Lcy05g003590 (gene) Sponge gourd (P93075) v1

Overview
NameLcy05g003590
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionHeat shock 70 kDa protein
LocationChr05: 3199911 .. 3202734 (-)
RNA-Seq ExpressionLcy05g003590
SyntenyLcy05g003590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACCTTTATACCAAAAGATCAGAAGTTCGAATCTCGAACTCCAAAGCTTGTTCAAGGTTAAAAAACAACATTTTCCTCTAATTTCTTAACCTCATACAAATTAATTTTTTTTATTAAAATATTTTTATTTCAAGTCTAAATTTCCCTACTATTTTCCACATGCGCAATCAATCCAACTCTGCCGAGTCATCCGAGCCAACTCACCCACTCTCCAGAAATCTCCATCTTCTTCCAGATTCCTCTGCAACTCTTTTCCCGCCATTCCTTCTACCACCTTCTCCATGAAAACCTCTCCGCTTAGTTCTCCATATAAAAGCAACCCTCATCCTCCCACTTCCCTCCTCAGATCAAAACTATCAAGACTTCCATTCAGCATTCTACATTTCCGATCCCGATTTCTTCATCAATTTCCATTTTCAACATCTTCCAGTTCTAGTCAAGCTTTCATCAATGGCTTCCAAGAGCGAAGGCAAAGGCATTGGCATTGATCTCGGCACCACTTACAGCTGCGTCGGCGTTTGGCAAAACGATAGGGTTGAGATTATTGCCAACGACCAAGGCAACAGAACTACGCCCTCCTATGTGGCTTTCACCGATACCGAACGGCTTATCGGCGATGCGGCCAAGAATCAGGTCGCGATGAATCCTCAGAACACCGTTTTTGACGCCAAGCGGCTCATCGGCCGCCGATTCTCCGACTCTTCCGTTCAGAGCGATATGAAGCTTTGGCCATTCAAGGTCGTTGCAGGTAAGCACATGCTCTAATCGATTCATTTTGGTTGAAATAGACATATTCGGTTCCTCTGCTTTCGTTAACAATTTTGTTTGGAAGCTCTAGTTGGTACATGTGAATTGGACTAAATCGCTACAATACTAAATCATTACATGCTTAATTGAAGTACTGTGTTGTTCATTCAAATGAATCAAATGTGCTTTTTATCCATGTGATTTTCTATCCTGATGTAGTTTTGGAGAAGACTGAATTCTTACCTTGATCTTTTCAGGTCCTGGAGACAAGCCGATGATCGTCGTCAACTACAAAGGCGAAGAGAAACAATTCGCGCCAGAGGAGATTTCGTCAATGGTGCTGATCAAGATGAAGGAAATCGCCGAGGCTTACTTAGGGCATAATGTAAAGAACGCTGTGATTACAGTCCCCGCCTATTTCAACGATTCACAGCGGCAGGCCACCAAGGACGCCGGAGCAATCGCTGGCCTAAACGTACTGAGAATCATCAACGAGCCAACTGCCGCCGCCATTGCTTACGGTCTGGACAAGAAAGCCTCCCGCTCCGGCGAGAAGAACGTTCTGATCTTCGATCTCGGCGGCGGAACGTTCGACGTTTCGCTGTTGACGATCGAGGAAGGAATCTTCGAAGTGAAAGCCACCGCCGGAGACACTCATCTCGGCGGCGAGGATTTCGATAACCGCCTCGTGAACCACTTCGCGGCGGAGTTCAAAAGGAAGCACAAGAAGGACATCGGCGGCAATGCCAGAGCTCTCCGTCGGCTGAGAACGGCCTGCGAAAGGGCGAAACGGACTCTATCTTCGACAACACAAACTACGATCGAAATCGATTCTTTGTACGAAGGAATTGACTTCTACGCCACGATTACCAGGGCGAGATTCGAGGAACTGAACATGGATTTGTTCAGAAGGTGTATGGAACCAGTGGAGAAATGTCTGCGAGATTCCAAAATTGACAAGGGGCAAATACACGACGTCGTTTTGGTTGGCGGTTCCACAAGAATCCCTAAAGTTCAGCAGCTATTGCAAGATTTCTTCAACGGAAAAGAGCTCTGCAAGAGCATAAACCCTGACGAAGCCGTCGCGTATGGCGCCGCCGTCCAGGCGGCGATTCTGTGCGGCGAAGGCAACGAGAAGGTCCAAGATCTACTTCTTCTCGACGTCACACCTCTAAGTCTTGGAATCGAAACCGCCGGCGGAGTAATGACGGTGTTAATTCCAAGAAACACGACGATTCCGACGAAGAAGGAGCAAGTTTTCTCGACTTATTCGGACAATCAAACGAGCGTTCTAATCCAAGTTTATGAAGGGGAAAGAGCAAGAACAAAAGACAATAACCTTCTAGGAACATTTGAGCTCAAGGGGATTCCACCGGCGCCGAGAGGAGTGCCGCAAATCAACGTCTGTTTCGACATCGACGCCAATGGGATTCTGAACGTCACGGCGGAGGACAAGACGGCCGGAGTTAAGAACACGATCACCATTACCAACGACAAGGGGAGATTGAGCAGAGATGAGATTGAGAGGATGGTGCAGGAGGCGGAGAAGTACAAGGCGGAGGATGAACAGGTAAAGAAGAAGGTTGAGGCGAAGAACGCCTTGGAGAATTATGCTTATAACATGAGGAACACCGTGAGGGATGAGAAATTGGCCGGAAAGATGAATCCGGCGGAGAAGGGGAAGATAGAGAAGGCGATTGATGAGGCAATTGATTGGCTGGAGAGGAACCAGTTGGCTGAAGTGGATGAATTTGAGGACAAGCTCAAGGAACTAGAGGGGCTTTGTAATCCTATCATTGCTAAGCTTTATCAGGGTGGTGCTGGTGGAGATATGCCGATGGGTAACGGCGGTGGTGCCGGCTGCAGCGGCGGCGCTGGAGCTGGGCCAAAGATTGAAGAGGTCGATTGAGGTCTGAAGACTCTGAACCTTCGAACTATATCTAATACTGTCCTTGTTCTTTTTGTGTTGTGTTCTGTAAAACTTTTACGTTTGAGTTTGTATCTTTTTCGAAGAACAAGCTTCTTTTATGTACAAATTTTTTAAAACAATAAATGTTTCAGAATAAGGGGAAATTTGC

mRNA sequence

ACCTTTATACCAAAAGATCAGAAGTTCGAATCTCGAACTCCAAAGCTTGTTCAAGGTTAAAAAACAACATTTTCCTCTAATTTCTTAACCTCATACAAATTAATTTTTTTTATTAAAATATTTTTATTTCAAGTCTAAATTTCCCTACTATTTTCCACATGCGCAATCAATCCAACTCTGCCGAGTCATCCGAGCCAACTCACCCACTCTCCAGAAATCTCCATCTTCTTCCAGATTCCTCTGCAACTCTTTTCCCGCCATTCCTTCTACCACCTTCTCCATGAAAACCTCTCCGCTTAGTTCTCCATATAAAAGCAACCCTCATCCTCCCACTTCCCTCCTCAGATCAAAACTATCAAGACTTCCATTCAGCATTCTACATTTCCGATCCCGATTTCTTCATCAATTTCCATTTTCAACATCTTCCAGTTCTAGTCAAGCTTTCATCAATGGCTTCCAAGAGCGAAGGCAAAGGCATTGGCATTGATCTCGGCACCACTTACAGCTGCGTCGGCGTTTGGCAAAACGATAGGGTTGAGATTATTGCCAACGACCAAGGCAACAGAACTACGCCCTCCTATGTGGCTTTCACCGATACCGAACGGCTTATCGGCGATGCGGCCAAGAATCAGGTCGCGATGAATCCTCAGAACACCGTTTTTGACGCCAAGCGGCTCATCGGCCGCCGATTCTCCGACTCTTCCGTTCAGAGCGATATGAAGCTTTGGCCATTCAAGGTCGTTGCAGGTAAGCACATGCTCTAATCGATTCATTTTGGTTGAAATAGACATATTCGGTTCCTCTGCTTTCGTTAACAATTTTGTTTGGAAGCTCTAGTTGGTACATGTGAATTGGACTAAATCGCTACAATACTAAATCATTACATGCTTAATTGAAGTACTGTGTTGTTCATTCAAATGAATCAAATGTGCTTTTTATCCATGTGATTTTCTATCCTGATGTAGTTTTGGAGAAGACTGAATTCTTACCTTGATCTTTTCAGGTCCTGGAGACAAGCCGATGATCGTCGTCAACTACAAAGGCGAAGAGAAACAATTCGCGCCAGAGGAGATTTCGTCAATGGTGCTGATCAAGATGAAGGAAATCGCCGAGGCTTACTTAGGGCATAATGTAAAGAACGCTGTGATTACAGTCCCCGCCTATTTCAACGATTCACAGCGGCAGGCCACCAAGGACGCCGGAGCAATCGCTGGCCTAAACGTACTGAGAATCATCAACGAGCCAACTGCCGCCGCCATTGCTTACGGTCTGGACAAGAAAGCCTCCCGCTCCGGCGAGAAGAACGTTCTGATCTTCGATCTCGGCGGCGGAACGTTCGACGTTTCGCTGTTGACGATCGAGGAAGGAATCTTCGAAGTGAAAGCCACCGCCGGAGACACTCATCTCGGCGGCGAGGATTTCGATAACCGCCTCGTGAACCACTTCGCGGCGGAGTTCAAAAGGAAGCACAAGAAGGACATCGGCGGCAATGCCAGAGCTCTCCGTCGGCTGAGAACGGCCTGCGAAAGGGCGAAACGGACTCTATCTTCGACAACACAAACTACGATCGAAATCGATTCTTTGTACGAAGGAATTGACTTCTACGCCACGATTACCAGGGCGAGATTCGAGGAACTGAACATGGATTTGTTCAGAAGGTGTATGGAACCAGTGGAGAAATGTCTGCGAGATTCCAAAATTGACAAGGGGCAAATACACGACGTCGTTTTGGTTGGCGGTTCCACAAGAATCCCTAAAGTTCAGCAGCTATTGCAAGATTTCTTCAACGGAAAAGAGCTCTGCAAGAGCATAAACCCTGACGAAGCCGTCGCGTATGGCGCCGCCGTCCAGGCGGCGATTCTGTGCGGCGAAGGCAACGAGAAGGTCCAAGATCTACTTCTTCTCGACGTCACACCTCTAAGTCTTGGAATCGAAACCGCCGGCGGAGTAATGACGGTGTTAATTCCAAGAAACACGACGATTCCGACGAAGAAGGAGCAAGTTTTCTCGACTTATTCGGACAATCAAACGAGCGTTCTAATCCAAGTTTATGAAGGGGAAAGAGCAAGAACAAAAGACAATAACCTTCTAGGAACATTTGAGCTCAAGGGGATTCCACCGGCGCCGAGAGGAGTGCCGCAAATCAACGTCTGTTTCGACATCGACGCCAATGGGATTCTGAACGTCACGGCGGAGGACAAGACGGCCGGAGTTAAGAACACGATCACCATTACCAACGACAAGGGGAGATTGAGCAGAGATGAGATTGAGAGGATGGTGCAGGAGGCGGAGAAGTACAAGGCGGAGGATGAACAGGTAAAGAAGAAGGTTGAGGCGAAGAACGCCTTGGAGAATTATGCTTATAACATGAGGAACACCGTGAGGGATGAGAAATTGGCCGGAAAGATGAATCCGGCGGAGAAGGGGAAGATAGAGAAGGCGATTGATGAGGCAATTGATTGGCTGGAGAGGAACCAGTTGGCTGAAGTGGATGAATTTGAGGACAAGCTCAAGGAACTAGAGGGGCTTTGTAATCCTATCATTGCTAAGCTTTATCAGGGTGGTGCTGGTGGAGATATGCCGATGGGTAACGGCGGTGGTGCCGGCTGCAGCGGCGGCGCTGGAGCTGGGCCAAAGATTGAAGAGGTCGATTGAGGTCTGAAGACTCTGAACCTTCGAACTATATCTAATACTGTCCTTGTTCTTTTTGTGTTGTGTTCTGTAAAACTTTTACGTTTGAGTTTGTATCTTTTTCGAAGAACAAGCTTCTTTTATGTACAAATTTTTTAAAACAATAAATGTTTCAGAATAAGGGGAAATTTGC

Coding sequence (CDS)

ATGATCGTCGTCAACTACAAAGGCGAAGAGAAACAATTCGCGCCAGAGGAGATTTCGTCAATGGTGCTGATCAAGATGAAGGAAATCGCCGAGGCTTACTTAGGGCATAATGTAAAGAACGCTGTGATTACAGTCCCCGCCTATTTCAACGATTCACAGCGGCAGGCCACCAAGGACGCCGGAGCAATCGCTGGCCTAAACGTACTGAGAATCATCAACGAGCCAACTGCCGCCGCCATTGCTTACGGTCTGGACAAGAAAGCCTCCCGCTCCGGCGAGAAGAACGTTCTGATCTTCGATCTCGGCGGCGGAACGTTCGACGTTTCGCTGTTGACGATCGAGGAAGGAATCTTCGAAGTGAAAGCCACCGCCGGAGACACTCATCTCGGCGGCGAGGATTTCGATAACCGCCTCGTGAACCACTTCGCGGCGGAGTTCAAAAGGAAGCACAAGAAGGACATCGGCGGCAATGCCAGAGCTCTCCGTCGGCTGAGAACGGCCTGCGAAAGGGCGAAACGGACTCTATCTTCGACAACACAAACTACGATCGAAATCGATTCTTTGTACGAAGGAATTGACTTCTACGCCACGATTACCAGGGCGAGATTCGAGGAACTGAACATGGATTTGTTCAGAAGGTGTATGGAACCAGTGGAGAAATGTCTGCGAGATTCCAAAATTGACAAGGGGCAAATACACGACGTCGTTTTGGTTGGCGGTTCCACAAGAATCCCTAAAGTTCAGCAGCTATTGCAAGATTTCTTCAACGGAAAAGAGCTCTGCAAGAGCATAAACCCTGACGAAGCCGTCGCGTATGGCGCCGCCGTCCAGGCGGCGATTCTGTGCGGCGAAGGCAACGAGAAGGTCCAAGATCTACTTCTTCTCGACGTCACACCTCTAAGTCTTGGAATCGAAACCGCCGGCGGAGTAATGACGGTGTTAATTCCAAGAAACACGACGATTCCGACGAAGAAGGAGCAAGTTTTCTCGACTTATTCGGACAATCAAACGAGCGTTCTAATCCAAGTTTATGAAGGGGAAAGAGCAAGAACAAAAGACAATAACCTTCTAGGAACATTTGAGCTCAAGGGGATTCCACCGGCGCCGAGAGGAGTGCCGCAAATCAACGTCTGTTTCGACATCGACGCCAATGGGATTCTGAACGTCACGGCGGAGGACAAGACGGCCGGAGTTAAGAACACGATCACCATTACCAACGACAAGGGGAGATTGAGCAGAGATGAGATTGAGAGGATGGTGCAGGAGGCGGAGAAGTACAAGGCGGAGGATGAACAGGTAAAGAAGAAGGTTGAGGCGAAGAACGCCTTGGAGAATTATGCTTATAACATGAGGAACACCGTGAGGGATGAGAAATTGGCCGGAAAGATGAATCCGGCGGAGAAGGGGAAGATAGAGAAGGCGATTGATGAGGCAATTGATTGGCTGGAGAGGAACCAGTTGGCTGAAGTGGATGAATTTGAGGACAAGCTCAAGGAACTAGAGGGGCTTTGTAATCCTATCATTGCTAAGCTTTATCAGGGTGGTGCTGGTGGAGATATGCCGATGGGTAACGGCGGTGGTGCCGGCTGCAGCGGCGGCGCTGGAGCTGGGCCAAAGATTGAAGAGGTCGATTGA

Protein sequence

MIVVNYKGEEKQFAPEEISSMVLIKMKEIAEAYLGHNVKNAVITVPAYFNDSQRQATKDAGAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEVKATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQTTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQIHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPLSLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTFELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMVQEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTVRDEKLAGKMNPAEKGKIEKAIDEAIDWLERNQLAEVDEFEDKLKELEGLCNPIIAKLYQGGAGGDMPMGNGGGAGCSGGAGAGPKIEEVD
Homology
BLAST of Lcy05g003590 vs. ExPASy Swiss-Prot
Match: P26413 (Heat shock 70 kDa protein OS=Glycine max OX=3847 GN=HSP70 PE=3 SV=1)

HSP 1 Score: 926.0 bits (2392), Expect = 1.9e-268
Identity = 469/546 (85.90%), Postives = 514/546 (94.14%), Query Frame = 0

Query: 1   MIVVNYKGEEKQFAPEEISSMVLIKMKEIAEAYLGHNVKNAVITVPAYFNDSQRQATKDA 60
           MIVVNYKGEEK+F+ EEISSMVL+KM+E+AEA+LGH VKNAV+TVPAYFNDSQRQATKDA
Sbjct: 105 MIVVNYKGEEKKFSAEEISSMVLVKMREVAEAFLGHAVKNAVVTVPAYFNDSQRQATKDA 164

Query: 61  GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 120
           GAI+GLNVLRIINEPTAAAIAYGLDKKASR GE+NVLIFDLGGGTFDVS+LTIEEGIFEV
Sbjct: 165 GAISGLNVLRIINEPTAAAIAYGLDKKASRKGEQNVLIFDLGGGTFDVSILTIEEGIFEV 224

Query: 121 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 180
           KATAGDTHLGGEDFDNR+VNHF +EFKRK+KKDI GNARALRRLRTACERAKRTLSST Q
Sbjct: 225 KATAGDTHLGGEDFDNRMVNHFVSEFKRKNKKDISGNARALRRLRTACERAKRTLSSTAQ 284

Query: 181 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQIHDVVLVGG 240
           TTIEIDSLYEGIDFYATITRARFEE+NMDLFR+CMEPVEKCLRD+KIDK Q+H+VVLVGG
Sbjct: 285 TTIEIDSLYEGIDFYATITRARFEEMNMDLFRKCMEPVEKCLRDAKIDKSQVHEVVLVGG 344

Query: 241 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL 300
           STRIPKV QLLQDFFNGKELCKSINPDEAVAYGAAVQAAIL G+G+EKVQDLLLLDVTPL
Sbjct: 345 STRIPKVHQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGQGDEKVQDLLLLDVTPL 404

Query: 301 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 360
           SLG+ETAGGVMTVLIPRNTTIPTKKEQ+FSTYSDNQ  VLIQV+EGERARTKDNNLLG F
Sbjct: 405 SLGLETAGGVMTVLIPRNTTIPTKKEQIFSTYSDNQPGVLIQVFEGERARTKDNNLLGKF 464

Query: 361 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV 420
           EL GIPPAPRGVPQ+NVCFDIDANGILNV+AEDKTAGVKN ITITNDKGRLS++EIE+MV
Sbjct: 465 ELTGIPPAPRGVPQVNVCFDIDANGILNVSAEDKTAGVKNKITITNDKGRLSKEEIEKMV 524

Query: 421 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTVRDEKLAGKMNPAEKGKIEKAIDEAID 480
           ++AE+YKAEDE+VKKKVEAKN+LENYAYNMRNT++DEK+ GK++P EK KIEKA+++AI 
Sbjct: 525 KDAERYKAEDEEVKKKVEAKNSLENYAYNMRNTIKDEKIGGKLSPDEKQKIEKAVEDAIQ 584

Query: 481 WLERNQLAEVDEFEDKLKELEGLCNPIIAKLYQG--GAGGDMPMGNGGGAGCSGGAGAGP 540
           WLE NQ+AEVDEFEDK KELEG+CNPIIAK+YQG  G GGD+PMG    A     AGAGP
Sbjct: 585 WLEGNQMAEVDEFEDKQKELEGICNPIIAKMYQGAAGPGGDVPMGADMPA-----AGAGP 644

Query: 541 KIEEVD 545
           KIEEVD
Sbjct: 645 KIEEVD 645

BLAST of Lcy05g003590 vs. ExPASy Swiss-Prot
Match: Q9S9N1 (Heat shock 70 kDa protein 5 OS=Arabidopsis thaliana OX=3702 GN=HSP70-5 PE=2 SV=1)

HSP 1 Score: 912.5 bits (2357), Expect = 2.2e-264
Identity = 465/545 (85.32%), Postives = 507/545 (93.03%), Query Frame = 0

Query: 1   MIVVNYKGEEKQFAPEEISSMVLIKMKEIAEAYLGHNVKNAVITVPAYFNDSQRQATKDA 60
           MIVV+YK EEKQF+PEEISSMVL+KMKE+AEA+LG  VKNAV+TVPAYFNDSQRQATKDA
Sbjct: 105 MIVVSYKNEEKQFSPEEISSMVLVKMKEVAEAFLGRTVKNAVVTVPAYFNDSQRQATKDA 164

Query: 61  GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 120
           GAI+GLNVLRIINEPTAAAIAYGLDKK +++GEKNVLIFDLGGGTFDVSLLTIEEG+FEV
Sbjct: 165 GAISGLNVLRIINEPTAAAIAYGLDKKGTKAGEKNVLIFDLGGGTFDVSLLTIEEGVFEV 224

Query: 121 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 180
           KATAGDTHLGGEDFDNRLVNHF AEF+RKHKKDI GNARALRRLRTACERAKRTLSST Q
Sbjct: 225 KATAGDTHLGGEDFDNRLVNHFVAEFRRKHKKDIAGNARALRRLRTACERAKRTLSSTAQ 284

Query: 181 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQIHDVVLVGG 240
           TTIEIDSL+EGIDFYATI+RARFEE+NMDLFR+CM+PVEK L+D+K+DK  +HDVVLVGG
Sbjct: 285 TTIEIDSLHEGIDFYATISRARFEEMNMDLFRKCMDPVEKVLKDAKLDKSSVHDVVLVGG 344

Query: 241 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL 300
           STRIPK+QQLLQDFFNGKELCKSINPDEAVAYGAAVQAAIL GEG+EKVQDLLLLDV PL
Sbjct: 345 STRIPKIQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILTGEGSEKVQDLLLLDVAPL 404

Query: 301 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 360
           SLG+ETAGGVMTVLIPRNTT+P KKEQVFSTY+DNQ  VLIQVYEGERART+DNNLLGTF
Sbjct: 405 SLGLETAGGVMTVLIPRNTTVPCKKEQVFSTYADNQPGVLIQVYEGERARTRDNNLLGTF 464

Query: 361 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV 420
           ELKGIPPAPRGVPQINVCFDIDANGILNV+AEDKTAGVKN ITITNDKGRLS++EIE+MV
Sbjct: 465 ELKGIPPAPRGVPQINVCFDIDANGILNVSAEDKTAGVKNQITITNDKGRLSKEEIEKMV 524

Query: 421 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTVRDEKLAGKMNPAEKGKIEKAIDEAID 480
           Q+AEKYKAEDEQVKKKVEAKN+LENYAYNMRNT++DEKLA K+   +K KIEKAIDE I+
Sbjct: 525 QDAEKYKAEDEQVKKKVEAKNSLENYAYNMRNTIKDEKLAQKLTQEDKQKIEKAIDETIE 584

Query: 481 WLERNQLAEVDEFEDKLKELEGLCNPIIAKLYQGG-AGGDMPMGNGGGAGCSGGAGAGPK 540
           W+E NQLAEVDEFE KLKELEG+CNPII+K+YQGG A G MP     G   S GA  GPK
Sbjct: 585 WIEGNQLAEVDEFEYKLKELEGICNPIISKMYQGGAAAGGMPT---DGDFSSSGAAGGPK 644

Query: 541 IEEVD 545
           IEEVD
Sbjct: 645 IEEVD 646

BLAST of Lcy05g003590 vs. ExPASy Swiss-Prot
Match: P09189 (Heat shock cognate 70 kDa protein OS=Petunia hybrida OX=4102 GN=HSP70 PE=2 SV=1)

HSP 1 Score: 910.2 bits (2351), Expect = 1.1e-263
Identity = 468/546 (85.71%), Postives = 501/546 (91.76%), Query Frame = 0

Query: 1   MIVVNYKGEEKQFAPEEISSMVLIKMKEIAEAYLGHNVKNAVITVPAYFNDSQRQATKDA 60
           MIVV YKGEEKQFA EEISSMVL KMKEIAEAYLG  +KNAV+TVPAYFNDSQRQATKDA
Sbjct: 106 MIVVTYKGEEKQFAAEEISSMVLTKMKEIAEAYLGTTIKNAVVTVPAYFNDSQRQATKDA 165

Query: 61  GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 120
           G IAGLNV+RIINEPTAAAIAYGLDKKAS +GEKNVLIFDLGGGTFDVSLLTIEEGIFEV
Sbjct: 166 GVIAGLNVMRIINEPTAAAIAYGLDKKASSAGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 225

Query: 121 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 180
           KATAGDTHLGGEDFDNR+VNHF  EFKRK+KKDI GN RALRRLRTACERAKRTLSST Q
Sbjct: 226 KATAGDTHLGGEDFDNRMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQ 285

Query: 181 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQIHDVVLVGG 240
           TTIEIDSLYEGIDFY+TITRARFEELNMDLFR+CMEPVEKCLRD+K+DK  +HDVVLVGG
Sbjct: 286 TTIEIDSLYEGIDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGG 345

Query: 241 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL 300
           STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAIL GEGNEKVQDLLLLDVTPL
Sbjct: 346 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPL 405

Query: 301 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 360
           SLG+ETAGG MTVLIPRNTTIPTKKEQVFSTYSDNQ  VLIQVYEGERARTKDNNLLG F
Sbjct: 406 SLGLETAGGGMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKF 465

Query: 361 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV 420
           EL GIPPAPRGVPQI VCFDIDANGILNV+AEDKT G KN ITITNDKGRLS++EIERMV
Sbjct: 466 ELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIERMV 525

Query: 421 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTVRDEKLAGKMNPAEKGKIEKAIDEAID 480
           QEAEKYK+EDE++KKKVEAKNALENYAYNMRNT++D+K+  +++ A+K +IE AIDEAI 
Sbjct: 526 QEAEKYKSEDEELKKKVEAKNALENYAYNMRNTIKDDKINSQLSAADKKRIEDAIDEAIK 585

Query: 481 WLERNQLAEVDEFEDKLKELEGLCNPIIAKLYQGGAGGDMPMGNGGGAGCSGGA--GAGP 540
           WL+ NQLAE DEFEDK+KELE +CNPIIAK+YQGGAGG     +G   G S G+  GAGP
Sbjct: 586 WLDNNQLAEADEFEDKMKELESICNPIIAKMYQGGAGGATMDEDGPSVGGSAGSQTGAGP 645

Query: 541 KIEEVD 545
           KIEEVD
Sbjct: 646 KIEEVD 651

BLAST of Lcy05g003590 vs. ExPASy Swiss-Prot
Match: Q9LHA8 (Heat shock 70 kDa protein 4 OS=Arabidopsis thaliana OX=3702 GN=HSP70-4 PE=1 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 3.0e-261
Identity = 467/548 (85.22%), Postives = 501/548 (91.42%), Query Frame = 0

Query: 1   MIVVNYKGEEKQFAPEEISSMVLIKMKEIAEAYLGHNVKNAVITVPAYFNDSQRQATKDA 60
           MIVVN+KGEEKQF+ EEISSMVLIKM+EIAEA+LG  VKNAV+TVPAYFNDSQRQATKDA
Sbjct: 106 MIVVNHKGEEKQFSAEEISSMVLIKMREIAEAFLGSPVKNAVVTVPAYFNDSQRQATKDA 165

Query: 61  GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 120
           G I+GLNV+RIINEPTAAAIAYGLDKKAS  GEKNVLIFDLGGGTFDVSLLTIEEGIFEV
Sbjct: 166 GVISGLNVMRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 225

Query: 121 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 180
           KATAGDTHLGGEDFDNR+VNHF  EFKRK+KKDI GN RALRRLRTACERAKRTLSST Q
Sbjct: 226 KATAGDTHLGGEDFDNRMVNHFVQEFKRKNKKDITGNPRALRRLRTACERAKRTLSSTAQ 285

Query: 181 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQIHDVVLVGG 240
           TTIEIDSL+EGIDFY TITRARFEELNMDLFR+CMEPVEKCLRD+K+DK  +HDVVLVGG
Sbjct: 286 TTIEIDSLFEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGG 345

Query: 241 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL 300
           STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAIL GEGNEKVQDLLLLDVTPL
Sbjct: 346 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPL 405

Query: 301 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 360
           SLG+ETAGGVMTVLIPRNTTIPTKKEQ+FSTYSDNQ  VLIQVYEGERARTKDNNLLG F
Sbjct: 406 SLGLETAGGVMTVLIPRNTTIPTKKEQIFSTYSDNQPGVLIQVYEGERARTKDNNLLGKF 465

Query: 361 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV 420
           EL GIPPAPRGVPQI VCFDIDANGILNV+AEDKT G KN ITITNDKGRLS++EIE+MV
Sbjct: 466 ELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKMV 525

Query: 421 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTVRDEKLAGKMNPAEKGKIEKAIDEAID 480
           QEAEKYKAEDE+ KKKV+AKNALENYAYNMRNT++DEK+A K++ A+K KIE AID+AI+
Sbjct: 526 QEAEKYKAEDEEHKKKVDAKNALENYAYNMRNTIKDEKIASKLDAADKKKIEDAIDQAIE 585

Query: 481 WLERNQLAEVDEFEDKLKELEGLCNPIIAKLYQGGAGGDMPMGNGGG----AGCSGGAGA 540
           WL+ NQLAE DEFEDK+KELE LCNPIIA++YQ GAG D  MG  GG        G  GA
Sbjct: 586 WLDGNQLAEADEFEDKMKELESLCNPIIARMYQ-GAGPD--MGGAGGMDDDTPAGGSGGA 645

Query: 541 GPKIEEVD 545
           GPKIEEVD
Sbjct: 646 GPKIEEVD 650

BLAST of Lcy05g003590 vs. ExPASy Swiss-Prot
Match: P27322 (Heat shock cognate 70 kDa protein 2 OS=Solanum lycopersicum OX=4081 GN=HSC-2 PE=2 SV=1)

HSP 1 Score: 894.8 bits (2311), Expect = 4.8e-259
Identity = 464/544 (85.29%), Postives = 499/544 (91.73%), Query Frame = 0

Query: 1   MIVVNYKGEEKQFAPEEISSMVLIKMKEIAEAYLGHNVKNAVITVPAYFNDSQRQATKDA 60
           MIVVNYKGEEKQF+ EEISSMVLIKMKEIAEA+LG  VKNAV+TVPAY NDSQRQATKDA
Sbjct: 106 MIVVNYKGEEKQFSAEEISSMVLIKMKEIAEAFLGTTVKNAVVTVPAYSNDSQRQATKDA 165

Query: 61  GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 120
           G I+GLNV+RIINEPTAAAIAYGLDKKA+  GEKNVLIFDLGGGTFDVSLLTIEEGIFEV
Sbjct: 166 GVISGLNVMRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 225

Query: 121 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 180
           KATAGDTHLGGEDFDNR+VNHF  EFKRK+KKDI GN RALRRLRTACERAKRTLSST Q
Sbjct: 226 KATAGDTHLGGEDFDNRMVNHFVQEFKRKNKKDITGNPRALRRLRTACERAKRTLSSTAQ 285

Query: 181 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQIHDVVLVGG 240
           TTIEIDSLYEGIDFY+TITRARFEELNMDLFR+CMEPVEKCLRD+K+DK  +HDVVLVGG
Sbjct: 286 TTIEIDSLYEGIDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGG 345

Query: 241 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL 300
           STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAIL GEGNEKVQDLLLLDVTPL
Sbjct: 346 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPL 405

Query: 301 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 360
           SLG+ETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQ  VLIQVYEGER RT+DNNLLG F
Sbjct: 406 SLGLETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERTRTRDNNLLGKF 465

Query: 361 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV 420
           EL GIPPAPRGVPQI VCFDIDANG LNV+AEDKT G KN ITITNDKGRLS++EIE+MV
Sbjct: 466 ELSGIPPAPRGVPQITVCFDIDANGTLNVSAEDKTTGQKNKITITNDKGRLSKEEIEKMV 525

Query: 421 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTVRDEKLAGKMNPAEKGKIEKAIDEAID 480
           QEAEKYK+EDE+ KKKVEAKNALENYAYNMRNT++DEK+A K++  ++ KIE AI++AI 
Sbjct: 526 QEAEKYKSEDEEHKKKVEAKNALENYAYNMRNTIKDEKIASKLSADDRTKIEDAIEQAIQ 585

Query: 481 WLERNQLAEVDEFEDKLKELEGLCNPIIAKLYQGGAGGDMPMGNGGGAGCSGGAGAGPKI 540
           WL+ NQLAE +EFEDK+KELE LCNPIIAK+YQ GAGGDM   +  G   SGG GAGPKI
Sbjct: 586 WLDGNQLAEAEEFEDKMKELESLCNPIIAKMYQ-GAGGDM---DDEGPAPSGG-GAGPKI 644

Query: 541 EEVD 545
           EEVD
Sbjct: 646 EEVD 644

BLAST of Lcy05g003590 vs. ExPASy TrEMBL
Match: A0A6P3YWY0 (heat shock cognate 70 kDa protein-like OS=Ziziphus jujuba OX=326968 GN=LOC107405906 PE=3 SV=1)

HSP 1 Score: 976.5 bits (2523), Expect = 4.6e-281
Identity = 504/545 (92.48%), Postives = 526/545 (96.51%), Query Frame = 0

Query: 1   MIVVNYKGEEKQFAPEEISSMVLIKMKEIAEAYLGHNVKNAVITVPAYFNDSQRQATKDA 60
           MIVVNYKGEEKQFA EEISSMVL KMKEIAEAYLG ++KNAVITVPAYFNDSQRQATKDA
Sbjct: 108 MIVVNYKGEEKQFAAEEISSMVLTKMKEIAEAYLGTSIKNAVITVPAYFNDSQRQATKDA 167

Query: 61  GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 120
           GAIAGLNV+RIINEPTAAAIAYGLDKKASRSGEK+VLIFDLGGGTFDVSLLTIEEGIFEV
Sbjct: 168 GAIAGLNVMRIINEPTAAAIAYGLDKKASRSGEKSVLIFDLGGGTFDVSLLTIEEGIFEV 227

Query: 121 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 180
           KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ
Sbjct: 228 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 287

Query: 181 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQIHDVVLVGG 240
           TTIEIDSLYEGIDFYATITRARFEELNMDLFR+CMEPVEKCL+D+KIDK Q+HD+VLVGG
Sbjct: 288 TTIEIDSLYEGIDFYATITRARFEELNMDLFRKCMEPVEKCLKDAKIDKSQVHDIVLVGG 347

Query: 241 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL 300
           STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAIL GEG++KVQDLLLLDVTPL
Sbjct: 348 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGDQKVQDLLLLDVTPL 407

Query: 301 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 360
           SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF
Sbjct: 408 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 467

Query: 361 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV 420
           ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKN ITITNDKGRLS+DEIERMV
Sbjct: 468 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNKITITNDKGRLSKDEIERMV 527

Query: 421 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTVRDEKLAGKMNPAEKGKIEKAIDEAID 480
           QEAEKYKAEDEQVKKKVEAKNALENYAYNMR T++D+  AGK++PA+K KIEKAIDE +D
Sbjct: 528 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRTTIKDDNFAGKLDPADKQKIEKAIDETVD 587

Query: 481 WLERNQLAEVDEFEDKLKELEGLCNPIIAKLYQ-GGAGGDMPMGNGGGAGCSGGAGAGPK 540
           W+ERNQLAEVDEFEDKLKELEGLCNPIIAKLYQ GGAG +MP G G G   SGG+GAGPK
Sbjct: 588 WVERNQLAEVDEFEDKLKELEGLCNPIIAKLYQGGGAGAEMP-GGGHGNASSGGSGAGPK 647

Query: 541 IEEVD 545
           IEEVD
Sbjct: 648 IEEVD 651

BLAST of Lcy05g003590 vs. ExPASy TrEMBL
Match: A0A6J1BIS9 (heat shock 70 kDa protein 5 OS=Herrania umbratica OX=108875 GN=LOC110427096 PE=3 SV=1)

HSP 1 Score: 975.3 bits (2520), Expect = 1.0e-280
Identity = 500/545 (91.74%), Postives = 522/545 (95.78%), Query Frame = 0

Query: 1   MIVVNYKGEEKQFAPEEISSMVLIKMKEIAEAYLGHNVKNAVITVPAYFNDSQRQATKDA 60
           MIVV YKGEEKQF+PEEISSMVL KMKE+AEAYLGH VKNAV+TVPAYFNDSQRQATKDA
Sbjct: 106 MIVVRYKGEEKQFSPEEISSMVLTKMKEVAEAYLGHTVKNAVVTVPAYFNDSQRQATKDA 165

Query: 61  GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 120
           GAIAGLNV+RIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEG+FEV
Sbjct: 166 GAIAGLNVIRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGVFEV 225

Query: 121 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 180
           KATAGDTHLGGEDFDNRLVNHF AEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ
Sbjct: 226 KATAGDTHLGGEDFDNRLVNHFVAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 285

Query: 181 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQIHDVVLVGG 240
           TTIEIDSLYEGIDFY+TITRARFEELNMDLFR+CMEPVEKCL DSKIDK Q+HDVVLVGG
Sbjct: 286 TTIEIDSLYEGIDFYSTITRARFEELNMDLFRKCMEPVEKCLSDSKIDKSQVHDVVLVGG 345

Query: 241 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL 300
           STRIPKVQQLLQ FFNGKELCKSINPDEAVAYGAAVQAAIL GEGN+KVQDLLLLDVTPL
Sbjct: 346 STRIPKVQQLLQGFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNDKVQDLLLLDVTPL 405

Query: 301 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 360
           SLGIETAGGVMTVLIPRNTT+PTKKEQVFSTYSDNQT VLIQVYEGERARTKDNNLLGTF
Sbjct: 406 SLGIETAGGVMTVLIPRNTTVPTKKEQVFSTYSDNQTGVLIQVYEGERARTKDNNLLGTF 465

Query: 361 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV 420
           ELKGIPPAPRGVPQINVCFDIDANGILNV+AEDKTAGVKN ITITNDKGRLS ++IERMV
Sbjct: 466 ELKGIPPAPRGVPQINVCFDIDANGILNVSAEDKTAGVKNQITITNDKGRLSIEDIERMV 525

Query: 421 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTVRDEKLAGKMNPAEKGKIEKAIDEAID 480
           QEAEKYKAEDEQVKKKVEAKN+LENYAYNMRN V+DEKLAGK+N A+K KIEKAIDE I+
Sbjct: 526 QEAEKYKAEDEQVKKKVEAKNSLENYAYNMRNAVKDEKLAGKLNLADKQKIEKAIDETIE 585

Query: 481 WLERNQLAEVDEFEDKLKELEGLCNPIIAKLYQG-GAGGDMPMGNGGGAGCSGGAGAGPK 540
           WL+RNQLAEVDEFEDKLKELEGLCNPII+K+YQG GAGGDMPMG G G   SGG+ A PK
Sbjct: 586 WLDRNQLAEVDEFEDKLKELEGLCNPIISKMYQGQGAGGDMPMGGGAGNASSGGSAADPK 645

Query: 541 IEEVD 545
           IEEVD
Sbjct: 646 IEEVD 650

BLAST of Lcy05g003590 vs. ExPASy TrEMBL
Match: A0A6J1CSN4 (LOW QUALITY PROTEIN: heat shock 70 kDa protein OS=Momordica charantia OX=3673 GN=LOC111014209 PE=3 SV=1)

HSP 1 Score: 972.2 bits (2512), Expect = 8.7e-280
Identity = 501/530 (94.53%), Postives = 509/530 (96.04%), Query Frame = 0

Query: 1   MIVVNYKGEEKQFAPEEISSMVLIKMKEIAEAYLGHNVKNAVITVPAYFNDSQRQATKDA 60
           MIVV YKGEEKQFA EEISSMVLIKM+EIAEAYLGHNV NAVITVPAYFNDSQRQATKDA
Sbjct: 106 MIVVQYKGEEKQFAAEEISSMVLIKMEEIAEAYLGHNVNNAVITVPAYFNDSQRQATKDA 165

Query: 61  GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 120
           GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIE GIFEV
Sbjct: 166 GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEXGIFEV 225

Query: 121 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 180
           KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDI GN RALRRLRTACERAKRTLSSTTQ
Sbjct: 226 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTTQ 285

Query: 181 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQIHDVVLVGG 240
           TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQ+HDVVLVGG
Sbjct: 286 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQVHDVVLVGG 345

Query: 241 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL 300
           ST IPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL
Sbjct: 346 STXIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL 405

Query: 301 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 360
           SLGIETAGGVMTVLIPRNTT+P KKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF
Sbjct: 406 SLGIETAGGVMTVLIPRNTTVPKKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 465

Query: 361 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV 420
           ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV
Sbjct: 466 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV 525

Query: 421 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTVRDEKLAGKMNPAEKGKIEKAIDEAID 480
           QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNT +DEKLAGK+NPAEK KIEKAIDEA++
Sbjct: 526 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTAKDEKLAGKLNPAEKAKIEKAIDEAME 585

Query: 481 WLERNQLAEVDEFEDKLKELEGLCNPIIAKLYQGGAGGDMPMGNGGGAGC 531
           WLERNQLAEVDEFEDKLKELEG+CNPIIAKLYQG          GGG GC
Sbjct: 586 WLERNQLAEVDEFEDKLKELEGICNPIIAKLYQGA---------GGGGGC 626

BLAST of Lcy05g003590 vs. ExPASy TrEMBL
Match: W9QV79 (Heat shock 70 kDa protein OS=Morus notabilis OX=981085 GN=L484_018081 PE=3 SV=1)

HSP 1 Score: 966.8 bits (2498), Expect = 3.7e-278
Identity = 500/551 (90.74%), Postives = 521/551 (94.56%), Query Frame = 0

Query: 1   MIVVNYKGEEKQFAPEEISSMVLIKMKEIAEAYLGHNVKNAVITVPAYFNDSQRQATKDA 60
           MIVV+YKGEEKQFA EEISSMVL KMKEIAEAYLG +VKNAVITVPAYFNDSQRQATKDA
Sbjct: 106 MIVVHYKGEEKQFAAEEISSMVLTKMKEIAEAYLGQSVKNAVITVPAYFNDSQRQATKDA 165

Query: 61  GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 120
           GAIAGLNVLRIINEPTAAAIAYGLDKKAS SGEKNVLIFDLGGGTFDVSLLTIEEGIFEV
Sbjct: 166 GAIAGLNVLRIINEPTAAAIAYGLDKKASSSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 225

Query: 121 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 180
           KATAGDTHLGGEDFDNRLVNHF AEFKRKHKKDI GNARALRRLRTACERAKRTLSSTTQ
Sbjct: 226 KATAGDTHLGGEDFDNRLVNHFVAEFKRKHKKDISGNARALRRLRTACERAKRTLSSTTQ 285

Query: 181 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQIHDVVLVGG 240
           TTIEIDSLYEG DFY+TITRARFEE+NMDLFR+CMEPVEKCLRDSKIDK Q+HDVVLVGG
Sbjct: 286 TTIEIDSLYEGTDFYSTITRARFEEMNMDLFRKCMEPVEKCLRDSKIDKSQVHDVVLVGG 345

Query: 241 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL 300
           STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAIL GEGN+KVQDLLLLDVTPL
Sbjct: 346 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILTGEGNQKVQDLLLLDVTPL 405

Query: 301 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 360
           SLGIETAGGVMTVLIPRNTTIP KKEQ+FSTYSDNQ  VLIQVYEGERARTKDNNLLG F
Sbjct: 406 SLGIETAGGVMTVLIPRNTTIPAKKEQIFSTYSDNQPGVLIQVYEGERARTKDNNLLGKF 465

Query: 361 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV 420
           EL GIPPAPRGVPQINVCFDIDANGILNV+AEDKTAGVKN ITITNDKGRLS++EIERMV
Sbjct: 466 ELTGIPPAPRGVPQINVCFDIDANGILNVSAEDKTAGVKNKITITNDKGRLSKEEIERMV 525

Query: 421 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTVRDEKLAGKMNPAEKGKIEKAIDEAID 480
           QEAE YKAEDE+VKKKVEAKN+LENYAYNMRNT+RDEK AGK+ PA+K KIEKAIDEAI+
Sbjct: 526 QEAENYKAEDEEVKKKVEAKNSLENYAYNMRNTIRDEKFAGKLEPADKQKIEKAIDEAIE 585

Query: 481 WLERNQLAEVDEFEDKLKELEGLCNPIIAKLYQGGAGGDMPMG------NGG-GAGCSGG 540
           WL+RNQLAEVDE EDKLKELEGLCNPIIAK+YQGGAGGD+PMG      NGG G   SGG
Sbjct: 586 WLDRNQLAEVDELEDKLKELEGLCNPIIAKMYQGGAGGDVPMGGGAEMPNGGYGKASSGG 645

Query: 541 AGAGPKIEEVD 545
           +GAGPKIEEVD
Sbjct: 646 SGAGPKIEEVD 656

BLAST of Lcy05g003590 vs. ExPASy TrEMBL
Match: A0A059AJT0 (Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_J03127 PE=3 SV=1)

HSP 1 Score: 966.5 bits (2497), Expect = 4.8e-278
Identity = 501/551 (90.93%), Postives = 525/551 (95.28%), Query Frame = 0

Query: 1   MIVVNYKGEEKQFAPEEISSMVLIKMKEIAEAYLGHNVKNAVITVPAYFNDSQRQATKDA 60
           MIVV +KGEEKQF+PEEISSMVL KMKEIAEA+LGH +KNAVITVPAYFNDSQRQATKDA
Sbjct: 102 MIVVTFKGEEKQFSPEEISSMVLTKMKEIAEAFLGHPIKNAVITVPAYFNDSQRQATKDA 161

Query: 61  GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 120
           GAIAG+NVLRIINEPTAAAIAYGLDKKA+R+GEKNVLIFDLGGGTFDVSLLTIEEGIFEV
Sbjct: 162 GAIAGMNVLRIINEPTAAAIAYGLDKKAARAGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 221

Query: 121 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 180
           KATAGDTHLGGEDFDNRLVNHF  EFKRKHKKDI GNARALRRLRTACERAKRTLSST+Q
Sbjct: 222 KATAGDTHLGGEDFDNRLVNHFVQEFKRKHKKDISGNARALRRLRTACERAKRTLSSTSQ 281

Query: 181 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQIHDVVLVGG 240
           TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRD+KIDK Q+HDVVLVGG
Sbjct: 282 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDAKIDKSQVHDVVLVGG 341

Query: 241 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL 300
           STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAIL GEGNE+VQDLLLLDVTPL
Sbjct: 342 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNERVQDLLLLDVTPL 401

Query: 301 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 360
           SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF
Sbjct: 402 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 461

Query: 361 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV 420
           ELKGIPPAPRGVPQINVCFDIDANGILNV+AEDKTAGVKN ITITNDKGRLS+++IERMV
Sbjct: 462 ELKGIPPAPRGVPQINVCFDIDANGILNVSAEDKTAGVKNQITITNDKGRLSKEDIERMV 521

Query: 421 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTVRDEKLAGKMNPAEKGKIEKAIDEAID 480
           QEAEKYKAEDE VKKKVEAKNALENYAYNMRNTVRD+K AGK++PA+K KIEKAIDEAI+
Sbjct: 522 QEAEKYKAEDEGVKKKVEAKNALENYAYNMRNTVRDDKFAGKLDPADKQKIEKAIDEAIE 581

Query: 481 WLERNQLAEVDEFEDKLKELEGLCNPIIAKLYQGG-AGGDMPMGN--GGGAG----CSGG 540
           WL+RNQLAEVDE EDKLKELEGLCNPIIAK+YQGG  GGD+PMG+  GGG G       G
Sbjct: 582 WLDRNQLAEVDELEDKLKELEGLCNPIIAKMYQGGPGGGDVPMGDMPGGGYGKPKSGGSG 641

Query: 541 AGAGPKIEEVD 545
            GAGPKIEEVD
Sbjct: 642 GGAGPKIEEVD 652

BLAST of Lcy05g003590 vs. NCBI nr
Match: KAF3450992.1 (hypothetical protein FNV43_RR07081 [Rhamnella rubrinervis])

HSP 1 Score: 976.9 bits (2524), Expect = 7.3e-281
Identity = 505/553 (91.32%), Postives = 526/553 (95.12%), Query Frame = 0

Query: 1   MIVVNYKGEEKQFAPEEISSMVLIKMKEIAEAYLGHNVKNAVITVPAYFNDSQRQATKDA 60
           MIVVNYKGEEKQFAPEEISSMVL KMKEIAEAYLG  VKNAVITVPAYFNDSQRQATKDA
Sbjct: 106 MIVVNYKGEEKQFAPEEISSMVLTKMKEIAEAYLGTTVKNAVITVPAYFNDSQRQATKDA 165

Query: 61  GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 120
           GAIAGL V+RIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV
Sbjct: 166 GAIAGLTVMRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 225

Query: 121 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 180
           KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDI GNARALRRLRTACERAKRTLSSTTQ
Sbjct: 226 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDISGNARALRRLRTACERAKRTLSSTTQ 285

Query: 181 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQIHDVVLVGG 240
           TTIEIDSLYEG+DFYATITRARFEELNMDLFR+CMEPVEKCLRDSKIDK Q+HD+VLVGG
Sbjct: 286 TTIEIDSLYEGVDFYATITRARFEELNMDLFRKCMEPVEKCLRDSKIDKSQVHDIVLVGG 345

Query: 241 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL 300
           STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAIL GEGN+KVQDLLLLDVTPL
Sbjct: 346 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILGGEGNQKVQDLLLLDVTPL 405

Query: 301 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 360
           SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTY+DNQTSVLIQVYEGER RTKDNNLLGTF
Sbjct: 406 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYADNQTSVLIQVYEGERTRTKDNNLLGTF 465

Query: 361 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV 420
           ELKGIPPAPRGVPQINVCFDIDANGILNV+AEDKTAGVKN ITITNDKGRLS+D+IERMV
Sbjct: 466 ELKGIPPAPRGVPQINVCFDIDANGILNVSAEDKTAGVKNKITITNDKGRLSKDDIERMV 525

Query: 421 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTVRDEKLAGKMNPAEKGKIEKAIDEAID 480
           QEAEKY+AEDEQVKKKVEAKN+LENYAYNMRNTVR EK AGK++PA+K KIEKAIDE  +
Sbjct: 526 QEAEKYRAEDEQVKKKVEAKNSLENYAYNMRNTVRVEKFAGKLDPADKQKIEKAIDETTE 585

Query: 481 WLERNQLAEVDEFEDKLKELEGLCNPIIAKLYQGGAGGDMPMGNGGGAG---------CS 540
           WLERNQLAEVDEFEDKLKELEGLCNPI+AK+YQ G+GGD+PMG G GAG          S
Sbjct: 586 WLERNQLAEVDEFEDKLKELEGLCNPIVAKMYQ-GSGGDVPMGGGAGAGMPGGGYGKASS 645

Query: 541 GGAGAGPKIEEVD 545
           GG+GAGPKIEEVD
Sbjct: 646 GGSGAGPKIEEVD 657

BLAST of Lcy05g003590 vs. NCBI nr
Match: XP_015868485.1 (heat shock cognate 70 kDa protein-like [Ziziphus jujuba])

HSP 1 Score: 976.5 bits (2523), Expect = 9.6e-281
Identity = 504/545 (92.48%), Postives = 526/545 (96.51%), Query Frame = 0

Query: 1   MIVVNYKGEEKQFAPEEISSMVLIKMKEIAEAYLGHNVKNAVITVPAYFNDSQRQATKDA 60
           MIVVNYKGEEKQFA EEISSMVL KMKEIAEAYLG ++KNAVITVPAYFNDSQRQATKDA
Sbjct: 108 MIVVNYKGEEKQFAAEEISSMVLTKMKEIAEAYLGTSIKNAVITVPAYFNDSQRQATKDA 167

Query: 61  GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 120
           GAIAGLNV+RIINEPTAAAIAYGLDKKASRSGEK+VLIFDLGGGTFDVSLLTIEEGIFEV
Sbjct: 168 GAIAGLNVMRIINEPTAAAIAYGLDKKASRSGEKSVLIFDLGGGTFDVSLLTIEEGIFEV 227

Query: 121 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 180
           KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ
Sbjct: 228 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 287

Query: 181 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQIHDVVLVGG 240
           TTIEIDSLYEGIDFYATITRARFEELNMDLFR+CMEPVEKCL+D+KIDK Q+HD+VLVGG
Sbjct: 288 TTIEIDSLYEGIDFYATITRARFEELNMDLFRKCMEPVEKCLKDAKIDKSQVHDIVLVGG 347

Query: 241 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL 300
           STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAIL GEG++KVQDLLLLDVTPL
Sbjct: 348 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGDQKVQDLLLLDVTPL 407

Query: 301 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 360
           SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF
Sbjct: 408 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 467

Query: 361 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV 420
           ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKN ITITNDKGRLS+DEIERMV
Sbjct: 468 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNKITITNDKGRLSKDEIERMV 527

Query: 421 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTVRDEKLAGKMNPAEKGKIEKAIDEAID 480
           QEAEKYKAEDEQVKKKVEAKNALENYAYNMR T++D+  AGK++PA+K KIEKAIDE +D
Sbjct: 528 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRTTIKDDNFAGKLDPADKQKIEKAIDETVD 587

Query: 481 WLERNQLAEVDEFEDKLKELEGLCNPIIAKLYQ-GGAGGDMPMGNGGGAGCSGGAGAGPK 540
           W+ERNQLAEVDEFEDKLKELEGLCNPIIAKLYQ GGAG +MP G G G   SGG+GAGPK
Sbjct: 588 WVERNQLAEVDEFEDKLKELEGLCNPIIAKLYQGGGAGAEMP-GGGHGNASSGGSGAGPK 647

Query: 541 IEEVD 545
           IEEVD
Sbjct: 648 IEEVD 651

BLAST of Lcy05g003590 vs. NCBI nr
Match: XP_021298179.1 (heat shock 70 kDa protein 5 [Herrania umbratica])

HSP 1 Score: 975.3 bits (2520), Expect = 2.1e-280
Identity = 500/545 (91.74%), Postives = 522/545 (95.78%), Query Frame = 0

Query: 1   MIVVNYKGEEKQFAPEEISSMVLIKMKEIAEAYLGHNVKNAVITVPAYFNDSQRQATKDA 60
           MIVV YKGEEKQF+PEEISSMVL KMKE+AEAYLGH VKNAV+TVPAYFNDSQRQATKDA
Sbjct: 106 MIVVRYKGEEKQFSPEEISSMVLTKMKEVAEAYLGHTVKNAVVTVPAYFNDSQRQATKDA 165

Query: 61  GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 120
           GAIAGLNV+RIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEG+FEV
Sbjct: 166 GAIAGLNVIRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGVFEV 225

Query: 121 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 180
           KATAGDTHLGGEDFDNRLVNHF AEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ
Sbjct: 226 KATAGDTHLGGEDFDNRLVNHFVAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 285

Query: 181 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQIHDVVLVGG 240
           TTIEIDSLYEGIDFY+TITRARFEELNMDLFR+CMEPVEKCL DSKIDK Q+HDVVLVGG
Sbjct: 286 TTIEIDSLYEGIDFYSTITRARFEELNMDLFRKCMEPVEKCLSDSKIDKSQVHDVVLVGG 345

Query: 241 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL 300
           STRIPKVQQLLQ FFNGKELCKSINPDEAVAYGAAVQAAIL GEGN+KVQDLLLLDVTPL
Sbjct: 346 STRIPKVQQLLQGFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNDKVQDLLLLDVTPL 405

Query: 301 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 360
           SLGIETAGGVMTVLIPRNTT+PTKKEQVFSTYSDNQT VLIQVYEGERARTKDNNLLGTF
Sbjct: 406 SLGIETAGGVMTVLIPRNTTVPTKKEQVFSTYSDNQTGVLIQVYEGERARTKDNNLLGTF 465

Query: 361 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV 420
           ELKGIPPAPRGVPQINVCFDIDANGILNV+AEDKTAGVKN ITITNDKGRLS ++IERMV
Sbjct: 466 ELKGIPPAPRGVPQINVCFDIDANGILNVSAEDKTAGVKNQITITNDKGRLSIEDIERMV 525

Query: 421 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTVRDEKLAGKMNPAEKGKIEKAIDEAID 480
           QEAEKYKAEDEQVKKKVEAKN+LENYAYNMRN V+DEKLAGK+N A+K KIEKAIDE I+
Sbjct: 526 QEAEKYKAEDEQVKKKVEAKNSLENYAYNMRNAVKDEKLAGKLNLADKQKIEKAIDETIE 585

Query: 481 WLERNQLAEVDEFEDKLKELEGLCNPIIAKLYQG-GAGGDMPMGNGGGAGCSGGAGAGPK 540
           WL+RNQLAEVDEFEDKLKELEGLCNPII+K+YQG GAGGDMPMG G G   SGG+ A PK
Sbjct: 586 WLDRNQLAEVDEFEDKLKELEGLCNPIISKMYQGQGAGGDMPMGGGAGNASSGGSAADPK 645

Query: 541 IEEVD 545
           IEEVD
Sbjct: 646 IEEVD 650

BLAST of Lcy05g003590 vs. NCBI nr
Match: XP_022144559.1 (LOW QUALITY PROTEIN: heat shock 70 kDa protein [Momordica charantia])

HSP 1 Score: 972.2 bits (2512), Expect = 1.8e-279
Identity = 501/530 (94.53%), Postives = 509/530 (96.04%), Query Frame = 0

Query: 1   MIVVNYKGEEKQFAPEEISSMVLIKMKEIAEAYLGHNVKNAVITVPAYFNDSQRQATKDA 60
           MIVV YKGEEKQFA EEISSMVLIKM+EIAEAYLGHNV NAVITVPAYFNDSQRQATKDA
Sbjct: 106 MIVVQYKGEEKQFAAEEISSMVLIKMEEIAEAYLGHNVNNAVITVPAYFNDSQRQATKDA 165

Query: 61  GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 120
           GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIE GIFEV
Sbjct: 166 GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEXGIFEV 225

Query: 121 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 180
           KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDI GN RALRRLRTACERAKRTLSSTTQ
Sbjct: 226 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDISGNPRALRRLRTACERAKRTLSSTTQ 285

Query: 181 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQIHDVVLVGG 240
           TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQ+HDVVLVGG
Sbjct: 286 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQVHDVVLVGG 345

Query: 241 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL 300
           ST IPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL
Sbjct: 346 STXIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL 405

Query: 301 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 360
           SLGIETAGGVMTVLIPRNTT+P KKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF
Sbjct: 406 SLGIETAGGVMTVLIPRNTTVPKKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 465

Query: 361 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV 420
           ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV
Sbjct: 466 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV 525

Query: 421 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTVRDEKLAGKMNPAEKGKIEKAIDEAID 480
           QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNT +DEKLAGK+NPAEK KIEKAIDEA++
Sbjct: 526 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTAKDEKLAGKLNPAEKAKIEKAIDEAME 585

Query: 481 WLERNQLAEVDEFEDKLKELEGLCNPIIAKLYQGGAGGDMPMGNGGGAGC 531
           WLERNQLAEVDEFEDKLKELEG+CNPIIAKLYQG          GGG GC
Sbjct: 586 WLERNQLAEVDEFEDKLKELEGICNPIIAKLYQGA---------GGGGGC 626

BLAST of Lcy05g003590 vs. NCBI nr
Match: XP_031273683.1 (heat shock 70 kDa protein-like [Pistacia vera])

HSP 1 Score: 969.5 bits (2505), Expect = 1.2e-278
Identity = 501/550 (91.09%), Postives = 524/550 (95.27%), Query Frame = 0

Query: 1   MIVVNYKGEEKQFAPEEISSMVLIKMKEIAEAYLGHNVKNAVITVPAYFNDSQRQATKDA 60
           MIVV+YKGEEKQFA EEISSMVL KM+EIAEAYLG NVKNAVITVPAYFNDSQRQATKDA
Sbjct: 106 MIVVSYKGEEKQFAAEEISSMVLTKMREIAEAYLGQNVKNAVITVPAYFNDSQRQATKDA 165

Query: 61  GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 120
           GAIAGLNV+RIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV
Sbjct: 166 GAIAGLNVMRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 225

Query: 121 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 180
           KATAGDTHLGGEDFDNRLVNHF AEFKRKHKKDI GNARALRRLRTACERAKRTLSSTTQ
Sbjct: 226 KATAGDTHLGGEDFDNRLVNHFVAEFKRKHKKDISGNARALRRLRTACERAKRTLSSTTQ 285

Query: 181 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQIHDVVLVGG 240
           TTIEIDSLYEGIDFY+TITRARFEELNMDLFR+CMEPVEKCLRDSKIDK Q+HD+VLVGG
Sbjct: 286 TTIEIDSLYEGIDFYSTITRARFEELNMDLFRKCMEPVEKCLRDSKIDKSQVHDIVLVGG 345

Query: 241 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL 300
           STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAIL GEGN+KVQDLLLLDVTPL
Sbjct: 346 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNDKVQDLLLLDVTPL 405

Query: 301 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 360
           SLGIETAGGVMTVLIPRNTTIPTKKEQ+FSTYSDNQ  VLIQVYEGERARTKDNNLLG F
Sbjct: 406 SLGIETAGGVMTVLIPRNTTIPTKKEQIFSTYSDNQPGVLIQVYEGERARTKDNNLLGKF 465

Query: 361 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV 420
           EL GIPPAPRGVPQINVCFDIDANGILNV+AEDKTAGVKN ITITNDKGRLS++EIERMV
Sbjct: 466 ELTGIPPAPRGVPQINVCFDIDANGILNVSAEDKTAGVKNKITITNDKGRLSKEEIERMV 525

Query: 421 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTVRDEKLAGKMNPAEKGKIEKAIDEAID 480
           QEAEKYKAEDE+VKKKV+AKNALENYAYNMRNTV+DEK AGK++PA+K KIEKAIDEAI+
Sbjct: 526 QEAEKYKAEDEEVKKKVDAKNALENYAYNMRNTVKDEKFAGKLDPADKRKIEKAIDEAIE 585

Query: 481 WLERNQLAEVDEFEDKLKELEGLCNPIIAKLYQGGAGGDMPMG------NGGGAGCSGGA 540
           WL+RNQLAEV+E EDKLKELEGLCNPIIAK+YQ GAGGD+PMG      NG G   SGGA
Sbjct: 586 WLDRNQLAEVEELEDKLKELEGLCNPIIAKMYQ-GAGGDVPMGGGAEMPNGYGKASSGGA 645

Query: 541 GAGPKIEEVD 545
           GAGPKIEEVD
Sbjct: 646 GAGPKIEEVD 654

BLAST of Lcy05g003590 vs. TAIR 10
Match: AT1G16030.1 (heat shock protein 70B )

HSP 1 Score: 912.5 bits (2357), Expect = 1.6e-265
Identity = 465/545 (85.32%), Postives = 507/545 (93.03%), Query Frame = 0

Query: 1   MIVVNYKGEEKQFAPEEISSMVLIKMKEIAEAYLGHNVKNAVITVPAYFNDSQRQATKDA 60
           MIVV+YK EEKQF+PEEISSMVL+KMKE+AEA+LG  VKNAV+TVPAYFNDSQRQATKDA
Sbjct: 105 MIVVSYKNEEKQFSPEEISSMVLVKMKEVAEAFLGRTVKNAVVTVPAYFNDSQRQATKDA 164

Query: 61  GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 120
           GAI+GLNVLRIINEPTAAAIAYGLDKK +++GEKNVLIFDLGGGTFDVSLLTIEEG+FEV
Sbjct: 165 GAISGLNVLRIINEPTAAAIAYGLDKKGTKAGEKNVLIFDLGGGTFDVSLLTIEEGVFEV 224

Query: 121 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 180
           KATAGDTHLGGEDFDNRLVNHF AEF+RKHKKDI GNARALRRLRTACERAKRTLSST Q
Sbjct: 225 KATAGDTHLGGEDFDNRLVNHFVAEFRRKHKKDIAGNARALRRLRTACERAKRTLSSTAQ 284

Query: 181 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQIHDVVLVGG 240
           TTIEIDSL+EGIDFYATI+RARFEE+NMDLFR+CM+PVEK L+D+K+DK  +HDVVLVGG
Sbjct: 285 TTIEIDSLHEGIDFYATISRARFEEMNMDLFRKCMDPVEKVLKDAKLDKSSVHDVVLVGG 344

Query: 241 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL 300
           STRIPK+QQLLQDFFNGKELCKSINPDEAVAYGAAVQAAIL GEG+EKVQDLLLLDV PL
Sbjct: 345 STRIPKIQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILTGEGSEKVQDLLLLDVAPL 404

Query: 301 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 360
           SLG+ETAGGVMTVLIPRNTT+P KKEQVFSTY+DNQ  VLIQVYEGERART+DNNLLGTF
Sbjct: 405 SLGLETAGGVMTVLIPRNTTVPCKKEQVFSTYADNQPGVLIQVYEGERARTRDNNLLGTF 464

Query: 361 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV 420
           ELKGIPPAPRGVPQINVCFDIDANGILNV+AEDKTAGVKN ITITNDKGRLS++EIE+MV
Sbjct: 465 ELKGIPPAPRGVPQINVCFDIDANGILNVSAEDKTAGVKNQITITNDKGRLSKEEIEKMV 524

Query: 421 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTVRDEKLAGKMNPAEKGKIEKAIDEAID 480
           Q+AEKYKAEDEQVKKKVEAKN+LENYAYNMRNT++DEKLA K+   +K KIEKAIDE I+
Sbjct: 525 QDAEKYKAEDEQVKKKVEAKNSLENYAYNMRNTIKDEKLAQKLTQEDKQKIEKAIDETIE 584

Query: 481 WLERNQLAEVDEFEDKLKELEGLCNPIIAKLYQGG-AGGDMPMGNGGGAGCSGGAGAGPK 540
           W+E NQLAEVDEFE KLKELEG+CNPII+K+YQGG A G MP     G   S GA  GPK
Sbjct: 585 WIEGNQLAEVDEFEYKLKELEGICNPIISKMYQGGAAAGGMPT---DGDFSSSGAAGGPK 644

Query: 541 IEEVD 545
           IEEVD
Sbjct: 645 IEEVD 646

BLAST of Lcy05g003590 vs. TAIR 10
Match: AT3G12580.1 (heat shock protein 70 )

HSP 1 Score: 902.1 bits (2330), Expect = 2.1e-262
Identity = 467/548 (85.22%), Postives = 501/548 (91.42%), Query Frame = 0

Query: 1   MIVVNYKGEEKQFAPEEISSMVLIKMKEIAEAYLGHNVKNAVITVPAYFNDSQRQATKDA 60
           MIVVN+KGEEKQF+ EEISSMVLIKM+EIAEA+LG  VKNAV+TVPAYFNDSQRQATKDA
Sbjct: 106 MIVVNHKGEEKQFSAEEISSMVLIKMREIAEAFLGSPVKNAVVTVPAYFNDSQRQATKDA 165

Query: 61  GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 120
           G I+GLNV+RIINEPTAAAIAYGLDKKAS  GEKNVLIFDLGGGTFDVSLLTIEEGIFEV
Sbjct: 166 GVISGLNVMRIINEPTAAAIAYGLDKKASSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 225

Query: 121 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 180
           KATAGDTHLGGEDFDNR+VNHF  EFKRK+KKDI GN RALRRLRTACERAKRTLSST Q
Sbjct: 226 KATAGDTHLGGEDFDNRMVNHFVQEFKRKNKKDITGNPRALRRLRTACERAKRTLSSTAQ 285

Query: 181 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQIHDVVLVGG 240
           TTIEIDSL+EGIDFY TITRARFEELNMDLFR+CMEPVEKCLRD+K+DK  +HDVVLVGG
Sbjct: 286 TTIEIDSLFEGIDFYTTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSSVHDVVLVGG 345

Query: 241 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL 300
           STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAIL GEGNEKVQDLLLLDVTPL
Sbjct: 346 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPL 405

Query: 301 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 360
           SLG+ETAGGVMTVLIPRNTTIPTKKEQ+FSTYSDNQ  VLIQVYEGERARTKDNNLLG F
Sbjct: 406 SLGLETAGGVMTVLIPRNTTIPTKKEQIFSTYSDNQPGVLIQVYEGERARTKDNNLLGKF 465

Query: 361 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV 420
           EL GIPPAPRGVPQI VCFDIDANGILNV+AEDKT G KN ITITNDKGRLS++EIE+MV
Sbjct: 466 ELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKEEIEKMV 525

Query: 421 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTVRDEKLAGKMNPAEKGKIEKAIDEAID 480
           QEAEKYKAEDE+ KKKV+AKNALENYAYNMRNT++DEK+A K++ A+K KIE AID+AI+
Sbjct: 526 QEAEKYKAEDEEHKKKVDAKNALENYAYNMRNTIKDEKIASKLDAADKKKIEDAIDQAIE 585

Query: 481 WLERNQLAEVDEFEDKLKELEGLCNPIIAKLYQGGAGGDMPMGNGGG----AGCSGGAGA 540
           WL+ NQLAE DEFEDK+KELE LCNPIIA++YQ GAG D  MG  GG        G  GA
Sbjct: 586 WLDGNQLAEADEFEDKMKELESLCNPIIARMYQ-GAGPD--MGGAGGMDDDTPAGGSGGA 645

Query: 541 GPKIEEVD 545
           GPKIEEVD
Sbjct: 646 GPKIEEVD 650

BLAST of Lcy05g003590 vs. TAIR 10
Match: AT5G02500.1 (heat shock cognate protein 70-1 )

HSP 1 Score: 892.9 bits (2306), Expect = 1.3e-259
Identity = 464/552 (84.06%), Postives = 495/552 (89.67%), Query Frame = 0

Query: 1   MIVVNYKGEEKQFAPEEISSMVLIKMKEIAEAYLGHNVKNAVITVPAYFNDSQRQATKDA 60
           MI V YKGEEK+FA EEISSMVLIKM+EIAEAYLG  +KNAV+TVPAYFNDSQRQATKDA
Sbjct: 106 MIYVEYKGEEKEFAAEEISSMVLIKMREIAEAYLGVTIKNAVVTVPAYFNDSQRQATKDA 165

Query: 61  GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 120
           G IAGLNV+RIINEPTAAAIAYGLDKKA+  GEKNVLIFDLGGGTFDVSLLTIEEGIFEV
Sbjct: 166 GVIAGLNVMRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 225

Query: 121 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 180
           KATAGDTHLGGEDFDNR+VNHF  EFKRK KKDI GN RALRRLRT+CERAKRTLSST Q
Sbjct: 226 KATAGDTHLGGEDFDNRMVNHFVQEFKRKSKKDITGNPRALRRLRTSCERAKRTLSSTAQ 285

Query: 181 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQIHDVVLVGG 240
           TTIEIDSLYEGIDFY+TITRARFEELNMDLFR+CMEPVEKCLRD+K+DK  +HDVVLVGG
Sbjct: 286 TTIEIDSLYEGIDFYSTITRARFEELNMDLFRKCMEPVEKCLRDAKMDKSTVHDVVLVGG 345

Query: 241 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL 300
           STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQ AIL GEGNEKVQDLLLLDVTPL
Sbjct: 346 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQGAILSGEGNEKVQDLLLLDVTPL 405

Query: 301 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 360
           SLG+ETAGGVMT LIPRNTTIPTKKEQVFSTYSDNQ  VLIQVYEGERARTKDNNLLG F
Sbjct: 406 SLGLETAGGVMTTLIPRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKF 465

Query: 361 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV 420
           EL GIPPAPRGVPQI VCFDIDANGILNV+AEDKT G KN ITITNDKGRLS+DEIE+MV
Sbjct: 466 ELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKDEIEKMV 525

Query: 421 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTVRDEKLAGKMNPAEKGKIEKAIDEAID 480
           QEAEKYK+EDE+ KKKVEAKNALENYAYNMRNT++DEK+  K+  A+K KIE +I++AI 
Sbjct: 526 QEAEKYKSEDEEHKKKVEAKNALENYAYNMRNTIQDEKIGEKLPAADKKKIEDSIEQAIQ 585

Query: 481 WLERNQLAEVDEFEDKLKELEGLCNPIIAKLYQGGAGGDMPMGNGGGAGCSG-------- 540
           WLE NQLAE DEFEDK+KELE +CNPIIAK+YQ GAGG+      GG G SG        
Sbjct: 586 WLEGNQLAEADEFEDKMKELESICNPIIAKMYQ-GAGGE-----AGGPGASGMDDDAPPA 645

Query: 541 GAGAGPKIEEVD 545
             GAGPKIEEVD
Sbjct: 646 SGGAGPKIEEVD 651

BLAST of Lcy05g003590 vs. TAIR 10
Match: AT5G02490.1 (Heat shock protein 70 (Hsp 70) family protein )

HSP 1 Score: 884.0 bits (2283), Expect = 6.0e-257
Identity = 457/553 (82.64%), Postives = 493/553 (89.15%), Query Frame = 0

Query: 1   MIVVNYKGEEKQFAPEEISSMVLIKMKEIAEAYLGHNVKNAVITVPAYFNDSQRQATKDA 60
           MIVV YKGEEKQFA EEISSMVLIKM+EIAEA+LG  VKNAV+TVPAYFNDSQRQATKDA
Sbjct: 106 MIVVEYKGEEKQFAAEEISSMVLIKMREIAEAFLGTTVKNAVVTVPAYFNDSQRQATKDA 165

Query: 61  GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 120
           G IAGLNVLRIINEPTAAAIAYGLDKKA+  GEKNVLIFDLGGGTFDVSLLTIEEGIFEV
Sbjct: 166 GVIAGLNVLRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 225

Query: 121 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 180
           KATAGDTHLGGEDFDNR+VNHF  EFKRK+K+DI G  RALRRLRTACERAKRTLSST Q
Sbjct: 226 KATAGDTHLGGEDFDNRMVNHFVQEFKRKNKQDITGQPRALRRLRTACERAKRTLSSTAQ 285

Query: 181 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQIHDVVLVGG 240
           TTIEIDSLY G DFY+ ITRARFEE+NMDLFR+CMEPVEKCLRD+K+DK  +H++VLVGG
Sbjct: 286 TTIEIDSLYGGADFYSPITRARFEEMNMDLFRKCMEPVEKCLRDAKMDKSTVHEIVLVGG 345

Query: 241 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL 300
           STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAIL GEGNEKVQDLLLLDVTPL
Sbjct: 346 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPL 405

Query: 301 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 360
           SLG+ETAGGVMT LI RNTTIPTKKEQVFSTYSDNQ  VLIQV+EGERARTKDNNLLG F
Sbjct: 406 SLGLETAGGVMTTLIQRNTTIPTKKEQVFSTYSDNQPGVLIQVFEGERARTKDNNLLGKF 465

Query: 361 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV 420
           EL GIPPAPRGVPQI VCFDIDANGILNV+AEDKT G KN ITITNDKGRLS+++IE+MV
Sbjct: 466 ELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGKKNKITITNDKGRLSKEDIEKMV 525

Query: 421 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTVRDEKLAGKMNPAEKGKIEKAIDEAID 480
           QEAEKYK+EDE+ KKKVEAKNALENYAYNMRNT+RDEK+  K+  A+K K+E +I+EAI 
Sbjct: 526 QEAEKYKSEDEEHKKKVEAKNALENYAYNMRNTIRDEKIGEKLPAADKKKVEDSIEEAIQ 585

Query: 481 WLERNQLAEVDEFEDKLKELEGLCNPIIAKLYQGGAGGDMPMGNGGGAGCSG-------- 540
           WL+ NQL E DEFEDK+KELE +CNPIIAK+YQGGAGG+      GG G SG        
Sbjct: 586 WLDGNQLGEADEFEDKMKELESVCNPIIAKMYQGGAGGE-----AGGPGASGMDEDEAPP 645

Query: 541 -GAGAGPKIEEVD 545
              GAGPKIEEVD
Sbjct: 646 ASGGAGPKIEEVD 653

BLAST of Lcy05g003590 vs. TAIR 10
Match: AT3G09440.1 (Heat shock protein 70 (Hsp 70) family protein )

HSP 1 Score: 882.9 bits (2280), Expect = 1.3e-256
Identity = 455/544 (83.64%), Postives = 489/544 (89.89%), Query Frame = 0

Query: 1   MIVVNYKGEEKQFAPEEISSMVLIKMKEIAEAYLGHNVKNAVITVPAYFNDSQRQATKDA 60
           MIVVNYKGE+K+F+ EEISSM+LIKM+EIAEAYLG  +KNAV+TVPAYFNDSQRQATKDA
Sbjct: 106 MIVVNYKGEDKEFSAEEISSMILIKMREIAEAYLGTTIKNAVVTVPAYFNDSQRQATKDA 165

Query: 61  GAIAGLNVLRIINEPTAAAIAYGLDKKASRSGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 120
           G IAGLNV+RIINEPTAAAIAYGLDKKA+  GEKNVLIFDLGGGTFDVSLLTIEEGIFEV
Sbjct: 166 GVIAGLNVMRIINEPTAAAIAYGLDKKATSVGEKNVLIFDLGGGTFDVSLLTIEEGIFEV 225

Query: 121 KATAGDTHLGGEDFDNRLVNHFAAEFKRKHKKDIGGNARALRRLRTACERAKRTLSSTTQ 180
           KATAGDTHLGGEDFDNR+VNHF  EFKRK+KKDI GN RALRRLRTACERAKRTLSST Q
Sbjct: 226 KATAGDTHLGGEDFDNRMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAKRTLSSTAQ 285

Query: 181 TTIEIDSLYEGIDFYATITRARFEELNMDLFRRCMEPVEKCLRDSKIDKGQIHDVVLVGG 240
           TTIEIDSL++GIDFYA ITRARFEELN+DLFR+CMEPVEKCLRD+K+DK  I DVVLVGG
Sbjct: 286 TTIEIDSLFDGIDFYAPITRARFEELNIDLFRKCMEPVEKCLRDAKMDKNSIDDVVLVGG 345

Query: 241 STRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILCGEGNEKVQDLLLLDVTPL 300
           STRIPKVQQLL DFFNGKELCKSINPDEAVAYGAAVQAAIL GEGNEKVQDLLLLDVTPL
Sbjct: 346 STRIPKVQQLLVDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGNEKVQDLLLLDVTPL 405

Query: 301 SLGIETAGGVMTVLIPRNTTIPTKKEQVFSTYSDNQTSVLIQVYEGERARTKDNNLLGTF 360
           SLG+ETAGGVMTVLI RNTTIPTKKEQVFSTYSDNQ  VLIQVYEGERARTKDNNLLG F
Sbjct: 406 SLGLETAGGVMTVLIQRNTTIPTKKEQVFSTYSDNQPGVLIQVYEGERARTKDNNLLGKF 465

Query: 361 ELKGIPPAPRGVPQINVCFDIDANGILNVTAEDKTAGVKNTITITNDKGRLSRDEIERMV 420
           EL GIPPAPRGVPQI VCFDIDANGILNV+AEDKT G KN ITITNDKGRLS+DEIE+MV
Sbjct: 466 ELSGIPPAPRGVPQITVCFDIDANGILNVSAEDKTTGQKNKITITNDKGRLSKDEIEKMV 525

Query: 421 QEAEKYKAEDEQVKKKVEAKNALENYAYNMRNTVRDEKLAGKMNPAEKGKIEKAIDEAID 480
           QEAEKYK+EDE+ KKKV+AKNALENYAYNMRNT+RDEK+  K+   +K KIE +I+ AI+
Sbjct: 526 QEAEKYKSEDEEHKKKVDAKNALENYAYNMRNTIRDEKIGEKLAGDDKKKIEDSIEAAIE 585

Query: 481 WLERNQLAEVDEFEDKLKELEGLCNPIIAKLYQGGAGGDMPMGNGGGAGCSGGAGAGPKI 540
           WLE NQLAE DEFEDK+KELE +CNPIIAK+YQGG  G    G           GAGPKI
Sbjct: 586 WLEANQLAECDEFEDKMKELESICNPIIAKMYQGGEAGGPAAGGMDEDVPPSAGGAGPKI 645

Query: 541 EEVD 545
           EEVD
Sbjct: 646 EEVD 649

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P264131.9e-26885.90Heat shock 70 kDa protein OS=Glycine max OX=3847 GN=HSP70 PE=3 SV=1[more]
Q9S9N12.2e-26485.32Heat shock 70 kDa protein 5 OS=Arabidopsis thaliana OX=3702 GN=HSP70-5 PE=2 SV=1[more]
P091891.1e-26385.71Heat shock cognate 70 kDa protein OS=Petunia hybrida OX=4102 GN=HSP70 PE=2 SV=1[more]
Q9LHA83.0e-26185.22Heat shock 70 kDa protein 4 OS=Arabidopsis thaliana OX=3702 GN=HSP70-4 PE=1 SV=1[more]
P273224.8e-25985.29Heat shock cognate 70 kDa protein 2 OS=Solanum lycopersicum OX=4081 GN=HSC-2 PE=... [more]
Match NameE-valueIdentityDescription
A0A6P3YWY04.6e-28192.48heat shock cognate 70 kDa protein-like OS=Ziziphus jujuba OX=326968 GN=LOC107405... [more]
A0A6J1BIS91.0e-28091.74heat shock 70 kDa protein 5 OS=Herrania umbratica OX=108875 GN=LOC110427096 PE=3... [more]
A0A6J1CSN48.7e-28094.53LOW QUALITY PROTEIN: heat shock 70 kDa protein OS=Momordica charantia OX=3673 GN... [more]
W9QV793.7e-27890.74Heat shock 70 kDa protein OS=Morus notabilis OX=981085 GN=L484_018081 PE=3 SV=1[more]
A0A059AJT04.8e-27890.93Uncharacterized protein OS=Eucalyptus grandis OX=71139 GN=EUGRSUZ_J03127 PE=3 SV... [more]
Match NameE-valueIdentityDescription
KAF3450992.17.3e-28191.32hypothetical protein FNV43_RR07081 [Rhamnella rubrinervis][more]
XP_015868485.19.6e-28192.48heat shock cognate 70 kDa protein-like [Ziziphus jujuba][more]
XP_021298179.12.1e-28091.74heat shock 70 kDa protein 5 [Herrania umbratica][more]
XP_022144559.11.8e-27994.53LOW QUALITY PROTEIN: heat shock 70 kDa protein [Momordica charantia][more]
XP_031273683.11.2e-27891.09heat shock 70 kDa protein-like [Pistacia vera][more]
Match NameE-valueIdentityDescription
AT1G16030.11.6e-26585.32heat shock protein 70B [more]
AT3G12580.12.1e-26285.22heat shock protein 70 [more]
AT5G02500.11.3e-25984.06heat shock cognate protein 70-1 [more]
AT5G02490.16.0e-25782.64Heat shock protein 70 (Hsp 70) family protein [more]
AT3G09440.11.3e-25683.64Heat shock protein 70 (Hsp 70) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 416..440
NoneNo IPR availableCOILSCoilCoilcoord: 483..503
NoneNo IPR availablePRINTSPR00301HEATSHOCK70coord: 291..310
score: 85.58
coord: 372..388
score: 76.88
coord: 264..284
score: 79.16
coord: 232..248
score: 79.69
coord: 41..61
score: 93.9
coord: 104..114
score: 79.84
NoneNo IPR availableGENE3D3.90.640.10Actin; Chain A, domain 4coord: 130..213
e-value: 6.3E-139
score: 464.8
NoneNo IPR availableGENE3D3.30.30.30coord: 2..21
e-value: 6.3E-139
score: 464.8
NoneNo IPR availableGENE3D3.30.420.40coord: 87..261
e-value: 6.3E-139
score: 464.8
NoneNo IPR availableGENE3D3.30.420.40coord: 22..278
e-value: 6.3E-139
score: 464.8
NoneNo IPR availablePANTHERPTHR19375:SF367SHOCK PROTEIN, PUTATIVE-RELATEDcoord: 1..522
IPR029048Heat shock protein 70kD, C-terminal domain superfamilyGENE3D1.20.1270.10coord: 440..544
e-value: 5.4E-40
score: 137.6
IPR029048Heat shock protein 70kD, C-terminal domain superfamilySUPERFAMILY100934Heat shock protein 70kD (HSP70), C-terminal subdomaincoord: 438..519
IPR029047Heat shock protein 70kD, peptide-binding domain superfamilyGENE3D2.60.34.10Substrate Binding Domain Of DNAk; Chain A, domain 1coord: 286..439
e-value: 1.1E-77
score: 261.2
IPR029047Heat shock protein 70kD, peptide-binding domain superfamilySUPERFAMILY100920Heat shock protein 70kD (HSP70), peptide-binding domaincoord: 287..444
IPR013126Heat shock protein 70 familyPFAMPF00012HSP70coord: 2..513
e-value: 5.1E-214
score: 711.9
IPR013126Heat shock protein 70 familyPANTHERPTHR19375HEAT SHOCK PROTEIN 70KDAcoord: 1..522
IPR018181Heat shock protein 70, conserved sitePROSITEPS01036HSP70_3coord: 235..249
IPR018181Heat shock protein 70, conserved sitePROSITEPS00329HSP70_2coord: 98..111
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 92..282
IPR043129ATPase, nucleotide binding domainSUPERFAMILY53067Actin-like ATPase domaincoord: 2..86

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy05g003590.1Lcy05g003590.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034620 cellular response to unfolded protein
biological_process GO:0051085 chaperone cofactor-dependent protein refolding
biological_process GO:0042026 protein refolding
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0031072 heat shock protein binding
molecular_function GO:0051787 misfolded protein binding
molecular_function GO:0044183 protein folding chaperone
molecular_function GO:0051082 unfolded protein binding