Lcy05g005060 (gene) Sponge gourd (P93075) v1

Overview


Name	Lcy05g005060
Type	gene
Organism	Luffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
Description	Pectinesterase
Location	Chr05: 4658971 .. 4662500 (+)
RNA-Seq Expression	Lcy05g005060
Synteny	Lcy05g005060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGGTCCTCTAGTTCCTATAGTGGAATCTTTATAGTATTCTTGGAGAGCCATGTGATTGGTCTGCCAATTGGGTCCTAAAATTCAAGATCTCTCTCTTACACACATATATATATTGATAATCTTCCTGTTTTTTTTGGTATGTAGGCCAAAAAAACAGGAGGCACTATTCTCATGGCTACAAAGAATGGCTTCTTCAAAGTTATTCTACTTCTCCTTTTTGTATGTAATTTGTTTCTAGAATCTTGTTCACTGAATGCTGAGGAGGAAAGGGACTACAAGAACTGGTTGTCATGGAACCTTCAAGATTACAAGAAGAAAACCGGTTTGGAAGCCCGGTCGGCAGCCAAACTCTGTGGCGAGTCAAAGGGTGGGGGTGGCGTTGGTGGCGGAGTTTTTGATGATAAATTGAAAAAGGCTGAGACCAACAAAGTGAGGATAATTGTTAGTCAAGACGGGACGGGCGACTTCATGACCATTGGAGAAGCTCTCAACAGCATTCCTAAACCTAATAGTAAAAGAGTCATTTTGGACATCAATTCTGGAATTTACAGGTACATAGGTCTCATTTGATAACCATTTGGTTTTTGGTTTTTGAAAATTATGTTTGTTTTTCCTGGATTTTCTTTTGTTCTGAAAATGTTTGAATTCTTGTCTAAATTCTAAAAACAAAAACAAGTTTTTGAAACCTTTTTTAGTTTTGAAAACTGAACTTGGTTTTTGAAACGATATACCGTCTAATGGTGTTATAATGTGAACTGCATGCAGTGAAAAAATCAGCATTCCTAAAACTCTACCCTTCGTGACGTTTTTTGGAAACGCCAGTGATCCGCCGACCATCACGGGAAACGACACGGCGTCGGGGACGGGAGGAGACGGTATGCCATTGGGGACTTTGAAGAGTGCAACGGTGGCTGTAAATGCCAATTACTTTGTAGCTATCAATATAAAGTTTGAGGTAAAAGATTCTTTTTGCTAGCTGCTAGAAACATTCCAAAAGAACTCCTGATATTAAATTCACTATCATTTAGAGTATCACTCACGGGTTTCTCAAAGAGAAAACAACAAACAACACAAACTTCCACAATTCCAATACAAAGCAATTGGGGACATAAGCAAAATTCAAAACCTAGAAATCCAAAAATGAACAAAGAATATAATGTTTCTTTTTGGCCTTAATACAATCAAAACAACATCCTCTATTGATACGAGACCTTTTGTTACAAACAATAACATCATATACTAAAAAGAGCCCGTGAACGTAACTTTGCAGAACACGGCGACGCACGAGATCGGATCGGTCCGAGGACAAGGAGTGGCCTTGAGAATCTCAGGGACAAAGGCTGCATTTCACAACTGCAGCTTTTATGGGGATCAAGACACACTATATGATCACAAGGGTCTTCATTACTTCAACAACTGCTACATCCAAGGCTCAGTGGACTTCATATTTGGCTATGGGAGGTCATTCTATGAGGTGAAATCGATTCATTTTCACAATGAACTCACAAACCCCATTAAAAAGTTCTCACCTTTAATCTTCCATTGTTGTTGGAAATGGCTGCAGAAGTGTTATTTGAACTCCATAACCAAGAAAGTGGCTTCTGTGACTGCTCAAAAGCGATCAAAATCTTCAATGGCAAGTGGGTTTTCGTTTAAAGACAGTGTTGTGACAGGAAGTGGACATATCTATTTGGGAAGAGCTTGGGGAGATTATTCTAGAGTTGTTTTTTCCTACACTTTCATGGATAAGATTGTTCTTCCTCAGGGTTGGAATGATTGGGGCTCCCAAAAACGCCATTCGTAAGCTCCATATTTTGCTTTTTGTTATTGCATTTTTTTTCCTCACACTCATGATTTTTTAGTTTACATTTTGTTTTCTTGGGAGAAAATTAGTGTTATTGGTACATGTACTAGAAGAATAAGTCATGAGGGTAAAATTTAATAGAAGGGATATATGACTTAGGTTTCACAAACTCTAGGGTGTGTCTATAGAAACATTTTTTTAAAAGCATATGATGTGTGTTTTAATCTAATTAATTAGGCAAAGAGGCCAAAGCGAGCTTAGCTCAGCAGGTAATTGACATGTACCCTCGACCATGAGGTCGGAGGTTCGAATCCCCCACCCCAAATGTTGATGTACTCAAAAAAAAAAAAAAATTATTAGGCAAAGAGAAAGGGGTAATTTGTGTAATTATCCACATCTTAAAAGGCTATGGAAAAGGGTATGGCTTTGAAAACCAAAAAGAAAAGCTATATCTACTTTCTCTCAAGAGAAGGTTATGTTGGTTTGTAAAGATTAAAGTGCTTGAAATTGATTGAAAGGTTTTTTTCTATCCTTGGGAGCAAAATTACAGATTTGGTCTTTATGATTTGGGCATATATAGTGTTTCAGTTCCTTTATTATGGTTTTAAAATTTTCAATTTAGTCTCCGTGGTTTGATCTTAGTTTCAATTTGGTTCTTTATGATTTGATTAAATCTTGTCAAGTTGTCTCATAAATTTTGTTCTACCCACTTGAAATACTCAACTTTAAGCCTCAATACAGACAAACTAACATAGTTATCCAAATAGAAAACTACTACATATAAAAAAATAAAAATTGTCAATATGATAATGACATGGGTGGTTTGTGAAGTTTTATTCAACTATAGGGACCAAATTATTGAAACTTTTGGCTCAGGCTAGTTTGTGAACTAAATTATAAGGACCAAATTGAAACTTTAATTTTAAAACCACAAGGATTGAATTGAAACTTTTAAAACTAGAGAGACCTAATTTGTAACTAATTAGCCTTTTTTTCAAGAATAGAAAACCCCATATACTTTTTTTTGTCCAACACAACATGTGGGAGTGAGAGTACAAAACTCAGACACATTTTGGTCAAAGATATAGTGTCTAAACTAGTTGAACTATATATACTTAGGTTCAATTCACCTCAATATATATACTTTGTAAAAGGCTAAAGCTATATCATGCAATCATTCACGAAACAACTTTTAAAAGTCGAACCAACCACACATATATATTCTTACCAAATTTCTTCCTAAAAGTTACAAGAAAACTTATTGTGAAAAAACCACTCAAAATGTTGAGATTTTAAGAGCAAATGTTTGTATTTGCAGGACTGTGTATTATGGAGAGTACAAGTGCAGTGGACCAGGTGCAGACCTAACAGGAAGAGTGCAATGGGCACACAACTTAACAGATGAAGAGGCTCAACCTTTCATTGGAACTCACTATGTTGAGGCTGATACTTGGCTTCTCAGCCCTTATAACTCTTAAATATTTAATTTTATCAACCCTTTCTTCTTTTTTGTCCTAAATTTATGCTCTTTACATTACATAATCCTTTTGGATATATGTTTGTCCAAATGTTTTAAAAAAAATATTATGGATAATTTTGAACTAAATTATATAAAAATCTCTAGCTTATTGTGATGTGTTCTAGATGTATTCCATTTAATGAAGAGGAACCCATGTAGAAATGGATTCACTATAAAAACAATGTTGAATTGGTACCTTTTAACTT

mRNA sequence

TGGGTCCTCTAGTTCCTATAGTGGAATCTTTATAGTATTCTTGGAGAGCCATGTGATTGGTCTGCCAATTGGGTCCTAAAATTCAAGATCTCTCTCTTACACACATATATATATTGATAATCTTCCTGTTTTTTTTGGTATGTAGGCCAAAAAAACAGGAGGCACTATTCTCATGGCTACAAAGAATGGCTTCTTCAAAGTTATTCTACTTCTCCTTTTTGTATGTAATTTGTTTCTAGAATCTTGTTCACTGAATGCTGAGGAGGAAAGGGACTACAAGAACTGGTTGTCATGGAACCTTCAAGATTACAAGAAGAAAACCGGTTTGGAAGCCCGGTCGGCAGCCAAACTCTGTGGCGAGTCAAAGGGTGGGGGTGGCGTTGGTGGCGGAGTTTTTGATGATAAATTGAAAAAGGCTGAGACCAACAAAGTGAGGATAATTGTTAGTCAAGACGGGACGGGCGACTTCATGACCATTGGAGAAGCTCTCAACAGCATTCCTAAACCTAATAGTAAAAGAGTCATTTTGGACATCAATTCTGGAATTTACAGTGAAAAAATCAGCATTCCTAAAACTCTACCCTTCGTGACGTTTTTTGGAAACGCCAGTGATCCGCCGACCATCACGGGAAACGACACGGCGTCGGGGACGGGAGGAGACGGTATGCCATTGGGGACTTTGAAGAGTGCAACGGTGGCTGTAAATGCCAATTACTTTGTAGCTATCAATATAAAGTTTGAGAACACGGCGACGCACGAGATCGGATCGGTCCGAGGACAAGGAGTGGCCTTGAGAATCTCAGGGACAAAGGCTGCATTTCACAACTGCAGCTTTTATGGGGATCAAGACACACTATATGATCACAAGGGTCTTCATTACTTCAACAACTGCTACATCCAAGGCTCAGTGGACTTCATATTTGGCTATGGGAGGTCATTCTATGAGGTGAAATCGATTCATTTTCACAATGAACTCACAAACCCCATTAAAAAGTTCTCACCTTTAATCTTCCATTGTTGTTGGAAATGGCTGCAGAAGTGTTATTTGAACTCCATAACCAAGAAAGTGGCTTCTGTGACTGCTCAAAAGCGATCAAAATCTTCAATGGCAAGTGGGTTTTCGTTTAAAGACAGTGTTGTGACAGGAAGTGGACATATCTATTTGGGAAGAGCTTGGGGAGATTATTCTAGAGTTGTTTTTTCCTACACTTTCATGGATAAGATTGTTCTTCCTCAGGGTTGGAATGATTGGGGCTCCCAAAAACGCCATTCGACTGTGTATTATGGAGAGTACAAGTGCAGTGGACCAGGTGCAGACCTAACAGGAAGAGTGCAATGGGCACACAACTTAACAGATGAAGAGGCTCAACCTTTCATTGGAACTCACTATGTTGAGGCTGATACTTGGCTTCTCAGCCCTTATAACTCTTAAATATTTAATTTTATCAACCCTTTCTTCTTTTTTGTCCTAAATTTATGCTCTTTACATTACATAATCCTTTTGGATATATGTTTGTCCAAATGTTTTAAAAAAAATATTATGGATAATTTTGAACTAAATTATATAAAAATCTCTAGCTTATTGTGATGTGTTCTAGATGTATTCCATTTAATGAAGAGGAACCCATGTAGAAATGGATTCACTATAAAAACAATGTTGAATTGGTACCTTTTAACTT

Coding sequence (CDS)

ATGGCTACAAAGAATGGCTTCTTCAAAGTTATTCTACTTCTCCTTTTTGTATGTAATTTGTTTCTAGAATCTTGTTCACTGAATGCTGAGGAGGAAAGGGACTACAAGAACTGGTTGTCATGGAACCTTCAAGATTACAAGAAGAAAACCGGTTTGGAAGCCCGGTCGGCAGCCAAACTCTGTGGCGAGTCAAAGGGTGGGGGTGGCGTTGGTGGCGGAGTTTTTGATGATAAATTGAAAAAGGCTGAGACCAACAAAGTGAGGATAATTGTTAGTCAAGACGGGACGGGCGACTTCATGACCATTGGAGAAGCTCTCAACAGCATTCCTAAACCTAATAGTAAAAGAGTCATTTTGGACATCAATTCTGGAATTTACAGTGAAAAAATCAGCATTCCTAAAACTCTACCCTTCGTGACGTTTTTTGGAAACGCCAGTGATCCGCCGACCATCACGGGAAACGACACGGCGTCGGGGACGGGAGGAGACGGTATGCCATTGGGGACTTTGAAGAGTGCAACGGTGGCTGTAAATGCCAATTACTTTGTAGCTATCAATATAAAGTTTGAGAACACGGCGACGCACGAGATCGGATCGGTCCGAGGACAAGGAGTGGCCTTGAGAATCTCAGGGACAAAGGCTGCATTTCACAACTGCAGCTTTTATGGGGATCAAGACACACTATATGATCACAAGGGTCTTCATTACTTCAACAACTGCTACATCCAAGGCTCAGTGGACTTCATATTTGGCTATGGGAGGTCATTCTATGAGGTGAAATCGATTCATTTTCACAATGAACTCACAAACCCCATTAAAAAGTTCTCACCTTTAATCTTCCATTGTTGTTGGAAATGGCTGCAGAAGTGTTATTTGAACTCCATAACCAAGAAAGTGGCTTCTGTGACTGCTCAAAAGCGATCAAAATCTTCAATGGCAAGTGGGTTTTCGTTTAAAGACAGTGTTGTGACAGGAAGTGGACATATCTATTTGGGAAGAGCTTGGGGAGATTATTCTAGAGTTGTTTTTTCCTACACTTTCATGGATAAGATTGTTCTTCCTCAGGGTTGGAATGATTGGGGCTCCCAAAAACGCCATTCGACTGTGTATTATGGAGAGTACAAGTGCAGTGGACCAGGTGCAGACCTAACAGGAAGAGTGCAATGGGCACACAACTTAACAGATGAAGAGGCTCAACCTTTCATTGGAACTCACTATGTTGAGGCTGATACTTGGCTTCTCAGCCCTTATAACTCTTAA

Protein sequence

MATKNGFFKVILLLLFVCNLFLESCSLNAEEERDYKNWLSWNLQDYKKKTGLEARSAAKLCGESKGGGGVGGGVFDDKLKKAETNKVRIIVSQDGTGDFMTIGEALNSIPKPNSKRVILDINSGIYSEKISIPKTLPFVTFFGNASDPPTITGNDTASGTGGDGMPLGTLKSATVAVNANYFVAINIKFENTATHEIGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCYIQGSVDFIFGYGRSFYEVKSIHFHNELTNPIKKFSPLIFHCCWKWLQKCYLNSITKKVASVTAQKRSKSSMASGFSFKDSVVTGSGHIYLGRAWGDYSRVVFSYTFMDKIVLPQGWNDWGSQKRHSTVYYGEYKCSGPGADLTGRVQWAHNLTDEEAQPFIGTHYVEADTWLLSPYNS

Homology

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BLAST of Lcy05g005060 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 2.3e-75
Identity = 147/347 (42.36%), Postives = 201/347 (57.93%), Query Frame = 0


Query: 68  GGVGGGVFDDKLKKAETNKVRIIVSQDGTGDFMTIGEALNSIPKPNSKRVILDINSGIYS 127
           G +   VF     K   +    +  +   GDF  I +A++S+P  N  RV++ +++G+Y 
Sbjct: 65  GSLKHSVFKAAKNKLFPSYTLTVHKKSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYK 124

Query: 128 EKISIPKTLPFVTFFGNASDPPTITGNDTASGTGGDGMPLGTLKSATVAVNANYFVAINI 187
           EK+SIP    F+T  G  ++  T+   DTA      G P+GT  SA+ AVN+ +FVA NI
Sbjct: 125 EKVSIPPLKAFITIEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNI 184

Query: 188 KFENTATHEI-GSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCYIQGSV 247
            F NT    + G+V  Q VALR+S   AAF  C   G QDTLYDH G HY+ +CYI+GSV
Sbjct: 185 TFRNTTPVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSV 244

Query: 248 DFIFGYGRSFYEVKSIHFHNELTNPIKKFSPLIFHCCWKWLQKCYLNSITKKVASVTAQK 307
           DFIFG   S YE                               C++++I  K+ +VTAQ 
Sbjct: 245 DFIFGNALSLYE------------------------------GCHVHAIADKLGAVTAQG 304

Query: 308 RSKSSMASGFSFKDSVVTGSGHIYLGRAWGDYSRVVFSYTFMDKIVLPQGWNDWGSQKRH 367
           RS     +GFSF    VTG+G +YLGRAWG +SRVVF+YT+MD I+LP+GW +WG   R 
Sbjct: 305 RSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGDPSRE 364

Query: 368 STVYYGEYKCSGPGADLTGRVQWAHNLTDEEAQPFIGTHYVEADTWL 414
            TV+YG+YKC+G GA+  GRV WA  LTDEEA+PF+   +++   W+
Sbjct: 365 MTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWI 381

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BLAST of Lcy05g005060 vs. ExPASy Swiss-Prot
Match: Q9FM79 (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 1.4e-69
Identity = 153/412 (37.14%), Postives = 218/412 (52.91%), Query Frame = 0


Query: 7   FFKVILLLLFVCNLFLE-SCSLNAEEERDYKNWLSWNLQDYKKKTGLEARSAAKLCGESK 66
           F   +LLL F   L L+ SC+L      + KN++SW      +   +E   + K      
Sbjct: 6   FIPAVLLLCFGVMLCLKSSCALQIGNNNELKNYISWEDLRVVEDGRIERSFSIK------ 65

Query: 67  GGGGVGGGVFDDKLKKAETNKVR--IIVSQDGTGDFMTIGEALNSIPKPNSKRVILDINS 126
                   V  +    A    VR  I+V ++G GD +T+  A++ +P  NS+RV + I  
Sbjct: 66  ---ENSNWVTTNANANANATNVRRVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILP 125

Query: 127 GIYSEKISIPKTLPFVTFFGNAS--DPPTITGNDTASGTGGDGMPLGTLKSATVAVNANY 186
           GIY EK+ +PK+ P+++F GN S      I+ +D AS  G DG  LGT ++A+V++ +++
Sbjct: 126 GIYREKVIVPKSKPYISFIGNESYAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDF 185

Query: 187 FVAINIKFENTATHEIGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCY 246
           F A  I FENT   E G    Q VALRI G KA F+     G QDTL+D  G HYF  CY
Sbjct: 186 FCATAITFENTVVAEAGEQGRQAVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCY 245

Query: 247 IQGSVDFIFGYGRSFYEVKSIHFHNELTNPIKKFSPLIFHCCWKWLQKCYLNSITKKVAS 306
           IQG+VDFIFG  +S Y                              Q C ++S  K+  +
Sbjct: 246 IQGNVDFIFGNAKSLY------------------------------QDCDIHSTAKRYGA 305

Query: 307 VTAQKRSKSSMASGFSFKDSVVTGSGHIYLGRAWGDYSRVVFSYTFMDKIVLPQGWNDWG 366
           + A  R   +  +GFSF +  ++G+G IYLGRAWG+YSR V+S  F+  I+ P GW+DW 
Sbjct: 306 IAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPVGWSDWK 365

Query: 367 SQKRHSTVYYGEYKCSGPGADLTGRVQWAHNLTDEEAQPFIGTHYVEADTWL 414
             +R   V +GEY C G GA+  GRV W+  LT +E +PF+G  ++  D WL
Sbjct: 366 HPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWL 378

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BLAST of Lcy05g005060 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 4.8e-65
Identity = 136/327 (41.59%), Postives = 180/327 (55.05%), Query Frame = 0


Query: 89  IIVSQDGTGDFMTIGEALNSIPKPNSKRVILDINSGIYSEKISIPKTLPFVTFFGNASDP 148
           I VS +G   F ++ +A++SIPK N+K + + I  G Y EK+ +P T P++TF G   D 
Sbjct: 61  ITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRDV 120

Query: 149 PTITGNDTASGTGGDGMPLGTLKSATVAVNANYFVAINIKFENTATHEIGSVRG-QGVAL 208
             I  +D AS  G +G  L T ++A+V V ANYF A NI F NTA   +  ++G Q VA 
Sbjct: 121 TAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVAF 180

Query: 209 RISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCYIQGSVDFIFGYGRSFYEVKSIHFHNE 268
           RISG KA F  C FYG QDTL D  G HYF  CYI+GS+DFIFG GRS Y          
Sbjct: 181 RISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMY---------- 240

Query: 269 LTNPIKKFSPLIFHCCWKWLQKCYLNSITKKVASVTAQKRSKSSMASGFSFKDSVVTGSG 328
                               + C L+SI  +  S+ A  R+     +GF+F    VTG+G
Sbjct: 241 --------------------KDCELHSIASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTG 300

Query: 329 HIYLGRAWGDYSRVVFSYTFMDKIVLPQGWNDWGSQKRHS-TVYYGEYKCSGPGADLTGR 388
            +Y+GRA G YSR+V++YT+ D +V   GW+DW  +   S T ++G Y C GPGA  T  
Sbjct: 301 PLYVGRAMGQYSRIVYAYTYFDALVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRG 357

Query: 389 VQWAHNLTDEEAQPFIGTHYVEADTWL 414
           V WA  L  E A PFI   +V    W+
Sbjct: 361 VSWARALDYESAHPFIAKSFVNGRHWI 357

[Close]

BLAST of Lcy05g005060 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 1.4e-61
Identity = 133/342 (38.89%), Postives = 177/342 (51.75%), Query Frame = 0


Query: 78  KLKKAETNKVRIIVSQDGTGDFMTIGEALNSIPKPNSKRVILDINSGIYSEKISIPKTLP 137
           +L+      + + V   G G+F  +  A++ +P  +S + ++ +NSG Y EK+++ +   
Sbjct: 80  RLRHQYKTSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKT 139

Query: 138 FVTFFGNASDPPTITGNDTASGTGGDGMPLGTLKSATVAVNANYFVAINIKFENTATH-E 197
            +   G      +I  NDTA   G       T  S +  V A  F A NI F+N A   +
Sbjct: 140 NLVIQGRGYQNTSIEWNDTAKSAG------NTADSFSFVVFAANFTAYNISFKNNAPEPD 199

Query: 198 IGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCYIQGSVDFIFGYGRSF 257
            G    Q VALRI G +AAF+ C FYG QDTL D KG H+F  C+IQGS+DFIFG GRS 
Sbjct: 200 PGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSL 259

Query: 258 YEVKSIHFHNELTNPIKKFSPLIFHCCWKWLQKCYLNSITK-----KVASVTAQKRSKSS 317
           Y                              Q C +NSI K        S+TAQ R    
Sbjct: 260 Y------------------------------QDCTINSIAKGNTSGVTGSITAQGRQSED 319

Query: 318 MASGFSFKDSVVTGSGHIYLGRAWGDYSRVVFSYTFMDKIVLPQGWNDWGSQKRHSTVYY 377
             SGFSF +  + GSG I LGRAWG Y+ VVFS T+M  I+ P+GWN+WG   +  TV +
Sbjct: 320 EQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITPEGWNNWGDSTKEKTVTF 379

Query: 378 GEYKCSGPGADLTGRVQWAHNLTDEEAQPFIGTHYVEADTWL 414
           GE+KC GPGAD   RV +   LTD EA  FI   +++ D WL
Sbjct: 380 GEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDGDEWL 385

[Close]

BLAST of Lcy05g005060 vs. ExPASy Swiss-Prot
Match: O23038 (Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2)

HSP 1 Score: 237.7 bits (605), Expect = 2.5e-61
Identity = 129/327 (39.45%), Postives = 184/327 (56.27%), Query Frame = 0


Query: 89  IIVSQDGTGDFMTIGEALNSIPKPNSKRVILDINSGIYSEKISIPKTLPFVTFFGNASDP 148
           + V ++G  +F T+  A++++   + +R ++ INSG+Y EK+ IPKT P +T  G   D 
Sbjct: 91  LCVDKNGCCNFTTVQSAVDAVGNFSQRRNVIWINSGMYYEKVVIPKTKPNITLQGQGFDI 150

Query: 149 PTITGNDTASGTGGDGMPLGTLKSATVAVNANYFVAINIKFENTA-THEIGSVRGQGVAL 208
             I  NDTA          GT   ATV V  + FVA NI F N A   + G V  Q VA+
Sbjct: 151 TAIAWNDTAYSAN------GTFYCATVQVFGSQFVAKNISFMNVAPIPKPGDVGAQAVAI 210

Query: 209 RISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCYIQGSVDFIFGYGRSFY-EVKSIHFHN 268
           RI+G ++AF  C F+G QDTL+D +G HYF +CYIQGS+DFIFG  +S Y + + I   N
Sbjct: 211 RIAGDESAFVGCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKSLYQDCRIISMAN 270

Query: 269 ELTNPIKKFSPLIFHCCWKWLQKCYLNSITKKVASVTAQKRSKSSMASGFSFKDSVVTGS 328
           +L+   K  +                        +VTA  RS     SGFSF +  + G+
Sbjct: 271 QLSPGSKAVN-----------------------GAVTANGRSSKDENSGFSFVNCTIGGT 330

Query: 329 GHIYLGRAWGDYSRVVFSYTFMDKIVLPQGWNDWGSQKRHSTVYYGEYKCSGPGADLTGR 388
           GH++LGRAW  YSRVVF  T M  ++ P+GWN++    R +T++YGEY CSGPGAD++ R
Sbjct: 331 GHVWLGRAWRPYSRVVFVSTTMTDVIAPEGWNNFNDPSRDATIFYGEYNCSGPGADMSKR 388

Query: 389 VQWAHNLTDEEAQPFIGTHYVEADTWL 414
             +   L + +    I T +++ D WL
Sbjct: 391 APYVQKLNETQVALLINTSFIDGDQWL 388

[Close]

BLAST of Lcy05g005060 vs. ExPASy TrEMBL
Match: A0A6J1CWP4 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111015484 PE=4 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 9.4e-181
Identity = 328/419 (78.28%), Postives = 353/419 (84.25%), Query Frame = 0


Query: 1   MATKNGFFKV-ILLLLFVCNLFLESCSLNAEEERDYKNWLSWNLQDYKKK-TGLEARSAA 60
           MAT++ FFKV +LLLLFV NLFL+S SL   EERDYKNW+SWNLQD+KKK  G+EA+S+A
Sbjct: 1   MATRSDFFKVTLLLLLFVFNLFLQSYSLKT-EERDYKNWVSWNLQDFKKKLAGMEAQSSA 60

Query: 61  KLCGESKGGGGVGGGVFDDKLKKAETNKVRIIVSQDGTGDFMTIGEALNSIPKPNSKRVI 120
              G   G GG G  V DDKL+KAE NKVRIIVSQD TGDFM+IGEAL+SIPKPN+KRVI
Sbjct: 61  AKLGRFGGIGGSGAVVLDDKLRKAEMNKVRIIVSQDATGDFMSIGEALHSIPKPNNKRVI 120

Query: 121 LDINSGIYSEKISIPKTLPFVTFFGNASDPPTITGNDTASGTGGDGMPLGTLKSATVAVN 180
           L IN G+YSEKI+IP+TLPF+TF GNAS+PPTI GNDTAS TG DG+PLGTLKSATV+V+
Sbjct: 121 LVINPGVYSEKINIPRTLPFITFLGNASNPPTIIGNDTASVTGDDGIPLGTLKSATVSVD 180

Query: 181 ANYFVAINIKFENTATHEIGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFN 240
           ANYFVAINIKFENTATHEIGSV+GQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFN
Sbjct: 181 ANYFVAINIKFENTATHEIGSVKGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFN 240

Query: 241 NCYIQGSVDFIFGYGRSFYEVKSIHFHNELTNPIKKFSPLIFHCCWKWLQKCYLNSITKK 300
           +CYIQGSVDFIFGYGRSFYE                              KC LNSI KK
Sbjct: 241 SCYIQGSVDFIFGYGRSFYE------------------------------KCLLNSIAKK 300

Query: 301 VASVTAQKRSKSSMASGFSFKDSVVTGSGHIYLGRAWGDYSRVVFSYTFMDKIVLPQGWN 360
           VASVTAQKRSKSSMASGFSFKDSVVTGSG IYLGRAWGDYSRVVFSYT+MDKIVLPQGWN
Sbjct: 301 VASVTAQKRSKSSMASGFSFKDSVVTGSGQIYLGRAWGDYSRVVFSYTYMDKIVLPQGWN 360

Query: 361 DWGSQKRHSTVYYGEYKCSGPGADLTGRVQWAHNLTDEEAQPFIGTHYVEADTWLLSPY 418
           DW S KRHSTVYYGEYKCSGPGA+ TGRV WAH LTDEEAQPFIGTHYVEADTWLLSPY
Sbjct: 361 DWSSPKRHSTVYYGEYKCSGPGANSTGRVPWAHKLTDEEAQPFIGTHYVEADTWLLSPY 388

[Close]

BLAST of Lcy05g005060 vs. ExPASy TrEMBL
Match: A0A1S3CF26 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103500003 PE=4 SV=1)

HSP 1 Score: 640.2 bits (1650), Expect = 6.1e-180
Identity = 328/414 (79.23%), Postives = 344/414 (83.09%), Query Frame = 0


Query: 8   FKVILLLLFVCNLFLESCSLNAEEER-DYKNWLSWNLQDYKKKTGLEARSAAKLCGESKG 67
           F ++LLLLFVCNLFL+S SLN EEER DYKNWLSWNLQ+YKKK GL  RS AKL     G
Sbjct: 6   FILLLLLLFVCNLFLQSYSLNIEEEREDYKNWLSWNLQNYKKKAGLVNRSTAKL-----G 65

Query: 68  GGGVGGGVFDDKLKKAETNKVRIIVSQDGTGDFMTIGEALNSIPKPNSKRVILDINSGIY 127
               GGGV DDKLKKAE NKVRIIVSQDGTGDF T+GEA++SIPKPNSKRVIL IN G+Y
Sbjct: 66  RSYKGGGVLDDKLKKAEMNKVRIIVSQDGTGDFKTVGEAISSIPKPNSKRVILVINPGVY 125

Query: 128 SEKISIPKTLPFVTFFGNAS-DPPTITGNDTASGTGGDGMPLGTLKSATVAVNANYFVAI 187
           SEKI+IPK+LPFVTF GN S D PTITGNDTAS TG DG PLGTLKSATVAV+ANYFVAI
Sbjct: 126 SEKITIPKSLPFVTFLGNGSDDQPTITGNDTASVTGADGKPLGTLKSATVAVDANYFVAI 185

Query: 188 NIKFENTATHEIGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCYIQGS 247
           N+KFEN A HEIGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCYIQGS
Sbjct: 186 NMKFENRAMHEIGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCYIQGS 245

Query: 248 VDFIFGYGRSFYEVKSIHFHNELTNPIKKFSPLIFHCCWKWLQKCYLNSITKKVASVTAQ 307
           VDFIFGYGRSFYE                              KCYL SITKKVAS+TAQ
Sbjct: 246 VDFIFGYGRSFYE------------------------------KCYLKSITKKVASMTAQ 305

Query: 308 KRSKSSMASGFSFKDSVVTGSGHIYLGRAWGDYSRVVFSYTFMDKIVLPQGWNDWGSQKR 367
           K  K SM SGFSFKDSVVTGSG IYLGRAWGDYSRVVFSYTFMD IVLPQGWNDWGSQKR
Sbjct: 306 KGLKGSMESGFSFKDSVVTGSGQIYLGRAWGDYSRVVFSYTFMDNIVLPQGWNDWGSQKR 365

Query: 368 HSTVYYGEYKCSGPGADLTGRVQWAHNLTDEEAQPFIGTHYVEADTWLLSPYNS 420
           H TVYYGEYKCSGPGADL GRVQWAHNLTDEEAQPFIGTHYV+AD+WLLSPY+S
Sbjct: 366 HLTVYYGEYKCSGPGADLKGRVQWAHNLTDEEAQPFIGTHYVDADSWLLSPYSS 384

[Close]

BLAST of Lcy05g005060 vs. ExPASy TrEMBL
Match: A0A0A0KBA2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G414390 PE=4 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 2.0e-178
Identity = 327/417 (78.42%), Postives = 339/417 (81.29%), Query Frame = 0


Query: 5   NGFFKVILLLLFVCNLFLESCSLNAEEER-DYKNWLSWNLQDYKKKTGLEARSAAKLCGE 64
           N F  ++LLLLFVCNLFL+S SLN EE R DYKNWLSWNLQ+YKKK  L  RS  KL   
Sbjct: 2   NDFKFILLLLLFVCNLFLQSYSLNVEERREDYKNWLSWNLQNYKKKASLVDRSTVKL--- 61

Query: 65  SKGGGGVGGGVFDDKLKKAETNKVRIIVSQDGTGDFMTIGEALNSIPKPNSKRVILDINS 124
             G     GGV DDKLKKAE NKVRIIVSQDGTGDF T+GEALNSIPKPNSKRVIL IN 
Sbjct: 62  --GRSYNSGGVLDDKLKKAEMNKVRIIVSQDGTGDFRTVGEALNSIPKPNSKRVILVINP 121

Query: 125 GIYSEKISIPKTLPFVTFFGNA-SDPPTITGNDTASGTGGDGMPLGTLKSATVAVNANYF 184
           G+YSEKI IPK+LPFVTF GN   D PTITGNDTAS TG DG PLGTLKSATVAVNANYF
Sbjct: 122 GVYSEKIIIPKSLPFVTFLGNVIDDQPTITGNDTASMTGEDGKPLGTLKSATVAVNANYF 181

Query: 185 VAINIKFENTATHEIGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCYI 244
           VAIN+KFEN A HEIGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCYI
Sbjct: 182 VAINMKFENRAMHEIGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCYI 241

Query: 245 QGSVDFIFGYGRSFYEVKSIHFHNELTNPIKKFSPLIFHCCWKWLQKCYLNSITKKVASV 304
           QGSVDFIFGYGRSFYE                              KCYL SITKKVAS+
Sbjct: 242 QGSVDFIFGYGRSFYE------------------------------KCYLKSITKKVASM 301

Query: 305 TAQKRSKSSMASGFSFKDSVVTGSGHIYLGRAWGDYSRVVFSYTFMDKIVLPQGWNDWGS 364
           TAQK  K SM SGFSFKDSVVTGSG IYLGRAWGDYSRVVFSYTFMD IVLPQGWNDWGS
Sbjct: 302 TAQKGLKGSMESGFSFKDSVVTGSGQIYLGRAWGDYSRVVFSYTFMDNIVLPQGWNDWGS 361

Query: 365 QKRHSTVYYGEYKCSGPGADLTGRVQWAHNLTDEEAQPFIGTHYVEADTWLLSPYNS 420
           QKRH TVYYGEYKCSGPGADL GRVQWAHNLTDEEAQPFIGTHYV+AD+WLLSPY+S
Sbjct: 362 QKRHLTVYYGEYKCSGPGADLKGRVQWAHNLTDEEAQPFIGTHYVDADSWLLSPYSS 383

[Close]

BLAST of Lcy05g005060 vs. ExPASy TrEMBL
Match: A0A6J1GRP9 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456419 PE=4 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.6e-178
Identity = 321/421 (76.25%), Postives = 346/421 (82.19%), Query Frame = 0


Query: 1   MATKNGFFKVILLLLFVCNLFLESCSLNAEEER-DYKNWLSWNLQDYKKKTGLEARSAAK 60
           MA  N    ++LLLL  CNLF++S SL+A EER DYKNWLSWNLQ+YKKK      +  K
Sbjct: 1   MAAMNDLKVLLLLLLLGCNLFMQSYSLSAVEEREDYKNWLSWNLQNYKKK------AVRK 60

Query: 61  LCGESKGGGGVGGGVFDDKLKKAETNKVRIIVSQDGTGDFMTIGEALNSIPKPNSKRVIL 120
              +   G   GGGV DDKL+KAE+NK+RI+VSQDGTGDF TIGEALNSIPKPNSKR++L
Sbjct: 61  PGSQPNSGWFQGGGVLDDKLRKAESNKMRIVVSQDGTGDFKTIGEALNSIPKPNSKRIVL 120

Query: 121 DINSGIYSEKISIPKTLPFVTFFGNAS-DPPTITGNDTASGTGGDGMPLGTLKSATVAVN 180
            IN G+YSEKI+IPKTLPFVTF G+AS DPPTITGNDTA   GGDG+PLGTLKSATVA+N
Sbjct: 121 VINPGVYSEKINIPKTLPFVTFLGSASHDPPTITGNDTAVVIGGDGIPLGTLKSATVAIN 180

Query: 181 ANYFVAINIKFENTATHEIGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFN 240
            NYFVAINIKFENTA HE+GSVRGQGVALR+SGTKAAFHNCSFYGDQDTLYDHKGLHYFN
Sbjct: 181 GNYFVAINIKFENTAMHEVGSVRGQGVALRVSGTKAAFHNCSFYGDQDTLYDHKGLHYFN 240

Query: 241 NCYIQGSVDFIFGYGRSFYEVKSIHFHNELTNPIKKFSPLIFHCCWKWLQKCYLNSITKK 300
           NCYIQGSVDFIFGYGRSFYE                              KCYLNSI KK
Sbjct: 241 NCYIQGSVDFIFGYGRSFYE------------------------------KCYLNSIAKK 300

Query: 301 VASVTAQKRSKSSMASGFSFKDSVVTGSGHIYLGRAWGDYSRVVFSYTFMDKIVLPQGWN 360
           VASVTAQKRSKSSM SGFSFKD VVTGSG IYLGRAWGDYSRVVF+YTFMDKIVLPQGWN
Sbjct: 301 VASVTAQKRSKSSMGSGFSFKDCVVTGSGQIYLGRAWGDYSRVVFTYTFMDKIVLPQGWN 360

Query: 361 DWGSQKRHSTVYYGEYKCSGPGADLTGRVQWAHNLTDEEAQPFIGTHYVEADTWLLSPYN 420
           DWGS+KR STVYYGEYKCSGPGADL+GRV WAHNLTDEEAQPFIGTHYVEADTWLL+PYN
Sbjct: 361 DWGSRKRQSTVYYGEYKCSGPGADLSGRVGWAHNLTDEEAQPFIGTHYVEADTWLLAPYN 385

[Close]

BLAST of Lcy05g005060 vs. ExPASy TrEMBL
Match: A0A6J1JT48 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111488099 PE=4 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 1.7e-177
Identity = 322/421 (76.48%), Postives = 347/421 (82.42%), Query Frame = 0


Query: 1   MATKNGFFKVILLLLFVCNLFLESCSLNAEEER-DYKNWLSWNLQDYKKKTGLEARSAAK 60
           MA  N   K++LLLL  CNLF++S SL+  EER DYKNWLSWNLQ+YKKK      +  K
Sbjct: 1   MAAMND-LKLLLLLLLGCNLFMQSYSLSDVEEREDYKNWLSWNLQNYKKK------AVWK 60

Query: 61  LCGESKGGGGVGGGVFDDKLKKAETNKVRIIVSQDGTGDFMTIGEALNSIPKPNSKRVIL 120
              +   G   G GV DDKL+KAE+NKVRI++SQDGTGDF+TIGEALNSIPKPNSKR++L
Sbjct: 61  PGSQPNSGWFQGVGVLDDKLRKAESNKVRIVISQDGTGDFVTIGEALNSIPKPNSKRIVL 120

Query: 121 DINSGIYSEKISIPKTLPFVTFFGNAS-DPPTITGNDTASGTGGDGMPLGTLKSATVAVN 180
            IN G+YSEKISIPKTLPFVTF G+AS DPPTI GNDTA   GGDG+PLGTLKSATVA+N
Sbjct: 121 VINPGVYSEKISIPKTLPFVTFLGSASHDPPTIIGNDTAVVIGGDGIPLGTLKSATVAIN 180

Query: 181 ANYFVAINIKFENTATHEIGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFN 240
           ANYFVAINIKFENTA HE+GSVRGQGVALR+SGTKAAFHNCSFYGDQDTLYDHKGLHYFN
Sbjct: 181 ANYFVAINIKFENTAMHEVGSVRGQGVALRVSGTKAAFHNCSFYGDQDTLYDHKGLHYFN 240

Query: 241 NCYIQGSVDFIFGYGRSFYEVKSIHFHNELTNPIKKFSPLIFHCCWKWLQKCYLNSITKK 300
           NCYIQGSVDFIFGYGRSFYE                              KCYLNSI KK
Sbjct: 241 NCYIQGSVDFIFGYGRSFYE------------------------------KCYLNSIAKK 300

Query: 301 VASVTAQKRSKSSMASGFSFKDSVVTGSGHIYLGRAWGDYSRVVFSYTFMDKIVLPQGWN 360
           VASVTAQKRSKSSM SGFSFKD VVTGSG IYLGRAWGDYSRVVFSYTFMDKIVLPQGWN
Sbjct: 301 VASVTAQKRSKSSMGSGFSFKDCVVTGSGQIYLGRAWGDYSRVVFSYTFMDKIVLPQGWN 360

Query: 361 DWGSQKRHSTVYYGEYKCSGPGADLTGRVQWAHNLTDEEAQPFIGTHYVEADTWLLSPYN 420
           DWGS+KR STVYYGEYKCSGPGADL+GRV+WAHNLTDEEAQPFIGTHYVEADTWLL+PYN
Sbjct: 361 DWGSRKRQSTVYYGEYKCSGPGADLSGRVRWAHNLTDEEAQPFIGTHYVEADTWLLAPYN 384

[Close]

BLAST of Lcy05g005060 vs. NCBI nr
Match: XP_022146220.1 (probable pectinesterase 53 [Momordica charantia])

HSP 1 Score: 642.9 bits (1657), Expect = 1.9e-180
Identity = 328/419 (78.28%), Postives = 353/419 (84.25%), Query Frame = 0


Query: 1   MATKNGFFKV-ILLLLFVCNLFLESCSLNAEEERDYKNWLSWNLQDYKKK-TGLEARSAA 60
           MAT++ FFKV +LLLLFV NLFL+S SL   EERDYKNW+SWNLQD+KKK  G+EA+S+A
Sbjct: 1   MATRSDFFKVTLLLLLFVFNLFLQSYSLKT-EERDYKNWVSWNLQDFKKKLAGMEAQSSA 60

Query: 61  KLCGESKGGGGVGGGVFDDKLKKAETNKVRIIVSQDGTGDFMTIGEALNSIPKPNSKRVI 120
              G   G GG G  V DDKL+KAE NKVRIIVSQD TGDFM+IGEAL+SIPKPN+KRVI
Sbjct: 61  AKLGRFGGIGGSGAVVLDDKLRKAEMNKVRIIVSQDATGDFMSIGEALHSIPKPNNKRVI 120

Query: 121 LDINSGIYSEKISIPKTLPFVTFFGNASDPPTITGNDTASGTGGDGMPLGTLKSATVAVN 180
           L IN G+YSEKI+IP+TLPF+TF GNAS+PPTI GNDTAS TG DG+PLGTLKSATV+V+
Sbjct: 121 LVINPGVYSEKINIPRTLPFITFLGNASNPPTIIGNDTASVTGDDGIPLGTLKSATVSVD 180

Query: 181 ANYFVAINIKFENTATHEIGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFN 240
           ANYFVAINIKFENTATHEIGSV+GQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFN
Sbjct: 181 ANYFVAINIKFENTATHEIGSVKGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFN 240

Query: 241 NCYIQGSVDFIFGYGRSFYEVKSIHFHNELTNPIKKFSPLIFHCCWKWLQKCYLNSITKK 300
           +CYIQGSVDFIFGYGRSFYE                              KC LNSI KK
Sbjct: 241 SCYIQGSVDFIFGYGRSFYE------------------------------KCLLNSIAKK 300

Query: 301 VASVTAQKRSKSSMASGFSFKDSVVTGSGHIYLGRAWGDYSRVVFSYTFMDKIVLPQGWN 360
           VASVTAQKRSKSSMASGFSFKDSVVTGSG IYLGRAWGDYSRVVFSYT+MDKIVLPQGWN
Sbjct: 301 VASVTAQKRSKSSMASGFSFKDSVVTGSGQIYLGRAWGDYSRVVFSYTYMDKIVLPQGWN 360

Query: 361 DWGSQKRHSTVYYGEYKCSGPGADLTGRVQWAHNLTDEEAQPFIGTHYVEADTWLLSPY 418
           DW S KRHSTVYYGEYKCSGPGA+ TGRV WAH LTDEEAQPFIGTHYVEADTWLLSPY
Sbjct: 361 DWSSPKRHSTVYYGEYKCSGPGANSTGRVPWAHKLTDEEAQPFIGTHYVEADTWLLSPY 388

[Close]

BLAST of Lcy05g005060 vs. NCBI nr
Match: XP_008461405.1 (PREDICTED: probable pectinesterase 53 isoform X1 [Cucumis melo])

HSP 1 Score: 640.2 bits (1650), Expect = 1.3e-179
Identity = 328/414 (79.23%), Postives = 344/414 (83.09%), Query Frame = 0


Query: 8   FKVILLLLFVCNLFLESCSLNAEEER-DYKNWLSWNLQDYKKKTGLEARSAAKLCGESKG 67
           F ++LLLLFVCNLFL+S SLN EEER DYKNWLSWNLQ+YKKK GL  RS AKL     G
Sbjct: 6   FILLLLLLFVCNLFLQSYSLNIEEEREDYKNWLSWNLQNYKKKAGLVNRSTAKL-----G 65

Query: 68  GGGVGGGVFDDKLKKAETNKVRIIVSQDGTGDFMTIGEALNSIPKPNSKRVILDINSGIY 127
               GGGV DDKLKKAE NKVRIIVSQDGTGDF T+GEA++SIPKPNSKRVIL IN G+Y
Sbjct: 66  RSYKGGGVLDDKLKKAEMNKVRIIVSQDGTGDFKTVGEAISSIPKPNSKRVILVINPGVY 125

Query: 128 SEKISIPKTLPFVTFFGNAS-DPPTITGNDTASGTGGDGMPLGTLKSATVAVNANYFVAI 187
           SEKI+IPK+LPFVTF GN S D PTITGNDTAS TG DG PLGTLKSATVAV+ANYFVAI
Sbjct: 126 SEKITIPKSLPFVTFLGNGSDDQPTITGNDTASVTGADGKPLGTLKSATVAVDANYFVAI 185

Query: 188 NIKFENTATHEIGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCYIQGS 247
           N+KFEN A HEIGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCYIQGS
Sbjct: 186 NMKFENRAMHEIGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCYIQGS 245

Query: 248 VDFIFGYGRSFYEVKSIHFHNELTNPIKKFSPLIFHCCWKWLQKCYLNSITKKVASVTAQ 307
           VDFIFGYGRSFYE                              KCYL SITKKVAS+TAQ
Sbjct: 246 VDFIFGYGRSFYE------------------------------KCYLKSITKKVASMTAQ 305

Query: 308 KRSKSSMASGFSFKDSVVTGSGHIYLGRAWGDYSRVVFSYTFMDKIVLPQGWNDWGSQKR 367
           K  K SM SGFSFKDSVVTGSG IYLGRAWGDYSRVVFSYTFMD IVLPQGWNDWGSQKR
Sbjct: 306 KGLKGSMESGFSFKDSVVTGSGQIYLGRAWGDYSRVVFSYTFMDNIVLPQGWNDWGSQKR 365

Query: 368 HSTVYYGEYKCSGPGADLTGRVQWAHNLTDEEAQPFIGTHYVEADTWLLSPYNS 420
           H TVYYGEYKCSGPGADL GRVQWAHNLTDEEAQPFIGTHYV+AD+WLLSPY+S
Sbjct: 366 HLTVYYGEYKCSGPGADLKGRVQWAHNLTDEEAQPFIGTHYVDADSWLLSPYSS 384

[Close]

BLAST of Lcy05g005060 vs. NCBI nr
Match: XP_023547765.1 (probable pectinesterase 53 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 640.2 bits (1650), Expect = 1.3e-179
Identity = 326/421 (77.43%), Postives = 349/421 (82.90%), Query Frame = 0


Query: 1   MATKNGFFKVILLLLFVCNLFLESCSLNAEEER-DYKNWLSWNLQDYKKKTGLEARSAAK 60
           MA  N   KV+LLLL  CNLF++S SL+A EER DYKNWLSWNLQ+YKKK      +  K
Sbjct: 1   MAAMND-LKVLLLLLLGCNLFMQSYSLSAVEEREDYKNWLSWNLQNYKKK------AVWK 60

Query: 61  LCGESKGGGGVGGGVFDDKLKKAETNKVRIIVSQDGTGDFMTIGEALNSIPKPNSKRVIL 120
              +   G   GGGV DDKL+KAE+NKVRI+VSQDGTGDFMTIGEALNSIPKPNSKR++L
Sbjct: 61  PGSQPNSGWFQGGGVLDDKLRKAESNKVRIVVSQDGTGDFMTIGEALNSIPKPNSKRIVL 120

Query: 121 DINSGIYSEKISIPKTLPFVTFFGNAS-DPPTITGNDTASGTGGDGMPLGTLKSATVAVN 180
            IN G+YSEKI+IPKTLPFVTF G+AS DPPTITGNDTA   GGDG+PLGTLKSATVA+N
Sbjct: 121 VINPGVYSEKINIPKTLPFVTFLGSASHDPPTITGNDTAVVIGGDGIPLGTLKSATVAIN 180

Query: 181 ANYFVAINIKFENTATHEIGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFN 240
           ANYFVAINIKFENTA HE+GSVRGQGVA+R+SGTKAAFHNCSFYGDQDTLYDHKGLHYFN
Sbjct: 181 ANYFVAINIKFENTAMHEVGSVRGQGVAMRVSGTKAAFHNCSFYGDQDTLYDHKGLHYFN 240

Query: 241 NCYIQGSVDFIFGYGRSFYEVKSIHFHNELTNPIKKFSPLIFHCCWKWLQKCYLNSITKK 300
           NCYIQGSVDFIFGYGRSFYE                              KCYLNSI KK
Sbjct: 241 NCYIQGSVDFIFGYGRSFYE------------------------------KCYLNSIAKK 300

Query: 301 VASVTAQKRSKSSMASGFSFKDSVVTGSGHIYLGRAWGDYSRVVFSYTFMDKIVLPQGWN 360
           VASVTAQKRSKSSM SGFSFKD VVTGSG IYLGRAWGDYSRVVFSYTFMDKIVLPQGWN
Sbjct: 301 VASVTAQKRSKSSMGSGFSFKDCVVTGSGQIYLGRAWGDYSRVVFSYTFMDKIVLPQGWN 360

Query: 361 DWGSQKRHSTVYYGEYKCSGPGADLTGRVQWAHNLTDEEAQPFIGTHYVEADTWLLSPYN 420
           DWGS+KR STVYYGEYKCSGPGADL+GRV WAHNLTDEEAQPFIGTHYVEADTWLL+PYN
Sbjct: 361 DWGSRKRQSTVYYGEYKCSGPGADLSGRVGWAHNLTDEEAQPFIGTHYVEADTWLLAPYN 384

[Close]

BLAST of Lcy05g005060 vs. NCBI nr
Match: XP_004136146.1 (probable pectinesterase 53 isoform X1 [Cucumis sativus] >KGN45041.1 hypothetical protein Csa_015994 [Cucumis sativus])

HSP 1 Score: 635.2 bits (1637), Expect = 4.0e-178
Identity = 327/417 (78.42%), Postives = 339/417 (81.29%), Query Frame = 0


Query: 5   NGFFKVILLLLFVCNLFLESCSLNAEEER-DYKNWLSWNLQDYKKKTGLEARSAAKLCGE 64
           N F  ++LLLLFVCNLFL+S SLN EE R DYKNWLSWNLQ+YKKK  L  RS  KL   
Sbjct: 2   NDFKFILLLLLFVCNLFLQSYSLNVEERREDYKNWLSWNLQNYKKKASLVDRSTVKL--- 61

Query: 65  SKGGGGVGGGVFDDKLKKAETNKVRIIVSQDGTGDFMTIGEALNSIPKPNSKRVILDINS 124
             G     GGV DDKLKKAE NKVRIIVSQDGTGDF T+GEALNSIPKPNSKRVIL IN 
Sbjct: 62  --GRSYNSGGVLDDKLKKAEMNKVRIIVSQDGTGDFRTVGEALNSIPKPNSKRVILVINP 121

Query: 125 GIYSEKISIPKTLPFVTFFGNA-SDPPTITGNDTASGTGGDGMPLGTLKSATVAVNANYF 184
           G+YSEKI IPK+LPFVTF GN   D PTITGNDTAS TG DG PLGTLKSATVAVNANYF
Sbjct: 122 GVYSEKIIIPKSLPFVTFLGNVIDDQPTITGNDTASMTGEDGKPLGTLKSATVAVNANYF 181

Query: 185 VAINIKFENTATHEIGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCYI 244
           VAIN+KFEN A HEIGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCYI
Sbjct: 182 VAINMKFENRAMHEIGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCYI 241

Query: 245 QGSVDFIFGYGRSFYEVKSIHFHNELTNPIKKFSPLIFHCCWKWLQKCYLNSITKKVASV 304
           QGSVDFIFGYGRSFYE                              KCYL SITKKVAS+
Sbjct: 242 QGSVDFIFGYGRSFYE------------------------------KCYLKSITKKVASM 301

Query: 305 TAQKRSKSSMASGFSFKDSVVTGSGHIYLGRAWGDYSRVVFSYTFMDKIVLPQGWNDWGS 364
           TAQK  K SM SGFSFKDSVVTGSG IYLGRAWGDYSRVVFSYTFMD IVLPQGWNDWGS
Sbjct: 302 TAQKGLKGSMESGFSFKDSVVTGSGQIYLGRAWGDYSRVVFSYTFMDNIVLPQGWNDWGS 361

Query: 365 QKRHSTVYYGEYKCSGPGADLTGRVQWAHNLTDEEAQPFIGTHYVEADTWLLSPYNS 420
           QKRH TVYYGEYKCSGPGADL GRVQWAHNLTDEEAQPFIGTHYV+AD+WLLSPY+S
Sbjct: 362 QKRHLTVYYGEYKCSGPGADLKGRVQWAHNLTDEEAQPFIGTHYVDADSWLLSPYSS 383

[Close]

BLAST of Lcy05g005060 vs. NCBI nr
Match: XP_022954039.1 (probable pectinesterase 53 [Cucurbita moschata])

HSP 1 Score: 634.8 bits (1636), Expect = 5.3e-178
Identity = 321/421 (76.25%), Postives = 346/421 (82.19%), Query Frame = 0


Query: 1   MATKNGFFKVILLLLFVCNLFLESCSLNAEEER-DYKNWLSWNLQDYKKKTGLEARSAAK 60
           MA  N    ++LLLL  CNLF++S SL+A EER DYKNWLSWNLQ+YKKK      +  K
Sbjct: 1   MAAMNDLKVLLLLLLLGCNLFMQSYSLSAVEEREDYKNWLSWNLQNYKKK------AVRK 60

Query: 61  LCGESKGGGGVGGGVFDDKLKKAETNKVRIIVSQDGTGDFMTIGEALNSIPKPNSKRVIL 120
              +   G   GGGV DDKL+KAE+NK+RI+VSQDGTGDF TIGEALNSIPKPNSKR++L
Sbjct: 61  PGSQPNSGWFQGGGVLDDKLRKAESNKMRIVVSQDGTGDFKTIGEALNSIPKPNSKRIVL 120

Query: 121 DINSGIYSEKISIPKTLPFVTFFGNAS-DPPTITGNDTASGTGGDGMPLGTLKSATVAVN 180
            IN G+YSEKI+IPKTLPFVTF G+AS DPPTITGNDTA   GGDG+PLGTLKSATVA+N
Sbjct: 121 VINPGVYSEKINIPKTLPFVTFLGSASHDPPTITGNDTAVVIGGDGIPLGTLKSATVAIN 180

Query: 181 ANYFVAINIKFENTATHEIGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFN 240
            NYFVAINIKFENTA HE+GSVRGQGVALR+SGTKAAFHNCSFYGDQDTLYDHKGLHYFN
Sbjct: 181 GNYFVAINIKFENTAMHEVGSVRGQGVALRVSGTKAAFHNCSFYGDQDTLYDHKGLHYFN 240

Query: 241 NCYIQGSVDFIFGYGRSFYEVKSIHFHNELTNPIKKFSPLIFHCCWKWLQKCYLNSITKK 300
           NCYIQGSVDFIFGYGRSFYE                              KCYLNSI KK
Sbjct: 241 NCYIQGSVDFIFGYGRSFYE------------------------------KCYLNSIAKK 300

Query: 301 VASVTAQKRSKSSMASGFSFKDSVVTGSGHIYLGRAWGDYSRVVFSYTFMDKIVLPQGWN 360
           VASVTAQKRSKSSM SGFSFKD VVTGSG IYLGRAWGDYSRVVF+YTFMDKIVLPQGWN
Sbjct: 301 VASVTAQKRSKSSMGSGFSFKDCVVTGSGQIYLGRAWGDYSRVVFTYTFMDKIVLPQGWN 360

Query: 361 DWGSQKRHSTVYYGEYKCSGPGADLTGRVQWAHNLTDEEAQPFIGTHYVEADTWLLSPYN 420
           DWGS+KR STVYYGEYKCSGPGADL+GRV WAHNLTDEEAQPFIGTHYVEADTWLL+PYN
Sbjct: 361 DWGSRKRQSTVYYGEYKCSGPGADLSGRVGWAHNLTDEEAQPFIGTHYVEADTWLLAPYN 385

[Close]

BLAST of Lcy05g005060 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 284.3 bits (726), Expect = 1.6e-76
Identity = 147/347 (42.36%), Postives = 201/347 (57.93%), Query Frame = 0


Query: 68  GGVGGGVFDDKLKKAETNKVRIIVSQDGTGDFMTIGEALNSIPKPNSKRVILDINSGIYS 127
           G +   VF     K   +    +  +   GDF  I +A++S+P  N  RV++ +++G+Y 
Sbjct: 65  GSLKHSVFKAAKNKLFPSYTLTVHKKSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYK 124

Query: 128 EKISIPKTLPFVTFFGNASDPPTITGNDTASGTGGDGMPLGTLKSATVAVNANYFVAINI 187
           EK+SIP    F+T  G  ++  T+   DTA      G P+GT  SA+ AVN+ +FVA NI
Sbjct: 125 EKVSIPPLKAFITIEGEGAEKTTVEWGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNI 184

Query: 188 KFENTATHEI-GSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCYIQGSV 247
            F NT    + G+V  Q VALR+S   AAF  C   G QDTLYDH G HY+ +CYI+GSV
Sbjct: 185 TFRNTTPVPLPGAVGKQAVALRVSADNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSV 244

Query: 248 DFIFGYGRSFYEVKSIHFHNELTNPIKKFSPLIFHCCWKWLQKCYLNSITKKVASVTAQK 307
           DFIFG   S YE                               C++++I  K+ +VTAQ 
Sbjct: 245 DFIFGNALSLYE------------------------------GCHVHAIADKLGAVTAQG 304

Query: 308 RSKSSMASGFSFKDSVVTGSGHIYLGRAWGDYSRVVFSYTFMDKIVLPQGWNDWGSQKRH 367
           RS     +GFSF    VTG+G +YLGRAWG +SRVVF+YT+MD I+LP+GW +WG   R 
Sbjct: 305 RSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWGDPSRE 364

Query: 368 STVYYGEYKCSGPGADLTGRVQWAHNLTDEEAQPFIGTHYVEADTWL 414
            TV+YG+YKC+G GA+  GRV WA  LTDEEA+PF+   +++   W+
Sbjct: 365 MTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWI 381

[Close]

BLAST of Lcy05g005060 vs. TAIR 10
Match: AT5G55590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 265.0 bits (676), Expect = 1.0e-70
Identity = 153/412 (37.14%), Postives = 218/412 (52.91%), Query Frame = 0


Query: 7   FFKVILLLLFVCNLFLE-SCSLNAEEERDYKNWLSWNLQDYKKKTGLEARSAAKLCGESK 66
           F   +LLL F   L L+ SC+L      + KN++SW      +   +E   + K      
Sbjct: 6   FIPAVLLLCFGVMLCLKSSCALQIGNNNELKNYISWEDLRVVEDGRIERSFSIK------ 65

Query: 67  GGGGVGGGVFDDKLKKAETNKVR--IIVSQDGTGDFMTIGEALNSIPKPNSKRVILDINS 126
                   V  +    A    VR  I+V ++G GD +T+  A++ +P  NS+RV + I  
Sbjct: 66  ---ENSNWVTTNANANANATNVRRVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILP 125

Query: 127 GIYSEKISIPKTLPFVTFFGNAS--DPPTITGNDTASGTGGDGMPLGTLKSATVAVNANY 186
           GIY EK+ +PK+ P+++F GN S      I+ +D AS  G DG  LGT ++A+V++ +++
Sbjct: 126 GIYREKVIVPKSKPYISFIGNESYAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDF 185

Query: 187 FVAINIKFENTATHEIGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCY 246
           F A  I FENT   E G    Q VALRI G KA F+     G QDTL+D  G HYF  CY
Sbjct: 186 FCATAITFENTVVAEAGEQGRQAVALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCY 245

Query: 247 IQGSVDFIFGYGRSFYEVKSIHFHNELTNPIKKFSPLIFHCCWKWLQKCYLNSITKKVAS 306
           IQG+VDFIFG  +S Y                              Q C ++S  K+  +
Sbjct: 246 IQGNVDFIFGNAKSLY------------------------------QDCDIHSTAKRYGA 305

Query: 307 VTAQKRSKSSMASGFSFKDSVVTGSGHIYLGRAWGDYSRVVFSYTFMDKIVLPQGWNDWG 366
           + A  R   +  +GFSF +  ++G+G IYLGRAWG+YSR V+S  F+  I+ P GW+DW 
Sbjct: 306 IAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPVGWSDWK 365

Query: 367 SQKRHSTVYYGEYKCSGPGADLTGRVQWAHNLTDEEAQPFIGTHYVEADTWL 414
             +R   V +GEY C G GA+  GRV W+  LT +E +PF+G  ++  D WL
Sbjct: 366 HPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWL 378

[Close]

BLAST of Lcy05g005060 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 250.0 bits (637), Expect = 3.4e-66
Identity = 136/327 (41.59%), Postives = 180/327 (55.05%), Query Frame = 0


Query: 89  IIVSQDGTGDFMTIGEALNSIPKPNSKRVILDINSGIYSEKISIPKTLPFVTFFGNASDP 148
           I VS +G   F ++ +A++SIPK N+K + + I  G Y EK+ +P T P++TF G   D 
Sbjct: 61  ITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRDV 120

Query: 149 PTITGNDTASGTGGDGMPLGTLKSATVAVNANYFVAINIKFENTATHEIGSVRG-QGVAL 208
             I  +D AS  G +G  L T ++A+V V ANYF A NI F NTA   +  ++G Q VA 
Sbjct: 121 TAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVAF 180

Query: 209 RISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCYIQGSVDFIFGYGRSFYEVKSIHFHNE 268
           RISG KA F  C FYG QDTL D  G HYF  CYI+GS+DFIFG GRS Y          
Sbjct: 181 RISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMY---------- 240

Query: 269 LTNPIKKFSPLIFHCCWKWLQKCYLNSITKKVASVTAQKRSKSSMASGFSFKDSVVTGSG 328
                               + C L+SI  +  S+ A  R+     +GF+F    VTG+G
Sbjct: 241 --------------------KDCELHSIASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTG 300

Query: 329 HIYLGRAWGDYSRVVFSYTFMDKIVLPQGWNDWGSQKRHS-TVYYGEYKCSGPGADLTGR 388
            +Y+GRA G YSR+V++YT+ D +V   GW+DW  +   S T ++G Y C GPGA  T  
Sbjct: 301 PLYVGRAMGQYSRIVYAYTYFDALVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRG 357

Query: 389 VQWAHNLTDEEAQPFIGTHYVEADTWL 414
           V WA  L  E A PFI   +V    W+
Sbjct: 361 VSWARALDYESAHPFIAKSFVNGRHWI 357

[Close]

BLAST of Lcy05g005060 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 238.4 bits (607), Expect = 1.0e-62
Identity = 133/342 (38.89%), Postives = 177/342 (51.75%), Query Frame = 0


Query: 78  KLKKAETNKVRIIVSQDGTGDFMTIGEALNSIPKPNSKRVILDINSGIYSEKISIPKTLP 137
           +L+      + + V   G G+F  +  A++ +P  +S + ++ +NSG Y EK+++ +   
Sbjct: 80  RLRHQYKTSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKT 139

Query: 138 FVTFFGNASDPPTITGNDTASGTGGDGMPLGTLKSATVAVNANYFVAINIKFENTATH-E 197
            +   G      +I  NDTA   G       T  S +  V A  F A NI F+N A   +
Sbjct: 140 NLVIQGRGYQNTSIEWNDTAKSAG------NTADSFSFVVFAANFTAYNISFKNNAPEPD 199

Query: 198 IGSVRGQGVALRISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCYIQGSVDFIFGYGRSF 257
            G    Q VALRI G +AAF+ C FYG QDTL D KG H+F  C+IQGS+DFIFG GRS 
Sbjct: 200 PGEADAQAVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSL 259

Query: 258 YEVKSIHFHNELTNPIKKFSPLIFHCCWKWLQKCYLNSITK-----KVASVTAQKRSKSS 317
           Y                              Q C +NSI K        S+TAQ R    
Sbjct: 260 Y------------------------------QDCTINSIAKGNTSGVTGSITAQGRQSED 319

Query: 318 MASGFSFKDSVVTGSGHIYLGRAWGDYSRVVFSYTFMDKIVLPQGWNDWGSQKRHSTVYY 377
             SGFSF +  + GSG I LGRAWG Y+ VVFS T+M  I+ P+GWN+WG   +  TV +
Sbjct: 320 EQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITPEGWNNWGDSTKEKTVTF 379

Query: 378 GEYKCSGPGADLTGRVQWAHNLTDEEAQPFIGTHYVEADTWL 414
           GE+KC GPGAD   RV +   LTD EA  FI   +++ D WL
Sbjct: 380 GEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDGDEWL 385

[Close]

BLAST of Lcy05g005060 vs. TAIR 10
Match: AT1G05310.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 237.7 bits (605), Expect = 1.7e-62
Identity = 129/327 (39.45%), Postives = 184/327 (56.27%), Query Frame = 0


Query: 89  IIVSQDGTGDFMTIGEALNSIPKPNSKRVILDINSGIYSEKISIPKTLPFVTFFGNASDP 148
           + V ++G  +F T+  A++++   + +R ++ INSG+Y EK+ IPKT P +T  G   D 
Sbjct: 91  LCVDKNGCCNFTTVQSAVDAVGNFSQRRNVIWINSGMYYEKVVIPKTKPNITLQGQGFDI 150

Query: 149 PTITGNDTASGTGGDGMPLGTLKSATVAVNANYFVAINIKFENTA-THEIGSVRGQGVAL 208
             I  NDTA          GT   ATV V  + FVA NI F N A   + G V  Q VA+
Sbjct: 151 TAIAWNDTAYSAN------GTFYCATVQVFGSQFVAKNISFMNVAPIPKPGDVGAQAVAI 210

Query: 209 RISGTKAAFHNCSFYGDQDTLYDHKGLHYFNNCYIQGSVDFIFGYGRSFY-EVKSIHFHN 268
           RI+G ++AF  C F+G QDTL+D +G HYF +CYIQGS+DFIFG  +S Y + + I   N
Sbjct: 211 RIAGDESAFVGCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKSLYQDCRIISMAN 270

Query: 269 ELTNPIKKFSPLIFHCCWKWLQKCYLNSITKKVASVTAQKRSKSSMASGFSFKDSVVTGS 328
           +L+   K  +                        +VTA  RS     SGFSF +  + G+
Sbjct: 271 QLSPGSKAVN-----------------------GAVTANGRSSKDENSGFSFVNCTIGGT 330

Query: 329 GHIYLGRAWGDYSRVVFSYTFMDKIVLPQGWNDWGSQKRHSTVYYGEYKCSGPGADLTGR 388
           GH++LGRAW  YSRVVF  T M  ++ P+GWN++    R +T++YGEY CSGPGAD++ R
Sbjct: 331 GHVWLGRAWRPYSRVVFVSTTMTDVIAPEGWNNFNDPSRDATIFYGEYNCSGPGADMSKR 388

Query: 389 VQWAHNLTDEEAQPFIGTHYVEADTWL 414
             +   L + +    I T +++ D WL
Sbjct: 391 APYVQKLNETQVALLINTSFIDGDQWL 388

The following BLAST results are available for this feature:

BLAST of Lcy05g005060 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYZ3	2.3e-75	42.36	Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1	[more]
Q9FM79	1.4e-69	37.14	Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1	[more]
Q8LPF3	4.8e-65	41.59	Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1	[more]
Q9ZQA3	1.4e-61	38.89	Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1	[more]
O23038	2.5e-61	39.45	Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2	[more]

BLAST of Lcy05g005060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CWP4	9.4e-181	78.28	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111015484 PE=4 SV=1	[more]
A0A1S3CF26	6.1e-180	79.23	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103500003 PE=4 SV=1	[more]
A0A0A0KBA2	2.0e-178	78.42	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G414390 PE=4 SV=1	[more]
A0A6J1GRP9	2.6e-178	76.25	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111456419 PE=4 SV=1	[more]
A0A6J1JT48	1.7e-177	76.48	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111488099 PE=4 SV=1	[more]

BLAST of Lcy05g005060 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146220.1	1.9e-180	78.28	probable pectinesterase 53 [Momordica charantia]	[more]
XP_008461405.1	1.3e-179	79.23	PREDICTED: probable pectinesterase 53 isoform X1 [Cucumis melo]	[more]
XP_023547765.1	1.3e-179	77.43	probable pectinesterase 53 [Cucurbita pepo subsp. pepo]	[more]
XP_004136146.1	4.0e-178	78.42	probable pectinesterase 53 isoform X1 [Cucumis sativus] >KGN45041.1 hypothetical...	[more]
XP_022954039.1	5.3e-178	76.25	probable pectinesterase 53 [Cucurbita moschata]	[more]

BLAST of Lcy05g005060 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G19730.1	1.6e-76	42.36	Pectin lyase-like superfamily protein	[more]
AT5G55590.1	1.0e-70	37.14	Pectin lyase-like superfamily protein	[more]
AT5G47500.1	3.4e-66	41.59	Pectin lyase-like superfamily protein	[more]
AT2G36710.1	1.0e-62	38.89	Pectin lyase-like superfamily protein	[more]
AT1G05310.1	1.7e-62	39.45	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 83..418 e-value: 1.5E-97 score: 328.8
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 89..263 e-value: 6.1E-41 score: 140.1 coord: 287..409 e-value: 7.6E-25 score: 87.2
None	No IPR available	PANTHER	PTHR31321:SF81	PECTINESTERASE	coord: 16..414
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 16..414
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 242..251
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 86..413

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lcy05g005060.1	Lcy05g005060.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity