Lcy07g012510 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy07g012510
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Auxin-responsive GH3 family protein
Location	Chr07: 41682293 .. 41685584 (-)
RNA-Seq Expression	Lcy07g012510
Synteny	Lcy07g012510

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAGAGAGAGAGAGAGATTTGTATAATTAGTTTGGTGGGTTGTGAAGAGGGGGAAAGGGCAGTATGTTTTGTGGGCGTTCATTATCGAAGGGCATCTGGTTATAATGGAGAGCGATGAAAAAGGAGAAGTCGGAGGAGGAGGAGTGAGGAATAATGATGAAGAGATGATTGATTGGTTCGAGCAAGTGTCGTCAGAGGCAGGCGTGGCTCAATCCCGCACTCTCCGTGAGATTCTCCGAGAGAATTGTGGGGTGGAGTACCTCAAGCAATGGCTTGGAGATGTCAATATCCATGAAATCACCGACGACTTTGCATTGGAATCTCTCTTCACTTCTCTAGTCCCCCTCTCTTCCCACGCCGATTTGGAGCCTTATCTTCAACGAATCGCCGATGGTGACTCCGCTCCTTTGCTTACTCAACAACCCATCAACACTCTCTCTTTAAGGTACCATGCTCCATTCACTTAATTAAAATAAAAGAATAAGTTTTTATTTTCCCGCCCTTTTGCTTGTTGAATGGTGCGTTCGACTTCAACTAGTCTACGGCCTAATTTATATCCTGCTGCAGTTCTGGCACCACGGAAGGCAGACAAAAGTACGTACCTTTTACTCCCCATAGTGCACAGACTACTCTTCTTATCTTTCGCTTAGCCGCTGCTTACAGATCCAGGTACCAACGCACAACAATCAACTTGATGATCGATTTATGGCCTTTTCTTTTTTTAAAAAAAAAAAATAATTATTATTCTCTCTCTCTCTCTCTCTATCTCTCAATCAACTTGTCTAAACTAGGGTTTATCCCACAAGGGAAGGAGGGAAGATTCTAGAGTTCATTTACAGCAGCAAACAGACCAAGACCAAAGGAGGAATAACGACCGGGACCGCCACGACCCATTACTACGCCAGCGAACAATTCAAAATCAAGCAGCTCGAAACCAAGTCCTTCACATGCAGCCCACAAGAAGTCATTTCCGGCGGTGACTACAAGCAATCCACATACTGCCATCTACTTCTCGGCCTATTTTTCAGCGACCAAGTCGAGTACGTCACCTCCACTTTCGCCTACAGCATAGTCCAGGCCTTCAACGAGCTCGAAGAGGCTTGGCCCGAGATCTGCCACGACATAACCCACGGCACGCTCAGCTCGAGAATCACTCTCCCGAAGATCCGGAAGGCTGTTCTGAACGTGATCCGTCCGAACCCAGGTCTGGCGGCGGAGATACGGGGGCAGTGCGAGGAGCTGGAAGGGGAGGATTGGTTGGGGTTGATTCCGAAGCTGTGGCGGAATTGCAAGTATGTGTACTCGATAATGACGGGGTCCATGCAGCCGTACTTGAAGAAATTGAGACACTACGCGGGGGGGTTGGCGTTGGTGAGTGCGGATTACGGGTCGACGGAGAGTTGGATTGGGGTTAATGTGGAGCCTGGTTTACCACCCGAAAATGTTACTTTTGCGGTGATTCCCACTTTCGCTTATTTTGAGTTCATCCCACTTTTCAGACAACACCAACAACAACAACATTATTGTTGCGGTTCTTCCGATATTGCCACTGATGATTTCTTGGAAGGCCAACCGGTGCCCTTGTCTCAGGTCAAGATTGGACAAGAGTATGAACTTGTTCTTACTACTTTCACAGGTCAGTTGTCTTTTCAATTCCCATACCCGAACATAAAATATCTACATTTCCATTTGTCTCCTTTTTAATTGTGTGTCTTTTCGCTGCCTAATCTGGCCTTTCCATTTCCCTTTAATTAATCGCTTTTCTTCTTCTTCTTTTTTTCCCCCCCATTAATGTGGATCTGTTTCCTTCTACTTTTTTGTCCACAAAAATCTAATAAAGCATTGGTTTTCCTAGTACATTATATGATTGTGTCCATCACTACAAAATATCTCTCGACAAGACGATAATTAGTATTATTATTGTATGGTAATGATGGATATATCAAGTAATTGATAAATTAATGTGTCACTACTCTCAATTTTCTTCTCTGGCCCCCATAACTTCCAGCCTGCCTTATTTTCTAAATTAAATAATTAGACCATGGTGAGTTTAATCATTTGAATGTATTCTGGATATTAACAGTAACATATTTGTCTTCTGATTTTCATGGATATCAACGTACCCCACTAAGGGGTTCCATATGTTGTAGCTAGATTGATTGTCATATTAACCAAAAGAATCCAATTTTTGCAAATTAGTGAGCTTTAGAAGATTCATCAATATATGATCAATCAGATCCATACATTGATTTTTCAAAGCTAAAGTTTTGTGCATTGATTGATTTATCCGGGCTTCCTTCTATATGTTAGATTACATCAAATATAAGAGCTAGAGAGATTAATCTAATCGTATTTCAATTAGTTGTAACATATATGTCAGATTGATGTGAGATGTTTTTTGACAAACCGAAGAAATCTTAGGTTTATTCCATGTGTGTTTGTTATTGAATTGGCAGGACTTTATAGATGCAGATTAGGAGACGTAGTAGAAGTAGCGGGGTTTTACAACAAGACGCCGAAACTAAACTTTATATGCAGAAGAAAGCTTGTATTAACCATAAACATTGACAAAAACACGGAGAAGGATCTGCAATTGGTGGTTGAGAGAGGGTCCCAGCTACTAGCCGACCAAAGTGGAGCCGAATTAGTTGACTTCACAAGCCACGCCGACCTGGCCGACCAACCAGGCCACTACGTCATCTTCTGGGAAGTCAAAGGACATGTCGACGACACACTTCTTGGAAAATGCTGCTCGGAAATGGACGCTGCTTTCGTTGACTATGGCTACGTCGTCTCCAGAAGGTCCAATTCCATTGGACCGCTGGAGCTCCGGATTGTGGAGAGAGGCACTTTCAATAAGATTTTGGAACATTATATTGGAAATGGAGCTGCGCTTAGCCAATTCAAGACGCCCAGATGCACTAGAAATCAGGTTCTTCTTAACATTCTCAATCTCTCTACCATCAAAACCTTTCGCAGCACCGCATACGCCTGAGTTTTTTCTTTTGCTCATTGTTGTTCTAGTCTTTGCCAATTTTGAATCGGTTTCGGATCGGAGGTTTCATTTTTTTCTCTTTAATTTCTTTCTTCAAATCCAAAAAACACAAACCCCATAATTGATTCTAACGAAATTAGTTTGTTTAGTGTTTCAATAAAAATCATTTACACTCCATTTGTATCATCATCGTTTCTAAAGTTGTGCCAGAGTTCTACTTTACTATATTCATTCCTTCATTATTATCTACTATCATTCAAGTTACAAGTTTAGTCCCTAAATTTTAGTTTGTGTCTA

mRNA sequence

AGAGAGAGAGAGAGAGATTTGTATAATTAGTTTGGTGGGTTGTGAAGAGGGGGAAAGGGCAGTATGTTTTGTGGGCGTTCATTATCGAAGGGCATCTGGTTATAATGGAGAGCGATGAAAAAGGAGAAGTCGGAGGAGGAGGAGTGAGGAATAATGATGAAGAGATGATTGATTGGTTCGAGCAAGTGTCGTCAGAGGCAGGCGTGGCTCAATCCCGCACTCTCCGTGAGATTCTCCGAGAGAATTGTGGGGTGGAGTACCTCAAGCAATGGCTTGGAGATGTCAATATCCATGAAATCACCGACGACTTTGCATTGGAATCTCTCTTCACTTCTCTAGTCCCCCTCTCTTCCCACGCCGATTTGGAGCCTTATCTTCAACGAATCGCCGATGGTGACTCCGCTCCTTTGCTTACTCAACAACCCATCAACACTCTCTCTTTAAGTTCTGGCACCACGGAAGGCAGACAAAAGTACGTACCTTTTACTCCCCATAGTGCACAGACTACTCTTCTTATCTTTCGCTTAGCCGCTGCTTACAGATCCAGGGTTTATCCCACAAGGGAAGGAGGGAAGATTCTAGAGTTCATTTACAGCAGCAAACAGACCAAGACCAAAGGAGGAATAACGACCGGGACCGCCACGACCCATTACTACGCCAGCGAACAATTCAAAATCAAGCAGCTCGAAACCAAGTCCTTCACATGCAGCCCACAAGAAGTCATTTCCGGCGGTGACTACAAGCAATCCACATACTGCCATCTACTTCTCGGCCTATTTTTCAGCGACCAAGTCGAGTACGTCACCTCCACTTTCGCCTACAGCATAGTCCAGGCCTTCAACGAGCTCGAAGAGGCTTGGCCCGAGATCTGCCACGACATAACCCACGGCACGCTCAGCTCGAGAATCACTCTCCCGAAGATCCGGAAGGCTGTTCTGAACGTGATCCGTCCGAACCCAGGTCTGGCGGCGGAGATACGGGGGCAGTGCGAGGAGCTGGAAGGGGAGGATTGGTTGGGGTTGATTCCGAAGCTGTGGCGGAATTGCAAGTATGTGTACTCGATAATGACGGGGTCCATGCAGCCGTACTTGAAGAAATTGAGACACTACGCGGGGGGGTTGGCGTTGGTGAGTGCGGATTACGGGTCGACGGAGAGTTGGATTGGGGTTAATGTGGAGCCTGGTTTACCACCCGAAAATGTTACTTTTGCGGTGATTCCCACTTTCGCTTATTTTGAGTTCATCCCACTTTTCAGACAACACCAACAACAACAACATTATTGTTGCGGTTCTTCCGATATTGCCACTGATGATTTCTTGGAAGGCCAACCGGTGCCCTTGTCTCAGGTCAAGATTGGACAAGAGTATGAACTTGTTCTTACTACTTTCACAGGACTTTATAGATGCAGATTAGGAGACGTAGTAGAAGTAGCGGGGTTTTACAACAAGACGCCGAAACTAAACTTTATATGCAGAAGAAAGCTTGTATTAACCATAAACATTGACAAAAACACGGAGAAGGATCTGCAATTGGTGGTTGAGAGAGGGTCCCAGCTACTAGCCGACCAAAGTGGAGCCGAATTAGTTGACTTCACAAGCCACGCCGACCTGGCCGACCAACCAGGCCACTACGTCATCTTCTGGGAAGTCAAAGGACATGTCGACGACACACTTCTTGGAAAATGCTGCTCGGAAATGGACGCTGCTTTCGTTGACTATGGCTACGTCGTCTCCAGAAGGTCCAATTCCATTGGACCGCTGGAGCTCCGGATTGTGGAGAGAGGCACTTTCAATAAGATTTTGGAACATTATATTGGAAATGGAGCTGCGCTTAGCCAATTCAAGACGCCCAGATGCACTAGAAATCAGGTTCTTCTTAACATTCTCAATCTCTCTACCATCAAAACCTTTCGCAGCACCGCATACGCCTGAGTTTTTTCTTTTGCTCATTGTTGTTCTAGTCTTTGCCAATTTTGAATCGGTTTCGGATCGGAGGTTTCATTTTTTTCTCTTTAATTTCTTTCTTCAAATCCAAAAAACACAAACCCCATAATTGATTCTAACGAAATTAGTTTGTTTAGTGTTTCAATAAAAATCATTTACACTCCATTTGTATCATCATCGTTTCTAAAGTTGTGCCAGAGTTCTACTTTACTATATTCATTCCTTCATTATTATCTACTATCATTCAAGTTACAAGTTTAGTCCCTAAATTTTAGTTTGTGTCTA

Coding sequence (CDS)

ATGGAGAGCGATGAAAAAGGAGAAGTCGGAGGAGGAGGAGTGAGGAATAATGATGAAGAGATGATTGATTGGTTCGAGCAAGTGTCGTCAGAGGCAGGCGTGGCTCAATCCCGCACTCTCCGTGAGATTCTCCGAGAGAATTGTGGGGTGGAGTACCTCAAGCAATGGCTTGGAGATGTCAATATCCATGAAATCACCGACGACTTTGCATTGGAATCTCTCTTCACTTCTCTAGTCCCCCTCTCTTCCCACGCCGATTTGGAGCCTTATCTTCAACGAATCGCCGATGGTGACTCCGCTCCTTTGCTTACTCAACAACCCATCAACACTCTCTCTTTAAGTTCTGGCACCACGGAAGGCAGACAAAAGTACGTACCTTTTACTCCCCATAGTGCACAGACTACTCTTCTTATCTTTCGCTTAGCCGCTGCTTACAGATCCAGGGTTTATCCCACAAGGGAAGGAGGGAAGATTCTAGAGTTCATTTACAGCAGCAAACAGACCAAGACCAAAGGAGGAATAACGACCGGGACCGCCACGACCCATTACTACGCCAGCGAACAATTCAAAATCAAGCAGCTCGAAACCAAGTCCTTCACATGCAGCCCACAAGAAGTCATTTCCGGCGGTGACTACAAGCAATCCACATACTGCCATCTACTTCTCGGCCTATTTTTCAGCGACCAAGTCGAGTACGTCACCTCCACTTTCGCCTACAGCATAGTCCAGGCCTTCAACGAGCTCGAAGAGGCTTGGCCCGAGATCTGCCACGACATAACCCACGGCACGCTCAGCTCGAGAATCACTCTCCCGAAGATCCGGAAGGCTGTTCTGAACGTGATCCGTCCGAACCCAGGTCTGGCGGCGGAGATACGGGGGCAGTGCGAGGAGCTGGAAGGGGAGGATTGGTTGGGGTTGATTCCGAAGCTGTGGCGGAATTGCAAGTATGTGTACTCGATAATGACGGGGTCCATGCAGCCGTACTTGAAGAAATTGAGACACTACGCGGGGGGGTTGGCGTTGGTGAGTGCGGATTACGGGTCGACGGAGAGTTGGATTGGGGTTAATGTGGAGCCTGGTTTACCACCCGAAAATGTTACTTTTGCGGTGATTCCCACTTTCGCTTATTTTGAGTTCATCCCACTTTTCAGACAACACCAACAACAACAACATTATTGTTGCGGTTCTTCCGATATTGCCACTGATGATTTCTTGGAAGGCCAACCGGTGCCCTTGTCTCAGGTCAAGATTGGACAAGAGTATGAACTTGTTCTTACTACTTTCACAGGACTTTATAGATGCAGATTAGGAGACGTAGTAGAAGTAGCGGGGTTTTACAACAAGACGCCGAAACTAAACTTTATATGCAGAAGAAAGCTTGTATTAACCATAAACATTGACAAAAACACGGAGAAGGATCTGCAATTGGTGGTTGAGAGAGGGTCCCAGCTACTAGCCGACCAAAGTGGAGCCGAATTAGTTGACTTCACAAGCCACGCCGACCTGGCCGACCAACCAGGCCACTACGTCATCTTCTGGGAAGTCAAAGGACATGTCGACGACACACTTCTTGGAAAATGCTGCTCGGAAATGGACGCTGCTTTCGTTGACTATGGCTACGTCGTCTCCAGAAGGTCCAATTCCATTGGACCGCTGGAGCTCCGGATTGTGGAGAGAGGCACTTTCAATAAGATTTTGGAACATTATATTGGAAATGGAGCTGCGCTTAGCCAATTCAAGACGCCCAGATGCACTAGAAATCAGGTTCTTCTTAACATTCTCAATCTCTCTACCATCAAAACCTTTCGCAGCACCGCATACGCCTGA

Protein sequence

MESDEKGEVGGGGVRNNDEEMIDWFEQVSSEAGVAQSRTLREILRENCGVEYLKQWLGDVNIHEITDDFALESLFTSLVPLSSHADLEPYLQRIADGDSAPLLTQQPINTLSLSSGTTEGRQKYVPFTPHSAQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLETKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEYVTSTFAYSIVQAFNELEEAWPEICHDITHGTLSSRITLPKIRKAVLNVIRPNPGLAAEIRGQCEELEGEDWLGLIPKLWRNCKYVYSIMTGSMQPYLKKLRHYAGGLALVSADYGSTESWIGVNVEPGLPPENVTFAVIPTFAYFEFIPLFRQHQQQQHYCCGSSDIATDDFLEGQPVPLSQVKIGQEYELVLTTFTGLYRCRLGDVVEVAGFYNKTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSHADLADQPGHYVIFWEVKGHVDDTLLGKCCSEMDAAFVDYGYVVSRRSNSIGPLELRIVERGTFNKILEHYIGNGAALSQFKTPRCTRNQVLLNILNLSTIKTFRSTAYA

Homology

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BLAST of Lcy07g012510 vs. ExPASy Swiss-Prot
Match: Q9ZNS2 (Indole-3-acetic acid-amido synthetase GH3.10 OS=Arabidopsis thaliana OX=3702 GN=GH3.10 PE=2 SV=1)

HSP 1 Score: 837.8 bits (2163), Expect = 7.8e-242
Identity = 416/596 (69.80%), Postives = 481/596 (80.70%), Query Frame = 0


Query: 14  VRNNDEEMIDWFEQVSSEAGVAQSRTLREILRENCGVEYLKQWLGDVNIHEITDDFALES 73
           V    +++I WFE VS  A   QS TLR IL  N GVEYL++WLG V++ ++ DD+ LE+
Sbjct: 4   VEAGHDDVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKM-DDYTLET 63

Query: 74  LFTSLVPLSSHADLEPYLQRIADGDSAPLLTQQPINTLSLSSGTTEGRQKYVPFTPHSAQ 133
           LFTSLVP+ SHADL+PY+QRIADG+++PLLTQ+PI  LSLSSGTTEGRQKYVPFT HSAQ
Sbjct: 64  LFTSLVPIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQ 123

Query: 134 TTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 193
           TTL IFRL+AAYRSR YP REGG+ILEFIY+ K+ KT GG+T GTATTHYYASE+FK KQ
Sbjct: 124 TTLQIFRLSAAYRSRFYPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYYASEEFKTKQ 183

Query: 194 LETKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEYVTSTFAYSIVQAFNELEEAWP 253
             TKSFTCSPQEVISGGD+ Q TYCHLLLGL +S QVE+V S F+Y+IVQAF+  EE W 
Sbjct: 184 ETTKSFTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQAFSFFEEIWR 243

Query: 254 EICHDITHGTLSSRITLPKIRKAVLNVIRPNPGLAAEIRGQCEELEGE-DWLGLIPKLWR 313
           EIC DI  G LSSRITLPK+RKAVL +IRPNP LA+ I   C ELE    W GLI KLW 
Sbjct: 244 EICADIKEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLGWFGLISKLWP 303

Query: 314 NCKYVYSIMTGSMQPYLKKLRHYAGGLALVSADYGSTESWIGVNVEPGLPPENVTFAVIP 373
           N K++ SIMTGSM PYL KLRHYAGGL LVSADYGSTESWIGVNV+P LPPE+V+FAVIP
Sbjct: 304 NAKFISSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLPPEDVSFAVIP 363

Query: 374 TFAYFEFIPLFRQHQQQQHYCCGSSDIATD-DFLEGQPVPLSQVKIGQEYELVLTTFTGL 433
           TF+YFEFIPL+R+  Q        SDI  D DF+E +PVPLSQVK+GQEYELVLTTFTGL
Sbjct: 364 TFSYFEFIPLYRRQNQ--------SDICIDGDFVEDKPVPLSQVKLGQEYELVLTTFTGL 423

Query: 434 YRCRLGDVVEVAGFYNKTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGSQLLADQSGA 493
           YR RLGDVVEV  F+  TPKL+FI RRKL+LTINIDKNTEKDLQ VV++ SQLL+  + A
Sbjct: 424 YRYRLGDVVEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQLLSRSTRA 483

Query: 494 ELVDFTSHADLADQPGHYVIFWEVKGHVDDTLLGKCCSEMDAAFVDYGYVVSRRSNSIGP 553
           E+VDFTSHAD+  +PGHYVI+WE++G  DD  L +CC EMD AFVDYGYVVSRR NSIGP
Sbjct: 484 EVVDFTSHADVIARPGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSRRMNSIGP 543

Query: 554 LELRIVERGTFNKILEHYIGNGAALSQFKTPRCTRNQVLLNILNLSTIKTFRSTAY 608
           LELR+VERGTF K+ E  +G    L+QFKTPRCT N V+L+ILN STIK FRS+AY
Sbjct: 544 LELRVVERGTFGKVAERCVGKCGGLNQFKTPRCTTNSVMLDILNDSTIKRFRSSAY 590

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BLAST of Lcy07g012510 vs. ExPASy Swiss-Prot
Match: A0A1J6KGJ9 (Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 PE=1 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 1.1e-176
Identity = 308/591 (52.12%), Postives = 418/591 (70.73%), Query Frame = 0


Query: 19  EEMIDWFEQVSSEAGVAQSRTLREILRENCGVEYLKQWLGDVNIHEITDDFALESLFTSL 78
           EE+I+ FE ++ +AG  Q  TL++IL EN G EYL+QW     ++  TD  +    F + 
Sbjct: 14  EEVIEEFEVLTKDAGKIQEETLQKILEENGGTEYLQQW----GLNGKTDSLS----FKNC 73

Query: 79  VPLSSHADLEPYLQRIADGDSAPLLTQQPINTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 138
           +P+ +H DLEPY+ RIADGD +P+LT +PI T+SLSSGTT+G+ K+VPF     ++T+ I
Sbjct: 74  IPIVTHKDLEPYIHRIADGDLSPILTGKPITTISLSSGTTQGKPKFVPFNEELMESTMQI 133

Query: 139 FRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLETKS 198
           F+ +  +R+R +P    GK L+FIY SKQ KTKGG+  GTATT+ Y + QFK      ++
Sbjct: 134 FKTSFVFRNREFPV-VNGKALQFIYGSKQFKTKGGLAAGTATTNVYRNAQFKKTMKAMQT 193

Query: 199 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEYVTSTFAYSIVQAFNELEEAWPEICHD 258
             CSP EVI G D++QS YCHLL GL F D+V+ V+STFA+SIV AF   E+ W E+  +
Sbjct: 194 PCCSPDEVIFGPDFQQSLYCHLLCGLIFRDEVQVVSSTFAHSIVHAFRNFEQIWQELVTN 253

Query: 259 ITHGTLSSRITLPKIRKAVLNVIRPNPGLAAEIRGQCEELEGEDWLGLIPKLWRNCKYVY 318
           I  G LSSR+ +P +R A+  +++P+P LA  I  +C  L   +W GLIP+L+ N +Y+Y
Sbjct: 254 IREGVLSSRVIVPSMRAAMSKLLKPDPELADTIFNKCSRL--SNWYGLIPELFPNTRYIY 313

Query: 319 SIMTGSMQPYLKKLRHYAGGLALVSADYGSTESWIGVNVEPGLPPENVTFAVIPTFAYFE 378
            IMTGSM+PYLKKLRHYAG L L+SADYGS+E WIG NV P LPPE VT+AV+P   YFE
Sbjct: 314 GIMTGSMEPYLKKLRHYAGDLPLLSADYGSSEGWIGANVNPELPPELVTYAVLPNIGYFE 373

Query: 379 FIPLFRQHQQQQHYCCGSSDIATDDFLEGQPVPLSQVKIGQEYELVLTTFTGLYRCRLGD 438
           FIPL                    D LE  PV L++VK+G+EYE+V+T F GLYR RLGD
Sbjct: 374 FIPLMENL----------------DGLEPMPVGLTEVKLGEEYEIVVTNFAGLYRYRLGD 433

Query: 439 VVEVAGFYNKTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS 498
           VV++ GF+N TP+L FICRR L+L+INIDKNTEKDLQL VE  +++L+D+   E+VDFTS
Sbjct: 434 VVKIKGFHNGTPELQFICRRNLLLSINIDKNTEKDLQLAVEAAAKILSDEK-LEVVDFTS 493

Query: 499 HADLADQPGHYVIFWEVKGHVDDTLLGKCCSEMDAAFVDYGYVVSRRSNSIGPLELRIVE 558
           H +++  PGHYVIFWE+ G   + +L +CC+ +D +FVD GYV SR+ ++IG LELRIV+
Sbjct: 494 HVNVSADPGHYVIFWELNGEASEEILKECCNCLDKSFVDAGYVGSRKVHAIGALELRIVK 553

Query: 559 RGTFNKILEHYIGNGAALSQFKTPRCT--RNQVLLNILNLSTIKTFRSTAY 608
           RGTF+KIL+H++G GAA+SQFKTPRC    N  +L IL+ + ++++ STA+
Sbjct: 554 RGTFHKILDHFVGLGAAVSQFKTPRCVGPTNLSVLQILSSNVVESYFSTAF 576

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BLAST of Lcy07g012510 vs. ExPASy Swiss-Prot
Match: A0A314KSQ4 (Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 PE=1 SV=2)

HSP 1 Score: 615.5 bits (1586), Expect = 6.3e-175
Identity = 310/593 (52.28%), Postives = 413/593 (69.65%), Query Frame = 0


Query: 17  NDEEMIDWFEQVSSEAGVAQSRTLREILRENCGVEYLKQWLGDVNIHEITDDFALESLFT 76
           + E++I+ FE ++ +AG  Q  TL++IL +N G EYL+ W     ++  TD       F 
Sbjct: 13  DQEKVIEEFEDLTKDAGKIQEETLKKILEQNGGTEYLQLW----GLNGRTD----PQTFK 72

Query: 77  SLVPLSSHADLEPYLQRIADGDSAPLLTQQPINTLSLSSGTTEGRQKYVPFTPHSAQTTL 136
           + VP+ +H DLEPY+QRIADGD +P+LT +PI T+SLSSGTT+G+ K+VPF     ++T+
Sbjct: 73  NCVPIVTHNDLEPYIQRIADGDLSPILTGKPIETISLSSGTTQGKPKFVPFNDELMESTM 132

Query: 137 LIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLET 196
            IF+ + A+R+R +P    GK L+FIYSSKQ KTKGG+  GTATT+ Y + QFK      
Sbjct: 133 QIFKTSFAFRNREFPI-GNGKALQFIYSSKQFKTKGGLAAGTATTNVYRNAQFKKTMKAM 192

Query: 197 KSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEYVTSTFAYSIVQAFNELEEAWPEIC 256
            +  CSP EVI G D+ QS YCHLL GL F D+V+ V+STFA+SIV AF   E+ W  + 
Sbjct: 193 CTPCCSPDEVIFGPDFHQSLYCHLLCGLIFHDEVQVVSSTFAHSIVHAFRTFEQVWEALV 252

Query: 257 HDITHGTLSSRITLPKIRKAVLNVIRPNPGLAAEIRGQCEELEGEDWLGLIPKLWRNCKY 316
            DI  G LSSR+T+P IR A+  +++P+P LA  I  +C  L   +W GLIP L+ N +Y
Sbjct: 253 VDIREGVLSSRVTVPSIRLAMSKLLKPDPELADTIYNKCSRL--SNWYGLIPDLFPNTRY 312

Query: 317 VYSIMTGSMQPYLKKLRHYAGGLALVSADYGSTESWIGVNVEPGLPPENVTFAVIPTFAY 376
           +Y IMTGSM+PYLKKLRHYAG L L+SADYGS+E W+GVNV P LPPE VT+AV+P   Y
Sbjct: 313 IYGIMTGSMEPYLKKLRHYAGELPLLSADYGSSEGWVGVNVNPKLPPELVTYAVLPNIGY 372

Query: 377 FEFIPLFRQHQQQQHYCCGSSDIATDDFLEGQPVPLSQVKIGQEYELVLTTFTGLYRCRL 436
           FEFIPL            G +    +      PV L++VK+G+EYE+V T F GLYR RL
Sbjct: 373 FEFIPL------------GGNLNGIEQ--ANSPVGLTEVKLGEEYEVVFTNFAGLYRYRL 432

Query: 437 GDVVEVAGFYNKTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGSQLLADQSGAELVDF 496
           GDVV+V GF+N TP+L F+CR  L+L+INIDKNTEKDLQL VE  ++ L D+   E+VDF
Sbjct: 433 GDVVKVKGFHNGTPELQFVCRSNLLLSINIDKNTEKDLQLAVEAAAKRLVDEK-LEVVDF 492

Query: 497 TSHADLADQPGHYVIFWEVKGHVDDTLLGKCCSEMDAAFVDYGYVVSRRSNSIGPLELRI 556
           TSH +++  PGHYVIFWE+ G   D +L  CC+ +D +F+D GYV SR+ N+IG LELRI
Sbjct: 493 TSHVNVSADPGHYVIFWELSGEATDEMLQDCCNCLDRSFIDAGYVSSRKVNAIGALELRI 552

Query: 557 VERGTFNKILEHYIGNGAALSQFKTPRCT--RNQVLLNILNLSTIKTFRSTAY 608
           V+RGTF+KIL+H++G G A+SQFKTPRC   +N  LL IL+ + ++T+ STA+
Sbjct: 553 VKRGTFHKILDHFVGLGGAVSQFKTPRCVGPKNSSLLQILSSNVVETYVSTAF 579

[Close]

BLAST of Lcy07g012510 vs. ExPASy Swiss-Prot
Match: Q6I581 (Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 1.8e-166
Identity = 292/591 (49.41%), Postives = 400/591 (67.68%), Query Frame = 0


Query: 19  EEMIDWFEQVSSEAGVAQSRTLREILRENCGVEYLKQWLGDVNIHEITDDFALESLFTSL 78
           EE I+ FE ++ +A   Q  TL++IL  N   EYL+ +     +   TD       + S 
Sbjct: 7   EETINEFEMLTRDAARVQKDTLKKILEINASAEYLQNF----GLGGRTD----AESYKSC 66

Query: 79  VPLSSHADLEPYLQRIADGDSAPLLTQQPINTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 138
           +PL  H D+EPY+QRI DGD++P++T +PI  LSLSSGTT G+ K++PF     +TTL I
Sbjct: 67  IPLCVHNDIEPYIQRIVDGDTSPVVTGEPITNLSLSSGTTHGKPKFIPFNDELLETTLQI 126

Query: 139 FRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLETKS 198
           +R + A+R+R YP  + GK L+F+Y SKQ  TKGGI   TATT+ Y  +++K    + +S
Sbjct: 127 YRTSYAFRNREYPIGQ-GKALQFVYGSKQVITKGGILATTATTNLYRRQRYKEGMKDIQS 186

Query: 199 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEYVTSTFAYSIVQAFNELEEAWPEICHD 258
             CSP EVI G D+ QS YCHLL GL +S++V  V STFA+S+V AF   EE W ++C D
Sbjct: 187 QCCSPDEVIFGPDFHQSLYCHLLCGLIYSEEVHSVFSTFAHSLVHAFQTFEEVWEDLCTD 246

Query: 259 ITHGTLSSRITLPKIRKAVLNVIRPNPGLAAEIRGQCEELEGEDWLGLIPKLWRNCKYVY 318
           I  G LS ++T P IR+AV  +++PNP LA  I  +C  L   +W G+IP LW N KYVY
Sbjct: 247 IRDGVLSKKVTAPSIREAVSKILKPNPELADSIYKKCIGL--SNWYGVIPALWPNAKYVY 306

Query: 319 SIMTGSMQPYLKKLRHYAGGLALVSADYGSTESWIGVNVEPGLPPENVTFAVIPTFAYFE 378
            IMTGSM+PYLKKLRHYAG L L+SADYG++E W+G N++P +PPE VT+AV+P   YFE
Sbjct: 307 GIMTGSMEPYLKKLRHYAGNLPLISADYGASEGWVGSNIDPTVPPEQVTYAVLPQVGYFE 366

Query: 379 FIPLFRQHQQQQHYCCGSSDIATDDFLEGQPVPLSQVKIGQEYELVLTTFTGLYRCRLGD 438
           FIPL +   ++      + + A+  ++E  PV L++V++G+ YE+V+T F GLYR RLGD
Sbjct: 367 FIPLEKPIGEE------TENSASIHYIESDPVGLTEVEVGKIYEVVITNFAGLYRYRLGD 426

Query: 439 VVEVAGFYNKTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS 498
           VV++A F+N TP+L FICRR LVL+INIDKNTEKDLQL VE  S+ L  +   E++DFTS
Sbjct: 427 VVKIARFHNSTPELQFICRRSLVLSINIDKNTEKDLQLAVEEASKFLEGEK-LEVMDFTS 486

Query: 499 HADLADQPGHYVIFWEVKGHVDDTLLGKCCSEMDAAFVDYGYVVSRRSNSIGPLELRIVE 558
             + +  PG YVIFWE+ G   D +L  C + +D AF+D GY  SR+  +IGPLELRI+ 
Sbjct: 487 FVERSSDPGRYVIFWELSGDASDEVLSSCANALDLAFIDAGYTGSRKIKTIGPLELRILR 546

Query: 559 RGTFNKILEHYIGNGAALSQFKTPRCTR--NQVLLNILNLSTIKTFRSTAY 608
           +GTF +IL+H++  G A+SQFKTPR     N  +L IL+ +  +++ STAY
Sbjct: 547 KGTFKEILDHFLSLGGAVSQFKTPRFVNPSNSKVLQILSRNVTQSYFSTAY 579

[Close]

BLAST of Lcy07g012510 vs. ExPASy Swiss-Prot
Match: Q9SKE2 (Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 PE=1 SV=2)

HSP 1 Score: 570.5 bits (1469), Expect = 2.3e-161
Identity = 286/589 (48.56%), Postives = 392/589 (66.55%), Query Frame = 0


Query: 21  MIDWFEQVSSEAGVAQSRTLREILRENCGVEYLKQWLGDVNIHEITDDFALESLFTSLVP 80
           +ID F++++  A   Q +TL+EIL +N    YL+    + N    TD    E  F S+VP
Sbjct: 13  VIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGN---ATDP---EEAFKSMVP 72

Query: 81  LSSHADLEPYLQRIADGDSAPLLTQQPINTLSLSSGTTEGRQKYVPFTPHSAQTTLLIFR 140
           L +  +LEPY++R+ DGD++P+LT  P+  +SLSSGT++GR K++PFT    + TL +FR
Sbjct: 73  LVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLFR 132

Query: 141 LAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLETKSFT 200
            A A+R+R +P  + GK L+FI+SSKQ  + GG+  GTATT+ Y +  FK       S +
Sbjct: 133 TAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSPS 192

Query: 201 CSPQEVISGGDYKQSTYCHLLLGLFFSDQVEYVTSTFAYSIVQAFNELEEAWPEICHDIT 260
           CSP EVI   D  Q+ YCHLL G+ F DQV+YV + FA+ +V AF   E+ W EI  DI 
Sbjct: 193 CSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDIK 252

Query: 261 HGTLSSRITLPKIRKAVLNVIRPNPGLAAEIRGQCEELEGEDWLGLIPKLWRNCKYVYSI 320
            G LS+RIT+P +R A+  ++ PNP LA  IR +C  L   +W GLIP L+ N KYVY I
Sbjct: 253 DGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIPALFPNAKYVYGI 312

Query: 321 MTGSMQPYLKKLRHYAGGLALVSADYGSTESWIGVNVEPGLPPENVTFAVIPTFAYFEFI 380
           MTGSM+PY+ KLRHYAG L LVS DYGS+E WI  NV P L PE  TFAVIP   YFEF+
Sbjct: 313 MTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEFL 372

Query: 381 PLFRQHQQQQHYCCGSSDIATDDFLEGQPVPLSQVKIGQEYELVLTTFTGLYRCRLGDVV 440
           P+    + ++                 +PV L+QVKIG+EYE+V+T + GLYR RLGDVV
Sbjct: 373 PVSETGEGEE-----------------KPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVV 432

Query: 441 EVAGFYNKTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSHA 500
           +V GFYN TP+L FICRR L+L+INIDKNTE+DLQL VE  ++ L+++   E++DF+S+ 
Sbjct: 433 KVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK-IEVIDFSSYI 492

Query: 501 DLADQPGHYVIFWEVKGHVDDTLLGKCCSEMDAAFVDYGYVVSRRSNSIGPLELRIVERG 560
           D++  PGHY IFWE+ G  ++ +L  CC+ +D AF+D GYV SR+  +IG LELR+V +G
Sbjct: 493 DVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKG 552

Query: 561 TFNKILEHYIGNGAALSQFKTPRCTR--NQVLLNILNLSTIKTFRSTAY 608
           TF KI EH++G G++  QFK PRC +  N  +L IL  + + ++ STA+
Sbjct: 553 TFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

[Close]

BLAST of Lcy07g012510 vs. ExPASy TrEMBL
Match: A0A6J1J741 (indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita maxima OX=3661 GN=LOC111484069 PE=3 SV=1)

HSP 1 Score: 1078.5 bits (2788), Expect = 0.0e+00
Identity = 520/589 (88.29%), Postives = 556/589 (94.40%), Query Frame = 0


Query: 19  EEMIDWFEQVSSEAGVAQSRTLREILRENCGVEYLKQWLGDVNIHEITDDFALESLFTSL 78
           E+M+DWFE+VSSEAGVAQSRTLREILR+NCGVEYL QWLGDVNIH+I DDFALESLFTSL
Sbjct: 6   EDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 65

Query: 79  VPLSSHADLEPYLQRIADGDSAPLLTQQPINTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 138
           VPLSSHAD EP+LQRIADGDS+PLLTQQPI TLSLSSGTTEGRQKYVPFTPHSAQTTLLI
Sbjct: 66  VPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 125

Query: 139 FRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLETKS 198
           FR+AAAYRSRVYPTREGG+ILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQL+TKS
Sbjct: 126 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 185

Query: 199 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEYVTSTFAYSIVQAFNELEEAWPEICHD 258
           FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVE+VTSTFAY+IVQAFNELE+ WP+ICHD
Sbjct: 186 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 245

Query: 259 ITHGTLSSRITLPKIRKAVLNVIRPNPGLAAEIRGQCEELEGEDWLGLIPKLWRNCKYVY 318
           ITH TL SRITLP+IRKAVLN+I PNP L A+I+  CEELE +DWL L+PKLW NCKYVY
Sbjct: 246 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSNCKYVY 305

Query: 319 SIMTGSMQPYLKKLRHYAGGLALVSADYGSTESWIGVNVEPGLPPENVTFAVIPTFAYFE 378
           SIMTGSMQPYLKKLR YAGGL LVS DYGSTESWIG+NV+P LPPENVTFAV+PTF+YFE
Sbjct: 306 SIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPTFSYFE 365

Query: 379 FIPLFRQHQQQQHYCCGSSDIATDDFLEGQPVPLSQVKIGQEYELVLTTFTGLYRCRLGD 438
           FIPLFRQH  QQH C  SS  A DDFLEGQP+PLSQVKIGQ+YELVLTTFTGLYRCRLGD
Sbjct: 366 FIPLFRQH--QQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGD 425

Query: 439 VVEVAGFYNKTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS 498
           VVEVAGF+NKTPKLNFICRRKLVLT+NIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS
Sbjct: 426 VVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS 485

Query: 499 HADLADQPGHYVIFWEVKGHVDDTLLGKCCSEMDAAFVDYGYVVSRRSNSIGPLELRIVE 558
           HADL +QPGHYVIFWEVKG+VDD +LG+CCSEMDAAFVDYGYVVSRRSN+IGPLELRIVE
Sbjct: 486 HADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVE 545

Query: 559 RGTFNKILEHYIGNGAALSQFKTPRCTRNQVLLNILNLSTIKTFRSTAY 608
           RG+FNKI+EHYIGNGAALSQFKTPRCT NQVLL ILNLST+K F STAY
Sbjct: 546 RGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 592

[Close]

BLAST of Lcy07g012510 vs. ExPASy TrEMBL
Match: A0A6J1FRQ1 (indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita moschata OX=3662 GN=LOC111448207 PE=3 SV=1)

HSP 1 Score: 1071.6 bits (2770), Expect = 1.1e-309
Identity = 518/589 (87.95%), Postives = 552/589 (93.72%), Query Frame = 0


Query: 19  EEMIDWFEQVSSEAGVAQSRTLREILRENCGVEYLKQWLGDVNIHEITDDFALESLFTSL 78
           E+M+DWFE+VSSEAGVAQ RTLREILR+NCGVEYL QWLGDVNIH+I DDFALESLFTSL
Sbjct: 6   EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 65

Query: 79  VPLSSHADLEPYLQRIADGDSAPLLTQQPINTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 138
           +PLSSHAD EP+LQRIADGDS+PLLTQQPI TLSLSSGTTEGRQKYVPFTPHSAQTTLLI
Sbjct: 66  IPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 125

Query: 139 FRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLETKS 198
           FR+AAAYRSRVYPTREGG+ILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQL+TKS
Sbjct: 126 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 185

Query: 199 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEYVTSTFAYSIVQAFNELEEAWPEICHD 258
           FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVE+VTSTFAY+IVQAFNELE+ WP+ICHD
Sbjct: 186 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 245

Query: 259 ITHGTLSSRITLPKIRKAVLNVIRPNPGLAAEIRGQCEELEGEDWLGLIPKLWRNCKYVY 318
           ITH TL SRITLP+IRKAVLN+I PNP L A+I   CEELE +DWL L+PKLW NCKYVY
Sbjct: 246 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCKYVY 305

Query: 319 SIMTGSMQPYLKKLRHYAGGLALVSADYGSTESWIGVNVEPGLPPENVTFAVIPTFAYFE 378
           SIMTGSMQPYLKKLR YAG L LVS DYGSTESWIGVNV+P LPPENVTFAV+PTF+YFE
Sbjct: 306 SIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYFE 365

Query: 379 FIPLFRQHQQQQHYCCGSSDIATDDFLEGQPVPLSQVKIGQEYELVLTTFTGLYRCRLGD 438
           FIPLFRQH  QQH C  SS  A DDFLEGQP+PLSQVKIGQ+YELVLTTFTGLYRCRLGD
Sbjct: 366 FIPLFRQH--QQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGD 425

Query: 439 VVEVAGFYNKTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS 498
           VVEVAGF+NKTPKLNFICRRKLVLT+NIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS
Sbjct: 426 VVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS 485

Query: 499 HADLADQPGHYVIFWEVKGHVDDTLLGKCCSEMDAAFVDYGYVVSRRSNSIGPLELRIVE 558
           HADL +QPGHYVIFWEVKGHV+D +LG CCSEMDAAFVDYGYVVSRRSN+IGPLELRIVE
Sbjct: 486 HADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVE 545

Query: 559 RGTFNKILEHYIGNGAALSQFKTPRCTRNQVLLNILNLSTIKTFRSTAY 608
           RG+FNKI+EHYIGNGAALSQFKTPRCT NQVLL ILNLST+K F STAY
Sbjct: 546 RGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 592

[Close]

BLAST of Lcy07g012510 vs. ExPASy TrEMBL
Match: A0A6J1CHT7 (indole-3-acetic acid-amido synthetase GH3.10 OS=Momordica charantia OX=3673 GN=LOC111011043 PE=3 SV=1)

HSP 1 Score: 1012.7 bits (2617), Expect = 6.5e-292
Identity = 488/613 (79.61%), Postives = 547/613 (89.23%), Query Frame = 0


Query: 4   DEKGEVGGGG-----VRNNDEEMIDWFEQVSSEAGVAQSRTLREILRENCGVEYLKQWLG 63
           + + E GG G     +   ++E+IDWFEQV+S+AGVAQSRTLR+IL++NCGVEYLK WLG
Sbjct: 2   EAEAEAGGAGNDHGELSEKEQEIIDWFEQVTSDAGVAQSRTLRQILQQNCGVEYLKPWLG 61

Query: 64  DVNIHEI-TDDFALESLFTSLVPLSSHADLEPYLQRIADGDSAPLLTQQPINTLSLSSGT 123
           DVNIH+I  DD ALESLFTS VPLSSH   EP+LQRIADGDS PLLTQ PI TLSLSSGT
Sbjct: 62  DVNIHQIQDDDSALESLFTSSVPLSSHPHFEPFLQRIADGDSTPLLTQLPIKTLSLSSGT 121

Query: 124 TEGRQKYVPFTPHSAQTTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTG 183
           TEGRQKYV FTPHSAQTTLLIFRLAAAYRSRVYP REGGKI+EFIYSSKQTKTKGGITTG
Sbjct: 122 TEGRQKYVLFTPHSAQTTLLIFRLAAAYRSRVYPIREGGKIVEFIYSSKQTKTKGGITTG 181

Query: 184 TATTHYYASEQFKIKQLETKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEYVTSTF 243
           TATTHYYASE+FKIKQL+T+SFTCSP+EVISGGDYKQSTYCHLLLGLFF+ QVE+VTSTF
Sbjct: 182 TATTHYYASEEFKIKQLKTQSFTCSPEEVISGGDYKQSTYCHLLLGLFFAHQVEFVTSTF 241

Query: 244 AYSIVQAFNELEEAWPEICHDITHGTLSSRITLPKIRKAVLNVIRPNPGLAAEIRGQCEE 303
           AY+I+QAFN+LEE WP+IC DITH +LSS +T+PK+R+AVL++I PNP LA+ IR  C+ 
Sbjct: 242 AYTILQAFNQLEEVWPDICRDITHASLSSTVTIPKMRRAVLDIISPNPSLASSIRASCQR 301

Query: 304 LEGEDWLGLIPKLWRNCKYVYSIMTGSMQPYLKKLRHYAGGLALVSADYGSTESWIGVNV 363
           L   DW GLIP+LW N KYVYSI TGSMQPY+KKLRHYA GL LVSADYGSTESWIGVNV
Sbjct: 302 LRARDWFGLIPELWPNAKYVYSITTGSMQPYVKKLRHYAAGLPLVSADYGSTESWIGVNV 361

Query: 364 EPGLPPENVTFAVIPTFAYFEFIPLFRQHQQQQHYCCGSSDIATDDFLEGQPVPLSQVKI 423
           +P LPPENVTFAV+PTF+YFEFIPLFRQHQ Q   C    DI  DDFLEG+PVPLS+VKI
Sbjct: 362 DPSLPPENVTFAVVPTFSYFEFIPLFRQHQNQNQICSSDFDIGVDDFLEGEPVPLSRVKI 421

Query: 424 GQEYELVLTTFTGLYRCRLGDVVEVAGFYNKTPKLNFICRRKLVLTINIDKNTEKDLQLV 483
           GQ+YE+VLTTFTGLYRCRLGD+VEV+GF+NKTPKL+FICRRKLVLTINIDKNTEKDLQ+V
Sbjct: 422 GQQYEVVLTTFTGLYRCRLGDIVEVSGFHNKTPKLSFICRRKLVLTINIDKNTEKDLQMV 481

Query: 484 VERGSQLLAD--QSGAELVDFTSHADLADQPGHYVIFWEVKGHVDDTLLGKCCSEMDAAF 543
           VERG+QLL D  QSGAE+VDFTSHAD+ADQPGHYVIFWEVKG V+D +LG+CC+ MDAAF
Sbjct: 482 VERGAQLLGDKSQSGAEVVDFTSHADVADQPGHYVIFWEVKGDVEDAVLGECCTLMDAAF 541

Query: 544 VDYGYVVSRRSNSIGPLELRIVERGTFNKILEHYIGNGAALSQFKTPRCTRNQVLLNILN 603
           VD+GYVVSRRSNSIGPLELR+VERGTF KILEH IGNG ALSQFKTPRCT NQ+LL+ILN
Sbjct: 542 VDHGYVVSRRSNSIGPLELRVVERGTFKKILEHCIGNGGALSQFKTPRCTTNQLLLSILN 601

Query: 604 LSTIKTFRSTAYA 609
           LST+KTFRSTAYA
Sbjct: 602 LSTVKTFRSTAYA 614

[Close]

BLAST of Lcy07g012510 vs. ExPASy TrEMBL
Match: A0A5D3B9T4 (Jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold464G00300 PE=3 SV=1)

HSP 1 Score: 1007.3 bits (2603), Expect = 2.7e-290
Identity = 488/591 (82.57%), Postives = 541/591 (91.54%), Query Frame = 0


Query: 18  DEEMIDWFEQVSSEAGVAQSRTLREILRENCGVEYLKQWLGDVNIHEITDDFALESLFTS 77
           +E++IDWFEQVSSEA VAQSRTLR+IL++N GVEYLK+W+GDVNIHEI DDF L+S+FTS
Sbjct: 10  EEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDDFTLQSIFTS 69

Query: 78  LVPLSSHADLEPYLQRIADGDSAPLLTQQPINTLSLSSGTTEGRQKYVPFTPHSAQTTLL 137
           L+PLSSHA  EP+LQRIADGDS+PLLTQQPI TLSLSSGTTEGRQKYVPFTPHSA TTLL
Sbjct: 70  LIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTPHSAHTTLL 129

Query: 138 IFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLETK 197
           IFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASE+FKIKQL+TK
Sbjct: 130 IFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLKTK 189

Query: 198 SFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEYVTSTFAYSIVQAFNELEEAWPEICH 257
           SFTCSPQEVISG DYKQSTYCHLLLGL +S++VE+VTSTFAY+IVQAFN+LEE+W EI H
Sbjct: 190 SFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQAFNQLEESWEEIIH 249

Query: 258 DITHGTLSSRITLPKIRKAVLNVIRPNPGLAAEIRGQCEELEGEDWLGLIPKLWRNCKYV 317
           DI+H TLSSRI +P+IRKAVL V+ P P L  +IR  CEEL+ E WLGLIPKLW NCKYV
Sbjct: 250 DISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKKEGWLGLIPKLWPNCKYV 309

Query: 318 YSIMTGSMQPYLKKLRHYAGGLALVSADYGSTESWIGVNVEPGLPPENVTFAVIPTFAYF 377
           YSIMTGSMQPYLKKLRHY GGL LVS DYGSTESWIGVNV+P LPPENVTFAVIPTF+YF
Sbjct: 310 YSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPTFSYF 369

Query: 378 EFIPLFRQHQQQQHYCCGSSDIATDDFLEGQPVPLSQVKIGQEYELVLTTFTGLYRCRLG 437
           EFIPLF+QH         S+D A  DFLEGQP+ LS+VKIGQ+YELVLTTFTGLYRCRLG
Sbjct: 370 EFIPLFKQHA-----TTSSTDTAIHDFLEGQPIALSEVKIGQQYELVLTTFTGLYRCRLG 429

Query: 438 DVVEVAGFYNKTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGSQLLADQSGAELVDFT 497
           DVVEVAGF+NKTPKLNFICRRKLVLT+NIDKNTEKD+QL VERGSQL++  SGAELVDFT
Sbjct: 430 DVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQLMSQLSGAELVDFT 489

Query: 498 SHADLADQPGHYVIFWEVKGHVDDTLLGKCCSEMDAAFVDYGYVVSRRSNSIGPLELRIV 557
           S+A+L++QPGHYVIFWEVKG V+DT+LGKCCSEMDAAFVDYGYVVSR++NSIGPLELRIV
Sbjct: 490 SYAELSNQPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVVSRKANSIGPLELRIV 549

Query: 558 ERGTFNKILEHYIGNGAALSQFKTPRCTRNQVLLNILNLSTIKTFRSTAYA 609
           ERG+FNKILE+YIGNGAALSQFKTPRCT N  LLNILNLST+++F STAYA
Sbjct: 550 ERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSFFSTAYA 595

[Close]

BLAST of Lcy07g012510 vs. ExPASy TrEMBL
Match: A0A1S3BIL6 (jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490483 PE=3 SV=1)

HSP 1 Score: 1007.3 bits (2603), Expect = 2.7e-290
Identity = 488/591 (82.57%), Postives = 541/591 (91.54%), Query Frame = 0


Query: 18  DEEMIDWFEQVSSEAGVAQSRTLREILRENCGVEYLKQWLGDVNIHEITDDFALESLFTS 77
           +E++IDWFEQVSSEA VAQSRTLR+IL++N GVEYLK+W+GDVNIHEI DDF L+S+FTS
Sbjct: 10  EEDIIDWFEQVSSEAAVAQSRTLRKILQQNYGVEYLKKWVGDVNIHEIPDDFTLQSIFTS 69

Query: 78  LVPLSSHADLEPYLQRIADGDSAPLLTQQPINTLSLSSGTTEGRQKYVPFTPHSAQTTLL 137
           L+PLSSHA  EP+LQRIADGDS+PLLTQQPI TLSLSSGTTEGRQKYVPFTPHSA TTLL
Sbjct: 70  LIPLSSHAHFEPFLQRIADGDSSPLLTQQPITTLSLSSGTTEGRQKYVPFTPHSAHTTLL 129

Query: 138 IFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLETK 197
           IFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASE+FKIKQL+TK
Sbjct: 130 IFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEEFKIKQLKTK 189

Query: 198 SFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEYVTSTFAYSIVQAFNELEEAWPEICH 257
           SFTCSPQEVISG DYKQSTYCHLLLGL +S++VE+VTSTFAY+IVQAFN+LEE+W EI H
Sbjct: 190 SFTCSPQEVISGCDYKQSTYCHLLLGLLYSEEVEFVTSTFAYTIVQAFNQLEESWEEIIH 249

Query: 258 DITHGTLSSRITLPKIRKAVLNVIRPNPGLAAEIRGQCEELEGEDWLGLIPKLWRNCKYV 317
           DI+H TLSSRI +P+IRKAVL V+ P P L  +IR  CEEL+ E WLGLIPKLW NCKYV
Sbjct: 250 DISHATLSSRIDIPEIRKAVLKVMSPKPELGWKIRRVCEELKKEGWLGLIPKLWPNCKYV 309

Query: 318 YSIMTGSMQPYLKKLRHYAGGLALVSADYGSTESWIGVNVEPGLPPENVTFAVIPTFAYF 377
           YSIMTGSMQPYLKKLRHY GGL LVS DYGSTESWIGVNV+P LPPENVTFAVIPTF+YF
Sbjct: 310 YSIMTGSMQPYLKKLRHYGGGLPLVSGDYGSTESWIGVNVDPYLPPENVTFAVIPTFSYF 369

Query: 378 EFIPLFRQHQQQQHYCCGSSDIATDDFLEGQPVPLSQVKIGQEYELVLTTFTGLYRCRLG 437
           EFIPLF+QH         S+D A  DFLEGQP+ LS+VKIGQ+YELVLTTFTGLYRCRLG
Sbjct: 370 EFIPLFKQHA-----TTSSTDTAIHDFLEGQPIALSEVKIGQQYELVLTTFTGLYRCRLG 429

Query: 438 DVVEVAGFYNKTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGSQLLADQSGAELVDFT 497
           DVVEVAGF+NKTPKLNFICRRKLVLT+NIDKNTEKD+QL VERGSQL++  SGAELVDFT
Sbjct: 430 DVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDVQLAVERGSQLMSQLSGAELVDFT 489

Query: 498 SHADLADQPGHYVIFWEVKGHVDDTLLGKCCSEMDAAFVDYGYVVSRRSNSIGPLELRIV 557
           S+A+L++QPGHYVIFWEVKG V+DT+LGKCCSEMDAAFVDYGYVVSR++NSIGPLELRIV
Sbjct: 490 SYAELSNQPGHYVIFWEVKGDVNDTVLGKCCSEMDAAFVDYGYVVSRKANSIGPLELRIV 549

Query: 558 ERGTFNKILEHYIGNGAALSQFKTPRCTRNQVLLNILNLSTIKTFRSTAYA 609
           ERG+FNKILE+YIGNGAALSQFKTPRCT N  LLNILNLST+++F STAYA
Sbjct: 550 ERGSFNKILEYYIGNGAALSQFKTPRCTTNHFLLNILNLSTLQSFFSTAYA 595

[Close]

BLAST of Lcy07g012510 vs. NCBI nr
Match: XP_022986272.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita maxima])

HSP 1 Score: 1078.5 bits (2788), Expect = 0.0e+00
Identity = 520/589 (88.29%), Postives = 556/589 (94.40%), Query Frame = 0


Query: 19  EEMIDWFEQVSSEAGVAQSRTLREILRENCGVEYLKQWLGDVNIHEITDDFALESLFTSL 78
           E+M+DWFE+VSSEAGVAQSRTLREILR+NCGVEYL QWLGDVNIH+I DDFALESLFTSL
Sbjct: 6   EDMVDWFERVSSEAGVAQSRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 65

Query: 79  VPLSSHADLEPYLQRIADGDSAPLLTQQPINTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 138
           VPLSSHAD EP+LQRIADGDS+PLLTQQPI TLSLSSGTTEGRQKYVPFTPHSAQTTLLI
Sbjct: 66  VPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 125

Query: 139 FRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLETKS 198
           FR+AAAYRSRVYPTREGG+ILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQL+TKS
Sbjct: 126 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 185

Query: 199 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEYVTSTFAYSIVQAFNELEEAWPEICHD 258
           FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVE+VTSTFAY+IVQAFNELE+ WP+ICHD
Sbjct: 186 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 245

Query: 259 ITHGTLSSRITLPKIRKAVLNVIRPNPGLAAEIRGQCEELEGEDWLGLIPKLWRNCKYVY 318
           ITH TL SRITLP+IRKAVLN+I PNP L A+I+  CEELE +DWL L+PKLW NCKYVY
Sbjct: 246 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDWLDLVPKLWSNCKYVY 305

Query: 319 SIMTGSMQPYLKKLRHYAGGLALVSADYGSTESWIGVNVEPGLPPENVTFAVIPTFAYFE 378
           SIMTGSMQPYLKKLR YAGGL LVS DYGSTESWIG+NV+P LPPENVTFAV+PTF+YFE
Sbjct: 306 SIMTGSMQPYLKKLRRYAGGLPLVSGDYGSTESWIGINVDPCLPPENVTFAVVPTFSYFE 365

Query: 379 FIPLFRQHQQQQHYCCGSSDIATDDFLEGQPVPLSQVKIGQEYELVLTTFTGLYRCRLGD 438
           FIPLFRQH  QQH C  SS  A DDFLEGQP+PLSQVKIGQ+YELVLTTFTGLYRCRLGD
Sbjct: 366 FIPLFRQH--QQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGD 425

Query: 439 VVEVAGFYNKTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS 498
           VVEVAGF+NKTPKLNFICRRKLVLT+NIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS
Sbjct: 426 VVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS 485

Query: 499 HADLADQPGHYVIFWEVKGHVDDTLLGKCCSEMDAAFVDYGYVVSRRSNSIGPLELRIVE 558
           HADL +QPGHYVIFWEVKG+VDD +LG+CCSEMDAAFVDYGYVVSRRSN+IGPLELRIVE
Sbjct: 486 HADLTNQPGHYVIFWEVKGNVDDAVLGRCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVE 545

Query: 559 RGTFNKILEHYIGNGAALSQFKTPRCTRNQVLLNILNLSTIKTFRSTAY 608
           RG+FNKI+EHYIGNGAALSQFKTPRCT NQVLL ILNLST+K F STAY
Sbjct: 546 RGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 592

[Close]

BLAST of Lcy07g012510 vs. NCBI nr
Match: KAG6570848.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1071.6 bits (2770), Expect = 2.3e-309
Identity = 518/589 (87.95%), Postives = 552/589 (93.72%), Query Frame = 0


Query: 19  EEMIDWFEQVSSEAGVAQSRTLREILRENCGVEYLKQWLGDVNIHEITDDFALESLFTSL 78
           E+M+DWFE+VSSEAGVAQ RTLREILR+NCGVEYL QWLGDVNIH+I DDFALESLFTSL
Sbjct: 6   EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 65

Query: 79  VPLSSHADLEPYLQRIADGDSAPLLTQQPINTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 138
           +PLSSHAD EP+LQRIADGDS+PLLTQQPI TLSLSSGTTEGRQKYVPFTPHSAQTTLLI
Sbjct: 66  IPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 125

Query: 139 FRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLETKS 198
           FR+AAAYRSRVYPTREGG+ILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQL+TKS
Sbjct: 126 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 185

Query: 199 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEYVTSTFAYSIVQAFNELEEAWPEICHD 258
           FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVE+VTSTFAY+IVQAFNELE+ WP+ICHD
Sbjct: 186 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 245

Query: 259 ITHGTLSSRITLPKIRKAVLNVIRPNPGLAAEIRGQCEELEGEDWLGLIPKLWRNCKYVY 318
           ITH TL SRITLP+IRKAVLN+I PNP L A+I   CEELE +DWL L+PKLW NCKYVY
Sbjct: 246 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCKYVY 305

Query: 319 SIMTGSMQPYLKKLRHYAGGLALVSADYGSTESWIGVNVEPGLPPENVTFAVIPTFAYFE 378
           SIMTGSMQPYLKKLR YAG L LVS DYGSTESWIGVNV+P LPPENVTFAV+PTF+YFE
Sbjct: 306 SIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYFE 365

Query: 379 FIPLFRQHQQQQHYCCGSSDIATDDFLEGQPVPLSQVKIGQEYELVLTTFTGLYRCRLGD 438
           FIPLFRQH  QQH C  SS  A DDFLEGQP+PLSQVKIGQ+YELVLTTFTGLYRCRLGD
Sbjct: 366 FIPLFRQH--QQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGD 425

Query: 439 VVEVAGFYNKTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS 498
           VVEVAGF+NKTPKLNFICRRKLVLT+NIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS
Sbjct: 426 VVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS 485

Query: 499 HADLADQPGHYVIFWEVKGHVDDTLLGKCCSEMDAAFVDYGYVVSRRSNSIGPLELRIVE 558
           HADL +QPGHYVIFWEVKGHV+D +LG CCSEMDAAFVDYGYVVSRRSN+IGPLELRIVE
Sbjct: 486 HADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVE 545

Query: 559 RGTFNKILEHYIGNGAALSQFKTPRCTRNQVLLNILNLSTIKTFRSTAY 608
           RG+FNKI+EHYIGNGAALSQFKTPRCT NQVLL ILNLST+K F STAY
Sbjct: 546 RGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 592

[Close]

BLAST of Lcy07g012510 vs. NCBI nr
Match: XP_022943441.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita moschata])

HSP 1 Score: 1071.6 bits (2770), Expect = 2.3e-309
Identity = 518/589 (87.95%), Postives = 552/589 (93.72%), Query Frame = 0


Query: 19  EEMIDWFEQVSSEAGVAQSRTLREILRENCGVEYLKQWLGDVNIHEITDDFALESLFTSL 78
           E+M+DWFE+VSSEAGVAQ RTLREILR+NCGVEYL QWLGDVNIH+I DDFALESLFTSL
Sbjct: 6   EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 65

Query: 79  VPLSSHADLEPYLQRIADGDSAPLLTQQPINTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 138
           +PLSSHAD EP+LQRIADGDS+PLLTQQPI TLSLSSGTTEGRQKYVPFTPHSAQTTLLI
Sbjct: 66  IPLSSHADFEPFLQRIADGDSSPLLTQQPIRTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 125

Query: 139 FRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLETKS 198
           FR+AAAYRSRVYPTREGG+ILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQL+TKS
Sbjct: 126 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 185

Query: 199 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEYVTSTFAYSIVQAFNELEEAWPEICHD 258
           FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVE+VTSTFAY+IVQAFNELE+ WP+ICHD
Sbjct: 186 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 245

Query: 259 ITHGTLSSRITLPKIRKAVLNVIRPNPGLAAEIRGQCEELEGEDWLGLIPKLWRNCKYVY 318
           ITH TL SRITLP+IRKAVLN+I PNP L A+I   CEELE +DWL L+PKLW NCKYVY
Sbjct: 246 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCKYVY 305

Query: 319 SIMTGSMQPYLKKLRHYAGGLALVSADYGSTESWIGVNVEPGLPPENVTFAVIPTFAYFE 378
           SIMTGSMQPYLKKLR YAG L LVS DYGSTESWIGVNV+P LPPENVTFAV+PTF+YFE
Sbjct: 306 SIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYFE 365

Query: 379 FIPLFRQHQQQQHYCCGSSDIATDDFLEGQPVPLSQVKIGQEYELVLTTFTGLYRCRLGD 438
           FIPLFRQH  QQH C  SS  A DDFLEGQP+PLSQVKIGQ+YELVLTTFTGLYRCRLGD
Sbjct: 366 FIPLFRQH--QQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGD 425

Query: 439 VVEVAGFYNKTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS 498
           VVEVAGF+NKTPKLNFICRRKLVLT+NIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS
Sbjct: 426 VVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS 485

Query: 499 HADLADQPGHYVIFWEVKGHVDDTLLGKCCSEMDAAFVDYGYVVSRRSNSIGPLELRIVE 558
           HADL +QPGHYVIFWEVKGHV+D +LG CCSEMDAAFVDYGYVVSRRSN+IGPLELRIVE
Sbjct: 486 HADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVE 545

Query: 559 RGTFNKILEHYIGNGAALSQFKTPRCTRNQVLLNILNLSTIKTFRSTAY 608
           RG+FNKI+EHYIGNGAALSQFKTPRCT NQVLL ILNLST+K F STAY
Sbjct: 546 RGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 592

[Close]

BLAST of Lcy07g012510 vs. NCBI nr
Match: XP_023512179.1 (indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1070.5 bits (2767), Expect = 5.6e-309
Identity = 518/589 (87.95%), Postives = 552/589 (93.72%), Query Frame = 0


Query: 19  EEMIDWFEQVSSEAGVAQSRTLREILRENCGVEYLKQWLGDVNIHEITDDFALESLFTSL 78
           E+M+DWFE+VSSEAGVAQ RTLREILR+NCGVEYL QWLGDVNIH+I DDFALESLFTSL
Sbjct: 6   EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 65

Query: 79  VPLSSHADLEPYLQRIADGDSAPLLTQQPINTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 138
           VPLSSHAD EP+LQRIADGDS+PLLTQQPI TLSLSSGTTEGRQKYVPFTPHSAQTTLLI
Sbjct: 66  VPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 125

Query: 139 FRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLETKS 198
           FR+AAAYRSRVYPTREGG+ILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQL+TKS
Sbjct: 126 FRIAAAYRSRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLKTKS 185

Query: 199 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEYVTSTFAYSIVQAFNELEEAWPEICHD 258
           FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVE+VTSTFAY+IVQAFNELE+ WP+ICHD
Sbjct: 186 FTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQAFNELEDCWPQICHD 245

Query: 259 ITHGTLSSRITLPKIRKAVLNVIRPNPGLAAEIRGQCEELEGEDWLGLIPKLWRNCKYVY 318
           ITH TL SRITLP+IRKAVLN+I PNP L A+I   CEELE +DWL L+PKLW NCKYVY
Sbjct: 246 ITHATLCSRITLPEIRKAVLNIISPNPILGAKIGAVCEELERQDWLDLVPKLWPNCKYVY 305

Query: 319 SIMTGSMQPYLKKLRHYAGGLALVSADYGSTESWIGVNVEPGLPPENVTFAVIPTFAYFE 378
           SIMTGS+QPYLKKLR YAG L LVS DYGSTESWIGVNV+P LPPENVTFAV+PTF+YFE
Sbjct: 306 SIMTGSIQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPPENVTFAVVPTFSYFE 365

Query: 379 FIPLFRQHQQQQHYCCGSSDIATDDFLEGQPVPLSQVKIGQEYELVLTTFTGLYRCRLGD 438
           FIPLFRQH  QQH C  SS  A DDFLEGQP+PLSQVKIGQ+YELVLTTFTGLYRCRLGD
Sbjct: 366 FIPLFRQH--QQHDCGSSSVAAFDDFLEGQPLPLSQVKIGQQYELVLTTFTGLYRCRLGD 425

Query: 439 VVEVAGFYNKTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS 498
           VVEVAGF+NKTPKLNFICRRKLVLT+NIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS
Sbjct: 426 VVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGSQLLADQSGAELVDFTS 485

Query: 499 HADLADQPGHYVIFWEVKGHVDDTLLGKCCSEMDAAFVDYGYVVSRRSNSIGPLELRIVE 558
           HADL +QPGHYVIFWEVKGHV+D +LG CCSEMDAAFVDYGYVVSRRSN+IGPLELRIVE
Sbjct: 486 HADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVVSRRSNTIGPLELRIVE 545

Query: 559 RGTFNKILEHYIGNGAALSQFKTPRCTRNQVLLNILNLSTIKTFRSTAY 608
           RG+FNKI+EHYIGNGAALSQFKTPRCT NQVLL ILNLST+K F STAY
Sbjct: 546 RGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAFHSTAY 592

[Close]

BLAST of Lcy07g012510 vs. NCBI nr
Match: KAG7010696.1 (Indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1063.1 bits (2748), Expect = 8.7e-307
Identity = 518/605 (85.62%), Postives = 554/605 (91.57%), Query Frame = 0


Query: 19  EEMIDWFEQVSSEAGVAQSRTLREILRENCGVEYLKQWLGDVNIHEITDDFALESLFTSL 78
           E+M+DWFE+VSSEAGVAQ RTLREILR+NCGVEYL QWLGDVNIH+I DDFALESLFTSL
Sbjct: 6   EDMVDWFERVSSEAGVAQLRTLREILRQNCGVEYLNQWLGDVNIHQIPDDFALESLFTSL 65

Query: 79  VPLSSHADLEPYLQRIADGDSAPLLTQQPINTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 138
           +PLSSHAD EP+LQRIADGDS+PLLTQQPI TLSLSSGTTEGRQKYVPFTPHSAQTTLLI
Sbjct: 66  IPLSSHADFEPFLQRIADGDSSPLLTQQPIQTLSLSSGTTEGRQKYVPFTPHSAQTTLLI 125

Query: 139 FRLAAAYRS---------------RVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHY 198
           FR+AAAYRS               RVYPTREGG+ILEFIYSSKQTKTKGGITTGTATTHY
Sbjct: 126 FRIAAAYRSRYQCNMTLKNGLITTRVYPTREGGRILEFIYSSKQTKTKGGITTGTATTHY 185

Query: 199 YASEQFKIKQLETKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEYVTSTFAYSIVQ 258
           YASEQFKIKQL+TKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVE+VTSTFAY+IVQ
Sbjct: 186 YASEQFKIKQLKTKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEFVTSTFAYTIVQ 245

Query: 259 AFNELEEAWPEICHDITHGTLSSRITLPKIRKAVLNVIRPNPGLAAEIRGQCEELEGEDW 318
           AFNELE+ WP+ICHDITH TL SRITLP+IRKAVLN+I PNP L A+I+  CEELE +DW
Sbjct: 246 AFNELEDCWPQICHDITHATLCSRITLPEIRKAVLNIISPNPILGAKIQAVCEELERQDW 305

Query: 319 LGLIPKLWRNCKYVYSIMTGSMQPYLKKLRHYAGGLALVSADYGSTESWIGVNVEPGLPP 378
           L L+PKLW NCKYVYSIMTGSMQPYLKKLR YAG L LVS DYGSTESWIGVNV+P LPP
Sbjct: 306 LDLVPKLWPNCKYVYSIMTGSMQPYLKKLRRYAGRLPLVSGDYGSTESWIGVNVDPCLPP 365

Query: 379 ENVTFAVIPTFAYFEFIPLFRQHQQQQHYCCGSSDIAT-DDFLEGQPVPLSQVKIGQEYE 438
           ENVTFAV+PTF+YFEFIPLFRQHQQ     CGSS +A  DDFLEGQP+PLSQVKIGQ+YE
Sbjct: 366 ENVTFAVVPTFSYFEFIPLFRQHQQHD---CGSSSVAVFDDFLEGQPLPLSQVKIGQQYE 425

Query: 439 LVLTTFTGLYRCRLGDVVEVAGFYNKTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGS 498
           LVLTTFTGLYRCRLGDVVEVAGF+NKTPKLNFICRRKLVLT+NIDKNTEKDLQLVVERGS
Sbjct: 426 LVLTTFTGLYRCRLGDVVEVAGFHNKTPKLNFICRRKLVLTVNIDKNTEKDLQLVVERGS 485

Query: 499 QLLADQSGAELVDFTSHADLADQPGHYVIFWEVKGHVDDTLLGKCCSEMDAAFVDYGYVV 558
           QLLADQSGAELVDFTSHADL +QPGHYVIFWEVKGHV+D +LG CCSEMDAAFVDYGYVV
Sbjct: 486 QLLADQSGAELVDFTSHADLTNQPGHYVIFWEVKGHVEDAVLGHCCSEMDAAFVDYGYVV 545

Query: 559 SRRSNSIGPLELRIVERGTFNKILEHYIGNGAALSQFKTPRCTRNQVLLNILNLSTIKTF 608
           SRRSN+IGPLELRIVERG+FNKI+EHYIGNGAALSQFKTPRCT NQVLL ILNLST+K F
Sbjct: 546 SRRSNTIGPLELRIVERGSFNKIMEHYIGNGAALSQFKTPRCTTNQVLLTILNLSTVKAF 605

[Close]

BLAST of Lcy07g012510 vs. TAIR 10
Match: AT4G03400.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 837.8 bits (2163), Expect = 5.5e-243
Identity = 416/596 (69.80%), Postives = 481/596 (80.70%), Query Frame = 0


Query: 14  VRNNDEEMIDWFEQVSSEAGVAQSRTLREILRENCGVEYLKQWLGDVNIHEITDDFALES 73
           V    +++I WFE VS  A   QS TLR IL  N GVEYL++WLG V++ ++ DD+ LE+
Sbjct: 4   VEAGHDDVIGWFEHVSENACKVQSETLRRILELNSGVEYLRKWLGTVDVEKM-DDYTLET 63

Query: 74  LFTSLVPLSSHADLEPYLQRIADGDSAPLLTQQPINTLSLSSGTTEGRQKYVPFTPHSAQ 133
           LFTSLVP+ SHADL+PY+QRIADG+++PLLTQ+PI  LSLSSGTTEGRQKYVPFT HSAQ
Sbjct: 64  LFTSLVPIVSHADLDPYIQRIADGETSPLLTQEPITVLSLSSGTTEGRQKYVPFTRHSAQ 123

Query: 134 TTLLIFRLAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQ 193
           TTL IFRL+AAYRSR YP REGG+ILEFIY+ K+ KT GG+T GTATTHYYASE+FK KQ
Sbjct: 124 TTLQIFRLSAAYRSRFYPIREGGRILEFIYAGKEFKTLGGLTVGTATTHYYASEEFKTKQ 183

Query: 194 LETKSFTCSPQEVISGGDYKQSTYCHLLLGLFFSDQVEYVTSTFAYSIVQAFNELEEAWP 253
             TKSFTCSPQEVISGGD+ Q TYCHLLLGL +S QVE+V S F+Y+IVQAF+  EE W 
Sbjct: 184 ETTKSFTCSPQEVISGGDFGQCTYCHLLLGLHYSSQVEFVASAFSYTIVQAFSFFEEIWR 243

Query: 254 EICHDITHGTLSSRITLPKIRKAVLNVIRPNPGLAAEIRGQCEELEGE-DWLGLIPKLWR 313
           EIC DI  G LSSRITLPK+RKAVL +IRPNP LA+ I   C ELE    W GLI KLW 
Sbjct: 244 EICADIKEGNLSSRITLPKMRKAVLALIRPNPSLASHIEEICLELETNLGWFGLISKLWP 303

Query: 314 NCKYVYSIMTGSMQPYLKKLRHYAGGLALVSADYGSTESWIGVNVEPGLPPENVTFAVIP 373
           N K++ SIMTGSM PYL KLRHYAGGL LVSADYGSTESWIGVNV+P LPPE+V+FAVIP
Sbjct: 304 NAKFISSIMTGSMLPYLNKLRHYAGGLPLVSADYGSTESWIGVNVDPHLPPEDVSFAVIP 363

Query: 374 TFAYFEFIPLFRQHQQQQHYCCGSSDIATD-DFLEGQPVPLSQVKIGQEYELVLTTFTGL 433
           TF+YFEFIPL+R+  Q        SDI  D DF+E +PVPLSQVK+GQEYELVLTTFTGL
Sbjct: 364 TFSYFEFIPLYRRQNQ--------SDICIDGDFVEDKPVPLSQVKLGQEYELVLTTFTGL 423

Query: 434 YRCRLGDVVEVAGFYNKTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGSQLLADQSGA 493
           YR RLGDVVEV  F+  TPKL+FI RRKL+LTINIDKNTEKDLQ VV++ SQLL+  + A
Sbjct: 424 YRYRLGDVVEVTSFHKGTPKLSFIYRRKLILTINIDKNTEKDLQRVVDKASQLLSRSTRA 483

Query: 494 ELVDFTSHADLADQPGHYVIFWEVKGHVDDTLLGKCCSEMDAAFVDYGYVVSRRSNSIGP 553
           E+VDFTSHAD+  +PGHYVI+WE++G  DD  L +CC EMD AFVDYGYVVSRR NSIGP
Sbjct: 484 EVVDFTSHADVIARPGHYVIYWEIRGEADDKALEECCREMDTAFVDYGYVVSRRMNSIGP 543

Query: 554 LELRIVERGTFNKILEHYIGNGAALSQFKTPRCTRNQVLLNILNLSTIKTFRSTAY 608
           LELR+VERGTF K+ E  +G    L+QFKTPRCT N V+L+ILN STIK FRS+AY
Sbjct: 544 LELRVVERGTFGKVAERCVGKCGGLNQFKTPRCTTNSVMLDILNDSTIKRFRSSAY 590

[Close]

BLAST of Lcy07g012510 vs. TAIR 10
Match: AT2G46370.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 570.5 bits (1469), Expect = 1.6e-162
Identity = 286/589 (48.56%), Postives = 392/589 (66.55%), Query Frame = 0


Query: 21  MIDWFEQVSSEAGVAQSRTLREILRENCGVEYLKQWLGDVNIHEITDDFALESLFTSLVP 80
           +ID F++++  A   Q +TL+EIL +N    YL+    + N    TD    E  F S+VP
Sbjct: 13  VIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGN---ATDP---EEAFKSMVP 72

Query: 81  LSSHADLEPYLQRIADGDSAPLLTQQPINTLSLSSGTTEGRQKYVPFTPHSAQTTLLIFR 140
           L +  +LEPY++R+ DGD++P+LT  P+  +SLSSGT++GR K++PFT    + TL +FR
Sbjct: 73  LVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLFR 132

Query: 141 LAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLETKSFT 200
            A A+R+R +P  + GK L+FI+SSKQ  + GG+  GTATT+ Y +  FK       S +
Sbjct: 133 TAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSPS 192

Query: 201 CSPQEVISGGDYKQSTYCHLLLGLFFSDQVEYVTSTFAYSIVQAFNELEEAWPEICHDIT 260
           CSP EVI   D  Q+ YCHLL G+ F DQV+YV + FA+ +V AF   E+ W EI  DI 
Sbjct: 193 CSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDIK 252

Query: 261 HGTLSSRITLPKIRKAVLNVIRPNPGLAAEIRGQCEELEGEDWLGLIPKLWRNCKYVYSI 320
            G LS+RIT+P +R A+  ++ PNP LA  IR +C  L   +W GLIP L+ N KYVY I
Sbjct: 253 DGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIPALFPNAKYVYGI 312

Query: 321 MTGSMQPYLKKLRHYAGGLALVSADYGSTESWIGVNVEPGLPPENVTFAVIPTFAYFEFI 380
           MTGSM+PY+ KLRHYAG L LVS DYGS+E WI  NV P L PE  TFAVIP   YFEF+
Sbjct: 313 MTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEFL 372

Query: 381 PLFRQHQQQQHYCCGSSDIATDDFLEGQPVPLSQVKIGQEYELVLTTFTGLYRCRLGDVV 440
           P+    + ++                 +PV L+QVKIG+EYE+V+T + GLYR RLGDVV
Sbjct: 373 PVSETGEGEE-----------------KPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVV 432

Query: 441 EVAGFYNKTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSHA 500
           +V GFYN TP+L FICRR L+L+INIDKNTE+DLQL VE  ++ L+++   E++DF+S+ 
Sbjct: 433 KVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK-IEVIDFSSYI 492

Query: 501 DLADQPGHYVIFWEVKGHVDDTLLGKCCSEMDAAFVDYGYVVSRRSNSIGPLELRIVERG 560
           D++  PGHY IFWE+ G  ++ +L  CC+ +D AF+D GYV SR+  +IG LELR+V +G
Sbjct: 493 DVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKG 552

Query: 561 TFNKILEHYIGNGAALSQFKTPRCTR--NQVLLNILNLSTIKTFRSTAY 608
           TF KI EH++G G++  QFK PRC +  N  +L IL  + + ++ STA+
Sbjct: 553 TFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

[Close]

BLAST of Lcy07g012510 vs. TAIR 10
Match: AT2G46370.2 (Auxin-responsive GH3 family protein )

HSP 1 Score: 570.5 bits (1469), Expect = 1.6e-162
Identity = 286/589 (48.56%), Postives = 392/589 (66.55%), Query Frame = 0


Query: 21  MIDWFEQVSSEAGVAQSRTLREILRENCGVEYLKQWLGDVNIHEITDDFALESLFTSLVP 80
           +ID F++++  A   Q +TL+EIL +N    YL+    + N    TD    E  F S+VP
Sbjct: 13  VIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGN---ATDP---EEAFKSMVP 72

Query: 81  LSSHADLEPYLQRIADGDSAPLLTQQPINTLSLSSGTTEGRQKYVPFTPHSAQTTLLIFR 140
           L +  +LEPY++R+ DGD++P+LT  P+  +SLSSGT++GR K++PFT    + TL +FR
Sbjct: 73  LVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLFR 132

Query: 141 LAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLETKSFT 200
            A A+R+R +P  + GK L+FI+SSKQ  + GG+  GTATT+ Y +  FK       S +
Sbjct: 133 TAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSPS 192

Query: 201 CSPQEVISGGDYKQSTYCHLLLGLFFSDQVEYVTSTFAYSIVQAFNELEEAWPEICHDIT 260
           CSP EVI   D  Q+ YCHLL G+ F DQV+YV + FA+ +V AF   E+ W EI  DI 
Sbjct: 193 CSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDIK 252

Query: 261 HGTLSSRITLPKIRKAVLNVIRPNPGLAAEIRGQCEELEGEDWLGLIPKLWRNCKYVYSI 320
            G LS+RIT+P +R A+  ++ PNP LA  IR +C  L   +W GLIP L+ N KYVY I
Sbjct: 253 DGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIPALFPNAKYVYGI 312

Query: 321 MTGSMQPYLKKLRHYAGGLALVSADYGSTESWIGVNVEPGLPPENVTFAVIPTFAYFEFI 380
           MTGSM+PY+ KLRHYAG L LVS DYGS+E WI  NV P L PE  TFAVIP   YFEF+
Sbjct: 313 MTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEFL 372

Query: 381 PLFRQHQQQQHYCCGSSDIATDDFLEGQPVPLSQVKIGQEYELVLTTFTGLYRCRLGDVV 440
           P+    + ++                 +PV L+QVKIG+EYE+V+T + GLYR RLGDVV
Sbjct: 373 PVSETGEGEE-----------------KPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVV 432

Query: 441 EVAGFYNKTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSHA 500
           +V GFYN TP+L FICRR L+L+INIDKNTE+DLQL VE  ++ L+++   E++DF+S+ 
Sbjct: 433 KVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK-IEVIDFSSYI 492

Query: 501 DLADQPGHYVIFWEVKGHVDDTLLGKCCSEMDAAFVDYGYVVSRRSNSIGPLELRIVERG 560
           D++  PGHY IFWE+ G  ++ +L  CC+ +D AF+D GYV SR+  +IG LELR+V +G
Sbjct: 493 DVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKG 552

Query: 561 TFNKILEHYIGNGAALSQFKTPRCTR--NQVLLNILNLSTIKTFRSTAY 608
           TF KI EH++G G++  QFK PRC +  N  +L IL  + + ++ STA+
Sbjct: 553 TFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 575

[Close]

BLAST of Lcy07g012510 vs. TAIR 10
Match: AT2G46370.4 (Auxin-responsive GH3 family protein )

HSP 1 Score: 570.5 bits (1469), Expect = 1.6e-162
Identity = 286/589 (48.56%), Postives = 392/589 (66.55%), Query Frame = 0


Query: 21  MIDWFEQVSSEAGVAQSRTLREILRENCGVEYLKQWLGDVNIHEITDDFALESLFTSLVP 80
           +ID F++++  A   Q +TL+EIL +N    YL+    + N    TD    E  F S+VP
Sbjct: 24  VIDEFDEMTRNAHQVQKQTLKEILLKNQSAIYLQNCGLNGN---ATDP---EEAFKSMVP 83

Query: 81  LSSHADLEPYLQRIADGDSAPLLTQQPINTLSLSSGTTEGRQKYVPFTPHSAQTTLLIFR 140
           L +  +LEPY++R+ DGD++P+LT  P+  +SLSSGT++GR K++PFT    + TL +FR
Sbjct: 84  LVTDVELEPYIKRMVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLFR 143

Query: 141 LAAAYRSRVYPTREGGKILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLETKSFT 200
            A A+R+R +P  + GK L+FI+SSKQ  + GG+  GTATT+ Y +  FK       S +
Sbjct: 144 TAFAFRNRDFPIDDNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSPS 203

Query: 201 CSPQEVISGGDYKQSTYCHLLLGLFFSDQVEYVTSTFAYSIVQAFNELEEAWPEICHDIT 260
           CSP EVI   D  Q+ YCHLL G+ F DQV+YV + FA+ +V AF   E+ W EI  DI 
Sbjct: 204 CSPDEVIFSPDVHQALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDIK 263

Query: 261 HGTLSSRITLPKIRKAVLNVIRPNPGLAAEIRGQCEELEGEDWLGLIPKLWRNCKYVYSI 320
            G LS+RIT+P +R A+  ++ PNP LA  IR +C  L   +W GLIP L+ N KYVY I
Sbjct: 264 DGVLSNRITVPSVRTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIPALFPNAKYVYGI 323

Query: 321 MTGSMQPYLKKLRHYAGGLALVSADYGSTESWIGVNVEPGLPPENVTFAVIPTFAYFEFI 380
           MTGSM+PY+ KLRHYAG L LVS DYGS+E WI  NV P L PE  TFAVIP   YFEF+
Sbjct: 324 MTGSMEPYVPKLRHYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEFL 383

Query: 381 PLFRQHQQQQHYCCGSSDIATDDFLEGQPVPLSQVKIGQEYELVLTTFTGLYRCRLGDVV 440
           P+    + ++                 +PV L+QVKIG+EYE+V+T + GLYR RLGDVV
Sbjct: 384 PVSETGEGEE-----------------KPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVV 443

Query: 441 EVAGFYNKTPKLNFICRRKLVLTINIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSHA 500
           +V GFYN TP+L FICRR L+L+INIDKNTE+DLQL VE  ++ L+++   E++DF+S+ 
Sbjct: 444 KVIGFYNNTPQLKFICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK-IEVIDFSSYI 503

Query: 501 DLADQPGHYVIFWEVKGHVDDTLLGKCCSEMDAAFVDYGYVVSRRSNSIGPLELRIVERG 560
           D++  PGHY IFWE+ G  ++ +L  CC+ +D AF+D GYV SR+  +IG LELR+V +G
Sbjct: 504 DVSTDPGHYAIFWEISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKG 563

Query: 561 TFNKILEHYIGNGAALSQFKTPRCTR--NQVLLNILNLSTIKTFRSTAY 608
           TF KI EH++G G++  QFK PRC +  N  +L IL  + + ++ STA+
Sbjct: 564 TFRKIQEHFLGLGSSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 586

[Close]

BLAST of Lcy07g012510 vs. TAIR 10
Match: AT2G46370.3 (Auxin-responsive GH3 family protein )

HSP 1 Score: 531.2 bits (1367), Expect = 1.1e-150
Identity = 259/516 (50.19%), Postives = 350/516 (67.83%), Query Frame = 0


Query: 94  IADGDSAPLLTQQPINTLSLSSGTTEGRQKYVPFTPHSAQTTLLIFRLAAAYRSRVYPTR 153
           + DGD++P+LT  P+  +SLSSGT++GR K++PFT    + TL +FR A A+R+R +P  
Sbjct: 1   MVDGDTSPILTGHPVPAISLSSGTSQGRPKFIPFTDELMENTLQLFRTAFAFRNRDFPID 60

Query: 154 EGGKILEFIYSSKQTKTKGGITTGTATTHYYASEQFKIKQLETKSFTCSPQEVISGGDYK 213
           + GK L+FI+SSKQ  + GG+  GTATT+ Y +  FK       S +CSP EVI   D  
Sbjct: 61  DNGKALQFIFSSKQYISTGGVPVGTATTNVYRNPNFKAGMKSITSPSCSPDEVIFSPDVH 120

Query: 214 QSTYCHLLLGLFFSDQVEYVTSTFAYSIVQAFNELEEAWPEICHDITHGTLSSRITLPKI 273
           Q+ YCHLL G+ F DQV+YV + FA+ +V AF   E+ W EI  DI  G LS+RIT+P +
Sbjct: 121 QALYCHLLSGILFRDQVQYVFAVFAHGLVHAFRTFEQVWEEIVTDIKDGVLSNRITVPSV 180

Query: 274 RKAVLNVIRPNPGLAAEIRGQCEELEGEDWLGLIPKLWRNCKYVYSIMTGSMQPYLKKLR 333
           R A+  ++ PNP LA  IR +C  L   +W GLIP L+ N KYVY IMTGSM+PY+ KLR
Sbjct: 181 RTAMSKLLTPNPELAETIRTKCMSL--SNWYGLIPALFPNAKYVYGIMTGSMEPYVPKLR 240

Query: 334 HYAGGLALVSADYGSTESWIGVNVEPGLPPENVTFAVIPTFAYFEFIPLFRQHQQQQHYC 393
           HYAG L LVS DYGS+E WI  NV P L PE  TFAVIP   YFEF+P+    + ++   
Sbjct: 241 HYAGDLPLVSHDYGSSEGWIAANVTPRLSPEEATFAVIPNLGYFEFLPVSETGEGEE--- 300

Query: 394 CGSSDIATDDFLEGQPVPLSQVKIGQEYELVLTTFTGLYRCRLGDVVEVAGFYNKTPKLN 453
                         +PV L+QVKIG+EYE+V+T + GLYR RLGDVV+V GFYN TP+L 
Sbjct: 301 --------------KPVGLTQVKIGEEYEVVITNYAGLYRYRLGDVVKVIGFYNNTPQLK 360

Query: 454 FICRRKLVLTINIDKNTEKDLQLVVERGSQLLADQSGAELVDFTSHADLADQPGHYVIFW 513
           FICRR L+L+INIDKNTE+DLQL VE  ++ L+++   E++DF+S+ D++  PGHY IFW
Sbjct: 361 FICRRNLILSINIDKNTERDLQLSVESAAKRLSEEK-IEVIDFSSYIDVSTDPGHYAIFW 420

Query: 514 EVKGHVDDTLLGKCCSEMDAAFVDYGYVVSRRSNSIGPLELRIVERGTFNKILEHYIGNG 573
           E+ G  ++ +L  CC+ +D AF+D GYV SR+  +IG LELR+V +GTF KI EH++G G
Sbjct: 421 EISGETNEDVLQDCCNCLDRAFIDAGYVSSRKCKTIGALELRVVAKGTFRKIQEHFLGLG 480

Query: 574 AALSQFKTPRCTR--NQVLLNILNLSTIKTFRSTAY 608
           ++  QFK PRC +  N  +L IL  + + ++ STA+
Sbjct: 481 SSAGQFKMPRCVKPSNAKVLQILCENVVSSYFSTAF 496

The following BLAST results are available for this feature:

BLAST of Lcy07g012510 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZNS2	7.8e-242	69.80	Indole-3-acetic acid-amido synthetase GH3.10 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
A0A1J6KGJ9	1.1e-176	52.12	Jasmonoyl--L-amino acid synthetase JAR4 OS=Nicotiana attenuata OX=49451 GN=JAR4 ...	[more]
A0A314KSQ4	6.3e-175	52.28	Jasmonoyl--L-amino acid synthetase JAR6 OS=Nicotiana attenuata OX=49451 GN=JAR6 ...	[more]
Q6I581	1.8e-166	49.41	Jasmonoyl--L-amino acid synthetase GH3.5 OS=Oryza sativa subsp. japonica OX=3994...	[more]
Q9SKE2	2.3e-161	48.56	Jasmonoyl--L-amino acid synthetase JAR1 OS=Arabidopsis thaliana OX=3702 GN=JAR1 ...	[more]

BLAST of Lcy07g012510 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J741	0.0e+00	88.29	indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1FRQ1	1.1e-309	87.95	indole-3-acetic acid-amido synthetase GH3.10 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1CHT7	6.5e-292	79.61	indole-3-acetic acid-amido synthetase GH3.10 OS=Momordica charantia OX=3673 GN=L...	[more]
A0A5D3B9T4	2.7e-290	82.57	Jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S3BIL6	2.7e-290	82.57	jasmonic acid-amido synthetase JAR1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103...	[more]

BLAST of Lcy07g012510 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022986272.1	0.0e+00	88.29	indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita maxima]	[more]
KAG6570848.1	2.3e-309	87.95	Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022943441.1	2.3e-309	87.95	indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita moschata]	[more]
XP_023512179.1	5.6e-309	87.95	indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita pepo subsp. pepo]	[more]
KAG7010696.1	8.7e-307	85.62	Indole-3-acetic acid-amido synthetase GH3.10 [Cucurbita argyrosperma subsp. argy...	[more]

BLAST of Lcy07g012510 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G03400.1	5.5e-243	69.80	Auxin-responsive GH3 family protein	[more]
AT2G46370.1	1.6e-162	48.56	Auxin-responsive GH3 family protein	[more]
AT2G46370.2	1.6e-162	48.56	Auxin-responsive GH3 family protein	[more]
AT2G46370.4	1.6e-162	48.56	Auxin-responsive GH3 family protein	[more]
AT2G46370.3	1.1e-150	50.19	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 24..588 e-value: 1.2E-179 score: 598.6
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 16..607
None	No IPR available	PANTHER	PTHR31901:SF48	INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE GH3.10	coord: 16..607

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy07g012510.1	Lcy07g012510.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016881	acid-amino acid ligase activity