Homology
BLAST of Lcy05g009630 vs. ExPASy Swiss-Prot
Match:
Q969N2 (GPI transamidase component PIG-T OS=Homo sapiens OX=9606 GN=PIGT PE=1 SV=1)
HSP 1 Score: 294.3 bits (752), Expect = 3.5e-78
Identity = 197/594 (33.16%), Postives = 296/594 (49.83%), Query Frame = 0
Query: 25 VSEGAEEDFSEDLLLKPLPDRNVLAHFHFQSRAPSSRSNSYGRHHHVFPKAISQLVHKYR 84
++E + E+L++ PLP +V A F F++R S H+ +FPKA+ QL+ KY
Sbjct: 22 LAEPPRDSLREELVITPLPSGDVAATFQFRTRWDSELQREGVSHYRLFPKALGQLISKYS 81
Query: 85 IKEMELSFTQGRWRYDRWGGFDTISSSNAKPSGVELWAVFDVPPNEVDASWKNLTHSLSG 144
++E+ LSFTQG WR WG PSG ELW F +VD SWK L++ LSG
Sbjct: 82 LRELHLSFTQGFWRTRYWG-----PPFLQAPSGAELWVWFQDTVTDVDKSWKELSNVLSG 141
Query: 145 LFCASINFLESSTSYSAPKWGFHP---ASEN----MRYGTLPREAVCTENLTPWLKLLPC 204
+FCAS+NF++ ST+ P F P A++ +RY LPRE VCTENLTPW KLLPC
Sbjct: 142 IFCASLNFID-STNTVTPTASFKPLGLANDTDHYFLRYAVLPREVVCTENLTPWKKLLPC 201
Query: 205 RDKSGLSVLMDRPSIYKGFYHSQRLHLLSSEFDSNAVDSAIVLDQTLTVVLQPYSHRGTL 264
K+GLSVL+ ++ YHSQ +H+ ++ + L QTL+VV +
Sbjct: 202 SSKAGLSVLLKADRLFHTSYHSQAVHIRPVCRNARCTSISWELRQTLSVVFDAF------ 261
Query: 265 SYSTATQLQPSWSLSSIFGRRVTGRCALARSSNVYIQLDRGLMAELQGMLGEQEILAITR 324
Q + WSL +F R +T C LA S VY+ +
Sbjct: 262 ---ITGQGKKDWSLFRMFSRTLTEPCPLASESRVYVDI---------------------- 321
Query: 325 TSSEGSRSNGAFELSANPDRVHMEISSRDDKHLSVLYMFIVEEKYDNSEPLDLRFTWKIP 384
+ ++ N E+ P + ++ K ++ Y + +NS L+++ WK P
Sbjct: 322 --TTYNQDNETLEVHPPPTTTYQDVILGTRKTYAI-YDLLDTAMINNSRNLNIQLKWKRP 381
Query: 385 VFWSIPQAP-LHVTRFLLGSGNERGAIALQLKSTKPSDRLMPDTVIKDSCSLLVRVFQVV 444
P P LH R++ G G ++G ++ L +T P R P V + V
Sbjct: 382 PENEAPPVPFLHAQRYVSGYGLQKGELSTLLYNTHPY-RAFP-----------VLLLDTV 441
Query: 445 PWYIKVYYHTLQVFIDNQPHTIKNVVEKMQVSPSKDKVSPGVMEMLLKFPCGLKSAALTI 504
PWY+++Y HTL + + +N + P++D++ P ++EML++ P ++I
Sbjct: 442 PWYLRLYVHTLTITSKGK----ENKPSYIHYQPAQDRLQPHLLEMLIQLPAN-SVTKVSI 501
Query: 505 EFDKGFLHIDEYPPDANQGLDIPSAVIS--FPDFSTSTQFVENNSSKSPILSKLQGQSP- 564
+F++ L EY PD N G + +V+S P + + +SP+ + L S
Sbjct: 502 QFERALLKWTEYTPDPNHGFYVSPSVLSALVPSMVAAKPV---DWEESPLFNSLFPVSDG 555
Query: 565 ----ILSYTEVLLVPLTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVGEEE 604
+ YTE LLV L TPDFSMPYNVI +TCTV+A+ +GS N+L R EE
Sbjct: 562 SNYFVRLYTEPLLVNLPTPDFSMPYNVICLTCTVVAVCYGSFYNLLTRTFHIEE 555
BLAST of Lcy05g009630 vs. ExPASy Swiss-Prot
Match:
Q8BXQ2 (GPI transamidase component PIG-T OS=Mus musculus OX=10090 GN=Pigt PE=1 SV=2)
HSP 1 Score: 290.0 bits (741), Expect = 6.7e-77
Identity = 202/611 (33.06%), Postives = 304/611 (49.75%), Query Frame = 0
Query: 8 ISLLCLPILFAVSAIGSVSEGAEEDFSEDLLLKPLPDRNVLAHFHFQSRAPSSRSNSYGR 67
+ L L +L +EG + E+L++ PLP +V A F F++R S
Sbjct: 9 LPLRLLVLLLVGRGCCGCAEGPRDSLREELVITPLPSGDVAATFQFRTRWDSDLQREGVS 68
Query: 68 HHHVFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDTISSSNAKPSGVELWAVFDVP 127
H+ +FPKA+ QL+ KY ++E+ LSFTQG WR WG PSG ELW F
Sbjct: 69 HYRLFPKALGQLISKYSLRELHLSFTQGFWRTRYWG-----PPFLQAPSGAELWVWFQDT 128
Query: 128 PNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHP---ASEN----MRYGTLPR 187
+VD SW+ L++ LSG+FCAS+NF++ +T+ P F P A++ +RY LPR
Sbjct: 129 VTDVDKSWRELSNVLSGIFCASLNFID-ATNTVTPTASFKPLGLANDTDDYFLRYAVLPR 188
Query: 188 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSSEFDSNAVDSAIVL 247
E VCTENLTPW KLLPC K+GLSVL+ ++ YHSQ +H+ +++ + L
Sbjct: 189 EVVCTENLTPWKKLLPCSSKAGLSVLLKADRLFHTSYHSQAVHIRPICRNAHCTSISWEL 248
Query: 248 DQTLTVVLQPYSHRGTLSYSTATQLQPSWSLSSIFGRRVTGRCALARSSNVYIQLDRGLM 307
QTL+VV + Q + WSL +F R +T C LA S VY+ +
Sbjct: 249 RQTLSVVFDAF---------ITGQGKKDWSLFRMFSRTLTEACPLASQSLVYVDI----- 308
Query: 308 AELQGMLGEQEILAITRTSSEGSRSNGAFELSANPDRVHMEISSRDDKHLSVLYMFIVEE 367
+ S+ N E+S P + ++ K +V +F
Sbjct: 309 -------------------TGYSQDNETLEVSPPPTSTYQDVILGTRKTYAVYDLFDT-A 368
Query: 368 KYDNSEPLDLRFTWKIPV-FWSIPQAPLHVTRFLLGSGNERGAIALQLKSTKPSDRLMPD 427
+NS L+++ WK P ++P LH R++ G G ++G ++ L ++ P R P
Sbjct: 369 MINNSRNLNIQLKWKRPPDNEALPVPFLHAQRYVSGYGLQKGELSTLLYNSHPY-RAFP- 428
Query: 428 TVIKDSCSLLVRVFQVVPWYIKVYYHTLQVFIDNQPHTIKNVVEKMQVSPSKDKVSPGVM 487
V + VVPWY+++Y HTL + + +N + P++D+ P ++
Sbjct: 429 ----------VLLLDVVPWYLRLYVHTLTITSKGK----ENKPSYIHYQPAQDRQQPHLL 488
Query: 488 EMLLKFPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVIS--FPDFSTSTQFVEN 547
EML++ P ++I+F++ L EY PD N G + +V+S P +
Sbjct: 489 EMLIQLPAN-SVTKVSIQFERALLKWTEYTPDPNHGFYVSPSVLSALVPSVVAAKPV--- 548
Query: 548 NSSKSPILSKL----QGQSPILS-YTEVLLVPLTTPDFSMPYNVITITCTVLALYFGSLL 604
+ SP+ + L G S + YTE LLV L TPDFSMPYNVI +TCTV+A+ +GS
Sbjct: 549 DWEGSPLFNTLFPVSDGSSYFVRLYTEPLLVNLPTPDFSMPYNVICLTCTVVAVCYGSFY 559
BLAST of Lcy05g009630 vs. ExPASy Swiss-Prot
Match:
P38875 (GPI transamidase component GPI16 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=GPI16 PE=1 SV=2)
HSP 1 Score: 204.1 bits (518), Expect = 4.8e-51
Identity = 183/634 (28.86%), Postives = 291/634 (45.90%), Query Frame = 0
Query: 19 VSAIGSVSEGAEEDFSEDLLLKPLPDRNVLAHFHFQSRA----PSSRSNSYGRHHH--VF 78
VS IG +++ + E L+LKPLP+ ++L F FQ ++ P+ S SY + H F
Sbjct: 23 VSQIG-INDSLWYPYDEALVLKPLPNNDLLLSFAFQLQSEPFDPAVSSMSYDAYEHYTTF 82
Query: 79 PKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDTISSSNAKPSGVELWA-VFDVPPNEV 138
P+AI L+ ++ L FT+G W WG + A SGVELW+ V + +
Sbjct: 83 PRAIPPLLESTATRQFHLRFTRGFWDALSWGQLPH-AGKEAGASGVELWSQVQAMDQEQA 142
Query: 139 DASWKNLTHSLSGLFCASINFLESS----------TSYSAPKWGFHPASENMRYGTLPRE 198
+WK L++SLSGLFC+S+NF++ S + AP + MR +LP E
Sbjct: 143 FHNWKKLSNSLSGLFCSSLNFIDESRTTFPRRSYASDIGAPLFNSTEKLYLMR-ASLPNE 202
Query: 199 AVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSSEFDSNAVDSAIVLD 258
+CTENLTP++KLLP R KSGL+ L+D ++ ++S L + + + +D
Sbjct: 203 PICTENLTPFIKLLPTRGKSGLTSLLDGHKLFDSLWNSISLDIATICSEDEDALCHYEMD 262
Query: 259 QTLTVVLQPYSHRGTLSYSTATQLQPSWSLSSIFGRRVTGRC-ALARSSNVYIQLDRGLM 318
+ +V S L + + +C L S + +L +
Sbjct: 263 ARIEMVTHVPSALARGERPIPKPLDGNTLRCDTDKPFDSYQCFPLPEPSQTHFKLSQLFA 322
Query: 319 AELQGMLGEQEILAITRTSSEGSRSNGAFELSANPDRVHMEIS----SRDDKHLSVLYMF 378
+ G TR +E RS LS + + S D Y F
Sbjct: 323 RPINN--GNLFANRPTRICAEVDRSTWTAFLSVDDTIFSTHDNCFDLSNDQNEGGSGYDF 382
Query: 379 IVEEKYDNSEPLDLRFTWKIPVFWSIPQAPLHVTRFLLGSGNERGAIALQLKSTKPSDRL 438
I+E D + + T +PV P+HV+R L G+G +RG + + +
Sbjct: 383 ILEST-DTT-----KVTPIVPV-------PIHVSRSLTGNGQDRGGMRIVFHNDN----- 442
Query: 439 MPDTVIKDSCSLLVRVFQVVPWYIKVYYHTLQVFIDNQPHTIKN--VVEKMQVSPSKDKV 498
DT +K + F+ +PW+++VY +LQ+ P +N +++K + + D+
Sbjct: 443 --DTPVK------LIYFESLPWFMRVYLSSLQITSTTSPQLQENDIILDKYYLQ-AADRK 502
Query: 499 SPGVMEMLLKFPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVISFPDFSTSTQF 558
PG +E + P +T +FDK L EYPPDAN G +I +AVI+ +S+
Sbjct: 503 RPGHLEFTMLIPAN-TDIVMTYQFDKALLQFAEYPPDANHGFEIDAAVITVLSLESSSSL 562
Query: 559 VENNSSKSPILSKLQGQSPILSYTEVLLVPLTTPDFSMPYNVITITCTVLALYFGSLLNV 618
E T LL+ L+TPDFSMPYNVI +T T++ L FG L N+
Sbjct: 563 YEMR-------------------TSTLLLSLSTPDFSMPYNVIILTSTIMGLIFGMLYNL 604
Query: 619 LRRRV--GEEERFMKAKATKKFQVPQLVSKLLAK 627
+ +R+ EE + ++ K+++ +L K L K
Sbjct: 623 MVKRMVTVEEADKITLQSGLKYKLLKLKEKFLGK 604
BLAST of Lcy05g009630 vs. ExPASy Swiss-Prot
Match:
O94380 (GPI transamidase component PIG-T homolog OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gpi16 PE=3 SV=1)
HSP 1 Score: 194.5 bits (493), Expect = 3.8e-48
Identity = 181/634 (28.55%), Postives = 292/634 (46.06%), Query Frame = 0
Query: 12 CLPILFAVSAIGSVSEGA--EEDFSEDLLLKPLPDRNVLAHFHFQSRAPSSRSNS---YG 71
CL +LFA S + A +E + E L +K R F F+ A + ++S
Sbjct: 6 CL-LLFAYSLLSFSLTAATIDETYDESLFIKSFSSRYSYVSFAFEIGASTDSTHSSVFSE 65
Query: 72 RHHHVFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDTISSSNAKPS---GVELWAV 131
+FP +I++++ + ++ E+ + T+GRW Y+ W S N S G E+WA
Sbjct: 66 SSFSLFPLSIARVMDECQVSELHIRATRGRWDYENW----KESPDNGFYSGGLGFEVWAF 125
Query: 132 FDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSY----SAPKWGFHPASENMRY-GTL 191
P+ W LT+ LSGL CAS+N+++SS +Y S P G S N +Y +L
Sbjct: 126 MANDPSM--KYWLKLTNQLSGLLCASLNYIDSSNTYQPQLSYP--GSFSFSNNTQYFASL 185
Query: 192 PREAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSSEFDSNAVDSAI 251
P+E VCTENL+P KLLPC+ K+G++ L+D + +HS + + SE S A
Sbjct: 186 PQEDVCTENLSPLFKLLPCKRKAGIASLLDSHLFFDTDWHSFSIDVYPSENQSLASVKMG 245
Query: 252 VLDQTLTVVLQPYSHRGTLSYSTATQLQPSWSLSSIFGRRVTGRCALARSSNVYIQLDRG 311
++ Q + V + +G ++ ++ + S+ C ++ S + +D
Sbjct: 246 IIIQAVVDVERNGRRKGKTTFQPPSEYCHDEDMDSL-------HCLMSGYSTEHHTVD-- 305
Query: 312 LMAELQGMLGEQEILAITRTSSEGSRSNGAFELSANPDRVHMEISSRDDKHLSVLYMFIV 371
+L + ++ L ++ T S+ SNG
Sbjct: 306 ---DLFHKVPKERCL-LSSTFSDVFVSNG------------------------------- 365
Query: 372 EEKYDNSEPLDLRFTWKIPVFWSIPQAPLHVTRFLLGSGNERGAIALQLKSTKPSDRLMP 431
+K D LD +IP+ + + V R L GN G+ L ST + P
Sbjct: 366 -DKIDTFS-LDEAANIQIPIQSTSDNHTVTVDRSLSNDGNHWGS----LSSTIYNPSSSP 425
Query: 432 DTVIKDSCSLLVRVFQVVPWYIKVYYHTLQVFIDNQPHTIKNVVEKMQVSPSKDKVSPGV 491
T++ F+ PW+++VY HTL + ++ K+ +EK+ P +D+ + +
Sbjct: 426 RTIV---------YFEKFPWFVRVYLHTLTITLNGTRINTKDFIEKLYYQPLRDRKAGTM 485
Query: 492 MEMLLKFPCGLKSAALTIEF--DKGFLHIDEYPPDANQGLDIPSAVISFPDFSTSTQFVE 551
ME+ P L + F +K L +DEYPPDAN+G ++P A+IS F E
Sbjct: 486 MEIQFSIP---PHTNLIVHFNVEKTPLRLDEYPPDANRGYNLPPAIISV--------FDE 545
Query: 552 NNSSKSPILSKLQGQSPILSYTEVLLVPLTTPDFSMPYNVITITCTVLALYFGSLLNVLR 611
NN+ L L+ T LL+ + TPDFSMPYNVI T TV+AL FG + N+L
Sbjct: 546 NNTK----LCSLR--------TAALLMFIPTPDFSMPYNVIIFTSTVIALTFGGIFNLLT 545
Query: 612 RR-VGEEERFMKAKATKKFQVPQLVSKLLAKLRG 630
RR V ++ +F + + + +L K+ K RG
Sbjct: 606 RRFVPQQSKFQNRQPS---MLQRLKEKIFHKKRG 545
BLAST of Lcy05g009630 vs. ExPASy TrEMBL
Match:
A0A6J1GMR1 (GPI transamidase component GPI16 OS=Cucurbita moschata OX=3662 GN=LOC111455925 PE=4 SV=1)
HSP 1 Score: 1239.9 bits (3207), Expect = 0.0e+00
Identity = 621/669 (92.83%), Postives = 642/669 (95.96%), Query Frame = 0
Query: 1 MASFLRLISLLCLPILFAVSAIGSVSEGAEEDFSEDLLLKPLPDRNVLAHFHFQSRAPSS 60
MASFLRLISLLCLPILFAV AIGSVSEG EEDFSEDLLLKPLPDR VLAHFHFQS APSS
Sbjct: 1 MASFLRLISLLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSGAPSS 60
Query: 61 RSNSYGRHHHVFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDTISSSNAKPSGVEL 120
RSNSYGRHHH+FPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFD+ISSSNAKPSGVEL
Sbjct: 61 RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL 120
Query: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180
WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR
Sbjct: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180
Query: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSSEFDSNAVDSAIVL 240
EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLS+EFDSNAV+SAIVL
Sbjct: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVNSAIVL 240
Query: 241 DQTLTVVLQPYSHRGTLSYSTATQLQPSWSLSSIFGRRVTGRCALARSSNVYIQLDRGLM 300
DQTLTVVLQPYSHRG L YS A QLQPSWSLSSIFGR+V GRC LA SSNVYIQLD+GLM
Sbjct: 241 DQTLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLASSSNVYIQLDKGLM 300
Query: 301 AELQGMLGEQEILAITRTSSEGSRSNGAFELSANPDRVHMEISSRDDKHLSVLYMFIVEE 360
AELQGMLGEQE+LA+TR+SSEGS N AFELSANPDRVHME+SSRDDKHLSVLYMF V E
Sbjct: 301 AELQGMLGEQEMLAVTRSSSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSVAE 360
Query: 361 KYDNSEPLDLRFTWKIPVFWSIPQAPLHVTRFLLGSGNERGAIALQLKSTKPSDRLMPDT 420
KYD SEPLDLRFTWKIPV WSIPQAPLHVTRF+LGSGNERGAIALQLKSTKPSD+L+PDT
Sbjct: 361 KYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPDT 420
Query: 421 VIKDSCSLLVRVFQVVPWYIKVYYHTLQVFIDNQPHTIKNVVEKMQVSPSKDKVSPGVME 480
VI DSCSLLV+VFQVVPWYIKVYYHTLQVFID+QPH+I NVVEKMQVSPS+DKVSPGV+E
Sbjct: 421 VITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLE 480
Query: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVISFPDFSTSTQFVENNSS 540
MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDA+QGLDIPSAVI+FPDF TSTQF ENNSS
Sbjct: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSS 540
Query: 541 KSPILSKLQGQSPILSYTEVLLVPLTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
KSPILSK QGQSPILSYTEVLLVP TTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG
Sbjct: 541 KSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
Query: 601 EEERFMKAKATKKFQVPQLVSKLLAKLRGRHWEPSESQAPSSASNSKSKLLFKVILVAGL 660
EEERFMK KA KKFQV QLVSKLLAKLRGRHWEPSESQAPSS+SNSKSKLLFKVILVAGL
Sbjct: 601 EEERFMKDKAKKKFQVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKSKLLFKVILVAGL 660
Query: 661 AAAWQYYFG 670
AAAWQYYFG
Sbjct: 661 AAAWQYYFG 669
BLAST of Lcy05g009630 vs. ExPASy TrEMBL
Match:
A0A6J1JL56 (GPI transamidase component GPI16 OS=Cucurbita maxima OX=3661 GN=LOC111487934 PE=4 SV=1)
HSP 1 Score: 1234.2 bits (3192), Expect = 0.0e+00
Identity = 618/669 (92.38%), Postives = 640/669 (95.67%), Query Frame = 0
Query: 1 MASFLRLISLLCLPILFAVSAIGSVSEGAEEDFSEDLLLKPLPDRNVLAHFHFQSRAPSS 60
M SFLRLISLLCLPILFAV AIGSVSEG EEDFSEDLLLKPLPDR VLAHFHFQS APSS
Sbjct: 1 MVSFLRLISLLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSVAPSS 60
Query: 61 RSNSYGRHHHVFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDTISSSNAKPSGVEL 120
RSNSYGRHHH+FPKAISQLVHKYRIKEMELSFTQGRW YDRWGGFD+ISSSNAKPSGVEL
Sbjct: 61 RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWHYDRWGGFDSISSSNAKPSGVEL 120
Query: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180
WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGF PASENMRYGTLPR
Sbjct: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFRPASENMRYGTLPR 180
Query: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSSEFDSNAVDSAIVL 240
EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLS+EFDSNAVDSAIVL
Sbjct: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVL 240
Query: 241 DQTLTVVLQPYSHRGTLSYSTATQLQPSWSLSSIFGRRVTGRCALARSSNVYIQLDRGLM 300
DQTLTVVLQPYSHRG L YS A QLQPSWSLSSIFGR+V GRC LARSSNVYIQLD+GLM
Sbjct: 241 DQTLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGLM 300
Query: 301 AELQGMLGEQEILAITRTSSEGSRSNGAFELSANPDRVHMEISSRDDKHLSVLYMFIVEE 360
AELQGMLGEQE+LA+TR+SSEGS N AFELSANPDRVHME+SSR+DKHLSVLYMF V E
Sbjct: 301 AELQGMLGEQEMLAVTRSSSEGSTRNLAFELSANPDRVHMEMSSRNDKHLSVLYMFSVAE 360
Query: 361 KYDNSEPLDLRFTWKIPVFWSIPQAPLHVTRFLLGSGNERGAIALQLKSTKPSDRLMPDT 420
KYD SEPLDLRFTWKIPV WSIPQAPLHVTRF+LGSGNERGAIALQLKSTKPSD+L+P+T
Sbjct: 361 KYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPNT 420
Query: 421 VIKDSCSLLVRVFQVVPWYIKVYYHTLQVFIDNQPHTIKNVVEKMQVSPSKDKVSPGVME 480
VI DSCSLLV+VFQVVPWYIKVYYHTLQVFID+QPH+I NVVEKMQVSPS+DKVSPGV+E
Sbjct: 421 VITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLE 480
Query: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVISFPDFSTSTQFVENNSS 540
MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDA+QGLDIPSAVI+FPDF TSTQF ENNSS
Sbjct: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSS 540
Query: 541 KSPILSKLQGQSPILSYTEVLLVPLTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
KSPILSK QGQSPILSYTEVLLVP TTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG
Sbjct: 541 KSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
Query: 601 EEERFMKAKATKKFQVPQLVSKLLAKLRGRHWEPSESQAPSSASNSKSKLLFKVILVAGL 660
EEERFMK KA KKFQV QLVSKLLAKLRGRHWEPSESQAPSS+SNSKSKLLFKVILVAGL
Sbjct: 601 EEERFMKDKAKKKFQVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKSKLLFKVILVAGL 660
Query: 661 AAAWQYYFG 670
AAAWQYYFG
Sbjct: 661 AAAWQYYFG 669
BLAST of Lcy05g009630 vs. ExPASy TrEMBL
Match:
A0A0A0K6R2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G340200 PE=4 SV=1)
HSP 1 Score: 1221.5 bits (3159), Expect = 0.0e+00
Identity = 611/669 (91.33%), Postives = 634/669 (94.77%), Query Frame = 0
Query: 1 MASFLRLISLLCLPILFAVSAIGSVSEGAEEDFSEDLLLKPLPDRNVLAHFHFQSRAPSS 60
MASFLRLISLLCLPILFAVS GSVSEG EEDFSEDLLLKPLPDR VLAHFHFQSRAPSS
Sbjct: 1 MASFLRLISLLCLPILFAVSVNGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSS 60
Query: 61 RSNSYGRHHHVFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDTISSSNAKPSGVEL 120
RSNSYGRHHH+FPKAISQLVHKYRIKEMELSFTQGRWRYD WGGFD ISSSNAKPSGVEL
Sbjct: 61 RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDHWGGFDLISSSNAKPSGVEL 120
Query: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180
WAVFDV PNEVDA+WKNLTHSLSGLFCASINFLESSTSYSAPKW FHP SENMRYGTLPR
Sbjct: 121 WAVFDVLPNEVDATWKNLTHSLSGLFCASINFLESSTSYSAPKWSFHPESENMRYGTLPR 180
Query: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSSEFDSNAVDSAIVL 240
EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKG+YHSQRLHLLSSEFDSNAVDSAIVL
Sbjct: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGYYHSQRLHLLSSEFDSNAVDSAIVL 240
Query: 241 DQTLTVVLQPYSHRGTLSYSTATQLQPSWSLSSIFGRRVTGRCALARSSNVYIQLDRGLM 300
DQTLTVVLQP++HRGTL YSTATQLQPSWSLS+IFGRRV G+C+LARSSNVYIQLDRGLM
Sbjct: 241 DQTLTVVLQPHNHRGTLGYSTATQLQPSWSLSTIFGRRVIGKCSLARSSNVYIQLDRGLM 300
Query: 301 AELQGMLGEQEILAITRTSSEGSRSNGAFELSANPDRVHMEISSRDDKHLSVLYMFIVEE 360
AELQGMLGEQE+ +I R EGSRSN AFELSANPDRVHME+SSR DKH SVLYMF+V E
Sbjct: 301 AELQGMLGEQEMFSIARAGFEGSRSNPAFELSANPDRVHMEMSSRYDKHASVLYMFMVAE 360
Query: 361 KYDNSEPLDLRFTWKIPVFWSIPQAPLHVTRFLLGSGNERGAIALQLKSTKPSDRLMPDT 420
KYD+SEPLDLRFTWKIPV WSIPQAPLHVTRFLLGSGNERGAIALQLKSTKPSDRLMPDT
Sbjct: 361 KYDDSEPLDLRFTWKIPVAWSIPQAPLHVTRFLLGSGNERGAIALQLKSTKPSDRLMPDT 420
Query: 421 VIKDSCSLLVRVFQVVPWYIKVYYHTLQVFIDNQPHTIKNVVEKMQVSPSKDKVSPGVME 480
V D+CSLLVRVFQVVPWYIKVYYHTL +FI++QPH I NV+EKMQVSPSKDKVSPGVME
Sbjct: 421 VFADNCSLLVRVFQVVPWYIKVYYHTLHIFINDQPHKINNVIEKMQVSPSKDKVSPGVME 480
Query: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVISFPDFSTSTQFVENNSS 540
MLLK PCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVISFPDFSTSTQFVENNSS
Sbjct: 481 MLLKLPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVISFPDFSTSTQFVENNSS 540
Query: 541 KSPILSKLQGQSPILSYTEVLLVPLTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
KSPIL K QGQ+PILSYTEVLLVP TTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG
Sbjct: 541 KSPILLKWQGQNPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
Query: 601 EEERFMKAKATKKFQVPQLVSKLLAKLRGRHWEPSESQAPSSASNSKSKLLFKVILVAGL 660
EEERFMK+KAT+K +V LVSKLLAKLRGR WEPSESQ+PSS SNSKSKLLFKVILVAGL
Sbjct: 601 EEERFMKSKATRKLRVLHLVSKLLAKLRGRQWEPSESQSPSSVSNSKSKLLFKVILVAGL 660
Query: 661 AAAWQYYFG 670
AAAWQYYFG
Sbjct: 661 AAAWQYYFG 669
BLAST of Lcy05g009630 vs. ExPASy TrEMBL
Match:
A0A1S3C6Z6 (GPI transamidase component PIG-T OS=Cucumis melo OX=3656 GN=LOC103497563 PE=4 SV=1)
HSP 1 Score: 1210.7 bits (3131), Expect = 0.0e+00
Identity = 608/669 (90.88%), Postives = 633/669 (94.62%), Query Frame = 0
Query: 1 MASFLRLISLLCLPILFAVSAIGSVSEGAEEDFSEDLLLKPLPDRNVLAHFHFQSRAPSS 60
MASFLRLISLLCLPILFAVS IGSVSEGAEEDFSEDLLLKPLPDR VLAHFHFQS APSS
Sbjct: 1 MASFLRLISLLCLPILFAVSVIGSVSEGAEEDFSEDLLLKPLPDRKVLAHFHFQSTAPSS 60
Query: 61 RSNSYGRHHHVFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDTISSSNAKPSGVEL 120
RSNSYGRHHH+FPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFD+ISSSNAKPSGVEL
Sbjct: 61 RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL 120
Query: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180
WAVFDVPPNEVDA+WKNLTHSLSGLFCASINFLESSTSYSAPKW FHPASENMRYGTLPR
Sbjct: 121 WAVFDVPPNEVDATWKNLTHSLSGLFCASINFLESSTSYSAPKWSFHPASENMRYGTLPR 180
Query: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSSEFDSNAVDSAIVL 240
EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKG+YHSQRLHLLSSEFDS+ VDSAIVL
Sbjct: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGYYHSQRLHLLSSEFDSDGVDSAIVL 240
Query: 241 DQTLTVVLQPYSHRGTLSYSTATQLQPSWSLSSIFGRRVTGRCALARSSNVYIQLDRGLM 300
DQTLTVVLQP+SHRGTL YS+ATQLQPSWSLSSIFGRRV G+C+LARSSNVYIQLDRGLM
Sbjct: 241 DQTLTVVLQPHSHRGTLGYSSATQLQPSWSLSSIFGRRVIGKCSLARSSNVYIQLDRGLM 300
Query: 301 AELQGMLGEQEILAITRTSSEGSRSNGAFELSANPDRVHMEISSRDDKHLSVLYMFIVEE 360
AELQG+LGEQE AI R SSEGS SN AFELSANPDRVHME+S+ DKH+SVLYMF+V
Sbjct: 301 AELQGILGEQEKFAIARASSEGSISNPAFELSANPDRVHMEMSNGYDKHISVLYMFMVAA 360
Query: 361 KYDNSEPLDLRFTWKIPVFWSIPQAPLHVTRFLLGSGNERGAIALQLKSTKPSDRLMPDT 420
KYD+SEPLDLRFTWKIP+ WSIPQAPL+VTRFLLGSGNERG IALQLKSTKP D+LMPDT
Sbjct: 361 KYDDSEPLDLRFTWKIPIAWSIPQAPLYVTRFLLGSGNERGVIALQLKSTKPRDQLMPDT 420
Query: 421 VIKDSCSLLVRVFQVVPWYIKVYYHTLQVFIDNQPHTIKNVVEKMQVSPSKDKVSPGVME 480
V DSCSLLVRVFQVVPWYIKVYYHTLQVFID+Q H I NVVEKMQVSPSKDKVSPGVME
Sbjct: 421 VFADSCSLLVRVFQVVPWYIKVYYHTLQVFIDDQRHIITNVVEKMQVSPSKDKVSPGVME 480
Query: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVISFPDFSTSTQFVENNSS 540
MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVISFPDF TSTQFVE NSS
Sbjct: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVISFPDFFTSTQFVEKNSS 540
Query: 541 KSPILSKLQGQSPILSYTEVLLVPLTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
KSPIL K QG++PILSYTEVLLVP TTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG
Sbjct: 541 KSPILLKWQGKNPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
Query: 601 EEERFMKAKATKKFQVPQLVSKLLAKLRGRHWEPSESQAPSSASNSKSKLLFKVILVAGL 660
EEERFMK+KAT+K +V LVSKLLAKLRGR WEPSESQ+ SS SNSKSKLLFKVILVAGL
Sbjct: 601 EEERFMKSKATRKLRVSHLVSKLLAKLRGRQWEPSESQSHSSVSNSKSKLLFKVILVAGL 660
Query: 661 AAAWQYYFG 670
AAAWQYYFG
Sbjct: 661 AAAWQYYFG 669
BLAST of Lcy05g009630 vs. ExPASy TrEMBL
Match:
A0A6J1DDU7 (GPI transamidase component PIG-T OS=Momordica charantia OX=3673 GN=LOC111019616 PE=4 SV=1)
HSP 1 Score: 1208.4 bits (3125), Expect = 0.0e+00
Identity = 608/670 (90.75%), Postives = 638/670 (95.22%), Query Frame = 0
Query: 1 MASFLRLISLLCLPILFAVSAIGSVSEGAEEDFSEDLLLKPLPDRNVLAHFHFQSRAPSS 60
MASFLRLI+LLCLPILFAVSAIGSVSEG+EEDFSEDLLLKPLPDR VLAHFHFQSRAPSS
Sbjct: 1 MASFLRLIALLCLPILFAVSAIGSVSEGSEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSS 60
Query: 61 RSNSYGRHHHVFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDTISSSNAKPSGVEL 120
RSNSYGRHHH+FPKAISQLV+KYRIKEMELSFTQGRWRYDRWGGFD+ISSSNAKPSGVEL
Sbjct: 61 RSNSYGRHHHLFPKAISQLVYKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL 120
Query: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180
WAVFDVP N+VDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFH ASENMRYGTLPR
Sbjct: 121 WAVFDVPSNDVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHSASENMRYGTLPR 180
Query: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSSEFDSNAVDSAIVL 240
EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHL SSEFDSNAVDSAIVL
Sbjct: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLRSSEFDSNAVDSAIVL 240
Query: 241 DQTLTVVLQPYSHRGTLSYSTATQLQPSWSLSSIFGRRVTGRCALARSSNVYIQLDRGLM 300
DQTLTVVLQP+SHRGT S STAT+LQPSWSLSSIFGR+VTGRCALA+SSNVYI LDRGLM
Sbjct: 241 DQTLTVVLQPHSHRGTFSPSTATRLQPSWSLSSIFGRKVTGRCALAKSSNVYIHLDRGLM 300
Query: 301 AELQGMLGEQEILAITRTSSEGSRSNGAFELSANPDRVHMEISSRDDKHLSVLYMFIVEE 360
AELQGML EQEILAITR+SSEG RSN AFELSANPDRVHMEI+SRDDK S++YMF+VEE
Sbjct: 301 AELQGMLREQEILAITRSSSEGFRSNPAFELSANPDRVHMEINSRDDKPSSIIYMFMVEE 360
Query: 361 KYDNSEPLDLRFTWKIPVFWSIPQAPLHVTRFLLGSGNERGAIALQLKSTKPSDRLMPDT 420
KYD+SEPLDLRFTWKI V WSIPQAPLHVTRFLLGSGNERGAIALQLKSTKPSDRLMPDT
Sbjct: 361 KYDDSEPLDLRFTWKISVVWSIPQAPLHVTRFLLGSGNERGAIALQLKSTKPSDRLMPDT 420
Query: 421 VIKDSCSLLVRVFQVVPWYIKVYYHTLQVFIDNQPHTIKNVVEKMQVSPSKDKVSPGVME 480
VI DSCSLLV+VFQVVPWYIKVYYHTLQVFID+QP+ I NVVEKMQVSPSKDKVSPGVME
Sbjct: 421 VITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPNAITNVVEKMQVSPSKDKVSPGVME 480
Query: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVISFPDFSTSTQFVENNSS 540
+LLKFPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVI+FPDF STQ ENNSS
Sbjct: 481 VLLKFPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVITFPDFHASTQLTENNSS 540
Query: 541 KSPILSKLQGQSPILSYTEVLLVPLTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
KSPIL KLQ QSPILSYTEVLLVPLTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG
Sbjct: 541 KSPILLKLQDQSPILSYTEVLLVPLTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
Query: 601 EEERFMKAKATKKFQVPQLVSKLLAKLRGRHWEPSESQAPSSASN-SKSKLLFKVILVAG 660
EEERF+K+KA+KK Q+ Q+ SKLLAKLRGRHWEPSE +A SS+S+ SKL+FKVILV G
Sbjct: 601 EEERFLKSKASKKLQISQIASKLLAKLRGRHWEPSEPEAQSSSSSFFNSKLVFKVILVGG 660
Query: 661 LAAAWQYYFG 670
LAAAWQYYFG
Sbjct: 661 LAAAWQYYFG 670
BLAST of Lcy05g009630 vs. NCBI nr
Match:
KAG7014511.1 (GPI transamidase component PIG-T, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1246.9 bits (3225), Expect = 0.0e+00
Identity = 624/669 (93.27%), Postives = 644/669 (96.26%), Query Frame = 0
Query: 1 MASFLRLISLLCLPILFAVSAIGSVSEGAEEDFSEDLLLKPLPDRNVLAHFHFQSRAPSS 60
MASFLRLISLLCLPILFAV AIGSVSEG EEDFSEDLLLKPLPDR VLAHFHFQSRAPSS
Sbjct: 1 MASFLRLISLLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSS 60
Query: 61 RSNSYGRHHHVFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDTISSSNAKPSGVEL 120
RSNSYGRHHH+FPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFD+ISSSNAKPSGVEL
Sbjct: 61 RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL 120
Query: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180
WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR
Sbjct: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180
Query: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSSEFDSNAVDSAIVL 240
EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLS+EFDSNAVDSAIVL
Sbjct: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVL 240
Query: 241 DQTLTVVLQPYSHRGTLSYSTATQLQPSWSLSSIFGRRVTGRCALARSSNVYIQLDRGLM 300
DQTLTVVLQPYSHRG L YS A QLQPSWSLSSIFGR+V GRC LARSSNVYIQLD+GLM
Sbjct: 241 DQTLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGLM 300
Query: 301 AELQGMLGEQEILAITRTSSEGSRSNGAFELSANPDRVHMEISSRDDKHLSVLYMFIVEE 360
AELQGMLGEQE+LA+TR+SSEGS N AFELSANPDRVHME+SSRDDKHLSVLYMF V E
Sbjct: 301 AELQGMLGEQEMLAVTRSSSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSVAE 360
Query: 361 KYDNSEPLDLRFTWKIPVFWSIPQAPLHVTRFLLGSGNERGAIALQLKSTKPSDRLMPDT 420
KYD SEPLDLRFTWKIPV WSIPQAPLHVTRF+LGSGNERGAIALQLKSTKPSD+L+PDT
Sbjct: 361 KYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPDT 420
Query: 421 VIKDSCSLLVRVFQVVPWYIKVYYHTLQVFIDNQPHTIKNVVEKMQVSPSKDKVSPGVME 480
VI DSCSLLV+VFQVVPWYIKVYYHTLQVFID+QPH+I NVVEKMQVSPS+DKVSPGV+E
Sbjct: 421 VITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLE 480
Query: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVISFPDFSTSTQFVENNSS 540
MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDA+QGLDIPSAVI+FPDF TSTQF ENNSS
Sbjct: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSS 540
Query: 541 KSPILSKLQGQSPILSYTEVLLVPLTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
KSPILSK QGQSPILSYTEVLLVP TTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG
Sbjct: 541 KSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
Query: 601 EEERFMKAKATKKFQVPQLVSKLLAKLRGRHWEPSESQAPSSASNSKSKLLFKVILVAGL 660
EEERFMK KA KKFQV QLVSKLLAKLRGRHWEPSESQAPSS+SNSKSKLLFKVILVAGL
Sbjct: 601 EEERFMKDKAKKKFQVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKSKLLFKVILVAGL 660
Query: 661 AAAWQYYFG 670
AAAWQYYFG
Sbjct: 661 AAAWQYYFG 669
BLAST of Lcy05g009630 vs. NCBI nr
Match:
KAG6575986.1 (GPI transamidase component PIG-T, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1245.3 bits (3221), Expect = 0.0e+00
Identity = 623/669 (93.12%), Postives = 643/669 (96.11%), Query Frame = 0
Query: 1 MASFLRLISLLCLPILFAVSAIGSVSEGAEEDFSEDLLLKPLPDRNVLAHFHFQSRAPSS 60
MASFLRLISLLCLPILFAV AIGSVSEG EEDFSEDLLLKPLPDR VLAHFHFQSRAPSS
Sbjct: 89 MASFLRLISLLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSS 148
Query: 61 RSNSYGRHHHVFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDTISSSNAKPSGVEL 120
RSNSYGRHHH+FPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFD+ISSSNAKPSGVEL
Sbjct: 149 RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL 208
Query: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180
WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR
Sbjct: 209 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 268
Query: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSSEFDSNAVDSAIVL 240
EAVCTENLTPWLKLLPCRDKSG SVLMDRPSIYKGFYHSQRLHLLS+EFDSNAVDSAIVL
Sbjct: 269 EAVCTENLTPWLKLLPCRDKSGFSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVL 328
Query: 241 DQTLTVVLQPYSHRGTLSYSTATQLQPSWSLSSIFGRRVTGRCALARSSNVYIQLDRGLM 300
DQTLTVVLQPYSHRG L YS A QLQPSWSLSSIFGR+V GRC LARSSNVYIQLD+GLM
Sbjct: 329 DQTLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGLM 388
Query: 301 AELQGMLGEQEILAITRTSSEGSRSNGAFELSANPDRVHMEISSRDDKHLSVLYMFIVEE 360
AELQGMLGEQE+LA+TR+SSEGS N AFELSANPDRVHME+SSRDDKHLSVLYMF V E
Sbjct: 389 AELQGMLGEQEMLAVTRSSSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSVAE 448
Query: 361 KYDNSEPLDLRFTWKIPVFWSIPQAPLHVTRFLLGSGNERGAIALQLKSTKPSDRLMPDT 420
KYD SEPLDLRFTWKIPV WSIPQAPLHVTRF+LGSGNERGAIALQLKSTKPSD+L+PDT
Sbjct: 449 KYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPDT 508
Query: 421 VIKDSCSLLVRVFQVVPWYIKVYYHTLQVFIDNQPHTIKNVVEKMQVSPSKDKVSPGVME 480
VI DSCSLLV+VFQVVPWYIKVYYHTLQVFID+QPH+I NVVEKMQVSPS+DKVSPGV+E
Sbjct: 509 VITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLE 568
Query: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVISFPDFSTSTQFVENNSS 540
MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDA+QGLDIPSAVI+FPDF TSTQF ENNSS
Sbjct: 569 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSS 628
Query: 541 KSPILSKLQGQSPILSYTEVLLVPLTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
KSPILSK QGQSPILSYTEVLLVP TTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG
Sbjct: 629 KSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 688
Query: 601 EEERFMKAKATKKFQVPQLVSKLLAKLRGRHWEPSESQAPSSASNSKSKLLFKVILVAGL 660
EEERFMK KA KKFQV QLVSKLLAKLRGRHWEPSESQAPSS+SNSKSKLLFKVILVAGL
Sbjct: 689 EEERFMKDKAKKKFQVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKSKLLFKVILVAGL 748
Query: 661 AAAWQYYFG 670
AAAWQYYFG
Sbjct: 749 AAAWQYYFG 757
BLAST of Lcy05g009630 vs. NCBI nr
Match:
XP_023548556.1 (GPI transamidase component PIG-T isoform X1 [Cucurbita pepo subsp. pepo] >XP_023548557.1 GPI transamidase component PIG-T isoform X2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1241.9 bits (3212), Expect = 0.0e+00
Identity = 622/669 (92.97%), Postives = 643/669 (96.11%), Query Frame = 0
Query: 1 MASFLRLISLLCLPILFAVSAIGSVSEGAEEDFSEDLLLKPLPDRNVLAHFHFQSRAPSS 60
MASFLRLISLLCLPILFAV AIGSVSEG EEDFSEDLLLKPLPDR VLAHFHFQSRAPSS
Sbjct: 1 MASFLRLISLLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSRAPSS 60
Query: 61 RSNSYGRHHHVFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDTISSSNAKPSGVEL 120
RSNSYGRHHH+FPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFD+ISSSNAKPSGVEL
Sbjct: 61 RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL 120
Query: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180
WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGF PASENMRYGTLPR
Sbjct: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFRPASENMRYGTLPR 180
Query: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSSEFDSNAVDSAIVL 240
EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLS+EFDSNAVDSAIVL
Sbjct: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVL 240
Query: 241 DQTLTVVLQPYSHRGTLSYSTATQLQPSWSLSSIFGRRVTGRCALARSSNVYIQLDRGLM 300
DQTLTVVLQPYSHRG L YS A QLQPSWSLSSIFGR+V GRC LARSSNVYIQLD+GLM
Sbjct: 241 DQTLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGLM 300
Query: 301 AELQGMLGEQEILAITRTSSEGSRSNGAFELSANPDRVHMEISSRDDKHLSVLYMFIVEE 360
AELQGMLGEQE+LA+TR+SSEGS N AFELSANPDRVHME+SSRDDKHLSVLYMF V E
Sbjct: 301 AELQGMLGEQEMLAVTRSSSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSVAE 360
Query: 361 KYDNSEPLDLRFTWKIPVFWSIPQAPLHVTRFLLGSGNERGAIALQLKSTKPSDRLMPDT 420
KYD SEPLDLRFTWKIPV WSIPQAPLHVTRF+LGSGNERGAIALQLKSTKPSD+L+P+T
Sbjct: 361 KYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPNT 420
Query: 421 VIKDSCSLLVRVFQVVPWYIKVYYHTLQVFIDNQPHTIKNVVEKMQVSPSKDKVSPGVME 480
VI DSCSLLV+VFQVVPWYIKVYYHTLQVFID+QPH+I NVVEKMQVSPS+DKVSPGV+E
Sbjct: 421 VITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLE 480
Query: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVISFPDFSTSTQFVENNSS 540
MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDA+QGLDIPSAVI+FPDF TSTQF ENNSS
Sbjct: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSS 540
Query: 541 KSPILSKLQGQSPILSYTEVLLVPLTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
KSPILSK QGQSPILSYTEVLLVP TTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG
Sbjct: 541 KSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
Query: 601 EEERFMKAKATKKFQVPQLVSKLLAKLRGRHWEPSESQAPSSASNSKSKLLFKVILVAGL 660
EEERFMK KA KKFQV QLVSKLLAKLRGRHWEPSESQAPSS+SNSKSKLLFKVILVAGL
Sbjct: 601 EEERFMKDKAKKKFQVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKSKLLFKVILVAGL 660
Query: 661 AAAWQYYFG 670
AAAWQYYFG
Sbjct: 661 AAAWQYYFG 669
BLAST of Lcy05g009630 vs. NCBI nr
Match:
XP_022953346.1 (GPI transamidase component GPI16 [Cucurbita moschata])
HSP 1 Score: 1239.9 bits (3207), Expect = 0.0e+00
Identity = 621/669 (92.83%), Postives = 642/669 (95.96%), Query Frame = 0
Query: 1 MASFLRLISLLCLPILFAVSAIGSVSEGAEEDFSEDLLLKPLPDRNVLAHFHFQSRAPSS 60
MASFLRLISLLCLPILFAV AIGSVSEG EEDFSEDLLLKPLPDR VLAHFHFQS APSS
Sbjct: 1 MASFLRLISLLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSGAPSS 60
Query: 61 RSNSYGRHHHVFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDTISSSNAKPSGVEL 120
RSNSYGRHHH+FPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFD+ISSSNAKPSGVEL
Sbjct: 61 RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDSISSSNAKPSGVEL 120
Query: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180
WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR
Sbjct: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180
Query: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSSEFDSNAVDSAIVL 240
EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLS+EFDSNAV+SAIVL
Sbjct: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVNSAIVL 240
Query: 241 DQTLTVVLQPYSHRGTLSYSTATQLQPSWSLSSIFGRRVTGRCALARSSNVYIQLDRGLM 300
DQTLTVVLQPYSHRG L YS A QLQPSWSLSSIFGR+V GRC LA SSNVYIQLD+GLM
Sbjct: 241 DQTLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLASSSNVYIQLDKGLM 300
Query: 301 AELQGMLGEQEILAITRTSSEGSRSNGAFELSANPDRVHMEISSRDDKHLSVLYMFIVEE 360
AELQGMLGEQE+LA+TR+SSEGS N AFELSANPDRVHME+SSRDDKHLSVLYMF V E
Sbjct: 301 AELQGMLGEQEMLAVTRSSSEGSIRNLAFELSANPDRVHMEMSSRDDKHLSVLYMFSVAE 360
Query: 361 KYDNSEPLDLRFTWKIPVFWSIPQAPLHVTRFLLGSGNERGAIALQLKSTKPSDRLMPDT 420
KYD SEPLDLRFTWKIPV WSIPQAPLHVTRF+LGSGNERGAIALQLKSTKPSD+L+PDT
Sbjct: 361 KYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPDT 420
Query: 421 VIKDSCSLLVRVFQVVPWYIKVYYHTLQVFIDNQPHTIKNVVEKMQVSPSKDKVSPGVME 480
VI DSCSLLV+VFQVVPWYIKVYYHTLQVFID+QPH+I NVVEKMQVSPS+DKVSPGV+E
Sbjct: 421 VITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLE 480
Query: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVISFPDFSTSTQFVENNSS 540
MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDA+QGLDIPSAVI+FPDF TSTQF ENNSS
Sbjct: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSS 540
Query: 541 KSPILSKLQGQSPILSYTEVLLVPLTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
KSPILSK QGQSPILSYTEVLLVP TTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG
Sbjct: 541 KSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
Query: 601 EEERFMKAKATKKFQVPQLVSKLLAKLRGRHWEPSESQAPSSASNSKSKLLFKVILVAGL 660
EEERFMK KA KKFQV QLVSKLLAKLRGRHWEPSESQAPSS+SNSKSKLLFKVILVAGL
Sbjct: 601 EEERFMKDKAKKKFQVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKSKLLFKVILVAGL 660
Query: 661 AAAWQYYFG 670
AAAWQYYFG
Sbjct: 661 AAAWQYYFG 669
BLAST of Lcy05g009630 vs. NCBI nr
Match:
XP_022991212.1 (GPI transamidase component GPI16 [Cucurbita maxima])
HSP 1 Score: 1234.2 bits (3192), Expect = 0.0e+00
Identity = 618/669 (92.38%), Postives = 640/669 (95.67%), Query Frame = 0
Query: 1 MASFLRLISLLCLPILFAVSAIGSVSEGAEEDFSEDLLLKPLPDRNVLAHFHFQSRAPSS 60
M SFLRLISLLCLPILFAV AIGSVSEG EEDFSEDLLLKPLPDR VLAHFHFQS APSS
Sbjct: 1 MVSFLRLISLLCLPILFAVPAIGSVSEGTEEDFSEDLLLKPLPDRKVLAHFHFQSVAPSS 60
Query: 61 RSNSYGRHHHVFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDTISSSNAKPSGVEL 120
RSNSYGRHHH+FPKAISQLVHKYRIKEMELSFTQGRW YDRWGGFD+ISSSNAKPSGVEL
Sbjct: 61 RSNSYGRHHHLFPKAISQLVHKYRIKEMELSFTQGRWHYDRWGGFDSISSSNAKPSGVEL 120
Query: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLPR 180
WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGF PASENMRYGTLPR
Sbjct: 121 WAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFRPASENMRYGTLPR 180
Query: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSSEFDSNAVDSAIVL 240
EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLS+EFDSNAVDSAIVL
Sbjct: 181 EAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSTEFDSNAVDSAIVL 240
Query: 241 DQTLTVVLQPYSHRGTLSYSTATQLQPSWSLSSIFGRRVTGRCALARSSNVYIQLDRGLM 300
DQTLTVVLQPYSHRG L YS A QLQPSWSLSSIFGR+V GRC LARSSNVYIQLD+GLM
Sbjct: 241 DQTLTVVLQPYSHRGALGYSNAAQLQPSWSLSSIFGRKVPGRCVLARSSNVYIQLDKGLM 300
Query: 301 AELQGMLGEQEILAITRTSSEGSRSNGAFELSANPDRVHMEISSRDDKHLSVLYMFIVEE 360
AELQGMLGEQE+LA+TR+SSEGS N AFELSANPDRVHME+SSR+DKHLSVLYMF V E
Sbjct: 301 AELQGMLGEQEMLAVTRSSSEGSTRNLAFELSANPDRVHMEMSSRNDKHLSVLYMFSVAE 360
Query: 361 KYDNSEPLDLRFTWKIPVFWSIPQAPLHVTRFLLGSGNERGAIALQLKSTKPSDRLMPDT 420
KYD SEPLDLRFTWKIPV WSIPQAPLHVTRF+LGSGNERGAIALQLKSTKPSD+L+P+T
Sbjct: 361 KYDESEPLDLRFTWKIPVVWSIPQAPLHVTRFVLGSGNERGAIALQLKSTKPSDQLIPNT 420
Query: 421 VIKDSCSLLVRVFQVVPWYIKVYYHTLQVFIDNQPHTIKNVVEKMQVSPSKDKVSPGVME 480
VI DSCSLLV+VFQVVPWYIKVYYHTLQVFID+QPH+I NVVEKMQVSPS+DKVSPGV+E
Sbjct: 421 VITDSCSLLVKVFQVVPWYIKVYYHTLQVFIDDQPHSITNVVEKMQVSPSEDKVSPGVLE 480
Query: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVISFPDFSTSTQFVENNSS 540
MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDA+QGLDIPSAVI+FPDF TSTQF ENNSS
Sbjct: 481 MLLKFPCGLKSAALTIEFDKGFLHIDEYPPDAHQGLDIPSAVITFPDFFTSTQFAENNSS 540
Query: 541 KSPILSKLQGQSPILSYTEVLLVPLTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
KSPILSK QGQSPILSYTEVLLVP TTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG
Sbjct: 541 KSPILSKWQGQSPILSYTEVLLVPFTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRVG 600
Query: 601 EEERFMKAKATKKFQVPQLVSKLLAKLRGRHWEPSESQAPSSASNSKSKLLFKVILVAGL 660
EEERFMK KA KKFQV QLVSKLLAKLRGRHWEPSESQAPSS+SNSKSKLLFKVILVAGL
Sbjct: 601 EEERFMKDKAKKKFQVSQLVSKLLAKLRGRHWEPSESQAPSSSSNSKSKLLFKVILVAGL 660
Query: 661 AAAWQYYFG 670
AAAWQYYFG
Sbjct: 661 AAAWQYYFG 669
BLAST of Lcy05g009630 vs. TAIR 10
Match:
AT3G07140.2 (GPI transamidase component Gpi16 subunit family protein )
HSP 1 Score: 784.6 bits (2025), Expect = 6.1e-227
Identity = 412/669 (61.58%), Postives = 500/669 (74.74%), Query Frame = 0
Query: 1 MASFLRLISLLCLPILFAVS-AIGSVSEGAEEDFSEDLLLKPLPDRNVLAHFHFQSRAPS 60
MAS LR + LL + F V+ A GS E+FSE LLLKPLPDR VLAHFHF++RAP
Sbjct: 1 MASLLRSLILLLIVQSFLVAIAFGSKE---VEEFSEALLLKPLPDRKVLAHFHFENRAPP 60
Query: 61 SRSNSYGRHHHVFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDTISSSNAKPSGVE 120
SNS+GRHHH+FPKAISQLV K+R+KEMELSFTQGRW ++ WGGFD +SS NAKP GVE
Sbjct: 61 --SNSHGRHHHLFPKAISQLVQKFRVKEMELSFTQGRWNHEHWGGFDPLSSMNAKPVGVE 120
Query: 121 LWAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLP 180
LWAVFDVP ++VD SWKNLTH+LSGLFCASINFLESSTSY+AP WGF P S+ +RYG+LP
Sbjct: 121 LWAVFDVPQSQVDTSWKNLTHALSGLFCASINFLESSTSYAAPTWGFGPNSDKLRYGSLP 180
Query: 181 REAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSSEFDSNAVDSAIV 240
REAVCTENLTPWLKLLPCRDK G+S LM+RPS+Y+GFYHSQRLHL + E + S IV
Sbjct: 181 REAVCTENLTPWLKLLPCRDKDGISALMNRPSVYRGFYHSQRLHLSTVESGQEGLGSGIV 240
Query: 241 LDQTLTVVLQPYSHRGTLSYSTATQLQPSWSLSSIFGRRVTGRCALARSSNVYIQLDRGL 300
L+QTLTVVLQP + S + +QPSWSLSS+FGR+V GRC LA+SSNVY+Q
Sbjct: 241 LEQTLTVVLQPE------TTSVESNMQPSWSLSSLFGRQVVGRCVLAKSSNVYLQ----- 300
Query: 301 MAELQGMLGEQEILAITRTSSEGSRSNGAFELSANPDRVHMEISSRDDKHLSVLYMFIVE 360
L+G+LG + T + N FELS P+RV E S L++F ++
Sbjct: 301 ---LEGLLGYESKNVDTEIEAHQLWKNAEFELSLKPERVIRE-------SCSFLFIFDID 360
Query: 361 EKYDNSEPLDLRFTWKIPVFWSIPQAPLHVTRFLLGSGNERGAIALQLKSTKPSDRLMPD 420
+ D SEP DL TWK P WS QAPLH +RFL+GSGNERGAIA+ LK+T+ ++L
Sbjct: 361 KSSD-SEPFDLGLTWKRPSKWSCQQAPLHSSRFLMGSGNERGAIAILLKATESQEKLSGR 420
Query: 421 TVIKDSCSLLVRVFQVVPWYIKVYYHTLQVFIDNQPHTIKNVVEKMQVSPSKDKVSPGVM 480
+ C++ +FQ+ PWYIKVYYHTLQ+F+D Q T V++K+ VSPS DKVS G+M
Sbjct: 421 DLTNGQCTIKANIFQIFPWYIKVYYHTLQIFVDQQQKTDSEVLKKINVSPSTDKVSSGMM 480
Query: 481 EMLLKFPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVISFPDFSTSTQFVENNS 540
EM+L+ PC +KS A++IE+DKGFLHIDEYPPDANQG DIPSA+ISFPD S F E
Sbjct: 481 EMMLELPCEVKSVAISIEYDKGFLHIDEYPPDANQGFDIPSALISFPDHHASLDFQE-EL 540
Query: 541 SKSPILSKLQGQSPILSYTEVLLVPLTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRV 600
S SP+LS L+ +S + SYTEVLLVPLTTPDFSMPYNVITITCT+ ALYFGSLLNVLRRR+
Sbjct: 541 SNSPLLSSLKEKSLVRSYTEVLLVPLTTPDFSMPYNVITITCTIFALYFGSLLNVLRRRI 600
Query: 601 GEEERFMKAKATKKFQVPQLVSKLLAKLRGRHWE-PSESQAPSSASNSKSKLLFKVILVA 660
GEEERF+K++ K + QL+S++ AK+RGR E PS S+A SS + SKL+ K+ILVA
Sbjct: 601 GEEERFLKSQGKKTGGLKQLLSRITAKIRGRPIEAPSSSEAESSVLS--SKLILKIILVA 639
Query: 661 GLAAAWQYY 668
G AAAWQY+
Sbjct: 661 GAAAAWQYF 639
BLAST of Lcy05g009630 vs. TAIR 10
Match:
AT3G07140.1 (GPI transamidase component Gpi16 subunit family protein )
HSP 1 Score: 783.9 bits (2023), Expect = 1.0e-226
Identity = 414/670 (61.79%), Postives = 502/670 (74.93%), Query Frame = 0
Query: 1 MASFLRLISLLCLPILFAVS-AIGSVSEGAEEDFSEDLLLKPLPDRNVLAHFHFQSRAPS 60
MAS LR + LL + F V+ A GS E+FSE LLLKPLPDR VLAHFHF++RAP
Sbjct: 1 MASLLRSLILLLIVQSFLVAIAFGSKE---VEEFSEALLLKPLPDRKVLAHFHFENRAPP 60
Query: 61 SRSNSYGRHHHVFPKAISQLVHKYRIKEMELSFTQGRWRYDRWGGFDTISSSNAKPSGVE 120
SNS+GRHHH+FPKAISQLV K+R+KEMELSFTQGRW ++ WGGFD +SS NAKP GVE
Sbjct: 61 --SNSHGRHHHLFPKAISQLVQKFRVKEMELSFTQGRWNHEHWGGFDPLSSMNAKPVGVE 120
Query: 121 LWAVFDVPPNEVDASWKNLTHSLSGLFCASINFLESSTSYSAPKWGFHPASENMRYGTLP 180
LWAVFDVP ++VD SWKNLTH+LSGLFCASINFLESSTSY+AP WGF P S+ +RYG+LP
Sbjct: 121 LWAVFDVPQSQVDTSWKNLTHALSGLFCASINFLESSTSYAAPTWGFGPNSDKLRYGSLP 180
Query: 181 REAVCTENLTPWLKLLPCRDKSGLSVLMDRPSIYKGFYHSQRLHLLSSEFDSNAVDSAIV 240
REAVCTENLTPWLKLLPCRDK G+S LM+RPS+Y+GFYHSQRLHL + E + S IV
Sbjct: 181 REAVCTENLTPWLKLLPCRDKDGISALMNRPSVYRGFYHSQRLHLSTVESGQEGLGSGIV 240
Query: 241 LDQTLTVVLQPYSHRGTLSYSTATQLQPSWSLSSIFGRRVTGRCALARSSNVYIQLDRGL 300
L+QTLTVVLQP + S + +QPSWSLSS+FGR+V GRC LA+SSNVY+Q
Sbjct: 241 LEQTLTVVLQPE------TTSVESNMQPSWSLSSLFGRQVVGRCVLAKSSNVYLQ----- 300
Query: 301 MAELQGMLGEQEILAITRTSSEGSRSNGAFELSANPDRVHMEISSRDDKHLSVLYMFIVE 360
L+G+LG + T + N FELS P+RV E S L++F ++
Sbjct: 301 ---LEGLLGYESKNVDTEIEAHQLWKNAEFELSLKPERVIRE-------SCSFLFIFDID 360
Query: 361 EKYDNSEPLDLRFTWKIPVFWSIPQAPLHVTRFLLGSGNERGAIALQLKSTKPSDRLMPD 420
+ D SEP DL TWK P WS QAPLH +RFL+GSGNERGAIA+ LK+T+ ++L
Sbjct: 361 KSSD-SEPFDLGLTWKRPSKWSCQQAPLHSSRFLMGSGNERGAIAILLKATESQEKLSGR 420
Query: 421 TVIKDSCSLLVRVFQVVPWYIKVYYHTLQVFIDNQPHTIKNVVEKMQVSPSKDKVSPGVM 480
+ C++ +FQ+ PWYIKVYYHTLQ+F+D Q T V++K+ VSPS DKVS G+M
Sbjct: 421 DLTNGQCTIKANIFQIFPWYIKVYYHTLQIFVDQQQKTDSEVLKKINVSPSTDKVSSGMM 480
Query: 481 EMLLKFPCGLKSAALTIEFDKGFLHIDEYPPDANQGLDIPSAVISFPDFSTSTQFVENNS 540
EM+L+ PC +KS A++IE+DKGFLHIDEYPPDANQG DIPSA+ISFPD S F E
Sbjct: 481 EMMLELPCEVKSVAISIEYDKGFLHIDEYPPDANQGFDIPSALISFPDHHASLDFQE-EL 540
Query: 541 SKSPILSKLQGQSPILSYTEVLLVPLTTPDFSMPYNVITITCTVLALYFGSLLNVLRRRV 600
S SP+LS L+ +S + SYTEVLLVPLTTPDFSMPYNVITITCT+ ALYFGSLLNVLRRR+
Sbjct: 541 SNSPLLSSLKEKSLVRSYTEVLLVPLTTPDFSMPYNVITITCTIFALYFGSLLNVLRRRI 600
Query: 601 GEEERFMKAKATKKF-QVPQLVSKLLAKLRGRHWE-PSESQAPSSASNSKSKLLFKVILV 660
GEEERF+K++A KK + QL+S++ AK+RGR E PS S+A SS + SKL+ K+ILV
Sbjct: 601 GEEERFLKSQAGKKTGGLKQLLSRITAKIRGRPIEAPSSSEAESSVLS--SKLILKIILV 640
Query: 661 AGLAAAWQYY 668
AG AAAWQY+
Sbjct: 661 AGAAAAWQYF 640
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q969N2 | 3.5e-78 | 33.16 | GPI transamidase component PIG-T OS=Homo sapiens OX=9606 GN=PIGT PE=1 SV=1 | [more] |
Q8BXQ2 | 6.7e-77 | 33.06 | GPI transamidase component PIG-T OS=Mus musculus OX=10090 GN=Pigt PE=1 SV=2 | [more] |
P38875 | 4.8e-51 | 28.86 | GPI transamidase component GPI16 OS=Saccharomyces cerevisiae (strain ATCC 204508... | [more] |
O94380 | 3.8e-48 | 28.55 | GPI transamidase component PIG-T homolog OS=Schizosaccharomyces pombe (strain 97... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1GMR1 | 0.0e+00 | 92.83 | GPI transamidase component GPI16 OS=Cucurbita moschata OX=3662 GN=LOC111455925 P... | [more] |
A0A6J1JL56 | 0.0e+00 | 92.38 | GPI transamidase component GPI16 OS=Cucurbita maxima OX=3661 GN=LOC111487934 PE=... | [more] |
A0A0A0K6R2 | 0.0e+00 | 91.33 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G340200 PE=4 SV=1 | [more] |
A0A1S3C6Z6 | 0.0e+00 | 90.88 | GPI transamidase component PIG-T OS=Cucumis melo OX=3656 GN=LOC103497563 PE=4 SV... | [more] |
A0A6J1DDU7 | 0.0e+00 | 90.75 | GPI transamidase component PIG-T OS=Momordica charantia OX=3673 GN=LOC111019616 ... | [more] |
Match Name | E-value | Identity | Description | |
KAG7014511.1 | 0.0e+00 | 93.27 | GPI transamidase component PIG-T, partial [Cucurbita argyrosperma subsp. argyros... | [more] |
KAG6575986.1 | 0.0e+00 | 93.12 | GPI transamidase component PIG-T, partial [Cucurbita argyrosperma subsp. sororia... | [more] |
XP_023548556.1 | 0.0e+00 | 92.97 | GPI transamidase component PIG-T isoform X1 [Cucurbita pepo subsp. pepo] >XP_023... | [more] |
XP_022953346.1 | 0.0e+00 | 92.83 | GPI transamidase component GPI16 [Cucurbita moschata] | [more] |
XP_022991212.1 | 0.0e+00 | 92.38 | GPI transamidase component GPI16 [Cucurbita maxima] | [more] |
Match Name | E-value | Identity | Description | |
AT3G07140.2 | 6.1e-227 | 61.58 | GPI transamidase component Gpi16 subunit family protein | [more] |
AT3G07140.1 | 1.0e-226 | 61.79 | GPI transamidase component Gpi16 subunit family protein | [more] |