Lcy06g020850 (gene) Sponge gourd (P93075) v1

Overview
NameLcy06g020850
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Descriptionpurine-uracil permease NCS1
LocationChr06: 45682243 .. 45684184 (+)
RNA-Seq ExpressionLcy06g020850
SyntenyLcy06g020850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGAAATGGATGAGGACAATTCAGTAATTCTCCACCACTCACTACTCAATTCTCATCTTCTTCAAGATTCTCCTTTTCAATTTCAGTGCTCCAATCGGAATCTCCAAGGATTCCGGCCAAGTTAATGGTGACTCAATGCATTAACCTCCATCTCCACCGCCACACTTTCCACTTCCCTCCTTCTCTCTCCGCCGTCCGCCGCCGCCCCTCCGTCTCTCCGCTACGCTTCTCTCCGATCACCCCTAAATTACTTCTCAAAATCCGCTCTAACCCTAACCCTAACTTCACCCCAATGGCCTCCGCCGCCGACCGCTCGACGGAGGATCCTCACCTCGTGGATTCCGGCAGCGAAGATCTCCGGCCGACGACGCTGGAGCAGAGAACGCTCTCCTGGCCGGAAATGGCTCTGCTATGGATCGGCCTCGTCGTCGGTGTCCCGGCTTATTACCTCGCCGGAAGCTTAGTCGATCTCGGAATGGCGTGGTGGCAGGGCATCGCCACCGTCGTCGCCGCCAACGCCGTAACCCTAATTGCCCTGATTCTCACCGGCCATCCCGGCACGAAATACGGCATCTCCTTCCCTGTCTTTGCCCGATCGTCGTTCGGAATCCACGGCGCTCACATCCCCACTCTTCTCCGAGCTCTGATCGGCTGCGGTTGGTACGGAATCGAAACGTGGATCGCCGGTGAGGCCATTTTCCTTCTCTTGCCTGAAAGTATCAAGAAATCTTCATTCTCACAGGTTCTTCCATGGCTGGGAACTTCCCCTCTTGCTTTTGCTTGCTTTTTAGCCTTCTGGGTCGTCCAATTAGCCAGTGTTTGGAAAGGAATGGATGGAATTCGAAAACTCGAAACCTTCTCTGCTCCGATTCTCGTTTTTCTCACGATTGCTCTGTTGATTTGGTCTTGTGTTCGAGCCGGTGGATTCGGGCATATGCTTTCTTTAACCTCGAAACTCTCTTCCTCTGAATTTTGGGCTCTGTTTTCCCCTTCTCTTACTGCTAATATAAGCTTCTGGGCCACTCTGGCTTTGAATATCCCTGATTTCACTCGCTATGCCAGAAGTCAAAAGGATCAAATCATTGGCCAATTGGGGCTTCCCATTTTCATGGGGCTGTTCACTTTCGTCGGCGTTGCTGTCACTTCCTCCACCGGAGTGATCTTCGGGCATGTAATTTCGAACCCAATTGATCTTCTCGGCCATATTGGGGGTTTTGCGACTAAGGTTTTGGCCATTTTTGGGATCATTTTGGCGACTGTGACCACCAATGTCGCCGCCAATGTGGTGGCGCCGGCCAATGCGTTGGTGAATCTCAGCCCTTCTTATTTCACTTTCACCAGAGGGGCGCTTCTCACCGCCGTGATCGGCATTGTTTTTCAGCCGTGGAGGCTTCTGAGCTCGAGTGAGAGCTTCGTTTACACTTGGCTTGTTGGGTATTCGGCGCTGTTGGGGCCGATCGGCGGCGTGATGGTGACGGATTACTATTTGATTCGGCGTAGGGAGCTGAGCGTTGAGGACTTGTATTCTTTGAGTCCGACGGGGGCTTATTACTACTCCGGTGGGTTTAATTTGGCGGCGGTGGCGGCGCTGGTGGTCGGAGTTTTGCCGGTGATTCCTGGGTTCTTGGAGAAGGTGGAGATCGTGTCGAAAGTTCCGAAAGTATTTAGTGTCATTTACGGGAATGCTTGGTTTATTAGTAGCTTTTTGGCTGGATTTTGCTATTGGAGTCTCTCTGTTTTGTTCAAACGGCGAAAGGTTTCTACTTCTAGCCGTTTTGGGCCACTGCTTTGAACTTTAGAAAATCTCGCCAAATTGAGTATAGCTCGGTGTTATGATCACGACCAAAATGTTAAAAGTTTAAACCATACAACATGTATTGTCGAACATAATAAAGAAGAAAATGTTAGAAGTTCAAACCACTCCCATGTATTTTTTTAACTC

mRNA sequence

TGGAAATGGATGAGGACAATTCAGTAATTCTCCACCACTCACTACTCAATTCTCATCTTCTTCAAGATTCTCCTTTTCAATTTCAGTGCTCCAATCGGAATCTCCAAGGATTCCGGCCAAGTTAATGGTGACTCAATGCATTAACCTCCATCTCCACCGCCACACTTTCCACTTCCCTCCTTCTCTCTCCGCCGTCCGCCGCCGCCCCTCCGTCTCTCCGCTACGCTTCTCTCCGATCACCCCTAAATTACTTCTCAAAATCCGCTCTAACCCTAACCCTAACTTCACCCCAATGGCCTCCGCCGCCGACCGCTCGACGGAGGATCCTCACCTCGTGGATTCCGGCAGCGAAGATCTCCGGCCGACGACGCTGGAGCAGAGAACGCTCTCCTGGCCGGAAATGGCTCTGCTATGGATCGGCCTCGTCGTCGGTGTCCCGGCTTATTACCTCGCCGGAAGCTTAGTCGATCTCGGAATGGCGTGGTGGCAGGGCATCGCCACCGTCGTCGCCGCCAACGCCGTAACCCTAATTGCCCTGATTCTCACCGGCCATCCCGGCACGAAATACGGCATCTCCTTCCCTGTCTTTGCCCGATCGTCGTTCGGAATCCACGGCGCTCACATCCCCACTCTTCTCCGAGCTCTGATCGGCTGCGGTTGGTACGGAATCGAAACGTGGATCGCCGGTGAGGCCATTTTCCTTCTCTTGCCTGAAAGTATCAAGAAATCTTCATTCTCACAGGTTCTTCCATGGCTGGGAACTTCCCCTCTTGCTTTTGCTTGCTTTTTAGCCTTCTGGGTCGTCCAATTAGCCAGTGTTTGGAAAGGAATGGATGGAATTCGAAAACTCGAAACCTTCTCTGCTCCGATTCTCGTTTTTCTCACGATTGCTCTGTTGATTTGGTCTTGTGTTCGAGCCGGTGGATTCGGGCATATGCTTTCTTTAACCTCGAAACTCTCTTCCTCTGAATTTTGGGCTCTGTTTTCCCCTTCTCTTACTGCTAATATAAGCTTCTGGGCCACTCTGGCTTTGAATATCCCTGATTTCACTCGCTATGCCAGAAGTCAAAAGGATCAAATCATTGGCCAATTGGGGCTTCCCATTTTCATGGGGCTGTTCACTTTCGTCGGCGTTGCTGTCACTTCCTCCACCGGAGTGATCTTCGGGCATGTAATTTCGAACCCAATTGATCTTCTCGGCCATATTGGGGGTTTTGCGACTAAGGTTTTGGCCATTTTTGGGATCATTTTGGCGACTGTGACCACCAATGTCGCCGCCAATGTGGTGGCGCCGGCCAATGCGTTGGTGAATCTCAGCCCTTCTTATTTCACTTTCACCAGAGGGGCGCTTCTCACCGCCGTGATCGGCATTGTTTTTCAGCCGTGGAGGCTTCTGAGCTCGAGTGAGAGCTTCGTTTACACTTGGCTTGTTGGGTATTCGGCGCTGTTGGGGCCGATCGGCGGCGTGATGGTGACGGATTACTATTTGATTCGGCGTAGGGAGCTGAGCGTTGAGGACTTGTATTCTTTGAGTCCGACGGGGGCTTATTACTACTCCGGTGGGTTTAATTTGGCGGCGGTGGCGGCGCTGGTGGTCGGAGTTTTGCCGGTGATTCCTGGGTTCTTGGAGAAGGTGGAGATCGTGTCGAAAGTTCCGAAAGTATTTAGTGTCATTTACGGGAATGCTTGGTTTATTAGTAGCTTTTTGGCTGGATTTTGCTATTGGAGTCTCTCTGTTTTGTTCAAACGGCGAAAGGTTTCTACTTCTAGCCGTTTTGGGCCACTGCTTTGAACTTTAGAAAATCTCGCCAAATTGAGTATAGCTCGGTGTTATGATCACGACCAAAATGTTAAAAGTTTAAACCATACAACATGTATTGTCGAACATAATAAAGAAGAAAATGTTAGAAGTTCAAACCACTCCCATGTATTTTTTTAACTC

Coding sequence (CDS)

ATGGTGACTCAATGCATTAACCTCCATCTCCACCGCCACACTTTCCACTTCCCTCCTTCTCTCTCCGCCGTCCGCCGCCGCCCCTCCGTCTCTCCGCTACGCTTCTCTCCGATCACCCCTAAATTACTTCTCAAAATCCGCTCTAACCCTAACCCTAACTTCACCCCAATGGCCTCCGCCGCCGACCGCTCGACGGAGGATCCTCACCTCGTGGATTCCGGCAGCGAAGATCTCCGGCCGACGACGCTGGAGCAGAGAACGCTCTCCTGGCCGGAAATGGCTCTGCTATGGATCGGCCTCGTCGTCGGTGTCCCGGCTTATTACCTCGCCGGAAGCTTAGTCGATCTCGGAATGGCGTGGTGGCAGGGCATCGCCACCGTCGTCGCCGCCAACGCCGTAACCCTAATTGCCCTGATTCTCACCGGCCATCCCGGCACGAAATACGGCATCTCCTTCCCTGTCTTTGCCCGATCGTCGTTCGGAATCCACGGCGCTCACATCCCCACTCTTCTCCGAGCTCTGATCGGCTGCGGTTGGTACGGAATCGAAACGTGGATCGCCGGTGAGGCCATTTTCCTTCTCTTGCCTGAAAGTATCAAGAAATCTTCATTCTCACAGGTTCTTCCATGGCTGGGAACTTCCCCTCTTGCTTTTGCTTGCTTTTTAGCCTTCTGGGTCGTCCAATTAGCCAGTGTTTGGAAAGGAATGGATGGAATTCGAAAACTCGAAACCTTCTCTGCTCCGATTCTCGTTTTTCTCACGATTGCTCTGTTGATTTGGTCTTGTGTTCGAGCCGGTGGATTCGGGCATATGCTTTCTTTAACCTCGAAACTCTCTTCCTCTGAATTTTGGGCTCTGTTTTCCCCTTCTCTTACTGCTAATATAAGCTTCTGGGCCACTCTGGCTTTGAATATCCCTGATTTCACTCGCTATGCCAGAAGTCAAAAGGATCAAATCATTGGCCAATTGGGGCTTCCCATTTTCATGGGGCTGTTCACTTTCGTCGGCGTTGCTGTCACTTCCTCCACCGGAGTGATCTTCGGGCATGTAATTTCGAACCCAATTGATCTTCTCGGCCATATTGGGGGTTTTGCGACTAAGGTTTTGGCCATTTTTGGGATCATTTTGGCGACTGTGACCACCAATGTCGCCGCCAATGTGGTGGCGCCGGCCAATGCGTTGGTGAATCTCAGCCCTTCTTATTTCACTTTCACCAGAGGGGCGCTTCTCACCGCCGTGATCGGCATTGTTTTTCAGCCGTGGAGGCTTCTGAGCTCGAGTGAGAGCTTCGTTTACACTTGGCTTGTTGGGTATTCGGCGCTGTTGGGGCCGATCGGCGGCGTGATGGTGACGGATTACTATTTGATTCGGCGTAGGGAGCTGAGCGTTGAGGACTTGTATTCTTTGAGTCCGACGGGGGCTTATTACTACTCCGGTGGGTTTAATTTGGCGGCGGTGGCGGCGCTGGTGGTCGGAGTTTTGCCGGTGATTCCTGGGTTCTTGGAGAAGGTGGAGATCGTGTCGAAAGTTCCGAAAGTATTTAGTGTCATTTACGGGAATGCTTGGTTTATTAGTAGCTTTTTGGCTGGATTTTGCTATTGGAGTCTCTCTGTTTTGTTCAAACGGCGAAAGGTTTCTACTTCTAGCCGTTTTGGGCCACTGCTTTGA

Protein sequence

MVTQCINLHLHRHTFHFPPSLSAVRRRPSVSPLRFSPITPKLLLKIRSNPNPNFTPMASAADRSTEDPHLVDSGSEDLRPTTLEQRTLSWPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETWIAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFACFLAFWVVQLASVWKGMDGIRKLETFSAPILVFLTIALLIWSCVRAGGFGHMLSLTSKLSSSEFWALFSPSLTANISFWATLALNIPDFTRYARSQKDQIIGQLGLPIFMGLFTFVGVAVTSSTGVIFGHVISNPIDLLGHIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSLSPTGAYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGNAWFISSFLAGFCYWSLSVLFKRRKVSTSSRFGPLL
Homology
BLAST of Lcy06g020850 vs. ExPASy Swiss-Prot
Match: Q9LZD0 (Purine-uracil permease NCS1 OS=Arabidopsis thaliana OX=3702 GN=NCS1 PE=1 SV=1)

HSP 1 Score: 711.1 bits (1834), Expect = 1.0e-203
Identity = 359/529 (67.86%), Postives = 421/529 (79.58%), Query Frame = 0

Query: 35  FSPITP--KLLLKIRSNPNPNFTPMASAA------DRSTEDPHLVDSGSEDLRPTTLEQR 94
           FSPI P   L+L       P  + M  +       D S  DP L    ++DL+PTT  QR
Sbjct: 74  FSPILPGDNLVLSRSGVIRPRLSAMTGSEINDHGYDESQFDPSLT---NDDLKPTTPSQR 133

Query: 95  TLSWPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQGIATVVAANAVTLIALILTGHPGT 154
           T SW +M+ LWIGLVVGVP YYLAGSLVDLGMAWWQGIATVV AN + L+ L+LT  PGT
Sbjct: 134 TFSWLDMSSLWIGLVVGVPTYYLAGSLVDLGMAWWQGIATVVTANLILLVPLVLTAQPGT 193

Query: 155 KYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETWIAGEAIFLLLPESIKKSSFSQ 214
            YGISFPV ARSSFGI GAHIPTLLRAL+GCGWYGIETWI GEAIFLLLP  IKKS+ S 
Sbjct: 194 LYGISFPVLARSSFGIRGAHIPTLLRALVGCGWYGIETWIGGEAIFLLLPGHIKKSALSH 253

Query: 215 VLPWLGTSPLAFACFLAFWVVQLASVWKGMDGIRKLETFSAPILVFLTIALLIWSCVRAG 274
            LPWLGTSPL F+CF+ FW+ QL  VW+GMDGIRKLE +SAPIL+ LT  LL WS ++AG
Sbjct: 254 TLPWLGTSPLEFSCFIVFWLAQLCIVWRGMDGIRKLEKYSAPILISLTSCLLAWSYLKAG 313

Query: 275 GFGHMLSLTSKLSSSEFWALFSPSLTANISFWATLALNIPDFTRYARSQKDQIIGQLGLP 334
           GFGHMLSL+SKL+S++FW LF PSLTANISFWATLALNIPDF+R+A+SQ DQIIGQ+GLP
Sbjct: 314 GFGHMLSLSSKLTSAQFWTLFFPSLTANISFWATLALNIPDFSRFAKSQTDQIIGQVGLP 373

Query: 335 IFMGLFTFVGVAVTSSTGVIFGHVISNPIDLLGHIGGFATKVLAIFGIILATVTTNVAAN 394
           +FMGLFTFVGVAVTSST +IFG VISNPI+LLG IGG AT +LAI GI LAT+TTN+AAN
Sbjct: 374 VFMGLFTFVGVAVTSSTSIIFGRVISNPIELLGQIGGLATTLLAIVGISLATLTTNIAAN 433

Query: 395 VVAPANALVNLSPSYFTFTRGALLTAVIGIVFQPWRLLSSSESFVYTWLVGYSALLGPIG 454
           VVAPANALVNL+P +FTF RGA LTAV+GIVFQPWRLL SSESFVYTWL+GYSALLGPIG
Sbjct: 434 VVAPANALVNLNPKFFTFGRGAFLTAVLGIVFQPWRLLKSSESFVYTWLIGYSALLGPIG 493

Query: 455 GVMVTDYYLIRRRELSVEDLYSLSPTGAYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEI 514
           G+++ DYYLI++ +L++ DLYSLSP+G YY+S G+N+AAV ALV G++PV+PGFL K+  
Sbjct: 494 GIILVDYYLIKKMKLNIGDLYSLSPSGEYYFSKGYNVAAVVALVAGIIPVVPGFLHKISA 553

Query: 515 VSKVPKVFSVIYGNAWFISSFLAGFCYWSLSVLFKRRKVSTSSRFGPLL 556
           +SK+   F V+Y NA F S  +AGF YW +     R++ S SS   PLL
Sbjct: 554 LSKISNGFVVVYDNALFFSFIIAGFVYWIIMSRLGRKQSSLSSSSHPLL 599

BLAST of Lcy06g020850 vs. ExPASy Swiss-Prot
Match: P94575 (Probable allantoin permease OS=Bacillus subtilis (strain 168) OX=224308 GN=pucI PE=2 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.7e-78
Identity = 173/463 (37.37%), Postives = 257/463 (55.51%), Query Frame = 0

Query: 75  SEDLRPTTLEQRTLSWPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQGIATVVAANAVT 134
           +EDL P   E+RT      A +W+G +  +P Y   G L+ +G++ WQ +A ++ A+ + 
Sbjct: 15  NEDLVPLGQEKRTWKAMNFASIWMGCIHNIPTYATVGGLIAIGLSPWQVLAIIITASLIL 74

Query: 135 LIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETWIAGEAI-FL 194
             AL L GH GTKYG+ FPV  R+S+GI+GA+IP LLRA     W GI+T+    A+  L
Sbjct: 75  FGALALNGHAGTKYGLPFPVIIRASYGIYGANIPALLRAFTAIMWLGIQTFAGSTALNIL 134

Query: 195 LLPESIKKSSFSQVLPWLGTSPLAFACFLAFWVVQLASVWKGMDGIRKLETFSAPILVFL 254
           LL               LG        F+ FW + L  +  GM+ I++ E ++ P++  +
Sbjct: 135 LLNMWPGWGEIGGEWNILGIHLSGLLSFVFFWAIHLLVLHHGMESIKRFEVWAGPLVYLV 194

Query: 255 TIALLIWSCVRAGGFGHMLSLTSKLS--SSEFWALFSPSLTANISFWATLALNIPDFTRY 314
              ++ W+   AGG G + S   K    S  FW  F+  +T  I  WATL LNIPDFTR+
Sbjct: 195 FGGMVWWAVDIAGGLGPIYSQPGKFHTFSETFWP-FAAGVTGIIGIWATLILNIPDFTRF 254

Query: 315 ARSQKDQIIGQL-GLPIFMGLFTFVGVAVTSSTGVIFGHVISNPIDLLGHIGGFATKVLA 374
           A +QK+QI GQ  GLP    LF F  + VTS + V FG  I + +D+L         VL+
Sbjct: 255 AETQKEQIKGQFYGLPGTFALFAFASITVTSGSQVAFGEPIWDVVDILARFDNPYVIVLS 314

Query: 375 IFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIVFQPWRLLSSSESF 434
           +  + +AT++ NVAAN+V+PA  + N  P Y  F RG+ +TA++ +   PW+L+ S+ S 
Sbjct: 315 VITLCIATISVNVAANIVSPAYDIANALPKYINFKRGSFITALLALFTVPWKLMESATS- 374

Query: 435 VYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSLSPTGAYYYSGGFNLAAVAALV 494
           VY +L     +LGP+ GVM+ DY++IR+RELSV+DLY  S TG Y Y  G+N  A AA +
Sbjct: 375 VYAFLGLIGGMLGPVAGVMMADYFIIRKRELSVDDLY--SETGRYVYWKGYNYRAFAATM 434

Query: 495 VGVLPVIPGFLEKVEIVSKVPKVFSVIYGNAWFISSFLAGFCY 534
           +G L         + ++     V   +Y  +WF+   ++   Y
Sbjct: 435 LGAL---------ISLIGMYVPVLKSLYDISWFVGVLISFLFY 464

BLAST of Lcy06g020850 vs. ExPASy Swiss-Prot
Match: O14035 (Uncharacterized permease C29B12.14c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC29B12.14c PE=3 SV=2)

HSP 1 Score: 239.6 bits (610), Expect = 8.6e-62
Identity = 161/470 (34.26%), Postives = 247/470 (52.55%), Query Frame = 0

Query: 75  SEDLRPTTLEQRTLSWPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQGIATVVAANAVT 134
           +EDL P    QR          W+   V V  + +AG+ V+ G++WW+   TV     + 
Sbjct: 50  NEDLYPVPKSQRVWGPWNYVAFWLADSVNVNTWMIAGTAVESGLSWWEAWITVWVGYTIA 109

Query: 135 LIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETWIAGEAIFLL 194
              L + G  G  Y ISFPV +RSSFGI G+  P L RA++ C WYG++ WI GE + L+
Sbjct: 110 AFILTIAGRAGAVYHISFPVLSRSSFGIWGSLWPILNRAVMACVWYGVQAWIGGECVTLM 169

Query: 195 LPESIKKSSFSQV---LPWLGTSPLAFACFLAFWVVQLASVWKGMDGIRKLETFSAPILV 254
           +  SI   SFS +   +   GT    +  F  FW++   ++W  +  IR L T  + +  
Sbjct: 170 I-RSI-WPSFSHIPNTMAKSGTETYQWVGFFIFWLISNVAIWFPVYQIRHLFTAKSFLAP 229

Query: 255 FLTIALLIWSCVRAGGFGHMLSLTSKLSS-SEFWALFSPSLTANISFWATLALNIPDFTR 314
              IA LIW+ V+A G G  +   ++LS+ +  WA+ +  + + +  +ATL +N PDFTR
Sbjct: 230 PAAIAFLIWALVKAHGAGDAIHAKTQLSTWNHGWAV-TAGIISCLDNFATLIVNNPDFTR 289

Query: 315 YARSQKDQIIGQL-GLPIFMGLFTFVGVAVTSSTGVIFGHVISNPIDLL-------GHIG 374
           +A +    I  QL  +P+  G+ T +GV V S++  I+G  I NP+DLL        H G
Sbjct: 290 FATTPNAPIFPQLITIPMGFGITTLIGVLVGSASKSIYGENIWNPLDLLKSFLDHSNHHG 349

Query: 375 GFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIVFQPWR 434
             A       G+ LA +  N+AAN V+  N    L P +    RG  + ++IGI   PW 
Sbjct: 350 VRAGVFFISTGLCLAQLGVNIAANTVSAGNDTSALCPMFINIRRGGYIASIIGICMCPWN 409

Query: 435 LLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSLSPTGAYYYSGGFN 494
           LLSSS SF  + L  Y+  L    G+++ DY++IR+  L V+ LY+++P   Y+++ G N
Sbjct: 410 LLSSSNSFANS-LSAYAVFLSSFAGILIADYFVIRKGYLKVDALYTINPNEPYWFTYGIN 469

Query: 495 LAAVAALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGNAW---FISSFLA 530
           L A A+ + G+L  + G    V    KVPK    +   A+    ++SFL+
Sbjct: 470 LRAFASYICGLLINVVGLAGAVG--DKVPKAALTMNNIAYLLGIVTSFLS 513

BLAST of Lcy06g020850 vs. ExPASy Swiss-Prot
Match: Q10279 (Uracil permease OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=fur4 PE=1 SV=2)

HSP 1 Score: 221.5 bits (563), Expect = 2.4e-56
Identity = 150/467 (32.12%), Postives = 237/467 (50.75%), Query Frame = 0

Query: 75  SEDLRPTTLEQRTLSWPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQGIATVVAANAVT 134
           ++DL P    +R          W+   V +  + ++ + ++LG+ WW+    V     + 
Sbjct: 43  NKDLYPVPPSKRLWGPWNFISFWLADAVNINTWMISATAIELGLNWWEAWICVWVGYLIC 102

Query: 135 LIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETWIAGEAIFLL 194
            I +  TG PG  Y ISFPV +RSSFG  G+  P L R+++ C WYG++ WI GE + L+
Sbjct: 103 GILVATTGRPGAVYHISFPVLSRSSFGTWGSLWPILNRSVLACVWYGVQAWIGGECVVLM 162

Query: 195 LPESIKKSSFSQV---LPWLGTSPLAFACFLAFWVVQLASVWKGMDGIRKLETFSAPILV 254
           +  SI   SFS +   +   GT    +  F  FW++   ++W  +  IR L TF A +  
Sbjct: 163 I-RSI-WPSFSHIPNTMAKSGTETYQWVGFFIFWLLSNIAIWFPVHQIRHLFTFKAIVAP 222

Query: 255 FLTIALLIWSCVRAGGFGHMLSLTSKLSSSEF-WALFSPSLTANISFWATLALNIPDFTR 314
              IA LIW+ V+A G G ++   +KL   E  W + +  +T  I  +ATL +N PDF R
Sbjct: 223 PAAIAFLIWALVKAHGAGPVIHEPTKLGQYEHAWVVINGIVTC-IDGFATLIVNNPDFAR 282

Query: 315 YARSQKDQIIGQ-LGLPIFMGLFTFVGVAVTSSTGVIFGHVISNPIDLLGHI-------G 374
           +A +       Q + +P+  G+ + +GV V+S++  I+G  + +P  LL          G
Sbjct: 283 FATTPGAVHWPQIITVPLAFGVTSLIGVLVSSASKAIYGTTLWDPTQLLASFLDHSNAHG 342

Query: 375 GFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIVFQPWR 434
             A      FG+ +A +  N+AAN V+  N L  L P+     RG  + ++I +   PW 
Sbjct: 343 VRAGVFFIAFGLCIAQLGVNIAANSVSAGNDLSALLPTVINVRRGGYIASIIALCMCPWN 402

Query: 435 LLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSLSPTGAYYYSGGFN 494
           LLSS+ +F  T+L  YS  L    GV++ DYY +R+  + V  LYS S +  YY+  G N
Sbjct: 403 LLSSNNNFT-TYLSSYSVFLSSFAGVIIADYYFVRKGLIRVAPLYSSSSSSPYYFWKGIN 462

Query: 495 LAAVAALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGNAWFISSFLA 530
             A A+ + G+L  I G         KVPKV + ++   +F+   +A
Sbjct: 463 FRAFASYICGMLINIVGMAGSTG--QKVPKVANTMFNLNYFLGITVA 503

BLAST of Lcy06g020850 vs. ExPASy Swiss-Prot
Match: Q04895 (Allantoin permease OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DAL4 PE=1 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.9e-48
Identity = 140/489 (28.63%), Postives = 239/489 (48.88%), Query Frame = 0

Query: 62  DRSTEDPHLVDS--GSEDLRPTTLEQRTLSWPEMALLWIGLVVGVPAYYLAGSLVDLGMA 121
           D++T +  L +S   + DL+P   E+R  SW      W+     +  + +AG+ + LG+ 
Sbjct: 85  DKTTLNVSLKESFLYNRDLKPVEEERRCWSWFNYLYFWLADCFNINTWQIAGTGLQLGLN 144

Query: 122 WWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGW 181
           WWQ   TV        I ++L    G+ Y +SFP+  R+SFGI  +  P + R ++   W
Sbjct: 145 WWQCWLTVWIGYTFAGIFVVLNSRFGSAYHLSFPITVRASFGIFFSMWPIINRVVMAIVW 204

Query: 182 YGIETWIAGEAIFLLLPESIKKSSFSQVLPWLG---TSPLAFACFLAFWVVQLASVWKGM 241
           Y ++ W+    + L+L     K+   ++    G   ++   F CF  FWVV +  V    
Sbjct: 205 YAVQAWLGATPVALMLKSIFGKNLEDRIPNHFGSPNSTTFEFMCFFIFWVVSIPFVLVAP 264

Query: 242 DGIRKLETFSAPILVFLTIALLIWSCVRAGGFGHMLSLTS-KLSSSEFWALFSPSLTANI 301
             IR L T  A ++ F     LIW+  ++ G   + +L       SEF  +F  SL A +
Sbjct: 265 HKIRHLFTVKAALIPFAAFGFLIWALKKSHGKIELGTLNDYSPHGSEFSWIFVRSLMACV 324

Query: 302 SFWATLALNIPDFTRYARSQKDQIIGQL-GLPIFMGLFTFVGVAVTSSTGVIFGHVISNP 361
           + +A L +N PDF R+A++ +  +  QL  +P+F  +   +G+ VT++   ++G    +P
Sbjct: 325 ANFAALIINAPDFGRFAKNPQASLWPQLVAIPLFFAITCLIGIIVTAAGYHLYGVNYWSP 384

Query: 362 IDLLGHI-------GGFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRG 421
           +D+LG         G  A   L  F   LA + TN++AN +A    +  L P Y    RG
Sbjct: 385 LDVLGQFLETTYTRGTRAGVFLISFVFALAQLGTNISANSLACGADMTALFPRYINIRRG 444

Query: 422 ALLTAVIGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLY 481
           +L    + +   PW L++SS  F  + L  Y+  L  I GV+  DY+++RR  + +  L+
Sbjct: 445 SLFCVAMALCICPWNLMASSSKFT-SALGAYAIFLSSIAGVICADYFVVRRGYVKLTHLF 504

Query: 482 SLSPTGAYYYSG---GFNLAAVAALVVGVLPVIPGFL-----EKVEIVSKVPKVFSVIYG 529
            L+  G++Y  G   G N  A  A + G+ P +PGF+      K+ +     +++ + Y 
Sbjct: 505 -LAQKGSFYMFGNKFGANWRAFVAYICGIAPNLPGFIGDVGAPKITVSEGAMRLYYLGYP 564

BLAST of Lcy06g020850 vs. ExPASy TrEMBL
Match: A0A5D3DPI8 (Purine-uracil permease NCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G003170 PE=3 SV=1)

HSP 1 Score: 973.0 bits (2514), Expect = 5.2e-280
Identity = 501/558 (89.78%), Postives = 517/558 (92.65%), Query Frame = 0

Query: 1   MVTQCINLHLHRHTFHFPPSLSAVRRRPSV--SPLRFSPITPKLLLKIRSNPNPNFT-PM 60
           MVT CINLHLHRH FH PPS+S +R RP+V  SPL FSPITPKLLLK RS  N  FT PM
Sbjct: 1   MVTHCINLHLHRHIFHLPPSISTLRYRPTVSHSPLLFSPITPKLLLKRRS--NLRFTSPM 60

Query: 61  ASAADRSTEDPHLVDSGSEDLRPTTLEQRTLSWPEMALLWIGLVVGVPAYYLAGSLVDLG 120
           ASA    +ED    DSG +DLRPTTLEQRTLSWPEMALLWIGL+VGVP+YYLAGSLVDLG
Sbjct: 61  ASADANPSEDTQNDDSGKDDLRPTTLEQRTLSWPEMALLWIGLIVGVPSYYLAGSLVDLG 120

Query: 121 MAWWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGC 180
           M+WWQGIATVV ANAVTLIALIL GHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGC
Sbjct: 121 MSWWQGIATVVVANAVTLIALILNGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGC 180

Query: 181 GWYGIETWIAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFACFLAFWVVQLASVWKGMD 240
           GWYGIETWIAGEAIFLLLPESIKKSSFSQ+LPWLGTSPLAF CFLAFWVVQLASVWKGMD
Sbjct: 181 GWYGIETWIAGEAIFLLLPESIKKSSFSQILPWLGTSPLAFTCFLAFWVVQLASVWKGMD 240

Query: 241 GIRKLETFSAPILVFLTIALLIWSCVRAGGFGHMLSLTSKLSSSEFWALFSPSLTANISF 300
           GIRKLETFSAPILVFLT+ LLIWSCV+AGGFGHMLSLTSKLSSSEFWALF PSLTANISF
Sbjct: 241 GIRKLETFSAPILVFLTVCLLIWSCVKAGGFGHMLSLTSKLSSSEFWALFFPSLTANISF 300

Query: 301 WATLALNIPDFTRYARSQKDQIIGQLGLPIFMGLFTFVGVAVTSSTGVIFGHVISNPIDL 360
           WATLALNIPDFTRYA+SQKDQIIGQLGLPIFMG FTFVGVAVTSSTGVIFGH+ISNPIDL
Sbjct: 301 WATLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIDL 360

Query: 361 LGHIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIV 420
           LGHIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGI+
Sbjct: 361 LGHIGGFVTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIL 420

Query: 421 FQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSLSPTGAYYY 480
           FQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIR +ELSVEDLYS SP GAYYY
Sbjct: 421 FQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRGKELSVEDLYSTSPRGAYYY 480

Query: 481 SGGFNLAAVAALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGNAWFISSFLAGFCYWSLS 540
           SGGFNL AV ALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYG+AWFISSFLAGFCYW LS
Sbjct: 481 SGGFNLVAVVALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGSAWFISSFLAGFCYWGLS 540

Query: 541 VLFKRRKVSTSSRFGPLL 556
           VL KRRK S   RFGPLL
Sbjct: 541 VLLKRRKASL--RFGPLL 554

BLAST of Lcy06g020850 vs. ExPASy TrEMBL
Match: A0A1S3B756 (purine-uracil permease NCS1 OS=Cucumis melo OX=3656 GN=LOC103486539 PE=3 SV=1)

HSP 1 Score: 973.0 bits (2514), Expect = 5.2e-280
Identity = 501/558 (89.78%), Postives = 517/558 (92.65%), Query Frame = 0

Query: 1   MVTQCINLHLHRHTFHFPPSLSAVRRRPSV--SPLRFSPITPKLLLKIRSNPNPNFT-PM 60
           MVT CINLHLHRH FH PPS+S +R RP+V  SPL FSPITPKLLLK RS  N  FT PM
Sbjct: 1   MVTHCINLHLHRHIFHLPPSISTLRYRPTVSHSPLLFSPITPKLLLKRRS--NLRFTSPM 60

Query: 61  ASAADRSTEDPHLVDSGSEDLRPTTLEQRTLSWPEMALLWIGLVVGVPAYYLAGSLVDLG 120
           ASA    +ED    DSG +DLRPTTLEQRTLSWPEMALLWIGL+VGVP+YYLAGSLVDLG
Sbjct: 61  ASADANPSEDTQNDDSGKDDLRPTTLEQRTLSWPEMALLWIGLIVGVPSYYLAGSLVDLG 120

Query: 121 MAWWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGC 180
           M+WWQGIATVV ANAVTLIALIL GHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGC
Sbjct: 121 MSWWQGIATVVVANAVTLIALILNGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGC 180

Query: 181 GWYGIETWIAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFACFLAFWVVQLASVWKGMD 240
           GWYGIETWIAGEAIFLLLPESIKKSSFSQ+LPWLGTSPLAF CFLAFWVVQLASVWKGMD
Sbjct: 181 GWYGIETWIAGEAIFLLLPESIKKSSFSQILPWLGTSPLAFTCFLAFWVVQLASVWKGMD 240

Query: 241 GIRKLETFSAPILVFLTIALLIWSCVRAGGFGHMLSLTSKLSSSEFWALFSPSLTANISF 300
           GIRKLETFSAPILVFLT+ LLIWSCV+AGGFGHMLSLTSKLSSSEFWALF PSLTANISF
Sbjct: 241 GIRKLETFSAPILVFLTVCLLIWSCVKAGGFGHMLSLTSKLSSSEFWALFFPSLTANISF 300

Query: 301 WATLALNIPDFTRYARSQKDQIIGQLGLPIFMGLFTFVGVAVTSSTGVIFGHVISNPIDL 360
           WATLALNIPDFTRYA+SQKDQIIGQLGLPIFMG FTFVGVAVTSSTGVIFGH+ISNPIDL
Sbjct: 301 WATLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIDL 360

Query: 361 LGHIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIV 420
           LGHIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGI+
Sbjct: 361 LGHIGGFVTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIL 420

Query: 421 FQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSLSPTGAYYY 480
           FQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIR +ELSVEDLYS SP GAYYY
Sbjct: 421 FQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRGKELSVEDLYSTSPRGAYYY 480

Query: 481 SGGFNLAAVAALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGNAWFISSFLAGFCYWSLS 540
           SGGFNL AV ALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYG+AWFISSFLAGFCYW LS
Sbjct: 481 SGGFNLVAVVALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGSAWFISSFLAGFCYWGLS 540

Query: 541 VLFKRRKVSTSSRFGPLL 556
           VL KRRK S   RFGPLL
Sbjct: 541 VLLKRRKASL--RFGPLL 554

BLAST of Lcy06g020850 vs. ExPASy TrEMBL
Match: A0A6J1KWR9 (purine-uracil permease NCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498292 PE=3 SV=1)

HSP 1 Score: 968.8 bits (2503), Expect = 9.9e-279
Identity = 492/550 (89.45%), Postives = 513/550 (93.27%), Query Frame = 0

Query: 6   INLHLHRHTFHFPPSLSAVRRRPSVSPLRFSPITPKLLLKIRSNPNPNFTPMASAADRST 65
           +NLHLHRH F FPP L+ VRR P+VS LRFSPITPKLLL+ R   N NF PMASAA+  T
Sbjct: 18  VNLHLHRHNFRFPPCLTTVRRHPTVSLLRFSPITPKLLLRHRFKSNTNFIPMASAANNPT 77

Query: 66  EDPHLVDSGSEDLRPTTLEQRTLSWPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQGIA 125
           ++ H+ + GS+DLRPTT EQRTL+WPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQGIA
Sbjct: 78  DNTHVENPGSDDLRPTTPEQRTLAWPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQGIA 137

Query: 126 TVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETW 185
           TVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETW
Sbjct: 138 TVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETW 197

Query: 186 IAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFACFLAFWVVQLASVWKGMDGIRKLETF 245
           IAGEAIFLLLPESIK SSFSQVLPWLGTSPLAF CFLAFWVVQLASVWKGMDGIRKLET 
Sbjct: 198 IAGEAIFLLLPESIKNSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGIRKLETI 257

Query: 246 SAPILVFLTIALLIWSCVRAGGFGHMLSLTSKLSSSEFWALFSPSLTANISFWATLALNI 305
           SAPILVFLT+ LLIWSCVRAGGFGHMLSL SKLSSSEFWALF PSLTANISFWATLALNI
Sbjct: 258 SAPILVFLTMGLLIWSCVRAGGFGHMLSLNSKLSSSEFWALFFPSLTANISFWATLALNI 317

Query: 306 PDFTRYARSQKDQIIGQLGLPIFMGLFTFVGVAVTSSTGVIFGHVISNPIDLLGHIGGFA 365
           PDFTRYA+SQKDQIIGQLGLP+FMG FTFVGVAVTSSTGVIFGH+ISNPIDLLGHIGGFA
Sbjct: 318 PDFTRYAKSQKDQIIGQLGLPVFMGAFTFVGVAVTSSTGVIFGHIISNPIDLLGHIGGFA 377

Query: 366 TKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIVFQPWRLLS 425
           TKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIVFQPWRLLS
Sbjct: 378 TKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIVFQPWRLLS 437

Query: 426 SSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSLSPTGAYYYSGGFNLAA 485
           SSESFVYTWLVGYSALLGPIGGVMV DYY +R+REL VEDLYS SPTGAYYYSGGFNL A
Sbjct: 438 SSESFVYTWLVGYSALLGPIGGVMVADYYFLRKRELIVEDLYSSSPTGAYYYSGGFNLTA 497

Query: 486 VAALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGNAWFISSFLAGFCYWSLSVLFKRRKV 545
           VAALVVGVLPVIPGFLEKV+IVSKVP+VF+VIYGNAWFISSF+AGFCYW LSVL KRRKV
Sbjct: 498 VAALVVGVLPVIPGFLEKVKIVSKVPEVFTVIYGNAWFISSFIAGFCYWGLSVLLKRRKV 557

Query: 546 STSSRFGPLL 556
             SS  GP L
Sbjct: 558 --SSCLGPQL 565

BLAST of Lcy06g020850 vs. ExPASy TrEMBL
Match: A0A6J1GDF7 (purine-uracil permease NCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111453146 PE=3 SV=1)

HSP 1 Score: 957.2 bits (2473), Expect = 3.0e-275
Identity = 487/550 (88.55%), Postives = 511/550 (92.91%), Query Frame = 0

Query: 6   INLHLHRHTFHFPPSLSAVRRRPSVSPLRFSPITPKLLLKIRSNPNPNFTPMASAADRST 65
           +NLHLH H F FP  L+ VRR P+VS L+FSPITPKLLL+ R N N NF PMASAAD  T
Sbjct: 18  VNLHLHHHNFRFPACLTTVRRHPTVSLLQFSPITPKLLLRHRFNSNTNFIPMASAADNPT 77

Query: 66  EDPHLVDSGSEDLRPTTLEQRTLSWPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQGIA 125
           ++ H+ +SGS+DLRPT  EQRTL+WPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQGIA
Sbjct: 78  DNTHVENSGSDDLRPTMPEQRTLAWPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQGIA 137

Query: 126 TVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETW 185
           TVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETW
Sbjct: 138 TVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETW 197

Query: 186 IAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFACFLAFWVVQLASVWKGMDGIRKLETF 245
           IAGEAIFLLLPESIK SSFSQVLPWLGTSPLAF CFLAFWVVQLASVWKGMDGIRKLET 
Sbjct: 198 IAGEAIFLLLPESIKNSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGIRKLETI 257

Query: 246 SAPILVFLTIALLIWSCVRAGGFGHMLSLTSKLSSSEFWALFSPSLTANISFWATLALNI 305
           SAPILVFLT+ LLIWSCVRAGGFGHMLSL SKLSSSEFWALF PSLTANISFWATLALNI
Sbjct: 258 SAPILVFLTMGLLIWSCVRAGGFGHMLSLNSKLSSSEFWALFFPSLTANISFWATLALNI 317

Query: 306 PDFTRYARSQKDQIIGQLGLPIFMGLFTFVGVAVTSSTGVIFGHVISNPIDLLGHIGGFA 365
           PDFTRYA+SQKDQIIGQLGLP+FMG FTFVGVAVTSSTGVIFGH+ISNPIDLLGHIGGFA
Sbjct: 318 PDFTRYAKSQKDQIIGQLGLPVFMGAFTFVGVAVTSSTGVIFGHIISNPIDLLGHIGGFA 377

Query: 366 TKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIVFQPWRLLS 425
           TKVLAIFGIILATVTTNVAANVVAPANALVNL+PSYFTFTRGALLTAVIGIVFQPWRLLS
Sbjct: 378 TKVLAIFGIILATVTTNVAANVVAPANALVNLNPSYFTFTRGALLTAVIGIVFQPWRLLS 437

Query: 426 SSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSLSPTGAYYYSGGFNLAA 485
           SSESFVYTWLVGYSALLGPIGGVMV DYY IRRREL VEDLYS SPTGAYY+SGGFNL A
Sbjct: 438 SSESFVYTWLVGYSALLGPIGGVMVADYYFIRRRELIVEDLYSSSPTGAYYFSGGFNLTA 497

Query: 486 VAALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGNAWFISSFLAGFCYWSLSVLFKRRKV 545
           VAALVVGVLPVIPGFLEKV++VSKVP++F+VIYGNAWFIS+F+AGFCY  LSVL KRRKV
Sbjct: 498 VAALVVGVLPVIPGFLEKVKLVSKVPQLFTVIYGNAWFISTFIAGFCYLGLSVLLKRRKV 557

Query: 546 STSSRFGPLL 556
             SS  GP L
Sbjct: 558 --SSWLGPQL 565

BLAST of Lcy06g020850 vs. ExPASy TrEMBL
Match: A0A0A0LAP3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G746620 PE=3 SV=1)

HSP 1 Score: 956.1 bits (2470), Expect = 6.6e-275
Identity = 492/558 (88.17%), Postives = 510/558 (91.40%), Query Frame = 0

Query: 1   MVTQCINLHLHRHTFHFPPSLSAVRRRP--SVSPLRFSPITPKLLLKIRSNPNPNFT-PM 60
           MVT CINLHLHRH FH PPS S +R RP  S SPL FSPITPKLLLK RS  N  FT PM
Sbjct: 1   MVTHCINLHLHRHIFHLPPSFSTLRYRPTASPSPLPFSPITPKLLLKRRS--NLQFTSPM 60

Query: 61  ASAADRSTEDPHLVDSGSEDLRPTTLEQRTLSWPEMALLWIGLVVGVPAYYLAGSLVDLG 120
           ASA     ED    DSG EDLRPTTLEQRTLSWPEMALLWIGL+VGVP+YYLAGSLVDLG
Sbjct: 61  ASATANPPEDTQNEDSGKEDLRPTTLEQRTLSWPEMALLWIGLIVGVPSYYLAGSLVDLG 120

Query: 121 MAWWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGC 180
           M+WWQGIATVVAANAVTLIALIL GHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGC
Sbjct: 121 MSWWQGIATVVAANAVTLIALILNGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGC 180

Query: 181 GWYGIETWIAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFACFLAFWVVQLASVWKGMD 240
           GWYGIETWIAGEAI LLLP+SIKKS+FSQ LPWLGTSPLAF CFL FWV+QLASVWKGMD
Sbjct: 181 GWYGIETWIAGEAISLLLPQSIKKSAFSQTLPWLGTSPLAFTCFLGFWVIQLASVWKGMD 240

Query: 241 GIRKLETFSAPILVFLTIALLIWSCVRAGGFGHMLSLTSKLSSSEFWALFSPSLTANISF 300
           GIRKLETFSAPILVFLT+ LLIWSCV+AGGFGHMLSLTSKLS SEFWALF PSLTANISF
Sbjct: 241 GIRKLETFSAPILVFLTVCLLIWSCVKAGGFGHMLSLTSKLSFSEFWALFFPSLTANISF 300

Query: 301 WATLALNIPDFTRYARSQKDQIIGQLGLPIFMGLFTFVGVAVTSSTGVIFGHVISNPIDL 360
           WATLALNIPDFTRYA+SQKDQIIGQLGLPIFMG FTFVGVAVTSSTGVIFGH+ISNPIDL
Sbjct: 301 WATLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIDL 360

Query: 361 LGHIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIV 420
           L HIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGI+
Sbjct: 361 LDHIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIL 420

Query: 421 FQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSLSPTGAYYY 480
           FQPWRLLSSSESFVYTWLVGYSALLGPIGGVM+TDYYLIR +ELSVEDLYS SPT AYYY
Sbjct: 421 FQPWRLLSSSESFVYTWLVGYSALLGPIGGVMMTDYYLIRGKELSVEDLYSTSPTAAYYY 480

Query: 481 SGGFNLAAVAALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGNAWFISSFLAGFCYWSLS 540
           SGGFNL AV ALVVGVLPVIPGFLEKVE+VSKVPKVFSVIYG+AWF+S+FLAGFCYW LS
Sbjct: 481 SGGFNLVAVVALVVGVLPVIPGFLEKVELVSKVPKVFSVIYGSAWFVSTFLAGFCYWGLS 540

Query: 541 VLFKRRKVSTSSRFGPLL 556
           VL KRRK S   RFGP L
Sbjct: 541 VLLKRRKASL--RFGPPL 554

BLAST of Lcy06g020850 vs. NCBI nr
Match: XP_038906223.1 (purine-uracil permease NCS1 [Benincasa hispida])

HSP 1 Score: 977.6 bits (2526), Expect = 4.4e-281
Identity = 504/562 (89.68%), Postives = 524/562 (93.24%), Query Frame = 0

Query: 1   MVTQCINLHLHRHTFHFPPSLSAVRRR----PSV--SPLRFSPITPKLLLKIRSNPNPNF 60
           MVTQCINLHLH H FH PPSLSA+R R    P+V  SPL FSP+TPKLLLK RS  N  F
Sbjct: 1   MVTQCINLHLHHHIFHLPPSLSALRHRHRHHPTVSQSPLLFSPVTPKLLLKRRS--NLKF 60

Query: 61  T-PMASAADRSTEDPHLVDSGSEDLRPTTLEQRTLSWPEMALLWIGLVVGVPAYYLAGSL 120
           T PMASA D  +ED    DSG EDLRPTT EQRTLSWPEMALLW+GL+VGVPAYYLAGSL
Sbjct: 61  TPPMASAVDHPSEDTQNEDSGREDLRPTTPEQRTLSWPEMALLWVGLIVGVPAYYLAGSL 120

Query: 121 VDLGMAWWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRA 180
           VDLGM+WWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRA
Sbjct: 121 VDLGMSWWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRA 180

Query: 181 LIGCGWYGIETWIAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFACFLAFWVVQLASVW 240
           LIGCGWYGIETWIAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAF CFLAFW+VQLASVW
Sbjct: 181 LIGCGWYGIETWIAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFICFLAFWIVQLASVW 240

Query: 241 KGMDGIRKLETFSAPILVFLTIALLIWSCVRAGGFGHMLSLTSKLSSSEFWALFSPSLTA 300
           KGMDGIRKLE FSAPILVFLT+ LLIWSCVRAGG GHMLSLTSKLSSSEFWALF PSLTA
Sbjct: 241 KGMDGIRKLEIFSAPILVFLTVCLLIWSCVRAGGLGHMLSLTSKLSSSEFWALFFPSLTA 300

Query: 301 NISFWATLALNIPDFTRYARSQKDQIIGQLGLPIFMGLFTFVGVAVTSSTGVIFGHVISN 360
           NISFWATLALNIPDFTRYA+SQKDQIIGQLGLPIFMG FTFVGVAVTSSTG+IFGH+ISN
Sbjct: 301 NISFWATLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGLIFGHIISN 360

Query: 361 PIDLLGHIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAV 420
           PI+LLGHIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNL+PSYFTFTRGALLTAV
Sbjct: 361 PIELLGHIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLNPSYFTFTRGALLTAV 420

Query: 421 IGIVFQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSLSPTG 480
           IGI+FQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYS SPTG
Sbjct: 421 IGILFQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSTSPTG 480

Query: 481 AYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGNAWFISSFLAGFCY 540
           AYYY+GGFNLAAVAALVVGVLPVIPGFLEKV+IVSKVPKVFSVIYGNAWFIS+FLAGFCY
Sbjct: 481 AYYYTGGFNLAAVAALVVGVLPVIPGFLEKVKIVSKVPKVFSVIYGNAWFISTFLAGFCY 540

Query: 541 WSLSVLFKRRKVSTSSRFGPLL 556
           W LS+L KRRK+  S RFGPLL
Sbjct: 541 WGLSILLKRRKL--SRRFGPLL 558

BLAST of Lcy06g020850 vs. NCBI nr
Match: XP_008442760.1 (PREDICTED: purine-uracil permease NCS1 [Cucumis melo] >KAA0043951.1 purine-uracil permease NCS1 [Cucumis melo var. makuwa] >TYK25190.1 purine-uracil permease NCS1 [Cucumis melo var. makuwa])

HSP 1 Score: 973.0 bits (2514), Expect = 1.1e-279
Identity = 501/558 (89.78%), Postives = 517/558 (92.65%), Query Frame = 0

Query: 1   MVTQCINLHLHRHTFHFPPSLSAVRRRPSV--SPLRFSPITPKLLLKIRSNPNPNFT-PM 60
           MVT CINLHLHRH FH PPS+S +R RP+V  SPL FSPITPKLLLK RS  N  FT PM
Sbjct: 1   MVTHCINLHLHRHIFHLPPSISTLRYRPTVSHSPLLFSPITPKLLLKRRS--NLRFTSPM 60

Query: 61  ASAADRSTEDPHLVDSGSEDLRPTTLEQRTLSWPEMALLWIGLVVGVPAYYLAGSLVDLG 120
           ASA    +ED    DSG +DLRPTTLEQRTLSWPEMALLWIGL+VGVP+YYLAGSLVDLG
Sbjct: 61  ASADANPSEDTQNDDSGKDDLRPTTLEQRTLSWPEMALLWIGLIVGVPSYYLAGSLVDLG 120

Query: 121 MAWWQGIATVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGC 180
           M+WWQGIATVV ANAVTLIALIL GHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGC
Sbjct: 121 MSWWQGIATVVVANAVTLIALILNGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGC 180

Query: 181 GWYGIETWIAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFACFLAFWVVQLASVWKGMD 240
           GWYGIETWIAGEAIFLLLPESIKKSSFSQ+LPWLGTSPLAF CFLAFWVVQLASVWKGMD
Sbjct: 181 GWYGIETWIAGEAIFLLLPESIKKSSFSQILPWLGTSPLAFTCFLAFWVVQLASVWKGMD 240

Query: 241 GIRKLETFSAPILVFLTIALLIWSCVRAGGFGHMLSLTSKLSSSEFWALFSPSLTANISF 300
           GIRKLETFSAPILVFLT+ LLIWSCV+AGGFGHMLSLTSKLSSSEFWALF PSLTANISF
Sbjct: 241 GIRKLETFSAPILVFLTVCLLIWSCVKAGGFGHMLSLTSKLSSSEFWALFFPSLTANISF 300

Query: 301 WATLALNIPDFTRYARSQKDQIIGQLGLPIFMGLFTFVGVAVTSSTGVIFGHVISNPIDL 360
           WATLALNIPDFTRYA+SQKDQIIGQLGLPIFMG FTFVGVAVTSSTGVIFGH+ISNPIDL
Sbjct: 301 WATLALNIPDFTRYAKSQKDQIIGQLGLPIFMGAFTFVGVAVTSSTGVIFGHIISNPIDL 360

Query: 361 LGHIGGFATKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIV 420
           LGHIGGF TKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGI+
Sbjct: 361 LGHIGGFVTKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIL 420

Query: 421 FQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSLSPTGAYYY 480
           FQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIR +ELSVEDLYS SP GAYYY
Sbjct: 421 FQPWRLLSSSESFVYTWLVGYSALLGPIGGVMVTDYYLIRGKELSVEDLYSTSPRGAYYY 480

Query: 481 SGGFNLAAVAALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGNAWFISSFLAGFCYWSLS 540
           SGGFNL AV ALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYG+AWFISSFLAGFCYW LS
Sbjct: 481 SGGFNLVAVVALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGSAWFISSFLAGFCYWGLS 540

Query: 541 VLFKRRKVSTSSRFGPLL 556
           VL KRRK S   RFGPLL
Sbjct: 541 VLLKRRKASL--RFGPLL 554

BLAST of Lcy06g020850 vs. NCBI nr
Match: XP_023005190.1 (purine-uracil permease NCS1-like [Cucurbita maxima])

HSP 1 Score: 968.8 bits (2503), Expect = 2.0e-278
Identity = 492/550 (89.45%), Postives = 513/550 (93.27%), Query Frame = 0

Query: 6   INLHLHRHTFHFPPSLSAVRRRPSVSPLRFSPITPKLLLKIRSNPNPNFTPMASAADRST 65
           +NLHLHRH F FPP L+ VRR P+VS LRFSPITPKLLL+ R   N NF PMASAA+  T
Sbjct: 18  VNLHLHRHNFRFPPCLTTVRRHPTVSLLRFSPITPKLLLRHRFKSNTNFIPMASAANNPT 77

Query: 66  EDPHLVDSGSEDLRPTTLEQRTLSWPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQGIA 125
           ++ H+ + GS+DLRPTT EQRTL+WPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQGIA
Sbjct: 78  DNTHVENPGSDDLRPTTPEQRTLAWPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQGIA 137

Query: 126 TVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETW 185
           TVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETW
Sbjct: 138 TVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETW 197

Query: 186 IAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFACFLAFWVVQLASVWKGMDGIRKLETF 245
           IAGEAIFLLLPESIK SSFSQVLPWLGTSPLAF CFLAFWVVQLASVWKGMDGIRKLET 
Sbjct: 198 IAGEAIFLLLPESIKNSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGIRKLETI 257

Query: 246 SAPILVFLTIALLIWSCVRAGGFGHMLSLTSKLSSSEFWALFSPSLTANISFWATLALNI 305
           SAPILVFLT+ LLIWSCVRAGGFGHMLSL SKLSSSEFWALF PSLTANISFWATLALNI
Sbjct: 258 SAPILVFLTMGLLIWSCVRAGGFGHMLSLNSKLSSSEFWALFFPSLTANISFWATLALNI 317

Query: 306 PDFTRYARSQKDQIIGQLGLPIFMGLFTFVGVAVTSSTGVIFGHVISNPIDLLGHIGGFA 365
           PDFTRYA+SQKDQIIGQLGLP+FMG FTFVGVAVTSSTGVIFGH+ISNPIDLLGHIGGFA
Sbjct: 318 PDFTRYAKSQKDQIIGQLGLPVFMGAFTFVGVAVTSSTGVIFGHIISNPIDLLGHIGGFA 377

Query: 366 TKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIVFQPWRLLS 425
           TKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIVFQPWRLLS
Sbjct: 378 TKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIVFQPWRLLS 437

Query: 426 SSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSLSPTGAYYYSGGFNLAA 485
           SSESFVYTWLVGYSALLGPIGGVMV DYY +R+REL VEDLYS SPTGAYYYSGGFNL A
Sbjct: 438 SSESFVYTWLVGYSALLGPIGGVMVADYYFLRKRELIVEDLYSSSPTGAYYYSGGFNLTA 497

Query: 486 VAALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGNAWFISSFLAGFCYWSLSVLFKRRKV 545
           VAALVVGVLPVIPGFLEKV+IVSKVP+VF+VIYGNAWFISSF+AGFCYW LSVL KRRKV
Sbjct: 498 VAALVVGVLPVIPGFLEKVKIVSKVPEVFTVIYGNAWFISSFIAGFCYWGLSVLLKRRKV 557

Query: 546 STSSRFGPLL 556
             SS  GP L
Sbjct: 558 --SSCLGPQL 565

BLAST of Lcy06g020850 vs. NCBI nr
Match: XP_023539092.1 (purine-uracil permease NCS1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 966.8 bits (2498), Expect = 7.7e-278
Identity = 491/550 (89.27%), Postives = 512/550 (93.09%), Query Frame = 0

Query: 6   INLHLHRHTFHFPPSLSAVRRRPSVSPLRFSPITPKLLLKIRSNPNPNFTPMASAADRST 65
           ++LHLH H F  PP+LS VRR P+VS L FSPITPKLLL+ R   N NFT MASAAD  T
Sbjct: 18  VSLHLHHHNFRLPPALSTVRRHPTVSLLHFSPITPKLLLRHRFKSNTNFTAMASAADNPT 77

Query: 66  EDPHLVDSGSEDLRPTTLEQRTLSWPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQGIA 125
           ++ H+ +SGS+DLRPTT EQRTL+WPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQGIA
Sbjct: 78  DNTHVENSGSDDLRPTTPEQRTLAWPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQGIA 137

Query: 126 TVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETW 185
           TVVAANAVTLIALILTGHPGTKYGISFP+FARSSFGIHGAHIPTLLRALIGCGWYGIETW
Sbjct: 138 TVVAANAVTLIALILTGHPGTKYGISFPIFARSSFGIHGAHIPTLLRALIGCGWYGIETW 197

Query: 186 IAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFACFLAFWVVQLASVWKGMDGIRKLETF 245
           IAGEAIFLLLPESIK SSFSQVLPWLGTSPLAF CFLAFWVVQLASVWKGMDGIRKLET 
Sbjct: 198 IAGEAIFLLLPESIKNSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGIRKLETI 257

Query: 246 SAPILVFLTIALLIWSCVRAGGFGHMLSLTSKLSSSEFWALFSPSLTANISFWATLALNI 305
           SAPILVFLT+ LLIWSCVRAGGFGHMLSL SKLSSSEFWALF PSLTANISFWATLALNI
Sbjct: 258 SAPILVFLTMGLLIWSCVRAGGFGHMLSLNSKLSSSEFWALFFPSLTANISFWATLALNI 317

Query: 306 PDFTRYARSQKDQIIGQLGLPIFMGLFTFVGVAVTSSTGVIFGHVISNPIDLLGHIGGFA 365
           PDFTRYA+SQKDQIIGQLGLP+FMG FTFVGVAVTSSTGVIFGH+ISNPIDLLGHIGGFA
Sbjct: 318 PDFTRYAKSQKDQIIGQLGLPVFMGAFTFVGVAVTSSTGVIFGHIISNPIDLLGHIGGFA 377

Query: 366 TKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIVFQPWRLLS 425
           TKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIVFQPWRLLS
Sbjct: 378 TKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIVFQPWRLLS 437

Query: 426 SSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSLSPTGAYYYSGGFNLAA 485
           SSESFVYTWLVGYSALLGPIGGVMV DYY IRRREL VEDLYSLSPTGAYYYSGGFNL A
Sbjct: 438 SSESFVYTWLVGYSALLGPIGGVMVADYYFIRRRELIVEDLYSLSPTGAYYYSGGFNLTA 497

Query: 486 VAALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGNAWFISSFLAGFCYWSLSVLFKRRKV 545
           V ALVVGVLPVIPGFLEKV+IVSKVP+VF+VIYGNAWFIS+F+AGFCYW LSVL KRRKV
Sbjct: 498 VTALVVGVLPVIPGFLEKVKIVSKVPQVFTVIYGNAWFISTFIAGFCYWGLSVLLKRRKV 557

Query: 546 STSSRFGPLL 556
             SS  GP L
Sbjct: 558 --SSCLGPQL 565

BLAST of Lcy06g020850 vs. NCBI nr
Match: KAG7028053.1 (Purine-uracil permease NCS1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 963.4 bits (2489), Expect = 8.6e-277
Identity = 489/550 (88.91%), Postives = 511/550 (92.91%), Query Frame = 0

Query: 6   INLHLHRHTFHFPPSLSAVRRRPSVSPLRFSPITPKLLLKIRSNPNPNFTPMASAADRST 65
           +N+HLH H F FP  L+ VRR P+VS L+FSPITPKLLL+     N NF PMASAAD  T
Sbjct: 7   VNVHLHHHNFRFPACLTTVRRHPTVSLLQFSPITPKLLLRHGFKSNTNFIPMASAADNPT 66

Query: 66  EDPHLVDSGSEDLRPTTLEQRTLSWPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQGIA 125
           ++ H+ +SGS+DLRPTTLEQRTL+WPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQGIA
Sbjct: 67  DNTHVENSGSDDLRPTTLEQRTLAWPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQGIA 126

Query: 126 TVVAANAVTLIALILTGHPGTKYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETW 185
           TVVAANAVTLIALILTGHPGTKYGISFPVF RSSFGIHGAHIPTLLRALIGCGWYGIETW
Sbjct: 127 TVVAANAVTLIALILTGHPGTKYGISFPVFVRSSFGIHGAHIPTLLRALIGCGWYGIETW 186

Query: 186 IAGEAIFLLLPESIKKSSFSQVLPWLGTSPLAFACFLAFWVVQLASVWKGMDGIRKLETF 245
           IAGEAIFLLLPESIK SSFSQVLPWLGTSPLAF CFLAFWVVQLASVWKGMDGIRKLET 
Sbjct: 187 IAGEAIFLLLPESIKNSSFSQVLPWLGTSPLAFTCFLAFWVVQLASVWKGMDGIRKLETI 246

Query: 246 SAPILVFLTIALLIWSCVRAGGFGHMLSLTSKLSSSEFWALFSPSLTANISFWATLALNI 305
           SAPILVFLT+ LLIWSCVRAGGFGHMLSL SKLSSSEFWALF PSLTANISFWATLALNI
Sbjct: 247 SAPILVFLTMGLLIWSCVRAGGFGHMLSLNSKLSSSEFWALFFPSLTANISFWATLALNI 306

Query: 306 PDFTRYARSQKDQIIGQLGLPIFMGLFTFVGVAVTSSTGVIFGHVISNPIDLLGHIGGFA 365
           PDFTRYA+SQKDQIIGQLGLP+FMG FTFVGVAVTSSTGVIFGH+ISNPIDLLGHIGGFA
Sbjct: 307 PDFTRYAKSQKDQIIGQLGLPVFMGAFTFVGVAVTSSTGVIFGHIISNPIDLLGHIGGFA 366

Query: 366 TKVLAIFGIILATVTTNVAANVVAPANALVNLSPSYFTFTRGALLTAVIGIVFQPWRLLS 425
           TKVLAIFGIILATVTTNVAANVVAPANALVNL+PSYFTFTRGALLTAVIGIVFQPWRLLS
Sbjct: 367 TKVLAIFGIILATVTTNVAANVVAPANALVNLNPSYFTFTRGALLTAVIGIVFQPWRLLS 426

Query: 426 SSESFVYTWLVGYSALLGPIGGVMVTDYYLIRRRELSVEDLYSLSPTGAYYYSGGFNLAA 485
           SSESFVYTWLVGYSALLGPIGGVMV DYY IRRREL VEDLYS SPTGAYYYSGGFNL A
Sbjct: 427 SSESFVYTWLVGYSALLGPIGGVMVADYYFIRRRELIVEDLYSSSPTGAYYYSGGFNLTA 486

Query: 486 VAALVVGVLPVIPGFLEKVEIVSKVPKVFSVIYGNAWFISSFLAGFCYWSLSVLFKRRKV 545
           VAALVVGVLPVIPGFLEKV+IVSKVP+VF+VIYGNAWFIS+F+AGFCYW LSVL KRRKV
Sbjct: 487 VAALVVGVLPVIPGFLEKVKIVSKVPQVFTVIYGNAWFISTFIAGFCYWGLSVLLKRRKV 546

Query: 546 STSSRFGPLL 556
             SS  GP L
Sbjct: 547 --SSLLGPQL 554

BLAST of Lcy06g020850 vs. TAIR 10
Match: AT5G03555.1 (permease, cytosine/purines, uracil, thiamine, allantoin family protein )

HSP 1 Score: 711.1 bits (1834), Expect = 7.1e-205
Identity = 359/529 (67.86%), Postives = 421/529 (79.58%), Query Frame = 0

Query: 35  FSPITP--KLLLKIRSNPNPNFTPMASAA------DRSTEDPHLVDSGSEDLRPTTLEQR 94
           FSPI P   L+L       P  + M  +       D S  DP L    ++DL+PTT  QR
Sbjct: 74  FSPILPGDNLVLSRSGVIRPRLSAMTGSEINDHGYDESQFDPSLT---NDDLKPTTPSQR 133

Query: 95  TLSWPEMALLWIGLVVGVPAYYLAGSLVDLGMAWWQGIATVVAANAVTLIALILTGHPGT 154
           T SW +M+ LWIGLVVGVP YYLAGSLVDLGMAWWQGIATVV AN + L+ L+LT  PGT
Sbjct: 134 TFSWLDMSSLWIGLVVGVPTYYLAGSLVDLGMAWWQGIATVVTANLILLVPLVLTAQPGT 193

Query: 155 KYGISFPVFARSSFGIHGAHIPTLLRALIGCGWYGIETWIAGEAIFLLLPESIKKSSFSQ 214
            YGISFPV ARSSFGI GAHIPTLLRAL+GCGWYGIETWI GEAIFLLLP  IKKS+ S 
Sbjct: 194 LYGISFPVLARSSFGIRGAHIPTLLRALVGCGWYGIETWIGGEAIFLLLPGHIKKSALSH 253

Query: 215 VLPWLGTSPLAFACFLAFWVVQLASVWKGMDGIRKLETFSAPILVFLTIALLIWSCVRAG 274
            LPWLGTSPL F+CF+ FW+ QL  VW+GMDGIRKLE +SAPIL+ LT  LL WS ++AG
Sbjct: 254 TLPWLGTSPLEFSCFIVFWLAQLCIVWRGMDGIRKLEKYSAPILISLTSCLLAWSYLKAG 313

Query: 275 GFGHMLSLTSKLSSSEFWALFSPSLTANISFWATLALNIPDFTRYARSQKDQIIGQLGLP 334
           GFGHMLSL+SKL+S++FW LF PSLTANISFWATLALNIPDF+R+A+SQ DQIIGQ+GLP
Sbjct: 314 GFGHMLSLSSKLTSAQFWTLFFPSLTANISFWATLALNIPDFSRFAKSQTDQIIGQVGLP 373

Query: 335 IFMGLFTFVGVAVTSSTGVIFGHVISNPIDLLGHIGGFATKVLAIFGIILATVTTNVAAN 394
           +FMGLFTFVGVAVTSST +IFG VISNPI+LLG IGG AT +LAI GI LAT+TTN+AAN
Sbjct: 374 VFMGLFTFVGVAVTSSTSIIFGRVISNPIELLGQIGGLATTLLAIVGISLATLTTNIAAN 433

Query: 395 VVAPANALVNLSPSYFTFTRGALLTAVIGIVFQPWRLLSSSESFVYTWLVGYSALLGPIG 454
           VVAPANALVNL+P +FTF RGA LTAV+GIVFQPWRLL SSESFVYTWL+GYSALLGPIG
Sbjct: 434 VVAPANALVNLNPKFFTFGRGAFLTAVLGIVFQPWRLLKSSESFVYTWLIGYSALLGPIG 493

Query: 455 GVMVTDYYLIRRRELSVEDLYSLSPTGAYYYSGGFNLAAVAALVVGVLPVIPGFLEKVEI 514
           G+++ DYYLI++ +L++ DLYSLSP+G YY+S G+N+AAV ALV G++PV+PGFL K+  
Sbjct: 494 GIILVDYYLIKKMKLNIGDLYSLSPSGEYYFSKGYNVAAVVALVAGIIPVVPGFLHKISA 553

Query: 515 VSKVPKVFSVIYGNAWFISSFLAGFCYWSLSVLFKRRKVSTSSRFGPLL 556
           +SK+   F V+Y NA F S  +AGF YW +     R++ S SS   PLL
Sbjct: 554 LSKISNGFVVVYDNALFFSFIIAGFVYWIIMSRLGRKQSSLSSSSHPLL 599

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LZD01.0e-20367.86Purine-uracil permease NCS1 OS=Arabidopsis thaliana OX=3702 GN=NCS1 PE=1 SV=1[more]
P945751.7e-7837.37Probable allantoin permease OS=Bacillus subtilis (strain 168) OX=224308 GN=pucI ... [more]
O140358.6e-6234.26Uncharacterized permease C29B12.14c OS=Schizosaccharomyces pombe (strain 972 / A... [more]
Q102792.4e-5632.12Uracil permease OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812... [more]
Q048951.9e-4828.63Allantoin permease OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=5... [more]
Match NameE-valueIdentityDescription
A0A5D3DPI85.2e-28089.78Purine-uracil permease NCS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3B7565.2e-28089.78purine-uracil permease NCS1 OS=Cucumis melo OX=3656 GN=LOC103486539 PE=3 SV=1[more]
A0A6J1KWR99.9e-27989.45purine-uracil permease NCS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498292 PE=... [more]
A0A6J1GDF73.0e-27588.55purine-uracil permease NCS1-like OS=Cucurbita moschata OX=3662 GN=LOC111453146 P... [more]
A0A0A0LAP36.6e-27588.17Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G746620 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038906223.14.4e-28189.68purine-uracil permease NCS1 [Benincasa hispida][more]
XP_008442760.11.1e-27989.78PREDICTED: purine-uracil permease NCS1 [Cucumis melo] >KAA0043951.1 purine-uraci... [more]
XP_023005190.12.0e-27889.45purine-uracil permease NCS1-like [Cucurbita maxima][more]
XP_023539092.17.7e-27889.27purine-uracil permease NCS1 [Cucurbita pepo subsp. pepo][more]
KAG7028053.18.6e-27788.91Purine-uracil permease NCS1, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
Match NameE-valueIdentityDescription
AT5G03555.17.1e-20567.86permease, cytosine/purines, uracil, thiamine, allantoin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.4160.10Hydantoin permeasecoord: 72..531
e-value: 7.3E-117
score: 392.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 49..76
NoneNo IPR availablePANTHERPTHR30618:SF0PURINE-URACIL PERMEASE NCS1coord: 60..541
NoneNo IPR availablePANTHERPTHR30618NCS1 FAMILY PURINE/PYRIMIDINE TRANSPORTERcoord: 60..541
NoneNo IPR availableCDDcd11485SLC-NCS1sbd_YbbW-likecoord: 75..537
e-value: 0.0
score: 524.055
IPR012681Nucleobase cation symporter-1, NCS1TIGRFAMTIGR00800TIGR00800coord: 75..506
e-value: 5.9E-107
score: 356.1
IPR001248Purine-cytosine permeasePFAMPF02133Transp_cyt_purcoord: 82..524
e-value: 2.6E-103
score: 346.2

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy06g020850.1Lcy06g020850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015851 nucleobase transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015205 nucleobase transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity