDL++
Sbjct: 437 EQKDVFLDIACFFIGVDSYYVACILDGCNLYPDIVLSLLMERCLITISGNN-IMMHDLLR 496

Query: 498 EMCKAIVR-------------------------KENTDPGK----------------DAF 557
           +M + IVR                         K  T+  +                +AF
Sbjct: 497 DMGRQIVREISPKKCGERSRLWSHNDVVGVLKKKSGTNAIEGLSLKADVMDFQYFEVEAF 556

Query: 558 VKMKNLRLLKFFSSKLDASMRIRLPRGLTMLPDSLRYLFWDGYPNKFLPSKFNAQNLLGL 617
            KM+ LRLL+     L+ S            P  LR+L W G+  +  P   + ++L  L
Sbjct: 557 AKMQELRLLELRYVDLNGSYE--------HFPKDLRWLCWHGFSLECFPINLSLESLAAL 616

Query: 618 SLRQGCINQLWKEHSMFKHSSICLKHLKRLDLS------GCPNFSNFSAICDNMEHLYEL 677
            L+   + + WK  S  + +++    +K LDLS        P+FS F     N+E L  +
Sbjct: 617 DLQYSNLKRFWKAQSPPQPANM----VKYLDLSHSVYLRETPDFSYF----PNVEKLILI 676

Query: 678 SLEGTSASNMPSSIGNL-TELLSLNLSYCKELVSLPDSICNLKRLQRLDLNGCESLQHLP 737
           + +  S   +  SIG L  +L+ LNLS C EL  LP+ I  LK L+ L L+ C  L+ L 
Sbjct: 677 NCK--SLVLVHKSIGILDKKLVLLNLSSCIELDVLPEEIYKLKSLESLFLSNCSKLERLD 736

Query: 738 ENIGKLESLWWLRADGTSIKNVPSSIICLKKIGTLSFSGCRGKGIKRSTSHWMSWLLDRS 797
           + +G+LESL  L AD T+++ +PS+I  LKK+  LS +GC+G          +S  +D  
Sbjct: 737 DALGELESLTTLLADFTALREIPSTINQLKKLKRLSLNGCKG---------LLSDDIDNL 796

Query: 798 NNHTTSFVGLQLP-PLGGIQKLEALYLDNCNLLE--LPA-LDKLPSLESLDLRGNNFENL 849
            +  +  V L  P  L G+  +  L L  CNL +  +P  +  L  L  LDLRGN+F NL
Sbjct: 797 YSEKSHSVSLLRPVSLSGLTYMRILSLGYCNLSDELIPEDIGSLSFLRDLDLRGNSFCNL 856

BLAST of Lcy08g016420 vs. TAIR 10
Match: AT5G44510.1 (target of AVRB operation1 )

HSP 1 Score: 473.4 bits (1217), Expect = 4.8e-133
Identity = 344/1064 (32.33%), Postives = 540/1064 (50.75%), Query Frame = 0

Query: 4    SSSSQPISI---WIQPQYDVFVSFRGEDVRNNFVSHLQAALKRKGITTFSDEDQLKRGEE 63
            SSSS P S+   W+ P   VF+SFRGEDVR   +SH+Q   +R GIT F D +++KRG  
Sbjct: 26   SSSSPPSSLSQNWLHP---VFLSFRGEDVRKGLLSHIQKEFQRNGITPFID-NEMKRGGS 85

Query: 64   ISPALVEAIQQSSISVVVFSQNYASSKWCLEELSKIVERRDMDGQMVMPVFYHVHPSHVR 123
            I P L++AI+ S I++++ S+NY SSKWCL+EL +I++ R+  GQ VM VFY V PS VR
Sbjct: 86   IGPELLQAIRGSKIAIILLSRNYGSSKWCLDELVEIMKCREELGQTVMTVFYDVDPSDVR 145

Query: 124  NQTGSYAEAFRKHEETLEDKKDRIQTWKDALKQIANLSGFHSANWRLESQMIDKITQEIL 183
             Q G + + F+K    +   ++ +Q WK AL   AN+ G  S NW  E+ MI KI++++ 
Sbjct: 146  KQKGDFGKVFKK--TCVGRPEEMVQRWKQALTSAANILGEDSRNWENEADMIIKISKDVS 205

Query: 184  KKLDRLTLDDVDGLVGIEHRVERIESSLCTDSNDVRILGIWGMGGIGKTTVARTLFHKIK 243
              L      D D  VGIE     I S L  D  +VR++GIWG  GIGKTT++R L++K+ 
Sbjct: 206  DVLSFTPSKDFDEFVGIEAHTTEITSLLQLDLEEVRMIGIWGPAGIGKTTISRVLYNKLF 265

Query: 244  NQFDGCCFLENIRENSGKNGH----GLPDLRKKLLSQVMEEAHL---PMDISREFLKKRM 303
            +QF     ++NI+    +  H        L+K+LLSQ++ +  +    + +++E LK   
Sbjct: 266  HQFQLGAIIDNIKVRYPRPCHDEYSAKLQLQKELLSQMINQKDMVVPHLGVAQERLK--- 325

Query: 304  CGKKVLIILDDIDDMEQLENLAGRHNWFGVGSRIVITTRNRQLLTEVDEVYDYAKEQLTY 363
              KKVL++LDD+D + QL+ +A    WFG+GSRI++ T++ +LL      Y Y  +  T 
Sbjct: 326  -DKKVLLVLDDVDGLVQLDAMAKDVQWFGLGSRIIVVTQDLKLLKAHGIKYIYKVDFPTS 385

Query: 364  EEALLLFSHHAFNSSHPTEAYLELSRQAINYACGIPLALKILGSCLRTKDEKTWESALAR 423
            +EAL +F  +AF    P   + +++R     A  +PL L+++GS LR   ++ W  ++ R
Sbjct: 386  DEALEIFCMYAFGEKSPKVGFEQIARTVTTLAGKLPLGLRVMGSYLRRMSKQEWAKSIPR 445

Query: 424  LERFSNDKIVKRVLEISFEGLDWSEKNILLDVACFFKGEDVKFATSIWNVCGFFPDYGIS 483
            L R S D  ++ VL+ S+  L   EK++ L + CFF+ E ++               G+ 
Sbjct: 446  L-RTSLDDDIESVLKFSYNSLAEQEKDLFLHITCFFRRERIETLEVFLAKKSVDMRQGLQ 505

Query: 484  VLRDKCLISLLCNSQLWMHDLIQEMCKAIVRKENT-DPGK-------------------- 543
            +L DK L+SL   + + MH+L+ ++   IVRK++   PGK                    
Sbjct: 506  ILADKSLLSLNLGN-IEMHNLLVQLGLDIVRKQSIHKPGKRQFLVDTEDICEVLTDDTGT 565

Query: 544  ----------------------DAFVKMKNLRLLKFFSSKLDASMRI-RLPRGLTMLPDS 603
                                   AF +M NL+ L+F     D    I  LP+GL+ +   
Sbjct: 566  RTLIGIDLELSGVIEGVINISERAFERMCNLQFLRFHHPYGDRCHDILYLPQGLSHISRK 625

Query: 604  LRYLFWDGYPNKFLPSKFNAQNLLGLSLRQGCINQLWKEHSMFKH-----SSICLK---- 663
            LR L W+ YP   LP KFN + L+ +++R   + +LW  +   ++      S C+     
Sbjct: 626  LRLLHWERYPLTCLPPKFNPEFLVKINMRDSMLEKLWDGNEPIRNLKWMDLSFCVNLKEL 685

Query: 664  -------HLKRLDLSGCPNFSNFSAICDNMEHLYELSL-EGTSASNMPSSIGNLTELLSL 723
                   +L+ L L  C +     +   N  +L EL L + +S   +PSSIGNLT L  L
Sbjct: 686  PDFSTATNLQELRLINCLSLVELPSSIGNATNLLELDLIDCSSLVKLPSSIGNLTNLKKL 745

Query: 724  NLSYCKELVSLPDSICNLKRLQRLDLNGCESLQHLPENIGKLESLWWLRADG-TSIKNVP 783
             L+ C  LV LP S  N+  L+ L+L+GC SL  +P +IG + +L  + ADG +S+  +P
Sbjct: 746  FLNRCSSLVKLPSSFGNVTSLKELNLSGCSSLLEIPSSIGNIVNLKKVYADGCSSLVQLP 805

Query: 784  SSIICLKKIGTLSFSGCRGKGIKRSTSHWMSWLLDRSNNHTTSFVGLQLPPLGGIQKLEA 843
            SSI     +  L    C       S+   ++ L D + +   S V  +LP +G +  L++
Sbjct: 806  SSIGNNTNLKELHLLNCSSLMECPSSMLNLTRLEDLNLSGCLSLV--KLPSIGNVINLQS 865

Query: 844  LYLDNC-NLLELP-ALDKLPSLESLDLRG-NNFENLPQTIFSNLSSLKYLKLSYCRRLRS 903
            LYL +C +L+ELP  ++   +L++L L G +N   LP +I+ N+++L+ L L+ C  L+ 
Sbjct: 866  LYLSDCSSLMELPFTIENATNLDTLYLDGCSNLLELPSSIW-NITNLQSLYLNGCSSLKE 925

Query: 904  LPELPP---NLRLLKADECTSLESFMSSVTLYKGIGRWNAKMYQFGNCLKLKD----QCN 960
            LP L     NL+ L   +C+SL    SS+         N       NC  L +       
Sbjct: 926  LPSLVENAINLQSLSLMKCSSLVELPSSIWRIS-----NLSYLDVSNCSSLLELNLVSHP 985