Lcy06g020960 (gene) Sponge gourd (P93075) v1

Overview
NameLcy06g020960
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRNase H domain-containing protein
LocationChr06: 45947748 .. 45948433 (-)
RNA-Seq ExpressionLcy06g020960
SyntenyLcy06g020960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGTATGCTCGTTGTGCTTGGAGGTACCTGAAACATCAGACCACACACTTTTTCAGTGTCATAGAGCAAAGGAAGTGTGGACAAGGTTGGATGTGGGAGGTCAAATTGGATGGGAAAGAGACATGGACATTAAAGATAAATGGATCCATTTAGCTGACTCTGTTCCGGATACAGTTTTAGAGCGAATTTGTGTGGGGTGTTGGGCTATTTGGAATGATCGTAATAACACCATTCATAATCATCCTGTTCCTCCTGTTAATGTTCGTTGTGAATGGATTGATGATTATTTGTATGATTTTAAGACGACAAATCAGCGTATTGGTCGGAAGGCCCAGTCGATCCAAGATATTAACAGGTTGATTTCTCAAGGAAAAAAATACATAATGCATGTGGATGCAGCATGGGACGTGGTGAATAAGAAAAGTGCTATTGGTGTGGTGCTTCGTGACCATAAGGGCCAGTTAAAGCTAGCAATGCATAAGGGAGTTCCTATTTTTTCGTCACCTTTGTGTGCTGAGGTTGTCGTAGTGTTTGAGGGCCTTAAGCTTGCGCGTCGGAATGATGTCAAAAGTTTGTCGCTTTCTCCACGTTTGTGGTCGTCGAACTTTCCTGGTTGGATTGTTCAACAAGCTCAATATGAGCTTCAGTATTTTGTATCCCCTAGGGGAAGTATTTTGAGTTAA

mRNA sequence

ATGGGGTATGCTCGTTGTGCTTGGAGAGCAAAGGAAGTGTGGACAAGGTTGGATGTGGGAGGTCAAATTGGATGGGAAAGAGACATGGACATTAAAGATAAATGGATCCATTTAGCTGACTCTGTTCCGGATACAGTTTTAGAGCGAATTTGTGTGGGGTGTTGGGCTATTTGGAATGATCGTAATAACACCATTCATAATCATCCTGTTCCTCCTGTTAATGTTCGTTGTGAATGGATTGATGATTATTTGTATGATTTTAAGACGACAAATCAGCGTATTGGTCGGAAGGCCCAGTCGATCCAAGATATTAACAGGTTGATTTCTCAAGGAAAAAAATACATAATGCATGTGGATGCAGCATGGGACGTGGTGAATAAGAAAAGTGCTATTGGTGTGGTGCTTCGTGACCATAAGGGCCAGTTAAAGCTAGCAATGCATAAGGGAGTTCCTATTTTTTCGTCACCTTTGTGTGCTGAGGTTGTCGTAGTGTTTGAGGGCCTTAAGCTTGCGCGTCGGAATGATGTCAAAAGTTTGTCGCTTTCTCCACGTTTGTGGTCGTCGAACTTTCCTGGTTGGATTGTTCAACAAGCTCAATATGAGCTTCAGTATTTTGTATCCCCTAGGGGAAGTATTTTGAGTTAA

Coding sequence (CDS)

ATGGGGTATGCTCGTTGTGCTTGGAGAGCAAAGGAAGTGTGGACAAGGTTGGATGTGGGAGGTCAAATTGGATGGGAAAGAGACATGGACATTAAAGATAAATGGATCCATTTAGCTGACTCTGTTCCGGATACAGTTTTAGAGCGAATTTGTGTGGGGTGTTGGGCTATTTGGAATGATCGTAATAACACCATTCATAATCATCCTGTTCCTCCTGTTAATGTTCGTTGTGAATGGATTGATGATTATTTGTATGATTTTAAGACGACAAATCAGCGTATTGGTCGGAAGGCCCAGTCGATCCAAGATATTAACAGGTTGATTTCTCAAGGAAAAAAATACATAATGCATGTGGATGCAGCATGGGACGTGGTGAATAAGAAAAGTGCTATTGGTGTGGTGCTTCGTGACCATAAGGGCCAGTTAAAGCTAGCAATGCATAAGGGAGTTCCTATTTTTTCGTCACCTTTGTGTGCTGAGGTTGTCGTAGTGTTTGAGGGCCTTAAGCTTGCGCGTCGGAATGATGTCAAAAGTTTGTCGCTTTCTCCACGTTTGTGGTCGTCGAACTTTCCTGGTTGGATTGTTCAACAAGCTCAATATGAGCTTCAGTATTTTGTATCCCCTAGGGGAAGTATTTTGAGTTAA

Protein sequence

MGYARCAWRAKEVWTRLDVGGQIGWERDMDIKDKWIHLADSVPDTVLERICVGCWAIWNDRNNTIHNHPVPPVNVRCEWIDDYLYDFKTTNQRIGRKAQSIQDINRLISQGKKYIMHVDAAWDVVNKKSAIGVVLRDHKGQLKLAMHKGVPIFSSPLCAEVVVVFEGLKLARRNDVKSLSLSPRLWSSNFPGWIVQQAQYELQYFVSPRGSILS
Homology
BLAST of Lcy06g020960 vs. ExPASy TrEMBL
Match: A0A6J1BQ49 (uncharacterized protein LOC111004786 OS=Momordica charantia OX=3673 GN=LOC111004786 PE=4 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 2.3e-10
Identity = 48/172 (27.91%), Postives = 80/172 (46.51%), Query Frame = 0

Query: 9   RAKEVWTRL-------DVGGQIGWERDMDIKDKWIHLADSVPDTVLERICVGCWAIWNDR 68
           RAK++W  +        + G + +   MDI   W+ L D +     +      WAIW DR
Sbjct: 13  RAKKIWNLMFPSLFTSPINGNLSF---MDI---WLLLYDKLNPQQRDLAAFTXWAIWCDR 72

Query: 69  NNTIHNHPVPPVNVRCEWIDDYLYDFKTT--NQRIGRKAQSIQDINRLISQGKKYIMHVD 128
           N+  H   V    +RC WI  Y  ++     N+RI  +  S+   +      +   M V+
Sbjct: 73  NSXAHGSSVSTPALRCNWISSYFQNYSQAQENKRISPQQSSVPPPSCRWLPPRDSAMKVN 132

Query: 129 AAWDVVNKKSAIGVVLRDHKGQLKLAMHKGVPIFSSPLCAEVVVVFEGLKLA 172
           +     +  + +G+++RDH G L +A    +P+  +PL AE+  + E LKLA
Sbjct: 133 SDAACRSTSTGLGLIIRDHFGVLLVAKSMFLPMPLNPLFAEIRGILEALKLA 178

BLAST of Lcy06g020960 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 3.0e-10
Identity = 48/180 (26.67%), Postives = 79/180 (43.89%), Query Frame = 0

Query: 9    RAKEVW-TRLDVGGQIGWERDMDIKDKWIHLADSVPDTVLERICVGCWAIWNDRNNTIHN 68
            RA+++W T       +  E ++   + W  L + +    L    +  W IWNDRN+ IH 
Sbjct: 1192 RARQIWRTLFPFLTCLSAEDNISFLELWSSLTEQLEPKDLNLAAITGWGIWNDRNSLIHG 1251

Query: 69   HPVPPVNVRCEWIDDYLYDFKTTNQRIGRKAQSIQDINRLI------SQGKKYIMHVDAA 128
              V PV  +CEW+  +L     +  ++   +   Q  +R +      S      ++ DAA
Sbjct: 1252 KQVSPVEFKCEWLTPFLDSH--SQAQMSNYSPRTQSNHRPVVQYWRPSSSVSLKLNTDAA 1311

Query: 129  WDVVNKKSAIGVVLRDHKGQLKLAMHKGVPIFSSPLCAEVVVVFEGLKLARRNDVKSLSL 182
                   ++ G ++RD    L  A    VP   SPL AE+  + EGLK A  ++   L +
Sbjct: 1312 --CRGASTSFGCIIRDSSCSLVAATSIRVPFPLSPLLAEIRCILEGLKFAAASNFTHLEV 1367

BLAST of Lcy06g020960 vs. ExPASy TrEMBL
Match: A0A6J1CK80 (uncharacterized protein LOC111012047 OS=Momordica charantia OX=3673 GN=LOC111012047 PE=4 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 6.8e-10
Identity = 42/154 (27.27%), Postives = 80/154 (51.95%), Query Frame = 0

Query: 23  IGWERDMDIKDKWIHLADSVPDTVLERICVG--CWAIWNDRNNTIHNHPVPPVNVRCEWI 82
           +G + +    D+W    +++  T+ E +  G  CWA WNDRN   +   V  V+   +WI
Sbjct: 4   LGVDCNCSFIDRWALWRENL--TLEELLLAGIICWANWNDRNQRNNGIDVADVHTESDWI 63

Query: 83  DDYLYDFKTTNQRIGRKAQSIQDINRLISQGKKYI-MHVDAAWDVVNKKSAIGVVLRDHK 142
            +Y  +  T  ++     +++  +       K  + ++VDAA+DV N++  +GV++RD +
Sbjct: 64  TNYAMELLTRRRKTNEAQKNLPCLAIWKPPSKGTVKLNVDAAFDVRNQRGGVGVLVRDDR 123

Query: 143 GQLKLAMHKGVPIFSSPLCAEVVVVFEGLKLARR 174
             +  A+     + +SPL AE+  + E ++LA R
Sbjct: 124 NSILAALISSHNV-NSPLLAEICAIREAVRLAER 154

BLAST of Lcy06g020960 vs. ExPASy TrEMBL
Match: A0A6J1CTE3 (uncharacterized protein LOC111014578 OS=Momordica charantia OX=3673 GN=LOC111014578 PE=4 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.5e-09
Identity = 51/174 (29.31%), Postives = 78/174 (44.83%), Query Frame = 0

Query: 9   RAKEVWTRLDVGGQIGWERDMDIKDKWIHLADSVPDTVLERICVGCWAIWNDRNNTIHNH 68
           RAKEVW  L        + +  ++D  ++L +S+     + + VG WAIWNDRN      
Sbjct: 47  RAKEVWNILLPQAFPQCDFNNSVQDGLLYLVESLSTPDFDLVGVGVWAIWNDRNAIRMQR 106

Query: 69  PVPPVNVRCEWIDDYLYDFKTTNQRIGRKAQSIQD--INRLISQGKKYI----------- 128
            +P   +R +WI  Y+ DF+       R   S+ D  I    S   + I           
Sbjct: 107 QIPDAKIRSDWILTYVRDFQV------RDVPSLGDFRIQEDASSNDRTIENYWSPPPAGW 166

Query: 129 --MHVDAAWDVVNKKSAIGVVLRDHKGQLKLAMHKGVPIFSSPLCAEVVVVFEG 168
             ++VDAA      ++ IG+V R+ KGQ+  A          PL AE + +F+G
Sbjct: 167 IKINVDAACKKHQFRTGIGIVCRNEKGQILAAAS------HDPLMAESLALFDG 208

BLAST of Lcy06g020960 vs. ExPASy TrEMBL
Match: A0A7J9BAK4 (RNase H domain-containing protein (Fragment) OS=Gossypium gossypioides OX=34282 GN=Gogos_017354 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 4.4e-09
Identity = 40/136 (29.41%), Postives = 71/136 (52.21%), Query Frame = 0

Query: 51  CVGCWAIWNDRNNTIHNHPVPPVNVRCEWIDDYLYDFKTTNQRIGRKAQSIQD--INRLI 110
           C   W IW+ RN T+H        V  + I+ YL +     + +G+K  ++++  I RL 
Sbjct: 32  CCALWVIWSSRNQTVHERKHASGRVLAQKIESYLAEL----EGVGKKKCTLKNARIQRLR 91

Query: 111 SQGKKYIMHVDAAWDVVNKKSAIGVVLRDHKGQL---KLAMHKGVPIFSSPLCAEVVVVF 170
            +  +  +H DAA+D    +SA GV++RD +G++   K  +H  V   SSP  AE +   
Sbjct: 92  EEITEDTIHFDAAFDTNRSRSASGVIVRDRRGEMRVSKTTLHSNV---SSPFLAEALTCL 151

Query: 171 EGLKLARRNDVKSLSL 182
           + +KL     ++S+++
Sbjct: 152 QAIKLGISLGLRSVTI 160

BLAST of Lcy06g020960 vs. NCBI nr
Match: XP_022131661.1 (uncharacterized protein LOC111004786 [Momordica charantia])

HSP 1 Score: 75.9 bits (185), Expect = 4.8e-10
Identity = 48/172 (27.91%), Postives = 80/172 (46.51%), Query Frame = 0

Query: 9   RAKEVWTRL-------DVGGQIGWERDMDIKDKWIHLADSVPDTVLERICVGCWAIWNDR 68
           RAK++W  +        + G + +   MDI   W+ L D +     +      WAIW DR
Sbjct: 13  RAKKIWNLMFPSLFTSPINGNLSF---MDI---WLLLYDKLNPQQRDLAAFTXWAIWCDR 72

Query: 69  NNTIHNHPVPPVNVRCEWIDDYLYDFKTT--NQRIGRKAQSIQDINRLISQGKKYIMHVD 128
           N+  H   V    +RC WI  Y  ++     N+RI  +  S+   +      +   M V+
Sbjct: 73  NSXAHGSSVSTPALRCNWISSYFQNYSQAQENKRISPQQSSVPPPSCRWLPPRDSAMKVN 132

Query: 129 AAWDVVNKKSAIGVVLRDHKGQLKLAMHKGVPIFSSPLCAEVVVVFEGLKLA 172
           +     +  + +G+++RDH G L +A    +P+  +PL AE+  + E LKLA
Sbjct: 133 SDAACRSTSTGLGLIIRDHFGVLLVAKSMFLPMPLNPLFAEIRGILEALKLA 178

BLAST of Lcy06g020960 vs. NCBI nr
Match: XP_024190234.1 (uncharacterized protein LOC112194221 [Rosa chinensis])

HSP 1 Score: 75.9 bits (185), Expect = 4.8e-10
Identity = 52/182 (28.57%), Postives = 85/182 (46.70%), Query Frame = 0

Query: 6    CAWRAKEVWTRLDVGGQIGWERDMDIKDKWIHLADSVPDTVLERICVGCWAIWNDRNNTI 65
            C W +K+VW    + G     R+    D + H+  +     +E   V CW +W  RN   
Sbjct: 957  CPW-SKKVWKFSFLNGVYKQWREPSFIDLFAHVVKTSSKEEVELFSVICWLLWKSRNRFK 1016

Query: 66   H-------NHPVPPVNVRCEWIDDYLYDFKTTNQRIGRKA-QSIQDINRLISQGKKYIMH 125
            H        H V   N   EW++++  D + +N+ + + A Q    +        K  ++
Sbjct: 1017 HEGVVEESKHIVWSAN---EWLNNF-QDAQRSNENVTKDATQKRVKVKWRPPTAPKLKLN 1076

Query: 126  VDAAWDVVNKKSAIGVVLRDHKGQLKLAMHKGVPIFSSPLCAEVVVVFEGLKLARRNDVK 180
             DAA D   KK+ +G+V+RD +G+LK A HK +P   S +  E + V+ GL L R    +
Sbjct: 1077 TDAAIDYKKKKADLGMVVRDGEGKLKCAGHKVLPGNPSIVAIEALAVYHGLLLYREAGFQ 1133

BLAST of Lcy06g020960 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 75.5 bits (184), Expect = 6.3e-10
Identity = 48/180 (26.67%), Postives = 79/180 (43.89%), Query Frame = 0

Query: 9    RAKEVW-TRLDVGGQIGWERDMDIKDKWIHLADSVPDTVLERICVGCWAIWNDRNNTIHN 68
            RA+++W T       +  E ++   + W  L + +    L    +  W IWNDRN+ IH 
Sbjct: 1192 RARQIWRTLFPFLTCLSAEDNISFLELWSSLTEQLEPKDLNLAAITGWGIWNDRNSLIHG 1251

Query: 69   HPVPPVNVRCEWIDDYLYDFKTTNQRIGRKAQSIQDINRLI------SQGKKYIMHVDAA 128
              V PV  +CEW+  +L     +  ++   +   Q  +R +      S      ++ DAA
Sbjct: 1252 KQVSPVEFKCEWLTPFLDSH--SQAQMSNYSPRTQSNHRPVVQYWRPSSSVSLKLNTDAA 1311

Query: 129  WDVVNKKSAIGVVLRDHKGQLKLAMHKGVPIFSSPLCAEVVVVFEGLKLARRNDVKSLSL 182
                   ++ G ++RD    L  A    VP   SPL AE+  + EGLK A  ++   L +
Sbjct: 1312 --CRGASTSFGCIIRDSSCSLVAATSIRVPFPLSPLLAEIRCILEGLKFAAASNFTHLEV 1367

BLAST of Lcy06g020960 vs. NCBI nr
Match: XP_022141766.1 (uncharacterized protein LOC111012047 [Momordica charantia])

HSP 1 Score: 74.3 bits (181), Expect = 1.4e-09
Identity = 42/154 (27.27%), Postives = 80/154 (51.95%), Query Frame = 0

Query: 23  IGWERDMDIKDKWIHLADSVPDTVLERICVG--CWAIWNDRNNTIHNHPVPPVNVRCEWI 82
           +G + +    D+W    +++  T+ E +  G  CWA WNDRN   +   V  V+   +WI
Sbjct: 4   LGVDCNCSFIDRWALWRENL--TLEELLLAGIICWANWNDRNQRNNGIDVADVHTESDWI 63

Query: 83  DDYLYDFKTTNQRIGRKAQSIQDINRLISQGKKYI-MHVDAAWDVVNKKSAIGVVLRDHK 142
            +Y  +  T  ++     +++  +       K  + ++VDAA+DV N++  +GV++RD +
Sbjct: 64  TNYAMELLTRRRKTNEAQKNLPCLAIWKPPSKGTVKLNVDAAFDVRNQRGGVGVLVRDDR 123

Query: 143 GQLKLAMHKGVPIFSSPLCAEVVVVFEGLKLARR 174
             +  A+     + +SPL AE+  + E ++LA R
Sbjct: 124 NSILAALISSHNV-NSPLLAEICAIREAVRLAER 154

BLAST of Lcy06g020960 vs. NCBI nr
Match: XP_022145060.1 (uncharacterized protein LOC111014578 [Momordica charantia])

HSP 1 Score: 73.2 bits (178), Expect = 3.1e-09
Identity = 51/174 (29.31%), Postives = 78/174 (44.83%), Query Frame = 0

Query: 9   RAKEVWTRLDVGGQIGWERDMDIKDKWIHLADSVPDTVLERICVGCWAIWNDRNNTIHNH 68
           RAKEVW  L        + +  ++D  ++L +S+     + + VG WAIWNDRN      
Sbjct: 47  RAKEVWNILLPQAFPQCDFNNSVQDGLLYLVESLSTPDFDLVGVGVWAIWNDRNAIRMQR 106

Query: 69  PVPPVNVRCEWIDDYLYDFKTTNQRIGRKAQSIQD--INRLISQGKKYI----------- 128
            +P   +R +WI  Y+ DF+       R   S+ D  I    S   + I           
Sbjct: 107 QIPDAKIRSDWILTYVRDFQV------RDVPSLGDFRIQEDASSNDRTIENYWSPPPAGW 166

Query: 129 --MHVDAAWDVVNKKSAIGVVLRDHKGQLKLAMHKGVPIFSSPLCAEVVVVFEG 168
             ++VDAA      ++ IG+V R+ KGQ+  A          PL AE + +F+G
Sbjct: 167 IKINVDAACKKHQFRTGIGIVCRNEKGQILAAAS------HDPLMAESLALFDG 208

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1BQ492.3e-1027.91uncharacterized protein LOC111004786 OS=Momordica charantia OX=3673 GN=LOC111004... [more]
A0A6J1DX303.0e-1026.67uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024... [more]
A0A6J1CK806.8e-1027.27uncharacterized protein LOC111012047 OS=Momordica charantia OX=3673 GN=LOC111012... [more]
A0A6J1CTE31.5e-0929.31uncharacterized protein LOC111014578 OS=Momordica charantia OX=3673 GN=LOC111014... [more]
A0A7J9BAK44.4e-0929.41RNase H domain-containing protein (Fragment) OS=Gossypium gossypioides OX=34282 ... [more]
Match NameE-valueIdentityDescription
XP_022131661.14.8e-1027.91uncharacterized protein LOC111004786 [Momordica charantia][more]
XP_024190234.14.8e-1028.57uncharacterized protein LOC112194221 [Rosa chinensis][more]
XP_022158377.16.3e-1026.67uncharacterized protein LOC111024874 [Momordica charantia][more]
XP_022141766.11.4e-0927.27uncharacterized protein LOC111012047 [Momordica charantia][more]
XP_022145060.13.1e-0929.31uncharacterized protein LOC111014578 [Momordica charantia][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 118..179
e-value: 2.8E-7
score: 30.4

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy06g020960.1Lcy06g020960.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity