Lcy05g021100 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy05g021100
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	transcription elongation factor TFIIS-like
Location	Chr05: 50178812 .. 50180316 (-)
RNA-Seq Expression	Lcy05g021100
Synteny	Lcy05g021100

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAATGAGCTGGTGGAATTGTTTGAAGCAGCGAAGAAGGCTGCAGACGCCGCTGCAGCATCATCAAATGATGGGGGAGTCGAGGAAAGTCGTTGTCTAGATGCCCTACGACAGCTCAAGAAATTTCCTGTTTCTTATCAAATCCTGGTTTCCACTCAGGTTTTCTTCTCATTAGACTTGAAATTTGTTTGTTTTGTTGATGGTGGATTAGTCAAAATGTAAAAAGCCGACTAGTTTGTAAAATCATACATGGTTTTTATTATGAATTAAGCCAAAATTCATTGTTCTTTTTTTTTTTTTGGCTTACAAATTGGTTTCATATCAAGCTGAGTTCACTCTATTTCATCTGTTATTTGAGTTGTTCACATGACGAAATCATCTCACCTGTGACTCTTTAAAATTTTGGAATATAACTCTGGTTGGCGCCGCAATTGTTATATATTCTGTTCTAGAATTCTTATAATTCCTTTTCCCGTGTAATTGAGGTTCATTTGCCTGTGTTGATTGTTCCGTTATCTTGTCCTCTGTAGGTTGGGAAGCGGCTTCGGCACCTCACTAAACACCCCAGGAAGAAAATACAGGACTATGCTTCTGACCTGATTGAGATGTGGAAGGACATAGTAATCAAGGAGACCAACAAGAATAAGAAAAATGGAAATGGAAATGCCAACTGTAAAGAGCCTCCTAAAATTGGTTCTCCCAGTGCAGAGTCCGTTAAGGTTGAGAAGTTTCAAAAATCATCTTCCATGAAGGTTGAGAAAGTTTCCAAGGTTGAGCACTTCGATAGGAATGGTGCTACAAGCTCTGTAAAATTTTCCAGATCAGAAAGTGCCATCTCGGAGAAAAACTCTGTCAAGGTTGAGAAAACTGATTCAGTTGTCAAGGTTGAAAGAATAGTTAAAGAAGAGAAGAAAATCTCAATAGAAAAGAAACCATCAGGTGGGGCTGCTGCACCTCCAAAGCTTACTTCCATGATTAAATCAAAAGATGCTGCTCGAGACAAAATTAGGGAACTTCTCTTTGAGGCCTTCTCCAAGGTTCCTGGCGAGGCTGGCGAAGATGTTATAGATGAAGTAAATGCAAGTGATCCTATCCGTGTTGCTGTATCTGTAGAATCCGTGATGTTTGAAAATTGGGGAGGTTCTACCGGAGCACAAAAGGCCAAGTACAGATCTGTAATGTTTAACCTCAAGGATCCGAAGAACCCAGACTTTCGGAGAAAAGTTCTTCTTGGACTTATCAAGCCAGAAAGGTTGATCAACTTGAGCACAACTGATATGGCAAGTGATCAGAGAAAACGTGAGAATGAGGAGATTGCACAAAAGGCTTTATTTGATTGTGAGCGTGGAGGAGCTCCAAAAGCTACTACTGATCAGTTCAAGTGTGGTCGATGTGGTCAACGCAAGACAACCTACTATCAGTTGCAGACGCGGAGTGCTGATGAACCTATGACCACCTTTGTAACTTGTGTAAACTGCAATAACCATTGGAAGTTCTGTTGA

mRNA sequence

ATGGAGAATGAGCTGGTGGAATTGTTTGAAGCAGCGAAGAAGGCTGCAGACGCCGCTGCAGCATCATCAAATGATGGGGGAGTCGAGGAAAGTCGTTGTCTAGATGCCCTACGACAGCTCAAGAAATTTCCTGTTTCTTATCAAATCCTGGTTTCCACTCAGGTTGGGAAGCGGCTTCGGCACCTCACTAAACACCCCAGGAAGAAAATACAGGACTATGCTTCTGACCTGATTGAGATGTGGAAGGACATAGTAATCAAGGAGACCAACAAGAATAAGAAAAATGGAAATGGAAATGCCAACTGTAAAGAGCCTCCTAAAATTGGTTCTCCCAGTGCAGAGTCCGTTAAGGTTGAGAAGTTTCAAAAATCATCTTCCATGAAGGTTGAGAAAGTTTCCAAGGTTGAGCACTTCGATAGGAATGGTGCTACAAGCTCTGTAAAATTTTCCAGATCAGAAAGTGCCATCTCGGAGAAAAACTCTGTCAAGGTTGAGAAAACTGATTCAGTTGTCAAGGTTGAAAGAATAGTTAAAGAAGAGAAGAAAATCTCAATAGAAAAGAAACCATCAGGTGGGGCTGCTGCACCTCCAAAGCTTACTTCCATGATTAAATCAAAAGATGCTGCTCGAGACAAAATTAGGGAACTTCTCTTTGAGGCCTTCTCCAAGGTTCCTGGCGAGGCTGGCGAAGATGTTATAGATGAAGTAAATGCAAGTGATCCTATCCGTGTTGCTGTATCTGTAGAATCCGTGATGTTTGAAAATTGGGGAGGTTCTACCGGAGCACAAAAGGCCAAGTACAGATCTGTAATGTTTAACCTCAAGGATCCGAAGAACCCAGACTTTCGGAGAAAAGTTCTTCTTGGACTTATCAAGCCAGAAAGGTTGATCAACTTGAGCACAACTGATATGGCAAGTGATCAGAGAAAACGTGAGAATGAGGAGATTGCACAAAAGGCTTTATTTGATTGTGAGCGTGGAGGAGCTCCAAAAGCTACTACTGATCAGTTCAAGTGTGGTCGATGTGGTCAACGCAAGACAACCTACTATCAGTTGCAGACGCGGAGTGCTGATGAACCTATGACCACCTTTGTAACTTGTGTAAACTGCAATAACCATTGGAAGTTCTGTTGA

Coding sequence (CDS)

ATGGAGAATGAGCTGGTGGAATTGTTTGAAGCAGCGAAGAAGGCTGCAGACGCCGCTGCAGCATCATCAAATGATGGGGGAGTCGAGGAAAGTCGTTGTCTAGATGCCCTACGACAGCTCAAGAAATTTCCTGTTTCTTATCAAATCCTGGTTTCCACTCAGGTTGGGAAGCGGCTTCGGCACCTCACTAAACACCCCAGGAAGAAAATACAGGACTATGCTTCTGACCTGATTGAGATGTGGAAGGACATAGTAATCAAGGAGACCAACAAGAATAAGAAAAATGGAAATGGAAATGCCAACTGTAAAGAGCCTCCTAAAATTGGTTCTCCCAGTGCAGAGTCCGTTAAGGTTGAGAAGTTTCAAAAATCATCTTCCATGAAGGTTGAGAAAGTTTCCAAGGTTGAGCACTTCGATAGGAATGGTGCTACAAGCTCTGTAAAATTTTCCAGATCAGAAAGTGCCATCTCGGAGAAAAACTCTGTCAAGGTTGAGAAAACTGATTCAGTTGTCAAGGTTGAAAGAATAGTTAAAGAAGAGAAGAAAATCTCAATAGAAAAGAAACCATCAGGTGGGGCTGCTGCACCTCCAAAGCTTACTTCCATGATTAAATCAAAAGATGCTGCTCGAGACAAAATTAGGGAACTTCTCTTTGAGGCCTTCTCCAAGGTTCCTGGCGAGGCTGGCGAAGATGTTATAGATGAAGTAAATGCAAGTGATCCTATCCGTGTTGCTGTATCTGTAGAATCCGTGATGTTTGAAAATTGGGGAGGTTCTACCGGAGCACAAAAGGCCAAGTACAGATCTGTAATGTTTAACCTCAAGGATCCGAAGAACCCAGACTTTCGGAGAAAAGTTCTTCTTGGACTTATCAAGCCAGAAAGGTTGATCAACTTGAGCACAACTGATATGGCAAGTGATCAGAGAAAACGTGAGAATGAGGAGATTGCACAAAAGGCTTTATTTGATTGTGAGCGTGGAGGAGCTCCAAAAGCTACTACTGATCAGTTCAAGTGTGGTCGATGTGGTCAACGCAAGACAACCTACTATCAGTTGCAGACGCGGAGTGCTGATGAACCTATGACCACCTTTGTAACTTGTGTAAACTGCAATAACCATTGGAAGTTCTGTTGA

Protein sequence

MENELVELFEAAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVSYQILVSTQVGKRLRHLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEKFQKSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEEKKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNASDPIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLSTTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEPMTTFVTCVNCNNHWKFC

Homology

[Close]

BLAST of Lcy05g021100 vs. ExPASy Swiss-Prot
Match: Q9ZVH8 (Transcription elongation factor TFIIS OS=Arabidopsis thaliana OX=3702 GN=TFIIS PE=1 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 5.4e-108
Identity = 221/384 (57.55%), Postives = 269/384 (70.05%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAASS-NDGGVEESRCLDALRQLKKFPVSYQILVSTQVGKRL 60
           ME++L++LFE AKKAADAAA       G E S+C+DAL+QLKKFPV+Y  LV+TQVGK+L
Sbjct: 1   MESDLIDLFEGAKKAADAAALDGVTSSGPEVSQCIDALKQLKKFPVTYDTLVATQVGKKL 60

Query: 61  RHLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAES---- 120
           R L KHP + I+  A+DL+E+WK +VI+ET K KK   G   CKE  K+     E     
Sbjct: 61  RSLAKHPVEDIKSVATDLLEIWKKVVIEETAKAKKT-EGTNGCKE-AKVNKMDVEKPSNP 120

Query: 121 --VKVEKFQKSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKV 180
             VKV+K Q+  S K  KV + E    N   + VK  R    I   N  K++     VK 
Sbjct: 121 APVKVQKLQRGDSAKSIKVERKE--PDNKVVTGVKIERKVPDIKVTNGTKIDYRGQAVKD 180

Query: 181 ERIVKEEKKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVI 240
           E++ K+ +  S  K P+  A APPKLT+M+K  D  RDKIRELL EA  +V GEA +   
Sbjct: 181 EKVSKDNQ--SSMKAPAKAANAPPKLTAMLKCNDPVRDKIRELLVEALCRVAGEADDYER 240

Query: 241 DEVNASDPIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKP 300
           + VNASDP+RVAVSVES+MFE  G STGAQK KYRS+MFNL+D  NPD RR+VL G I P
Sbjct: 241 ESVNASDPLRVAVSVESLMFEKLGRSTGAQKLKYRSIMFNLRDSNNPDLRRRVLTGEISP 300

Query: 301 ERLINLSTTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQ 360
           E+LI LS  DMASD+RK+EN +I +KALFDCERG A KA+TDQFKCGRCGQRK TYYQ+Q
Sbjct: 301 EKLITLSAEDMASDKRKQENNQIKEKALFDCERGLAAKASTDQFKCGRCGQRKCTYYQMQ 360

Query: 361 TRSADEPMTTFVTCVNCNNHWKFC 378
           TRSADEPMTT+VTCVNC+NHWKFC
Sbjct: 361 TRSADEPMTTYVTCVNCDNHWKFC 378

[Close]

BLAST of Lcy05g021100 vs. ExPASy Swiss-Prot
Match: P20232 (Transcription elongation factor S-II OS=Drosophila melanogaster OX=7227 GN=TfIIS PE=2 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 5.0e-29
Identity = 109/374 (29.14%), Postives = 160/374 (42.78%), Query Frame = 0


Query: 7   ELFEAAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVSYQILVSTQVGKRLRHLTKHP 66
           E+F   KK +  A+      G  + + LD L+ L+   ++  IL  T++G  +  L K  
Sbjct: 6   EVFRIQKKMSKMAS-----DGTGQDQALDLLKALQTLNINLDILTKTRIGMTVNELRKSS 65

Query: 67  R-KKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEKFQKSS 126
           +  ++   A  LI+ WK  +             N++ KE     S +++S    K   S 
Sbjct: 66  KDDEVIALAKTLIKNWKRFLASPAPTTP----NNSSAKEGSSNNSSASKSTSAAKSSSSI 125

Query: 127 SMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEEKKISI 186
           S K                      +S S+ S K+  K   T S              S 
Sbjct: 126 SGK---------------------DKSSSSSSSKDKEKKGSTSS--------------SQ 185

Query: 187 EKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEA--FSKVPGEAGEDVIDEVNASDPIR 246
              PSGG              DA R K RE+L  A    +VP   GE          P  
Sbjct: 186 TSFPSGGMT------------DAVRIKCREMLATALKIGEVPEGCGE----------PEE 245

Query: 247 VAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLSTTD 306
           +A  +E  ++  +  +    K + RS + NLKDPKNP  R   + G +  ++L  ++  +
Sbjct: 246 MAAELEDAIYSEFNNTDMKYKNRIRSRVANLKDPKNPGLRGNFMCGAVTAKQLAKMTPEE 305

Query: 307 MASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEPMTT 366
           MASD+ K+  E+  ++A+ D +        TD  KC +C +R  TY QLQTRSADEPMTT
Sbjct: 306 MASDEMKKLREKFVKEAINDAQLATVQGTKTDLLKCAKCKKRNCTYNQLQTRSADEPMTT 313

Query: 367 FVTCVNCNNHWKFC 378
           FV C  C N WKFC
Sbjct: 366 FVMCNECGNRWKFC 313

[Close]

BLAST of Lcy05g021100 vs. ExPASy Swiss-Prot
Match: Q2KI09 (Transcription elongation factor A protein 3 OS=Bos taurus OX=9913 GN=TCEA3 PE=2 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 9.4e-28
Identity = 110/380 (28.95%), Postives = 171/380 (45.00%), Query Frame = 0


Query: 7   ELFEAAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVSYQILVSTQVGKRLRHLTKH- 66
           EL   AKK     A    +G       LD L++L  + +S Q+L +T++G  +  + KH 
Sbjct: 6   ELLRIAKKLEKMVARKKTEG------ALDLLKKLGSWQMSIQLLQTTRIGVAVNGVRKHC 65

Query: 67  PRKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEKFQKSS 126
             K++   A  LI  WK ++        + G      K+  K        +    ++  +
Sbjct: 66  SNKEVVALAKVLIRNWKQLLDSPATPKGEKGEERVKAKKKEK-------GLDCSDWKPET 125

Query: 127 SMKVEKVSKVEH--------FDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIV 186
           S+   +  +VE           ++ ATSS K    E + S K+  +  +T S        
Sbjct: 126 SLSPPRKKRVEEPKDRRDSVDSKSSATSSPKRPSMERSNSSKSKAETPRTPS-------- 185

Query: 187 KEEKKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVN 246
                 S    PS    AP  LT      D+ RDK  E+L  A      +A +D  D   
Sbjct: 186 ---SPSSPTFAPSVCLLAPCYLTG-----DSVRDKCVEMLSAAL-----KADDDYKDYGV 245

Query: 247 ASDPIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLI 306
             D  ++A  +E  +++    +    + + RS + NLKDP+NP  RR VL G I    + 
Sbjct: 246 NCD--KMASEIEDHIYQELKSTDMKYRNRVRSRISNLKDPRNPGLRRNVLSGAISAGLIA 305

Query: 307 NLSTTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSA 366
            ++  +MASD+ +     + Q+A+ + +       TTD F+C +C ++  TY Q+QTRSA
Sbjct: 306 KMTAEEMASDELRELRNAMTQEAIREHQMAKTGGTTTDLFQCSKCKKKNCTYNQVQTRSA 349

Query: 367 DEPMTTFVTCVNCNNHWKFC 378
           DEPMTTFV C  C N WKFC
Sbjct: 366 DEPMTTFVLCNECGNRWKFC 349

[Close]

BLAST of Lcy05g021100 vs. ExPASy Swiss-Prot
Match: P23881 (Transcription elongation factor A protein 3 OS=Mus musculus OX=10090 GN=Tcea3 PE=1 SV=3)

HSP 1 Score: 123.2 bits (308), Expect = 6.1e-27
Identity = 111/382 (29.06%), Postives = 170/382 (44.50%), Query Frame = 0


Query: 7   ELFEAAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVSYQILVSTQVGKRLRHLTKH- 66
           EL   AKK     +    +G       LD L++L    +S Q+L +T++G  +  + KH 
Sbjct: 6   ELLRIAKKLEKMVSRKKTEG------ALDLLKKLNSCQMSIQLLQTTRIGVAVNGVRKHC 65

Query: 67  PRKKIQDYASDLIEMWKDIV---------IKETNKNKK-NGNGNANCKEPPKIGSPSAES 126
             K++   A  LI+ WK ++          +E  K KK  G G ++ K    +  P  + 
Sbjct: 66  SDKEVVSLAKVLIKNWKRLLDSPRTTKGEREEREKAKKEKGLGCSDWKPEAGLSPPRKKG 125

Query: 127 VKVEKFQKSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVER 186
               K ++ S              R+  TSS K    E + S K+ V+   T S      
Sbjct: 126 GGEPKTRRDSV-----------DSRSSTTSSPKRPSLERSNSSKSKVETPTTPSSPSTPT 185

Query: 187 IVKEEKKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDE 246
                        P+    AP  LT      D+ RDK  E+L  A         ED   +
Sbjct: 186 FA-----------PAVCLLAPCYLTG-----DSVRDKCVEMLSAALK------AEDNFKD 245

Query: 247 VNASDPIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPER 306
              +   ++A  +E  +++    +    + + RS + NLKDP+NP  RR VL G I PE 
Sbjct: 246 YGVNCD-KLASEIEDHIYQELKSTDMKYRNRVRSRISNLKDPRNPGLRRNVLSGAISPEL 305

Query: 307 LINLSTTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTR 366
           +  ++  +MASD+ +     + Q+A+ + +       TTD  +C +C ++  TY Q+QTR
Sbjct: 306 IAKMTAEEMASDELRELRNAMTQEAIREHQMAKTGGTTTDLLRCSKCKKKNCTYNQVQTR 347

Query: 367 SADEPMTTFVTCVNCNNHWKFC 378
           SADEPMTTFV C  C N WKFC
Sbjct: 366 SADEPMTTFVLCNECGNRWKFC 347

[Close]

BLAST of Lcy05g021100 vs. ExPASy Swiss-Prot
Match: O75764 (Transcription elongation factor A protein 3 OS=Homo sapiens OX=9606 GN=TCEA3 PE=1 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 6.7e-26
Identity = 112/379 (29.55%), Postives = 172/379 (45.38%), Query Frame = 0


Query: 7   ELFEAAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVSYQILVSTQVGKRLRHLTKH- 66
           EL   AKK     A  + +G       LD L++L    +S Q+L +T++G  +  + KH 
Sbjct: 6   ELLRIAKKLEKMVARKNTEG------ALDLLKKLHSCQMSIQLLQTTRIGVAVNGVRKHC 65

Query: 67  PRKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPK--IGSPSAESVKVEKFQK 126
             K++   A  LI+ WK ++               +   PPK   G    ++ K EK  +
Sbjct: 66  SDKEVVSLAKVLIKNWKRLL---------------DSPGPPKGEKGEEREKAKKKEKGLE 125

Query: 127 SSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEEKKI 186
            S  K E         R         +R +S  S+ ++    K  S   VER    + K 
Sbjct: 126 CSDWKPEAGLSPPRKKREDPK-----TRRDSVDSKSSASSSPKRPS---VERSNSSKSKA 185

Query: 187 SIEKKPSGG-----AAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNA 246
              K PS       A++   L     + D+ RDK  E+L  A      +A +D  D    
Sbjct: 186 ESPKTPSSPLTPTFASSMCLLAPCYLTGDSVRDKCVEMLSAAL-----KADDDYKDYGVN 245

Query: 247 SDPIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLIN 306
            D  ++A  +E  +++    +    + + RS + NLKDP+NP  RR VL G I    +  
Sbjct: 246 CD--KMASEIEDHIYQELKSTDMKYRNRVRSRISNLKDPRNPGLRRNVLSGAISAGLIAK 305

Query: 307 LSTTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSAD 366
           ++  +MASD+ +     + Q+A+ + +       TTD F+C +C ++  TY Q+QTRSAD
Sbjct: 306 MTAEEMASDELRELRNAMTQEAIREHQMAKTGGTTTDLFQCSKCKKKNCTYNQVQTRSAD 348

Query: 367 EPMTTFVTCVNCNNHWKFC 378
           EPMTTFV C  C N WKFC
Sbjct: 366 EPMTTFVLCNECGNRWKFC 348

[Close]

BLAST of Lcy05g021100 vs. ExPASy TrEMBL
Match: A0A6J1GYA3 (transcription elongation factor TFIIS-like OS=Cucurbita moschata OX=3662 GN=LOC111458597 PE=4 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 2.9e-189
Identity = 352/377 (93.37%), Postives = 362/377 (96.02%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVSYQILVSTQVGKRLR 60
           ME ELVELFEAAKKAADAAAA SNDGG EESRCLDALRQLKKFPV+YQILVSTQVGKRLR
Sbjct: 1   MEKELVELFEAAKKAADAAAARSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEK 120
           HLTKHP+KKIQDYASDLIEMWKDIVIKETNKNKKNGNGN+  KEP KIGSPSAESVKVE 
Sbjct: 61  HLTKHPKKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNS--KEPSKIGSPSAESVKVEN 120

Query: 121 FQKSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEE 180
            QKSSSMKVE+VSKVE FDRNG+TSSVKFSRS SA+SEKNSVKVEKTDSVVKVER+VKEE
Sbjct: 121 LQKSSSMKVERVSKVEQFDRNGSTSSVKFSRSASAVSEKNSVKVEKTDSVVKVERMVKEE 180

Query: 181 KKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNASD 240
           KKISIEKKPS GAA PPKLTSMIKSKDAARDKIRELL EAFSKVPGEA EDVIDEVNASD
Sbjct: 181 KKISIEKKPS-GAAGPPKLTSMIKSKDAARDKIRELLVEAFSKVPGEADEDVIDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLS 300
           PIRVAVSVESVMFENWGGSTGAQKAKYRS+MFNLKDPKNPDFRRKVLLGLIKPER+ NLS
Sbjct: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMTNLS 300

Query: 301 TTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           TTDMASDQRKRENEEIAQKALF+CERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TTDMASDQRKRENEEIAQKALFECERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 374

[Close]

BLAST of Lcy05g021100 vs. ExPASy TrEMBL
Match: A0A6J1JLK7 (transcription elongation factor TFIIS-like OS=Cucurbita maxima OX=3661 GN=LOC111486982 PE=4 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 2.4e-188
Identity = 351/377 (93.10%), Postives = 362/377 (96.02%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVSYQILVSTQVGKRLR 60
           ME ELVELFEAAKKAADAAAA SNDGG EESRCLDALRQLKKFPV+YQILVSTQVGKRLR
Sbjct: 1   MEKELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEK 120
           HLTKHP+KKIQDYASDLIEMWKDIVIKETNKNKKNGNGN+  KEP KIGSPSAESVKVE 
Sbjct: 61  HLTKHPKKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNS--KEPSKIGSPSAESVKVEN 120

Query: 121 FQKSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEE 180
           FQKSSSMKVE+VSKVE F+R G+TSSVKFSRS SA+SEKNSVKVEKTDSVVKVER+VKEE
Sbjct: 121 FQKSSSMKVERVSKVEQFNRIGSTSSVKFSRSASAVSEKNSVKVEKTDSVVKVERMVKEE 180

Query: 181 KKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNASD 240
           KKISIEKKPS GAA PPKLTSMIKSKDAARDKIRELL EAFSKVPGEA EDVIDEVNASD
Sbjct: 181 KKISIEKKPS-GAAGPPKLTSMIKSKDAARDKIRELLVEAFSKVPGEADEDVIDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLS 300
           PIRVAVSVESVMFENWGGSTGAQKAKYRS+MFNLKDPKNPDFRRKVLLGLIKPER+ NLS
Sbjct: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMTNLS 300

Query: 301 TTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           TTDMASDQRKRENEEIAQKALF+CERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TTDMASDQRKRENEEIAQKALFECERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 374

[Close]

BLAST of Lcy05g021100 vs. ExPASy TrEMBL
Match: A0A6J1BWY6 (transcription elongation factor TFIIS OS=Momordica charantia OX=3673 GN=LOC111006416 PE=4 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 5.1e-186
Identity = 344/379 (90.77%), Postives = 358/379 (94.46%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVSYQILVSTQVGKRLR 60
           MENELVELFEAAKKAADAAAA SNDGG EESRCLDALRQLKKFPV+YQILVSTQVGKRLR
Sbjct: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEK 120
           HLTKHP+KKIQDYASDLIEMWK+IVIKETNKNKKNGNG  NCKE PKIGSPSAESVKVEK
Sbjct: 61  HLTKHPKKKIQDYASDLIEMWKEIVIKETNKNKKNGNG--NCKESPKIGSPSAESVKVEK 120

Query: 121 FQKSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSV--VKVERIVK 180
           FQKSSSMKVE+VSKVE  DRNG TSSVK+SRSES ISEKN VKVEKTDS+  VK ER+VK
Sbjct: 121 FQKSSSMKVERVSKVEQSDRNGTTSSVKYSRSESVISEKNIVKVEKTDSLEKVKAERVVK 180

Query: 181 EEKKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNA 240
           EEKKISI+K+PS G AAPPKLTSMIKSKDAARDKIRELL EAFSKVPGEA EDVIDEVNA
Sbjct: 181 EEKKISIDKRPSSGTAAPPKLTSMIKSKDAARDKIRELLCEAFSKVPGEADEDVIDEVNA 240

Query: 241 SDPIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLIN 300
           SDP+RVAVSVESVMFENWGGSTGAQKAKYRS+MFNLKDPKNPDFRR VL+G IKPERLIN
Sbjct: 241 SDPVRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRNVLVGHIKPERLIN 300

Query: 301 LSTTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSAD 360
           +ST +MASDQRKRENEEI QKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSAD
Sbjct: 301 MSTAEMASDQRKRENEEIEQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSAD 360

Query: 361 EPMTTFVTCVNCNNHWKFC 378
           EPMTTFVTCVNCNNHWKFC
Sbjct: 361 EPMTTFVTCVNCNNHWKFC 377

[Close]

BLAST of Lcy05g021100 vs. ExPASy TrEMBL
Match: A0A0A0KDH9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G014610 PE=4 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 1.6e-184
Identity = 340/377 (90.19%), Postives = 359/377 (95.23%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVSYQILVSTQVGKRLR 60
           MENELVELFEAAKKAADAAAA SNDGG EESRCLDALRQLKKFPV+YQILVSTQVGKRLR
Sbjct: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEK 120
           HLTKHP+KKIQ++ASDLIEMWK+IVIKETNKNKK  NGNA+ K+ PKIGSPSAESVKVEK
Sbjct: 61  HLTKHPKKKIQEHASDLIEMWKEIVIKETNKNKK--NGNASSKDSPKIGSPSAESVKVEK 120

Query: 121 FQKSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEE 180
           FQKSSSMKVE+VSKVE FDRNGATSSVK+S+SES +SE+NSVKVEKTDS+VKVER+VKE 
Sbjct: 121 FQKSSSMKVERVSKVEQFDRNGATSSVKYSKSESVVSERNSVKVEKTDSMVKVERVVKE- 180

Query: 181 KKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNASD 240
                EKKPS GAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEA E+ +DEVNASD
Sbjct: 181 -----EKKPSSGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEEFMDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLS 300
           PIRVAVSVESVMFENWGGSTGAQKAKYRS+MFNLKDPKNPDFRRKVLLGLIKPER+IN+S
Sbjct: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMINMS 300

Query: 301 TTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           T DMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TADMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 369

[Close]

BLAST of Lcy05g021100 vs. ExPASy TrEMBL
Match: A0A6J1FF11 (transcription elongation factor TFIIS-like OS=Cucurbita moschata OX=3662 GN=LOC111444888 PE=4 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 1.6e-184
Identity = 340/377 (90.19%), Postives = 356/377 (94.43%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVSYQILVSTQVGKRLR 60
           MENELVELFEAAKKAADAAAA SNDGG EESRCLDALRQLKKFPV+YQILVSTQVGKRLR
Sbjct: 1   MENELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEK 120
           HLTKHP+KKIQ+YASDLIE+WK+IVIK+TNKNKK  NGN +C+E PKIGSPSAESVKVEK
Sbjct: 61  HLTKHPKKKIQNYASDLIEIWKEIVIKDTNKNKK--NGNIDCEESPKIGSPSAESVKVEK 120

Query: 121 FQKSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEE 180
           F+KSSSMKVE+VS+VE FDRNG TSS KFSRSES IS KN VKVEKTDSVVKVERIVKEE
Sbjct: 121 FRKSSSMKVERVSQVEQFDRNGVTSSAKFSRSESDISGKNPVKVEKTDSVVKVERIVKEE 180

Query: 181 KKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNASD 240
           K+ISIEKKPS G  APPKLTSMIKSKDAARDKIRELLFEAFSKVP EA EDV+DEVNASD
Sbjct: 181 KRISIEKKPSSGPDAPPKLTSMIKSKDAARDKIRELLFEAFSKVPSEADEDVMDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLS 300
           PIRVAVSVESVMFENWGGSTG+QK KYRS+MFNLKDPKNPDFRRKVLLGLIKPERLIN+S
Sbjct: 241 PIRVAVSVESVMFENWGGSTGSQKTKYRSIMFNLKDPKNPDFRRKVLLGLIKPERLINMS 300

Query: 301 TTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           T DMASDQRKRENEEI QKALFDCER GAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TADMASDQRKRENEEIEQKALFDCERAGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 375

[Close]

BLAST of Lcy05g021100 vs. NCBI nr
Match: XP_038884917.1 (transcription elongation factor TFIIS [Benincasa hispida])

HSP 1 Score: 671.8 bits (1732), Expect = 3.5e-189
Identity = 344/377 (91.25%), Postives = 363/377 (96.29%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVSYQILVSTQVGKRLR 60
           MENELVELF+AAKKAADAAAA SNDGG EESRCLDALRQLKKFPV+YQILVSTQVGKRLR
Sbjct: 1   MENELVELFQAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEK 120
           HLTKHP+KKIQ+YASDL+EMWK+IVIKETNKNKKNGNG  NCK+ PKIGSPSAESVKVEK
Sbjct: 61  HLTKHPKKKIQEYASDLVEMWKEIVIKETNKNKKNGNG--NCKDSPKIGSPSAESVKVEK 120

Query: 121 FQKSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEE 180
           FQKSSSMKVE+VSKVE FD+NGAT SVK+SR ESA+SE+NSVKVEKTDS VKVE  VKEE
Sbjct: 121 FQKSSSMKVERVSKVEQFDKNGATGSVKYSRLESAVSERNSVKVEKTDSAVKVEGTVKEE 180

Query: 181 KKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNASD 240
           ++ISI+KKPSGG AAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEA EDV+DEVNASD
Sbjct: 181 RRISIDKKPSGGTAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEADEDVMDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLS 300
           PIRVAVSVESVMFENWGGSTGAQKAKYRS+MFNLKDPKNPDFRRKVLLGLIKPER+IN+S
Sbjct: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMINMS 300

Query: 301 TTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           T DMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TADMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 375

[Close]

BLAST of Lcy05g021100 vs. NCBI nr
Match: XP_022957122.1 (transcription elongation factor TFIIS-like [Cucurbita moschata])

HSP 1 Score: 671.0 bits (1730), Expect = 6.0e-189
Identity = 352/377 (93.37%), Postives = 362/377 (96.02%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVSYQILVSTQVGKRLR 60
           ME ELVELFEAAKKAADAAAA SNDGG EESRCLDALRQLKKFPV+YQILVSTQVGKRLR
Sbjct: 1   MEKELVELFEAAKKAADAAAARSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEK 120
           HLTKHP+KKIQDYASDLIEMWKDIVIKETNKNKKNGNGN+  KEP KIGSPSAESVKVE 
Sbjct: 61  HLTKHPKKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNS--KEPSKIGSPSAESVKVEN 120

Query: 121 FQKSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEE 180
            QKSSSMKVE+VSKVE FDRNG+TSSVKFSRS SA+SEKNSVKVEKTDSVVKVER+VKEE
Sbjct: 121 LQKSSSMKVERVSKVEQFDRNGSTSSVKFSRSASAVSEKNSVKVEKTDSVVKVERMVKEE 180

Query: 181 KKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNASD 240
           KKISIEKKPS GAA PPKLTSMIKSKDAARDKIRELL EAFSKVPGEA EDVIDEVNASD
Sbjct: 181 KKISIEKKPS-GAAGPPKLTSMIKSKDAARDKIRELLVEAFSKVPGEADEDVIDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLS 300
           PIRVAVSVESVMFENWGGSTGAQKAKYRS+MFNLKDPKNPDFRRKVLLGLIKPER+ NLS
Sbjct: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMTNLS 300

Query: 301 TTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           TTDMASDQRKRENEEIAQKALF+CERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TTDMASDQRKRENEEIAQKALFECERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 374

[Close]

BLAST of Lcy05g021100 vs. NCBI nr
Match: KAG6602582.1 (Transcription elongation factor TFIIS, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 669.8 bits (1727), Expect = 1.3e-188
Identity = 351/377 (93.10%), Postives = 362/377 (96.02%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVSYQILVSTQVGKRLR 60
           ME ELVELFEAAKKAADAAAA SNDGG EESRCLDALRQLKKFPV+YQILVSTQVGKRLR
Sbjct: 300 MEKELVELFEAAKKAADAAAARSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 359

Query: 61  HLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEK 120
           HLTKHP+KKIQDYASDLIEMWKDIVIKETNKNKKNGNGN+  KEP KIGSPSAESVKVE 
Sbjct: 360 HLTKHPKKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNS--KEPSKIGSPSAESVKVEN 419

Query: 121 FQKSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEE 180
            QKSSSMKVE+VSKVE FDRNG+TSSVKFSRS SA+SEKNSVKVEKTDSVVKVERIVKEE
Sbjct: 420 LQKSSSMKVERVSKVEQFDRNGSTSSVKFSRSASAVSEKNSVKVEKTDSVVKVERIVKEE 479

Query: 181 KKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNASD 240
           KKISIEKKPS GAA PPKLT+MIKSKDAARDKIRELL EAFSKVPGEA EDV+DEVNASD
Sbjct: 480 KKISIEKKPS-GAAGPPKLTAMIKSKDAARDKIRELLVEAFSKVPGEADEDVMDEVNASD 539

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLS 300
           PIRVAVSVESVMFENWGGSTGAQKAKYRS+MFNLKDPKNPDFRRKVLLGLIKPER+ NLS
Sbjct: 540 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMTNLS 599

Query: 301 TTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           TTDMASDQRKRENEEIAQKALF+CERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 600 TTDMASDQRKRENEEIAQKALFECERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 659

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 660 MTTFVTCVNCNNHWKFC 673

[Close]

BLAST of Lcy05g021100 vs. NCBI nr
Match: XP_022989946.1 (transcription elongation factor TFIIS-like [Cucurbita maxima])

HSP 1 Score: 667.9 bits (1722), Expect = 5.1e-188
Identity = 351/377 (93.10%), Postives = 362/377 (96.02%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVSYQILVSTQVGKRLR 60
           ME ELVELFEAAKKAADAAAA SNDGG EESRCLDALRQLKKFPV+YQILVSTQVGKRLR
Sbjct: 1   MEKELVELFEAAKKAADAAAAPSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEK 120
           HLTKHP+KKIQDYASDLIEMWKDIVIKETNKNKKNGNGN+  KEP KIGSPSAESVKVE 
Sbjct: 61  HLTKHPKKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNS--KEPSKIGSPSAESVKVEN 120

Query: 121 FQKSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEE 180
           FQKSSSMKVE+VSKVE F+R G+TSSVKFSRS SA+SEKNSVKVEKTDSVVKVER+VKEE
Sbjct: 121 FQKSSSMKVERVSKVEQFNRIGSTSSVKFSRSASAVSEKNSVKVEKTDSVVKVERMVKEE 180

Query: 181 KKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNASD 240
           KKISIEKKPS GAA PPKLTSMIKSKDAARDKIRELL EAFSKVPGEA EDVIDEVNASD
Sbjct: 181 KKISIEKKPS-GAAGPPKLTSMIKSKDAARDKIRELLVEAFSKVPGEADEDVIDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLS 300
           PIRVAVSVESVMFENWGGSTGAQKAKYRS+MFNLKDPKNPDFRRKVLLGLIKPER+ NLS
Sbjct: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMTNLS 300

Query: 301 TTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           TTDMASDQRKRENEEIAQKALF+CERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TTDMASDQRKRENEEIAQKALFECERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 374

[Close]

BLAST of Lcy05g021100 vs. NCBI nr
Match: KAG7033260.1 (Transcription elongation factor TFIIS, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 667.5 bits (1721), Expect = 6.6e-188
Identity = 350/377 (92.84%), Postives = 361/377 (95.76%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAASSNDGGVEESRCLDALRQLKKFPVSYQILVSTQVGKRLR 60
           ME ELVELFEAAKKAADAAAA SNDGG EESRCLDALRQLKKFPV+YQILVSTQVGKRLR
Sbjct: 1   MEKELVELFEAAKKAADAAAARSNDGGAEESRCLDALRQLKKFPVTYQILVSTQVGKRLR 60

Query: 61  HLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAESVKVEK 120
           HLTKHP+KKIQDYASDLIEMWKDIVIKETNKNKKNGNG++  KEP KIGSPSAESVKVE 
Sbjct: 61  HLTKHPKKKIQDYASDLIEMWKDIVIKETNKNKKNGNGDS--KEPSKIGSPSAESVKVEN 120

Query: 121 FQKSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKVERIVKEE 180
            QKSSSMK E+VSKVE FDRNG+TSSVKFSRS SA+SEKNSVKVEKTDSVVKVERIVKEE
Sbjct: 121 LQKSSSMKAERVSKVEQFDRNGSTSSVKFSRSASAVSEKNSVKVEKTDSVVKVERIVKEE 180

Query: 181 KKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDEVNASD 240
           KKISIEKKPS GAA PPKLTSMIKSKDAARDKIRELL EAFSKVPGEA EDV+DEVNASD
Sbjct: 181 KKISIEKKPS-GAAGPPKLTSMIKSKDAARDKIRELLVEAFSKVPGEADEDVMDEVNASD 240

Query: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLS 300
           PIRVAVSVESVMFENWGGSTGAQKAKYRS+MFNLKDPKNPDFRRKVLLGLIKPER+ NLS
Sbjct: 241 PIRVAVSVESVMFENWGGSTGAQKAKYRSIMFNLKDPKNPDFRRKVLLGLIKPERMTNLS 300

Query: 301 TTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360
           TTDMASDQRKRENEEIAQKALF+CERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP
Sbjct: 301 TTDMASDQRKRENEEIAQKALFECERGGAPKATTDQFKCGRCGQRKTTYYQLQTRSADEP 360

Query: 361 MTTFVTCVNCNNHWKFC 378
           MTTFVTCVNCNNHWKFC
Sbjct: 361 MTTFVTCVNCNNHWKFC 374

[Close]

BLAST of Lcy05g021100 vs. TAIR 10
Match: AT2G38560.1 (transcript elongation factor IIS )

HSP 1 Score: 392.5 bits (1007), Expect = 3.8e-109
Identity = 221/384 (57.55%), Postives = 269/384 (70.05%), Query Frame = 0


Query: 1   MENELVELFEAAKKAADAAAASS-NDGGVEESRCLDALRQLKKFPVSYQILVSTQVGKRL 60
           ME++L++LFE AKKAADAAA       G E S+C+DAL+QLKKFPV+Y  LV+TQVGK+L
Sbjct: 1   MESDLIDLFEGAKKAADAAALDGVTSSGPEVSQCIDALKQLKKFPVTYDTLVATQVGKKL 60

Query: 61  RHLTKHPRKKIQDYASDLIEMWKDIVIKETNKNKKNGNGNANCKEPPKIGSPSAES---- 120
           R L KHP + I+  A+DL+E+WK +VI+ET K KK   G   CKE  K+     E     
Sbjct: 61  RSLAKHPVEDIKSVATDLLEIWKKVVIEETAKAKKT-EGTNGCKE-AKVNKMDVEKPSNP 120

Query: 121 --VKVEKFQKSSSMKVEKVSKVEHFDRNGATSSVKFSRSESAISEKNSVKVEKTDSVVKV 180
             VKV+K Q+  S K  KV + E    N   + VK  R    I   N  K++     VK 
Sbjct: 121 APVKVQKLQRGDSAKSIKVERKE--PDNKVVTGVKIERKVPDIKVTNGTKIDYRGQAVKD 180

Query: 181 ERIVKEEKKISIEKKPSGGAAAPPKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVI 240
           E++ K+ +  S  K P+  A APPKLT+M+K  D  RDKIRELL EA  +V GEA +   
Sbjct: 181 EKVSKDNQ--SSMKAPAKAANAPPKLTAMLKCNDPVRDKIRELLVEALCRVAGEADDYER 240

Query: 241 DEVNASDPIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKP 300
           + VNASDP+RVAVSVES+MFE  G STGAQK KYRS+MFNL+D  NPD RR+VL G I P
Sbjct: 241 ESVNASDPLRVAVSVESLMFEKLGRSTGAQKLKYRSIMFNLRDSNNPDLRRRVLTGEISP 300

Query: 301 ERLINLSTTDMASDQRKRENEEIAQKALFDCERGGAPKATTDQFKCGRCGQRKTTYYQLQ 360
           E+LI LS  DMASD+RK+EN +I +KALFDCERG A KA+TDQFKCGRCGQRK TYYQ+Q
Sbjct: 301 EKLITLSAEDMASDKRKQENNQIKEKALFDCERGLAAKASTDQFKCGRCGQRKCTYYQMQ 360

Query: 361 TRSADEPMTTFVTCVNCNNHWKFC 378
           TRSADEPMTT+VTCVNC+NHWKFC
Sbjct: 361 TRSADEPMTTYVTCVNCDNHWKFC 378

[Close]

BLAST of Lcy05g021100 vs. TAIR 10
Match: AT2G42730.1 (F-box family protein )

HSP 1 Score: 100.9 bits (250), Expect = 2.3e-21
Identity = 57/127 (44.88%), Postives = 79/127 (62.20%), Query Frame = 0


Query: 197 PKLTSMIKSKDAARDKIRELLFEAFSKVPGE-AGEDVIDEVNASDPIRVAVSVESVMFEN 256
           P  ++M K+ D+ RDK+RE+L  +  KV  E    ++   V A DP  VAVSVES MFE 
Sbjct: 594 PTHSTMKKTGDSKRDKVREILQTSLVKVASEIVDTEMKTRVTACDPSVVAVSVESAMFEK 653

Query: 257 WGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLSTTDMASDQRKRENEE 316
            G   G  KAKYRS++FN+ D  NPD RRKVL+G I  ERL+ +   +M S++ ++E + 
Sbjct: 654 LGCFMGPHKAKYRSILFNMGDSNNPDLRRKVLIGEINGERLVTMERQEMGSEKIQKEVQR 713

Query: 317 IAQKALF 323
           I + A F
Sbjct: 714 IKENARF 720

[Close]

BLAST of Lcy05g021100 vs. TAIR 10
Match: AT4G18720.1 (Transcription factor IIS protein )

HSP 1 Score: 98.6 bits (244), Expect = 1.1e-20
Identity = 55/111 (49.55%), Postives = 72/111 (64.86%), Query Frame = 0


Query: 197 PKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGE-DVIDEVNASDPIRVAVSVESVMFEN 256
           P   +M K+ D+ RDK+RE+L  + +KV  E  + ++   V A DP  VAVSVE+ MFEN
Sbjct: 105 PTHATMKKTGDSKRDKVREILQTSLAKVASEVVDTEMKTRVTACDPWVVAVSVETAMFEN 164

Query: 257 WGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLSTTDMAS 307
            G   G QKAKYRS++FN+ D  NPD RRKVLLG I  ERL+ +   +M S
Sbjct: 165 LGCFMGPQKAKYRSILFNMGDSNNPDLRRKVLLGEISGERLVKMEKEEMGS 215

[Close]

BLAST of Lcy05g021100 vs. TAIR 10
Match: AT5G42325.1 (Transcription factor IIS protein )

HSP 1 Score: 70.9 bits (172), Expect = 2.5e-12
Identity = 45/128 (35.16%), Postives = 70/128 (54.69%), Query Frame = 0


Query: 197 PKLTSMIKSKDAARDKIRELLFEAFSKVPGEAGEDVIDE--VNASDPIRVAVSVESVMFE 256
           P  ++M K+ D+ RDK+ E+L  + +KV  E  +  +    +   DP  VAVSVES M  
Sbjct: 104 PTHSTMKKTGDSKRDKVHEILQSSLAKVATEVVDTEMKRRVMTVCDPWVVAVSVESAM-- 163

Query: 257 NWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINLSTTDMASDQRKRENE 316
                         S++FN+ D  NPD RRKVL+G I  ERL+ +   +M S++ ++E +
Sbjct: 164 --------------SILFNMGDSNNPDLRRKVLIGEISGERLVKMEKDEMGSEKIQKEVQ 215

Query: 317 EIAQKALF 323
            I ++A F
Sbjct: 224 RIKERARF 215

[Close]

BLAST of Lcy05g021100 vs. TAIR 10
Match: AT5G25520.1 (SPOC domain / Transcription elongation factor S-II protein )

HSP 1 Score: 61.6 bits (148), Expect = 1.5e-09
Identity = 33/83 (39.76%), Postives = 51/83 (61.45%), Query Frame = 0


Query: 240 DPIRVAVSVESVMFENWGGSTGAQKAKYRSVMFNLKDPKNPDFRRKVLLGLIKPERLINL 299
           DP  +A  +E  +F+ +GG     K K RS++FNLKD  NP+ R  V+ G I PERL N+
Sbjct: 359 DPELLASKIELELFKLFGGVNKKYKEKGRSLLFNLKDKNNPELRESVMSGKISPERLCNM 418

Query: 300 STTDMASDQ----RKRENEEIAQ 319
           +  ++AS +    R+ + EE+A+
Sbjct: 419 TAEELASKELSQWRQAKAEEMAE 441

The following BLAST results are available for this feature:

BLAST of Lcy05g021100 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZVH8	5.4e-108	57.55	Transcription elongation factor TFIIS OS=Arabidopsis thaliana OX=3702 GN=TFIIS P...	[more]
P20232	5.0e-29	29.14	Transcription elongation factor S-II OS=Drosophila melanogaster OX=7227 GN=TfIIS...	[more]
Q2KI09	9.4e-28	28.95	Transcription elongation factor A protein 3 OS=Bos taurus OX=9913 GN=TCEA3 PE=2 ...	[more]
P23881	6.1e-27	29.06	Transcription elongation factor A protein 3 OS=Mus musculus OX=10090 GN=Tcea3 PE...	[more]
O75764	6.7e-26	29.55	Transcription elongation factor A protein 3 OS=Homo sapiens OX=9606 GN=TCEA3 PE=...	[more]

BLAST of Lcy05g021100 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GYA3	2.9e-189	93.37	transcription elongation factor TFIIS-like OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1JLK7	2.4e-188	93.10	transcription elongation factor TFIIS-like OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1BWY6	5.1e-186	90.77	transcription elongation factor TFIIS OS=Momordica charantia OX=3673 GN=LOC11100...	[more]
A0A0A0KDH9	1.6e-184	90.19	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G014610 PE=4 SV=1	[more]
A0A6J1FF11	1.6e-184	90.19	transcription elongation factor TFIIS-like OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]

BLAST of Lcy05g021100 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038884917.1	3.5e-189	91.25	transcription elongation factor TFIIS [Benincasa hispida]	[more]
XP_022957122.1	6.0e-189	93.37	transcription elongation factor TFIIS-like [Cucurbita moschata]	[more]
KAG6602582.1	1.3e-188	93.10	Transcription elongation factor TFIIS, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022989946.1	5.1e-188	93.10	transcription elongation factor TFIIS-like [Cucurbita maxima]	[more]
KAG7033260.1	6.6e-188	92.84	Transcription elongation factor TFIIS, partial [Cucurbita argyrosperma subsp. ar...	[more]

BLAST of Lcy05g021100 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38560.1	3.8e-109	57.55	transcript elongation factor IIS	[more]
AT2G42730.1	2.3e-21	44.88	F-box family protein	[more]
AT4G18720.1	1.1e-20	49.55	Transcription factor IIS protein	[more]
AT5G42325.1	2.5e-12	35.16	Transcription factor IIS protein	[more]
AT5G25520.1	1.5e-09	39.76	SPOC domain / Transcription elongation factor S-II protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003617	Transcription elongation factor, TFIIS/CRSP70, N-terminal, sub-type	SMART	SM00509	TFS2_5	coord: 8..87 e-value: 6.7E-23 score: 92.1
IPR003618	Transcription elongation factor S-II, central domain	SMART	SM00510	mid_6	coord: 207..315 e-value: 5.5E-35 score: 132.2
IPR003618	Transcription elongation factor S-II, central domain	PFAM	PF07500	TFIIS_M	coord: 207..323 e-value: 8.3E-31 score: 106.8
IPR003618	Transcription elongation factor S-II, central domain	PROSITE	PS51321	TFIIS_CENTRAL	coord: 209..332 score: 34.197464
IPR001222	Zinc finger, TFIIS-type	SMART	SM00440	Cys4_2	coord: 337..376 e-value: 9.7E-24 score: 94.9
IPR001222	Zinc finger, TFIIS-type	PFAM	PF01096	TFIIS_C	coord: 337..375 e-value: 1.1E-19 score: 70.0
IPR001222	Zinc finger, TFIIS-type	PROSITE	PS00466	ZF_TFIIS_1	coord: 339..374
IPR001222	Zinc finger, TFIIS-type	PROSITE	PS51133	ZF_TFIIS_2	coord: 335..375 score: 14.636859
None	No IPR available	GENE3D	2.20.25.10		coord: 306..377 e-value: 1.4E-27 score: 97.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 91..115
None	No IPR available	PANTHER	PTHR11477	TRANSCRIPTION FACTOR S-II ZINC FINGER DOMAIN-CONTAINING PROTEIN	coord: 1..377
None	No IPR available	PANTHER	PTHR11477:SF36	TRANSCRIPTION ELONGATION FACTOR TFIIS	coord: 1..377
None	No IPR available	CDD	cd00183	TFIIS_I	coord: 22..85 e-value: 1.39373E-15 score: 69.2594
None	No IPR available	CDD	cd13749	Zn-ribbon_TFIIS	coord: 330..376 e-value: 3.36612E-30 score: 108.077
None	No IPR available	SUPERFAMILY	57783	Zinc beta-ribbon	coord: 318..375
IPR017923	Transcription factor IIS, N-terminal	PFAM	PF08711	Med26	coord: 36..85 e-value: 1.9E-10 score: 40.6
IPR017923	Transcription factor IIS, N-terminal	PROSITE	PS51319	TFIIS_N	coord: 10..88 score: 20.377293
IPR035100	Transcription elongation factor, IIS-type	PIRSF	PIRSF006704	TFIIS	coord: 2..377 e-value: 3.4E-97 score: 323.6
IPR036575	Transcription elongation factor S-II, central domain superfamily	GENE3D	1.10.472.30		coord: 192..305 e-value: 2.3E-25 score: 91.0
IPR036575	Transcription elongation factor S-II, central domain superfamily	SUPERFAMILY	46942	Elongation factor TFIIS domain 2	coord: 196..307
IPR006289	Transcription elongation factor, TFIIS	TIGRFAM	TIGR01385	TIGR01385	coord: 12..377 e-value: 1.9E-97 score: 324.5
IPR035441	TFIIS/LEDGF domain superfamily	GENE3D	1.20.930.10	Conserved domain common to transcription factors TFIIS, elongin A, CRSP70	coord: 1..101 e-value: 3.9E-23 score: 83.0
IPR035441	TFIIS/LEDGF domain superfamily	SUPERFAMILY	47676	Conserved domain common to transcription factors TFIIS, elongin A, CRSP70	coord: 29..98

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy05g021100.1	Lcy05g021100.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0006414	translational elongation
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003746	translation elongation factor activity
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0003676	nucleic acid binding