Lcy05g003790 (gene) Sponge gourd (P93075) v1

BLAST of Lcy05g003790 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 5.0e-224
Identity = 388/540 (71.85%), Postives = 453/540 (83.89%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATGHVYSYDDVQ 60
           M  +T Q  + IFRSKLPDI+IPNHLPLH Y F+N ++F SRPCLIN A   +Y+Y DV+
Sbjct: 1   MEKDTKQV-DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVE 60

Query: 61  LTSRRVASGLHTLGIKKGDVVMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           L SR+VA+GLH  GI+  D +M LLPNSPEFVF F+ ASY GAI T ANP  T  E+ KQ
Sbjct: 61  LNSRKVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 120

Query: 121 AKAAYAKLIVTMACFYDKVKDLA-EDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEID 180
           AKA+ AK+IVT AC  +KVKD A E+ VKI+C+D A EGCLHFSVL+ A+E   P VEI 
Sbjct: 121 AKASSAKIIVTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQ 180

Query: 181 AEDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYDRDDVVLCVLPLFHIY 240
            +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY   +DV+LCVLPLFHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIY 240

Query: 241 SLNSILLCGLRAGAAILIMQKFEIVSLLHLVEKYKVSIMLIVPPILLAIAKSPEFEKYDV 300
           SLNS+LLCGLR GAAILIMQKF+IVS L L+++YKV+I   VPPI+LAIAKSP  + YD+
Sbjct: 241 SLNSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDL 300

Query: 301 SSVRILKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGS 360
           SSVR + SG APLGKELE+ VRAKFP+A LGQGYGMTEAGPVL M LAFAKEPF +K+G+
Sbjct: 301 SSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGA 360

Query: 361 CGTVVRNAEMKVVDPETGASLPANSTGEICIRGDQIMKGYLNDLESTKSTIDKEGWLHTG 420
           CGTVVRNAEMK+VDP+TG SLP N +GEICIRGDQIMKGYLND E+T  TIDKEGWL+TG
Sbjct: 361 CGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTG 420

Query: 421 DIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKADEQAGEVP 480
           DIG++DDDDELFIVDRLKELIK+K FQVAPAELEALL+ HP +SDAAV+   DEQAGEVP
Sbjct: 421 DIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVP 480

Query: 481 VAFVVKAEGDAIAEEDVKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASG 540
           VAFVV++ G  I E++VK FISKQV+FYKR+ RVFFV+ IPK+PSGKILRK+LRAKLA+G
Sbjct: 481 VAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAG 539

BLAST of Lcy05g003790 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 775.8 bits (2002), Expect = 3.2e-223
Identity = 381/540 (70.56%), Postives = 452/540 (83.70%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATGHVYSYDDVQ 60
           M  ETNQ  + IFRSKLPDI+IP HLPLH Y F+N ++F SRPCLIN A  H+Y+Y DV+
Sbjct: 3   METETNQ-GDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANNHIYTYADVE 62

Query: 61  LTSRRVASGLHTLGIKKGDVVMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           LTSR+VA+GL+ LGI++ D +M LLPNSPEFVF F+ ASY GAI T ANP  T  E+ KQ
Sbjct: 63  LTSRKVAAGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKAAYAKLIVTMACFYDKVKDLA-EDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEID 180
           AKA+ AKLI+T ACF +KVKD A ++ + ++C+D A EGC+HFS L+ ADE   P V+I 
Sbjct: 123 AKASNAKLIITQACFVNKVKDYAFDNNLNVICIDSAPEGCIHFSELTQADEHDIPDVKIQ 182

Query: 181 AEDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYDRDDVVLCVLPLFHIY 240
           ++DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY   +DV++CVLPLFHIY
Sbjct: 183 SDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIY 242

Query: 241 SLNSILLCGLRAGAAILIMQKFEIVSLLHLVEKYKVSIMLIVPPILLAIAKSPEFEKYDV 300
           SLNS+LLCGLR GAAILIMQKF+IV    L+EKYKV+I   VPPI+LAIAKSP  + YD+
Sbjct: 243 SLNSVLLCGLRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVVDNYDL 302

Query: 301 SSVRILKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGS 360
           SSVR + SG APLGKELE+AVR KFP+A LGQGYGMTEAGPVL M LAFAKEPF +K+G+
Sbjct: 303 SSVRTVMSGAAPLGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGA 362

Query: 361 CGTVVRNAEMKVVDPETGASLPANSTGEICIRGDQIMKGYLNDLESTKSTIDKEGWLHTG 420
           CGTVVRNAEMK+VDP+TG SLP N  GEICIRGDQIMKGYLND  +T  TIDKEGWLHTG
Sbjct: 363 CGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPAATTRTIDKEGWLHTG 422

Query: 421 DIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKADEQAGEVP 480
           DIG++D+DDELFIVDRLKELIK+K FQVAPAELEALL+ HP +SDAAV+   DEQAGEVP
Sbjct: 423 DIGYIDNDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVP 482

Query: 481 VAFVVKAEGDAIAEEDVKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASG 540
           VAFVV++ G  I E++VK F+SKQV+FYKR+ RVFFV T+PK+PSGKILRK+LRA+LA+G
Sbjct: 483 VAFVVRSNGSDITEDEVKDFVSKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAAG 541

BLAST of Lcy05g003790 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 7.2e-223
Identity = 381/537 (70.95%), Postives = 449/537 (83.61%), Query Frame = 0

Query: 4   ETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATGHVYSYDDVQLTS 63
           ET Q+ + IFRSKLPDI+IP HLPLH Y F+N ++F SRPCLIN A   +Y+Y +V+LT 
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVELTC 67

Query: 64  RRVASGLHTLGIKKGDVVMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQAKA 123
           R+VA GL+ LGI++ D +M LLPNSPEFVF F+ ASY GAI T ANP  T  E+ KQAKA
Sbjct: 68  RKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKA 127

Query: 124 AYAKLIVTMACFYDKVKDLA-EDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDAED 183
           + AK+I+T +CF  KVKD A E+ VK++C+D A EGCLHFS L+ +DE   P V+I  +D
Sbjct: 128 SSAKIIITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPDD 187

Query: 184 VVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYDRDDVVLCVLPLFHIYSLN 243
           VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+N NLY   +DV++CVLPLFHIYSLN
Sbjct: 188 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLN 247

Query: 244 SILLCGLRAGAAILIMQKFEIVSLLHLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVSSV 303
           SILLCGLR GAAILIMQKF+I   L L++KYKVSI   VPPI+LAIAKSP  + YD+SSV
Sbjct: 248 SILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSSV 307

Query: 304 RILKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGSCGT 363
           R + SG APLGKELE+AVR KFP+A LGQGYGMTEAGPVL M LAFAKEPF +K+G+CGT
Sbjct: 308 RTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGT 367

Query: 364 VVRNAEMKVVDPETGASLPANSTGEICIRGDQIMKGYLNDLESTKSTIDKEGWLHTGDIG 423
           VVRNAEMK+VDP+TG SLP N  GEICIRGDQIMKGYLND E+T  TIDKEGWLHTGDIG
Sbjct: 368 VVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDIG 427

Query: 424 FVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKADEQAGEVPVAF 483
           F+D+DDELFIVDRLKELIK+K FQVAPAE+EALL+ HP +SDAAV+   DEQAGEVPVAF
Sbjct: 428 FIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVAF 487

Query: 484 VVKAEGDAIAEEDVKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASG 540
           VV++ G AI E++VK FISKQV+FYKR+ RVFFV T+PK+PSGKILRK+LRA+LA+G
Sbjct: 488 VVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAG 544

BLAST of Lcy05g003790 vs. ExPASy Swiss-Prot
Match: P14913 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 1.2e-222
Identity = 380/531 (71.56%), Postives = 448/531 (84.37%), Query Frame = 0

Query: 10  EFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATGHVYSYDDVQLTSRRVASG 69
           + IFRSKLPDI+IP HLPLH Y F+N +K G + CLIN ATG  ++Y  V+L SR+VASG
Sbjct: 10  DLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVASG 69

Query: 70  LHTLGIKKGDVVMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQAKAAYAKLI 129
           L+ LGI++GD +M LLPNSPE+ F FL ASYRGAI T ANPF T+ E+ KQ KA+ AKLI
Sbjct: 70  LNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLAKLI 129

Query: 130 VTMACFYDKVKD-LAEDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDAEDVVALPY 189
           +T AC+ DKVKD  AE  ++I+C+D A + CLHFS L  ADES  P V ID++DVVALPY
Sbjct: 130 ITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVVIDSDDVVALPY 189

Query: 190 SSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYDRDDVVLCVLPLFHIYSLNSILLCG 249
           SSGTTGLPKGVMLTHKGL+TSVAQQ+DG NPNLY   +DV++C+LPLFHIYSLN++L CG
Sbjct: 190 SSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAVLCCG 249

Query: 250 LRAGAAILIMQKFEIVSLLHLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVSSVRILKSG 309
           LRAG  ILIMQKF+IV  L L++KYKV+I   VPPI+LAIAKSP  +KYD+SSVR + SG
Sbjct: 250 LRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRTVMSG 309

Query: 310 GAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGSCGTVVRNAE 369
            APLGKELE+AVRAKFP+A LGQGYGMTEAGPVL M LAFAKEP+ +K+G+CGTVVRNAE
Sbjct: 310 AAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRNAE 369

Query: 370 MKVVDPETGASLPANSTGEICIRGDQIMKGYLNDLESTKSTIDKEGWLHTGDIGFVDDDD 429
           MK+VDPET ASLP N  GEICIRGDQIMKGYLND EST++TID+EGWLHTGDIGF+DDDD
Sbjct: 370 MKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDDDD 429

Query: 430 ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKADEQAGEVPVAFVVKAEG 489
           ELFIVDRLKE+IK+K FQVAPAELEALL+THP +SDAAV+   DE+AGEVPVAFVV+  G
Sbjct: 430 ELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRTNG 489

Query: 490 DAIAEEDVKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASG 540
               EE++KQF+SKQVVFYKR+ RVFFV+ IPK+PSGKILRK+LRAK+ASG
Sbjct: 490 FTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRAKIASG 540

BLAST of Lcy05g003790 vs. ExPASy Swiss-Prot
Match: O24540 (4-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1)

HSP 1 Score: 773.9 bits (1997), Expect = 1.2e-222
Identity = 391/546 (71.61%), Postives = 453/546 (82.97%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATGHVYSYDDVQ 60
           +A+E  Q  + IFRSKLPDI+IP +LPLH Y F+N +KF SRPCLIN AT  +++Y DV+
Sbjct: 5   VAIE-EQKKDIIFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYADVE 64

Query: 61  LTSRRVASGLHTLGIKKGDVVMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           L SRRV SGL  LGIK+GD +M LLPNSPEFVF FL AS+ G+I T ANPF T+ E+ KQ
Sbjct: 65  LISRRVGSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQ 124

Query: 121 AKAAYAKLIVTMACFYDKVKDLA-EDGVKIVCVDLA-----VEGCLHFSVLSGADESHAP 180
           AKA+ AKLI+T  C+ DKVKD A E+GVKI+ +D           LHFS L+GADE+  P
Sbjct: 125 AKASNAKLIITQGCYVDKVKDYACENGVKIISIDTTTTADDAANILHFSELTGADENEMP 184

Query: 181 PVEIDAEDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYDRDDVVLCVLP 240
            VEI  + VVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY   DDV+LCVLP
Sbjct: 185 KVEISPDGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVLP 244

Query: 241 LFHIYSLNSILLCGLRAGAAILIMQKFEIVSLLHLVEKYKVSIMLIVPPILLAIAKSPEF 300
           LFHIYSLNS+LLCGLRAG+ ILIMQKFEIV  L L++KYKV+I   VPPI+LAIAKS   
Sbjct: 245 LFHIYSLNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTVV 304

Query: 301 EKYDVSSVRILKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLAFAKEPFP 360
           + YD+SSVR + SG APLGKELE+AVRAKFP+A LGQGYGMTEAGPVL M LAFAKEPF 
Sbjct: 305 DNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFD 364

Query: 361 VKAGSCGTVVRNAEMKVVDPETGASLPANSTGEICIRGDQIMKGYLNDLESTKSTIDKEG 420
           +K+G+CGTVVRNAEMK+VDPETG+SLP N  GEICIRGDQIMKGYLND E+T  TIDKEG
Sbjct: 365 IKSGACGTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKEG 424

Query: 421 WLHTGDIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKADEQ 480
           WLHTGDIG++DDDDELFIVDRLKELIK+K FQVAPAELEALL+THP +SDAAV+   DE 
Sbjct: 425 WLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDEA 484

Query: 481 AGEVPVAFVVKAEGDAIAEEDVKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRA 540
           AGEVPVAFVVK+ G  I E+++KQFISKQV+FYKR+NRVFFV  IPKAPSGKILRK+LRA
Sbjct: 485 AGEVPVAFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLRA 544

BLAST of Lcy05g003790 vs. ExPASy TrEMBL
Match: A0A6J1JN39 (4-coumarate--CoA ligase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111488383 PE=4 SV=1)

HSP 1 Score: 961.4 bits (2484), Expect = 1.5e-276
Identity = 486/542 (89.67%), Postives = 510/542 (94.10%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATGHVYSYDDVQ 60
           MAVE+NQT+EFIFRSKLPDIHIPNHLPLHEYVFQN +KFGSRPCLIN ATG +YSY DVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDIYSYHDVQ 60

Query: 61  LTSRRVASGLHTLGIKKGDVVMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           L SRRVA+GLH LGIKKGDVVMNLLPNSPEFVFTFL ASY GA+MTAANPF TAVEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAAYAKLIVTMACFYDKVKDLAEDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDA 180
           AKAA AKLIVTMACFYD+VKDLAE+GVKIVCVD AVEGCLH+SVLSGADESH PPVE  A
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPPVEFAA 180

Query: 181 EDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYDRDDVVLCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYY   DV+LCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLHLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVSLL L+EK+KVSIM IVPPI LAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRILKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGSC 360
           SVR+LKSGGAPLGKELE+AVR KFP+AVLGQGYGMTEAGPVLTMSLAFAKEPF VKAG+C
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFAVKAGAC 360

Query: 361 GTVVRNAEMKVVDPETGASLPANSTGEICIRGDQIMKGYLNDLESTKSTIDKEGWLHTGD 420
           GTVVRNAEMK+VDPETGASLPANS GEICIRG+QIMKGYLNDLESTK T+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKADEQAGEVPV 480
           IGF+DDDDELFIVDRLKELIKFKAFQVAPAELE+LLITHPKLSDAAVIG  DEQAGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKAEGDAIAEEDVKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKA G AI EEDVKQFISKQVVFYKRL RVFFVN IPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YH 543
           ++
Sbjct: 541 FN 542

BLAST of Lcy05g003790 vs. ExPASy TrEMBL
Match: A0A6J1GMS9 (4-coumarate--CoA ligase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111455820 PE=4 SV=1)

HSP 1 Score: 960.3 bits (2481), Expect = 3.4e-276
Identity = 486/542 (89.67%), Postives = 510/542 (94.10%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATGHVYSYDDVQ 60
           MAVE+NQT+EFIFRSKLPDIHIPNHLPLHEYVFQN +KFGSRPCLIN ATG VYSY DVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVASGLHTLGIKKGDVVMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           L SRRVA+GLH LGIKK DVVMNLLPNSPEFVFTFLAASY GA+MTAANPF TAVEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKSDVVMNLLPNSPEFVFTFLAASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAAYAKLIVTMACFYDKVKDLAEDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDA 180
           AKAA AKLIVTMACFYD+VKDLAE+GVKIVCVD AVEGCLH+SVLSGADESH P VE  A
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPAVEFAA 180

Query: 181 EDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYDRDDVVLCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYY   DV+LCVLP FH+YS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGSDVILCVLPFFHVYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLHLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEI+SLL L+EK+KVSIM IVPPI LAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEILSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRILKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGSC 360
           SVR+LKSGGAPLGKELE+AVR KFP+AVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAG+C
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKVVDPETGASLPANSTGEICIRGDQIMKGYLNDLESTKSTIDKEGWLHTGD 420
           GTVVRNAEMK+VDPETGASLPANS GEICIRG+QIMKGYLNDLESTK T+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKADEQAGEVPV 480
           IGFVDDDDELFIVDRLKELIKFKAFQVAPAELE+LLITHPKLSDAAVIG  DEQAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKAEGDAIAEEDVKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKA G AI EEDVKQFISKQVVFYKRL RVFFVN IPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YH 543
           ++
Sbjct: 541 FN 542

BLAST of Lcy05g003790 vs. ExPASy TrEMBL
Match: A0A6J1H7E7 (4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111460855 PE=4 SV=1)

HSP 1 Score: 954.9 bits (2467), Expect = 1.4e-274
Identity = 484/541 (89.46%), Postives = 504/541 (93.16%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATGHVYSYDDVQ 60
           MA E NQTNEFIFRSKLPDIHIPNHLPLH YVFQN +KFGSRPCLIN ATG VYSY DVQ
Sbjct: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVASGLHTLGIKKGDVVMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           LT+RRVASGLH LGIKKGDVVMNLLPNSPEFVFTFL ASYRGAIMTAANPF TAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAAYAKLIVTMACFYDKVKDLAEDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDA 180
           AKAA  KLIVTMACFYD+VKDLAE+GVKIVCVD AVE CLH+SVLSGADESH P V+  A
Sbjct: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEDCLHYSVLSGADESHLPFVDFAA 180

Query: 181 EDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYDRDDVVLCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG+NPNLYY   DV+LCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGENPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLHLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVS L LVEKYKVSIM IVPPI LAIAKSP+ EKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSFLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300

Query: 301 SVRILKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGSC 360
           SVR+LKSGGAPLGKELE+AVR KFP+A+LGQGYGMTEAGPVLTMSLAFAKEPFPVKAG+C
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKVVDPETGASLPANSTGEICIRGDQIMKGYLNDLESTKSTIDKEGWLHTGD 420
           GTVVRNAEMK+VDPETGASLPANS GEICIRGDQIMKGYLNDLESTK T+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKADEQAGEVPV 480
           IGF+D+DDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIG  DEQAGEVPV
Sbjct: 421 IGFIDNDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKAEGDAIAEEDVKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKA G AI EEDVKQFISKQVVFYKRL RVFFVN IPKAPSGKILRKELRAKLA+GA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLANGA 540

Query: 541 Y 542
           Y
Sbjct: 541 Y 541

BLAST of Lcy05g003790 vs. ExPASy TrEMBL
Match: A0A6J1KT15 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111497966 PE=4 SV=1)

HSP 1 Score: 950.7 bits (2456), Expect = 2.7e-273
Identity = 481/542 (88.75%), Postives = 504/542 (92.99%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATGHVYSYDDVQ 60
           MA E NQTNEFIFRSKLPDIHIPNHLPLH YVFQN +K GSRPCLIN ATG VYSY DVQ
Sbjct: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKLGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVASGLHTLGIKKGDVVMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           LT+RRVASGLH LGIKKG+VVMNLLPNSPEFVFTFL ASYRGAIMTAANPF TAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGNVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAAYAKLIVTMACFYDKVKDLAEDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDA 180
           AKAA  KLIVTMACFYD+VKDLAE+GVKIVCVD AVEGCLH+SVLSGADESH P V+  A
Sbjct: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFAA 180

Query: 181 EDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYDRDDVVLCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYY   DV+LCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLHLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVSLL LVEKYKVSIM IVPPI LAIAKSP+ EKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLQLVEKYKVSIMPIVPPIFLAIAKSPDLEKYDVS 300

Query: 301 SVRILKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGSC 360
           SVR+LKSGGAPLGKELE+AVR KFP+A+LGQGYGMTEAGPVLTMSLAFAKEPFPVKAG+C
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKVVDPETGASLPANSTGEICIRGDQIMKGYLNDLESTKSTIDKEGWLHTGD 420
           GTVVRNAEMK+VDPETGASLPANS GEICIRGDQIMKGYLNDLESTK T+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKADEQAGEVPV 480
           IGFVDD++ELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIG  DE+AGEVPV
Sbjct: 421 IGFVDDEEELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEEAGEVPV 480

Query: 481 AFVVKAEGDAIAEEDVKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKA G AI EEDV QFIS+QVVFYKRL RVFFVN IPKAPSGKILRKELRAKL +GA
Sbjct: 481 AFVVKANGGAITEEDVSQFISRQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLGNGA 540

Query: 541 YH 543
           Y+
Sbjct: 541 YN 542

BLAST of Lcy05g003790 vs. ExPASy TrEMBL
Match: A0A6J1KVH9 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111497965 PE=4 SV=1)

HSP 1 Score: 947.6 bits (2448), Expect = 2.3e-272
Identity = 476/542 (87.82%), Postives = 503/542 (92.80%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATGHVYSYDDVQ 60
           MA E NQT EFIFRSKLPDIHIPNHLPLHEY+FQN   +GSRPCLIN ATGHVYSYDDVQ
Sbjct: 1   MAAEINQTKEFIFRSKLPDIHIPNHLPLHEYIFQNLPNYGSRPCLINGATGHVYSYDDVQ 60

Query: 61  LTSRRVASGLHTLGIKKGDVVMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           LT+RRVASGLH LGIKKGDVVMNLLPNSPEFVFTFL ASYRGAIMTAANPF TAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAAYAKLIVTMACFYDKVKDLAEDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDA 180
           AKAA AKLIVTMACFYD+VKDLAE+GVKIVCVD AVEGCLH+SVLSGADESH P V+  A
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDYAVEGCLHYSVLSGADESHVPFVDFTA 180

Query: 181 EDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYDRDDVVLCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYY R+DV+LCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYKRNDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLHLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVSLL LVEKYK SI+ IVPPILLAIAKS EFEKYD+S
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELVEKYKASIVPIVPPILLAIAKSSEFEKYDMS 300

Query: 301 SVRILKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGSC 360
           SVR+LKSGGAPLGK+LE+AVR KFP+A+LGQGYGMTEAGPVLTMSLAFAKEPFPVK G+C
Sbjct: 301 SVRVLKSGGAPLGKKLEDAVREKFPTAILGQGYGMTEAGPVLTMSLAFAKEPFPVKPGAC 360

Query: 361 GTVVRNAEMKVVDPETGASLPANSTGEICIRGDQIMKGYLNDLESTKSTIDKEGWLHTGD 420
           GTVVRNAEMK++DPETG SLPANS GEICIRGDQIMKGYLNDLESTK TIDKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIIDPETGVSLPANSAGEICIRGDQIMKGYLNDLESTKRTIDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKADEQAGEVPV 480
           IGF+DDDDELFIVDRLKELIKFK FQVAPAELEALLI HPKLSDAAVIG  DEQAGE+PV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKTFQVAPAELEALLIAHPKLSDAAVIGMPDEQAGELPV 480

Query: 481 AFVVKAEGDAIAEEDVKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVV A G  I EEDVKQFIS+QVVFYKRL RVFFVN IPKAPSGKILRKELR++LA+G 
Sbjct: 481 AFVVNANGGEITEEDVKQFISEQVVFYKRLKRVFFVNAIPKAPSGKILRKELRSQLAAGV 540

Query: 541 YH 543
           Y+
Sbjct: 541 YN 542

BLAST of Lcy05g003790 vs. NCBI nr
Match: XP_023547628.1 (4-coumarate--CoA ligase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 961.4 bits (2484), Expect = 3.2e-276
Identity = 487/542 (89.85%), Postives = 511/542 (94.28%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATGHVYSYDDVQ 60
           MAVE+NQT+EFIFRSKLPDIHIPNHLPLHEYVFQN +KFGSRPCLIN ATG V+SY DVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDVHSYHDVQ 60

Query: 61  LTSRRVASGLHTLGIKKGDVVMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           L SRRVA+GLH LGIKKGDVVMNLLPNSPEFVFTFLAASY GA+MTAANPF TAVEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLAASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAAYAKLIVTMACFYDKVKDLAEDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDA 180
           AKAA AKLIVTMACFYD+VKDLAE+GVKIVCVD AVEGCLH+SVLSGADESH P VE  A
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPAVEFAA 180

Query: 181 EDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYDRDDVVLCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYY   DV+LCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLHLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVSLL L+EK+KVSIM IVPPI LAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRILKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGSC 360
           SVR+LKSGGAPLGKELE+AVR KFP+AVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAG+C
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKVVDPETGASLPANSTGEICIRGDQIMKGYLNDLESTKSTIDKEGWLHTGD 420
           GTVVRNAEMK+VDPETGASLPANS GEICIRG+QIMKGYLNDLESTK T+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKADEQAGEVPV 480
           IGF+DDDDELFIVDRLKELIKFKAFQVAPAELE+LLITHPKLSDAAVIG  DEQAGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKAEGDAIAEEDVKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKA G AI EEDVKQFISKQVVFYKRL RVFFVN IPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YH 543
           ++
Sbjct: 541 FN 542

BLAST of Lcy05g003790 vs. NCBI nr
Match: XP_022991872.1 (4-coumarate--CoA ligase 2-like [Cucurbita maxima])

HSP 1 Score: 961.4 bits (2484), Expect = 3.2e-276
Identity = 486/542 (89.67%), Postives = 510/542 (94.10%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATGHVYSYDDVQ 60
           MAVE+NQT+EFIFRSKLPDIHIPNHLPLHEYVFQN +KFGSRPCLIN ATG +YSY DVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDIYSYHDVQ 60

Query: 61  LTSRRVASGLHTLGIKKGDVVMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           L SRRVA+GLH LGIKKGDVVMNLLPNSPEFVFTFL ASY GA+MTAANPF TAVEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAAYAKLIVTMACFYDKVKDLAEDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDA 180
           AKAA AKLIVTMACFYD+VKDLAE+GVKIVCVD AVEGCLH+SVLSGADESH PPVE  A
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPPVEFAA 180

Query: 181 EDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYDRDDVVLCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYY   DV+LCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLHLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVSLL L+EK+KVSIM IVPPI LAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRILKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGSC 360
           SVR+LKSGGAPLGKELE+AVR KFP+AVLGQGYGMTEAGPVLTMSLAFAKEPF VKAG+C
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFAVKAGAC 360

Query: 361 GTVVRNAEMKVVDPETGASLPANSTGEICIRGDQIMKGYLNDLESTKSTIDKEGWLHTGD 420
           GTVVRNAEMK+VDPETGASLPANS GEICIRG+QIMKGYLNDLESTK T+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKADEQAGEVPV 480
           IGF+DDDDELFIVDRLKELIKFKAFQVAPAELE+LLITHPKLSDAAVIG  DEQAGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKAEGDAIAEEDVKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKA G AI EEDVKQFISKQVVFYKRL RVFFVN IPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YH 543
           ++
Sbjct: 541 FN 542

BLAST of Lcy05g003790 vs. NCBI nr
Match: XP_022953208.1 (4-coumarate--CoA ligase 2-like [Cucurbita moschata])

HSP 1 Score: 960.3 bits (2481), Expect = 7.1e-276
Identity = 486/542 (89.67%), Postives = 510/542 (94.10%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATGHVYSYDDVQ 60
           MAVE+NQT+EFIFRSKLPDIHIPNHLPLHEYVFQN +KFGSRPCLIN ATG VYSY DVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVASGLHTLGIKKGDVVMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           L SRRVA+GLH LGIKK DVVMNLLPNSPEFVFTFLAASY GA+MTAANPF TAVEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKSDVVMNLLPNSPEFVFTFLAASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAAYAKLIVTMACFYDKVKDLAEDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDA 180
           AKAA AKLIVTMACFYD+VKDLAE+GVKIVCVD AVEGCLH+SVLSGADESH P VE  A
Sbjct: 121 AKAANAKLIVTMACFYDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPAVEFAA 180

Query: 181 EDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYDRDDVVLCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYY   DV+LCVLP FH+YS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGSDVILCVLPFFHVYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLHLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEI+SLL L+EK+KVSIM IVPPI LAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEILSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRILKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGSC 360
           SVR+LKSGGAPLGKELE+AVR KFP+AVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAG+C
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKVVDPETGASLPANSTGEICIRGDQIMKGYLNDLESTKSTIDKEGWLHTGD 420
           GTVVRNAEMK+VDPETGASLPANS GEICIRG+QIMKGYLNDLESTK T+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKADEQAGEVPV 480
           IGFVDDDDELFIVDRLKELIKFKAFQVAPAELE+LLITHPKLSDAAVIG  DEQAGEVPV
Sbjct: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKAEGDAIAEEDVKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKA G AI EEDVKQFISKQVVFYKRL RVFFVN IPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YH 543
           ++
Sbjct: 541 FN 542

BLAST of Lcy05g003790 vs. NCBI nr
Match: KAG6593348.1 (4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 958.0 bits (2475), Expect = 3.5e-275
Identity = 486/541 (89.83%), Postives = 504/541 (93.16%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATGHVYSYDDVQ 60
           MA E NQTNEFIFRSKLPDIHIPNHLPLH YVFQN +KFGSRPCLIN ATG VYSY DVQ
Sbjct: 1   MAAELNQTNEFIFRSKLPDIHIPNHLPLHHYVFQNQSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVASGLHTLGIKKGDVVMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           LT+RRVASGLH LGIKKGDVVMNLLPNSPEFVFTFL ASYRGAIMTAANPF TAVEIAKQ
Sbjct: 61  LTARRVASGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYRGAIMTAANPFYTAVEIAKQ 120

Query: 121 AKAAYAKLIVTMACFYDKVKDLAEDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDA 180
           AKAA  KLIVTMACFYD+VKDLAE+GVKIVCVD AVE CLH+SVLSGADESH P V+  A
Sbjct: 121 AKAANVKLIVTMACFYDRVKDLAENGVKIVCVDYAVEDCLHYSVLSGADESHLPFVDFAA 180

Query: 181 EDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYDRDDVVLCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDG+NPNLYY   DV+LCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGENPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLHLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVS L LVEKYKVSIM IVPPI LAIAKSPE EKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSFLQLVEKYKVSIMPIVPPIFLAIAKSPELEKYDVS 300

Query: 301 SVRILKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGSC 360
           SVR+LKSGGAPLGKELE+AVR KFP+A+LGQGYGMTEAGPVLTMSLAFAKEPFPVKAG+C
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPAAILGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKVVDPETGASLPANSTGEICIRGDQIMKGYLNDLESTKSTIDKEGWLHTGD 420
           GTVVRNAEMK+VDPETGASLPANS GEICIRGDQIMKGYLNDLESTK T+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPANSAGEICIRGDQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKADEQAGEVPV 480
           IGF+DDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIG  DEQAGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKAEGDAIAEEDVKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKA G AI EEDVKQFISKQVVFYKRL RVFFVN IPKAPSGKILRKELRAKLA+GA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKRVFFVNAIPKAPSGKILRKELRAKLANGA 540

Query: 541 Y 542
           Y
Sbjct: 541 Y 541

BLAST of Lcy05g003790 vs. NCBI nr
Match: KAG6575648.1 (4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 954.9 bits (2467), Expect = 3.0e-274
Identity = 484/542 (89.30%), Postives = 507/542 (93.54%), Query Frame = 0

Query: 1   MAVETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATGHVYSYDDVQ 60
           MAVE+NQT+EFIFRSKLPDIHIPNHLPLHEYVFQN +KFGSRPCLIN ATG VYSY DVQ
Sbjct: 1   MAVESNQTHEFIFRSKLPDIHIPNHLPLHEYVFQNMSKFGSRPCLINGATGDVYSYHDVQ 60

Query: 61  LTSRRVASGLHTLGIKKGDVVMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQ 120
           L SRRVA+GLH LGIKKGDVVMNLLPNSPEFVFTFL ASY GA+MTAANPF TAVEIAKQ
Sbjct: 61  LMSRRVAAGLHNLGIKKGDVVMNLLPNSPEFVFTFLGASYCGAVMTAANPFYTAVEIAKQ 120

Query: 121 AKAAYAKLIVTMACFYDKVKDLAEDGVKIVCVDLAVEGCLHFSVLSGADESHAPPVEIDA 180
           AKAA AKLIVTMACF D+VKDLAE+GVKIVCVD AVEGCLH+SVLSGADESH P VE  A
Sbjct: 121 AKAANAKLIVTMACFNDRVKDLAENGVKIVCVDFAVEGCLHYSVLSGADESHVPAVEFAA 180

Query: 181 EDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYDRDDVVLCVLPLFHIYS 240
           +DVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYY   DV+LCVLP FHIYS
Sbjct: 181 DDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYHGGDVILCVLPFFHIYS 240

Query: 241 LNSILLCGLRAGAAILIMQKFEIVSLLHLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVS 300
           LNSILLCGLRAGAAILIMQKFEIVSLL L+EK+KVSIM IVPPI LAIAKSPEFEKYDVS
Sbjct: 241 LNSILLCGLRAGAAILIMQKFEIVSLLELIEKHKVSIMPIVPPIFLAIAKSPEFEKYDVS 300

Query: 301 SVRILKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGSC 360
           SVR+LKSGGAPLGKELE+AVR KFP+AVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAG+C
Sbjct: 301 SVRVLKSGGAPLGKELEDAVREKFPTAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGAC 360

Query: 361 GTVVRNAEMKVVDPETGASLPANSTGEICIRGDQIMKGYLNDLESTKSTIDKEGWLHTGD 420
           GTVVRNAEMK+VDPETGASLP NS GEICIRG+QIMKGYLNDLESTK T+DKEGWLHTGD
Sbjct: 361 GTVVRNAEMKIVDPETGASLPTNSAGEICIRGEQIMKGYLNDLESTKRTVDKEGWLHTGD 420

Query: 421 IGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKADEQAGEVPV 480
           IGF+DDDDELFIVDRLKELIKFKAFQVAPAELE+LLITHPKLSDAAVIG  DEQAGEVPV
Sbjct: 421 IGFIDDDDELFIVDRLKELIKFKAFQVAPAELESLLITHPKLSDAAVIGMPDEQAGEVPV 480

Query: 481 AFVVKAEGDAIAEEDVKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASGA 540
           AFVVKA G AI EEDVKQFISKQVVFYKRL  VFFVN IPKAPSGKILRKELRAKLASGA
Sbjct: 481 AFVVKANGGAITEEDVKQFISKQVVFYKRLKHVFFVNAIPKAPSGKILRKELRAKLASGA 540

Query: 541 YH 543
           ++
Sbjct: 541 FN 542

BLAST of Lcy05g003790 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 757.7 bits (1955), Expect = 6.5e-219
Identity = 371/536 (69.22%), Postives = 457/536 (85.26%), Query Frame = 0

Query: 8   TNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATGHVYSYDDVQLTSRRVA 67
           +N+ IFRS+LPDI+IPNHLPLH+Y+F+N ++F ++PCLIN  TG VY+Y DV +TSR++A
Sbjct: 18  SNDVIFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 77

Query: 68  SGLHTLGIKKGDVVMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQAKAAYAK 127
           +GLH LG+K+ DVVM LLPNSPE V TFLAAS+ GAI T+ANPF T  EI+KQAKA+ AK
Sbjct: 78  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 137

Query: 128 LIVTMACFYDKVKDLAEDGVKIVCV--DLAVEGCLHFSVLSGADESHAP--PVEIDAEDV 187
           LIVT + + DK+K+L  DGV IV    D   E CL FS L+ ++E      P +I  EDV
Sbjct: 138 LIVTQSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPEDV 197

Query: 188 VALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYDRDDVVLCVLPLFHIYSLNS 247
           VALP+SSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY++RDDV+LCVLP+FHIY+LNS
Sbjct: 198 VALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNS 257

Query: 248 ILLCGLRAGAAILIMQKFEIVSLLHLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVSSVR 307
           I+LC LR GA ILIM KFEI  LL  +++ KV++ ++VPPI+LAIAKSPE EKYD+SSVR
Sbjct: 258 IMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVR 317

Query: 308 ILKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGSCGTV 367
           ++KSG APLGKELE+A+ AKFP+A LGQGYGMTEAGPVL MSL FAKEPFPVK+G+CGTV
Sbjct: 318 MVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTV 377

Query: 368 VRNAEMKVVDPETGASLPANSTGEICIRGDQIMKGYLNDLESTKSTIDKEGWLHTGDIGF 427
           VRNAEMK++DP+TG SLP N  GEICIRG+QIMKGYLND  +T STIDK+GWLHTGD+GF
Sbjct: 378 VRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGF 437

Query: 428 VDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKADEQAGEVPVAFV 487
           +DDDDELFIVDRLKELIK+K FQVAPAELE+LLI HP+++D AV+   +E AGEVPVAFV
Sbjct: 438 IDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFV 497

Query: 488 VKAEGDAIAEEDVKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKLASG 540
           V+++   I+E+++KQF+SKQVVFYKR+N+VFF ++IPKAPSGKILRK+LRA+LA+G
Sbjct: 498 VRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANG 553

BLAST of Lcy05g003790 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 745.0 bits (1922), Expect = 4.4e-215
Identity = 373/543 (68.69%), Postives = 447/543 (82.32%), Query Frame = 0

Query: 6   NQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATGHVYSYDDVQLTSRR 65
           N  ++ IFRSKLPDI+IPNHL LH+Y+FQN ++F ++PCLIN  TGHVY+Y DV + SR+
Sbjct: 18  NNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQ 77

Query: 66  VASGLHTLGIKKGDVVMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQAKAAY 125
           +A+  H LG+ + DVVM LLPN PEFV +FLAAS+RGA  TAANPF T  EIAKQAKA+ 
Sbjct: 78  IAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASN 137

Query: 126 AKLIVTMACFYDKVKDLA-EDGVKIVCVD-----LAVEGCLHFSVLSGADESHA---PPV 185
            KLI+T A + DK+K L  +DGV IVC+D        EGCL F+ L+ +    +     V
Sbjct: 138 TKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSV 197

Query: 186 EIDAEDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYDRDDVVLCVLPLF 245
           EI  +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY+  DDV+LCVLP+F
Sbjct: 198 EISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMF 257

Query: 246 HIYSLNSILLCGLRAGAAILIMQKFEIVSLLHLVEKYKVSIMLIVPPILLAIAKSPEFEK 305
           HIY+LNSI+LCGLR GAAILIM KFEI  LL L+++ KV++  +VPPI+LAIAKS E EK
Sbjct: 258 HIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEK 317

Query: 306 YDVSSVRILKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLAFAKEPFPVK 365
           YD+SS+R++KSG APLGKELE+AV AKFP+A LGQGYGMTEAGPVL MSL FAKEPFPVK
Sbjct: 318 YDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVK 377

Query: 366 AGSCGTVVRNAEMKVVDPETGASLPANSTGEICIRGDQIMKGYLNDLESTKSTIDKEGWL 425
           +G+CGTVVRNAEMK+VDP+TG SL  N  GEICIRG QIMKGYLN+  +T  TIDK+GWL
Sbjct: 378 SGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWL 437

Query: 426 HTGDIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKADEQAG 485
           HTGDIG +DDDDELFIVDRLKELIK+K FQVAPAELEALLI HP ++D AV+   +E AG
Sbjct: 438 HTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAG 497

Query: 486 EVPVAFVVKAEGDAIAEEDVKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKL 540
           EVPVAFVVK++   ++E+DVKQF+SKQVVFYKR+N+VFF  +IPKAPSGKILRK+LRAKL
Sbjct: 498 EVPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKL 557

BLAST of Lcy05g003790 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 687.6 bits (1773), Expect = 8.3e-198
Identity = 345/508 (67.91%), Postives = 414/508 (81.50%), Query Frame = 0

Query: 6   NQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATGHVYSYDDVQLTSRR 65
           N  ++ IFRSKLPDI+IPNHL LH+Y+FQN ++F ++PCLIN  TGHVY+Y DV + SR+
Sbjct: 18  NNNSDVIFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQ 77

Query: 66  VASGLHTLGIKKGDVVMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQAKAAY 125
           +A+  H LG+ + DVVM LLPN PEFV +FLAAS+RGA  TAANPF T  EIAKQAKA+ 
Sbjct: 78  IAANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASN 137

Query: 126 AKLIVTMACFYDKVKDLA-EDGVKIVCVD-----LAVEGCLHFSVLSGADESHA---PPV 185
            KLI+T A + DK+K L  +DGV IVC+D        EGCL F+ L+ +    +     V
Sbjct: 138 TKLIITEARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSV 197

Query: 186 EIDAEDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYDRDDVVLCVLPLF 245
           EI  +DVVALPYSSGTTGLPKGVMLTHKGL+TSVAQQ+DG+NPNLY+  DDV+LCVLP+F
Sbjct: 198 EISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMF 257

Query: 246 HIYSLNSILLCGLRAGAAILIMQKFEIVSLLHLVEKYKVSIMLIVPPILLAIAKSPEFEK 305
           HIY+LNSI+LCGLR GAAILIM KFEI  LL L+++ KV++  +VPPI+LAIAKS E EK
Sbjct: 258 HIYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEK 317

Query: 306 YDVSSVRILKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLAFAKEPFPVK 365
           YD+SS+R++KSG APLGKELE+AV AKFP+A LGQGYGMTEAGPVL MSL FAKEPFPVK
Sbjct: 318 YDLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVK 377

Query: 366 AGSCGTVVRNAEMKVVDPETGASLPANSTGEICIRGDQIMKGYLNDLESTKSTIDKEGWL 425
           +G+CGTVVRNAEMK+VDP+TG SL  N  GEICIRG QIMKGYLN+  +T  TIDK+GWL
Sbjct: 378 SGACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWL 437

Query: 426 HTGDIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKADEQAG 485
           HTGDIG +DDDDELFIVDRLKELIK+K FQVAPAELEALLI HP ++D AV+   +E AG
Sbjct: 438 HTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAG 497

Query: 486 EVPVAFVVKAEGDAIAEEDVKQFISKQV 505
           EVPVAFVVK++   ++E+DVKQF+SKQV
Sbjct: 498 EVPVAFVVKSKDSELSEDDVKQFVSKQV 525

BLAST of Lcy05g003790 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 672.9 bits (1735), Expect = 2.1e-193
Identity = 333/546 (60.99%), Postives = 425/546 (77.84%), Query Frame = 0

Query: 4   ETNQTNEFIFRSKLPDIHIPNHLPLHEYVFQNNAKFG----SRPCLINAATGHVYSYDDV 63
           E   +++FIFRSKLPDI IPNHLPL +YVFQ  +  G    S  C+I+ ATG + +Y DV
Sbjct: 20  EEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADV 79

Query: 64  QLTSRRVASGLHTLGIKKGDVVMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAK 123
           Q   RR+A+G+H LGI+ GDVVM LLPNSPEF  +FLA +Y GA+ T ANPF T  EIAK
Sbjct: 80  QTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAK 139

Query: 124 QAKAAYAKLIVTMACFYDKVKDLAEDGVKIVCVD---------LAVEGCLHFSVLSGADE 183
           QAKA+ AK+I+T  C  DK+ +L  DGV IVC+D          + +GC+ F+ L+ ADE
Sbjct: 140 QAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADE 199

Query: 184 SHAPPVEIDAEDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYDRDDVVL 243
           +     +I  ED VA+PYSSGTTGLPKGVM+THKGL+TS+AQ++DG+NPNL +  +DV+L
Sbjct: 200 TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVIL 259

Query: 244 CVLPLFHIYSLNSILLCGLRAGAAILIMQKFEIVSLLHLVEKYKVSIMLIVPPILLAIAK 303
           C LP+FHIY+L++++L  +R GAA+LI+ +FE+  ++ L+++YKV+++ + PP++LA  K
Sbjct: 260 CFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIK 319

Query: 304 SPEFEKYDVSSVRILKSGGAPLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLAFAK 363
           SPE E+YD+SSVRI+ SG A L KELE+AVR KFP+A+ GQGYGMTE+G V   SLAFAK
Sbjct: 320 SPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAK 379

Query: 364 EPFPVKAGSCGTVVRNAEMKVVDPETGASLPANSTGEICIRGDQIMKGYLNDLESTKSTI 423
            PF  K+G+CGTV+RNAEMKVVD ETG SLP N +GEIC+RG Q+MKGYLND E+T  TI
Sbjct: 380 NPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTI 439

Query: 424 DKEGWLHTGDIGFVDDDDELFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGK 483
           DK+GWLHTGDIGFVDDDDE+FIVDRLKELIKFK +QVAPAELEALLI+HP + DAAV+  
Sbjct: 440 DKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAM 499

Query: 484 ADEQAGEVPVAFVVKAEGDAIAEEDVKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRK 537
            DE A EVPVAFV +++G  + E+DVK +++KQVV YKR+  VFF+  IPKA SGKILRK
Sbjct: 500 KDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRK 559

BLAST of Lcy05g003790 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 660.6 bits (1703), Expect = 1.1e-189
Identity = 332/527 (63.00%), Postives = 417/527 (79.13%), Query Frame = 0

Query: 12  IFRSKLPDIHIPNHLPLHEYVFQNNAKFGSRPCLINAATGHVYSYDDVQLTSRRVASGLH 71
           IFRSKLPDI IPNHLPLH Y F+  +    +PCLI  +TG  Y+Y +  L  RRVASGL+
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 72  TLGIKKGDVVMNLLPNSPEFVFTFLAASYRGAIMTAANPFCTAVEIAKQAKAAYAKLIVT 131
            LGI+KGDV+M LL NS EFVF+F+ AS  GA+ T ANPF T+ E+ KQ K++ AKLI+T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 132 MACFYDKVKDLAEDGVKIVCVDLAVEGCLHFSVLSGADESH--APPVEIDAEDVVALPYS 191
            + + DK+K+L E+   I   +   E CL FS L   DE++     V+I  +D  ALP+S
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAALPFS 213

Query: 192 SGTTGLPKGVMLTHKGLITSVAQQIDGQNPNLYYDRDDVVLCVLPLFHIYSLNSILLCGL 251
           SGTTGLPKGV+LTHK LITSVAQQ+DG NPNLY   +DV+LCVLPLFHIYSLNS+LL  L
Sbjct: 214 SGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNSL 273

Query: 252 RAGAAILIMQKFEIVSLLHLVEKYKVSIMLIVPPILLAIAKSPEFEKYDVSSVRILKSGG 311
           R+GA +L+M KFEI +LL L+++++V+I  +VPP+++A+AK+P    YD+SSVR + SG 
Sbjct: 274 RSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFVLSGA 333

Query: 312 APLGKELENAVRAKFPSAVLGQGYGMTEAGPVLTMSLAFAKEPFPVKAGSCGTVVRNAEM 371
           APLGKEL++++R + P A+LGQGYGMTEAGPVL+MSL FAKEP P K+GSCGTVVRNAE+
Sbjct: 334 APLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNAEL 393

Query: 372 KVVDPETGASLPANSTGEICIRGDQIMKGYLNDLESTKSTIDKEGWLHTGDIGFVDDDDE 431
           KVV  ET  SL  N  GEICIRG QIMK YLND E+T +TID+EGWLHTGDIG+VD+DDE
Sbjct: 394 KVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVDEDDE 453

Query: 432 LFIVDRLKELIKFKAFQVAPAELEALLITHPKLSDAAVIGKADEQAGEVPVAFVVKAEGD 491
           +FIVDRLKE+IKFK FQV PAELE+LLI H  ++DAAV+ + DE AGEVPVAFVV++ G+
Sbjct: 454 IFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNGN 513

Query: 492 AIAEEDVKQFISKQVVFYKRLNRVFFVNTIPKAPSGKILRKELRAKL 537
            I EEDVK++++KQVVFYKRL++VFFV +IPK+PSGKILRK+L+AKL
Sbjct: 514 DITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560