Lcy08g003290 (gene) Sponge gourd (P93075) v1

Overview
NameLcy08g003290
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionPectinesterase inhibitor
LocationChr08: 4561715 .. 4562287 (-)
RNA-Seq ExpressionLcy08g003290
SyntenyLcy08g003290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATTCATCACCTATTCTTATCGTTTTGATCTTGCTTGTTTTTTCTACAGCAGCCAATATTATTGATCCGGAGACGAAGGTGGCGAATGATTTTGTTCGAGAAACATGCAACAAAACTGAAAGGAAAGAGCTTTGCATCTCTGTCGTGGATTCAGATCTTCATGAAGATTTGAAATCCAACTTGACAGGTTTTCTCACCATATTCACCAACCAATCTTTCTATGTTGTGGTTAATACTCACACCTACATTTACAATCAATTAAAAGATGGACAATTGACGAATCGAATGAAACAAGTCCTTATAGGATGTTTGGAGCAATATCGAATGAGTGAAGAAACTTTGGGGTATTTATTTGAGGAAAAACTATTGAAGCAACACATTCTAGATTTGACCCCATATTTGTCATTGGTTGCTGGTTGGTTAAACGTTTGTGAAGATGATTTTGAGGGATTTACACAAGAACCATCGACTTTGAAGTCTCTCTATGATCATGCTTTGTCACTCCTAATGCTTTCATTGGATATGTCAAATCTCATCAAATGTGGTCGTGTTGACCAACCTCTTTGCTAG

mRNA sequence

ATGAATTCATCACCTATTCTTATCGTTTTGATCTTGCTTGTTTTTTCTACAGCAGCCAATATTATTGATCCGGAGACGAAGGTGGCGAATGATTTTGTTCGAGAAACATGCAACAAAACTGAAAGGAAAGAGCTTTGCATCTCTGTCGTGGATTCAGATCTTCATGAAGATTTGAAATCCAACTTGACAGGTTTTCTCACCATATTCACCAACCAATCTTTCTATGTTGTGGTTAATACTCACACCTACATTTACAATCAATTAAAAGATGGACAATTGACGAATCGAATGAAACAAGTCCTTATAGGATGTTTGGAGCAATATCGAATGAGTGAAGAAACTTTGGGGTATTTATTTGAGGAAAAACTATTGAAGCAACACATTCTAGATTTGACCCCATATTTGTCATTGGTTGCTGGTTGGTTAAACGTTTGTGAAGATGATTTTGAGGGATTTACACAAGAACCATCGACTTTGAAGTCTCTCTATGATCATGCTTTGTCACTCCTAATGCTTTCATTGGATATGTCAAATCTCATCAAATGTGGTCGTGTTGACCAACCTCTTTGCTAG

Coding sequence (CDS)

ATGAATTCATCACCTATTCTTATCGTTTTGATCTTGCTTGTTTTTTCTACAGCAGCCAATATTATTGATCCGGAGACGAAGGTGGCGAATGATTTTGTTCGAGAAACATGCAACAAAACTGAAAGGAAAGAGCTTTGCATCTCTGTCGTGGATTCAGATCTTCATGAAGATTTGAAATCCAACTTGACAGGTTTTCTCACCATATTCACCAACCAATCTTTCTATGTTGTGGTTAATACTCACACCTACATTTACAATCAATTAAAAGATGGACAATTGACGAATCGAATGAAACAAGTCCTTATAGGATGTTTGGAGCAATATCGAATGAGTGAAGAAACTTTGGGGTATTTATTTGAGGAAAAACTATTGAAGCAACACATTCTAGATTTGACCCCATATTTGTCATTGGTTGCTGGTTGGTTAAACGTTTGTGAAGATGATTTTGAGGGATTTACACAAGAACCATCGACTTTGAAGTCTCTCTATGATCATGCTTTGTCACTCCTAATGCTTTCATTGGATATGTCAAATCTCATCAAATGTGGTCGTGTTGACCAACCTCTTTGCTAG

Protein sequence

MNSSPILIVLILLVFSTAANIIDPETKVANDFVRETCNKTERKELCISVVDSDLHEDLKSNLTGFLTIFTNQSFYVVVNTHTYIYNQLKDGQLTNRMKQVLIGCLEQYRMSEETLGYLFEEKLLKQHILDLTPYLSLVAGWLNVCEDDFEGFTQEPSTLKSLYDHALSLLMLSLDMSNLIKCGRVDQPLC
Homology
BLAST of Lcy08g003290 vs. ExPASy TrEMBL
Match: A0A6J1C7W5 (uncharacterized protein LOC111008804 OS=Momordica charantia OX=3673 GN=LOC111008804 PE=4 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 6.0e-18
Identity = 66/160 (41.25%), Postives = 85/160 (53.12%), Query Frame = 0

Query: 28  VANDFVRETCNKTERK--ELCISVVDSDLHEDLKSNLTGFLTIFTNQSFYVVVNTHTYIY 87
           VAN FV  TCNK E +  ++CI V+DSD H+DLK NL+G L IF N S     + H Y+ 
Sbjct: 9   VANSFVNNTCNKFEEEFIKVCIDVIDSDPHQDLKLNLSGLLAIFINHSIDNATDNHLYLM 68

Query: 88  NQLKDGQLTNRMKQVLIGCLEQYRMSEETLGYLFEEKLLKQHILDLTPYLSLVAGWLNVC 147
            QLK   L   ++     C+EQY    + L     E LLK    DL   LS     +  C
Sbjct: 69  EQLKRSDLDKDIRYTFNVCVEQYERGLDILKSSL-EALLKNDKDDLGLILSDAFDKIVAC 128

Query: 148 EDDFEGFTQEPSTLKSLYDHALSLLMLSLDMSNLIKCGRV 186
           EDDFE  T EP +  + Y     LL LS+  +NLI+C +V
Sbjct: 129 EDDFES-TPEPPSWLAHYLPLRKLLALSMGTANLIQCKKV 166

BLAST of Lcy08g003290 vs. ExPASy TrEMBL
Match: A0A5D3BJB5 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004170 PE=4 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 1.4e-14
Identity = 62/180 (34.44%), Postives = 91/180 (50.56%), Query Frame = 0

Query: 6   ILIVLILLVFSTAANIIDPETKVANDFVRETCNKT-ERKELCISVVDSDLHEDLKSNLTG 65
           +L+ ++ L   T A+    +    NDFVR TCN+T E K LC+S + S+   DLKSNLTG
Sbjct: 9   VLLCILSLCMLTKAD----QNTTINDFVRHTCNRTHELKNLCLSQISSEPRNDLKSNLTG 68

Query: 66  FLTIFTNQSFYVVVNTHTYIYNQLKDGQLTNRMKQVLIGCLEQYRMSEETLGYLFEEKLL 125
            L IF NQS     N  +++  ++   +++   K +L  CL+ +++    L    E    
Sbjct: 69  LLMIFVNQSISNFKNDISFLEKEINSNKISRDTKDMLEDCLQNFQIGSVNLRETMEILQT 128

Query: 126 KQHILDLTPYLSLVAGWLNVCEDDFEGFTQEPSTLKSLYDHALSLLMLSLDMSNLIKCGR 185
           K         +  V      C DDFEG T  P   +S Y+ + +LL LS+ M NLI C R
Sbjct: 129 KTGYDAEQLPVGNVGNLAVECFDDFEG-TPVPPEWQSRYNASFALLKLSMAMINLITCNR 183

BLAST of Lcy08g003290 vs. ExPASy TrEMBL
Match: A0A1R3IZD3 (TPR_REGION domain-containing protein OS=Corchorus capsularis OX=210143 GN=CCACVL1_08658 PE=4 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 3.4e-05
Identity = 48/189 (25.40%), Postives = 86/189 (45.50%), Query Frame = 0

Query: 2    NSSPILIVLILLVFSTAANIIDPETKVAN-DFVRETCNKTERKELCISVVDSDLHEDLKS 61
            +S  ++I L  LVF     +   ++K ++ D V+E C +T    LC +V+ SD      S
Sbjct: 1230 DSEEVMITLKKLVFYLDKLVAAEDSKASDKDLVQELCKRTSNSTLCSTVIKSDPRSKSTS 1289

Query: 62   NLTGFLTIFTNQSFYVVVNTHTYIYNQLKDGQLTNRMKQVLIGCLEQYRMSEETL----G 121
            +  GFL I  +Q       T  Y+ + LK         + L  C   Y  + E L    G
Sbjct: 1290 DYRGFLGIILDQILPTTKATKAYLVDLLKKNTTDKVTHECLQICNRLYDEAIEDLNSAMG 1349

Query: 122  YLFEEKLLKQHILDLTPYLSLVAGWLNVCEDDFEGFTQEPSTLKSLYDHALSLLMLSLDM 181
             ++ +   ++  + L   L         CE+ F+     PS + S+ +H +++ +++LD+
Sbjct: 1350 LVYFKN--REGYIYLNDALGSFLHEFLDCENTFKEPPSRPSPITSVDNHLVNIGLIALDI 1409

Query: 182  SNLIKCGRV 186
             NLI+C  +
Sbjct: 1410 VNLIECNHI 1416

BLAST of Lcy08g003290 vs. ExPASy TrEMBL
Match: M1DL66 (Pectinesterase inhibitor OS=Solanum tuberosum OX=4113 PE=4 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 6.5e-04
Identity = 49/183 (26.78%), Postives = 83/183 (45.36%), Query Frame = 0

Query: 2   NSSPILIVLILLVFSTAANIIDPETKVANDFVRETCNKTERKELCISVVDSDLHEDLKSN 61
           +S+P LIV +LL+  T        T V  D + + C+KT+  ++C+SV+  D      SN
Sbjct: 6   SSAPFLIVSLLLISVTL-------TSVKADLINDICSKTQENDVCLSVLRED-PRSKDSN 65

Query: 62  LTGFLTI---FTNQSFYVVVNTHTYIYNQLKDGQLTNRMKQVLIGCLEQYRMSEETLGYL 121
           L   +TI    +  +   V N    ++N+  +  L  R       C E Y  +   LGYL
Sbjct: 66  LKDLMTIAIDLSQNNTQYVRNLVAMLFNKETNSTLKTRYSL----CWENYNDAIANLGYL 125

Query: 122 FEEKLLKQHILDLTPYLSLVAGWLNVCEDDFEGFTQEPSTLKSLYDHALSLLMLSLDMSN 181
             + L  +    L  + S      + C+D+F     EP+ LK   ++   L+ + L ++ 
Sbjct: 126 -PDLLKSKDYEGLNMHASAALSEPSKCDDNFSELPVEPAQLKVANENLRELIDIILVINK 175

BLAST of Lcy08g003290 vs. NCBI nr
Match: KAG6585821.1 (hypothetical protein SDJN03_18554, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 179.5 bits (454), Expect = 2.7e-41
Identity = 94/186 (50.54%), Postives = 129/186 (69.35%), Query Frame = 0

Query: 1   MNSSPILIVLILLVFSTAANIIDPETKVANDFVRETCNKT-ERKELCISVVDSDLHEDLK 60
           MN S  ++    L+ S AAN +DPE K  +DF R TC++T E K+LCISV++S+  EDLK
Sbjct: 1   MNQS-FVVFFTYLLLSAAANTLDPEMKAGSDFTRATCDRTSELKDLCISVINSNPCEDLK 60

Query: 61  SNLTGFLTIFTNQSFYVVVNTHTYIYNQLKDGQLTNRMKQVLIGCLEQYRMSEETLGYLF 120
           SNLTGFL IF N++ +VV++ H+Y+ NQ  +GQL NR  QV   C  QY+MS+++L  L 
Sbjct: 61  SNLTGFLAIFINETLHVVIDDHSYLLNQTLNGQLDNRTLQVFRSCASQYQMSKDSLRVLL 120

Query: 121 EEKLLKQHILDLTPYLSLVAGWLNVCEDDFEGFTQEPSTLKSLYDHALSLLMLSLDMSNL 180
           E++LLKQH  DL   L  +  +L +CE +FEGF +EPS  K+ YD+  SLL+LSL  +NL
Sbjct: 121 EDELLKQHNADLNMDLGQIDNYLALCEMEFEGFIEEPSAWKTRYDYVSSLLILSLQTTNL 180

Query: 181 IKCGRV 186
           IKC R+
Sbjct: 181 IKCNRI 185

BLAST of Lcy08g003290 vs. NCBI nr
Match: KAG6599042.1 (hypothetical protein SDJN03_08820, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 176.8 bits (447), Expect = 1.8e-40
Identity = 97/187 (51.87%), Postives = 131/187 (70.05%), Query Frame = 0

Query: 1   MNSSPILIVLI-LLVFSTAANIIDPETKVANDFVRETCNKTER-KELCISVVDSDLHEDL 60
           MN S +++VL+ +LV   AANI+D E +VANDFV +TCNKTE  K+LC SV+ SD  +DL
Sbjct: 1   MNQSIVVVVLVSVLVSFVAANILDLEMEVANDFVHDTCNKTEELKDLCNSVIGSDPRDDL 60

Query: 61  KSNLTGFLTIFTNQSFYVVVNTHTYIYNQLKDGQLTNRMKQVLIGCLEQYRMSEETLGYL 120
           K+NLT  LTIF NQ+  VVV+ H+Y+YNQ  +G+L NR  +V   C+  Y+MS+++L  L
Sbjct: 61  KTNLTSLLTIFINQTLRVVVDDHSYLYNQTLNGELDNRTLEVFKSCMNLYQMSKDSLQAL 120

Query: 121 FEEKLLKQHILDLTPYLSLVAGWLNVCEDDFEGFTQEPSTLKSLYDHALSLLMLSLDMSN 180
            + +LLK    DL   L+ V  +L++CE DFEGFT E  T KS YD+  SLL+LSL M+N
Sbjct: 121 LDVELLKNKNADLNMDLAQVDNFLDLCEVDFEGFTPESLTWKSRYDYVSSLLILSLRMTN 180

Query: 181 LIKCGRV 186
           LIKC  +
Sbjct: 181 LIKCNHI 187

BLAST of Lcy08g003290 vs. NCBI nr
Match: KAG6599036.1 (hypothetical protein SDJN03_08814, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 159.5 bits (402), Expect = 2.9e-35
Identity = 77/160 (48.12%), Postives = 111/160 (69.38%), Query Frame = 0

Query: 27  KVANDFVRETCNKTER-KELCISVVDSDLHEDLKSNLTGFLTIFTNQSFYVVVNTHTYIY 86
           K  NDF+++TCN+TE  K+LCISV+DSD  EDLK N   FL IF N++  V+++ H+Y+ 
Sbjct: 2   KAPNDFIQDTCNRTEDFKDLCISVIDSDPREDLKLNSMDFLVIFINRTLQVIIDDHSYLL 61

Query: 87  NQLKDGQLTNRMKQVLIGCLEQYRMSEETLGYLFEEKLLKQHILDLTPYLSLVAGWLNVC 146
           NQ  + +L NR + + +GC+  Y+MS+++L  L  E+LLKQ   DL   L  + G+L++C
Sbjct: 62  NQTLNEELDNRTRHIFMGCMTLYQMSKDSLRVLLNEELLKQPNADLNMKLQQIEGYLDLC 121

Query: 147 EDDFEGFTQEPSTLKSLYDHALSLLMLSLDMSNLIKCGRV 186
           ++DFEGFT  PST  S YD+ +SLL+LS  M NLIKC  +
Sbjct: 122 QNDFEGFTPTPSTWTSRYDYVISLLILSFRMMNLIKCNYI 161

BLAST of Lcy08g003290 vs. NCBI nr
Match: XP_022137307.1 (uncharacterized protein LOC111008804 [Momordica charantia])

HSP 1 Score: 100.9 bits (250), Expect = 1.2e-17
Identity = 66/160 (41.25%), Postives = 85/160 (53.12%), Query Frame = 0

Query: 28  VANDFVRETCNKTERK--ELCISVVDSDLHEDLKSNLTGFLTIFTNQSFYVVVNTHTYIY 87
           VAN FV  TCNK E +  ++CI V+DSD H+DLK NL+G L IF N S     + H Y+ 
Sbjct: 9   VANSFVNNTCNKFEEEFIKVCIDVIDSDPHQDLKLNLSGLLAIFINHSIDNATDNHLYLM 68

Query: 88  NQLKDGQLTNRMKQVLIGCLEQYRMSEETLGYLFEEKLLKQHILDLTPYLSLVAGWLNVC 147
            QLK   L   ++     C+EQY    + L     E LLK    DL   LS     +  C
Sbjct: 69  EQLKRSDLDKDIRYTFNVCVEQYERGLDILKSSL-EALLKNDKDDLGLILSDAFDKIVAC 128

Query: 148 EDDFEGFTQEPSTLKSLYDHALSLLMLSLDMSNLIKCGRV 186
           EDDFE  T EP +  + Y     LL LS+  +NLI+C +V
Sbjct: 129 EDDFES-TPEPPSWLAHYLPLRKLLALSMGTANLIQCKKV 166

BLAST of Lcy08g003290 vs. NCBI nr
Match: KAA0055284.1 (putative invertase inhibitor [Cucumis melo var. makuwa] >TYJ99207.1 putative invertase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 89.7 bits (221), Expect = 2.9e-14
Identity = 62/180 (34.44%), Postives = 91/180 (50.56%), Query Frame = 0

Query: 6   ILIVLILLVFSTAANIIDPETKVANDFVRETCNKT-ERKELCISVVDSDLHEDLKSNLTG 65
           +L+ ++ L   T A+    +    NDFVR TCN+T E K LC+S + S+   DLKSNLTG
Sbjct: 9   VLLCILSLCMLTKAD----QNTTINDFVRHTCNRTHELKNLCLSQISSEPRNDLKSNLTG 68

Query: 66  FLTIFTNQSFYVVVNTHTYIYNQLKDGQLTNRMKQVLIGCLEQYRMSEETLGYLFEEKLL 125
            L IF NQS     N  +++  ++   +++   K +L  CL+ +++    L    E    
Sbjct: 69  LLMIFVNQSISNFKNDISFLEKEINSNKISRDTKDMLEDCLQNFQIGSVNLRETMEILQT 128

Query: 126 KQHILDLTPYLSLVAGWLNVCEDDFEGFTQEPSTLKSLYDHALSLLMLSLDMSNLIKCGR 185
           K         +  V      C DDFEG T  P   +S Y+ + +LL LS+ M NLI C R
Sbjct: 129 KTGYDAEQLPVGNVGNLAVECFDDFEG-TPVPPEWQSRYNASFALLKLSMAMINLITCNR 183

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1C7W56.0e-1841.25uncharacterized protein LOC111008804 OS=Momordica charantia OX=3673 GN=LOC111008... [more]
A0A5D3BJB51.4e-1434.44Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1R3IZD33.4e-0525.40TPR_REGION domain-containing protein OS=Corchorus capsularis OX=210143 GN=CCACVL... [more]
M1DL666.5e-0426.78Pectinesterase inhibitor OS=Solanum tuberosum OX=4113 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
KAG6585821.12.7e-4150.54hypothetical protein SDJN03_18554, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG6599042.11.8e-4051.87hypothetical protein SDJN03_08820, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG6599036.12.9e-3548.13hypothetical protein SDJN03_08814, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022137307.11.2e-1741.25uncharacterized protein LOC111008804 [Momordica charantia][more]
KAA0055284.12.9e-1434.44putative invertase inhibitor [Cucumis melo var. makuwa] >TYJ99207.1 putative inv... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 28..176
e-value: 2.4E-5
score: 33.8
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 31..156
e-value: 8.4E-10
score: 39.1
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 1..169
e-value: 2.9E-17
score: 60.5
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 28..182
e-value: 1.3E-16
score: 62.9
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 30..180

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy08g003290.1Lcy08g003290.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004857 enzyme inhibitor activity