Lcy05g000880 (gene) Sponge gourd (P93075) v1

Overview
NameLcy05g000880
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationChr05: 886759 .. 891778 (+)
RNA-Seq ExpressionLcy05g000880
SyntenyLcy05g000880
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCTCATCGGAGCTAGCCCTCGTTTCTCTGGTCTCCCGGTTTGCTTTATCGTTTGATGGAGCCGTTTTGGGCGCAGCCCTAGCTTACGCTACCGTTCGTACCGTCCTCAAACTCACTGCTTCCTGTTCCGCTCTCAAAGAGCTCCGTAAAGCTCCTTATGTTAGAGTTTCTGATCTTCGCCATATCATTTCTGAAGGAGATTCGGAACAATCCGGGTCTGATCAGAAGCTTGTTGTAGTTCGTGGCACCGTGGAGGCCAGATCGTCCGTGGAGGGTAATTGGAAGAGCTTACAACCTAATGTATTGATTTCTCAAGAGTCCGGAGATAGAGCGGTGATTATTCAGCGAACTCAAATGGTAACTCCTCTGGTATTTCTGTTAGTATCTGCGTCATTGTGATGATACATATATATATTCTCTTTTTGGGGCTGCCGTTCTTCTGTAGGGGGATGGATGAAAACTGAGCTATGCAGTTTAGATTGCGTGATTAAAATTAATATTATTCCGTTTGTTAGAATGTTATGATTGCATAGTGTCGTGACAGTATTCGAGTAATTTGATAGCACCACTAAGATTGCTTATAAAGTTTTAACCAATTAAATTGTTTTCACATTACAAGTTCTCTAGTTTAGGTCCTCGGTTCAAGAGGCAGTTAAGATATGATTCTAGTCTGGATGAACAATGAAAGAGAAGGATCACCGTTTGAAATGACTGTTCAATTTATTTGCATAGTTCGGCTGGCTTGCCTGTGTTATATCTGTCACTCATTAATTTGTATAAGTATGATGTGCGGATTAAATATTGGGAAATCTTGAATTCCTTAATCAACATAATTAACTTATGAATTTAATCTTGGGGAAATGCATATCTCTTTTGCCAGTGTTACCTATCTTAACCTCATTGGTTCTGCAATTGTCTGTTTTTGTTCCCCTTGTTATTCAGTGTTATGGATGTGTGTATATAATATCCAGTATTATAAATTGAAGCCTTTTTGGTTGTAAATTCTGGCATTGTGTAGTGCATATTCAACGAATGGAAAGGCTTTTTTGGATGGACTTCTGATATACGTGCAATTATTGGAAGAACCTTTAGAAAGCAAGAGTCAACATCATTTAGAACGGTAAAGTCAAGGCTTCTTTCTATTATTTCTATGATTGATTCACTCTTTTCGTTACCCTTACTGACAAAAGATCTTTGTATTATATATAGCCATTTACATTTCCACTTGAATGATGGACCTTTGTTACTCCTTCTATGAATAAAGAACCAATTGAAGTTGAATGATTTTTTATGTGGGGCATTTCTTGAATATGGGGCCATTCTGTTAGGTCCTTTTCCTGTGATTCTCTATTGGGTCTTTTTTTTTCTCTCGCTCATGGTGCGTGCAATTAGGTTTGTAAGTGCCACCTCTTTAGAAACGAGTTTTAACAGAGTAGGCTTGAAGCCCTCGCTATGGCTGAGATGATGGGGTGTAGGATTGAGAAAATTCCATTTAACTACGTGGGGGTCTCTGTTGGCGGTAGGCCTAAGAAGGAAGCGTTCTGGAACCGGTAATAGACAACATTCGCAGCAAGTTAGCTCTTTGGAAAGGTTTCCCCATCTCCAAAGGTGGTAGAACTACCGTCATAAAGGCTGTTTTATCTAGCATCCCAGCCTATTTTCTTTCTATTTTCAGGATACCAGTGGGGGTGAGCAAATGCATTGAGAAGTATTTTAGAAGATTCCTTTGGAAAGGGGCTGATGATATTAAAGGTTCCCATTTGGTGAATTGGGAGGCTGTGACAACTCTTAAATCGTTAGGAGGGCTTGGTATTGATAACATAAGAGCTAAGAACATGGCTCTTCTCTCAAAGTGGCTTTGGAGATTTGAAAAGGAGCCCGAGGCCTTATGGAGGAAGATCATTCTCAGTAAGTATGGGGATGGCTTTACTCGAGACCCCTTGAGCTATAAAAAGTGGAAATCGGTTTCCAGTTCGTGGAAGGATATAGCTAGATTAGCTGGTTTCTTCAAAAATAATAGTAAGTTAAGTATAAGGTGGGTAAAGGGGACAAAGCTTTGTTTTGGGAGGATGCCTGAGTGTCTGAAAGGCTCTTAAAGGATATGTTCCCTCGTATGTTTGTCATTTCGATGGCTAAAAAGGTGAAAGTTAACGAGCTTTGGAATGACTACTCCTGGGATCTTAGACTTAGAAGAGGAATGCTCGATAGGGAGGCTAACAAATGGTTTGGGCTTTGCTCTACTTTATCTCAGCACAGTTTCAACGGTAATGATGATGTCATTTGGTGGAAGCCTGAAAACTCTGGCATTTTCTCTGTTAAATCTGCTCTCTTAGCTTTGCAGCTGAATAGAAGATTGTTGGTGGAGGAGTTGAGCTCTCAAATATGGGAAGGTACCATTCCTAATAGGGTGAAGTTGTTCTTTTGGACTTTAGCTCTTAATGGAATCAACACTATGGACATGGTGCAGAACATGTGTGTTATGTGCTGTAGAAATGAGGAATCGGCTTCTCACCTATTGCTTCATTGTGATTTTGCGGTTCAAGTGTGGAATCGGTTTGCTGAAGGCTTTGGGCAGAAAGGTTGCAAGCCCAGAAATGTGGTGGATTGGTTACCTGAGATTTTGGTTGGAGGAGGATTAAAAGACAAAGCAAGAATCCTGTGGAAAAATTTCTCTAGAGCCATCATTTGGCACCTATGGAAGGAAAGAAATGCAAGGGTCTTCGCAGATAAGTTCACGCCTATAGATATTTTTTGTGATATTGTACAGCTTTCGGCCTCTAATTGGAGTGCCCAAGATAGATTATTTTGTAATTACTCAGCCGCCATGATTAACCTTCAATGGAAGGCTTTTTTGTAGTTTCTTTGGAGGTAGGGATGCCTTATCCCACCGTCCTTAGGCTGCTTTGGCGTTGGCTTTTGTGAATGAAGATGTCTATTTCTTCTCTTAAAAAAAAAGAAAAAAACAGAGTAGGCTTGAAGACATACCCAATAGTGTCAAGAAATAAGGAATTTATGGTAGCTAACTTGTTGTCTTTAAAAATTAAATCTACTTAATATGAGATTGATGTGTAGCCTTTGTGCTTCCTCCAGCCCCGCTATGGTTGTGATTTGTGAAGGTAAATTATATATTTTAGATATCTGGTTGAGCAATAGGGAACTATATTCCACTTATGATTGTACCAGAGTTCAGTGCAGTTTGCATATAAACTCTTATGTAATGCTGATATTTGAGAAATTTTTAACAGGTTCCTTTTGTCCTCGTTGAGGGTCCTTCACCATATTCTGATTTTATTGTTGTTAACATGGATGGCTCAAGACATCCCTTGCCTCTTACAACTGTGTATCATCAACTGGAGCCCGTTTGTGCTACTCCTTATACCTTCCTTCAAGCACTTTTTGGTCATGAGTACCCTGTGAGTTAGGCAATCTATATTGAAGTTGAATAACTTTTCTTTTATGGATTTTAGCTAAAATCTCTTTATCTTATTCTACTTTTGAAGGTTGGGGTGCTTGATGAGGAGAAAATACTTCCATTAGGAAAAAATATCAGTGCTGTTGGCATATGCAGTTTTAGAAATGGAGTTCCCGTGATAAAGTCATGCAAGGATTTTCCCCATTTCCTGTAAGATTATACATTTTGAAGTTGAAATAAAGTTGAGAAGGTCAAAATTATGTTATGTAATTTATGAAAGGTAGATCATATATGCATATCGTTTATTCTTTCCCTTGACTCATCCACATAAAATTGATAATAAATGTGTTGTTGTCAAACAGTGAGCCTCATTGATTTGGGTTTGGGGCTCTCGATAGGATTTTAACTAGGTCCTAGGATCAAGCATCTGAATAGAAAGCTTAAAAAGAAAACTTTGTCTGTTGGAGCTATATGGATTTGTATGGATTTGTTGTATAGGTCCCAATACTCTCTCTCTACACACACACACATGTGTGTGTTCCCATTTCCAGTTGAAGTGTGGGTTTTGGCTTATACCTAAGTTAATGATGCTACCAAATGGCTAGTCTCTCCCACACCCACCCAAAGAAAAAAAAAAAGAATAAATTGATTTTGCCTTTTGTTTGTAATAAACTGACTTCGAGATAATCTACATTGGAATAACGGATGTTATGGTGATATACTTTTCTTTGCAGATGTGAAATGACCAAGGATCAGATGATTTTAGATCTAGTCTTCAAAACAAAGATTCTGTTCTGGAGTAGTATTGTCCTTGGTTCGTTGTCAGTTGGCATTCTTGGCTATGCTGTTGTGAGGTATGCTTCCATTCTTTCTTAGTTTCTTTCTTCTTTTATTTTATTTTCTTGTCAAACTGTCGTGAGGTATCTTAATGTTGATATTTTTTTAGGCGAAGCAATGCACTCGTAATATATACCTTCCATGAAATGTTTATTTTGGATTCAATGCTGAAATGATCTTCAATCTTTTCTTTTTTGCCGTTGAATTTCTTTTACTTTTATGAACTCACCAATGTTCTTAATTGGACCTCAAATGCTGGTATGAATGATAACTTAGCTCAACTGTGTAGAGCGTGAGTGATGAATGACGAGACTGTTTCCAAGTATACTCACTTGTTTGGTTGCTTTCCTTCATCAAAAAATGATTACATTGTAAAACTTTGATGAATGTTTGAACTTAGTAGTGTCCATTCTCTTCAGAAATTGGAATAAATGGAAACGGTGGACGCAACAGGGACGGTTACAGGATTCAGGGAATGATTCCACCATGTCCCAACTGGATTCAAGCAATAATATTCCCATTTATGGTGATTCTGGAACTGCCTTAGACGACGAGGTAGCAGCGGTTTCAGATGACGAGGTATTGAACATTCCAGATGGACAGTTGTGCGTGATCTGTCTCATGAGGAGAAAGCGATCTGCTTTCATTCCATGTGGTCATTTAGTTTGCTGCGAACGCTGTGCCATATCGGTCGAACGTGATTCAGCTCCCAAATGTCCTGTCTGTCGCCAGCAGATCCATCGTTCTGTGCGGATATATGACTCATGA

mRNA sequence

ATGTCCTCATCGGAGCTAGCCCTCGTTTCTCTGGTCTCCCGGTTTGCTTTATCGTTTGATGGAGCCGTTTTGGGCGCAGCCCTAGCTTACGCTACCGTTCGTACCGTCCTCAAACTCACTGCTTCCTGTTCCGCTCTCAAAGAGCTCCGTAAAGCTCCTTATGTTAGAGTTTCTGATCTTCGCCATATCATTTCTGAAGGAGATTCGGAACAATCCGGGTCTGATCAGAAGCTTGTTGTAGTTCGTGGCACCGTGGAGGCCAGATCGTCCGTGGAGGGTAATTGGAAGAGCTTACAACCTAATGTATTGATTTCTCAAGAGTCCGGAGATAGAGCGGTGATTATTCAGCGAACTCAAATGTGCATATTCAACGAATGGAAAGGCTTTTTTGGATGGACTTCTGATATACGTGCAATTATTGGAAGAACCTTTAGAAAGCAAGAGTCAACATCATTTAGAACGGTTCCTTTTGTCCTCGTTGAGGGTCCTTCACCATATTCTGATTTTATTGTTGTTAACATGGATGGCTCAAGACATCCCTTGCCTCTTACAACTGTGTATCATCAACTGGAGCCCGTTTGTGCTACTCCTTATACCTTCCTTCAAGCACTTTTTGGTCATGAGTACCCTGTTGGGGTGCTTGATGAGGAGAAAATACTTCCATTAGGAAAAAATATCAGTGCTGTTGGCATATGCAGTTTTAGAAATGGAGTTCCCGTGATAAAGTCATGCAAGGATTTTCCCCATTTCCTATGTGAAATGACCAAGGATCAGATGATTTTAGATCTAGTCTTCAAAACAAAGATTCTGTTCTGGAGTAGTATTGTCCTTGGTTCGTTGTCAGTTGGCATTCTTGGCTATGCTGTTGTGAGAAATTGGAATAAATGGAAACGGTGGACGCAACAGGGACGGTTACAGGATTCAGGGAATGATTCCACCATGTCCCAACTGGATTCAAGCAATAATATTCCCATTTATGGTGATTCTGGAACTGCCTTAGACGACGAGGTAGCAGCGGTTTCAGATGACGAGGTATTGAACATTCCAGATGGACAGTTGTGCGTGATCTGTCTCATGAGGAGAAAGCGATCTGCTTTCATTCCATGTGGTCATTTAGTTTGCTGCGAACGCTGTGCCATATCGGTCGAACGTGATTCAGCTCCCAAATGTCCTGTCTGTCGCCAGCAGATCCATCGTTCTGTGCGGATATATGACTCATGA

Coding sequence (CDS)

ATGTCCTCATCGGAGCTAGCCCTCGTTTCTCTGGTCTCCCGGTTTGCTTTATCGTTTGATGGAGCCGTTTTGGGCGCAGCCCTAGCTTACGCTACCGTTCGTACCGTCCTCAAACTCACTGCTTCCTGTTCCGCTCTCAAAGAGCTCCGTAAAGCTCCTTATGTTAGAGTTTCTGATCTTCGCCATATCATTTCTGAAGGAGATTCGGAACAATCCGGGTCTGATCAGAAGCTTGTTGTAGTTCGTGGCACCGTGGAGGCCAGATCGTCCGTGGAGGGTAATTGGAAGAGCTTACAACCTAATGTATTGATTTCTCAAGAGTCCGGAGATAGAGCGGTGATTATTCAGCGAACTCAAATGTGCATATTCAACGAATGGAAAGGCTTTTTTGGATGGACTTCTGATATACGTGCAATTATTGGAAGAACCTTTAGAAAGCAAGAGTCAACATCATTTAGAACGGTTCCTTTTGTCCTCGTTGAGGGTCCTTCACCATATTCTGATTTTATTGTTGTTAACATGGATGGCTCAAGACATCCCTTGCCTCTTACAACTGTGTATCATCAACTGGAGCCCGTTTGTGCTACTCCTTATACCTTCCTTCAAGCACTTTTTGGTCATGAGTACCCTGTTGGGGTGCTTGATGAGGAGAAAATACTTCCATTAGGAAAAAATATCAGTGCTGTTGGCATATGCAGTTTTAGAAATGGAGTTCCCGTGATAAAGTCATGCAAGGATTTTCCCCATTTCCTATGTGAAATGACCAAGGATCAGATGATTTTAGATCTAGTCTTCAAAACAAAGATTCTGTTCTGGAGTAGTATTGTCCTTGGTTCGTTGTCAGTTGGCATTCTTGGCTATGCTGTTGTGAGAAATTGGAATAAATGGAAACGGTGGACGCAACAGGGACGGTTACAGGATTCAGGGAATGATTCCACCATGTCCCAACTGGATTCAAGCAATAATATTCCCATTTATGGTGATTCTGGAACTGCCTTAGACGACGAGGTAGCAGCGGTTTCAGATGACGAGGTATTGAACATTCCAGATGGACAGTTGTGCGTGATCTGTCTCATGAGGAGAAAGCGATCTGCTTTCATTCCATGTGGTCATTTAGTTTGCTGCGAACGCTGTGCCATATCGGTCGAACGTGATTCAGCTCCCAAATGTCCTGTCTGTCGCCAGCAGATCCATCGTTCTGTGCGGATATATGACTCATGA

Protein sequence

MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDLRHIISEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQMCIFNEWKGFFGWTSDIRAIIGRTFRKQESTSFRTVPFVLVEGPSPYSDFIVVNMDGSRHPLPLTTVYHQLEPVCATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVPVIKSCKDFPHFLCEMTKDQMILDLVFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRWTQQGRLQDSGNDSTMSQLDSSNNIPIYGDSGTALDDEVAAVSDDEVLNIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIHRSVRIYDS
Homology
BLAST of Lcy05g000880 vs. ExPASy Swiss-Prot
Match: Q9SYH3 (E3 ubiquitin-protein ligase SPL2 OS=Arabidopsis thaliana OX=3702 GN=SPL2 PE=2 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.5e-100
Identity = 204/413 (49.39%), Postives = 274/413 (66.34%), Query Frame = 0

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSS E AL++L++  ALSFDGA+LG  LA + V + LK  ++ +ALK+++ AP V +SDL
Sbjct: 1   MSSPERALLNLLTDIALSFDGAILGLTLAVSAVGSALKYASTNAALKKIKDAPEVSISDL 60

Query: 61  RHII------SEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVI 120
           R ++      SE +  +  +DQ++VVVRG V+ + S +  +K+   NVLIS E+GD+A+I
Sbjct: 61  RSLLPASEDKSETNDNRKSNDQRIVVVRGVVKPKISGDEGYKN--NNVLISPETGDKALI 120

Query: 121 IQRTQMCIFNEWKGFFGWTSDIRAIIGRTFRKQESTSFRTVPFVLV-EGPSPYSDFIVVN 180
           IQRTQ  +++ WK  F  T   R ++ R+ RK  +   RTVPFV+V +     S F+ VN
Sbjct: 121 IQRTQTYVYSGWKRLFQSTGH-RFMLERSLRKHGADFTRTVPFVIVGKDQQSNSSFVAVN 180

Query: 181 MDGSRHPLPLTTVYHQLEPVCATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICS 240
           MDGSR PLPLTTVY++L+P+ +   +FLQA    +YPVG+LD EKILP GK+I+AVGI S
Sbjct: 181 MDGSRQPLPLTTVYNRLQPINS---SFLQAFLYPDYPVGLLDIEKILPPGKDITAVGIYS 240

Query: 241 FRNGVPVIKSCKDFPHFLCEMTKDQMILDLVFKTKILFWSSIVLGSLSVGILGYAVVRNW 300
           F NGVP IKSC+D P+FL EMTKD+MI DL+ +T  +F  S++LG +SVGIL YA VR W
Sbjct: 241 FNNGVPEIKSCQDLPYFLSEMTKDKMIEDLMEQTNFIFLGSVILGIVSVGILSYAAVRTW 300

Query: 301 NKWKRWTQQGRLQDSGNDSTMSQLDSSNNIPIYGDSGTALDDEVAAVSDDEVLNIPDGQL 360
           NKWK+W  Q  L    NDS                    +DDE      ++   IPDG+L
Sbjct: 301 NKWKQWNHQRELPQRPNDS-------------------VVDDE-----PEDADEIPDGEL 360

Query: 361 CVICLMRRKRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIHRSVRIYDS 407
           CVIC+ RR+  AFIPCGH+VCC RCA +VER+  PKCPVC Q I  S+R+Y S
Sbjct: 361 CVICVSRRRVPAFIPCGHVVCCRRCASTVERELNPKCPVCLQSIRGSMRVYYS 383

BLAST of Lcy05g000880 vs. ExPASy Swiss-Prot
Match: Q94HV7 (E3 ubiquitin-protein ligase SPL1 OS=Arabidopsis thaliana OX=3702 GN=SPL1 PE=2 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 3.6e-09
Identity = 66/262 (25.19%), Postives = 123/262 (46.95%), Query Frame = 0

Query: 153 RTVPFVLVEGPSPYSDFIVVNMDGSRHPLPL-----TTVYHQLEPVCATPYTFLQALFGH 212
           + VP+ L +G         VN+D S+  L L     + V+ + EPV     + +Q   G+
Sbjct: 107 KEVPWYLDDGTGR------VNVDVSQGELGLALTVGSDVFEKAEPV-----SLVQGALGY 166

Query: 213 EYP---VGVLDEEKILPLGKNISAVGICSFRNGVPVIKSCK--DFPHFLCEMTKDQMILD 272
                 +GV   E+++P+G  ++ VG  + R+G+  ++  K    P ++  +  DQ+I  
Sbjct: 167 LKGFKILGVRHVERVVPIGTPLTVVGE-AVRDGMGNVRIQKPEQGPFYVTYIPLDQLISK 226

Query: 273 LVFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRWTQQGRLQDSGNDSTMSQLDSSNN 332
           L   ++   ++S+ L  L V ++   V+    K    T + R +       +    +   
Sbjct: 227 LGDLSRRFKYASMGLTVLGVILISKPVIEYILKRIEDTLERRRRQFALKRVVDAA-ARRA 286

Query: 333 IPIYGDSGTALDDEVAAVSDDEVLNIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISV 392
            P+ G  GT+ D +            PD  LCV+CL ++  +AF+ CGH+ CC  C++ +
Sbjct: 287 KPVTG-GGTSRDGDT-----------PD--LCVVCLDQKYNTAFVECGHMCCCTPCSLQL 336

Query: 393 ERDSAPKCPVCRQQIHRSVRIY 405
                  CP+CR++I + ++IY
Sbjct: 347 R-----TCPLCRERIQQVLKIY 336

BLAST of Lcy05g000880 vs. ExPASy Swiss-Prot
Match: P41437 (E3 ubiquitin-protein ligase IAP-3 OS=Orgyia pseudotsugata multicapsid polyhedrosis virus OX=262177 GN=IAP3 PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.5e-07
Identity = 26/57 (45.61%), Postives = 35/57 (61.40%), Query Frame = 0

Query: 348 IPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIHRSVRIY 405
           + D +LC ICL   K   F+PCGH+V C +CA  V       CPVCR Q+ ++VR+Y
Sbjct: 215 VADDRLCKICLGAEKTVCFVPCGHVVACGKCAAGV-----TTCPVCRGQLDKAVRMY 266

BLAST of Lcy05g000880 vs. ExPASy Swiss-Prot
Match: O08863 (Baculoviral IAP repeat-containing protein 3 OS=Mus musculus OX=10090 GN=Birc3 PE=1 SV=2)

HSP 1 Score: 56.6 bits (135), Expect = 7.5e-07
Identity = 26/71 (36.62%), Postives = 41/71 (57.75%), Query Frame = 0

Query: 334 DDEVAAVSDDEVLNIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISVERDSAPKCPVC 393
           DD  A   ++++  + + ++C +C+ R     FIPCGHLV C+ CA S+      KCP+C
Sbjct: 533 DDIAALPMEEQLRKLQEERMCKVCMDREVSIVFIPCGHLVVCKDCAPSLR-----KCPIC 592

Query: 394 RQQIHRSVRIY 405
           R  I  +VR +
Sbjct: 593 RGTIKGTVRTF 598

BLAST of Lcy05g000880 vs. ExPASy Swiss-Prot
Match: Q24306 (Death-associated inhibitor of apoptosis 1 OS=Drosophila melanogaster OX=7227 GN=Diap1 PE=1 SV=2)

HSP 1 Score: 56.6 bits (135), Expect = 7.5e-07
Identity = 26/60 (43.33%), Postives = 37/60 (61.67%), Query Frame = 0

Query: 347 NIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIHRSVRIYDS 406
           +IP+ +LC IC      +AF+PCGH+V C +CA SV      KCP+CR+     +R+Y S
Sbjct: 384 SIPEEKLCKICYGAEYNTAFLPCGHVVACAKCASSV-----TKCPLCRKPFTDVMRVYFS 438

BLAST of Lcy05g000880 vs. ExPASy TrEMBL
Match: A0A6J1HK84 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111464304 PE=4 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 1.1e-205
Identity = 360/407 (88.45%), Postives = 378/407 (92.87%), Query Frame = 0

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSSSE ALVSL SRFALSFDGA LGAALAYA  RTVLKLTASCSAL ELRKAP VRVSDL
Sbjct: 1   MSSSEQALVSLFSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60

Query: 61  RHIISEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RH+IS+GDS QS SDQKLVVVRGTVEARS VEGNWKSLQPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHMISDGDSRQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFFGWTSDIRAIIGRTFRKQESTSFRTVPFVLVEGPSPYSDFIVVNMDGSRHP 180
           CIFNEWKGF GWTSD+RAI GRTFRKQESTSFRTVPFVLVEG SPYSDF VVNMDGSRH 
Sbjct: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFAVVNMDGSRHR 180

Query: 181 LPLTTVYHQLEPVCATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVPV 240
           LPLTTVY QL+PVCATPYTFLQALFGHEYPVGVLDEEKILPLG+NIS VG+CSF+NGVP 
Sbjct: 181 LPLTTVYRQLQPVCATPYTFLQALFGHEYPVGVLDEEKILPLGRNISVVGVCSFKNGVPE 240

Query: 241 IKSCKDFPHFLCEMTKDQMILDLVFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRWT 300
           IKSCKDFPHFLCEMTKDQM+LDLVFKTK LFWSSIVLGSLSVG+LG +V+RNWN+WK+W 
Sbjct: 241 IKSCKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR 300

Query: 301 QQGRLQDSGNDSTMSQLDSSNNIPIYGDSGTALDDEVAAVSDDEV-LNIPDGQLCVICLM 360
           QQ RLQDSGNDSTMSQLDSSNNIPIYGD GTA DDEVA++ DDE+  N+PDGQLCVICLM
Sbjct: 301 QQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM 360

Query: 361 RRKRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIHRSVRIYDS 407
           RR+RSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQI  SVRIYDS
Sbjct: 361 RRRRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 407

BLAST of Lcy05g000880 vs. ExPASy TrEMBL
Match: A0A6J1JT39 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488642 PE=4 SV=1)

HSP 1 Score: 724.9 bits (1870), Expect = 1.8e-205
Identity = 364/408 (89.22%), Postives = 382/408 (93.63%), Query Frame = 0

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSSSE ALVSL+S+FALSFDGAVLGAALAYA VRTVLKLTASCSAL ELRKAPYV VSDL
Sbjct: 1   MSSSEQALVSLLSQFALSFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIISEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RHIIS+GDS +S SD KLVVVRGTVEARSSVEGNWKS+QPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHIISDGDSGKSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFFGWTSDIRAIIGRTFRKQESTSFRTVPFVLV-EGPSPYSDFIVVNMDGSRH 180
           CIFNEWKGFFGWTSD+RAI GRTFRKQESTSFRTVPFVLV EG SPYSDF+VVNMDGSRH
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDEGHSPYSDFVVVNMDGSRH 180

Query: 181 PLPLTTVYHQLEPVCATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVP 240
           PLPLTTVYHQL+PV ATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSF+NGVP
Sbjct: 181 PLPLTTVYHQLQPVSATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFKNGVP 240

Query: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRW 300
           VIKSCKDFPHFLCEMTKDQMILDLVFKTK LFWSS+VLGSLSVG+LGY+VVRNWN+WK+W
Sbjct: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNRWKQW 300

Query: 301 TQQGRLQDSGNDSTMSQLDSSNNIPIYGDSGTALDDEVAAVSDDEV-LNIPDGQLCVICL 360
            QQ RLQDSGNDSTMSQLD SNNIPIYGD GTA D+E A   +DEV  N+PDGQLCVICL
Sbjct: 301 RQQRRLQDSGNDSTMSQLD-SNNIPIYGDFGTASDNEEAVDPEDEVSSNVPDGQLCVICL 360

Query: 361 MRRKRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIHRSVRIYDS 407
           MRRKRSAFIPCGH VCCERCA+SVERDSAPKCPVCRQQI  SVRIYDS
Sbjct: 361 MRRKRSAFIPCGHFVCCERCAMSVERDSAPKCPVCRQQIRSSVRIYDS 407

BLAST of Lcy05g000880 vs. ExPASy TrEMBL
Match: A0A6J1GS48 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111456504 PE=4 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 5.3e-205
Identity = 364/408 (89.22%), Postives = 381/408 (93.38%), Query Frame = 0

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSSSE ALVSL+S+FALSFDGAVLGAALAYA VRTVLKLTASCSAL ELRKAPYV VSDL
Sbjct: 1   MSSSEQALVSLLSQFALSFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIISEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RHIIS+GDS QS SD KLVVVRGTVEARSSVEGNWKS+QPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHIISDGDSGQSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFFGWTSDIRAIIGRTFRKQESTSFRTVPFVLV-EGPSPYSDFIVVNMDGSRH 180
           CIFNEWKGFFGWTSD+RAI GRTFRKQESTS RTVPFVLV EG SPYSDF+VVNMDGSRH
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSLRTVPFVLVDEGHSPYSDFVVVNMDGSRH 180

Query: 181 PLPLTTVYHQLEPVCATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVP 240
           PLPLTTVYHQL+PV ATPYTFLQALFGHEYPVGVLDEEKILPLGK ISAVGICSF+NGVP
Sbjct: 181 PLPLTTVYHQLQPVSATPYTFLQALFGHEYPVGVLDEEKILPLGKKISAVGICSFKNGVP 240

Query: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRW 300
           VIKSCKDFPHFLCEMTKDQMILDLVFKTK LFWSS+VLGSLSVG+LGY+VVRNWN+WK+W
Sbjct: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNRWKQW 300

Query: 301 TQQGRLQDSGNDSTMSQLDSSNNIPIYGDSGTALDDEVAAVSDDEV-LNIPDGQLCVICL 360
            QQ RLQDSGNDSTMSQLD SNNIPIYGD GTA D+E AA  +DEV  N+PDGQLCVICL
Sbjct: 301 RQQRRLQDSGNDSTMSQLD-SNNIPIYGDFGTASDNEEAADPEDEVSSNVPDGQLCVICL 360

Query: 361 MRRKRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIHRSVRIYDS 407
           MRRKRSAFIPCGH VCCERCA+SVERDSAPKCPVCRQQI  SVRIYDS
Sbjct: 361 MRRKRSAFIPCGHFVCCERCAMSVERDSAPKCPVCRQQIRSSVRIYDS 407

BLAST of Lcy05g000880 vs. ExPASy TrEMBL
Match: A0A6J1KHU7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111494539 PE=4 SV=1)

HSP 1 Score: 722.6 bits (1864), Expect = 9.0e-205
Identity = 358/407 (87.96%), Postives = 378/407 (92.87%), Query Frame = 0

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSSSE ALVSL SRFALSFDGA LGAALAYA  RTVLKLTASCSAL ELRKAP VRVSDL
Sbjct: 1   MSSSEQALVSLFSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60

Query: 61  RHIISEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RH+ S+GDS QS SDQKLVVVRGTVEARS VEGNWKSLQPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHMTSDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFFGWTSDIRAIIGRTFRKQESTSFRTVPFVLVEGPSPYSDFIVVNMDGSRHP 180
           CIFNEWKGF GWTSD+RAI GRTFRKQESTSFRTVPFVLVEG SPYSDF+VVNMDGSRH 
Sbjct: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHR 180

Query: 181 LPLTTVYHQLEPVCATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVPV 240
           LPLTTVY QL+PVCATPYTFLQALFGH+YPVGVLDEEKILPLGKNIS VG+CSF+NGVP 
Sbjct: 181 LPLTTVYRQLQPVCATPYTFLQALFGHKYPVGVLDEEKILPLGKNISVVGVCSFKNGVPE 240

Query: 241 IKSCKDFPHFLCEMTKDQMILDLVFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRWT 300
           IKSCKDFPHFLCEMTKDQM+LDLVFKTK LFWSSIVLGSLSVG+LG +V+RNWN+WK+W 
Sbjct: 241 IKSCKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR 300

Query: 301 QQGRLQDSGNDSTMSQLDSSNNIPIYGDSGTALDDEVAAVSDDEV-LNIPDGQLCVICLM 360
           QQ RLQDSGNDST+SQLDSSNNIPIYGD GTA DDEVA++ DDE+  N+PDGQLCVICLM
Sbjct: 301 QQRRLQDSGNDSTISQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM 360

Query: 361 RRKRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIHRSVRIYDS 407
           RR+RSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQI  SVRIYDS
Sbjct: 361 RRRRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 407

BLAST of Lcy05g000880 vs. ExPASy TrEMBL
Match: A0A5D3BWQ0 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001240 PE=4 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 8.4e-203
Identity = 354/407 (86.98%), Postives = 379/407 (93.12%), Query Frame = 0

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MS+SE ALV+L+SRFALSFDGAVLGAALAYATVRTVLKLTASCSAL ELRKAPYV VSDL
Sbjct: 1   MSTSEQALVTLLSRFALSFDGAVLGAALAYATVRTVLKLTASCSALNELRKAPYVTVSDL 60

Query: 61  RHIISEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RHI+S+G+SEQS SD+KLVVVRGTVE RSSVEGNWKSLQPNVLISQESGDRAVIIQRTQ+
Sbjct: 61  RHIVSDGNSEQSESDRKLVVVRGTVETRSSVEGNWKSLQPNVLISQESGDRAVIIQRTQV 120

Query: 121 CIFNEWKGFFGWTSDIRAIIGRTFRKQESTSFRTVPFVLVEGPSPYSDFIVVNMDGSRHP 180
           CIFNEWKGFFGWTSD+RAI GRTFRKQESTSFRTVPFVLV+G S YSDF+VVNM+GSRHP
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDGHSTYSDFVVVNMEGSRHP 180

Query: 181 LPLTTVYHQLEPVCATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVPV 240
           LPLTTVYHQL+PVCATPYTFLQA+FGHEYPVGVLDEEKILPLGKNISAVGICSF NGVPV
Sbjct: 181 LPLTTVYHQLQPVCATPYTFLQAVFGHEYPVGVLDEEKILPLGKNISAVGICSFENGVPV 240

Query: 241 IKSCKDFPHFLCEMTKDQMILDLVFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRWT 300
           IKSC DFPHFLCEMTKDQMILDLVFKTK LFWSSIVLGSL+VGILGY+ VRNWN+WK+W 
Sbjct: 241 IKSCSDFPHFLCEMTKDQMILDLVFKTKFLFWSSIVLGSLTVGILGYSAVRNWNRWKQWR 300

Query: 301 QQGRLQDSGNDSTMSQLDSSNNIPIYGDSGTALDDEVAAVSDDEV-LNIPDGQLCVICLM 360
           Q  RLQ+S NDST SQLDSSN+IPIYGD   A DDE A V DDE+  N+PDGQLCVICLM
Sbjct: 301 QHRRLQNSRNDSTTSQLDSSNDIPIYGD--FASDDEAATVPDDELSSNVPDGQLCVICLM 360

Query: 361 RRKRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIHRSVRIYDS 407
           RRKRSAFIPCGHLVCCERCA+SVERDS+PKCP+CRQQI  SVRIYDS
Sbjct: 361 RRKRSAFIPCGHLVCCERCAVSVERDSSPKCPICRQQIRSSVRIYDS 405

BLAST of Lcy05g000880 vs. NCBI nr
Match: KAG7013989.1 (E3 ubiquitin-protein ligase SPL2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 725.7 bits (1872), Expect = 2.2e-205
Identity = 365/408 (89.46%), Postives = 382/408 (93.63%), Query Frame = 0

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSSSE ALVSL+S+FALSFDGAVLGAALAYA VRTVLKLTASCSAL ELRKAPYV VSDL
Sbjct: 1   MSSSEQALVSLLSQFALSFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIISEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RHIIS+GDS QS SD KLVVVRGTVEARSSVEGNWKS+QPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHIISDGDSGQSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFFGWTSDIRAIIGRTFRKQESTSFRTVPFVLV-EGPSPYSDFIVVNMDGSRH 180
           CIFNEWKGFFGWTSD+RAI GRTFRKQESTS RTVPFVLV EG SPYSDF+VVNMDGSRH
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSLRTVPFVLVDEGHSPYSDFVVVNMDGSRH 180

Query: 181 PLPLTTVYHQLEPVCATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVP 240
           PLPLTTVYHQL+PV ATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSF+NGVP
Sbjct: 181 PLPLTTVYHQLQPVSATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFKNGVP 240

Query: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRW 300
           VIKSCKDFPHFLCEMTKDQMILDLVFKTK LFWSS+VLGSLSVG+LGY+VVRNWN+WK+W
Sbjct: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNRWKQW 300

Query: 301 TQQGRLQDSGNDSTMSQLDSSNNIPIYGDSGTALDDEVAAVSDDEV-LNIPDGQLCVICL 360
            QQ RLQDSGNDSTMSQLD SNNIPIYGD GTA D+E AA  +DEV  N+PDGQLCVICL
Sbjct: 301 RQQRRLQDSGNDSTMSQLD-SNNIPIYGDFGTASDNEEAADPEDEVSSNVPDGQLCVICL 360

Query: 361 MRRKRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIHRSVRIYDS 407
           MRRKRSAFIPCGH VCCERCA+SVERDSAPKCPVCRQQI  SVRIYDS
Sbjct: 361 MRRKRSAFIPCGHFVCCERCAMSVERDSAPKCPVCRQQIRSSVRIYDS 407

BLAST of Lcy05g000880 vs. NCBI nr
Match: XP_022964220.1 (E3 ubiquitin-protein ligase SPL2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 725.7 bits (1872), Expect = 2.2e-205
Identity = 360/407 (88.45%), Postives = 378/407 (92.87%), Query Frame = 0

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSSSE ALVSL SRFALSFDGA LGAALAYA  RTVLKLTASCSAL ELRKAP VRVSDL
Sbjct: 1   MSSSEQALVSLFSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60

Query: 61  RHIISEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RH+IS+GDS QS SDQKLVVVRGTVEARS VEGNWKSLQPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHMISDGDSRQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFFGWTSDIRAIIGRTFRKQESTSFRTVPFVLVEGPSPYSDFIVVNMDGSRHP 180
           CIFNEWKGF GWTSD+RAI GRTFRKQESTSFRTVPFVLVEG SPYSDF VVNMDGSRH 
Sbjct: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFAVVNMDGSRHR 180

Query: 181 LPLTTVYHQLEPVCATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVPV 240
           LPLTTVY QL+PVCATPYTFLQALFGHEYPVGVLDEEKILPLG+NIS VG+CSF+NGVP 
Sbjct: 181 LPLTTVYRQLQPVCATPYTFLQALFGHEYPVGVLDEEKILPLGRNISVVGVCSFKNGVPE 240

Query: 241 IKSCKDFPHFLCEMTKDQMILDLVFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRWT 300
           IKSCKDFPHFLCEMTKDQM+LDLVFKTK LFWSSIVLGSLSVG+LG +V+RNWN+WK+W 
Sbjct: 241 IKSCKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR 300

Query: 301 QQGRLQDSGNDSTMSQLDSSNNIPIYGDSGTALDDEVAAVSDDEV-LNIPDGQLCVICLM 360
           QQ RLQDSGNDSTMSQLDSSNNIPIYGD GTA DDEVA++ DDE+  N+PDGQLCVICLM
Sbjct: 301 QQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM 360

Query: 361 RRKRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIHRSVRIYDS 407
           RR+RSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQI  SVRIYDS
Sbjct: 361 RRRRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 407

BLAST of Lcy05g000880 vs. NCBI nr
Match: XP_022992269.1 (E3 ubiquitin-protein ligase SPL2 [Cucurbita maxima] >XP_022992271.1 E3 ubiquitin-protein ligase SPL2 [Cucurbita maxima])

HSP 1 Score: 724.9 bits (1870), Expect = 3.8e-205
Identity = 364/408 (89.22%), Postives = 382/408 (93.63%), Query Frame = 0

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSSSE ALVSL+S+FALSFDGAVLGAALAYA VRTVLKLTASCSAL ELRKAPYV VSDL
Sbjct: 1   MSSSEQALVSLLSQFALSFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIISEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RHIIS+GDS +S SD KLVVVRGTVEARSSVEGNWKS+QPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHIISDGDSGKSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFFGWTSDIRAIIGRTFRKQESTSFRTVPFVLV-EGPSPYSDFIVVNMDGSRH 180
           CIFNEWKGFFGWTSD+RAI GRTFRKQESTSFRTVPFVLV EG SPYSDF+VVNMDGSRH
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDEGHSPYSDFVVVNMDGSRH 180

Query: 181 PLPLTTVYHQLEPVCATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVP 240
           PLPLTTVYHQL+PV ATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSF+NGVP
Sbjct: 181 PLPLTTVYHQLQPVSATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFKNGVP 240

Query: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRW 300
           VIKSCKDFPHFLCEMTKDQMILDLVFKTK LFWSS+VLGSLSVG+LGY+VVRNWN+WK+W
Sbjct: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNRWKQW 300

Query: 301 TQQGRLQDSGNDSTMSQLDSSNNIPIYGDSGTALDDEVAAVSDDEV-LNIPDGQLCVICL 360
            QQ RLQDSGNDSTMSQLD SNNIPIYGD GTA D+E A   +DEV  N+PDGQLCVICL
Sbjct: 301 RQQRRLQDSGNDSTMSQLD-SNNIPIYGDFGTASDNEEAVDPEDEVSSNVPDGQLCVICL 360

Query: 361 MRRKRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIHRSVRIYDS 407
           MRRKRSAFIPCGH VCCERCA+SVERDSAPKCPVCRQQI  SVRIYDS
Sbjct: 361 MRRKRSAFIPCGHFVCCERCAMSVERDSAPKCPVCRQQIRSSVRIYDS 407

BLAST of Lcy05g000880 vs. NCBI nr
Match: XP_023549225.1 (LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase SPL2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 724.2 bits (1868), Expect = 6.4e-205
Identity = 364/408 (89.22%), Postives = 382/408 (93.63%), Query Frame = 0

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSSSE ALVSL+S+FAL FDGAVLGAALAYA VRTVLKLTASCSAL ELRKAPYV VSDL
Sbjct: 1   MSSSEQALVSLLSQFALXFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIISEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RHIIS+GDS QS SD KLVVVRGTVEARSSVEGNWKS+QPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHIISDGDSGQSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFFGWTSDIRAIIGRTFRKQESTSFRTVPFVLV-EGPSPYSDFIVVNMDGSRH 180
           CIFNEWKGFFGWTSD+RAI GRTFRKQESTSFRTVPFVLV EG SPYSDF+VVNMDGSRH
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDEGHSPYSDFVVVNMDGSRH 180

Query: 181 PLPLTTVYHQLEPVCATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVP 240
           PLPLTTVYHQL+PV ATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSF+NGVP
Sbjct: 181 PLPLTTVYHQLQPVSATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFKNGVP 240

Query: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRW 300
           VIKSCKDFPHFLCEMTKDQMILDLVFKTK LFWSS+VLGSLSVG+LGY+VVRNWN+WK+W
Sbjct: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNRWKQW 300

Query: 301 TQQGRLQDSGNDSTMSQLDSSNNIPIYGDSGTALDDEVAAVSDDEV-LNIPDGQLCVICL 360
            QQ RLQDSGNDSTMSQLD SN+IPIYGD GTA D+E AA  +DEV  N+PDGQLCVICL
Sbjct: 301 RQQRRLQDSGNDSTMSQLD-SNDIPIYGDFGTASDNEEAADPEDEVSSNVPDGQLCVICL 360

Query: 361 MRRKRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIHRSVRIYDS 407
           MRRKRSAFIPCGH VCCERCA+SVERDSAPKCPVCRQQI  SVRIYDS
Sbjct: 361 MRRKRSAFIPCGHFVCCERCAMSVERDSAPKCPVCRQQIRSSVRIYDS 407

BLAST of Lcy05g000880 vs. NCBI nr
Match: XP_022954158.1 (E3 ubiquitin-protein ligase SPL2 [Cucurbita moschata] >XP_022954159.1 E3 ubiquitin-protein ligase SPL2 [Cucurbita moschata])

HSP 1 Score: 723.4 bits (1866), Expect = 1.1e-204
Identity = 364/408 (89.22%), Postives = 381/408 (93.38%), Query Frame = 0

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSSSE ALVSL+S+FALSFDGAVLGAALAYA VRTVLKLTASCSAL ELRKAPYV VSDL
Sbjct: 1   MSSSEQALVSLLSQFALSFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHIISEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RHIIS+GDS QS SD KLVVVRGTVEARSSVEGNWKS+QPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHIISDGDSGQSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFFGWTSDIRAIIGRTFRKQESTSFRTVPFVLV-EGPSPYSDFIVVNMDGSRH 180
           CIFNEWKGFFGWTSD+RAI GRTFRKQESTS RTVPFVLV EG SPYSDF+VVNMDGSRH
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSLRTVPFVLVDEGHSPYSDFVVVNMDGSRH 180

Query: 181 PLPLTTVYHQLEPVCATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFRNGVP 240
           PLPLTTVYHQL+PV ATPYTFLQALFGHEYPVGVLDEEKILPLGK ISAVGICSF+NGVP
Sbjct: 181 PLPLTTVYHQLQPVSATPYTFLQALFGHEYPVGVLDEEKILPLGKKISAVGICSFKNGVP 240

Query: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRW 300
           VIKSCKDFPHFLCEMTKDQMILDLVFKTK LFWSS+VLGSLSVG+LGY+VVRNWN+WK+W
Sbjct: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNRWKQW 300

Query: 301 TQQGRLQDSGNDSTMSQLDSSNNIPIYGDSGTALDDEVAAVSDDEV-LNIPDGQLCVICL 360
            QQ RLQDSGNDSTMSQLD SNNIPIYGD GTA D+E AA  +DEV  N+PDGQLCVICL
Sbjct: 301 RQQRRLQDSGNDSTMSQLD-SNNIPIYGDFGTASDNEEAADPEDEVSSNVPDGQLCVICL 360

Query: 361 MRRKRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIHRSVRIYDS 407
           MRRKRSAFIPCGH VCCERCA+SVERDSAPKCPVCRQQI  SVRIYDS
Sbjct: 361 MRRKRSAFIPCGHFVCCERCAMSVERDSAPKCPVCRQQIRSSVRIYDS 407

BLAST of Lcy05g000880 vs. TAIR 10
Match: AT1G54150.1 (E3 Ubiquitin ligase family protein )

HSP 1 Score: 367.9 bits (943), Expect = 1.1e-101
Identity = 204/413 (49.39%), Postives = 274/413 (66.34%), Query Frame = 0

Query: 1   MSSSELALVSLVSRFALSFDGAVLGAALAYATVRTVLKLTASCSALKELRKAPYVRVSDL 60
           MSS E AL++L++  ALSFDGA+LG  LA + V + LK  ++ +ALK+++ AP V +SDL
Sbjct: 1   MSSPERALLNLLTDIALSFDGAILGLTLAVSAVGSALKYASTNAALKKIKDAPEVSISDL 60

Query: 61  RHII------SEGDSEQSGSDQKLVVVRGTVEARSSVEGNWKSLQPNVLISQESGDRAVI 120
           R ++      SE +  +  +DQ++VVVRG V+ + S +  +K+   NVLIS E+GD+A+I
Sbjct: 61  RSLLPASEDKSETNDNRKSNDQRIVVVRGVVKPKISGDEGYKN--NNVLISPETGDKALI 120

Query: 121 IQRTQMCIFNEWKGFFGWTSDIRAIIGRTFRKQESTSFRTVPFVLV-EGPSPYSDFIVVN 180
           IQRTQ  +++ WK  F  T   R ++ R+ RK  +   RTVPFV+V +     S F+ VN
Sbjct: 121 IQRTQTYVYSGWKRLFQSTGH-RFMLERSLRKHGADFTRTVPFVIVGKDQQSNSSFVAVN 180

Query: 181 MDGSRHPLPLTTVYHQLEPVCATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICS 240
           MDGSR PLPLTTVY++L+P+ +   +FLQA    +YPVG+LD EKILP GK+I+AVGI S
Sbjct: 181 MDGSRQPLPLTTVYNRLQPINS---SFLQAFLYPDYPVGLLDIEKILPPGKDITAVGIYS 240

Query: 241 FRNGVPVIKSCKDFPHFLCEMTKDQMILDLVFKTKILFWSSIVLGSLSVGILGYAVVRNW 300
           F NGVP IKSC+D P+FL EMTKD+MI DL+ +T  +F  S++LG +SVGIL YA VR W
Sbjct: 241 FNNGVPEIKSCQDLPYFLSEMTKDKMIEDLMEQTNFIFLGSVILGIVSVGILSYAAVRTW 300

Query: 301 NKWKRWTQQGRLQDSGNDSTMSQLDSSNNIPIYGDSGTALDDEVAAVSDDEVLNIPDGQL 360
           NKWK+W  Q  L    NDS                    +DDE      ++   IPDG+L
Sbjct: 301 NKWKQWNHQRELPQRPNDS-------------------VVDDE-----PEDADEIPDGEL 360

Query: 361 CVICLMRRKRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIHRSVRIYDS 407
           CVIC+ RR+  AFIPCGH+VCC RCA +VER+  PKCPVC Q I  S+R+Y S
Sbjct: 361 CVICVSRRRVPAFIPCGHVVCCRRCASTVERELNPKCPVCLQSIRGSMRVYYS 383

BLAST of Lcy05g000880 vs. TAIR 10
Match: AT1G59560.1 (E3 Ubiquitin ligase family protein )

HSP 1 Score: 64.3 bits (155), Expect = 2.6e-10
Identity = 66/262 (25.19%), Postives = 123/262 (46.95%), Query Frame = 0

Query: 153 RTVPFVLVEGPSPYSDFIVVNMDGSRHPLPL-----TTVYHQLEPVCATPYTFLQALFGH 212
           + VP+ L +G         VN+D S+  L L     + V+ + EPV     + +Q   G+
Sbjct: 107 KEVPWYLDDGTGR------VNVDVSQGELGLALTVGSDVFEKAEPV-----SLVQGALGY 166

Query: 213 EYP---VGVLDEEKILPLGKNISAVGICSFRNGVPVIKSCK--DFPHFLCEMTKDQMILD 272
                 +GV   E+++P+G  ++ VG  + R+G+  ++  K    P ++  +  DQ+I  
Sbjct: 167 LKGFKILGVRHVERVVPIGTPLTVVGE-AVRDGMGNVRIQKPEQGPFYVTYIPLDQLISK 226

Query: 273 LVFKTKILFWSSIVLGSLSVGILGYAVVRNWNKWKRWTQQGRLQDSGNDSTMSQLDSSNN 332
           L   ++   ++S+ L  L V ++   V+    K    T + R +       +    +   
Sbjct: 227 LGDLSRRFKYASMGLTVLGVILISKPVIEYILKRIEDTLERRRRQFALKRVVDAA-ARRA 286

Query: 333 IPIYGDSGTALDDEVAAVSDDEVLNIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISV 392
            P+ G  GT+ D +            PD  LCV+CL ++  +AF+ CGH+ CC  C++ +
Sbjct: 287 KPVTG-GGTSRDGDT-----------PD--LCVVCLDQKYNTAFVECGHMCCCTPCSLQL 336

Query: 393 ERDSAPKCPVCRQQIHRSVRIY 405
                  CP+CR++I + ++IY
Sbjct: 347 R-----TCPLCRERIQQVLKIY 336

BLAST of Lcy05g000880 vs. TAIR 10
Match: AT4G24204.1 (RING/U-box superfamily protein )

HSP 1 Score: 55.1 bits (131), Expect = 1.6e-07
Identity = 25/70 (35.71%), Postives = 38/70 (54.29%), Query Frame = 0

Query: 334 DDEVAAVSDDEVLNIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISVERDSAPKCPVC 393
           D E A+ S  +  ++    LCVIC   R+   F+PCGH   C  CA  +  + +  CP+C
Sbjct: 51  DVETASFSSSD--DVDYSTLCVICFEERRNCFFVPCGHSATCRGCAQRILSEESKVCPIC 110

Query: 394 RQQIHRSVRI 404
           R+ I +S R+
Sbjct: 111 RRVIRKSKRL 118

BLAST of Lcy05g000880 vs. TAIR 10
Match: AT4G24204.2 (RING/U-box superfamily protein )

HSP 1 Score: 55.1 bits (131), Expect = 1.6e-07
Identity = 25/70 (35.71%), Postives = 38/70 (54.29%), Query Frame = 0

Query: 334 DDEVAAVSDDEVLNIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISVERDSAPKCPVC 393
           D E A+ S  +  ++    LCVIC   R+   F+PCGH   C  CA  +  + +  CP+C
Sbjct: 85  DVETASFSSSD--DVDYSTLCVICFEERRNCFFVPCGHSATCRGCAQRILSEESKVCPIC 144

Query: 394 RQQIHRSVRI 404
           R+ I +S R+
Sbjct: 145 RRVIRKSKRL 152

BLAST of Lcy05g000880 vs. TAIR 10
Match: AT4G24204.3 (RING/U-box superfamily protein )

HSP 1 Score: 55.1 bits (131), Expect = 1.6e-07
Identity = 25/70 (35.71%), Postives = 38/70 (54.29%), Query Frame = 0

Query: 334 DDEVAAVSDDEVLNIPDGQLCVICLMRRKRSAFIPCGHLVCCERCAISVERDSAPKCPVC 393
           D E A+ S  +  ++    LCVIC   R+   F+PCGH   C  CA  +  + +  CP+C
Sbjct: 106 DVETASFSSSD--DVDYSTLCVICFEERRNCFFVPCGHSATCRGCAQRILSEESKVCPIC 165

Query: 394 RQQIHRSVRI 404
           R+ I +S R+
Sbjct: 166 RRVIRKSKRL 173

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SYH31.5e-10049.39E3 ubiquitin-protein ligase SPL2 OS=Arabidopsis thaliana OX=3702 GN=SPL2 PE=2 SV... [more]
Q94HV73.6e-0925.19E3 ubiquitin-protein ligase SPL1 OS=Arabidopsis thaliana OX=3702 GN=SPL1 PE=2 SV... [more]
P414371.5e-0745.61E3 ubiquitin-protein ligase IAP-3 OS=Orgyia pseudotsugata multicapsid polyhedros... [more]
O088637.5e-0736.62Baculoviral IAP repeat-containing protein 3 OS=Mus musculus OX=10090 GN=Birc3 PE... [more]
Q243067.5e-0743.33Death-associated inhibitor of apoptosis 1 OS=Drosophila melanogaster OX=7227 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1HK841.1e-20588.45RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111464304... [more]
A0A6J1JT391.8e-20589.22RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488642 P... [more]
A0A6J1GS485.3e-20589.22RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111456504... [more]
A0A6J1KHU79.0e-20587.96RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111494539 P... [more]
A0A5D3BWQ08.4e-20386.98RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
KAG7013989.12.2e-20589.46E3 ubiquitin-protein ligase SPL2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022964220.12.2e-20588.45E3 ubiquitin-protein ligase SPL2-like isoform X2 [Cucurbita moschata][more]
XP_022992269.13.8e-20589.22E3 ubiquitin-protein ligase SPL2 [Cucurbita maxima] >XP_022992271.1 E3 ubiquitin... [more]
XP_023549225.16.4e-20589.22LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase SPL2 [Cucurbita pepo subsp. pep... [more]
XP_022954158.11.1e-20489.22E3 ubiquitin-protein ligase SPL2 [Cucurbita moschata] >XP_022954159.1 E3 ubiquit... [more]
Match NameE-valueIdentityDescription
AT1G54150.11.1e-10149.39E3 Ubiquitin ligase family protein [more]
AT1G59560.12.6e-1025.19E3 Ubiquitin ligase family protein [more]
AT4G24204.11.6e-0735.71RING/U-box superfamily protein [more]
AT4G24204.21.6e-0735.71RING/U-box superfamily protein [more]
AT4G24204.31.6e-0735.71RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 354..393
e-value: 0.0029
score: 26.8
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 354..394
score: 10.851695
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 333..406
e-value: 1.4E-16
score: 61.7
IPR022170E3 Ubiquitin ligase MUL1-likePFAMPF12483GIDEcoord: 145..284
e-value: 3.9E-34
score: 117.4
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 352..399
e-value: 1.1E-13
score: 50.8
NoneNo IPR availablePANTHERPTHR47355:SF1E3 UBIQUITIN-PROTEIN LIGASE SPL2coord: 1..406
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 348..400
IPR044247E3 ubiquitin-protein ligase SPL2-likePANTHERPTHR47355E3 UBIQUITIN-PROTEIN LIGASE SPL2coord: 1..406

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy05g000880.1Lcy05g000880.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006996 organelle organization
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016746 acyltransferase activity
molecular_function GO:0016874 ligase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004842 ubiquitin-protein transferase activity