Lcy07g005560 (gene) Sponge gourd (P93075) v1

Overview
NameLcy07g005560
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionN-acetyltransferase domain-containing protein
LocationChr07: 29208110 .. 29209860 (+)
RNA-Seq ExpressionLcy07g005560
SyntenyLcy07g005560
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACACACAACAGTTGAAACGTGAACACACACAAATTTTTCTTTTTTCCTTTTCTAAACCACAAAACTTTCAAGTTACAAAGCAACAAAAGTTAACCCAAAATCCCTCTACTACCTCAAATCCGCCATGGCAGAGCTGAAGAAGAGAAAGAAGAAGCTCGAGATTCTGATCAGAGAGTTCGACCCCATTAAAGACAGAGCTGGAGTTGAAGATGTCGAACGACGATGTGAAGTTGGATCGACAAAAAACAAAAAGCTGACTCTCTTCACCGATCTCCTTGGCGACCCAATTTGCAGAGTCCGCCATTCCCCTGCCTTCCTCATGCTCGTAAAGCTCTTTCCCCTTTTAATTCTGATCCTGAAATTGAACAAAAACACTCAAACAAGTCGTTCCTTCAGGTCGCAGAAATTGATGCCGACAAGGAGATTGTCGGCATGATCAGAGGCTGCATCAAGACTGTCAGTTGCTGCTCCAAACTCCCCAGAAATGGCGGAATCAAGGGCTCTGCTCTCATCCAACACCTCCCTGTTTACACTAAACTTGCTTACATCTTAGGCCTTCGGGTCTCTCCAGCTCATCGGTAACAAACAATTCTGTACATTATTTTGAAGTTGAATCAAACGGCTGTTATAATTCGATATTGAATTTGCAGGAGATTGGGGATTGGGATGAAGCTGGTGAGGCGAATGGAGGAGTGGTTCAATGAAAACAAAGCGGAATATTCATACATAGCGACAGAGAGTGACAACGTGGCTTCAATTAAGCTATTCATCGAGATATGCGGGTATTCCAAGTTCCGCACGCCGTCGATTCTCGTAAACCCGGTTTTCGCCCACCGGCTCCCCCTCTCGGACCGAGTCACCATCCTCCAACTCGGCTTGGCCGACGCCGAGACGCTGTACCGACGTCGGTTCGCGACGGCGGAGTTCTTCCCTCTCGACATCGACGCGGTGCTCTGCAACCGGTTGAGCCTGGGCACATTTCTAGCAGTGCCACGTGGGAGTTCGTGGGTCGGGTCGGATCGGTTGCCCGAGTCGTGGGCAATTCTGAGCGCGTGGAACTGCAAGGACATGTACGCACTGGAGGTGCGTGGCGCGTCGGCGACGAAGCGAGCAGTGGCGAAAATCAGCCGCATGATCGACCGAGGGCTGCCGTGGTTGCGAGTGCCGTCGGTACCGGAGGTGTTCTCGCCGTTCGGAGTGCTGTTTCTGTACGGAGTAGGAGGGGAAGGCCCGCACGCGGGGAAGCTGATGAAGGCGCTTTGCAACCATGCGCACAATCTGGCCAAGGAGCGTGGCTGCGGAGTGGTGGCCACGGAGGTTTCAAACGACGAGCCGCTCAAATCCTACATTCCGCATTGGAAAAAACTGTCTTGCCCTGAGGATTTATGGTGTATTAAGCGCCTTAGGGAAGGCTACGCCGACAACTCCATCGGTGACTGGACCAAATCTCCACCTGCCTTCTCCATATTTGTCGATCCTAGAGAATTCTAACGCATCAAAAAAAAAAATGAAAAACTGTGGGTGTTATATGAACTTTCTCCAATCTCTTTCAAATTAAAATTAAAGAAATTGGTAGTTAAGTTAAGGCAACCAAGATAAGGGGCTATTTAAGTTGCTACAATTGTATCTGTTTTTAATGGTTGTAATAGTGCACACAGACCCACAATAGTACGATCGTATAGTTCTTTTAGTACTTCACCTTAGTTGGATGACTACTTTCATCTCATGTTGTGCATTCTACTTGTCCATA

mRNA sequence

CACACACAACAGTTGAAACGTGAACACACACAAATTTTTCTTTTTTCCTTTTCTAAACCACAAAACTTTCAAGTTACAAAGCAACAAAAGTTAACCCAAAATCCCTCTACTACCTCAAATCCGCCATGGCAGAGCTGAAGAAGAGAAAGAAGAAGCTCGAGATTCTGATCAGAGAGTTCGACCCCATTAAAGACAGAGCTGGAGTTGAAGATGTCGAACGACGATGTGAAGTTGGATCGACAAAAAACAAAAAGCTGACTCTCTTCACCGATCTCCTTGGCGACCCAATTTGCAGAGTCCGCCATTCCCCTGCCTTCCTCATGCTCGTCGCAGAAATTGATGCCGACAAGGAGATTGTCGGCATGATCAGAGGCTGCATCAAGACTGTCAGTTGCTGCTCCAAACTCCCCAGAAATGGCGGAATCAAGGGCTCTGCTCTCATCCAACACCTCCCTGTTTACACTAAACTTGCTTACATCTTAGGCCTTCGGGTCTCTCCAGCTCATCGGAGATTGGGGATTGGGATGAAGCTGGTGAGGCGAATGGAGGAGTGGTTCAATGAAAACAAAGCGGAATATTCATACATAGCGACAGAGAGTGACAACGTGGCTTCAATTAAGCTATTCATCGAGATATGCGGGTATTCCAAGTTCCGCACGCCGTCGATTCTCGTAAACCCGGTTTTCGCCCACCGGCTCCCCCTCTCGGACCGAGTCACCATCCTCCAACTCGGCTTGGCCGACGCCGAGACGCTGTACCGACGTCGGTTCGCGACGGCGGAGTTCTTCCCTCTCGACATCGACGCGGTGCTCTGCAACCGGTTGAGCCTGGGCACATTTCTAGCAGTGCCACGTGGGAGTTCGTGGGTCGGGTCGGATCGGTTGCCCGAGTCGTGGGCAATTCTGAGCGCGTGGAACTGCAAGGACATGTACGCACTGGAGGTGCGTGGCGCGTCGGCGACGAAGCGAGCAGTGGCGAAAATCAGCCGCATGATCGACCGAGGGCTGCCGTGGTTGCGAGTGCCGTCGGTACCGGAGGTGTTCTCGCCGTTCGGAGTGCTGTTTCTGTACGGAGTAGGAGGGGAAGGCCCGCACGCGGGGAAGCTGATGAAGGCGCTTTGCAACCATGCGCACAATCTGGCCAAGGAGCGTGGCTGCGGAGTGGTGGCCACGGAGGTTTCAAACGACGAGCCGCTCAAATCCTACATTCCGCATTGGAAAAAACTGTCTTGCCCTGAGGATTTATGGTGTATTAAGCGCCTTAGGGAAGGCTACGCCGACAACTCCATCGGTGACTGGACCAAATCTCCACCTGCCTTCTCCATATTTGTCGATCCTAGAGAATTCTAACGCATCAAAAAAAAAAATGAAAAACTGTGGGTGTTATATGAACTTTCTCCAATCTCTTTCAAATTAAAATTAAAGAAATTGGTAGTTAAGTTAAGGCAACCAAGATAAGGGGCTATTTAAGTTGCTACAATTGTATCTGTTTTTAATGGTTGTAATAGTGCACACAGACCCACAATAGTACGATCGTATAGTTCTTTTAGTACTTCACCTTAGTTGGATGACTACTTTCATCTCATGTTGTGCATTCTACTTGTCCATA

Coding sequence (CDS)

ATGGCAGAGCTGAAGAAGAGAAAGAAGAAGCTCGAGATTCTGATCAGAGAGTTCGACCCCATTAAAGACAGAGCTGGAGTTGAAGATGTCGAACGACGATGTGAAGTTGGATCGACAAAAAACAAAAAGCTGACTCTCTTCACCGATCTCCTTGGCGACCCAATTTGCAGAGTCCGCCATTCCCCTGCCTTCCTCATGCTCGTCGCAGAAATTGATGCCGACAAGGAGATTGTCGGCATGATCAGAGGCTGCATCAAGACTGTCAGTTGCTGCTCCAAACTCCCCAGAAATGGCGGAATCAAGGGCTCTGCTCTCATCCAACACCTCCCTGTTTACACTAAACTTGCTTACATCTTAGGCCTTCGGGTCTCTCCAGCTCATCGGAGATTGGGGATTGGGATGAAGCTGGTGAGGCGAATGGAGGAGTGGTTCAATGAAAACAAAGCGGAATATTCATACATAGCGACAGAGAGTGACAACGTGGCTTCAATTAAGCTATTCATCGAGATATGCGGGTATTCCAAGTTCCGCACGCCGTCGATTCTCGTAAACCCGGTTTTCGCCCACCGGCTCCCCCTCTCGGACCGAGTCACCATCCTCCAACTCGGCTTGGCCGACGCCGAGACGCTGTACCGACGTCGGTTCGCGACGGCGGAGTTCTTCCCTCTCGACATCGACGCGGTGCTCTGCAACCGGTTGAGCCTGGGCACATTTCTAGCAGTGCCACGTGGGAGTTCGTGGGTCGGGTCGGATCGGTTGCCCGAGTCGTGGGCAATTCTGAGCGCGTGGAACTGCAAGGACATGTACGCACTGGAGGTGCGTGGCGCGTCGGCGACGAAGCGAGCAGTGGCGAAAATCAGCCGCATGATCGACCGAGGGCTGCCGTGGTTGCGAGTGCCGTCGGTACCGGAGGTGTTCTCGCCGTTCGGAGTGCTGTTTCTGTACGGAGTAGGAGGGGAAGGCCCGCACGCGGGGAAGCTGATGAAGGCGCTTTGCAACCATGCGCACAATCTGGCCAAGGAGCGTGGCTGCGGAGTGGTGGCCACGGAGGTTTCAAACGACGAGCCGCTCAAATCCTACATTCCGCATTGGAAAAAACTGTCTTGCCCTGAGGATTTATGGTGTATTAAGCGCCTTAGGGAAGGCTACGCCGACAACTCCATCGGTGACTGGACCAAATCTCCACCTGCCTTCTCCATATTTGTCGATCCTAGAGAATTCTAA

Protein sequence

MAELKKRKKKLEILIREFDPIKDRAGVEDVERRCEVGSTKNKKLTLFTDLLGDPICRVRHSPAFLMLVAEIDADKEIVGMIRGCIKTVSCCSKLPRNGGIKGSALIQHLPVYTKLAYILGLRVSPAHRRLGIGMKLVRRMEEWFNENKAEYSYIATESDNVASIKLFIEICGYSKFRTPSILVNPVFAHRLPLSDRVTILQLGLADAETLYRRRFATAEFFPLDIDAVLCNRLSLGTFLAVPRGSSWVGSDRLPESWAILSAWNCKDMYALEVRGASATKRAVAKISRMIDRGLPWLRVPSVPEVFSPFGVLFLYGVGGEGPHAGKLMKALCNHAHNLAKERGCGVVATEVSNDEPLKSYIPHWKKLSCPEDLWCIKRLREGYADNSIGDWTKSPPAFSIFVDPREF
Homology
BLAST of Lcy07g005560 vs. ExPASy Swiss-Prot
Match: Q42381 (Probable N-acetyltransferase HLS1 OS=Arabidopsis thaliana OX=3702 GN=HLS1 PE=1 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 4.1e-154
Identity = 264/406 (65.02%), Postives = 320/406 (78.82%), Query Frame = 0

Query: 14  LIREFDPIKDRAGVEDVERRCEVGSTKNKKLTLFTDLLGDPICRVRHSPAFLMLVAEIDA 73
           ++RE+DP +D  GVEDVERRCEVG   + KL+LFTDLLGDPICR+RHSP++LMLVAE+  
Sbjct: 3   VVREYDPTRDLVGVEDVERRCEVG--PSGKLSLFTDLLGDPICRIRHSPSYLMLVAEMGT 62

Query: 74  D-KEIVGMIRGCIKTVSCCSKLPRNGGIKGSALIQHLPVYTKLAYILGLRVSPAHRRLGI 133
           + KEIVGMIRGCIKTV+C  KL  N   +   +    P+YTKLAY+LGLRVSP HRR GI
Sbjct: 63  EKKEIVGMIRGCIKTVTCGQKLDLNHKSQNDVV---KPLYTKLAYVLGLRVSPFHRRQGI 122

Query: 134 GMKLVRRMEEWFNENKAEYSYIATESDNVASIKLFIEICGYSKFRTPSILVNPVFAHRLP 193
           G KLV+ MEEWF +N AEYSYIATE+DN AS+ LF   CGYS+FRTPSILVNPV+AHR+ 
Sbjct: 123 GFKLVKMMEEWFRQNGAEYSYIATENDNQASVNLFTGKCGYSEFRTPSILVNPVYAHRVN 182

Query: 194 LSDRVTILQLGLADAETLYRRRFATAEFFPLDIDAVLCNRLSLGTFLAVPRGS------- 253
           +S RVT+++L   DAETLYR RF+T EFFP DID+VL N+LSLGTF+AVPRGS       
Sbjct: 183 VSRRVTVIKLEPVDAETLYRIRFSTTEFFPRDIDSVLNNKLSLGTFVAVPRGSCYGSGSG 242

Query: 254 SWVGSDRL----PESWAILSAWNCKDMYALEVRGASATKRAVAKISRMIDRGLPWLRVPS 313
           SW GS +     PESWA+LS WNCKD + LEVRGAS  +R VAK +R++D+ LP+L++PS
Sbjct: 243 SWPGSAKFLEYPPESWAVLSVWNCKDSFLLEVRGASRLRRVVAKTTRVVDKTLPFLKLPS 302

Query: 314 VPEVFSPFGVLFLYGVGGEGPHAGKLMKALCNHAHNLAKERGCGVVATEVSNDEPLKSYI 373
           +P VF PFG+ F+YG+GGEGP A K++K+LC HAHNLAK  GCGVVA EV+ ++PL+  I
Sbjct: 303 IPSVFEPFGLHFMYGIGGEGPRAVKMVKSLCAHAHNLAKAGGCGVVAAEVAGEDPLRRGI 362

Query: 374 PHWKKLSCPEDLWCIKRLREGYADNSIGDWTKSPPAFSIFVDPREF 408
           PHWK LSC EDLWCIKRL + Y+D  +GDWTKSPP  SIFVDPREF
Sbjct: 363 PHWKVLSCDEDLWCIKRLGDDYSDGVVGDWTKSPPGVSIFVDPREF 403

BLAST of Lcy07g005560 vs. ExPASy Swiss-Prot
Match: O64815 (Probable N-acetyltransferase HLS1-like OS=Arabidopsis thaliana OX=3702 GN=At2g23060 PE=2 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 2.7e-153
Identity = 265/409 (64.79%), Postives = 320/409 (78.24%), Query Frame = 0

Query: 15  IREFDPIKDRAGVEDVERRCEVGSTKNKKLTLFTDLLGDPICRVRHSPAFLMLVAEI--D 74
           +RE+DP KD A VEDVERRCEVG     KL+LFTDLLGDPICRVRHSP++LMLVAEI   
Sbjct: 7   VREYDPSKDLATVEDVERRCEVGPA--GKLSLFTDLLGDPICRVRHSPSYLMLVAEIGPK 66

Query: 75  ADKEIVGMIRGCIKTVSC---CSKLPRNGGIKGSALIQHLPVYTKLAYILGLRVSPAHRR 134
             KE+VGMIRGCIKTV+C     +L        + ++   P+YTKLAYILGLRVSP HRR
Sbjct: 67  EKKELVGMIRGCIKTVTCGITTKRLDLTHNKSQNDVVITKPLYTKLAYILGLRVSPTHRR 126

Query: 135 LGIGMKLVRRMEEWFNENKAEYSYIATESDNVASIKLFIEICGYSKFRTPSILVNPVFAH 194
            GIG KLV+ ME+WF++N AEYSY ATE+DN AS+ LF   CGY++FRTPSILVNPV+AH
Sbjct: 127 QGIGFKLVKAMEDWFSQNGAEYSYFATENDNHASVNLFTGKCGYAEFRTPSILVNPVYAH 186

Query: 195 RLPLSDRVTILQLGLADAETLYRRRFATAEFFPLDIDAVLCNRLSLGTFLAVPRGS---- 254
           R+ +S RVT+++L  +DAE LYR RF+T EFFP DID+VL N+LSLGTF+AVPRGS    
Sbjct: 187 RVNISRRVTVIKLEPSDAELLYRLRFSTTEFFPRDIDSVLNNKLSLGTFVAVPRGSCYGS 246

Query: 255 ---SWVGSDRL----PESWAILSAWNCKDMYALEVRGASATKRAVAKISRMIDRGLPWLR 314
              SW GS +     P+SWA+LS WNCKD + LEVRGAS  +R V+K +RM+D+ LP+L+
Sbjct: 247 GSRSWPGSAKFLEYPPDSWAVLSVWNCKDSFRLEVRGASRLRRVVSKATRMVDKTLPFLK 306

Query: 315 VPSVPEVFSPFGVLFLYGVGGEGPHAGKLMKALCNHAHNLAKERGCGVVATEVSNDEPLK 374
           +PS+P VF PFG+ F+YG+GGEGP A K++KALC+HAHNLAKE GCGVVA EV+ +EPL+
Sbjct: 307 IPSIPAVFRPFGLHFMYGIGGEGPRAEKMVKALCDHAHNLAKEGGCGVVAAEVAGEEPLR 366

Query: 375 SYIPHWKKLSCPEDLWCIKRLREGYADNSIGDWTKSPPAFSIFVDPREF 408
             IPHWK LSC EDLWCIKRL E Y+D S+GDWTKSPP  SIFVDPREF
Sbjct: 367 RGIPHWKVLSCAEDLWCIKRLGEDYSDGSVGDWTKSPPGDSIFVDPREF 413

BLAST of Lcy07g005560 vs. ExPASy TrEMBL
Match: A0A6J1F329 (probable N-acetyltransferase HLS1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111439465 PE=4 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 1.3e-195
Identity = 343/411 (83.45%), Postives = 360/411 (87.59%), Query Frame = 0

Query: 5   KKRKKKLEILIREFDPIKDRAGVEDVERRCEVGSTKNKKLTLFTDLLGDPICRVRHSPAF 64
           KKRK  ++ILIREFDPIKD+  VEDVERRCEVGSTKNK  +LFTDLLGDPICRVRHSPAF
Sbjct: 9   KKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPICRVRHSPAF 68

Query: 65  LMLVAEIDADKEIVGMIRGCIKTVSCCSKLPRNGGIKGSALIQHLPVYTKLAYILGLRVS 124
           LMLVAEI A  EIVGMIRGCIKTV+CCSK  RN GI  S L Q  PVYTKLAYILGLRVS
Sbjct: 69  LMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLAYILGLRVS 128

Query: 125 PAHRRLGIGMKLVRRMEEWFNENKAEYSYIATESDNVASIKLFIEICGYSKFRTPSILVN 184
           P HRRLGIG+ LVRRMEEWF E KAEYSYIATE DNVASIKLF   CGYSKFRTPSILVN
Sbjct: 129 PDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKFRTPSILVN 188

Query: 185 PVFAHRLPLSDRVTILQLGLADAETLYRRRFATAEFFPLDIDAVLCNRLSLGTFLAVPRG 244
           PVFAHRL LSD+VTILQL L  AETLYR RFATAEFFP DIDAVL NRLSLGTFLAVPRG
Sbjct: 189 PVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLGTFLAVPRG 248

Query: 245 S----SWVGSDR----LPESWAILSAWNCKDMYALEVRGASATKRAVAKISRMIDRGLPW 304
           S     WV SDR    LPESWA+LSAWNCKD+YALEVRG S  KRA+AK+SR+IDRGLPW
Sbjct: 249 SFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSRVIDRGLPW 308

Query: 305 LRVPSVPEVFSPFGVLFLYGVGGEGPHAGKLMKALCNHAHNLAKERGCGVVATEVSNDEP 364
           LR+PSVPEVF PFGVLFLYGVGGEGP AGKLMKALC+HAHNLAKERGCGVVATEVSNDEP
Sbjct: 309 LRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVATEVSNDEP 368

Query: 365 LKSYIPHWKKLSCPEDLWCIKRLREGYADNSIGDWTKSPPAFSIFVDPREF 408
           LKS IPHWKKLSCPEDLWCIKRL EGY +NS+GDWTKSPP+FSIFVDPREF
Sbjct: 369 LKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 419

BLAST of Lcy07g005560 vs. ExPASy TrEMBL
Match: A0A6J1EYB0 (probable N-acetyltransferase HLS1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111439465 PE=4 SV=1)

HSP 1 Score: 685.6 bits (1768), Expect = 1.2e-193
Identity = 342/411 (83.21%), Postives = 359/411 (87.35%), Query Frame = 0

Query: 5   KKRKKKLEILIREFDPIKDRAGVEDVERRCEVGSTKNKKLTLFTDLLGDPICRVRHSPAF 64
           KKRK  ++ILIREFDPIKD+  VEDVERRCEVGSTKNK  +LFTDLLGDPICRVRHSPAF
Sbjct: 9   KKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPICRVRHSPAF 68

Query: 65  LMLVAEIDADKEIVGMIRGCIKTVSCCSKLPRNGGIKGSALIQHLPVYTKLAYILGLRVS 124
           LMLVAEI A  EIVGMIRGCIKTV+CCSK  RN GI  S L Q  PVYTKLAYILGLRVS
Sbjct: 69  LMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLAYILGLRVS 128

Query: 125 PAHRRLGIGMKLVRRMEEWFNENKAEYSYIATESDNVASIKLFIEICGYSKFRTPSILVN 184
           P H RLGIG+ LVRRMEEWF E KAEYSYIATE DNVASIKLF   CGYSKFRTPSILVN
Sbjct: 129 PDH-RLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKFRTPSILVN 188

Query: 185 PVFAHRLPLSDRVTILQLGLADAETLYRRRFATAEFFPLDIDAVLCNRLSLGTFLAVPRG 244
           PVFAHRL LSD+VTILQL L  AETLYR RFATAEFFP DIDAVL NRLSLGTFLAVPRG
Sbjct: 189 PVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLGTFLAVPRG 248

Query: 245 S----SWVGSDR----LPESWAILSAWNCKDMYALEVRGASATKRAVAKISRMIDRGLPW 304
           S     WV SDR    LPESWA+LSAWNCKD+YALEVRG S  KRA+AK+SR+IDRGLPW
Sbjct: 249 SFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSRVIDRGLPW 308

Query: 305 LRVPSVPEVFSPFGVLFLYGVGGEGPHAGKLMKALCNHAHNLAKERGCGVVATEVSNDEP 364
           LR+PSVPEVF PFGVLFLYGVGGEGP AGKLMKALC+HAHNLAKERGCGVVATEVSNDEP
Sbjct: 309 LRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVATEVSNDEP 368

Query: 365 LKSYIPHWKKLSCPEDLWCIKRLREGYADNSIGDWTKSPPAFSIFVDPREF 408
           LKS IPHWKKLSCPEDLWCIKRL EGY +NS+GDWTKSPP+FSIFVDPREF
Sbjct: 369 LKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 418

BLAST of Lcy07g005560 vs. ExPASy TrEMBL
Match: A0A6J1JHA4 (probable N-acetyltransferase HLS1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484371 PE=4 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.8e-192
Identity = 339/411 (82.48%), Postives = 355/411 (86.37%), Query Frame = 0

Query: 5   KKRKKKLEILIREFDPIKDRAGVEDVERRCEVGSTKNKKLTLFTDLLGDPICRVRHSPAF 64
           KKRK  ++ILIREFDP KDR  VEDVERRCEVGSTKNK  +LFTDLLGDPICRVRHSPAF
Sbjct: 8   KKRKNNIQILIREFDPTKDRPAVEDVERRCEVGSTKNKNFSLFTDLLGDPICRVRHSPAF 67

Query: 65  LMLVAEIDADKEIVGMIRGCIKTVSCCSKLPRNGGIKGSALIQHLPVYTKLAYILGLRVS 124
           LMLVAEI A  EIVGMIRGCIKTV+CCSK  RN GI  S L    PVYTKLAYILGLRVS
Sbjct: 68  LMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNSGINASDLPHLAPVYTKLAYILGLRVS 127

Query: 125 PAHRRLGIGMKLVRRMEEWFNENKAEYSYIATESDNVASIKLFIEICGYSKFRTPSILVN 184
           P HRRLGIG+ LVRRMEEWF E KAEYSYIATE DNVASIKLF   CGY KFRTPSILVN
Sbjct: 128 PDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYFKFRTPSILVN 187

Query: 185 PVFAHRLPLSDRVTILQLGLADAETLYRRRFATAEFFPLDIDAVLCNRLSLGTFLAVPRG 244
           PVFAHRL LSD+VTIL+L L  AETLYR RFA AEFFP DIDAVL NRLSLGTFLAVPRG
Sbjct: 188 PVFAHRLRLSDQVTILRLELTVAETLYRCRFAAAEFFPRDIDAVLSNRLSLGTFLAVPRG 247

Query: 245 S----SWVGSDR----LPESWAILSAWNCKDMYALEVRGASATKRAVAKISRMIDRGLPW 304
           S     WV SDR    LPESWA+LSAWNCKD+YALEVRG S  KRA+AK SR+IDRGLPW
Sbjct: 248 SFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKFSRVIDRGLPW 307

Query: 305 LRVPSVPEVFSPFGVLFLYGVGGEGPHAGKLMKALCNHAHNLAKERGCGVVATEVSNDEP 364
           LR+PSVPEVF+PFGVLFLYGVGGEGP AGKLMKALC+HAHNLAKERGCGVVATEVSNDEP
Sbjct: 308 LRLPSVPEVFTPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVATEVSNDEP 367

Query: 365 LKSYIPHWKKLSCPEDLWCIKRLREGYADNSIGDWTKSPPAFSIFVDPREF 408
           LKS IPHWKKLSCPEDLWCIKRL E Y DNS+GDWTKSPP+FSIFVDPREF
Sbjct: 368 LKSDIPHWKKLSCPEDLWCIKRLGECYTDNSLGDWTKSPPSFSIFVDPREF 418

BLAST of Lcy07g005560 vs. ExPASy TrEMBL
Match: A0A6J1JGS4 (probable N-acetyltransferase HLS1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484371 PE=4 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 1.7e-190
Identity = 338/411 (82.24%), Postives = 354/411 (86.13%), Query Frame = 0

Query: 5   KKRKKKLEILIREFDPIKDRAGVEDVERRCEVGSTKNKKLTLFTDLLGDPICRVRHSPAF 64
           KKRK  ++ILIREFDP KDR  VEDVERRCEVGSTKNK  +LFTDLLGDPICRVRHSPAF
Sbjct: 8   KKRKNNIQILIREFDPTKDRPAVEDVERRCEVGSTKNKNFSLFTDLLGDPICRVRHSPAF 67

Query: 65  LMLVAEIDADKEIVGMIRGCIKTVSCCSKLPRNGGIKGSALIQHLPVYTKLAYILGLRVS 124
           LMLVAEI A  EIVGMIRGCIKTV+CCSK  RN GI  S L    PVYTKLAYILGLRVS
Sbjct: 68  LMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNSGINASDLPHLAPVYTKLAYILGLRVS 127

Query: 125 PAHRRLGIGMKLVRRMEEWFNENKAEYSYIATESDNVASIKLFIEICGYSKFRTPSILVN 184
           P H RLGIG+ LVRRMEEWF E KAEYSYIATE DNVASIKLF   CGY KFRTPSILVN
Sbjct: 128 PDH-RLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYFKFRTPSILVN 187

Query: 185 PVFAHRLPLSDRVTILQLGLADAETLYRRRFATAEFFPLDIDAVLCNRLSLGTFLAVPRG 244
           PVFAHRL LSD+VTIL+L L  AETLYR RFA AEFFP DIDAVL NRLSLGTFLAVPRG
Sbjct: 188 PVFAHRLRLSDQVTILRLELTVAETLYRCRFAAAEFFPRDIDAVLSNRLSLGTFLAVPRG 247

Query: 245 S----SWVGSDR----LPESWAILSAWNCKDMYALEVRGASATKRAVAKISRMIDRGLPW 304
           S     WV SDR    LPESWA+LSAWNCKD+YALEVRG S  KRA+AK SR+IDRGLPW
Sbjct: 248 SFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKFSRVIDRGLPW 307

Query: 305 LRVPSVPEVFSPFGVLFLYGVGGEGPHAGKLMKALCNHAHNLAKERGCGVVATEVSNDEP 364
           LR+PSVPEVF+PFGVLFLYGVGGEGP AGKLMKALC+HAHNLAKERGCGVVATEVSNDEP
Sbjct: 308 LRLPSVPEVFTPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVATEVSNDEP 367

Query: 365 LKSYIPHWKKLSCPEDLWCIKRLREGYADNSIGDWTKSPPAFSIFVDPREF 408
           LKS IPHWKKLSCPEDLWCIKRL E Y DNS+GDWTKSPP+FSIFVDPREF
Sbjct: 368 LKSDIPHWKKLSCPEDLWCIKRLGECYTDNSLGDWTKSPPSFSIFVDPREF 417

BLAST of Lcy07g005560 vs. ExPASy TrEMBL
Match: A0A5C7HQF5 (N-acetyltransferase domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_013870 PE=4 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 2.0e-164
Identity = 288/403 (71.46%), Postives = 333/403 (82.63%), Query Frame = 0

Query: 13  ILIREFDPIKDRAGVEDVERRCEVGSTKNKKLTLFTDLLGDPICRVRHSPAFLMLVAEID 72
           +++R FD  KDR GVEDVERRCEVG   + KL LFTDLLGDPICRVR+SPAFLMLVAEI 
Sbjct: 11  VVVRAFDENKDRFGVEDVERRCEVG--PSGKLCLFTDLLGDPICRVRNSPAFLMLVAEI- 70

Query: 73  ADKEIVGMIRGCIKTVSCCSKLPRNGGIKGSALIQHLPVYTKLAYILGLRVSPAHRRLGI 132
            D+EIVGMIRGCIKTV+C  KL RNG  K +   +HLPVYTK+AYILGLRVSP+HRR+GI
Sbjct: 71  -DEEIVGMIRGCIKTVTCGKKLSRNG--KNNNQNKHLPVYTKVAYILGLRVSPSHRRMGI 130

Query: 133 GMKLVRRMEEWFNENKAEYSYIATESDNVASIKLFIEICGYSKFRTPSILVNPVFAHRLP 192
           G+KLV R+EEWF E  AEYSYIATE+DN AS+KLF + CGYSKFRTPSILVNPVFAHRL 
Sbjct: 131 GLKLVTRIEEWFRETGAEYSYIATENDNYASVKLFTDKCGYSKFRTPSILVNPVFAHRLA 190

Query: 193 LSDRVTILQLGLADAETLYRRRFATAEFFPLDIDAVLCNRLSLGTFLAVPRGS----SWV 252
           +S +VT++ LG +DAE LYRR+F+T EFFP DID+VL N+L+LGTFLAVPRG+    SW 
Sbjct: 191 VSGKVTVIGLGPSDAEALYRRKFSTTEFFPRDIDSVLNNKLTLGTFLAVPRGTYTPESWP 250

Query: 253 GSDRL----PESWAILSAWNCKDMYALEVRGASATKRAVAKISRMIDRGLPWLRVPSVPE 312
           GSD      PESWA+LS WNC D++ LEVRGAS  +  +AK SR++DR  PWLR+PSVPE
Sbjct: 251 GSDLFLSNPPESWAVLSVWNCNDVFKLEVRGASRVQLTLAKTSRIVDRFFPWLRIPSVPE 310

Query: 313 VFSPFGVLFLYGVGGEGPHAGKLMKALCNHAHNLAKERGCGVVATEVSNDEPLKSYIPHW 372
           VFSPFG+ F+YG+GGEGP A K++KALC +AHNLAKE GCGVVATEVS+ EPLK  IPHW
Sbjct: 311 VFSPFGLHFMYGLGGEGPRAVKMVKALCGYAHNLAKESGCGVVATEVSSGEPLKLGIPHW 370

Query: 373 KKLSCPEDLWCIKRLREGYADNSIGDWTKSPPAFSIFVDPREF 408
           KKLSC EDLWCIKRL E Y+D S+GDWTKSPP  SIFVDPREF
Sbjct: 371 KKLSCAEDLWCIKRLGEDYSDGSVGDWTKSPPGLSIFVDPREF 407

BLAST of Lcy07g005560 vs. NCBI nr
Match: XP_023512928.1 (probable N-acetyltransferase HLS1 [Cucurbita pepo subsp. pepo] >XP_023522343.1 probable N-acetyltransferase HLS1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 694.1 bits (1790), Expect = 7.1e-196
Identity = 344/411 (83.70%), Postives = 361/411 (87.83%), Query Frame = 0

Query: 5   KKRKKKLEILIREFDPIKDRAGVEDVERRCEVGSTKNKKLTLFTDLLGDPICRVRHSPAF 64
           KKRK  ++ILIREFDPIKDR  VEDVERRCEVGSTKNK  +LFTDLLGDPICRVRHSPAF
Sbjct: 10  KKRKNNIQILIREFDPIKDRTAVEDVERRCEVGSTKNKNFSLFTDLLGDPICRVRHSPAF 69

Query: 65  LMLVAEIDADKEIVGMIRGCIKTVSCCSKLPRNGGIKGSALIQHLPVYTKLAYILGLRVS 124
           LMLVAEI A  EIVGMIRGCIKTV+CCSK  RN GI  S L Q  PVYTKLAYILGLRVS
Sbjct: 70  LMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLAYILGLRVS 129

Query: 125 PAHRRLGIGMKLVRRMEEWFNENKAEYSYIATESDNVASIKLFIEICGYSKFRTPSILVN 184
           P HRRLGIG+ LVRRMEEWF E KAEYSYIATE DNVASIKLF   CGYSKFRTPSILVN
Sbjct: 130 PDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKFRTPSILVN 189

Query: 185 PVFAHRLPLSDRVTILQLGLADAETLYRRRFATAEFFPLDIDAVLCNRLSLGTFLAVPRG 244
           PVFAHRL LSD+VTILQL L  AETLYR RFATAEFFP DIDAVL NRLSLGTFLAVPRG
Sbjct: 190 PVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLGTFLAVPRG 249

Query: 245 S----SWVGSDR----LPESWAILSAWNCKDMYALEVRGASATKRAVAKISRMIDRGLPW 304
           S     WV SDR    LPESWA+LSAWNCKD+YALEVRG S  KRA+AK+SR+IDRGLPW
Sbjct: 250 SFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSRVIDRGLPW 309

Query: 305 LRVPSVPEVFSPFGVLFLYGVGGEGPHAGKLMKALCNHAHNLAKERGCGVVATEVSNDEP 364
           LR+PSVPEVF+PFGVLFLYGVGGEGP AGKLMKALC+HAHNLAKERGCGVVATEVSNDEP
Sbjct: 310 LRLPSVPEVFTPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVATEVSNDEP 369

Query: 365 LKSYIPHWKKLSCPEDLWCIKRLREGYADNSIGDWTKSPPAFSIFVDPREF 408
           LKS IPHWKKLSCPEDLWCIKRL EGY +NS+GDWTKSPP+FSIFVDPREF
Sbjct: 370 LKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 420

BLAST of Lcy07g005560 vs. NCBI nr
Match: XP_022932903.1 (probable N-acetyltransferase HLS1 isoform X1 [Cucurbita moschata] >KAG7011045.1 putative N-acetyltransferase HLS1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 692.2 bits (1785), Expect = 2.7e-195
Identity = 343/411 (83.45%), Postives = 360/411 (87.59%), Query Frame = 0

Query: 5   KKRKKKLEILIREFDPIKDRAGVEDVERRCEVGSTKNKKLTLFTDLLGDPICRVRHSPAF 64
           KKRK  ++ILIREFDPIKD+  VEDVERRCEVGSTKNK  +LFTDLLGDPICRVRHSPAF
Sbjct: 9   KKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPICRVRHSPAF 68

Query: 65  LMLVAEIDADKEIVGMIRGCIKTVSCCSKLPRNGGIKGSALIQHLPVYTKLAYILGLRVS 124
           LMLVAEI A  EIVGMIRGCIKTV+CCSK  RN GI  S L Q  PVYTKLAYILGLRVS
Sbjct: 69  LMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLAYILGLRVS 128

Query: 125 PAHRRLGIGMKLVRRMEEWFNENKAEYSYIATESDNVASIKLFIEICGYSKFRTPSILVN 184
           P HRRLGIG+ LVRRMEEWF E KAEYSYIATE DNVASIKLF   CGYSKFRTPSILVN
Sbjct: 129 PDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKFRTPSILVN 188

Query: 185 PVFAHRLPLSDRVTILQLGLADAETLYRRRFATAEFFPLDIDAVLCNRLSLGTFLAVPRG 244
           PVFAHRL LSD+VTILQL L  AETLYR RFATAEFFP DIDAVL NRLSLGTFLAVPRG
Sbjct: 189 PVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLGTFLAVPRG 248

Query: 245 S----SWVGSDR----LPESWAILSAWNCKDMYALEVRGASATKRAVAKISRMIDRGLPW 304
           S     WV SDR    LPESWA+LSAWNCKD+YALEVRG S  KRA+AK+SR+IDRGLPW
Sbjct: 249 SFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSRVIDRGLPW 308

Query: 305 LRVPSVPEVFSPFGVLFLYGVGGEGPHAGKLMKALCNHAHNLAKERGCGVVATEVSNDEP 364
           LR+PSVPEVF PFGVLFLYGVGGEGP AGKLMKALC+HAHNLAKERGCGVVATEVSNDEP
Sbjct: 309 LRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVATEVSNDEP 368

Query: 365 LKSYIPHWKKLSCPEDLWCIKRLREGYADNSIGDWTKSPPAFSIFVDPREF 408
           LKS IPHWKKLSCPEDLWCIKRL EGY +NS+GDWTKSPP+FSIFVDPREF
Sbjct: 369 LKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 419

BLAST of Lcy07g005560 vs. NCBI nr
Match: KAG6571244.1 (putative N-acetyltransferase HLS1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 688.7 bits (1776), Expect = 3.0e-194
Identity = 341/411 (82.97%), Postives = 360/411 (87.59%), Query Frame = 0

Query: 5   KKRKKKLEILIREFDPIKDRAGVEDVERRCEVGSTKNKKLTLFTDLLGDPICRVRHSPAF 64
           KKRK  ++ILIREFDPIKD+  VEDVERRCEVGSTKNK  +LFTDLLGDPICRVRHSPAF
Sbjct: 8   KKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPICRVRHSPAF 67

Query: 65  LMLVAEIDADKEIVGMIRGCIKTVSCCSKLPRNGGIKGSALIQHLPVYTKLAYILGLRVS 124
           LMLVAEI A  EIVGMIRGCIKTV+CCSK  RN GI  S L Q  PVYTKLAYILGLRVS
Sbjct: 68  LMLVAEISAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQIAPVYTKLAYILGLRVS 127

Query: 125 PAHRRLGIGMKLVRRMEEWFNENKAEYSYIATESDNVASIKLFIEICGYSKFRTPSILVN 184
           P HRRLGIG+ LVRRMEEWF E +AEYSYIATE DNVASIKLF   CGYSKFRTPSILVN
Sbjct: 128 PDHRRLGIGLNLVRRMEEWFREKRAEYSYIATEIDNVASIKLFTHKCGYSKFRTPSILVN 187

Query: 185 PVFAHRLPLSDRVTILQLGLADAETLYRRRFATAEFFPLDIDAVLCNRLSLGTFLAVPRG 244
           PVFAHRL LSD+VTILQL L  AETLYR RFATAEFFP DIDAVL NRLSLGTFLAVPRG
Sbjct: 188 PVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLGTFLAVPRG 247

Query: 245 S----SWVGSDR----LPESWAILSAWNCKDMYALEVRGASATKRAVAKISRMIDRGLPW 304
           S     WV SDR    LPESWA+LSAWNCKD+YALEVRG S  KRA+AK+SR+IDR LPW
Sbjct: 248 SFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSRVIDRVLPW 307

Query: 305 LRVPSVPEVFSPFGVLFLYGVGGEGPHAGKLMKALCNHAHNLAKERGCGVVATEVSNDEP 364
           LR+PSVPEVF+PFGVLFLYGVGGEGP AGKLMKALC+HAHNLAKERGCGVVATEVSNDEP
Sbjct: 308 LRLPSVPEVFTPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVATEVSNDEP 367

Query: 365 LKSYIPHWKKLSCPEDLWCIKRLREGYADNSIGDWTKSPPAFSIFVDPREF 408
           LKS IPHWKKLSCPEDLWCIKRL EGY +NS+GDWTKSPP+FSIFVDPREF
Sbjct: 368 LKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 418

BLAST of Lcy07g005560 vs. NCBI nr
Match: XP_022932904.1 (probable N-acetyltransferase HLS1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 685.6 bits (1768), Expect = 2.5e-193
Identity = 342/411 (83.21%), Postives = 359/411 (87.35%), Query Frame = 0

Query: 5   KKRKKKLEILIREFDPIKDRAGVEDVERRCEVGSTKNKKLTLFTDLLGDPICRVRHSPAF 64
           KKRK  ++ILIREFDPIKD+  VEDVERRCEVGSTKNK  +LFTDLLGDPICRVRHSPAF
Sbjct: 9   KKRKNNIQILIREFDPIKDKPSVEDVERRCEVGSTKNKNFSLFTDLLGDPICRVRHSPAF 68

Query: 65  LMLVAEIDADKEIVGMIRGCIKTVSCCSKLPRNGGIKGSALIQHLPVYTKLAYILGLRVS 124
           LMLVAEI A  EIVGMIRGCIKTV+CCSK  RN GI  S L Q  PVYTKLAYILGLRVS
Sbjct: 69  LMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNAGINASDLPQLAPVYTKLAYILGLRVS 128

Query: 125 PAHRRLGIGMKLVRRMEEWFNENKAEYSYIATESDNVASIKLFIEICGYSKFRTPSILVN 184
           P H RLGIG+ LVRRMEEWF E KAEYSYIATE DNVASIKLF   CGYSKFRTPSILVN
Sbjct: 129 PDH-RLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYSKFRTPSILVN 188

Query: 185 PVFAHRLPLSDRVTILQLGLADAETLYRRRFATAEFFPLDIDAVLCNRLSLGTFLAVPRG 244
           PVFAHRL LSD+VTILQL L  AETLYR RFATAEFFP DIDAVL NRLSLGTFLAVPRG
Sbjct: 189 PVFAHRLRLSDQVTILQLELTVAETLYRCRFATAEFFPRDIDAVLSNRLSLGTFLAVPRG 248

Query: 245 S----SWVGSDR----LPESWAILSAWNCKDMYALEVRGASATKRAVAKISRMIDRGLPW 304
           S     WV SDR    LPESWA+LSAWNCKD+YALEVRG S  KRA+AK+SR+IDRGLPW
Sbjct: 249 SFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKLSRVIDRGLPW 308

Query: 305 LRVPSVPEVFSPFGVLFLYGVGGEGPHAGKLMKALCNHAHNLAKERGCGVVATEVSNDEP 364
           LR+PSVPEVF PFGVLFLYGVGGEGP AGKLMKALC+HAHNLAKERGCGVVATEVSNDEP
Sbjct: 309 LRLPSVPEVFKPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVATEVSNDEP 368

Query: 365 LKSYIPHWKKLSCPEDLWCIKRLREGYADNSIGDWTKSPPAFSIFVDPREF 408
           LKS IPHWKKLSCPEDLWCIKRL EGY +NS+GDWTKSPP+FSIFVDPREF
Sbjct: 369 LKSDIPHWKKLSCPEDLWCIKRLGEGYTNNSLGDWTKSPPSFSIFVDPREF 418

BLAST of Lcy07g005560 vs. NCBI nr
Match: XP_022986693.1 (probable N-acetyltransferase HLS1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 681.8 bits (1758), Expect = 3.6e-192
Identity = 339/411 (82.48%), Postives = 355/411 (86.37%), Query Frame = 0

Query: 5   KKRKKKLEILIREFDPIKDRAGVEDVERRCEVGSTKNKKLTLFTDLLGDPICRVRHSPAF 64
           KKRK  ++ILIREFDP KDR  VEDVERRCEVGSTKNK  +LFTDLLGDPICRVRHSPAF
Sbjct: 8   KKRKNNIQILIREFDPTKDRPAVEDVERRCEVGSTKNKNFSLFTDLLGDPICRVRHSPAF 67

Query: 65  LMLVAEIDADKEIVGMIRGCIKTVSCCSKLPRNGGIKGSALIQHLPVYTKLAYILGLRVS 124
           LMLVAEI A  EIVGMIRGCIKTV+CCSK  RN GI  S L    PVYTKLAYILGLRVS
Sbjct: 68  LMLVAEIAAHNEIVGMIRGCIKTVTCCSKSTRNSGINASDLPHLAPVYTKLAYILGLRVS 127

Query: 125 PAHRRLGIGMKLVRRMEEWFNENKAEYSYIATESDNVASIKLFIEICGYSKFRTPSILVN 184
           P HRRLGIG+ LVRRMEEWF E KAEYSYIATE DNVASIKLF   CGY KFRTPSILVN
Sbjct: 128 PDHRRLGIGLNLVRRMEEWFREKKAEYSYIATEIDNVASIKLFTHKCGYFKFRTPSILVN 187

Query: 185 PVFAHRLPLSDRVTILQLGLADAETLYRRRFATAEFFPLDIDAVLCNRLSLGTFLAVPRG 244
           PVFAHRL LSD+VTIL+L L  AETLYR RFA AEFFP DIDAVL NRLSLGTFLAVPRG
Sbjct: 188 PVFAHRLRLSDQVTILRLELTVAETLYRCRFAAAEFFPRDIDAVLSNRLSLGTFLAVPRG 247

Query: 245 S----SWVGSDR----LPESWAILSAWNCKDMYALEVRGASATKRAVAKISRMIDRGLPW 304
           S     WV SDR    LPESWA+LSAWNCKD+YALEVRG S  KRA+AK SR+IDRGLPW
Sbjct: 248 SFTDGLWVESDRFLSCLPESWAVLSAWNCKDVYALEVRGVSVVKRAMAKFSRVIDRGLPW 307

Query: 305 LRVPSVPEVFSPFGVLFLYGVGGEGPHAGKLMKALCNHAHNLAKERGCGVVATEVSNDEP 364
           LR+PSVPEVF+PFGVLFLYGVGGEGP AGKLMKALC+HAHNLAKERGCGVVATEVSNDEP
Sbjct: 308 LRLPSVPEVFTPFGVLFLYGVGGEGPLAGKLMKALCHHAHNLAKERGCGVVATEVSNDEP 367

Query: 365 LKSYIPHWKKLSCPEDLWCIKRLREGYADNSIGDWTKSPPAFSIFVDPREF 408
           LKS IPHWKKLSCPEDLWCIKRL E Y DNS+GDWTKSPP+FSIFVDPREF
Sbjct: 368 LKSDIPHWKKLSCPEDLWCIKRLGECYTDNSLGDWTKSPPSFSIFVDPREF 418

BLAST of Lcy07g005560 vs. TAIR 10
Match: AT4G37580.1 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 545.8 bits (1405), Expect = 2.9e-155
Identity = 264/406 (65.02%), Postives = 320/406 (78.82%), Query Frame = 0

Query: 14  LIREFDPIKDRAGVEDVERRCEVGSTKNKKLTLFTDLLGDPICRVRHSPAFLMLVAEIDA 73
           ++RE+DP +D  GVEDVERRCEVG   + KL+LFTDLLGDPICR+RHSP++LMLVAE+  
Sbjct: 3   VVREYDPTRDLVGVEDVERRCEVG--PSGKLSLFTDLLGDPICRIRHSPSYLMLVAEMGT 62

Query: 74  D-KEIVGMIRGCIKTVSCCSKLPRNGGIKGSALIQHLPVYTKLAYILGLRVSPAHRRLGI 133
           + KEIVGMIRGCIKTV+C  KL  N   +   +    P+YTKLAY+LGLRVSP HRR GI
Sbjct: 63  EKKEIVGMIRGCIKTVTCGQKLDLNHKSQNDVV---KPLYTKLAYVLGLRVSPFHRRQGI 122

Query: 134 GMKLVRRMEEWFNENKAEYSYIATESDNVASIKLFIEICGYSKFRTPSILVNPVFAHRLP 193
           G KLV+ MEEWF +N AEYSYIATE+DN AS+ LF   CGYS+FRTPSILVNPV+AHR+ 
Sbjct: 123 GFKLVKMMEEWFRQNGAEYSYIATENDNQASVNLFTGKCGYSEFRTPSILVNPVYAHRVN 182

Query: 194 LSDRVTILQLGLADAETLYRRRFATAEFFPLDIDAVLCNRLSLGTFLAVPRGS------- 253
           +S RVT+++L   DAETLYR RF+T EFFP DID+VL N+LSLGTF+AVPRGS       
Sbjct: 183 VSRRVTVIKLEPVDAETLYRIRFSTTEFFPRDIDSVLNNKLSLGTFVAVPRGSCYGSGSG 242

Query: 254 SWVGSDRL----PESWAILSAWNCKDMYALEVRGASATKRAVAKISRMIDRGLPWLRVPS 313
           SW GS +     PESWA+LS WNCKD + LEVRGAS  +R VAK +R++D+ LP+L++PS
Sbjct: 243 SWPGSAKFLEYPPESWAVLSVWNCKDSFLLEVRGASRLRRVVAKTTRVVDKTLPFLKLPS 302

Query: 314 VPEVFSPFGVLFLYGVGGEGPHAGKLMKALCNHAHNLAKERGCGVVATEVSNDEPLKSYI 373
           +P VF PFG+ F+YG+GGEGP A K++K+LC HAHNLAK  GCGVVA EV+ ++PL+  I
Sbjct: 303 IPSVFEPFGLHFMYGIGGEGPRAVKMVKSLCAHAHNLAKAGGCGVVAAEVAGEDPLRRGI 362

Query: 374 PHWKKLSCPEDLWCIKRLREGYADNSIGDWTKSPPAFSIFVDPREF 408
           PHWK LSC EDLWCIKRL + Y+D  +GDWTKSPP  SIFVDPREF
Sbjct: 363 PHWKVLSCDEDLWCIKRLGDDYSDGVVGDWTKSPPGVSIFVDPREF 403

BLAST of Lcy07g005560 vs. TAIR 10
Match: AT2G23060.1 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 543.1 bits (1398), Expect = 1.9e-154
Identity = 265/409 (64.79%), Postives = 320/409 (78.24%), Query Frame = 0

Query: 15  IREFDPIKDRAGVEDVERRCEVGSTKNKKLTLFTDLLGDPICRVRHSPAFLMLVAEI--D 74
           +RE+DP KD A VEDVERRCEVG     KL+LFTDLLGDPICRVRHSP++LMLVAEI   
Sbjct: 7   VREYDPSKDLATVEDVERRCEVGPA--GKLSLFTDLLGDPICRVRHSPSYLMLVAEIGPK 66

Query: 75  ADKEIVGMIRGCIKTVSC---CSKLPRNGGIKGSALIQHLPVYTKLAYILGLRVSPAHRR 134
             KE+VGMIRGCIKTV+C     +L        + ++   P+YTKLAYILGLRVSP HRR
Sbjct: 67  EKKELVGMIRGCIKTVTCGITTKRLDLTHNKSQNDVVITKPLYTKLAYILGLRVSPTHRR 126

Query: 135 LGIGMKLVRRMEEWFNENKAEYSYIATESDNVASIKLFIEICGYSKFRTPSILVNPVFAH 194
            GIG KLV+ ME+WF++N AEYSY ATE+DN AS+ LF   CGY++FRTPSILVNPV+AH
Sbjct: 127 QGIGFKLVKAMEDWFSQNGAEYSYFATENDNHASVNLFTGKCGYAEFRTPSILVNPVYAH 186

Query: 195 RLPLSDRVTILQLGLADAETLYRRRFATAEFFPLDIDAVLCNRLSLGTFLAVPRGS---- 254
           R+ +S RVT+++L  +DAE LYR RF+T EFFP DID+VL N+LSLGTF+AVPRGS    
Sbjct: 187 RVNISRRVTVIKLEPSDAELLYRLRFSTTEFFPRDIDSVLNNKLSLGTFVAVPRGSCYGS 246

Query: 255 ---SWVGSDRL----PESWAILSAWNCKDMYALEVRGASATKRAVAKISRMIDRGLPWLR 314
              SW GS +     P+SWA+LS WNCKD + LEVRGAS  +R V+K +RM+D+ LP+L+
Sbjct: 247 GSRSWPGSAKFLEYPPDSWAVLSVWNCKDSFRLEVRGASRLRRVVSKATRMVDKTLPFLK 306

Query: 315 VPSVPEVFSPFGVLFLYGVGGEGPHAGKLMKALCNHAHNLAKERGCGVVATEVSNDEPLK 374
           +PS+P VF PFG+ F+YG+GGEGP A K++KALC+HAHNLAKE GCGVVA EV+ +EPL+
Sbjct: 307 IPSIPAVFRPFGLHFMYGIGGEGPRAEKMVKALCDHAHNLAKEGGCGVVAAEVAGEEPLR 366

Query: 375 SYIPHWKKLSCPEDLWCIKRLREGYADNSIGDWTKSPPAFSIFVDPREF 408
             IPHWK LSC EDLWCIKRL E Y+D S+GDWTKSPP  SIFVDPREF
Sbjct: 367 RGIPHWKVLSCAEDLWCIKRLGEDYSDGSVGDWTKSPPGDSIFVDPREF 413

BLAST of Lcy07g005560 vs. TAIR 10
Match: AT2G23060.2 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 468.0 bits (1203), Expect = 7.7e-132
Identity = 227/358 (63.41%), Postives = 277/358 (77.37%), Query Frame = 0

Query: 66  MLVAEI--DADKEIVGMIRGCIKTVSC---CSKLPRNGGIKGSALIQHLPVYTKLAYILG 125
           MLVAEI     KE+VGMIRGCIKTV+C     +L        + ++   P+YTKLAYILG
Sbjct: 1   MLVAEIGPKEKKELVGMIRGCIKTVTCGITTKRLDLTHNKSQNDVVITKPLYTKLAYILG 60

Query: 126 LRVSPAHRRLGIGMKLVRRMEEWFNENKAEYSYIATESDNVASIKLFIEICGYSKFRTPS 185
           LRVSP HRR GIG KLV+ ME+WF++N AEYSY ATE+DN AS+ LF   CGY++FRTPS
Sbjct: 61  LRVSPTHRRQGIGFKLVKAMEDWFSQNGAEYSYFATENDNHASVNLFTGKCGYAEFRTPS 120

Query: 186 ILVNPVFAHRLPLSDRVTILQLGLADAETLYRRRFATAEFFPLDIDAVLCNRLSLGTFLA 245
           ILVNPV+AHR+ +S RVT+++L  +DAE LYR RF+T EFFP DID+VL N+LSLGTF+A
Sbjct: 121 ILVNPVYAHRVNISRRVTVIKLEPSDAELLYRLRFSTTEFFPRDIDSVLNNKLSLGTFVA 180

Query: 246 VPRGS-------SWVGSDRL----PESWAILSAWNCKDMYALEVRGASATKRAVAKISRM 305
           VPRGS       SW GS +     P+SWA+LS WNCKD + LEVRGAS  +R V+K +RM
Sbjct: 181 VPRGSCYGSGSRSWPGSAKFLEYPPDSWAVLSVWNCKDSFRLEVRGASRLRRVVSKATRM 240

Query: 306 IDRGLPWLRVPSVPEVFSPFGVLFLYGVGGEGPHAGKLMKALCNHAHNLAKERGCGVVAT 365
           +D+ LP+L++PS+P VF PFG+ F+YG+GGEGP A K++KALC+HAHNLAKE GCGVVA 
Sbjct: 241 VDKTLPFLKIPSIPAVFRPFGLHFMYGIGGEGPRAEKMVKALCDHAHNLAKEGGCGVVAA 300

Query: 366 EVSNDEPLKSYIPHWKKLSCPEDLWCIKRLREGYADNSIGDWTKSPPAFSIFVDPREF 408
           EV+ +EPL+  IPHWK LSC EDLWCIKRL E Y+D S+GDWTKSPP  SIFVDPREF
Sbjct: 301 EVAGEEPLRRGIPHWKVLSCAEDLWCIKRLGEDYSDGSVGDWTKSPPGDSIFVDPREF 358

BLAST of Lcy07g005560 vs. TAIR 10
Match: AT5G67430.1 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 438.7 bits (1127), Expect = 5.0e-123
Identity = 222/397 (55.92%), Postives = 288/397 (72.54%), Query Frame = 0

Query: 13  ILIREFDPIKDRAGVEDVERRCEVGSTKNKKLTLFTDLLGDPICRVRHSPAFLMLVAEID 72
           +++RE+DP +D   VE++E  CEVGS       L  DL+GDP+ R+R SP+F MLVAEI 
Sbjct: 8   VVVREYDPKRDLTSVEELEESCEVGS-------LLVDLMGDPLARIRQSPSFHMLVAEI- 67

Query: 73  ADKEIVGMIRGCIKTVSCCSKLPRNGGIKGSALIQHLPVYTKLAYILGLRVSPAHRRLGI 132
              EIVGMIRG IK V+      R        +       TKLA++ GLRVSP +RR+GI
Sbjct: 68  -GNEIVGMIRGTIKMVTRGVNALRQADDVSPEINT-----TKLAFVSGLRVSPFYRRMGI 127

Query: 133 GMKLVRRMEEWFNENKAEYSYIATESDNVASIKLFIEICGYSKFRTPSILVNPVFAHRLP 192
           G+KLV+R+EEWF  N A YSY+ TE+DN+AS+KLF E  GYSKFRTP+ LVNPVF HR+ 
Sbjct: 128 GLKLVQRLEEWFLRNDAVYSYVQTENDNIASVKLFTEKSGYSKFRTPTFLVNPVFNHRVT 187

Query: 193 LSDRVTILQLGLADAETLYRRRFATAEFFPLDIDAVLCNRLSLGTFLAVPRGSSWVGSDR 252
           +S RV I++L  +DAE+LYR RF+T EFFP DI+++L N+LSLGT+LAVPRG   V S  
Sbjct: 188 VSRRVKIIKLAPSDAESLYRNRFSTTEFFPSDINSILTNKLSLGTYLAVPRGGDNV-SGS 247

Query: 253 LPE---SWAILSAWNCKDMYALEVRGASATKRAVAKISRMIDRGLPWLRVPSVPEVFSPF 312
           LP+   SWA++S WN KD+Y L+V+GAS  KR +AK +R+ D   P+L++PS P +F  F
Sbjct: 248 LPDQTGSWAVISIWNSKDVYRLQVKGASRLKRMLAKSTRVFDGAFPFLKIPSFPNLFKSF 307

Query: 313 GVLFLYGVGGEGPHAGKLMKALCNHAHNLAKERGCGVVATEVSNDEPLKSYIPHWKKLSC 372
            + F+YG+GGEGP A ++++ALC+HAHNLA++ GC VVA EV++ EPL+  IPHWK LS 
Sbjct: 308 AMHFMYGIGGEGPRAAEMVEALCSHAHNLARKSGCAVVAAEVASCEPLRVGIPHWKVLS- 367

Query: 373 PEDLWCIKRLREGYADNSIGDWTKSPPAFSIFVDPRE 407
           PEDLWC+KRLR  Y D+ + DWTKSPP  SIFVDPRE
Sbjct: 368 PEDLWCLKRLR--YDDDGV-DWTKSPPGLSIFVDPRE 385

BLAST of Lcy07g005560 vs. TAIR 10
Match: AT2G30090.1 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 302.4 bits (773), Expect = 5.6e-82
Identity = 173/404 (42.82%), Postives = 240/404 (59.41%), Query Frame = 0

Query: 12  EILIREFDPIKDRAGVEDVERRCEVGSTKNKKLTLFTDLLGDPICRVRHSPAFLMLVAEI 71
           E++IR +D  +DR  +  +E+ CE+G   + +  LFTD LGDPICR+R+SP F+MLVA +
Sbjct: 12  EVVIRCYDDRRDRIQMGRMEKSCEIG--HDHQTLLFTDTLGDPICRIRNSPFFIMLVAGV 71

Query: 72  DADKEIVGMIRGCIKTVSCCSKLPRNGGIKGSALIQHLPVYTKLAYILGLRVSPAHRRLG 131
               ++VG I+G +K V    K  R G                  Y+LGLRV P++RR G
Sbjct: 72  --GNKLVGSIQGSVKPVEFHDKSVRVG------------------YVLGLRVVPSYRRRG 131

Query: 132 IGMKLVRRMEEWFNENKAEYSYIATESDNVASIKLFIEICGYSKFRTPSILVNPVFAHR- 191
           IG  LVR++EEWF  + A+Y+Y+ATE DN AS  LFI   GY  FR P+ILVNPV   R 
Sbjct: 132 IGSILVRKLEEWFESHNADYAYMATEKDNEASHGLFIGRLGYVVFRNPAILVNPVNPGRG 191

Query: 192 LPLSDRVTILQLGLADAETLYRRRF-ATAEFFPLDIDAVLCNRLSLGTFLAVPRGSSWVG 251
           L L   + I +L + +AE+LYRR   AT EFFP DI+ +L N+LS+GT++A      +  
Sbjct: 192 LKLPSDIGIRKLKVKEAESLYRRNVAATTEFFPDDINKILRNKLSIGTWVA------YYN 251

Query: 252 SDRLPESWAILSAWNCKDMYALEVRGASATKRAVAKISRMIDRGLPWLRVPSVPEVFSPF 311
           +     SWA+LS W+   ++ L +  A  +   + K+S++    L  L +  +P++F+PF
Sbjct: 252 NVDNTRSWAMLSVWDSSKVFKLRIERAPLSYLLLTKVSKLFGNFLSLLGLTVLPDLFTPF 311

Query: 312 GVLFLYGVGGEGPHAGKLMKALCNHAHNLAKER---GCGVVATEV----SNDEPLKSYIP 371
           G  FLYGV  EGPH GKL++ALC H HN+A       C VV  EV    + D+ L+  IP
Sbjct: 312 GFYFLYGVHSEGPHCGKLVRALCEHVHNMAALNDGCACKVVVVEVDKGSNGDDSLQRCIP 371

Query: 372 HWKKLSCPEDLWCIKRLREGYADNSIGDWTKSPPAFSIFVDPRE 407
           HWK LSC +D+WCIK L+     N      +S    S+FVDPRE
Sbjct: 372 HWKMLSCDDDMWCIKPLK--CEKNKFDLSERSKSRSSLFVDPRE 385

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q423814.1e-15465.02Probable N-acetyltransferase HLS1 OS=Arabidopsis thaliana OX=3702 GN=HLS1 PE=1 S... [more]
O648152.7e-15364.79Probable N-acetyltransferase HLS1-like OS=Arabidopsis thaliana OX=3702 GN=At2g23... [more]
Match NameE-valueIdentityDescription
A0A6J1F3291.3e-19583.45probable N-acetyltransferase HLS1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1EYB01.2e-19383.21probable N-acetyltransferase HLS1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1JHA41.8e-19282.48probable N-acetyltransferase HLS1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1JGS41.7e-19082.24probable N-acetyltransferase HLS1 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A5C7HQF52.0e-16471.46N-acetyltransferase domain-containing protein OS=Acer yangbiense OX=1000413 GN=E... [more]
Match NameE-valueIdentityDescription
XP_023512928.17.1e-19683.70probable N-acetyltransferase HLS1 [Cucurbita pepo subsp. pepo] >XP_023522343.1 p... [more]
XP_022932903.12.7e-19583.45probable N-acetyltransferase HLS1 isoform X1 [Cucurbita moschata] >KAG7011045.1 ... [more]
KAG6571244.13.0e-19482.97putative N-acetyltransferase HLS1-like protein, partial [Cucurbita argyrosperma ... [more]
XP_022932904.12.5e-19383.21probable N-acetyltransferase HLS1 isoform X2 [Cucurbita moschata][more]
XP_022986693.13.6e-19282.48probable N-acetyltransferase HLS1 isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G37580.12.9e-15565.02Acyl-CoA N-acyltransferases (NAT) superfamily protein [more]
AT2G23060.11.9e-15464.79Acyl-CoA N-acyltransferases (NAT) superfamily protein [more]
AT2G23060.27.7e-13263.41Acyl-CoA N-acyltransferases (NAT) superfamily protein [more]
AT5G67430.15.0e-12355.92Acyl-CoA N-acyltransferases (NAT) superfamily protein [more]
AT2G30090.15.6e-8242.82Acyl-CoA N-acyltransferases (NAT) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.630.30coord: 8..179
e-value: 1.9E-14
score: 55.8
NoneNo IPR availablePANTHERPTHR43072N-ACETYLTRANSFERASEcoord: 9..407
NoneNo IPR availablePANTHERPTHR43072:SF42N-ACETYLTRANSFERASE HLS1-LIKE-RELATEDcoord: 9..407
NoneNo IPR availableCDDcd04301NAT_SFcoord: 96..151
e-value: 3.32449E-7
score: 45.3445
IPR000182GNAT domainPFAMPF00583Acetyltransf_1coord: 65..173
e-value: 9.9E-16
score: 58.0
IPR000182GNAT domainPROSITEPS51186GNATcoord: 13..194
score: 14.158922
IPR016181Acyl-CoA N-acyltransferaseSUPERFAMILY55729Acyl-CoA N-acyltransferases (Nat)coord: 61..175

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy07g005560.1Lcy07g005560.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0008080 N-acetyltransferase activity