Lcy08g003900 (gene) Sponge gourd (P93075) v1

Overview
NameLcy08g003900
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionHomoserine kinase
LocationChr08: 5159604 .. 5161467 (-)
RNA-Seq ExpressionLcy08g003900
SyntenyLcy08g003900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAATGGCCACAATGTGAAAAGCCAAATGACGAAATTGCCTATCATTTTAAGCCCTTCCTCAACCTCGCTCTTCGAAAAAGCCGCCTCAAATGGTAATTTGGTTACCCATATATAAACACGCAGGTTCTTCGTCTTCATTTCATCTGAGGTTCATTTGACTCACCGCCGTGACTGCGCTGAGCCTCTGAGGCTAAAAATCTTCATCTCTGAGAAATTTTCAGGTTCCGATCTCAATCTCATTCCTCTTTCACTTGCTTCCATGGCGATGATCTCCTATCAGCCTCCATTTAGGTCGTTGGCGGTTCCTCCAGTTTCAATATCTAACCCAAAATCTGTCATCTTCAGGTGCAATCTGTCTCTTCCATCCAGAACCGCCGTCACTACCGTCGAACCTGAACCAGTATTCTCGTCCGTCAAGGCGTTCGCACCTGCAACGGTCGCCAATTTGGGCCCTGGCTTCGATTTCCTTGGCTGCGCCGTCGATGGCTTGGGCGATTCCGTCTCTCTCAGCGTCGATGCGAATGTTCATCCAGGTGAGGTCGCAATCACCAATATTACAGGCGATAACTCCAATAAACTCAGTAAAAATCCTCTGTATAATTGCGCTGGTATTGCCGCCATTGAGGTGATGAAAATGTTAGGGATCCGATCTGTCGGTCTCTCTCTTTCGCTCGAGAAGGGCTTGCCGTTGGGGAGTGGATTGGGATCGAGTGCAGCGAGTGCCGCAGCTGCGGCGGTTGCTGTTAATGGATTGTTTGGCGGAAAATTAGGAGTCGATGAATTGGTTCTCGCGGGGCTGAAGTCGGAAGAGAAGGTTTCTGGATACCATGCGGACAATGTCGCCCCTGCGATCATGGGGGGTTTCATTCTGATCCGAAATTACGAACCCTTGGAATTGATCCGCCTGAAATTCCCCGTCGAGAAGGAGCTGTTCTTCGTATTGGTGAGCCCGGAATTCGAGGCTCCGACGAAGAAAATGCGGGCTGCGCTGCCGGCGGAAGTTGGGATGCCGCACCACGTATGGAATTGCAGCCAAGCAGGGGCGTTGGTGGCGGCTGTGCTGCAGGGCGACTTGATGGGGTTGGGCAAAGCATTGTCGTCGGACAAAATTGTAGAGCCAAGGCGGGCGCCACTGATTCCAGGGATGGATGGGGTCAAGAAGGCGGCCATTGCAGCCGGGGCATTCGGGTGTACGATAAGCGGGGCAGGGCCGACCGCAGTGGCGGTGGTCGACAATGAGGAGAAGGGGAAGGAGATTGGTGAGAGGATGGCTATGGCGTTTTTGAAGGAAGGGAATTTGAAAGCTGTGGCATCTGTACAGAGGCTTGATCGAGTTGGTGCCAGGCTTATAGGATCCACTCCAAGATAGTGTTTTATGAGAGTATGTGTTTCTGATGTTTTGATGCTCTGCTTCTAATGCAAGTGCTTGCTGTGGCTTCATCTGAGAAATGGTTAATAAATTCGATTCCGCCATGAAAATGAACCTCTGAAACCTATTTGTTTATGCGGAATTATCTGGTGCATTTTTGTTGCAGAAGCTTGGTTTTTATCAGTTGAACTGTAGCTTGTGAAATTGAAATCTGGATAGTCTGAGTTCAATAGGCTCATCAGAGCTGATTGGTTCTTGATCTAATCCTCGGCCTGAAAATGAAGAATCTTGGTAAGGAACAATAATCTCGTATAAAAAGCGAGCTTTGGCTAATTAGTTAACTGTTCTAACTTAATAGCGCTGGATATCAATTTTAATCTTATGTGCTGTTTCTTTGGTAAAGGTCAGCAATATAAGTACCTAACTAAGGATGTCCATTTGTCCCCATCCCCACGGGACCCGTCTCTAATGGGGTGGAGAATCCCCATTTA

mRNA sequence

AAAAAAATGGCCACAATGTGAAAAGCCAAATGACGAAATTGCCTATCATTTTAAGCCCTTCCTCAACCTCGCTCTTCGAAAAAGCCGCCTCAAATGGTAATTTGGTTACCCATATATAAACACGCAGGTTCTTCGTCTTCATTTCATCTGAGGTTCATTTGACTCACCGCCGTGACTGCGCTGAGCCTCTGAGGCTAAAAATCTTCATCTCTGAGAAATTTTCAGGTTCCGATCTCAATCTCATTCCTCTTTCACTTGCTTCCATGGCGATGATCTCCTATCAGCCTCCATTTAGGTCGTTGGCGGTTCCTCCAGTTTCAATATCTAACCCAAAATCTGTCATCTTCAGGTGCAATCTGTCTCTTCCATCCAGAACCGCCGTCACTACCGTCGAACCTGAACCAGTATTCTCGTCCGTCAAGGCGTTCGCACCTGCAACGGTCGCCAATTTGGGCCCTGGCTTCGATTTCCTTGGCTGCGCCGTCGATGGCTTGGGCGATTCCGTCTCTCTCAGCGTCGATGCGAATGTTCATCCAGGTGAGGTCGCAATCACCAATATTACAGGCGATAACTCCAATAAACTCAGTAAAAATCCTCTGTATAATTGCGCTGGTATTGCCGCCATTGAGGTGATGAAAATGTTAGGGATCCGATCTGTCGGTCTCTCTCTTTCGCTCGAGAAGGGCTTGCCGTTGGGGAGTGGATTGGGATCGAGTGCAGCGAGTGCCGCAGCTGCGGCGGTTGCTGTTAATGGATTGTTTGGCGGAAAATTAGGAGTCGATGAATTGGTTCTCGCGGGGCTGAAGTCGGAAGAGAAGGTTTCTGGATACCATGCGGACAATGTCGCCCCTGCGATCATGGGGGGTTTCATTCTGATCCGAAATTACGAACCCTTGGAATTGATCCGCCTGAAATTCCCCGTCGAGAAGGAGCTGTTCTTCGTATTGGTGAGCCCGGAATTCGAGGCTCCGACGAAGAAAATGCGGGCTGCGCTGCCGGCGGAAGTTGGGATGCCGCACCACGTATGGAATTGCAGCCAAGCAGGGGCGTTGGTGGCGGCTGTGCTGCAGGGCGACTTGATGGGGTTGGGCAAAGCATTGTCGTCGGACAAAATTGTAGAGCCAAGGCGGGCGCCACTGATTCCAGGGATGGATGGGGTCAAGAAGGCGGCCATTGCAGCCGGGGCATTCGGGTGTACGATAAGCGGGGCAGGGCCGACCGCAGTGGCGGTGGTCGACAATGAGGAGAAGGGGAAGGAGATTGGTGAGAGGATGGCTATGGCGTTTTTGAAGGAAGGGAATTTGAAAGCTGTGGCATCTGTACAGAGGCTTGATCGAGTTGGTGCCAGGCTTATAGGATCCACTCCAAGATAGTGTTTTATGAGAGTATGTGTTTCTGATGTTTTGATGCTCTGCTTCTAATGCAAGTGCTTGCTGTGGCTTCATCTGAGAAATGGTTAATAAATTCGATTCCGCCATGAAAATGAACCTCTGAAACCTATTTGTTTATGCGGAATTATCTGGTGCATTTTTGTTGCAGAAGCTTGGTTTTTATCAGTTGAACTGTAGCTTGTGAAATTGAAATCTGGATAGTCTGAGTTCAATAGGCTCATCAGAGCTGATTGGTTCTTGATCTAATCCTCGGCCTGAAAATGAAGAATCTTGGTAAGGAACAATAATCTCGTATAAAAAGCGAGCTTTGGCTAATTAGTTAACTGTTCTAACTTAATAGCGCTGGATATCAATTTTAATCTTATGTGCTGTTTCTTTGGTAAAGGTCAGCAATATAAGTACCTAACTAAGGATGTCCATTTGTCCCCATCCCCACGGGACCCGTCTCTAATGGGGTGGAGAATCCCCATTTA

Coding sequence (CDS)

ATGGCGATGATCTCCTATCAGCCTCCATTTAGGTCGTTGGCGGTTCCTCCAGTTTCAATATCTAACCCAAAATCTGTCATCTTCAGGTGCAATCTGTCTCTTCCATCCAGAACCGCCGTCACTACCGTCGAACCTGAACCAGTATTCTCGTCCGTCAAGGCGTTCGCACCTGCAACGGTCGCCAATTTGGGCCCTGGCTTCGATTTCCTTGGCTGCGCCGTCGATGGCTTGGGCGATTCCGTCTCTCTCAGCGTCGATGCGAATGTTCATCCAGGTGAGGTCGCAATCACCAATATTACAGGCGATAACTCCAATAAACTCAGTAAAAATCCTCTGTATAATTGCGCTGGTATTGCCGCCATTGAGGTGATGAAAATGTTAGGGATCCGATCTGTCGGTCTCTCTCTTTCGCTCGAGAAGGGCTTGCCGTTGGGGAGTGGATTGGGATCGAGTGCAGCGAGTGCCGCAGCTGCGGCGGTTGCTGTTAATGGATTGTTTGGCGGAAAATTAGGAGTCGATGAATTGGTTCTCGCGGGGCTGAAGTCGGAAGAGAAGGTTTCTGGATACCATGCGGACAATGTCGCCCCTGCGATCATGGGGGGTTTCATTCTGATCCGAAATTACGAACCCTTGGAATTGATCCGCCTGAAATTCCCCGTCGAGAAGGAGCTGTTCTTCGTATTGGTGAGCCCGGAATTCGAGGCTCCGACGAAGAAAATGCGGGCTGCGCTGCCGGCGGAAGTTGGGATGCCGCACCACGTATGGAATTGCAGCCAAGCAGGGGCGTTGGTGGCGGCTGTGCTGCAGGGCGACTTGATGGGGTTGGGCAAAGCATTGTCGTCGGACAAAATTGTAGAGCCAAGGCGGGCGCCACTGATTCCAGGGATGGATGGGGTCAAGAAGGCGGCCATTGCAGCCGGGGCATTCGGGTGTACGATAAGCGGGGCAGGGCCGACCGCAGTGGCGGTGGTCGACAATGAGGAGAAGGGGAAGGAGATTGGTGAGAGGATGGCTATGGCGTTTTTGAAGGAAGGGAATTTGAAAGCTGTGGCATCTGTACAGAGGCTTGATCGAGTTGGTGCCAGGCTTATAGGATCCACTCCAAGATAG

Protein sequence

MAMISYQPPFRSLAVPPVSISNPKSVIFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVANLGPGFDFLGCAVDGLGDSVSLSVDANVHPGEVAITNITGDNSNKLSKNPLYNCAGIAAIEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAVAVNGLFGGKLGVDELVLAGLKSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAALPAEVGMPHHVWNCSQAGALVAAVLQGDLMGLGKALSSDKIVEPRRAPLIPGMDGVKKAAIAAGAFGCTISGAGPTAVAVVDNEEKGKEIGERMAMAFLKEGNLKAVASVQRLDRVGARLIGSTPR
Homology
BLAST of Lcy08g003900 vs. ExPASy Swiss-Prot
Match: Q8L7R2 (Homoserine kinase OS=Arabidopsis thaliana OX=3702 GN=HSK PE=1 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 2.4e-137
Identity = 269/379 (70.98%), Postives = 305/379 (80.47%), Query Frame = 0

Query: 1   MAMISYQPPFRSLAVPPVSISNPKS----------VIFRCNLSLPSRTAVTTVEPEPVFS 60
           MA + +Q P +     P+S   PKS           +FRC  S+ +  A   VEPEPVF 
Sbjct: 1   MASLCFQSPSK-----PISYFQPKSNPSPPLFAKVSVFRCRASVQTLVA---VEPEPVFV 60

Query: 61  SVKAFAPATVANLGPGFDFLGCAVDGLGDSVSLSVDANVHPGEVAITNITGDNSNKLSKN 120
           SVK FAPATVANLGPGFDFLGCAVDGLGD V+L VD +V  GEV+I+ ITG  + KLS N
Sbjct: 61  SVKTFAPATVANLGPGFDFLGCAVDGLGDHVTLRVDPSVRAGEVSISEITG-TTTKLSTN 120

Query: 121 PLYNCAGIAAIEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAVAVNGLFGGKL 180
           PL NCAGIAAI  MKMLGIRSVGLSL L KGLPLGSGLGSSAASAAAAAVAVN +FG KL
Sbjct: 121 PLRNCAGIAAIATMKMLGIRSVGLSLDLHKGLPLGSGLGSSAASAAAAAVAVNEIFGRKL 180

Query: 181 GVDELVLAGLKSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVS 240
           G D+LVLAGL+SE KVSGYHADN+APAIMGGF+LIRNYEPL+L  L+FP +K+LFFVLVS
Sbjct: 181 GSDQLVLAGLESEAKVSGYHADNIAPAIMGGFVLIRNYEPLDLKPLRFPSDKDLFFVLVS 240

Query: 241 PEFEAPTKKMRAALPAEVGMPHHVWNCSQAGALVAAVLQGDLMGLGKALSSDKIVEPRRA 300
           P+FEAPTKKMRAALP E+ M HHVWN SQA ALVAAVL+GD + LGKALSSDKIVEP RA
Sbjct: 241 PDFEAPTKKMRAALPTEIPMVHHVWNSSQAAALVAAVLEGDAVMLGKALSSDKIVEPTRA 300

Query: 301 PLIPGMDGVKKAAIAAGAFGCTISGAGPTAVAVVDNEEKGKEIGERMAMAFLKEGNLKAV 360
           PLIPGM+ VKKAA+ AGAFGCTISGAGPTAVAV+D+EEKG+ IGE+M  AF K G+LK+V
Sbjct: 301 PLIPGMEAVKKAALEAGAFGCTISGAGPTAVAVIDSEEKGQVIGEKMVEAFWKVGHLKSV 360

Query: 361 ASVQRLDRVGARLIGSTPR 370
           ASV++LD VGARL+ S  R
Sbjct: 361 ASVKKLDNVGARLVNSVSR 370

BLAST of Lcy08g003900 vs. ExPASy Swiss-Prot
Match: A4SGN0 (Homoserine kinase OS=Chlorobium phaeovibrioides (strain DSM 265 / 1930) OX=290318 GN=thrB PE=3 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 1.8e-47
Identity = 136/322 (42.24%), Postives = 185/322 (57.45%), Query Frame = 0

Query: 51  SVKAFAPATVANLGPGFDFLGCAVDGLGDSVSLSVDANVHPG-EVAITNITGDNSNKLSK 110
           SV+ FA ATV N+  GFD LG A+   GD V L+     H G  V I++I+GD   KL  
Sbjct: 3   SVRGFASATVGNVACGFDILGFAITEPGDEVILTRSERKHTGCPVTISSISGD-GGKLPL 62

Query: 111 NPLYNCAGIAAIEVMKML----GIRSVG-LSLSLEKGLPLGSGLGSSAASAAAAAVAVNG 170
           +P  N +    ++ ++ +    G    G +SL L+K LPL SG+GSSAASAAAA  A N 
Sbjct: 63  DPRKNTSSFVVLKFLEHIRTHKGQDCTGHISLELKKHLPLSSGMGSSAASAAAALAAANE 122

Query: 171 LFGGKLGVDELVLAGLKSEEKVSG-YHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKE 230
           L G      ELV   ++ E    G  HADN APAI+G F+LIR+Y PL+LI +  P  ++
Sbjct: 123 LLGRPCTKMELVHFAIEGERVACGSAHADNAAPAILGNFVLIRSYTPLDLIVI--PPPEK 182

Query: 231 LFFVLVSPEFEAPTKKMRAALPAEVGMPHHVWNCSQAGALVAAVLQGDLMGLGKALSSDK 290
           LF  LV P  E  T   R+ LP ++ +          GALVA +L  D   +G++L  D 
Sbjct: 183 LFCSLVHPHTELRTSFARSVLPTDITLKDATRQWGNVGALVAGLLTSDYALVGRSL-VDV 242

Query: 291 IVEPRRAPLIPGMDGVKKAAIAAGAFGCTISGAGPTAVAVVDNEEKGKEIGERMAMAFL- 350
           + EP+RAPLIPG   VK+AA+ AGA GC+I+G+GP+  A  D+ E     GE M  AFL 
Sbjct: 243 VAEPKRAPLIPGFAEVKEAALNAGALGCSIAGSGPSIFAFSDSAETAHAAGEAMQKAFLA 302

Query: 351 KEGNLKAVASVQRLDRVGARLI 365
            + +LK+   V  + R GAR++
Sbjct: 303 SKKSLKSDMWVSPICREGARIL 320

BLAST of Lcy08g003900 vs. ExPASy Swiss-Prot
Match: B3QWX6 (Homoserine kinase OS=Chloroherpeton thalassium (strain ATCC 35110 / GB-78) OX=517418 GN=thrB PE=3 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 2.3e-47
Identity = 134/320 (41.88%), Postives = 181/320 (56.56%), Query Frame = 0

Query: 52  VKAFAPATVANLGPGFDFLGCAVDGLGDSVSLS-VDANVHPGEVAITNITGDNSNKLSKN 111
           V  FAPA+V N+  GFD LG A+   GD V++S  D    P +V I+ I GD    L  +
Sbjct: 4   VTGFAPASVGNVACGFDVLGFALTEPGDEVTVSFTDDENSPAQVIISKIIGD-GGALPTD 63

Query: 112 PLYNCAGIAAIEVMKML----GIRSVG-LSLSLEKGLPLGSGLGSSAASAAAAAVAVNGL 171
           P  N A I  I+ ++ L    GI   G  S+ L+K LPL SG+GSSA+SAAAA +A N L
Sbjct: 64  PYKNTASIVVIKFLEFLKTHKGIEQSGTFSIELKKNLPLSSGMGSSASSAAAALIAANSL 123

Query: 172 FGGKLGVDELVLAGLKSEEKVSG-YHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKEL 231
            G      ELV   ++ E    G  HADN APA++G FIL R+Y+PL+LI +  P   EL
Sbjct: 124 LGSPCTKMELVPFVMEGERIACGSIHADNAAPAMLGNFILTRSYDPLDLIPISTP--PEL 183

Query: 232 FFVLVSPEFEAPTKKMRAALPAEVGMPHHVWNCSQAGALVAAVLQGDLMGLGKALSSDKI 291
           +  LV P  E PT   R+ L  E+ +   V      GALVA +L+ D   +G+AL  D +
Sbjct: 184 YCSLVHPHIEVPTSHARSILKKEIALSSAVRQWGNVGALVAGLLRCDYELIGRAL-EDVV 243

Query: 292 VEPRRAPLIPGMDGVKKAAIAAGAFGCTISGAGPTAVAVVDNEEKGKEIGERMAMAFLKE 351
            EP RAPLIPG   VK AA+ AGA G +I+G+GP+  A  D+  K + I + M   F K 
Sbjct: 244 AEPVRAPLIPGFYDVKHAALDAGALGGSIAGSGPSIFAFSDSLAKAETIAKAMQETFRKV 303

Query: 352 GNLKAVASVQRLDRVGARLI 365
            NL++      + R G+R++
Sbjct: 304 SNLESDIWFCPVSREGSRIL 319

BLAST of Lcy08g003900 vs. ExPASy Swiss-Prot
Match: Q8KAW7 (Homoserine kinase OS=Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) OX=194439 GN=thrB PE=3 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 8.8e-47
Identity = 134/325 (41.23%), Postives = 184/325 (56.62%), Query Frame = 0

Query: 51  SVKAFAPATVANLGPGFDFLGCAVDGLGDSVSLSV-DANVHPGEVAITNITGDNSNKLSK 110
           +V  FA ATV N+  GFD LG A+   GD V L++ D       V+IT+I GD    L  
Sbjct: 3   TVTGFASATVGNVACGFDVLGFAITEPGDEVVLALHDERRSDCPVSITSIVGD-GGALPL 62

Query: 111 NPLYNCAGIAAIEVMKML----GIRSVG-LSLSLEKGLPLGSGLGSSAASAAAAAVAVNG 170
           +P  N +    ++ ++ +    GI   G + L L+K LPL SG+GSSAASAAAA +A N 
Sbjct: 63  DPKKNTSSFVVLKFLEYIRTTKGISFDGHIDLVLKKNLPLSSGMGSSAASAAAALIAANE 122

Query: 171 LFGGKLGVDELVLAGLKSEEKVSG-YHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKE 230
           LFG      ELV   ++ E    G  HADN APA++G FILIR+Y PL+LI +K P  K 
Sbjct: 123 LFGSPCTKMELVHFAIEGERVACGSAHADNAAPAMLGNFILIRSYNPLDLITIKPP--KN 182

Query: 231 LFFVLVSPEFEAPTKKMRAALPAEVGMPHHVWNCSQAGALVAAVLQGDLMGLGKALSSDK 290
           LF  LV P  E  T   R+ LP  + +          GAL+A +L  D   +G+AL  D 
Sbjct: 183 LFGTLVHPHTELKTSFARSVLPKSIPLSTATQQWGNVGALIAGLLMEDYDLIGRAL-VDV 242

Query: 291 IVEPRRAPLIPGMDGVKKAAIAAGAFGCTISGAGPTAVAVVDNEEKGKEIGERMAMAFL- 350
           + EP+RAPLIPG + VK+AA+ AGA GC+I+G+GP+  A   + +  + +G  M  AFL 
Sbjct: 243 VAEPKRAPLIPGFNEVKQAALDAGALGCSIAGSGPSVFAFSSSRQTAEAVGSAMQSAFLH 302

Query: 351 KEGNLKAVASVQRLDRVGARLIGST 368
               L++   V  +   GAR+I +T
Sbjct: 303 SRAALQSDMWVSPICSQGARIISTT 323

BLAST of Lcy08g003900 vs. ExPASy Swiss-Prot
Match: A1BJF6 (Homoserine kinase OS=Chlorobium phaeobacteroides (strain DSM 266) OX=290317 GN=thrB PE=3 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.3e-45
Identity = 131/322 (40.68%), Postives = 183/322 (56.83%), Query Frame = 0

Query: 51  SVKAFAPATVANLGPGFDFLGCAVDGLGDSVSLSV-DANVHPGEVAITNITGDNSNKLSK 110
           +V+ FA ATV N+  GFD LG A+   GD V L++ +       V+IT I+GD    L  
Sbjct: 3   TVRGFASATVGNVACGFDVLGFAITEPGDEVILTLHEERKSECPVSITAISGD-GGALPL 62

Query: 111 NPLYNCAGIAAIEVMKML----GIRSVG-LSLSLEKGLPLGSGLGSSAASAAAAAVAVNG 170
           +P  N +    ++ ++ +    GI   G + L L+K LPL SG+GSSAASAAAA  A N 
Sbjct: 63  DPKKNTSSFVVLKFLEYIRTSKGIDFKGHIDLELKKNLPLSSGMGSSAASAAAALAAANE 122

Query: 171 LFGGKLGVDELVLAGLKSEEKVSG-YHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKE 230
           L G      ELV   ++ E    G  HADN APAI+G F+LIR+Y PL+LI +  P  + 
Sbjct: 123 LLGQPCSKMELVHFAVEGERVACGSAHADNAAPAILGNFVLIRSYAPLDLITI--PSPEH 182

Query: 231 LFFVLVSPEFEAPTKKMRAALPAEVGMPHHVWNCSQAGALVAAVLQGDLMGLGKALSSDK 290
           LF  LV P  E  T   R+ LP  + +          GAL+A +L+ D   +G+AL  D 
Sbjct: 183 LFCTLVHPHTELKTSFARSVLPRSIPLKTATQQWGNVGALIAGLLKSDYDLIGRAL-VDV 242

Query: 291 IVEPRRAPLIPGMDGVKKAAIAAGAFGCTISGAGPTAVAVVDNEEKGKEIGERMAMAF-L 350
           + EP+RAPLIPG   VK AAI  GA GC+I+G+GP+  A   ++E  + +G+ M  AF L
Sbjct: 243 VAEPKRAPLIPGFLDVKHAAIDCGALGCSIAGSGPSVFAFSSSKETAERVGQAMREAFLL 302

Query: 351 KEGNLKAVASVQRLDRVGARLI 365
            E NLK+   V  + + GA+++
Sbjct: 303 PETNLKSDMWVSPICKEGAKVL 320

BLAST of Lcy08g003900 vs. ExPASy TrEMBL
Match: A0A0A0K4Y4 (Homoserine kinase OS=Cucumis sativus OX=3659 GN=Csa_7G025730 PE=3 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 7.7e-187
Identity = 342/368 (92.93%), Postives = 360/368 (97.83%), Query Frame = 0

Query: 1   MAMISYQPPFRSLAVPPVSISNPKSVIFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATV 60
           MAM+SYQPP +SL +PPVS+SNPK V+FRC+LSLPSRTAVT+VEP+PVFSSVKAFAPATV
Sbjct: 1   MAMLSYQPPLKSLTIPPVSLSNPKPVLFRCSLSLPSRTAVTSVEPQPVFSSVKAFAPATV 60

Query: 61  ANLGPGFDFLGCAVDGLGDSVSLSVDANVHPGEVAITNITGDNSNKLSKNPLYNCAGIAA 120
           ANLGPGFDFLGCAVDGLGD VSLSVD+NVHPGEVAI++ITG+N+NKLSKNPLYNCAGIAA
Sbjct: 61  ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAISDITGNNTNKLSKNPLYNCAGIAA 120

Query: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAVAVNGLFGGKLGVDELVLAGL 180
           IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAA+AVNGLFGGKLGV+ELVLAGL
Sbjct: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL 180

Query: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240
           KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM
Sbjct: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240

Query: 241 RAALPAEVGMPHHVWNCSQAGALVAAVLQGDLMGLGKALSSDKIVEPRRAPLIPGMDGVK 300
           RAALPAEVGMPHHVWN SQAGALVAAVLQGD MGLGKALSSDKIVEPRR+PLIPGMDGVK
Sbjct: 241 RAALPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVK 300

Query: 301 KAAIAAGAFGCTISGAGPTAVAVVDNEEKGKEIGERMAMAFLKEGNLKAVASVQRLDRVG 360
           KAAIAAGAFGCTISGAGPTAVAV+DNEEKGKEIGERM MAFLKEGNLKA ASV+RLDRVG
Sbjct: 301 KAAIAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVG 360

Query: 361 ARLIGSTP 369
           ARLIGSTP
Sbjct: 361 ARLIGSTP 368

BLAST of Lcy08g003900 vs. ExPASy TrEMBL
Match: A0A5A7V1E5 (Homoserine kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00200 PE=3 SV=1)

HSP 1 Score: 660.6 bits (1703), Expect = 3.8e-186
Identity = 341/368 (92.66%), Postives = 359/368 (97.55%), Query Frame = 0

Query: 1   MAMISYQPPFRSLAVPPVSISNPKSVIFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATV 60
           MAMISYQPP +SL +PPVS+SNPK V+FRC+LSLPSR AVT+VEP+PVFSSVKAFAPATV
Sbjct: 1   MAMISYQPPLKSLTIPPVSLSNPKPVLFRCSLSLPSRAAVTSVEPQPVFSSVKAFAPATV 60

Query: 61  ANLGPGFDFLGCAVDGLGDSVSLSVDANVHPGEVAITNITGDNSNKLSKNPLYNCAGIAA 120
           ANLGPGFDFLGCAVDGLGD VSLSVD+NVHPGEVAI++ITG+N+NKLSKNPLYNCAGIAA
Sbjct: 61  ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAISDITGNNTNKLSKNPLYNCAGIAA 120

Query: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAVAVNGLFGGKLGVDELVLAGL 180
           IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAA+AVNGLFGGKLGV+ELVLAGL
Sbjct: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL 180

Query: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240
           KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPV+KELFFVLVSPEFEAPTKKM
Sbjct: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVDKELFFVLVSPEFEAPTKKM 240

Query: 241 RAALPAEVGMPHHVWNCSQAGALVAAVLQGDLMGLGKALSSDKIVEPRRAPLIPGMDGVK 300
           RAALPAEVGMPHHVWN SQAGALVAAVLQGD MGLGKALSSDKIVEPRR+PLIPGMDGVK
Sbjct: 241 RAALPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVK 300

Query: 301 KAAIAAGAFGCTISGAGPTAVAVVDNEEKGKEIGERMAMAFLKEGNLKAVASVQRLDRVG 360
           KAAIAAGAFGCTISGAGPTAVAV+DNEEKGKEIGERM MAFLKEGNLKA ASV+RLDRVG
Sbjct: 301 KAAIAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKAAASVKRLDRVG 360

Query: 361 ARLIGSTP 369
           ARLIGSTP
Sbjct: 361 ARLIGSTP 368

BLAST of Lcy08g003900 vs. ExPASy TrEMBL
Match: A0A6J1C3W0 (Homoserine kinase OS=Momordica charantia OX=3673 GN=LOC111007689 PE=3 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 5.0e-186
Identity = 340/366 (92.90%), Postives = 359/366 (98.09%), Query Frame = 0

Query: 4   ISYQPPFRSLAVPPVSISNPKSVIFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVANL 63
           I +QP  + +AVPPVS+SNPKSV+FRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVANL
Sbjct: 3   ICFQPTLKPVAVPPVSLSNPKSVLFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVANL 62

Query: 64  GPGFDFLGCAVDGLGDSVSLSVDANVHPGEVAITNITGDNSNKLSKNPLYNCAGIAAIEV 123
           GPGFDFLGCAVDGLGD VSLSVD+NVHPGEVAITNI+G+N+NKLSKNPLYNCAGIAAIEV
Sbjct: 63  GPGFDFLGCAVDGLGDFVSLSVDSNVHPGEVAITNISGNNTNKLSKNPLYNCAGIAAIEV 122

Query: 124 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAVAVNGLFGGKLGVDELVLAGLKSE 183
           MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAA+AVNGLFGGKLGV+ELVLAGLKSE
Sbjct: 123 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 182

Query: 184 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 243
           EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA
Sbjct: 183 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 242

Query: 244 LPAEVGMPHHVWNCSQAGALVAAVLQGDLMGLGKALSSDKIVEPRRAPLIPGMDGVKKAA 303
           LPAEVGMPHHVWNCSQAGALVAAVLQGDL+GLGKALSSDKIVEPRR+P+IPGMDGVKKAA
Sbjct: 243 LPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSPMIPGMDGVKKAA 302

Query: 304 IAAGAFGCTISGAGPTAVAVVDNEEKGKEIGERMAMAFLKEGNLKAVASVQRLDRVGARL 363
           IAAGAFGCTISGAGPTAVAV+D+EE+GKEIGERM MAFLKEGNLKAVA V+RLDRVGARL
Sbjct: 303 IAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVALVKRLDRVGARL 362

Query: 364 IGSTPR 370
           +GSTPR
Sbjct: 363 VGSTPR 368

BLAST of Lcy08g003900 vs. ExPASy TrEMBL
Match: A0A1S3BR18 (Homoserine kinase OS=Cucumis melo OX=3656 GN=LOC103492575 PE=3 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 1.1e-185
Identity = 340/368 (92.39%), Postives = 359/368 (97.55%), Query Frame = 0

Query: 1   MAMISYQPPFRSLAVPPVSISNPKSVIFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATV 60
           MAMISYQPP +SL +PPVS+SNPK V+FRC+LSLPSR AVT+VEP+PVFSSVKAFAPATV
Sbjct: 1   MAMISYQPPLKSLTIPPVSLSNPKPVLFRCSLSLPSRAAVTSVEPQPVFSSVKAFAPATV 60

Query: 61  ANLGPGFDFLGCAVDGLGDSVSLSVDANVHPGEVAITNITGDNSNKLSKNPLYNCAGIAA 120
           ANLGPGFDFLGCAVDGLGD VSLSVD+NVHPGEVAI++ITG+N+NKLSKNPLYNCAGIAA
Sbjct: 61  ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAISDITGNNTNKLSKNPLYNCAGIAA 120

Query: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAVAVNGLFGGKLGVDELVLAGL 180
           IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAA+AVNGLFGGKLGV+ELVLAGL
Sbjct: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL 180

Query: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240
           KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPV+KELFFVLVSPEFEAPTKKM
Sbjct: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVDKELFFVLVSPEFEAPTKKM 240

Query: 241 RAALPAEVGMPHHVWNCSQAGALVAAVLQGDLMGLGKALSSDKIVEPRRAPLIPGMDGVK 300
           RAALPAEVGMPHHVWN SQAGALVAAVLQGD MGLGKALSSDKIVEPRR+PLIPGMDGVK
Sbjct: 241 RAALPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVK 300

Query: 301 KAAIAAGAFGCTISGAGPTAVAVVDNEEKGKEIGERMAMAFLKEGNLKAVASVQRLDRVG 360
           KAAIAAGAFGCTISGAGPTAVAV+DNEE+GKEIGERM MAFLKEGNLKA ASV+RLDRVG
Sbjct: 301 KAAIAAGAFGCTISGAGPTAVAVIDNEEQGKEIGERMVMAFLKEGNLKAAASVKRLDRVG 360

Query: 361 ARLIGSTP 369
           ARLIGSTP
Sbjct: 361 ARLIGSTP 368

BLAST of Lcy08g003900 vs. ExPASy TrEMBL
Match: A0A6J1GJP3 (Homoserine kinase OS=Cucurbita moschata OX=3662 GN=LOC111454929 PE=3 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 5.0e-178
Identity = 330/368 (89.67%), Postives = 348/368 (94.57%), Query Frame = 0

Query: 1   MAMISYQPPFRSLAVPPVSISNPKSVIFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATV 60
           MAMIS+QPP +S AVP V + NPKSV   C+LSLPSRT VT VEP PVF+SVKAFAPATV
Sbjct: 1   MAMISFQPPLKSFAVPQVPLYNPKSVAVCCSLSLPSRTVVTAVEPAPVFASVKAFAPATV 60

Query: 61  ANLGPGFDFLGCAVDGLGDSVSLSVDANVHPGEVAITNITGDNSNKLSKNPLYNCAGIAA 120
           ANLGPGFDFLGCAVDGLGD VSLSVD+NV PGEVAITNITG++ NKLSKNPLYNCAGIAA
Sbjct: 61  ANLGPGFDFLGCAVDGLGDFVSLSVDSNVRPGEVAITNITGNDPNKLSKNPLYNCAGIAA 120

Query: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAVAVNGLFGGKLGVDELVLAGL 180
           IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAA+AVNGLFGGKLGV++LVLAGL
Sbjct: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEDLVLAGL 180

Query: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240
           KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM
Sbjct: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240

Query: 241 RAALPAEVGMPHHVWNCSQAGALVAAVLQGDLMGLGKALSSDKIVEPRRAPLIPGMDGVK 300
           RAALPAEVGMPHHVWNCSQAGALVAAVLQGD++GLGKALSSDKIVEP R PLIPGM GVK
Sbjct: 241 RAALPAEVGMPHHVWNCSQAGALVAAVLQGDVIGLGKALSSDKIVEPTRVPLIPGMGGVK 300

Query: 301 KAAIAAGAFGCTISGAGPTAVAVVDNEEKGKEIGERMAMAFLKEGNLKAVASVQRLDRVG 360
           KAAIAAGAFGCTISGAGPTAVAV DN+E+GKEIGE M MAF++EGNLKAVASV+RLDRVG
Sbjct: 301 KAAIAAGAFGCTISGAGPTAVAVTDNKERGKEIGESMVMAFMREGNLKAVASVKRLDRVG 360

Query: 361 ARLIGSTP 369
           ARLIGSTP
Sbjct: 361 ARLIGSTP 368

BLAST of Lcy08g003900 vs. NCBI nr
Match: XP_038888998.1 (homoserine kinase [Benincasa hispida])

HSP 1 Score: 669.8 bits (1727), Expect = 1.3e-188
Identity = 347/368 (94.29%), Postives = 362/368 (98.37%), Query Frame = 0

Query: 1   MAMISYQPPFRSLAVPPVSISNPKSVIFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATV 60
           MAMISYQPP +SLA+PPVS+SNPKSVIFRC+LSLPSRTAVT+VEPEPVFSSVKAFAPATV
Sbjct: 1   MAMISYQPPLKSLAIPPVSLSNPKSVIFRCSLSLPSRTAVTSVEPEPVFSSVKAFAPATV 60

Query: 61  ANLGPGFDFLGCAVDGLGDSVSLSVDANVHPGEVAITNITGDNSNKLSKNPLYNCAGIAA 120
           ANLGPGFDFLGCAVDGLGD VSLSVD+NVHPGEVAITNITG+N+NKLSKNPLYNCAGIAA
Sbjct: 61  ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAITNITGNNTNKLSKNPLYNCAGIAA 120

Query: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAVAVNGLFGGKLGVDELVLAGL 180
           IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAA+AVNGLFGGKLGV+ELVLAGL
Sbjct: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL 180

Query: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240
           KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM
Sbjct: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240

Query: 241 RAALPAEVGMPHHVWNCSQAGALVAAVLQGDLMGLGKALSSDKIVEPRRAPLIPGMDGVK 300
           RAALP+EVGMPHHVWN SQAGALVAAVLQGD MGLGKALSSDKIVEPRR+PLIPGMDGVK
Sbjct: 241 RAALPSEVGMPHHVWNSSQAGALVAAVLQGDAMGLGKALSSDKIVEPRRSPLIPGMDGVK 300

Query: 301 KAAIAAGAFGCTISGAGPTAVAVVDNEEKGKEIGERMAMAFLKEGNLKAVASVQRLDRVG 360
           KAAIAAGAFGCTISGAGPTAVAV+DNEEKGKEIGERM MAF+KEGNLKA ASV+RLDRVG
Sbjct: 301 KAAIAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFMKEGNLKAEASVKRLDRVG 360

Query: 361 ARLIGSTP 369
           ARLIGSTP
Sbjct: 361 ARLIGSTP 368

BLAST of Lcy08g003900 vs. NCBI nr
Match: XP_004153462.1 (homoserine kinase [Cucumis sativus] >KGN43352.1 hypothetical protein Csa_020446 [Cucumis sativus])

HSP 1 Score: 662.9 bits (1709), Expect = 1.6e-186
Identity = 342/368 (92.93%), Postives = 360/368 (97.83%), Query Frame = 0

Query: 1   MAMISYQPPFRSLAVPPVSISNPKSVIFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATV 60
           MAM+SYQPP +SL +PPVS+SNPK V+FRC+LSLPSRTAVT+VEP+PVFSSVKAFAPATV
Sbjct: 1   MAMLSYQPPLKSLTIPPVSLSNPKPVLFRCSLSLPSRTAVTSVEPQPVFSSVKAFAPATV 60

Query: 61  ANLGPGFDFLGCAVDGLGDSVSLSVDANVHPGEVAITNITGDNSNKLSKNPLYNCAGIAA 120
           ANLGPGFDFLGCAVDGLGD VSLSVD+NVHPGEVAI++ITG+N+NKLSKNPLYNCAGIAA
Sbjct: 61  ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAISDITGNNTNKLSKNPLYNCAGIAA 120

Query: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAVAVNGLFGGKLGVDELVLAGL 180
           IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAA+AVNGLFGGKLGV+ELVLAGL
Sbjct: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL 180

Query: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240
           KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM
Sbjct: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240

Query: 241 RAALPAEVGMPHHVWNCSQAGALVAAVLQGDLMGLGKALSSDKIVEPRRAPLIPGMDGVK 300
           RAALPAEVGMPHHVWN SQAGALVAAVLQGD MGLGKALSSDKIVEPRR+PLIPGMDGVK
Sbjct: 241 RAALPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVK 300

Query: 301 KAAIAAGAFGCTISGAGPTAVAVVDNEEKGKEIGERMAMAFLKEGNLKAVASVQRLDRVG 360
           KAAIAAGAFGCTISGAGPTAVAV+DNEEKGKEIGERM MAFLKEGNLKA ASV+RLDRVG
Sbjct: 301 KAAIAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKATASVKRLDRVG 360

Query: 361 ARLIGSTP 369
           ARLIGSTP
Sbjct: 361 ARLIGSTP 368

BLAST of Lcy08g003900 vs. NCBI nr
Match: KAA0059609.1 (homoserine kinase [Cucumis melo var. makuwa] >TYK08142.1 homoserine kinase [Cucumis melo var. makuwa])

HSP 1 Score: 660.6 bits (1703), Expect = 7.9e-186
Identity = 341/368 (92.66%), Postives = 359/368 (97.55%), Query Frame = 0

Query: 1   MAMISYQPPFRSLAVPPVSISNPKSVIFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATV 60
           MAMISYQPP +SL +PPVS+SNPK V+FRC+LSLPSR AVT+VEP+PVFSSVKAFAPATV
Sbjct: 1   MAMISYQPPLKSLTIPPVSLSNPKPVLFRCSLSLPSRAAVTSVEPQPVFSSVKAFAPATV 60

Query: 61  ANLGPGFDFLGCAVDGLGDSVSLSVDANVHPGEVAITNITGDNSNKLSKNPLYNCAGIAA 120
           ANLGPGFDFLGCAVDGLGD VSLSVD+NVHPGEVAI++ITG+N+NKLSKNPLYNCAGIAA
Sbjct: 61  ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAISDITGNNTNKLSKNPLYNCAGIAA 120

Query: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAVAVNGLFGGKLGVDELVLAGL 180
           IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAA+AVNGLFGGKLGV+ELVLAGL
Sbjct: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL 180

Query: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240
           KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPV+KELFFVLVSPEFEAPTKKM
Sbjct: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVDKELFFVLVSPEFEAPTKKM 240

Query: 241 RAALPAEVGMPHHVWNCSQAGALVAAVLQGDLMGLGKALSSDKIVEPRRAPLIPGMDGVK 300
           RAALPAEVGMPHHVWN SQAGALVAAVLQGD MGLGKALSSDKIVEPRR+PLIPGMDGVK
Sbjct: 241 RAALPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVK 300

Query: 301 KAAIAAGAFGCTISGAGPTAVAVVDNEEKGKEIGERMAMAFLKEGNLKAVASVQRLDRVG 360
           KAAIAAGAFGCTISGAGPTAVAV+DNEEKGKEIGERM MAFLKEGNLKA ASV+RLDRVG
Sbjct: 301 KAAIAAGAFGCTISGAGPTAVAVIDNEEKGKEIGERMVMAFLKEGNLKAAASVKRLDRVG 360

Query: 361 ARLIGSTP 369
           ARLIGSTP
Sbjct: 361 ARLIGSTP 368

BLAST of Lcy08g003900 vs. NCBI nr
Match: XP_022135832.1 (homoserine kinase [Momordica charantia])

HSP 1 Score: 660.2 bits (1702), Expect = 1.0e-185
Identity = 340/366 (92.90%), Postives = 359/366 (98.09%), Query Frame = 0

Query: 4   ISYQPPFRSLAVPPVSISNPKSVIFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVANL 63
           I +QP  + +AVPPVS+SNPKSV+FRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVANL
Sbjct: 3   ICFQPTLKPVAVPPVSLSNPKSVLFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATVANL 62

Query: 64  GPGFDFLGCAVDGLGDSVSLSVDANVHPGEVAITNITGDNSNKLSKNPLYNCAGIAAIEV 123
           GPGFDFLGCAVDGLGD VSLSVD+NVHPGEVAITNI+G+N+NKLSKNPLYNCAGIAAIEV
Sbjct: 63  GPGFDFLGCAVDGLGDFVSLSVDSNVHPGEVAITNISGNNTNKLSKNPLYNCAGIAAIEV 122

Query: 124 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAVAVNGLFGGKLGVDELVLAGLKSE 183
           MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAA+AVNGLFGGKLGV+ELVLAGLKSE
Sbjct: 123 MKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGLKSE 182

Query: 184 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 243
           EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA
Sbjct: 183 EKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKMRAA 242

Query: 244 LPAEVGMPHHVWNCSQAGALVAAVLQGDLMGLGKALSSDKIVEPRRAPLIPGMDGVKKAA 303
           LPAEVGMPHHVWNCSQAGALVAAVLQGDL+GLGKALSSDKIVEPRR+P+IPGMDGVKKAA
Sbjct: 243 LPAEVGMPHHVWNCSQAGALVAAVLQGDLVGLGKALSSDKIVEPRRSPMIPGMDGVKKAA 302

Query: 304 IAAGAFGCTISGAGPTAVAVVDNEEKGKEIGERMAMAFLKEGNLKAVASVQRLDRVGARL 363
           IAAGAFGCTISGAGPTAVAV+D+EE+GKEIGERM MAFLKEGNLKAVA V+RLDRVGARL
Sbjct: 303 IAAGAFGCTISGAGPTAVAVIDDEERGKEIGERMVMAFLKEGNLKAVALVKRLDRVGARL 362

Query: 364 IGSTPR 370
           +GSTPR
Sbjct: 363 VGSTPR 368

BLAST of Lcy08g003900 vs. NCBI nr
Match: XP_008451216.1 (PREDICTED: homoserine kinase [Cucumis melo])

HSP 1 Score: 659.1 bits (1699), Expect = 2.3e-185
Identity = 340/368 (92.39%), Postives = 359/368 (97.55%), Query Frame = 0

Query: 1   MAMISYQPPFRSLAVPPVSISNPKSVIFRCNLSLPSRTAVTTVEPEPVFSSVKAFAPATV 60
           MAMISYQPP +SL +PPVS+SNPK V+FRC+LSLPSR AVT+VEP+PVFSSVKAFAPATV
Sbjct: 1   MAMISYQPPLKSLTIPPVSLSNPKPVLFRCSLSLPSRAAVTSVEPQPVFSSVKAFAPATV 60

Query: 61  ANLGPGFDFLGCAVDGLGDSVSLSVDANVHPGEVAITNITGDNSNKLSKNPLYNCAGIAA 120
           ANLGPGFDFLGCAVDGLGD VSLSVD+NVHPGEVAI++ITG+N+NKLSKNPLYNCAGIAA
Sbjct: 61  ANLGPGFDFLGCAVDGLGDYVSLSVDSNVHPGEVAISDITGNNTNKLSKNPLYNCAGIAA 120

Query: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAVAVNGLFGGKLGVDELVLAGL 180
           IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAA+AVNGLFGGKLGV+ELVLAGL
Sbjct: 121 IEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAIAVNGLFGGKLGVEELVLAGL 180

Query: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVSPEFEAPTKKM 240
           KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPV+KELFFVLVSPEFEAPTKKM
Sbjct: 181 KSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVDKELFFVLVSPEFEAPTKKM 240

Query: 241 RAALPAEVGMPHHVWNCSQAGALVAAVLQGDLMGLGKALSSDKIVEPRRAPLIPGMDGVK 300
           RAALPAEVGMPHHVWN SQAGALVAAVLQGD MGLGKALSSDKIVEPRR+PLIPGMDGVK
Sbjct: 241 RAALPAEVGMPHHVWNSSQAGALVAAVLQGDTMGLGKALSSDKIVEPRRSPLIPGMDGVK 300

Query: 301 KAAIAAGAFGCTISGAGPTAVAVVDNEEKGKEIGERMAMAFLKEGNLKAVASVQRLDRVG 360
           KAAIAAGAFGCTISGAGPTAVAV+DNEE+GKEIGERM MAFLKEGNLKA ASV+RLDRVG
Sbjct: 301 KAAIAAGAFGCTISGAGPTAVAVIDNEEQGKEIGERMVMAFLKEGNLKAAASVKRLDRVG 360

Query: 361 ARLIGSTP 369
           ARLIGSTP
Sbjct: 361 ARLIGSTP 368

BLAST of Lcy08g003900 vs. TAIR 10
Match: AT2G17265.1 (homoserine kinase )

HSP 1 Score: 490.0 bits (1260), Expect = 1.7e-138
Identity = 269/379 (70.98%), Postives = 305/379 (80.47%), Query Frame = 0

Query: 1   MAMISYQPPFRSLAVPPVSISNPKS----------VIFRCNLSLPSRTAVTTVEPEPVFS 60
           MA + +Q P +     P+S   PKS           +FRC  S+ +  A   VEPEPVF 
Sbjct: 1   MASLCFQSPSK-----PISYFQPKSNPSPPLFAKVSVFRCRASVQTLVA---VEPEPVFV 60

Query: 61  SVKAFAPATVANLGPGFDFLGCAVDGLGDSVSLSVDANVHPGEVAITNITGDNSNKLSKN 120
           SVK FAPATVANLGPGFDFLGCAVDGLGD V+L VD +V  GEV+I+ ITG  + KLS N
Sbjct: 61  SVKTFAPATVANLGPGFDFLGCAVDGLGDHVTLRVDPSVRAGEVSISEITG-TTTKLSTN 120

Query: 121 PLYNCAGIAAIEVMKMLGIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAVAVNGLFGGKL 180
           PL NCAGIAAI  MKMLGIRSVGLSL L KGLPLGSGLGSSAASAAAAAVAVN +FG KL
Sbjct: 121 PLRNCAGIAAIATMKMLGIRSVGLSLDLHKGLPLGSGLGSSAASAAAAAVAVNEIFGRKL 180

Query: 181 GVDELVLAGLKSEEKVSGYHADNVAPAIMGGFILIRNYEPLELIRLKFPVEKELFFVLVS 240
           G D+LVLAGL+SE KVSGYHADN+APAIMGGF+LIRNYEPL+L  L+FP +K+LFFVLVS
Sbjct: 181 GSDQLVLAGLESEAKVSGYHADNIAPAIMGGFVLIRNYEPLDLKPLRFPSDKDLFFVLVS 240

Query: 241 PEFEAPTKKMRAALPAEVGMPHHVWNCSQAGALVAAVLQGDLMGLGKALSSDKIVEPRRA 300
           P+FEAPTKKMRAALP E+ M HHVWN SQA ALVAAVL+GD + LGKALSSDKIVEP RA
Sbjct: 241 PDFEAPTKKMRAALPTEIPMVHHVWNSSQAAALVAAVLEGDAVMLGKALSSDKIVEPTRA 300

Query: 301 PLIPGMDGVKKAAIAAGAFGCTISGAGPTAVAVVDNEEKGKEIGERMAMAFLKEGNLKAV 360
           PLIPGM+ VKKAA+ AGAFGCTISGAGPTAVAV+D+EEKG+ IGE+M  AF K G+LK+V
Sbjct: 301 PLIPGMEAVKKAALEAGAFGCTISGAGPTAVAVIDSEEKGQVIGEKMVEAFWKVGHLKSV 360

Query: 361 ASVQRLDRVGARLIGSTPR 370
           ASV++LD VGARL+ S  R
Sbjct: 361 ASVKKLDNVGARLVNSVSR 370

BLAST of Lcy08g003900 vs. TAIR 10
Match: AT4G35295.1 (homoserine kinase, putative / HSK, putative )

HSP 1 Score: 70.1 bits (170), Expect = 4.2e-12
Identity = 40/61 (65.57%), Postives = 46/61 (75.41%), Query Frame = 0

Query: 306 AGAFGCTISGAGPTAVAVVDNEEKGKEIGERMAMAFLKEGNLKAVASVQRLDRVGARLIG 365
           AGAFG TI     TAVAV+D  EKG EIGE+M   FLK GNLK+ ASV++LD+VGARLI 
Sbjct: 55  AGAFGRTI-----TAVAVIDTAEKGYEIGEKMVQVFLKIGNLKSTASVKKLDKVGARLIE 110

Query: 366 S 367
           S
Sbjct: 115 S 110

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L7R22.4e-13770.98Homoserine kinase OS=Arabidopsis thaliana OX=3702 GN=HSK PE=1 SV=1[more]
A4SGN01.8e-4742.24Homoserine kinase OS=Chlorobium phaeovibrioides (strain DSM 265 / 1930) OX=29031... [more]
B3QWX62.3e-4741.88Homoserine kinase OS=Chloroherpeton thalassium (strain ATCC 35110 / GB-78) OX=51... [more]
Q8KAW78.8e-4741.23Homoserine kinase OS=Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC... [more]
A1BJF61.3e-4540.68Homoserine kinase OS=Chlorobium phaeobacteroides (strain DSM 266) OX=290317 GN=t... [more]
Match NameE-valueIdentityDescription
A0A0A0K4Y47.7e-18792.93Homoserine kinase OS=Cucumis sativus OX=3659 GN=Csa_7G025730 PE=3 SV=1[more]
A0A5A7V1E53.8e-18692.66Homoserine kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00... [more]
A0A6J1C3W05.0e-18692.90Homoserine kinase OS=Momordica charantia OX=3673 GN=LOC111007689 PE=3 SV=1[more]
A0A1S3BR181.1e-18592.39Homoserine kinase OS=Cucumis melo OX=3656 GN=LOC103492575 PE=3 SV=1[more]
A0A6J1GJP35.0e-17889.67Homoserine kinase OS=Cucurbita moschata OX=3662 GN=LOC111454929 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038888998.11.3e-18894.29homoserine kinase [Benincasa hispida][more]
XP_004153462.11.6e-18692.93homoserine kinase [Cucumis sativus] >KGN43352.1 hypothetical protein Csa_020446 ... [more]
KAA0059609.17.9e-18692.66homoserine kinase [Cucumis melo var. makuwa] >TYK08142.1 homoserine kinase [Cucu... [more]
XP_022135832.11.0e-18592.90homoserine kinase [Momordica charantia][more]
XP_008451216.12.3e-18592.39PREDICTED: homoserine kinase [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT2G17265.11.7e-13870.98homoserine kinase [more]
AT4G35295.14.2e-1265.57homoserine kinase, putative / HSK, putative [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00958HOMSERKINASEcoord: 188..201
score: 52.78
coord: 59..74
score: 61.11
coord: 149..164
score: 46.18
coord: 228..245
score: 38.89
coord: 308..323
score: 47.92
NoneNo IPR availablePANTHERPTHR20861:SF8BNAA09G09000D PROTEINcoord: 14..366
NoneNo IPR availablePANTHERPTHR20861HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASEcoord: 14..366
IPR014721Ribosomal protein S5 domain 2-type fold, subgroupGENE3D3.30.230.10coord: 46..221
e-value: 3.4E-46
score: 158.9
IPR036554GHMP kinase, C-terminal domain superfamilyGENE3D3.30.70.890coord: 222..352
e-value: 1.1E-37
score: 130.8
IPR036554GHMP kinase, C-terminal domain superfamilySUPERFAMILY55060GHMP Kinase, C-terminal domaincoord: 223..338
IPR000870Homoserine kinaseTIGRFAMTIGR00191TIGR00191coord: 54..362
e-value: 2.5E-63
score: 211.7
IPR000870Homoserine kinasePIRSFPIRSF000676Homoser_kincoord: 48..363
e-value: 6.2E-93
score: 309.0
IPR000870Homoserine kinaseHAMAPMF_00384Homoser_kinasecoord: 52..364
score: 27.244003
IPR006204GHMP kinase N-terminal domainPFAMPF00288GHMP_kinases_Ncoord: 136..201
e-value: 2.1E-10
score: 40.7
IPR013750GHMP kinase, C-terminal domainPFAMPF08544GHMP_kinases_Ccoord: 264..338
e-value: 1.5E-10
score: 41.2
IPR006203GHMP kinase, ATP-binding, conserved sitePROSITEPS00627GHMP_KINASES_ATPcoord: 142..153
IPR020568Ribosomal protein S5 domain 2-type foldSUPERFAMILY54211Ribosomal protein S5 domain 2-likecoord: 51..211

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy08g003900.1Lcy08g003900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034220 ion transmembrane transport
biological_process GO:0016310 phosphorylation
biological_process GO:0009088 threonine biosynthetic process
biological_process GO:0006566 threonine metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004413 homoserine kinase activity
molecular_function GO:0015276 ligand-gated ion channel activity