Lcy08g000440 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy08g000440
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	RING-type E3 ubiquitin transferase
Location	Chr08: 746110 .. 748083 (+)
RNA-Seq Expression	Lcy08g000440
Synteny	Lcy08g000440

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAATATCCCTATTCAAGGATGGCTCTGGGAAACAACCAAAATCAAAAGTAATAGTCTCCTTAGCAACACAATGGGCAACACTGTTTGCCTCTCGTTTACAATGGAGGAAGATCACGTTTTTTAGGCGTGAAGCAATGGACAGAATCTCATCCTCCACCTCTTCTACTAGCACTGCAATCGAGTCAGGATCTCAATGGGGATTTGATTTTGTTGTTTGGTCCAAGGACATACAACATCCTACTGTGGGACCCATAAATTTATTAACCAATTGCAACACTCTATTGTGATCCCACAAACTTATTAACTCCTATTTCAATAATTTATTATGAATCCCACATACTTACTAACTCATACTCCAACTGTTGGGACAAAACAACTCGAGATTTGATAACTCAAACTCATTGTCCCAAACATCCTCTCGTAACGTTCATATTCTTTAGTGGGCCGAACCTGGCCCAAGTACATTAAGTACAAACCTTGACTAAATCCATTTTCCACAGACGAAAAAAAGTCAACAATCAGGACAAAGAAAAATCCAATCACCATTGCAAACCATTCAAAACTTTCGGTAACCTACTCCCTTAAACCACTCTCCCCGCCGCCTGGTTTTCATTTTCAATTTCAACTCTCTCTCTCTCTCTCCTGTTCTTCAAATCCGCCATGGATTTCCTCTCCAGAAAGCTCCTCTCCGACGACGACAACCTCAAGAAATTCTGCGATGATTACTGCGACCGTTTCCCTTCCGATTCTTGCCCTTCCGGTTGCCTTGAAAACTGCCCAAAACAATGTCCATATTTGTCGATTCCAATCCCACCGCCCAATTACGACGATCTCAATCCCAATTCCCCATCAAAATCGCCTCACAAACTCTCCACTTTGCTCACCGTCACCATCGCCCTTCTCTCCTTCACCTTTCTCCTCGTCCTCTGCTACGCCGTCTACGTCCGTTTCTACTCCGGCCGCCGCAGGCGCCGCCGTCGCGGCCCTGATGCCCCGCCGGAGGCCACCCACCAGCAGGAGTTTCTCGACGAAGAACACGGCCCGGTTCTGGACCACCCCATCTGGTACATCCGCACCATCGGCCTCCCGCCGGCGGTCATCGACGCCATCGCCGTCTGCAAGTTCAAATCCGGCGAGGGATTGATCGACGGAACAGAGTGCTCTGTTTGTTTGAGCGAATTTCAAGAGGATGAGACTCTGAGGCTTTTGCCCAAGTGCAGCCACGCTTTTCATCTCCCCTGCATCGACACTTGGCTTCGATCCCACACAAACTGCCCCATGTGCCGTGCTCCGGTGGTGGCTCAATCCGCCGCCGTCGCCCCTCCGCCTCCGGTGGCGGCCTCTGAAGAAACCCAGATCGGGGATTTGCAAAGGGAAGTGGGAGATGAGGCCAATTTGGGGGAAAGTTCTCAGACAAACGAAGAACAAGAACGAGATGAAATTCATTTGCCCGATGATGATTCAGAGGAAAGAGTCGGTGATTTTCAGCCCATGAGAAGATCTGTGTCTCTAGATTCTTTATCAGCTCTTCGAATCAGCTCTGTTTTCGCTGGTGTTAATGAAAAAACGGATTGTGAAGCTGAAATCGAGCAGAGAAATGGGAATGGATCGAAGAAATCATCGCAGAGGAGAGGGAATTGCTCTGCCGCTATGAACAGGTCAGTTTCTTGCAGTGGGAAGTTTCTGATGAGAAGCTCCACTCAGAGAAGTAATCACAGTTGAACTTTTTTTATTGGGTTTCTTTTTTGGTCTCTGAAGATGGAGATGTCTGAAGAAACTCTCAGGAAGCTGAGATGTTTCAAAGCTTGTTTTTTTTTTTTCTTTCAATACAGATGTTTTGAAAAGATTAATGATATATACGAGGTTGAGAACTTGAAACATGCTTTTGCTGAATTTCTGTCACTGGATGTTTGTACAAATCGTTTGGTCTATTCATCAACAAGAAACAATATGAAGAAAAATGATTACTTGAA

mRNA sequence

AAAATATCCCTATTCAAGGATGGCTCTGGGAAACAACCAAAATCAAAAGTAATAGTCTCCTTAGCAACACAATGGGCAACACTGTTTGCCTCTCGTTTACAATGGAGGAAGATCACGTTTTTTAGGCGTGAAGCAATGGACAGAATCTCATCCTCCACCTCTTCTACTAGCACTGCAATCGAGTCAGGATCTCAATGGGGATTTGATTTTGTTGTTTGGTCCAAGGACATACAACATCCTACTGTGGGACCCATAAATTTATTAACCAATTGCAACACTCTATTGTGATCCCACAAACTTATTAACTCCTATTTCAATAATTTATTATGAATCCCACATACTTACTAACTCATACTCCAACTGTTGGGACAAAACAACTCGAGATTTGATAACTCAAACTCATTGTCCCAAACATCCTCTCGTAACGTTCATATTCTTTAGTGGGCCGAACCTGGCCCAAGTACATTAAGTACAAACCTTGACTAAATCCATTTTCCACAGACGAAAAAAAGTCAACAATCAGGACAAAGAAAAATCCAATCACCATTGCAAACCATTCAAAACTTTCGGTAACCTACTCCCTTAAACCACTCTCCCCGCCGCCTGGTTTTCATTTTCAATTTCAACTCTCTCTCTCTCTCTCCTGTTCTTCAAATCCGCCATGGATTTCCTCTCCAGAAAGCTCCTCTCCGACGACGACAACCTCAAGAAATTCTGCGATGATTACTGCGACCGTTTCCCTTCCGATTCTTGCCCTTCCGGTTGCCTTGAAAACTGCCCAAAACAATGTCCATATTTGTCGATTCCAATCCCACCGCCCAATTACGACGATCTCAATCCCAATTCCCCATCAAAATCGCCTCACAAACTCTCCACTTTGCTCACCGTCACCATCGCCCTTCTCTCCTTCACCTTTCTCCTCGTCCTCTGCTACGCCGTCTACGTCCGTTTCTACTCCGGCCGCCGCAGGCGCCGCCGTCGCGGCCCTGATGCCCCGCCGGAGGCCACCCACCAGCAGGAGTTTCTCGACGAAGAACACGGCCCGGTTCTGGACCACCCCATCTGGTACATCCGCACCATCGGCCTCCCGCCGGCGGTCATCGACGCCATCGCCGTCTGCAAGTTCAAATCCGGCGAGGGATTGATCGACGGAACAGAGTGCTCTGTTTGTTTGAGCGAATTTCAAGAGGATGAGACTCTGAGGCTTTTGCCCAAGTGCAGCCACGCTTTTCATCTCCCCTGCATCGACACTTGGCTTCGATCCCACACAAACTGCCCCATGTGCCGTGCTCCGGTGGTGGCTCAATCCGCCGCCGTCGCCCCTCCGCCTCCGGTGGCGGCCTCTGAAGAAACCCAGATCGGGGATTTGCAAAGGGAAGTGGGAGATGAGGCCAATTTGGGGGAAAGTTCTCAGACAAACGAAGAACAAGAACGAGATGAAATTCATTTGCCCGATGATGATTCAGAGGAAAGAGTCGGTGATTTTCAGCCCATGAGAAGATCTGTGTCTCTAGATTCTTTATCAGCTCTTCGAATCAGCTCTGTTTTCGCTGGTGTTAATGAAAAAACGGATTGTGAAGCTGAAATCGAGCAGAGAAATGGGAATGGATCGAAGAAATCATCGCAGAGGAGAGGGAATTGCTCTGCCGCTATGAACAGGTCAGTTTCTTGCAGTGGGAAGTTTCTGATGAGAAGCTCCACTCAGAGAAGTAATCACAGTTGAACTTTTTTTATTGGGTTTCTTTTTTGGTCTCTGAAGATGGAGATGTCTGAAGAAACTCTCAGGAAGCTGAGATGTTTCAAAGCTTGTTTTTTTTTTTTCTTTCAATACAGATGTTTTGAAAAGATTAATGATATATACGAGGTTGAGAACTTGAAACATGCTTTTGCTGAATTTCTGTCACTGGATGTTTGTACAAATCGTTTGGTCTATTCATCAACAAGAAACAATATGAAGAAAAATGATTACTTGAA

Coding sequence (CDS)

ATGGATTTCCTCTCCAGAAAGCTCCTCTCCGACGACGACAACCTCAAGAAATTCTGCGATGATTACTGCGACCGTTTCCCTTCCGATTCTTGCCCTTCCGGTTGCCTTGAAAACTGCCCAAAACAATGTCCATATTTGTCGATTCCAATCCCACCGCCCAATTACGACGATCTCAATCCCAATTCCCCATCAAAATCGCCTCACAAACTCTCCACTTTGCTCACCGTCACCATCGCCCTTCTCTCCTTCACCTTTCTCCTCGTCCTCTGCTACGCCGTCTACGTCCGTTTCTACTCCGGCCGCCGCAGGCGCCGCCGTCGCGGCCCTGATGCCCCGCCGGAGGCCACCCACCAGCAGGAGTTTCTCGACGAAGAACACGGCCCGGTTCTGGACCACCCCATCTGGTACATCCGCACCATCGGCCTCCCGCCGGCGGTCATCGACGCCATCGCCGTCTGCAAGTTCAAATCCGGCGAGGGATTGATCGACGGAACAGAGTGCTCTGTTTGTTTGAGCGAATTTCAAGAGGATGAGACTCTGAGGCTTTTGCCCAAGTGCAGCCACGCTTTTCATCTCCCCTGCATCGACACTTGGCTTCGATCCCACACAAACTGCCCCATGTGCCGTGCTCCGGTGGTGGCTCAATCCGCCGCCGTCGCCCCTCCGCCTCCGGTGGCGGCCTCTGAAGAAACCCAGATCGGGGATTTGCAAAGGGAAGTGGGAGATGAGGCCAATTTGGGGGAAAGTTCTCAGACAAACGAAGAACAAGAACGAGATGAAATTCATTTGCCCGATGATGATTCAGAGGAAAGAGTCGGTGATTTTCAGCCCATGAGAAGATCTGTGTCTCTAGATTCTTTATCAGCTCTTCGAATCAGCTCTGTTTTCGCTGGTGTTAATGAAAAAACGGATTGTGAAGCTGAAATCGAGCAGAGAAATGGGAATGGATCGAAGAAATCATCGCAGAGGAGAGGGAATTGCTCTGCCGCTATGAACAGGTCAGTTTCTTGCAGTGGGAAGTTTCTGATGAGAAGCTCCACTCAGAGAAGTAATCACAGTTGA

Protein sequence

MDFLSRKLLSDDDNLKKFCDDYCDRFPSDSCPSGCLENCPKQCPYLSIPIPPPNYDDLNPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPEATHQQEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSAAVAPPPPVAASEETQIGDLQREVGDEANLGESSQTNEEQERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSALRISSVFAGVNEKTDCEAEIEQRNGNGSKKSSQRRGNCSAAMNRSVSCSGKFLMRSSTQRSNHS

Homology

[Close]

BLAST of Lcy08g000440 vs. ExPASy Swiss-Prot
Match: Q8LFY8 (RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2)

HSP 1 Score: 138.3 bits (347), Expect = 1.7e-31
Identity = 93/262 (35.50%), Postives = 138/262 (52.67%), Query Frame = 0


Query: 31  CPSGCLENCPKQCPYLSI--------------PIPPPNYDDLN-PNSPSKSPHKLSTLLT 90
           C   C  NC  +  Y +I              P P P+   +  P+  S S     +++T
Sbjct: 28  CDPICPYNCYPEPDYYTISPQLPPWSSSPQPSPCPSPSISAVYLPSQDSSSSLDAISIIT 87

Query: 91  VTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRG----PDAPPEATHQQEFLDEEHGPVLD 150
           +T A+L+   +L+  + +  +F+S    R  +G     +   +   ++EF D E    +D
Sbjct: 88  ITGAVLA---ILLTGFFLVAKFFSDSVNRVNQGTYQSDNEDNDTVMEEEFQDREQ---VD 147

Query: 151 HPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDETLRLLPKCSHAFH 210
           HPIW IRT GL  ++I++I +C +K G+GLI+ T+C VCL+EF+EDE+LRLLPKC+HAFH
Sbjct: 148 HPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDCPVCLNEFEEDESLRLLPKCNHAFH 207

Query: 211 LPCIDTWLRSHTNCPMCRAPVVAQSAAVAP-PPPVAASEETQIGDLQREVGDEANLGESS 270
           + CIDTWL SHTNCP+CRA +   S        PV  +       L+ +  DE + GE  
Sbjct: 208 ISCIDTWLSSHTNCPLCRAGIAMISVTTPRYSGPVEVTPGGSGSHLENDGVDEEDHGEI- 267

Query: 271 QTNEEQERDEIHLPDDDSEERV 273
               E   D      DDS+ R+
Sbjct: 268 ----ENRVDSDFKESDDSDIRI 278

[Close]

BLAST of Lcy08g000440 vs. ExPASy Swiss-Prot
Match: Q9LX93 (E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.7e-31
Identity = 101/257 (39.30%), Postives = 137/257 (53.31%), Query Frame = 0


Query: 35  CLENCPKQCPYLSIPIPPPNYDDLNPNSPSKSPHKLSTLLTVTIALL-SFTFLLVLCYAV 94
           CL+    +  +   P PPP         P   P +LS LL  +I ++ S    L L + +
Sbjct: 12  CLQFKDLRFCFRQYPPPPP---------PPPPPRELSLLLPTSICVVGSIILFLFLVFFL 71

Query: 95  YVRFYSGRRRRRRRGPDAPPEATHQQEFLDE-EHGPVLD-HPIWYIRTIGLPPAVIDAIA 154
           Y+      ++RR       P  T+QQE  DE E     D H +W I T+GL  + I++I 
Sbjct: 72  YLHI---TQQRRISAASVTPGDTNQQEDEDETEERDFSDFHHVWQIPTVGLHRSAINSIT 131

Query: 155 VCKFKSGEGLIDGTECSVCLSEFQEDETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAP 214
           V  FK GEG+IDGTECSVCL+EF+EDE+LRLLPKCSHAFHL CIDTWL SH NCP+CRAP
Sbjct: 132 VVGFKKGEGIIDGTECSVCLNEFEEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCRAP 191

Query: 215 VVAQSAAVAPPPPVAASEETQIGDLQREVGDEANLGESSQTNEEQERDEIHLPDDDSEER 274
           V+     +  PP     +ET+    Q +     +L     ++  +    I LP   S+  
Sbjct: 192 VL----LITEPP----HQETETNH-QPDSESSNDLRGRQDSSRSRRNHNIFLPRAQSDLA 247

Query: 275 ----VGDFQPMRRSVSL 285
                G  + +RRS S+
Sbjct: 252 NYCGSGRVENVRRSFSI 247

[Close]

BLAST of Lcy08g000440 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 1.1e-30
Identity = 124/364 (34.07%), Postives = 181/364 (49.73%), Query Frame = 0


Query: 29  DSCPSG-CLENCPKQCPYLSIPIPPPNY---DDLNPNSPSKSPHKLSTLLTVTIALLSFT 88
           + C  G C   CP+ C YL  P PPP++   DD + +S S SP     LL   I +L+  
Sbjct: 15  NDCSQGICNIYCPQWC-YLIFPPPPPSFFLDDDSSSSSSSFSP-----LLIALIGILTSA 74

Query: 89  FLLVLCYAVYVRFYSGRRRRRRRGPDAPPEATHQQEFLDEEH-GPVLDHPIWYIRT--IG 148
            +LV  Y + +  Y  R  +           T   E L+  H G         I T   G
Sbjct: 75  LILVSYYTL-ISKYCHRHHQ-----------TSSSETLNLNHNGEGFFSSTQRISTNGDG 134

Query: 149 LPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDETLRLLPKCSHAFHLPCIDTWLRS 208
           L  ++I +I V K+KSG+G +DG++CSVCLSEF+E+E+LRLLPKC+HAFHLPCIDTWL+S
Sbjct: 135 LNESMIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKS 194

Query: 209 HTNCPMCRAPVVA---QSAAVAPPPPVAASEETQIGDLQREVGDEANL---GESSQTNE- 268
           H+NCP+CRA V      +A+V     V  + ++        V  E NL   G  SQT + 
Sbjct: 195 HSNCPLCRAFVTGVNNPTASVGQNVSVVVANQSNSAHQTGSV-SEINLNLAGYESQTGDF 254

Query: 269 ---------------EQERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSALRISSVFAGV 328
                             R E+ LP++  E +  D  P+RRSVSL+S   + I+ V   +
Sbjct: 255 DSVVVIEDLEIGSRNSDARSELQLPEERRETKDEDSLPIRRSVSLNSGVVVSIADVLREI 314

Query: 329 NEKT-------DCEAEIEQRNGNGS----KKSSQRRGNCSAAMNRSVSCSGKFLMRSSTQ 353
            ++          +   E  +G+G      +++QR G  S    RS+S +G+F+     +
Sbjct: 315 EDEEGESGGVGTSQRREEGEDGDGKTIPPTEANQRSGGVSGFFVRSLS-TGRFIFSRYDR 358

[Close]

BLAST of Lcy08g000440 vs. ExPASy Swiss-Prot
Match: Q7XLY8 (E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0590900 PE=2 SV=2)

HSP 1 Score: 120.6 bits (301), Expect = 3.7e-26
Identity = 86/258 (33.33%), Postives = 121/258 (46.90%), Query Frame = 0


Query: 27  PSDSCPSG-CLENCPKQCPYLSIPIPPPNYDDLNPNSPSKSPHKLSTLLTVTIALLSFTF 86
           P+  C  G C   CP+ C Y   P PPP +D    ++   S    S L+   I +L+  F
Sbjct: 13  PTRDCSQGLCSMYCPQWC-YFIFP-PPPPFDVAGTSADDSSGPVFSPLVIAIIGVLASAF 72

Query: 87  LLVLCYAVYVRFYSGRRRRRRR-----------GPDAPPEATHQQEFLDEEHGPVLDHPI 146
           LLV  Y    ++       R R           G  A     H        HG    H  
Sbjct: 73  LLVSYYTFISKYCGTVSSLRGRVFGSSSGGAAYGGGAGSGGRH-------GHGQSRSHES 132

Query: 147 WYIR-TIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDETLRLLPKCSHAFHLP 206
           W +    GL   +I+ I VCK++ G+G +  T+CSVCL EF + E+LRLLP+CSHAFH  
Sbjct: 133 WNVSPPSGLDETLINKITVCKYRRGDGFVHTTDCSVCLGEFSDGESLRLLPRCSHAFHQQ 192

Query: 207 CIDTWLRSHTNCPMCRAPVVAQSAAVAPPPPVAASEETQIGDLQREVGDEANLGESSQTN 266
           CIDTWL+SH+NCP+CRA +   +  +A P P   +     GD   EV    +  E+    
Sbjct: 193 CIDTWLKSHSNCPLCRANITFVTVGLASPEPEGCAPGETGGDNTHEVVVVMDGLENLCEE 252

Query: 267 EEQERDEIHLPDDDSEER 272
           +++        DDD + +
Sbjct: 253 QQEAVSRASTADDDHDAK 261

[Close]

BLAST of Lcy08g000440 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 117.5 bits (293), Expect = 3.1e-25
Identity = 97/273 (35.53%), Postives = 135/273 (49.45%), Query Frame = 0


Query: 31  CPSG-CLENCPKQCPYLSIPIPPPNYDDLNPNSPSKSPHKLSTLLTVTIALLSFTFLLVL 90
           C  G C   CP+ C Y+  P PP  Y D   +S S      S LL   I +L+  F+LV 
Sbjct: 20  CSQGVCSVYCPQWC-YVIFPPPPSFYLDDEDDSSSSD---FSPLLIALIGILASAFILVS 79

Query: 91  CYAVYVRFYSGRRRRRRRGPDA------PPEATHQQEFLDEEHGPVLDHPIWYIRTI--- 150
            Y + +  Y  RRR       A        + T Q    +  +G    +     +TI   
Sbjct: 80  YYTL-ISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNGATNPN-----QTIGGG 139

Query: 151 ---GLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDETLRLLPKCSHAFHLPCIDT 210
              GL  ++I +I V K++  +G ++ ++CSVCLSEFQE+E+LRLLPKC+HAFH+PCIDT
Sbjct: 140 GGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNHAFHVPCIDT 199

Query: 211 WLRSHTNCPMCRAPVVAQSAA----VAPPPPVAASEETQIGDLQREVGDEANLGESSQTN 270
           WL+SH+NCP+CRA +V  SA     +     V  +     GD    V    +L  S   N
Sbjct: 200 WLKSHSNCPLCRAFIVTSSAVEIVDLTNQQIVTENNSISTGD-DSVVVVNLDLENSRSRN 259

Query: 271 EEQERDEIHLPDDDSEERVGDFQPMRRSVSLDS 287
           E         P +  + R G+    RRS SL+S
Sbjct: 260 ETVNEGSTPKPPEMQDSRDGE---ERRSASLNS 278

[Close]

BLAST of Lcy08g000440 vs. ExPASy TrEMBL
Match: A0A5D3C4M8 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001880 PE=3 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.5e-131
Identity = 267/359 (74.37%), Postives = 287/359 (79.94%), Query Frame = 0


Query: 1   MDFL-SRKLLS---DDDNLKKFCDDYCDRFPSDSCPSGCLENCPKQCPYLSIPIPPPNYD 60
           MDFL SRKLLS   +DD+LKK C D C  + S SCPS CLE C   C Y+ IPIPPP+ D
Sbjct: 1   MDFLSSRKLLSSDDEDDDLKKLCKD-CFDYSSYSCPSYCLEKCKNYC-YIHIPIPPPDAD 60

Query: 61  DLNPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPEAT 120
           D+N  S +KSPHKLS L+T TIALLSFTFLLVLCYA+Y RFYSGRRRR R     PPE  
Sbjct: 61  DINQTSSTKSPHKLSNLVTSTIALLSFTFLLVLCYAIYARFYSGRRRRIRLPDPPPPETH 120

Query: 121 HQQEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQE 180
           HQ EFLDEE GPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEF+E
Sbjct: 121 HQHEFLDEEQGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFEE 180

Query: 181 DETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQ-SAAVAPPPPVAASEETQIGD 240
           DETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQ SAA A PPP     ETQIG+
Sbjct: 181 DETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSSAAAASPPPPRPPAETQIGE 240

Query: 241 LQREVGDEANLGESSQTNEEQ-----ERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSAL 300
           LQRE GD ANLGESS  NEE+     E +E H  D+D EER  D QP+RRSVSLDSLSAL
Sbjct: 241 LQREEGDGANLGESSARNEEEQEQQHEEEETHFADEDLEERSSDIQPVRRSVSLDSLSAL 300

Query: 301 RISSVFAGVNEKTDCEAEIEQRNGNGSKKSSQRRG-NCSAAMNRSVSCSGKFLMRSSTQ 349
           RISSVFA VNEK D +AE  QR G   KKSSQ RG + +++MNRSVSCSGKFLMRSSTQ
Sbjct: 301 RISSVFANVNEKLDSDAETIQRKG---KKSSQGRGYSSTSSMNRSVSCSGKFLMRSSTQ 354

[Close]

BLAST of Lcy08g000440 vs. ExPASy TrEMBL
Match: A0A1S3BZS6 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103495376 PE=3 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.5e-131
Identity = 267/359 (74.37%), Postives = 287/359 (79.94%), Query Frame = 0


Query: 1   MDFL-SRKLLS---DDDNLKKFCDDYCDRFPSDSCPSGCLENCPKQCPYLSIPIPPPNYD 60
           MDFL SRKLLS   +DD+LKK C D C  + S SCPS CLE C   C Y+ IPIPPP+ D
Sbjct: 1   MDFLSSRKLLSSDDEDDDLKKLCKD-CFDYSSYSCPSYCLEKCKNYC-YIHIPIPPPDAD 60

Query: 61  DLNPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPEAT 120
           D+N  S +KSPHKLS L+T TIALLSFTFLLVLCYA+Y RFYSGRRRR R     PPE  
Sbjct: 61  DINQTSSTKSPHKLSNLVTSTIALLSFTFLLVLCYAIYARFYSGRRRRIRLPDPPPPETH 120

Query: 121 HQQEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQE 180
           HQ EFLDEE GPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEF+E
Sbjct: 121 HQHEFLDEEQGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFEE 180

Query: 181 DETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQ-SAAVAPPPPVAASEETQIGD 240
           DETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQ SAA A PPP     ETQIG+
Sbjct: 181 DETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSSAAAASPPPPRPPAETQIGE 240

Query: 241 LQREVGDEANLGESSQTNEEQ-----ERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSAL 300
           LQRE GD ANLGESS  NEE+     E +E H  D+D EER  D QP+RRSVSLDSLSAL
Sbjct: 241 LQREEGDGANLGESSARNEEEQEQQHEEEETHFADEDLEERSSDIQPVRRSVSLDSLSAL 300

Query: 301 RISSVFAGVNEKTDCEAEIEQRNGNGSKKSSQRRG-NCSAAMNRSVSCSGKFLMRSSTQ 349
           RISSVFA VNEK D +AE  QR G   KKSSQ RG + +++MNRSVSCSGKFLMRSSTQ
Sbjct: 301 RISSVFANVNEKLDSDAETIQRKG---KKSSQGRGYSSTSSMNRSVSCSGKFLMRSSTQ 354

[Close]

BLAST of Lcy08g000440 vs. ExPASy TrEMBL
Match: A0A0A0K2P1 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G066300 PE=3 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 7.0e-129
Identity = 265/361 (73.41%), Postives = 284/361 (78.67%), Query Frame = 0


Query: 1   MDFL-SRKLLS-DDDNLKKFCDDYCDRFPSDSCPSGCLENCPKQCPYLSIPIPPPNYDDL 60
           MDF  SRKLLS DDD+ K+FC D  D F SDSC S CLE C   C  ++IPI PP+ D +
Sbjct: 1   MDFFSSRKLLSEDDDDHKEFCKDCRDYF-SDSCSSDCLEKCRDYCYNINIPIRPPDADHI 60

Query: 61  NPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPEATHQ 120
           NP S  KSPHKLS L+T TIALLSFTFLLVLCYA+Y RFY+GRRRR R     PPE  HQ
Sbjct: 61  NPTSSIKSPHKLSNLVTSTIALLSFTFLLVLCYAIYARFYAGRRRRIRLPDPPPPETHHQ 120

Query: 121 QEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDE 180
            +FLDEE GPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEF+EDE
Sbjct: 121 HDFLDEEQGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFEEDE 180

Query: 181 TLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQS-AAVAPPPPVAASEETQIGDLQ 240
           TLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQS AA A PP +    ETQIG+LQ
Sbjct: 181 TLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSAAAAASPPQLRPPAETQIGELQ 240

Query: 241 REVGDEANLGESS----QTNEEQERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSALRIS 300
           RE GD ANLGESS    +  EEQE  E H  D+D EER  D QPMRRSVSLDSLSALRIS
Sbjct: 241 REEGDGANLGESSARTEEEQEEQEEQETHFADEDLEERSSDIQPMRRSVSLDSLSALRIS 300

Query: 301 SVFAGVNEKTDCEAEIEQRNGNGSKKSSQRRGNCS-AAMNRSVSCSGKFLMRSSTQRSNH 354
           S FA VNEK D EAE  QRNG   KKSSQ RG+ S ++MNRSVSCS KFLMRSSTQ +  
Sbjct: 301 SDFANVNEKMDSEAETIQRNG---KKSSQGRGHSSTSSMNRSVSCSAKFLMRSSTQINTE 357

[Close]

BLAST of Lcy08g000440 vs. ExPASy TrEMBL
Match: A0A6J1EI25 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434557 PE=3 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 3.9e-119
Identity = 245/359 (68.25%), Postives = 269/359 (74.93%), Query Frame = 0


Query: 1   MDFLSRKLLS--DDDNLKKFCDDYCDRFPSDSCPSGCLENCPKQCPYLSIPIPPPNYDDL 60
           MDFLSRKLLS  DDD+LKK C   C     DSCPS CLE CP  C   SI IPPP YD  
Sbjct: 1   MDFLSRKLLSDDDDDDLKKLCLKTCPNNSPDSCPSDCLEKCPNLCSRGSISIPPPYYD-- 60

Query: 61  NPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPEATHQ 120
                 KSPHKLS  +T TIA LSFTFLLV+CY VYVRFYSGRRRR +R PD PP  THQ
Sbjct: 61  ------KSPHKLSNWVTATIAFLSFTFLLVICYTVYVRFYSGRRRRHQR-PDPPPPTTHQ 120

Query: 121 QEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDE 180
           +EFLDEEHGPVLDHPIWYIRTIGLPPAVI+AIA+CKFK+GEGLIDGTECSVCLSEF+EDE
Sbjct: 121 EEFLDEEHGPVLDHPIWYIRTIGLPPAVINAIAICKFKTGEGLIDGTECSVCLSEFEEDE 180

Query: 181 TLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSAAVA-----PPPPVAASEETQI 240
           TLRLLPKCSHAFHLPCIDTWL+SHTNCPMCRAPVVAQS AV      PPPP+  SEETQ+
Sbjct: 181 TLRLLPKCSHAFHLPCIDTWLQSHTNCPMCRAPVVAQSTAVTPPPPPPPPPLVTSEETQM 240

Query: 241 GDLQREVGDEANLGESSQTNEEQERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSALRIS 300
            DLQREVG+ +N GESS  NEEQ+           ++   + Q +RRSVSLDSLSALRI 
Sbjct: 241 RDLQREVGNGSNSGESSLRNEEQQ----------EQQEQDEIQAVRRSVSLDSLSALRII 300

Query: 301 SVFAGVNEKTDCEAEIEQRNGNG-SKKSSQRRGNCSAAMNRSVSCSGKFLMRSSTQRSN 352
           S            A+I  RNGN   KKSSQ R N SAAMNRSVSCSG+F+MR+STQ S+
Sbjct: 301 SDL----------ADIGMRNGNDLKKKSSQERVNSSAAMNRSVSCSGEFVMRNSTQSSS 330

[Close]

BLAST of Lcy08g000440 vs. ExPASy TrEMBL
Match: A0A6J1I3F3 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111470641 PE=3 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 4.7e-117
Identity = 248/356 (69.66%), Postives = 268/356 (75.28%), Query Frame = 0


Query: 1   MDFL-SRKLLSD--DDNLKKFCDDYCDRFPSDSCPSGCLENCPKQCPYLSIPIPPPNYDD 60
           MDFL SRKLLSD  DD+L KFC + C   PSDSC S CLE CP  C Y+SI IPPP++D+
Sbjct: 1   MDFLSSRKLLSDDSDDDLNKFCPEDCFDHPSDSCFSDCLEKCPTLCSYISISIPPPDFDN 60

Query: 61  LNPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPEATH 120
            NP+S +KSPHKLS L+T+TIALLSFTFLLVLCYAVY RFYS RRR+ RR  D+PP  TH
Sbjct: 61  FNPSSAAKSPHKLSNLVTLTIALLSFTFLLVLCYAVYARFYSARRRQIRR-LDSPPPPTH 120

Query: 121 QQEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQED 180
                   H PVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEF+ED
Sbjct: 121 HH----HHHSPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFEED 180

Query: 181 ETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSAAVAPPPPVAASEETQIGDLQ 240
           ETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCR+PVVA SA   P PP A   ETQ+ +LQ
Sbjct: 181 ETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRSPVVAHSAPPTPTPPPA---ETQMANLQ 240

Query: 241 REVGDEANLGESSQTNEEQERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSALRISSVFA 300
            E  + AN GESS  NEEQ  DEIHL  D+        Q MRRSVSLD  SALRISSV A
Sbjct: 241 EEEENGANSGESSMRNEEQ--DEIHLESDN--------QAMRRSVSLDLPSALRISSVLA 300

Query: 301 GVNEKTDCEAEIEQRNGNGSKKSSQRRGNCSAAMNRSVSCSGKFLMRSSTQRSNHS 354
            VNEK D E E EQRNGN S+KS         +MNRSVSCSG FLMRS    SNHS
Sbjct: 301 VVNEKMDWEGESEQRNGNCSEKS-------EGSMNRSVSCSGGFLMRS----SNHS 327

[Close]

BLAST of Lcy08g000440 vs. NCBI nr
Match: XP_008455131.1 (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >KAA0031432.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK06883.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 479.2 bits (1232), Expect = 3.1e-131
Identity = 267/359 (74.37%), Postives = 287/359 (79.94%), Query Frame = 0


Query: 1   MDFL-SRKLLS---DDDNLKKFCDDYCDRFPSDSCPSGCLENCPKQCPYLSIPIPPPNYD 60
           MDFL SRKLLS   +DD+LKK C D C  + S SCPS CLE C   C Y+ IPIPPP+ D
Sbjct: 1   MDFLSSRKLLSSDDEDDDLKKLCKD-CFDYSSYSCPSYCLEKCKNYC-YIHIPIPPPDAD 60

Query: 61  DLNPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPEAT 120
           D+N  S +KSPHKLS L+T TIALLSFTFLLVLCYA+Y RFYSGRRRR R     PPE  
Sbjct: 61  DINQTSSTKSPHKLSNLVTSTIALLSFTFLLVLCYAIYARFYSGRRRRIRLPDPPPPETH 120

Query: 121 HQQEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQE 180
           HQ EFLDEE GPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEF+E
Sbjct: 121 HQHEFLDEEQGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFEE 180

Query: 181 DETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQ-SAAVAPPPPVAASEETQIGD 240
           DETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQ SAA A PPP     ETQIG+
Sbjct: 181 DETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSSAAAASPPPPRPPAETQIGE 240

Query: 241 LQREVGDEANLGESSQTNEEQ-----ERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSAL 300
           LQRE GD ANLGESS  NEE+     E +E H  D+D EER  D QP+RRSVSLDSLSAL
Sbjct: 241 LQREEGDGANLGESSARNEEEQEQQHEEEETHFADEDLEERSSDIQPVRRSVSLDSLSAL 300

Query: 301 RISSVFAGVNEKTDCEAEIEQRNGNGSKKSSQRRG-NCSAAMNRSVSCSGKFLMRSSTQ 349
           RISSVFA VNEK D +AE  QR G   KKSSQ RG + +++MNRSVSCSGKFLMRSSTQ
Sbjct: 301 RISSVFANVNEKLDSDAETIQRKG---KKSSQGRGYSSTSSMNRSVSCSGKFLMRSSTQ 354

[Close]

BLAST of Lcy08g000440 vs. NCBI nr
Match: XP_004137067.1 (RING-H2 finger protein ATL54 [Cucumis sativus] >KGN43773.1 hypothetical protein Csa_017129 [Cucumis sativus])

HSP 1 Score: 470.3 bits (1209), Expect = 1.4e-128
Identity = 265/361 (73.41%), Postives = 284/361 (78.67%), Query Frame = 0


Query: 1   MDFL-SRKLLS-DDDNLKKFCDDYCDRFPSDSCPSGCLENCPKQCPYLSIPIPPPNYDDL 60
           MDF  SRKLLS DDD+ K+FC D  D F SDSC S CLE C   C  ++IPI PP+ D +
Sbjct: 1   MDFFSSRKLLSEDDDDHKEFCKDCRDYF-SDSCSSDCLEKCRDYCYNINIPIRPPDADHI 60

Query: 61  NPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPEATHQ 120
           NP S  KSPHKLS L+T TIALLSFTFLLVLCYA+Y RFY+GRRRR R     PPE  HQ
Sbjct: 61  NPTSSIKSPHKLSNLVTSTIALLSFTFLLVLCYAIYARFYAGRRRRIRLPDPPPPETHHQ 120

Query: 121 QEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDE 180
            +FLDEE GPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEF+EDE
Sbjct: 121 HDFLDEEQGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFEEDE 180

Query: 181 TLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQS-AAVAPPPPVAASEETQIGDLQ 240
           TLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQS AA A PP +    ETQIG+LQ
Sbjct: 181 TLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSAAAAASPPQLRPPAETQIGELQ 240

Query: 241 REVGDEANLGESS----QTNEEQERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSALRIS 300
           RE GD ANLGESS    +  EEQE  E H  D+D EER  D QPMRRSVSLDSLSALRIS
Sbjct: 241 REEGDGANLGESSARTEEEQEEQEEQETHFADEDLEERSSDIQPMRRSVSLDSLSALRIS 300

Query: 301 SVFAGVNEKTDCEAEIEQRNGNGSKKSSQRRGNCS-AAMNRSVSCSGKFLMRSSTQRSNH 354
           S FA VNEK D EAE  QRNG   KKSSQ RG+ S ++MNRSVSCS KFLMRSSTQ +  
Sbjct: 301 SDFANVNEKMDSEAETIQRNG---KKSSQGRGHSSTSSMNRSVSCSAKFLMRSSTQINTE 357

[Close]

BLAST of Lcy08g000440 vs. NCBI nr
Match: XP_038888113.1 (E3 ubiquitin-protein ligase RING1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 466.8 bits (1200), Expect = 1.6e-127
Identity = 258/353 (73.09%), Postives = 283/353 (80.17%), Query Frame = 0


Query: 1   MDFLS-RKLLSDD-DNLKKFCDDYCDRFPSDSCPSGCLENCPKQCPYLSIPIPPPNYDDL 60
           MDF+S RKLLSDD D+++KFC D C  + + SC S C+E CPK C Y+S+ IPPP     
Sbjct: 1   MDFISTRKLLSDDEDDIEKFCKD-CHEYLTGSCSSDCIEKCPKLC-YISMSIPPP----- 60

Query: 61  NPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPEAT-H 120
               PSKSPHKLS L+T TIALLSFTFLLVLCYA+Y RFYSGRRRR RR PD PP  T H
Sbjct: 61  ----PSKSPHKLSNLVTSTIALLSFTFLLVLCYAIYARFYSGRRRRIRR-PDPPPPVTHH 120

Query: 121 QQEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQED 180
           Q +FLDEE GPVLDHPIWYIRT+GLPPAVI+AIAVCKFKSGEGLIDGTECSVCLSEF+ED
Sbjct: 121 QHDFLDEEQGPVLDHPIWYIRTVGLPPAVIEAIAVCKFKSGEGLIDGTECSVCLSEFEED 180

Query: 181 ETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSAAVAPPPPVAASEETQIGDLQ 240
           ETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQ  A A PPP     ETQ+ + Q
Sbjct: 181 ETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQPTAAASPPPPQPPAETQMREWQ 240

Query: 241 REVGDEANLGESSQTNEEQERD--EIHLPDDDSEERVGDFQPMRRSVSLDSLSALRISSV 300
           R  GD ANLGESS  NEEQE++  EIH P++D EE     QP+RRS+SLDSLSALRISSV
Sbjct: 241 RGEGDGANLGESSTRNEEQEQEQVEIHFPEEDLEE----IQPVRRSISLDSLSALRISSV 300

Query: 301 FAGVNEKTDCEAEIEQRNGNGSKKSSQRRGNCSAAMNRSVSCSGKFLMRSSTQ 349
           FA VNEK D EAE  QRNGN SKKS Q RGN S+AMNRSVSCSGKFLMR S +
Sbjct: 301 FANVNEKMDSEAESMQRNGNESKKSYQGRGNSSSAMNRSVSCSGKFLMRRSAE 337

[Close]

BLAST of Lcy08g000440 vs. NCBI nr
Match: XP_038888112.1 (E3 ubiquitin-protein ligase RING1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 443.7 bits (1140), Expect = 1.5e-120
Identity = 245/337 (72.70%), Postives = 269/337 (79.82%), Query Frame = 0


Query: 1   MDFLS-RKLLSDD-DNLKKFCDDYCDRFPSDSCPSGCLENCPKQCPYLSIPIPPPNYDDL 60
           MDF+S RKLLSDD D+++KFC D C  + + SC S C+E CPK C Y+S+ IPPP     
Sbjct: 1   MDFISTRKLLSDDEDDIEKFCKD-CHEYLTGSCSSDCIEKCPKLC-YISMSIPPP----- 60

Query: 61  NPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPEAT-H 120
               PSKSPHKLS L+T TIALLSFTFLLVLCYA+Y RFYSGRRRR RR PD PP  T H
Sbjct: 61  ----PSKSPHKLSNLVTSTIALLSFTFLLVLCYAIYARFYSGRRRRIRR-PDPPPPVTHH 120

Query: 121 QQEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQED 180
           Q +FLDEE GPVLDHPIWYIRT+GLPPAVI+AIAVCKFKSGEGLIDGTECSVCLSEF+ED
Sbjct: 121 QHDFLDEEQGPVLDHPIWYIRTVGLPPAVIEAIAVCKFKSGEGLIDGTECSVCLSEFEED 180

Query: 181 ETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSAAVAPPPPVAASEETQIGDLQ 240
           ETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQ  A A PPP     ETQ+ + Q
Sbjct: 181 ETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQPTAAASPPPPQPPAETQMREWQ 240

Query: 241 REVGDEANLGESSQTNEEQERD--EIHLPDDDSEERVGDFQPMRRSVSLDSLSALRISSV 300
           R  GD ANLGESS  NEEQE++  EIH P++D EE     QP+RRS+SLDSLSALRISSV
Sbjct: 241 RGEGDGANLGESSTRNEEQEQEQVEIHFPEEDLEE----IQPVRRSISLDSLSALRISSV 300

Query: 301 FAGVNEKTDCEAEIEQRNGNGSKKSSQRRGNCSAAMN 333
           FA VNEK D EAE  QRNGN SKKS Q RGN S+AMN
Sbjct: 301 FANVNEKMDSEAESMQRNGNESKKSYQGRGNSSSAMN 321

[Close]

BLAST of Lcy08g000440 vs. NCBI nr
Match: KAG7022535.1 (RING-H2 finger protein ATL54, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 441.4 bits (1134), Expect = 7.2e-120
Identity = 249/360 (69.17%), Postives = 270/360 (75.00%), Query Frame = 0


Query: 1   MDFLSRKLLS--DDDNLKKFCDDYCDRFPSDSCPSGCLENCPKQCPYLSIPIPPPNYDDL 60
           MDFLSRKLLS  DDD+LKK C   C  +  DSCPS CLE CP  C   SI IPPP YD  
Sbjct: 1   MDFLSRKLLSDDDDDDLKKLCLKTCPNYSPDSCPSNCLEKCPNLCSRGSISIPPPYYD-- 60

Query: 61  NPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPEATHQ 120
                 KSPHKLS  +T TIA LSFTFLLVLCY VYVRFYSGRRRR +R PD PP  THQ
Sbjct: 61  ------KSPHKLSNWVTATIAFLSFTFLLVLCYTVYVRFYSGRRRRHQR-PDPPPPTTHQ 120

Query: 121 QEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDE 180
           +EFLDEEHGPVLDHPIWYIRTIGLPPAVI+AIA+CKFK+GEGLIDGTECSVCLSEF+EDE
Sbjct: 121 EEFLDEEHGPVLDHPIWYIRTIGLPPAVINAIAICKFKTGEGLIDGTECSVCLSEFEEDE 180

Query: 181 TLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSAAVA-----PPPPVAASEETQI 240
           TLRLLPKCSHAFHLPCIDTWL+SHTNCPMCRAPVVAQS AV      PPPP+  SEETQ+
Sbjct: 181 TLRLLPKCSHAFHLPCIDTWLQSHTNCPMCRAPVVAQSTAVTPPPPPPPPPLVTSEETQM 240

Query: 241 GDLQREVGDEANLGESSQTNEE-QERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSALRI 300
            DLQREVG+ +N GESS  NEE QE+DEI              QP+RRSVSLDSLSALRI
Sbjct: 241 RDLQREVGNGSNSGESSLRNEEQQEQDEI--------------QPVRRSVSLDSLSALRI 300

Query: 301 SSVFAGVNEKTDCEAEIEQRNGNG-SKKSSQRRGNCSAAMNRSVSCSGKFLMRSSTQRSN 352
            S            A+I  RNGN   KKS Q R N SA MNRSVSCSG+F+MR+STQ S+
Sbjct: 301 ISDL----------ADIGMRNGNDLKKKSCQERVNSSAVMNRSVSCSGEFVMRNSTQSSS 327

[Close]

BLAST of Lcy08g000440 vs. TAIR 10
Match: AT4G33565.1 (RING/U-box superfamily protein )

HSP 1 Score: 176.4 bits (446), Expect = 4.0e-44
Identity = 129/346 (37.28%), Postives = 182/346 (52.60%), Query Frame = 0


Query: 1   MDFLSRKLLSDDDNLKKFCDDYC------DRFPSDSCPSGCLENCPKQCPYLSIPIPPPN 60
           M FL R LL D    + F D  C      +      C S CL+ CP  C  + I      
Sbjct: 54  MRFLHRFLLEDMPK-ETFSDSLCKNNCIQELKTLGYCSSYCLDECPSICEIVVI------ 113

Query: 61  YDDLNPNSPSKSPHKLSTLLTVTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRGPDAPPE 120
              L+P +       L  +L ++ + +  T  L+    +  +FY  RRR          E
Sbjct: 114 -SGLSPPAEIHHDDNLKRILIISASSIITTLFLLTLLVLCFKFYYRRRRSITTSRRWSME 173

Query: 121 ATHQQEFLDEEHGPVLDHPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEF 180
                +F D     ++DHPIW+IRTIGL P VI +I VC++   +G+++GT+CSVCLSEF
Sbjct: 174 EARNWDF-DGPAPVIVDHPIWHIRTIGLNPTVISSIKVCQYSKKDGVVEGTDCSVCLSEF 233

Query: 181 QEDETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAPVVAQSAAVAPPPPVAASEETQIG 240
           +E+ETLRLLPKC HAFHL CIDTWLRSHTNCP+CRAP+V  +  +         EE  + 
Sbjct: 234 EEEETLRLLPKCKHAFHLYCIDTWLRSHTNCPLCRAPIVEANTMI--DDHSEGLEEISV- 293

Query: 241 DLQREVGDEANLGESSQTNEEQERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSALRISS 300
            +  E GD+ +     +  EE++    ++  D  E +    Q +RRSVSLDSLS LR+S 
Sbjct: 294 MIPEENGDDTD----EEIEEERDGFVSNISRDHGESQQ---QRVRRSVSLDSLSGLRVSE 353

Query: 301 VFAGVNEKTDCEAEIEQRNGNGSKKSSQRRGNCSAAMNRSVSCSGK 341
           V  G  ++     ++++ N  GS +SS         + RS+S SGK
Sbjct: 354 VVVGREKE-----KLKRGNNIGSGRSS--------LLKRSISYSGK 367

[Close]

BLAST of Lcy08g000440 vs. TAIR 10
Match: AT1G72220.1 (RING/U-box superfamily protein )

HSP 1 Score: 138.3 bits (347), Expect = 1.2e-32
Identity = 93/262 (35.50%), Postives = 138/262 (52.67%), Query Frame = 0


Query: 31  CPSGCLENCPKQCPYLSI--------------PIPPPNYDDLN-PNSPSKSPHKLSTLLT 90
           C   C  NC  +  Y +I              P P P+   +  P+  S S     +++T
Sbjct: 28  CDPICPYNCYPEPDYYTISPQLPPWSSSPQPSPCPSPSISAVYLPSQDSSSSLDAISIIT 87

Query: 91  VTIALLSFTFLLVLCYAVYVRFYSGRRRRRRRG----PDAPPEATHQQEFLDEEHGPVLD 150
           +T A+L+   +L+  + +  +F+S    R  +G     +   +   ++EF D E    +D
Sbjct: 88  ITGAVLA---ILLTGFFLVAKFFSDSVNRVNQGTYQSDNEDNDTVMEEEFQDREQ---VD 147

Query: 151 HPIWYIRTIGLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDETLRLLPKCSHAFH 210
           HPIW IRT GL  ++I++I +C +K G+GLI+ T+C VCL+EF+EDE+LRLLPKC+HAFH
Sbjct: 148 HPIWLIRTTGLQQSIINSITICNYKRGDGLIERTDCPVCLNEFEEDESLRLLPKCNHAFH 207

Query: 211 LPCIDTWLRSHTNCPMCRAPVVAQSAAVAP-PPPVAASEETQIGDLQREVGDEANLGESS 270
           + CIDTWL SHTNCP+CRA +   S        PV  +       L+ +  DE + GE  
Sbjct: 208 ISCIDTWLSSHTNCPLCRAGIAMISVTTPRYSGPVEVTPGGSGSHLENDGVDEEDHGEI- 267

Query: 271 QTNEEQERDEIHLPDDDSEERV 273
               E   D      DDS+ R+
Sbjct: 268 ----ENRVDSDFKESDDSDIRI 278

[Close]

BLAST of Lcy08g000440 vs. TAIR 10
Match: AT5G10380.1 (RING/U-box superfamily protein )

HSP 1 Score: 138.3 bits (347), Expect = 1.2e-32
Identity = 101/257 (39.30%), Postives = 137/257 (53.31%), Query Frame = 0


Query: 35  CLENCPKQCPYLSIPIPPPNYDDLNPNSPSKSPHKLSTLLTVTIALL-SFTFLLVLCYAV 94
           CL+    +  +   P PPP         P   P +LS LL  +I ++ S    L L + +
Sbjct: 12  CLQFKDLRFCFRQYPPPPP---------PPPPPRELSLLLPTSICVVGSIILFLFLVFFL 71

Query: 95  YVRFYSGRRRRRRRGPDAPPEATHQQEFLDE-EHGPVLD-HPIWYIRTIGLPPAVIDAIA 154
           Y+      ++RR       P  T+QQE  DE E     D H +W I T+GL  + I++I 
Sbjct: 72  YLHI---TQQRRISAASVTPGDTNQQEDEDETEERDFSDFHHVWQIPTVGLHRSAINSIT 131

Query: 155 VCKFKSGEGLIDGTECSVCLSEFQEDETLRLLPKCSHAFHLPCIDTWLRSHTNCPMCRAP 214
           V  FK GEG+IDGTECSVCL+EF+EDE+LRLLPKCSHAFHL CIDTWL SH NCP+CRAP
Sbjct: 132 VVGFKKGEGIIDGTECSVCLNEFEEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCRAP 191

Query: 215 VVAQSAAVAPPPPVAASEETQIGDLQREVGDEANLGESSQTNEEQERDEIHLPDDDSEER 274
           V+     +  PP     +ET+    Q +     +L     ++  +    I LP   S+  
Sbjct: 192 VL----LITEPP----HQETETNH-QPDSESSNDLRGRQDSSRSRRNHNIFLPRAQSDLA 247

Query: 275 ----VGDFQPMRRSVSL 285
                G  + +RRS S+
Sbjct: 252 NYCGSGRVENVRRSFSI 247

[Close]

BLAST of Lcy08g000440 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 135.6 bits (340), Expect = 7.9e-32
Identity = 124/364 (34.07%), Postives = 181/364 (49.73%), Query Frame = 0


Query: 29  DSCPSG-CLENCPKQCPYLSIPIPPPNY---DDLNPNSPSKSPHKLSTLLTVTIALLSFT 88
           + C  G C   CP+ C YL  P PPP++   DD + +S S SP     LL   I +L+  
Sbjct: 15  NDCSQGICNIYCPQWC-YLIFPPPPPSFFLDDDSSSSSSSFSP-----LLIALIGILTSA 74

Query: 89  FLLVLCYAVYVRFYSGRRRRRRRGPDAPPEATHQQEFLDEEH-GPVLDHPIWYIRT--IG 148
            +LV  Y + +  Y  R  +           T   E L+  H G         I T   G
Sbjct: 75  LILVSYYTL-ISKYCHRHHQ-----------TSSSETLNLNHNGEGFFSSTQRISTNGDG 134

Query: 149 LPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDETLRLLPKCSHAFHLPCIDTWLRS 208
           L  ++I +I V K+KSG+G +DG++CSVCLSEF+E+E+LRLLPKC+HAFHLPCIDTWL+S
Sbjct: 135 LNESMIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAFHLPCIDTWLKS 194

Query: 209 HTNCPMCRAPVVA---QSAAVAPPPPVAASEETQIGDLQREVGDEANL---GESSQTNE- 268
           H+NCP+CRA V      +A+V     V  + ++        V  E NL   G  SQT + 
Sbjct: 195 HSNCPLCRAFVTGVNNPTASVGQNVSVVVANQSNSAHQTGSV-SEINLNLAGYESQTGDF 254

Query: 269 ---------------EQERDEIHLPDDDSEERVGDFQPMRRSVSLDSLSALRISSVFAGV 328
                             R E+ LP++  E +  D  P+RRSVSL+S   + I+ V   +
Sbjct: 255 DSVVVIEDLEIGSRNSDARSELQLPEERRETKDEDSLPIRRSVSLNSGVVVSIADVLREI 314

Query: 329 NEKT-------DCEAEIEQRNGNGS----KKSSQRRGNCSAAMNRSVSCSGKFLMRSSTQ 353
            ++          +   E  +G+G      +++QR G  S    RS+S +G+F+     +
Sbjct: 315 EDEEGESGGVGTSQRREEGEDGDGKTIPPTEANQRSGGVSGFFVRSLS-TGRFIFSRYDR 358

[Close]

BLAST of Lcy08g000440 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 117.5 bits (293), Expect = 2.2e-26
Identity = 97/273 (35.53%), Postives = 135/273 (49.45%), Query Frame = 0


Query: 31  CPSG-CLENCPKQCPYLSIPIPPPNYDDLNPNSPSKSPHKLSTLLTVTIALLSFTFLLVL 90
           C  G C   CP+ C Y+  P PP  Y D   +S S      S LL   I +L+  F+LV 
Sbjct: 20  CSQGVCSVYCPQWC-YVIFPPPPSFYLDDEDDSSSSD---FSPLLIALIGILASAFILVS 79

Query: 91  CYAVYVRFYSGRRRRRRRGPDA------PPEATHQQEFLDEEHGPVLDHPIWYIRTI--- 150
            Y + +  Y  RRR       A        + T Q    +  +G    +     +TI   
Sbjct: 80  YYTL-ISKYCHRRRHNSSSTSAAAINRISSDYTWQGTNNNNNNGATNPN-----QTIGGG 139

Query: 151 ---GLPPAVIDAIAVCKFKSGEGLIDGTECSVCLSEFQEDETLRLLPKCSHAFHLPCIDT 210
              GL  ++I +I V K++  +G ++ ++CSVCLSEFQE+E+LRLLPKC+HAFH+PCIDT
Sbjct: 140 GGDGLDESLIKSITVYKYRKMDGFVESSDCSVCLSEFQENESLRLLPKCNHAFHVPCIDT 199

Query: 211 WLRSHTNCPMCRAPVVAQSAA----VAPPPPVAASEETQIGDLQREVGDEANLGESSQTN 270
           WL+SH+NCP+CRA +V  SA     +     V  +     GD    V    +L  S   N
Sbjct: 200 WLKSHSNCPLCRAFIVTSSAVEIVDLTNQQIVTENNSISTGD-DSVVVVNLDLENSRSRN 259

Query: 271 EEQERDEIHLPDDDSEERVGDFQPMRRSVSLDS 287
           E         P +  + R G+    RRS SL+S
Sbjct: 260 ETVNEGSTPKPPEMQDSRDGE---ERRSASLNS 278

The following BLAST results are available for this feature:

BLAST of Lcy08g000440 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LFY8	1.7e-31	35.50	RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2	[more]
Q9LX93	1.7e-31	39.30	E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 ...	[more]
Q9LF64	1.1e-30	34.07	RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1	[more]
Q7XLY8	3.7e-26	33.33	E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=3994...	[more]
Q9SRQ8	3.1e-25	35.53	RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2	[more]

BLAST of Lcy08g000440 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3C4M8	1.5e-131	74.37	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3BZS6	1.5e-131	74.37	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103495376 PE=3 ...	[more]
A0A0A0K2P1	7.0e-129	73.41	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_7G066300 PE...	[more]
A0A6J1EI25	3.9e-119	68.25	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434557...	[more]
A0A6J1I3F3	4.7e-117	69.66	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111470641 P...	[more]

BLAST of Lcy08g000440 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008455131.1	3.1e-131	74.37	PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >KAA0031432.1 E...	[more]
XP_004137067.1	1.4e-128	73.41	RING-H2 finger protein ATL54 [Cucumis sativus] >KGN43773.1 hypothetical protein ...	[more]
XP_038888113.1	1.6e-127	73.09	E3 ubiquitin-protein ligase RING1-like isoform X2 [Benincasa hispida]	[more]
XP_038888112.1	1.5e-120	72.70	E3 ubiquitin-protein ligase RING1-like isoform X1 [Benincasa hispida]	[more]
KAG7022535.1	7.2e-120	69.17	RING-H2 finger protein ATL54, partial [Cucurbita argyrosperma subsp. argyrosperm...	[more]

BLAST of Lcy08g000440 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G33565.1	4.0e-44	37.28	RING/U-box superfamily protein	[more]
AT1G72220.1	1.2e-32	35.50	RING/U-box superfamily protein	[more]
AT5G10380.1	1.2e-32	39.30	RING/U-box superfamily protein	[more]
AT5G17600.1	7.9e-32	34.07	RING/U-box superfamily protein	[more]
AT3G03550.1	2.2e-26	35.53	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 167..208 e-value: 1.8E-7 score: 40.8
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 166..209 e-value: 4.3E-13 score: 49.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 167..209 score: 12.872856
None	No IPR available	SMART	SM01197	FANCL_C_2	coord: 163..216 e-value: 0.0084 score: -1.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 314..331
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 308..331
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 252..276
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 243..277
None	No IPR available	PANTHER	PTHR45676:SF126	RING-H2 FINGER PROTEIN ATL54	coord: 28..343
None	No IPR available	CDD	cd16454	RING-H2_PA-TM-RING	coord: 166..209 e-value: 1.85513E-15 score: 67.7121
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 161..215
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 144..218 e-value: 1.3E-21 score: 77.6
IPR044675	E3 ubiquitin-protein ligase RING1-like	PANTHER	PTHR45676	RING-H2 FINGER PROTEIN ATL51-RELATED	coord: 28..343

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy08g000440.1	Lcy08g000440.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane