Lcy04g008290 (gene) Sponge gourd (P93075) v1

BLAST of Lcy04g008290 vs. ExPASy Swiss-Prot
Match: Q9LVL5 (Probable serine/threonine-protein kinase WNK4 OS=Arabidopsis thaliana OX=3702 GN=WNK4 PE=1 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 2.7e-154
Identity = 310/604 (51.32%), Postives = 399/604 (66.06%), Query Frame = 0

Query: 1   MAQPIGYAETDPSGRYGRFREILGKGAMKTVYKAFDEVLGMEVAWNQVQLKDVFHSPEDL 60
           M Q   Y ETDP+GRYGRF EILG+GAMKTVYKA DE LG+EVAW+QV+LK+V  S  DL
Sbjct: 3   MNQVAEYVETDPTGRYGRFAEILGRGAMKTVYKAIDEKLGIEVAWSQVKLKEVLRSSVDL 62

Query: 61  QRLYSEVHLLKNLNHDSIIRFHASWIDTHRRTFNFITEMFTSGSLREYRQKYRNVDIGAI 120
           QRLYSEVHLL  LNH SIIRF+ SWID H  T NFITE+FTSG+LR+Y+ KY  +DI AI
Sbjct: 63  QRLYSEVHLLSTLNHKSIIRFYTSWIDVHNHTLNFITELFTSGTLRQYKNKYLRIDIRAI 122

Query: 121 KNWARQILRGLVYLHEHDPPVIHRDIKCDNIFINGHLGQVKIGDLGLAAILHDSQHAHSV 180
           K+WARQIL GLVYLHEHDPPVIHRD+KCDNIF+NGHLGQVKIGDLGLA +L D   AHS+
Sbjct: 123 KSWARQILEGLVYLHEHDPPVIHRDLKCDNIFVNGHLGQVKIGDLGLARMLRDCHSAHSI 182

Query: 181 IGTPEFMAPELYDEEYNELVDVYSFGMCLIEMLTLEYPYSECSNPAQIYKKVTSGKLPNA 240
           IGTPEFMAPELY+E YNEL+DVYSFGMC +EM+T E+PYSEC++PAQIYKKV  GKLP A
Sbjct: 183 IGTPEFMAPELYEENYNELIDVYSFGMCFLEMITSEFPYSECNHPAQIYKKVVGGKLPGA 242

Query: 241 FYKIKGLEAQKFVRKCLENVSKRLPARELLLDPFLASND------NASDNEELCLSPTPK 300
           FY++  +EAQ+F+ KCL + SKR+ A+ELL DPFLAS++      + + N +  L+    
Sbjct: 243 FYRVGDIEAQRFIGKCLVSASKRVSAKELLQDPFLASDESWMVYTSGAGNPKPFLNENEM 302

Query: 301 KSV-----ARRTDMAISGSMNPKDNDKVFLKVQIKVKNGKAKNVYFAFDTINDTAIDVAT 360
            ++       RT+M+I+G +  +DN K+ L+VQI   NG A NV+F FD +NDT+IDVA 
Sbjct: 303 DTLKLEDDELRTEMSIAGKLGAEDN-KIDLEVQIAYDNGLANNVFFPFDIMNDTSIDVAK 362

Query: 361 EMVKELEISDWDPLEIAVMIKKEISTLIPDWEEWNIPKNHHQDSFNYDVGNDDDDDDDDE 420
           EMVKELEI DW+P+EIA MI   IS+L+ DW+                   +DD+   D 
Sbjct: 363 EMVKELEIIDWEPVEIAKMIDGAISSLVSDWKY-----------------EEDDETPHDH 422

Query: 421 NNVTPHPFYSCSSHASSSNSLQGFYSLYDNPHHFGGRNGTSTATEWFQEDLLIDEDASSS 480
           +      F+S SSHASSS +    Y                   +W Q+DL   ++  S 
Sbjct: 423 HRHRTDSFHSSSSHASSSQASLSNYM-------------ARGLQDWVQDDL--HDETYSQ 482

Query: 481 CSQNSFNYSDVSFDSNNEDDYESDPHLKGREPHNISTTKQPTRFCPTMKIDTHNLRHKDN 540
            S +S +YS++++ +   D+Y S   +  R  HN+      TRFCP  +  +H    + N
Sbjct: 483 SSSHSGSYSNLNYIA--VDEYSSQSPVMSR-THNM------TRFCP--EESSHLQSGQAN 542

Query: 541 QIMLDREFEIQYRSNNNSHRLTRVKSMVNLRSSETLHRSLVEMLLKKRLFNTVGAMENIG 594
                        S+N +  LTR +S+V+++  + LHRS  E   K+RLF TVG +E +G
Sbjct: 543 AYAASSSTNRSLASDNRT--LTRNRSLVDVQ-RQLLHRSPGEEARKRRLFKTVGDVETVG 559

BLAST of Lcy04g008290 vs. ExPASy Swiss-Prot
Match: Q9SCU5 (Probable serine/threonine-protein kinase WNK5 OS=Arabidopsis thaliana OX=3702 GN=WNK5 PE=1 SV=2)

HSP 1 Score: 544.7 bits (1402), Expect = 1.3e-153
Identity = 297/502 (59.16%), Postives = 360/502 (71.71%), Query Frame = 0

Query: 5   IGYAETDPSGRYGRFREILGKGAMKTVYKAFDEVLGMEVAWNQVQLKDVFHSPEDLQRLY 64
           I Y ETDPSGRYGRFRE+LGKGAMKTVYKAFD+VLGMEVAWNQV+L +VF SPE LQRLY
Sbjct: 13  IAYVETDPSGRYGRFREVLGKGAMKTVYKAFDQVLGMEVAWNQVKLNEVFRSPEPLQRLY 72

Query: 65  SEVHLLKNLNHDSIIRFHASWIDTHRRTFNFITEMFTSGSLREYRQKYRNVDIGAIKNWA 124
           SEVHLLKNLNH+SIIR+  SWID +RRTFNFITE+FTSG+LREYR+KY+ VDI AIK+WA
Sbjct: 73  SEVHLLKNLNHESIIRYCTSWIDVNRRTFNFITELFTSGTLREYRRKYQKVDIRAIKSWA 132

Query: 125 RQILRGLVYLHEHDPPVIHRDIKCDNIFINGHLGQVKIGDLGLAAILHDSQHAHSVIGTP 184
           RQIL GL YLH HDPPVIHRD+KCDNIF+NGHLGQVKIGDLGLAAIL  SQ+AHSVIGTP
Sbjct: 133 RQILNGLAYLHGHDPPVIHRDLKCDNIFVNGHLGQVKIGDLGLAAILRGSQNAHSVIGTP 192

Query: 185 EFMAPELYDEEYNELVDVYSFGMCLIEMLTLEYPYSECSNPAQIYKKVTSGKLPNAFYKI 244
           EFMAPELY+E+YNELVD+YSFGMC++EMLT EYPYSEC+NPAQIYKKVTSGKLP++F+ I
Sbjct: 193 EFMAPELYEEDYNELVDIYSFGMCVLEMLTGEYPYSECTNPAQIYKKVTSGKLPDSFHLI 252

Query: 245 KGLEAQKFVRKCLENVSKRLPARELLLDPFLASND-----------------NASDNEEL 304
           +  EAQ+FV KCLE VS+RLPA+ELL DPFLA+ D                 N + N  +
Sbjct: 253 QHTEAQRFVGKCLETVSRRLPAKELLADPFLAATDERDLAPLFRLPQQLAIQNLAANGTV 312

Query: 305 CLSPTPKKSVARRTDMAISGSMNPKDNDKVFLKVQIKVKNGKAKNVYFAFDTINDTAIDV 364
                      R TDM+I+G MN +D+  +FL+VQI   +G  +N+ F F+ ++DT ++V
Sbjct: 313 VEHLPSTTDPTRTTDMSITGKMNSEDH-TIFLQVQILDGDGHMRNIQFPFNILSDTPLEV 372

Query: 365 ATEMVKELEISDWDPLEIAVMIKKEISTLIPDWEEWNIPKNHHQDSFNYDVGNDDDDDDD 424
           A EMVKELEI+DWDPLEIA MI+ EIS L+P+W   +    H  +SF    G++DD+D+ 
Sbjct: 373 ALEMVKELEITDWDPLEIAAMIENEISLLVPNWRANDSSIRH--ESF----GHEDDEDNG 432

Query: 425 DENNVTPHPFYSCSSHASSSNSLQGFYSLYDNPHHFGGRNGTSTATEWFQEDLLIDEDAS 484
           D    T        S ASSS         +D+P      N  S+       D++ D D  
Sbjct: 433 DTEGRT-----RLFSSASSS---------HDSPVAVRENNDDSS------NDVIPDMDDG 487

Query: 485 SSCSQNSFNYSDVSFDSNNEDD 490
           +  S    N S   +    +DD
Sbjct: 493 NRSSNRLLNSSTYHYSPAIDDD 487

BLAST of Lcy04g008290 vs. ExPASy Swiss-Prot
Match: Q2QYL8 (Probable serine/threonine-protein kinase WNK8 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK8 PE=2 SV=1)

HSP 1 Score: 480.7 bits (1236), Expect = 2.4e-134
Identity = 287/607 (47.28%), Postives = 372/607 (61.29%), Query Frame = 0

Query: 6   GYAETDPSGRYGRFREILGKGAMKTVYKAFDEVLGMEVAWNQVQLKDVFHSPEDLQRLYS 65
           GY ETDP+GRYGR  E+LGKGAMKTVY+ FDE+ G+EVAWNQ  + DV  +P+ L R+Y+
Sbjct: 24  GYVETDPTGRYGRLSEVLGKGAMKTVYRGFDELRGVEVAWNQATISDVLRTPDALHRMYA 83

Query: 66  EVHLLKNLNHDSIIRFHASWIDTHRRTFNFITEMFTSGSLREYRQKYRNVDIGAIKNWAR 125
           EV LL +L HD+II FHASW+   RRTFNFITE+F+SG+LR YR +Y  V   A+  WAR
Sbjct: 84  EVSLLADLRHDAIIAFHASWVHPSRRTFNFITELFSSGTLRSYRLRYPRVSRRAVAAWAR 143

Query: 126 QILRGLVYLHEHDPPVIHRDIKCDNIFINGHLGQVKIGDLGLAAILHDSQHAHSVIGTPE 185
            ILRGL YLH     VIHRD+KCDNIF+NGHLGQVKIGDLGLAA+L     A SVIGTPE
Sbjct: 144 AILRGLAYLHSRG--VIHRDLKCDNIFVNGHLGQVKIGDLGLAAVLRGCTSARSVIGTPE 203

Query: 186 FMAPELYDEEYNELVDVYSFGMCLIEMLTLEYPYSECSNPAQIYKKVTSGKLPNAFYKIK 245
           FMAPE+YDE Y   VDVYSFGMC++EMLT EYPYSEC NPAQIYKKVT+GKLP+AFY + 
Sbjct: 204 FMAPEMYDECYGVGVDVYSFGMCMLEMLTNEYPYSECDNPAQIYKKVTAGKLPDAFYLLT 263

Query: 246 GLEAQKFVRKCLENVSKRLPARELLLDPFLASNDNASDNEELCLSPTP------------ 305
             +A++F+ +CL + + R  A ELLLDPFL+   N  D+  +  +  P            
Sbjct: 264 DADARRFIGRCLVDAAHRPSAEELLLDPFLSPPQNHDDHNTIAHATAPPPPLPLACSNSS 323

Query: 306 ------KKSVARRTDMAISGSMNPKDNDKVFLKVQIKVKNGKAKNVYFAFDTINDTAIDV 365
                 +   A+ T MAI+G +N K++D +FLKVQI    G  +N+YF FD  NDTA++V
Sbjct: 324 EEQEEEEAPAAKTTGMAITGKLN-KEHDTIFLKVQIG-GGGNVRNIYFPFDVANDTAMEV 383

Query: 366 ATEMVKELEISDWDPLEIAVMIKKEISTLIPDWEEWNIPKNHHQDSFNYDVGNDDDDDDD 425
           ATEMVKEL+I+D +P EIA MI++EI  L+P +++       H+ S+        DDDDD
Sbjct: 384 ATEMVKELDIADREPTEIAAMIEQEIVRLVPGYKQ-------HEYSY-------ADDDDD 443

Query: 426 DENNVTPHPFYSCSSH-ASSSNSLQGFYSLYDNPHHFGGRNGTSTATEWFQEDLLIDEDA 485
           D+ N  P+PFY  SS   SS  SL G       P    G  G     +W ++        
Sbjct: 444 DDVNGQPNPFYYLSSSPTSSQGSLCGV-----GPTSSEGFPGPHGKVDWSRD-------- 503

Query: 486 SSSCSQNSFNYSDVSFDSNNEDDYESDPHLKGREPHNISTTKQPTRFCPTMKIDTHNLRH 545
                   + Y   S  S ++DD  S   L       IS  +Q      +          
Sbjct: 504 --------YCYYPPSSVSVSDDDDSSTSSLSA-AVSAISLQQQHCSASSSRLGPASASAS 563

Query: 546 KDNQIMLDREFEIQYRSNNNSHRLTRVKSMVNLRSSETLHRSLVEMLLKKRLFNTVGAME 594
           +D      R  + +        R++R +SMV++R S+ LHR+LVE L K+  FNTVGA+ 
Sbjct: 564 EDGGGHAGRPRQREGEEERRRRRMSRNRSMVDMR-SQLLHRTLVEELNKRLFFNTVGAVH 589

BLAST of Lcy04g008290 vs. ExPASy Swiss-Prot
Match: Q2RBE3 (Probable serine/threonine-protein kinase WNK7 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK7 PE=2 SV=2)

HSP 1 Score: 474.9 bits (1221), Expect = 1.3e-132
Identity = 291/615 (47.32%), Postives = 383/615 (62.28%), Query Frame = 0

Query: 6   GYAETDPSGRYGRFREILGKGAMKTVYKAFDEVLGMEVAWNQVQLKDVFHSPEDLQRLYS 65
           GY ETDP+GRYGR  E+LGKGAMKTVY+ FDE+ G+EVAWNQ  + DV  +P+ L R+Y+
Sbjct: 24  GYVETDPTGRYGRLSEVLGKGAMKTVYRGFDELRGVEVAWNQATISDVLRTPDALHRMYA 83

Query: 66  EVHLLKNLNHDSIIRFHASWIDTHRRTFNFITEMFTSGSLREYRQKYRNVDIGAIKNWAR 125
           EV LL +L HD+II FHASW+   RRTFNFITE+F+SG+LR YR +Y  V   A+  WAR
Sbjct: 84  EVSLLADLRHDAIIAFHASWVHPSRRTFNFITELFSSGTLRSYRLRYPRVSRRAVAAWAR 143

Query: 126 QILRGLVYLHEHDPPVIHRDIKCDNIFINGHLGQVKIGDLGLAAILHDSQHAHSVIGTPE 185
            ILRGL YLH     VIHRD+KCDNIF+NGHLGQVKIGDLGLAA+L     A SVIGTPE
Sbjct: 144 AILRGLAYLHARG--VIHRDLKCDNIFVNGHLGQVKIGDLGLAAVLRGCASARSVIGTPE 203

Query: 186 FMAPELYDEEYNELVDVYSFGMCLIEMLTLEYPYSECSNPAQIYKKVTSGKLPNAFYKIK 245
           FMAPE+YDE Y   VDVYSFGMC++EMLT EYPYSEC NPAQIYKKVT+GKLP+AFY++ 
Sbjct: 204 FMAPEMYDECYGVGVDVYSFGMCMLEMLTNEYPYSECDNPAQIYKKVTAGKLPDAFYRLT 263

Query: 246 GLEAQKFVRKCLENVSKRLPARELLLDPFLASNDNASDNEELCLSPTPKKSV-------- 305
             +A++F+ +CL + + R  A ELLLDPFL+ + N  D+  +  +  P   +        
Sbjct: 264 DADARRFIGRCLVDAAHRPSAEELLLDPFLSPSQNHDDHNIIAHATAPPPPLPLACSNSS 323

Query: 306 -----------ARRTDMAISGSMNPKDNDKVFLKVQIKVKNGKAKNVYFAFDTINDTAID 365
                      A+ TDMAI+G +N K++D +FLKVQI       +N+YF FD  NDTA++
Sbjct: 324 EEQEEAAPAPAAKTTDMAITGKLN-KEHDTIFLKVQIG-GGRNVRNIYFPFDVANDTAME 383

Query: 366 VATEMVKELEISDWDPLEIAVMIKKEISTLIPDWEEWNIPKNHHQDSFNYDVGNDDDDDD 425
           VATEMVKEL+I+D +P EIA MI++EI  L+P +++       H+ S+        DD+D
Sbjct: 384 VATEMVKELDIADREPTEIAAMIEQEIVRLVPGYKQ-------HEYSY-------ADDND 443

Query: 426 DDENNVTPHPFYSCSSH-ASSSNSLQGFYSLYDNPHHFGGRNGTSTATEWFQE------- 485
           DD+ +  P+PFY  SS   SS  SL G  S   +   F G +G     +W ++       
Sbjct: 444 DDDVSGHPNPFYYLSSSPTSSQGSLCGVGS--TSSEGFPGPHG---KVDWSRDYCYYPPS 503

Query: 486 DLLIDEDASSSCSQNSFNYSDVSFDSNNEDDYESDPHLKGREPHNISTTKQPTRFCPTMK 545
            + + +D  SS S  S   S  S     +    S   L    P + S ++          
Sbjct: 504 SVSVSDDDDSSTSSLSAAVSASSLHQQQQHCSASSSRL---GPASASASEDGG------- 563

Query: 546 IDTHNLRHKDNQIMLDREFEIQYRSNNNSHRLTRVKSMVNLRSSETLHRSLVEMLLKKRL 594
              H  R +       RE E + R      R++R +SMV++R S+ LHR+LVE L K+  
Sbjct: 564 -GGHAGRPR------QREGEEERR----RRRMSRNRSMVDMR-SQLLHRTLVEELNKRLF 593

BLAST of Lcy04g008290 vs. ExPASy Swiss-Prot
Match: A2YMV6 (Probable serine/threonine-protein kinase WNK1 OS=Oryza sativa subsp. indica OX=39946 GN=WNK1 PE=2 SV=2)

HSP 1 Score: 437.6 bits (1124), Expect = 2.3e-121
Identity = 222/425 (52.24%), Postives = 281/425 (66.12%), Query Frame = 0

Query: 7   YAETDPSGRYGRFREILGKGAMKTVYKAFDEVLGMEVAWNQVQLKDVFHSPEDLQRLYSE 66
           YAE DP+GRYGR+ ++LGKGA KTVY+AFDE  GMEVAWNQV+L D   SPEDL+RLY E
Sbjct: 17  YAEVDPTGRYGRYNDVLGKGASKTVYRAFDEYQGMEVAWNQVKLHDFLQSPEDLERLYCE 76

Query: 67  VHLLKNLNHDSIIRFHASWIDTHRRTFNFITEMFTSGSLREYRQKYRNVDIGAIKNWARQ 126
           +HLLK L H +I++F+ SW+D  RR  NFITEMFTSG+LR+YRQK+  V+I A+K+W RQ
Sbjct: 77  IHLLKTLKHRNIMKFYTSWVDVSRRNINFITEMFTSGTLRQYRQKHMRVNIWAVKHWCRQ 136

Query: 127 ILRGLVYLHEHDPPVIHRDIKCDNIFINGHLGQVKIGDLGLAAILHDSQHAHSVIGTPEF 186
           IL GL+YLH HDPP+IHRD+KCDNIF+NG+ G+VKIGDLGLAAIL  S HA   +GTPEF
Sbjct: 137 ILSGLLYLHSHDPPIIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKS-HAVHCVGTPEF 196

Query: 187 MAPELYDEEYNELVDVYSFGMCLIEMLTLEYPYSECSNPAQIYKKVTSGKLPNAFYKIKG 246
           MAPE+Y+EEYNELVD+YSFGMC++EM+T EYPYSEC++P QIYKKV SG  P A YK+K 
Sbjct: 197 MAPEVYEEEYNELVDIYSFGMCVLEMVTFEYPYSECTHPVQIYKKVISGTKPEALYKVKD 256

Query: 247 LEAQKFVRKCLENVSKRLPARELLLDPFLASND----------------------NASDN 306
              ++FV KCL   S+RL ARELL DPFL  +D                      +A  N
Sbjct: 257 PMVRQFVEKCLATASRRLSARELLKDPFLQVDDLVFCPGDGDYSLMNYLRQPYLEHAYSN 316

Query: 307 EELCL----------SPTPKKSVARRTDMAISG-------------------SMNPKDND 366
             +            +PT  +      D+   G                        ++ 
Sbjct: 317 VSMMSNGLSESIDEDTPTEDRWDCEDDDIKADGIDLFNGHEDEPLGNVDITIKGRKSEDG 376

Query: 367 KVFLKVQIKVKNGKAKNVYFAFDTINDTAIDVATEMVKELEISDWDPLEIAVMIKKEIST 381
            +FL+++I   +G  +N+YF FD   DTA+ VATEMV EL+I+D +   IA MI  E+S 
Sbjct: 377 SIFLRLRIADNDGHVRNIYFPFDIEADTALSVATEMVAELDITDHEVTRIAEMIDGEVSA 436

BLAST of Lcy04g008290 vs. ExPASy TrEMBL
Match: A0A0A0L051 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G332110 PE=4 SV=1)

HSP 1 Score: 936.0 bits (2418), Expect = 7.6e-269
Identity = 492/606 (81.19%), Postives = 534/606 (88.12%), Query Frame = 0

Query: 1   MAQP-IGYAETDPSGRYGRFREILGKGAMKTVYKAFDEVLGMEVAWNQVQLKDVFHSPED 60
           MAQ  IGYAETDPSGRYGRFREILGKGA KTVYK FDEVLG+EVAWNQV LKDVFHSPE+
Sbjct: 1   MAQSNIGYAETDPSGRYGRFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEE 60

Query: 61  LQRLYSEVHLLKNLNHDSIIRFHASWIDTHRRTFNFITEMFTSGSLREYRQKYRNVDIGA 120
           LQRLYSEVHLLKNLNHDSIIRF+  WIDTHRRTFNFITEMFTSG+LREYRQKYRN+DI A
Sbjct: 61  LQRLYSEVHLLKNLNHDSIIRFYTYWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEA 120

Query: 121 IKNWARQILRGLVYLHEHDPPVIHRDIKCDNIFINGHLGQVKIGDLGLAAILHDSQHAHS 180
           IKNWARQIL GLVYLH HDPP+IHRD+KCDN+FINGHLGQVKIGDLGLAAILHDSQHAHS
Sbjct: 121 IKNWARQILHGLVYLHGHDPPIIHRDLKCDNVFINGHLGQVKIGDLGLAAILHDSQHAHS 180

Query: 181 VIGTPEFMAPELYDEEYNELVDVYSFGMCLIEMLTLEYPYSECSNPAQIYKKVTSGKLPN 240
           VIGTPEFMAPELYDEEYNELVDVYSFGMC+IEMLTLEYPYSEC NPAQIYKKVTSGKLPN
Sbjct: 181 VIGTPEFMAPELYDEEYNELVDVYSFGMCMIEMLTLEYPYSECFNPAQIYKKVTSGKLPN 240

Query: 241 AFYKIKGLEAQKFVRKCLENVSKRLPARELLLDPFLASN--DNASDNEELCLSP-TPKKS 300
           AFY+IK LEAQ+FVRKCLENVSKR+ ARELLLDPFLA +  +NAS NEEL  S  +P+KS
Sbjct: 241 AFYEIKDLEAQRFVRKCLENVSKRVSARELLLDPFLAPSNANNASHNEELLSSSLSPEKS 300

Query: 301 V-ARRTDMAISGSMNPKDNDKVFLKVQIKVKNGKAKNVYFAFDTINDTAIDVATEMVKEL 360
           + ARRTD+AISGS+NPKD D +FLKVQIKVKNGK+KNVYFAFD +NDT IDVATEMVKEL
Sbjct: 301 IMARRTDLAISGSINPKD-DSIFLKVQIKVKNGKSKNVYFAFDILNDTTIDVATEMVKEL 360

Query: 361 E-ISDWDPLEIAVMIKKEISTLIPDWEEWNIPKNHHQDSFNY----DVGNDDDDDDDDEN 420
           E ISDWDPLEIAVMI+KEIS+LIPDWEEW +PK  HQDSFNY    D  ND+D+DDDDEN
Sbjct: 361 EIISDWDPLEIAVMIEKEISSLIPDWEEWKLPKIQHQDSFNYEQDHDGDNDNDNDDDDEN 420

Query: 421 NVTPHPFYSC-SSHASSSNSLQGFYSLYDNP-HHFGGRNGTSTATEWFQEDLLIDEDASS 480
             TPHPFY C SSH SSS+SL  FYS  +NP H+FGG   TS  TEWF+ED     D SS
Sbjct: 421 YATPHPFYYCGSSHGSSSDSLHAFYSSRENPNHYFGGMKDTSNTTEWFRED-----DTSS 480

Query: 481 SCSQNSFNYSDVSFDSNNEDDYESDPHLKGREPHNISTTKQPTRFCPTMKIDTHNLRHKD 540
            CS NSFNYSD+SF SNNED+YE D ++KGREP  +STTKQPTRFCPTMKID+H+LRHKD
Sbjct: 481 CCSLNSFNYSDLSFYSNNEDEYEYDSNIKGREPQYVSTTKQPTRFCPTMKIDSHHLRHKD 540

Query: 541 NQIMLDRE-FEIQYRSNNNSHRLTRVKSMVNLRSSETLHRSLVEMLLKKRLFNTVGAMEN 594
           N+I+ +RE FE + RS NNS RLTRVKSMVNLR SETLHR LVEMLLKKRLFNTVGAMEN
Sbjct: 541 NKIIPNREVFESRSRS-NNSPRLTRVKSMVNLR-SETLHRYLVEMLLKKRLFNTVGAMEN 598

BLAST of Lcy04g008290 vs. ExPASy TrEMBL
Match: A0A6J1CQE4 (probable serine/threonine-protein kinase WNK5 OS=Momordica charantia OX=3673 GN=LOC111013783 PE=4 SV=1)

HSP 1 Score: 915.2 bits (2364), Expect = 1.4e-262
Identity = 479/606 (79.04%), Postives = 524/606 (86.47%), Query Frame = 0

Query: 1   MAQPIGYAETDPSGRYGRFREILGKGAMKTVYKAFDEVLGMEVAWNQVQLKDVFHSPEDL 60
           MAQ I Y ETDPSGRYGRFREILGKGAMKTVYKAFDEVLG+EVAWNQV+LKDVFHSPE+L
Sbjct: 1   MAQQIAYVETDPSGRYGRFREILGKGAMKTVYKAFDEVLGIEVAWNQVKLKDVFHSPEEL 60

Query: 61  QRLYSEVHLLKNLNHDSIIRFHASWIDTHRRTFNFITEMFTSGSLREYRQKYRNVDIGAI 120
           QRLYSEVHLLKNLNHDSIIRF+ SWIDTHRRTFNFITEMFTSG+LREYRQKYR +DIGA+
Sbjct: 61  QRLYSEVHLLKNLNHDSIIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRKLDIGAV 120

Query: 121 KNWARQILRGLVYLHEHDPPVIHRDIKCDNIFINGHLGQVKIGDLGLAAILHDSQHAHSV 180
           KNWARQILRGL YLH HDPPVIHRD+KCDNIFINGHLGQVKIGDLGLAAILHDS+HAHSV
Sbjct: 121 KNWARQILRGLAYLHGHDPPVIHRDLKCDNIFINGHLGQVKIGDLGLAAILHDSKHAHSV 180

Query: 181 IGTPEFMAPELYDEEYNELVDVYSFGMCLIEMLTLEYPYSECSNPAQIYKKVTSGKLPNA 240
           IGTPEFMAPELYDEEYNELVDVYSFGMC+IEMLTLEYPYSECSNPAQIYKKVTSGKLPNA
Sbjct: 181 IGTPEFMAPELYDEEYNELVDVYSFGMCVIEMLTLEYPYSECSNPAQIYKKVTSGKLPNA 240

Query: 241 FYKIKGLEAQKFVRKCLENVSKRLPARELLLDPFLASNDNASDNEELCLSP-----TPKK 300
           +Y+I+ LEAQKFVRKCLENVSKRLPA ELLL PFLAS D+ S  EEL  +P      PK+
Sbjct: 241 YYRIEDLEAQKFVRKCLENVSKRLPATELLLHPFLAS-DDVSHEEELPATPPLISHPPKE 300

Query: 301 S---VARRTDMAISGSMNPKDNDKVFLKVQIKVKNGKAKNVYFAFDTINDTAIDVATEMV 360
           +   V RRTDM ISGSMNP+D D VFLKV+IKVK G AKNVYF FDT+ND AIDVATEMV
Sbjct: 301 TAIPVPRRTDMTISGSMNPED-DTVFLKVRIKVKGGNAKNVYFTFDTVNDIAIDVATEMV 360

Query: 361 KELEISDWDPLEIAVMIKKEISTLIPDWEEWN--IPKNHHQDSFNYDVGNDDDDDDDDEN 420
           KELEI DWDPLEIAVMIKKE+S LIP+WEEWN  IPKNHHQDSFNY       +++++E+
Sbjct: 361 KELEIIDWDPLEIAVMIKKEVSKLIPNWEEWNNFIPKNHHQDSFNY-------ENEEEED 420

Query: 421 NVTPHPFYSCSSHASSSNSLQGFYSLYDNPHHFGGRNGTSTATEWFQED-LLIDEDASSS 480
           NVTP+PFYSCSS+ASSSNSLQGFYSLYDNP  +GG N TS A++WFQED +LID DASSS
Sbjct: 421 NVTPNPFYSCSSYASSSNSLQGFYSLYDNPRQWGGMNDTSVASQWFQEDHILIDGDASSS 480

Query: 481 CSQNSFNYSDVSFDSNNED--DYESDPHLKGREPHNISTTKQPTRFCPTMKIDTHNLRHK 540
            S NSFN S++SF SNNED  + E D H KGREPH ISTTKQPTRFCPTM  D H+LRHK
Sbjct: 481 TSMNSFNGSNISFYSNNEDHEEDECDSHFKGREPHCISTTKQPTRFCPTMDPDAHHLRHK 540

Query: 541 DNQIMLDREFEIQYRSNNNSHRLTRVKSMVNLRSSETLHRSLVEMLLKKRLFNTVGAMEN 594
           DN +  DRE E + RS N+SHRLTR +SMV  RS     +SLVEMLL+KRLF TVGA+EN
Sbjct: 541 DNNLS-DRESEGRSRS-NSSHRLTRTRSMVEGRSQ---MKSLVEMLLRKRLFKTVGAVEN 592

BLAST of Lcy04g008290 vs. ExPASy TrEMBL
Match: A0A1S3CJ68 (probable serine/threonine-protein kinase WNK5 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103501457 PE=4 SV=1)

HSP 1 Score: 910.2 bits (2351), Expect = 4.5e-261
Identity = 479/605 (79.17%), Postives = 529/605 (87.44%), Query Frame = 0

Query: 1   MAQP-IGYAETDPSGRYGRFREILGKGAMKTVYKAFDEVLGMEVAWNQVQLKDVFHSPED 60
           MAQ  IGYAETDPSGRYGRFREILGKGA KTVYK FDEVLG+EVAWNQV LKDVFHSPE+
Sbjct: 1   MAQSNIGYAETDPSGRYGRFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEE 60

Query: 61  LQRLYSEVHLLKNLNHDSIIRFHASWIDTHRRTFNFITEMFTSGSLREYRQKYRNVDIGA 120
           L+RLYSEVHLLKNLNH+SIIRF+ SWIDTHRRTFNFITEMFTSG+LREYRQKY+N+DI A
Sbjct: 61  LERLYSEVHLLKNLNHNSIIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYQNIDIEA 120

Query: 121 IKNWARQILRGLVYLHEHDPPVIHRDIKCDNIFINGHLGQVKIGDLGLAAILHDSQHAHS 180
           IKNWARQIL GLVYLH HDPPVIHRD+KCDN+FINGHLGQVKIGDLGLA ILH SQ+AHS
Sbjct: 121 IKNWARQILHGLVYLHGHDPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHGSQNAHS 180

Query: 181 VIGTPEFMAPELYDEEYNELVDVYSFGMCLIEMLTLEYPYSECSNPAQIYKKVTSGKLPN 240
           VIGTPEFMAPELYDEEYNELVDVYSFGMC+IEMLTLEYPY+ECSNPAQIYKKVTSGKLPN
Sbjct: 181 VIGTPEFMAPELYDEEYNELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPN 240

Query: 241 AFYKIKGLEAQKFVRKCLENVSKRLPARELLLDPFLA--SNDNASDNEELCLSP-TPKKS 300
           AFY+IK LEAQ+FVRKCLENVSKR+ ARELLL+PFLA  +++N S NEEL  S  +P+KS
Sbjct: 241 AFYEIKDLEAQRFVRKCLENVSKRVSARELLLEPFLAPSNSNNVSHNEELVSSSLSPEKS 300

Query: 301 V-ARRTDMAISGSMNPKDNDKVFLKVQIKVKNGKAKNVYFAFDTINDTAIDVATEMVKEL 360
           + ARRTD+AISGS+NPKD D +FLKVQIKVKNGK+KNVYFAFD +NDTAIDVATEMVKEL
Sbjct: 301 IMARRTDLAISGSINPKD-DSIFLKVQIKVKNGKSKNVYFAFDILNDTAIDVATEMVKEL 360

Query: 361 E-ISDWDPLEIAVMIKKEISTLIPDWEEWNIPKNHHQDSFNYDV---GNDD-DDDDDDEN 420
           E ISDWDPLEIAVMI+KEIS+LIPDWEEW  P+ HHQDSFNY+    GNDD DDDDDDEN
Sbjct: 361 EIISDWDPLEIAVMIEKEISSLIPDWEEWKFPEIHHQDSFNYEQDHNGNDDNDDDDDDEN 420

Query: 421 NVTPHPFYSC-SSHASSSNSLQGFYSLYDNP-HHFGGRNGTSTATEWFQEDLLIDEDASS 480
             TPHPFY C SSH SSS+SL  FY   +NP HHFGG + TS  TEWF+ED     D SS
Sbjct: 421 YATPHPFYYCGSSHGSSSDSLHDFYPFRENPNHHFGGMD-TSITTEWFRED-----DTSS 480

Query: 481 SCSQNSFNYSDVSFDSNNEDDYESDPHLKGREPHNISTTKQPTRFCPTMKIDTHNLRHKD 540
            CS NSF+YSD+SF SN+ED+YE D ++KGRE   ISTTKQPTRFCPTMKID+H+LRHKD
Sbjct: 481 CCSLNSFDYSDLSFYSNDEDEYEYDSNIKGRESRYISTTKQPTRFCPTMKIDSHHLRHKD 540

Query: 541 NQIMLDREFEIQYRSNNNSHRLTRVKSMVNLRSSETLHRSLVEMLLKKRLFNTVGAMENI 594
            +I+ +RE       +NNS R+TRVKSMVNLR SETLHR LVE LLKKRLFNTVGAMENI
Sbjct: 541 TRIIPNRETFENGSLSNNSPRITRVKSMVNLR-SETLHRYLVETLLKKRLFNTVGAMENI 597

BLAST of Lcy04g008290 vs. ExPASy TrEMBL
Match: A0A1S3CIS3 (probable serine/threonine-protein kinase WNK5 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501457 PE=4 SV=1)

HSP 1 Score: 904.8 bits (2337), Expect = 1.9e-259
Identity = 479/608 (78.78%), Postives = 529/608 (87.01%), Query Frame = 0

Query: 1   MAQP-IGYAETDPSGRYGRFREILGKGAMKTVYKAFDEVLGMEVAWNQVQLKDVFHSPED 60
           MAQ  IGYAETDPSGRYGRFREILGKGA KTVYK FDEVLG+EVAWNQV LKDVFHSPE+
Sbjct: 1   MAQSNIGYAETDPSGRYGRFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEE 60

Query: 61  LQRLYSEVHLLKNLNHDSIIRFHASWIDTHRRTFNFITEMFTSGSLREYRQKYRNVDIGA 120
           L+RLYSEVHLLKNLNH+SIIRF+ SWIDTHRRTFNFITEMFTSG+LREYRQKY+N+DI A
Sbjct: 61  LERLYSEVHLLKNLNHNSIIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYQNIDIEA 120

Query: 121 IKNWARQILRGLVYLHEHDPPVIHRDIKCDNIFINGHLGQVKIGDLGLAAILHDSQHAHS 180
           IKNWARQIL GLVYLH HDPPVIHRD+KCDN+FINGHLGQVKIGDLGLA ILH SQ+AHS
Sbjct: 121 IKNWARQILHGLVYLHGHDPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHGSQNAHS 180

Query: 181 VIGTPEFMAPELYDEEYNELVDVYSFGMCLIEMLTLEYPYSECSNPAQIYKKVTS---GK 240
           VIGTPEFMAPELYDEEYNELVDVYSFGMC+IEMLTLEYPY+ECSNPAQIYKKVTS   GK
Sbjct: 181 VIGTPEFMAPELYDEEYNELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSKLQGK 240

Query: 241 LPNAFYKIKGLEAQKFVRKCLENVSKRLPARELLLDPFLA--SNDNASDNEELCLSP-TP 300
           LPNAFY+IK LEAQ+FVRKCLENVSKR+ ARELLL+PFLA  +++N S NEEL  S  +P
Sbjct: 241 LPNAFYEIKDLEAQRFVRKCLENVSKRVSARELLLEPFLAPSNSNNVSHNEELVSSSLSP 300

Query: 301 KKSV-ARRTDMAISGSMNPKDNDKVFLKVQIKVKNGKAKNVYFAFDTINDTAIDVATEMV 360
           +KS+ ARRTD+AISGS+NPKD D +FLKVQIKVKNGK+KNVYFAFD +NDTAIDVATEMV
Sbjct: 301 EKSIMARRTDLAISGSINPKD-DSIFLKVQIKVKNGKSKNVYFAFDILNDTAIDVATEMV 360

Query: 361 KELE-ISDWDPLEIAVMIKKEISTLIPDWEEWNIPKNHHQDSFNYDV---GNDD-DDDDD 420
           KELE ISDWDPLEIAVMI+KEIS+LIPDWEEW  P+ HHQDSFNY+    GNDD DDDDD
Sbjct: 361 KELEIISDWDPLEIAVMIEKEISSLIPDWEEWKFPEIHHQDSFNYEQDHNGNDDNDDDDD 420

Query: 421 DENNVTPHPFYSC-SSHASSSNSLQGFYSLYDNP-HHFGGRNGTSTATEWFQEDLLIDED 480
           DEN  TPHPFY C SSH SSS+SL  FY   +NP HHFGG + TS  TEWF+ED     D
Sbjct: 421 DENYATPHPFYYCGSSHGSSSDSLHDFYPFRENPNHHFGGMD-TSITTEWFRED-----D 480

Query: 481 ASSSCSQNSFNYSDVSFDSNNEDDYESDPHLKGREPHNISTTKQPTRFCPTMKIDTHNLR 540
            SS CS NSF+YSD+SF SN+ED+YE D ++KGRE   ISTTKQPTRFCPTMKID+H+LR
Sbjct: 481 TSSCCSLNSFDYSDLSFYSNDEDEYEYDSNIKGRESRYISTTKQPTRFCPTMKIDSHHLR 540

Query: 541 HKDNQIMLDREFEIQYRSNNNSHRLTRVKSMVNLRSSETLHRSLVEMLLKKRLFNTVGAM 594
           HKD +I+ +RE       +NNS R+TRVKSMVNLR SETLHR LVE LLKKRLFNTVGAM
Sbjct: 541 HKDTRIIPNRETFENGSLSNNSPRITRVKSMVNLR-SETLHRYLVETLLKKRLFNTVGAM 600

BLAST of Lcy04g008290 vs. ExPASy TrEMBL
Match: A0A5A7SMB2 (Putative serine/threonine-protein kinase WNK5 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1220G00080 PE=4 SV=1)

HSP 1 Score: 895.2 bits (2312), Expect = 1.5e-256
Identity = 475/605 (78.51%), Postives = 522/605 (86.28%), Query Frame = 0

Query: 1   MAQP-IGYAETDPSGRYGRFREILGKGAMKTVYKAFDEVLGMEVAWNQVQLKDVFHSPED 60
           MAQ  IGYAETDPSGRYGRFREILGKGA KTVYK FDEVLG+EVAWNQV LKDVFHSPE+
Sbjct: 1   MAQSNIGYAETDPSGRYGRFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEE 60

Query: 61  LQRLYSEVHLLKNLNHDSIIRFHASWIDTHRRTFNFITEMFTSGSLREYRQKYRNVDIGA 120
           L+RLYSEVHLLKNLNH+SIIRF+ SWIDTHRRTFNFITEMFTSG+LREYRQKY+N+DI A
Sbjct: 61  LERLYSEVHLLKNLNHNSIIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYQNIDIEA 120

Query: 121 IKNWARQILRGLVYLHEHDPPVIHRDIKCDNIFINGHLGQVKIGDLGLAAILHDSQHAHS 180
           IKNWARQIL GLVYLH HDPPVIHRD+KCDN+FINGHLGQVKIGDLGLA ILH SQHAHS
Sbjct: 121 IKNWARQILHGLVYLHGHDPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHGSQHAHS 180

Query: 181 VIGTPEFMAPELYDEEYNELVDVYSFGMCLIEMLTLEYPYSECSNPAQIYKKVTSGKLPN 240
           VIGTPEFMAPELYDEEYNELVDVYSFGMC+IEMLTLEYPY+ECSNPAQIYKKVTSGKLPN
Sbjct: 181 VIGTPEFMAPELYDEEYNELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPN 240

Query: 241 AFYKIKGLEAQKFVRKCLENVSKRLPARELLLDPFLA--SNDNASDNEELCLSP-TPKKS 300
           AFY+IK LEAQ+FVRKCLENVSKR+ ARELLL+PFLA  +++N S NEEL  S  +P+KS
Sbjct: 241 AFYEIKDLEAQRFVRKCLENVSKRVSARELLLEPFLAPSNSNNVSHNEELVSSSLSPEKS 300

Query: 301 V-ARRTDMAISGSMNPKDNDKVFLKVQIKVKNGKAKNVYFAFDTINDTAIDVATEMVKEL 360
           + ARRTD+AISGS+NPKD D +FLKVQI       KNVYFAFD +NDTAIDVATEMVKEL
Sbjct: 301 IMARRTDLAISGSINPKD-DSIFLKVQI-------KNVYFAFDILNDTAIDVATEMVKEL 360

Query: 361 E-ISDWDPLEIAVMIKKEISTLIPDWEEWNIPKNHHQDSFNYDV---GNDD-DDDDDDEN 420
           E ISDWDPLEIAVMI+KEIS+LIPDWEEW  P+ HHQDSFNY+    GNDD DDDDDDEN
Sbjct: 361 EIISDWDPLEIAVMIEKEISSLIPDWEEWKFPEIHHQDSFNYEQDHNGNDDNDDDDDDEN 420

Query: 421 NVTPHPFYSC-SSHASSSNSLQGFYSLYDNP-HHFGGRNGTSTATEWFQEDLLIDEDASS 480
             TPHPFY C SSH SSS+SL  FY   +NP HHFGG + TS  TEWF+ED     D SS
Sbjct: 421 YATPHPFYYCGSSHGSSSDSLHDFYPFRENPNHHFGGMD-TSITTEWFRED-----DTSS 480

Query: 481 SCSQNSFNYSDVSFDSNNEDDYESDPHLKGREPHNISTTKQPTRFCPTMKIDTHNLRHKD 540
            CS NSF+YSD+SF SN+ED+YE D ++KGRE   ISTTKQPTRFCPTMKID+H+LRHKD
Sbjct: 481 CCSLNSFDYSDLSFYSNDEDEYEYDSNIKGRESRYISTTKQPTRFCPTMKIDSHHLRHKD 540

Query: 541 NQIMLDREFEIQYRSNNNSHRLTRVKSMVNLRSSETLHRSLVEMLLKKRLFNTVGAMENI 594
            +I+ +RE       +NNS R+TRVKSMVNLR SETLHR LVE LLKKRLFNTVGAMENI
Sbjct: 541 TRIIPNRETFENGSLSNNSPRITRVKSMVNLR-SETLHRYLVETLLKKRLFNTVGAMENI 590

BLAST of Lcy04g008290 vs. NCBI nr
Match: XP_004142734.2 (probable serine/threonine-protein kinase WNK5 [Cucumis sativus] >KGN54444.1 hypothetical protein Csa_012775 [Cucumis sativus])

HSP 1 Score: 936.0 bits (2418), Expect = 1.6e-268
Identity = 492/606 (81.19%), Postives = 534/606 (88.12%), Query Frame = 0

Query: 1   MAQP-IGYAETDPSGRYGRFREILGKGAMKTVYKAFDEVLGMEVAWNQVQLKDVFHSPED 60
           MAQ  IGYAETDPSGRYGRFREILGKGA KTVYK FDEVLG+EVAWNQV LKDVFHSPE+
Sbjct: 1   MAQSNIGYAETDPSGRYGRFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEE 60

Query: 61  LQRLYSEVHLLKNLNHDSIIRFHASWIDTHRRTFNFITEMFTSGSLREYRQKYRNVDIGA 120
           LQRLYSEVHLLKNLNHDSIIRF+  WIDTHRRTFNFITEMFTSG+LREYRQKYRN+DI A
Sbjct: 61  LQRLYSEVHLLKNLNHDSIIRFYTYWIDTHRRTFNFITEMFTSGTLREYRQKYRNIDIEA 120

Query: 121 IKNWARQILRGLVYLHEHDPPVIHRDIKCDNIFINGHLGQVKIGDLGLAAILHDSQHAHS 180
           IKNWARQIL GLVYLH HDPP+IHRD+KCDN+FINGHLGQVKIGDLGLAAILHDSQHAHS
Sbjct: 121 IKNWARQILHGLVYLHGHDPPIIHRDLKCDNVFINGHLGQVKIGDLGLAAILHDSQHAHS 180

Query: 181 VIGTPEFMAPELYDEEYNELVDVYSFGMCLIEMLTLEYPYSECSNPAQIYKKVTSGKLPN 240
           VIGTPEFMAPELYDEEYNELVDVYSFGMC+IEMLTLEYPYSEC NPAQIYKKVTSGKLPN
Sbjct: 181 VIGTPEFMAPELYDEEYNELVDVYSFGMCMIEMLTLEYPYSECFNPAQIYKKVTSGKLPN 240

Query: 241 AFYKIKGLEAQKFVRKCLENVSKRLPARELLLDPFLASN--DNASDNEELCLSP-TPKKS 300
           AFY+IK LEAQ+FVRKCLENVSKR+ ARELLLDPFLA +  +NAS NEEL  S  +P+KS
Sbjct: 241 AFYEIKDLEAQRFVRKCLENVSKRVSARELLLDPFLAPSNANNASHNEELLSSSLSPEKS 300

Query: 301 V-ARRTDMAISGSMNPKDNDKVFLKVQIKVKNGKAKNVYFAFDTINDTAIDVATEMVKEL 360
           + ARRTD+AISGS+NPKD D +FLKVQIKVKNGK+KNVYFAFD +NDT IDVATEMVKEL
Sbjct: 301 IMARRTDLAISGSINPKD-DSIFLKVQIKVKNGKSKNVYFAFDILNDTTIDVATEMVKEL 360

Query: 361 E-ISDWDPLEIAVMIKKEISTLIPDWEEWNIPKNHHQDSFNY----DVGNDDDDDDDDEN 420
           E ISDWDPLEIAVMI+KEIS+LIPDWEEW +PK  HQDSFNY    D  ND+D+DDDDEN
Sbjct: 361 EIISDWDPLEIAVMIEKEISSLIPDWEEWKLPKIQHQDSFNYEQDHDGDNDNDNDDDDEN 420

Query: 421 NVTPHPFYSC-SSHASSSNSLQGFYSLYDNP-HHFGGRNGTSTATEWFQEDLLIDEDASS 480
             TPHPFY C SSH SSS+SL  FYS  +NP H+FGG   TS  TEWF+ED     D SS
Sbjct: 421 YATPHPFYYCGSSHGSSSDSLHAFYSSRENPNHYFGGMKDTSNTTEWFRED-----DTSS 480

Query: 481 SCSQNSFNYSDVSFDSNNEDDYESDPHLKGREPHNISTTKQPTRFCPTMKIDTHNLRHKD 540
            CS NSFNYSD+SF SNNED+YE D ++KGREP  +STTKQPTRFCPTMKID+H+LRHKD
Sbjct: 481 CCSLNSFNYSDLSFYSNNEDEYEYDSNIKGREPQYVSTTKQPTRFCPTMKIDSHHLRHKD 540

Query: 541 NQIMLDRE-FEIQYRSNNNSHRLTRVKSMVNLRSSETLHRSLVEMLLKKRLFNTVGAMEN 594
           N+I+ +RE FE + RS NNS RLTRVKSMVNLR SETLHR LVEMLLKKRLFNTVGAMEN
Sbjct: 541 NKIIPNREVFESRSRS-NNSPRLTRVKSMVNLR-SETLHRYLVEMLLKKRLFNTVGAMEN 598

BLAST of Lcy04g008290 vs. NCBI nr
Match: XP_038883353.1 (probable serine/threonine-protein kinase WNK5 [Benincasa hispida])

HSP 1 Score: 934.1 bits (2413), Expect = 5.9e-268
Identity = 485/592 (81.93%), Postives = 523/592 (88.34%), Query Frame = 0

Query: 11  DPSGRYGRFREILGKGAMKTVYKAFDEVLGMEVAWNQVQLKDVFHSPEDLQRLYSEVHLL 70
           DPSGRYGRFREILGKGAMKTVYK FDEVLG+EVAWNQV LKDVFHSPE+LQRLYSEVHLL
Sbjct: 5   DPSGRYGRFREILGKGAMKTVYKGFDEVLGIEVAWNQVYLKDVFHSPEELQRLYSEVHLL 64

Query: 71  KNLNHDSIIRFHASWIDTHRRTFNFITEMFTSGSLREYRQKYRNVDIGAIKNWARQILRG 130
           KNL+HDSIIRFHASWIDTH RTFNFITEMFTSG+LREYRQKYRN+DI AIKNWARQIL G
Sbjct: 65  KNLDHDSIIRFHASWIDTHHRTFNFITEMFTSGTLREYRQKYRNIDIDAIKNWARQILHG 124

Query: 131 LVYLHEHDPPVIHRDIKCDNIFINGHLGQVKIGDLGLAAILHDSQHAHSVIGTPEFMAPE 190
           LVYLH H+PPVIHRD+KCDN+FINGHLGQVKIGDLGLA ILHDSQHAHSVIGTPEFMAPE
Sbjct: 125 LVYLHGHNPPVIHRDLKCDNLFINGHLGQVKIGDLGLATILHDSQHAHSVIGTPEFMAPE 184

Query: 191 LYDEEYNELVDVYSFGMCLIEMLTLEYPYSECSNPAQIYKKVTSGKLPNAFYKIKGLEAQ 250
           LYDEEYNELVDVYSFGMC+IEMLTLEYPYSECSNPAQIYKKVTSGKLPNAFYKIK  EAQ
Sbjct: 185 LYDEEYNELVDVYSFGMCVIEMLTLEYPYSECSNPAQIYKKVTSGKLPNAFYKIKDFEAQ 244

Query: 251 KFVRKCLENVSKRLPARELLLDPFLA---SNDNASDNEELCLSP-TPKKSVARRTDMAIS 310
           +FVRKCLENVSKR+PARELL DPFLA   +N+ +SDNE+L  S  +PKKS+ARRTDMAIS
Sbjct: 245 RFVRKCLENVSKRVPARELLFDPFLAPSNNNNGSSDNEDLLSSSLSPKKSMARRTDMAIS 304

Query: 311 GSMNPKDNDKVFLKVQIKVKNGKAKNVYFAFDTINDTAIDVATEMVKELE-ISDWDPLEI 370
           GSMNP+D D +FLKVQIKV NGK+KNVYFAFD INDTAIDVATEMVKELE ISDWDPLEI
Sbjct: 305 GSMNPED-DSIFLKVQIKVTNGKSKNVYFAFDPINDTAIDVATEMVKELEIISDWDPLEI 364

Query: 371 AVMIKKEISTLIPDWEEWNIPKNHHQDSFNY--DVGNDDDDDDDDENNVTPHPFYSCSSH 430
           AVMIKKEISTLIPDWEEW +PKNHHQDSFNY  D  +D+D+DDDD+N  TPHPFY CSSH
Sbjct: 365 AVMIKKEISTLIPDWEEWKLPKNHHQDSFNYEQDDNDDNDNDDDDKNFETPHPFYCCSSH 424

Query: 431 ASSSNSLQGFYSLYDNP-HHFGGRNGTSTATEWFQEDLL-IDEDASSSCSQNSFNYSDVS 490
            SSSNSLQ FYS Y+NP HHFGG   TST TEWFQED+L ID+D SSSCS NSFNYSD+S
Sbjct: 425 GSSSNSLQDFYSFYENPNHHFGGTKETST-TEWFQEDILNIDDDTSSSCSLNSFNYSDIS 484

Query: 491 FDSNNEDDYESDPHLKGREPHNISTTKQPTRFCPTMKIDTHNLRHKDNQIMLDREFEIQY 550
           F SNNED+++S  ++KGREP  ISTTKQPTRFCP MK             M+DRE     
Sbjct: 485 FYSNNEDEHDS--NIKGREPQYISTTKQPTRFCPIMK-------------MVDREIIKSQ 544

Query: 551 RSNNNSHRLTRVKSMVNLRSSETLHRSLVEMLLKKRLFNTVGAMENIGYQKP 594
             +NNSHRLTRVKSMVNLR SETLHRSL+EMLLKKRLFNTVGAMENIGYQ+P
Sbjct: 545 SRSNNSHRLTRVKSMVNLR-SETLHRSLIEMLLKKRLFNTVGAMENIGYQEP 578

BLAST of Lcy04g008290 vs. NCBI nr
Match: XP_022143995.1 (probable serine/threonine-protein kinase WNK5 [Momordica charantia])

HSP 1 Score: 915.2 bits (2364), Expect = 2.9e-262
Identity = 479/606 (79.04%), Postives = 524/606 (86.47%), Query Frame = 0

Query: 1   MAQPIGYAETDPSGRYGRFREILGKGAMKTVYKAFDEVLGMEVAWNQVQLKDVFHSPEDL 60
           MAQ I Y ETDPSGRYGRFREILGKGAMKTVYKAFDEVLG+EVAWNQV+LKDVFHSPE+L
Sbjct: 1   MAQQIAYVETDPSGRYGRFREILGKGAMKTVYKAFDEVLGIEVAWNQVKLKDVFHSPEEL 60

Query: 61  QRLYSEVHLLKNLNHDSIIRFHASWIDTHRRTFNFITEMFTSGSLREYRQKYRNVDIGAI 120
           QRLYSEVHLLKNLNHDSIIRF+ SWIDTHRRTFNFITEMFTSG+LREYRQKYR +DIGA+
Sbjct: 61  QRLYSEVHLLKNLNHDSIIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYRKLDIGAV 120

Query: 121 KNWARQILRGLVYLHEHDPPVIHRDIKCDNIFINGHLGQVKIGDLGLAAILHDSQHAHSV 180
           KNWARQILRGL YLH HDPPVIHRD+KCDNIFINGHLGQVKIGDLGLAAILHDS+HAHSV
Sbjct: 121 KNWARQILRGLAYLHGHDPPVIHRDLKCDNIFINGHLGQVKIGDLGLAAILHDSKHAHSV 180

Query: 181 IGTPEFMAPELYDEEYNELVDVYSFGMCLIEMLTLEYPYSECSNPAQIYKKVTSGKLPNA 240
           IGTPEFMAPELYDEEYNELVDVYSFGMC+IEMLTLEYPYSECSNPAQIYKKVTSGKLPNA
Sbjct: 181 IGTPEFMAPELYDEEYNELVDVYSFGMCVIEMLTLEYPYSECSNPAQIYKKVTSGKLPNA 240

Query: 241 FYKIKGLEAQKFVRKCLENVSKRLPARELLLDPFLASNDNASDNEELCLSP-----TPKK 300
           +Y+I+ LEAQKFVRKCLENVSKRLPA ELLL PFLAS D+ S  EEL  +P      PK+
Sbjct: 241 YYRIEDLEAQKFVRKCLENVSKRLPATELLLHPFLAS-DDVSHEEELPATPPLISHPPKE 300

Query: 301 S---VARRTDMAISGSMNPKDNDKVFLKVQIKVKNGKAKNVYFAFDTINDTAIDVATEMV 360
           +   V RRTDM ISGSMNP+D D VFLKV+IKVK G AKNVYF FDT+ND AIDVATEMV
Sbjct: 301 TAIPVPRRTDMTISGSMNPED-DTVFLKVRIKVKGGNAKNVYFTFDTVNDIAIDVATEMV 360

Query: 361 KELEISDWDPLEIAVMIKKEISTLIPDWEEWN--IPKNHHQDSFNYDVGNDDDDDDDDEN 420
           KELEI DWDPLEIAVMIKKE+S LIP+WEEWN  IPKNHHQDSFNY       +++++E+
Sbjct: 361 KELEIIDWDPLEIAVMIKKEVSKLIPNWEEWNNFIPKNHHQDSFNY-------ENEEEED 420

Query: 421 NVTPHPFYSCSSHASSSNSLQGFYSLYDNPHHFGGRNGTSTATEWFQED-LLIDEDASSS 480
           NVTP+PFYSCSS+ASSSNSLQGFYSLYDNP  +GG N TS A++WFQED +LID DASSS
Sbjct: 421 NVTPNPFYSCSSYASSSNSLQGFYSLYDNPRQWGGMNDTSVASQWFQEDHILIDGDASSS 480

Query: 481 CSQNSFNYSDVSFDSNNED--DYESDPHLKGREPHNISTTKQPTRFCPTMKIDTHNLRHK 540
            S NSFN S++SF SNNED  + E D H KGREPH ISTTKQPTRFCPTM  D H+LRHK
Sbjct: 481 TSMNSFNGSNISFYSNNEDHEEDECDSHFKGREPHCISTTKQPTRFCPTMDPDAHHLRHK 540

Query: 541 DNQIMLDREFEIQYRSNNNSHRLTRVKSMVNLRSSETLHRSLVEMLLKKRLFNTVGAMEN 594
           DN +  DRE E + RS N+SHRLTR +SMV  RS     +SLVEMLL+KRLF TVGA+EN
Sbjct: 541 DNNLS-DRESEGRSRS-NSSHRLTRTRSMVEGRSQ---MKSLVEMLLRKRLFKTVGAVEN 592

BLAST of Lcy04g008290 vs. NCBI nr
Match: XP_008463254.1 (PREDICTED: probable serine/threonine-protein kinase WNK5 isoform X2 [Cucumis melo])

HSP 1 Score: 910.2 bits (2351), Expect = 9.2e-261
Identity = 479/605 (79.17%), Postives = 529/605 (87.44%), Query Frame = 0

Query: 1   MAQP-IGYAETDPSGRYGRFREILGKGAMKTVYKAFDEVLGMEVAWNQVQLKDVFHSPED 60
           MAQ  IGYAETDPSGRYGRFREILGKGA KTVYK FDEVLG+EVAWNQV LKDVFHSPE+
Sbjct: 1   MAQSNIGYAETDPSGRYGRFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEE 60

Query: 61  LQRLYSEVHLLKNLNHDSIIRFHASWIDTHRRTFNFITEMFTSGSLREYRQKYRNVDIGA 120
           L+RLYSEVHLLKNLNH+SIIRF+ SWIDTHRRTFNFITEMFTSG+LREYRQKY+N+DI A
Sbjct: 61  LERLYSEVHLLKNLNHNSIIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYQNIDIEA 120

Query: 121 IKNWARQILRGLVYLHEHDPPVIHRDIKCDNIFINGHLGQVKIGDLGLAAILHDSQHAHS 180
           IKNWARQIL GLVYLH HDPPVIHRD+KCDN+FINGHLGQVKIGDLGLA ILH SQ+AHS
Sbjct: 121 IKNWARQILHGLVYLHGHDPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHGSQNAHS 180

Query: 181 VIGTPEFMAPELYDEEYNELVDVYSFGMCLIEMLTLEYPYSECSNPAQIYKKVTSGKLPN 240
           VIGTPEFMAPELYDEEYNELVDVYSFGMC+IEMLTLEYPY+ECSNPAQIYKKVTSGKLPN
Sbjct: 181 VIGTPEFMAPELYDEEYNELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSGKLPN 240

Query: 241 AFYKIKGLEAQKFVRKCLENVSKRLPARELLLDPFLA--SNDNASDNEELCLSP-TPKKS 300
           AFY+IK LEAQ+FVRKCLENVSKR+ ARELLL+PFLA  +++N S NEEL  S  +P+KS
Sbjct: 241 AFYEIKDLEAQRFVRKCLENVSKRVSARELLLEPFLAPSNSNNVSHNEELVSSSLSPEKS 300

Query: 301 V-ARRTDMAISGSMNPKDNDKVFLKVQIKVKNGKAKNVYFAFDTINDTAIDVATEMVKEL 360
           + ARRTD+AISGS+NPKD D +FLKVQIKVKNGK+KNVYFAFD +NDTAIDVATEMVKEL
Sbjct: 301 IMARRTDLAISGSINPKD-DSIFLKVQIKVKNGKSKNVYFAFDILNDTAIDVATEMVKEL 360

Query: 361 E-ISDWDPLEIAVMIKKEISTLIPDWEEWNIPKNHHQDSFNYDV---GNDD-DDDDDDEN 420
           E ISDWDPLEIAVMI+KEIS+LIPDWEEW  P+ HHQDSFNY+    GNDD DDDDDDEN
Sbjct: 361 EIISDWDPLEIAVMIEKEISSLIPDWEEWKFPEIHHQDSFNYEQDHNGNDDNDDDDDDEN 420

Query: 421 NVTPHPFYSC-SSHASSSNSLQGFYSLYDNP-HHFGGRNGTSTATEWFQEDLLIDEDASS 480
             TPHPFY C SSH SSS+SL  FY   +NP HHFGG + TS  TEWF+ED     D SS
Sbjct: 421 YATPHPFYYCGSSHGSSSDSLHDFYPFRENPNHHFGGMD-TSITTEWFRED-----DTSS 480

Query: 481 SCSQNSFNYSDVSFDSNNEDDYESDPHLKGREPHNISTTKQPTRFCPTMKIDTHNLRHKD 540
            CS NSF+YSD+SF SN+ED+YE D ++KGRE   ISTTKQPTRFCPTMKID+H+LRHKD
Sbjct: 481 CCSLNSFDYSDLSFYSNDEDEYEYDSNIKGRESRYISTTKQPTRFCPTMKIDSHHLRHKD 540

Query: 541 NQIMLDREFEIQYRSNNNSHRLTRVKSMVNLRSSETLHRSLVEMLLKKRLFNTVGAMENI 594
            +I+ +RE       +NNS R+TRVKSMVNLR SETLHR LVE LLKKRLFNTVGAMENI
Sbjct: 541 TRIIPNRETFENGSLSNNSPRITRVKSMVNLR-SETLHRYLVETLLKKRLFNTVGAMENI 597

BLAST of Lcy04g008290 vs. NCBI nr
Match: XP_008463253.1 (PREDICTED: probable serine/threonine-protein kinase WNK5 isoform X1 [Cucumis melo])

HSP 1 Score: 904.8 bits (2337), Expect = 3.9e-259
Identity = 479/608 (78.78%), Postives = 529/608 (87.01%), Query Frame = 0

Query: 1   MAQP-IGYAETDPSGRYGRFREILGKGAMKTVYKAFDEVLGMEVAWNQVQLKDVFHSPED 60
           MAQ  IGYAETDPSGRYGRFREILGKGA KTVYK FDEVLG+EVAWNQV LKDVFHSPE+
Sbjct: 1   MAQSNIGYAETDPSGRYGRFREILGKGATKTVYKGFDEVLGIEVAWNQVHLKDVFHSPEE 60

Query: 61  LQRLYSEVHLLKNLNHDSIIRFHASWIDTHRRTFNFITEMFTSGSLREYRQKYRNVDIGA 120
           L+RLYSEVHLLKNLNH+SIIRF+ SWIDTHRRTFNFITEMFTSG+LREYRQKY+N+DI A
Sbjct: 61  LERLYSEVHLLKNLNHNSIIRFYTSWIDTHRRTFNFITEMFTSGTLREYRQKYQNIDIEA 120

Query: 121 IKNWARQILRGLVYLHEHDPPVIHRDIKCDNIFINGHLGQVKIGDLGLAAILHDSQHAHS 180
           IKNWARQIL GLVYLH HDPPVIHRD+KCDN+FINGHLGQVKIGDLGLA ILH SQ+AHS
Sbjct: 121 IKNWARQILHGLVYLHGHDPPVIHRDLKCDNVFINGHLGQVKIGDLGLATILHGSQNAHS 180

Query: 181 VIGTPEFMAPELYDEEYNELVDVYSFGMCLIEMLTLEYPYSECSNPAQIYKKVTS---GK 240
           VIGTPEFMAPELYDEEYNELVDVYSFGMC+IEMLTLEYPY+ECSNPAQIYKKVTS   GK
Sbjct: 181 VIGTPEFMAPELYDEEYNELVDVYSFGMCMIEMLTLEYPYNECSNPAQIYKKVTSKLQGK 240

Query: 241 LPNAFYKIKGLEAQKFVRKCLENVSKRLPARELLLDPFLA--SNDNASDNEELCLSP-TP 300
           LPNAFY+IK LEAQ+FVRKCLENVSKR+ ARELLL+PFLA  +++N S NEEL  S  +P
Sbjct: 241 LPNAFYEIKDLEAQRFVRKCLENVSKRVSARELLLEPFLAPSNSNNVSHNEELVSSSLSP 300

Query: 301 KKSV-ARRTDMAISGSMNPKDNDKVFLKVQIKVKNGKAKNVYFAFDTINDTAIDVATEMV 360
           +KS+ ARRTD+AISGS+NPKD D +FLKVQIKVKNGK+KNVYFAFD +NDTAIDVATEMV
Sbjct: 301 EKSIMARRTDLAISGSINPKD-DSIFLKVQIKVKNGKSKNVYFAFDILNDTAIDVATEMV 360

Query: 361 KELE-ISDWDPLEIAVMIKKEISTLIPDWEEWNIPKNHHQDSFNYDV---GNDD-DDDDD 420
           KELE ISDWDPLEIAVMI+KEIS+LIPDWEEW  P+ HHQDSFNY+    GNDD DDDDD
Sbjct: 361 KELEIISDWDPLEIAVMIEKEISSLIPDWEEWKFPEIHHQDSFNYEQDHNGNDDNDDDDD 420

Query: 421 DENNVTPHPFYSC-SSHASSSNSLQGFYSLYDNP-HHFGGRNGTSTATEWFQEDLLIDED 480
           DEN  TPHPFY C SSH SSS+SL  FY   +NP HHFGG + TS  TEWF+ED     D
Sbjct: 421 DENYATPHPFYYCGSSHGSSSDSLHDFYPFRENPNHHFGGMD-TSITTEWFRED-----D 480

Query: 481 ASSSCSQNSFNYSDVSFDSNNEDDYESDPHLKGREPHNISTTKQPTRFCPTMKIDTHNLR 540
            SS CS NSF+YSD+SF SN+ED+YE D ++KGRE   ISTTKQPTRFCPTMKID+H+LR
Sbjct: 481 TSSCCSLNSFDYSDLSFYSNDEDEYEYDSNIKGRESRYISTTKQPTRFCPTMKIDSHHLR 540

Query: 541 HKDNQIMLDREFEIQYRSNNNSHRLTRVKSMVNLRSSETLHRSLVEMLLKKRLFNTVGAM 594
           HKD +I+ +RE       +NNS R+TRVKSMVNLR SETLHR LVE LLKKRLFNTVGAM
Sbjct: 541 HKDTRIIPNRETFENGSLSNNSPRITRVKSMVNLR-SETLHRYLVETLLKKRLFNTVGAM 600

BLAST of Lcy04g008290 vs. TAIR 10
Match: AT5G58350.1 (with no lysine (K) kinase 4 )

HSP 1 Score: 547.0 bits (1408), Expect = 1.9e-155
Identity = 310/604 (51.32%), Postives = 399/604 (66.06%), Query Frame = 0

Query: 1   MAQPIGYAETDPSGRYGRFREILGKGAMKTVYKAFDEVLGMEVAWNQVQLKDVFHSPEDL 60
           M Q   Y ETDP+GRYGRF EILG+GAMKTVYKA DE LG+EVAW+QV+LK+V  S  DL
Sbjct: 3   MNQVAEYVETDPTGRYGRFAEILGRGAMKTVYKAIDEKLGIEVAWSQVKLKEVLRSSVDL 62

Query: 61  QRLYSEVHLLKNLNHDSIIRFHASWIDTHRRTFNFITEMFTSGSLREYRQKYRNVDIGAI 120
           QRLYSEVHLL  LNH SIIRF+ SWID H  T NFITE+FTSG+LR+Y+ KY  +DI AI
Sbjct: 63  QRLYSEVHLLSTLNHKSIIRFYTSWIDVHNHTLNFITELFTSGTLRQYKNKYLRIDIRAI 122

Query: 121 KNWARQILRGLVYLHEHDPPVIHRDIKCDNIFINGHLGQVKIGDLGLAAILHDSQHAHSV 180
           K+WARQIL GLVYLHEHDPPVIHRD+KCDNIF+NGHLGQVKIGDLGLA +L D   AHS+
Sbjct: 123 KSWARQILEGLVYLHEHDPPVIHRDLKCDNIFVNGHLGQVKIGDLGLARMLRDCHSAHSI 182

Query: 181 IGTPEFMAPELYDEEYNELVDVYSFGMCLIEMLTLEYPYSECSNPAQIYKKVTSGKLPNA 240
           IGTPEFMAPELY+E YNEL+DVYSFGMC +EM+T E+PYSEC++PAQIYKKV  GKLP A
Sbjct: 183 IGTPEFMAPELYEENYNELIDVYSFGMCFLEMITSEFPYSECNHPAQIYKKVVGGKLPGA 242

Query: 241 FYKIKGLEAQKFVRKCLENVSKRLPARELLLDPFLASND------NASDNEELCLSPTPK 300
           FY++  +EAQ+F+ KCL + SKR+ A+ELL DPFLAS++      + + N +  L+    
Sbjct: 243 FYRVGDIEAQRFIGKCLVSASKRVSAKELLQDPFLASDESWMVYTSGAGNPKPFLNENEM 302

Query: 301 KSV-----ARRTDMAISGSMNPKDNDKVFLKVQIKVKNGKAKNVYFAFDTINDTAIDVAT 360
            ++       RT+M+I+G +  +DN K+ L+VQI   NG A NV+F FD +NDT+IDVA 
Sbjct: 303 DTLKLEDDELRTEMSIAGKLGAEDN-KIDLEVQIAYDNGLANNVFFPFDIMNDTSIDVAK 362

Query: 361 EMVKELEISDWDPLEIAVMIKKEISTLIPDWEEWNIPKNHHQDSFNYDVGNDDDDDDDDE 420
           EMVKELEI DW+P+EIA MI   IS+L+ DW+                   +DD+   D 
Sbjct: 363 EMVKELEIIDWEPVEIAKMIDGAISSLVSDWKY-----------------EEDDETPHDH 422

Query: 421 NNVTPHPFYSCSSHASSSNSLQGFYSLYDNPHHFGGRNGTSTATEWFQEDLLIDEDASSS 480
           +      F+S SSHASSS +    Y                   +W Q+DL   ++  S 
Sbjct: 423 HRHRTDSFHSSSSHASSSQASLSNYM-------------ARGLQDWVQDDL--HDETYSQ 482

Query: 481 CSQNSFNYSDVSFDSNNEDDYESDPHLKGREPHNISTTKQPTRFCPTMKIDTHNLRHKDN 540
            S +S +YS++++ +   D+Y S   +  R  HN+      TRFCP  +  +H    + N
Sbjct: 483 SSSHSGSYSNLNYIA--VDEYSSQSPVMSR-THNM------TRFCP--EESSHLQSGQAN 542

Query: 541 QIMLDREFEIQYRSNNNSHRLTRVKSMVNLRSSETLHRSLVEMLLKKRLFNTVGAMENIG 594
                        S+N +  LTR +S+V+++  + LHRS  E   K+RLF TVG +E +G
Sbjct: 543 AYAASSSTNRSLASDNRT--LTRNRSLVDVQ-RQLLHRSPGEEARKRRLFKTVGDVETVG 559

BLAST of Lcy04g008290 vs. TAIR 10
Match: AT3G51630.1 (with no lysine (K) kinase 5 )

HSP 1 Score: 544.7 bits (1402), Expect = 9.5e-155
Identity = 297/502 (59.16%), Postives = 360/502 (71.71%), Query Frame = 0

Query: 5   IGYAETDPSGRYGRFREILGKGAMKTVYKAFDEVLGMEVAWNQVQLKDVFHSPEDLQRLY 64
           I Y ETDPSGRYGRFRE+LGKGAMKTVYKAFD+VLGMEVAWNQV+L +VF SPE LQRLY
Sbjct: 13  IAYVETDPSGRYGRFREVLGKGAMKTVYKAFDQVLGMEVAWNQVKLNEVFRSPEPLQRLY 72

Query: 65  SEVHLLKNLNHDSIIRFHASWIDTHRRTFNFITEMFTSGSLREYRQKYRNVDIGAIKNWA 124
           SEVHLLKNLNH+SIIR+  SWID +RRTFNFITE+FTSG+LREYR+KY+ VDI AIK+WA
Sbjct: 73  SEVHLLKNLNHESIIRYCTSWIDVNRRTFNFITELFTSGTLREYRRKYQKVDIRAIKSWA 132

Query: 125 RQILRGLVYLHEHDPPVIHRDIKCDNIFINGHLGQVKIGDLGLAAILHDSQHAHSVIGTP 184
           RQIL GL YLH HDPPVIHRD+KCDNIF+NGHLGQVKIGDLGLAAIL  SQ+AHSVIGTP
Sbjct: 133 RQILNGLAYLHGHDPPVIHRDLKCDNIFVNGHLGQVKIGDLGLAAILRGSQNAHSVIGTP 192

Query: 185 EFMAPELYDEEYNELVDVYSFGMCLIEMLTLEYPYSECSNPAQIYKKVTSGKLPNAFYKI 244
           EFMAPELY+E+YNELVD+YSFGMC++EMLT EYPYSEC+NPAQIYKKVTSGKLP++F+ I
Sbjct: 193 EFMAPELYEEDYNELVDIYSFGMCVLEMLTGEYPYSECTNPAQIYKKVTSGKLPDSFHLI 252

Query: 245 KGLEAQKFVRKCLENVSKRLPARELLLDPFLASND-----------------NASDNEEL 304
           +  EAQ+FV KCLE VS+RLPA+ELL DPFLA+ D                 N + N  +
Sbjct: 253 QHTEAQRFVGKCLETVSRRLPAKELLADPFLAATDERDLAPLFRLPQQLAIQNLAANGTV 312

Query: 305 CLSPTPKKSVARRTDMAISGSMNPKDNDKVFLKVQIKVKNGKAKNVYFAFDTINDTAIDV 364
                      R TDM+I+G MN +D+  +FL+VQI   +G  +N+ F F+ ++DT ++V
Sbjct: 313 VEHLPSTTDPTRTTDMSITGKMNSEDH-TIFLQVQILDGDGHMRNIQFPFNILSDTPLEV 372

Query: 365 ATEMVKELEISDWDPLEIAVMIKKEISTLIPDWEEWNIPKNHHQDSFNYDVGNDDDDDDD 424
           A EMVKELEI+DWDPLEIA MI+ EIS L+P+W   +    H  +SF    G++DD+D+ 
Sbjct: 373 ALEMVKELEITDWDPLEIAAMIENEISLLVPNWRANDSSIRH--ESF----GHEDDEDNG 432

Query: 425 DENNVTPHPFYSCSSHASSSNSLQGFYSLYDNPHHFGGRNGTSTATEWFQEDLLIDEDAS 484
           D    T        S ASSS         +D+P      N  S+       D++ D D  
Sbjct: 433 DTEGRT-----RLFSSASSS---------HDSPVAVRENNDDSS------NDVIPDMDDG 487

Query: 485 SSCSQNSFNYSDVSFDSNNEDD 490
           +  S    N S   +    +DD
Sbjct: 493 NRSSNRLLNSSTYHYSPAIDDD 487

BLAST of Lcy04g008290 vs. TAIR 10
Match: AT3G51630.2 (with no lysine (K) kinase 5 )

HSP 1 Score: 544.7 bits (1402), Expect = 9.5e-155
Identity = 297/502 (59.16%), Postives = 360/502 (71.71%), Query Frame = 0

Query: 5   IGYAETDPSGRYGRFREILGKGAMKTVYKAFDEVLGMEVAWNQVQLKDVFHSPEDLQRLY 64
           I Y ETDPSGRYGRFRE+LGKGAMKTVYKAFD+VLGMEVAWNQV+L +VF SPE LQRLY
Sbjct: 13  IAYVETDPSGRYGRFREVLGKGAMKTVYKAFDQVLGMEVAWNQVKLNEVFRSPEPLQRLY 72

Query: 65  SEVHLLKNLNHDSIIRFHASWIDTHRRTFNFITEMFTSGSLREYRQKYRNVDIGAIKNWA 124
           SEVHLLKNLNH+SIIR+  SWID +RRTFNFITE+FTSG+LREYR+KY+ VDI AIK+WA
Sbjct: 73  SEVHLLKNLNHESIIRYCTSWIDVNRRTFNFITELFTSGTLREYRRKYQKVDIRAIKSWA 132

Query: 125 RQILRGLVYLHEHDPPVIHRDIKCDNIFINGHLGQVKIGDLGLAAILHDSQHAHSVIGTP 184
           RQIL GL YLH HDPPVIHRD+KCDNIF+NGHLGQVKIGDLGLAAIL  SQ+AHSVIGTP
Sbjct: 133 RQILNGLAYLHGHDPPVIHRDLKCDNIFVNGHLGQVKIGDLGLAAILRGSQNAHSVIGTP 192

Query: 185 EFMAPELYDEEYNELVDVYSFGMCLIEMLTLEYPYSECSNPAQIYKKVTSGKLPNAFYKI 244
           EFMAPELY+E+YNELVD+YSFGMC++EMLT EYPYSEC+NPAQIYKKVTSGKLP++F+ I
Sbjct: 193 EFMAPELYEEDYNELVDIYSFGMCVLEMLTGEYPYSECTNPAQIYKKVTSGKLPDSFHLI 252

Query: 245 KGLEAQKFVRKCLENVSKRLPARELLLDPFLASND-----------------NASDNEEL 304
           +  EAQ+FV KCLE VS+RLPA+ELL DPFLA+ D                 N + N  +
Sbjct: 253 QHTEAQRFVGKCLETVSRRLPAKELLADPFLAATDERDLAPLFRLPQQLAIQNLAANGTV 312

Query: 305 CLSPTPKKSVARRTDMAISGSMNPKDNDKVFLKVQIKVKNGKAKNVYFAFDTINDTAIDV 364
                      R TDM+I+G MN +D+  +FL+VQI   +G  +N+ F F+ ++DT ++V
Sbjct: 313 VEHLPSTTDPTRTTDMSITGKMNSEDH-TIFLQVQILDGDGHMRNIQFPFNILSDTPLEV 372

Query: 365 ATEMVKELEISDWDPLEIAVMIKKEISTLIPDWEEWNIPKNHHQDSFNYDVGNDDDDDDD 424
           A EMVKELEI+DWDPLEIA MI+ EIS L+P+W   +    H  +SF    G++DD+D+ 
Sbjct: 373 ALEMVKELEITDWDPLEIAAMIENEISLLVPNWRANDSSIRH--ESF----GHEDDEDNG 432

Query: 425 DENNVTPHPFYSCSSHASSSNSLQGFYSLYDNPHHFGGRNGTSTATEWFQEDLLIDEDAS 484
           D    T        S ASSS         +D+P      N  S+       D++ D D  
Sbjct: 433 DTEGRT-----RLFSSASSS---------HDSPVAVRENNDDSS------NDVIPDMDDG 487

Query: 485 SSCSQNSFNYSDVSFDSNNEDD 490
           +  S    N S   +    +DD
Sbjct: 493 NRSSNRLLNSSTYHYSPAIDDD 487

BLAST of Lcy04g008290 vs. TAIR 10
Match: AT3G48260.1 (with no lysine (K) kinase 3 )

HSP 1 Score: 431.8 bits (1109), Expect = 8.9e-121
Identity = 217/382 (56.81%), Postives = 282/382 (73.82%), Query Frame = 0

Query: 7   YAETDPSGRYGRFREILGKGAMKTVYKAFDEVLGMEVAWNQVQLKDVFHSPEDLQRLYSE 66
           + E DP+GRYGR++E+LGKGA K VY+AFD++ G+EVAWNQV+L D F S EDL RLYSE
Sbjct: 12  FVEIDPTGRYGRYKEVLGKGAFKEVYRAFDQLEGIEVAWNQVKLDDKFCSSEDLDRLYSE 71

Query: 67  VHLLKNLNHDSIIRFHASWIDTHRRTFNFITEMFTSGSLREYRQKYRNVDIGAIKNWARQ 126
           VHLLK L H SII+F+ SWID    T N ITE+FTSG+LR+YR+K++ VD+ A+K W+RQ
Sbjct: 72  VHLLKTLKHKSIIKFYTSWIDHQHMTINLITEVFTSGNLRQYRKKHKCVDLRALKKWSRQ 131

Query: 127 ILRGLVYLHEHDPPVIHRDIKCDNIFINGHLGQVKIGDLGLAAILHDSQHAHSVIGTPEF 186
           IL GLVYLH HDPPVIHRD+KCDNIFING+ G+VKIGDLGLAAILH ++ AHSVIGTPEF
Sbjct: 132 ILEGLVYLHSHDPPVIHRDLKCDNIFINGNQGEVKIGDLGLAAILHRARSAHSVIGTPEF 191

Query: 187 MAPELYDEEYNELVDVYSFGMCLIEMLTLEYPYSECSNPAQIYKKVTSGKLPNAFYKIKG 246
           MAPELY+E+YN LVD+Y+FGMCL+E++T EYPYSEC+N AQIY+KVTSG  P A   +  
Sbjct: 192 MAPELYEEDYNVLVDIYAFGMCLLELVTFEYPYSECTNAAQIYRKVTSGIKPAALLNVTD 251

Query: 247 LEAQKFVRKCLENVSKRLPARELLLDPFL----ASNDNASDNEELCLSP---TPKKSVAR 306
            + + F+ KC+  VS+RL A+ELL DPFL     + +N S ++E   +      K S + 
Sbjct: 252 PQVRAFIEKCIAKVSQRLSAKELLDDPFLKCYKENTENVSSHKENGYNGNGIVDKLSDSE 311

Query: 307 RTDMAISGSMNPKDNDKVFLKVQIKVKNGKAKNVYFAFDTINDTAIDVATEMVKELEIS- 366
              + + G    KD + +FLK++I    G+ +N++F F+   DT+  VA EMV+EL+++ 
Sbjct: 312 VGLLTVEGQR--KDLNTIFLKLRITDSKGQIRNIHFPFNIETDTSFSVAIEMVEELDLTD 371

Query: 367 DWDPLEIAVMIKKEISTLIPDW 381
           D D   IA MI  EI + IPDW
Sbjct: 372 DQDISTIAKMIDTEIHSHIPDW 391

BLAST of Lcy04g008290 vs. TAIR 10
Match: AT3G22420.1 (with no lysine (K) kinase 2 )

HSP 1 Score: 431.0 bits (1107), Expect = 1.5e-120
Identity = 226/480 (47.08%), Postives = 310/480 (64.58%), Query Frame = 0

Query: 7   YAETDPSGRYGRFREILGKGAMKTVYKAFDEVLGMEVAWNQVQLKDVFHSPEDLQRLYSE 66
           + E DPSGRYGR+ EILGKGA KTVY+AFDE  G+EVAWNQV+L++   +PE+L++ + E
Sbjct: 14  FVEIDPSGRYGRYDEILGKGASKTVYRAFDEYEGIEVAWNQVKLRNFTRNPEELEKFFRE 73

Query: 67  VHLLKNLNHDSIIRFHASWIDTHRRTFNFITEMFTSGSLREYRQKYRNVDIGAIKNWARQ 126
           +HLLK LNH +I++F+ SW+DT+  + NF+TE+FTSG+LR+YR ++R V+I A+K W +Q
Sbjct: 74  IHLLKTLNHQNIMKFYTSWVDTNNLSINFVTELFTSGTLRQYRLRHRRVNIRAVKQWCKQ 133

Query: 127 ILRGLVYLHEHDPPVIHRDIKCDNIFINGHLGQVKIGDLGLAAILHDSQHAHSVIGTPEF 186
           IL+GL+YLH   PP+IHRD+KCDNIFING+ G+VKIGDLGLAAIL  S HA   +GTPEF
Sbjct: 134 ILKGLLYLHSRSPPIIHRDLKCDNIFINGNQGEVKIGDLGLAAILRKS-HAVRCVGTPEF 193

Query: 187 MAPELYDEEYNELVDVYSFGMCLIEMLTLEYPYSECSNPAQIYKKVTSGKLPNAFYKIKG 246
           MAPE+YDEEYNELVDVY+FGMC++EM+T +YPYSEC++PAQIYKKVTSGK P AFY +K 
Sbjct: 194 MAPEVYDEEYNELVDVYAFGMCVLEMVTFDYPYSECTHPAQIYKKVTSGKKPEAFYLVKD 253

Query: 247 LEAQKFVRKCLENVSKRLPARELLLDPFLASND--------------------------- 306
            E ++FV KCL NV+ RL A ELL DPFL  ++                           
Sbjct: 254 PEVREFVEKCLANVTCRLTALELLQDPFLQDDNMDGFVMRPIDYYNGYDETGVFLRHPLI 313

Query: 307 ------NASDNEELCLSPTPKKSVARRTDMAISGSMNPKDNDKVFLKVQIKVKNGKAKNV 366
                 +  ++ ++C             D++I G  N   +D +FL+++I    G+ +N+
Sbjct: 314 DDPLYHDQFESSQICEIDLFANDDEDHVDISIKGKRN--GDDGIFLRLRISDAEGRIRNI 373

Query: 367 YFAFDTINDTAIDVATEMVKELEISDWDPLEIAVMIKKEISTLIPDWEEWNIPKNHHQDS 426
           YF F+T  DTA  VA EMV EL+I++ D  +IA MI  EI+ L+PDW      KN  + S
Sbjct: 374 YFPFETAIDTAWSVAVEMVSELDITNQDVAKIAEMIDAEIAALVPDW------KNDTESS 433

Query: 427 FNYDVGNDDDDDDDDENNVTPHPFYSCSSHASSSNSLQGFYSLYDNPHHFGGRNGTSTAT 454
            N          ++++NN T      C+S+     ++      + N H F      S ++
Sbjct: 434 QNV---------NNNKNNNTAGFCGECASNGYIQETVSSGEKSHHNHHEFDSSEDKSCSS 475