Lcy06g022400 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy06g022400
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	aspartic proteinase PCS1
Location	Chr06: 47243970 .. 47245930 (-)
RNA-Seq Expression	Lcy06g022400
Synteny	Lcy06g022400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGCTTCTCCACCGAATAGCGAACACCCTCCAGAGATCTCAGTGTGAATTTCTTTTTTCCTTTTTTTTTTTTTTTGGAATTTATTGGATTTCTCTTTTTAATAATCGGGATTTATATTTTTGGATGTTCGCAGGGGAGAAGTTTCTCAAAGGATTTCAAAGATTTCAGAACACTCTGTTCTCATAAACCAAAACAGAGCCAACGAACTTCCTCAGAGTTCTTCACTCTGCTTCTTCCAACTGCCCCAACTGTTCGCCAATTCCAATTTTCCAGGAAAGAAAAAGAAAAAAAAAAATGAGGGATTTCTGTATAAATTTCAATTCCTCAAACAACCCATTTCTCAAATCTTGTTTCCTTTTCTTCTTCAGTATGATTTTGTTCTTTCAAAATCCCAACCTCTGTTCTTCATTAAACCCAGCCCTCGTTTTGCCTCTCAAAACCCAAGTAATTCCGCCGGAATCCGTCCGGCGATCTCCCGACAAGCTCCCCTTCCGGCACAACGTCAGCCTCACCGTCTCTCTCACCGTCGGAACGCCGCCGCAGAATGTCACAATGGTCCTCGACACCGGCAGCGAACTCTCATGGCTCCACTGCAACAGATCACAAAATTCCTCTTCTTCATCTTCAACATTCAACCCGGCTCGATCCTCCTCGTACACTCCGATTCCTTGTTCCTCCGCCACCTGCACAGACCAAACTCGGGATTTTCCGATTCCGGCATCTTGTGATTCCAACCAGCTCTGCCACGCCACTCTGTCTTACGCCGACGCCTCTTCTTCAGAAGGAAATCTCGCCACCGATACGTTTCTCATCGGAAATTCGGGAATTTCGAATGTGGTTTTCGGTTGTATGGATTCAATTTTCAGCTCCAACAACGAAGAGGACTCCAAGAACACCGGATTAATGGGTATGAATCGTGGATCTTTATCTTTTGTTTCTCAAATGGGTTTCCCCAAATTTTCATATTGCATATCGGAATATGATTTTTCCGGTTTGTTACTGCTCGGAGATGCTAATTTCTCATGGTTGGCTCCGTTGAATTACACTCCATTGATCGAAATGTCGACGCCGTTACCATATTTCGATCGGGTCGCTTACACGGTTCAATTGGAAGGAATCAAAGTTTCGAACCAGTTACTCCCGATACCGGAATCCGTTTTCGAACCGGACCACACCGGCGCCGGTCAGACCATGGTCGACTCCGGCACCCAGTTCACATTCCTTCTCGGACCGGCTTACACCGCGCTCCGAGACGAGTTTCTAAACCAAACCGCCGGTTCGATCCGGGTTTTCGAGGATCCGAATTTCGTTTTCCAAGGAGCCATGGATCTCTGCTACCGGGTTCCGCTGAATCAAACCCGGCTCCCGCCGCTGCCGGCGGTGACGCTGGTATTCCGCGGCGCTGAAATGACGGTGACCGGTGACCGGATTCTGTACCGGGTGCCCGGAGAAATCAGAGGGAACGATTCGATCCATTGCTTTACATTCGGAAATTCGGATCTTCTCGGCGTTGAAGCGTACGTGATCGGGCATCTTCATCAACAAAACGTGTGGATGGAATTCGATCTGAAAAAATCGCGGATCGGGTTGGCGGAGATCCGGTGCGATTTAGCGGGTCAGAAATTGGGCATGGGCCTGTAAATGACCCGGGCCCATTTGATAGAAGCGTTTTAACTATGGGGTGAACTTAGCACGTGATATTGATTAAAATCTTGCGGGTTTTGGTGTGTAGTCGTAGGTCCACGCTATTATGGTGGTCCTTCAGCTTTTAATACCGTCCGTATGTGGGTCCCCTGCCATCTGTAATATTATAAAAAAAAAACAAAAAGCAAAAGTGAGTTTGATCTGACGGGTGTAGTGAGTTTAAGTGAGCTGCGATGTCGCCACGTGTAAGGAGAGTTTTTTCTCTTTTTTTTTTTTTGCTTTTTTTTTCCTCCCTACCTGTACATTTTAAATATTCAT

mRNA sequence

TGCTTCTCCACCGAATAGCGAACACCCTCCAGAGATCTCAGTGGGAGAAGTTTCTCAAAGGATTTCAAAGATTTCAGAACACTCTGTTCTCATAAACCAAAACAGAGCCAACGAACTTCCTCAGAGTTCTTCACTCTGCTTCTTCCAACTGCCCCAACTGTTCGCCAATTCCAATTTTCCAGGAAAGAAAAAGAAAAAAAAAAATGAGGGATTTCTGTATAAATTTCAATTCCTCAAACAACCCATTTCTCAAATCTTGTTTCCTTTTCTTCTTCAGTATGATTTTGTTCTTTCAAAATCCCAACCTCTGTTCTTCATTAAACCCAGCCCTCGTTTTGCCTCTCAAAACCCAAGTAATTCCGCCGGAATCCGTCCGGCGATCTCCCGACAAGCTCCCCTTCCGGCACAACGTCAGCCTCACCGTCTCTCTCACCGTCGGAACGCCGCCGCAGAATGTCACAATGGTCCTCGACACCGGCAGCGAACTCTCATGGCTCCACTGCAACAGATCACAAAATTCCTCTTCTTCATCTTCAACATTCAACCCGGCTCGATCCTCCTCGTACACTCCGATTCCTTGTTCCTCCGCCACCTGCACAGACCAAACTCGGGATTTTCCGATTCCGGCATCTTGTGATTCCAACCAGCTCTGCCACGCCACTCTGTCTTACGCCGACGCCTCTTCTTCAGAAGGAAATCTCGCCACCGATACGTTTCTCATCGGAAATTCGGGAATTTCGAATGTGGTTTTCGGTTGTATGGATTCAATTTTCAGCTCCAACAACGAAGAGGACTCCAAGAACACCGGATTAATGGGTATGAATCGTGGATCTTTATCTTTTGTTTCTCAAATGGGTTTCCCCAAATTTTCATATTGCATATCGGAATATGATTTTTCCGGTTTGTTACTGCTCGGAGATGCTAATTTCTCATGGTTGGCTCCGTTGAATTACACTCCATTGATCGAAATGTCGACGCCGTTACCATATTTCGATCGGGTCGCTTACACGGTTCAATTGGAAGGAATCAAAGTTTCGAACCAGTTACTCCCGATACCGGAATCCGTTTTCGAACCGGACCACACCGGCGCCGGTCAGACCATGGTCGACTCCGGCACCCAGTTCACATTCCTTCTCGGACCGGCTTACACCGCGCTCCGAGACGAGTTTCTAAACCAAACCGCCGGTTCGATCCGGGTTTTCGAGGATCCGAATTTCGTTTTCCAAGGAGCCATGGATCTCTGCTACCGGGTTCCGCTGAATCAAACCCGGCTCCCGCCGCTGCCGGCGGTGACGCTGGTATTCCGCGGCGCTGAAATGACGGTGACCGGTGACCGGATTCTGTACCGGGTGCCCGGAGAAATCAGAGGGAACGATTCGATCCATTGCTTTACATTCGGAAATTCGGATCTTCTCGGCGTTGAAGCGTACGTGATCGGGCATCTTCATCAACAAAACGTGTGGATGGAATTCGATCTGAAAAAATCGCGGATCGGGTTGGCGGAGATCCGGTGCGATTTAGCGGGTCAGAAATTGGGCATGGGCCTGTAAATGACCCGGGCCCATTTGATAGAAGCGTTTTAACTATGGGGTGAACTTAGCACGTGATATTGATTAAAATCTTGCGGGTTTTGGTGTGTAGTCGTAGGTCCACGCTATTATGGTGGTCCTTCAGCTTTTAATACCGTCCGTATGTGGGTCCCCTGCCATCTGTAATATTATAAAAAAAAAACAAAAAGCAAAAGTGAGTTTGATCTGACGGGTGTAGTGAGTTTAAGTGAGCTGCGATGTCGCCACGTGTAAGGAGAGTTTTTTCTCTTTTTTTTTTTTTGCTTTTTTTTTCCTCCCTACCTGTACATTTTAAATATTCAT

Coding sequence (CDS)

ATGAGGGATTTCTGTATAAATTTCAATTCCTCAAACAACCCATTTCTCAAATCTTGTTTCCTTTTCTTCTTCAGTATGATTTTGTTCTTTCAAAATCCCAACCTCTGTTCTTCATTAAACCCAGCCCTCGTTTTGCCTCTCAAAACCCAAGTAATTCCGCCGGAATCCGTCCGGCGATCTCCCGACAAGCTCCCCTTCCGGCACAACGTCAGCCTCACCGTCTCTCTCACCGTCGGAACGCCGCCGCAGAATGTCACAATGGTCCTCGACACCGGCAGCGAACTCTCATGGCTCCACTGCAACAGATCACAAAATTCCTCTTCTTCATCTTCAACATTCAACCCGGCTCGATCCTCCTCGTACACTCCGATTCCTTGTTCCTCCGCCACCTGCACAGACCAAACTCGGGATTTTCCGATTCCGGCATCTTGTGATTCCAACCAGCTCTGCCACGCCACTCTGTCTTACGCCGACGCCTCTTCTTCAGAAGGAAATCTCGCCACCGATACGTTTCTCATCGGAAATTCGGGAATTTCGAATGTGGTTTTCGGTTGTATGGATTCAATTTTCAGCTCCAACAACGAAGAGGACTCCAAGAACACCGGATTAATGGGTATGAATCGTGGATCTTTATCTTTTGTTTCTCAAATGGGTTTCCCCAAATTTTCATATTGCATATCGGAATATGATTTTTCCGGTTTGTTACTGCTCGGAGATGCTAATTTCTCATGGTTGGCTCCGTTGAATTACACTCCATTGATCGAAATGTCGACGCCGTTACCATATTTCGATCGGGTCGCTTACACGGTTCAATTGGAAGGAATCAAAGTTTCGAACCAGTTACTCCCGATACCGGAATCCGTTTTCGAACCGGACCACACCGGCGCCGGTCAGACCATGGTCGACTCCGGCACCCAGTTCACATTCCTTCTCGGACCGGCTTACACCGCGCTCCGAGACGAGTTTCTAAACCAAACCGCCGGTTCGATCCGGGTTTTCGAGGATCCGAATTTCGTTTTCCAAGGAGCCATGGATCTCTGCTACCGGGTTCCGCTGAATCAAACCCGGCTCCCGCCGCTGCCGGCGGTGACGCTGGTATTCCGCGGCGCTGAAATGACGGTGACCGGTGACCGGATTCTGTACCGGGTGCCCGGAGAAATCAGAGGGAACGATTCGATCCATTGCTTTACATTCGGAAATTCGGATCTTCTCGGCGTTGAAGCGTACGTGATCGGGCATCTTCATCAACAAAACGTGTGGATGGAATTCGATCTGAAAAAATCGCGGATCGGGTTGGCGGAGATCCGGTGCGATTTAGCGGGTCAGAAATTGGGCATGGGCCTGTAA

Protein sequence

MRDFCINFNSSNNPFLKSCFLFFFSMILFFQNPNLCSSLNPALVLPLKTQVIPPESVRRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVLDTGSELSWLHCNRSQNSSSSSSTFNPARSSSYTPIPCSSATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLATDTFLIGNSGISNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSNQLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVFEDPNFVFQGAMDLCYRVPLNQTRLPPLPAVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL

Homology

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BLAST of Lcy06g022400 vs. ExPASy Swiss-Prot
Match: Q9LZL3 (Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 3.1e-163
Identity = 288/416 (69.23%), Postives = 342/416 (82.21%), Query Frame = 0


Query: 37  SSLNPALVLPLKTQVIPPESVRRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVLDTGSELS 96
           SS +  LVLPLKT++ P +   R  DKL F HNV+LTV+LTVGTPPQN++MV+DTGSELS
Sbjct: 40  SSSSQTLVLPLKTRITPTD--HRPTDKLHFHHNVTLTVTLTVGTPPQNISMVIDTGSELS 99

Query: 97  WLHCNRSQNSSSSSSTFNPARSSSYTPIPCSSATCTDQTRDFPIPASCDSNQLCHATLSY 156
           WL CNRS N +  ++ F+P RSSSY+PIPCSS TC  +TRDF IPASCDS++LCHATLSY
Sbjct: 100 WLRCNRSSNPNPVNN-FDPTRSSSYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHATLSY 159

Query: 157 ADASSSEGNLATDTFLIGNS-GISNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVS 216
           ADASSSEGNLA + F  GNS   SN++FGCM S+  S+ EED+K TGL+GMNRGSLSF+S
Sbjct: 160 ADASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEEDTKTTGLLGMNRGSLSFIS 219

Query: 217 QMGFPKFSYCIS-EYDFSGLLLLGDANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEG 276
           QMGFPKFSYCIS   DF G LLLGD+NF+WL PLNYTPLI +STPLPYFDRVAYTVQL G
Sbjct: 220 QMGFPKFSYCISGTDDFPGFLLLGDSNFTWLTPLNYTPLIRISTPLPYFDRVAYTVQLTG 279

Query: 277 IKVSNQLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVFE 336
           IKV+ +LLPIP+SV  PDHTGAGQTMVDSGTQFTFLLGP YTALR  FLN+T G + V+E
Sbjct: 280 IKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVYE 339

Query: 337 DPNFVFQGAMDLCYRVPLNQTR---LPPLPAVTLVFRGAEMTVTGDRILYRVPGEIRGND 396
           DP+FVFQG MDLCYR+   + R   L  LP V+LVF GAE+ V+G  +LYRVP    GND
Sbjct: 340 DPDFVFQGTMDLCYRISPVRIRSGILHRLPTVSLVFEGAEIAVSGQPLLYRVPHLTVGND 399

Query: 397 SIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMG 448
           S++CFTFGNSDL+G+EAYVIGH HQQN+W+EFDL++SRIGLA + CD++GQ+LG+G
Sbjct: 400 SVYCFTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVECDVSGQRLGIG 452

[Close]

BLAST of Lcy06g022400 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.8e-38
Identity = 119/370 (32.16%), Postives = 187/370 (50.54%), Query Frame = 0


Query: 74  VSLTVGTPPQNVTMVLDTGSELSWLHCNR-SQNSSSSSSTFNPARSSSYTPIPCSSATCT 133
           ++L++GTP Q  + ++DTGS+L W  C   +Q  + S+  FNP  SSS++ +PCSS  C 
Sbjct: 97  MNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSSQLC- 156

Query: 134 DQTRDFPIPASCDSNQLCHATLSYADASSSEGNLATDTFLIGNSGISNVVFGCMDSIFSS 193
            Q    P   +C SN  C  T  Y D S ++G++ T+T   G+  I N+ FGC       
Sbjct: 157 -QALSSP---TC-SNNFCQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGC-----GE 216

Query: 194 NNE--EDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFS--GLLLLGDANFSWLAPL 253
           NN+        GL+GM RG LS  SQ+   KFSYC++    S    LLLG    S  A  
Sbjct: 217 NNQGFGQGNGAGLVGMGRGPLSLPSQLDVTKFSYCMTPIGSSTPSNLLLGSLANSVTAGS 276

Query: 254 NYTPLIEMSTPLPYFDRVAYTVQLEGIKVSNQLLPIPESVFE-PDHTGAGQTMVDSGTQF 313
             T LI+ S+ +P F    Y + L G+ V +  LPI  S F    + G G  ++DSGT  
Sbjct: 277 PNTTLIQ-SSQIPTF----YYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTL 336

Query: 314 TFLLGPAYTALRDEFLNQTAGSIRVFEDPNFVFQGAMDLCYRVPLNQTRLPPLPAVTLVF 373
           T+ +  AY ++R EF++Q   ++ V    +  F    DLC++ P + + L  +P   + F
Sbjct: 337 TYFVNNAYQSVRQEFISQI--NLPVVNGSSSGF----DLCFQTPSDPSNL-QIPTFVMHF 396

Query: 374 RGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKK 433
            G ++ +  +         I  ++ + C   G+S   G+   + G++ QQN+ + +D   
Sbjct: 397 DGGDLELPSENYF------ISPSNGLICLAMGSSS-QGMS--IFGNIQQQNMLVVYDTGN 434

Query: 434 SRIGLAEIRC 438
           S +  A  +C
Sbjct: 457 SVVSFASAQC 434

[Close]

BLAST of Lcy06g022400 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 1.0e-36
Identity = 107/367 (29.16%), Postives = 175/367 (47.68%), Query Frame = 0


Query: 74  VSLTVGTPPQNVTMVLDTGSELSWLHCNR-SQNSSSSSSTFNPARSSSYTPIPCSSATCT 133
           +++ +GTP  + + ++DTGS+L W  C   +Q  S  +  FNP  SSS++ +PC S  C 
Sbjct: 98  MNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSFSTLPCESQYCQ 157

Query: 134 DQTRDFPIPASCDSNQLCHATLSYADASSSEGNLATDTFLIGNSGISNVVFGCMDSIFSS 193
           D      +P+   +N  C  T  Y D S+++G +AT+TF    S + N+ FGC +    +
Sbjct: 158 D------LPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETSSVPNIAFGCGE---DN 217

Query: 194 NNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFS--GLLLLGDANFSWLAPLNY 253
                    GL+GM  G LS  SQ+G  +FSYC++ Y  S    L LG A          
Sbjct: 218 QGFGQGNGAGLIGMGWGPLSLPSQLGVGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPS 277

Query: 254 TPLIEMSTPLPYFDRVAYTVQLEGIKVSNQLLPIPESVFEPDHTGAGQTMVDSGTQFTFL 313
           T LI  S    Y     Y + L+GI V    L IP S F+    G G  ++DSGT  T+L
Sbjct: 278 TTLIHSSLNPTY-----YYITLQGITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYL 337

Query: 314 LGPAYTALRDEFLNQTAGSIRVFEDPNFVFQGAMDLCYRVPLNQTRLPPLPAVTLVFRGA 373
              AY A+   F +Q   ++   ++ +      +  C++ P + + +  +P +++ F G 
Sbjct: 338 PQDAYNAVAQAFTDQI--NLPTVDESS----SGLSTCFQQPSDGSTV-QVPEISMQFDGG 397

Query: 374 EMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSRI 433
            + +    IL      I   + + C   G+S  LG+   + G++ QQ   + +DL+   +
Sbjct: 398 VLNLGEQNIL------ISPAEGVICLAMGSSSQLGIS--IFGNIQQQETQVLYDLQNLAV 435

Query: 434 GLAEIRC 438
                +C
Sbjct: 458 SFVPTQC 435

[Close]

BLAST of Lcy06g022400 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 2.3e-33
Identity = 122/371 (32.88%), Postives = 179/371 (48.25%), Query Frame = 0


Query: 76  LTVGTPPQNVTMVLDTGSELSWLHCNRSQNS-SSSSSTFNPARSSSYTPIPCSSATCTDQ 135
           L VGTP + V MVLDTGS++ WL C   +   S S   F+P +S +Y  IPCSS  C   
Sbjct: 146 LGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRRL 205

Query: 136 TRDFPIPASCDS-NQLCHATLSYADASSSEGNLATDTFLIGNSGISNVVFGCMDSIFSSN 195
                  A C++  + C   +SY D S + G+ +T+T     + +  V  GC       N
Sbjct: 206 D-----SAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGC----GHDN 265

Query: 196 NEEDSKNTGLMGMNRGSLSFVSQMGF---PKFSYCISEYDFS---GLLLLGDANFSWLAP 255
                   GL+G+ +G LSF  Q G     KFSYC+ +   S     ++ G+A  S +A 
Sbjct: 266 EGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIA- 325

Query: 256 LNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSNQLLP-IPESVFEPDHTGAGQTMVDSGTQ 315
             +TPL+      P  D   Y V L GI V    +P +  S+F+ D  G G  ++DSGT 
Sbjct: 326 -RFTPLLSN----PKLD-TFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTS 385

Query: 316 FTFLLGPAYTALRDEFLNQTAGSIRVFEDPNFVFQGAMDLCYRVPLNQTRLPPLPAVTLV 375
            T L+ PAY A+RD F     G+  +   P+F      D C+   L+      +P V L 
Sbjct: 386 VTRLIRPAYIAMRDAF---RVGAKTLKRAPDF---SLFDTCF--DLSNMNEVKVPTVVLH 445

Query: 376 FRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLK 435
           FRGA++++      Y +P +  G     CF F  + + G+   +IG++ QQ   + +DL 
Sbjct: 446 FRGADVSLPATN--YLIPVDTNGK---FCFAFAGT-MGGLS--IIGNIQQQGFRVVYDLA 484

Query: 436 KSRIGLAEIRC 438
            SR+G A   C
Sbjct: 506 SSRVGFAPGGC 484

[Close]

BLAST of Lcy06g022400 vs. ExPASy Swiss-Prot
Match: O04496 (Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 6.7e-33
Identity = 119/378 (31.48%), Postives = 172/378 (45.50%), Query Frame = 0


Query: 68  HNVSLTVSLTVGTPPQNVTMVLDTGSELSWLHCNRSQNSSSSSSTFNPARSSSYTPIPCS 127
           H  +  V   +GTPPQ + MVLDT ++  WL C+     S++S++FN   SS+Y+ + CS
Sbjct: 100 HIGNYVVRAKLGTPPQLMFMVLDTSNDAVWLPCSGCSGCSNASTSFNTNSSSTYSTVSCS 159

Query: 128 SATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLATDTFLIGNSGISNVVFGCMD 187
           +A CT Q R    P+S     +C    SY   SS   +L  DT  +    I N  FGC++
Sbjct: 160 TAQCT-QARGLTCPSSSPQPSVCSFNQSYGGDSSFSASLVQDTLTLAPDVIPNFSFGCIN 219

Query: 188 SIFSSNNEEDSKNTGLMGMNRGSLSFVSQ---MGFPKFSYCISEYD---FSGLLLLGDAN 247
           S  +S N    +  GLMG+ RG +S VSQ   +    FSYC+  +    FSG L LG   
Sbjct: 220 S--ASGNSLPPQ--GLMGLGRGPMSLVSQTTSLYSGVFSYCLPSFRSFYFSGSLKLG--L 279

Query: 248 FSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSNQLLPIPESVFEPDHTGAGQTMV 307
                 + YTPL+  +   P      Y V L G+ V +  +P+       D      T++
Sbjct: 280 LGQPKSIRYTPLLR-NPRRPSL----YYVNLTGVSVGSVQVPVDPVYLTFDANSGAGTII 339

Query: 308 DSGTQFTFLLGPAYTALRDEFLNQTAGSIRVFEDPNFVFQGAMDLCYRVPLNQTRLPPLP 367
           DSGT  T    P Y A+RDEF  Q   S       +F   GA D C+            P
Sbjct: 340 DSGTVITRFAQPVYEAIRDEFRKQVNVS-------SFSTLGAFDTCFSADNENV----AP 399

Query: 368 AVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTF-GNSDLLGVEAYVIGHLHQQNVW 427
            +TL     ++ +  +  L           ++ C +  G          VI +L QQN+ 
Sbjct: 400 KITLHMTSLDLKLPMENTLIH-----SSAGTLTCLSMAGIRQNANAVLNVIANLQQQNLR 449

Query: 428 MEFDLKKSRIGLAEIRCD 439
           + FD+  SRIG+A   C+
Sbjct: 460 ILFDVPNSRIGIAPEPCN 449

[Close]

BLAST of Lcy06g022400 vs. ExPASy TrEMBL
Match: A0A6J1J2F5 (aspartic proteinase PCS1 OS=Cucurbita maxima OX=3661 GN=LOC111482077 PE=3 SV=1)

HSP 1 Score: 842.4 bits (2175), Expect = 8.6e-241
Identity = 419/449 (93.32%), Postives = 433/449 (96.44%), Query Frame = 0


Query: 1   MRDFCINFNSSNNPFLKSCFLFFFSMIL-FFQNPNLCSSLNPALVLPLKTQVIPPESVRR 60
           MRD+CI FNSSN+ FLKS F FF  ++   FQN  LCSSLNPAL+LPLKTQVIPPES+RR
Sbjct: 1   MRDYCIAFNSSNHKFLKSFFPFFLCILFSVFQNLILCSSLNPALLLPLKTQVIPPESIRR 60

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVLDTGSELSWLHCNRSQNSSSSSSTFNPARSS 120
           SPDKLPFRHNVSLTVSLTVGTPPQNVTMV+DTGSELSWLHCNRSQNSSSSSSTFNPA SS
Sbjct: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPAGSS 120

Query: 121 SYTPIPCSSATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLATDTFLIGNSGIS 180
           SYTPIPCSS+TCTDQTRDFPIPASCDSN LCHATLSYADASSSEG LATDTF IGNSGIS
Sbjct: 121 SYTPIPCSSSTCTDQTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGIS 180

Query: 181 NVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD 240
           NVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD
Sbjct: 181 NVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD 240

Query: 241 ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSNQLLPIPESVFEPDHTGAGQT 300
           ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVS++LLPIPESVFEPDHTGAGQT
Sbjct: 241 ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQT 300

Query: 301 MVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVFEDPNFVFQGAMDLCYRVPLNQTRLPP 360
           MVDSGTQFTFLLGPAYTALRDEFLN+TAGSIRVFED NFVFQGAMDLCYRVP+NQTRLPP
Sbjct: 301 MVDSGTQFTFLLGPAYTALRDEFLNRTAGSIRVFEDSNFVFQGAMDLCYRVPMNQTRLPP 360

Query: 361 LPAVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQNV 420
           LP+VTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEA+VIGHLHQQNV
Sbjct: 361 LPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQNV 420

Query: 421 WMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449
           WMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 WMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449

[Close]

BLAST of Lcy06g022400 vs. ExPASy TrEMBL
Match: A0A6J1F9C1 (aspartic proteinase PCS1 OS=Cucurbita moschata OX=3662 GN=LOC111442007 PE=3 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 8.9e-238
Identity = 416/450 (92.44%), Postives = 431/450 (95.78%), Query Frame = 0


Query: 1   MRDFCINFNSSNNPFLKSCFLFFF-SMILFFQNPNLCSSLNPALVLPLKTQVIPPESVRR 60
           MRD+CI FNSSN+ FLKS F FF  ++   FQN  LCSS NPAL+LPLKTQVIPPES+RR
Sbjct: 1   MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 60

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVLDTGSELSWLHCNRSQN-SSSSSSTFNPARS 120
           SPDKLPFRHNVSLTVSLTVGTPPQNVTMV+DTGSELSWLHCNRSQN SSSSSSTFNPA S
Sbjct: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 120

Query: 121 SSYTPIPCSSATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLATDTFLIGNSGI 180
           SSYTPIPCSS+TCTD+TRDFPIPASCDSN LCHATLSYADASSSEG LATDTF IGNSGI
Sbjct: 121 SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180

Query: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
           SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240

Query: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSNQLLPIPESVFEPDHTGAGQ 300
           DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVS++LLPIPESVFEPDHTGAGQ
Sbjct: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 300

Query: 301 TMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVFEDPNFVFQGAMDLCYRVPLNQTRLP 360
           TMVDSGTQFTFLLGPAYTALRDEFLN+TAGS RVFED NFVFQGAMDLCYRVP+NQTRLP
Sbjct: 301 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 360

Query: 361 PLPAVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQN 420
           PLP+VTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEA+VIGHLHQQN
Sbjct: 361 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420

Query: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449
           VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450

[Close]

BLAST of Lcy06g022400 vs. ExPASy TrEMBL
Match: A0A6J1F3C7 (Laccase OS=Cucurbita moschata OX=3662 GN=LOC111441767 PE=3 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 8.9e-238
Identity = 416/450 (92.44%), Postives = 431/450 (95.78%), Query Frame = 0


Query: 1   MRDFCINFNSSNNPFLKSCFLFFF-SMILFFQNPNLCSSLNPALVLPLKTQVIPPESVRR 60
           MRD+CI FNSSN+ FLKS F FF  ++   FQN  LCSS NPAL+LPLKTQVIPPES+RR
Sbjct: 186 MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 245

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVLDTGSELSWLHCNRSQN-SSSSSSTFNPARS 120
           SPDKLPFRHNVSLTVSLTVGTPPQNVTMV+DTGSELSWLHCNRSQN SSSSSSTFNPA S
Sbjct: 246 SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 305

Query: 121 SSYTPIPCSSATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLATDTFLIGNSGI 180
           SSYTPIPCSS+TCTD+TRDFPIPASCDSN LCHATLSYADASSSEG LATDTF IGNSGI
Sbjct: 306 SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 365

Query: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
           SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 366 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 425

Query: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSNQLLPIPESVFEPDHTGAGQ 300
           DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVS++LLPIPESVFEPDHTGAGQ
Sbjct: 426 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 485

Query: 301 TMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVFEDPNFVFQGAMDLCYRVPLNQTRLP 360
           TMVDSGTQFTFLLGPAYTALRDEFLN+TAGS RVFED NFVFQGAMDLCYRVP+NQTRLP
Sbjct: 486 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 545

Query: 361 PLPAVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQN 420
           PLP+VTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEA+VIGHLHQQN
Sbjct: 546 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 605

Query: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449
           VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 606 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 635

[Close]

BLAST of Lcy06g022400 vs. ExPASy TrEMBL
Match: A0A6J1G928 (aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111452070 PE=3 SV=1)

HSP 1 Score: 832.0 bits (2148), Expect = 1.2e-237
Identity = 410/449 (91.31%), Postives = 426/449 (94.88%), Query Frame = 0


Query: 1   MRDFCINFNSSNNPFLKSCFLFF-FSMILFFQNPNLCSSLNPALVLPLKTQVIPPESVRR 60
           MRD+CI+ N  NNPFLK CF FF  S++  FQNP LC SLNPAL+LPLKTQVIPPESV R
Sbjct: 1   MRDYCISLNPLNNPFLKYCFSFFLISLVFVFQNPLLCFSLNPALILPLKTQVIPPESVPR 60

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVLDTGSELSWLHCNRSQNSSSSSSTFNPARSS 120
           SPDKLPFRHNVSLTVSLTVGTPPQNVTMV+DTGSELSWLHCNRSQNSSSSSSTFNP +SS
Sbjct: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPVQSS 120

Query: 121 SYTPIPCSSATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLATDTFLIGNSGIS 180
           SYTPIPCSS+TCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLA DTF IGNSGI 
Sbjct: 121 SYTPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSGIP 180

Query: 181 NVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD 240
           NVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDF GLLLLGD
Sbjct: 181 NVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFPGLLLLGD 240

Query: 241 ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSNQLLPIPESVFEPDHTGAGQT 300
           ANFSWLAPLNYTP+I++STPLPYFDRVAYTVQL+GIKVSN+LLPIPES FEPDHTGAGQT
Sbjct: 241 ANFSWLAPLNYTPMIQISTPLPYFDRVAYTVQLQGIKVSNKLLPIPESAFEPDHTGAGQT 300

Query: 301 MVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVFEDPNFVFQGAMDLCYRVPLNQTRLPP 360
           MVDSGTQFTFLLG AYT LRDEFLNQTAGS+RV EDPNFVFQGAMDLCYRVP NQTRLPP
Sbjct: 301 MVDSGTQFTFLLGQAYTVLRDEFLNQTAGSLRVSEDPNFVFQGAMDLCYRVPTNQTRLPP 360

Query: 361 LPAVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQNV 420
           LPAVTLVFRGAEMTVTGDRILYRV GEIRGND+I+CFTFGNSDLLGVEAYVIGHLHQQNV
Sbjct: 361 LPAVTLVFRGAEMTVTGDRILYRVAGEIRGNDTIYCFTFGNSDLLGVEAYVIGHLHQQNV 420

Query: 421 WMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449
           WMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 WMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449

[Close]

BLAST of Lcy06g022400 vs. ExPASy TrEMBL
Match: A0A6J1CW88 (aspartic proteinase PCS1 OS=Momordica charantia OX=3673 GN=LOC111014974 PE=3 SV=1)

HSP 1 Score: 830.5 bits (2144), Expect = 3.4e-237
Identity = 418/452 (92.48%), Postives = 433/452 (95.80%), Query Frame = 0


Query: 1   MRDFCINFNSSNNPFLKSCFLFFF-SMILFFQNPNLCSSLNPALVLPLKTQVIPPESVRR 60
           MRD  I FN SN+PFLKSCF FF  ++I  FQN  LCSSLNPALVLPLKTQVIPPESVRR
Sbjct: 1   MRDH-IAFNFSNSPFLKSCFFFFLCTLICGFQNTKLCSSLNPALVLPLKTQVIPPESVRR 60

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVLDTGSELSWLHCNRSQNSSS-SSSTFNPARS 120
           SPDKLPFRHNVSLTVSLTVGTPPQNVTMV+DTGSELSWLHCN+SQNSSS SSSTFNP RS
Sbjct: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNKSQNSSSNSSSTFNPIRS 120

Query: 121 SSYTPIPCSSATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLATDTFLIGNSGI 180
           SSY+PIPCSS+TCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLA DTF IGNSGI
Sbjct: 121 SSYSPIPCSSSTCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLAADTFYIGNSGI 180

Query: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
           SNVVFGCMDSIFSSNNEED+KNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 181 SNVVFGCMDSIFSSNNEEDAKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240

Query: 241 DANFSW--LAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSNQLLPIPESVFEPDHTGA 300
           DAN+SW  LAPLNYTPLI+MSTPLPYFDRVAYTV LEGIKVSN+LLPIPESVFEPDHTGA
Sbjct: 241 DANYSWLGLAPLNYTPLIQMSTPLPYFDRVAYTVHLEGIKVSNKLLPIPESVFEPDHTGA 300

Query: 301 GQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVFEDPNFVFQGAMDLCYRVPLNQTR 360
           GQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRV +DPNFVFQGAMDLCYRVPLNQ+R
Sbjct: 301 GQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVLDDPNFVFQGAMDLCYRVPLNQSR 360

Query: 361 LPPLPAVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQ 420
           LPPLPAVTLVFRGAEMTV+GDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQ
Sbjct: 361 LPPLPAVTLVFRGAEMTVSGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQ 420

Query: 421 QNVWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449
           QNVWMEFDL+KSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 QNVWMEFDLQKSRIGLAEIRCDLAGQKLGMGL 451

[Close]

BLAST of Lcy06g022400 vs. NCBI nr
Match: XP_038905417.1 (aspartic proteinase PCS1 [Benincasa hispida])

HSP 1 Score: 843.2 bits (2177), Expect = 1.0e-240
Identity = 416/449 (92.65%), Postives = 430/449 (95.77%), Query Frame = 0


Query: 1   MRDFCINFNSSNNPFLKSCFLFFF-SMILFFQNPNLCSSLNPALVLPLKTQVIPPESVRR 60
           MRD+C  FNSS+N FLK CF+FFF ++   FQN  LCSSLNPALVLPLKTQVIPPESVRR
Sbjct: 1   MRDYCFAFNSSSNKFLKYCFIFFFCTLFCVFQNSKLCSSLNPALVLPLKTQVIPPESVRR 60

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVLDTGSELSWLHCNRSQNSSSSSSTFNPARSS 120
           SPDKLPFRHN+SLTVSLTVGTPPQNVTMV+DTGSELSWLHCNRSQNSSSSSSTFNP RSS
Sbjct: 61  SPDKLPFRHNISLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPVRSS 120

Query: 121 SYTPIPCSSATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLATDTFLIGNSGIS 180
           SYTPIPCSS+TCTDQTRDFPI  SCDSNQ CHATLSYADASSSEGNLATDTF IGNSGI 
Sbjct: 121 SYTPIPCSSSTCTDQTRDFPIRPSCDSNQFCHATLSYADASSSEGNLATDTFYIGNSGIP 180

Query: 181 NVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD 240
           NVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD
Sbjct: 181 NVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD 240

Query: 241 ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSNQLLPIPESVFEPDHTGAGQT 300
           ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVS++LLPIPESVFEPDHTGAGQT
Sbjct: 241 ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQT 300

Query: 301 MVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVFEDPNFVFQGAMDLCYRVPLNQTRLPP 360
           MVDSGTQFTFLLGPAY ALRDEF+NQTAGSIRV EDPNFVFQGAMDLCYRVP+NQTRLPP
Sbjct: 301 MVDSGTQFTFLLGPAYAALRDEFVNQTAGSIRVLEDPNFVFQGAMDLCYRVPINQTRLPP 360

Query: 361 LPAVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQNV 420
           LP+VTLVFRGAEMTVTGDRILYRVPGE RGNDSIHCFTFGNSDLLGVEA+VIGHLHQQNV
Sbjct: 361 LPSVTLVFRGAEMTVTGDRILYRVPGERRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQNV 420

Query: 421 WMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449
           WMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 WMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449

[Close]

BLAST of Lcy06g022400 vs. NCBI nr
Match: XP_022983486.1 (aspartic proteinase PCS1 [Cucurbita maxima])

HSP 1 Score: 842.4 bits (2175), Expect = 1.8e-240
Identity = 419/449 (93.32%), Postives = 433/449 (96.44%), Query Frame = 0


Query: 1   MRDFCINFNSSNNPFLKSCFLFFFSMIL-FFQNPNLCSSLNPALVLPLKTQVIPPESVRR 60
           MRD+CI FNSSN+ FLKS F FF  ++   FQN  LCSSLNPAL+LPLKTQVIPPES+RR
Sbjct: 1   MRDYCIAFNSSNHKFLKSFFPFFLCILFSVFQNLILCSSLNPALLLPLKTQVIPPESIRR 60

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVLDTGSELSWLHCNRSQNSSSSSSTFNPARSS 120
           SPDKLPFRHNVSLTVSLTVGTPPQNVTMV+DTGSELSWLHCNRSQNSSSSSSTFNPA SS
Sbjct: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSTFNPAGSS 120

Query: 121 SYTPIPCSSATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLATDTFLIGNSGIS 180
           SYTPIPCSS+TCTDQTRDFPIPASCDSN LCHATLSYADASSSEG LATDTF IGNSGIS
Sbjct: 121 SYTPIPCSSSTCTDQTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGIS 180

Query: 181 NVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD 240
           NVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD
Sbjct: 181 NVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGD 240

Query: 241 ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSNQLLPIPESVFEPDHTGAGQT 300
           ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVS++LLPIPESVFEPDHTGAGQT
Sbjct: 241 ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQT 300

Query: 301 MVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVFEDPNFVFQGAMDLCYRVPLNQTRLPP 360
           MVDSGTQFTFLLGPAYTALRDEFLN+TAGSIRVFED NFVFQGAMDLCYRVP+NQTRLPP
Sbjct: 301 MVDSGTQFTFLLGPAYTALRDEFLNRTAGSIRVFEDSNFVFQGAMDLCYRVPMNQTRLPP 360

Query: 361 LPAVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQNV 420
           LP+VTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEA+VIGHLHQQNV
Sbjct: 361 LPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQNV 420

Query: 421 WMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449
           WMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 WMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449

[Close]

BLAST of Lcy06g022400 vs. NCBI nr
Match: XP_023528618.1 (aspartic proteinase PCS1 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 832.8 bits (2150), Expect = 1.4e-237
Identity = 416/450 (92.44%), Postives = 431/450 (95.78%), Query Frame = 0


Query: 1   MRDFCINFNSSNNPFLKSCFLFFF-SMILFFQNPNLCSSLNPALVLPLKTQVIPPESVRR 60
           MRD+CI FNSSN+ FLKS F FF  ++   FQN  LCSS NPAL+LPLKTQVIPPES+RR
Sbjct: 1   MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 60

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVLDTGSELSWLHCNRSQN-SSSSSSTFNPARS 120
           SPDKLPFRHNVSLTVSLTVGTPPQNVTMV+DTGSELSWLHCNRSQN SSSSSSTFNPA S
Sbjct: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 120

Query: 121 SSYTPIPCSSATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLATDTFLIGNSGI 180
           SSYTPIPCSS+TCTDQTRDFPIPASCDSN LCHATLSYADASSSEG LATDTF IGNSGI
Sbjct: 121 SSYTPIPCSSSTCTDQTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180

Query: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
           SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240

Query: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSNQLLPIPESVFEPDHTGAGQ 300
           DANFSWLAPLNYTPLIEM+TPLPYFDRVAYTVQLEGIKVS++LLPIPESVFEPDHTGAGQ
Sbjct: 241 DANFSWLAPLNYTPLIEMTTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 300

Query: 301 TMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVFEDPNFVFQGAMDLCYRVPLNQTRLP 360
           TMVDSGTQFTFLLGPAYTALRDEFLN+TAGS RVFED NFVFQGAMDLCYRVP+NQTRLP
Sbjct: 301 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 360

Query: 361 PLPAVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQN 420
           PLP+VTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEA+VIGHLHQQN
Sbjct: 361 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420

Query: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449
           VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450

[Close]

BLAST of Lcy06g022400 vs. NCBI nr
Match: XP_023528617.1 (aspartic proteinase PCS1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 832.8 bits (2150), Expect = 1.4e-237
Identity = 416/450 (92.44%), Postives = 431/450 (95.78%), Query Frame = 0


Query: 1   MRDFCINFNSSNNPFLKSCFLFFF-SMILFFQNPNLCSSLNPALVLPLKTQVIPPESVRR 60
           MRD+CI FNSSN+ FLKS F FF  ++   FQN  LCSS NPAL+LPLKTQVIPPES+RR
Sbjct: 55  MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 114

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVLDTGSELSWLHCNRSQN-SSSSSSTFNPARS 120
           SPDKLPFRHNVSLTVSLTVGTPPQNVTMV+DTGSELSWLHCNRSQN SSSSSSTFNPA S
Sbjct: 115 SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 174

Query: 121 SSYTPIPCSSATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLATDTFLIGNSGI 180
           SSYTPIPCSS+TCTDQTRDFPIPASCDSN LCHATLSYADASSSEG LATDTF IGNSGI
Sbjct: 175 SSYTPIPCSSSTCTDQTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 234

Query: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
           SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 235 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 294

Query: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSNQLLPIPESVFEPDHTGAGQ 300
           DANFSWLAPLNYTPLIEM+TPLPYFDRVAYTVQLEGIKVS++LLPIPESVFEPDHTGAGQ
Sbjct: 295 DANFSWLAPLNYTPLIEMTTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 354

Query: 301 TMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVFEDPNFVFQGAMDLCYRVPLNQTRLP 360
           TMVDSGTQFTFLLGPAYTALRDEFLN+TAGS RVFED NFVFQGAMDLCYRVP+NQTRLP
Sbjct: 355 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 414

Query: 361 PLPAVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQN 420
           PLP+VTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEA+VIGHLHQQN
Sbjct: 415 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 474

Query: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449
           VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 475 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 504

[Close]

BLAST of Lcy06g022400 vs. NCBI nr
Match: XP_022935018.1 (aspartic proteinase PCS1 [Cucurbita moschata] >KAG6580532.1 Aspartic proteinase PCS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 832.4 bits (2149), Expect = 1.8e-237
Identity = 416/450 (92.44%), Postives = 431/450 (95.78%), Query Frame = 0


Query: 1   MRDFCINFNSSNNPFLKSCFLFFF-SMILFFQNPNLCSSLNPALVLPLKTQVIPPESVRR 60
           MRD+CI FNSSN+ FLKS F FF  ++   FQN  LCSS NPAL+LPLKTQVIPPES+RR
Sbjct: 1   MRDYCIAFNSSNHKFLKSLFPFFLCTLFSVFQNLILCSSQNPALLLPLKTQVIPPESIRR 60

Query: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVLDTGSELSWLHCNRSQN-SSSSSSTFNPARS 120
           SPDKLPFRHNVSLTVSLTVGTPPQNVTMV+DTGSELSWLHCNRSQN SSSSSSTFNPA S
Sbjct: 61  SPDKLPFRHNVSLTVSLTVGTPPQNVTMVIDTGSELSWLHCNRSQNSSSSSSSTFNPAGS 120

Query: 121 SSYTPIPCSSATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLATDTFLIGNSGI 180
           SSYTPIPCSS+TCTD+TRDFPIPASCDSN LCHATLSYADASSSEG LATDTF IGNSGI
Sbjct: 121 SSYTPIPCSSSTCTDRTRDFPIPASCDSNHLCHATLSYADASSSEGTLATDTFYIGNSGI 180

Query: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240
           SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG
Sbjct: 181 SNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLG 240

Query: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSNQLLPIPESVFEPDHTGAGQ 300
           DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVS++LLPIPESVFEPDHTGAGQ
Sbjct: 241 DANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSHKLLPIPESVFEPDHTGAGQ 300

Query: 301 TMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVFEDPNFVFQGAMDLCYRVPLNQTRLP 360
           TMVDSGTQFTFLLGPAYTALRDEFLN+TAGS RVFED NFVFQGAMDLCYRVP+NQTRLP
Sbjct: 301 TMVDSGTQFTFLLGPAYTALRDEFLNRTAGSFRVFEDSNFVFQGAMDLCYRVPMNQTRLP 360

Query: 361 PLPAVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQN 420
           PLP+VTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEA+VIGHLHQQN
Sbjct: 361 PLPSVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAFVIGHLHQQN 420

Query: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 449
           VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL
Sbjct: 421 VWMEFDLKKSRIGLAEIRCDLAGQKLGMGL 450

[Close]

BLAST of Lcy06g022400 vs. TAIR 10
Match: AT5G02190.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 576.2 bits (1484), Expect = 2.2e-164
Identity = 288/416 (69.23%), Postives = 342/416 (82.21%), Query Frame = 0


Query: 37  SSLNPALVLPLKTQVIPPESVRRSPDKLPFRHNVSLTVSLTVGTPPQNVTMVLDTGSELS 96
           SS +  LVLPLKT++ P +   R  DKL F HNV+LTV+LTVGTPPQN++MV+DTGSELS
Sbjct: 40  SSSSQTLVLPLKTRITPTD--HRPTDKLHFHHNVTLTVTLTVGTPPQNISMVIDTGSELS 99

Query: 97  WLHCNRSQNSSSSSSTFNPARSSSYTPIPCSSATCTDQTRDFPIPASCDSNQLCHATLSY 156
           WL CNRS N +  ++ F+P RSSSY+PIPCSS TC  +TRDF IPASCDS++LCHATLSY
Sbjct: 100 WLRCNRSSNPNPVNN-FDPTRSSSYSPIPCSSPTCRTRTRDFLIPASCDSDKLCHATLSY 159

Query: 157 ADASSSEGNLATDTFLIGNS-GISNVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVS 216
           ADASSSEGNLA + F  GNS   SN++FGCM S+  S+ EED+K TGL+GMNRGSLSF+S
Sbjct: 160 ADASSSEGNLAAEIFHFGNSTNDSNLIFGCMGSVSGSDPEEDTKTTGLLGMNRGSLSFIS 219

Query: 217 QMGFPKFSYCIS-EYDFSGLLLLGDANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEG 276
           QMGFPKFSYCIS   DF G LLLGD+NF+WL PLNYTPLI +STPLPYFDRVAYTVQL G
Sbjct: 220 QMGFPKFSYCISGTDDFPGFLLLGDSNFTWLTPLNYTPLIRISTPLPYFDRVAYTVQLTG 279

Query: 277 IKVSNQLLPIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVFE 336
           IKV+ +LLPIP+SV  PDHTGAGQTMVDSGTQFTFLLGP YTALR  FLN+T G + V+E
Sbjct: 280 IKVNGKLLPIPKSVLVPDHTGAGQTMVDSGTQFTFLLGPVYTALRSHFLNRTNGILTVYE 339

Query: 337 DPNFVFQGAMDLCYRVPLNQTR---LPPLPAVTLVFRGAEMTVTGDRILYRVPGEIRGND 396
           DP+FVFQG MDLCYR+   + R   L  LP V+LVF GAE+ V+G  +LYRVP    GND
Sbjct: 340 DPDFVFQGTMDLCYRISPVRIRSGILHRLPTVSLVFEGAEIAVSGQPLLYRVPHLTVGND 399

Query: 397 SIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLKKSRIGLAEIRCDLAGQKLGMG 448
           S++CFTFGNSDL+G+EAYVIGH HQQN+W+EFDL++SRIGLA + CD++GQ+LG+G
Sbjct: 400 SVYCFTFGNSDLMGMEAYVIGHHHQQNMWIEFDLQRSRIGLAPVECDVSGQRLGIG 452

[Close]

BLAST of Lcy06g022400 vs. TAIR 10
Match: AT2G39710.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 536.6 bits (1381), Expect = 1.9e-152
Identity = 275/444 (61.94%), Postives = 338/444 (76.13%), Query Frame = 0


Query: 9   NSSNNPFLKSCFLFFFSMILFFQNPNLC--SSLNPALVLPLKTQVIPPESVRRSPDKLPF 68
           +SS++  L   FL    ++L F     C  SS N  L+  LKTQ +P    + S DKL F
Sbjct: 5   SSSSSLSLSKNFLRISVLLLIFP-LTFCKTSSTNQTLLFSLKTQKLP----QSSSDKLSF 64

Query: 69  RHNVSLTVSLTVGTPPQNVTMVLDTGSELSWLHCNRSQNSSSSSSTFNPARSSSYTPIPC 128
           RHNV+LTV+L VG PPQN++MVLDTGSELSWLHC +S N     S FNP  SS+Y+P+PC
Sbjct: 65  RHNVTLTVTLAVGDPPQNISMVLDTGSELSWLHCKKSPN---LGSVFNPVSSSTYSPVPC 124

Query: 129 SSATCTDQTRDFPIPASCD-SNQLCHATLSYADASSSEGNLATDTFLIGNSGISNVVFGC 188
           SS  C  +TRD PIPASCD    LCH  +SYADA+S EGNLA +TF+IG+      +FGC
Sbjct: 125 SSPICRTRTRDLPIPASCDPKTHLCHVAISYADATSIEGNLAHETFVIGSVTRPGTLFGC 184

Query: 189 MDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYDFSGLLLLGDANFSWL 248
           MDS  SSN+EED+K+TGLMGMNRGSLSFV+Q+GF KFSYCIS  D SG LLLGDA++SWL
Sbjct: 185 MDSGLSSNSEEDAKSTGLMGMNRGSLSFVNQLGFSKFSYCISGSDSSGFLLLGDASYSWL 244

Query: 249 APLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSNQLLPIPESVFEPDHTGAGQTMVDSGT 308
            P+ YTPL+  STPLPYFDRVAYTVQLEGI+V +++L +P+SVF PDHTGAGQTMVDSGT
Sbjct: 245 GPIQYTPLVLQSTPLPYFDRVAYTVQLEGIRVGSKILSLPKSVFVPDHTGAGQTMVDSGT 304

Query: 309 QFTFLLGPAYTALRDEFLNQTAGSIRVFEDPNFVFQGAMDLCYRV-PLNQTRLPPLPAVT 368
           QFTFL+GP YTAL++EF+ QT   +R+ +DP+FVFQG MDLCY+V    +     LP V+
Sbjct: 305 QFTFLMGPVYTALKNEFITQTKSVLRLVDDPDFVFQGTMDLCYKVGSTTRPNFSGLPMVS 364

Query: 369 LVFRGAEMTVTGDRILYRVPGE-IRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEF 428
           L+FRGAEM+V+G ++LYRV G    G + ++CFTFGNSDLLG+EA+VIGH HQQNVWMEF
Sbjct: 365 LMFRGAEMSVSGQKLLYRVNGAGSEGKEEVYCFTFGNSDLLGIEAFVIGHHHQQNVWMEF 424

Query: 429 DLKKSRIGLA-EIRCDLAGQKLGM 447
           DL KSR+G A  +RCDLA Q+LG+
Sbjct: 425 DLAKSRVGFAGNVRCDLASQRLGL 440

[Close]

BLAST of Lcy06g022400 vs. TAIR 10
Match: AT1G66180.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 250.0 bits (637), Expect = 3.6e-66
Identity = 162/452 (35.84%), Postives = 242/452 (53.54%), Query Frame = 0


Query: 13  NPFLKSCFLFFFSMILFFQNPNLCSSLN-PALVLPLKTQVIPPESV-----RRSPD---- 72
           +P  K  F FFF   L + + +   SL+ P   LP+ T             R++P     
Sbjct: 2   SPIPKPLFFFFF---LNYVSLSTSLSLHLPLTSLPISTTTNSHRFTTSLLSRKNPSPSSP 61

Query: 73  ----KLPFRHNVSLTVSLTVGTPPQNVTMVLDTGSELSWLHCNRSQNSSSSSSTFNPARS 132
               +  F+++++L +SL +GTPPQ   MVLDTGS+LSW+ C+R +      ++F+P+ S
Sbjct: 62  PYNFRSRFKYSMALIISLPIGTPPQAQQMVLDTGSQLSWIQCHRKKLPPKPKTSFDPSLS 121

Query: 133 SSYTPIPCSSATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLATDTFLIGNSGI 192
           SS++ +PCS   C  +  DF +P SCDSN+LCH +  YAD + +EGNL  +     N+ I
Sbjct: 122 SSFSTLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKITFSNTEI 181

Query: 193 S-NVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISE------YDF 252
           +  ++ GC          E S + G++GMNRG LSFVSQ    KFSYCI        +  
Sbjct: 182 TPPLILGCA--------TESSDDRGILGMNRGRLSFVSQAKISKFSYCIPPKSNRPGFTP 241

Query: 253 SGLLLLGD----ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSNQLLPIPES 312
           +G   LGD      F +++ L +      S  +P  D +AYTV + GI+   + L I  S
Sbjct: 242 TGSFYLGDNPNSHGFKYVSLLTFPE----SQRMPNLDPLAYTVPMIGIRFGLKKLNISGS 301

Query: 313 VFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVFEDPNFVFQGAMDLC 372
           VF PD  G+GQTMVDSG++FT L+  AY  +R E + +    ++      +V+ G  D+C
Sbjct: 302 VFRPDAGGSGQTMVDSGSEFTHLVDAAYDKVRAEIMTRVGRRLK----KGYVYGGTADMC 361

Query: 373 YRVPLNQTRLPPL--PAVTLVFRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLG 432
           +    N   +P L    V +  RG E+ V  +R+L  V G       IHC   G S +LG
Sbjct: 362 F--DGNVAMIPRLIGDLVFVFTRGVEILVPKERVLVNVGG------GIHCVGIGRSSMLG 421

Query: 433 VEAYVIGHLHQQNVWMEFDLKKSRIGLAEIRC 438
             + +IG++HQQN+W+EFD+   R+G A+  C
Sbjct: 422 AASNIIGNVHQQNLWVEFDVTNRRVGFAKADC 426

[Close]

BLAST of Lcy06g022400 vs. TAIR 10
Match: AT5G37540.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 237.3 bits (604), Expect = 2.4e-62
Identity = 158/460 (34.35%), Postives = 243/460 (52.83%), Query Frame = 0


Query: 15  FLKSCFLFFFSMILFFQNPNLCSSLNPALVLPLKTQVIPPES----------VRR--SPD 74
           FLK  ++FFF    F  + +L  S + +L  PL +  + P +           RR  SP 
Sbjct: 9   FLKLLYIFFF----FCYSVSLSWSSSLSLHFPLTSLRLTPTTNSSSFKTSLLSRRNPSPP 68

Query: 75  KLPF------RHNVSLTVSLTVGTPPQNVTMVLDTGSELSWLHCNRSQNSS---SSSSTF 134
             P+      +++++L +SL +GTP Q+  +VLDTGS+LSW+ C+  +        +++F
Sbjct: 69  SSPYTFRSNIKYSMALILSLPIGTPSQSQELVLDTGSQLSWIQCHPKKIKKPLPPPTTSF 128

Query: 135 NPARSSSYTPIPCSSATCTDQTRDFPIPASCDSNQLCHATLSYADASSSEGNLATDTFLI 194
           +P+ SSS++ +PCS   C  +  DF +P SCDSN+LCH +  YAD + +EGNL  + F  
Sbjct: 129 DPSLSSSFSDLPCSHPLCKPRIPDFTLPTSCDSNRLCHYSYFYADGTFAEGNLVKEKFTF 188

Query: 195 GNSGIS-NVVFGCMDSIFSSNNEEDSKNTGLMGMNRGSLSFVSQMGFPKFSYCISEYD-- 254
            NS  +  ++ GC         +E +   G++GMN G LSF+SQ    KFSYCI      
Sbjct: 189 SNSQTTPPLILGCA--------KESTDEKGILGMNLGRLSFISQAKISKFSYCIPTRSNR 248

Query: 255 ----FSGLLLLGD----ANFSWLAPLNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSNQLL 314
                +G   LGD      F +++ L +      S  +P  D +AYTV L+GI++  + L
Sbjct: 249 PGLASTGSFYLGDNPNSRGFKYVSLLTF----PQSQRMPNLDPLAYTVPLQGIRIGQKRL 308

Query: 315 PIPESVFEPDHTGAGQTMVDSGTQFTFLLGPAYTALRDEFLNQTAGSIRVFEDPNFVFQG 374
            IP SVF PD  G+GQTMVDSG++FT L+  AY  +++E +      ++      +V+  
Sbjct: 309 NIPGSVFRPDAGGSGQTMVDSGSEFTHLVDVAYDKVKEEIVRLVGSRLK----KGYVYGS 368

Query: 375 AMDLCYRVPLNQTRLPPLPAVTLVF---RGAEMTVTGDRILYRVPGEIRGNDSIHCFTFG 434
             D+C+    N +         LVF   RG E+ V    +L  V G       IHC   G
Sbjct: 369 TADMCF--DGNHSMEIGRLIGDLVFEFGRGVEILVEKQSLLVNVGG------GIHCVGIG 428

Query: 435 NSDLLGVEAYVIGHLHQQNVWMEFDLKKSRIGLAEIRCDL 440
            S +LG  + +IG++HQQN+W+EFD+   R+G ++  C L
Sbjct: 429 RSSMLGAASNIIGNVHQQNLWVEFDVTNRRVGFSKAECRL 440

[Close]

BLAST of Lcy06g022400 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 144.8 bits (364), Expect = 1.6e-34
Identity = 122/371 (32.88%), Postives = 179/371 (48.25%), Query Frame = 0


Query: 76  LTVGTPPQNVTMVLDTGSELSWLHCNRSQNS-SSSSSTFNPARSSSYTPIPCSSATCTDQ 135
           L VGTP + V MVLDTGS++ WL C   +   S S   F+P +S +Y  IPCSS  C   
Sbjct: 146 LGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCSSPHCRRL 205

Query: 136 TRDFPIPASCDS-NQLCHATLSYADASSSEGNLATDTFLIGNSGISNVVFGCMDSIFSSN 195
                  A C++  + C   +SY D S + G+ +T+T     + +  V  GC       N
Sbjct: 206 D-----SAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGC----GHDN 265

Query: 196 NEEDSKNTGLMGMNRGSLSFVSQMGF---PKFSYCISEYDFS---GLLLLGDANFSWLAP 255
                   GL+G+ +G LSF  Q G     KFSYC+ +   S     ++ G+A  S +A 
Sbjct: 266 EGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIA- 325

Query: 256 LNYTPLIEMSTPLPYFDRVAYTVQLEGIKVSNQLLP-IPESVFEPDHTGAGQTMVDSGTQ 315
             +TPL+      P  D   Y V L GI V    +P +  S+F+ D  G G  ++DSGT 
Sbjct: 326 -RFTPLLSN----PKLD-TFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTS 385

Query: 316 FTFLLGPAYTALRDEFLNQTAGSIRVFEDPNFVFQGAMDLCYRVPLNQTRLPPLPAVTLV 375
            T L+ PAY A+RD F     G+  +   P+F      D C+   L+      +P V L 
Sbjct: 386 VTRLIRPAYIAMRDAF---RVGAKTLKRAPDF---SLFDTCF--DLSNMNEVKVPTVVLH 445

Query: 376 FRGAEMTVTGDRILYRVPGEIRGNDSIHCFTFGNSDLLGVEAYVIGHLHQQNVWMEFDLK 435
           FRGA++++      Y +P +  G     CF F  + + G+   +IG++ QQ   + +DL 
Sbjct: 446 FRGADVSLPATN--YLIPVDTNGK---FCFAFAGT-MGGLS--IIGNIQQQGFRVVYDLA 484

Query: 436 KSRIGLAEIRC 438
            SR+G A   C
Sbjct: 506 SSRVGFAPGGC 484

The following BLAST results are available for this feature:

BLAST of Lcy06g022400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZL3	3.1e-163	69.23	Aspartic proteinase PCS1 OS=Arabidopsis thaliana OX=3702 GN=PCS1 PE=2 SV=1	[more]
Q766C3	1.8e-38	32.16	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]
Q766C2	1.0e-36	29.16	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q9LNJ3	2.3e-33	32.88	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
O04496	6.7e-33	31.48	Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1	[more]

BLAST of Lcy06g022400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J2F5	8.6e-241	93.32	aspartic proteinase PCS1 OS=Cucurbita maxima OX=3661 GN=LOC111482077 PE=3 SV=1	[more]
A0A6J1F9C1	8.9e-238	92.44	aspartic proteinase PCS1 OS=Cucurbita moschata OX=3662 GN=LOC111442007 PE=3 SV=1	[more]
A0A6J1F3C7	8.9e-238	92.44	Laccase OS=Cucurbita moschata OX=3662 GN=LOC111441767 PE=3 SV=1	[more]
A0A6J1G928	1.2e-237	91.31	aspartic proteinase PCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111452070 PE=3...	[more]
A0A6J1CW88	3.4e-237	92.48	aspartic proteinase PCS1 OS=Momordica charantia OX=3673 GN=LOC111014974 PE=3 SV=...	[more]

BLAST of Lcy06g022400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905417.1	1.0e-240	92.65	aspartic proteinase PCS1 [Benincasa hispida]	[more]
XP_022983486.1	1.8e-240	93.32	aspartic proteinase PCS1 [Cucurbita maxima]	[more]
XP_023528618.1	1.4e-237	92.44	aspartic proteinase PCS1 isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_023528617.1	1.4e-237	92.44	aspartic proteinase PCS1 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022935018.1	1.8e-237	92.44	aspartic proteinase PCS1 [Cucurbita moschata] >KAG6580532.1 Aspartic proteinase ...	[more]

BLAST of Lcy06g022400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G02190.1	2.2e-164	69.23	Eukaryotic aspartyl protease family protein	[more]
AT2G39710.1	1.9e-152	61.94	Eukaryotic aspartyl protease family protein	[more]
AT1G66180.1	3.6e-66	35.84	Eukaryotic aspartyl protease family protein	[more]
AT5G37540.1	2.4e-62	34.35	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	1.6e-34	32.88	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 299..310 score: 32.91 coord: 409..424 score: 18.07 coord: 78..98 score: 46.74
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR47965	ASPARTYL PROTEASE-RELATED	coord: 21..446
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 74..239 e-value: 7.8E-40 score: 137.0
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 243..440 e-value: 1.9E-43 score: 150.2
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 63..239 e-value: 6.7E-37 score: 129.3
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 73..437
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 267..432 e-value: 4.8E-36 score: 124.0
None	No IPR available	PANTHER	PTHR47965:SF64	ASPARTIC PROTEINASE PCS1	coord: 21..446
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 87..98
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 72..433 score: 36.210102
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 73..437 e-value: 8.28618E-71 score: 222.909

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy06g022400.1	Lcy06g022400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046274	lignin catabolic process
biological_process	GO:0012501	programmed cell death
biological_process	GO:0006508	proteolysis
cellular_component	GO:0048046	apoplast
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004190	aspartic-type endopeptidase activity
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0052716	hydroquinone:oxygen oxidoreductase activity