Lcy08g012590 (gene) Sponge gourd (P93075) v1

BLAST of Lcy08g012590 vs. ExPASy Swiss-Prot
Match: O64474 (Putative cadmium/zinc-transporting ATPase HMA4 OS=Arabidopsis thaliana OX=3702 GN=HMA4 PE=1 SV=2)

HSP 1 Score: 959.9 bits (2480), Expect = 1.9e-278
Identity = 513/836 (61.36%), Postives = 650/836 (77.75%), Query Frame = 0

Query: 7   EKEKVMRENKLQRSHFDVLGLCCSSEVPLIENILKPLEGIKDISVIVPTRTLIVVHDSLL 66
           EK+KV    KLQ+S+FDVLG+CC+SEVP+IENILK L+G+K+ SVIVP+RT+IVVHDSLL
Sbjct: 9   EKKKV---KKLQKSYFDVLGICCTSEVPIIENILKSLDGVKEYSVIVPSRTVIVVHDSLL 68

Query: 67  ISQLQIVKALNEARLEANVQLNGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLQWLAV 126
           IS  QI KALNEARLEANV++NG+   K KWPSPFA+ SGLLL  SFLK+VY PL+WLAV
Sbjct: 69  ISPFQIAKALNEARLEANVRVNGETSFKNKWPSPFAVVSGLLLLLSFLKFVYSPLRWLAV 128

Query: 127 AAVAAGIFPILLKAVSAVRHLRIDVNILAIIAVVGTIAMNDYMEAGSIVFLFSIAEWLES 186
           AAVAAGI+PIL KA ++++  RID+NIL II V+ T+AM D+MEA ++VFLF+I++WLE+
Sbjct: 129 AAVAAGIYPILAKAFASIKRPRIDINILVIITVIATLAMQDFMEAAAVVFLFTISDWLET 188

Query: 187 RASHKANAAMSSLMSLAPQTATIAETGEIVDSKEVKLNTVLAVKAGEVIPIDGIVVEGKC 246
           RAS+KA + M SLMSLAPQ A IAETGE V+  EVK++TV+AVKAGE IPIDGIVV+G C
Sbjct: 189 RASYKATSVMQSLMSLAPQKAIIAETGEEVEVDEVKVDTVVAVKAGETIPIDGIVVDGNC 248

Query: 247 EVDEKTLTGESFPVAKQKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAELVEEAQNNK 306
           EVDEKTLTGE+FPV KQ+DS VWAGTINLNGYI V+TT +A DCVVAKMA+LVEEAQ++K
Sbjct: 249 EVDEKTLTGEAFPVPKQRDSTVWAGTINLNGYICVKTTSLAGDCVVAKMAKLVEEAQSSK 308

Query: 307 SKTQRFIDECAKYYTPAVLVISACLAAIPAALRVHNLSHWFHLALVVLVSACPCALILST 366
           +K+QR ID+C++YYTPA++++SAC+A +P  ++VHNL HWFHLALVVLVS CPC LILST
Sbjct: 309 TKSQRLIDKCSQYYTPAIILVSACVAIVPVIMKVHNLKHWFHLALVVLVSGCPCGLILST 368

Query: 367 PVAAFCALTKAAMAGVLIKGGDHLELLAKIKVMAFDKTGTITRGEFVVTDFRALCDNISL 426
           PVA FCALTKAA +G+LIK  D+L+ L+KIK++AFDKTGTITRGEF+V DF++L  +I+L
Sbjct: 369 PVATFCALTKAATSGLLIKSADYLDTLSKIKIVAFDKTGTITRGEFIVIDFKSLSRDINL 428

Query: 427 HTLLHWVSSIESKSSHPMAAALVNHGKFLSVDIKPENVEEFENFPGEGVRGKIDGNNIYI 486
            +LL+WVSS+ESKSSHPMAA +V++ K +SV+ +PE VE+++NFPGEG+ GKIDGN+I+I
Sbjct: 429 RSLLYWVSSVESKSSHPMAATIVDYAKSVSVEPRPEEVEDYQNFPGEGIYGKIDGNDIFI 488

Query: 487 GSRKIAARAGCPTAPSSDDDETKQGQTVGYVFCGTTLAGSFGLSDACRSGVREAIQEIKS 546
           G++KIA+RAGC T P  + D TK G+TVGYV+ G  LAG F LSDACRSGV +A+ E+KS
Sbjct: 489 GNKKIASRAGCSTVPEIEVD-TKGGKTVGYVYVGERLAGFFNLSDACRSGVSQAMAELKS 548

Query: 547 FGIKTAMLTGDCNAAAMHAQQQLGNALDVIHSELLPKEKANIIKRFKGDGPTAMVGDGLN 606
            GIKTAMLTGD  AAAMHAQ+QLGN LDV+H +LLP++K+ II+ FK +GPTAMVGDG+N
Sbjct: 549 LGIKTAMLTGDNQAAAMHAQEQLGNVLDVVHGDLLPEDKSRIIQEFKKEGPTAMVGDGVN 608

Query: 607 DTPALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHAKVVQNVILSI 666
           D PALATADIGISMG+SGSALAT+TGN+ILMSND+R+IP+A+KLA+++  KVV+NV LSI
Sbjct: 609 DAPALATADIGISMGISGSALATQTGNIILMSNDIRRIPQAVKLARRARRKVVENVCLSI 668

Query: 667 GTKTAILGLAFAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHRHGKKCCKSSKLPCFS 726
             K  IL LAFAGHPL+WAAVL DVGTCLLVI NSMLLLR       KKC ++S      
Sbjct: 669 ILKAGILALAFAGHPLIWAAVLVDVGTCLLVIFNSMLLLREKKKIGNKKCYRAS------ 728

Query: 727 TKHGKCNSSNGRSSHRHDHHHDDHRCHVVDDRSSSRENRVHKHCCSEKNNVPQQKENKFH 786
                 +  NGR     D +  D    ++   + S   +    CC +K N    +EN   
Sbjct: 729 -----TSKLNGRKLEGDDDYVVDLEAGLL---TKSGNGQCKSSCCGDKKN----QENVVM 788

Query: 787 HNPSNQCEKNQVEKESNGDSSRK-----VAESNCNCHSHHVAIDIHEDTDCERVEH 838
             PS++   +       GD   +     V +  C+  +     D+   + C++  H
Sbjct: 789 MKPSSKTSSDHSHPGCCGDKKEEKVKPLVKDGCCSEKTRKSEGDMVSLSSCKKSSH 822

BLAST of Lcy08g012590 vs. ExPASy Swiss-Prot
Match: Q9SZW4 (Cadmium/zinc-transporting ATPase HMA2 OS=Arabidopsis thaliana OX=3702 GN=HMA2 PE=2 SV=1)

HSP 1 Score: 957.2 bits (2473), Expect = 1.2e-277
Identity = 516/850 (60.71%), Postives = 648/850 (76.24%), Query Frame = 0

Query: 12  MRENKLQRSHFDVLGLCCSSEVPLIENILKPLEGIKDISVIVPTRTLIVVHDSLLISQLQ 71
           M   K+ +S+FDVLG+CC+SEVPLIENIL  ++G+K+ SVIVP+RT+IVVHD+L++SQ Q
Sbjct: 1   MASKKMTKSYFDVLGICCTSEVPLIENILNSMDGVKEFSVIVPSRTVIVVHDTLILSQFQ 60

Query: 72  IVKALNEARLEANVQLNGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLQWLAVAAVAA 131
           IVKALN+A+LEANV++ G+   K KWPSPFA+ SG+LL  SF KY+Y P +WLAVAAV A
Sbjct: 61  IVKALNQAQLEANVRVTGETNFKNKWPSPFAVVSGILLLLSFFKYLYSPFRWLAVAAVVA 120

Query: 132 GIFPILLKAVSAVRHLRIDVNILAIIAVVGTIAMNDYMEAGSIVFLFSIAEWLESRASHK 191
           GI+PIL KAV+++   RID+NIL ++ V  TI M DY EA  +VFLF+IAEWL+SRAS+K
Sbjct: 121 GIYPILAKAVASLARFRIDINILVVVTVGATIGMQDYTEAAVVVFLFTIAEWLQSRASYK 180

Query: 192 ANAAMSSLMSLAPQTATIAETGEIVDSKEVKLNTVLAVKAGEVIPIDGIVVEGKCEVDEK 251
           A+A M SLMSLAPQ A IAETGE V+  E+K NTV+AVKAGE IPIDG+VV+G CEVDEK
Sbjct: 181 ASAVMQSLMSLAPQKAVIAETGEEVEVDELKTNTVIAVKAGETIPIDGVVVDGNCEVDEK 240

Query: 252 TLTGESFPVAKQKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAELVEEAQNNKSKTQR 311
           TLTGE+FPV K KDS VWAGTINLNGYI+V TT +AEDCVVAKMA+LVEEAQN+K++TQR
Sbjct: 241 TLTGEAFPVPKLKDSTVWAGTINLNGYITVNTTALAEDCVVAKMAKLVEEAQNSKTETQR 300

Query: 312 FIDECAKYYTPAVLVISACLAAIPAALRVHNLSHWFHLALVVLVSACPCALILSTPVAAF 371
           FID+C+KYYTPA+++IS C  AIP AL+VHNL HW HLALVVLVSACPC LILSTPVA F
Sbjct: 301 FIDKCSKYYTPAIILISICFVAIPFALKVHNLKHWVHLALVVLVSACPCGLILSTPVATF 360

Query: 372 CALTKAAMAGVLIKGGDHLELLAKIKVMAFDKTGTITRGEFVVTDFRALCDNISLHTLLH 431
           CALTKAA +G+LIKG D+LE LAKIK++AFDKTGTITRGEF+V DF++L ++ISL +LL+
Sbjct: 361 CALTKAATSGLLIKGADYLETLAKIKIVAFDKTGTITRGEFIVMDFQSLSEDISLQSLLY 420

Query: 432 WVSSIESKSSHPMAAALVNHGKFLSVDIKPENVEEFENFPGEGVRGKIDGNNIYIGSRKI 491
           WVSS ESKSSHPMAAA+V++ + +SV+ KPE VE+++NFPGEG+ GKIDG  +YIG+++I
Sbjct: 421 WVSSTESKSSHPMAAAVVDYARSVSVEPKPEAVEDYQNFPGEGIYGKIDGKEVYIGNKRI 480

Query: 492 AARAGCPTAPSSDDDETKQGQTVGYVFCGTTLAGSFGLSDACRSGVREAIQEIKSFGIKT 551
           A+RAGC + P  D D TK G+T+GYV+ G TLAG F LSDACRSGV +A++E+KS GIK 
Sbjct: 481 ASRAGCLSVPDIDVD-TKGGKTIGYVYVGETLAGVFNLSDACRSGVAQAMKELKSLGIKI 540

Query: 552 AMLTGDCNAAAMHAQQQLGNALDVIHSELLPKEKANIIKRFK-GDGPTAMVGDGLNDTPA 611
           AMLTGD +AAAMHAQ+QLGNA+D++ +ELLP++K+ IIK+ K  +GPTAMVGDGLND PA
Sbjct: 541 AMLTGDNHAAAMHAQEQLGNAMDIVRAELLPEDKSEIIKQLKREEGPTAMVGDGLNDAPA 600

Query: 612 LATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHAKVVQNVILSIGTKT 671
           LATADIGISMGVSGSALATETGN+ILMSND+R+IP+AIKLAK++  KVV+NV++SI  K 
Sbjct: 601 LATADIGISMGVSGSALATETGNIILMSNDIRRIPQAIKLAKRAKRKVVENVVISITMKG 660

Query: 672 AILGLAFAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHRHGKKCCKSSK--------- 731
           AIL LAFAGHPL+WAAVLADVGTCLLVILNSMLLL    H+ G KC + S          
Sbjct: 661 AILALAFAGHPLIWAAVLADVGTCLLVILNSMLLL-SDKHKTGNKCYRESSSSSVLIAEK 720

Query: 732 -------------LPCFSTKH---GKCNSSNGRSSHRHDHHHDDH-RCHVVDDRSSSREN 791
                        LP  S KH   G C +     + +      DH      + +      
Sbjct: 721 LEGDAAGDMEAGLLPKISDKHCKPGCCGTKTQEKAMKPAKASSDHSHSGCCETKQKDNVT 780

Query: 792 RVHKHCCSEKNNVPQQKENKFHHNPSNQCEKNQVEKESNGDSSRKVAESNC------NCH 829
            V K CC+E  ++    ++    + S Q  +++V+ + +  +     +S C        H
Sbjct: 781 VVKKSCCAEPVDLGHGHDSGCCGDKSQQPHQHEVQVQQSCHNKPSGLDSGCCGGKSQQPH 840

BLAST of Lcy08g012590 vs. ExPASy Swiss-Prot
Match: P0CW78 (Cadmium/zinc-transporting ATPase HMA3 OS=Arabidopsis thaliana OX=3702 GN=HMA3 PE=1 SV=1)

HSP 1 Score: 899.8 bits (2324), Expect = 2.3e-260
Identity = 463/716 (64.66%), Postives = 579/716 (80.87%), Query Frame = 0

Query: 6   MEKEKVMRENKLQRSHFDVLGLCCSSEVPLIENILKPLEGIKDISVIVPTRTLIVVHDSL 65
           M + +  ++  LQ S+FDV+G+CCSSEV ++ N+L+ ++G+K+ SVIVP+RT+IVVHD+ 
Sbjct: 1   MAEGEESKKMNLQTSYFDVVGICCSSEVSIVGNVLRQVDGVKEFSVIVPSRTVIVVHDTF 60

Query: 66  LISQLQIVKALNEARLEANVQLNGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLQWLA 125
           LIS LQIVKALN+ARLEA+V+  G+   K +WPSPFAI SG+LL  SF KY Y PL+WLA
Sbjct: 61  LISPLQIVKALNQARLEASVRPYGETSLKSQWPSPFAIVSGVLLVLSFFKYFYSPLEWLA 120

Query: 126 VAAVAAGIFPILLKAVSAVRHLRIDVNILAIIAVVGTIAMNDYMEAGSIVFLFSIAEWLE 185
           + AV AG+FPIL KAV++V   R+D+N L +IAV+ T+ M D+ EA +IVFLFS+A+WLE
Sbjct: 121 IVAVVAGVFPILAKAVASVTRFRLDINALTLIAVIATLCMQDFTEAATIVFLFSVADWLE 180

Query: 186 SRASHKANAAMSSLMSLAPQTATIAETGEIVDSKEVKLNTVLAVKAGEVIPIDGIVVEGK 245
           S A+HKA+  MSSLMSLAP+ A IA+TG  VD  EV +NTV++VKAGE IPIDG+VV+G 
Sbjct: 181 SSAAHKASIVMSSLMSLAPRKAVIADTGLEVDVDEVGINTVVSVKAGESIPIDGVVVDGS 240

Query: 246 CEVDEKTLTGESFPVAKQKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAELVEEAQNN 305
           C+VDEKTLTGESFPV+KQ++S V A TINLNGYI V+TT +A DCVVAKM +LVEEAQ +
Sbjct: 241 CDVDEKTLTGESFPVSKQRESTVMAATINLNGYIKVKTTALARDCVVAKMTKLVEEAQKS 300

Query: 306 KSKTQRFIDECAKYYTPAVLVISACLAAIPAALRVHNLSHWFHLALVVLVSACPCALILS 365
           ++KTQRFID+C++YYTPAV+V +AC A IP  L+V +LSHWFHLALVVLVS CPC LILS
Sbjct: 301 QTKTQRFIDKCSRYYTPAVVVSAACFAVIPVLLKVQDLSHWFHLALVVLVSGCPCGLILS 360

Query: 366 TPVAAFCALTKAAMAGVLIKGGDHLELLAKIKVMAFDKTGTITRGEFVVTDFRALCDNIS 425
           TPVA FCALTKAA +G LIK GD LE LAKIK++AFDKTGTIT+ EF+V+DFR+L  +I+
Sbjct: 361 TPVATFCALTKAATSGFLIKTGDCLETLAKIKIVAFDKTGTITKAEFMVSDFRSLSPSIN 420

Query: 426 LHTLLHWVSSIESKSSHPMAAALVNHGKFLSVDIKPENVEEFENFPGEGVRGKIDGNNIY 485
           LH LL+WVSSIE KSSHPMAAAL+++   +SV+ KP+ VE F+NFPGEGV G+IDG +IY
Sbjct: 421 LHKLLNWVSSIECKSSHPMAAALIDYAISVSVEPKPDIVENFQNFPGEGVYGRIDGQDIY 480

Query: 486 IGSRKIAARAGCPTAPSSDDDET-KQGQTVGYVFCGTTLAGSFGLSDACRSGVREAIQEI 545
           IG+++IA RAGC T    D + T K+G+T+GY++ G  L GSF L D CR GV +A++E+
Sbjct: 481 IGNKRIAQRAGCLTDNVPDIEATMKRGKTIGYIYMGAKLTGSFNLLDGCRYGVAQALKEL 540

Query: 546 KSFGIKTAMLTGDCNAAAMHAQQQLGNALDVIHSELLPKEKANIIKRFKGDGPTAMVGDG 605
           KS GI+TAMLTGD   AAM  Q+QL NALD++HSELLP++KA II  FK  GPT MVGDG
Sbjct: 541 KSLGIQTAMLTGDNQDAAMSTQEQLENALDIVHSELLPQDKARIIDDFKIQGPTMMVGDG 600

Query: 606 LNDTPALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHAKVVQNVIL 665
           LND PALA ADIGISMG+SGSALATETG++ILMSND+RKIPK ++LAK+SH KV++NV+L
Sbjct: 601 LNDAPALAKADIGISMGISGSALATETGDIILMSNDIRKIPKGMRLAKRSHKKVIENVVL 660

Query: 666 SIGTKTAILGLAFAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHRHGKKCCKSS 721
           S+  K AI+ L F G+PLVWAAVLAD GTCLLVILNSM+LLR  D R     C  S
Sbjct: 661 SVSIKGAIMVLGFVGYPLVWAAVLADAGTCLLVILNSMILLR--DEREAVSTCYRS 714

BLAST of Lcy08g012590 vs. ExPASy Swiss-Prot
Match: A3BF39 (Cadmium/zinc-transporting ATPase HMA2 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA2 PE=1 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 7.9e-253
Identity = 474/837 (56.63%), Postives = 616/837 (73.60%), Query Frame = 0

Query: 16  KLQRSHFDVLGLCCSSEVPLIENILKPLEGIKDISVIVPTRTLIVVHDSLLISQLQIVKA 75
           + Q+S+FDVLG+CC SEVPL+E +L+PLEG++ ++VIVP+RT+IVVHD   ISQ QIVKA
Sbjct: 7   RCQKSYFDVLGICCPSEVPLVEKLLQPLEGVQKVTVIVPSRTVIVVHDVDAISQSQIVKA 66

Query: 76  LNEARLEANVQLNGKGISK--KKWPSPFAIASGLLLTASFLKYVYHPLQWLAVAAVAAGI 135
           LN+ARLEA+V+  G G  K   KWPSP+ +  GLLL  S  ++ +HPL+W A+ A AAG+
Sbjct: 67  LNQARLEASVRAYGNGSEKITNKWPSPYVLLCGLLLVVSLFEHFWHPLKWFALVAAAAGL 126

Query: 136 FPILLKAVSAVRHLRIDVNILAIIAVVGTIAMNDYMEAGSIVFLFSIAEWLESRASHKAN 195
            PI+L++++A+R L +DVNIL +IAV G IA+ DY EAG IVFLF+ AEWLE+RASHKA 
Sbjct: 127 PPIVLRSIAAIRRLTLDVNILMLIAVAGAIALKDYSEAGFIVFLFTTAEWLETRASHKAT 186

Query: 196 AAMSSLMSLAPQTATIAETGEIVDSKEVKLNTVLAVKAGEVIPIDGIVVEGKCEVDEKTL 255
           A MS+LMS+APQ A +AETGE+V +++VK+NTV+AVKAGEVIPIDG+VV+G+ EVDE TL
Sbjct: 187 AGMSALMSMAPQKAILAETGEVVAARDVKVNTVIAVKAGEVIPIDGVVVDGRSEVDESTL 246

Query: 256 TGESFPVAKQKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAELVEEAQNNKSKTQRFI 315
           TGESFPV+KQ DS VWAGT+N++GYI+V+TT +A++  VAKMA LVEEAQN++S TQR I
Sbjct: 247 TGESFPVSKQPDSQVWAGTLNIDGYIAVRTTAMADNSAVAKMARLVEEAQNSRSSTQRLI 306

Query: 316 DECAKYYTPAVLVISACLAAIPAALRVHNLSHWFHLALVVLVSACPCALILSTPVAAFCA 375
           D CAKYYTPAV+V++  +AAIPA  + HNL HWF LALV+LVSACPCAL+LSTP+A FCA
Sbjct: 307 DTCAKYYTPAVVVMAGSVAAIPAIAKAHNLKHWFQLALVLLVSACPCALVLSTPIATFCA 366

Query: 376 LTKAAMAGVLIKGGDHLELLAKIKVMAFDKTGTITRGEFVVTDFRALCDNISLHTLLHWV 435
           L +AA  G+LIKGGD LE LA IKV AFDKTGTITRGEF V +F+ + + +SL  LL+WV
Sbjct: 367 LLRAARTGLLIKGGDVLESLASIKVAAFDKTGTITRGEFSVEEFQPVGERVSLQQLLYWV 426

Query: 436 SSIESKSSHPMAAALVNHGKFLSVDIKPENVEEFENFPGEGVRGKIDGNNIYIGSRKIAA 495
           SS+ES+SSHPMA+ LV++ +  SV+ K ENV EF+ +PGEG+ G+IDG  IYIG+++I +
Sbjct: 427 SSVESRSSHPMASVLVDYAQSKSVEPKSENVSEFQIYPGEGIYGEIDGAGIYIGNKRILS 486

Query: 496 RAGCPTAPSSDDDETKQGQTVGYVFCGTTLAGSFGLSDACRSGVREAIQEIKSFGIKTAM 555
           RA C T P   D    +G T+GYV C   L G F LSDACR+G  EAI+E++S GIK+ M
Sbjct: 487 RASCETVPDMKD---MKGVTIGYVACNNELIGVFTLSDACRTGSAEAIKELRSLGIKSVM 546

Query: 556 LTGDCNAAAMHAQQQLGNALDVIHSELLPKEKANIIKRFK-GDGPTAMVGDGLNDTPALA 615
           LTGD +AAA +AQ QLGN L  +H+ELLP++K  I+   K  DGPT MVGDG+ND PALA
Sbjct: 547 LTGDSSAAATYAQNQLGNILAEVHAELLPEDKVRIVGELKEKDGPTLMVGDGMNDAPALA 606

Query: 616 TADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHAKVVQNVILSIGTKTAI 675
            AD+G+SMGVSGSA+A ET +V LMSND+R+IPKA++LA+++H  ++ N+I S+ TK AI
Sbjct: 607 KADVGVSMGVSGSAVAMETSHVALMSNDIRRIPKAVRLARRTHRTIIVNIIFSVITKLAI 666

Query: 676 LGLAFAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHRHGKKCCK----SSKLPCFSTK 735
           +GLAFAGHPL+WAAVLADVGTCLLVI+ SMLLLR  D R  KKC      S K  C S+ 
Sbjct: 667 VGLAFAGHPLIWAAVLADVGTCLLVIMYSMLLLREKDSRKAKKCAASHHGSPKKCCSSSH 726

Query: 736 HGKCNSSNGRSSHRHDHHHDDHRCHVVDDRSSSRENRVHKHCCSEKNNVPQQKENKFHHN 795
           HG    S+ + +H   HH  D  C       S +E+ V K+ C + ++     E   H +
Sbjct: 727 HG----SHAKKNHGVSHHCSDGPC---KSMVSCKESSVAKNACHDHHHEHNHHEEPAHKH 786

Query: 796 PSNQ--CEKNQVEKESNGDSSRKVAESNCNCHSHH----VAIDIHEDT--DCERVEH 838
            SNQ  C  +     +  + S ++  +   CH  H       ++H+    DC   EH
Sbjct: 787 SSNQHGCHDHSHGHSNCKEPSNQLITNKHACHDGHNHCADTSNLHDTKKHDCHGHEH 833

BLAST of Lcy08g012590 vs. ExPASy Swiss-Prot
Match: Q8H384 (Cadmium/zinc-transporting ATPase HMA3 OS=Oryza sativa subsp. japonica OX=39947 GN=HMA3 PE=1 SV=1)

HSP 1 Score: 720.3 bits (1858), Expect = 2.5e-206
Identity = 390/766 (50.91%), Postives = 536/766 (69.97%), Query Frame = 0

Query: 16  KLQRSHFDVLGLCCSSEVPLIENILKPLEGIKDISVIVPTRTLIVVHDSLLISQLQIVKA 75
           K ++++ DVLG+CCS+EV L+E +L PL+G++ +SV+V +RT++V HD     +  IVKA
Sbjct: 40  KRKKTYLDVLGVCCSAEVALVERLLAPLDGVRVVSVVVASRTVVVEHDPAAAPESAIVKA 99

Query: 76  LNEARLEANVQLNGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLQWLAVAAVAAGIFP 135
           LN+A LEA+V+  G      +WPSP+ +ASG+LLTASF ++++ PLQ LAVAAV AG  P
Sbjct: 100 LNKAGLEASVRAYGSSGVVSRWPSPYIVASGVLLTASFFEWLFPPLQCLAVAAVVAGAPP 159

Query: 136 ILLKAVSAVRHLRIDVNILAIIAVVGTIAMNDYMEAGSIVFLFSIAEWLESRASHKANAA 195
           ++ +  +A   L +D+N+L +IAV G + + DY EAG+IVFLF+ AEWLE+ A  KA+A 
Sbjct: 160 MVRRGFAAASRLSLDINVLMLIAVAGALCLGDYTEAGAIVFLFTTAEWLETLACTKASAG 219

Query: 196 MSSLMSLAPQTATIAETGEIVDSKEVKLNTVLAVKAGEVIPIDGIVVEGKCEVDEKTLTG 255
           MSSLM + P  A IA TGE+V  ++V++  V+AV+AGE++P+DG+VV+G+ EVDE++LTG
Sbjct: 220 MSSLMGMLPVKAVIATTGEVVSVRDVRVGDVVAVRAGEIVPVDGVVVDGQSEVDERSLTG 279

Query: 256 ESFPVAKQKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAELVEEAQNNKSKTQRFIDE 315
           ESFPV KQ  S VWAGT+N +GYI+V+TT +AE+  VAKM  LVE AQN++SKTQR ID 
Sbjct: 280 ESFPVPKQPHSEVWAGTMNFDGYIAVRTTALAENSTVAKMERLVEAAQNSRSKTQRLIDS 339

Query: 316 CAKYYTPAVLVISACLAAIPAALRVHNLSHWFHLALVVLVSACPCALILSTPVAAFCALT 375
           CAKYYTPAV+V++A +A IPA L    L  W+ LALV+LVSACPCAL+LSTPVA+FCA+ 
Sbjct: 340 CAKYYTPAVVVVAAGVALIPALLGADGLEQWWKLALVMLVSACPCALVLSTPVASFCAML 399

Query: 376 KAAMAGVLIKGGDHLELLAKIKVMAFDKTGTITRGEFVVTDFRALCDN-ISLHTLLHWVS 435
           +AA  G+ IKGGD LE L +I+ +AFDKTGTITRGEF +  F  + D+ + +  LL+W++
Sbjct: 400 RAARMGIFIKGGDVLESLGEIRAVAFDKTGTITRGEFSIDSFHLVGDHKVEMDHLLYWIA 459

Query: 436 SIESKSSHPMAAALVNHGKFLSVDIKPENVEEFENFPGEGVRGKIDGNNIYIGSRKIAAR 495
           SIESKSSHPMAAALV + +  S+   PENV +F  +PGEG+ G+I G +IYIG+R+  AR
Sbjct: 460 SIESKSSHPMAAALVEYAQSKSIQPNPENVGDFRIYPGEGIYGEIHGKHIYIGNRRTLAR 519

Query: 496 AGCPTAPSSDDDETKQGQTVGYVFCGTTLAGSFGLSDACRSGVREAIQEIKSFGIKTAML 555
           A  P + + +  E  +G ++GYV C   LAG F LSD CR+G  EAI+E+ S GIK+ ML
Sbjct: 520 ASSPQS-TQEMGEMIKGVSIGYVICDGELAGVFSLSDDCRTGAAEAIRELGSLGIKSVML 579

Query: 556 TGDCNAAAMHAQQQLGNALDVIHSELLPKEKANIIKRFKGD-GPTAMVGDGLNDTPALAT 615
           TGD +AAA HAQ QLG  ++ +HSELLP++K  ++   K   GPT MVGDG+ND  ALA 
Sbjct: 580 TGDSSAAATHAQGQLGGVMEELHSELLPEDKVRLVSGLKARFGPTMMVGDGMNDAAALAA 639

Query: 616 ADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHAKVVQNVILSIGTKTAIL 675
           AD+G+SMG+SGSA A ET +  LMS+D+ ++P+A++L + +   +  NV  S+  K A+L
Sbjct: 640 ADVGVSMGISGSAAAMETSHATLMSSDVLRVPEAVRLGRCARRTIAVNVAGSVAVKAAVL 699

Query: 676 GLAFAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHRHGKKCCKSSKLPCFSTKHGKCN 735
            LA A  P++WAAVLADVGTCLLV+LNSM LLR  + + G K            + G C 
Sbjct: 700 ALAAAWRPVLWAAVLADVGTCLLVVLNSMTLLR-EEWKGGAK------------EDGACR 759

Query: 736 SSNGRSSHRHDHHHDDHRCHVVDDRSSSRENRVHKHCCSEKNNVPQ 780
           ++      R     D    +  D  ++ RE      CC +    P+
Sbjct: 760 ATARSLVMRSQLAADSQAPNAADAGAAGREQTNGCRCCPKPGMSPE 791

BLAST of Lcy08g012590 vs. ExPASy TrEMBL
Match: A0A6J1K4T4 (putative inactive cadmium/zinc-transporting ATPase HMA3 OS=Cucurbita maxima OX=3661 GN=LOC111492308 PE=3 SV=1)

HSP 1 Score: 1320.1 bits (3415), Expect = 0.0e+00
Identity = 702/843 (83.27%), Postives = 757/843 (89.80%), Query Frame = 0

Query: 1   MGEEAMEK-EKVMRENKLQRSHFDVLGLCCSSEVPLIENILKPLEGIKDISVIVPTRTLI 60
           +GE AMEK +KV RENK +RSHFDVLG+CCSSEVPL+ENILKPLEGIK ISVIVPTRTLI
Sbjct: 2   VGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLVENILKPLEGIKQISVIVPTRTLI 61

Query: 61  VVHDSLLISQLQIVKALNEARLEANVQLNGKGISKKKWPSPFAIASGLLLTASFLKYVYH 120
           VVHDSLL+SQLQIVKALNEARLEANVQ+NGKGISKKKWPSPFAIASGLLLTASFLKYVYH
Sbjct: 62  VVHDSLLVSQLQIVKALNEARLEANVQVNGKGISKKKWPSPFAIASGLLLTASFLKYVYH 121

Query: 121 PLQWLAVAAVAAGIFPILLKAVSAVRHLRIDVNILAIIAVVGTIAMNDYMEAGSIVFLFS 180
           PL+WLA+AAVAAGIFPILLKA+SAVRHLRID NILAI+AVVGTI+MNDYMEAGSIVFLFS
Sbjct: 122 PLRWLAIAAVAAGIFPILLKAISAVRHLRIDANILAIVAVVGTISMNDYMEAGSIVFLFS 181

Query: 181 IAEWLESRASHKANAAMSSLMSLAPQTATIAETGEIVDSKEVKLNTVLAVKAGEVIPIDG 240
           IAEWLESRASHKANAAMSSLMSLAPQ A IAETGE+V  KEV+L +VLAVK GEVIPIDG
Sbjct: 182 IAEWLESRASHKANAAMSSLMSLAPQKAIIAETGEVVAVKEVELKSVLAVKGGEVIPIDG 241

Query: 241 IVVEGKCEVDEKTLTGESFPVAKQKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAELV 300
           IVV+GKC+VDEKTLTGE+FPV K KDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAE V
Sbjct: 242 IVVDGKCDVDEKTLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFV 301

Query: 301 EEAQNNKSKTQRFIDECAKYYTPAVLVISACLAAIPAALRVHNLSHWFHLALVVLVSACP 360
           EEAQNNKSKTQRFIDECAKYYTPAV+VISAC+AAIPAA RVHNLSHWFHLALVVLVSACP
Sbjct: 302 EEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACP 361

Query: 361 CALILSTPVAAFCALTKAAMAGVLIKGGDHLELLAKIKVMAFDKTGTITRGEFVVTDFRA 420
           CALILSTPVAAFCALTKAAMAGVLIKG DHLE+LAKIKV+AFDKTGTITRGEFVVT+F+A
Sbjct: 362 CALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTNFQA 421

Query: 421 LCDNISLHTLLHWVSSIESKSSHPMAAALVNHGKFLSVDIKPENVEEFENFPGEGVRGKI 480
           L D+I+ HTLLHWVSSIESKSSHPMAAALVNH K LS+DIKPE VEEFENFPGEGVRGKI
Sbjct: 422 LRDDINFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGKI 481

Query: 481 DGNNIYIGSRKIAARAGCPTAPSSDDDETKQGQTVGYVFCGTTLAGSFGLSDACRSGVRE 540
           DG +IYIGS KIAARAGC +A    D+E KQGQT+GYVFCG    G+FGLSD+CRSG +E
Sbjct: 482 DGKDIYIGSTKIAARAGCSSAMKL-DEEMKQGQTLGYVFCGEMAVGAFGLSDSCRSGAKE 541

Query: 541 AIQEIKSFGIKTAMLTGDCNAAAMHAQQQLGNALDVIHSELLPKEKANIIKRFKGD-GPT 600
           A++++KS GIKTAMLTGDC+AAAMHAQQQL NA+DVIHSELLPKEKAN+IK FK D G T
Sbjct: 542 AMEQLKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANMIKEFKRDYGAT 601

Query: 601 AMVGDGLNDTPALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHAKV 660
           AMVGDGLNDTPALATADIGISMG+SGS LAT+TGNVILMSND+ KIP+AIKLA+KSHAKV
Sbjct: 602 AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 661

Query: 661 VQNVILSIGTKTAILGLAFAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHRHGKKCCK 720
           VQNVILSI TK AILGLA AGHPLVWAAVLADVGTCLLVILNSMLLLRG+DH+HG KCCK
Sbjct: 662 VQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 721

Query: 721 SSKLPCFSTKHGKCNSSNGRSS--HRHDHHHDDHRCHVVDDRSSSRENRVHKHCCSEKNN 780
           SSKL   STKHG+C+ SN R S  H H HHH  HRCHVVDD+S SR+N  H   C +   
Sbjct: 722 SSKL--CSTKHGRCDGSNTRPSHHHHHGHHHHSHRCHVVDDKSPSRDN--HNRDCGD--- 781

Query: 781 VPQQKENKFHHNPSNQCEKNQVEKESNGDSSRKVAESNCNCHS-HHVAIDIHEDTDCERV 839
                  KFHHN SNQCEKN  EKE+NG+ S KV E NCNCHS HHVAIDIHE TDCERV
Sbjct: 782 -------KFHHNDSNQCEKNPHEKENNGNCSTKVDEPNCNCHSHHHVAIDIHEGTDCERV 829

BLAST of Lcy08g012590 vs. ExPASy TrEMBL
Match: A0A6J1EPB0 (cadmium/zinc-transporting ATPase HMA2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436148 PE=3 SV=1)

HSP 1 Score: 1317.0 bits (3407), Expect = 0.0e+00
Identity = 699/840 (83.21%), Postives = 753/840 (89.64%), Query Frame = 0

Query: 1   MGEEAMEK-EKVMRENKLQRSHFDVLGLCCSSEVPLIENILKPLEGIKDISVIVPTRTLI 60
           +GE AMEK +KV RENK +RSHFDVLG+CCSSEVPLIENILKPLEGIK ISVIVPTRTLI
Sbjct: 2   VGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLI 61

Query: 61  VVHDSLLISQLQIVKALNEARLEANVQLNGKGISKKKWPSPFAIASGLLLTASFLKYVYH 120
           VVHDSLL+SQLQIVKALNEARLEANVQL GKGISKKKWPSPFAIASGLLLTASFLKYVYH
Sbjct: 62  VVHDSLLVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVYH 121

Query: 121 PLQWLAVAAVAAGIFPILLKAVSAVRHLRIDVNILAIIAVVGTIAMNDYMEAGSIVFLFS 180
           PL+WLAVAAVAAGIFPILLKA+SAVRHLRIDVNILAIIAVVG ++MNDYMEAGSIVFLFS
Sbjct: 122 PLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLFS 181

Query: 181 IAEWLESRASHKANAAMSSLMSLAPQTATIAETGEIVDSKEVKLNTVLAVKAGEVIPIDG 240
           IAEWLESRASHKANAAM SLMSLAPQ ATIAETGE+V+ KEV L +VLAVK GEVIPIDG
Sbjct: 182 IAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPIDG 241

Query: 241 IVVEGKCEVDEKTLTGESFPVAKQKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAELV 300
           IVV+GKC+VDEK+LTGE+FPV K KDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAE V
Sbjct: 242 IVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFV 301

Query: 301 EEAQNNKSKTQRFIDECAKYYTPAVLVISACLAAIPAALRVHNLSHWFHLALVVLVSACP 360
           EEAQNNKSKTQRFIDECAKYYTPAV+VISAC+AAIPAA RVHNLSHWFHLALVVLVSACP
Sbjct: 302 EEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACP 361

Query: 361 CALILSTPVAAFCALTKAAMAGVLIKGGDHLELLAKIKVMAFDKTGTITRGEFVVTDFRA 420
           CALILSTPVAAFCALTKAAMAGVLIKG DHLE+LAKIKV+AFDKTGTITRGEFVVTDF+A
Sbjct: 362 CALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTDFQA 421

Query: 421 LCDNISLHTLLHWVSSIESKSSHPMAAALVNHGKFLSVDIKPENVEEFENFPGEGVRGKI 480
           L D+IS HTLLHWVSSIESKSSHPMAAALVNH K LS DIKPE VEEFENFPGEGVRGKI
Sbjct: 422 LRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSTDIKPEKVEEFENFPGEGVRGKI 481

Query: 481 DGNNIYIGSRKIAARAGCPTAPSSDDDETKQGQTVGYVFCGTTLAGSFGLSDACRSGVRE 540
           DG +IYIGS KIAARAGC +A    ++E KQGQT+GYVFC     GSFGLSD+CRSG +E
Sbjct: 482 DGKDIYIGSTKIAARAGCSSAMKL-EEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKE 541

Query: 541 AIQEIKSFGIKTAMLTGDCNAAAMHAQQQLGNALDVIHSELLPKEKANIIKRFKGD-GPT 600
            ++E+KS GIKTAMLTGDC+AAAMHAQQQL NA+DVIHSELLPKEKAN+IK FK D G T
Sbjct: 542 GMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGAT 601

Query: 601 AMVGDGLNDTPALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHAKV 660
           AMVGDGLNDTPALATADIGISMG+SGS LAT+TGNVILMSND+ KIP+AIKLA+KSHAKV
Sbjct: 602 AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 661

Query: 661 VQNVILSIGTKTAILGLAFAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHRHGKKCCK 720
           VQNVILSI TK AILGLA AGHPLVWAAVLADVGTCLLVILNSMLLLRG+DH+HG KCCK
Sbjct: 662 VQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 721

Query: 721 SSKLPCFSTKHGKCNSSNGRSS--HRHDHHHDDHRCHVVDDRSSSRENRVHKHCCSEKNN 780
           SSKL   STKHG+C+ SN R S  H HDHHH DHRCHVVDD+  SR+N  H   C +   
Sbjct: 722 SSKL--CSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVVDDKWPSRDN--HNGDCGD--- 781

Query: 781 VPQQKENKFHHNPSNQCEKNQVEKESNGDSSRKVAESNCNCHSHHVAIDIHEDTDCERVE 837
                  KFHHN SNQCEK  +EK++NG+ S+KV E NCNCHSHHVAIDIH+  DCER+E
Sbjct: 782 -------KFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 826

BLAST of Lcy08g012590 vs. ExPASy TrEMBL
Match: A0A6J1K632 (putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492014 PE=3 SV=1)

HSP 1 Score: 1316.6 bits (3406), Expect = 0.0e+00
Identity = 699/848 (82.43%), Postives = 756/848 (89.15%), Query Frame = 0

Query: 1   MGEEAMEKEKVMR-ENKLQRSHFDVLGLCCSSEVPLIENILKPLEGIKDISVIVPTRTLI 60
           +GE AMEK K +R ENK +RSHFDVLG+CCSSEVPLIENILKPLEGIK ISVIVPTRTLI
Sbjct: 2   VGEVAMEKNKKVRGENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLI 61

Query: 61  VVHDSLLISQLQIVKALNEARLEANVQLNGKGISKKKWPSPFAIASGLLLTASFLKYVYH 120
           VVHDSLL+SQLQIVKALNEARLEANVQ+NGKGISKKKWPSPFAIASGLLLTASFLKYVYH
Sbjct: 62  VVHDSLLVSQLQIVKALNEARLEANVQVNGKGISKKKWPSPFAIASGLLLTASFLKYVYH 121

Query: 121 PLQWLAVAAVAAGIFPILLKAVSAVRHLRIDVNILAIIAVVGTIAMNDYMEAGSIVFLFS 180
           PL+WLA+AAVAAGIFPILLKA+SAVRHLRID NILAI+AVVGTI+MNDYMEAGSIVFLFS
Sbjct: 122 PLRWLAIAAVAAGIFPILLKAISAVRHLRIDANILAIVAVVGTISMNDYMEAGSIVFLFS 181

Query: 181 IAEWLESRASHKANAAMSSLMSLAPQTATIAETGEIVDSKEVKLNTVLAVKAGEVIPIDG 240
           IAEWLESRASHKANAAMSSLMSLAPQ A IAETGE+V  KEV+L +VLAVK GEVIPIDG
Sbjct: 182 IAEWLESRASHKANAAMSSLMSLAPQKAIIAETGEVVAVKEVELKSVLAVKGGEVIPIDG 241

Query: 241 IVVEGKCEVDEKTLTGESFPVAKQKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAELV 300
           IVV+GKC+VDEKTLTGE+FPV K KDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAE V
Sbjct: 242 IVVDGKCDVDEKTLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFV 301

Query: 301 EEAQNNKSKTQRFIDECAKYYTPAVLVISACLAAIPAALRVHNLSHWFHLALVVLVSACP 360
           EEAQNNKSKTQRFIDECAKYYTPAV+VISAC+AAIPAA RVHNLSHWFHLALVVLVSACP
Sbjct: 302 EEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACP 361

Query: 361 CALILSTPVAAFCALTKAAMAGVLIKGGDHLELLAKIKVMAFDKTGTITRGEFVVTDFRA 420
           CALILSTPVAAFCALTKAAMAGVLIKG DHLE+LAKIKV+AFDKTGTITRGEFVVT+F+A
Sbjct: 362 CALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTNFQA 421

Query: 421 LCDNISLHTLLHWVSSIESKSSHPMAAALVNHGKFLSVDIKPENVEEFENFPGEGVRGKI 480
           L D+IS HTLLHWVSSIESKSSHPMAAALVNH K LS+DIKPE VEEFENFPGEGVRGKI
Sbjct: 422 LRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGKI 481

Query: 481 DGNNIYIGSRKIAARAGCPTAPSSDDDETKQGQTVGYVFCGTTLAGSFGLSDACRSGVRE 540
           DG +IYIGS KIAARAGC +A    D+E KQGQT+GYVFCG    G+FGLSD+CRSG +E
Sbjct: 482 DGKDIYIGSTKIAARAGCSSAMKL-DEEMKQGQTLGYVFCGEMAVGAFGLSDSCRSGAKE 541

Query: 541 AIQEIKSFGIKTAMLTGDCNAAAMHAQQQLGNALDVIHSELLPKEKANIIKRFKGD-GPT 600
           A++++KS GIKTAMLTGDC+AAAMHAQQQL NA+DVIHSELLPKEKAN+IK FK D G T
Sbjct: 542 AMEQLKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANMIKEFKRDYGAT 601

Query: 601 AMVGDGLNDTPALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHAKV 660
           AMVGDGLNDTPALA ADIGISMG+SGS LAT+TGNVILMSND+ KIP+AIKLA+KSHAKV
Sbjct: 602 AMVGDGLNDTPALAIADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 661

Query: 661 VQNVILSIGTKTAILGLAFAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHRHGKKCCK 720
           VQNVILSI TK AILGLA AGHPLVWAAVLADVGTCLLVILNSMLLLRG+DH+HG KCCK
Sbjct: 662 VQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 721

Query: 721 SSKLPCFSTKHGKCNSSNGRSS---HRHDHHHDDHRCHVVDDRSSSRENRVHKHCCSEKN 780
           SSKL   S KHG+C+ SN R S   H HDHHH  HRCHVVDD+S SREN VHKHCC +K+
Sbjct: 722 SSKL--CSMKHGRCDGSNTRPSHHHHHHDHHHHSHRCHVVDDKSPSRENHVHKHCCEKKD 781

Query: 781 N---VPQQKE----NKFHHNPSNQCEKNQVEKESNGDSSRKVAESNCNCHSHHVAIDIHE 837
           +   +PQ       +KFHHN SN           NG+ S+KV E NCNCHSHHVAIDIHE
Sbjct: 782 HNLQLPQDHNGDCGDKFHHNHSN-----------NGNCSKKVGEPNCNCHSHHVAIDIHE 835

BLAST of Lcy08g012590 vs. ExPASy TrEMBL
Match: A0A6J1DIS0 (putative inactive cadmium/zinc-transporting ATPase HMA3 OS=Momordica charantia OX=3673 GN=LOC111020851 PE=3 SV=1)

HSP 1 Score: 1308.5 bits (3385), Expect = 0.0e+00
Identity = 706/841 (83.95%), Postives = 760/841 (90.37%), Query Frame = 0

Query: 6   MEKEKVMRENKLQRSHFDVLGLCCSSEVPLIENILKPLEGIKDISVIVPTRTLIVVHDSL 65
           ME EK   ENK Q+SHF+VLGLCCSSEVPLIENILKPLEGIK ISVIV TRT+IV HD L
Sbjct: 1   MEMEKKKGENKFQKSHFEVLGLCCSSEVPLIENILKPLEGIKQISVIVTTRTIIVHHDPL 60

Query: 66  LISQLQIVKALNEARLEANVQLNGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLQWLA 125
           LISQ+QIVKALNEARLEANV+L+GKGIS+KKWPSPFAIASGLLLTASFLKYVYHPL+WLA
Sbjct: 61  LISQIQIVKALNEARLEANVKLHGKGISQKKWPSPFAIASGLLLTASFLKYVYHPLRWLA 120

Query: 126 VAAVAAGIFPILLKAVSAVRHLRIDVNILAIIAVVGTIAMNDYMEAGSIVFLFSIAEWLE 185
           + AVAAGI PILLKA++AVRHLRIDVNILAIIAVVGTIAMNDYMEAGSIVFLFSIAEWLE
Sbjct: 121 IGAVAAGISPILLKAIAAVRHLRIDVNILAIIAVVGTIAMNDYMEAGSIVFLFSIAEWLE 180

Query: 186 SRASHKANAAMSSLMSLAPQTATIAETGEIVDSKEVKLNTVLAVKAGEVIPIDGIVVEGK 245
           SRAS KA++AM SLMSLAP+ A IAE+GE+VD+KEVKL +VLAVKAGEVIPIDGIVVEG 
Sbjct: 181 SRASQKASSAMCSLMSLAPRKAVIAESGEVVDAKEVKLKSVLAVKAGEVIPIDGIVVEGN 240

Query: 246 CEVDEKTLTGESFPVAKQKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAELVEEAQNN 305
           CEVDEKTLTGE+FPV K K SLVWAGTINLNGYISV+T+VVAEDCVVAKMAELVEEAQN+
Sbjct: 241 CEVDEKTLTGETFPVPKHKHSLVWAGTINLNGYISVETSVVAEDCVVAKMAELVEEAQNS 300

Query: 306 KSKTQRFIDECAKYYTPAVLVISACLAAIPAALRVHNLSHWFHLALVVLVSACPCALILS 365
           KSKTQRFIDECAKYYTPAV+VISAC+AAIPAALRVHNLSHW HLALVVLVSACPCALILS
Sbjct: 301 KSKTQRFIDECAKYYTPAVVVISACVAAIPAALRVHNLSHWLHLALVVLVSACPCALILS 360

Query: 366 TPVAAFCALTKAAMAGVLIKGGDHLELLAKIKVMAFDKTGTITRGEFVVTDFRALCDNIS 425
           TPVAAFCALTKAA AGVLIKGGDHLE+LAKIKVMAFDKTGTITRGEFVVTDFRAL D+IS
Sbjct: 361 TPVAAFCALTKAATAGVLIKGGDHLEILAKIKVMAFDKTGTITRGEFVVTDFRALRDDIS 420

Query: 426 LHTLLHWVSSIESKSSHPMAAALVNHGKFLSVDIKPENVEEFENFPGEGVRGKIDGNNIY 485
           LHTLLHWVS IESKSSHPMAAALVNHG+ LSV I PENVEEFENFPGEGVRGKIDGN+IY
Sbjct: 421 LHTLLHWVSCIESKSSHPMAAALVNHGRLLSVHINPENVEEFENFPGEGVRGKIDGNDIY 480

Query: 486 IGSRKIAARAGCPTAPSSDDDETKQGQTVGYVFCGTTLAGSFGLSDACRSGVREAIQEIK 545
           IGSRKIAARAGC T PS  DDETKQG TVGY+F G T+AGSF LSDACRSGVREAI+EIK
Sbjct: 481 IGSRKIAARAGCTTVPSL-DDETKQGLTVGYIFRGATIAGSFSLSDACRSGVREAIKEIK 540

Query: 546 SFGIKTAMLTGDCNAAAMHAQQQLGNALDVIHSELLPKEKANIIKRFKGDGPTAMVGDGL 605
           SFGIKTAMLTGDC+AAA HAQ+QLG+ LDVIHSELLPKEKANIIK  K DGPTAMVGDGL
Sbjct: 541 SFGIKTAMLTGDCSAAAAHAQEQLGDVLDVIHSELLPKEKANIIKELKRDGPTAMVGDGL 600

Query: 606 NDTPALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHAKVVQNVILS 665
           NDTPALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHAKVVQNVILS
Sbjct: 601 NDTPALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHAKVVQNVILS 660

Query: 666 IGTKTAILGLAFAGHPLVWAAVLADVGTCLLVILNSMLLLRGTD-HRHGKKCCKSSKLPC 725
           IGTKTAIL LAFAGHPLVWAAVLADVGTC++VILNSMLLLRG D H +  KCCKS+K PC
Sbjct: 661 IGTKTAILALAFAGHPLVWAAVLADVGTCVVVILNSMLLLRGADRHTNVNKCCKSAK-PC 720

Query: 726 FSTKHGKCNSSNGRSSHRHDH-HHDDHRCHVVDDRSSSR-ENRVHKHCCSEKNNVPQQKE 785
            STKH       GRSSH HDH HH +HRCHVVDD+S+ R EN VHKHCCSEK      K 
Sbjct: 721 -STKH-------GRSSHHHDHRHHHNHRCHVVDDKSTCRLENHVHKHCCSEK-----IKA 780

Query: 786 NKFHHNP-SNQCEKNQVEKE----SNGDSSRKVAESNCNCHSHHVAIDIHEDTDCERVEH 839
           +K H +P  N+ + +Q  +E    SNG+SSRKV+ESNCNCHSHH++IDIHE ++CER+EH
Sbjct: 781 HKLHLDPHENRIQGDQSCEEEHNGSNGNSSRKVSESNCNCHSHHISIDIHEGSNCERIEH 826

BLAST of Lcy08g012590 vs. ExPASy TrEMBL
Match: A0A6J1ENN0 (putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111436150 PE=3 SV=1)

HSP 1 Score: 1308.5 bits (3385), Expect = 0.0e+00
Identity = 694/839 (82.72%), Postives = 749/839 (89.27%), Query Frame = 0

Query: 1   MGEEAMEKEKVMRENKLQRSHFDVLGLCCSSEVPLIENILKPLEGIKDISVIVPTRTLIV 60
           +GE AMEK K   +NK +RSHFDVLG+CCSSEVPLIENILKPLEGIK ISVIVPTRTLIV
Sbjct: 2   VGEVAMEKNK---KNKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLIV 61

Query: 61  VHDSLLISQLQIVKALNEARLEANVQLNGKGISKKKWPSPFAIASGLLLTASFLKYVYHP 120
           VHDSLL+SQLQIVKALNEARLEA VQL GKGISKKKWPSPFAIASGLLLTASFLKYVYHP
Sbjct: 62  VHDSLLVSQLQIVKALNEARLEAKVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVYHP 121

Query: 121 LQWLAVAAVAAGIFPILLKAVSAVRHLRIDVNILAIIAVVGTIAMNDYMEAGSIVFLFSI 180
           L+WLAVAAVAAGIFPILLKA+SAVRHLRIDVNILAIIAVVGT++MNDYMEAGSIVFLFSI
Sbjct: 122 LRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGTMSMNDYMEAGSIVFLFSI 181

Query: 181 AEWLESRASHKANAAMSSLMSLAPQTATIAETGEIVDSKEVKLNTVLAVKAGEVIPIDGI 240
           AEWLESRASHKANAAM SLMSLAPQ ATIAETGE+V+ KEV L +VLAVK GEVIPIDGI
Sbjct: 182 AEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPIDGI 241

Query: 241 VVEGKCEVDEKTLTGESFPVAKQKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAELVE 300
           VV+GKC+VDEK+LTGE+FPV K KDSLVWAGTINLNGYISVQTT VAEDCVVAKMAE VE
Sbjct: 242 VVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTAVAEDCVVAKMAEFVE 301

Query: 301 EAQNNKSKTQRFIDECAKYYTPAVLVISACLAAIPAALRVHNLSHWFHLALVVLVSACPC 360
           EAQNNKSKTQRFIDECAKYYTPAV+VISAC+AAIPAA RVHNLSHWFHLALVVLVSACPC
Sbjct: 302 EAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACPC 361

Query: 361 ALILSTPVAAFCALTKAAMAGVLIKGGDHLELLAKIKVMAFDKTGTITRGEFVVTDFRAL 420
           ALILSTPVAAFCALTKAAMAGVLIKG DHLE+LAKIKV+AFDKTGTITRGEFVVTDF+AL
Sbjct: 362 ALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTDFQAL 421

Query: 421 CDNISLHTLLHWVSSIESKSSHPMAAALVNHGKFLSVDIKPENVEEFENFPGEGVRGKID 480
            D+IS HTLLHWVSSIESKSSHPMAAALVNH K LS DIKPE VEEFENFPGEGVRGKID
Sbjct: 422 RDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSTDIKPEKVEEFENFPGEGVRGKID 481

Query: 481 GNNIYIGSRKIAARAGCPTAPSSDDDETKQGQTVGYVFCGTTLAGSFGLSDACRSGVREA 540
           G +IYIGS KIAARAGC +A    ++E K GQT+GYVFC     GSFGLSD+CRSG +E 
Sbjct: 482 GKDIYIGSTKIAARAGCSSAMKL-EEEMKPGQTLGYVFCEEMAVGSFGLSDSCRSGAKEG 541

Query: 541 IQEIKSFGIKTAMLTGDCNAAAMHAQQQLGNALDVIHSELLPKEKANIIKRFKGD-GPTA 600
           ++E+KS GIKTAMLTGDC+AAAMHAQQQL NA+DVIHSELLPKEKAN+IK FK D G TA
Sbjct: 542 MEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANMIKEFKRDYGATA 601

Query: 601 MVGDGLNDTPALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHAKVV 660
           MVGDGLNDTPALATADIGISMG+SGS LAT+TGNVILMSND+ KIP+AIKLA+KSHAKVV
Sbjct: 602 MVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKVV 661

Query: 661 QNVILSIGTKTAILGLAFAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHRHGKKCCKS 720
           QNVILSI TK AILGLA AGHPLVWAAVLADVGTCLLVILNSMLLLRG+DH+HG KCCKS
Sbjct: 662 QNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCKS 721

Query: 721 SKLPCFSTKHGKCNSSNGRSS--HRHDHHHDDHRCHVVDDRSSSRENRVHKHCCSEKNNV 780
           SKL   STKHG+C+ SN R S  H HDHHH DHRCHV+DD+S SR+N  H   C +    
Sbjct: 722 SKL--CSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVIDDKSPSRDN--HNGDCGD---- 781

Query: 781 PQQKENKFHHNPSNQCEKNQVEKESNGDSSRKVAESNCNCHSHHVAIDIHEDTDCERVE 837
                 KFHHN SNQCEK  +EK++NG+ S+KV E NCNCHSHHVAIDIH+  DCER+E
Sbjct: 782 ------KFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 822

BLAST of Lcy08g012590 vs. NCBI nr
Match: XP_022997372.1 (putative inactive cadmium/zinc-transporting ATPase HMA3 [Cucurbita maxima])

HSP 1 Score: 1320.1 bits (3415), Expect = 0.0e+00
Identity = 702/843 (83.27%), Postives = 757/843 (89.80%), Query Frame = 0

Query: 1   MGEEAMEK-EKVMRENKLQRSHFDVLGLCCSSEVPLIENILKPLEGIKDISVIVPTRTLI 60
           +GE AMEK +KV RENK +RSHFDVLG+CCSSEVPL+ENILKPLEGIK ISVIVPTRTLI
Sbjct: 2   VGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLVENILKPLEGIKQISVIVPTRTLI 61

Query: 61  VVHDSLLISQLQIVKALNEARLEANVQLNGKGISKKKWPSPFAIASGLLLTASFLKYVYH 120
           VVHDSLL+SQLQIVKALNEARLEANVQ+NGKGISKKKWPSPFAIASGLLLTASFLKYVYH
Sbjct: 62  VVHDSLLVSQLQIVKALNEARLEANVQVNGKGISKKKWPSPFAIASGLLLTASFLKYVYH 121

Query: 121 PLQWLAVAAVAAGIFPILLKAVSAVRHLRIDVNILAIIAVVGTIAMNDYMEAGSIVFLFS 180
           PL+WLA+AAVAAGIFPILLKA+SAVRHLRID NILAI+AVVGTI+MNDYMEAGSIVFLFS
Sbjct: 122 PLRWLAIAAVAAGIFPILLKAISAVRHLRIDANILAIVAVVGTISMNDYMEAGSIVFLFS 181

Query: 181 IAEWLESRASHKANAAMSSLMSLAPQTATIAETGEIVDSKEVKLNTVLAVKAGEVIPIDG 240
           IAEWLESRASHKANAAMSSLMSLAPQ A IAETGE+V  KEV+L +VLAVK GEVIPIDG
Sbjct: 182 IAEWLESRASHKANAAMSSLMSLAPQKAIIAETGEVVAVKEVELKSVLAVKGGEVIPIDG 241

Query: 241 IVVEGKCEVDEKTLTGESFPVAKQKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAELV 300
           IVV+GKC+VDEKTLTGE+FPV K KDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAE V
Sbjct: 242 IVVDGKCDVDEKTLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFV 301

Query: 301 EEAQNNKSKTQRFIDECAKYYTPAVLVISACLAAIPAALRVHNLSHWFHLALVVLVSACP 360
           EEAQNNKSKTQRFIDECAKYYTPAV+VISAC+AAIPAA RVHNLSHWFHLALVVLVSACP
Sbjct: 302 EEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACP 361

Query: 361 CALILSTPVAAFCALTKAAMAGVLIKGGDHLELLAKIKVMAFDKTGTITRGEFVVTDFRA 420
           CALILSTPVAAFCALTKAAMAGVLIKG DHLE+LAKIKV+AFDKTGTITRGEFVVT+F+A
Sbjct: 362 CALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTNFQA 421

Query: 421 LCDNISLHTLLHWVSSIESKSSHPMAAALVNHGKFLSVDIKPENVEEFENFPGEGVRGKI 480
           L D+I+ HTLLHWVSSIESKSSHPMAAALVNH K LS+DIKPE VEEFENFPGEGVRGKI
Sbjct: 422 LRDDINFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGKI 481

Query: 481 DGNNIYIGSRKIAARAGCPTAPSSDDDETKQGQTVGYVFCGTTLAGSFGLSDACRSGVRE 540
           DG +IYIGS KIAARAGC +A    D+E KQGQT+GYVFCG    G+FGLSD+CRSG +E
Sbjct: 482 DGKDIYIGSTKIAARAGCSSAMKL-DEEMKQGQTLGYVFCGEMAVGAFGLSDSCRSGAKE 541

Query: 541 AIQEIKSFGIKTAMLTGDCNAAAMHAQQQLGNALDVIHSELLPKEKANIIKRFKGD-GPT 600
           A++++KS GIKTAMLTGDC+AAAMHAQQQL NA+DVIHSELLPKEKAN+IK FK D G T
Sbjct: 542 AMEQLKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANMIKEFKRDYGAT 601

Query: 601 AMVGDGLNDTPALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHAKV 660
           AMVGDGLNDTPALATADIGISMG+SGS LAT+TGNVILMSND+ KIP+AIKLA+KSHAKV
Sbjct: 602 AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 661

Query: 661 VQNVILSIGTKTAILGLAFAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHRHGKKCCK 720
           VQNVILSI TK AILGLA AGHPLVWAAVLADVGTCLLVILNSMLLLRG+DH+HG KCCK
Sbjct: 662 VQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 721

Query: 721 SSKLPCFSTKHGKCNSSNGRSS--HRHDHHHDDHRCHVVDDRSSSRENRVHKHCCSEKNN 780
           SSKL   STKHG+C+ SN R S  H H HHH  HRCHVVDD+S SR+N  H   C +   
Sbjct: 722 SSKL--CSTKHGRCDGSNTRPSHHHHHGHHHHSHRCHVVDDKSPSRDN--HNRDCGD--- 781

Query: 781 VPQQKENKFHHNPSNQCEKNQVEKESNGDSSRKVAESNCNCHS-HHVAIDIHEDTDCERV 839
                  KFHHN SNQCEKN  EKE+NG+ S KV E NCNCHS HHVAIDIHE TDCERV
Sbjct: 782 -------KFHHNDSNQCEKNPHEKENNGNCSTKVDEPNCNCHSHHHVAIDIHEGTDCERV 829

BLAST of Lcy08g012590 vs. NCBI nr
Match: XP_022929619.1 (cadmium/zinc-transporting ATPase HMA2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1317.0 bits (3407), Expect = 0.0e+00
Identity = 699/840 (83.21%), Postives = 753/840 (89.64%), Query Frame = 0

Query: 1   MGEEAMEK-EKVMRENKLQRSHFDVLGLCCSSEVPLIENILKPLEGIKDISVIVPTRTLI 60
           +GE AMEK +KV RENK +RSHFDVLG+CCSSEVPLIENILKPLEGIK ISVIVPTRTLI
Sbjct: 2   VGEVAMEKNKKVRRENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLI 61

Query: 61  VVHDSLLISQLQIVKALNEARLEANVQLNGKGISKKKWPSPFAIASGLLLTASFLKYVYH 120
           VVHDSLL+SQLQIVKALNEARLEANVQL GKGISKKKWPSPFAIASGLLLTASFLKYVYH
Sbjct: 62  VVHDSLLVSQLQIVKALNEARLEANVQLKGKGISKKKWPSPFAIASGLLLTASFLKYVYH 121

Query: 121 PLQWLAVAAVAAGIFPILLKAVSAVRHLRIDVNILAIIAVVGTIAMNDYMEAGSIVFLFS 180
           PL+WLAVAAVAAGIFPILLKA+SAVRHLRIDVNILAIIAVVG ++MNDYMEAGSIVFLFS
Sbjct: 122 PLRWLAVAAVAAGIFPILLKAISAVRHLRIDVNILAIIAVVGAMSMNDYMEAGSIVFLFS 181

Query: 181 IAEWLESRASHKANAAMSSLMSLAPQTATIAETGEIVDSKEVKLNTVLAVKAGEVIPIDG 240
           IAEWLESRASHKANAAM SLMSLAPQ ATIAETGE+V+ KEV L +VLAVK GEVIPIDG
Sbjct: 182 IAEWLESRASHKANAAMWSLMSLAPQKATIAETGEVVEVKEVTLKSVLAVKGGEVIPIDG 241

Query: 241 IVVEGKCEVDEKTLTGESFPVAKQKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAELV 300
           IVV+GKC+VDEK+LTGE+FPV K KDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAE V
Sbjct: 242 IVVDGKCDVDEKSLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFV 301

Query: 301 EEAQNNKSKTQRFIDECAKYYTPAVLVISACLAAIPAALRVHNLSHWFHLALVVLVSACP 360
           EEAQNNKSKTQRFIDECAKYYTPAV+VISAC+AAIPAA RVHNLSHWFHLALVVLVSACP
Sbjct: 302 EEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACP 361

Query: 361 CALILSTPVAAFCALTKAAMAGVLIKGGDHLELLAKIKVMAFDKTGTITRGEFVVTDFRA 420
           CALILSTPVAAFCALTKAAMAGVLIKG DHLE+LAKIKV+AFDKTGTITRGEFVVTDF+A
Sbjct: 362 CALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTDFQA 421

Query: 421 LCDNISLHTLLHWVSSIESKSSHPMAAALVNHGKFLSVDIKPENVEEFENFPGEGVRGKI 480
           L D+IS HTLLHWVSSIESKSSHPMAAALVNH K LS DIKPE VEEFENFPGEGVRGKI
Sbjct: 422 LRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSTDIKPEKVEEFENFPGEGVRGKI 481

Query: 481 DGNNIYIGSRKIAARAGCPTAPSSDDDETKQGQTVGYVFCGTTLAGSFGLSDACRSGVRE 540
           DG +IYIGS KIAARAGC +A    ++E KQGQT+GYVFC     GSFGLSD+CRSG +E
Sbjct: 482 DGKDIYIGSTKIAARAGCSSAMKL-EEEMKQGQTLGYVFCEEMAVGSFGLSDSCRSGAKE 541

Query: 541 AIQEIKSFGIKTAMLTGDCNAAAMHAQQQLGNALDVIHSELLPKEKANIIKRFKGD-GPT 600
            ++E+KS GIKTAMLTGDC+AAAMHAQQQL NA+DVIHSELLPKEKAN+IK FK D G T
Sbjct: 542 GMEELKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANVIKEFKRDYGAT 601

Query: 601 AMVGDGLNDTPALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHAKV 660
           AMVGDGLNDTPALATADIGISMG+SGS LAT+TGNVILMSND+ KIP+AIKLA+KSHAKV
Sbjct: 602 AMVGDGLNDTPALATADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 661

Query: 661 VQNVILSIGTKTAILGLAFAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHRHGKKCCK 720
           VQNVILSI TK AILGLA AGHPLVWAAVLADVGTCLLVILNSMLLLRG+DH+HG KCCK
Sbjct: 662 VQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 721

Query: 721 SSKLPCFSTKHGKCNSSNGRSS--HRHDHHHDDHRCHVVDDRSSSRENRVHKHCCSEKNN 780
           SSKL   STKHG+C+ SN R S  H HDHHH DHRCHVVDD+  SR+N  H   C +   
Sbjct: 722 SSKL--CSTKHGRCDGSNTRPSHHHHHDHHHHDHRCHVVDDKWPSRDN--HNGDCGD--- 781

Query: 781 VPQQKENKFHHNPSNQCEKNQVEKESNGDSSRKVAESNCNCHSHHVAIDIHEDTDCERVE 837
                  KFHHN SNQCEK  +EK++NG+ S+KV E NCNCHSHHVAIDIH+  DCER+E
Sbjct: 782 -------KFHHNRSNQCEKTPLEKDNNGNCSKKVGEPNCNCHSHHVAIDIHQGIDCERIE 826

BLAST of Lcy08g012590 vs. NCBI nr
Match: XP_038891185.1 (LOW QUALITY PROTEIN: putative inactive cadmium/zinc-transporting ATPase HMA3 [Benincasa hispida])

HSP 1 Score: 1316.6 bits (3406), Expect = 0.0e+00
Identity = 712/868 (82.03%), Postives = 765/868 (88.13%), Query Frame = 0

Query: 3   EEAMEK--EKVMRENKLQRSHFDVLGLCCSSEVPLIENILKPLEGIKDISVIVPTRTLIV 62
           +EA+EK  +KV+RENKLQRS+FDVLG+CCSSEVPLIENILKPLEGIK ISVIVPTRTLIV
Sbjct: 4   QEALEKKNKKVVRENKLQRSYFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLIV 63

Query: 63  VHDSLLISQLQIVKALNEARLEANVQLNGKGISKKKWPSPFAIASGLLLTASFLKYVYHP 122
           VHDSLLISQLQIVKALNEARLEANVQLNGKGISKK+WPSPFAIASGLLLTASFLKYVYHP
Sbjct: 64  VHDSLLISQLQIVKALNEARLEANVQLNGKGISKKRWPSPFAIASGLLLTASFLKYVYHP 123

Query: 123 LQWLAVAAVAAGIFPILLKAVSAVRHLRIDVNILAIIAVVGTIAMNDYMEAGSIVFLFSI 182
           L+WLAVAAVAAGIFPILLKA+SA+RHLRIDVNILAI+AVVGTIAM+DYMEAGSIVFLFSI
Sbjct: 124 LRWLAVAAVAAGIFPILLKAISAIRHLRIDVNILAIVAVVGTIAMDDYMEAGSIVFLFSI 183

Query: 183 AEWLESRASHKANAAMSSLMSLAPQTATIAETGEIVDSKEVKLNTVLAVKAGEVIPIDGI 242
           AEWLESRASHKANAA  +LMSLAPQ     +  +IVD+KEVKLN+VLAVKAG VIPIDGI
Sbjct: 184 AEWLESRASHKANAAXCTLMSLAPQKTLXLKLEKIVDAKEVKLNSVLAVKAGRVIPIDGI 243

Query: 243 VVEGKCEVDEKTLTGESFPVAKQKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAELVE 302
           V+EGK EVDEKTLTG +FP +K KDSLVW GTINLNGYISV+TT VAEDCVVAKMAELVE
Sbjct: 244 VIEGKSEVDEKTLTGRNFPSSKHKDSLVWXGTINLNGYISVRTTAVAEDCVVAKMAELVE 303

Query: 303 EAQNNKSKTQRFIDECAKYYTPAVLVISACLAAIPAALRVHNLSHWFHLALVVLVSACPC 362
           EAQNNKSKTQRFIDECAKYYTPAV++ISACLAAIPAALRVHNLSHW HLALVVLVSACPC
Sbjct: 304 EAQNNKSKTQRFIDECAKYYTPAVVIISACLAAIPAALRVHNLSHWLHLALVVLVSACPC 363

Query: 363 ALILSTPVAAFCALTKAAMAGVLIKGGDHLELLAKIKVMAFDKTGTITRGEFVVTDFRAL 422
           ALILSTPVAAFCALTKAAMAGVLIKGGDHLE+LAK+KVMAFDKTGTITRGEFVVT F+AL
Sbjct: 364 ALILSTPVAAFCALTKAAMAGVLIKGGDHLEVLAKVKVMAFDKTGTITRGEFVVTHFQAL 423

Query: 423 CDNISLHTLLHWVSSIESKSSHPMAAALVNHGKFLSVDIKPENVEEFENFPGEGVRGKID 482
            D+IS HTLLHWVSSIESKSSHPMAAALVNHGK LS+D+KP+NVEEFENFPGEGVRGKID
Sbjct: 424 RDDISFHTLLHWVSSIESKSSHPMAAALVNHGKLLSIDLKPKNVEEFENFPGEGVRGKID 483

Query: 483 GNNIYIGSRKIAARAGCPTAPSSDDDETKQGQTVGYVFCGTTLAGSFGLSDACRSGVREA 542
           GN+IYIGSRKIA RAG  T   S DDETKQ QT+GYVFCG T+ GSFGLSD+CRSGV+EA
Sbjct: 484 GNDIYIGSRKIAVRAGY-TPDLSFDDETKQEQTLGYVFCGGTIIGSFGLSDSCRSGVKEA 543

Query: 543 IQEIKSFGIKTAMLTGDCNAAAMHAQQQLGNALDVIHSELLPKEKANIIKRFKGDGP-TA 602
           I+EIKSFGIKTAMLTGDCNAAAMHAQ+QLGNALD+IHSELLPKEKANIIK FK DG   A
Sbjct: 544 IEEIKSFGIKTAMLTGDCNAAAMHAQEQLGNALDIIHSELLPKEKANIIKDFKEDGAIIA 603

Query: 603 MVGDGLNDTPALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHAKVV 662
           MVGDGLNDTPAL TADIGISMGVSGSALATETGNVILMSNDMRKIPK IKLAKK H KVV
Sbjct: 604 MVGDGLNDTPALVTADIGISMGVSGSALATETGNVILMSNDMRKIPKTIKLAKKFHKKVV 663

Query: 663 QNVILSIGTKTAILGLAFAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHRHGKKCCKS 722
           QNVILSI TKTAILGLAFAGHPLVWAAVLADVGTCLLVILNSMLLLRG +H+HG+KCCKS
Sbjct: 664 QNVILSISTKTAILGLAFAGHPLVWAAVLADVGTCLLVILNSMLLLRGINHKHGEKCCKS 723

Query: 723 SKLPCFSTKHGKCNSSNGR-SSHRHD--HHHDDHRCHVVDDRSSSRE-NRVHKHC----- 782
           SK PC +    +C+  N R SSH HD  HHH  HRCHV DD+SSSRE N VHKH      
Sbjct: 724 SK-PCLTKYGQRCDGGNTRLSSHHHDHHHHHHHHRCHVADDQSSSRENNHVHKHYKIELP 783

Query: 783 -----------------CS---EKNNVPQQKENKFHHNPSNQCEKNQVEKESNGDSSRKV 839
                            CS   E+ NV  +KE+KF++N SNQC     EKESN +SS+KV
Sbjct: 784 QDHDRENSEVNQKKNHQCSDECEETNVAHEKEHKFYYNHSNQC-----EKESNENSSKKV 843

BLAST of Lcy08g012590 vs. NCBI nr
Match: XP_022996926.1 (putative inactive cadmium/zinc-transporting ATPase HMA3 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1316.6 bits (3406), Expect = 0.0e+00
Identity = 699/848 (82.43%), Postives = 756/848 (89.15%), Query Frame = 0

Query: 1   MGEEAMEKEKVMR-ENKLQRSHFDVLGLCCSSEVPLIENILKPLEGIKDISVIVPTRTLI 60
           +GE AMEK K +R ENK +RSHFDVLG+CCSSEVPLIENILKPLEGIK ISVIVPTRTLI
Sbjct: 2   VGEVAMEKNKKVRGENKFERSHFDVLGICCSSEVPLIENILKPLEGIKQISVIVPTRTLI 61

Query: 61  VVHDSLLISQLQIVKALNEARLEANVQLNGKGISKKKWPSPFAIASGLLLTASFLKYVYH 120
           VVHDSLL+SQLQIVKALNEARLEANVQ+NGKGISKKKWPSPFAIASGLLLTASFLKYVYH
Sbjct: 62  VVHDSLLVSQLQIVKALNEARLEANVQVNGKGISKKKWPSPFAIASGLLLTASFLKYVYH 121

Query: 121 PLQWLAVAAVAAGIFPILLKAVSAVRHLRIDVNILAIIAVVGTIAMNDYMEAGSIVFLFS 180
           PL+WLA+AAVAAGIFPILLKA+SAVRHLRID NILAI+AVVGTI+MNDYMEAGSIVFLFS
Sbjct: 122 PLRWLAIAAVAAGIFPILLKAISAVRHLRIDANILAIVAVVGTISMNDYMEAGSIVFLFS 181

Query: 181 IAEWLESRASHKANAAMSSLMSLAPQTATIAETGEIVDSKEVKLNTVLAVKAGEVIPIDG 240
           IAEWLESRASHKANAAMSSLMSLAPQ A IAETGE+V  KEV+L +VLAVK GEVIPIDG
Sbjct: 182 IAEWLESRASHKANAAMSSLMSLAPQKAIIAETGEVVAVKEVELKSVLAVKGGEVIPIDG 241

Query: 241 IVVEGKCEVDEKTLTGESFPVAKQKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAELV 300
           IVV+GKC+VDEKTLTGE+FPV K KDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAE V
Sbjct: 242 IVVDGKCDVDEKTLTGETFPVPKLKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAEFV 301

Query: 301 EEAQNNKSKTQRFIDECAKYYTPAVLVISACLAAIPAALRVHNLSHWFHLALVVLVSACP 360
           EEAQNNKSKTQRFIDECAKYYTPAV+VISAC+AAIPAA RVHNLSHWFHLALVVLVSACP
Sbjct: 302 EEAQNNKSKTQRFIDECAKYYTPAVVVISACVAAIPAAFRVHNLSHWFHLALVVLVSACP 361

Query: 361 CALILSTPVAAFCALTKAAMAGVLIKGGDHLELLAKIKVMAFDKTGTITRGEFVVTDFRA 420
           CALILSTPVAAFCALTKAAMAGVLIKG DHLE+LAKIKV+AFDKTGTITRGEFVVT+F+A
Sbjct: 362 CALILSTPVAAFCALTKAAMAGVLIKGSDHLEVLAKIKVVAFDKTGTITRGEFVVTNFQA 421

Query: 421 LCDNISLHTLLHWVSSIESKSSHPMAAALVNHGKFLSVDIKPENVEEFENFPGEGVRGKI 480
           L D+IS HTLLHWVSSIESKSSHPMAAALVNH K LS+DIKPE VEEFENFPGEGVRGKI
Sbjct: 422 LRDDISFHTLLHWVSSIESKSSHPMAAALVNHAKLLSIDIKPEKVEEFENFPGEGVRGKI 481

Query: 481 DGNNIYIGSRKIAARAGCPTAPSSDDDETKQGQTVGYVFCGTTLAGSFGLSDACRSGVRE 540
           DG +IYIGS KIAARAGC +A    D+E KQGQT+GYVFCG    G+FGLSD+CRSG +E
Sbjct: 482 DGKDIYIGSTKIAARAGCSSAMKL-DEEMKQGQTLGYVFCGEMAVGAFGLSDSCRSGAKE 541

Query: 541 AIQEIKSFGIKTAMLTGDCNAAAMHAQQQLGNALDVIHSELLPKEKANIIKRFKGD-GPT 600
           A++++KS GIKTAMLTGDC+AAAMHAQQQL NA+DVIHSELLPKEKAN+IK FK D G T
Sbjct: 542 AMEQLKSLGIKTAMLTGDCSAAAMHAQQQLENAVDVIHSELLPKEKANMIKEFKRDYGAT 601

Query: 601 AMVGDGLNDTPALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHAKV 660
           AMVGDGLNDTPALA ADIGISMG+SGS LAT+TGNVILMSND+ KIP+AIKLA+KSHAKV
Sbjct: 602 AMVGDGLNDTPALAIADIGISMGISGSPLATQTGNVILMSNDITKIPQAIKLARKSHAKV 661

Query: 661 VQNVILSIGTKTAILGLAFAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHRHGKKCCK 720
           VQNVILSI TK AILGLA AGHPLVWAAVLADVGTCLLVILNSMLLLRG+DH+HG KCCK
Sbjct: 662 VQNVILSITTKIAILGLAIAGHPLVWAAVLADVGTCLLVILNSMLLLRGSDHKHGNKCCK 721

Query: 721 SSKLPCFSTKHGKCNSSNGRSS---HRHDHHHDDHRCHVVDDRSSSRENRVHKHCCSEKN 780
           SSKL   S KHG+C+ SN R S   H HDHHH  HRCHVVDD+S SREN VHKHCC +K+
Sbjct: 722 SSKL--CSMKHGRCDGSNTRPSHHHHHHDHHHHSHRCHVVDDKSPSRENHVHKHCCEKKD 781

Query: 781 N---VPQQKE----NKFHHNPSNQCEKNQVEKESNGDSSRKVAESNCNCHSHHVAIDIHE 837
           +   +PQ       +KFHHN SN           NG+ S+KV E NCNCHSHHVAIDIHE
Sbjct: 782 HNLQLPQDHNGDCGDKFHHNHSN-----------NGNCSKKVGEPNCNCHSHHVAIDIHE 835

BLAST of Lcy08g012590 vs. NCBI nr
Match: XP_022153334.1 (putative inactive cadmium/zinc-transporting ATPase HMA3 [Momordica charantia])

HSP 1 Score: 1308.5 bits (3385), Expect = 0.0e+00
Identity = 706/841 (83.95%), Postives = 760/841 (90.37%), Query Frame = 0

Query: 6   MEKEKVMRENKLQRSHFDVLGLCCSSEVPLIENILKPLEGIKDISVIVPTRTLIVVHDSL 65
           ME EK   ENK Q+SHF+VLGLCCSSEVPLIENILKPLEGIK ISVIV TRT+IV HD L
Sbjct: 1   MEMEKKKGENKFQKSHFEVLGLCCSSEVPLIENILKPLEGIKQISVIVTTRTIIVHHDPL 60

Query: 66  LISQLQIVKALNEARLEANVQLNGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLQWLA 125
           LISQ+QIVKALNEARLEANV+L+GKGIS+KKWPSPFAIASGLLLTASFLKYVYHPL+WLA
Sbjct: 61  LISQIQIVKALNEARLEANVKLHGKGISQKKWPSPFAIASGLLLTASFLKYVYHPLRWLA 120

Query: 126 VAAVAAGIFPILLKAVSAVRHLRIDVNILAIIAVVGTIAMNDYMEAGSIVFLFSIAEWLE 185
           + AVAAGI PILLKA++AVRHLRIDVNILAIIAVVGTIAMNDYMEAGSIVFLFSIAEWLE
Sbjct: 121 IGAVAAGISPILLKAIAAVRHLRIDVNILAIIAVVGTIAMNDYMEAGSIVFLFSIAEWLE 180

Query: 186 SRASHKANAAMSSLMSLAPQTATIAETGEIVDSKEVKLNTVLAVKAGEVIPIDGIVVEGK 245
           SRAS KA++AM SLMSLAP+ A IAE+GE+VD+KEVKL +VLAVKAGEVIPIDGIVVEG 
Sbjct: 181 SRASQKASSAMCSLMSLAPRKAVIAESGEVVDAKEVKLKSVLAVKAGEVIPIDGIVVEGN 240

Query: 246 CEVDEKTLTGESFPVAKQKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAELVEEAQNN 305
           CEVDEKTLTGE+FPV K K SLVWAGTINLNGYISV+T+VVAEDCVVAKMAELVEEAQN+
Sbjct: 241 CEVDEKTLTGETFPVPKHKHSLVWAGTINLNGYISVETSVVAEDCVVAKMAELVEEAQNS 300

Query: 306 KSKTQRFIDECAKYYTPAVLVISACLAAIPAALRVHNLSHWFHLALVVLVSACPCALILS 365
           KSKTQRFIDECAKYYTPAV+VISAC+AAIPAALRVHNLSHW HLALVVLVSACPCALILS
Sbjct: 301 KSKTQRFIDECAKYYTPAVVVISACVAAIPAALRVHNLSHWLHLALVVLVSACPCALILS 360

Query: 366 TPVAAFCALTKAAMAGVLIKGGDHLELLAKIKVMAFDKTGTITRGEFVVTDFRALCDNIS 425
           TPVAAFCALTKAA AGVLIKGGDHLE+LAKIKVMAFDKTGTITRGEFVVTDFRAL D+IS
Sbjct: 361 TPVAAFCALTKAATAGVLIKGGDHLEILAKIKVMAFDKTGTITRGEFVVTDFRALRDDIS 420

Query: 426 LHTLLHWVSSIESKSSHPMAAALVNHGKFLSVDIKPENVEEFENFPGEGVRGKIDGNNIY 485
           LHTLLHWVS IESKSSHPMAAALVNHG+ LSV I PENVEEFENFPGEGVRGKIDGN+IY
Sbjct: 421 LHTLLHWVSCIESKSSHPMAAALVNHGRLLSVHINPENVEEFENFPGEGVRGKIDGNDIY 480

Query: 486 IGSRKIAARAGCPTAPSSDDDETKQGQTVGYVFCGTTLAGSFGLSDACRSGVREAIQEIK 545
           IGSRKIAARAGC T PS  DDETKQG TVGY+F G T+AGSF LSDACRSGVREAI+EIK
Sbjct: 481 IGSRKIAARAGCTTVPSL-DDETKQGLTVGYIFRGATIAGSFSLSDACRSGVREAIKEIK 540

Query: 546 SFGIKTAMLTGDCNAAAMHAQQQLGNALDVIHSELLPKEKANIIKRFKGDGPTAMVGDGL 605
           SFGIKTAMLTGDC+AAA HAQ+QLG+ LDVIHSELLPKEKANIIK  K DGPTAMVGDGL
Sbjct: 541 SFGIKTAMLTGDCSAAAAHAQEQLGDVLDVIHSELLPKEKANIIKELKRDGPTAMVGDGL 600

Query: 606 NDTPALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHAKVVQNVILS 665
           NDTPALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHAKVVQNVILS
Sbjct: 601 NDTPALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHAKVVQNVILS 660

Query: 666 IGTKTAILGLAFAGHPLVWAAVLADVGTCLLVILNSMLLLRGTD-HRHGKKCCKSSKLPC 725
           IGTKTAIL LAFAGHPLVWAAVLADVGTC++VILNSMLLLRG D H +  KCCKS+K PC
Sbjct: 661 IGTKTAILALAFAGHPLVWAAVLADVGTCVVVILNSMLLLRGADRHTNVNKCCKSAK-PC 720

Query: 726 FSTKHGKCNSSNGRSSHRHDH-HHDDHRCHVVDDRSSSR-ENRVHKHCCSEKNNVPQQKE 785
            STKH       GRSSH HDH HH +HRCHVVDD+S+ R EN VHKHCCSEK      K 
Sbjct: 721 -STKH-------GRSSHHHDHRHHHNHRCHVVDDKSTCRLENHVHKHCCSEK-----IKA 780

Query: 786 NKFHHNP-SNQCEKNQVEKE----SNGDSSRKVAESNCNCHSHHVAIDIHEDTDCERVEH 839
           +K H +P  N+ + +Q  +E    SNG+SSRKV+ESNCNCHSHH++IDIHE ++CER+EH
Sbjct: 781 HKLHLDPHENRIQGDQSCEEEHNGSNGNSSRKVSESNCNCHSHHISIDIHEGSNCERIEH 826

BLAST of Lcy08g012590 vs. TAIR 10
Match: AT2G19110.1 (heavy metal atpase 4 )

HSP 1 Score: 959.9 bits (2480), Expect = 1.3e-279
Identity = 513/836 (61.36%), Postives = 650/836 (77.75%), Query Frame = 0

Query: 7   EKEKVMRENKLQRSHFDVLGLCCSSEVPLIENILKPLEGIKDISVIVPTRTLIVVHDSLL 66
           EK+KV    KLQ+S+FDVLG+CC+SEVP+IENILK L+G+K+ SVIVP+RT+IVVHDSLL
Sbjct: 9   EKKKV---KKLQKSYFDVLGICCTSEVPIIENILKSLDGVKEYSVIVPSRTVIVVHDSLL 68

Query: 67  ISQLQIVKALNEARLEANVQLNGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLQWLAV 126
           IS  QI KALNEARLEANV++NG+   K KWPSPFA+ SGLLL  SFLK+VY PL+WLAV
Sbjct: 69  ISPFQIAKALNEARLEANVRVNGETSFKNKWPSPFAVVSGLLLLLSFLKFVYSPLRWLAV 128

Query: 127 AAVAAGIFPILLKAVSAVRHLRIDVNILAIIAVVGTIAMNDYMEAGSIVFLFSIAEWLES 186
           AAVAAGI+PIL KA ++++  RID+NIL II V+ T+AM D+MEA ++VFLF+I++WLE+
Sbjct: 129 AAVAAGIYPILAKAFASIKRPRIDINILVIITVIATLAMQDFMEAAAVVFLFTISDWLET 188

Query: 187 RASHKANAAMSSLMSLAPQTATIAETGEIVDSKEVKLNTVLAVKAGEVIPIDGIVVEGKC 246
           RAS+KA + M SLMSLAPQ A IAETGE V+  EVK++TV+AVKAGE IPIDGIVV+G C
Sbjct: 189 RASYKATSVMQSLMSLAPQKAIIAETGEEVEVDEVKVDTVVAVKAGETIPIDGIVVDGNC 248

Query: 247 EVDEKTLTGESFPVAKQKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAELVEEAQNNK 306
           EVDEKTLTGE+FPV KQ+DS VWAGTINLNGYI V+TT +A DCVVAKMA+LVEEAQ++K
Sbjct: 249 EVDEKTLTGEAFPVPKQRDSTVWAGTINLNGYICVKTTSLAGDCVVAKMAKLVEEAQSSK 308

Query: 307 SKTQRFIDECAKYYTPAVLVISACLAAIPAALRVHNLSHWFHLALVVLVSACPCALILST 366
           +K+QR ID+C++YYTPA++++SAC+A +P  ++VHNL HWFHLALVVLVS CPC LILST
Sbjct: 309 TKSQRLIDKCSQYYTPAIILVSACVAIVPVIMKVHNLKHWFHLALVVLVSGCPCGLILST 368

Query: 367 PVAAFCALTKAAMAGVLIKGGDHLELLAKIKVMAFDKTGTITRGEFVVTDFRALCDNISL 426
           PVA FCALTKAA +G+LIK  D+L+ L+KIK++AFDKTGTITRGEF+V DF++L  +I+L
Sbjct: 369 PVATFCALTKAATSGLLIKSADYLDTLSKIKIVAFDKTGTITRGEFIVIDFKSLSRDINL 428

Query: 427 HTLLHWVSSIESKSSHPMAAALVNHGKFLSVDIKPENVEEFENFPGEGVRGKIDGNNIYI 486
            +LL+WVSS+ESKSSHPMAA +V++ K +SV+ +PE VE+++NFPGEG+ GKIDGN+I+I
Sbjct: 429 RSLLYWVSSVESKSSHPMAATIVDYAKSVSVEPRPEEVEDYQNFPGEGIYGKIDGNDIFI 488

Query: 487 GSRKIAARAGCPTAPSSDDDETKQGQTVGYVFCGTTLAGSFGLSDACRSGVREAIQEIKS 546
           G++KIA+RAGC T P  + D TK G+TVGYV+ G  LAG F LSDACRSGV +A+ E+KS
Sbjct: 489 GNKKIASRAGCSTVPEIEVD-TKGGKTVGYVYVGERLAGFFNLSDACRSGVSQAMAELKS 548

Query: 547 FGIKTAMLTGDCNAAAMHAQQQLGNALDVIHSELLPKEKANIIKRFKGDGPTAMVGDGLN 606
            GIKTAMLTGD  AAAMHAQ+QLGN LDV+H +LLP++K+ II+ FK +GPTAMVGDG+N
Sbjct: 549 LGIKTAMLTGDNQAAAMHAQEQLGNVLDVVHGDLLPEDKSRIIQEFKKEGPTAMVGDGVN 608

Query: 607 DTPALATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHAKVVQNVILSI 666
           D PALATADIGISMG+SGSALAT+TGN+ILMSND+R+IP+A+KLA+++  KVV+NV LSI
Sbjct: 609 DAPALATADIGISMGISGSALATQTGNIILMSNDIRRIPQAVKLARRARRKVVENVCLSI 668

Query: 667 GTKTAILGLAFAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHRHGKKCCKSSKLPCFS 726
             K  IL LAFAGHPL+WAAVL DVGTCLLVI NSMLLLR       KKC ++S      
Sbjct: 669 ILKAGILALAFAGHPLIWAAVLVDVGTCLLVIFNSMLLLREKKKIGNKKCYRAS------ 728

Query: 727 TKHGKCNSSNGRSSHRHDHHHDDHRCHVVDDRSSSRENRVHKHCCSEKNNVPQQKENKFH 786
                 +  NGR     D +  D    ++   + S   +    CC +K N    +EN   
Sbjct: 729 -----TSKLNGRKLEGDDDYVVDLEAGLL---TKSGNGQCKSSCCGDKKN----QENVVM 788

Query: 787 HNPSNQCEKNQVEKESNGDSSRK-----VAESNCNCHSHHVAIDIHEDTDCERVEH 838
             PS++   +       GD   +     V +  C+  +     D+   + C++  H
Sbjct: 789 MKPSSKTSSDHSHPGCCGDKKEEKVKPLVKDGCCSEKTRKSEGDMVSLSSCKKSSH 822

BLAST of Lcy08g012590 vs. TAIR 10
Match: AT4G30110.1 (heavy metal atpase 2 )

HSP 1 Score: 957.2 bits (2473), Expect = 8.6e-279
Identity = 516/850 (60.71%), Postives = 648/850 (76.24%), Query Frame = 0

Query: 12  MRENKLQRSHFDVLGLCCSSEVPLIENILKPLEGIKDISVIVPTRTLIVVHDSLLISQLQ 71
           M   K+ +S+FDVLG+CC+SEVPLIENIL  ++G+K+ SVIVP+RT+IVVHD+L++SQ Q
Sbjct: 1   MASKKMTKSYFDVLGICCTSEVPLIENILNSMDGVKEFSVIVPSRTVIVVHDTLILSQFQ 60

Query: 72  IVKALNEARLEANVQLNGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLQWLAVAAVAA 131
           IVKALN+A+LEANV++ G+   K KWPSPFA+ SG+LL  SF KY+Y P +WLAVAAV A
Sbjct: 61  IVKALNQAQLEANVRVTGETNFKNKWPSPFAVVSGILLLLSFFKYLYSPFRWLAVAAVVA 120

Query: 132 GIFPILLKAVSAVRHLRIDVNILAIIAVVGTIAMNDYMEAGSIVFLFSIAEWLESRASHK 191
           GI+PIL KAV+++   RID+NIL ++ V  TI M DY EA  +VFLF+IAEWL+SRAS+K
Sbjct: 121 GIYPILAKAVASLARFRIDINILVVVTVGATIGMQDYTEAAVVVFLFTIAEWLQSRASYK 180

Query: 192 ANAAMSSLMSLAPQTATIAETGEIVDSKEVKLNTVLAVKAGEVIPIDGIVVEGKCEVDEK 251
           A+A M SLMSLAPQ A IAETGE V+  E+K NTV+AVKAGE IPIDG+VV+G CEVDEK
Sbjct: 181 ASAVMQSLMSLAPQKAVIAETGEEVEVDELKTNTVIAVKAGETIPIDGVVVDGNCEVDEK 240

Query: 252 TLTGESFPVAKQKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAELVEEAQNNKSKTQR 311
           TLTGE+FPV K KDS VWAGTINLNGYI+V TT +AEDCVVAKMA+LVEEAQN+K++TQR
Sbjct: 241 TLTGEAFPVPKLKDSTVWAGTINLNGYITVNTTALAEDCVVAKMAKLVEEAQNSKTETQR 300

Query: 312 FIDECAKYYTPAVLVISACLAAIPAALRVHNLSHWFHLALVVLVSACPCALILSTPVAAF 371
           FID+C+KYYTPA+++IS C  AIP AL+VHNL HW HLALVVLVSACPC LILSTPVA F
Sbjct: 301 FIDKCSKYYTPAIILISICFVAIPFALKVHNLKHWVHLALVVLVSACPCGLILSTPVATF 360

Query: 372 CALTKAAMAGVLIKGGDHLELLAKIKVMAFDKTGTITRGEFVVTDFRALCDNISLHTLLH 431
           CALTKAA +G+LIKG D+LE LAKIK++AFDKTGTITRGEF+V DF++L ++ISL +LL+
Sbjct: 361 CALTKAATSGLLIKGADYLETLAKIKIVAFDKTGTITRGEFIVMDFQSLSEDISLQSLLY 420

Query: 432 WVSSIESKSSHPMAAALVNHGKFLSVDIKPENVEEFENFPGEGVRGKIDGNNIYIGSRKI 491
           WVSS ESKSSHPMAAA+V++ + +SV+ KPE VE+++NFPGEG+ GKIDG  +YIG+++I
Sbjct: 421 WVSSTESKSSHPMAAAVVDYARSVSVEPKPEAVEDYQNFPGEGIYGKIDGKEVYIGNKRI 480

Query: 492 AARAGCPTAPSSDDDETKQGQTVGYVFCGTTLAGSFGLSDACRSGVREAIQEIKSFGIKT 551
           A+RAGC + P  D D TK G+T+GYV+ G TLAG F LSDACRSGV +A++E+KS GIK 
Sbjct: 481 ASRAGCLSVPDIDVD-TKGGKTIGYVYVGETLAGVFNLSDACRSGVAQAMKELKSLGIKI 540

Query: 552 AMLTGDCNAAAMHAQQQLGNALDVIHSELLPKEKANIIKRFK-GDGPTAMVGDGLNDTPA 611
           AMLTGD +AAAMHAQ+QLGNA+D++ +ELLP++K+ IIK+ K  +GPTAMVGDGLND PA
Sbjct: 541 AMLTGDNHAAAMHAQEQLGNAMDIVRAELLPEDKSEIIKQLKREEGPTAMVGDGLNDAPA 600

Query: 612 LATADIGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHAKVVQNVILSIGTKT 671
           LATADIGISMGVSGSALATETGN+ILMSND+R+IP+AIKLAK++  KVV+NV++SI  K 
Sbjct: 601 LATADIGISMGVSGSALATETGNIILMSNDIRRIPQAIKLAKRAKRKVVENVVISITMKG 660

Query: 672 AILGLAFAGHPLVWAAVLADVGTCLLVILNSMLLLRGTDHRHGKKCCKSSK--------- 731
           AIL LAFAGHPL+WAAVLADVGTCLLVILNSMLLL    H+ G KC + S          
Sbjct: 661 AILALAFAGHPLIWAAVLADVGTCLLVILNSMLLL-SDKHKTGNKCYRESSSSSVLIAEK 720

Query: 732 -------------LPCFSTKH---GKCNSSNGRSSHRHDHHHDDH-RCHVVDDRSSSREN 791
                        LP  S KH   G C +     + +      DH      + +      
Sbjct: 721 LEGDAAGDMEAGLLPKISDKHCKPGCCGTKTQEKAMKPAKASSDHSHSGCCETKQKDNVT 780

Query: 792 RVHKHCCSEKNNVPQQKENKFHHNPSNQCEKNQVEKESNGDSSRKVAESNC------NCH 829
            V K CC+E  ++    ++    + S Q  +++V+ + +  +     +S C        H
Sbjct: 781 VVKKSCCAEPVDLGHGHDSGCCGDKSQQPHQHEVQVQQSCHNKPSGLDSGCCGGKSQQPH 840

BLAST of Lcy08g012590 vs. TAIR 10
Match: AT4G30120.1 (heavy metal atpase 3 )

HSP 1 Score: 678.3 bits (1749), Expect = 7.7e-195
Identity = 343/542 (63.28%), Postives = 440/542 (81.18%), Query Frame = 0

Query: 6   MEKEKVMRENKLQRSHFDVLGLCCSSEVPLIENILKPLEGIKDISVIVPTRTLIVVHDSL 65
           M + +  ++  LQ S+FDV+G+CCSSEV ++ N+L+ ++G+K+ SVIVP+RT+IVVHD+ 
Sbjct: 1   MAEGEESKKMNLQTSYFDVVGICCSSEVSIVGNVLRQVDGVKEFSVIVPSRTVIVVHDTF 60

Query: 66  LISQLQIVKALNEARLEANVQLNGKGISKKKWPSPFAIASGLLLTASFLKYVYHPLQWLA 125
           LIS LQIVKALN+ARLEA+V+  G+   K +WPSPFAI SG+LL  SF KY Y PL+WLA
Sbjct: 61  LISPLQIVKALNQARLEASVRPYGETSLKSQWPSPFAIVSGVLLVLSFFKYFYSPLEWLA 120

Query: 126 VAAVAAGIFPILLKAVSAVRHLRIDVNILAIIAVVGTIAMNDYMEAGSIVFLFSIAEWLE 185
           + AV AG+FPIL KAV++V   R+D+N L +IAV+ T+ M D+ EA +IVFLFS+A+WLE
Sbjct: 121 IVAVVAGVFPILAKAVASVTRFRLDINALTLIAVIATLCMQDFTEAATIVFLFSVADWLE 180

Query: 186 SRASHKANAAMSSLMSLAPQTATIAETGEIVDSKEVKLNTVLAVKAGEVIPIDGIVVEGK 245
           S A+HKA+  MSSLMSLAP+ A IA+TG  VD  EV +NTV++VKAGE IPIDG+VV+G 
Sbjct: 181 SSAAHKASIVMSSLMSLAPRKAVIADTGLEVDVDEVGINTVVSVKAGESIPIDGVVVDGS 240

Query: 246 CEVDEKTLTGESFPVAKQKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAELVEEAQNN 305
           C+VDEKTLTGESFPV+KQ++S V A TINLNGYI V+TT +A DCVVAKM +LVEEAQ +
Sbjct: 241 CDVDEKTLTGESFPVSKQRESTVMAATINLNGYIKVKTTALARDCVVAKMTKLVEEAQKS 300

Query: 306 KSKTQRFIDECAKYYTPAVLVISACLAAIPAALRVHNLSHWFHLALVVLVSACPCALILS 365
           ++KTQRFID+C++YYTPAV+V +AC A IP  L+V +LSHWFHLALVVLVS CPC LILS
Sbjct: 301 QTKTQRFIDKCSRYYTPAVVVSAACFAVIPVLLKVQDLSHWFHLALVVLVSGCPCGLILS 360

Query: 366 TPVAAFCALTKAAMAGVLIKGGDHLELLAKIKVMAFDKTGTITRGEFVVTDFRALCDNIS 425
           TPVA FCALTKAA +G LIK GD LE LAKIK++AFDKTGTIT+ EF+V+DFR+L  +I+
Sbjct: 361 TPVATFCALTKAATSGFLIKTGDCLETLAKIKIVAFDKTGTITKAEFMVSDFRSLSPSIN 420

Query: 426 LHTLLHWVSSIESKSSHPMAAALVNHGKFLSVDIKPENVEEFENFPGEGVRGKIDGNNIY 485
           LH LL+WVSSIE KSSHPMAAAL+++ + +SV+ KP+ VE F+NFPGEGV G+IDG +IY
Sbjct: 421 LHKLLYWVSSIECKSSHPMAAALIDYARSVSVEPKPDIVENFQNFPGEGVYGRIDGQDIY 480

Query: 486 IGSRKIAARAGCPTAPSSDDDET-KQGQTVGYVFCGTTLAGSFGLSDACRSGVREAIQEI 545
           IG+++IA RAGC T    D + T K+G+T+GY++ G  L GSF L D CR GV +A++E+
Sbjct: 481 IGNKRIAQRAGCLTDNVPDIEATMKRGKTIGYIYMGAKLTGSFNLLDGCRYGVAQALKEL 540

Query: 546 KS 547
           KS
Sbjct: 541 KS 542

BLAST of Lcy08g012590 vs. TAIR 10
Match: AT1G63440.1 (heavy metal atpase 5 )

HSP 1 Score: 246.9 bits (629), Expect = 5.8e-65
Identity = 183/578 (31.66%), Postives = 302/578 (52.25%), Query Frame = 0

Query: 167 DYMEAGSIVFLFSI-AEWLESRASHKANAAMSSLMSLAPQTATIAE-------TG-EIVD 226
           D+ E  +++  F I  ++LE  A  K + A++ LM+LAP TA +         TG E +D
Sbjct: 404 DFFETSAMLISFIILGKYLEVMAKGKTSQAIAKLMNLAPDTAILLSLDKEGNVTGEEEID 463

Query: 227 SKEVKLNTVLAVKAGEVIPIDGIVVEGKCEVDEKTLTGESFPVAKQKDSLVWAGTINLNG 286
            + ++ N V+ +  G  +  DG V+ G+  V+E  +TGE+ PVAK+K   V  GT+N NG
Sbjct: 464 GRLIQKNDVIKIVPGAKVASDGYVIWGQSHVNESMITGEARPVAKRKGDTVIGGTLNENG 523

Query: 287 YISVQTTVVAEDCVVAKMAELVEEAQNNKSKTQRFIDECAKYYTPAVLVISACL------ 346
            + V+ T V  +  +A++  LVE AQ  K+  Q+  D  +K++ P V+ +S         
Sbjct: 524 VLHVKVTRVGSESALAQIVRLVESAQLAKAPVQKLADRISKFFVPLVIFLSFSTWLAWFL 583

Query: 347 ---------AAIPAALRVHNLSHWFHLALVVLVSACPCALILSTPVAAFCALTKAAMAGV 406
                    + IP+++    L+  F ++  V+V ACPCAL L+TP A        A  GV
Sbjct: 584 AGKLHWYPESWIPSSMDSFELALQFGIS--VMVIACPCALGLATPTAVMVGTGVGASQGV 643

Query: 407 LIKGGDHLELLAKIKVMAFDKTGTITRGEFVVTDFRALCDNISLHTLLHWVSSIESKSSH 466
           LIKGG  LE   K+  + FDKTGT+T G+ VV   + L  N+ L      V++ E  S H
Sbjct: 644 LIKGGQALERAHKVNCIVFDKTGTLTMGKPVVVKTK-LLKNMVLREFYELVAATEVNSEH 703

Query: 467 PMAAALVNHG-KFLSVDIKPENVE--EFENFPGEGVRGKIDGNNIYIGSRKIAARAGCPT 526
           P+A A+V +  KF   +  P   E  +F +  G+GV+  + G  I +G++ +        
Sbjct: 704 PLAKAIVEYAKKFRDDEENPAWPEACDFVSITGKGVKATVKGREIMVGNKNLMNDHKVII 763

Query: 527 APSSDD---DETKQGQTVGYVFCGTTLAGSFGLSDACRSGVREAIQEIKSFGIKTAMLTG 586
              +++   D     QT   V   + L G   +SD  +   REAI  +KS  IK+ M+TG
Sbjct: 764 PDDAEELLADSEDMAQTGILVSINSELIGVLSVSDPLKPSAREAISILKSMNIKSIMVTG 823

Query: 587 DCNAAAMHAQQQLGNALDVIHSELLPKEKANIIKRFKGDG-PTAMVGDGLNDTPALATAD 646
           D    A    +++G  +D + +E  P++KA  +K  +  G   AMVGDG+ND+PAL  AD
Sbjct: 824 DNWGTANSIAREVG--IDSVIAEAKPEQKAEKVKELQAAGHVVAMVGDGINDSPALVAAD 883

Query: 647 IGISMGVSGSALATETGNVILMSNDMRKIPKAIKLAKKSHAKVVQNVILSIGTKTAILGL 705
           +G+++G +G+ +A E  +++LM +++  +  AI L++K+ +++  N + ++G    ++G+
Sbjct: 884 VGMAIG-AGTDIAIEAADIVLMKSNLEDVITAIDLSRKTFSRIRLNYVWALGYN--LMGI 943

BLAST of Lcy08g012590 vs. TAIR 10
Match: AT5G44790.1 (copper-exporting ATPase / responsive-to-antagonist 1 / copper-transporting ATPase (RAN1) )

HSP 1 Score: 238.0 bits (606), Expect = 2.7e-62
Identity = 227/800 (28.38%), Postives = 383/800 (47.88%), Query Frame = 0

Query: 3   EEAMEKEKVMRENKLQRSHFDVLGLCCSSEVPLIENILKPLEGIKDISVIVPTRTLIVVH 62
           E+A  +  +++ N+  +    V G+    +  ++E IL  L G++   +   +  L VV 
Sbjct: 192 EDAGFEGSLVQSNQQDKLVLRVDGILNELDAQVLEGILTRLNGVRQFRLDRISGELEVVF 251

Query: 63  DSLLISQLQIVKALNE---ARLEANV-----QLNGKGISKKKWPSPFAIASGLLLTASFL 122
           D  ++S   +V  + E    + +  V     +L+ K   +        I+S +L    F 
Sbjct: 252 DPEVVSSRSLVDGIEEDGFGKFKLRVMSPYERLSSKDTGEASNMFRRFISSLVLSIPLFF 311

Query: 123 KYVYHPL------------------QWLAVAAVAAGIFPILLK-AVSAVRHLRIDVNILA 182
             V  P                    WL  A V+   F I  +  V+A R LR     + 
Sbjct: 312 IQVICPHIALFDALLVWRCGPFMMGDWLKWALVSVIQFVIGKRFYVAAWRALRNGSTNMD 371

Query: 183 IIAVVGTIA------------------MNDYMEAGSIVFLF-SIAEWLESRASHKANAAM 242
           ++  +GT A                     Y +A +++  F  + ++LES A  K + AM
Sbjct: 372 VLVALGTSASYFYSVGALLYGAVTGFWSPTYFDASAMLITFVLLGKYLESLAKGKTSDAM 431

Query: 243 SSLMSLAPQTA---TIAETGEIVDSKE-----VKLNTVLAVKAGEVIPIDGIVVEGKCEV 302
             L+ L P TA   T  + G++V  +E     ++    L V  G  IP DG+VV G   V
Sbjct: 432 KKLVQLTPATAILLTEGKGGKLVGEREIDALLIQPGDTLKVHPGAKIPADGVVVWGSSYV 491

Query: 303 DEKTLTGESFPVAKQKDSLVWAGTINLNGYISVQTTVVAEDCVVAKMAELVEEAQNNKSK 362
           +E  +TGES PV+K+ DS V  GTIN++G + ++ T V  D V++++  LVE AQ +K+ 
Sbjct: 492 NESMVTGESVPVSKEVDSPVIGGTINMHGALHMKATKVGSDAVLSQIISLVETAQMSKAP 551

Query: 363 TQRFIDECAKYYTPAVLV----------ISACLAAIPAALRVHNLSHW---FHLALVVLV 422
            Q+F D  A  + P V+           I   + A P      N +H+      ++ V+V
Sbjct: 552 IQKFADYVASIFVPVVITLALFTLVGWSIGGAVGAYPDEWLPENGTHFVFSLMFSISVVV 611

Query: 423 SACPCALILSTPVAAFCALTKAAMAGVLIKGGDHLELLAKIKVMAFDKTGTITRGEFVVT 482
            ACPCAL L+TP A   A    A  GVLIKGGD LE   K+K + FDKTGT+T+G+  VT
Sbjct: 612 IACPCALGLATPTAVMVATGVGATNGVLIKGGDALEKAHKVKYVIFDKTGTLTQGKATVT 671

Query: 483 DFRALCDNISLHTLLHWVSSIESKSSHPMAAALVNHGK-FLSVDIKPENVE--------- 542
             +   + +     L  V+S E+ S HP+A A+V + + F   D   E+ E         
Sbjct: 672 TTKVFSE-MDRGEFLTLVASAEASSEHPLAKAIVAYARHFHFFDESTEDGETNNKDLQNS 731

Query: 543 -------EFENFPGEGVRGKIDGNNIYIGSRKIAARAGCPTAPSSD---DDETKQGQTVG 602
                  +F   PG+G++  ++   I +G+RK+ +          +   +D  + G+T  
Sbjct: 732 GWLLDTSDFSALPGKGIQCLVNEKMILVGNRKLMSENAINIPDHVEKFVEDLEESGKTGV 791

Query: 603 YVFCGTTLAGSFGLSDACRSGVREAIQEIKSFGIKTAMLTGDCNAAAMHAQQQLGNALDV 662
            V     L G  G++D  +      ++ +   G++  M+TGD    A    +++G  ++ 
Sbjct: 792 IVAYNGKLVGVMGIADPLKREAALVVEGLLRMGVRPIMVTGDNWRTARAVAKEVG--IED 851

Query: 663 IHSELLPKEKANIIKRFKGDGPT-AMVGDGLNDTPALATADIGISMGVSGSALATETGNV 707
           + +E++P  KA++I+  + DG T AMVGDG+ND+PALA AD+G+++G +G+ +A E  + 
Sbjct: 852 VRAEVMPAGKADVIRSLQKDGSTVAMVGDGINDSPALAAADVGMAIG-AGTDVAIEAADY 911