Lcy07g014030 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy07g014030
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Eukaryotic aspartyl protease family protein
Location	Chr07: 43181900 .. 43184073 (-)
RNA-Seq Expression	Lcy07g014030
Synteny	Lcy07g014030

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCAGAGAGTGTAACAGATGGTGGAAGTTTGTTATAAAAACAGGGGAAGAAGAAACGCCATTATTAGGGCATTCTAAATTTACCACTTTTCTGTTGAGTTTCTGAATCTTCCTTCCCCTTTTTAAGTCCGCCATTGCCATCTTTCTCCTTGCTCCATCTCTCTAACATTGCAATATCTGTTTGTGTTTGTATGTTAGATTACAGGAAGCCATTAATTATGTCGCCTGTTTCAAATTTTTCGATCCTTTTCTTCTCCATCTTCTTCTTCCTCTCCGTTCACATTGCATTCGGCGACGGCAATGATGACCAAGAAGAGGCGGTCAGGCTGGAACTGCTGCACCGCCACCATCCACAAGTCTCCGAGAAGCTTCACGGCGAGGCGAAGCTTCAGGGAGCCAACGAACGCGTGAAGGATATTCACGATCACGACCACAATCGCCACCGTTTCATCTCGGAGTCGCTGAATCGGACTCAAACCGAGAAGAAATTGATGGCGGAGGGGCAGATGACGACGGAGCCTTCGTCGTCGGCAACGCCGATCGGGATGAAAATGATCTCAGGCGCCGATTATGGAGCCAGCGAGTACTTCGTGGAATTGAAAGTCGGAACGCCGCCGCAGAAGTGCATGTTGATCGCGGATACCGGAAGTGACCTAACCTGGGTAAAATGCAGATACCGGCGGTGCATGGGGAATTGCAGCGCCAATCCCGCTCATATGATTCGAAACGAAAAGAAAGAGAGATTCCCTAGGGCGTTTCATGCCAATCGATCTTCCTCTTTCCAGCCGATCAGTTGCGGCAAGAAATGTATGGATGATTATGCCACTCTCTTCGCCCTCCCGGATTGTAAGACCCCTACCAGCCCCTGTCGCTATGATTACGGGTAGGTACGTACATTGATTCAATGATTTTCCCCAATATTGATCTCAAACCAAAACAAAAACTCATTTCAATGTTTAATTAATTATAACTTAGAGTCTGCATACCAGCATGTAGCCAATTAGTAGTTTCGAAAATTTCTGATTCAAATTTCTGATTCTAGAGTATGTATGTTTTGTTTGAATTAGTTTGTGAAAAAGAAAGAAGAAGAACAAATGAGTTAATAATTTTGATTTGGTACGAAAACAAACAGCTACTCAGGTGGATCAAGTGCAAGGGGAATATTCGCAATCGAGACCCTAACGGTGGCCCTAACAAACGGAAAAGAAAAGCAACTCCACGACACTTTAATCGGCTGCACCGAATCAGCCACAGGCAGAGTCTTCAACGGAGCCGACGGCGTCCTTGGCTTAGGGACTACCGTATTCTCCTTCCCCTACAGAGCCAGACAAAACGCCAACGGCGGCGGCTTCTCCTACTGCCTCGTCGACCACCTCAGCAACAACTCGGCCACCAGCTACTTCGTCCTCGGCCCCCCCGCCGTCGCCTCCCGTGCCGCCACCTCCCTCCCCTCCGGCAACATGACCTTCACCGAGCTATTTATCGGCGAATTCTTCAGCAGCTACTACGGTGTGGGCCTCATCGGCATCTCCGCCGACGGGGTCATGCTCAACATCCCCCCTCGCGTTTGGGACATCACCGACGGCGGCGGCACCATCGTCGACTCCGGCACCAGTCTCACCATGCTGGCGGCGCCGGCCTTCGACATGGTAATGCAAGCCCTGACTCCGAAGCTCAAGCGATTCCCGAAACTCCAAATGGAACCCTTCACATTTTGCTTCAATAACAGCGAGTACTTGTACGAAATGACTCCGCAGCTGAGATTCCATTTCAGCGACGGCGCCGTGTTCGAGCCGCCGGTGAAGAGCTACATTATTACCGTCTCTGAATGGACTATGTGTGTTGCGGTGGTTTCTTTAGCTTTTCCGGCGACCAATATCATTGGCAATATTCTTCAGCAAAATCATCTTTGGCAATTTGATTTCTTCAAGAGAAGGATCGGCTTTGCTCCCTCCGACTGCGCCGCCTAGAATTTCATCTTCTTTTTCTTTCTTTTTATATTTTTAATTTAATGATTATTATTTTTCTCTTCTCTCTCTAAATCCTTGTTATTATTATTATTATTATTTTTTTTTTCTTTCGACAACTCCTTGTTATTATTACTTTGTTTTATATGTAATGCCATATTTTTATTTTTCTGTTTTTTTATGGTTCAGTTTTAATAAAGAAGAGCCGA

mRNA sequence

GCAGAGAGTGTAACAGATGGTGGAAGTTTGTTATAAAAACAGGGGAAGAAGAAACGCCATTATTAGGGCATTCTAAATTTACCACTTTTCTGTTGAGTTTCTGAATCTTCCTTCCCCTTTTTAAGTCCGCCATTGCCATCTTTCTCCTTGCTCCATCTCTCTAACATTGCAATATCTGTTTGTGTTTGTATGTTAGATTACAGGAAGCCATTAATTATGTCGCCTGTTTCAAATTTTTCGATCCTTTTCTTCTCCATCTTCTTCTTCCTCTCCGTTCACATTGCATTCGGCGACGGCAATGATGACCAAGAAGAGGCGGTCAGGCTGGAACTGCTGCACCGCCACCATCCACAAGTCTCCGAGAAGCTTCACGGCGAGGCGAAGCTTCAGGGAGCCAACGAACGCGTGAAGGATATTCACGATCACGACCACAATCGCCACCGTTTCATCTCGGAGTCGCTGAATCGGACTCAAACCGAGAAGAAATTGATGGCGGAGGGGCAGATGACGACGGAGCCTTCGTCGTCGGCAACGCCGATCGGGATGAAAATGATCTCAGGCGCCGATTATGGAGCCAGCGAGTACTTCGTGGAATTGAAAGTCGGAACGCCGCCGCAGAAGTGCATGTTGATCGCGGATACCGGAAGTGACCTAACCTGGGTAAAATGCAGATACCGGCGGTGCATGGGGAATTGCAGCGCCAATCCCGCTCATATGATTCGAAACGAAAAGAAAGAGAGATTCCCTAGGGCGTTTCATGCCAATCGATCTTCCTCTTTCCAGCCGATCAGTTGCGGCAAGAAATGTATGGATGATTATGCCACTCTCTTCGCCCTCCCGGATTGTAAGACCCCTACCAGCCCCTGTCGCTATGATTACGGCTACTCAGGTGGATCAAGTGCAAGGGGAATATTCGCAATCGAGACCCTAACGGTGGCCCTAACAAACGGAAAAGAAAAGCAACTCCACGACACTTTAATCGGCTGCACCGAATCAGCCACAGGCAGAGTCTTCAACGGAGCCGACGGCGTCCTTGGCTTAGGGACTACCGTATTCTCCTTCCCCTACAGAGCCAGACAAAACGCCAACGGCGGCGGCTTCTCCTACTGCCTCGTCGACCACCTCAGCAACAACTCGGCCACCAGCTACTTCGTCCTCGGCCCCCCCGCCGTCGCCTCCCGTGCCGCCACCTCCCTCCCCTCCGGCAACATGACCTTCACCGAGCTATTTATCGGCGAATTCTTCAGCAGCTACTACGGTGTGGGCCTCATCGGCATCTCCGCCGACGGGGTCATGCTCAACATCCCCCCTCGCGTTTGGGACATCACCGACGGCGGCGGCACCATCGTCGACTCCGGCACCAGTCTCACCATGCTGGCGGCGCCGGCCTTCGACATGGTAATGCAAGCCCTGACTCCGAAGCTCAAGCGATTCCCGAAACTCCAAATGGAACCCTTCACATTTTGCTTCAATAACAGCGAGTACTTGTACGAAATGACTCCGCAGCTGAGATTCCATTTCAGCGACGGCGCCGTGTTCGAGCCGCCGGTGAAGAGCTACATTATTACCGTCTCTGAATGGACTATGTGTGTTGCGGTGGTTTCTTTAGCTTTTCCGGCGACCAATATCATTGGCAATATTCTTCAGCAAAATCATCTTTGGCAATTTGATTTCTTCAAGAGAAGGATCGGCTTTGCTCCCTCCGACTGCGCCGCCTAGAATTTCATCTTCTTTTTCTTTCTTTTTATATTTTTAATTTAATGATTATTATTTTTCTCTTCTCTCTCTAAATCCTTGTTATTATTATTATTATTATTTTTTTTTTCTTTCGACAACTCCTTGTTATTATTACTTTGTTTTATATGTAATGCCATATTTTTATTTTTCTGTTTTTTTATGGTTCAGTTTTAATAAAGAAGAGCCGA

Coding sequence (CDS)

ATGTTAGATTACAGGAAGCCATTAATTATGTCGCCTGTTTCAAATTTTTCGATCCTTTTCTTCTCCATCTTCTTCTTCCTCTCCGTTCACATTGCATTCGGCGACGGCAATGATGACCAAGAAGAGGCGGTCAGGCTGGAACTGCTGCACCGCCACCATCCACAAGTCTCCGAGAAGCTTCACGGCGAGGCGAAGCTTCAGGGAGCCAACGAACGCGTGAAGGATATTCACGATCACGACCACAATCGCCACCGTTTCATCTCGGAGTCGCTGAATCGGACTCAAACCGAGAAGAAATTGATGGCGGAGGGGCAGATGACGACGGAGCCTTCGTCGTCGGCAACGCCGATCGGGATGAAAATGATCTCAGGCGCCGATTATGGAGCCAGCGAGTACTTCGTGGAATTGAAAGTCGGAACGCCGCCGCAGAAGTGCATGTTGATCGCGGATACCGGAAGTGACCTAACCTGGGTAAAATGCAGATACCGGCGGTGCATGGGGAATTGCAGCGCCAATCCCGCTCATATGATTCGAAACGAAAAGAAAGAGAGATTCCCTAGGGCGTTTCATGCCAATCGATCTTCCTCTTTCCAGCCGATCAGTTGCGGCAAGAAATGTATGGATGATTATGCCACTCTCTTCGCCCTCCCGGATTGTAAGACCCCTACCAGCCCCTGTCGCTATGATTACGGCTACTCAGGTGGATCAAGTGCAAGGGGAATATTCGCAATCGAGACCCTAACGGTGGCCCTAACAAACGGAAAAGAAAAGCAACTCCACGACACTTTAATCGGCTGCACCGAATCAGCCACAGGCAGAGTCTTCAACGGAGCCGACGGCGTCCTTGGCTTAGGGACTACCGTATTCTCCTTCCCCTACAGAGCCAGACAAAACGCCAACGGCGGCGGCTTCTCCTACTGCCTCGTCGACCACCTCAGCAACAACTCGGCCACCAGCTACTTCGTCCTCGGCCCCCCCGCCGTCGCCTCCCGTGCCGCCACCTCCCTCCCCTCCGGCAACATGACCTTCACCGAGCTATTTATCGGCGAATTCTTCAGCAGCTACTACGGTGTGGGCCTCATCGGCATCTCCGCCGACGGGGTCATGCTCAACATCCCCCCTCGCGTTTGGGACATCACCGACGGCGGCGGCACCATCGTCGACTCCGGCACCAGTCTCACCATGCTGGCGGCGCCGGCCTTCGACATGGTAATGCAAGCCCTGACTCCGAAGCTCAAGCGATTCCCGAAACTCCAAATGGAACCCTTCACATTTTGCTTCAATAACAGCGAGTACTTGTACGAAATGACTCCGCAGCTGAGATTCCATTTCAGCGACGGCGCCGTGTTCGAGCCGCCGGTGAAGAGCTACATTATTACCGTCTCTGAATGGACTATGTGTGTTGCGGTGGTTTCTTTAGCTTTTCCGGCGACCAATATCATTGGCAATATTCTTCAGCAAAATCATCTTTGGCAATTTGATTTCTTCAAGAGAAGGATCGGCTTTGCTCCCTCCGACTGCGCCGCCTAG

Protein sequence

MLDYRKPLIMSPVSNFSILFFSIFFFLSVHIAFGDGNDDQEEAVRLELLHRHHPQVSEKLHGEAKLQGANERVKDIHDHDHNRHRFISESLNRTQTEKKLMAEGQMTTEPSSSATPIGMKMISGADYGASEYFVELKVGTPPQKCMLIADTGSDLTWVKCRYRRCMGNCSANPAHMIRNEKKERFPRAFHANRSSSFQPISCGKKCMDDYATLFALPDCKTPTSPCRYDYGYSGGSSARGIFAIETLTVALTNGKEKQLHDTLIGCTESATGRVFNGADGVLGLGTTVFSFPYRARQNANGGGFSYCLVDHLSNNSATSYFVLGPPAVASRAATSLPSGNMTFTELFIGEFFSSYYGVGLIGISADGVMLNIPPRVWDITDGGGTIVDSGTSLTMLAAPAFDMVMQALTPKLKRFPKLQMEPFTFCFNNSEYLYEMTPQLRFHFSDGAVFEPPVKSYIITVSEWTMCVAVVSLAFPATNIIGNILQQNHLWQFDFFKRRIGFAPSDCAA

Homology

[Close]

BLAST of Lcy07g014030 vs. ExPASy Swiss-Prot
Match: Q9LTW4 (Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE=1 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 2.1e-86
Identity = 192/474 (40.51%), Postives = 258/474 (54.43%), Query Frame = 0


Query: 40  QEEAVRLELLHRHHPQVSEKLHGEAKLQGANERVKDIHDHDHNRHRFISESLNRTQTEKK 99
           ++ +VRL+L HR           +  L     R++D+   D  RH  IS   N T     
Sbjct: 45  KDTSVRLKLAHR-----------DTLLPKPLSRIEDVIGADQKRHSLISRKRNST----- 104

Query: 100 LMAEGQMTTEPSSSATPIGMKMI--SGADYGASEYFVELKVGTPPQKCMLIADTGSDLTW 159
                            +G+KM   SG DYG ++YF E++VGTP +K  ++ DTGS+LTW
Sbjct: 105 -----------------VGVKMDLGSGIDYGTAQYFTEIRVGTPAKKFRVVVDTGSELTW 164

Query: 160 VKCRYRRCMGNCSANPAHMIRNEKKERFPRAFHANRSSSFQPISC-GKKCMDDYATLFAL 219
           V CRYR                 K  R  R F A+ S SF+ + C  + C  D   LF+L
Sbjct: 165 VNCRYR--------------ARGKDNR--RVFRADESKSFKTVGCLTQTCKVDLMNLFSL 224

Query: 220 PDCKTPTSPCRYDYGYSGGSSARGIFAIETLTVALTNGKEKQLHDTLIGCTESATGRVFN 279
             C TP++PC YDY Y+ GS+A+G+FA ET+TV LTNG+  +L   LIGC+ S TG+ F 
Sbjct: 225 TTCPTPSTPCSYDYRYADGSAAQGVFAKETITVGLTNGRMARLPGHLIGCSSSFTGQSFQ 284

Query: 280 GADGVLGLGTTVFSFPYRARQNANGGGFSYCLVDHLSNNSATSYFVLGPPAVASRAATSL 339
           GADGVLGL  + FSF   A  +  G  FSYCLVDHLSN + ++Y + G     S  +T  
Sbjct: 285 GADGVLGLAFSDFSFTSTA-TSLYGAKFSYCLVDHLSNKNVSNYLIFG-----SSRSTKT 344

Query: 340 PSGNMTFTELFIGEFFSSYYGVGLIGISADGVMLNIPPRVWDITDGGGTIVDSGTSLTML 399
                T  +L        +Y + +IGIS    ML+IP +VWD T GGGTI+DSGTSLT+L
Sbjct: 345 AFRRTTPLDL---TRIPPFYAINVIGISLGYDMLDIPSQVWDATSGGGTILDSGTSLTLL 404

Query: 400 AAPAFDMVMQALTPKLKRFPKLQME--PFTFCFN-NSEYLYEMTPQLRFHFSDGAVFEPP 459
           A  A+  V+  L   L    +++ E  P  +CF+  S +     PQL FH   GA FEP 
Sbjct: 405 ADAAYKQVVTGLARYLVELKRVKPEGVPIEYCFSFTSGFNVSKLPQLTFHLKGGARFEPH 460

Query: 460 VKSYIITVSEWTMCVAVVSLAFPATNIIGNILQQNHLWQFDFFKRRIGFAPSDC 508
            KSY++  +    C+  VS   PATN+IGNI+QQN+LW+FD     + FAPS C
Sbjct: 465 RKSYLVDAAPGVKCLGFVSAGTPATNVIGNIMQQNYLWEFDLMASTLSFAPSAC 460

[Close]

BLAST of Lcy07g014030 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 157.9 bits (398), Expect = 3.0e-37
Identity = 120/435 (27.59%), Postives = 199/435 (45.75%), Query Frame = 0


Query: 78  DHDHNRHRFISESLNRTQT-EKKLMAEGQMTTEPSSSATPIGMKMISGADYGASEYFVEL 137
           +H H  H  +    +R     +++  +   +++        G  ++SG D G+ EYFV +
Sbjct: 76  NHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEVNDFGSDIVSGMDQGSGEYFVRI 135

Query: 138 KVGTPPQKCMLIADTGSDLTWVKCRYRRCMGNCSANPAHMIRNEKKERFPRAFHANRSSS 197
            VG+PP+   ++ D+GSD+ WV+C+           P  +   +    F  A    +S S
Sbjct: 136 GVGSPPRDQYMVIDSGSDMVWVQCQ-----------PCKLCYKQSDPVFDPA----KSGS 195

Query: 198 FQPISCGKKCMDDYATLFALPDCKTPTSPCRYDYGYSGGSSARGIFAIETLTVALTNGKE 257
           +  +SCG    D       + +    +  CRY+  Y  GS  +G  A+ETLT A T    
Sbjct: 196 YTGVSCGSSVCD------RIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFAKT---- 255

Query: 258 KQLHDTLIGCTESATGRVFNGADGVLGLGTTVFSFPYRARQNANGGGFSYCLVDHLSNNS 317
             + +  +GC     G +F GA G+LG+G    SF  +      GG F YCLV       
Sbjct: 256 -VVRNVAMGCGHRNRG-MFIGAAGLLGIGGGSMSFVGQL-SGQTGGAFGYCLVSR--GTD 315

Query: 318 ATSYFVLGPPAVASRAATSLPSGNMTFTELFIGEFFSSYYGVGLIGISADGVMLNIPPRV 377
           +T   V G  A        LP G  ++  L       S+Y VGL G+   GV + +P  V
Sbjct: 316 STGSLVFGREA--------LPVG-ASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLPDGV 375

Query: 378 WDITD--GGGTIVDSGTSLTMLAAPAFDMVMQALTPKLKRFPKLQ-MEPFTFCFNNSEYL 437
           +D+T+   GG ++D+GT++T L   A+         +    P+   +  F  C++ S ++
Sbjct: 376 FDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFV 435

Query: 438 YEMTPQLRFHFSDGAVFEPPVKSYIITVSE-WTMCVAVVSLAFPATNIIGNILQQNHLWQ 497
               P + F+F++G V   P +++++ V +  T C A  + +    +IIGNI Q+     
Sbjct: 436 SVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAA-SPTGLSIIGNIQQEGIQVS 470

Query: 498 FDFFKRRIGFAPSDC 508
           FD     +GF P+ C
Sbjct: 496 FDGANGFVGFGPNVC 470

[Close]

BLAST of Lcy07g014030 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 1.9e-36
Identity = 130/396 (32.83%), Postives = 174/396 (43.94%), Query Frame = 0


Query: 121 MISGADYGASEYFVELKVGTPPQKCMLIADTGSDLTWVKCR-YRRCMGNCSANPAHMIRN 180
           ++SG   G+ EYF  L VGTP +   ++ DTGSD+ W++C   RRC     ++P      
Sbjct: 131 VVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYS--QSDP------ 190

Query: 181 EKKERFPRAFHANRSSSFQPISCGKKCMDDYATLFALPDCKTPTSPCRYDYGYSGGSSAR 240
                    F   +S ++  I C       +        C T    C Y   Y  GS   
Sbjct: 191 --------IFDPRKSKTYATIPCS----SPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTV 250

Query: 241 GIFAIETLTVALTNGKEKQLHDTLIGCTESATGRVFNGADGVLGLGTTVFSFPYRARQNA 300
           G F+ ETLT      +  ++    +GC     G +F GA G+LGLG    SFP +     
Sbjct: 251 GDFSTETLTF-----RRNRVKGVALGCGHDNEG-LFVGAAGLLGLGKGKLSFPGQTGHRF 310

Query: 301 NGGGFSYCLVDHLSNNSATSYFVLGPPAVASRAATSLPSGNMTFTELFIGEFFSSYYGVG 360
           N   FSYCLVD  S +S  S  V G  AV SR A         FT L       ++Y VG
Sbjct: 311 N-QKFSYCLVDR-SASSKPSSVVFGNAAV-SRIA--------RFTPLLSNPKLDTFYYVG 370

Query: 361 LIGISADGVML-NIPPRVW--DITDGGGTIVDSGTSLTMLAAPAFDMVMQAL---TPKLK 420
           L+GIS  G  +  +   ++  D    GG I+DSGTS+T L  PA+  +  A       LK
Sbjct: 371 LLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLK 430

Query: 421 RFPKLQMEPFTFCFNNSEYLYEMTPQLRFHFSDGAVFEPPVKSYIITV-SEWTMCVAVVS 480
           R P   +  F  CF+ S       P +  HF  GA    P  +Y+I V +    C A   
Sbjct: 431 RAPDFSL--FDTCFDLSNMNEVKVPTVVLHFR-GADVSLPATNYLIPVDTNGKFCFAFAG 485

Query: 481 LAFPATNIIGNILQQNHLWQFDFFKRRIGFAPSDCA 509
                 +IIGNI QQ     +D    R+GFAP  CA
Sbjct: 491 -TMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

[Close]

BLAST of Lcy07g014030 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 2.8e-35
Identity = 121/429 (28.21%), Postives = 199/429 (46.39%), Query Frame = 0


Query: 85  RFISESLNRTQTEKKLMAEGQMTTEPSSSATPIGMKMISGADYGASEYFVELKVGTPPQK 144
           RF  E ++R+  +     + +  TE     TP+    +SGA  G+ EYF  + VGTP ++
Sbjct: 121 RFAVEGVDRSDLKPVYNEDTRYQTE--DLTTPV----VSGASQGSGEYFSRIGVGTPAKE 180

Query: 145 CMLIADTGSDLTWVKCRYRRCMGNCSANPAHMIRNEKKERFPRAFHANRSSSFQPISCGK 204
             L+ DTGSD+ W++C        C+         +  ++    F+   SS+++ ++C  
Sbjct: 181 MYLVLDTGSDVNWIQCE------PCA---------DCYQQSDPVFNPTSSSTYKSLTCSA 240

Query: 205 KCMDDYATLFALPDCKTPTSPCRYDYGYSGGSSARGIFAIETLTVALTNGKEKQLHDTLI 264
                  +L     C+  ++ C Y   Y  GS   G  A +T+T     G   ++++  +
Sbjct: 241 ----PQCSLLETSACR--SNKCLYQVSYGDGSFTVGELATDTVTF----GNSGKINNVAL 300

Query: 265 GCTESATGRVFNGADGVLGLGTTVFSFPYRARQNANGGGFSYCLVDHLSNNSATSYFVLG 324
           GC     G +F GA G+LGLG  V S   + +  +    FSYCLVD  S  S++  F   
Sbjct: 301 GCGHDNEG-LFTGAAGLLGLGGGVLSITNQMKATS----FSYCLVDRDSGKSSSLDF--- 360

Query: 325 PPAVASRAATSLPSGNMTFTELFIGEFFSSYYGVGLIGISADGVMLNIPPRVWDI--TDG 384
                   +  L  G+ T   L   +   ++Y VGL G S  G  + +P  ++D+  +  
Sbjct: 361 -------NSVQLGGGDAT-APLLRNKKIDTFYYVGLSGFSVGGEKVVLPDAIFDVDASGS 420

Query: 385 GGTIVDSGTSLTMLAAPAFDMVMQA---LTPKLKRFPKLQMEPFTFCFNNSEYLYEMTPQ 444
           GG I+D GT++T L   A++ +  A   LT  LK+     +  F  C++ S       P 
Sbjct: 421 GGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKK-GSSSISLFDTCYDFSSLSTVKVPT 480

Query: 445 LRFHFSDGAVFEPPVKSYIITVSE-WTMCVAVVSLAFPATNIIGNILQQNHLWQFDFFKR 504
           + FHF+ G   + P K+Y+I V +  T C A    +  + +IIGN+ QQ     +D  K 
Sbjct: 481 VAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTS-SSLSIIGNVQQQGTRITYDLSKN 500

Query: 505 RIGFAPSDC 508
            IG + + C
Sbjct: 541 VIGLSGNKC 500

[Close]

BLAST of Lcy07g014030 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 3.9e-29
Identity = 104/384 (27.08%), Postives = 166/384 (43.23%), Query Frame = 0


Query: 129 ASEYFVELKVGTPPQKCMLIADTGSDLTWVKCRYRRCMGNCSANPAHMIRNEKKERFPRA 188
           + EY + + +GTPP   M IADTGSDL W +C        C         ++   +    
Sbjct: 87  SGEYLMNVSIGTPPFPIMAIADTGSDLLWTQC------APC---------DDCYTQVDPL 146

Query: 189 FHANRSSSFQPISCGKKCMDDYATLFALPDCKTPTSPCRYDYGYSGGSSARGIFAIETLT 248
           F    SS+++ +SC          L     C T  + C Y   Y   S  +G  A++TLT
Sbjct: 147 FDPKTSSTYKDVSCSS---SQCTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLT 206

Query: 249 VALTNGKEKQLHDTLIGCTESATGRVFNGADGVLGLGTTVFSFPYRARQNANGGGFSYCL 308
           +  ++ +  QL + +IGC  +  G       G++GLG    S   +   ++  G FSYCL
Sbjct: 207 LGSSDTRPMQLKNIIIGCGHNNAGTFNKKGSGIVGLGGGPVSL-IKQLGDSIDGKFSYCL 266

Query: 309 VDHLSNNSATSYFVLGPPAVASRAATSLPSGNMTFTELFIGEFFSSYYGVGLIGISADGV 368
           V   S    TS    G  A+ S          +  T L       ++Y + L  IS    
Sbjct: 267 VPLTSKKDQTSKINFGTNAIVS-------GSGVVSTPLIAKASQETFYYLTLKSISVGSK 326

Query: 369 MLNIPPRVWDITDGGGTIVDSGTSLTMLAAPAF----DMVMQALTPKLKRFPKLQMEPFT 428
            +       + ++ G  I+DSGT+LT+L    +    D V  ++  + K+ P+      +
Sbjct: 327 QIQYSGSDSESSE-GNIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQ---SGLS 386

Query: 429 FCFNNSEYLYEMTPQLRFHFSDGAVFEPPVKSYIITVSEWTMCVAVVSLAFPATNIIGNI 488
            C++ +  L    P +  HF DGA  +    +  + VSE  +C A      P+ +I GN+
Sbjct: 387 LCYSATGDL--KVPVITMHF-DGADVKLDSSNAFVQVSEDLVCFAF--RGSPSFSIYGNV 435

Query: 489 LQQNHLWQFDFFKRRIGFAPSDCA 509
            Q N L  +D   + + F P+DCA
Sbjct: 447 AQMNFLVGYDTVSKTVSFKPTDCA 435

[Close]

BLAST of Lcy07g014030 vs. ExPASy TrEMBL
Match: A0A0A0KG92 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G134390 PE=3 SV=1)

HSP 1 Score: 680.6 bits (1755), Expect = 4.9e-192
Identity = 337/539 (62.52%), Postives = 419/539 (77.74%), Query Frame = 0


Query: 1   MLDYRKPLIMSPVSNFS--ILFFSIFFFLSVHIAF----GD------------GNDDQEE 60
           ML YRKP  MSP+SNF     FF +FFFLS   +F    GD             ++D++E
Sbjct: 1   MLGYRKP--MSPISNFCFFFFFFLLFFFLSFSSSFLFALGDEDNNFNNNNNINDDEDEQE 60

Query: 61  AVRLELLHRHHPQVSEKLHGEAKLQGANERVKDIHDHDHNRHRFISESLNRTQTE-KKLM 120
            ++ +LLHRHHPQV+EK+HG+ K+Q  +ER+KDIH+HDHNRHR IS+S+N+ Q E  +L 
Sbjct: 61  IIKFDLLHRHHPQVAEKIHGDMKIQDVSERMKDIHEHDHNRHRSISKSMNQKQVEDARLR 120

Query: 121 AEGQMTTE---------PSSSATPIGMKMISGADYGASEYFVELKVGTPPQKCMLIADTG 180
           AE +  TE         P +++TPIGM+MISGAD+G+SEYFVELKVGTP Q  MLIADTG
Sbjct: 121 AEAEAATEEEVAKSAILPPATSTPIGMRMISGADFGSSEYFVELKVGTPAQTFMLIADTG 180

Query: 181 SDLTWVKCRYRRCMGNCSANPAHMIRNEKKERFPRAFHANRSSSFQPISCGK-KCMDDYA 240
           SDLTW+KCRYRRC GNCS+N  H  +NEKK+RF  AF AN SSSF+ +SC    C +D A
Sbjct: 181 SDLTWMKCRYRRCFGNCSSNVNHKSKNEKKQRFRHAFLANHSSSFKTVSCSSTMCTNDLA 240

Query: 241 TLFALPDCKTPTSPCRYDYGYSGGSSARGIFAIETLTVALTNGKEKQLHDTLIGCTESAT 300
            LFA+ +C  PTSPC YDY Y+GG+SA+GIFA ETLTV LTNGKEKQLH+++IGCTES  
Sbjct: 241 DLFAVRECHNPTSPCVYDYSYTGGASAKGIFAWETLTVGLTNGKEKQLHNSIIGCTESVQ 300

Query: 301 GRVFNGADGVLGLGTTVFSFPYRARQNANGGGFSYCLVDHLSNNSATSYFVLGPPAVASR 360
           G VF GADGV+GLGT+ +S  Y+A +NANGGGFSYCLVDHL++  A SYFVLG P  ++ 
Sbjct: 301 GSVFGGADGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAISYFVLGIPTPSTS 360

Query: 361 AATS---LPSGNMTFTELFIGEFFSSYYGVGLIGISADGVMLNIPPRVWDITDGGGTIVD 420
           A+TS   LP+  MT+T+L++G+ +SS+YGV LIGISA+G+MLNIP RVWDI  GGGTI+D
Sbjct: 361 ASTSSAKLPA-KMTYTKLYVGDPYSSFYGVDLIGISANGIMLNIPSRVWDINSGGGTIID 420

Query: 421 SGTSLTMLAAPAFDMVMQALTPKLKRFPKLQMEPFTFCFNNSEYLYEMTPQLRFHFSDGA 480
           SGTSLT+LAAPAFDMVM+ALTP+LK+F +L++EPF FCFNNS+Y +EM P+LRFHF DG 
Sbjct: 421 SGTSLTILAAPAFDMVMEALTPRLKKFQQLEIEPFDFCFNNSQYTHEMAPKLRFHFGDGT 480

Query: 481 VFEPPVKSYIITVSEWTMCVAVVSLAFPATNIIGNILQQNHLWQFDFFKRRIGFAPSDC 508
           VFEPP KSYI++V ++  C+  VS+ FPA NIIGNILQQNHLWQFDF KRR+GFAPS+C
Sbjct: 481 VFEPPTKSYIVSVGKFISCIGFVSMPFPANNIIGNILQQNHLWQFDFQKRRVGFAPSEC 536

[Close]

BLAST of Lcy07g014030 vs. ExPASy TrEMBL
Match: A0A1S3C2F3 (aspartic proteinase CDR1 OS=Cucumis melo OX=3656 GN=LOC103496268 PE=3 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 6.4e-184
Identity = 325/532 (61.09%), Postives = 409/532 (76.88%), Query Frame = 0


Query: 1   MLDYRKPLIMSPVSNFSILFFSIFFFLSVHIAF----GD------GNDDQEE--AVRLEL 60
           ML YRKP  MSP+SNF   FF + FFLS   +F    GD       NDD++E   +R +L
Sbjct: 1   MLGYRKP--MSPISNF-CFFFLLLFFLSFSSSFLFALGDEANNYNNNDDEDEQQTIRFDL 60

Query: 61  LHRHHPQVSEKLHGEAKLQGANERVKDIHDHDHNRHRFISESLNRTQTE-KKLMAEGQMT 120
           LHRHHPQVSEKL+G+ K+Q  +ER+KDIH+HD NRHR IS+S+N+ Q E  +L AE +  
Sbjct: 61  LHRHHPQVSEKLNGDMKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAA 120

Query: 121 TE---------PSSSATPIGMKMISGADYGASEYFVELKVGTPPQKCMLIADTGSDLTWV 180
           T+         P +++TPIGMKMISGAD+G+SEYFV+LKVGTP Q  MLIADTGSDLTW+
Sbjct: 121 TQVEVAKSAILPPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWL 180

Query: 181 KCRYRRCMGNCSANPAHMIRNEKKERFPRAFHANRSSSFQPISCGK-KCMDDYATLFALP 240
           KCRYRRC GNCS N  H  +NEKK+RF  A  AN+SS+F+ +SC    C ++ A LFA+ 
Sbjct: 181 KCRYRRCFGNCSGNVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVA 240

Query: 241 DCKTPTSPCRYDYGYSGGSSARGIFAIETLTVALTNGKEKQLHDTLIGCTESATGRVFNG 300
           +C TPTSPC YDY Y+GG+SA+GIFA ETLTV LTNGKEKQL +++IGCTE   G VF+G
Sbjct: 241 ECDTPTSPCVYDYSYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDG 300

Query: 301 ADGVLGLGTTVFSFPYRARQNANGGGFSYCLVDHLSNNSATSYFVLGPPAVASRAATS-- 360
           ADGV+GLGT+ +S  Y+A +NANGGGFSYCLVDHL++  A SYFVLG P  ++ A+TS  
Sbjct: 301 ADGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSA 360

Query: 361 LPSGNMTFTELFIGEFFSSYYGVGLIGISADGVMLNIPPRVWDITDGGGTIVDSGTSLTM 420
            P   M++T+L++G+ +SS+YGV LIGISADG MLNIPPRVWD   G GTI+DSGTSLT+
Sbjct: 361 KPPAKMSYTKLYVGDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTV 420

Query: 421 LAAPAFDMVMQALTPKLKRFPKLQMEPFTFCFNNSEYLYEMTPQLRFHFSDGAVFEPPVK 480
           LA PAFD+VM+ LT +LK+F ++++EPF FCFNNS+Y ++M P+LRFHF DG VFEPP K
Sbjct: 421 LATPAFDVVMEVLTSRLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTK 480

Query: 481 SYIITVSEWTMCVAVVSLAFPATNIIGNILQQNHLWQFDFFKRRIGFAPSDC 508
           SYI++V E+  C+ +VS+ FP+ NIIGNILQQNHLWQFDF KRR+GFA S+C
Sbjct: 481 SYIVSVGEFISCIGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASEC 529

[Close]

BLAST of Lcy07g014030 vs. ExPASy TrEMBL
Match: A0A5D3B701 (Aspartic proteinase CDR1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold104G00400 PE=3 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 3.6e-166
Identity = 285/456 (62.50%), Postives = 360/456 (78.95%), Query Frame = 0


Query: 65  KLQGANERVKDIHDHDHNRHRFISESLNRTQTE-KKLMAEGQMTTE---------PSSSA 124
           K+Q  +ER+KDIH+HD NRHR IS+S+N+ Q E  +L AE +  T+         P +++
Sbjct: 2   KIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQVEVAKSAILPPATS 61

Query: 125 TPIGMKMISGADYGASEYFVELKVGTPPQKCMLIADTGSDLTWVKCRYRRCMGNCSANPA 184
           TPIGMKMISGAD+G+SEYFV+LKVGTP Q  MLIADTGSDLTW+KCRYRRC GNCS N  
Sbjct: 62  TPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCRYRRCFGNCSGNVN 121

Query: 185 HMIRNEKKERFPRAFHANRSSSFQPISCGK-KCMDDYATLFALPDCKTPTSPCRYDYGYS 244
           H  +NEKK+RF  A  AN+SS+F+ +SC    C ++ A LFA+ +C TPTSPC YDY Y+
Sbjct: 122 HKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECDTPTSPCVYDYSYA 181

Query: 245 GGSSARGIFAIETLTVALTNGKEKQLHDTLIGCTESATGRVFNGADGVLGLGTTVFSFPY 304
           GG+SA+GIFA ETLTV LTNGKEKQL +++IGCTE   G VF+GADGV+GLGT+ +S  Y
Sbjct: 182 GGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADGVMGLGTSSYSLTY 241

Query: 305 RARQNANGGGFSYCLVDHLSNNSATSYFVLGPPAVASRAATS--LPSGNMTFTELFIGEF 364
           +A +NANGGGFSYCLVDHL++  A SYFVLG P  ++ A+TS   P   M++T+L++G+ 
Sbjct: 242 KAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYTKLYVGDP 301

Query: 365 FSSYYGVGLIGISADGVMLNIPPRVWDITDGGGTIVDSGTSLTMLAAPAFDMVMQALTPK 424
           +SS+YGV LIGISADG MLNIPPRVWD   G GTI+DSGTSLT+LA PAFD+VM+ LT +
Sbjct: 302 YSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLATPAFDVVMEVLTSR 361

Query: 425 LKRFPKLQMEPFTFCFNNSEYLYEMTPQLRFHFSDGAVFEPPVKSYIITVSEWTMCVAVV 484
           LK+F ++++EPF FCFNNS+Y ++M P+LRFHF DG VFEPP KSYI++V E+  C+ +V
Sbjct: 362 LKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYIVSVGEFISCIGIV 421

Query: 485 SLAFPATNIIGNILQQNHLWQFDFFKRRIGFAPSDC 508
           S+ FP+ NIIGNILQQNHLWQFDF KRR+GFA S+C
Sbjct: 422 SMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASEC 457

[Close]

BLAST of Lcy07g014030 vs. ExPASy TrEMBL
Match: A0A6J1FXD5 (aspartic proteinase NANA, chloroplast-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448433 PE=3 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 6.1e-166
Identity = 296/516 (57.36%), Postives = 373/516 (72.29%), Query Frame = 0


Query: 1   MLDYRKPLIMSPVSNFSILFFSIFFFLSVHIAFGDGNDDQEE-------AVRLELLHRHH 60
           ML Y  P  MSP+S   ++FF   FFLSVH+AF DG++ Q++        V+L+++HRHH
Sbjct: 1   MLGYTNP--MSPISPL-LIFFFFNFFLSVHVAF-DGDEQQQQQKPSEMPMVKLDVMHRHH 60

Query: 61  PQVSEKLHGEAKLQGANERVKDIHDHDHNRHRFISESLNRTQTEKKLMAEGQMTTEPSSS 120
           P V EKL+GE +  G+ +R +DIH+HDHNR R IS S+  ++T+++L         P  S
Sbjct: 61  PHVQEKLYGERRSLGSTDRFRDIHEHDHNRQRSISTSMKMSKTDRQL---------PMPS 120

Query: 121 ATPIGMKMISGADYGASEYFVELKVGTPPQKCMLIADTGSDLTWVKCRYRRCMGNCSANP 180
           + PI +K+ SG D+G +EYFV+ +VGTPPQK +LI DTGSDLTW+KCRYRRC+GNC+A+ 
Sbjct: 121 SAPIQLKISSGFDFGTNEYFVQFRVGTPPQKFLLIVDTGSDLTWLKCRYRRCLGNCTAHA 180

Query: 181 AHMIRNEKKERFPRAFHANRSSSFQPISCGKK-CMDDYATLFALPDCKTPTSPCRYDYGY 240
            H  R E K +F   F AN SSSF+ I+CG   C+ D   LFA+PDC+ P++PC YDY Y
Sbjct: 181 HHKSRVEHKVKFDHPFLANHSSSFKLITCGSDFCLGDLQLLFAIPDCQVPSNPCVYDYSY 240

Query: 241 SGGSSARGIFAIETLTVALTNGKEKQLHDTLIGCTESATGRVFNGADGVLGLGTTVFSFP 300
            GG +A G+FA ET+TV LTNGKEKQLHDTLIGCTE        G DG+LGLGT   SF 
Sbjct: 241 IGGGAATGLFANETVTVGLTNGKEKQLHDTLIGCTELFNAMQLKGVDGILGLGTGAHSFA 300

Query: 301 YRARQNANGGGFSYCLVDHLSNNSATSYFVLG-PPAVASRAATSLPSGNMTFTELFIGEF 360
           +RA  + NGGGFSYCL+DHLS++SATSYF+LG PPA     A   P GNMTF  L +G  
Sbjct: 301 HRAALDKNGGGFSYCLIDHLSHHSATSYFILGYPPAEPLSVA---PVGNMTFINLHLGGP 360

Query: 361 FSSYYGVGLIGISADGVMLNIPPRVWDITDGGGTIVDSGTSLTMLAAPAFDMVMQALTPK 420
           F+SYYGVGLIGIS DGV LNIPPRVWDI  GGGTI+DSGTSL+ML APAFD+ M+A+  K
Sbjct: 361 FNSYYGVGLIGISIDGVTLNIPPRVWDIQKGGGTILDSGTSLSMLTAPAFDVFMEAMVQK 420

Query: 421 LKRFPKLQMEPFTFCFNNSEYLYEMTPQLRFHFSDGAVFEPPVKSYIITVSEWTMCVAVV 480
           LK+F ++  +PF +CFN + Y +EM P+LRFHF  G VFEPP KSYI+ V +  +C+   
Sbjct: 421 LKKFQQILADPFAYCFNKTHYSHEMAPKLRFHFEKGVVFEPPPKSYIVKVDD-ILCLGFT 480

Query: 481 SLAFPATNIIGNILQQNHLWQFDFFKRRIGFAPSDC 508
           S+ FP TNIIGNILQQN LWQFDFF +++GFAPS C
Sbjct: 481 SIPFPDTNIIGNILQQNFLWQFDFFNKKVGFAPSQC 499

[Close]

BLAST of Lcy07g014030 vs. ExPASy TrEMBL
Match: A0A6J1FVB3 (aspartic proteinase NANA, chloroplast-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448433 PE=3 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 8.8e-165
Identity = 292/507 (57.59%), Postives = 369/507 (72.78%), Query Frame = 0


Query: 10  MSPVSNFSILFFSIFFFLSVHIAFGDGNDDQEE-------AVRLELLHRHHPQVSEKLHG 69
           MSP+S   ++FF   FFLSVH+AF DG++ Q++        V+L+++HRHHP V EKL+G
Sbjct: 1   MSPISPL-LIFFFFNFFLSVHVAF-DGDEQQQQQKPSEMPMVKLDVMHRHHPHVQEKLYG 60

Query: 70  EAKLQGANERVKDIHDHDHNRHRFISESLNRTQTEKKLMAEGQMTTEPSSSATPIGMKMI 129
           E +  G+ +R +DIH+HDHNR R IS S+  ++T+++L         P  S+ PI +K+ 
Sbjct: 61  ERRSLGSTDRFRDIHEHDHNRQRSISTSMKMSKTDRQL---------PMPSSAPIQLKIS 120

Query: 130 SGADYGASEYFVELKVGTPPQKCMLIADTGSDLTWVKCRYRRCMGNCSANPAHMIRNEKK 189
           SG D+G +EYFV+ +VGTPPQK +LI DTGSDLTW+KCRYRRC+GNC+A+  H  R E K
Sbjct: 121 SGFDFGTNEYFVQFRVGTPPQKFLLIVDTGSDLTWLKCRYRRCLGNCTAHAHHKSRVEHK 180

Query: 190 ERFPRAFHANRSSSFQPISCGKK-CMDDYATLFALPDCKTPTSPCRYDYGYSGGSSARGI 249
            +F   F AN SSSF+ I+CG   C+ D   LFA+PDC+ P++PC YDY Y GG +A G+
Sbjct: 181 VKFDHPFLANHSSSFKLITCGSDFCLGDLQLLFAIPDCQVPSNPCVYDYSYIGGGAATGL 240

Query: 250 FAIETLTVALTNGKEKQLHDTLIGCTESATGRVFNGADGVLGLGTTVFSFPYRARQNANG 309
           FA ET+TV LTNGKEKQLHDTLIGCTE        G DG+LGLGT   SF +RA  + NG
Sbjct: 241 FANETVTVGLTNGKEKQLHDTLIGCTELFNAMQLKGVDGILGLGTGAHSFAHRAALDKNG 300

Query: 310 GGFSYCLVDHLSNNSATSYFVLG-PPAVASRAATSLPSGNMTFTELFIGEFFSSYYGVGL 369
           GGFSYCL+DHLS++SATSYF+LG PPA     A   P GNMTF  L +G  F+SYYGVGL
Sbjct: 301 GGFSYCLIDHLSHHSATSYFILGYPPAEPLSVA---PVGNMTFINLHLGGPFNSYYGVGL 360

Query: 370 IGISADGVMLNIPPRVWDITDGGGTIVDSGTSLTMLAAPAFDMVMQALTPKLKRFPKLQM 429
           IGIS DGV LNIPPRVWDI  GGGTI+DSGTSL+ML APAFD+ M+A+  KLK+F ++  
Sbjct: 361 IGISIDGVTLNIPPRVWDIQKGGGTILDSGTSLSMLTAPAFDVFMEAMVQKLKKFQQILA 420

Query: 430 EPFTFCFNNSEYLYEMTPQLRFHFSDGAVFEPPVKSYIITVSEWTMCVAVVSLAFPATNI 489
           +PF +CFN + Y +EM P+LRFHF  G VFEPP KSYI+ V +  +C+   S+ FP TNI
Sbjct: 421 DPFAYCFNKTHYSHEMAPKLRFHFEKGVVFEPPPKSYIVKVDD-ILCLGFTSIPFPDTNI 480

Query: 490 IGNILQQNHLWQFDFFKRRIGFAPSDC 508
           IGNILQQN LWQFDFF +++GFAPS C
Sbjct: 481 IGNILQQNFLWQFDFFNKKVGFAPSQC 492

[Close]

BLAST of Lcy07g014030 vs. NCBI nr
Match: XP_038901983.1 (aspartic proteinase NANA, chloroplast [Benincasa hispida])

HSP 1 Score: 706.8 bits (1823), Expect = 1.3e-199
Identity = 343/517 (66.34%), Postives = 418/517 (80.85%), Query Frame = 0


Query: 1   MLDYRKPLIMSPVSNFSILFFSIFFFLSVHIAFGDGNDDQEEAVRLELLHRHHPQVSEKL 60
           ML YRKP  MSP+S+F + F  +FFFLSV IAFGDG+ DQE  V+L+LLHRHHPQVSEKL
Sbjct: 1   MLGYRKP--MSPISHFCLFF--LFFFLSVPIAFGDGSHDQEN-VKLDLLHRHHPQVSEKL 60

Query: 61  HGEAKLQGANERVKDIHDHDHNRHRFISESLNRTQTEKKLMAEGQMTTE-----PSSSAT 120
           HG+ KL+  N+R+KDI +HD  R++ IS SLNR + +++L  E     E     P  S+T
Sbjct: 61  HGDIKLENMNDRIKDILEHDQKRYQTISSSLNRNELDEQLRKEAAELAEKDLKLPPISST 120

Query: 121 PIGMKMISGADYGASEYFVELKVGTPPQKCMLIADTGSDLTWVKCRYRRCMGNCSANPAH 180
           PIG+KMISG+DYG+SEYFV+LKVGTPPQ  MLIADTGSDLTW+KCRYRRC+GNCS+NP H
Sbjct: 121 PIGLKMISGSDYGSSEYFVQLKVGTPPQTFMLIADTGSDLTWMKCRYRRCIGNCSSNPNH 180

Query: 181 MIRNEKKERFPRAFHANRSSSFQPISCGKK-CMDDYATLFALPDCKTPTSPCRYDYGYSG 240
             RNE+K RF  AF AN SSSF+ I C  K C +D A LF++ +C+TPTSPC YDY YSG
Sbjct: 181 KTRNERKVRFRNAFLANYSSSFKTIDCSSKMCTNDLADLFSIGECQTPTSPCLYDYSYSG 240

Query: 241 GSSARGIFAIETLTVALTNGKEKQLHDTLIGCTESATGRVFNGADGVLGLGTTVFSFPYR 300
           G+SA+G+FAIETLTV LTNGKEKQLH+++IGCTES  GR+F GADGV+GLGT+ +SF Y+
Sbjct: 241 GASAKGLFAIETLTVGLTNGKEKQLHNSIIGCTESVQGRIFGGADGVIGLGTSSYSFTYK 300

Query: 301 ARQNANGGGFSYCLVDHLSNNSATSYFVLGPPA----VASRAATSLPSGNMTFTELFIGE 360
           A +NANGGGF+YCLVDHLS+ +ATSYF+LG P      A+ A++  P+GNM+FT+LF+G+
Sbjct: 301 AAENANGGGFAYCLVDHLSDRTATSYFILGNPISSTDSAAAASSVAPTGNMSFTKLFLGD 360

Query: 361 FFSSYYGVGLIGISADGVMLNIPPRVWDITDGGGTIVDSGTSLTMLAAPAFDMVMQALTP 420
            +SS+YGV L+GISADGVMLNIPPRVWDI  GGGTIVDSGTSLTMLAAPAFDMVM+AL P
Sbjct: 361 PYSSFYGVDLVGISADGVMLNIPPRVWDINSGGGTIVDSGTSLTMLAAPAFDMVMEALVP 420

Query: 421 KLKRFPKLQMEPFTFCFNNSEYLYEMTPQLRFHFSDGAVFEPPVKSYIITVSEWTMCVAV 480
           KLK F  +++EPF FCFNNS Y +EM P+LRFHF DG VF+PP KSYI++V E+  C+  
Sbjct: 421 KLKHFENIEIEPFDFCFNNSRYTHEMAPKLRFHFGDGTVFQPPPKSYIVSVGEYISCIGF 480

Query: 481 VSLAFPATNIIGNILQQNHLWQFDFFKRRIGFAPSDC 508
           VS+ FPATNIIGNILQQNHLW+FDF    +GFAPS+C
Sbjct: 481 VSMPFPATNIIGNILQQNHLWKFDFHAGTVGFAPSEC 512

[Close]

BLAST of Lcy07g014030 vs. NCBI nr
Match: XP_004140022.2 (aspartic proteinase NANA, chloroplast [Cucumis sativus] >KGN46781.1 hypothetical protein Csa_021058 [Cucumis sativus])

HSP 1 Score: 680.6 bits (1755), Expect = 1.0e-191
Identity = 337/539 (62.52%), Postives = 419/539 (77.74%), Query Frame = 0


Query: 1   MLDYRKPLIMSPVSNFS--ILFFSIFFFLSVHIAF----GD------------GNDDQEE 60
           ML YRKP  MSP+SNF     FF +FFFLS   +F    GD             ++D++E
Sbjct: 1   MLGYRKP--MSPISNFCFFFFFFLLFFFLSFSSSFLFALGDEDNNFNNNNNINDDEDEQE 60

Query: 61  AVRLELLHRHHPQVSEKLHGEAKLQGANERVKDIHDHDHNRHRFISESLNRTQTE-KKLM 120
            ++ +LLHRHHPQV+EK+HG+ K+Q  +ER+KDIH+HDHNRHR IS+S+N+ Q E  +L 
Sbjct: 61  IIKFDLLHRHHPQVAEKIHGDMKIQDVSERMKDIHEHDHNRHRSISKSMNQKQVEDARLR 120

Query: 121 AEGQMTTE---------PSSSATPIGMKMISGADYGASEYFVELKVGTPPQKCMLIADTG 180
           AE +  TE         P +++TPIGM+MISGAD+G+SEYFVELKVGTP Q  MLIADTG
Sbjct: 121 AEAEAATEEEVAKSAILPPATSTPIGMRMISGADFGSSEYFVELKVGTPAQTFMLIADTG 180

Query: 181 SDLTWVKCRYRRCMGNCSANPAHMIRNEKKERFPRAFHANRSSSFQPISCGK-KCMDDYA 240
           SDLTW+KCRYRRC GNCS+N  H  +NEKK+RF  AF AN SSSF+ +SC    C +D A
Sbjct: 181 SDLTWMKCRYRRCFGNCSSNVNHKSKNEKKQRFRHAFLANHSSSFKTVSCSSTMCTNDLA 240

Query: 241 TLFALPDCKTPTSPCRYDYGYSGGSSARGIFAIETLTVALTNGKEKQLHDTLIGCTESAT 300
            LFA+ +C  PTSPC YDY Y+GG+SA+GIFA ETLTV LTNGKEKQLH+++IGCTES  
Sbjct: 241 DLFAVRECHNPTSPCVYDYSYTGGASAKGIFAWETLTVGLTNGKEKQLHNSIIGCTESVQ 300

Query: 301 GRVFNGADGVLGLGTTVFSFPYRARQNANGGGFSYCLVDHLSNNSATSYFVLGPPAVASR 360
           G VF GADGV+GLGT+ +S  Y+A +NANGGGFSYCLVDHL++  A SYFVLG P  ++ 
Sbjct: 301 GSVFGGADGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAISYFVLGIPTPSTS 360

Query: 361 AATS---LPSGNMTFTELFIGEFFSSYYGVGLIGISADGVMLNIPPRVWDITDGGGTIVD 420
           A+TS   LP+  MT+T+L++G+ +SS+YGV LIGISA+G+MLNIP RVWDI  GGGTI+D
Sbjct: 361 ASTSSAKLPA-KMTYTKLYVGDPYSSFYGVDLIGISANGIMLNIPSRVWDINSGGGTIID 420

Query: 421 SGTSLTMLAAPAFDMVMQALTPKLKRFPKLQMEPFTFCFNNSEYLYEMTPQLRFHFSDGA 480
           SGTSLT+LAAPAFDMVM+ALTP+LK+F +L++EPF FCFNNS+Y +EM P+LRFHF DG 
Sbjct: 421 SGTSLTILAAPAFDMVMEALTPRLKKFQQLEIEPFDFCFNNSQYTHEMAPKLRFHFGDGT 480

Query: 481 VFEPPVKSYIITVSEWTMCVAVVSLAFPATNIIGNILQQNHLWQFDFFKRRIGFAPSDC 508
           VFEPP KSYI++V ++  C+  VS+ FPA NIIGNILQQNHLWQFDF KRR+GFAPS+C
Sbjct: 481 VFEPPTKSYIVSVGKFISCIGFVSMPFPANNIIGNILQQNHLWQFDFQKRRVGFAPSEC 536

[Close]

BLAST of Lcy07g014030 vs. NCBI nr
Match: XP_008456273.1 (PREDICTED: aspartic proteinase CDR1 [Cucumis melo])

HSP 1 Score: 653.7 bits (1685), Expect = 1.3e-183
Identity = 325/532 (61.09%), Postives = 409/532 (76.88%), Query Frame = 0


Query: 1   MLDYRKPLIMSPVSNFSILFFSIFFFLSVHIAF----GD------GNDDQEE--AVRLEL 60
           ML YRKP  MSP+SNF   FF + FFLS   +F    GD       NDD++E   +R +L
Sbjct: 1   MLGYRKP--MSPISNF-CFFFLLLFFLSFSSSFLFALGDEANNYNNNDDEDEQQTIRFDL 60

Query: 61  LHRHHPQVSEKLHGEAKLQGANERVKDIHDHDHNRHRFISESLNRTQTE-KKLMAEGQMT 120
           LHRHHPQVSEKL+G+ K+Q  +ER+KDIH+HD NRHR IS+S+N+ Q E  +L AE +  
Sbjct: 61  LHRHHPQVSEKLNGDMKIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAA 120

Query: 121 TE---------PSSSATPIGMKMISGADYGASEYFVELKVGTPPQKCMLIADTGSDLTWV 180
           T+         P +++TPIGMKMISGAD+G+SEYFV+LKVGTP Q  MLIADTGSDLTW+
Sbjct: 121 TQVEVAKSAILPPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWL 180

Query: 181 KCRYRRCMGNCSANPAHMIRNEKKERFPRAFHANRSSSFQPISCGK-KCMDDYATLFALP 240
           KCRYRRC GNCS N  H  +NEKK+RF  A  AN+SS+F+ +SC    C ++ A LFA+ 
Sbjct: 181 KCRYRRCFGNCSGNVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVA 240

Query: 241 DCKTPTSPCRYDYGYSGGSSARGIFAIETLTVALTNGKEKQLHDTLIGCTESATGRVFNG 300
           +C TPTSPC YDY Y+GG+SA+GIFA ETLTV LTNGKEKQL +++IGCTE   G VF+G
Sbjct: 241 ECDTPTSPCVYDYSYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDG 300

Query: 301 ADGVLGLGTTVFSFPYRARQNANGGGFSYCLVDHLSNNSATSYFVLGPPAVASRAATS-- 360
           ADGV+GLGT+ +S  Y+A +NANGGGFSYCLVDHL++  A SYFVLG P  ++ A+TS  
Sbjct: 301 ADGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSA 360

Query: 361 LPSGNMTFTELFIGEFFSSYYGVGLIGISADGVMLNIPPRVWDITDGGGTIVDSGTSLTM 420
            P   M++T+L++G+ +SS+YGV LIGISADG MLNIPPRVWD   G GTI+DSGTSLT+
Sbjct: 361 KPPAKMSYTKLYVGDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTV 420

Query: 421 LAAPAFDMVMQALTPKLKRFPKLQMEPFTFCFNNSEYLYEMTPQLRFHFSDGAVFEPPVK 480
           LA PAFD+VM+ LT +LK+F ++++EPF FCFNNS+Y ++M P+LRFHF DG VFEPP K
Sbjct: 421 LATPAFDVVMEVLTSRLKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTK 480

Query: 481 SYIITVSEWTMCVAVVSLAFPATNIIGNILQQNHLWQFDFFKRRIGFAPSDC 508
           SYI++V E+  C+ +VS+ FP+ NIIGNILQQNHLWQFDF KRR+GFA S+C
Sbjct: 481 SYIVSVGEFISCIGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASEC 529

[Close]

BLAST of Lcy07g014030 vs. NCBI nr
Match: KAA0033565.1 (aspartic proteinase CDR1 [Cucumis melo var. makuwa] >TYJ95622.1 aspartic proteinase CDR1 [Cucumis melo var. makuwa])

HSP 1 Score: 594.7 bits (1532), Expect = 7.3e-166
Identity = 285/456 (62.50%), Postives = 360/456 (78.95%), Query Frame = 0


Query: 65  KLQGANERVKDIHDHDHNRHRFISESLNRTQTE-KKLMAEGQMTTE---------PSSSA 124
           K+Q  +ER+KDIH+HD NRHR IS+S+N+ Q E  +L AE +  T+         P +++
Sbjct: 2   KIQDLHERMKDIHEHDRNRHRSISKSMNQKQIEDARLRAEAEAATQVEVAKSAILPPATS 61

Query: 125 TPIGMKMISGADYGASEYFVELKVGTPPQKCMLIADTGSDLTWVKCRYRRCMGNCSANPA 184
           TPIGMKMISGAD+G+SEYFV+LKVGTP Q  MLIADTGSDLTW+KCRYRRC GNCS N  
Sbjct: 62  TPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCRYRRCFGNCSGNVN 121

Query: 185 HMIRNEKKERFPRAFHANRSSSFQPISCGK-KCMDDYATLFALPDCKTPTSPCRYDYGYS 244
           H  +NEKK+RF  A  AN+SS+F+ +SC    C ++ A LFA+ +C TPTSPC YDY Y+
Sbjct: 122 HKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECDTPTSPCVYDYSYA 181

Query: 245 GGSSARGIFAIETLTVALTNGKEKQLHDTLIGCTESATGRVFNGADGVLGLGTTVFSFPY 304
           GG+SA+GIFA ETLTV LTNGKEKQL +++IGCTE   G VF+GADGV+GLGT+ +S  Y
Sbjct: 182 GGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQGNVFDGADGVMGLGTSSYSLTY 241

Query: 305 RARQNANGGGFSYCLVDHLSNNSATSYFVLGPPAVASRAATS--LPSGNMTFTELFIGEF 364
           +A +NANGGGFSYCLVDHL++  A SYFVLG P  ++ A+TS   P   M++T+L++G+ 
Sbjct: 242 KAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPPAKMSYTKLYVGDP 301

Query: 365 FSSYYGVGLIGISADGVMLNIPPRVWDITDGGGTIVDSGTSLTMLAAPAFDMVMQALTPK 424
           +SS+YGV LIGISADG MLNIPPRVWD   G GTI+DSGTSLT+LA PAFD+VM+ LT +
Sbjct: 302 YSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLATPAFDVVMEVLTSR 361

Query: 425 LKRFPKLQMEPFTFCFNNSEYLYEMTPQLRFHFSDGAVFEPPVKSYIITVSEWTMCVAVV 484
           LK+F ++++EPF FCFNNS+Y ++M P+LRFHF DG VFEPP KSYI++V E+  C+ +V
Sbjct: 362 LKQFQQIEIEPFNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSYIVSVGEFISCIGIV 421

Query: 485 SLAFPATNIIGNILQQNHLWQFDFFKRRIGFAPSDC 508
           S+ FP+ NIIGNILQQNHLWQFDF KRR+GFA S+C
Sbjct: 422 SMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASEC 457

[Close]

BLAST of Lcy07g014030 vs. NCBI nr
Match: XP_022943788.1 (aspartic proteinase NANA, chloroplast-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 594.0 bits (1530), Expect = 1.3e-165
Identity = 296/516 (57.36%), Postives = 373/516 (72.29%), Query Frame = 0


Query: 1   MLDYRKPLIMSPVSNFSILFFSIFFFLSVHIAFGDGNDDQEE-------AVRLELLHRHH 60
           ML Y  P  MSP+S   ++FF   FFLSVH+AF DG++ Q++        V+L+++HRHH
Sbjct: 1   MLGYTNP--MSPISPL-LIFFFFNFFLSVHVAF-DGDEQQQQQKPSEMPMVKLDVMHRHH 60

Query: 61  PQVSEKLHGEAKLQGANERVKDIHDHDHNRHRFISESLNRTQTEKKLMAEGQMTTEPSSS 120
           P V EKL+GE +  G+ +R +DIH+HDHNR R IS S+  ++T+++L         P  S
Sbjct: 61  PHVQEKLYGERRSLGSTDRFRDIHEHDHNRQRSISTSMKMSKTDRQL---------PMPS 120

Query: 121 ATPIGMKMISGADYGASEYFVELKVGTPPQKCMLIADTGSDLTWVKCRYRRCMGNCSANP 180
           + PI +K+ SG D+G +EYFV+ +VGTPPQK +LI DTGSDLTW+KCRYRRC+GNC+A+ 
Sbjct: 121 SAPIQLKISSGFDFGTNEYFVQFRVGTPPQKFLLIVDTGSDLTWLKCRYRRCLGNCTAHA 180

Query: 181 AHMIRNEKKERFPRAFHANRSSSFQPISCGKK-CMDDYATLFALPDCKTPTSPCRYDYGY 240
            H  R E K +F   F AN SSSF+ I+CG   C+ D   LFA+PDC+ P++PC YDY Y
Sbjct: 181 HHKSRVEHKVKFDHPFLANHSSSFKLITCGSDFCLGDLQLLFAIPDCQVPSNPCVYDYSY 240

Query: 241 SGGSSARGIFAIETLTVALTNGKEKQLHDTLIGCTESATGRVFNGADGVLGLGTTVFSFP 300
            GG +A G+FA ET+TV LTNGKEKQLHDTLIGCTE        G DG+LGLGT   SF 
Sbjct: 241 IGGGAATGLFANETVTVGLTNGKEKQLHDTLIGCTELFNAMQLKGVDGILGLGTGAHSFA 300

Query: 301 YRARQNANGGGFSYCLVDHLSNNSATSYFVLG-PPAVASRAATSLPSGNMTFTELFIGEF 360
           +RA  + NGGGFSYCL+DHLS++SATSYF+LG PPA     A   P GNMTF  L +G  
Sbjct: 301 HRAALDKNGGGFSYCLIDHLSHHSATSYFILGYPPAEPLSVA---PVGNMTFINLHLGGP 360

Query: 361 FSSYYGVGLIGISADGVMLNIPPRVWDITDGGGTIVDSGTSLTMLAAPAFDMVMQALTPK 420
           F+SYYGVGLIGIS DGV LNIPPRVWDI  GGGTI+DSGTSL+ML APAFD+ M+A+  K
Sbjct: 361 FNSYYGVGLIGISIDGVTLNIPPRVWDIQKGGGTILDSGTSLSMLTAPAFDVFMEAMVQK 420

Query: 421 LKRFPKLQMEPFTFCFNNSEYLYEMTPQLRFHFSDGAVFEPPVKSYIITVSEWTMCVAVV 480
           LK+F ++  +PF +CFN + Y +EM P+LRFHF  G VFEPP KSYI+ V +  +C+   
Sbjct: 421 LKKFQQILADPFAYCFNKTHYSHEMAPKLRFHFEKGVVFEPPPKSYIVKVDD-ILCLGFT 480

Query: 481 SLAFPATNIIGNILQQNHLWQFDFFKRRIGFAPSDC 508
           S+ FP TNIIGNILQQN LWQFDFF +++GFAPS C
Sbjct: 481 SIPFPDTNIIGNILQQNFLWQFDFFNKKVGFAPSQC 499

[Close]

BLAST of Lcy07g014030 vs. TAIR 10
Match: AT3G12700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 321.2 bits (822), Expect = 1.5e-87
Identity = 192/474 (40.51%), Postives = 258/474 (54.43%), Query Frame = 0


Query: 40  QEEAVRLELLHRHHPQVSEKLHGEAKLQGANERVKDIHDHDHNRHRFISESLNRTQTEKK 99
           ++ +VRL+L HR           +  L     R++D+   D  RH  IS   N T     
Sbjct: 45  KDTSVRLKLAHR-----------DTLLPKPLSRIEDVIGADQKRHSLISRKRNST----- 104

Query: 100 LMAEGQMTTEPSSSATPIGMKMI--SGADYGASEYFVELKVGTPPQKCMLIADTGSDLTW 159
                            +G+KM   SG DYG ++YF E++VGTP +K  ++ DTGS+LTW
Sbjct: 105 -----------------VGVKMDLGSGIDYGTAQYFTEIRVGTPAKKFRVVVDTGSELTW 164

Query: 160 VKCRYRRCMGNCSANPAHMIRNEKKERFPRAFHANRSSSFQPISC-GKKCMDDYATLFAL 219
           V CRYR                 K  R  R F A+ S SF+ + C  + C  D   LF+L
Sbjct: 165 VNCRYR--------------ARGKDNR--RVFRADESKSFKTVGCLTQTCKVDLMNLFSL 224

Query: 220 PDCKTPTSPCRYDYGYSGGSSARGIFAIETLTVALTNGKEKQLHDTLIGCTESATGRVFN 279
             C TP++PC YDY Y+ GS+A+G+FA ET+TV LTNG+  +L   LIGC+ S TG+ F 
Sbjct: 225 TTCPTPSTPCSYDYRYADGSAAQGVFAKETITVGLTNGRMARLPGHLIGCSSSFTGQSFQ 284

Query: 280 GADGVLGLGTTVFSFPYRARQNANGGGFSYCLVDHLSNNSATSYFVLGPPAVASRAATSL 339
           GADGVLGL  + FSF   A  +  G  FSYCLVDHLSN + ++Y + G     S  +T  
Sbjct: 285 GADGVLGLAFSDFSFTSTA-TSLYGAKFSYCLVDHLSNKNVSNYLIFG-----SSRSTKT 344

Query: 340 PSGNMTFTELFIGEFFSSYYGVGLIGISADGVMLNIPPRVWDITDGGGTIVDSGTSLTML 399
                T  +L        +Y + +IGIS    ML+IP +VWD T GGGTI+DSGTSLT+L
Sbjct: 345 AFRRTTPLDL---TRIPPFYAINVIGISLGYDMLDIPSQVWDATSGGGTILDSGTSLTLL 404

Query: 400 AAPAFDMVMQALTPKLKRFPKLQME--PFTFCFN-NSEYLYEMTPQLRFHFSDGAVFEPP 459
           A  A+  V+  L   L    +++ E  P  +CF+  S +     PQL FH   GA FEP 
Sbjct: 405 ADAAYKQVVTGLARYLVELKRVKPEGVPIEYCFSFTSGFNVSKLPQLTFHLKGGARFEPH 460

Query: 460 VKSYIITVSEWTMCVAVVSLAFPATNIIGNILQQNHLWQFDFFKRRIGFAPSDC 508
            KSY++  +    C+  VS   PATN+IGNI+QQN+LW+FD     + FAPS C
Sbjct: 465 RKSYLVDAAPGVKCLGFVSAGTPATNVIGNIMQQNYLWEFDLMASTLSFAPSAC 460

[Close]

BLAST of Lcy07g014030 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 231.1 bits (588), Expect = 2.0e-60
Identity = 151/407 (37.10%), Postives = 215/407 (52.83%), Query Frame = 0


Query: 121 MISGADYGASEYFVELKVGTPPQKCMLIADTGSDLTWVKCRYRRCMGNCS-ANPAHMIRN 180
           ++SGA  G+ +YFV+L++G PPQ  +LIADTGSDL WVKC   R   NCS  +PA +   
Sbjct: 73  VVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCSACR---NCSHHSPATVF-- 132

Query: 181 EKKERFPRAFHANRSSSFQPISCGKKCMDDYATLFALPDCKTPT-------SPCRYDYGY 240
                FPR      SS+F P      C D    L   PD + P        S C Y+YGY
Sbjct: 133 -----FPR-----HSSTFSP----AHCYDPVCRLVPKPD-RAPICNHTRIHSTCHYEYGY 192

Query: 241 SGGSSARGIFAIETLTVALTNGKEKQLHDTLIGC-----TESATGRVFNGADGVLGLGTT 300
           + GS   G+FA ET ++  ++GKE +L     GC      +S +G  FNGA+GV+GLG  
Sbjct: 193 ADGSLTSGLFARETTSLKTSSGKEARLKSVAFGCGFRISGQSVSGTSFNGANGVMGLGRG 252

Query: 301 VFSFPYRARQNANGGGFSYCLVDHLSNNSATSYFVLGPPAVASRAATSLPSGNMTFTELF 360
             SF  +  +   G  FSYCL+D+  +   TSY ++G                + FT L 
Sbjct: 253 PISFASQLGRRF-GNKFSYCLMDYTLSPPPTSYLIIGNGGDG--------ISKLFFTPLL 312

Query: 361 IGEFFSSYYGVGLIGISADGVMLNIPPRVWDITD--GGGTIVDSGTSLTMLAAPAFDMVM 420
                 ++Y V L  +  +G  L I P +W+I D   GGT+VDSGT+L  LA PA+  V+
Sbjct: 313 TNPLSPTFYYVKLKSVFVNGAKLRIDPSIWEIDDSGNGGTVVDSGTTLAFLAEPAYRSVI 372

Query: 421 QALTPKLKRFPKLQMEP-FTFCFNNSEYL--YEMTPQLRFHFSDGAVFEPPVKSYIITVS 480
            A+  ++K      + P F  C N S      ++ P+L+F FS GAVF PP ++Y I   
Sbjct: 373 AAVRRRVKLPIADALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYFIETE 432

Query: 481 EWTMCVAVVSL-AFPATNIIGNILQQNHLWQFDFFKRRIGFAPSDCA 509
           E   C+A+ S+      ++IGN++QQ  L++FD  + R+GF+   CA
Sbjct: 433 EQIQCLAIQSVDPKVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCA 450

[Close]

BLAST of Lcy07g014030 vs. TAIR 10
Match: AT3G59080.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 199.1 bits (505), Expect = 8.4e-51
Identity = 144/451 (31.93%), Postives = 225/451 (49.89%), Query Frame = 0


Query: 72  RVKDIHDHDHNRHRFISESLNRTQTEKKLMAEGQMTTEPSSSATPIGMKMISGADYGASE 131
           R++ +H     ++   + S  + + +K+++    + +     A  +   + SG   G+ E
Sbjct: 110 RIQTLHKRVLEKNNQNTVSQKQKKNDKEVVTTTPVASSVEEQAGQLVATLESGMTLGSGE 169

Query: 132 YFVELKVGTPPQKCMLIADTGSDLTWVKCRYRRCMGNCSANPAHMIRNEKKERFPRAFHA 191
           YF+++ VG+PP+   LI DTGSDL W++C    C      N A              +  
Sbjct: 170 YFMDVLVGSPPKHFSLILDTGSDLNWIQC--LPCYDCFQQNGAF-------------YDP 229

Query: 192 NRSSSFQPISCGKKCMDDYATLFALPD----CKTPTSPCRYDYGYSGGSSARGIFAIETL 251
             S+S++ I+C     D    L + PD    CK+    C Y Y Y   S+  G FA+ET 
Sbjct: 230 KASASYKNITCN----DQRCNLVSSPDPPMPCKSDNQSCPYYYWYGDSSNTTGDFAVETF 289

Query: 252 TVAL-TNGKEKQLHDT---LIGCTESATGRVFNGADGVLGLGTTVFSFPYRARQNANGGG 311
           TV L TNG   +L++    + GC     G +F+GA G+LGLG    SF  +  Q+  G  
Sbjct: 290 TVNLTTNGGSSELYNVENMMFGCGHWNRG-LFHGAAGLLGLGRGPLSFSSQL-QSLYGHS 349

Query: 312 FSYCLVDHLSNNSATSYFVLGPPAVASRAATSLPSGNMTFTELFIGE--FFSSYYGVGLI 371
           FSYCLVD  S+ + +S  + G           L   N+ FT    G+     ++Y V + 
Sbjct: 350 FSYCLVDRNSDTNVSSKLIFG------EDKDLLSHPNLNFTSFVAGKENLVDTFYYVQIK 409

Query: 372 GISADGVMLNIPPRVWDI-TDG-GGTIVDSGTSLTMLAAPAFDMVMQALTPKLK-RFPKL 431
            I   G +LNIP   W+I +DG GGTI+DSGT+L+  A PA++ +   +  K K ++P  
Sbjct: 410 SILVAGEVLNIPEETWNISSDGAGGTIIDSGTTLSYFAEPAYEFIKNKIAEKAKGKYPVY 469

Query: 432 QMEPFTF-CFNNSEYLYEMTPQLRFHFSDGAVFEPPVKSYIITVSEWTMCVAVVSLAFPA 491
           +  P    CFN S       P+L   F+DGAV+  P ++  I ++E  +C+A++     A
Sbjct: 470 RDFPILDPCFNVSGIHNVQLPELGIAFADGAVWNFPTENSFIWLNEDLVCLAMLGTPKSA 529

Query: 492 TNIIGNILQQNHLWQFDFFKRRIGFAPSDCA 509
            +IIGN  QQN    +D  + R+G+AP+ CA
Sbjct: 530 FSIIGNYQQQNFHILYDTKRSRLGYAPTKCA 533

[Close]

BLAST of Lcy07g014030 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 187.6 bits (475), Expect = 2.5e-47
Identity = 144/453 (31.79%), Postives = 220/453 (48.57%), Query Frame = 0


Query: 72  RVKDIHDHDHNRHRFISESLNRTQTEKKLMAEGQMTTEPSSSATPIGMKMISGADYGASE 131
           R+K +H   +      S+     +  KK+ ++  +   P  S   +   + SG   G+ E
Sbjct: 105 RIKTLHARFNK-----SKKQKNEKVRKKITSDISLVGAPEVSPGKLIATLESGMTLGSGE 164

Query: 132 YFVELKVGTPPQKCMLIADTGSDLTWVKCRYRRCMGNCSANPAHMIRNEKKERFPRAFHA 191
           YF+++ VGTPP+   LI DTGSDL W++C            P +   ++        +  
Sbjct: 165 YFMDVLVGTPPKHFSLILDTGSDLNWLQCL-----------PCYDCFHQN----GMFYDP 224

Query: 192 NRSSSFQPISCGKKCMDDYATLFALPD----CKTPTSPCRYDYGYSGGSSARGIFAIETL 251
             S+SF+ I+C     D   +L + PD    C++    C Y Y Y   S+  G FA+ET 
Sbjct: 225 KTSASFKNITCN----DPRCSLISSPDPPVQCESDNQSCPYFYWYGDRSNTTGDFAVETF 284

Query: 252 TVALT----NGKEKQLHDTLIGCTESATGRVFNGADGVLGLGTTVFSFPYRARQNANGGG 311
           TV LT       E ++ + + GC     G +F+GA G+LGLG    SF  +  Q+  G  
Sbjct: 285 TVNLTTTEGGSSEYKVGNMMFGCGHWNRG-LFSGASGLLGLGRGPLSFSSQL-QSLYGHS 344

Query: 312 FSYCLVDHLSNNSATSYFVLGPPAVASRAATSLPSGNMTFTELFIGEFFS--SYYGVGLI 371
           FSYCLVD  SN + +S  + G           L   N+ FT    G+  S  ++Y + + 
Sbjct: 345 FSYCLVDRNSNTNVSSKLIFG------EDKDLLNHTNLNFTSFVNGKENSVETFYYIQIK 404

Query: 372 GISADGVMLNIPPRVWDI-TDG-GGTIVDSGTSLTMLAAPAFDMVMQALTPKLK-RFPKL 431
            I   G  L+IP   W+I +DG GGTI+DSGT+L+  A PA++++      K+K  +P  
Sbjct: 405 SILVGGKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAEKMKENYPIF 464

Query: 432 QMEP-FTFCFNNS--EYLYEMTPQLRFHFSDGAVFEPPVKSYIITVSEWTMCVAVVSLAF 491
           +  P    CFN S  E      P+L   F DG V+  P ++  I +SE  +C+A++    
Sbjct: 465 RDFPVLDPCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLSEDLVCLAILGTPK 524

Query: 492 PATNIIGNILQQNHLWQFDFFKRRIGFAPSDCA 509
              +IIGN  QQN    +D  + R+GF P+ CA
Sbjct: 525 STFSIIGNYQQQNFHILYDTKRSRLGFTPTKCA 525

[Close]

BLAST of Lcy07g014030 vs. TAIR 10
Match: AT3G59080.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 177.2 bits (448), Expect = 3.4e-44
Identity = 133/447 (29.75%), Postives = 209/447 (46.76%), Query Frame = 0


Query: 72  RVKDIHDHDHNRHRFISESLNRTQTEKKLMAEGQMTTEPSSSATPIGMKMISGADYGASE 131
           R++ +H     ++   + S  + + +K+++    + +     A  +   + SG   G+ E
Sbjct: 110 RIQTLHKRVLEKNNQNTVSQKQKKNDKEVVTTTPVASSVEEQAGQLVATLESGMTLGSGE 169

Query: 132 YFVELKVGTPPQKCMLIADTGSDLTWVKCRYRRCMGNCSANPAHMIRNEKKERFPRAFHA 191
           YF+++ VG+PP+   LI DTGSDL W++C                               
Sbjct: 170 YFMDVLVGSPPKHFSLILDTGSDLNWIQC------------------------------- 229

Query: 192 NRSSSFQPISCGKKCMDDYATLFALPDCKTPTSPCRYDYGYSGGSSARGIFAIETLTVAL 251
                         C D +         +     C Y Y Y   S+  G FA+ET TV L
Sbjct: 230 ------------LPCYDCFQ--------QNDNQSCPYYYWYGDSSNTTGDFAVETFTVNL 289

Query: 252 -TNGKEKQLHDT---LIGCTESATGRVFNGADGVLGLGTTVFSFPYRARQNANGGGFSYC 311
            TNG   +L++    + GC     G +F+GA G+LGLG    SF  +  Q+  G  FSYC
Sbjct: 290 TTNGGSSELYNVENMMFGCGHWNRG-LFHGAAGLLGLGRGPLSFSSQL-QSLYGHSFSYC 349

Query: 312 LVDHLSNNSATSYFVLGPPAVASRAATSLPSGNMTFTELFIGE--FFSSYYGVGLIGISA 371
           LVD  S+ + +S  + G           L   N+ FT    G+     ++Y V +  I  
Sbjct: 350 LVDRNSDTNVSSKLIFG------EDKDLLSHPNLNFTSFVAGKENLVDTFYYVQIKSILV 409

Query: 372 DGVMLNIPPRVWDI-TDG-GGTIVDSGTSLTMLAAPAFDMVMQALTPKLK-RFPKLQMEP 431
            G +LNIP   W+I +DG GGTI+DSGT+L+  A PA++ +   +  K K ++P  +  P
Sbjct: 410 AGEVLNIPEETWNISSDGAGGTIIDSGTTLSYFAEPAYEFIKNKIAEKAKGKYPVYRDFP 469

Query: 432 FTF-CFNNSEYLYEMTPQLRFHFSDGAVFEPPVKSYIITVSEWTMCVAVVSLAFPATNII 491
               CFN S       P+L   F+DGAV+  P ++  I ++E  +C+A++     A +II
Sbjct: 470 ILDPCFNVSGIHNVQLPELGIAFADGAVWNFPTENSFIWLNEDLVCLAMLGTPKSAFSII 497

Query: 492 GNILQQNHLWQFDFFKRRIGFAPSDCA 509
           GN  QQN    +D  + R+G+AP+ CA
Sbjct: 530 GNYQQQNFHILYDTKRSRLGYAPTKCA 497

The following BLAST results are available for this feature:

BLAST of Lcy07g014030 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LTW4	2.1e-86	40.51	Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE...	[more]
Q9LHE3	3.0e-37	27.59	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LNJ3	1.9e-36	32.83	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q9LS40	2.8e-35	28.21	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q6XBF8	3.9e-29	27.08	Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1	[more]

BLAST of Lcy07g014030 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KG92	4.9e-192	62.52	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G13439...	[more]
A0A1S3C2F3	6.4e-184	61.09	aspartic proteinase CDR1 OS=Cucumis melo OX=3656 GN=LOC103496268 PE=3 SV=1	[more]
A0A5D3B701	3.6e-166	62.50	Aspartic proteinase CDR1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A6J1FXD5	6.1e-166	57.36	aspartic proteinase NANA, chloroplast-like isoform X1 OS=Cucurbita moschata OX=3...	[more]
A0A6J1FVB3	8.8e-165	57.59	aspartic proteinase NANA, chloroplast-like isoform X2 OS=Cucurbita moschata OX=3...	[more]

BLAST of Lcy07g014030 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038901983.1	1.3e-199	66.34	aspartic proteinase NANA, chloroplast [Benincasa hispida]	[more]
XP_004140022.2	1.0e-191	62.52	aspartic proteinase NANA, chloroplast [Cucumis sativus] >KGN46781.1 hypothetical...	[more]
XP_008456273.1	1.3e-183	61.09	PREDICTED: aspartic proteinase CDR1 [Cucumis melo]	[more]
KAA0033565.1	7.3e-166	62.50	aspartic proteinase CDR1 [Cucumis melo var. makuwa] >TYJ95622.1 aspartic protein...	[more]
XP_022943788.1	1.3e-165	57.36	aspartic proteinase NANA, chloroplast-like isoform X1 [Cucurbita moschata]	[more]

BLAST of Lcy07g014030 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12700.1	1.5e-87	40.51	Eukaryotic aspartyl protease family protein	[more]
AT3G25700.1	2.0e-60	37.10	Eukaryotic aspartyl protease family protein	[more]
AT3G59080.1	8.4e-51	31.93	Eukaryotic aspartyl protease family protein	[more]
AT2G42980.1	2.5e-47	31.79	Eukaryotic aspartyl protease family protein	[more]
AT3G59080.2	3.4e-44	29.75	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 138..158 score: 49.74 coord: 385..396 score: 48.62 coord: 479..494 score: 23.01
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 132..324 e-value: 2.0E-43 score: 148.6
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 112..324 e-value: 3.6E-38 score: 133.6
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 335..509 e-value: 1.3E-37 score: 131.3
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 124..507
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 356..503 e-value: 1.2E-27 score: 96.7
None	No IPR available	PANTHER	PTHR47967:SF69	ASPARTIC PROTEINASE NANA, CHLOROPLAST	coord: 29..507
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 29..507
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 147..158
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 132..503 score: 36.0312
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 131..507 e-value: 1.01542E-79 score: 247.947

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy07g014030.1	Lcy07g014030.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004190	aspartic-type endopeptidase activity