Lcy05g000810 (gene) Sponge gourd (P93075) v1

Overview
NameLcy05g000810
Typegene
OrganismLuffa cylindrica cv. P93075 (Sponge gourd (P93075) v1)
DescriptionLipoxygenase
LocationChr05: 843513 .. 846541 (+)
RNA-Seq ExpressionLcy05g000810
SyntenyLcy05g000810
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACTAGCAAATGAAATTATCGGATCAACTTTTCTTCACAAATCATCGTTGGCTTCCCAGTCTAAATTGTTTCAAACTGGTAGTTTTGAGAAGCAGTTCAGGCCTATTTGGGCTGTTCCAGTAGAGAAAAGGAAGGTGGTGCATTTAAGGAAGGCTGTGAACGGCCCTGTGGCTGCAATCAGTGAGGATTTGGTTAAGGCTGTGCCCTTGGCCGAGAAGCCGGTCAAGTTCAAGGTCAGAGCCGTTGTGACTATAAGAAACAAGAACAAGGAAGACATTAAAGAGACGATTGTTAAGCATTTGGATGCTTTCACTGATAGGATTGGCCAGAACGTTGTTCTCCAACTTATCAGCACTGAGATTGACCCAAGTGAGTCATTTATCTTTTTCCCCTTTTTTTTTTTTTTTTTGTTGTTTTTGTTTTATTTTTGGTTTTTTCTCTTTCTGGGTGTTCTATATTCAAGGTTTCCTCTTCTGGGTATTTTTTTCATTTTCATGTTTTCGTTGTTGTTTTTGTGAGAAAATCCCTGACTAGAGAGTAGATTTGTATAATTTTGTCTGAATCTTATAGAGTTTTACTGAATTGGTTTTGATTTGGATCATTGAAGAAACAAACGCTCCAAAGAAAAGCAACGAAGCCGTGTTGAAAGATTGGTCAAAGAAAACTAATCTAAAAGCTGAGAGGGTAAATTATACAGCAGAGTTTTTACTGACCTCGGACTTCGGAGAGCTAGGAGCGATCACAATTACCAACAAGCACCAGCAGGAATTCTTCTTGGAGACTATCACCATCGAGCAATTTGCAAGTGATCCTATTCATTTTCCCTGCAATTCTTGGGTTCAGTCCAGAAAAGATCACCCTGCAAAAAGGATATTTTTCTCTAATAAGGTGAGTTTTTTTTTTCCTTTTTTCTTTTTAACCTTTTTTTTTTTCTTCTCAGTTCAGCTTTTGTAATTTGTCCTCAACTAATAATAAAGACCTTTATGAGATAATGCGAGTTAAAGCTGATGTGATGTATTCTTTCATATTTCTACTTTTAAATGACTCCACGTCCTCTTGTCATTGTAATGAATGACAATATAATTCTATTATCTTGACCATTGGGAGGTGAAAAGAAAAGGAGGAAACATTTCTTTTCTGTTTAAAATTTTCCTTTATTTCTTTCATTCCTTTCTACAGTCCTGAATCTGCTAAACTATTTTATAAAGATTGCCCGTGGACCCAGAATTCAGCCTACCTAACTAACTACAGTTGAATCATTCAAGTTTTCTTCTGTTGCTATTATGAGCATGCCAATGATGAATGAGAAAAAACAATTTCAAACCAAGAACGGCTAGAGATTATAAACGAACCTTCCTACAAAATAACGTATTCTCTTTCAATCTTGATCTGGATTTTTGAATGGCTAGCTGATGTGTTATACAATTCTGCAGCCTTATCTACCAGGTGAGACACCTGCAGGGATTAAAGCATTAAGAGAGAAAGAGCTTAAAGACATTAGAGGTGATGGAAGAGGAGAGAGAAAACTGTCTGATAGAGTATATGACTTTGATGTGTACAACGACTTGGGAAATCCAGACAAGGGAATAGAATTTGCACGCCCAAGGCTTGGTGGCGAGAAAGTTCCATATCCTAGACGGTGTCGCACGGGACGTCCGCCTAGCGATACTGGTAAAATCCGACACCATCATTTGCAATTATAACGATGAATTGGCAAAGGCTATGTAAATCCATATCTAATAATTGTATATGCTACAGATATAACTGCAGAGAGTCGAGTCGAGAAACCGTTACCCATGTACGTGCCAAGAGATGAACAATTTGAGGAGTCTAAGCAAACCACTTTCTCTCTTGGGAGACTTAAGGCAGTTCTTCACAACTTAATTCCATCTCTTAAAGCCAGCATTCTGTCAAATAAACATGATTTCCATGGGTTTTCAGACATTGACAGCCTTTACAGTGAAGGGGTGCTCCTTAAATTAGGCTTGCAAGATGAACTCCTGAAGAAGCTCCCACTGCCAAGAGTTGTGAGTGAATCCAGCCAAGGACTGCTAAGATACAATACACCTAAGATCCTTTCAAGTAAGTGACATCATCAGCCTTCAAAAGTATTATTCATATATTCATGAAATTGGCTAGCAACCTGCAGTTTCCAAAAACCTCACCTAATTAGATAATCTAGATGGCCTAAATTACTTTTCTAAGATTCAAAACAACTGTTTTACAGAATTAGAACAATCCCAATTAATGAAATAGAAAAGTCTTTTCAACTTTGGTAACCTCACAAGTTACTTTCATAGATAGGCATTGGCATACCACATCAGTTTTCGCTTGAATGCAAATTGACAAACCCAACATTCAGAAATCTTGATCATAATCAATGTATACGTCATGTCCAAATTGCAGAGGACAAGTTTGCCTGGCTGCGAGATGACGAATTTGCCCGCCAAGCAATAGCAGGAATTAACCCAGTCAACATTGAGAGGCTCAAGGTTTTCCCTCCAGTAAGCAACCTTGATCCTGACGTTTATGGCCCACAGGAATCTGCCCTTAAAGAAGAACACATTCTTGGCCAACTCAATGGCATGACCGTGCAGCAGGTAAATAATCACATCAAGATGTTAACCCATCACTCTGATTTTTCTTTTTCCCACTAAAATGACTACCACATTTGCAAATCCTAACATTATCTCGCCTCAAATTGCTTTGATCGTTATAGGCATTGGATGAAAACAAATTGTTCATAGTGGACTACCATGATGTTTACCTTCCGTTTATTGATCGGATCAACGCTCTCGACGGCCGGAAAACATATGCAACTCGCACAATCTTCTTCTTGACTCCACTTGGAACTCTGAAACCTATCGCCATAGAGCTCAGCCTTCCACCAACCGGACCAAGTTCTCGATCGAAGCGCATCGTAACTCCACCCGTCGACGCTACCAGCAACTGGATATGGCAGCTCGCCAAGGCTCACGTCTGCTCCAACGACGCCGGAGTTCATCAACTAGTTAACCACTGGTAA

mRNA sequence

ATGGCACTAGCAAATGAAATTATCGGATCAACTTTTCTTCACAAATCATCGTTGGCTTCCCAGTCTAAATTGTTTCAAACTGGTAGTTTTGAGAAGCAGTTCAGGCCTATTTGGGCTGTTCCAGTAGAGAAAAGGAAGGTGGTGCATTTAAGGAAGGCTGTGAACGGCCCTGTGGCTGCAATCAGTGAGGATTTGGTTAAGGCTGTGCCCTTGGCCGAGAAGCCGGTCAAGTTCAAGGTCAGAGCCGTTGTGACTATAAGAAACAAGAACAAGGAAGACATTAAAGAGACGATTGTTAAGCATTTGGATGCTTTCACTGATAGGATTGGCCAGAACGTTGTTCTCCAACTTATCAGCACTGAGATTGACCCAAAAACAAACGCTCCAAAGAAAAGCAACGAAGCCGTGTTGAAAGATTGGTCAAAGAAAACTAATCTAAAAGCTGAGAGGGTAAATTATACAGCAGAGTTTTTACTGACCTCGGACTTCGGAGAGCTAGGAGCGATCACAATTACCAACAAGCACCAGCAGGAATTCTTCTTGGAGACTATCACCATCGAGCAATTTGCAAGTGATCCTATTCATTTTCCCTGCAATTCTTGGGTTCAGTCCAGAAAAGATCACCCTGCAAAAAGGATATTTTTCTCTAATAAGCCTTATCTACCAGGTGAGACACCTGCAGGGATTAAAGCATTAAGAGAGAAAGAGCTTAAAGACATTAGAGGTGATGGAAGAGGAGAGAGAAAACTGTCTGATAGAGTATATGACTTTGATGTGTACAACGACTTGGGAAATCCAGACAAGGGAATAGAATTTGCACGCCCAAGGCTTGGTGGCGAGAAAGTTCCATATCCTAGACGGTGTCGCACGGGACGTCCGCCTAGCGATACTGATATAACTGCAGAGAGTCGAGTCGAGAAACCGTTACCCATGTACGTGCCAAGAGATGAACAATTTGAGGAGTCTAAGCAAACCACTTTCTCTCTTGGGAGACTTAAGGCAGTTCTTCACAACTTAATTCCATCTCTTAAAGCCAGCATTCTGTCAAATAAACATGATTTCCATGGGTTTTCAGACATTGACAGCCTTTACAGTGAAGGGGTGCTCCTTAAATTAGGCTTGCAAGATGAACTCCTGAAGAAGCTCCCACTGCCAAGAGTTGTGAGTGAATCCAGCCAAGGACTGCTAAGATACAATACACCTAAGATCCTTTCAAAGGACAAGTTTGCCTGGCTGCGAGATGACGAATTTGCCCGCCAAGCAATAGCAGGAATTAACCCAGTCAACATTGAGAGGCTCAAGGTTTTCCCTCCAGTAAGCAACCTTGATCCTGACGTTTATGGCCCACAGGAATCTGCCCTTAAAGAAGAACACATTCTTGGCCAACTCAATGGCATGACCGTGCAGCAGGCATTGGATGAAAACAAATTGTTCATAGTGGACTACCATGATGTTTACCTTCCGTTTATTGATCGGATCAACGCTCTCGACGGCCGGAAAACATATGCAACTCGCACAATCTTCTTCTTGACTCCACTTGGAACTCTGAAACCTATCGCCATAGAGCTCAGCCTTCCACCAACCGGACCAAGTTCTCGATCGAAGCGCATCGTAACTCCACCCGTCGACGCTACCAGCAACTGGATATGGCAGCTCGCCAAGGCTCACGTCTGCTCCAACGACGCCGGAGTTCATCAACTAGTTAACCACTGGTAA

Coding sequence (CDS)

ATGGCACTAGCAAATGAAATTATCGGATCAACTTTTCTTCACAAATCATCGTTGGCTTCCCAGTCTAAATTGTTTCAAACTGGTAGTTTTGAGAAGCAGTTCAGGCCTATTTGGGCTGTTCCAGTAGAGAAAAGGAAGGTGGTGCATTTAAGGAAGGCTGTGAACGGCCCTGTGGCTGCAATCAGTGAGGATTTGGTTAAGGCTGTGCCCTTGGCCGAGAAGCCGGTCAAGTTCAAGGTCAGAGCCGTTGTGACTATAAGAAACAAGAACAAGGAAGACATTAAAGAGACGATTGTTAAGCATTTGGATGCTTTCACTGATAGGATTGGCCAGAACGTTGTTCTCCAACTTATCAGCACTGAGATTGACCCAAAAACAAACGCTCCAAAGAAAAGCAACGAAGCCGTGTTGAAAGATTGGTCAAAGAAAACTAATCTAAAAGCTGAGAGGGTAAATTATACAGCAGAGTTTTTACTGACCTCGGACTTCGGAGAGCTAGGAGCGATCACAATTACCAACAAGCACCAGCAGGAATTCTTCTTGGAGACTATCACCATCGAGCAATTTGCAAGTGATCCTATTCATTTTCCCTGCAATTCTTGGGTTCAGTCCAGAAAAGATCACCCTGCAAAAAGGATATTTTTCTCTAATAAGCCTTATCTACCAGGTGAGACACCTGCAGGGATTAAAGCATTAAGAGAGAAAGAGCTTAAAGACATTAGAGGTGATGGAAGAGGAGAGAGAAAACTGTCTGATAGAGTATATGACTTTGATGTGTACAACGACTTGGGAAATCCAGACAAGGGAATAGAATTTGCACGCCCAAGGCTTGGTGGCGAGAAAGTTCCATATCCTAGACGGTGTCGCACGGGACGTCCGCCTAGCGATACTGATATAACTGCAGAGAGTCGAGTCGAGAAACCGTTACCCATGTACGTGCCAAGAGATGAACAATTTGAGGAGTCTAAGCAAACCACTTTCTCTCTTGGGAGACTTAAGGCAGTTCTTCACAACTTAATTCCATCTCTTAAAGCCAGCATTCTGTCAAATAAACATGATTTCCATGGGTTTTCAGACATTGACAGCCTTTACAGTGAAGGGGTGCTCCTTAAATTAGGCTTGCAAGATGAACTCCTGAAGAAGCTCCCACTGCCAAGAGTTGTGAGTGAATCCAGCCAAGGACTGCTAAGATACAATACACCTAAGATCCTTTCAAAGGACAAGTTTGCCTGGCTGCGAGATGACGAATTTGCCCGCCAAGCAATAGCAGGAATTAACCCAGTCAACATTGAGAGGCTCAAGGTTTTCCCTCCAGTAAGCAACCTTGATCCTGACGTTTATGGCCCACAGGAATCTGCCCTTAAAGAAGAACACATTCTTGGCCAACTCAATGGCATGACCGTGCAGCAGGCATTGGATGAAAACAAATTGTTCATAGTGGACTACCATGATGTTTACCTTCCGTTTATTGATCGGATCAACGCTCTCGACGGCCGGAAAACATATGCAACTCGCACAATCTTCTTCTTGACTCCACTTGGAACTCTGAAACCTATCGCCATAGAGCTCAGCCTTCCACCAACCGGACCAAGTTCTCGATCGAAGCGCATCGTAACTCCACCCGTCGACGCTACCAGCAACTGGATATGGCAGCTCGCCAAGGCTCACGTCTGCTCCAACGACGCCGGAGTTCATCAACTAGTTAACCACTGGTAA

Protein sequence

MALANEIIGSTFLHKSSLASQSKLFQTGSFEKQFRPIWAVPVEKRKVVHLRKAVNGPVAAISEDLVKAVPLAEKPVKFKVRAVVTIRNKNKEDIKETIVKHLDAFTDRIGQNVVLQLISTEIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNYTAEFLLTSDFGELGAITITNKHQQEFFLETITIEQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIKALREKELKDIRGDGRGERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEKVPYPRRCRTGRPPSDTDITAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASILSNKHDFHGFSDIDSLYSEGVLLKLGLQDELLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFARQAIAGINPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDENKLFIVDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLPPTGPSSRSKRIVTPPVDATSNWIWQLAKAHVCSNDAGVHQLVNHW
Homology
BLAST of Lcy05g000810 vs. ExPASy Swiss-Prot
Match: O24371 (Linoleate 13S-lipoxygenase 3-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LOX3.1 PE=1 SV=1)

HSP 1 Score: 872.5 bits (2253), Expect = 2.7e-252
Identity = 431/577 (74.70%), Postives = 499/577 (86.48%), Query Frame = 0

Query: 1   MALANEIIGSTFLHKSSL---ASQSKLFQTGSFEKQFRPIWAVPVEKRKVVHLRKAV-NG 60
           MALA EI+G + L KSS    +S   LF   ++ K+    +    + R+ +  RKA    
Sbjct: 1   MALAKEIMGISLLEKSSSFMNSSSMALFNPNNYHKENHLWFNQQFQGRRNLSRRKAFRQS 60

Query: 61  PVAAISEDLVKAVPLAEKPVKFKVRAVVTIRNKNKEDIKETIVKHLDAFTDRIGQNVVLQ 120
            +AAISE+L+K VP  EK V+FKVRAVVT+RNKNKED+KETIVKHLDAFTD+IG+NV L+
Sbjct: 61  TMAAISENLIKVVP--EKAVRFKVRAVVTVRNKNKEDLKETIVKHLDAFTDKIGRNVTLE 120

Query: 121 LISTEIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNYTAEFLLTSDFGELGAITITNKHQ 180
           LIST++DP T  PKKSN+AVLKDWSKK+NLK ERVNYTAEF++ S+FG  GAIT+TNKHQ
Sbjct: 121 LISTDMDPNTKGPKKSNQAVLKDWSKKSNLKTERVNYTAEFIVDSNFGNPGAITVTNKHQ 180

Query: 181 QEFFLETITIEQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIKALREKE 240
           QEFFLE+ITIE FA  P+HFPCNSWVQ +KDHP KRIFFSN+PYLP ETPAG+K+LRE+E
Sbjct: 181 QEFFLESITIEGFACGPVHFPCNSWVQPKKDHPGKRIFFSNQPYLPDETPAGLKSLRERE 240

Query: 241 LKDIRGDGRGERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGG-EKVPYPRRCRTGRPPS 300
           L+D+RGDG+G RKLSDR+YD+D+YNDLGNPDKGI+FARP+LGG + VPYPRRCR+GR P+
Sbjct: 241 LRDLRGDGKGVRKLSDRIYDYDIYNDLGNPDKGIDFARPKLGGDDNVPYPRRCRSGRVPT 300

Query: 301 DTDITAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASILSNKHDFH 360
           DTDI+AESRVEKP P YVPRDEQFEESK  TFS  RLKAVLHNLIPSL ASI SN HDF 
Sbjct: 301 DTDISAESRVEKPNPTYVPRDEQFEESKMNTFSTSRLKAVLHNLIPSLMASISSNNHDFK 360

Query: 361 GFSDIDSLYSEGVLLKLGLQDELLKKLPLPRVVSESSQG-LLRYNTPKILSKDKFAWLRD 420
           GFSDID+LYS+G+LLKLGLQDE+LKKLPLP+VVS   +G LL+Y+TPKILSKDKFAWLRD
Sbjct: 361 GFSDIDNLYSKGLLLKLGLQDEVLKKLPLPKVVSSIKEGDLLKYDTPKILSKDKFAWLRD 420

Query: 421 DEFARQAIAGINPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDE 480
           DEFARQAIAG+NPV+IE+L+ FPPVS LDP++YGPQESALKEEHILG LNGMTVQ+ALD 
Sbjct: 421 DEFARQAIAGVNPVSIEKLQFFPPVSKLDPEIYGPQESALKEEHILGHLNGMTVQEALDA 480

Query: 481 NKLFIVDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLPPTGPSSRS 540
           NKLFIVD+HDVYLPF+DRINALDGRK YATRTIFFL+ +GTLKPIAIELSLP TGPSSRS
Sbjct: 481 NKLFIVDHHDVYLPFLDRINALDGRKAYATRTIFFLSDVGTLKPIAIELSLPQTGPSSRS 540

Query: 541 KRIVTPPVDATSNWIWQLAKAHVCSNDAGVHQLVNHW 572
           KR+VTPPV AT NW WQ+AKAHVC+NDAGVHQLVNHW
Sbjct: 541 KRVVTPPVCATGNWTWQIAKAHVCANDAGVHQLVNHW 575

BLAST of Lcy05g000810 vs. ExPASy Swiss-Prot
Match: Q9LNR3 (Lipoxygenase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX3 PE=2 SV=1)

HSP 1 Score: 810.8 bits (2093), Expect = 9.6e-234
Identity = 405/588 (68.88%), Postives = 482/588 (81.97%), Query Frame = 0

Query: 1   MALANEIIGSTFL-HKSSLASQSKLFQTGSFEKQFRPIWAVPVEKRKVVHLRKAVNGPVA 60
           MALA E++G   +  +SSL S +  F+  +   QF      P ++R     RK  +G VA
Sbjct: 1   MALAKELMGYPLITERSSLVSSASHFKKRTQSTQFS---INPFDRRP----RKTKSGVVA 60

Query: 61  AISEDLVKAVPLA------------EKPVKFKVRAVVTIRNKNKEDIKETIVKHLDAFTD 120
           AISEDLVK +  +            +  VKFKVRAVVT+RNKNKED+KET+VKHLDAF D
Sbjct: 61  AISEDLVKTLRFSTTTGDRKSEEEEKAAVKFKVRAVVTVRNKNKEDLKETLVKHLDAFAD 120

Query: 121 RIGQNVVLQLISTEIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNYTAEFLLTSDFGELG 180
           +IG+N+VL+LIST++DPKT  PKKSN AVLKDWSKK+  KAERV+YTAEF + + FG  G
Sbjct: 121 KIGRNIVLELISTQLDPKTKLPKKSNAAVLKDWSKKSKTKAERVHYTAEFTVDAAFGSPG 180

Query: 181 AITITNKHQQEFFLETITIEQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPA 240
           AIT+ NKHQ+EFFLE+ITIE FA  P+HFPCNSWVQS+KDHP KRIFF+N+PYLP ETP+
Sbjct: 181 AITVMNKHQKEFFLESITIEGFALGPVHFPCNSWVQSQKDHPDKRIFFTNQPYLPNETPS 240

Query: 241 GIKALREKELKDIRGDGRGERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEKVPYPRR 300
           G++ LREKELK++RGDG G RKLSDR+YDFDVYNDLGNPDK  E +RP+LGG++VPYPRR
Sbjct: 241 GLRVLREKELKNLRGDGSGVRKLSDRIYDFDVYNDLGNPDKSSELSRPKLGGKEVPYPRR 300

Query: 301 CRTGRPPSDTDITAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASI 360
           CRTGR  + +D  AESRVEKPLPMYVPRDEQFEESKQ TF+ GRLKAVLH+LIPSLKASI
Sbjct: 301 CRTGRQSTVSDKDAESRVEKPLPMYVPRDEQFEESKQDTFAAGRLKAVLHHLIPSLKASI 360

Query: 361 LSNKHDFHGFSDIDSLYSEGVLLKLGLQDELLKKLPLPRVV----SESSQGLLRYNTPKI 420
           ++   DF  F +ID LY EG+LLKLG QD++ KK PLP+VV     ES++GLL+Y+TPKI
Sbjct: 361 VA--EDFADFGEIDRLYKEGLLLKLGFQDDIFKKFPLPKVVVDTLQESTKGLLKYDTPKI 420

Query: 421 LSKDKFAWLRDDEFARQAIAGINPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQL 480
           LSKDK AWLRDDEFARQAIAGINPVNIER+K FPPVSNLDP +YGPQ SAL ++HI+G L
Sbjct: 421 LSKDKNAWLRDDEFARQAIAGINPVNIERVKTFPPVSNLDPKIYGPQHSALTDDHIIGHL 480

Query: 481 NGMTVQQALDENKLFIVDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIEL 540
           +G +VQQAL+EN+L+++DYHD++LPF+DRINALDGRK YATRTIFFLT LGTLKP+AIEL
Sbjct: 481 DGFSVQQALEENRLYMLDYHDIFLPFLDRINALDGRKAYATRTIFFLTRLGTLKPVAIEL 540

Query: 541 SLPPTGPSSRSKRIVTPPVDATSNWIWQLAKAHVCSNDAGVHQLVNHW 572
           SLPP GP  RSKR++TPPVDATSNW+WQLAKAHV SNDAGVHQLVNHW
Sbjct: 541 SLPPHGPKHRSKRVLTPPVDATSNWMWQLAKAHVSSNDAGVHQLVNHW 579

BLAST of Lcy05g000810 vs. ExPASy Swiss-Prot
Match: Q9FNX8 (Lipoxygenase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX4 PE=2 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 2.6e-231
Identity = 408/592 (68.92%), Postives = 488/592 (82.43%), Query Frame = 0

Query: 1   MALANEIIGSTFLHKSSLASQSKLFQTGSFEKQF-RPIWAV-PVEKRKVVHLRKAVN--G 60
           MALANEI+GS  + + S +  S      S +K+  R  +++ P + R +    +AVN  G
Sbjct: 1   MALANEIMGSRLIFERSSSLASPFHSRFSIKKKTQRTQFSINPFDPRPM----RAVNSSG 60

Query: 61  PVAAISEDLVKAVPLA------------EKPVKFKVRAVVTIRNKNKEDIKETIVKHLDA 120
            VAAISEDLVK + ++            EK VKFKVRAV T+RNKNKED KET+VKHLDA
Sbjct: 61  VVAAISEDLVKTLRISTVGRKQEKEEEEEKSVKFKVRAVATVRNKNKEDFKETLVKHLDA 120

Query: 121 FTDRIGQNVVLQLISTEIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNYTAEFLLTSDFG 180
           FTD+IG+NVVL+L+ST++DPKTN PKKS  AVLKDWSKK+N KAERV+YTAEF + S FG
Sbjct: 121 FTDKIGRNVVLELMSTQVDPKTNEPKKSKAAVLKDWSKKSNSKAERVHYTAEFTVDSAFG 180

Query: 181 ELGAITITNKHQQEFFLETITIEQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGE 240
             GAIT+TNKHQ+EFFLE+ITIE FA  P+HFPCNSWVQS+KDHP+KRI F+N+PYLP E
Sbjct: 181 SPGAITVTNKHQKEFFLESITIEGFACGPVHFPCNSWVQSQKDHPSKRILFTNQPYLPSE 240

Query: 241 TPAGIKALREKELKDIRGDGRGERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEKVPY 300
           TP+G++ LREKEL+++RG+G+GERKLSDR+YD+DVYND+GNPD   E ARP LGG + PY
Sbjct: 241 TPSGLRTLREKELENLRGNGKGERKLSDRIYDYDVYNDIGNPDISRELARPTLGGREFPY 300

Query: 301 PRRCRTGRPPSDTDITAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLK 360
           PRRCRTGR  +DTD+ +E RVEKPLPMYVPRDEQFEESKQ TF+  RLKAVLHNLIPSLK
Sbjct: 301 PRRCRTGRSSTDTDMMSERRVEKPLPMYVPRDEQFEESKQNTFAACRLKAVLHNLIPSLK 360

Query: 361 ASILSNKHDFHGFSDIDSLYSEGVLLKLGLQDELLKKLPLPRVVS---ESSQGLLRYNTP 420
           ASIL+   DF  F +IDSLY EG+LLKLG QD++ KK PLP++V+   +SS+GLLRY+TP
Sbjct: 361 ASILA--EDFANFGEIDSLYKEGLLLKLGFQDDMFKKFPLPKIVTTLQKSSEGLLRYDTP 420

Query: 421 KILSKDKFAWLRDDEFARQAIAGINPVNIERLKVFPPVSNLDPDVYGP-QESALKEEHIL 480
           KI+SKDK+AWLRDDEFARQAIAGINPVNIER+  +PPVSNLDP++YGP   SAL E+HI+
Sbjct: 421 KIVSKDKYAWLRDDEFARQAIAGINPVNIERVTSYPPVSNLDPEIYGPGLHSALTEDHII 480

Query: 481 GQLNGMTVQQALDENKLFIVDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIA 540
           GQL+G+TVQQAL+ N+LF+VDYHD+YLPF+DRINALDGRK YATRTI FLT LGTLKPIA
Sbjct: 481 GQLDGLTVQQALETNRLFMVDYHDIYLPFLDRINALDGRKAYATRTILFLTRLGTLKPIA 540

Query: 541 IELSLPPTGPSS-RSKRIVTPPVDATSNWIWQLAKAHVCSNDAGVHQLVNHW 572
           IELSLP    S+ +SKR+VTPPVDATSNW+WQLAKAHV SNDAGVHQLVNHW
Sbjct: 541 IELSLPSQSSSNQKSKRVVTPPVDATSNWMWQLAKAHVGSNDAGVHQLVNHW 586

BLAST of Lcy05g000810 vs. ExPASy Swiss-Prot
Match: R9WTS6 (Lipoxygenase 1, chloroplastic OS=Tanacetum cinerariifolium OX=118510 GN=LOX1 PE=1 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 3.5e-228
Identity = 402/578 (69.55%), Postives = 473/578 (81.83%), Query Frame = 0

Query: 1   MALANEIIGSTFLHKSSLASQSKLFQTGSFEKQFRPIWAVPVEKRKVVHLRKAVNGPVAA 60
           MALA +I+G++ + + +    S L        + R      +  RK    RK  +  VAA
Sbjct: 1   MALAKQIMGASLMDQKTSVFGSNLCLNHVLVNKHR------LRLRKT---RKNGSMVVAA 60

Query: 61  ISEDLVKAVPL-AEKPVKFKVRAVVTIRNKNKED-IKETIVKHLDAFTDRIGQNVVLQLI 120
           ISEDLVK   +  EKPV FKVRAV+T+RNKNKED  K+TI + +DA TD+IG NVV+QL 
Sbjct: 61  ISEDLVKLXRVEKEKPVTFKVRAVLTVRNKNKEDFFKDTIFRKIDAITDQIGWNVVIQLF 120

Query: 121 STEIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNYTAEFLLTSDFGELGAITITNKHQQE 180
           S +IDP+T A KKSNEAVLKDWSKK+N+K ERVNYTA+ ++ SDFG  GAITI+NKHQ+E
Sbjct: 121 SNDIDPRTRAAKKSNEAVLKDWSKKSNVKTERVNYTADIMVDSDFGIPGAITISNKHQKE 180

Query: 181 FFLETITIEQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIKALREKELK 240
           FFLETITIE FA  P+HFPCNSWVQS KD P  RIFF+N+PYLP ETP G+K+LR +ELK
Sbjct: 181 FFLETITIEGFACGPVHFPCNSWVQSTKDLPNPRIFFTNQPYLPDETPVGLKSLRYQELK 240

Query: 241 DIRGDGRGERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEKVPYPRRCRTGRPPSDTD 300
           D+RGDG G RKLSDR+YD+DVYNDLGNPD+G +F RP LGGEK+PYPRRCRTGR PSDTD
Sbjct: 241 DLRGDGTGVRKLSDRIYDYDVYNDLGNPDRGNDFVRPTLGGEKIPYPRRCRTGRVPSDTD 300

Query: 301 ITAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASILSNKHDFHGFS 360
           ITAESRVEKP P+YVPRDEQFEESK   FS GRL+AVLHNL+PS+  SI S K+DF GFS
Sbjct: 301 ITAESRVEKPFPLYVPRDEQFEESKANAFSTGRLRAVLHNLLPSMVTSI-SKKNDFKGFS 360

Query: 361 DIDSLYSEGVLLKLGLQDELLKKLPLPRVVS---ESSQ--GLLRYNTPKILSKDKFAWLR 420
            IDSLYSEGV LKLGLQD+LLKKLPLP +V+   ESSQ  GLL+Y+TPKILSKDKFAWLR
Sbjct: 361 QIDSLYSEGVFLKLGLQDDLLKKLPLPNLVTRLHESSQGGGLLKYDTPKILSKDKFAWLR 420

Query: 421 DDEFARQAIAGINPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALD 480
           DDEFARQ IAG+NPV+IE+LKVFPPVS LDP+ +GPQESAL+EEHI+G L+G TV+QA++
Sbjct: 421 DDEFARQTIAGVNPVSIEKLKVFPPVSQLDPEKHGPQESALREEHIVGFLDGRTVKQAIE 480

Query: 481 ENKLFIVDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLPPTGPSSR 540
           E+KLFI+DYHD+YLPF+DRINALDGRK YATRTIF+L P GTLKP+AIELSLP   P S 
Sbjct: 481 EDKLFIIDYHDIYLPFLDRINALDGRKAYATRTIFYLNPSGTLKPVAIELSLPQALPGSE 540

Query: 541 SKRIVTPPVDATSNWIWQLAKAHVCSNDAGVHQLVNHW 572
           SKR++TPP DATSNW+WQLAKAH CSNDAG HQLV+H+
Sbjct: 541 SKRVLTPPSDATSNWMWQLAKAHXCSNDAGAHQLVHHF 568

BLAST of Lcy05g000810 vs. ExPASy Swiss-Prot
Match: Q8H016 (Probable lipoxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0179900 PE=2 SV=2)

HSP 1 Score: 711.4 bits (1835), Expect = 7.9e-204
Identity = 356/557 (63.91%), Postives = 425/557 (76.30%), Query Frame = 0

Query: 31  EKQFRPIWAVPVEKRKVVHLRKAVNGPVAAISEDL----------VKAVPLAEK---PVK 90
           E +F P+  +P E+    H R+ V   VAAISE++          +K     E+   P K
Sbjct: 26  ELRFAPL--LPGER----HGRRKV--VVAAISEEVPRLAASPSSGIKGGGAGERRPAPEK 85

Query: 91  FKVRAVVTIRNKNKEDIKETIVKHLDAFTDRIGQNVVLQLISTEIDPKTNAPKKSNEAVL 150
             +RA +T+R K KEDIKE +  HLDA  D +G+NVVL+LIST+I P+T  P +S    +
Sbjct: 86  VALRAALTVRRKQKEDIKEAVAGHLDALWDMVGRNVVLELISTKIHPRTKKPMQSGRVSI 145

Query: 151 KDWSKKTNLKAERVNYTAEFLLTSDFGELGAITITNKHQQEFFLETITIE--QFASDPIH 210
           KDW +K   K + V YTAEF + +DFGE GAI + N+H +EFFLE+I +E       P+H
Sbjct: 146 KDWCQKRGAKGDHVVYTAEFTVDADFGEPGAIAVANRHNREFFLESIVVEGGGLPCGPVH 205

Query: 211 FPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIKALREKELKDIRGDGRGERKLSDRVY 270
           F CNSWVQS ++ P KR+FFSNKPYLP ETP G++ LREKELKD+RGDG G RKLSDR+Y
Sbjct: 206 FACNSWVQSTRELPTKRVFFSNKPYLPSETPPGLRELREKELKDLRGDGTGVRKLSDRIY 265

Query: 271 DFDVYNDLGNPDKGIEFARPRLGGEKVPYPRRCRTGRPPSDTDITAESRVEKPLPMYVPR 330
           D+  YNDLGNPDKG EF RP LGGEK+PYPRRCRTGRPP+DT++ AESRVEKP P+YVPR
Sbjct: 266 DYATYNDLGNPDKGKEFIRPILGGEKIPYPRRCRTGRPPTDTNMLAESRVEKPHPIYVPR 325

Query: 331 DEQFEESKQTTFSLGRLKAVLHNLIPSLKASILSNKHDFHGFSDIDSLYSEGVLLKLGLQ 390
           DE FEE KQ  FS GRL+AVLH LIPSL ASI +  H+F GF  ID+LY EG+ LKLGLQ
Sbjct: 326 DEAFEELKQGAFSSGRLRAVLHTLIPSLIASISAETHNFQGFHHIDNLYKEGLRLKLGLQ 385

Query: 391 DELLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFARQAIAGINPVNIERLKV 450
           + L +K+PL + + ESS+G+LRY+TP ILSKDKFAWLRDDEFARQA+AGINPVNIERL+V
Sbjct: 386 EHLFQKIPLVQKIQESSEGMLRYDTPSILSKDKFAWLRDDEFARQAVAGINPVNIERLQV 445

Query: 451 FPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDENKLFIVDYHDVYLPFIDRINA 510
           FPPVS LDP +YGP ES++ E HI G LNG+TVQQA+DE KLFIVDYHD YLPF+DRINA
Sbjct: 446 FPPVSKLDPAIYGPPESSITETHIAGHLNGLTVQQAMDEAKLFIVDYHDAYLPFLDRINA 505

Query: 511 LDGRKTYATRTIFFLTPLGTLKPIAIELSLPPTGPSS-RSKRIVTPPVDATSNWIWQLAK 570
           +DGRK YATRTIFFLT  GTLKPIAIELSLPP  P   R  +++TPP DATSNW+W LAK
Sbjct: 506 IDGRKAYATRTIFFLTEAGTLKPIAIELSLPPAKPGEPRPSKVLTPPYDATSNWLWMLAK 565

Query: 571 AHVCSNDAGVHQLVNHW 572
           AHV SNDAGVHQLVNHW
Sbjct: 566 AHVSSNDAGVHQLVNHW 574

BLAST of Lcy05g000810 vs. ExPASy TrEMBL
Match: A0A6J1DEN6 (Lipoxygenase OS=Momordica charantia OX=3673 GN=LOC111019373 PE=3 SV=1)

HSP 1 Score: 1082.8 bits (2799), Expect = 0.0e+00
Identity = 537/571 (94.05%), Postives = 554/571 (97.02%), Query Frame = 0

Query: 1   MALANEIIGSTFLHKSSLASQSKLFQTGSFEKQFRPIWAVPVEKRKVVHLRKAVNGPVAA 60
           MALANE+IGS+FL KSSL S SKLFQ+GSFEKQFRPIW VP EK KVVHLRKAV+GPVAA
Sbjct: 1   MALANEMIGSSFLQKSSLVSSSKLFQSGSFEKQFRPIW-VPGEKMKVVHLRKAVSGPVAA 60

Query: 61  ISEDLVKAVPLAEKPVKFKVRAVVTIRNKNKEDIKETIVKHLDAFTDRIGQNVVLQLIST 120
           ISEDL+KA+PLAEKPVKFKVRAVVTIRNKNKED+KETI KHLDAFTD+IGQNVVLQLIST
Sbjct: 61  ISEDLIKALPLAEKPVKFKVRAVVTIRNKNKEDLKETIFKHLDAFTDKIGQNVVLQLIST 120

Query: 121 EIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNYTAEFLLTSDFGELGAITITNKHQQEFF 180
           EIDPKTNAPKKSNEAVLKDWSKKTN+KAERVNYTAEFLLTSDFGE GAITI NKHQQEFF
Sbjct: 121 EIDPKTNAPKKSNEAVLKDWSKKTNVKAERVNYTAEFLLTSDFGEPGAITIVNKHQQEFF 180

Query: 181 LETITIEQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIKALREKELKDI 240
           LETIT+EQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIKALREKELKDI
Sbjct: 181 LETITLEQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIKALREKELKDI 240

Query: 241 RGDGRGERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEKVPYPRRCRTGRPPSDTDIT 300
           RGDG+ ERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEK+PYPRRCRTGR P+DTDIT
Sbjct: 241 RGDGKEERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEKIPYPRRCRTGRAPTDTDIT 300

Query: 301 AESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASILSNKHDFHGFSDI 360
           AESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASI SNK DFHGFSDI
Sbjct: 301 AESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASIRSNKQDFHGFSDI 360

Query: 361 DSLYSEGVLLKLGLQDELLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFARQ 420
           DSLYSEGVLLKLGLQDELLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEF RQ
Sbjct: 361 DSLYSEGVLLKLGLQDELLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFGRQ 420

Query: 421 AIAGINPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDENKLFIV 480
           AIAG+NPVNIERLKVFPPVSNLDPDVYGPQES+LKEEHILGQLNGMTVQQALDE KLFIV
Sbjct: 421 AIAGVNPVNIERLKVFPPVSNLDPDVYGPQESSLKEEHILGQLNGMTVQQALDEKKLFIV 480

Query: 481 DYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLPPTGPSSRSKRIVTP 540
           DYHDVYLPFIDRINALDGRK YATRTIFFLTPLGTLKPIAIELSLPPTGPSSRSKRIVTP
Sbjct: 481 DYHDVYLPFIDRINALDGRKAYATRTIFFLTPLGTLKPIAIELSLPPTGPSSRSKRIVTP 540

Query: 541 PVDATSNWIWQLAKAHVCSNDAGVHQLVNHW 572
           P DATSNW+WQLAK+HVCSNDAGVHQLVNHW
Sbjct: 541 PADATSNWLWQLAKSHVCSNDAGVHQLVNHW 570

BLAST of Lcy05g000810 vs. ExPASy TrEMBL
Match: A0A6J1GNS2 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111456143 PE=3 SV=1)

HSP 1 Score: 1068.5 bits (2762), Expect = 9.3e-309
Identity = 532/572 (93.01%), Postives = 552/572 (96.50%), Query Frame = 0

Query: 1   MALANEIIGSTFLHKSSLASQSKLFQTGSFEKQFRPIWAVPVEKRKVV-HLRKAVNGPVA 60
           MALANE +GS+FLHK+SL SQSKLFQ G  EKQFRP   VP+EK+++V +LRKAVNGPVA
Sbjct: 1   MALANETMGSSFLHKTSLVSQSKLFQRGYLEKQFRPFGVVPMEKKRIVMNLRKAVNGPVA 60

Query: 61  AISEDLVKAVPLAEKPVKFKVRAVVTIRNKNKEDIKETIVKHLDAFTDRIGQNVVLQLIS 120
           AISEDLVKAVPLAEKPVKFKVRAVVTIRNKNKEDIKETIVK+LDAFTDRIGQNVVLQLIS
Sbjct: 61  AISEDLVKAVPLAEKPVKFKVRAVVTIRNKNKEDIKETIVKNLDAFTDRIGQNVVLQLIS 120

Query: 121 TEIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNYTAEFLLTSDFGELGAITITNKHQQEF 180
           TEIDPKTN PKKSNEAVLKDWSKKTNLK E+VNY AEFLLTSDFGE GAITITNKHQQEF
Sbjct: 121 TEIDPKTNTPKKSNEAVLKDWSKKTNLKVEKVNYIAEFLLTSDFGEPGAITITNKHQQEF 180

Query: 181 FLETITIEQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIKALREKELKD 240
           FLETITIEQ ASDPIHFPCNSWVQSRKDHPAKRIFFSNK YLPGETPAGIKALRE+ELKD
Sbjct: 181 FLETITIEQLASDPIHFPCNSWVQSRKDHPAKRIFFSNKSYLPGETPAGIKALREQELKD 240

Query: 241 IRGDGRGERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEKVPYPRRCRTGRPPSDTDI 300
           IRGDG+GERKLSDRVYDFDVYNDLGNPDKGIEF+RPRLGGEK+PYPRRCRTGR PSDTDI
Sbjct: 241 IRGDGKGERKLSDRVYDFDVYNDLGNPDKGIEFSRPRLGGEKIPYPRRCRTGRAPSDTDI 300

Query: 301 TAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASILSNKHDFHGFSD 360
           TAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVL+NLIPSLKASILSNKHDFHGFSD
Sbjct: 301 TAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLYNLIPSLKASILSNKHDFHGFSD 360

Query: 361 IDSLYSEGVLLKLGLQDELLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFAR 420
           IDSLYS+GVLLKLGLQD+LLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEF R
Sbjct: 361 IDSLYSKGVLLKLGLQDKLLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFGR 420

Query: 421 QAIAGINPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDENKLFI 480
           QAIAG+NPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDE KLFI
Sbjct: 421 QAIAGVNPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDEKKLFI 480

Query: 481 VDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLPPTGPSSRSKRIVT 540
           VDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLP T PSSRSKR+VT
Sbjct: 481 VDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLPSTAPSSRSKRVVT 540

Query: 541 PPVDATSNWIWQLAKAHVCSNDAGVHQLVNHW 572
           PPV+ATSNWIWQLAKAHVCSNDAGVHQLVNHW
Sbjct: 541 PPVEATSNWIWQLAKAHVCSNDAGVHQLVNHW 572

BLAST of Lcy05g000810 vs. ExPASy TrEMBL
Match: A0A6J1JY38 (Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111488540 PE=3 SV=1)

HSP 1 Score: 1063.9 bits (2750), Expect = 2.3e-307
Identity = 528/572 (92.31%), Postives = 552/572 (96.50%), Query Frame = 0

Query: 1   MALANEIIGSTFLHKSSLASQSKLFQTGSFEKQFRPIWAVPVEKRKVV-HLRKAVNGPVA 60
           MA+ANE +GS+FLHK+SL S+SKLFQ G  EKQFRP   VP+EK+++V +LRKAVNGPVA
Sbjct: 1   MAVANETMGSSFLHKTSLVSKSKLFQRGYLEKQFRPFRVVPMEKKRIVMNLRKAVNGPVA 60

Query: 61  AISEDLVKAVPLAEKPVKFKVRAVVTIRNKNKEDIKETIVKHLDAFTDRIGQNVVLQLIS 120
           AISEDLVKAVPLAEKPVKFKVRAVVTIRNKNKEDIKETIVK+LDAFTDRIGQNVVLQLIS
Sbjct: 61  AISEDLVKAVPLAEKPVKFKVRAVVTIRNKNKEDIKETIVKNLDAFTDRIGQNVVLQLIS 120

Query: 121 TEIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNYTAEFLLTSDFGELGAITITNKHQQEF 180
           TEIDPKTN PKKSNEAVLKDWSKKTNLK E+VNYTAEFLLTSDFG  GAITITNKHQQEF
Sbjct: 121 TEIDPKTNTPKKSNEAVLKDWSKKTNLKVEKVNYTAEFLLTSDFGAPGAITITNKHQQEF 180

Query: 181 FLETITIEQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIKALREKELKD 240
           FLETITIEQ ASDPIHFPCNSWVQSRKDHPAKRIFFSNK YLPGETP GIKALRE+ELK+
Sbjct: 181 FLETITIEQLASDPIHFPCNSWVQSRKDHPAKRIFFSNKSYLPGETPEGIKALREQELKE 240

Query: 241 IRGDGRGERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEKVPYPRRCRTGRPPSDTDI 300
           IRGDG+GERKLSDRVYDFDVYNDLGNPDKGIEF+RPRLGGEK+PYPRRCRTGR PSDTDI
Sbjct: 241 IRGDGKGERKLSDRVYDFDVYNDLGNPDKGIEFSRPRLGGEKIPYPRRCRTGRAPSDTDI 300

Query: 301 TAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASILSNKHDFHGFSD 360
           TAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVL+NLIPSLKASILSNKHDFHGFSD
Sbjct: 301 TAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLYNLIPSLKASILSNKHDFHGFSD 360

Query: 361 IDSLYSEGVLLKLGLQDELLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFAR 420
           IDSLYS+GVLLKLGLQD+LLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEF R
Sbjct: 361 IDSLYSKGVLLKLGLQDKLLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFGR 420

Query: 421 QAIAGINPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDENKLFI 480
           QAIAG+NPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDENKLF+
Sbjct: 421 QAIAGVNPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDENKLFV 480

Query: 481 VDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLPPTGPSSRSKRIVT 540
           VDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLP T PSSRSKR+VT
Sbjct: 481 VDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLPSTAPSSRSKRVVT 540

Query: 541 PPVDATSNWIWQLAKAHVCSNDAGVHQLVNHW 572
           PPV+ATSNWIWQLAKAHVCSNDAGVHQLVNHW
Sbjct: 541 PPVEATSNWIWQLAKAHVCSNDAGVHQLVNHW 572

BLAST of Lcy05g000810 vs. ExPASy TrEMBL
Match: A0A5D3BW03 (Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001300 PE=3 SV=1)

HSP 1 Score: 1054.3 bits (2725), Expect = 1.8e-304
Identity = 531/572 (92.83%), Postives = 544/572 (95.10%), Query Frame = 0

Query: 1   MALANEIIGSTFLHKSSLASQSKLFQTGSFEKQFRPIWAVPVEKRKVV-HLRKAVNGPVA 60
           MALANEIIGS+FLHK+S  S S  FQ   F   FRPIW VPV KR+VV  LRKAVN PVA
Sbjct: 1   MALANEIIGSSFLHKTS--SVSSQFQGKQF---FRPIWVVPVAKRQVVAQLRKAVNSPVA 60

Query: 61  AISEDLVKAVPLAEKPVKFKVRAVVTIRNKNKEDIKETIVKHLDAFTDRIGQNVVLQLIS 120
           AISEDLVK VPL EKPVK+KVRAVVTIRNKNKEDIKETIVKHLDA TDRIGQNVVLQLIS
Sbjct: 61  AISEDLVKVVPLDEKPVKYKVRAVVTIRNKNKEDIKETIVKHLDALTDRIGQNVVLQLIS 120

Query: 121 TEIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNYTAEFLLTSDFGELGAITITNKHQQEF 180
           TEIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNY AEFLLTSDFGE GAITITNKHQQEF
Sbjct: 121 TEIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNYIAEFLLTSDFGEPGAITITNKHQQEF 180

Query: 181 FLETITIEQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIKALREKELKD 240
           FLETITIEQFA+DPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIK LRE ELKD
Sbjct: 181 FLETITIEQFANDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIKKLREIELKD 240

Query: 241 IRGDGRGERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEKVPYPRRCRTGRPPSDTDI 300
           IRGDG+GERKLSDRVYDFDVYNDLGNPDKGIE+ARPRLGGEK+PYPRRCRTGR PSDTD+
Sbjct: 241 IRGDGKGERKLSDRVYDFDVYNDLGNPDKGIEYARPRLGGEKIPYPRRCRTGRAPSDTDM 300

Query: 301 TAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASILSNKHDFHGFSD 360
           TAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASILSNKHDFHGFSD
Sbjct: 301 TAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASILSNKHDFHGFSD 360

Query: 361 IDSLYSEGVLLKLGLQDELLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFAR 420
           IDSLYSEGVLLKLGLQDELLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFAR
Sbjct: 361 IDSLYSEGVLLKLGLQDELLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFAR 420

Query: 421 QAIAGINPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDENKLFI 480
           QAIAG+NPVNIERLKVFPPVSNLDPDVYGP ES+LKEEHILGQ+NGMTVQQALDENKLFI
Sbjct: 421 QAIAGVNPVNIERLKVFPPVSNLDPDVYGPLESSLKEEHILGQINGMTVQQALDENKLFI 480

Query: 481 VDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLPPTGPSSRSKRIVT 540
           VDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLP T PSSRSKR+VT
Sbjct: 481 VDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLPSTAPSSRSKRVVT 540

Query: 541 PPVDATSNWIWQLAKAHVCSNDAGVHQLVNHW 572
           PPVDATSNW WQLAKAHVCSNDAGVHQLVNHW
Sbjct: 541 PPVDATSNWTWQLAKAHVCSNDAGVHQLVNHW 567

BLAST of Lcy05g000810 vs. ExPASy TrEMBL
Match: A0A1S3CGK4 (Lipoxygenase OS=Cucumis melo OX=3656 GN=LOC103500686 PE=3 SV=1)

HSP 1 Score: 1054.3 bits (2725), Expect = 1.8e-304
Identity = 531/572 (92.83%), Postives = 544/572 (95.10%), Query Frame = 0

Query: 1   MALANEIIGSTFLHKSSLASQSKLFQTGSFEKQFRPIWAVPVEKRKVV-HLRKAVNGPVA 60
           MALANEIIGS+FLHK+S  S S  FQ   F   FRPIW VPV KR+VV  LRKAVN PVA
Sbjct: 1   MALANEIIGSSFLHKTS--SVSSQFQGKQF---FRPIWVVPVAKRQVVAQLRKAVNSPVA 60

Query: 61  AISEDLVKAVPLAEKPVKFKVRAVVTIRNKNKEDIKETIVKHLDAFTDRIGQNVVLQLIS 120
           AISEDLVK VPL EKPVK+KVRAVVTIRNKNKEDIKETIVKHLDA TDRIGQNVVLQLIS
Sbjct: 61  AISEDLVKVVPLDEKPVKYKVRAVVTIRNKNKEDIKETIVKHLDALTDRIGQNVVLQLIS 120

Query: 121 TEIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNYTAEFLLTSDFGELGAITITNKHQQEF 180
           TEIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNY AEFLLTSDFGE GAITITNKHQQEF
Sbjct: 121 TEIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNYIAEFLLTSDFGEPGAITITNKHQQEF 180

Query: 181 FLETITIEQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIKALREKELKD 240
           FLETITIEQFA+DPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIK LRE ELKD
Sbjct: 181 FLETITIEQFANDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIKKLREIELKD 240

Query: 241 IRGDGRGERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEKVPYPRRCRTGRPPSDTDI 300
           IRGDG+GERKLSDRVYDFDVYNDLGNPDKGIE+ARPRLGGEK+PYPRRCRTGR PSDTD+
Sbjct: 241 IRGDGKGERKLSDRVYDFDVYNDLGNPDKGIEYARPRLGGEKIPYPRRCRTGRAPSDTDM 300

Query: 301 TAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASILSNKHDFHGFSD 360
           TAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASILSNKHDFHGFSD
Sbjct: 301 TAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASILSNKHDFHGFSD 360

Query: 361 IDSLYSEGVLLKLGLQDELLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFAR 420
           IDSLYSEGVLLKLGLQDELLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFAR
Sbjct: 361 IDSLYSEGVLLKLGLQDELLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFAR 420

Query: 421 QAIAGINPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDENKLFI 480
           QAIAG+NPVNIERLKVFPPVSNLDPDVYGP ES+LKEEHILGQ+NGMTVQQALDENKLFI
Sbjct: 421 QAIAGVNPVNIERLKVFPPVSNLDPDVYGPLESSLKEEHILGQINGMTVQQALDENKLFI 480

Query: 481 VDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLPPTGPSSRSKRIVT 540
           VDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLP T PSSRSKR+VT
Sbjct: 481 VDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLPSTAPSSRSKRVVT 540

Query: 541 PPVDATSNWIWQLAKAHVCSNDAGVHQLVNHW 572
           PPVDATSNW WQLAKAHVCSNDAGVHQLVNHW
Sbjct: 541 PPVDATSNWTWQLAKAHVCSNDAGVHQLVNHW 567

BLAST of Lcy05g000810 vs. NCBI nr
Match: XP_022151441.1 (linoleate 13S-lipoxygenase 3-1, chloroplastic-like, partial [Momordica charantia])

HSP 1 Score: 1082.8 bits (2799), Expect = 0.0e+00
Identity = 537/571 (94.05%), Postives = 554/571 (97.02%), Query Frame = 0

Query: 1   MALANEIIGSTFLHKSSLASQSKLFQTGSFEKQFRPIWAVPVEKRKVVHLRKAVNGPVAA 60
           MALANE+IGS+FL KSSL S SKLFQ+GSFEKQFRPIW VP EK KVVHLRKAV+GPVAA
Sbjct: 1   MALANEMIGSSFLQKSSLVSSSKLFQSGSFEKQFRPIW-VPGEKMKVVHLRKAVSGPVAA 60

Query: 61  ISEDLVKAVPLAEKPVKFKVRAVVTIRNKNKEDIKETIVKHLDAFTDRIGQNVVLQLIST 120
           ISEDL+KA+PLAEKPVKFKVRAVVTIRNKNKED+KETI KHLDAFTD+IGQNVVLQLIST
Sbjct: 61  ISEDLIKALPLAEKPVKFKVRAVVTIRNKNKEDLKETIFKHLDAFTDKIGQNVVLQLIST 120

Query: 121 EIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNYTAEFLLTSDFGELGAITITNKHQQEFF 180
           EIDPKTNAPKKSNEAVLKDWSKKTN+KAERVNYTAEFLLTSDFGE GAITI NKHQQEFF
Sbjct: 121 EIDPKTNAPKKSNEAVLKDWSKKTNVKAERVNYTAEFLLTSDFGEPGAITIVNKHQQEFF 180

Query: 181 LETITIEQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIKALREKELKDI 240
           LETIT+EQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIKALREKELKDI
Sbjct: 181 LETITLEQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIKALREKELKDI 240

Query: 241 RGDGRGERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEKVPYPRRCRTGRPPSDTDIT 300
           RGDG+ ERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEK+PYPRRCRTGR P+DTDIT
Sbjct: 241 RGDGKEERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEKIPYPRRCRTGRAPTDTDIT 300

Query: 301 AESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASILSNKHDFHGFSDI 360
           AESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASI SNK DFHGFSDI
Sbjct: 301 AESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASIRSNKQDFHGFSDI 360

Query: 361 DSLYSEGVLLKLGLQDELLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFARQ 420
           DSLYSEGVLLKLGLQDELLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEF RQ
Sbjct: 361 DSLYSEGVLLKLGLQDELLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFGRQ 420

Query: 421 AIAGINPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDENKLFIV 480
           AIAG+NPVNIERLKVFPPVSNLDPDVYGPQES+LKEEHILGQLNGMTVQQALDE KLFIV
Sbjct: 421 AIAGVNPVNIERLKVFPPVSNLDPDVYGPQESSLKEEHILGQLNGMTVQQALDEKKLFIV 480

Query: 481 DYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLPPTGPSSRSKRIVTP 540
           DYHDVYLPFIDRINALDGRK YATRTIFFLTPLGTLKPIAIELSLPPTGPSSRSKRIVTP
Sbjct: 481 DYHDVYLPFIDRINALDGRKAYATRTIFFLTPLGTLKPIAIELSLPPTGPSSRSKRIVTP 540

Query: 541 PVDATSNWIWQLAKAHVCSNDAGVHQLVNHW 572
           P DATSNW+WQLAK+HVCSNDAGVHQLVNHW
Sbjct: 541 PADATSNWLWQLAKSHVCSNDAGVHQLVNHW 570

BLAST of Lcy05g000810 vs. NCBI nr
Match: XP_022953691.1 (linoleate 13S-lipoxygenase 3-1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1068.5 bits (2762), Expect = 1.9e-308
Identity = 532/572 (93.01%), Postives = 552/572 (96.50%), Query Frame = 0

Query: 1   MALANEIIGSTFLHKSSLASQSKLFQTGSFEKQFRPIWAVPVEKRKVV-HLRKAVNGPVA 60
           MALANE +GS+FLHK+SL SQSKLFQ G  EKQFRP   VP+EK+++V +LRKAVNGPVA
Sbjct: 1   MALANETMGSSFLHKTSLVSQSKLFQRGYLEKQFRPFGVVPMEKKRIVMNLRKAVNGPVA 60

Query: 61  AISEDLVKAVPLAEKPVKFKVRAVVTIRNKNKEDIKETIVKHLDAFTDRIGQNVVLQLIS 120
           AISEDLVKAVPLAEKPVKFKVRAVVTIRNKNKEDIKETIVK+LDAFTDRIGQNVVLQLIS
Sbjct: 61  AISEDLVKAVPLAEKPVKFKVRAVVTIRNKNKEDIKETIVKNLDAFTDRIGQNVVLQLIS 120

Query: 121 TEIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNYTAEFLLTSDFGELGAITITNKHQQEF 180
           TEIDPKTN PKKSNEAVLKDWSKKTNLK E+VNY AEFLLTSDFGE GAITITNKHQQEF
Sbjct: 121 TEIDPKTNTPKKSNEAVLKDWSKKTNLKVEKVNYIAEFLLTSDFGEPGAITITNKHQQEF 180

Query: 181 FLETITIEQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIKALREKELKD 240
           FLETITIEQ ASDPIHFPCNSWVQSRKDHPAKRIFFSNK YLPGETPAGIKALRE+ELKD
Sbjct: 181 FLETITIEQLASDPIHFPCNSWVQSRKDHPAKRIFFSNKSYLPGETPAGIKALREQELKD 240

Query: 241 IRGDGRGERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEKVPYPRRCRTGRPPSDTDI 300
           IRGDG+GERKLSDRVYDFDVYNDLGNPDKGIEF+RPRLGGEK+PYPRRCRTGR PSDTDI
Sbjct: 241 IRGDGKGERKLSDRVYDFDVYNDLGNPDKGIEFSRPRLGGEKIPYPRRCRTGRAPSDTDI 300

Query: 301 TAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASILSNKHDFHGFSD 360
           TAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVL+NLIPSLKASILSNKHDFHGFSD
Sbjct: 301 TAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLYNLIPSLKASILSNKHDFHGFSD 360

Query: 361 IDSLYSEGVLLKLGLQDELLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFAR 420
           IDSLYS+GVLLKLGLQD+LLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEF R
Sbjct: 361 IDSLYSKGVLLKLGLQDKLLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFGR 420

Query: 421 QAIAGINPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDENKLFI 480
           QAIAG+NPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDE KLFI
Sbjct: 421 QAIAGVNPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDEKKLFI 480

Query: 481 VDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLPPTGPSSRSKRIVT 540
           VDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLP T PSSRSKR+VT
Sbjct: 481 VDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLPSTAPSSRSKRVVT 540

Query: 541 PPVDATSNWIWQLAKAHVCSNDAGVHQLVNHW 572
           PPV+ATSNWIWQLAKAHVCSNDAGVHQLVNHW
Sbjct: 541 PPVEATSNWIWQLAKAHVCSNDAGVHQLVNHW 572

BLAST of Lcy05g000810 vs. NCBI nr
Match: KAG6575441.1 (Linoleate 13S-lipoxygenase 3-1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013983.1 Linoleate 13S-lipoxygenase 3-1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1067.4 bits (2759), Expect = 4.3e-308
Identity = 531/572 (92.83%), Postives = 552/572 (96.50%), Query Frame = 0

Query: 1   MALANEIIGSTFLHKSSLASQSKLFQTGSFEKQFRPIWAVPVEKRKVV-HLRKAVNGPVA 60
           MALANE +GS+FLHK+SL SQS+LFQ G  EKQFRP   VP+EK+++V +LRKAVNGPVA
Sbjct: 1   MALANETMGSSFLHKTSLVSQSRLFQRGYLEKQFRPFGVVPMEKKRIVMNLRKAVNGPVA 60

Query: 61  AISEDLVKAVPLAEKPVKFKVRAVVTIRNKNKEDIKETIVKHLDAFTDRIGQNVVLQLIS 120
           AISEDLVKAVPLAEKPVKFKVRAVVTIRNKNKEDIKETIVK+LDAFTDRIGQNVVLQLIS
Sbjct: 61  AISEDLVKAVPLAEKPVKFKVRAVVTIRNKNKEDIKETIVKNLDAFTDRIGQNVVLQLIS 120

Query: 121 TEIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNYTAEFLLTSDFGELGAITITNKHQQEF 180
           TEIDPKTN PKKSNEAVLKDWSKKTNLK E+VNY AEFLLTSDFGE GAITITNKHQQEF
Sbjct: 121 TEIDPKTNTPKKSNEAVLKDWSKKTNLKVEKVNYIAEFLLTSDFGEPGAITITNKHQQEF 180

Query: 181 FLETITIEQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIKALREKELKD 240
           FLETITIEQ ASDPIHFPCNSWVQSRKDHPAKRIFFSNK YLPGETPAGIKALRE+ELKD
Sbjct: 181 FLETITIEQLASDPIHFPCNSWVQSRKDHPAKRIFFSNKSYLPGETPAGIKALREQELKD 240

Query: 241 IRGDGRGERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEKVPYPRRCRTGRPPSDTDI 300
           IRGDG+GERKLSDRVYDFDVYNDLGNPDKGIEF+RPRLGGEK+PYPRRCRTGR PSDTDI
Sbjct: 241 IRGDGKGERKLSDRVYDFDVYNDLGNPDKGIEFSRPRLGGEKIPYPRRCRTGRAPSDTDI 300

Query: 301 TAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASILSNKHDFHGFSD 360
           TAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVL+NLIPSLKASILSNKHDFHGFSD
Sbjct: 301 TAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLYNLIPSLKASILSNKHDFHGFSD 360

Query: 361 IDSLYSEGVLLKLGLQDELLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFAR 420
           IDSLYS+GVLLKLGLQD+LLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEF R
Sbjct: 361 IDSLYSKGVLLKLGLQDKLLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFGR 420

Query: 421 QAIAGINPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDENKLFI 480
           QAIAG+NPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDE KLFI
Sbjct: 421 QAIAGVNPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDEKKLFI 480

Query: 481 VDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLPPTGPSSRSKRIVT 540
           VDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLP T PSSRSKR+VT
Sbjct: 481 VDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLPSTAPSSRSKRVVT 540

Query: 541 PPVDATSNWIWQLAKAHVCSNDAGVHQLVNHW 572
           PPV+ATSNWIWQLAKAHVCSNDAGVHQLVNHW
Sbjct: 541 PPVEATSNWIWQLAKAHVCSNDAGVHQLVNHW 572

BLAST of Lcy05g000810 vs. NCBI nr
Match: XP_038898250.1 (linoleate 13S-lipoxygenase 3-1, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 1065.8 bits (2755), Expect = 1.3e-307
Identity = 538/573 (93.89%), Postives = 548/573 (95.64%), Query Frame = 0

Query: 1   MALANEIIGSTFLHKSSLASQSKLFQTGSFEKQ-FRPIWAVPVEKRKVV-HLRKAVNGPV 60
           MALANEI+ S+FLHK+SL SQ   FQ    +KQ FRPIW VPVEKRKVV  LRKAVN PV
Sbjct: 1   MALANEIMTSSFLHKTSLVSQ---FQNDGLDKQFFRPIWVVPVEKRKVVAQLRKAVNSPV 60

Query: 61  AAISEDLVKAVPLAEKPVKFKVRAVVTIRNKNKEDIKETIVKHLDAFTDRIGQNVVLQLI 120
           AAISEDLVK VPLAEKPVK KVRAVVTIRNKNKEDIKETIVKHLDAFTDRIGQNVVLQLI
Sbjct: 61  AAISEDLVKPVPLAEKPVKCKVRAVVTIRNKNKEDIKETIVKHLDAFTDRIGQNVVLQLI 120

Query: 121 STEIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNYTAEFLLTSDFGELGAITITNKHQQE 180
           STEIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNY AEFLLTSDFGE GAITITNKHQQE
Sbjct: 121 STEIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNYIAEFLLTSDFGEPGAITITNKHQQE 180

Query: 181 FFLETITIEQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIKALREKELK 240
           FFLETITIEQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLP ETPAGIK LRE ELK
Sbjct: 181 FFLETITIEQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPDETPAGIKKLREHELK 240

Query: 241 DIRGDGRGERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEKVPYPRRCRTGRPPSDTD 300
           DIRGDG+GERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEK+PYPRRCRTGR PSDTD
Sbjct: 241 DIRGDGKGERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEKIPYPRRCRTGRAPSDTD 300

Query: 301 ITAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASILSNKHDFHGFS 360
           ITAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASILSNK+DFHGFS
Sbjct: 301 ITAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASILSNKNDFHGFS 360

Query: 361 DIDSLYSEGVLLKLGLQDELLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFA 420
           DIDSLYSEGVLLKLGLQDELLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFA
Sbjct: 361 DIDSLYSEGVLLKLGLQDELLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFA 420

Query: 421 RQAIAGINPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDENKLF 480
           RQAIAG+NPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQ+NGMTVQQALDENKLF
Sbjct: 421 RQAIAGVNPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQINGMTVQQALDENKLF 480

Query: 481 IVDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLPPTGPSSRSKRIV 540
           IVDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLG LKPIAIELSLP T PSSRSKR+V
Sbjct: 481 IVDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGALKPIAIELSLPSTAPSSRSKRVV 540

Query: 541 TPPVDATSNWIWQLAKAHVCSNDAGVHQLVNHW 572
           TPPVDATSNWIWQLAKAHVCSNDAGVHQLVNHW
Sbjct: 541 TPPVDATSNWIWQLAKAHVCSNDAGVHQLVNHW 570

BLAST of Lcy05g000810 vs. NCBI nr
Match: XP_023548188.1 (linoleate 13S-lipoxygenase 3-1, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1065.4 bits (2754), Expect = 1.6e-307
Identity = 530/572 (92.66%), Postives = 552/572 (96.50%), Query Frame = 0

Query: 1   MALANEIIGSTFLHKSSLASQSKLFQTGSFEKQFRPIWAVPVEKRKVV-HLRKAVNGPVA 60
           MA+ANE +GS+FLHK+SL SQSKLFQ G  EKQFRP   VP+EK+++V +LRKAVNGPVA
Sbjct: 1   MAVANETMGSSFLHKTSLVSQSKLFQRGYLEKQFRPFRVVPMEKKRIVMNLRKAVNGPVA 60

Query: 61  AISEDLVKAVPLAEKPVKFKVRAVVTIRNKNKEDIKETIVKHLDAFTDRIGQNVVLQLIS 120
           AIS+DLVKAVPLAEKPVKFKVRAVVTIRNKNKEDIKETIVK+LDAFTDRIGQNVVLQLIS
Sbjct: 61  AISKDLVKAVPLAEKPVKFKVRAVVTIRNKNKEDIKETIVKNLDAFTDRIGQNVVLQLIS 120

Query: 121 TEIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNYTAEFLLTSDFGELGAITITNKHQQEF 180
           TEIDPKTN PKKSNEAVLKDWSKKTNLK E+VNY AEFLLTSDFGE GAITITNKHQQEF
Sbjct: 121 TEIDPKTNTPKKSNEAVLKDWSKKTNLKVEKVNYIAEFLLTSDFGEPGAITITNKHQQEF 180

Query: 181 FLETITIEQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIKALREKELKD 240
           FLETITIEQ ASDPIHFPCNSWVQSRKDHPAKRIFFSNK YLPGETPAGIKALRE+ELKD
Sbjct: 181 FLETITIEQLASDPIHFPCNSWVQSRKDHPAKRIFFSNKSYLPGETPAGIKALREQELKD 240

Query: 241 IRGDGRGERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEKVPYPRRCRTGRPPSDTDI 300
           IRGDG+GERKLSDRVYDFDVYNDLGNPDKGIEF+RPRLGGEK+PYPRRCRTGR PSDTDI
Sbjct: 241 IRGDGKGERKLSDRVYDFDVYNDLGNPDKGIEFSRPRLGGEKIPYPRRCRTGRAPSDTDI 300

Query: 301 TAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASILSNKHDFHGFSD 360
           TAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVL+NLIPSLKASILSNKHDFHGFSD
Sbjct: 301 TAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLYNLIPSLKASILSNKHDFHGFSD 360

Query: 361 IDSLYSEGVLLKLGLQDELLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFAR 420
           IDSLYS+GVLLKLGLQD+LLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEF R
Sbjct: 361 IDSLYSKGVLLKLGLQDKLLKKLPLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFGR 420

Query: 421 QAIAGINPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDENKLFI 480
           QAIAG+NPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDE KLFI
Sbjct: 421 QAIAGVNPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDEKKLFI 480

Query: 481 VDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLPPTGPSSRSKRIVT 540
           VDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLP T PSSRSKR+VT
Sbjct: 481 VDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLPSTAPSSRSKRVVT 540

Query: 541 PPVDATSNWIWQLAKAHVCSNDAGVHQLVNHW 572
           PPV+ATSNWIWQLAKAHVCSNDAGVHQLVNHW
Sbjct: 541 PPVEATSNWIWQLAKAHVCSNDAGVHQLVNHW 572

BLAST of Lcy05g000810 vs. TAIR 10
Match: AT1G17420.1 (lipoxygenase 3 )

HSP 1 Score: 810.8 bits (2093), Expect = 6.8e-235
Identity = 405/588 (68.88%), Postives = 482/588 (81.97%), Query Frame = 0

Query: 1   MALANEIIGSTFL-HKSSLASQSKLFQTGSFEKQFRPIWAVPVEKRKVVHLRKAVNGPVA 60
           MALA E++G   +  +SSL S +  F+  +   QF      P ++R     RK  +G VA
Sbjct: 1   MALAKELMGYPLITERSSLVSSASHFKKRTQSTQFS---INPFDRRP----RKTKSGVVA 60

Query: 61  AISEDLVKAVPLA------------EKPVKFKVRAVVTIRNKNKEDIKETIVKHLDAFTD 120
           AISEDLVK +  +            +  VKFKVRAVVT+RNKNKED+KET+VKHLDAF D
Sbjct: 61  AISEDLVKTLRFSTTTGDRKSEEEEKAAVKFKVRAVVTVRNKNKEDLKETLVKHLDAFAD 120

Query: 121 RIGQNVVLQLISTEIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNYTAEFLLTSDFGELG 180
           +IG+N+VL+LIST++DPKT  PKKSN AVLKDWSKK+  KAERV+YTAEF + + FG  G
Sbjct: 121 KIGRNIVLELISTQLDPKTKLPKKSNAAVLKDWSKKSKTKAERVHYTAEFTVDAAFGSPG 180

Query: 181 AITITNKHQQEFFLETITIEQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPA 240
           AIT+ NKHQ+EFFLE+ITIE FA  P+HFPCNSWVQS+KDHP KRIFF+N+PYLP ETP+
Sbjct: 181 AITVMNKHQKEFFLESITIEGFALGPVHFPCNSWVQSQKDHPDKRIFFTNQPYLPNETPS 240

Query: 241 GIKALREKELKDIRGDGRGERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEKVPYPRR 300
           G++ LREKELK++RGDG G RKLSDR+YDFDVYNDLGNPDK  E +RP+LGG++VPYPRR
Sbjct: 241 GLRVLREKELKNLRGDGSGVRKLSDRIYDFDVYNDLGNPDKSSELSRPKLGGKEVPYPRR 300

Query: 301 CRTGRPPSDTDITAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASI 360
           CRTGR  + +D  AESRVEKPLPMYVPRDEQFEESKQ TF+ GRLKAVLH+LIPSLKASI
Sbjct: 301 CRTGRQSTVSDKDAESRVEKPLPMYVPRDEQFEESKQDTFAAGRLKAVLHHLIPSLKASI 360

Query: 361 LSNKHDFHGFSDIDSLYSEGVLLKLGLQDELLKKLPLPRVV----SESSQGLLRYNTPKI 420
           ++   DF  F +ID LY EG+LLKLG QD++ KK PLP+VV     ES++GLL+Y+TPKI
Sbjct: 361 VA--EDFADFGEIDRLYKEGLLLKLGFQDDIFKKFPLPKVVVDTLQESTKGLLKYDTPKI 420

Query: 421 LSKDKFAWLRDDEFARQAIAGINPVNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQL 480
           LSKDK AWLRDDEFARQAIAGINPVNIER+K FPPVSNLDP +YGPQ SAL ++HI+G L
Sbjct: 421 LSKDKNAWLRDDEFARQAIAGINPVNIERVKTFPPVSNLDPKIYGPQHSALTDDHIIGHL 480

Query: 481 NGMTVQQALDENKLFIVDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIEL 540
           +G +VQQAL+EN+L+++DYHD++LPF+DRINALDGRK YATRTIFFLT LGTLKP+AIEL
Sbjct: 481 DGFSVQQALEENRLYMLDYHDIFLPFLDRINALDGRKAYATRTIFFLTRLGTLKPVAIEL 540

Query: 541 SLPPTGPSSRSKRIVTPPVDATSNWIWQLAKAHVCSNDAGVHQLVNHW 572
           SLPP GP  RSKR++TPPVDATSNW+WQLAKAHV SNDAGVHQLVNHW
Sbjct: 541 SLPPHGPKHRSKRVLTPPVDATSNWMWQLAKAHVSSNDAGVHQLVNHW 579

BLAST of Lcy05g000810 vs. TAIR 10
Match: AT1G72520.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 802.7 bits (2072), Expect = 1.9e-232
Identity = 408/592 (68.92%), Postives = 488/592 (82.43%), Query Frame = 0

Query: 1   MALANEIIGSTFLHKSSLASQSKLFQTGSFEKQF-RPIWAV-PVEKRKVVHLRKAVN--G 60
           MALANEI+GS  + + S +  S      S +K+  R  +++ P + R +    +AVN  G
Sbjct: 1   MALANEIMGSRLIFERSSSLASPFHSRFSIKKKTQRTQFSINPFDPRPM----RAVNSSG 60

Query: 61  PVAAISEDLVKAVPLA------------EKPVKFKVRAVVTIRNKNKEDIKETIVKHLDA 120
            VAAISEDLVK + ++            EK VKFKVRAV T+RNKNKED KET+VKHLDA
Sbjct: 61  VVAAISEDLVKTLRISTVGRKQEKEEEEEKSVKFKVRAVATVRNKNKEDFKETLVKHLDA 120

Query: 121 FTDRIGQNVVLQLISTEIDPKTNAPKKSNEAVLKDWSKKTNLKAERVNYTAEFLLTSDFG 180
           FTD+IG+NVVL+L+ST++DPKTN PKKS  AVLKDWSKK+N KAERV+YTAEF + S FG
Sbjct: 121 FTDKIGRNVVLELMSTQVDPKTNEPKKSKAAVLKDWSKKSNSKAERVHYTAEFTVDSAFG 180

Query: 181 ELGAITITNKHQQEFFLETITIEQFASDPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGE 240
             GAIT+TNKHQ+EFFLE+ITIE FA  P+HFPCNSWVQS+KDHP+KRI F+N+PYLP E
Sbjct: 181 SPGAITVTNKHQKEFFLESITIEGFACGPVHFPCNSWVQSQKDHPSKRILFTNQPYLPSE 240

Query: 241 TPAGIKALREKELKDIRGDGRGERKLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEKVPY 300
           TP+G++ LREKEL+++RG+G+GERKLSDR+YD+DVYND+GNPD   E ARP LGG + PY
Sbjct: 241 TPSGLRTLREKELENLRGNGKGERKLSDRIYDYDVYNDIGNPDISRELARPTLGGREFPY 300

Query: 301 PRRCRTGRPPSDTDITAESRVEKPLPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLK 360
           PRRCRTGR  +DTD+ +E RVEKPLPMYVPRDEQFEESKQ TF+  RLKAVLHNLIPSLK
Sbjct: 301 PRRCRTGRSSTDTDMMSERRVEKPLPMYVPRDEQFEESKQNTFAACRLKAVLHNLIPSLK 360

Query: 361 ASILSNKHDFHGFSDIDSLYSEGVLLKLGLQDELLKKLPLPRVVS---ESSQGLLRYNTP 420
           ASIL+   DF  F +IDSLY EG+LLKLG QD++ KK PLP++V+   +SS+GLLRY+TP
Sbjct: 361 ASILA--EDFANFGEIDSLYKEGLLLKLGFQDDMFKKFPLPKIVTTLQKSSEGLLRYDTP 420

Query: 421 KILSKDKFAWLRDDEFARQAIAGINPVNIERLKVFPPVSNLDPDVYGP-QESALKEEHIL 480
           KI+SKDK+AWLRDDEFARQAIAGINPVNIER+  +PPVSNLDP++YGP   SAL E+HI+
Sbjct: 421 KIVSKDKYAWLRDDEFARQAIAGINPVNIERVTSYPPVSNLDPEIYGPGLHSALTEDHII 480

Query: 481 GQLNGMTVQQALDENKLFIVDYHDVYLPFIDRINALDGRKTYATRTIFFLTPLGTLKPIA 540
           GQL+G+TVQQAL+ N+LF+VDYHD+YLPF+DRINALDGRK YATRTI FLT LGTLKPIA
Sbjct: 481 GQLDGLTVQQALETNRLFMVDYHDIYLPFLDRINALDGRKAYATRTILFLTRLGTLKPIA 540

Query: 541 IELSLPPTGPSS-RSKRIVTPPVDATSNWIWQLAKAHVCSNDAGVHQLVNHW 572
           IELSLP    S+ +SKR+VTPPVDATSNW+WQLAKAHV SNDAGVHQLVNHW
Sbjct: 541 IELSLPSQSSSNQKSKRVVTPPVDATSNWMWQLAKAHVGSNDAGVHQLVNHW 586

BLAST of Lcy05g000810 vs. TAIR 10
Match: AT1G67560.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 468.8 bits (1205), Expect = 6.3e-132
Identity = 253/499 (50.70%), Postives = 334/499 (66.93%), Query Frame = 0

Query: 80  VRAVVTIRNKNKEDIKETIVKHLDAFTDRIGQNVVLQLISTEIDPKTNAPKKSNEAVLKD 139
           V AVV IR K KE + E     L+ F   IGQ +++QL+S EIDP+T   +KS E+ +  
Sbjct: 82  VTAVVKIRKKIKEKLTERFEHQLELFMKAIGQGMLIQLVSEEIDPETGKGRKSLESPVMG 141

Query: 140 WSKKTNLKAERVNYTAEFLLTSDFGELGAITITNKHQQEFFLETITIEQFASDPIHFPCN 199
             K        + +TA+F +  +FG+ GAI +TN    E  L  I IE  ++D I FP N
Sbjct: 142 LPKAVK-DPRYLVFTADFTVPINFGKPGAILVTNLLSTEICLSEIIIED-STDTILFPAN 201

Query: 200 SWVQSRKDHPAKRIFFSNKPYLPGETPAGIKALREKELKDIRGDGRGERKLSDRVYDFDV 259
           +W+ S+ D+P  RI F ++P LP ETP GIK LREK+L  +RGDG+GERK  +R+YD+DV
Sbjct: 202 TWIHSKNDNPQARIIFRSQPCLPSETPDGIKELREKDLVSVRGDGKGERKPHERIYDYDV 261

Query: 260 YNDLGNPDKGIEFARPRLGGEKVPYPRRCRTGRPPSDTDITAESRVEKPLPMYVPRDEQF 319
           YNDLG+P K  E  RP LG  + PYPRRCRTGRP    D   ESR ++    YVPRDE F
Sbjct: 262 YNDLGDPRK-TERVRPVLGVPETPYPRRCRTGRPLVSKDPPCESRGKEKEEFYVPRDEVF 321

Query: 320 EESKQTTFSLGRLKAVLHNLIPSLKASILSNKHDFHGFSDIDSLYSEGVLL-KLGLQDEL 379
           EE K+ TF  GR KA+ HNL+PS+ A++ +    F  FSDID+LY   ++L     +D  
Sbjct: 322 EEIKRDTFRAGRFKALFHNLVPSIAAALSNLDIPFTCFSDIDNLYKSNIVLGHTEPKDTG 381

Query: 380 LKKL--PLPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFARQAIAGINPVNIERLKVF 439
           L          +   ++ LL+Y+TP ++  D+FAWLRD+EF RQA+AG+NPVNIE LK  
Sbjct: 382 LGGFIGGFMNGILNVTETLLKYDTPAVIKWDRFAWLRDNEFGRQALAGVNPVNIELLKEL 441

Query: 440 PPVSNLDPDVYGPQESALKEEHILGQLN--GMTVQQALDENKLFIVDYHDVYLPFIDRIN 499
           P  SNLDP +YGPQES L EE I  ++   G T+++AL+E +LF+VDYHD+ LPF+++IN
Sbjct: 442 PIRSNLDPALYGPQESVLTEEIIAREVEHYGTTIEKALEEKRLFLVDYHDILLPFVEKIN 501

Query: 500 AL--DGRKTYATRTIFFLTPLGTLKPIAIELSLPPTGPSSRSKRIVTPPVDATSNWIWQL 559
           ++  D RKTYA+RTIFF +  G L+P+AIELSLPPT   S +K + T   DAT++WIW+L
Sbjct: 502 SIKEDPRKTYASRTIFFYSKNGALRPLAIELSLPPTA-ESENKFVYTHGHDATTHWIWKL 561

Query: 560 AKAHVCSNDAGVHQLVNHW 572
           AKAHVCSNDAGVHQLVNHW
Sbjct: 562 AKAHVCSNDAGVHQLVNHW 576

BLAST of Lcy05g000810 vs. TAIR 10
Match: AT3G45140.1 (lipoxygenase 2 )

HSP 1 Score: 375.2 bits (962), Expect = 9.5e-104
Identity = 197/505 (39.01%), Postives = 316/505 (62.57%), Query Frame = 0

Query: 73  EKPVK-FKVRAVVTIRNKNKEDIKETIVKHLDAFTDRIGQNVVLQLISTEIDPKTNAPKK 132
           ++P++  KV+  +T + +  E I  T  + LD   D  G++++++LIS + D +      
Sbjct: 67  KEPIQNIKVKGYITAQEEFLEGI--TWSRGLDDIADIRGRSLLVELISAKTDQR------ 126

Query: 133 SNEAVLKDWSKKTNLKAERVNYTAEFLLTSDFGELGAITITNKHQQEFFLETITIEQFAS 192
                ++D++++   +A    Y  EF +  DFG +GAI I N++ ++ FL+ + + +   
Sbjct: 127 ---ITVEDYAQRVWAEAPDEKYECEFEMPEDFGPVGAIKIQNQYHRQLFLKGVEL-KLPG 186

Query: 193 DPIHFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIKALREKELKDIRGDGR---GER 252
             I F C SWV  +   P KRIFFS+K YLP +TP  +K  R++EL+ ++G  R   GE 
Sbjct: 187 GSITFTCESWVAPKSVDPTKRIFFSDKSYLPSQTPEPLKKYRKEELETLQGKNREEVGEF 246

Query: 253 KLSDRVYDFDVYNDLGNPDKGIEFARPRLGGEKVPYPRRCRTGRPPSDTDITAESRVEKP 312
              +R+YD+DVYND+G+PD   E ARP +GG   PYPRRC+TGR P +TD ++E R    
Sbjct: 247 TKFERIYDYDVYNDVGDPDNDPELARPVIGGLTHPYPRRCKTGRKPCETDPSSEQRYGG- 306

Query: 313 LPMYVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASILSNKHDFHGFSDIDSLYSEGV 372
              YVPRDE+F  +K T+F+   + A L ++ P +++ +LS +  F  F  I +L+ EG+
Sbjct: 307 -EFYVPRDEEFSTAKGTSFTGKAVLAALPSIFPQIESVLLSPQEPFPHFKAIQNLFEEGI 366

Query: 373 LLKLGLQDELLKKLP-LPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFARQAIAGINP 432
             +L     LL  LP + + + E+   +L+++ P ++++D+F+WLRDDEFARQ +AG+NP
Sbjct: 367 --QLPKDAGLLPLLPRIIKALGEAQDDILQFDAPVLINRDRFSWLRDDEFARQTLAGLNP 426

Query: 433 VNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNG-MTVQQALDENKLFIVDYHDVY 492
            +I+ ++ +P +S LDP VYG   S +  E +  ++ G MTV +AL   +LF++DYHD+ 
Sbjct: 427 YSIQLVEEWPLISKLDPAVYGDPTSLITWEIVEREVKGNMTVDEALKNKRLFVLDYHDLL 486

Query: 493 LPFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLPPTGPSSRSKRIVTPPVDATS 552
           LP+++++  L+    YA+RT+FFL+   TL+P+AIEL+ PP     + K++ TP  DATS
Sbjct: 487 LPYVNKVRELNNTTLYASRTLFFLSDDSTLRPVAIELTCPPNINKPQWKQVFTPGYDATS 546

Query: 553 NWIWQLAKAHVCSNDAGVHQLVNHW 572
            W+W LAK H  S+DAG HQL++HW
Sbjct: 547 CWLWNLAKTHAISHDAGYHQLISHW 555

BLAST of Lcy05g000810 vs. TAIR 10
Match: AT1G55020.1 (lipoxygenase 1 )

HSP 1 Score: 367.9 bits (943), Expect = 1.5e-101
Identity = 208/505 (41.19%), Postives = 293/505 (58.02%), Query Frame = 0

Query: 79  KVRAVVTIRNKNKEDIKETIVKHLDAFTDRIGQNVVLQLISTEIDPKTNAP--KKSNEAV 138
           KV+  V +  KN  D  +     LD   + +G  + L+L+S+++    N    K    A 
Sbjct: 20  KVKGTVVLMKKNVLDFNDFNASFLDRLHEFLGNKITLRLVSSDVTDSENGSKGKLGKAAH 79

Query: 139 LKDW-SKKTNLKAERVNYTAEFLLTSDFGELGAITITNKHQQEFFLETITIEQF-ASDPI 198
           L+DW +  T+L A    +   F   +DFG  GA  I N H  EF L+++T+E       +
Sbjct: 80  LEDWITTITSLTAGESAFKVTFDYETDFGYPGAFLIRNSHFSEFLLKSLTLEDVPGHGRV 139

Query: 199 HFPCNSWVQSRKDHPAKRIFFSNKPYLPGETPAGIKALREKELKDIRGDGRGERKLSDRV 258
           H+ CNSW+   K +   R+FFSNK YLP ETPA +   RE+EL  +RG G GE K  DRV
Sbjct: 140 HYICNSWIYPAKHYTTDRVFFSNKTYLPHETPATLLKYREEELVSLRGTGEGELKEWDRV 199

Query: 259 YDFDVYNDLGNPDKGIEFARPRLGG-EKVPYPRRCRTGRPPSDTDITAESR--VEKPLPM 318
           YD+  YNDLG P K     RP LGG ++ PYPRR RTGR P+  D   ESR  +   L +
Sbjct: 200 YDYAYYNDLGVPPKN---PRPVLGGTQEYPYPRRGRTGRKPTKEDPQTESRLPITSSLDI 259

Query: 319 YVPRDEQFEESKQTTFSLGRLKAVLHNLIPSLKASILSNKHDFHGFSDIDSLYSEGV-LL 378
           YVPRDE+F   K + F    LKA+   + P+L+A       +F  F D+  +Y EG+ L 
Sbjct: 260 YVPRDERFGHLKMSDFLAYALKAIAQFIQPALEAVFDDTPKEFDSFEDVLKIYEEGIDLP 319

Query: 379 KLGLQDELLKKLP---LPRVVSESSQGLLRYNTPKILSKDKFAWLRDDEFARQAIAGINP 438
              L D ++K +P   L  +     Q  L++  P+++ +DK AW  D+EFAR+ +AG+NP
Sbjct: 320 NQALIDSIVKNIPLEMLKEIFRTDGQKFLKFPVPQVIKEDKTAWRTDEEFAREMLAGLNP 379

Query: 439 VNIERLKVFPPVSNLDPDVYGPQESALKEEHILGQLNGMTVQQALDENKLFIVDYHDVYL 498
           V I+ LK FPP S LD + YG Q S + + HI   L+G+TV++AL++ +LFI+D+HD  +
Sbjct: 380 VVIQLLKEFPPKSKLDSESYGNQNSTITKSHIEHNLDGLTVEEALEKERLFILDHHDTLM 439

Query: 499 PFIDRINALDGRKTYATRTIFFLTPLGTLKPIAIELSLP-PTGPSSRSKRIVTPPVDATS 558
           P++ R+N     KTYA+RT+ FL   GTLKP+ IELSLP P G    +   V  P +   
Sbjct: 440 PYLGRVNTTT-TKTYASRTLLFLKDDGTLKPLVIELSLPHPNGDKFGAVSEVYTPGEGVY 499

Query: 559 NWIWQLAKAHVCSNDAGVHQLVNHW 572
           + +WQLAKA V  ND+G HQL++HW
Sbjct: 500 DSLWQLAKAFVGVNDSGNHQLISHW 520

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O243712.7e-25274.70Linoleate 13S-lipoxygenase 3-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LO... [more]
Q9LNR39.6e-23468.88Lipoxygenase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX3 PE=2 SV=1[more]
Q9FNX82.6e-23168.92Lipoxygenase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX4 PE=2 SV=1[more]
R9WTS63.5e-22869.55Lipoxygenase 1, chloroplastic OS=Tanacetum cinerariifolium OX=118510 GN=LOX1 PE=... [more]
Q8H0167.9e-20463.91Probable lipoxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0179900... [more]
Match NameE-valueIdentityDescription
A0A6J1DEN60.0e+0094.05Lipoxygenase OS=Momordica charantia OX=3673 GN=LOC111019373 PE=3 SV=1[more]
A0A6J1GNS29.3e-30993.01Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111456143 PE=3 SV=1[more]
A0A6J1JY382.3e-30792.31Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111488540 PE=3 SV=1[more]
A0A5D3BW031.8e-30492.83Lipoxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001300 ... [more]
A0A1S3CGK41.8e-30492.83Lipoxygenase OS=Cucumis melo OX=3656 GN=LOC103500686 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_022151441.10.0e+0094.05linoleate 13S-lipoxygenase 3-1, chloroplastic-like, partial [Momordica charantia... [more]
XP_022953691.11.9e-30893.01linoleate 13S-lipoxygenase 3-1, chloroplastic-like [Cucurbita moschata][more]
KAG6575441.14.3e-30892.83Linoleate 13S-lipoxygenase 3-1, chloroplastic, partial [Cucurbita argyrosperma s... [more]
XP_038898250.11.3e-30793.89linoleate 13S-lipoxygenase 3-1, chloroplastic-like [Benincasa hispida][more]
XP_023548188.11.6e-30792.66linoleate 13S-lipoxygenase 3-1, chloroplastic-like isoform X1 [Cucurbita pepo su... [more]
Match NameE-valueIdentityDescription
AT1G17420.16.8e-23568.88lipoxygenase 3 [more]
AT1G72520.11.9e-23268.92PLAT/LH2 domain-containing lipoxygenase family protein [more]
AT1G67560.16.3e-13250.70PLAT/LH2 domain-containing lipoxygenase family protein [more]
AT3G45140.19.5e-10439.01lipoxygenase 2 [more]
AT1G55020.11.5e-10141.19lipoxygenase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (P93075) v1
Date Performed: 2021-12-06
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001246Lipoxygenase, plantPRINTSPR00468PLTLPOXGNASEcoord: 396..412
score: 38.8
coord: 251..267
score: 74.59
coord: 186..204
score: 37.4
coord: 502..526
score: 71.23
coord: 212..233
score: 57.54
coord: 283..302
score: 63.09
coord: 351..372
score: 33.85
coord: 435..454
score: 52.98
IPR001024PLAT/LH2 domainSMARTSM00308LH2_4coord: 78..217
e-value: 1.1E-46
score: 171.1
IPR001024PLAT/LH2 domainPFAMPF01477PLATcoord: 130..215
e-value: 1.9E-13
score: 51.0
IPR001024PLAT/LH2 domainPROSITEPS50095PLATcoord: 94..216
score: 22.359932
IPR013819Lipoxygenase, C-terminalPFAMPF00305Lipoxygenasecoord: 229..571
e-value: 1.2E-141
score: 473.5
IPR013819Lipoxygenase, C-terminalPROSITEPS51393LIPOXYGENASE_3coord: 219..571
score: 130.701172
NoneNo IPR availableGENE3D4.10.375.10coord: 239..340
e-value: 9.4E-36
score: 124.2
NoneNo IPR availableGENE3D3.10.450.60coord: 428..561
e-value: 2.5E-52
score: 178.4
NoneNo IPR availableGENE3D2.60.60.20PLAT/LH2 domaincoord: 81..238
e-value: 1.3E-51
score: 176.5
NoneNo IPR availablePANTHERPTHR11771:SF158LIPOXYGENASEcoord: 48..571
IPR027433Lipoxygenase, domain 3GENE3D4.10.372.10coord: 342..427
e-value: 6.0E-20
score: 73.6
IPR000907LipoxygenasePANTHERPTHR11771LIPOXYGENASEcoord: 48..571
IPR042057Plant lipoxygenase, PLAT/LH2 domainCDDcd01751PLAT_LH2coord: 78..217
e-value: 3.81192E-51
score: 170.18
IPR036226Lipoxigenase, C-terminal domain superfamilySUPERFAMILY48484Lipoxigenasecoord: 221..571
IPR036392PLAT/LH2 domain superfamilySUPERFAMILY49723Lipase/lipooxygenase domain (PLAT/LH2 domain)coord: 78..220

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lcy05g000810.1Lcy05g000810.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0034440 lipid oxidation
biological_process GO:0031408 oxylipin biosynthetic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
molecular_function GO:0005515 protein binding
molecular_function GO:0016491 oxidoreductase activity