Lcy08g006080 (gene) Sponge gourd (P93075) v1

Overview
NameLcy08g006080
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionDiacylglycerol O-acyltransferase
LocationChr08: 7702348 .. 7706711 (-)
RNA-Seq ExpressionLcy08g006080
SyntenyLcy08g006080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAATGGAAATCAAGGAAGATGAGTTGCTTGAGCCTGTTAGCCCTACTGCTCAATGCTTCAACAGCTCTGTATCGTCCATTTCTGTTATTGTTGTTTTGGAATTTGAAACTTCCATTGATGATTCCGAAATTACATCTTTTGCTATGGATCTCATCCCTCTCAACCCGCAGTTTAGTTCCATTATGGTACTTTCTCTCAACCCACTACTCTCTAGTTCTTTATAGTATTGTGTTTCTTTTGTCCTGAGTCCAACGACATTTTGAAATGAGGATTCGAACCTTTGACCTTTTAGTTGAAGATACATGTCGATTACTTCTGAGTTATGCTTAGTTTGGTTGGTACTCTCCTCACTAAAAGCACTAATTTCTTACCTCATTATTATGGTCATTATATACTCTTCTTCTCTCTTTCTCTTAAAACACTAACGAAAACTAGGATTAGGGTTTTTTTCTTCCTCTCGTGCTTTCAACATAGCCATTATTATTAGGTTAAATGGTAAGTTTGGTCTTTGAGGTGAGATAATAATATTCCAAACTTTAAAAGCGTTTAATAGGTTTTAATTAATTGAACTTTAAATATTAGGTGTAGGAAGTACCCATTTTTTTTTTTTTTTTGAAATCAACAATGAGGTATGAAGAATTGAACCTCTAACGTCTTGGCTGAAAGCATATGTTTTAACCAGTTAAGTTACATTTAAGTCGGCGTGACGAAGTATTAAAACTTTAAAAAATATCTGATAATCCCTTAGACTTTTTTTTTTTAAAAAAAAAAAAAAAGTGTCATAAATCAAAGTCCTATTTATTATACAAAAAATTGAAAGCTAAGAGCTTATTAGATGCAAAGTTAAAAGTTAAGAATTTATTATTAAAAATTCAACAGCTTATATAGATACAATATTGGAAGTTTATAAATATTAGACACAGCGCTCAAAGTCTATGGGTCAAACTTATAATTTAACCTTATTTTTGTTATGATTCCTCTAAAATTTATTCACAAAAATTTATTTTGGAAACAGGTCAATGACATAAATGGGGAGAGAAAATGGAAGAAAGTTGAAGTGAATCTTGAACAACATATTCTTGTGCCGACTTTACCCTCAAACTTGTCACTTGAACACTTTGACGCATATTTCAATGAATACATGATAAATTTGGCTCTACAAGAGTTACCCCAAAACAAACCATTGTGGGAAATTCATATAATAAACCATCCAACTAGTAAATCAATTGGGAATCTTATTTTGAAGCATCACCATTCACTTGGAGATGGCTATTCCATGATGGGTCTTCTTATTTCTAGCTTAACAAGAGCAGACAATCCTTCTCTTCCCTTAACTTTTCCTTCGAGGAAGTTCTCGAAGCAACCGGGGCACGTTCTAAGTCGTGTGTCTCGATTTTTTCTCTCATCTATCAATAGTGTATTAGATTTTGGATGGAGCATAATTAAAAGCACTGTTCTTGAAGATGACTTGACACCTATAAGATCAGGTGGAGATGGTGTGGAGTTAAGGCCAATTGCAATATCGACAATGACATTATCTCTTGATCCAATCAAACAAATCAAATCCAAACTTAGAGTGGTATGATTTCCATTTGTTTCTTTATATGTGGGTGTGATTATCTACTTTTGACCATGGTTGAATGACTTGGGATATTCTTTTACAAAAGGATTTGATTGAAATAAATGATTACAAATAGAAATTAAACTAACATATTTCATAGGAATCCTTAACATATTTAGCCATTGATGTAAATCATAAGAATGTGGATAATTGACAAGACTCTTTTTTTTTTTTTTTATACAACACCATAAGATTTGAACCTCTAACTTCTTGATTAGGTATATATGTCTTAGATAGTTGAGACATTACTCATGTTGACTAATGATAACGAAATCTTGGGATTATGGAAATAGATCACCAACTAGCTTAATGGATTATAGTTAGTAAATTAAAGTAGGATTTGATGATAGTATATATGACAATCTTATCCTTCCTATCTTATTTTATTGTTTTTTTAAGAAAGAAGATATGCAGCCATGTATTGATCATAGGTAGTGTTGATATGTCTTGAATATGATATGTTAGGGTTAAGAGTGGTGTCATATATAAACCCTCTTAACCTATTTTGTCCTAATCTCTGTAATCTGCATACGTTTTACTCCTCACTAATAATTAAGATCGTTCTCTCCCTCTGCCCGTGGACGTAGCTAACACATCGTTAGTGAACCATGTAAATCTTTGTGTGTTGTTTCTGTCTTAACAGGTAGAGTCCTAGGGTTAATGAATTTCTATTTAACATTGTTAATTTGCAAACGATCAACGATGTGGTTACGGGAATGATTTTTTTGGGACTTCGACTATACATGCAAAAGATTTGTCCCGACTCAACCCAATCCAACTCAACTGCATTGGTTTTGCTCAACACAAGGATGTTTGGAAATTACAAGTCTATACAAGATATGCTTAAGCCGAATTCAGATGCCCCATGGGGGAATCGATTTGCCCTCTGCAATTCATCAAAGGCGCACATAAACTTATCAATAGGAAGAGAAACTCTTCTGTTGTTTTCCTCGTTGGCAAGCTTATGGAGATGATACACAAGTTTAGAGGCCCTGAGGTAAGTATACAAAAAAGTGAAACATATTGAATAGTTCTGGTCCAATGCTTTCGGTAAATCTTAAAATTAGTTAGGCCTCGTTTGATAACCATTTTGTTTTTGGTTTTTGGTTTTTGAAAATTAAGCCTATAAACACTACTTTCATTAATAAGTTTCTTTGTTTTGTTCTCTACCTTCTTCCCATGTTTTCAAAAACGAACTCAAGTTTTGAAAACTAAAAAAAATATAGTTTCCAAAAACTTGTTTTTGTTTTTGGAATTTGGCTAGGAATTCAAGTGTGTCATTGACAAGGATGAAAACAATGGTAAGAAATTTGGGAGAAAATAAGTTTAATTTTCAAAAATCAAAAATAAAAAATGAAATGGTTATCAAACGGGGCCTTATTATTTTTTTTTTTATAAAAAAAAGTCTATCATCCTACCATCTATAAAATTTTGAAAACATATTCATGTTTGGTATCTTTTCTTGAATGGAATTAATTAATATTAATATTTTACAAAGTCAGATAAAAATTAACTTTAAAATTAACAAGGATATGACTAATTTTGAAGTTTATTAGAAGTAGAAGGACTATATATATTTCAGATCAAAATAGTATTTTAATAATTTAGTCTTATTTAAAAATACTTATCTATACATTTGCCAATTATCAAAGCTCCTGTTAATCCTATGGTGTGCTTTTATTGATTTGTTATTTAAATATAGGGAAAAATACACTTTTAGTCCCTGAGGTTTGGGTTAGGTTTCATTTTGGTCCCTGAGTTTTCAAAGTCAACAATTTAGTCCTTGAGGTTTAGTTTTAGTTTCATTTTAGTCCCTCCATCCATTTTTCCGTTAAAAACTAACGGTTTGCTGACATGGCACCTATTTTTTTAAAAAATTATATATTTTTAATTTTTAAAATAAAAAATAATATTAAATTAATCAAAATATATTTTTTTATTCTTTCTTCTTCTTCAAGTTGTGCACAGTTCCGCCGCTGGTCATCGTTGTCCATCGCCGCCGGTTACTGCCGGCCGCCGGAGATGTTTTTTCTATTTTTTTTCCTTTTTTCTTTCTCTGCCCCTCTCTTCATCTCATCTCTCCCGCCAGCGGCCCGAGCATTTTCTCCGTGCGAAGCCTAGCCTGATTATCTCTCCGGTGAACCATCACGAGCAGATCTGGGTTTCCGGCAGGTCCTTCACGTCGACGTTCACTGTGCAGGAGCTCCAAACGACCCTCGATCGGCGATACCCACGACCAGACGAAGATCCGCACACCCGTCTTTGCTCGCAGCTCGCGTGAACCCGTCGTCAAAGTCTAAAAACCAGATCTGGAACGCCCGTTTCACACTCATCTTCGCTCAGATCTGGAACATCACTCGCAATCGTTTCACACTCGCAGCTGCACATATCTGAAACTCCATTTCCTTTTCGTTCCGACAGTGCAGATCTGGGAGAAGAAGACGACTCGTGTCGCCGGAACCTTCAGAAATCGCCGATAGACTAGTGGGTCGTGGGTCTTGCGGTGAGAGAGGGACGATGCGAGAGAGAGGGTCGTGGGTATGTGAGAGAAAAGGACGCGTGGGTCGTGGGTCTTGCGTAGCCGTGGCCAAAGCTGCTCGCCGGAGACAGAAGAAGACCAGAATAAGAACCACCACGGTCTGTGCTCGTCGGAAGAGACGCAGTAAGAAAAATCAGGAGGGGAAGCAACAACCACGGCCGGAGCTACTCGCCGGAGGGGAAGAAGACCGGAATTGTTGA

mRNA sequence

ATGGCAATGGAAATCAAGGAAGATGAGTTGCTTGAGCCTGTTAGCCCTACTGCTCAATGCTTCAACAGCTCTGTATCGTCCATTTCTGTTATTGTTGTTTTGGAATTTGAAACTTCCATTGATGATTCCGAAATTACATCTTTTGCTATGGATCTCATCCCTCTCAACCCGCAGTTTAGTTCCATTATGGTCAATGACATAAATGGGGAGAGAAAATGGAAGAAAGTTGAAGTGAATCTTGAACAACATATTCTTGTGCCGACTTTACCCTCAAACTTGTCACTTGAACACTTTGACGCATATTTCAATGAATACATGATAAATTTGGCTCTACAAGAGTTACCCCAAAACAAACCATTGTGGGAAATTCATATAATAAACCATCCAACTAGTAAATCAATTGGGAATCTTATTTTGAAGCATCACCATTCACTTGGAGATGGCTATTCCATGATGGGTCTTCTTATTTCTAGCTTAACAAGAGCAGACAATCCTTCTCTTCCCTTAACTTTTCCTTCGAGGAAGTTCTCGAAGCAACCGGGGCACGTTCTAAGTCGTGTGTCTCGATTTTTTCTCTCATCTATCAATAGTGTATTAGATTTTGGATGGAGCATAATTAAAAGCACTGTTCTTGAAGATGACTTGACACCTATAAGATCAGGTGGAGATGGTGTGGAGTTAAGGCCAATTGCAATATCGACAATGACATTATCTCTTGATCCAATCAAACAAATCAAATCCAAACTTAGAGTGTGCAGATCTGGGAGAAGAAGACGACTCGTGTCGCCGGAACCTTCAGAAATCGCCGATAGACTAGTGGGTCGTGGGTCTTGCGGTGAGAGAGGGACGATGCGAGAGAGAGGGTCGTGGGTATGTGAGAGAAAAGGACGCGTGGGTCGTGGGTCTTGCGTAGCCGTGGCCAAAGCTGCTCGCCGGAGACAGAAGAAGACCAGAATAAGAACCACCACGGTCTGTGCTCGTCGGAAGAGACGCAGTAAGAAAAATCAGGAGGGGAAGCAACAACCACGGCCGGAGCTACTCGCCGGAGGGGAAGAAGACCGGAATTGTTGA

Coding sequence (CDS)

ATGGCAATGGAAATCAAGGAAGATGAGTTGCTTGAGCCTGTTAGCCCTACTGCTCAATGCTTCAACAGCTCTGTATCGTCCATTTCTGTTATTGTTGTTTTGGAATTTGAAACTTCCATTGATGATTCCGAAATTACATCTTTTGCTATGGATCTCATCCCTCTCAACCCGCAGTTTAGTTCCATTATGGTCAATGACATAAATGGGGAGAGAAAATGGAAGAAAGTTGAAGTGAATCTTGAACAACATATTCTTGTGCCGACTTTACCCTCAAACTTGTCACTTGAACACTTTGACGCATATTTCAATGAATACATGATAAATTTGGCTCTACAAGAGTTACCCCAAAACAAACCATTGTGGGAAATTCATATAATAAACCATCCAACTAGTAAATCAATTGGGAATCTTATTTTGAAGCATCACCATTCACTTGGAGATGGCTATTCCATGATGGGTCTTCTTATTTCTAGCTTAACAAGAGCAGACAATCCTTCTCTTCCCTTAACTTTTCCTTCGAGGAAGTTCTCGAAGCAACCGGGGCACGTTCTAAGTCGTGTGTCTCGATTTTTTCTCTCATCTATCAATAGTGTATTAGATTTTGGATGGAGCATAATTAAAAGCACTGTTCTTGAAGATGACTTGACACCTATAAGATCAGGTGGAGATGGTGTGGAGTTAAGGCCAATTGCAATATCGACAATGACATTATCTCTTGATCCAATCAAACAAATCAAATCCAAACTTAGAGTGTGCAGATCTGGGAGAAGAAGACGACTCGTGTCGCCGGAACCTTCAGAAATCGCCGATAGACTAGTGGGTCGTGGGTCTTGCGGTGAGAGAGGGACGATGCGAGAGAGAGGGTCGTGGGTATGTGAGAGAAAAGGACGCGTGGGTCGTGGGTCTTGCGTAGCCGTGGCCAAAGCTGCTCGCCGGAGACAGAAGAAGACCAGAATAAGAACCACCACGGTCTGTGCTCGTCGGAAGAGACGCAGTAAGAAAAATCAGGAGGGGAAGCAACAACCACGGCCGGAGCTACTCGCCGGAGGGGAAGAAGACCGGAATTGTTGA

Protein sequence

MAMEIKEDELLEPVSPTAQCFNSSVSSISVIVVLEFETSIDDSEITSFAMDLIPLNPQFSSIMVNDINGERKWKKVEVNLEQHILVPTLPSNLSLEHFDAYFNEYMINLALQELPQNKPLWEIHIINHPTSKSIGNLILKHHHSLGDGYSMMGLLISSLTRADNPSLPLTFPSRKFSKQPGHVLSRVSRFFLSSINSVLDFGWSIIKSTVLEDDLTPIRSGGDGVELRPIAISTMTLSLDPIKQIKSKLRVCRSGRRRRLVSPEPSEIADRLVGRGSCGERGTMRERGSWVCERKGRVGRGSCVAVAKAARRRQKKTRIRTTTVCARRKRRSKKNQEGKQQPRPELLAGGEEDRNC
Homology
BLAST of Lcy08g006080 vs. ExPASy Swiss-Prot
Match: Q94CK0 (Wax ester synthase/diacylglycerol acyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=WSD7 PE=1 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 2.4e-25
Identity = 78/251 (31.08%), Postives = 133/251 (52.99%), Query Frame = 0

Query: 12  EPVSPTAQCFNSSVSSISVIVVLEFETSIDDSEITSFAMDLIPLNPQFSSIMVNDINGER 71
           EPVSP A+ F S    +  + ++ F+T I+   +       +  +P+FSSI+ +  NG  
Sbjct: 7   EPVSPMARVFQSPGIDLCAVTIMGFKTKINPDVVLDALKQNVSKHPRFSSILSD--NG-A 66

Query: 72  KWKKVEVNLEQHILVPTLPSNLSLEHFDAYFNEYMINLALQELPQNKPLWEIHIINHPTS 131
           KW + EVN+E H++VP + +    E   ++ ++YM  L +  L +++PLW+IHI+N  TS
Sbjct: 67  KWIETEVNVEDHVIVPYIDAEEIGEGGQSFIDDYMSRLTMIPLDRSRPLWDIHILNVKTS 126

Query: 132 KSIGNLILKHHHSLGDGYSMMGLLISSLTRADNPSL-PLTFPSRKFSKQPGHVLSRVSRF 191
           ++     ++ HHSLGDG S++ L+++   +  +P +     PS K      H L     F
Sbjct: 127 EAEAVGFIRSHHSLGDGMSLISLMLACTHKTSDPDMFSNAIPSMKRRATMSHSLKTKGWF 186

Query: 192 FLS--SINSVLDFGWS------IIKSTV--LEDDLTPIRSGGDGVELRPIAISTMTLSLD 251
             S  +I S +   W+      ++ +TV  L+D  TP+++G D V   P       +SLD
Sbjct: 187 LRSIFTIGSTMRLLWNTTIDMLLLLATVLFLKDTKTPLKAGAD-VRSNPKRFYHRIISLD 246

BLAST of Lcy08g006080 vs. ExPASy Swiss-Prot
Match: Q9M3B2 (Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=WSD5 PE=2 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 3.5e-24
Identity = 78/269 (29.00%), Postives = 140/269 (52.04%), Query Frame = 0

Query: 4   EIKEDELLEPVSPTAQCFNSSVSSISVIVVLEFETSIDDSEITSFAMDLIPLNPQFSSIM 63
           E++E+E  +P+SP A+ F++   +  +I V+  +  I+   I       +  +P+FSS +
Sbjct: 20  EVEEEE--QPLSPAARLFHAPEFNCYIISVVGLKNKIEPDMIIEGIKQTLMRHPRFSSKL 79

Query: 64  VNDING---ERKWKKVEVNLEQHILVPTLPS-NLSLEHFDAYFNEYMINLALQELPQNKP 123
           VN+ N    E+KW +  V +E H+++P + + ++   + D +   Y+ +L    L  +KP
Sbjct: 80  VNNCNNNRQEQKWVRTNVVVEDHVIIPKIQTQHIENANADVFLESYVSDLTTIPLDTSKP 139

Query: 124 LWEIHIINHPTSKSIGNLILKHHHSLGDGYSMMGLLISSLTRADNPSLPLTFPSRKFSKQ 183
           LWE+H+++  TS +    +L+ HHSLGDG SMM L+++   +  NP+     PS  +  +
Sbjct: 140 LWEVHLLDLKTSDAENVAVLRIHHSLGDGMSMMSLVLACTRKTSNPN---ELPSLPYQNR 199

Query: 184 PGHVLSRV-------SRFF----------LSSINSVLDFGWSIIKSTVLEDDLTPIRSGG 243
           P    S +       SRFF          L  +N+V D    I  +  L+D  TPI+   
Sbjct: 200 PSSGSSSLKTSSRCYSRFFWLVMVLWSAALLVLNTVCDALEFIATALFLKDTETPIKGDF 259

Query: 244 DGVELRPIAISTMTLSLDPIKQIKSKLRV 252
              + + + +   T+SLD IK IK+ +++
Sbjct: 260 KLSKGKRMCMVHRTVSLDDIKLIKNAMKM 283

BLAST of Lcy08g006080 vs. ExPASy Swiss-Prot
Match: F4IU14 (Wax ester synthase/diacylglycerol acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=WSD3 PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 7.8e-24
Identity = 77/253 (30.43%), Postives = 128/253 (50.59%), Query Frame = 0

Query: 1   MAMEIKEDELLEPVSPTAQCFNSSVSSISVIVVLEFETSIDDSEITSFAMDLIPLNPQFS 60
           MA E +E   +EP+SP +Q F S      +I  L F+T  + S I     +     P+FS
Sbjct: 10  MAKEEQETAAIEPLSPVSQLFVSPSLYCFIIFTLGFQTRCNPSTIVEGVKNTWIKLPRFS 69

Query: 61  SIMVNDINGERKWKKVEVNLEQHILVPTLPSNLSLEHFDAYFNEYMINLALQELPQNKPL 120
           S +    NG+  W  V V +E H++VP L  + ++E+ D +  +Y   LA   +  ++PL
Sbjct: 70  SKVEIKKNGKASWVPVSVRVEDHVVVPDLDYS-NIENPDQFIEDYTSKLANTPMDMSRPL 129

Query: 121 WEIHIINHPTSKSIGNLILKHHHSLGDGYSMMGLLISSLTRADNP-SLPLTFPSRKFSKQ 180
           WE+H++N  TS +    I K HHSLGDG S++ LL++S  +  +P +LP T  +RK +  
Sbjct: 130 WELHLLNIKTSNAESLAIGKFHHSLGDGMSLISLLLASSRKTSDPDALPTTAATRKHASS 189

Query: 181 PGHVLSRVSRFFLS---SINSVLDFGWSIIKSTVLEDDLTPIRSGGDGVELRPIAISTMT 240
                  V RF+        +V++    ++    + D  TP+  G  G  +R   +    
Sbjct: 190 NKKSWWLVGRFWFMIRIIFTTVVELFKYLLTLCFMRDTKTPL-MGKTGDAIRSRKVIHRI 249

Query: 241 LSLDPIKQIKSKL 250
           +S D +K +K+ +
Sbjct: 250 VSFDDVKLVKNNM 260

BLAST of Lcy08g006080 vs. ExPASy Swiss-Prot
Match: Q9M3B3 (Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=WSD4 PE=2 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 2.9e-23
Identity = 73/262 (27.86%), Postives = 139/262 (53.05%), Query Frame = 0

Query: 6   KEDELLEPVSPTAQCFNSSVSSISVIVVLEFETSIDDSEITSFAMDLIPLNPQFSSIMVN 65
           KE+E  +P+SP A+ F++   + ++I V+ F++ +D         +    +P+FSS +V 
Sbjct: 14  KEEE--QPLSPAARLFHAPEFNCNIISVIGFKSKLDPCVFIRGFKESFIRHPRFSSKLVT 73

Query: 66  DINGE-RKWKKVEVNLEQHILVPTL-PSNLSLEHFDAYFNEYMINLALQELPQNKPLWEI 125
           D NG+ ++W +  V +E H +VP + P N  +E+ +A+  +Y+ +L    L  ++PLWE+
Sbjct: 74  DENGQNQRWVRTNVVVEDHFIVPKIKPQN--IENSNAFLEDYVSDLMKIPLDTSRPLWEL 133

Query: 126 HIINHPTSKSIGNLILKHHHSLGDGYSMMGLLISSLTRADNPSLPLTFPSRKFSKQPGHV 185
           H+++  TS +    +LK HHS+GDG S+M L+++ + +  NP    + P +  S     +
Sbjct: 134 HLLDLKTSDAENVAVLKIHHSVGDGMSIMSLVLACMRKTSNPDELPSLPYQYRSSSRSSL 193

Query: 186 LSRVSR--------------FFLSSINSVLDFGWSIIKSTVLEDDLTPIRSGGDGVELRP 245
           L+  SR                +  +N+V D    I+ +  ++D  TPI+      + + 
Sbjct: 194 LTTGSRSDSRLLWLVKVIWTAVILGLNTVCDALEFIVTTLFVKDTETPIKGDFLSTKSKQ 253

Query: 246 IAISTMTLSLDPIKQIKSKLRV 252
           + +   T+SLD IK  K+ + +
Sbjct: 254 LRLVHRTVSLDDIKLTKNAMNM 271

BLAST of Lcy08g006080 vs. ExPASy Swiss-Prot
Match: Q9C7H4 (Wax ester synthase/diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=WSD2 PE=2 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 1.1e-22
Identity = 75/257 (29.18%), Postives = 129/257 (50.19%), Query Frame = 0

Query: 1   MAMEIKEDELLEPVSPTAQCFNSSVSSISVIVVLEFETSIDDSEITSFAMDLIPLNPQFS 60
           MA+E +  E  EPVSP A+ F+     +  IV +  +T  + S I     + +  +P+FS
Sbjct: 1   MAIERQVTEAEEPVSPFARLFSLPGLDVFNIVTIGCKTEGNASTIVEGIKNTLINHPRFS 60

Query: 61  SIMV---NDINGERKWKKVEVNLEQHILVPTLPSNLSLEHFDAYFNEYMINLALQELPQN 120
           SI+V    +  G+ +W   ++N+E+H++VP +  N  +E+ D +  +Y  N+AL  +  +
Sbjct: 61  SILVTGHGEHKGKARWIPTKINVEEHVIVPDIDPN--IENPDEFLEDYTSNMALSPMDMS 120

Query: 121 KPLWEIHIINHPTSKSIGNLILKHHHSLGDGYSMMGLLISSLTRADNPSLPLTFPSRKFS 180
           KPLWE H++   TS +    + + HHSLGDG S+M LL++   +  +P    TF + K +
Sbjct: 121 KPLWEFHLLKLKTSHAEAVTVARFHHSLGDGMSLMSLLLACTRKTCDPEAFPTFVAPKKN 180

Query: 181 KQPGHVLSRVSRFFLSSINSVLDFGWSIIKSTVL---EDDLTPIRSGGDGVELRPIAIST 240
           K      S V+  +   +  +      +IKS V      D +    G  G  L       
Sbjct: 181 KAKNVCFSLVAWLWF-IVRLMFHTCVEVIKSIVFICRASDTSAHIMGKPGATLSANKFIH 240

Query: 241 MTLSLDPIKQIKSKLRV 252
             +SLD +K +K+ + +
Sbjct: 241 RIISLDDVKMVKNAMNM 254

BLAST of Lcy08g006080 vs. ExPASy TrEMBL
Match: A0A6J1GLS6 (Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455511 PE=4 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 8.2e-93
Identity = 179/250 (71.60%), Postives = 210/250 (84.00%), Query Frame = 0

Query: 3   MEIKEDELLEPVSPTAQCFNSSVSSISVIVVLEFETSIDDSEITSFAMDLIPLNPQFSSI 62
           ME+KEDELL+PVSPTAQ  NS V SIS+I VLEFE +ID+ +  SFA DLI LNP F+SI
Sbjct: 1   MEMKEDELLQPVSPTAQSMNSFVRSISLIAVLEFEIAIDEHKFMSFAKDLIHLNPLFTSI 60

Query: 63  MVNDINGERKWKKVEVNLEQHILVPTLPSNLSLEHFDAYFNEYMINLALQELPQNKPLWE 122
           MVND+NGERKWKKVEVN+E+HI+V T PSNLSL+ FDAYFNEYM N ALQELPQNKPLWE
Sbjct: 61  MVNDVNGERKWKKVEVNIEEHIIVHTPPSNLSLQLFDAYFNEYMTNYALQELPQNKPLWE 120

Query: 123 IHIINHPTSKSIGNLILKHHHSLGDGYSMMGLLISSLTRADNPSLPLTFPSRKFS-KQPG 182
           IHIIN PT+ + GNL+ K HHSLGDGYS+MG+LISS+TRA +PSLPLTFPSRK   +Q G
Sbjct: 121 IHIINCPTNNAAGNLVFKIHHSLGDGYSLMGVLISSMTRAHDPSLPLTFPSRKVKLEQSG 180

Query: 183 HVLSRVSRFFLSSINSVLDFGWSIIKSTVLEDDLTPIRSGGDGVELRPIAISTMTLSLDP 242
           H+L RV +F LSS+N+VLDFG +++KS+VLED LTPIRSGGDGVE R  AI T+T SL  
Sbjct: 181 HILGRVYQFLLSSMNTVLDFGGNMMKSSVLEDHLTPIRSGGDGVEFRTFAIRTITFSLHH 240

Query: 243 IKQIKSKLRV 252
           IK+IK+KL V
Sbjct: 241 IKKIKAKLGV 250

BLAST of Lcy08g006080 vs. ExPASy TrEMBL
Match: A0A0A0K749 (Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G051310 PE=4 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 1.8e-92
Identity = 184/255 (72.16%), Postives = 208/255 (81.57%), Query Frame = 0

Query: 6   KEDELLEPVSPTAQCFNSSVSSISVIVVLEFETSIDDSEITSFAMDLIPLNPQFSSIMVN 65
           +E+ELLEPVSPTAQCFNSS  SISVI VLEFE  ID+ +I  +A DLIPLNP FSSIMV+
Sbjct: 8   EEEELLEPVSPTAQCFNSSALSISVIAVLEFEIPIDEVQIMCYAKDLIPLNPLFSSIMVD 67

Query: 66  DINGERKWKKVEVNLEQHILVPTLPSNLSLE---HFDAYFNEYMINLALQELPQNKPLWE 125
           DINGERKWKKVEVNLE+HILVPT PSNLS+    ++DAYFN+Y+ NL  QEL +NKPLWE
Sbjct: 68  DINGERKWKKVEVNLEEHILVPTFPSNLSVVDDINYDAYFNKYITNLGAQELAKNKPLWE 127

Query: 126 IHIINHPTSKSIGNLILKHHHSLGDGYSMMGLLISSLTRADNPSLPLTFPSRKF------ 185
           IHII +PTS +  NLILK HHSLGDGYS+MGLLISS+ RADNPSLPLTFPSRK       
Sbjct: 128 IHIIKYPTSNAAANLILKFHHSLGDGYSVMGLLISSMKRADNPSLPLTFPSRKINSDDHN 187

Query: 186 SKQPGHVLSRVSRFFLSSINSVLDFGWSIIKSTVLEDDLTPIRSGGDGVELRPIAISTMT 245
           +K   HVL+ V  F    +NSVLDFGWSIIKS+VLEDDLTPIRSGGDGVE RP+AI T++
Sbjct: 188 NKNLRHVLNYVPSF----MNSVLDFGWSIIKSSVLEDDLTPIRSGGDGVEFRPVAICTIS 247

Query: 246 LSLDPIKQIKSKLRV 252
            SL  IKQIKSKLRV
Sbjct: 248 FSLHQIKQIKSKLRV 258

BLAST of Lcy08g006080 vs. ExPASy TrEMBL
Match: A0A6J1GJA7 (Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454845 PE=4 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 9.0e-92
Identity = 178/248 (71.77%), Postives = 205/248 (82.66%), Query Frame = 0

Query: 3   MEIKEDELLEPVSPTAQCFNSSVSSISVIVVLEFETSIDDSEITSFAMDLIPLNPQFSSI 62
           ME+KEDELL+PVSPTAQ  NSSV SIS+IVVLE E +ID+ +  SFA   I LNP F+SI
Sbjct: 1   MEMKEDELLQPVSPTAQHMNSSVQSISLIVVLELEMAIDELQFMSFAKHFIHLNPLFTSI 60

Query: 63  MVNDINGERKWKKVEVNLEQHILVPTLPSNLSLEHFDAYFNEYMINLALQELPQNKPLWE 122
           MVND+NGERKWK+VEVN+E+HI+V T PSNLSL+ FDAYFNEYM N ALQELPQNKPLWE
Sbjct: 61  MVNDVNGERKWKEVEVNIEEHIIVHTPPSNLSLQLFDAYFNEYMTNYALQELPQNKPLWE 120

Query: 123 IHIINHPTSKSIGNLILKHHHSLGDGYSMMGLLISSLTRADNPSLPLTFPSRKF-SKQPG 182
           IHIIN PTS + GNL+ K  HSLGDGYS MGLLIS +TRA NPSLPLTFPSR   ++Q G
Sbjct: 121 IHIINCPTSNAAGNLVFKIRHSLGDGYSFMGLLISGMTRAHNPSLPLTFPSRTIKAEQSG 180

Query: 183 HVLSRVSRFFLSSINSVLDFGWSIIKSTVLEDDLTPIRSGGDGVELRPIAISTMTLSLDP 242
           H+L RVS+F LSS+N+VLDFGW+I+KS+VLED LTPIRSG D VE R  AI T+T SL  
Sbjct: 181 HILGRVSQFLLSSMNTVLDFGWNIMKSSVLEDHLTPIRSGRDDVEFRTFAIRTITFSLPQ 240

Query: 243 IKQIKSKL 250
           IKQIK+KL
Sbjct: 241 IKQIKTKL 248

BLAST of Lcy08g006080 vs. ExPASy TrEMBL
Match: A0A6J1HZY6 (Diacylglycerol O-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111468185 PE=4 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 8.4e-90
Identity = 174/250 (69.60%), Postives = 203/250 (81.20%), Query Frame = 0

Query: 3   MEIKEDELLEPVSPTAQCFNSSVSSISVIVVLEFETSIDDSEITSFAMDLIPLNPQFSSI 62
           ME KEDELL+PVSP AQ  N SV SIS+I VLEF+ +ID+ +   FA DLI LNP F+S+
Sbjct: 1   METKEDELLQPVSPNAQHMNCSVQSISIIAVLEFDIAIDELKFMCFAKDLIHLNPLFTSV 60

Query: 63  MVNDINGERKWKKVEVNLEQHILVPTLPSNLSLEHFDAYFNEYMINLALQELPQNKPLWE 122
           MVND+NGERKWKKVE+ +E+HI+V T PSNLSL+ FDAYFNEYM N ALQEL QNKPLWE
Sbjct: 61  MVNDVNGERKWKKVELKIEEHIIVHTPPSNLSLQLFDAYFNEYMTNYALQELSQNKPLWE 120

Query: 123 IHIINHPTSKSIGNLILKHHHSLGDGYSMMGLLISSLTRADNPSLPLTFPSRKFS-KQPG 182
           IHIIN PTS + GNL+ K HHSLGDGYS+MG+LISS+TRA +PSLPLTFPSRK   +Q G
Sbjct: 121 IHIINCPTSNAAGNLVFKIHHSLGDGYSLMGVLISSMTRAHDPSLPLTFPSRKVKLEQSG 180

Query: 183 HVLSRVSRFFLSSINSVLDFGWSIIKSTVLEDDLTPIRSGGDGVELRPIAISTMTLSLDP 242
           H+L RV +F  SS+N+VLDFG +I+KS+VLED LTPIRSGGDGVE R  AI T T SL  
Sbjct: 181 HILGRVYQFLFSSMNTVLDFGGNIMKSSVLEDHLTPIRSGGDGVEFRTFAIRTFTFSLHQ 240

Query: 243 IKQIKSKLRV 252
           IKQIK+KL V
Sbjct: 241 IKQIKAKLGV 250

BLAST of Lcy08g006080 vs. ExPASy TrEMBL
Match: A0A6J1GNF5 (Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455510 PE=4 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 1.3e-87
Identity = 172/250 (68.80%), Postives = 203/250 (81.20%), Query Frame = 0

Query: 3   MEIKEDELLEPVSPTAQCFNSSVSSISVIVVLEFETSIDDSEITSFAMDLIPLNPQFSSI 62
           ME+KEDELL PVSP AQ  NSSV S+S+I VLEFE +ID+ +  SF   LI LNP F+SI
Sbjct: 1   MEMKEDELLRPVSPIAQHMNSSVLSVSIIGVLEFEMAIDELKFMSFPNHLIRLNPLFTSI 60

Query: 63  MVNDINGERKWKKVEVNLEQHILVPTLPSNLSLEHFDAYFNEYMINLALQELPQNKPLWE 122
           MV D+NGERKWKKVE+N+E+HI+V T PSNLSL+ FDAYFNEYM N ALQEL QNKPLWE
Sbjct: 61  MVKDVNGERKWKKVELNIEEHIIVHTPPSNLSLQLFDAYFNEYMTNYALQELSQNKPLWE 120

Query: 123 IHIINHPTSKSIGNLILKHHHSLGDGYSMMGLLISSLTRADNPSLPLTFPSRKFS-KQPG 182
           IHIIN PTS ++GNLI K HHSLGDGYS+MG+LIS++TRA NPSLPLTFP+RK   +Q G
Sbjct: 121 IHIINCPTSNAVGNLIFKIHHSLGDGYSLMGVLISTMTRARNPSLPLTFPARKVKLEQSG 180

Query: 183 HVLSRVSRFFLSSINSVLDFGWSIIKSTVLEDDLTPIRSGGDGVELRPIAISTMTLSLDP 242
           H+L  VS+F LSS+N+VLDFG +++KS+VLEDD TPIRSG D VE R   I T+T SL  
Sbjct: 181 HILGCVSQFLLSSMNTVLDFGGNMMKSSVLEDDFTPIRSGRDDVEFRTFVIRTITFSLHQ 240

Query: 243 IKQIKSKLRV 252
           IKQIK+KL V
Sbjct: 241 IKQIKTKLGV 250

BLAST of Lcy08g006080 vs. NCBI nr
Match: XP_038888791.1 (O-acyltransferase WSD1-like [Benincasa hispida])

HSP 1 Score: 363.6 bits (932), Expect = 1.9e-96
Identity = 190/257 (73.93%), Postives = 214/257 (83.27%), Query Frame = 0

Query: 1   MAMEIKEDELLEPVSPTAQCFNSSVSSISVIVVLEFETSIDDSEITSFAMDLIPLNPQFS 60
           M    +EDELLEPVSP AQCFN+SV SISVIVVLEFE  ID+++I S+A DLIPLNP FS
Sbjct: 1   MEFNEQEDELLEPVSPVAQCFNTSVLSISVIVVLEFEIPIDEAKIMSYAKDLIPLNPLFS 60

Query: 61  SIMVNDINGERKWKKVEVNLEQHILVPTLPSNLSLE--HFDAYFNEYMINLALQELPQNK 120
           SIMV DING+RKWKKVEVNLE+HILVPTLPSNLSL   ++D YF EYM +L +QELP+NK
Sbjct: 61  SIMVEDINGKRKWKKVEVNLEEHILVPTLPSNLSLVELNYDTYFKEYMTDLGVQELPKNK 120

Query: 121 PLWEIHIINHPTSKSIGNLILKHHHSLGDGYSMMGLLISSLTRADNPSLPLTFPSRKFSK 180
           PLWEIHIIN+PT  +  +LILK HHSLGDGYSMMGLLISS+ RADNPSLPLTFPSRK + 
Sbjct: 121 PLWEIHIINYPTKNAAAHLILKFHHSLGDGYSMMGLLISSMKRADNPSLPLTFPSRKINS 180

Query: 181 ----QPGHVLSRVSRFFLSSINSVLDFGWSIIKSTVLEDDLTPIRSGGDGVELRPIAIST 240
               Q  +VLS V +F LSS+NSVLDFGWSI+KS+VLEDDLTPIRSGGDGVE RPIA  T
Sbjct: 181 DHNLQSRNVLSLVPKFVLSSMNSVLDFGWSIMKSSVLEDDLTPIRSGGDGVEFRPIATWT 240

Query: 241 MTLSLDPIKQIKSKLRV 252
           +  SL  IKQIKSKLRV
Sbjct: 241 INFSLHQIKQIKSKLRV 257

BLAST of Lcy08g006080 vs. NCBI nr
Match: XP_022952996.1 (O-acyltransferase WSD1-like [Cucurbita moschata])

HSP 1 Score: 350.5 bits (898), Expect = 1.7e-92
Identity = 179/250 (71.60%), Postives = 210/250 (84.00%), Query Frame = 0

Query: 3   MEIKEDELLEPVSPTAQCFNSSVSSISVIVVLEFETSIDDSEITSFAMDLIPLNPQFSSI 62
           ME+KEDELL+PVSPTAQ  NS V SIS+I VLEFE +ID+ +  SFA DLI LNP F+SI
Sbjct: 1   MEMKEDELLQPVSPTAQSMNSFVRSISLIAVLEFEIAIDEHKFMSFAKDLIHLNPLFTSI 60

Query: 63  MVNDINGERKWKKVEVNLEQHILVPTLPSNLSLEHFDAYFNEYMINLALQELPQNKPLWE 122
           MVND+NGERKWKKVEVN+E+HI+V T PSNLSL+ FDAYFNEYM N ALQELPQNKPLWE
Sbjct: 61  MVNDVNGERKWKKVEVNIEEHIIVHTPPSNLSLQLFDAYFNEYMTNYALQELPQNKPLWE 120

Query: 123 IHIINHPTSKSIGNLILKHHHSLGDGYSMMGLLISSLTRADNPSLPLTFPSRKFS-KQPG 182
           IHIIN PT+ + GNL+ K HHSLGDGYS+MG+LISS+TRA +PSLPLTFPSRK   +Q G
Sbjct: 121 IHIINCPTNNAAGNLVFKIHHSLGDGYSLMGVLISSMTRAHDPSLPLTFPSRKVKLEQSG 180

Query: 183 HVLSRVSRFFLSSINSVLDFGWSIIKSTVLEDDLTPIRSGGDGVELRPIAISTMTLSLDP 242
           H+L RV +F LSS+N+VLDFG +++KS+VLED LTPIRSGGDGVE R  AI T+T SL  
Sbjct: 181 HILGRVYQFLLSSMNTVLDFGGNMMKSSVLEDHLTPIRSGGDGVEFRTFAIRTITFSLHH 240

Query: 243 IKQIKSKLRV 252
           IK+IK+KL V
Sbjct: 241 IKKIKAKLGV 250

BLAST of Lcy08g006080 vs. NCBI nr
Match: KAE8645785.1 (hypothetical protein Csa_017199 [Cucumis sativus])

HSP 1 Score: 349.4 bits (895), Expect = 3.8e-92
Identity = 184/255 (72.16%), Postives = 208/255 (81.57%), Query Frame = 0

Query: 6   KEDELLEPVSPTAQCFNSSVSSISVIVVLEFETSIDDSEITSFAMDLIPLNPQFSSIMVN 65
           +E+ELLEPVSPTAQCFNSS  SISVI VLEFE  ID+ +I  +A DLIPLNP FSSIMV+
Sbjct: 8   EEEELLEPVSPTAQCFNSSALSISVIAVLEFEIPIDEVQIMCYAKDLIPLNPLFSSIMVD 67

Query: 66  DINGERKWKKVEVNLEQHILVPTLPSNLSLE---HFDAYFNEYMINLALQELPQNKPLWE 125
           DINGERKWKKVEVNLE+HILVPT PSNLS+    ++DAYFN+Y+ NL  QEL +NKPLWE
Sbjct: 68  DINGERKWKKVEVNLEEHILVPTFPSNLSVVDDINYDAYFNKYITNLGAQELAKNKPLWE 127

Query: 126 IHIINHPTSKSIGNLILKHHHSLGDGYSMMGLLISSLTRADNPSLPLTFPSRKF------ 185
           IHII +PTS +  NLILK HHSLGDGYS+MGLLISS+ RADNPSLPLTFPSRK       
Sbjct: 128 IHIIKYPTSNAAANLILKFHHSLGDGYSVMGLLISSMKRADNPSLPLTFPSRKINSDDHN 187

Query: 186 SKQPGHVLSRVSRFFLSSINSVLDFGWSIIKSTVLEDDLTPIRSGGDGVELRPIAISTMT 245
           +K   HVL+ V  F    +NSVLDFGWSIIKS+VLEDDLTPIRSGGDGVE RP+AI T++
Sbjct: 188 NKNLRHVLNYVPSF----MNSVLDFGWSIIKSSVLEDDLTPIRSGGDGVEFRPVAICTIS 247

Query: 246 LSLDPIKQIKSKLRV 252
            SL  IKQIKSKLRV
Sbjct: 248 FSLHQIKQIKSKLRV 258

BLAST of Lcy08g006080 vs. NCBI nr
Match: XP_022952086.1 (O-acyltransferase WSD1-like [Cucurbita moschata])

HSP 1 Score: 347.1 bits (889), Expect = 1.9e-91
Identity = 178/248 (71.77%), Postives = 205/248 (82.66%), Query Frame = 0

Query: 3   MEIKEDELLEPVSPTAQCFNSSVSSISVIVVLEFETSIDDSEITSFAMDLIPLNPQFSSI 62
           ME+KEDELL+PVSPTAQ  NSSV SIS+IVVLE E +ID+ +  SFA   I LNP F+SI
Sbjct: 1   MEMKEDELLQPVSPTAQHMNSSVQSISLIVVLELEMAIDELQFMSFAKHFIHLNPLFTSI 60

Query: 63  MVNDINGERKWKKVEVNLEQHILVPTLPSNLSLEHFDAYFNEYMINLALQELPQNKPLWE 122
           MVND+NGERKWK+VEVN+E+HI+V T PSNLSL+ FDAYFNEYM N ALQELPQNKPLWE
Sbjct: 61  MVNDVNGERKWKEVEVNIEEHIIVHTPPSNLSLQLFDAYFNEYMTNYALQELPQNKPLWE 120

Query: 123 IHIINHPTSKSIGNLILKHHHSLGDGYSMMGLLISSLTRADNPSLPLTFPSRKF-SKQPG 182
           IHIIN PTS + GNL+ K  HSLGDGYS MGLLIS +TRA NPSLPLTFPSR   ++Q G
Sbjct: 121 IHIINCPTSNAAGNLVFKIRHSLGDGYSFMGLLISGMTRAHNPSLPLTFPSRTIKAEQSG 180

Query: 183 HVLSRVSRFFLSSINSVLDFGWSIIKSTVLEDDLTPIRSGGDGVELRPIAISTMTLSLDP 242
           H+L RVS+F LSS+N+VLDFGW+I+KS+VLED LTPIRSG D VE R  AI T+T SL  
Sbjct: 181 HILGRVSQFLLSSMNTVLDFGWNIMKSSVLEDHLTPIRSGRDDVEFRTFAIRTITFSLPQ 240

Query: 243 IKQIKSKL 250
           IKQIK+KL
Sbjct: 241 IKQIKTKL 248

BLAST of Lcy08g006080 vs. NCBI nr
Match: KAG6572370.1 (O-acyltransferase WSD1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 345.9 bits (886), Expect = 4.2e-91
Identity = 177/250 (70.80%), Postives = 209/250 (83.60%), Query Frame = 0

Query: 3   MEIKEDELLEPVSPTAQCFNSSVSSISVIVVLEFETSIDDSEITSFAMDLIPLNPQFSSI 62
           ME+KE+ELL+PVSPTAQ  NS V SIS+I VLEFE +ID+ +  SFA DLI LNP F+SI
Sbjct: 1   MEMKEEELLQPVSPTAQSMNSFVRSISLIAVLEFEIAIDEHKFMSFAKDLIHLNPLFTSI 60

Query: 63  MVNDINGERKWKKVEVNLEQHILVPTLPSNLSLEHFDAYFNEYMINLALQELPQNKPLWE 122
           MVND+NGERKWKKVEVN+E+HI+V T PSNLSL+ FDAYFNEYM N ALQEL QNKPLWE
Sbjct: 61  MVNDVNGERKWKKVEVNIEEHIIVHTPPSNLSLQLFDAYFNEYMTNYALQELSQNKPLWE 120

Query: 123 IHIINHPTSKSIGNLILKHHHSLGDGYSMMGLLISSLTRADNPSLPLTFPSRKFS-KQPG 182
           IHIIN PT+ + GNL+ K HHSLGDGYS+MG+LISS+TRA +PSLPLTFPSRK   +Q G
Sbjct: 121 IHIINCPTNNAAGNLVFKIHHSLGDGYSLMGVLISSMTRAHDPSLPLTFPSRKVKLEQSG 180

Query: 183 HVLSRVSRFFLSSINSVLDFGWSIIKSTVLEDDLTPIRSGGDGVELRPIAISTMTLSLDP 242
           H+L RV +F LSS+N+VLDFG +++KS+VLED LTPIRSGGDGVE R  AI T+T SL  
Sbjct: 181 HILGRVYQFLLSSMNTVLDFGGNMMKSSVLEDHLTPIRSGGDGVEFRTFAIRTITFSLHH 240

Query: 243 IKQIKSKLRV 252
           IK+IK+KL V
Sbjct: 241 IKKIKAKLGV 250

BLAST of Lcy08g006080 vs. TAIR 10
Match: AT5G12420.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 117.9 bits (294), Expect = 1.7e-26
Identity = 78/251 (31.08%), Postives = 133/251 (52.99%), Query Frame = 0

Query: 12  EPVSPTAQCFNSSVSSISVIVVLEFETSIDDSEITSFAMDLIPLNPQFSSIMVNDINGER 71
           EPVSP A+ F S    +  + ++ F+T I+   +       +  +P+FSSI+ +  NG  
Sbjct: 7   EPVSPMARVFQSPGIDLCAVTIMGFKTKINPDVVLDALKQNVSKHPRFSSILSD--NG-A 66

Query: 72  KWKKVEVNLEQHILVPTLPSNLSLEHFDAYFNEYMINLALQELPQNKPLWEIHIINHPTS 131
           KW + EVN+E H++VP + +    E   ++ ++YM  L +  L +++PLW+IHI+N  TS
Sbjct: 67  KWIETEVNVEDHVIVPYIDAEEIGEGGQSFIDDYMSRLTMIPLDRSRPLWDIHILNVKTS 126

Query: 132 KSIGNLILKHHHSLGDGYSMMGLLISSLTRADNPSL-PLTFPSRKFSKQPGHVLSRVSRF 191
           ++     ++ HHSLGDG S++ L+++   +  +P +     PS K      H L     F
Sbjct: 127 EAEAVGFIRSHHSLGDGMSLISLMLACTHKTSDPDMFSNAIPSMKRRATMSHSLKTKGWF 186

Query: 192 FLS--SINSVLDFGWS------IIKSTV--LEDDLTPIRSGGDGVELRPIAISTMTLSLD 251
             S  +I S +   W+      ++ +TV  L+D  TP+++G D V   P       +SLD
Sbjct: 187 LRSIFTIGSTMRLLWNTTIDMLLLLATVLFLKDTKTPLKAGAD-VRSNPKRFYHRIISLD 246

BLAST of Lcy08g006080 vs. TAIR 10
Match: AT3G49200.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 114.0 bits (284), Expect = 2.5e-25
Identity = 78/269 (29.00%), Postives = 140/269 (52.04%), Query Frame = 0

Query: 4   EIKEDELLEPVSPTAQCFNSSVSSISVIVVLEFETSIDDSEITSFAMDLIPLNPQFSSIM 63
           E++E+E  +P+SP A+ F++   +  +I V+  +  I+   I       +  +P+FSS +
Sbjct: 20  EVEEEE--QPLSPAARLFHAPEFNCYIISVVGLKNKIEPDMIIEGIKQTLMRHPRFSSKL 79

Query: 64  VNDING---ERKWKKVEVNLEQHILVPTLPS-NLSLEHFDAYFNEYMINLALQELPQNKP 123
           VN+ N    E+KW +  V +E H+++P + + ++   + D +   Y+ +L    L  +KP
Sbjct: 80  VNNCNNNRQEQKWVRTNVVVEDHVIIPKIQTQHIENANADVFLESYVSDLTTIPLDTSKP 139

Query: 124 LWEIHIINHPTSKSIGNLILKHHHSLGDGYSMMGLLISSLTRADNPSLPLTFPSRKFSKQ 183
           LWE+H+++  TS +    +L+ HHSLGDG SMM L+++   +  NP+     PS  +  +
Sbjct: 140 LWEVHLLDLKTSDAENVAVLRIHHSLGDGMSMMSLVLACTRKTSNPN---ELPSLPYQNR 199

Query: 184 PGHVLSRV-------SRFF----------LSSINSVLDFGWSIIKSTVLEDDLTPIRSGG 243
           P    S +       SRFF          L  +N+V D    I  +  L+D  TPI+   
Sbjct: 200 PSSGSSSLKTSSRCYSRFFWLVMVLWSAALLVLNTVCDALEFIATALFLKDTETPIKGDF 259

Query: 244 DGVELRPIAISTMTLSLDPIKQIKSKLRV 252
              + + + +   T+SLD IK IK+ +++
Sbjct: 260 KLSKGKRMCMVHRTVSLDDIKLIKNAMKM 283

BLAST of Lcy08g006080 vs. TAIR 10
Match: AT2G38995.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 112.8 bits (281), Expect = 5.5e-25
Identity = 77/253 (30.43%), Postives = 128/253 (50.59%), Query Frame = 0

Query: 1   MAMEIKEDELLEPVSPTAQCFNSSVSSISVIVVLEFETSIDDSEITSFAMDLIPLNPQFS 60
           MA E +E   +EP+SP +Q F S      +I  L F+T  + S I     +     P+FS
Sbjct: 10  MAKEEQETAAIEPLSPVSQLFVSPSLYCFIIFTLGFQTRCNPSTIVEGVKNTWIKLPRFS 69

Query: 61  SIMVNDINGERKWKKVEVNLEQHILVPTLPSNLSLEHFDAYFNEYMINLALQELPQNKPL 120
           S +    NG+  W  V V +E H++VP L  + ++E+ D +  +Y   LA   +  ++PL
Sbjct: 70  SKVEIKKNGKASWVPVSVRVEDHVVVPDLDYS-NIENPDQFIEDYTSKLANTPMDMSRPL 129

Query: 121 WEIHIINHPTSKSIGNLILKHHHSLGDGYSMMGLLISSLTRADNP-SLPLTFPSRKFSKQ 180
           WE+H++N  TS +    I K HHSLGDG S++ LL++S  +  +P +LP T  +RK +  
Sbjct: 130 WELHLLNIKTSNAESLAIGKFHHSLGDGMSLISLLLASSRKTSDPDALPTTAATRKHASS 189

Query: 181 PGHVLSRVSRFFLS---SINSVLDFGWSIIKSTVLEDDLTPIRSGGDGVELRPIAISTMT 240
                  V RF+        +V++    ++    + D  TP+  G  G  +R   +    
Sbjct: 190 NKKSWWLVGRFWFMIRIIFTTVVELFKYLLTLCFMRDTKTPL-MGKTGDAIRSRKVIHRI 249

Query: 241 LSLDPIKQIKSKL 250
           +S D +K +K+ +
Sbjct: 250 VSFDDVKLVKNNM 260

BLAST of Lcy08g006080 vs. TAIR 10
Match: AT2G38995.2 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 112.8 bits (281), Expect = 5.5e-25
Identity = 77/253 (30.43%), Postives = 128/253 (50.59%), Query Frame = 0

Query: 1   MAMEIKEDELLEPVSPTAQCFNSSVSSISVIVVLEFETSIDDSEITSFAMDLIPLNPQFS 60
           MA E +E   +EP+SP +Q F S      +I  L F+T  + S I     +     P+FS
Sbjct: 10  MAKEEQETAAIEPLSPVSQLFVSPSLYCFIIFTLGFQTRCNPSTIVEGVKNTWIKLPRFS 69

Query: 61  SIMVNDINGERKWKKVEVNLEQHILVPTLPSNLSLEHFDAYFNEYMINLALQELPQNKPL 120
           S +    NG+  W  V V +E H++VP L  + ++E+ D +  +Y   LA   +  ++PL
Sbjct: 70  SKVEIKKNGKASWVPVSVRVEDHVVVPDLDYS-NIENPDQFIEDYTSKLANTPMDMSRPL 129

Query: 121 WEIHIINHPTSKSIGNLILKHHHSLGDGYSMMGLLISSLTRADNP-SLPLTFPSRKFSKQ 180
           WE+H++N  TS +    I K HHSLGDG S++ LL++S  +  +P +LP T  +RK +  
Sbjct: 130 WELHLLNIKTSNAESLAIGKFHHSLGDGMSLISLLLASSRKTSDPDALPTTAATRKHASS 189

Query: 181 PGHVLSRVSRFFLS---SINSVLDFGWSIIKSTVLEDDLTPIRSGGDGVELRPIAISTMT 240
                  V RF+        +V++    ++    + D  TP+  G  G  +R   +    
Sbjct: 190 NKKSWWLVGRFWFMIRIIFTTVVELFKYLLTLCFMRDTKTPL-MGKTGDAIRSRKVIHRI 249

Query: 241 LSLDPIKQIKSKL 250
           +S D +K +K+ +
Sbjct: 250 VSFDDVKLVKNNM 260

BLAST of Lcy08g006080 vs. TAIR 10
Match: AT3G49190.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 110.9 bits (276), Expect = 2.1e-24
Identity = 73/262 (27.86%), Postives = 139/262 (53.05%), Query Frame = 0

Query: 6   KEDELLEPVSPTAQCFNSSVSSISVIVVLEFETSIDDSEITSFAMDLIPLNPQFSSIMVN 65
           KE+E  +P+SP A+ F++   + ++I V+ F++ +D         +    +P+FSS +V 
Sbjct: 14  KEEE--QPLSPAARLFHAPEFNCNIISVIGFKSKLDPCVFIRGFKESFIRHPRFSSKLVT 73

Query: 66  DINGE-RKWKKVEVNLEQHILVPTL-PSNLSLEHFDAYFNEYMINLALQELPQNKPLWEI 125
           D NG+ ++W +  V +E H +VP + P N  +E+ +A+  +Y+ +L    L  ++PLWE+
Sbjct: 74  DENGQNQRWVRTNVVVEDHFIVPKIKPQN--IENSNAFLEDYVSDLMKIPLDTSRPLWEL 133

Query: 126 HIINHPTSKSIGNLILKHHHSLGDGYSMMGLLISSLTRADNPSLPLTFPSRKFSKQPGHV 185
           H+++  TS +    +LK HHS+GDG S+M L+++ + +  NP    + P +  S     +
Sbjct: 134 HLLDLKTSDAENVAVLKIHHSVGDGMSIMSLVLACMRKTSNPDELPSLPYQYRSSSRSSL 193

Query: 186 LSRVSR--------------FFLSSINSVLDFGWSIIKSTVLEDDLTPIRSGGDGVELRP 245
           L+  SR                +  +N+V D    I+ +  ++D  TPI+      + + 
Sbjct: 194 LTTGSRSDSRLLWLVKVIWTAVILGLNTVCDALEFIVTTLFVKDTETPIKGDFLSTKSKQ 253

Query: 246 IAISTMTLSLDPIKQIKSKLRV 252
           + +   T+SLD IK  K+ + +
Sbjct: 254 LRLVHRTVSLDDIKLTKNAMNM 271

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94CK02.4e-2531.08Wax ester synthase/diacylglycerol acyltransferase 7 OS=Arabidopsis thaliana OX=3... [more]
Q9M3B23.5e-2429.00Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3... [more]
F4IU147.8e-2430.43Wax ester synthase/diacylglycerol acyltransferase 3 OS=Arabidopsis thaliana OX=3... [more]
Q9M3B32.9e-2327.86Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3... [more]
Q9C7H41.1e-2229.18Wax ester synthase/diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1GLS68.2e-9371.60Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455511 P... [more]
A0A0A0K7491.8e-9272.16Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G051310 PE=4... [more]
A0A6J1GJA79.0e-9271.77Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454845 P... [more]
A0A6J1HZY68.4e-9069.60Diacylglycerol O-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111468185 PE=... [more]
A0A6J1GNF51.3e-8768.80Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455510 P... [more]
Match NameE-valueIdentityDescription
XP_038888791.11.9e-9673.93O-acyltransferase WSD1-like [Benincasa hispida][more]
XP_022952996.11.7e-9271.60O-acyltransferase WSD1-like [Cucurbita moschata][more]
KAE8645785.13.8e-9272.16hypothetical protein Csa_017199 [Cucumis sativus][more]
XP_022952086.11.9e-9171.77O-acyltransferase WSD1-like [Cucurbita moschata][more]
KAG6572370.14.2e-9170.80O-acyltransferase WSD1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT5G12420.11.7e-2631.08O-acyltransferase (WSD1-like) family protein [more]
AT3G49200.12.5e-2529.00O-acyltransferase (WSD1-like) family protein [more]
AT2G38995.15.5e-2530.43O-acyltransferase (WSD1-like) family protein [more]
AT2G38995.25.5e-2530.43O-acyltransferase (WSD1-like) family protein [more]
AT3G49190.12.1e-2427.86O-acyltransferase (WSD1-like) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004255O-acyltransferase, WSD1, N-terminalPFAMPF03007WES_acyltransfcoord: 72..187
e-value: 2.6E-7
score: 30.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 311..336
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 311..356
NoneNo IPR availablePANTHERPTHR31650:SF34O-ACYLTRANSFERASE WSD1-LIKEcoord: 8..251
IPR045034O-acyltransferase WSD1-likePANTHERPTHR31650O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEINcoord: 8..251

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy08g006080.1Lcy08g006080.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019432 triglyceride biosynthetic process
biological_process GO:0045017 glycerolipid biosynthetic process
cellular_component GO:0005886 plasma membrane
molecular_function GO:0004144 diacylglycerol O-acyltransferase activity
molecular_function GO:0047196 long-chain-alcohol O-fatty-acyltransferase activity
molecular_function GO:0008374 O-acyltransferase activity