Lcy05g005020 (gene) Sponge gourd (P93075) v1

Overview
NameLcy05g005020
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionPectin lyase-like superfamily protein
LocationChr05: 4599836 .. 4602150 (+)
RNA-Seq ExpressionLcy05g005020
SyntenyLcy05g005020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAACATGGGAGGCTTATCTCCAATCCCAGTGGGATGATTTTCTACTTATAACATTCAGCCTTTTCTTCTCTTTTTCTTTTTCTCAAACAATCAATTGCAGTCTCCATTTTCTCTATTAAAGGTCAATGCCTACATGAATTTGGTCGGTTCCACTGCTAAAATAATCAATCCAAAAGTAATAATTTTGCAGATCAAGCTTTGATTCACCTTCCACTTTCACTTAAAGTTGGTTTCTTTCTCATTATATAACACAAAGATTCAGCCTTTCTTGTTTTGTGTGTTCATTCCCAAGTCAAAATATAGGCTCTCTCTTGGTTTCAGTACGAATCCAAGCTGTTAAACACAAGTTTTTGAGAGTTTGGAGCTCAAAAAAATGTCTAGTAATTTTGTGTTGTCAATTGCATTTTGGTTTTCCTTTGTTTTTTGCCTTCTTTCTTCTTCTCAAGCTAGATGGCACCATCACACCAAGCAAAAACACTCCCACCACCATAAACATAGCTACATTTCACACCCACCTTCCCCTTCTCCTTCTGATTCACCCGACGACGGAGATTACATTGGCGAAACGAATATGTTCGACGTTAGAGCTTTCGGTGCCATCGGCGATGGAGTTGTAGACGACACGGAGGCGTTCAAGATGGCTTGGGACACGGCGTGCCAGAGTGACGACTCTGCGGTCATGCTTGTTCCGTATGGCTTCTCGTTTATGATTCAATCAACAATCTTCACTGGCCCTTGTCATGGTCACCTCATGTTGCGGGTCGACGGCACGTTGATGCCACCGGATGGCCCCGACGCCTGGCCGAAGAACGCTAGTCGTCGCCAGTGGTTGGTCTTTTACAGAATTGATGGAATGTCTTTAGAAGGAGGAGGTGTGATTGATGGGAGAGGTCAAAAGTGGTGGGATCTTCCATGCAAACCACACAAGGTATTCAATCCTTCTTCCATGTTTTTATGCAAATGCCTGCAATTTCCATCTCTTCTACAAGTTCTAACACAAGATATTTTTGAAACTTTAGGGACTAAATGGAACCACGTTACCGGGACCGTGTGATAGTCCAATTGTAAGTTCATATACCTCAAAAAATGTCTGCTGATGTGATATACTTTTCTGTTAAGATATGTGAAGAAACTTAGGCATCCCATTCTAATGATTTTAGGCTATAAGGTTCTTCATGAGCTCCAATTTGACTGTCCAAGGTCTTAAAATCACAAACAGCCCTCAATTCCACTTCAGATTCGACGGTTGCAAGAATGTTCATATCGACTCGATTCACATAAAATCTCCTGGTCTTAGTCCCAACACAGACGGTATCCACATCGAGAACACAAACGGCGTCGAGATATATAATTCAGTCATTTTCAATGGTCAGTACCATGAACCAAGAAAGGGCTAATCACATTGTTAAGGGAGTGAATCATATAATACATTCTGATTATTTATGTTTATGTAGGGGATGATTGTGTATCAATTGGATCAGGTGCATATGATGTAGATATCAGAAACATCACATGTGGACCAAGTCATGGTATCAGGTATTGTGTTTATTAGTAATTTTAAAACATAACTACACTGTTGTTTGGTTGTCAATCAAAGTAAAACGTAACTCAACTGATTAAGATATGAAATAATCTTTTCTAACTTTTTCCTCTCTCTTTTGTCCAGCATCGGAAGTCTCGGTGTTCGCAACTCGAGAGCCTGCGTTACGAACATAACAGTTAGGGACTCAGTAATCAAAGTTTCAGACAATGGAGTTCGAATCAAGACATGGCAAGGTGGCTATGGAGCAGTAAGAGGACTGACATTTAGCAACATTCACATGGACAATGTTAGGAATCCCATCATTATAGATCAATACTACTGCCTAACCAAAGATTGCACGAACCAAACCTCAGCTGTCTCAATATCAGACATTCAATACACAAACATCAAAGGAACATACGACATTCGGAGCCCGCCTATGCACTTCGGCTGCAGCGACTCCGTCCCGTGTACCAATCTAACGCTGTCGGAGATCGAGCTCCTTCCTGCTCAAGGAGACATAGTGTTGGACCCTTTTTGTTGGAATGCCTATGGAGATCTTCAAACAATTACCATTCCACCTGTCTCATGCTTGCAAGAAGGGCTTCCTTCTTCTATTGATTTAAACTATGACAATGAGTACTGTTGATCATTTTCCTTACTATACACATTTATAACAACATATGTGATCGTCATATATATGTATATACAATAATTACGGATGTATTTGAAATATGCTTAGATTAAAACTTTTATATGATGTCACAATACTACCCTCCCTTAGGACGTATC

mRNA sequence

AGAACATGGGAGGCTTATCTCCAATCCCAGTGGGATGATTTTCTACTTATAACATTCAGCCTTTTCTTCTCTTTTTCTTTTTCTCAAACAATCAATTGCAGTCTCCATTTTCTCTATTAAAGGTCAATGCCTACATGAATTTGGTCGGTTCCACTGCTAAAATAATCAATCCAAAAGTAATAATTTTGCAGATCAAGCTTTGATTCACCTTCCACTTTCACTTAAAGTTGGTTTCTTTCTCATTATATAACACAAAGATTCAGCCTTTCTTGTTTTGTGTGTTCATTCCCAAGTCAAAATATAGGCTCTCTCTTGGTTTCAGTACGAATCCAAGCTGTTAAACACAAGTTTTTGAGAGTTTGGAGCTCAAAAAAATGTCTAGTAATTTTGTGTTGTCAATTGCATTTTGGTTTTCCTTTGTTTTTTGCCTTCTTTCTTCTTCTCAAGCTAGATGGCACCATCACACCAAGCAAAAACACTCCCACCACCATAAACATAGCTACATTTCACACCCACCTTCCCCTTCTCCTTCTGATTCACCCGACGACGGAGATTACATTGGCGAAACGAATATGTTCGACGTTAGAGCTTTCGGTGCCATCGGCGATGGAGTTGTAGACGACACGGAGGCGTTCAAGATGGCTTGGGACACGGCGTGCCAGAGTGACGACTCTGCGGTCATGCTTGTTCCGTATGGCTTCTCGTTTATGATTCAATCAACAATCTTCACTGGCCCTTGTCATGGTCACCTCATGTTGCGGGTCGACGGCACGTTGATGCCACCGGATGGCCCCGACGCCTGGCCGAAGAACGCTAGTCGTCGCCAGTGGTTGGTCTTTTACAGAATTGATGGAATGTCTTTAGAAGGAGGAGGTGTGATTGATGGGAGAGGTCAAAAGTGGTGGGATCTTCCATGCAAACCACACAAGGGACTAAATGGAACCACGTTACCGGGACCGTGTGATAGTCCAATTGCTATAAGGTTCTTCATGAGCTCCAATTTGACTGTCCAAGGTCTTAAAATCACAAACAGCCCTCAATTCCACTTCAGATTCGACGGTTGCAAGAATGTTCATATCGACTCGATTCACATAAAATCTCCTGGTCTTAGTCCCAACACAGACGGTATCCACATCGAGAACACAAACGGCGTCGAGATATATAATTCAGTCATTTTCAATGGGGATGATTGTGTATCAATTGGATCAGGTGCATATGATGTAGATATCAGAAACATCACATGTGGACCAAGTCATGGTATCAGCATCGGAAGTCTCGGTGTTCGCAACTCGAGAGCCTGCGTTACGAACATAACAGTTAGGGACTCAGTAATCAAAGTTTCAGACAATGGAGTTCGAATCAAGACATGGCAAGGTGGCTATGGAGCAGTAAGAGGACTGACATTTAGCAACATTCACATGGACAATGTTAGGAATCCCATCATTATAGATCAATACTACTGCCTAACCAAAGATTGCACGAACCAAACCTCAGCTGTCTCAATATCAGACATTCAATACACAAACATCAAAGGAACATACGACATTCGGAGCCCGCCTATGCACTTCGGCTGCAGCGACTCCGTCCCGTGTACCAATCTAACGCTGTCGGAGATCGAGCTCCTTCCTGCTCAAGGAGACATAGTGTTGGACCCTTTTTGTTGGAATGCCTATGGAGATCTTCAAACAATTACCATTCCACCTGTCTCATGCTTGCAAGAAGGGCTTCCTTCTTCTATTGATTTAAACTATGACAATGAGTACTGTTGATCATTTTCCTTACTATACACATTTATAACAACATATGTGATCGTCATATATATGTATATACAATAATTACGGATGTATTTGAAATATGCTTAGATTAAAACTTTTATATGATGTCACAATACTACCCTCCCTTAGGACGTATC

Coding sequence (CDS)

ATGTCTAGTAATTTTGTGTTGTCAATTGCATTTTGGTTTTCCTTTGTTTTTTGCCTTCTTTCTTCTTCTCAAGCTAGATGGCACCATCACACCAAGCAAAAACACTCCCACCACCATAAACATAGCTACATTTCACACCCACCTTCCCCTTCTCCTTCTGATTCACCCGACGACGGAGATTACATTGGCGAAACGAATATGTTCGACGTTAGAGCTTTCGGTGCCATCGGCGATGGAGTTGTAGACGACACGGAGGCGTTCAAGATGGCTTGGGACACGGCGTGCCAGAGTGACGACTCTGCGGTCATGCTTGTTCCGTATGGCTTCTCGTTTATGATTCAATCAACAATCTTCACTGGCCCTTGTCATGGTCACCTCATGTTGCGGGTCGACGGCACGTTGATGCCACCGGATGGCCCCGACGCCTGGCCGAAGAACGCTAGTCGTCGCCAGTGGTTGGTCTTTTACAGAATTGATGGAATGTCTTTAGAAGGAGGAGGTGTGATTGATGGGAGAGGTCAAAAGTGGTGGGATCTTCCATGCAAACCACACAAGGGACTAAATGGAACCACGTTACCGGGACCGTGTGATAGTCCAATTGCTATAAGGTTCTTCATGAGCTCCAATTTGACTGTCCAAGGTCTTAAAATCACAAACAGCCCTCAATTCCACTTCAGATTCGACGGTTGCAAGAATGTTCATATCGACTCGATTCACATAAAATCTCCTGGTCTTAGTCCCAACACAGACGGTATCCACATCGAGAACACAAACGGCGTCGAGATATATAATTCAGTCATTTTCAATGGGGATGATTGTGTATCAATTGGATCAGGTGCATATGATGTAGATATCAGAAACATCACATGTGGACCAAGTCATGGTATCAGCATCGGAAGTCTCGGTGTTCGCAACTCGAGAGCCTGCGTTACGAACATAACAGTTAGGGACTCAGTAATCAAAGTTTCAGACAATGGAGTTCGAATCAAGACATGGCAAGGTGGCTATGGAGCAGTAAGAGGACTGACATTTAGCAACATTCACATGGACAATGTTAGGAATCCCATCATTATAGATCAATACTACTGCCTAACCAAAGATTGCACGAACCAAACCTCAGCTGTCTCAATATCAGACATTCAATACACAAACATCAAAGGAACATACGACATTCGGAGCCCGCCTATGCACTTCGGCTGCAGCGACTCCGTCCCGTGTACCAATCTAACGCTGTCGGAGATCGAGCTCCTTCCTGCTCAAGGAGACATAGTGTTGGACCCTTTTTGTTGGAATGCCTATGGAGATCTTCAAACAATTACCATTCCACCTGTCTCATGCTTGCAAGAAGGGCTTCCTTCTTCTATTGATTTAAACTATGACAATGAGTACTGTTGA

Protein sequence

MSSNFVLSIAFWFSFVFCLLSSSQARWHHHTKQKHSHHHKHSYISHPPSPSPSDSPDDGDYIGETNMFDVRAFGAIGDGVVDDTEAFKMAWDTACQSDDSAVMLVPYGFSFMIQSTIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIFNGDDCVSIGSGAYDVDIRNITCGPSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFGCSDSVPCTNLTLSEIELLPAQGDIVLDPFCWNAYGDLQTITIPPVSCLQEGLPSSIDLNYDNEYC
Homology
BLAST of Lcy05g005020 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 3.7e-159
Identity = 261/455 (57.36%), Postives = 335/455 (73.63%), Query Frame = 0

Query: 23  SQARWHHHTKQKHSHHH--------------KHSYISHPPSPSPSDSPDDGDYIGETNMF 82
           ++AR+H+H K +H   H                  +   P PSP  +P D D      +F
Sbjct: 25  ARARYHYH-KGRHGVTHPLPPPPPPPLETANPPDQVPSDPYPSPDPAPGDSD---SGCVF 84

Query: 83  DVRAFGAIGDGVVDDTEAFKMAWDTACQSDDSAVMLVPYGFSFMIQSTIFTGPCHGHLML 142
           DV +FGA+GDG  DDT AF+ AW  AC + +S V+L P G  F I STIF+GPC   L+ 
Sbjct: 85  DVTSFGAVGDGSCDDTAAFQDAWKAAC-AVESGVVLAPEGGVFKITSTIFSGPCKPGLVF 144

Query: 143 RVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKGLN 202
           ++DG LMPPDGP+ WP+  ++ QWLVFYR+DG +  G G ++G GQKWWDLPCKPH+G +
Sbjct: 145 QLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWDLPCKPHRGPD 204

Query: 203 GTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLSPN 262
           G++  GPC SP  IRFFMS+N+ V+GL+I NSPQFH +FDGC+ V I+ I I SP LSPN
Sbjct: 205 GSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISSPKLSPN 264

Query: 263 TDGIHIENTNGVEIYNSVIFNGDDCVSIGSGAYDVDIRNITCGPSHGISIGSLGVRNSRA 322
           TDGIH+ NT  V IYNSV+ NGDDC+SIG+G  DVDI+ +TCGPSHGISIGSLGV NS+A
Sbjct: 265 TDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGISIGSLGVHNSQA 324

Query: 323 CVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYYCLTKDC 382
           CV+NITVR++VI+ SDNG+R+KTWQGG G+V  L F NI M+NV N II+DQYYC +KDC
Sbjct: 325 CVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNCIIVDQYYCQSKDC 384

Query: 383 TNQTSAVSISDIQYTNIKGTYDIRSPPMHFGCSDSVPCTNLTLSEIELLPAQGDIVLDPF 442
            N+TSAV + D++Y NIKGTYD+RSPP+HF CSD+V CTN+T+SE+ELLP +G++V DPF
Sbjct: 385 RNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLPEEGELVDDPF 444

Query: 443 CWNAYGDLQTITIPPVSCLQEGLPSSIDLNYDNEY 464
           CWNAYG  +T+TIPP+ CL +G P  ++  YD+ Y
Sbjct: 445 CWNAYGKQETLTIPPIDCLLDGSP-VVEEAYDSNY 473

BLAST of Lcy05g005020 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 3.5e-93
Identity = 171/401 (42.64%), Postives = 236/401 (58.85%), Query Frame = 0

Query: 67  MFDVRAFGAIGDGVVDDTEAFKMAWDTACQSDDSAVMLVPYGFSFMIQSTIFTGPCHGHL 126
           +  V  FGA G+GV DDT+AF  AW TAC S     +LVP  ++ +++    +GPC   L
Sbjct: 52  LLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCKARL 111

Query: 127 MLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKG 186
            L++ GT++ P+ PD W +  +RR+WL F+ +  +++EGGG ++G GQ+WW+  CK H  
Sbjct: 112 TLQISGTIIAPNDPDVW-EGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCK-HNH 171

Query: 187 LNGTTLPGPC-DSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGL 246
            N      PC  +P A+ F    N+ V+ L + +S Q H     C+ V I  + + +P  
Sbjct: 172 SN------PCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPAT 231

Query: 247 SPNTDGIHIENTNGVEIYNSVIFNGDDCVSIGSGAYDVDIRNITCGPSHGISIGSLGVRN 306
           SPNTDGIHI  + G+ I N+ +  GDDC+SI   +  + I NI CGP HGISIGSLG   
Sbjct: 232 SPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSK 291

Query: 307 SRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYYC-L 366
           S   V +ITV  ++I  + NGVRIKTWQGG G V  + F NI M+NV NPIIIDQYYC  
Sbjct: 292 SWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDS 351

Query: 367 TKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFGCSDSVPCTNLTLSEIELLPAQGDIV 426
            K C NQTSA+SI +I + +++GT       +   CSDS PC N+ L +I+L P+ GD  
Sbjct: 352 RKPCANQTSAISIENISFVHVRGT-SASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGF 411

Query: 427 LDPFCWNAYGDLQTITIPP------VSCLQEGLPSSIDLNY 460
            + FCW AYG       PP       S L++ + S I   Y
Sbjct: 412 TESFCWEAYGSSSGQVYPPPCLSDDTSFLEQSVQSGITSAY 443

BLAST of Lcy05g005020 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 6.7e-84
Identity = 158/359 (44.01%), Postives = 216/359 (60.17%), Query Frame = 0

Query: 66  NMFDVRAFGAIGDGVVDDTEAFKMAWDTACQSDDSAVMLVPYGFSFMIQSTIFTGPCHGH 125
           N+F+V  +GA+GDG  D TEAF  AW  AC+   SA++LVP    F++ +  F GPC  H
Sbjct: 57  NIFNVEKYGAVGDGKHDCTEAFSTAWQAACKK-PSAMLLVPGNKKFVVNNLFFNGPCQPH 116

Query: 126 LMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHK 185
              +VDG +     P +W  N   R WL F ++ G +L G GVIDG+G++WW   C   K
Sbjct: 117 FTFKVDGIIAAYQNPASWKNN---RIWLQFAKLTGFTLMGKGVIDGQGKQWWAGQC---K 176

Query: 186 GLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGL 245
            +NG  +    D P AI+F  S+ L +QGLK+ NSP+FH  F  C+ V I  I I +P  
Sbjct: 177 WVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPRD 236

Query: 246 SPNTDGIHIENTNGVEIYNSVIFNGDDCVSIGSGAYDVDIRNITCGPSHGISIGSLGVRN 305
           SPNTDGI I  +    +  + I  GDDCV+IG+G+ ++ I ++ CGP HGISIGSLG  N
Sbjct: 237 SPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGREN 296

Query: 306 SRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYYCLT 365
           SRA V+ + V  +    + NG+RIKTWQGG G    + + N+ M N  NPI+I+Q+YC +
Sbjct: 297 SRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCTS 356

Query: 366 KD-CTNQTSAVSISDIQYTNIKGTYDIRSPPMHFGCSDSVPCTNLTLSEIELLPAQGDI 424
              C NQ SAV I D+ Y NI+GT    +  +   CSDS+PC ++ LS+I L    G I
Sbjct: 357 ASACQNQRSAVQIQDVTYKNIRGT-SATAAAIQLKCSDSMPCKDIKLSDISLKLTSGKI 407

BLAST of Lcy05g005020 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 8.1e-82
Identity = 161/384 (41.93%), Postives = 225/384 (58.59%), Query Frame = 0

Query: 69  DVRAFGAIGDGVVDDTEAFKMAWDTACQSDDSAVMLVPYGFSFMIQSTIFTGPCHGHLML 128
           +V  FGA GDG  DDT+AF+ AW  AC S  SAV+LVP   +++++   F+GPC   L +
Sbjct: 91  NVDDFGAKGDG-RDDTKAFEKAWKAACSSTSSAVLLVPKK-NYLVRPISFSGPCKSGLTM 150

Query: 129 RVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKGLN 188
           ++ GT+   D    + K+   R WLVF  +  + +EGGG I+G G+ WW   CK +K L 
Sbjct: 151 QIYGTIEASDDRSDYRKDG--RHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKTNKAL- 210

Query: 189 GTTLPGPC-DSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLSP 248
                 PC D+P A+ F+ S ++ V+ LKI N+ Q H  FD C NV   ++ + +P  SP
Sbjct: 211 ------PCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSP 270

Query: 249 NTDGIHIENTNGVEIYNSVIFNGDDCVSIGSGAYDVDIRNITCGPSHGISIGSLGVRNSR 308
           NTDGIH+  T  + I + VI  GDDC+SI +G+  V + +ITCGP HGISIGSLG  NS 
Sbjct: 271 NTDGIHVTGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSE 330

Query: 309 ACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYYC-LTK 368
           A V+++ V  + +  + NGVRIKTWQGG G+   + F N+ M NV NPIIIDQ YC   K
Sbjct: 331 AHVSDVVVNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDK 390

Query: 369 DCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFGCSDSVPCTNLTLSEIELLPAQGDIVLD 428
            C  Q+SAV + ++ Y NIKGT    +  + F CS   PC  + L +++ L  +G     
Sbjct: 391 PCQEQSSAVQVKNVVYQNIKGTC-ASNVAITFDCSKRFPCQGIVLEDVD-LEIEGGAAAK 450

Query: 429 PFCWNAYGDLQTITIPPVSCLQEG 451
             C N   +L    +    C +EG
Sbjct: 451 ALCNNV--ELSETGVVSPHCQEEG 459

BLAST of Lcy05g005020 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 1.4e-81
Identity = 157/356 (44.10%), Postives = 217/356 (60.96%), Query Frame = 0

Query: 67  MFDVRAFGAIGDGVVDDTEAFKMAWDTACQSDDSAVMLVPYGFSFMIQSTIFTGPCHGHL 126
           +F+V  +GA+GDG  D TEAF   W+ AC+   SAV+LVP    F + + +F GPC  HL
Sbjct: 58  VFNVEQYGAVGDGKHDSTEAFATTWNAACKK-ASAVLLVPANKKFFVNNLVFRGPCQPHL 117

Query: 127 MLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKG 186
             +VDGT++    P  W KN+  + WL F ++   +L G GVIDG+GQ+WW   CK    
Sbjct: 118 SFKVDGTIVAQPDPARW-KNS--KIWLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKV--- 177

Query: 187 LNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLS 246
           +NG T+    + P AI+   S ++TV+ L + NSP+FH  F  C+ V I  + IK+P  S
Sbjct: 178 VNGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPRDS 237

Query: 247 PNTDGIHIENTNGVEIYNSVIFNGDDCVSIGSGAYDVDIRNITCGPSHGISIGSLGVRNS 306
           PNTDGI I  +    I   VI  GDDC++IG+G+ ++ I+++ CGP HGISIGSLG  NS
Sbjct: 238 PNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRDNS 297

Query: 307 RACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYYCLTK 366
           RA V+++ V  +    + NG+RIKTWQGG G    +T+ N+ M N  NPI+I+Q+YC + 
Sbjct: 298 RAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSA 357

Query: 367 D-CTNQTSAVSISDIQYTNIKGTYDIRSPPMHFGCSDSVPCTNLTLSEIELLPAQG 422
             C NQ SAV I  + Y NI GT    +  +   CSDSVPCT + LS + L    G
Sbjct: 358 SACQNQRSAVQIQGVTYKNIHGT-SATAAAIQLMCSDSVPCTGIQLSNVSLKLTSG 405

BLAST of Lcy05g005020 vs. ExPASy TrEMBL
Match: A0A5D3BFC7 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold670G00080 PE=3 SV=1)

HSP 1 Score: 921.8 bits (2381), Expect = 1.2e-264
Identity = 435/467 (93.15%), Postives = 449/467 (96.15%), Query Frame = 0

Query: 1   MSSNFVLSIAFWFSFVFCLLSSSQARWHHHTKQKHSHHHKHSYISHPP--SPSPSDSPDD 60
           MSS  +LS A W SFV CLLSSSQARWHHHTKQKHSHHH+HSYISHPP  SPSPSDSPDD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPPSLSPSPSDSPDD 60

Query: 61  GDYIGETNMFDVRAFGAIGDGVVDDTEAFKMAWDTACQS-DDSAVMLVPYGFSFMIQSTI 120
           GDYI +TN+FDVRAFGAIGDG+VDDTEAFKMAWDTACQS DDSAVMLVPYGFSFMIQSTI
Sbjct: 61  GDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180
           FTGPCHG+LMLRVDGTLMPPDGPDAWPKN+SRRQWLVFYRIDGMSLEGGGVIDGRGQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDS 240
           DLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK VHIDS
Sbjct: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVIFNGDDCVSIGSGAYDVDIRNITCGPSHGIS 300
           IHIKSPGLSPNTDGIHIENTNGVEIYNSVI NGDDCVSIGSG+YD+DIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRG+TFSNIHMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFGCSDSVPCTNLTLSEIELL 420
           IDQ+YCLTKDCTNQTSAVS+SDIQYTNIKGTYDIRSPPMHF CSDSVPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPFCWNAYGDLQTITIPPVSCLQEGLPSSIDLNYDNEYC 465
           PAQGDIVLDPFCWNAYGDLQT+TIPPVSCLQEGLPSSI+ N DNEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of Lcy05g005020 vs. ExPASy TrEMBL
Match: A0A1S3CFX8 (polygalacturonase At1g48100-like OS=Cucumis melo OX=3656 GN=LOC103499990 PE=3 SV=1)

HSP 1 Score: 920.2 bits (2377), Expect = 3.4e-264
Identity = 434/467 (92.93%), Postives = 448/467 (95.93%), Query Frame = 0

Query: 1   MSSNFVLSIAFWFSFVFCLLSSSQARWHHHTKQKHSHHHKHSYISHPP--SPSPSDSPDD 60
           MSS  +LS A W SFV CLLSSSQARWHHHTKQKHSHHH+H YISHPP  SPSPSDSPDD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHGYISHPPSLSPSPSDSPDD 60

Query: 61  GDYIGETNMFDVRAFGAIGDGVVDDTEAFKMAWDTACQS-DDSAVMLVPYGFSFMIQSTI 120
           GDYI +TN+FDVRAFGAIGDG+VDDTEAFKMAWDTACQS DDSAVMLVPYGFSFMIQSTI
Sbjct: 61  GDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180
           FTGPCHG+LMLRVDGTLMPPDGPDAWPKN+SRRQWLVFYRIDGMSLEGGGVIDGRGQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDS 240
           DLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK VHIDS
Sbjct: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVIFNGDDCVSIGSGAYDVDIRNITCGPSHGIS 300
           IHIKSPGLSPNTDGIHIENTNGVEIYNSVI NGDDCVSIGSG+YD+DIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRG+TFSNIHMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFGCSDSVPCTNLTLSEIELL 420
           IDQ+YCLTKDCTNQTSAVS+SDIQYTNIKGTYDIRSPPMHF CSDSVPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPFCWNAYGDLQTITIPPVSCLQEGLPSSIDLNYDNEYC 465
           PAQGDIVLDPFCWNAYGDLQT+TIPPVSCLQEGLPSSI+ N DNEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of Lcy05g005020 vs. ExPASy TrEMBL
Match: A0A0A0KBA7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G414430 PE=3 SV=1)

HSP 1 Score: 910.2 bits (2351), Expect = 3.5e-261
Identity = 430/467 (92.08%), Postives = 445/467 (95.29%), Query Frame = 0

Query: 1   MSSNFVLSIAFWFSFVFCLLSSSQARWHHHTKQKHSHHHKHSYISHPP--SPSPSDSPDD 60
           MSS  +LS A W SFV CLLSSSQARWHHHTKQKHSH H+HSYISHPP  SPSPSDSPDD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHRHRHSYISHPPSLSPSPSDSPDD 60

Query: 61  GDYIGETNMFDVRAFGAIGDGVVDDTEAFKMAWDTACQS-DDSAVMLVPYGFSFMIQSTI 120
           G YI +TN+FDVRAFGAIGDG+VDDTEAFKMAWDTACQS DDS VMLVPYGFSFMIQSTI
Sbjct: 61  GYYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180
           FTGPCHG+LMLRVDGTLM PDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDS 240
           DLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK VHIDS
Sbjct: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVIFNGDDCVSIGSGAYDVDIRNITCGPSHGIS 300
           IHIKSPGLSPNTDGIHIENTNGV+IYNSVI NGDDCVSIGSG+YD+DIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRG+TFSNIHMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFGCSDSVPCTNLTLSEIELL 420
           IDQ+YCLTKDCTNQTSAVS+SDIQYTNIKGTYDIRSPPMHF CSDSVPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPFCWNAYGDLQTITIPPVSCLQEGLPSSIDLNYDNEYC 465
           PAQGDIVLDPFCWNAYGDLQT+TIPPVSCLQEGLPSS++ N DNEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 467

BLAST of Lcy05g005020 vs. ExPASy TrEMBL
Match: A0A6J1JNI5 (polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111488489 PE=3 SV=1)

HSP 1 Score: 901.7 bits (2329), Expect = 1.2e-258
Identity = 419/462 (90.69%), Postives = 441/462 (95.45%), Query Frame = 0

Query: 3   SNFVLSIAFWFSFVFCLLSSSQARWHHHTKQKHSHHHKHSYISHPPSPSPSDSPDDGDYI 62
           SNFVLS++FW +F+ CLL SSQARWHHH+KQKHSHHHK SYISHPP+PSP  SP DGDYI
Sbjct: 2   SNFVLSLSFWVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSP--SPGDGDYI 61

Query: 63  GETNMFDVRAFGAIGDGVVDDTEAFKMAWDTACQSDDSAVMLVPYGFSFMIQSTIFTGPC 122
            ETN+FDVR FGA+GDGV DDTEAFKMAWDTACQSDD+AV+LVPYG+SFMIQSTIFTGPC
Sbjct: 62  NETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDDAAVVLVPYGYSFMIQSTIFTGPC 121

Query: 123 HGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCK 182
           HGHLML VDGTLMPPDGPDAWP+NASRRQWLVFYRIDGMSLEGGGV+DGRGQKWWDLPCK
Sbjct: 122 HGHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCK 181

Query: 183 PHKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKS 242
           PHKG NGTTLPGPCDSPIAIRFFMSSNLTVQGLK TNSPQFHFRFDGCKNVHI+SIHIKS
Sbjct: 182 PHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKS 241

Query: 243 PGLSPNTDGIHIENTNGVEIYNSVIFNGDDCVSIGSGAYDVDIRNITCGPSHGISIGSLG 302
           PGLSPNTDGIHIENTN V IYNS+I+NGDDCVSIGSG+YDVDIRNITCGPSHGISIGSLG
Sbjct: 242 PGLSPNTDGIHIENTNDVGIYNSIIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLG 301

Query: 303 VRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYY 362
           VRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRG+TFSNIHMDNVRNPIIIDQ+Y
Sbjct: 302 VRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFY 361

Query: 363 CLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFGCSDSVPCTNLTLSEIELLPAQGD 422
           CLTKDCTNQTSAVSISDI+YTNIKGTYDIRS PMH  CSD+VPCTNLTLSEIELLPAQGD
Sbjct: 362 CLTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGD 421

Query: 423 IVLDPFCWNAYGDLQTITIPPVSCLQEGLPSSIDLNYDNEYC 465
           IVLDPFCWNAYGDLQT+TIPPVSCLQEGLPSSI LN DNEYC
Sbjct: 422 IVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

BLAST of Lcy05g005020 vs. ExPASy TrEMBL
Match: A0A6J1GP58 (polygalacturonase At1g48100 OS=Cucurbita moschata OX=3662 GN=LOC111456199 PE=3 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 2.1e-258
Identity = 419/462 (90.69%), Postives = 440/462 (95.24%), Query Frame = 0

Query: 3   SNFVLSIAFWFSFVFCLLSSSQARWHHHTKQKHSHHHKHSYISHPPSPSPSDSPDDGDYI 62
           SNFVLS++FW +F+ CLL SSQARWHHH+KQKHSHHHK SYISHPP+PSP  SP DGDYI
Sbjct: 2   SNFVLSLSFWVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSP--SPGDGDYI 61

Query: 63  GETNMFDVRAFGAIGDGVVDDTEAFKMAWDTACQSDDSAVMLVPYGFSFMIQSTIFTGPC 122
            ETN+FDVR FGA+GDGV DDTEAFKMAWD ACQSDD+AV+LVPYG+SFMIQSTIFTGPC
Sbjct: 62  NETNIFDVRGFGAVGDGVADDTEAFKMAWDMACQSDDAAVVLVPYGYSFMIQSTIFTGPC 121

Query: 123 HGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCK 182
           HGHLML VDGTLMPPDGPDAWP+NASRRQWLVFYRIDGMSLEGGGV+DGRGQKWWDLPCK
Sbjct: 122 HGHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCK 181

Query: 183 PHKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKS 242
           PHKG NGTTLPGPCDSPIAIRFFMSSNLTVQGLK TNSPQFHFRFDGCKNVHI+SIHIKS
Sbjct: 182 PHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKS 241

Query: 243 PGLSPNTDGIHIENTNGVEIYNSVIFNGDDCVSIGSGAYDVDIRNITCGPSHGISIGSLG 302
           PG SPNTDGIHIENTNGV IYNSVI+NGDDCVSIGSG+YDVDIRNITCGPSHGISIGSLG
Sbjct: 242 PGFSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLG 301

Query: 303 VRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYY 362
           VRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRG+TFSNIHMDNVRNPIIIDQ+Y
Sbjct: 302 VRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFY 361

Query: 363 CLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFGCSDSVPCTNLTLSEIELLPAQGD 422
           CLTKDCTNQTSAVSISDI+YTNIKGTYDIRS PMH  CSD+VPCTNLTLSEIELLPAQGD
Sbjct: 362 CLTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGD 421

Query: 423 IVLDPFCWNAYGDLQTITIPPVSCLQEGLPSSIDLNYDNEYC 465
           IVLDPFCWNAYGDLQT+TIPPVSCLQEGLPSSI LN DNEYC
Sbjct: 422 IVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

BLAST of Lcy05g005020 vs. NCBI nr
Match: XP_038899064.1 (polygalacturonase At1g48100-like [Benincasa hispida])

HSP 1 Score: 927.9 bits (2397), Expect = 3.3e-266
Identity = 438/468 (93.59%), Postives = 453/468 (96.79%), Query Frame = 0

Query: 3   SNFVLSIAFWFSFVFCLL-SSSQARWHHHTKQKHSHHHKHSYISHP----PSPSPSDS-P 62
           S+FVLS A WFSF+ CLL SSSQARWHHHTKQKHSHHHKHSYISHP    PSPSPSDS P
Sbjct: 2   SSFVLSFAIWFSFISCLLSSSSQARWHHHTKQKHSHHHKHSYISHPPSLSPSPSPSDSPP 61

Query: 63  DDGDYIGETNMFDVRAFGAIGDGVVDDTEAFKMAWDTACQSDDSAVMLVPYGFSFMIQST 122
           DDGDYI ETN+FDVRAFGAIG+GVVDDTEAFKMAWDTACQSDDSA+MLVPYG+SFMIQST
Sbjct: 62  DDGDYINETNIFDVRAFGAIGNGVVDDTEAFKMAWDTACQSDDSAIMLVPYGYSFMIQST 121

Query: 123 IFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKW 182
           IFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKW
Sbjct: 122 IFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKW 181

Query: 183 WDLPCKPHKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHID 242
           WDLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHID
Sbjct: 182 WDLPCKPHKGRNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHID 241

Query: 243 SIHIKSPGLSPNTDGIHIENTNGVEIYNSVIFNGDDCVSIGSGAYDVDIRNITCGPSHGI 302
           SIHIKSPGLSPNTDGIHIENTNGVEIYNSVI+NGDDCVSIGSG+YD+DIRNITCGPSHGI
Sbjct: 242 SIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDLDIRNITCGPSHGI 301

Query: 303 SIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPI 362
           SIGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRG+TFSNIHMDNVRNPI
Sbjct: 302 SIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPI 361

Query: 363 IIDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFGCSDSVPCTNLTLSEIEL 422
           IIDQ+YCLTKDC NQTSAVS+SDIQYTNIKGTYDIRSPPMHF CSDSVPCTNLTLSEIEL
Sbjct: 362 IIDQFYCLTKDCINQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIEL 421

Query: 423 LPAQGDIVLDPFCWNAYGDLQTITIPPVSCLQEGLPSSIDLNYDNEYC 465
           LP+QGDIVLDPFCWNAYGDLQT+TIPPVSCLQEGLPSSI+LN DNEYC
Sbjct: 422 LPSQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDNEYC 469

BLAST of Lcy05g005020 vs. NCBI nr
Match: TYJ96898.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 921.8 bits (2381), Expect = 2.4e-264
Identity = 435/467 (93.15%), Postives = 449/467 (96.15%), Query Frame = 0

Query: 1   MSSNFVLSIAFWFSFVFCLLSSSQARWHHHTKQKHSHHHKHSYISHPP--SPSPSDSPDD 60
           MSS  +LS A W SFV CLLSSSQARWHHHTKQKHSHHH+HSYISHPP  SPSPSDSPDD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHSYISHPPSLSPSPSDSPDD 60

Query: 61  GDYIGETNMFDVRAFGAIGDGVVDDTEAFKMAWDTACQS-DDSAVMLVPYGFSFMIQSTI 120
           GDYI +TN+FDVRAFGAIGDG+VDDTEAFKMAWDTACQS DDSAVMLVPYGFSFMIQSTI
Sbjct: 61  GDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180
           FTGPCHG+LMLRVDGTLMPPDGPDAWPKN+SRRQWLVFYRIDGMSLEGGGVIDGRGQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDS 240
           DLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK VHIDS
Sbjct: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVIFNGDDCVSIGSGAYDVDIRNITCGPSHGIS 300
           IHIKSPGLSPNTDGIHIENTNGVEIYNSVI NGDDCVSIGSG+YD+DIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRG+TFSNIHMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFGCSDSVPCTNLTLSEIELL 420
           IDQ+YCLTKDCTNQTSAVS+SDIQYTNIKGTYDIRSPPMHF CSDSVPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPFCWNAYGDLQTITIPPVSCLQEGLPSSIDLNYDNEYC 465
           PAQGDIVLDPFCWNAYGDLQT+TIPPVSCLQEGLPSSI+ N DNEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of Lcy05g005020 vs. NCBI nr
Match: XP_008461394.1 (PREDICTED: polygalacturonase At1g48100-like [Cucumis melo])

HSP 1 Score: 920.2 bits (2377), Expect = 7.0e-264
Identity = 434/467 (92.93%), Postives = 448/467 (95.93%), Query Frame = 0

Query: 1   MSSNFVLSIAFWFSFVFCLLSSSQARWHHHTKQKHSHHHKHSYISHPP--SPSPSDSPDD 60
           MSS  +LS A W SFV CLLSSSQARWHHHTKQKHSHHH+H YISHPP  SPSPSDSPDD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHHHRHGYISHPPSLSPSPSDSPDD 60

Query: 61  GDYIGETNMFDVRAFGAIGDGVVDDTEAFKMAWDTACQS-DDSAVMLVPYGFSFMIQSTI 120
           GDYI +TN+FDVRAFGAIGDG+VDDTEAFKMAWDTACQS DDSAVMLVPYGFSFMIQSTI
Sbjct: 61  GDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180
           FTGPCHG+LMLRVDGTLMPPDGPDAWPKN+SRRQWLVFYRIDGMSLEGGGVIDGRGQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDS 240
           DLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK VHIDS
Sbjct: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVIFNGDDCVSIGSGAYDVDIRNITCGPSHGIS 300
           IHIKSPGLSPNTDGIHIENTNGVEIYNSVI NGDDCVSIGSG+YD+DIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRG+TFSNIHMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFGCSDSVPCTNLTLSEIELL 420
           IDQ+YCLTKDCTNQTSAVS+SDIQYTNIKGTYDIRSPPMHF CSDSVPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPFCWNAYGDLQTITIPPVSCLQEGLPSSIDLNYDNEYC 465
           PAQGDIVLDPFCWNAYGDLQT+TIPPVSCLQEGLPSSI+ N DNEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of Lcy05g005020 vs. NCBI nr
Match: XP_004135975.1 (polygalacturonase At1g48100 [Cucumis sativus] >KGN45046.1 hypothetical protein Csa_016395 [Cucumis sativus])

HSP 1 Score: 910.2 bits (2351), Expect = 7.2e-261
Identity = 430/467 (92.08%), Postives = 445/467 (95.29%), Query Frame = 0

Query: 1   MSSNFVLSIAFWFSFVFCLLSSSQARWHHHTKQKHSHHHKHSYISHPP--SPSPSDSPDD 60
           MSS  +LS A W SFV CLLSSSQARWHHHTKQKHSH H+HSYISHPP  SPSPSDSPDD
Sbjct: 1   MSSFVLLSFAIWLSFVSCLLSSSQARWHHHTKQKHSHRHRHSYISHPPSLSPSPSDSPDD 60

Query: 61  GDYIGETNMFDVRAFGAIGDGVVDDTEAFKMAWDTACQS-DDSAVMLVPYGFSFMIQSTI 120
           G YI +TN+FDVRAFGAIGDG+VDDTEAFKMAWDTACQS DDS VMLVPYGFSFMIQSTI
Sbjct: 61  GYYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGFSFMIQSTI 120

Query: 121 FTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180
           FTGPCHG+LMLRVDGTLM PDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWW
Sbjct: 121 FTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWW 180

Query: 181 DLPCKPHKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDS 240
           DLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDGCK VHIDS
Sbjct: 181 DLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDGCKTVHIDS 240

Query: 241 IHIKSPGLSPNTDGIHIENTNGVEIYNSVIFNGDDCVSIGSGAYDVDIRNITCGPSHGIS 300
           IHIKSPGLSPNTDGIHIENTNGV+IYNSVI NGDDCVSIGSG+YD+DIRNITCGPSHGIS
Sbjct: 241 IHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNITCGPSHGIS 300

Query: 301 IGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPII 360
           IGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRG+TFSNIHMDNVRNPII
Sbjct: 301 IGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPII 360

Query: 361 IDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFGCSDSVPCTNLTLSEIELL 420
           IDQ+YCLTKDCTNQTSAVS+SDIQYTNIKGTYDIRSPPMHF CSDSVPCTNLTLSEIELL
Sbjct: 361 IDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 420

Query: 421 PAQGDIVLDPFCWNAYGDLQTITIPPVSCLQEGLPSSIDLNYDNEYC 465
           PAQGDIVLDPFCWNAYGDLQT+TIPPVSCLQEGLPSS++ N DNEYC
Sbjct: 421 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 467

BLAST of Lcy05g005020 vs. NCBI nr
Match: XP_022992012.1 (polygalacturonase At1g48100 [Cucurbita maxima])

HSP 1 Score: 901.7 bits (2329), Expect = 2.6e-258
Identity = 419/462 (90.69%), Postives = 441/462 (95.45%), Query Frame = 0

Query: 3   SNFVLSIAFWFSFVFCLLSSSQARWHHHTKQKHSHHHKHSYISHPPSPSPSDSPDDGDYI 62
           SNFVLS++FW +F+ CLL SSQARWHHH+KQKHSHHHK SYISHPP+PSP  SP DGDYI
Sbjct: 2   SNFVLSLSFWVTFLCCLLCSSQARWHHHSKQKHSHHHKRSYISHPPTPSP--SPGDGDYI 61

Query: 63  GETNMFDVRAFGAIGDGVVDDTEAFKMAWDTACQSDDSAVMLVPYGFSFMIQSTIFTGPC 122
            ETN+FDVR FGA+GDGV DDTEAFKMAWDTACQSDD+AV+LVPYG+SFMIQSTIFTGPC
Sbjct: 62  NETNIFDVRGFGAVGDGVADDTEAFKMAWDTACQSDDAAVVLVPYGYSFMIQSTIFTGPC 121

Query: 123 HGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCK 182
           HGHLML VDGTLMPPDGPDAWP+NASRRQWLVFYRIDGMSLEGGGV+DGRGQKWWDLPCK
Sbjct: 122 HGHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDGRGQKWWDLPCK 181

Query: 183 PHKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKS 242
           PHKG NGTTLPGPCDSPIAIRFFMSSNLTVQGLK TNSPQFHFRFDGCKNVHI+SIHIKS
Sbjct: 182 PHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCKNVHIESIHIKS 241

Query: 243 PGLSPNTDGIHIENTNGVEIYNSVIFNGDDCVSIGSGAYDVDIRNITCGPSHGISIGSLG 302
           PGLSPNTDGIHIENTN V IYNS+I+NGDDCVSIGSG+YDVDIRNITCGPSHGISIGSLG
Sbjct: 242 PGLSPNTDGIHIENTNDVGIYNSIIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLG 301

Query: 303 VRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYY 362
           VRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAVRG+TFSNIHMDNVRNPIIIDQ+Y
Sbjct: 302 VRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDNVRNPIIIDQFY 361

Query: 363 CLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFGCSDSVPCTNLTLSEIELLPAQGD 422
           CLTKDCTNQTSAVSISDI+YTNIKGTYDIRS PMH  CSD+VPCTNLTLSEIELLPAQGD
Sbjct: 362 CLTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTLSEIELLPAQGD 421

Query: 423 IVLDPFCWNAYGDLQTITIPPVSCLQEGLPSSIDLNYDNEYC 465
           IVLDPFCWNAYGDLQT+TIPPVSCLQEGLPSSI LN DNEYC
Sbjct: 422 IVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

BLAST of Lcy05g005020 vs. TAIR 10
Match: AT1G02460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 653.3 bits (1684), Expect = 1.5e-187
Identity = 306/467 (65.52%), Postives = 374/467 (80.09%), Query Frame = 0

Query: 12  WFSFVFCLLSSSQARWHHH-TKQKHSHHHKH--------SYISHPPSPSPS--------- 71
           + + +  L+S S+AR HHH  K KH++H+ H        S IS PP+P P          
Sbjct: 14  FMTLILTLMSLSEARNHHHKEKHKHNNHNHHSSKPKPPSSSISQPPTPPPGPPDSPAPSL 73

Query: 72  -DSPDDGDYIGETNMFDVRAFGAIGDGVVDDTEAFKMAWDTACQSDDS--AVMLVPYGFS 131
             SP D        +++VR +GA+GDG  DDTEAFK AWD++C ++++  +V+LVPYG++
Sbjct: 74  PPSPSDDPADDNNGIYNVRKYGAVGDGETDDTEAFKTAWDSSCNNENNTDSVLLVPYGYT 133

Query: 132 FMIQSTIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVID 191
           FMIQSTIFTGPC  +   +VDGT++ PDGP++WP N S+RQWLVFYR++GM+L+G GVID
Sbjct: 134 FMIQSTIFTGPCRSYQFFQVDGTIVTPDGPESWPSNISKRQWLVFYRVNGMALKGEGVID 193

Query: 192 GRGQKWWDLPCKPHKGLNGTTL-PGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDG 251
           GRGQKWWDLPCKPH+ +N + +  GPCDSPIA+RFFMSSNL V+GL+I NSPQFHFRFDG
Sbjct: 194 GRGQKWWDLPCKPHRSVNKSAIVTGPCDSPIALRFFMSSNLRVEGLQIKNSPQFHFRFDG 253

Query: 252 CKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIFNGDDCVSIGSGAYDVDIRNIT 311
           C+ VH++S+HI +P LSPNTDGIHIEN+N V IYNS+I NGDDCVSIGSG+YDVDIRN+T
Sbjct: 254 CQGVHVESLHITAPPLSPNTDGIHIENSNSVTIYNSIISNGDDCVSIGSGSYDVDIRNLT 313

Query: 312 CGP-SHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIH 371
           CGP  HGISIGSLG  NSRACV+NITVRDSVIK SDNGVRIKTWQGG G+V G+TF+NIH
Sbjct: 314 CGPGGHGISIGSLGNHNSRACVSNITVRDSVIKYSDNGVRIKTWQGGSGSVSGVTFNNIH 373

Query: 372 MDNVRNPIIIDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFGCSDSVPCTN 431
           +D+VRNPIIIDQYYC+TKDC N+TSAV +SDI Y  IKGTYDIRSPPMHFGCSD+VPCTN
Sbjct: 374 VDSVRNPIIIDQYYCMTKDCANKTSAVFVSDIAYQGIKGTYDIRSPPMHFGCSDAVPCTN 433

Query: 432 LTLSEIELLPAQGDIVLDPFCWNAYGDLQTITIPPVSCLQEGLPSSI 456
           LTLS+IELLPA+G+IVLDPFCWNAYG  + ++IPPV CL    P  +
Sbjct: 434 LTLSDIELLPAKGEIVLDPFCWNAYGIAEELSIPPVWCLMSDPPKGL 480

BLAST of Lcy05g005020 vs. TAIR 10
Match: AT4G01890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 592.8 bits (1527), Expect = 2.4e-169
Identity = 282/450 (62.67%), Postives = 344/450 (76.44%), Query Frame = 0

Query: 17  FCLLS-SSQARWHHHTKQKHSHHHKHSYISHPPSPS-----PSDSPDDGDYIGETNMFDV 76
           F LLS  ++AR HH   Q             PPSPS     PS SP     +    ++DV
Sbjct: 21  FSLLSYGTEARLHHQASQ-------------PPSPSPNPNDPSKSPSRSQDLDHEVVYDV 80

Query: 77  RAFGAIGDGVVDDTEAFKMAWDTAC---QSDDSAVMLVPYGFSFMIQSTIFTGPCHGHLM 136
           R +GA+G+GV DDT +FK AWD+AC   +++ ++V+ VPYGF+FMI+STIFTGPC  +  
Sbjct: 81  RKYGAVGNGVADDTVSFKTAWDSACSNNKNNTASVLHVPYGFTFMIRSTIFTGPCRSYQY 140

Query: 137 LRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPH-KG 196
            +VDGT++P DGP +WP + ++RQWL FYRI+GM+L+G GVIDGRGQ WWDLPCKPH + 
Sbjct: 141 FQVDGTIVPRDGPKSWPSSLNKRQWLAFYRINGMALQGAGVIDGRGQNWWDLPCKPHQQN 200

Query: 197 LNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLS 256
           +N T L GPC+SP A+RFFMSSN+ V+GL I NSPQ H + DGC  VHI+S+ I SP  S
Sbjct: 201 VNKTKLAGPCESPAALRFFMSSNVIVKGLSIKNSPQVHLKLDGCHVVHINSLRIISPPAS 260

Query: 257 PNTDGIHIENTNGVEIYNSVIFNGDDCVSIGSGAYDVDIRNITCGP-SHGISIGSLGVRN 316
           PNTDGIHIEN+N VEIYNSVI NGDDCVSIG GAYD+DIRNITCGP  HGISIGSLG +N
Sbjct: 261 PNTDGIHIENSNSVEIYNSVISNGDDCVSIGPGAYDIDIRNITCGPGGHGISIGSLGEKN 320

Query: 317 SRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYYCLT 376
           S ACV+N+TVRDS IK S+NGVRIKTWQGG G+V G+TF NIH+D VRNPIIIDQYYC T
Sbjct: 321 SHACVSNVTVRDSFIKFSENGVRIKTWQGGSGSVSGVTFDNIHVDTVRNPIIIDQYYCTT 380

Query: 377 KDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFGCSDSVPCTNLTLSEIELLPAQGDIVL 436
           K C N+TSAV ++DI Y +IKGTYDIRSPPMHFGCS++VPCTNLTLS IELLP++ DIV+
Sbjct: 381 KSCANKTSAVFVNDIVYQSIKGTYDIRSPPMHFGCSNNVPCTNLTLSNIELLPSKEDIVV 440

Query: 437 DPFCWNAYGDLQTITIPPVSCLQEGLPSSI 456
            PFCWNAYG     ++P +SCL+    +S+
Sbjct: 441 GPFCWNAYGITDEFSVPLISCLKSNPSTSL 457

BLAST of Lcy05g005020 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 568.9 bits (1465), Expect = 3.7e-162
Identity = 260/407 (63.88%), Postives = 326/407 (80.10%), Query Frame = 0

Query: 48  PSPSPSDSPDDGDYIGETNMFDVRAFGAIGDGVVDDTEAFKMAWDTACQS--DDSAVMLV 107
           PSP+P+ + +D D I  T +FDV +FGAIGD   DDT AFKMAWD AC S    SA++LV
Sbjct: 29  PSPAPNPAYNDNDNIAPT-VFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGPKSALLLV 88

Query: 108 PYGFSFMIQSTIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEG 167
           PY F F+++ T F GPC  +L+L++DG ++ PDGP +WP N  +RQW++FYR++G+S++G
Sbjct: 89  PYTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWPSN-YQRQWMMFYRVNGLSIQG 148

Query: 168 GGVIDGRGQKWWDLPCKPHKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHF 227
            GVI+GRGQKWW+LPCKPHKGLNGTT  GPCDSP+AIR F SS + +QG+   NS QFH 
Sbjct: 149 SGVINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSKVRIQGINFMNSAQFHV 208

Query: 228 RFDGCKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIFNGDDCVSIGSGAYDVDI 287
           RFD C +V +DS+ IK+P  SPNTDGIHIENT+ V+I NS+I NGDDC+SIG+G ++VDI
Sbjct: 209 RFDNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDDCISIGAGCFNVDI 268

Query: 288 RNITCGPSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFS 347
           +N+TCGPSHGISIGSLGV NS+A V+NITV +S I  SDNGVRIKTWQGG G+V  + FS
Sbjct: 269 KNVTCGPSHGISIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTWQGGSGSVSRIVFS 328

Query: 348 NIHMDNVRNPIIIDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFGCSDSVP 407
           NI M NVRNPI+IDQYYC T +C NQTSAV ISD+ Y NIKGTYD+RSPP+HFGCSDSVP
Sbjct: 329 NILMVNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTYDLRSPPIHFGCSDSVP 388

Query: 408 CTNLTLSEIELLPAQGDIVLDPFCWNAYGDLQTITIPPVSCLQEGLP 453
           CTNLTL+E++L P++G  + +PFCWNAYG ++ IT+PPV CL +  P
Sbjct: 389 CTNLTLTEVDLFPSKGQHLENPFCWNAYGSMK-ITVPPVYCLLDAPP 432

BLAST of Lcy05g005020 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 562.8 bits (1449), Expect = 2.6e-160
Identity = 261/455 (57.36%), Postives = 335/455 (73.63%), Query Frame = 0

Query: 23  SQARWHHHTKQKHSHHH--------------KHSYISHPPSPSPSDSPDDGDYIGETNMF 82
           ++AR+H+H K +H   H                  +   P PSP  +P D D      +F
Sbjct: 25  ARARYHYH-KGRHGVTHPLPPPPPPPLETANPPDQVPSDPYPSPDPAPGDSD---SGCVF 84

Query: 83  DVRAFGAIGDGVVDDTEAFKMAWDTACQSDDSAVMLVPYGFSFMIQSTIFTGPCHGHLML 142
           DV +FGA+GDG  DDT AF+ AW  AC + +S V+L P G  F I STIF+GPC   L+ 
Sbjct: 85  DVTSFGAVGDGSCDDTAAFQDAWKAAC-AVESGVVLAPEGGVFKITSTIFSGPCKPGLVF 144

Query: 143 RVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKGLN 202
           ++DG LMPPDGP+ WP+  ++ QWLVFYR+DG +  G G ++G GQKWWDLPCKPH+G +
Sbjct: 145 QLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWDLPCKPHRGPD 204

Query: 203 GTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLSPN 262
           G++  GPC SP  IRFFMS+N+ V+GL+I NSPQFH +FDGC+ V I+ I I SP LSPN
Sbjct: 205 GSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISSPKLSPN 264

Query: 263 TDGIHIENTNGVEIYNSVIFNGDDCVSIGSGAYDVDIRNITCGPSHGISIGSLGVRNSRA 322
           TDGIH+ NT  V IYNSV+ NGDDC+SIG+G  DVDI+ +TCGPSHGISIGSLGV NS+A
Sbjct: 265 TDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGISIGSLGVHNSQA 324

Query: 323 CVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQYYCLTKDC 382
           CV+NITVR++VI+ SDNG+R+KTWQGG G+V  L F NI M+NV N II+DQYYC +KDC
Sbjct: 325 CVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNCIIVDQYYCQSKDC 384

Query: 383 TNQTSAVSISDIQYTNIKGTYDIRSPPMHFGCSDSVPCTNLTLSEIELLPAQGDIVLDPF 442
            N+TSAV + D++Y NIKGTYD+RSPP+HF CSD+V CTN+T+SE+ELLP +G++V DPF
Sbjct: 385 RNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLPEEGELVDDPF 444

Query: 443 CWNAYGDLQTITIPPVSCLQEGLPSSIDLNYDNEY 464
           CWNAYG  +T+TIPP+ CL +G P  ++  YD+ Y
Sbjct: 445 CWNAYGKQETLTIPPIDCLLDGSP-VVEEAYDSNY 473

BLAST of Lcy05g005020 vs. TAIR 10
Match: AT5G14650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 377.1 bits (967), Expect = 2.0e-104
Identity = 182/404 (45.05%), Postives = 257/404 (63.61%), Query Frame = 0

Query: 63  GETNMFDVRAFGAIGDGVVDDTEAFKMAWDTACQSDDSAVMLVPYGFSFMIQSTIFTG-P 122
           G +   +V   GA GDG  DDT+AF+ AW  AC+   ++ +LVP G +F++    F G  
Sbjct: 46  GSSGTINVLDHGAKGDGTSDDTKAFEDAWQVACKV-AASTLLVPSGSTFLVGPVSFLGKE 105

Query: 123 CHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPC 182
           C   ++ +++G ++ P    AW   +   QW+ F  + G++++G G+IDGRG  WW+   
Sbjct: 106 CKEKIVFQLEGKIIAPTSASAW--GSGLLQWIEFKALQGITIKGKGIIDGRGSVWWN--- 165

Query: 183 KPHKGLNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIK 242
                + GT +P     P A+RF+ S+ +TV G+ I NSPQ H +FD C ++ +      
Sbjct: 166 ----DMMGTKMPR--TKPTALRFYGSNGVTVSGITIQNSPQTHLKFDNCISIQVSDFTTS 225

Query: 243 SPGLSPNTDGIHIENTNGVEIYNSVIFNGDDCVSIGSGAYDVDIRNITCGPSHGISIGSL 302
           SPG SPNTDGIH++N+    IY S +  GDDC+SI +G  +++I ++ CGP HGISIG L
Sbjct: 226 SPGDSPNTDGIHLQNSQDAVIYRSTLACGDDCISIQTGCSNINIHDVDCGPGHGISIGGL 285

Query: 303 GVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGLTFSNIHMDNVRNPIIIDQY 362
           G  N++ACV+NITVRD  +  + NGVRIK+WQGG G+V+ + FSNI + NV NPIIIDQY
Sbjct: 286 GKDNTKACVSNITVRDVTMHETTNGVRIKSWQGGSGSVKQVMFSNIQVSNVANPIIIDQY 345

Query: 363 YCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFGCSDSVPCTNLTLSEIELLPAQG 422
           YC    C N+TSAV++S+I Y NIKGTY     P+ F CSDS+PCT ++LS IEL PA G
Sbjct: 346 YCDGGGCHNETSAVAVSNINYINIKGTY--TKEPVRFACSDSLPCTGISLSTIELKPATG 405

Query: 423 DI-VLDPFCWNAYGDLQTITIPPVSCLQEGLPSSIDLNYDNEYC 465
               LDPFCW A+G+L+T T+PP+ CL+           +N+ C
Sbjct: 406 KASSLDPFCWKAHGELKTKTLPPIQCLKTEKSPEAASRSNNDAC 435

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949Z13.7e-15957.36Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV... [more]
Q94AJ53.5e-9342.64Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
P432126.7e-8444.01Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
P353368.1e-8241.93Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
Q7M1E71.4e-8144.10Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3BFC71.2e-26493.15Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold670G00... [more]
A0A1S3CFX83.4e-26492.93polygalacturonase At1g48100-like OS=Cucumis melo OX=3656 GN=LOC103499990 PE=3 SV... [more]
A0A0A0KBA73.5e-26192.08Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G414430 PE=3 SV=1[more]
A0A6J1JNI51.2e-25890.69polygalacturonase At1g48100 OS=Cucurbita maxima OX=3661 GN=LOC111488489 PE=3 SV=... [more]
A0A6J1GP582.1e-25890.69polygalacturonase At1g48100 OS=Cucurbita moschata OX=3662 GN=LOC111456199 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_038899064.13.3e-26693.59polygalacturonase At1g48100-like [Benincasa hispida][more]
TYJ96898.12.4e-26493.15polygalacturonase [Cucumis melo var. makuwa][more]
XP_008461394.17.0e-26492.93PREDICTED: polygalacturonase At1g48100-like [Cucumis melo][more]
XP_004135975.17.2e-26192.08polygalacturonase At1g48100 [Cucumis sativus] >KGN45046.1 hypothetical protein C... [more]
XP_022992012.12.6e-25890.69polygalacturonase At1g48100 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G02460.11.5e-18765.52Pectin lyase-like superfamily protein [more]
AT4G01890.12.4e-16962.67Pectin lyase-like superfamily protein [more]
AT1G56710.13.7e-16263.88Pectin lyase-like superfamily protein [more]
AT1G48100.12.6e-16057.36Pectin lyase-like superfamily protein [more]
AT5G14650.12.0e-10445.05Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 257..278
e-value: 260.0
score: 10.4
coord: 230..256
e-value: 94.0
score: 11.9
coord: 310..331
e-value: 14.0
score: 14.6
coord: 339..360
e-value: 1100.0
score: 6.9
coord: 280..300
e-value: 8800.0
score: 0.5
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 62..454
e-value: 5.4E-119
score: 399.8
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 95..416
e-value: 3.2E-87
score: 292.6
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 287..300
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 38..60
NoneNo IPR availablePANTHERPTHR31375:SF119POLYGALACTURONASE/GLYCOSIDE HYDROLASE FAMILY PROTEINcoord: 17..453
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 17..453
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 65..452

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy05g005020.1Lcy05g005020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0016829 lyase activity
molecular_function GO:0004650 polygalacturonase activity