Lcy07g013630 (gene) Sponge gourd (P93075) v1

Overview
NameLcy07g013630
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRING-type domain-containing protein
LocationChr07: 42761317 .. 42763127 (+)
RNA-Seq ExpressionLcy07g013630
SyntenyLcy07g013630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCAGTTCTCTCTCTCAAGGGATTTGTTTTTGTTTTTCTTGAAGCCTCTGCCCAGATTAAACCTAAATTTCAAGTTCCGAGAAGAACGATTCAATTCGTCTTGGAAAGAAGAAGAAGAAGAACAGCAGAAGGAGAGATTGATTGATTTCGTTTGGTTGAATCGGTTATATTATTATGTGAAATTGGTTTTTTCTGTAAGAGATCATGGGATACGGAGCGCCATTAACAGGTTTCAGTGCTAATGCTGCAGCGGCGGAGACGACTCTGCTTCCGCTTCCGCTTCCGCCGTACAATTCTGTAGCGATCGATTCGTTTCCCAAGGCGGCGGTTGCGGCGACGGCGACTGCGTCGACGGCCGCTGTTATGAAGTCCGATAGCGGTATTACATATAATCTTCCGATTCCGAGGAAGCGCGCTAGGGATCATTCCACCATTAACCGATTCGTCTCGTATCCGTCGCATGCTCATTCTCAGAAGAATTGTGGATGTAATTACTATTTTCTCGGCGAAGATATCTCCATCCAAATTCAACAGCAGCAATTCGATCTCGATCGATTGATATCGCAACATGTACGGTACTAAAACGCTCTTTAATTAATTTCTTTCAGTCGACGGAATTTTAATTTATTTATTCATATGCGAAAAGTGCTGGTTGAGAAACTTCTTGCGGTTGTTAATTTATATATTTCTGTGATAATTTTGGGGAGCGCAGATGGAGAAGGTGAGGATTGAAGTGGAGGAAAGACGGAAGAGACAAGCGAGGAGGATCTTGGAGGCGATAGAATTAGGAATGATGAGGATACTGAGGAGCAAAGAGGAAGAAATCGAGAAGATCGGGAAATTAAATTGGGAACTAGAAGAGCGAGTGAATTCTCTATGCATGGAGAATCAAATATGGCGCGATGTGGCTCAGACGAACGAAGCCACCGCCAATGCCCTCCGTAGCGACCTGGAACAAGTCCTGTTACAGGTGAAAACCGAGGATCCAAGCACAACCGCGGCCGCCGCACTGGCCGACGACGCAGAGTCGTGCTGCGGAAGCAACGACGAGGGACGGTGGCGGAATTCGGATGAAGTTGAAGAATGGGAATTAGAGAGGAAGAGAGGGAGGAAGACGATGAGAAAGGGAAAGGAAGACAGACGGTGCAAAAACTGTTGGAAAGAAGAATCGTGCGTGTTGCTATTGCCATGCAGGCATCTGTGTTTGTGTACTGTTTGTGGGTCATCTCTCCACACTTGTCCAATCTGCAACTCTACCAATAACGCCAGTGTTCGTGTAATCATGCCTTAGAAATTCACTTCACATTACATTACAATCCAAATTCTTCTCTTCTTTTAATTCGTATATCTGTTCTCTTCTTGCTGCGTGCGTGCTGTTTTAATTGTCAACATTTTACCGATGGTATTGAGCATAATTTAGTGAAAGATGAGAGTAAGTTAGTGGAGTAGTGTTGGAAGGATACGGGGGGGCCATTGAGTGTTTGGGACAAGTCATGGGTGTAATGAAAGAAAAAAAAATAATAAAAAAAGAAAGAAAGAAAGAAGGGAATAGGTGGAGGTTTAAAAGCTGACACGTTGGACTCCCGCAAATATCGCTGTGGGGGGGAGAGCAGTGATATCAGGTACGGCCCACCTGCAACACACAGCCTTTTTACCTTACACTTACACTTACTTTTCACACCCCACACGGATTCCGATTCCTAATGCTTCCAAAAAAATATCCTAATTCTTTCTTTTTTAGACTTACTAGTAATTATGGGAATCTAATTCTTTAATAATTAAAAAGAAAAAGAGAGAGAGAGAGAGAAATT

mRNA sequence

TTCAGTTCTCTCTCTCAAGGGATTTGTTTTTGTTTTTCTTGAAGCCTCTGCCCAGATTAAACCTAAATTTCAAGTTCCGAGAAGAACGATTCAATTCGTCTTGGAAAGAAGAAGAAGAAGAACAGCAGAAGGAGAGATTGATTGATTTCGTTTGGTTGAATCGGTTATATTATTATGTGAAATTGGTTTTTTCTGTAAGAGATCATGGGATACGGAGCGCCATTAACAGGTTTCAGTGCTAATGCTGCAGCGGCGGAGACGACTCTGCTTCCGCTTCCGCTTCCGCCGTACAATTCTGTAGCGATCGATTCGTTTCCCAAGGCGGCGGTTGCGGCGACGGCGACTGCGTCGACGGCCGCTGTTATGAAGTCCGATAGCGGTATTACATATAATCTTCCGATTCCGAGGAAGCGCGCTAGGGATCATTCCACCATTAACCGATTCGTCTCGTATCCGTCGCATGCTCATTCTCAGAAGAATTGTGGATGTAATTACTATTTTCTCGGCGAAGATATCTCCATCCAAATTCAACAGCAGCAATTCGATCTCGATCGATTGATATCGCAACATATGGAGAAGGTGAGGATTGAAGTGGAGGAAAGACGGAAGAGACAAGCGAGGAGGATCTTGGAGGCGATAGAATTAGGAATGATGAGGATACTGAGGAGCAAAGAGGAAGAAATCGAGAAGATCGGGAAATTAAATTGGGAACTAGAAGAGCGAGTGAATTCTCTATGCATGGAGAATCAAATATGGCGCGATGTGGCTCAGACGAACGAAGCCACCGCCAATGCCCTCCGTAGCGACCTGGAACAAGTCCTGTTACAGGTGAAAACCGAGGATCCAAGCACAACCGCGGCCGCCGCACTGGCCGACGACGCAGAGTCGTGCTGCGGAAGCAACGACGAGGGACGGTGGCGGAATTCGGATGAAGTTGAAGAATGGGAATTAGAGAGGAAGAGAGGGAGGAAGACGATGAGAAAGGGAAAGGAAGACAGACGGTGCAAAAACTGTTGGAAAGAAGAATCGTGCGTGTTGCTATTGCCATGCAGGCATCTGTGTTTGTGTACTGTTTGTGGGTCATCTCTCCACACTTGTCCAATCTGCAACTCTACCAATAACGCCAGTGTTCGTGTAATCATGCCTTAGAAATTCACTTCACATTACATTACAATCCAAATTCTTCTCTTCTTTTAATTCGTATATCTGTTCTCTTCTTGCTGCGTGCGTGCTGTTTTAATTGTCAACATTTTACCGATGGTATTGAGCATAATTTAGTGAAAGATGAGAGTAAGTTAGTGGAGTAGTGTTGGAAGGATACGGGGGGGCCATTGAGTGTTTGGGACAAGTCATGGGTGTAATGAAAGAAAAAAAAATAATAAAAAAAGAAAGAAAGAAAGAAGGGAATAGGTGGAGGTTTAAAAGCTGACACGTTGGACTCCCGCAAATATCGCTGTGGGGGGGAGAGCAGTGATATCAGGTACGGCCCACCTGCAACACACAGCCTTTTTACCTTACACTTACACTTACTTTTCACACCCCACACGGATTCCGATTCCTAATGCTTCCAAAAAAATATCCTAATTCTTTCTTTTTTAGACTTACTAGTAATTATGGGAATCTAATTCTTTAATAATTAAAAAGAAAAAGAGAGAGAGAGAGAGAAATT

Coding sequence (CDS)

ATGGGATACGGAGCGCCATTAACAGGTTTCAGTGCTAATGCTGCAGCGGCGGAGACGACTCTGCTTCCGCTTCCGCTTCCGCCGTACAATTCTGTAGCGATCGATTCGTTTCCCAAGGCGGCGGTTGCGGCGACGGCGACTGCGTCGACGGCCGCTGTTATGAAGTCCGATAGCGGTATTACATATAATCTTCCGATTCCGAGGAAGCGCGCTAGGGATCATTCCACCATTAACCGATTCGTCTCGTATCCGTCGCATGCTCATTCTCAGAAGAATTGTGGATGTAATTACTATTTTCTCGGCGAAGATATCTCCATCCAAATTCAACAGCAGCAATTCGATCTCGATCGATTGATATCGCAACATATGGAGAAGGTGAGGATTGAAGTGGAGGAAAGACGGAAGAGACAAGCGAGGAGGATCTTGGAGGCGATAGAATTAGGAATGATGAGGATACTGAGGAGCAAAGAGGAAGAAATCGAGAAGATCGGGAAATTAAATTGGGAACTAGAAGAGCGAGTGAATTCTCTATGCATGGAGAATCAAATATGGCGCGATGTGGCTCAGACGAACGAAGCCACCGCCAATGCCCTCCGTAGCGACCTGGAACAAGTCCTGTTACAGGTGAAAACCGAGGATCCAAGCACAACCGCGGCCGCCGCACTGGCCGACGACGCAGAGTCGTGCTGCGGAAGCAACGACGAGGGACGGTGGCGGAATTCGGATGAAGTTGAAGAATGGGAATTAGAGAGGAAGAGAGGGAGGAAGACGATGAGAAAGGGAAAGGAAGACAGACGGTGCAAAAACTGTTGGAAAGAAGAATCGTGCGTGTTGCTATTGCCATGCAGGCATCTGTGTTTGTGTACTGTTTGTGGGTCATCTCTCCACACTTGTCCAATCTGCAACTCTACCAATAACGCCAGTGTTCGTGTAATCATGCCTTAG

Protein sequence

MGYGAPLTGFSANAAAAETTLLPLPLPPYNSVAIDSFPKAAVAATATASTAAVMKSDSGITYNLPIPRKRARDHSTINRFVSYPSHAHSQKNCGCNYYFLGEDISIQIQQQQFDLDRLISQHMEKVRIEVEERRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCMENQIWRDVAQTNEATANALRSDLEQVLLQVKTEDPSTTAAAALADDAESCCGSNDEGRWRNSDEVEEWELERKRGRKTMRKGKEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP
Homology
BLAST of Lcy07g013630 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 7.0e-61
Identity = 142/292 (48.63%), Postives = 195/292 (66.78%), Query Frame = 0

Query: 27  PPYNSVAIDSFPKAAVAAT-ATASTAAVMKSDSGITYNLPI-PRKRARDHSTINRFVSYP 86
           P YN   +D     ++  T  +  ++    SD+ + +  P+  RKR+R+ S +   ++  
Sbjct: 54  PIYNISPVDRLVHQSMKPTIQSVDSSLTFNSDNNVDFLRPVSSRKRSREESVV---LNPS 113

Query: 87  SHAHSQKNCGCNYYFLGEDISIQIQQQQFDLDRLISQHMEKVRIEVEERRKRQARRILEA 146
           ++   QKN      FLG+D+S  +QQ  FD+DRLIS H+E++R+E+EE+RK Q RRI+EA
Sbjct: 114 AYMQIQKNPTDPLMFLGQDLSSNVQQHHFDIDRLISNHVERMRMEIEEKRKTQGRRIVEA 173

Query: 147 IELGMMRILRSKEEEIEKIGKLNWELEERVNSLCMENQIWRDVAQTNEATANALRSDLEQ 206
           +E G+M+ LR+K++EI  IGKLN  LEE+V SLC+ENQIWRDVAQ+NEAT NALRS+L+Q
Sbjct: 174 VEQGLMKTLRAKDDEINHIGKLNLFLEEKVKSLCVENQIWRDVAQSNEATVNALRSNLQQ 233

Query: 207 VLLQV---KTEDPSTTAAAALADDAESCCGSNDEGRWRNSDEVEEWEL--ERKRGRKTMR 266
           VL  V   + E+P T     +ADDA+SCCGSNDEG      E E W+L  E +  +K  R
Sbjct: 234 VLAAVERNRWEEPPT-----VADDAQSCCGSNDEG----DSEEERWKLAGEAQDTKKMCR 293

Query: 267 KGKEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHTCPICNSTNNASVRV 312
            G     C++C K E+ VLLLPCRH+CLC+VCGSSL+TCPIC S   AS+ V
Sbjct: 294 VGMS--MCRSCGKGEASVLLLPCRHMCLCSVCGSSLNTCPICKSPKTASLHV 331

BLAST of Lcy07g013630 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 4.1e-53
Identity = 146/326 (44.79%), Postives = 188/326 (57.67%), Query Frame = 0

Query: 9   GFSANAAAAETTLLPLPLPPYNSVAIDSFPKA--AVAATATASTAAVMKSDSGITYN--- 68
           GF  N       + P  +P + S    + P        + T  T A   +D     +   
Sbjct: 34  GFCNNNQTGYGVVSPFSVPNHTSTTTTATPPLLHVYGGSDTIPTTAGYYADGATNLDCEF 93

Query: 69  LPIP-RKRARDHSTIN--RFVSYPSHAHSQKNCGCN------YYFLGEDISI--QIQQQQ 128
            P+P RKR+RD S  N    +     + S  N          + FLG+DI I   + QQQ
Sbjct: 94  FPLPTRKRSRDSSRSNYHHLLLQNPRSSSCVNAATTTTTTTLFSFLGQDIDISSHMNQQQ 153

Query: 129 FDLDRLISQH---MEKVRIEVEERRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWE 188
            ++DR +S H   ME+V+ E+EE+RKRQAR I+EAIE G+++ LR KEEE E+IGK+N  
Sbjct: 154 HEIDRFVSLHLYQMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHA 213

Query: 189 LEERVNSLCMENQIWRDVAQTNEATANALRSDLEQVLLQVKTEDPSTTAAAAL--ADDAE 248
           LEERV SL +ENQIWRD+AQTNEATAN LR++LE VL QVK           +   DDAE
Sbjct: 214 LEERVKSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEKNMNEEDDAE 273

Query: 249 SCCGSNDEGRWRNSDEVEEWELERKRGRKTMRKGKEDRRCKNCWKEESCVLLLPCRHLCL 308
           SCCGS+  G     +E     +  +R  +   + +  R C+NC +EESCVLLLPCRHLCL
Sbjct: 274 SCCGSSCGG---GGEETVRRRVGLEREAQDKAERRRRRMCRNCGEEESCVLLLPCRHLCL 333

Query: 309 CTVCGSSLHTCPICNSTNNASVRVIM 314
           C VCGSS+HTCPIC S  NASV V M
Sbjct: 334 CGVCGSSVHTCPICTSPKNASVHVNM 356

BLAST of Lcy07g013630 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 6.2e-41
Identity = 120/281 (42.70%), Postives = 162/281 (57.65%), Query Frame = 0

Query: 43  AATATASTAAVMKSDSGITYNLPIP---RKRARD--HSTINRFVSYPSHAHSQKNCGCNY 102
           A    A+ A   K++SG++YN  +P    KR RD   S  N  V   S A    +     
Sbjct: 49  AVEPLAAKANFNKAESGLSYNFTVPPLSTKRQRDFQFSDSNAPVKRRSVAFDSSSPS--- 108

Query: 103 YFLGEDISIQIQ-QQQFDLDRLISQHMEKVRIEVEERRKRQARRILEAIELGMMRILRSK 162
             +  ++  QIQ QQQ ++DR ++Q  EK+RIE+E R++ Q R +  A++  + + L+ K
Sbjct: 109 -LINVELVSQIQNQQQSEIDRFVAQQTEKLRIEIEARQQTQTRMLASAVQNVIAKKLKEK 168

Query: 163 EEEIEKIGKLNWELEERVNSLCMENQIWRDVAQTNEATANALRSDLEQVLLQVKTEDPST 222
           ++EI +I  LNW L+ERV SL +ENQIWRD+AQTNEA AN LR++L+QVL Q++T     
Sbjct: 169 DDEIVRIRNLNWVLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQVLAQLET---FP 228

Query: 223 TAAAALADDAESCCGS--NDEGRWRNSDEVEEWELERKRGRKTMRKGKEDRRCKNCWKEE 282
           TA+A + DDAES CGS   D G                 G      G     CK C + E
Sbjct: 229 TASAVVEDDAESSCGSCCGDGG-----------------GEAVTAVG---GGCKRCGERE 288

Query: 283 SCVLLLPCRHLCLCTVCGSS--LHTCPICNSTNNASVRVIM 314
           + VL+LPCRHLCLCTVCG S  L TCP+C+   NASV V M
Sbjct: 289 ASVLVLPCRHLCLCTVCGGSALLRTCPVCDMVMNASVHVNM 302

BLAST of Lcy07g013630 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.2e-39
Identity = 100/209 (47.85%), Postives = 130/209 (62.20%), Query Frame = 0

Query: 107 QIQQQQFDLDRLISQHMEKVRIEVEERRKRQARRILEAIELGMMRILRSKEEEIEKIGKL 166
           QIQQQ  ++DR ++Q  E +RIE+E R++ Q R +  A++  +++ L++K+EEI ++GKL
Sbjct: 105 QIQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRMGKL 164

Query: 167 NWELEERVNSLCMENQIWRDVAQTNEATANALRSDLEQVLLQVKTEDPSTTAAAALADDA 226
           NW L+ERV +L +ENQIWRD+AQTNEATAN LRS+LEQVL QV   D         ADDA
Sbjct: 165 NWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRRPLVEEADDA 224

Query: 227 ESCCGSNDEGRWRNSDEVEEWELERKRGRKTMRKGKEDRRCKNCWKEESCVLLLPCRHLC 286
           ES CGS D G                           +  CK C +  + VL+LPCRHLC
Sbjct: 225 ESSCGSCDGGD---------------------VTAVVNGGCKRCGELTASVLVLPCRHLC 284

Query: 287 LCTVCGSS--LHTCPICNSTNNASVRVIM 314
           LCTVCGSS  L TCP+C+    ASV V M
Sbjct: 285 LCTVCGSSALLRTCPVCDMVMTASVHVNM 292

BLAST of Lcy07g013630 vs. ExPASy Swiss-Prot
Match: A9JTP3 (Baculoviral IAP repeat-containing protein 7 OS=Xenopus tropicalis OX=8364 GN=birc7 PE=2 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 2.4e-05
Identity = 23/56 (41.07%), Postives = 33/56 (58.93%), Query Frame = 0

Query: 258 MRKGKEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHTCPICNSTNNASVRVIM 314
           +R+ KE+R CK C   +  ++ +PC HL +CT C  +L  CPIC +    SVR  M
Sbjct: 309 LRQLKEERMCKVCMDNDVSMVFVPCGHLVVCTECAPNLRHCPICRAAIRGSVRAFM 364

BLAST of Lcy07g013630 vs. ExPASy TrEMBL
Match: A0A6J1G785 (probable BOI-related E3 ubiquitin-protein ligase 3 OS=Cucurbita moschata OX=3662 GN=LOC111451502 PE=4 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 6.4e-134
Identity = 271/334 (81.14%), Postives = 285/334 (85.33%), Query Frame = 0

Query: 1   MGYGAPLTGFSANAAAAETTLLPLPLPPYNSVAIDSFPKAAVA-ATATASTAAVMKSDSG 60
           MGYGAPLTGFSANAAAAETTL    LPPYN +AIDSFPKAA A  TATASTAA +KSDSG
Sbjct: 1   MGYGAPLTGFSANAAAAETTL----LPPYNYIAIDSFPKAAAAMTTATASTAAGLKSDSG 60

Query: 61  ITYNLPIPRKRARDHSTINRFVSYPSHAHSQKNCGCNYYFLGEDISIQIQQQQFDLDRLI 120
           ITYNL IPRKRARDHSTINRF SYPS AH QKNCGCN+ FLGEDIS Q  QQQFDLDRLI
Sbjct: 61  ITYNLRIPRKRARDHSTINRFASYPSPAHFQKNCGCNHDFLGEDISDQFHQQQFDLDRLI 120

Query: 121 SQHMEKVRIEVEERRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCM 180
           SQHMEKVR+E+EE+RKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCM
Sbjct: 121 SQHMEKVRMEMEEKRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCM 180

Query: 181 ENQIWRDVAQTNEATANALRSDLEQVLLQVKT--------EDPSTTAAAALADDAESCCG 240
           E QIWRDVAQTNEATANALRS+LEQVL QV+         EDP+TTAA A+ DDAESCCG
Sbjct: 181 ETQIWRDVAQTNEATANALRSNLEQVLSQVRNNDHQGAGFEDPTTTAAVAV-DDAESCCG 240

Query: 241 SN----------DEGRWRN-SDEVEEWELERKRGRKTMRKGKEDRRCKNCWKEESCVLLL 300
           SN          + GRWRN ++EVEE E   +RGRKT RKG EDR CKNCWKEESCVLLL
Sbjct: 241 SNYDDDDDRKQANHGRWRNLNEEVEESEERNRRGRKT-RKGNEDRWCKNCWKEESCVLLL 300

Query: 301 PCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 315
           PCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP
Sbjct: 301 PCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 328

BLAST of Lcy07g013630 vs. ExPASy TrEMBL
Match: A0A6J1I570 (probable BOI-related E3 ubiquitin-protein ligase 3 OS=Cucurbita maxima OX=3661 GN=LOC111469724 PE=4 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 3.0e-131
Identity = 268/337 (79.53%), Postives = 280/337 (83.09%), Query Frame = 0

Query: 1   MGYGAPLTGFSANAAAAETTLLPLPLPPYNSVAIDSFPKAAVAATATASTAAVMKSDSGI 60
           MGYGAP TGFSANAAAAETTL    LPPYN +AIDSFPKA     A ASTAA +KSDSGI
Sbjct: 1   MGYGAPFTGFSANAAAAETTL----LPPYNYIAIDSFPKA-----AAASTAAGLKSDSGI 60

Query: 61  TYNLPIPRKRARDHSTINRFVSYPSHAHSQKNCGCNYYFLGEDISIQIQQQQFDLDRLIS 120
           TYNLPIPRKRARDHSTINRFVSYPS AH QKNCGCN  FLGEDIS Q Q QQFDLDRLIS
Sbjct: 61  TYNLPIPRKRARDHSTINRFVSYPSPAHFQKNCGCNLDFLGEDISNQFQHQQFDLDRLIS 120

Query: 121 QHMEKVRIEVEERRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCME 180
           QHMEKVR+E+EE+RKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCME
Sbjct: 121 QHMEKVRMEMEEKRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCME 180

Query: 181 NQIWRDVAQTNEATANALRSDLEQVLLQVKT--------EDPSTTAAAALADDAESCCGS 240
            QIWRDVAQTNEATANALRS+LEQVL QV+         EDP+TTAA A ADDAESCCGS
Sbjct: 181 TQIWRDVAQTNEATANALRSNLEQVLSQVRNNDHQGAGFEDPTTTAAVA-ADDAESCCGS 240

Query: 241 N--------------DEGRWRN-SDEVEEWELERKRGRKTMRKGKEDRRCKNCWKEESCV 300
           N              + G WRN ++EVEE E   +RGRKT RKG EDR CKNCWKEESCV
Sbjct: 241 NYNDDGDDDDDRKQANHGPWRNLNEEVEESEGRNRRGRKT-RKGNEDRWCKNCWKEESCV 300

Query: 301 LLLPCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 315
           LLLPCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP
Sbjct: 301 LLLPCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 326

BLAST of Lcy07g013630 vs. ExPASy TrEMBL
Match: A0A6J1JCV5 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483871 PE=4 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 4.9e-126
Identity = 253/319 (79.31%), Postives = 274/319 (85.89%), Query Frame = 0

Query: 1   MGYGAPLTGFSANAAAAETTLLPLPLPPYNSVAIDSFPKA--AVAATATASTAAVMKSDS 60
           MGYGAPLTGFSAN AAAETTL    LPPYNSVAIDSFPKA  A+ A+A+AST  VMKSDS
Sbjct: 1   MGYGAPLTGFSANTAAAETTL----LPPYNSVAIDSFPKALGAMPASASASTGVVMKSDS 60

Query: 61  GITYNLPIPRKRARDHSTINRFVSYP-SHAHSQKNCGCNYYFLGEDISIQIQQQQFDLDR 120
           GI+YNLPIPRKR RDHSTINRF+SYP SHAHSQKNCG N+YFLGEDISI +QQQQ DL+R
Sbjct: 61  GISYNLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNFYFLGEDISILVQQQQLDLER 120

Query: 121 LISQHMEKVRIEVEERRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSL 180
           LISQHMEKVR+E+EE+RKR+ARRI+EAIE+GMM ILRSKEEEIEK+GKLNWELEERVNSL
Sbjct: 121 LISQHMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSL 180

Query: 181 CMENQIWRDVAQTNEATANALRSDLEQVLLQVKTEDPSTTAAAALADDAESCCGSNDEGR 240
           CMENQIWRDVA+TNEATANALRSDLE VLL VK E+          + AESCCGSN  G 
Sbjct: 181 CMENQIWRDVAETNEATANALRSDLELVLLDVKKEE-----EVEEEEKAESCCGSN--GG 240

Query: 241 WRNSDEVEEWELERKRGRKTMRKGK--EDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSL 300
               +E EE E ER++GRKT RK K  EDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSL
Sbjct: 241 DMEEEEEEEVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSL 300

Query: 301 HTCPICNSTNNASVRVIMP 315
           H CPICNSTNNASVRVIMP
Sbjct: 301 HHCPICNSTNNASVRVIMP 308

BLAST of Lcy07g013630 vs. ExPASy TrEMBL
Match: A0A6J1FUW1 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448331 PE=4 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 7.1e-125
Identity = 252/319 (79.00%), Postives = 272/319 (85.27%), Query Frame = 0

Query: 1   MGYGAPLTGFSANAAAAETTLLPLPLPPYNSVAIDSFPKA--AVAATATASTAAVMKSDS 60
           MGYGAPLTGFSAN AAAETTL    LPPYNSVAIDSFPKA  A+ A+A+AST  VMKSDS
Sbjct: 1   MGYGAPLTGFSANTAAAETTL----LPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDS 60

Query: 61  GITYNLPIPRKRARDHSTINRFVSYP-SHAHSQKNCGCNYYFLGEDISIQIQQQQFDLDR 120
            I+YNLPIPRKR RDHSTINRF+SYP SHAHSQKNCG N YFLGEDISI +QQQQ DL+R
Sbjct: 61  AISYNLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLER 120

Query: 121 LISQHMEKVRIEVEERRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSL 180
           LISQHMEKVR+E+EE+RKR+ARRI+EAIE+GMM ILRSKEEEIEK+GKLNWELEERVNSL
Sbjct: 121 LISQHMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSL 180

Query: 181 CMENQIWRDVAQTNEATANALRSDLEQVLLQVKTEDPSTTAAAALADDAESCCGSNDEGR 240
           CMENQIWRDVA+TNEATANALRSDLE VLL VK E+          + AESCCGSN  G 
Sbjct: 181 CMENQIWRDVAETNEATANALRSDLELVLLDVKKEE-----EVEEEEKAESCCGSN--GG 240

Query: 241 WRNSDEVEEWELERKRGRKTMRKGK--EDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSL 300
               +E EE E ER++GRKT RK K  EDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSL
Sbjct: 241 DMEEEEEEEVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSL 300

Query: 301 HTCPICNSTNNASVRVIMP 315
           H CPICNSTNNASVRVIMP
Sbjct: 301 HHCPICNSTNNASVRVIMP 308

BLAST of Lcy07g013630 vs. ExPASy TrEMBL
Match: A0A6J1JF86 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111483871 PE=4 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 1.2e-124
Identity = 253/320 (79.06%), Postives = 274/320 (85.62%), Query Frame = 0

Query: 1   MGYGAPLTGFSANAAAAETTLLPLPLPPYNSVAIDSFPKA--AVAATATASTAAVMKSDS 60
           MGYGAPLTGFSAN AAAETTL    LPPYNSVAIDSFPKA  A+ A+A+AST  VMKSDS
Sbjct: 1   MGYGAPLTGFSANTAAAETTL----LPPYNSVAIDSFPKALGAMPASASASTGVVMKSDS 60

Query: 61  GITYNLPIPRKRARDHSTINRFVSYP-SHAHSQKNCGCNYYFLGEDISIQIQQQQFDLDR 120
           GI+YNLPIPRKR RDHSTINRF+SYP SHAHSQKNCG N+YFLGEDISI +QQQQ DL+R
Sbjct: 61  GISYNLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNFYFLGEDISILVQQQQLDLER 120

Query: 121 LISQH-MEKVRIEVEERRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNS 180
           LISQH MEKVR+E+EE+RKR+ARRI+EAIE+GMM ILRSKEEEIEK+GKLNWELEERVNS
Sbjct: 121 LISQHQMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNS 180

Query: 181 LCMENQIWRDVAQTNEATANALRSDLEQVLLQVKTEDPSTTAAAALADDAESCCGSNDEG 240
           LCMENQIWRDVA+TNEATANALRSDLE VLL VK E+          + AESCCGSN  G
Sbjct: 181 LCMENQIWRDVAETNEATANALRSDLELVLLDVKKEE-----EVEEEEKAESCCGSN--G 240

Query: 241 RWRNSDEVEEWELERKRGRKTMRKGK--EDRRCKNCWKEESCVLLLPCRHLCLCTVCGSS 300
                +E EE E ER++GRKT RK K  EDRRCKNCWKEESCVLLLPCRHLCLCTVCGSS
Sbjct: 241 GDMEEEEEEEVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSS 300

Query: 301 LHTCPICNSTNNASVRVIMP 315
           LH CPICNSTNNASVRVIMP
Sbjct: 301 LHHCPICNSTNNASVRVIMP 309

BLAST of Lcy07g013630 vs. NCBI nr
Match: KAG6604725.1 (putative BOI-related E3 ubiquitin-protein ligase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 488.8 bits (1257), Expect = 3.5e-134
Identity = 272/337 (80.71%), Postives = 286/337 (84.87%), Query Frame = 0

Query: 1   MGYGAPLTGFSANAAAAETTLLPLPLPPYNSVAIDSFPKAAVA-ATATASTAAVMKSDSG 60
           MGYGAPLTGFSANAAAAETTL    LPPYN +AIDSFPKAA A  TATASTAA +KSDSG
Sbjct: 1   MGYGAPLTGFSANAAAAETTL----LPPYNYIAIDSFPKAAAAMTTATASTAAGLKSDSG 60

Query: 61  ITYNLPIPRKRARDHSTINRFVSYPSHAHSQKNCGCNYYFLGEDISIQIQQQQFDLDRLI 120
           ITYNLPIPRKRARDHSTINRF SYPS AH QKNCGCN+ FLGEDIS Q  QQQFDLDRLI
Sbjct: 61  ITYNLPIPRKRARDHSTINRFASYPSPAHFQKNCGCNHDFLGEDISNQFHQQQFDLDRLI 120

Query: 121 SQHMEKVRIEVEERRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCM 180
           SQHMEKVR+E+EE+RKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCM
Sbjct: 121 SQHMEKVRMEMEEKRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCM 180

Query: 181 ENQIWRDVAQTNEATANALRSDLEQVLLQVKT--------EDPSTTAAAALADDAESCCG 240
           E QIWRDVAQTNEATANALRS+LEQVL QV+         EDP+TTAA A+ DDAESCCG
Sbjct: 181 ETQIWRDVAQTNEATANALRSNLEQVLSQVRNNDHQGAGFEDPTTTAAVAV-DDAESCCG 240

Query: 241 SN-------------DEGRWRN-SDEVEEWELERKRGRKTMRKGKEDRRCKNCWKEESCV 300
           SN             + GRWRN ++EVEE E   +RGRKT RKG EDR CKNCWKEESCV
Sbjct: 241 SNYDDDDDDDDRKQANPGRWRNLNEEVEESEERNRRGRKT-RKGNEDRWCKNCWKEESCV 300

Query: 301 LLLPCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 315
           LLLPCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP
Sbjct: 301 LLLPCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 331

BLAST of Lcy07g013630 vs. NCBI nr
Match: XP_022947721.1 (probable BOI-related E3 ubiquitin-protein ligase 3 [Cucurbita moschata])

HSP 1 Score: 486.9 bits (1252), Expect = 1.3e-133
Identity = 271/334 (81.14%), Postives = 285/334 (85.33%), Query Frame = 0

Query: 1   MGYGAPLTGFSANAAAAETTLLPLPLPPYNSVAIDSFPKAAVA-ATATASTAAVMKSDSG 60
           MGYGAPLTGFSANAAAAETTL    LPPYN +AIDSFPKAA A  TATASTAA +KSDSG
Sbjct: 1   MGYGAPLTGFSANAAAAETTL----LPPYNYIAIDSFPKAAAAMTTATASTAAGLKSDSG 60

Query: 61  ITYNLPIPRKRARDHSTINRFVSYPSHAHSQKNCGCNYYFLGEDISIQIQQQQFDLDRLI 120
           ITYNL IPRKRARDHSTINRF SYPS AH QKNCGCN+ FLGEDIS Q  QQQFDLDRLI
Sbjct: 61  ITYNLRIPRKRARDHSTINRFASYPSPAHFQKNCGCNHDFLGEDISDQFHQQQFDLDRLI 120

Query: 121 SQHMEKVRIEVEERRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCM 180
           SQHMEKVR+E+EE+RKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCM
Sbjct: 121 SQHMEKVRMEMEEKRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCM 180

Query: 181 ENQIWRDVAQTNEATANALRSDLEQVLLQVKT--------EDPSTTAAAALADDAESCCG 240
           E QIWRDVAQTNEATANALRS+LEQVL QV+         EDP+TTAA A+ DDAESCCG
Sbjct: 181 ETQIWRDVAQTNEATANALRSNLEQVLSQVRNNDHQGAGFEDPTTTAAVAV-DDAESCCG 240

Query: 241 SN----------DEGRWRN-SDEVEEWELERKRGRKTMRKGKEDRRCKNCWKEESCVLLL 300
           SN          + GRWRN ++EVEE E   +RGRKT RKG EDR CKNCWKEESCVLLL
Sbjct: 241 SNYDDDDDRKQANHGRWRNLNEEVEESEERNRRGRKT-RKGNEDRWCKNCWKEESCVLLL 300

Query: 301 PCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 315
           PCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP
Sbjct: 301 PCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 328

BLAST of Lcy07g013630 vs. NCBI nr
Match: XP_023532102.1 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 484.2 bits (1245), Expect = 8.6e-133
Identity = 270/334 (80.84%), Postives = 283/334 (84.73%), Query Frame = 0

Query: 1   MGYGAPLTGFSANAAAAETTLLPLPLPPYNSVAIDSFPKAAVAATATASTAAVMKSDSGI 60
           MGYGAPLTGFSANAAAAETTL    LPPYN +AIDSFPKA     A ASTAA +KSDSGI
Sbjct: 1   MGYGAPLTGFSANAAAAETTL----LPPYNYIAIDSFPKA-----AAASTAAGLKSDSGI 60

Query: 61  TYNLPIPRKRARDHSTINRFVSYPSHAHSQKNCGCNYYFLGEDISIQIQQQQFDLDRLIS 120
           TYNLPIPRKRARDHSTINRFVS+PS AH QKNCGCN+ FLGEDIS Q QQQQFDLDRLIS
Sbjct: 61  TYNLPIPRKRARDHSTINRFVSHPSPAHFQKNCGCNHDFLGEDISNQFQQQQFDLDRLIS 120

Query: 121 QHMEKVRIEVEERRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCME 180
           QHMEKVR+E+EE+RKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCME
Sbjct: 121 QHMEKVRMEMEEKRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCME 180

Query: 181 NQIWRDVAQTNEATANALRSDLEQVLLQVKT--------EDPSTTAAAALADDAESCCGS 240
            QIWRDVAQTNEATANALRS+LEQVL QVK         EDP+TTAA A ADDAESCCGS
Sbjct: 181 TQIWRDVAQTNEATANALRSNLEQVLSQVKNNDHQGAGFEDPTTTAAVA-ADDAESCCGS 240

Query: 241 N-----------DEGRWRN-SDEVEEWELERKRGRKTMRKGKEDRRCKNCWKEESCVLLL 300
           N           + GRWRN ++EVEE E   +RGRK  RKG EDR CKNCWKEESCVLLL
Sbjct: 241 NYDDDDDDRKQANHGRWRNLNEEVEESEERNRRGRKA-RKGNEDRWCKNCWKEESCVLLL 300

Query: 301 PCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 315
           PCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP
Sbjct: 301 PCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 323

BLAST of Lcy07g013630 vs. NCBI nr
Match: KAG7034854.1 (putative BOI-related E3 ubiquitin-protein ligase 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 482.3 bits (1240), Expect = 3.3e-132
Identity = 271/335 (80.90%), Postives = 285/335 (85.07%), Query Frame = 0

Query: 1   MGYGAPLTGFSANAAAAETTLLPLPLPPYNSVAIDSFPKAAVA-ATATASTAAVMKSDSG 60
           MGYGAPLTGFSANAAAAETTL    LPPYN +AIDSFPKAA A  TATASTAA +KSDSG
Sbjct: 1   MGYGAPLTGFSANAAAAETTL----LPPYNYIAIDSFPKAAAAMTTATASTAAGLKSDSG 60

Query: 61  ITYNLPIPRKRARDHSTINRFVSYPSHAHSQKNCGCNYYFLGEDISIQIQQQQFDLDRLI 120
           ITYNL IPRKRARDHSTINRF SYPS AH QKNCGCN+ FLGEDIS Q  QQQFDLDRLI
Sbjct: 61  ITYNLRIPRKRARDHSTINRFASYPSPAHFQKNCGCNHDFLGEDISDQFHQQQFDLDRLI 120

Query: 121 SQH-MEKVRIEVEERRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLC 180
           SQH MEKVR+E+EE+RKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLC
Sbjct: 121 SQHQMEKVRMEMEEKRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLC 180

Query: 181 MENQIWRDVAQTNEATANALRSDLEQVLLQVKT--------EDPSTTAAAALADDAESCC 240
           ME QIWRDVAQTNEATANALRS+LEQVL QV+         EDP+TTAA A+ DDAESCC
Sbjct: 181 METQIWRDVAQTNEATANALRSNLEQVLSQVRNNDHQGAGFEDPTTTAAVAV-DDAESCC 240

Query: 241 GSN----------DEGRWRN-SDEVEEWELERKRGRKTMRKGKEDRRCKNCWKEESCVLL 300
           GSN          + GRWRN ++EVEE E   +RGRKT RKG EDR CKNCWKEESCVLL
Sbjct: 241 GSNYDDDDDRKQANHGRWRNLNEEVEESEERNRRGRKT-RKGNEDRWCKNCWKEESCVLL 300

Query: 301 LPCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 315
           LPCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP
Sbjct: 301 LPCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 329

BLAST of Lcy07g013630 vs. NCBI nr
Match: XP_023532101.1 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 479.6 bits (1233), Expect = 2.1e-131
Identity = 270/335 (80.60%), Postives = 283/335 (84.48%), Query Frame = 0

Query: 1   MGYGAPLTGFSANAAAAETTLLPLPLPPYNSVAIDSFPKAAVAATATASTAAVMKSDSGI 60
           MGYGAPLTGFSANAAAAETTL    LPPYN +AIDSFPKA     A ASTAA +KSDSGI
Sbjct: 1   MGYGAPLTGFSANAAAAETTL----LPPYNYIAIDSFPKA-----AAASTAAGLKSDSGI 60

Query: 61  TYNLPIPRKRARDHSTINRFVSYPSHAHSQKNCGCNYYFLGEDISIQIQQQQFDLDRLIS 120
           TYNLPIPRKRARDHSTINRFVS+PS AH QKNCGCN+ FLGEDIS Q QQQQFDLDRLIS
Sbjct: 61  TYNLPIPRKRARDHSTINRFVSHPSPAHFQKNCGCNHDFLGEDISNQFQQQQFDLDRLIS 120

Query: 121 QH-MEKVRIEVEERRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCM 180
           QH MEKVR+E+EE+RKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCM
Sbjct: 121 QHQMEKVRMEMEEKRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEERVNSLCM 180

Query: 181 ENQIWRDVAQTNEATANALRSDLEQVLLQVKT--------EDPSTTAAAALADDAESCCG 240
           E QIWRDVAQTNEATANALRS+LEQVL QVK         EDP+TTAA A ADDAESCCG
Sbjct: 181 ETQIWRDVAQTNEATANALRSNLEQVLSQVKNNDHQGAGFEDPTTTAAVA-ADDAESCCG 240

Query: 241 SN-----------DEGRWRN-SDEVEEWELERKRGRKTMRKGKEDRRCKNCWKEESCVLL 300
           SN           + GRWRN ++EVEE E   +RGRK  RKG EDR CKNCWKEESCVLL
Sbjct: 241 SNYDDDDDDRKQANHGRWRNLNEEVEESEERNRRGRKA-RKGNEDRWCKNCWKEESCVLL 300

Query: 301 LPCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 315
           LPCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP
Sbjct: 301 LPCRHLCLCTVCGSSLHTCPICNSTNNASVRVIMP 324

BLAST of Lcy07g013630 vs. TAIR 10
Match: AT3G12920.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 235.7 bits (600), Expect = 5.0e-62
Identity = 142/292 (48.63%), Postives = 195/292 (66.78%), Query Frame = 0

Query: 27  PPYNSVAIDSFPKAAVAAT-ATASTAAVMKSDSGITYNLPI-PRKRARDHSTINRFVSYP 86
           P YN   +D     ++  T  +  ++    SD+ + +  P+  RKR+R+ S +   ++  
Sbjct: 54  PIYNISPVDRLVHQSMKPTIQSVDSSLTFNSDNNVDFLRPVSSRKRSREESVV---LNPS 113

Query: 87  SHAHSQKNCGCNYYFLGEDISIQIQQQQFDLDRLISQHMEKVRIEVEERRKRQARRILEA 146
           ++   QKN      FLG+D+S  +QQ  FD+DRLIS H+E++R+E+EE+RK Q RRI+EA
Sbjct: 114 AYMQIQKNPTDPLMFLGQDLSSNVQQHHFDIDRLISNHVERMRMEIEEKRKTQGRRIVEA 173

Query: 147 IELGMMRILRSKEEEIEKIGKLNWELEERVNSLCMENQIWRDVAQTNEATANALRSDLEQ 206
           +E G+M+ LR+K++EI  IGKLN  LEE+V SLC+ENQIWRDVAQ+NEAT NALRS+L+Q
Sbjct: 174 VEQGLMKTLRAKDDEINHIGKLNLFLEEKVKSLCVENQIWRDVAQSNEATVNALRSNLQQ 233

Query: 207 VLLQV---KTEDPSTTAAAALADDAESCCGSNDEGRWRNSDEVEEWEL--ERKRGRKTMR 266
           VL  V   + E+P T     +ADDA+SCCGSNDEG      E E W+L  E +  +K  R
Sbjct: 234 VLAAVERNRWEEPPT-----VADDAQSCCGSNDEG----DSEEERWKLAGEAQDTKKMCR 293

Query: 267 KGKEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHTCPICNSTNNASVRV 312
            G     C++C K E+ VLLLPCRH+CLC+VCGSSL+TCPIC S   AS+ V
Sbjct: 294 VGMS--MCRSCGKGEASVLLLPCRHMCLCSVCGSSLNTCPICKSPKTASLHV 331

BLAST of Lcy07g013630 vs. TAIR 10
Match: AT1G79110.2 (zinc ion binding )

HSP 1 Score: 215.3 bits (547), Expect = 7.0e-56
Identity = 146/323 (45.20%), Postives = 188/323 (58.20%), Query Frame = 0

Query: 9   GFSANAAAAETTLLPLPLPPYNSVAIDSFPKA--AVAATATASTAAVMKSDSGITYN--- 68
           GF  N       + P  +P + S    + P        + T  T A   +D     +   
Sbjct: 34  GFCNNNQTGYGVVSPFSVPNHTSTTTTATPPLLHVYGGSDTIPTTAGYYADGATNLDCEF 93

Query: 69  LPIP-RKRARDHSTIN--RFVSYPSHAHSQKNCGCN------YYFLGEDISI--QIQQQQ 128
            P+P RKR+RD S  N    +     + S  N          + FLG+DI I   + QQQ
Sbjct: 94  FPLPTRKRSRDSSRSNYHHLLLQNPRSSSCVNAATTTTTTTLFSFLGQDIDISSHMNQQQ 153

Query: 129 FDLDRLISQHMEKVRIEVEERRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWELEE 188
            ++DR +S HME+V+ E+EE+RKRQAR I+EAIE G+++ LR KEEE E+IGK+N  LEE
Sbjct: 154 HEIDRFVSLHMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHALEE 213

Query: 189 RVNSLCMENQIWRDVAQTNEATANALRSDLEQVLLQVKTEDPSTTAAAAL--ADDAESCC 248
           RV SL +ENQIWRD+AQTNEATAN LR++LE VL QVK           +   DDAESCC
Sbjct: 214 RVKSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEKNMNEEDDAESCC 273

Query: 249 GSNDEGRWRNSDEVEEWELERKRGRKTMRKGKEDRRCKNCWKEESCVLLLPCRHLCLCTV 308
           GS+  G     +E     +  +R  +   + +  R C+NC +EESCVLLLPCRHLCLC V
Sbjct: 274 GSSCGG---GGEETVRRRVGLEREAQDKAERRRRRMCRNCGEEESCVLLLPCRHLCLCGV 333

Query: 309 CGSSLHTCPICNSTNNASVRVIM 314
           CGSS+HTCPIC S  NASV V M
Sbjct: 334 CGSSVHTCPICTSPKNASVHVNM 353

BLAST of Lcy07g013630 vs. TAIR 10
Match: AT1G79110.1 (zinc ion binding )

HSP 1 Score: 209.9 bits (533), Expect = 2.9e-54
Identity = 146/326 (44.79%), Postives = 188/326 (57.67%), Query Frame = 0

Query: 9   GFSANAAAAETTLLPLPLPPYNSVAIDSFPKA--AVAATATASTAAVMKSDSGITYN--- 68
           GF  N       + P  +P + S    + P        + T  T A   +D     +   
Sbjct: 34  GFCNNNQTGYGVVSPFSVPNHTSTTTTATPPLLHVYGGSDTIPTTAGYYADGATNLDCEF 93

Query: 69  LPIP-RKRARDHSTIN--RFVSYPSHAHSQKNCGCN------YYFLGEDISI--QIQQQQ 128
            P+P RKR+RD S  N    +     + S  N          + FLG+DI I   + QQQ
Sbjct: 94  FPLPTRKRSRDSSRSNYHHLLLQNPRSSSCVNAATTTTTTTLFSFLGQDIDISSHMNQQQ 153

Query: 129 FDLDRLISQH---MEKVRIEVEERRKRQARRILEAIELGMMRILRSKEEEIEKIGKLNWE 188
            ++DR +S H   ME+V+ E+EE+RKRQAR I+EAIE G+++ LR KEEE E+IGK+N  
Sbjct: 154 HEIDRFVSLHLYQMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHA 213

Query: 189 LEERVNSLCMENQIWRDVAQTNEATANALRSDLEQVLLQVKTEDPSTTAAAAL--ADDAE 248
           LEERV SL +ENQIWRD+AQTNEATAN LR++LE VL QVK           +   DDAE
Sbjct: 214 LEERVKSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEKNMNEEDDAE 273

Query: 249 SCCGSNDEGRWRNSDEVEEWELERKRGRKTMRKGKEDRRCKNCWKEESCVLLLPCRHLCL 308
           SCCGS+  G     +E     +  +R  +   + +  R C+NC +EESCVLLLPCRHLCL
Sbjct: 274 SCCGSSCGG---GGEETVRRRVGLEREAQDKAERRRRRMCRNCGEEESCVLLLPCRHLCL 333

Query: 309 CTVCGSSLHTCPICNSTNNASVRVIM 314
           C VCGSS+HTCPIC S  NASV V M
Sbjct: 334 CGVCGSSVHTCPICTSPKNASVHVNM 356

BLAST of Lcy07g013630 vs. TAIR 10
Match: AT4G19700.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 169.5 bits (428), Expect = 4.4e-42
Identity = 120/281 (42.70%), Postives = 162/281 (57.65%), Query Frame = 0

Query: 43  AATATASTAAVMKSDSGITYNLPIP---RKRARD--HSTINRFVSYPSHAHSQKNCGCNY 102
           A    A+ A   K++SG++YN  +P    KR RD   S  N  V   S A    +     
Sbjct: 49  AVEPLAAKANFNKAESGLSYNFTVPPLSTKRQRDFQFSDSNAPVKRRSVAFDSSSPS--- 108

Query: 103 YFLGEDISIQIQ-QQQFDLDRLISQHMEKVRIEVEERRKRQARRILEAIELGMMRILRSK 162
             +  ++  QIQ QQQ ++DR ++Q  EK+RIE+E R++ Q R +  A++  + + L+ K
Sbjct: 109 -LINVELVSQIQNQQQSEIDRFVAQQTEKLRIEIEARQQTQTRMLASAVQNVIAKKLKEK 168

Query: 163 EEEIEKIGKLNWELEERVNSLCMENQIWRDVAQTNEATANALRSDLEQVLLQVKTEDPST 222
           ++EI +I  LNW L+ERV SL +ENQIWRD+AQTNEA AN LR++L+QVL Q++T     
Sbjct: 169 DDEIVRIRNLNWVLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQVLAQLET---FP 228

Query: 223 TAAAALADDAESCCGS--NDEGRWRNSDEVEEWELERKRGRKTMRKGKEDRRCKNCWKEE 282
           TA+A + DDAES CGS   D G                 G      G     CK C + E
Sbjct: 229 TASAVVEDDAESSCGSCCGDGG-----------------GEAVTAVG---GGCKRCGERE 288

Query: 283 SCVLLLPCRHLCLCTVCGSS--LHTCPICNSTNNASVRVIM 314
           + VL+LPCRHLCLCTVCG S  L TCP+C+   NASV V M
Sbjct: 289 ASVLVLPCRHLCLCTVCGGSALLRTCPVCDMVMNASVHVNM 302

BLAST of Lcy07g013630 vs. TAIR 10
Match: AT5G45100.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 165.2 bits (417), Expect = 8.3e-41
Identity = 100/209 (47.85%), Postives = 130/209 (62.20%), Query Frame = 0

Query: 107 QIQQQQFDLDRLISQHMEKVRIEVEERRKRQARRILEAIELGMMRILRSKEEEIEKIGKL 166
           QIQQQ  ++DR ++Q  E +RIE+E R++ Q R +  A++  +++ L++K+EEI ++GKL
Sbjct: 105 QIQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRMGKL 164

Query: 167 NWELEERVNSLCMENQIWRDVAQTNEATANALRSDLEQVLLQVKTEDPSTTAAAALADDA 226
           NW L+ERV +L +ENQIWRD+AQTNEATAN LRS+LEQVL QV   D         ADDA
Sbjct: 165 NWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRRPLVEEADDA 224

Query: 227 ESCCGSNDEGRWRNSDEVEEWELERKRGRKTMRKGKEDRRCKNCWKEESCVLLLPCRHLC 286
           ES CGS D G                           +  CK C +  + VL+LPCRHLC
Sbjct: 225 ESSCGSCDGGD---------------------VTAVVNGGCKRCGELTASVLVLPCRHLC 284

Query: 287 LCTVCGSS--LHTCPICNSTNNASVRVIM 314
           LCTVCGSS  L TCP+C+    ASV V M
Sbjct: 285 LCTVCGSSALLRTCPVCDMVMTASVHVNM 292

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LDD17.0e-6148.63Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37... [more]
F4IDI64.1e-5344.79Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37... [more]
O818516.2e-4142.70E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1[more]
Q9FHE41.2e-3947.85BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG... [more]
A9JTP32.4e-0541.07Baculoviral IAP repeat-containing protein 7 OS=Xenopus tropicalis OX=8364 GN=bir... [more]
Match NameE-valueIdentityDescription
A0A6J1G7856.4e-13481.14probable BOI-related E3 ubiquitin-protein ligase 3 OS=Cucurbita moschata OX=3662... [more]
A0A6J1I5703.0e-13179.53probable BOI-related E3 ubiquitin-protein ligase 3 OS=Cucurbita maxima OX=3661 G... [more]
A0A6J1JCV54.9e-12679.31probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita maxim... [more]
A0A6J1FUW17.1e-12579.00probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita mosch... [more]
A0A6J1JF861.2e-12479.06probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucurbita maxim... [more]
Match NameE-valueIdentityDescription
KAG6604725.13.5e-13480.71putative BOI-related E3 ubiquitin-protein ligase 3, partial [Cucurbita argyrospe... [more]
XP_022947721.11.3e-13381.14probable BOI-related E3 ubiquitin-protein ligase 3 [Cucurbita moschata][more]
XP_023532102.18.6e-13380.84probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 [Cucurbita pepo su... [more]
KAG7034854.13.3e-13280.90putative BOI-related E3 ubiquitin-protein ligase 3 [Cucurbita argyrosperma subsp... [more]
XP_023532101.12.1e-13180.60probable BOI-related E3 ubiquitin-protein ligase 3 isoform X1 [Cucurbita pepo su... [more]
Match NameE-valueIdentityDescription
AT3G12920.15.0e-6248.63SBP (S-ribonuclease binding protein) family protein [more]
AT1G79110.27.0e-5645.20zinc ion binding [more]
AT1G79110.12.9e-5444.79zinc ion binding [more]
AT4G19700.14.4e-4242.70SBP (S-ribonuclease binding protein) family protein [more]
AT5G45100.18.3e-4147.85SBP (S-ribonuclease binding protein) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 153..173
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 263..303
e-value: 3.3E-8
score: 33.3
NoneNo IPR availablePANTHERPTHR42647:SF12BOI-RELATED E3 UBIQUITIN-PROTEIN LIGASE 2-RELATEDcoord: 28..313
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 242..314
e-value: 7.4E-13
score: 49.7
IPR017066E3 ubiquitin-protein ligase BOI-likePANTHERPTHR42647SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEINcoord: 28..313
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 267..302
score: 9.081335

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy07g013630.1Lcy07g013630.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043067 regulation of programmed cell death
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004842 ubiquitin-protein transferase activity