Lcy05g001040 (gene) Sponge gourd (P93075) v1

Overview
NameLcy05g001040
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionDNA-directed RNA polymerase I subunit rpa49-like
LocationChr05: 1046532 .. 1051880 (+)
RNA-Seq ExpressionLcy05g001040
SyntenyLcy05g001040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAACAAAGAGAAAACCCTAAACCCTTTCATCTCCTCTCACTCACAGTAACACTGCTCCTTCACGCCGGTTCCGCATCCCTTTCGGCAGCTCGGTGTCGCCGCCGCCGTCTTCGCCGTCCCCGCCGTCGTCTCTCTCCCATTCAGTCCCGCCGCCGCCTACCTCCTAGAGATTAGCACTGATTTTCCAGTCTCTTGTGGTCACTGGTATTCTCTTAATCTCCATCTACGTCTTTCTTCTTACCTGAAAACTTTAAATCAGTGAAATAAAGGAGTTGTTCTTTTTAATTTGTTGATTTGTTTGATTTGTTTGATCATTTTTATTTTATCACAATGGTAGAATAGCAACGAAATATAGTCACCCTCGTGGCAAATTGAGACGTTTCGAACTGTCTTGTATTAAACTCTGCCTTTTGATTTCTTGCACATGTCTGTCACTTGTCAATTGAGGCTTTGGTCATCTGTATACCTCTTTGGTCTATGCTCAAATTTGCTTCCGAAGCACCACACTCTGGTCATCTATATATCTAGAAGTATTCGAAGTAATCTATTATTATTATTATTTTAAATTTTTATATGCTATAATAAACGTTTTAAAACTGTAAATAGAAAAAATAAACTTAAAAAGTTTAGGTCTGTTTGAATTTTTGGTTTATGGTTTTTGAAAATTATGTTTGTTTTCTCATAATTTCATGATTTTCATCTTAGTTAAGTAGACGTTGGAATTTTTATCCAAACTCAGAAAACAAAACAAAATTTTAAAAACTATTTTATTTATTTATTTTTGTTAGTTTTGAAAACTTGACTTGAATTTTGGAAGACAGATAACAAAACAAAGGAATTCATATATGAAAGTAGTGTTAAGCTTAATTTTCAACCACCAAAAAACAGATACCAAATGGTTATCAAATTGGACCTTAAATAACAAATGAGGTTTAATGTCTTCATTTCTCAATTTCTCTCACAGTTTTTAAATGTTGTCGAGTTATAATTCATGTTGTATTTTCATATCTTGAAGGTATTCCAATAAAATACACAATGTCAATACAAATTTGAAAACCATCCCGAGCACAATTAGGCATTATAACTTTGGTCGTCTCAACTGATTGATTGAGACATTATAACCTCGACTAAAAAGTTAGATTTGTGAATCGTCCACTTGTTTAACTCAAGAAGTTACTAGAGGGATACATATAAAATTGTGATGACATAAGTGAGAGGACCATAAGTAAAAAAGTAAATTTTAGATTAATAATTGAGAGCAATACCAAACCCTAGCCTACTTTCCTCAATCTCTCTGTTCCACCTTCGTCTCCTCTCTCTAAAAATCAGCAGGTTTGATTATTTTCGCTTTCTACTTATTTCCGTGGAAGAATATGGTTTTAATTATGTATGAGCGTTCTTTGGCTATCTCTCTCTGTAGGCTCGAACAATGATAGGTTTTCTTTCCTTTCCTTTTTTAATGTCAAATTTTGGCGCTAATATGTGCTTGTGTGTCGTGTTCGTTGCTATTGTTGTTGTTTGCTTATGGGTGGTGTAATTGTTTCTGTAAATTACATGAATTTATTATTAAGTCTCAATCTATTGAATTAGTCATCTAGTTTTTTTGCCCGTTCCTATGGAACTGCGATTGTAATTAATTAGAGGAATTGTTGTTTATATTTCGTTTGTGGTTTTTTCCCGTGTAATTTTTATAGCCTCTGCTTTGGGAAAAGGTTAAAGTGGTGTCTGGTTCTAAAGATTTTGTTTTTCTCTTTATGAATGATGATATCAGATAGCCCTTGACACCTTTTCCTACATTGAAACAGAAGTTTAATGCTTGTTCTTCTCTTTATATGGACTGCAAGTTTGGCTTTTCTGTTACAGATTTCCTTTCTCACGATAGTTTCTTGGACAATTTCATTATGTTTTTCTGTCTATCCATTGTTCTCTCACGAGTCATGACCAATGAATGAATTGAGTTGATTAAATGATCACGATTTTGTTAGCCGTATAGATTGGTATATTTTAATTTGTTTATCTGTTCATCTTAATTTTCCCTTTCTTTAATAATCTCTTGCTTATATTTTGAACTATTTTCCCCTTGCAACCATGTCAGGTGTGGCTCCAGAAGGATTCCAGTTTTATTCTTTCCATTATGATGCAAAAAATGAATGAGATGTAATTCCCAGCCTGCTCTCAGAAAATTGACTTGTACGTTTTCTTTTTACAAACGAGCTGAAACTCCATGTGTTGATGATTGTTGGTGCATTGTAAAATCCGAGGTTTTCTTTGACTTCTACACACACTGAGGTTGTTAACGGAATTAATGGCGGAAGATTCTAGATCTTTTGAATTGGAAGAAACTCATTACACAGAACCAGAAGCAAATTCACAGTCTCCAGCGAAGAAGAAGAAGAGTGAGAAGAAGAAGAAGCGAGTTCCACCAGTGACTGCCAAAATCCAAGTCCTTAGCAACCATCCAGAGAAAACTCCGCCACTAGTTGGTTATTTCCCATCTGGGTTTGATCCTCACAAGGATGCTGATGAGGAAGCAGTTGAGGAGCAGAATTCTGTGAGGGCTAGGGTTTACAGGCACAAGAACAAACCAAATAGGATGCAGCTGGTTGTTAGTCCAACAGGGTCAAATGTGGATTTTGTTGGCACCAATTATTTGGGCGAGGCTACTGCTGGGAAACAGCATTGCACCTATGCTCTTGGTGTTCTTGATAAGGCGACCCAAAGCTTAAAGATTGTGCCTATTGCTTCAAATATGGTACTTTAGTCTTGATAATGAATGTAGAATATAAGAATTGTGGAAATTGGATCCTATTTGATGCTTGTTTGTTTTGGTCTAATCCCTAAAGAGACGTCTCTTATTCTTCTTCTTCATCTATTTATTTTTTTGTTTTCAAAATCTTAGTATGTTTTCCTTTGAAGGAATTTGGCTCCTTGATCTGCTGTGCTTGATTTTTCTCTGGAAAGACCATTGATTGATGAAGGTCTTTCAATATGAATTTTGAGCTGTTTTCATCTAGTTAAGAGAGCATAATTACTCAAATTTTGGGCTGATTATATTTTATGTATTTTAAAACTTGGTTGCACATATTTTTTTTTTTATTTTTTTGATATCCGTGAGTGTCCGGGCCAGCTTACGCGCACTTCGACTAATCTCATGGGACAACCGCCTGACCCTACTACATTTGGTTGCACATATTTTTTTCATTTTCATTTTGCTCAAAACTCACTGCAGAAGTTGCTAGAATTAGGCTTACCCTTTGAGATGCATTTTATCTACTTTACCAAATGTTCCATGCGCTGTAAAATTACTAAGCTTGATATTTATCCTTGCAGATATTTAGATTGGATCCAAAAGTCAGAGGTTCAGATGTTCCTGAAAAGGAATCCCCATGTGTGGCAAATGACGAACTTTCTGTGCAGGAGAAAGCTGATAGAATGAGAGAGCTGACCAATCTCTATGGAACAAAGAGGTCAATAAAGCAGGTAGAAACAGAATCCTGGAATTGCTTTCTGTTACTACTTTAGCAGTCATCTTTCATTTCCTTTTTAATGGTGGTAACTTATTTATTAAACAAATGCTGGACTCAACCTTATCAGCAGGTGAACAGAATTATGGGAGGCATGTTATCTTGAAAAAGGATTATATCTTTTCTCTTTACCTGTTTCATTGCTTTTAATTTGTTTTGGATGTGATCTGTTCCATTTATCCAATATCATTATTTTTATACTCTAATTGAAAAAGTATCTGATTTGAGATTTTCGATGTGTGACGACTGATAGTAAGTAAAAGAAACTTGGTTTTGGATGGAGTCGCTTCCAAATGGCAGTTTTAAAAAGTAGCAACAAATTGTTTGATATTGTTAATTTGTTGCAGGCAAAGAAGCTGCATTCTTTGAAGCAAGAGGATGATCCTGATACCAAGAAGGATCTGGATGTGAAAATGAAAAATGTAGTTGTAAATAAAGAGGCCTTGGAAAGTACAAATGCACAAATTGCTCGCAATATACCAACATATAATGCTTCTGCAACTACTCCACAAGAAGCTTACCCACTCGACAAGATTATTCTCAAGGGAGAATGGAGTTTCCTGGAAGATCTTTATTTCCTTTTGCAAGAGGAAGCTGAAATAAGTGCAAATTATCCATCCTTTGTCCGCAACAGAATTTATAAATTGCAAGACGTTCAGGTATGGTGTTAAGAAGGTTCTAATTGTCTTTATGAATATTCTGAGATAAAACATTCTGCCTTTCTGATACATATTCATGAATTGTGTAGATGGTCAACAGTATTTTTCGAGTATGATGAATCATGTTAATTCTTGGATCAAGCTATCTCGTCTTGTTTATTCGCTGATGTTTTCAATTCAAAATGACTGAATGCTTTCTGAAACTACTTGGCCTTTATGTTTCTGTGTGGAGTTTGATTCTTGGCCTTTATTTTTTGTGGAAAAGGAGCTTGCTCTTAAGTCTTAACAAGCATGCTTTATTTATTTCTTGATTCTTGTTCTAATTTGTATAGATTATTTATTTTTGACACAAGTCTTGTTTTCTATGTTGGAAACGAACTTGTTCTTAACCAAACAAGTATTATTTATTCAGGGTCTTTCACTTTTTGTTCATTTGTATTAAATCCTTTTTGATGTTAAAATCATCTTAGGACGAGGAAGAGAAGAAGAAGCTCTGCTGCATATTTTCATACATCACGCATCTAATTAAGTACAAGGACCAGCATTCCATGGACGGTGTTTCTTCTGCAAAGGGCCATAAAATCCCTAGCATGCTACGCCAAAAGTTCTCATCCATGTTTTCGACTACGGAGTCGAGAAGACTGTCAGCAGAGAAAACCGATCTTCTCATTAGCCACGTGTTGGTGCTTACGCTATTTGCCGATGAATTCAGCAGCGATCCATCAGACATAGCGAAGGATCTCAGAACTAGCCCTTTCACCCTAAGACTCCATTTTGATAACCTGGGTTGTAAGTTCATACGCAAGAACAACTTGTCATTGGTTACGCTTCCCATCCCGCTTCAATTTCCAGAGTTGAGGCAAAAGAGACGACGATGAGTGAGACGGTTAGATTGTAGTAGTTACACTGATTTTGTAATTTGAAGAAGCTGAATTCTACACAGGAAAGCTCGAGCAATTTTGTGGAGGAAAACGGGTAAATGGTCATATTGATAATTATTCATATGTTCTTTCTTTCATATTGCAAATGGCAACTTGTTTCTATGAAATGGCTCTGGCATAGAATGGTGTTTTCTTATTTGGAAGTTGGAACTTTGGATGCTGAAGCTTATTTGGAAATGAGTCAAAGGTTTATTCTTCATTTCTTCTTACAATAATCAACTTGTA

mRNA sequence

AAACAAAGAGAAAACCCTAAACCCTTTCATCTCCTCTCACTCACAGTAACACTGCTCCTTCACGCCGGTTCCGCATCCCTTTCGGCAGCTCGGTGTCGCCGCCGCCGTCTTCGCCGTCCCCGCCGTCGTCTCTCTCCCATTCAGTCCCGCCGCCGCCTACCTCCTAGAGATTAGCACTGATTTTCCAGTCTCTTGTGGTCACTGGTGTGGCTCCAGAAGGATTCCAGTTTTATTCTTTCCATTATGATGCAAAAAATGAATGAGATGTAATTCCCAGCCTGCTCTCAGAAAATTGACTTGTACGTTTTCTTTTTACAAACGAGCTGAAACTCCATGTGTTGATGATTGTTGGTGCATTGTAAAATCCGAGGTTTTCTTTGACTTCTACACACACTGAGGTTGTTAACGGAATTAATGGCGGAAGATTCTAGATCTTTTGAATTGGAAGAAACTCATTACACAGAACCAGAAGCAAATTCACAGTCTCCAGCGAAGAAGAAGAAGAGTGAGAAGAAGAAGAAGCGAGTTCCACCAGTGACTGCCAAAATCCAAGTCCTTAGCAACCATCCAGAGAAAACTCCGCCACTAGTTGGTTATTTCCCATCTGGGTTTGATCCTCACAAGGATGCTGATGAGGAAGCAGTTGAGGAGCAGAATTCTGTGAGGGCTAGGGTTTACAGGCACAAGAACAAACCAAATAGGATGCAGCTGGTTGTTAGTCCAACAGGGTCAAATGTGGATTTTGTTGGCACCAATTATTTGGGCGAGGCTACTGCTGGGAAACAGCATTGCACCTATGCTCTTGGTGTTCTTGATAAGGCGACCCAAAGCTTAAAGATTGTGCCTATTGCTTCAAATATGATATTTAGATTGGATCCAAAAGTCAGAGGTTCAGATGTTCCTGAAAAGGAATCCCCATGTGTGGCAAATGACGAACTTTCTGTGCAGGAGAAAGCTGATAGAATGAGAGAGCTGACCAATCTCTATGGAACAAAGAGGTCAATAAAGCAGGCAAAGAAGCTGCATTCTTTGAAGCAAGAGGATGATCCTGATACCAAGAAGGATCTGGATGTGAAAATGAAAAATGTAGTTGTAAATAAAGAGGCCTTGGAAAGTACAAATGCACAAATTGCTCGCAATATACCAACATATAATGCTTCTGCAACTACTCCACAAGAAGCTTACCCACTCGACAAGATTATTCTCAAGGGAGAATGGAGTTTCCTGGAAGATCTTTATTTCCTTTTGCAAGAGGAAGCTGAAATAAGTGCAAATTATCCATCCTTTGTCCGCAACAGAATTTATAAATTGCAAGACGTTCAGGACGAGGAAGAGAAGAAGAAGCTCTGCTGCATATTTTCATACATCACGCATCTAATTAAGTACAAGGACCAGCATTCCATGGACGGTGTTTCTTCTGCAAAGGGCCATAAAATCCCTAGCATGCTACGCCAAAAGTTCTCATCCATGTTTTCGACTACGGAGTCGAGAAGACTGTCAGCAGAGAAAACCGATCTTCTCATTAGCCACGTGTTGGTGCTTACGCTATTTGCCGATGAATTCAGCAGCGATCCATCAGACATAGCGAAGGATCTCAGAACTAGCCCTTTCACCCTAAGACTCCATTTTGATAACCTGGGTTGTAAGTTCATACGCAAGAACAACTTGTCATTGGTTACGCTTCCCATCCCGCTTCAATTTCCAGAGTTGAGGCAAAAGAGACGACGATGAGTGAGACGGTTAGATTGTAGTAGTTACACTGATTTTGTAATTTGAAGAAGCTGAATTCTACACAGGAAAGCTCGAGCAATTTTGTGGAGGAAAACGGGTAAATGGTCATATTGATAATTATTCATATGTTCTTTCTTTCATATTGCAAATGGCAACTTGTTTCTATGAAATGGCTCTGGCATAGAATGGTGTTTTCTTATTTGGAAGTTGGAACTTTGGATGCTGAAGCTTATTTGGAAATGAGTCAAAGGTTTATTCTTCATTTCTTCTTACAATAATCAACTTGTA

Coding sequence (CDS)

ATGGCGGAAGATTCTAGATCTTTTGAATTGGAAGAAACTCATTACACAGAACCAGAAGCAAATTCACAGTCTCCAGCGAAGAAGAAGAAGAGTGAGAAGAAGAAGAAGCGAGTTCCACCAGTGACTGCCAAAATCCAAGTCCTTAGCAACCATCCAGAGAAAACTCCGCCACTAGTTGGTTATTTCCCATCTGGGTTTGATCCTCACAAGGATGCTGATGAGGAAGCAGTTGAGGAGCAGAATTCTGTGAGGGCTAGGGTTTACAGGCACAAGAACAAACCAAATAGGATGCAGCTGGTTGTTAGTCCAACAGGGTCAAATGTGGATTTTGTTGGCACCAATTATTTGGGCGAGGCTACTGCTGGGAAACAGCATTGCACCTATGCTCTTGGTGTTCTTGATAAGGCGACCCAAAGCTTAAAGATTGTGCCTATTGCTTCAAATATGATATTTAGATTGGATCCAAAAGTCAGAGGTTCAGATGTTCCTGAAAAGGAATCCCCATGTGTGGCAAATGACGAACTTTCTGTGCAGGAGAAAGCTGATAGAATGAGAGAGCTGACCAATCTCTATGGAACAAAGAGGTCAATAAAGCAGGCAAAGAAGCTGCATTCTTTGAAGCAAGAGGATGATCCTGATACCAAGAAGGATCTGGATGTGAAAATGAAAAATGTAGTTGTAAATAAAGAGGCCTTGGAAAGTACAAATGCACAAATTGCTCGCAATATACCAACATATAATGCTTCTGCAACTACTCCACAAGAAGCTTACCCACTCGACAAGATTATTCTCAAGGGAGAATGGAGTTTCCTGGAAGATCTTTATTTCCTTTTGCAAGAGGAAGCTGAAATAAGTGCAAATTATCCATCCTTTGTCCGCAACAGAATTTATAAATTGCAAGACGTTCAGGACGAGGAAGAGAAGAAGAAGCTCTGCTGCATATTTTCATACATCACGCATCTAATTAAGTACAAGGACCAGCATTCCATGGACGGTGTTTCTTCTGCAAAGGGCCATAAAATCCCTAGCATGCTACGCCAAAAGTTCTCATCCATGTTTTCGACTACGGAGTCGAGAAGACTGTCAGCAGAGAAAACCGATCTTCTCATTAGCCACGTGTTGGTGCTTACGCTATTTGCCGATGAATTCAGCAGCGATCCATCAGACATAGCGAAGGATCTCAGAACTAGCCCTTTCACCCTAAGACTCCATTTTGATAACCTGGGTTGTAAGTTCATACGCAAGAACAACTTGTCATTGGTTACGCTTCCCATCCCGCTTCAATTTCCAGAGTTGAGGCAAAAGAGACGACGATGA

Protein sequence

MAEDSRSFELEETHYTEPEANSQSPAKKKKSEKKKKRVPPVTAKIQVLSNHPEKTPPLVGYFPSGFDPHKDADEEAVEEQNSVRARVYRHKNKPNRMQLVVSPTGSNVDFVGTNYLGEATAGKQHCTYALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVPEKESPCVANDELSVQEKADRMRELTNLYGTKRSIKQAKKLHSLKQEDDPDTKKDLDVKMKNVVVNKEALESTNAQIARNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFLLQEEAEISANYPSFVRNRIYKLQDVQDEEEKKKLCCIFSYITHLIKYKDQHSMDGVSSAKGHKIPSMLRQKFSSMFSTTESRRLSAEKTDLLISHVLVLTLFADEFSSDPSDIAKDLRTSPFTLRLHFDNLGCKFIRKNNLSLVTLPIPLQFPELRQKRRR
Homology
BLAST of Lcy05g001040 vs. ExPASy Swiss-Prot
Match: O14086 (DNA-directed RNA polymerase I subunit rpa49 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rpa49 PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 3.9e-09
Identity = 106/429 (24.71%), Postives = 171/429 (39.86%), Query Frame = 0

Query: 45  IQVLSNHPEKTPPLVGYFPSGFDPHKDADEEAVEEQNSVRARVYRHKNKPNRMQLVVSPT 104
           +++L       PPLV    SGF P             + R ++ +  N+ NR   ++  T
Sbjct: 24  VKILPLSESSLPPLVTTI-SGFYP-----------PENTRFQLLKKSNQSNRDASLIGRT 83

Query: 105 GSNVDFVGTNYLGEATAGKQHCTYALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVPE 164
              V F   N   ++TA  +   Y L V DK  +  KIVP      F  +       + E
Sbjct: 84  -ERVQFEAKN---QSTATVE-ANYCLAVADKTGRVKKIVPAKYLNTFERNILA----LQE 143

Query: 165 KESPCVANDELSVQEKADRMRELTNLYGTKRSIK--QAKKLHSLKQEDDPDTKKDLDVKM 224
           K+               ++   L   +GT++S K    +  + +K E   D K  L   +
Sbjct: 144 KDKFLKKKHGTVSGTVMEQRANLGLAFGTRKSQKAIMEESANRVKAETLGDVKDQL---V 203

Query: 225 KNVVVNKEALESTNAQIA------RNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYF 284
            NV    EAL  T   IA      R IP  N  A + ++AY L+ II K E+S +  +  
Sbjct: 204 SNVQKATEAL-PTQEDIAAAQAQDRPIPPVNVGAESIEDAYKLEDIIPKEEFSAIY-IKP 263

Query: 285 LLQEEAE------ISANYPSFVRNRIYKLQDVQDEEEKKKLCCIFSYITHLIKYKDQHSM 344
           LL+   E      +   +  F+  R  +L  +++ ++K+    I  YI+ L  +      
Sbjct: 264 LLENPDERNWAKLLPYRHSLFINERFQRLLSIEEVDQKR--ARILYYISLLQAFLFSRRS 323

Query: 345 DGVSSAKGHKI---PSMLRQKFSSMFSTTE---SRRLSAEKTDLLISHVLVLTLFADEFS 404
            G       K+   P +L       F+ T    S ++S+ + D +I ++LVL L  D +S
Sbjct: 324 VGNRETLRKKLADPPEILIDGLIKRFTQTTGIGSVQVSSREVDKIICYILVLCLIVDNYS 383

Query: 405 SDPSDIAKDLRTSPFTLRLHFDNLGCKFIR---------------KNNLSLVTLPIPLQF 439
           +D   +A DL          F  +GC+ +                  N     L IPL+F
Sbjct: 384 TDVLTLANDLNVKTMKANELFRTVGCRIMAYTETQRMALGLNKTDAKNHKRAVLKIPLEF 424

BLAST of Lcy05g001040 vs. ExPASy TrEMBL
Match: A0A6J1KIP6 (DNA-directed RNA polymerase I subunit rpa49-like OS=Cucurbita maxima OX=3661 GN=LOC111494318 PE=3 SV=1)

HSP 1 Score: 776.9 bits (2005), Expect = 4.3e-221
Identity = 397/438 (90.64%), Postives = 417/438 (95.21%), Query Frame = 0

Query: 1   MAEDSRSFELEETHYTEPEANSQSPAKKKKSEKKKKRVPPVTAKIQVLSNHPEKTPPLVG 60
           MA DS+ F  EETHY  PEANSQSP KKKKSEKKKKRVPPV  KIQVLSNHPEK PPLVG
Sbjct: 1   MAGDSKYF--EETHYAVPEANSQSPVKKKKSEKKKKRVPPVNTKIQVLSNHPEKIPPLVG 60

Query: 61  YFPSGFDPHKDADEEAVEEQNSVRARVYRHKNKPNRMQLVVSPTGSNVDFVGTNYLGEAT 120
           YFPSG+DP+KD +EEAVEEQNSVRA+VYRHK KPNRMQL+VSPTGSNVDFVGTNYLGEAT
Sbjct: 61  YFPSGYDPNKDPNEEAVEEQNSVRAKVYRHKKKPNRMQLIVSPTGSNVDFVGTNYLGEAT 120

Query: 121 AGKQHCTYALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVPEKESPCVANDELSVQEK 180
           AGKQHC+YALGVLDKATQSLKIVPIASNMIFRLDPKV GSDVPE ESP VANDELSVQEK
Sbjct: 121 AGKQHCSYALGVLDKATQSLKIVPIASNMIFRLDPKVSGSDVPENESPSVANDELSVQEK 180

Query: 181 ADRMRELTNLYGTKRSIKQAKKLHSLKQEDDPDTKKDLDVKMKNVVVNKEALESTNAQIA 240
           ADRMREL+NLYGTK+SIKQAKKL SLKQEDDP+TKKDLD KMKNVVVNKEALES+NAQI+
Sbjct: 181 ADRMRELSNLYGTKKSIKQAKKLLSLKQEDDPETKKDLDGKMKNVVVNKEALESSNAQIS 240

Query: 241 RNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFLLQEEAEISANYPSFVRNRIYKLQ 300
           RNIPTYNASATTPQEAYPLDKIILKGEW FLEDLYFLLQ E+EISANYPSFVRNRIYKLQ
Sbjct: 241 RNIPTYNASATTPQEAYPLDKIILKGEWGFLEDLYFLLQAESEISANYPSFVRNRIYKLQ 300

Query: 301 DVQDEEEKKKLCCIFSYITHLIKYKDQHSMDGVSSAKGHKIPSMLRQKFSSMFSTTESRR 360
           D+QDEEEKKKLCCI+SYITHLIKYKDQHSMDGVSS +GHKIPSMLRQKFSSMFSTTESRR
Sbjct: 301 DIQDEEEKKKLCCIYSYITHLIKYKDQHSMDGVSSVEGHKIPSMLRQKFSSMFSTTESRR 360

Query: 361 LSAEKTDLLISHVLVLTLFADEFSSDPSDIAKDLRTSPFTLRLHFDNLGCKFIRKNNLSL 420
           LSAEKTDLLISHVLVLTLFADEFSSDPSDIA+DLRTSPFTLR+H+DNLGCKFIRKNNLS 
Sbjct: 361 LSAEKTDLLISHVLVLTLFADEFSSDPSDIARDLRTSPFTLRVHYDNLGCKFIRKNNLSW 420

Query: 421 VTLPIPLQFPELRQKRRR 439
           VTLP+PLQFPELRQKRRR
Sbjct: 421 VTLPVPLQFPELRQKRRR 436

BLAST of Lcy05g001040 vs. ExPASy TrEMBL
Match: A0A6J1HM51 (DNA-directed RNA polymerase I subunit rpa49-like OS=Cucurbita moschata OX=3662 GN=LOC111464231 PE=3 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 7.4e-221
Identity = 399/439 (90.89%), Postives = 418/439 (95.22%), Query Frame = 0

Query: 1   MAEDSRSFELEETHYTEPEANSQSPA-KKKKSEKKKKRVPPVTAKIQVLSNHPEKTPPLV 60
           MA DS+ F  EETHY EPEANSQSPA KKKKSEKKKKRVPPV  KIQVLSNHPEK PPLV
Sbjct: 1   MAGDSKYF--EETHYAEPEANSQSPAKKKKKSEKKKKRVPPVNTKIQVLSNHPEKIPPLV 60

Query: 61  GYFPSGFDPHKDADEEAVEEQNSVRARVYRHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120
           GYFPSG+DP+KD +EE VEEQNSVRA+VYRHK KPNRMQLVVSPTGSNVDFVGTNYLGEA
Sbjct: 61  GYFPSGYDPNKDPNEEVVEEQNSVRAKVYRHKKKPNRMQLVVSPTGSNVDFVGTNYLGEA 120

Query: 121 TAGKQHCTYALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVPEKESPCVANDELSVQE 180
           TAGKQHC+YALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVPE ESP VANDELSVQE
Sbjct: 121 TAGKQHCSYALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVPENESPSVANDELSVQE 180

Query: 181 KADRMRELTNLYGTKRSIKQAKKLHSLKQEDDPDTKKDLDVKMKNVVVNKEALESTNAQI 240
           KADRMREL+NLYGTK+SIKQAKKL SLKQEDDP+TKKDLD KMKNVVVNKEALES+NAQI
Sbjct: 181 KADRMRELSNLYGTKKSIKQAKKLLSLKQEDDPETKKDLDGKMKNVVVNKEALESSNAQI 240

Query: 241 ARNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFLLQEEAEISANYPSFVRNRIYKL 300
           +RNIPTYNASATTPQEAYPLDKIILKGEW FLEDLYFLLQ E+EISANYPSFVRNRIYKL
Sbjct: 241 SRNIPTYNASATTPQEAYPLDKIILKGEWGFLEDLYFLLQAESEISANYPSFVRNRIYKL 300

Query: 301 QDVQDEEEKKKLCCIFSYITHLIKYKDQHSMDGVSSAKGHKIPSMLRQKFSSMFSTTESR 360
           QD+QDEEEKKKLCCI+SYITHLIKYKDQHSMDGVSS KGHKIPSMLRQKFSSMFSTTESR
Sbjct: 301 QDIQDEEEKKKLCCIYSYITHLIKYKDQHSMDGVSSVKGHKIPSMLRQKFSSMFSTTESR 360

Query: 361 RLSAEKTDLLISHVLVLTLFADEFSSDPSDIAKDLRTSPFTLRLHFDNLGCKFIRKNNLS 420
           RLSAEKTDLLISHVLVL LF DEFSSDPSDIA+DLRTSPFTLR+H+DNLGCKFIRKNNLS
Sbjct: 361 RLSAEKTDLLISHVLVLALFPDEFSSDPSDIARDLRTSPFTLRVHYDNLGCKFIRKNNLS 420

Query: 421 LVTLPIPLQFPELRQKRRR 439
           LVTLP+PLQFPELR+KRRR
Sbjct: 421 LVTLPVPLQFPELRRKRRR 437

BLAST of Lcy05g001040 vs. ExPASy TrEMBL
Match: A0A6J1DBN9 (DNA-directed RNA polymerase I subunit rpa49 OS=Momordica charantia OX=3673 GN=LOC111019522 PE=3 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 5.9e-218
Identity = 395/439 (89.98%), Postives = 416/439 (94.76%), Query Frame = 0

Query: 1   MAEDSRSFELEETHYTEPEANSQSPAKK-KKSEKKKKRVPPVTAKIQVLSNHPEKTPPLV 60
           MA D++SFELEETHY EPEANSQSPAKK +KSEKKKKRV  V A IQVLSNHPEKTPPLV
Sbjct: 1   MAADTKSFELEETHYAEPEANSQSPAKKNRKSEKKKKRVSSVNATIQVLSNHPEKTPPLV 60

Query: 61  GYFPSGFDPHKDADEEAVEEQNSVRARVYRHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120
           GYFPSGF+PHKDADEEAV+EQ S+RARVYR+KNKPNRMQLVVSPTGSNVDFVGTNYLGEA
Sbjct: 61  GYFPSGFNPHKDADEEAVQEQKSIRARVYRNKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120

Query: 121 TAGKQHCTYALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVPEKESPCVANDELSVQE 180
           TAGKQHCTYALGVLDKATQSLKIVPIA+NMIFRLDPKVRGSDV EKESP V +DELSVQE
Sbjct: 121 TAGKQHCTYALGVLDKATQSLKIVPIAANMIFRLDPKVRGSDVSEKESPTVVDDELSVQE 180

Query: 181 KADRMRELTNLYGTKRSIKQAKKLHSLKQEDDPDTKKDLDVKMKNVVVNKEALESTNAQI 240
           KADRMRELTNLYGTKRSIKQAKKLHSLKQ DD DT+KDLD KMKNVVVNKEALE TNA+I
Sbjct: 181 KADRMRELTNLYGTKRSIKQAKKLHSLKQVDDSDTQKDLDEKMKNVVVNKEALEGTNAEI 240

Query: 241 ARNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFLLQEEAEISANYPSFVRNRIYKL 300
           ARNIPTYNASATTPQEAYPLDKIIL GEWS+LED+Y LL+ EAEISANYPSFVRNRIYKL
Sbjct: 241 ARNIPTYNASATTPQEAYPLDKIILMGEWSYLEDVYSLLRAEAEISANYPSFVRNRIYKL 300

Query: 301 QDVQDEEEKKKLCCIFSYITHLIKYKDQHSMDGVSSAKGHKIPSMLRQKFSSMFSTTESR 360
           QD+QDEEEKKKLCCIFSYITHLIK+KDQ+SMDGVSSAKGHKIPSMLRQKFSSMFS  ESR
Sbjct: 301 QDIQDEEEKKKLCCIFSYITHLIKFKDQNSMDGVSSAKGHKIPSMLRQKFSSMFSLLESR 360

Query: 361 RLSAEKTDLLISHVLVLTLFADEFSSDPSDIAKDLRTSPFTLRLHFDNLGCKFIRKNNLS 420
           RLS EK+DLLISHVLVLTLFADEFSSDPSDIAKDLRTSPFTLRLHFDNLGCKFIRKNNLS
Sbjct: 361 RLSPEKSDLLISHVLVLTLFADEFSSDPSDIAKDLRTSPFTLRLHFDNLGCKFIRKNNLS 420

Query: 421 LVTLPIPLQFPELRQKRRR 439
           LVTLP+PL+FP+L  KRRR
Sbjct: 421 LVTLPVPLKFPQLSLKRRR 439

BLAST of Lcy05g001040 vs. ExPASy TrEMBL
Match: A0A6J1JX25 (DNA-directed RNA polymerase I subunit rpa49-like OS=Cucurbita maxima OX=3661 GN=LOC111488633 PE=3 SV=1)

HSP 1 Score: 751.5 bits (1939), Expect = 2.0e-213
Identity = 386/439 (87.93%), Postives = 411/439 (93.62%), Query Frame = 0

Query: 1   MAEDSRSFELEETHYTEPEANSQSPA-KKKKSEKKKKRVPPVTAKIQVLSNHPEKTPPLV 60
           MAED++SFELE+ HY+EP+ANSQSPA KKKKSEKKKKRVP V AKIQVLSNHP+KTPPLV
Sbjct: 1   MAEDTKSFELEDNHYSEPDANSQSPAKKKKKSEKKKKRVPSVNAKIQVLSNHPQKTPPLV 60

Query: 61  GYFPSGFDPHKDADEEAVEEQNSVRARVYRHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120
            YFPSGFDPHKDA+EEAVEEQ+SVRARVYRHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA
Sbjct: 61  CYFPSGFDPHKDANEEAVEEQDSVRARVYRHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120

Query: 121 TAGKQHCTYALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVPEKESPCVANDELSVQE 180
           TAGKQHC+Y LGVLDKATQSLKIVPIASNMIFRLDP++RGSDV E ES  V N+ELSVQE
Sbjct: 121 TAGKQHCSYGLGVLDKATQSLKIVPIASNMIFRLDPRIRGSDVSEMESQSVENEELSVQE 180

Query: 181 KADRMRELTNLYGTKRSIKQAKKLHSLKQEDDPDTKKDLDVKMKNVVVNKEALESTNAQI 240
           KA++MRELTNLYGTK+SI QAKKLHSLKQEDDPDTKKDLD KMKNVVVNKEALEST AQI
Sbjct: 181 KAEKMRELTNLYGTKKSINQAKKLHSLKQEDDPDTKKDLDGKMKNVVVNKEALESTGAQI 240

Query: 241 ARNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFLLQEEAEISANYPSFVRNRIYKL 300
           ARNIPTYN SATTP+EAYPLDKIILKGEWSFLED+Y LLQEEAEISA YPSFVRNRIYKL
Sbjct: 241 ARNIPTYNVSATTPREAYPLDKIILKGEWSFLEDIYSLLQEEAEISAKYPSFVRNRIYKL 300

Query: 301 QDVQDEEEKKKLCCIFSYITHLIKYKDQHSMDGVSSAKGHKIPSMLRQKFSSMFSTTESR 360
           QDVQDEEEKK+LC IFSYITHLIKYKDQHSM  V+SAK HK PSM+RQKFSSMF T+E+R
Sbjct: 301 QDVQDEEEKKQLCSIFSYITHLIKYKDQHSMGDVASAKSHKFPSMIRQKFSSMFPTSEAR 360

Query: 361 RLSAEKTDLLISHVLVLTLFADEFSSDPSDIAKDLRTSPFTLRLHFDNLGCKFIRKNNLS 420
           RLS E  +LLISHVLVLTLFADEFSSDPSDIAKDLRTSP+ LRLHFDNLGCKFIRKNNLS
Sbjct: 361 RLSTETINLLISHVLVLTLFADEFSSDPSDIAKDLRTSPYDLRLHFDNLGCKFIRKNNLS 420

Query: 421 LVTLPIPLQFPELRQKRRR 439
           LVTLP PL+FPELRQKRRR
Sbjct: 421 LVTLPTPLEFPELRQKRRR 439

BLAST of Lcy05g001040 vs. ExPASy TrEMBL
Match: A0A6J1GQS4 (DNA-directed RNA polymerase I subunit rpa49-like OS=Cucurbita moschata OX=3662 GN=LOC111456250 PE=3 SV=1)

HSP 1 Score: 746.1 bits (1925), Expect = 8.2e-212
Identity = 383/439 (87.24%), Postives = 409/439 (93.17%), Query Frame = 0

Query: 1   MAEDSRSFELEETHYTEPEANSQSPA-KKKKSEKKKKRVPPVTAKIQVLSNHPEKTPPLV 60
           MAED++SFELE+ HY+EP++N QSPA KKKKSEKKKKRVP V AKIQVLSNHP+KTPPLV
Sbjct: 1   MAEDTKSFELEDNHYSEPDSNLQSPAKKKKKSEKKKKRVPSVNAKIQVLSNHPQKTPPLV 60

Query: 61  GYFPSGFDPHKDADEEAVEEQNSVRARVYRHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120
            YFPSGFDPHKD +EEAVEEQ+SVRARVYRHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA
Sbjct: 61  CYFPSGFDPHKDVNEEAVEEQDSVRARVYRHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120

Query: 121 TAGKQHCTYALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVPEKESPCVANDELSVQE 180
           TAGKQHC+Y LGVLDKATQSLKIVPIASNMIFRLDP++RGSDV E ESP V N+ELSVQE
Sbjct: 121 TAGKQHCSYGLGVLDKATQSLKIVPIASNMIFRLDPRIRGSDVSEMESPSVENEELSVQE 180

Query: 181 KADRMRELTNLYGTKRSIKQAKKLHSLKQEDDPDTKKDLDVKMKNVVVNKEALESTNAQI 240
           KA++MRELTNLYGTK+SI QAKKL SLKQEDDPDTKKDLD KMKNVVVNKEALEST AQI
Sbjct: 181 KAEKMRELTNLYGTKKSINQAKKLRSLKQEDDPDTKKDLDGKMKNVVVNKEALESTGAQI 240

Query: 241 ARNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFLLQEEAEISANYPSFVRNRIYKL 300
           ARNIPTYN SATTP+EAYPLDKIILKGEWS+LEDLY LLQEEAEISA YPSFVRNRIYKL
Sbjct: 241 ARNIPTYNVSATTPREAYPLDKIILKGEWSYLEDLYSLLQEEAEISAKYPSFVRNRIYKL 300

Query: 301 QDVQDEEEKKKLCCIFSYITHLIKYKDQHSMDGVSSAKGHKIPSMLRQKFSSMFSTTESR 360
           QDVQDEEEKK+LC IFSYITHLIKYKDQHSM  V+SAK HK PSM+RQKFSSMF T+E+R
Sbjct: 301 QDVQDEEEKKQLCSIFSYITHLIKYKDQHSMGDVASAKSHKFPSMIRQKFSSMFPTSEAR 360

Query: 361 RLSAEKTDLLISHVLVLTLFADEFSSDPSDIAKDLRTSPFTLRLHFDNLGCKFIRKNNLS 420
           RLS E  +LLISHVLVLTLFADEFSSDPSDIAKDLRTSP+ LRLHFDNLGCKFIRKNNLS
Sbjct: 361 RLSTETINLLISHVLVLTLFADEFSSDPSDIAKDLRTSPYDLRLHFDNLGCKFIRKNNLS 420

Query: 421 LVTLPIPLQFPELRQKRRR 439
           LVTLP PL+FPELRQKRRR
Sbjct: 421 LVTLPTPLEFPELRQKRRR 439

BLAST of Lcy05g001040 vs. NCBI nr
Match: KAG7025877.1 (DNA-directed RNA polymerase I subunit rpa49 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 780.0 bits (2013), Expect = 1.1e-221
Identity = 398/438 (90.87%), Postives = 418/438 (95.43%), Query Frame = 0

Query: 1   MAEDSRSFELEETHYTEPEANSQSPAKKKKSEKKKKRVPPVTAKIQVLSNHPEKTPPLVG 60
           MA DS+ F  EETHY EPEANSQ PAKKKKSEKKKKRVPPV  KIQVLSNHPEK PPLVG
Sbjct: 1   MAGDSKYF--EETHYAEPEANSQFPAKKKKSEKKKKRVPPVNTKIQVLSNHPEKIPPLVG 60

Query: 61  YFPSGFDPHKDADEEAVEEQNSVRARVYRHKNKPNRMQLVVSPTGSNVDFVGTNYLGEAT 120
           YFPSG+DP+KD +EE VEEQNSVRA+VYRHK KPNRMQLVVSPTGSNVDFVGTNYLGEAT
Sbjct: 61  YFPSGYDPNKDPNEEVVEEQNSVRAKVYRHKKKPNRMQLVVSPTGSNVDFVGTNYLGEAT 120

Query: 121 AGKQHCTYALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVPEKESPCVANDELSVQEK 180
           AGKQHC+YALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVPE ESP VANDELSVQEK
Sbjct: 121 AGKQHCSYALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVPENESPSVANDELSVQEK 180

Query: 181 ADRMRELTNLYGTKRSIKQAKKLHSLKQEDDPDTKKDLDVKMKNVVVNKEALESTNAQIA 240
           ADRMREL+NLYGTK+SIKQAKKL SLKQEDDP+TKKDLD KMKNVVVNKEALES+NAQI+
Sbjct: 181 ADRMRELSNLYGTKKSIKQAKKLLSLKQEDDPETKKDLDGKMKNVVVNKEALESSNAQIS 240

Query: 241 RNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFLLQEEAEISANYPSFVRNRIYKLQ 300
           RNIPTYNASATTPQEAYPLDKIILKGEW FLEDLYFLLQ E+EISANYPSFVRNRIYKLQ
Sbjct: 241 RNIPTYNASATTPQEAYPLDKIILKGEWGFLEDLYFLLQAESEISANYPSFVRNRIYKLQ 300

Query: 301 DVQDEEEKKKLCCIFSYITHLIKYKDQHSMDGVSSAKGHKIPSMLRQKFSSMFSTTESRR 360
           D+QDEEEKKKLCCI+SYITHL+KYKDQHSMDGVSS KGHKIPSMLRQKFSSMFSTTESRR
Sbjct: 301 DIQDEEEKKKLCCIYSYITHLVKYKDQHSMDGVSSVKGHKIPSMLRQKFSSMFSTTESRR 360

Query: 361 LSAEKTDLLISHVLVLTLFADEFSSDPSDIAKDLRTSPFTLRLHFDNLGCKFIRKNNLSL 420
           LSAEKTDLLISHVLVL LFADEFSSDPSDIA+DLRTSPFTLR+H+DNLGCKFIRKNNLSL
Sbjct: 361 LSAEKTDLLISHVLVLALFADEFSSDPSDIARDLRTSPFTLRVHYDNLGCKFIRKNNLSL 420

Query: 421 VTLPIPLQFPELRQKRRR 439
           VTLP+PLQFPELR+KRRR
Sbjct: 421 VTLPVPLQFPELRRKRRR 436

BLAST of Lcy05g001040 vs. NCBI nr
Match: XP_023514670.1 (DNA-directed RNA polymerase I subunit rpa49-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 777.7 bits (2007), Expect = 5.3e-221
Identity = 400/439 (91.12%), Postives = 419/439 (95.44%), Query Frame = 0

Query: 1   MAEDSRSFELEETHYTEPEANSQSPA-KKKKSEKKKKRVPPVTAKIQVLSNHPEKTPPLV 60
           MA DS+ F  EETHY EPEANSQSPA KKKKSEKKKKRVPPV  KIQVLSNHPEK PPLV
Sbjct: 5   MAGDSKYF--EETHYAEPEANSQSPAKKKKKSEKKKKRVPPVNTKIQVLSNHPEKIPPLV 64

Query: 61  GYFPSGFDPHKDADEEAVEEQNSVRARVYRHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120
           GYFPSG+DP+KD +EEAVEEQNSVRA+VYRHK KPNRMQLVVSPTG NVDFVGTNYLGEA
Sbjct: 65  GYFPSGYDPNKDPNEEAVEEQNSVRAKVYRHKKKPNRMQLVVSPTGLNVDFVGTNYLGEA 124

Query: 121 TAGKQHCTYALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVPEKESPCVANDELSVQE 180
           TAGKQHC+YALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVPE ESP VANDELSVQE
Sbjct: 125 TAGKQHCSYALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVPESESPSVANDELSVQE 184

Query: 181 KADRMRELTNLYGTKRSIKQAKKLHSLKQEDDPDTKKDLDVKMKNVVVNKEALESTNAQI 240
           KADRMREL+NLYGTK+SIKQAKKL SLKQEDDP+TKKDLD KMKNVVVNKEALES+NAQI
Sbjct: 185 KADRMRELSNLYGTKKSIKQAKKLLSLKQEDDPETKKDLDGKMKNVVVNKEALESSNAQI 244

Query: 241 ARNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFLLQEEAEISANYPSFVRNRIYKL 300
           +RNIPTYNASATTPQEAYPLDKIILKGEW FLEDLYFLLQ E+EISANYP+FVRNRIYKL
Sbjct: 245 SRNIPTYNASATTPQEAYPLDKIILKGEWGFLEDLYFLLQAESEISANYPAFVRNRIYKL 304

Query: 301 QDVQDEEEKKKLCCIFSYITHLIKYKDQHSMDGVSSAKGHKIPSMLRQKFSSMFSTTESR 360
           QD+QDEEEKKKLCCI+SYITHLIKYKDQHSMDGVSS KGHKIPSMLRQKFSSMFSTTESR
Sbjct: 305 QDIQDEEEKKKLCCIYSYITHLIKYKDQHSMDGVSSVKGHKIPSMLRQKFSSMFSTTESR 364

Query: 361 RLSAEKTDLLISHVLVLTLFADEFSSDPSDIAKDLRTSPFTLRLHFDNLGCKFIRKNNLS 420
           RLSAEKTDLLISHVLVL LFADEFSSDPSDIA+DLRTSPFTLR+H+DNLGCKFIRKNNLS
Sbjct: 365 RLSAEKTDLLISHVLVLALFADEFSSDPSDIARDLRTSPFTLRVHYDNLGCKFIRKNNLS 424

Query: 421 LVTLPIPLQFPELRQKRRR 439
           LVTLP+PLQFPELRQKRRR
Sbjct: 425 LVTLPVPLQFPELRQKRRR 441

BLAST of Lcy05g001040 vs. NCBI nr
Match: XP_023000003.1 (DNA-directed RNA polymerase I subunit rpa49-like [Cucurbita maxima] >XP_023000004.1 DNA-directed RNA polymerase I subunit rpa49-like [Cucurbita maxima] >XP_023000005.1 DNA-directed RNA polymerase I subunit rpa49-like [Cucurbita maxima] >XP_023000006.1 DNA-directed RNA polymerase I subunit rpa49-like [Cucurbita maxima])

HSP 1 Score: 776.9 bits (2005), Expect = 9.0e-221
Identity = 397/438 (90.64%), Postives = 417/438 (95.21%), Query Frame = 0

Query: 1   MAEDSRSFELEETHYTEPEANSQSPAKKKKSEKKKKRVPPVTAKIQVLSNHPEKTPPLVG 60
           MA DS+ F  EETHY  PEANSQSP KKKKSEKKKKRVPPV  KIQVLSNHPEK PPLVG
Sbjct: 1   MAGDSKYF--EETHYAVPEANSQSPVKKKKSEKKKKRVPPVNTKIQVLSNHPEKIPPLVG 60

Query: 61  YFPSGFDPHKDADEEAVEEQNSVRARVYRHKNKPNRMQLVVSPTGSNVDFVGTNYLGEAT 120
           YFPSG+DP+KD +EEAVEEQNSVRA+VYRHK KPNRMQL+VSPTGSNVDFVGTNYLGEAT
Sbjct: 61  YFPSGYDPNKDPNEEAVEEQNSVRAKVYRHKKKPNRMQLIVSPTGSNVDFVGTNYLGEAT 120

Query: 121 AGKQHCTYALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVPEKESPCVANDELSVQEK 180
           AGKQHC+YALGVLDKATQSLKIVPIASNMIFRLDPKV GSDVPE ESP VANDELSVQEK
Sbjct: 121 AGKQHCSYALGVLDKATQSLKIVPIASNMIFRLDPKVSGSDVPENESPSVANDELSVQEK 180

Query: 181 ADRMRELTNLYGTKRSIKQAKKLHSLKQEDDPDTKKDLDVKMKNVVVNKEALESTNAQIA 240
           ADRMREL+NLYGTK+SIKQAKKL SLKQEDDP+TKKDLD KMKNVVVNKEALES+NAQI+
Sbjct: 181 ADRMRELSNLYGTKKSIKQAKKLLSLKQEDDPETKKDLDGKMKNVVVNKEALESSNAQIS 240

Query: 241 RNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFLLQEEAEISANYPSFVRNRIYKLQ 300
           RNIPTYNASATTPQEAYPLDKIILKGEW FLEDLYFLLQ E+EISANYPSFVRNRIYKLQ
Sbjct: 241 RNIPTYNASATTPQEAYPLDKIILKGEWGFLEDLYFLLQAESEISANYPSFVRNRIYKLQ 300

Query: 301 DVQDEEEKKKLCCIFSYITHLIKYKDQHSMDGVSSAKGHKIPSMLRQKFSSMFSTTESRR 360
           D+QDEEEKKKLCCI+SYITHLIKYKDQHSMDGVSS +GHKIPSMLRQKFSSMFSTTESRR
Sbjct: 301 DIQDEEEKKKLCCIYSYITHLIKYKDQHSMDGVSSVEGHKIPSMLRQKFSSMFSTTESRR 360

Query: 361 LSAEKTDLLISHVLVLTLFADEFSSDPSDIAKDLRTSPFTLRLHFDNLGCKFIRKNNLSL 420
           LSAEKTDLLISHVLVLTLFADEFSSDPSDIA+DLRTSPFTLR+H+DNLGCKFIRKNNLS 
Sbjct: 361 LSAEKTDLLISHVLVLTLFADEFSSDPSDIARDLRTSPFTLRVHYDNLGCKFIRKNNLSW 420

Query: 421 VTLPIPLQFPELRQKRRR 439
           VTLP+PLQFPELRQKRRR
Sbjct: 421 VTLPVPLQFPELRQKRRR 436

BLAST of Lcy05g001040 vs. NCBI nr
Match: XP_022964095.1 (DNA-directed RNA polymerase I subunit rpa49-like [Cucurbita moschata] >XP_022964096.1 DNA-directed RNA polymerase I subunit rpa49-like [Cucurbita moschata] >XP_022964097.1 DNA-directed RNA polymerase I subunit rpa49-like [Cucurbita moschata] >XP_022964098.1 DNA-directed RNA polymerase I subunit rpa49-like [Cucurbita moschata] >XP_022964099.1 DNA-directed RNA polymerase I subunit rpa49-like [Cucurbita moschata])

HSP 1 Score: 776.2 bits (2003), Expect = 1.5e-220
Identity = 399/439 (90.89%), Postives = 418/439 (95.22%), Query Frame = 0

Query: 1   MAEDSRSFELEETHYTEPEANSQSPA-KKKKSEKKKKRVPPVTAKIQVLSNHPEKTPPLV 60
           MA DS+ F  EETHY EPEANSQSPA KKKKSEKKKKRVPPV  KIQVLSNHPEK PPLV
Sbjct: 1   MAGDSKYF--EETHYAEPEANSQSPAKKKKKSEKKKKRVPPVNTKIQVLSNHPEKIPPLV 60

Query: 61  GYFPSGFDPHKDADEEAVEEQNSVRARVYRHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120
           GYFPSG+DP+KD +EE VEEQNSVRA+VYRHK KPNRMQLVVSPTGSNVDFVGTNYLGEA
Sbjct: 61  GYFPSGYDPNKDPNEEVVEEQNSVRAKVYRHKKKPNRMQLVVSPTGSNVDFVGTNYLGEA 120

Query: 121 TAGKQHCTYALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVPEKESPCVANDELSVQE 180
           TAGKQHC+YALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVPE ESP VANDELSVQE
Sbjct: 121 TAGKQHCSYALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVPENESPSVANDELSVQE 180

Query: 181 KADRMRELTNLYGTKRSIKQAKKLHSLKQEDDPDTKKDLDVKMKNVVVNKEALESTNAQI 240
           KADRMREL+NLYGTK+SIKQAKKL SLKQEDDP+TKKDLD KMKNVVVNKEALES+NAQI
Sbjct: 181 KADRMRELSNLYGTKKSIKQAKKLLSLKQEDDPETKKDLDGKMKNVVVNKEALESSNAQI 240

Query: 241 ARNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFLLQEEAEISANYPSFVRNRIYKL 300
           +RNIPTYNASATTPQEAYPLDKIILKGEW FLEDLYFLLQ E+EISANYPSFVRNRIYKL
Sbjct: 241 SRNIPTYNASATTPQEAYPLDKIILKGEWGFLEDLYFLLQAESEISANYPSFVRNRIYKL 300

Query: 301 QDVQDEEEKKKLCCIFSYITHLIKYKDQHSMDGVSSAKGHKIPSMLRQKFSSMFSTTESR 360
           QD+QDEEEKKKLCCI+SYITHLIKYKDQHSMDGVSS KGHKIPSMLRQKFSSMFSTTESR
Sbjct: 301 QDIQDEEEKKKLCCIYSYITHLIKYKDQHSMDGVSSVKGHKIPSMLRQKFSSMFSTTESR 360

Query: 361 RLSAEKTDLLISHVLVLTLFADEFSSDPSDIAKDLRTSPFTLRLHFDNLGCKFIRKNNLS 420
           RLSAEKTDLLISHVLVL LF DEFSSDPSDIA+DLRTSPFTLR+H+DNLGCKFIRKNNLS
Sbjct: 361 RLSAEKTDLLISHVLVLALFPDEFSSDPSDIARDLRTSPFTLRVHYDNLGCKFIRKNNLS 420

Query: 421 LVTLPIPLQFPELRQKRRR 439
           LVTLP+PLQFPELR+KRRR
Sbjct: 421 LVTLPVPLQFPELRRKRRR 437

BLAST of Lcy05g001040 vs. NCBI nr
Match: XP_022151610.1 (DNA-directed RNA polymerase I subunit rpa49 [Momordica charantia])

HSP 1 Score: 766.5 bits (1978), Expect = 1.2e-217
Identity = 395/439 (89.98%), Postives = 416/439 (94.76%), Query Frame = 0

Query: 1   MAEDSRSFELEETHYTEPEANSQSPAKK-KKSEKKKKRVPPVTAKIQVLSNHPEKTPPLV 60
           MA D++SFELEETHY EPEANSQSPAKK +KSEKKKKRV  V A IQVLSNHPEKTPPLV
Sbjct: 1   MAADTKSFELEETHYAEPEANSQSPAKKNRKSEKKKKRVSSVNATIQVLSNHPEKTPPLV 60

Query: 61  GYFPSGFDPHKDADEEAVEEQNSVRARVYRHKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120
           GYFPSGF+PHKDADEEAV+EQ S+RARVYR+KNKPNRMQLVVSPTGSNVDFVGTNYLGEA
Sbjct: 61  GYFPSGFNPHKDADEEAVQEQKSIRARVYRNKNKPNRMQLVVSPTGSNVDFVGTNYLGEA 120

Query: 121 TAGKQHCTYALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVPEKESPCVANDELSVQE 180
           TAGKQHCTYALGVLDKATQSLKIVPIA+NMIFRLDPKVRGSDV EKESP V +DELSVQE
Sbjct: 121 TAGKQHCTYALGVLDKATQSLKIVPIAANMIFRLDPKVRGSDVSEKESPTVVDDELSVQE 180

Query: 181 KADRMRELTNLYGTKRSIKQAKKLHSLKQEDDPDTKKDLDVKMKNVVVNKEALESTNAQI 240
           KADRMRELTNLYGTKRSIKQAKKLHSLKQ DD DT+KDLD KMKNVVVNKEALE TNA+I
Sbjct: 181 KADRMRELTNLYGTKRSIKQAKKLHSLKQVDDSDTQKDLDEKMKNVVVNKEALEGTNAEI 240

Query: 241 ARNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFLLQEEAEISANYPSFVRNRIYKL 300
           ARNIPTYNASATTPQEAYPLDKIIL GEWS+LED+Y LL+ EAEISANYPSFVRNRIYKL
Sbjct: 241 ARNIPTYNASATTPQEAYPLDKIILMGEWSYLEDVYSLLRAEAEISANYPSFVRNRIYKL 300

Query: 301 QDVQDEEEKKKLCCIFSYITHLIKYKDQHSMDGVSSAKGHKIPSMLRQKFSSMFSTTESR 360
           QD+QDEEEKKKLCCIFSYITHLIK+KDQ+SMDGVSSAKGHKIPSMLRQKFSSMFS  ESR
Sbjct: 301 QDIQDEEEKKKLCCIFSYITHLIKFKDQNSMDGVSSAKGHKIPSMLRQKFSSMFSLLESR 360

Query: 361 RLSAEKTDLLISHVLVLTLFADEFSSDPSDIAKDLRTSPFTLRLHFDNLGCKFIRKNNLS 420
           RLS EK+DLLISHVLVLTLFADEFSSDPSDIAKDLRTSPFTLRLHFDNLGCKFIRKNNLS
Sbjct: 361 RLSPEKSDLLISHVLVLTLFADEFSSDPSDIAKDLRTSPFTLRLHFDNLGCKFIRKNNLS 420

Query: 421 LVTLPIPLQFPELRQKRRR 439
           LVTLP+PL+FP+L  KRRR
Sbjct: 421 LVTLPVPLKFPQLSLKRRR 439

BLAST of Lcy05g001040 vs. TAIR 10
Match: AT3G13940.1 (DNA binding;DNA-directed RNA polymerases )

HSP 1 Score: 352.8 bits (904), Expect = 3.9e-97
Identity = 192/441 (43.54%), Postives = 287/441 (65.08%), Query Frame = 0

Query: 3   EDSRSFELEETHYTEPEANSQSPAKKKK----SEKKKKRVPPVTAKIQVLSNHPEKTPPL 62
           E+   +  EE  +  P+   +   K KK    +E + +    VT K+  ++  P++  P+
Sbjct: 4   EEKNDYRNEEEDFNTPQQLQKKQRKGKKIATETEDENEEDKRVTLKVTQVAERPDRISPI 63

Query: 63  VGYFPSGFDPHKDADEEAVEEQNSVRARVYRHK-NKPNRMQLVVSPTGSNVDFVGTNYLG 122
           V YF +G+DP K   +       + +  VY+HK +   R+Q+VVSP G+ V+FVGTNY G
Sbjct: 64  VAYFSTGYDPCKVDPKTGKRVHETPKVTVYKHKDDSKKRIQVVVSPPGARVEFVGTNYTG 123

Query: 123 EATAGKQHCTYALGVLDKATQSLKIVPIASNMIFRLDPKVRGSDVPEKESPCVANDELSV 182
           E  A  Q  TY +GV ++  ++L+I+P+A N I RL+P+V+  +  E+E    A+    V
Sbjct: 124 E-QAAMQTNTYRVGVFNREAKTLRILPVAHNKIIRLEPRVKAQETNEEE----ASGSAVV 183

Query: 183 QEKADRMRELTNLYGTKRSIKQAKKLHSLKQEDDPDTKKDLDVKMKNVVVNKEALESTNA 242
           +E  +      + Y TK+++ + KK  +L   DD  T+K LD K+  + VN  ALE T++
Sbjct: 184 KELEELKTGERDRYNTKKAVTRDKKKRALYMGDDAATQKVLDGKLSELGVNTAALEGTSS 243

Query: 243 QIARNIPTYNASATTPQEAYPLDKIILKGEWSFLEDLYFLLQEEAEISAN-YPSFVRNRI 302
            +ARNIP YN +ATT  EAYPL+KII KG+WSFLED+Y+LLQ+E E +   YP FVRNR+
Sbjct: 244 TVARNIPPYNTAATTANEAYPLEKIIEKGDWSFLEDIYWLLQQETEAATEAYPVFVRNRL 303

Query: 303 YKLQDVQDEEEKKKLCCIFSYITHLIKYKDQHSMDGVSSAKGHKIPSMLRQKFSSMFSTT 362
           Y+L+D++D+ +K+ +C   + +THL+K+KD++SM+G  SAK HK+P + RQKF+SMF  +
Sbjct: 304 YRLRDIKDDMKKQTVCGAATLLTHLVKFKDRNSMNGYDSAKDHKMPDIFRQKFNSMFKDS 363

Query: 363 ESRRLSAEKTDLLISHVLVLTLFADEFSSDPSDIAKDLRTSPFTLRLHFDNLGCKFIRKN 422
           ES R+  +K +LLIS+VLVL+L  D F +DP DIAKDLR S   LR HF  LGCKF+++N
Sbjct: 364 ESDRIPVDKINLLISYVLVLSLHVDNFMTDPEDIAKDLRISTVELRKHFLQLGCKFLKQN 423

Query: 423 NLSLVTLPIPLQFPELRQKRR 438
           + ++ TLP PL FPE+ ++RR
Sbjct: 424 STTVATLPTPLNFPEVNRRRR 439

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O140863.9e-0924.71DNA-directed RNA polymerase I subunit rpa49 OS=Schizosaccharomyces pombe (strain... [more]
Match NameE-valueIdentityDescription
A0A6J1KIP64.3e-22190.64DNA-directed RNA polymerase I subunit rpa49-like OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1HM517.4e-22190.89DNA-directed RNA polymerase I subunit rpa49-like OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1DBN95.9e-21889.98DNA-directed RNA polymerase I subunit rpa49 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1JX252.0e-21387.93DNA-directed RNA polymerase I subunit rpa49-like OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1GQS48.2e-21287.24DNA-directed RNA polymerase I subunit rpa49-like OS=Cucurbita moschata OX=3662 G... [more]
Match NameE-valueIdentityDescription
KAG7025877.11.1e-22190.87DNA-directed RNA polymerase I subunit rpa49 [Cucurbita argyrosperma subsp. argyr... [more]
XP_023514670.15.3e-22191.12DNA-directed RNA polymerase I subunit rpa49-like [Cucurbita pepo subsp. pepo][more]
XP_023000003.19.0e-22190.64DNA-directed RNA polymerase I subunit rpa49-like [Cucurbita maxima] >XP_02300000... [more]
XP_022964095.11.5e-22090.89DNA-directed RNA polymerase I subunit rpa49-like [Cucurbita moschata] >XP_022964... [more]
XP_022151610.11.2e-21789.98DNA-directed RNA polymerase I subunit rpa49 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT3G13940.13.9e-9743.54DNA binding;DNA-directed RNA polymerases [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009668RNA polymerase I associated factor, A49-likePFAMPF06870RNA_pol_I_A49coord: 82..437
e-value: 9.5E-59
score: 199.2
IPR009668RNA polymerase I associated factor, A49-likePANTHERPTHR14440DNA-DIRECTED RNA POLYMERASE I SUBUNIT RPA49coord: 27..437
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..16
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..56

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy05g001040.1Lcy05g001040.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042790 nucleolar large rRNA transcription by RNA polymerase I
biological_process GO:0001188 RNA polymerase I preinitiation complex assembly
biological_process GO:0006362 transcription elongation from RNA polymerase I promoter
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005736 RNA polymerase I complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity